LBYBION

2022–2023 T2
EXERCISE 2: THE COMPOUND MICROSCOPE
SECTION/GROUP: A52/Group 1 SCORE:___/60

Section A. Familiarization with Parts of the Microscope

A.1 Label the parts of the microscope in the image below: (12 points)

EYE PIECE

OBJECTIVES

STAGE

CONDENSER
COARSE FOCUS

FINE FOCUS
LIGHT
SOURCE

Section B. Familiarization on How to Use the Microscope

B.1 Not included in the parts of the microscope above is the field diaphragm. What is its
function and how is it used? (2 pts)
B.2 What is the use of the oil for the oil immersion objective? (2 pts)

B.3 In storing your microscope, what objective should be in place? Why should this be
done? (4 pts)

B.4 Letter e under the microscope:

1. Draw the letter e under the scanner (4x). (2 pts)

2. Draw the letter e under the low power objective (10x). (2 pts)

3. Draw the letter e under the high power objective (40x). (2 pts)

B.5 Answer the following questions:

1. Is the position of the letter e the same as when viewed with the naked eye or is it
inverted? (2 pts)

2. When the slide is moved to the left, in which direction does the letter image
appear to move? (2 pts)

3. When the slide is moved towards you, in which direction does the letter image
appear to move? (2 pts)

4. When the objective is shifted to HPO, what happens to the letter image and field
of view? (2 pts)

5. Explain your observations. (2 pts)

Section C: Measuring Objects under the Microscope with and without using a
Micrometer

C.3 Given these images focused under different objectives, calculate the size of an
onion cell and a Bangka-bangkaan cell. Show your calculations. (8 pts)

(HPO) (LPO)

Fig. A. Onion (Allium sp.)

Size: _________mm
Size: _________um
Calculation:

Fig. B. Bangka-bangkaan (Tradescantia sp.)

Size: _________mm
Size: _________um
Calculation:
Section D: Measurement of Magnification of Images

D.1 What is the total magnification using the following objectives using your
microscope?
Show your calculations: (8 pts)

Magnification. Total Magnification:

Scanner __________ ___________
Low power objective ___________ ___________
High power objective ___________ ___________
Oil immersion objective ___________ ___________

D.2 Magnification of a drawing

a. Compute the magnification of the two images of the Onion and Bangka-bangkaan
cells above (Refer to Section C.3).

b. Get a grain of rice and a foot ruler. Measure their actual lengths in mm.

c. Compute the magnification of a 5 cm drawing of rice grain and foot ruler.

Specimen Actual size Magnification

(mm) (Show computation)

Onion cell (length) (Refer to Section C.3 Fig. Magnification as shown in the
A) (2pts) image above Section C.3 Fig.
A
(2pts)

Bangka-bangkaan cell (Refer to Section C.3 Fig. Magnification as shown in the

(length) B) image above Section C.3 Fig.
(2 pts) B
(2pts)
 Foot ruler (length) (Actual measurement) Magnification of a 5-cm
(2 pts drawing (2pts)

Rice grain (length) (Actual measurement) Magnification of a 5-cm

(2pts) drawing (2pts)

Table of Contributions: Indicate the work contribution of the group members below.
Member with no participation will be graded zero (0).

Name Contribution Remarks

1.

2.

3.

4.

5.