CELLULAR RESPIRATION ACTIVITY

Myisha V. Garcia Grade 11 - Pasteur

Activity 1:
1. What is Cellular respiration?
a. Metabolic reactions in the cell that are designed to convert nutrients from food into
Adenosine Triphosphate (ATP)
b. —Cellular respiration involves 3 different cycles:
i. —Glycolysis
ii. —Citric Acid cycle or “Kreb’s” cycle
iii. Oxidative phosphorylation
2. Differentiate aerobic from anaerobic cellular respiration.
a. Aerobic Cellular Respiration
i. Cellular respiration that proceeds in the presence of oxygen
ii. Both glycolysis and Kreb's cycle takes place in this type of respiration.
iii. It provides more energy i.e. 38 ATP molecules
iv. The end products are CO2 and H2O
b. Anaerobic Cellular Respiration
i. Cellular respiration that proceeds in the absence of oxygen
ii. Only glycolysis occurs in this type of respiration.
iii. It provides less energy i.e. 2 ATP molecules—
iv. The end products are either ethyl alcohol or lactic acid and CO2
3. What are the major stages of Aerobic Cellular respiration? Give a brief
description of how the process works.
a. Glycolysis
i. Phosphorylation and splitting of glucose
b. Pyruvate Oxidation (Link Reaction)
i. Decarboxylation and dehydrogenation of pyruvate
c. Citric Acid Cycle (Kreb's Cycle)
i. Cyclical pathway with enzyme - controlled reactions
d. Oxidative Phosphorylation
i. Production of ATP through oxidation of hydrogen atoms

Activity 2:
Activity 2.1
Summarize the process of Glycolysis by completing the table below.

REACTANTS PRODUCTS ATP ATP YIELD NET ATP

REQUIRED YIELD

Glucose 2 2 ATP 4 ATP 2 ATP

2 ATP pyruvate
GLYCOLYSIS 2 NAD+ 4 ATP
4 ADP 2 NADH
 2 Pi 2 H+
2 ADP

Activity 2.2
Summarize the process of Pyruvate Oxidation by completing the table below.

REACTANTS PRODUCTS ATP ATP YIELD NET ATP

REQUIRED YIELD

PYRUVATE 2 2 CO2 None None None

Pyruvate 2 NADH
OXIDATION
2 NAD+ 2 Acetyl-
2 CoA CoA
2 H+

Activity 2.3
Summarize the process of Citric Acid Cycle by completing the table below.

REACTANTS PRODUCTS ATP ATP NET ATP

REQUIRED YIELD YIELD

CITRIC 2 Acetyl-CoA 2 CoA None 2 ATP 2 ATP

2 4 CO2
ACID
Oxaloacetate 2
CYCLE 6 NAD+ Oxaloacetate
2 ADP 6 NADH
2 Pi 6 H+
2 FAD 2 FADH2
2 ATP

Activity 2.4
Summarize the process of Oxidative Phosphorylation  by completing the table below.

REACTANTS PRODUCTS ATP ATP NET ATP

REQUIRED YIELD YIELD

OXIDA 6 NADH 8 NAD+ None 32 32 ATP

(Krebs) 4 FAD+ ATP
TIVE
2 NADH 24 H+
PHOSP (pyruvate 32 ATP
HORYL oxidation) 6 H2O
2 FADH2
ATION
(Krebs)
2 FADH2
(from 2
cytosolic
NADH)
 32 ADP
32 Pi
6 O2
12 H+

Activity 2.5
Summarize the overall process of Aerobic Cellular Respiration by completing this table.

GLYCOLYSIS PYRUVATE CITRIC ACID OXIDATIVE

OXIDATION CYCLE PHOSPHORYLATI
ON

LOCATION Cytoplasm Mitochondrial Mitochondrial Mitochondrial

matrix matrix and inner matrix and
membrane intermembrane
space

REACTANTS Glucose 2 Pyruvate 2 Acetyl-CoA 6 NADH (Krebs)

2 ATP 2 NAD+ 2 Oxaloacetate 2 NADH
2 NAD+ 2 CoA 6 NAD+ (pyruvate
4 ADP 2 ADP oxidation)
2 Pi 2 Pi 2 FADH2 (Krebs)
2 FAD 2 FADH2 (from 2
cytosolic NADH)
32 ADP
32 Pi
6 O2
12 H+

PRODUCTS 2 pyruvate 2 CO2 2 CoA 8 NAD+

4 ATP 2 NADH 4 CO2 4 FAD+
2 NADH 2 Acetyl-CoA 2 Oxaloacetate 24 H+
2 H+ 2 H+ 6 NADH 32 ATP
2 ADP 6 H+ 6 H2O
2 FADH2
2 ATP

ATP 2 ATP None None None

REQUIRED

ATP YIELD 4 ATP None 2 ATP 32 ATP

NET ATP 2 ATP None 2 ATP 32 ATP

YIELD