Cryosectioning

A 101 How To?

What is Cryosectioning? And Why?
- Slicing Organic Materials into very thin
slices to study

- Requires precise hand movements to

preserve details of organic slices

- Specifically, for myself, Jacque, and Jona,

we were slicing heart samples in order to
study the tissue on a microscopic level

- Cryosectioning allows us to view the

cardiomyocytes frozen in time and see the
detailed cellular changes in the hearts
over time.
Basic Steps
1) Retrieve sample

2) Mount to sample in OCT onto chuck

and slice for about 5 μm

3) Once sliced, using a polarized

microscopic plate, mount the slice.
There should be multiple slices on
one plate to be as efficient as
possible

4) Once mounted, fix the slides with

PBST
Basic Steps cont.
5) Once fixed, we used Troponin as the
primary antibody to stain the
cardiomyocytes via Immunostaining.

6) After immunostaining, we can start

looking into microscopy and data
analysis of the cardiomyocytes
Results & Next Steps
Unfortunately, I was not able to get to
fully immunostaining and analyzing the
cardiomyocytes under the microscope.

Will be working on recollecting and

creating a more concrete procedure to
head into the next school year and
working on how to analyze the
cardiomyocytes
THANK YOU