Sifting and Winnowing the Evidence in Veterinary Parasitology

Joint Meeting With The 64th American Association of Veterinary Parasitologists Annual Meeting
& The 63rd Annual Livestock Insect Workers Conference

27th Conference of the World Association for

the Advancement of Veterinary Parasitology
July 7 - 11, 2019 | MADISON, WI, USA

Efficacy variation of Mentha piperita essential oil on Haemonchus contortus isolates with
differing benzimidazole resistance backgrounds
2 2 1
Fernando Raul Correia de Vasconcelos ; Janaelia Ferreira Vasconcelos ; Jomar Patrício Monteiro ; Luiz da Silva Vieira ; 1,2
1,2
Marcel Teixeira Embrapa Caprinos e Ovinos / Brazil 1; Master's Program in Biotechnology / University Center UNINTA / Brazil 2
marcel.teixeira.vet@gmail.com / jomarmont@gmail.com

Introduction the maximum concentration tested (1.0 mg/mL). The EC50

was calculated by probit analysis (table 1).
The spread of anthelmintic resistance made treatment
options against gastrointestinal parasites increasingly scarce
so the search for natural compounds as antiparasitic Table 1 - EC50 and confidence limits of EHT (probit analysis).
candidates is now an important field. However, because
EC50 thiabendazole EC50 essential oil
concentrations of active compounds may vary according to Isolate (µg/mL) (µg/mL)
their origin and post-harvest processing, there are many
obstacles for the establishment of standards hindering Resistant 2.87 (1.47 - 43.75 (38.28 -
reliable results. To date it is not known if a given compound 20.25) 50.12)
may vary on efficacy between parasite populations.
Non 0.067 (0.040 - 6934.26
Discussion
resistant 0.106) (4645.15 -
Objective 11040.79)
Our results suggest that the components in Mentha piperita
essential oil probably have a different mode of action in
To evaluate the efficacy of Mentha piperita essential oil gastrointestinal nematodes as the resistance background to
against eggs of two Haemonchus contortus isolates with benzimidazole was not associated with EHT results for the
and without history of resistance to benzimidazoles. studied isolates.

Conclusion
Methods
We concluded that is important to test natural compounds
Haemonchus as antiparasitic candidates on more than one nematode
contortus isolate Eggs Hatch Test (EHT) population in order to provide a better overview of the
with history of ~150 eggs/well
benzimidazole actual efficacy.
resistance

Haemonchus Acknowledgements
contortus isolate
without history of ~150 eggs/well
benzimidazole
resistance
Mentha piperita essential oil
(1.0 - 0.004 mg/mL)

+
Polysorbate 80 References
(2%)
COLES, G.C., et al., 1992. World Association for the Advancement of Veterinary
Results Parasitology (WAAVP) methods for the detection of anthelmintic resistance in
nematodes of veterinary importance. Veterinary Parasitology. 44, 35–44.
COLES G.C., et al., 2006. The detection of anthelmintic resistance in nematodes of
EHT results for the resistant isolate showed 99.5% efficacy veterinary importance. Veterinary Parasitology. 136, 167-185.
at concentrations above 0.5 mg/mL. However, the EHT KATIKI, L. M. et al. 2011. Anthelmintic activity of Cymbopogon martinii,
results for the sensitive isolate were much lower at 32.3% Cymbopogon schoenanthus and Mentha piperita essential oils evaluated in four
different in vitro tests. Veterinary Parasitology. 183, 103-108.
at 0.5 mg/mL and 41.8% efficacy at