Professional Documents
Culture Documents
Research I
Quarter 4 – Module 1:
Research Proposal
(Methodology and Bibliography)
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Research I
Quarter 4 – Module 1:
Research Proposal
(Methodology and Bibliography)
Introductory Message
This Self-Learning Module (SLM) is prepared so that you, our dear learners,
can continue your studies and learn while at home. Activities, questions, directions,
exercises, and discussions are carefully stated for you to understand each lesson.
Each SLM is composed of different parts. Each part shall guide you step-by-
step as you discover and understand the lesson prepared for you.
Pre-tests are provided to measure your prior knowledge of lessons in each
SLM. This will tell you if you need to proceed with completing this module or if you
need to ask your facilitator or your teacher’s assistance for a better understanding
of the lesson. At the end of each module, you need to answer the post-test to self-
check what you are learning. Answer keys are provided for each activity and test. We
trust that you will be honest in using these.
In addition to the material in the main text, notes to the Teacher are also
provided to our facilitators and parents for strategies and reminders on how they
can best help you with your home-based learning.
Please use this module with care. Do not put unnecessary marks on any part
of this SLM. Use a separate sheet of paper in answering the exercises and tests. And
read the instructions carefully before performing each task.
If you have any questions in using this SLM or any difficulty in answering the
tasks in this module, do not hesitate to consult your teacher or facilitator.
Thank you.
What I Need to Know
This module was designed and written with you in mind to help you master
the nature of the research proposal. In the previous module, the introduction,
formulation of a research question, and hypothesis have been introduced. Now the
students are ready to write a detailed description of the procedures to be
undertaken, and list all sources cited in their study. The scope for this module
permits it to be used in many different learning situations. The language used
recognizes the diverse vocabulary levels of students. The activities are arranged to
follow the standard sequence of the course. However, the order in which you read
them can be changed to correspond with the textbook you are now using.
What I Know
Directions: Read each question carefully. Choose the letter of the correct answer.
1. Which of the following best describes the methodology in a proposal?
a. a diagram of how the project will be done
b. a statement of the dos and don’ts of the experiment
c. a written structure of the data gathered in the experiment
d. a written plan on how the experimentation will be conducted
2. Which of the following is NOT a part of the methodology?
a. sampling technique
b. analyses of the samples
c. the result from the data collected
d. all materials and amount to be used
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3. Which is the correct way to write if the sentence starts with a number?
a. 10 grams of ipil leaves c. Ten (10) grams of ipil leaves
b. Ten grams of ipil leaves d. both a and b
4. Which statement is written correctly (pronounce use) in methodology?
a. I will analyze the samples for the antibacterial property of lemongrass.
b. He/she will analyze the samples for the antibacterial property of
lemongrass.
c. The researcher will analyze the samples for the antibacterial property
of lemongrass.
d. The researcher analyzed the samples for the antibacterial property of
lemongrass.
5. Which of the following is NOT a safety practice in experimenting?
a. wearing gloves, goggles, and a lab coat
b. immediate decontamination after spills
c. slight inhalation of solvents and chemicals not labeled
d. disposal of chemicals and sharp tips in proper waste containers
6. Which is the best way to present the procedures in the methodology?
I. Procedures are enumerated or listed as in the laboratory manual.
II. Materials to be used are mentioned as the details of the procedure are given.
III. Headings and subheadings help logically direct the researcher to the steps
to follow.
IV. Procedures are given in the order that they should be done in the actual
conduct of the experiment.
a. I and II c. I, II, and III
b. III and IV d. II, III, and IV
7. Which of the following are excellent sources for the methodology?
a. laboratory manuals c. personal experiences
b. published sources d. replication of prior researches
8. Which of the following is TRUE about bibliography?
a. reading materials not cited in the research
b. compilation of related studies for the last 10 years
c. documents the written sources of information used in the study
d. list of all books, papers, journal articles, and communication about the
research
9. Which of the following should NOT be in the bibliographic entry?
a. blogs c. journals
b. books d. conference papers
10. Which of the following statements is written correctly (verb tense) in the
methodology section of the research proposal?
a. The coir fibers were obtained from the shells of randomly selected
mature coconuts.
b. The coir fibers will be obtained from the shells of randomly selected
mature coconuts.
c. The coir fibers had been obtained from the shells of randomly selected
mature coconuts.
d. The coir fibers are being obtained from the shells of randomly selected
mature coconuts.
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Lesson Research Proposal
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The presentation of the Research Proposal in this module is focused on these
elements:
I. Methodology, a detailed description of the following:
1. Procedures
a. Materials
b. Methods
2. Risk and Safety Considerations
3. Data Analysis
II. Bibliography
Thus, specific discussions are labeled in a pre-laboratory perspective, a guide
before conducting the research. Generally, the discussions are written in the future
tense of the verb.
More so, the methods in the procedure part are divided into several sections:
collection and preparation to be done, sampling procedure, and the analyses to be
performed with the samples. Likewise, the bibliographic entries should be written in
the required American Psychological Association (APA) format.
What’s In
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Activity I: My Proposal
Directions: Read and answer the following guide questions related to the proposed
study which was approved by your teacher.
Guide questions:
1. What is the working title?
5. Write one related study (follow the APA Reference Format 7th ed. below) and
point out clearly how the cited study is related to your problem.
APA Book Format: Author, A., & Author, B. (Copyright Year). Title of the book
(7th ed.). Publisher. DOI or URL
APA Journal Article: Author, A., & Author, B. (Year). Title of the article.
Name of the Periodical, volume(issue), #-#, https://doi.org./xxxx
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What’s New
Before conducting experimental research, a work plan plays a very vital role.
The methodology in the research proposal aims to inform the readers of the steps to
be undertaken by the researcher: the proposed procedures and materials, the
experimental design, collection and analysis of data, and the risks and safety to be
considered in the conduct of the experimentation.
Directions: Read the excerpt methodology carefully and answer the guide questions
that follow.
Methodology
Paragraph 1:
Peeling of saba and corn are to be collected, sundried, and cut. The used bond
paper will also be gathered. The used paper will be pulped in sodium hydroxide.
Saba and corn peeling pulp will be bleached.
Paragraph 2:
Five treatments will be prepared using the bleached saba, corn, and paper
pulps: Treatment A (100% recycled paper); Treatment B (50% corn + 50% recycled
paper); Treatment C (50% saba +50% recycled paper; and Treatment D (25%
corn
+ 25% saba + 50% recycled paper).
Paragraph 3:
Binding solutions will be added to the treatments, after which each of the
treatments will be allowed to dry. Five replicates of each setup will be prepared,
making a total of 20 hand sheets. The resulting hand sheets will be compared in
terms of folding endurance, tearing strength, and permeability or the extent that
an ink mark can penetrate a sample sheet.
Paragraph 4:
The use of certain chemicals containing strong acid and bases may pose threat;
therefore, proper handling of the set-up and use of personal protective equipment
will be always observed. Proper disposal of the expected wastes and discard of the
excess chemicals should also be planned well in the conduct of the experiment.
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Guide Questions:
What is It
I. Methodology
1.1 Content
The methodology section of the research proposal informs the reader of the
steps to be undertaken and how they will be done. It includes the following:
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1.2 Organization, Style, and Format
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Example:
General Procedure
1. Formulation of Test Pieces
Three formulations will be prepared as shown in Table 1.
2. Forming of Test Pieces
The mixture (20 g) will then…
3. Air Drying
The pressed discs will be…
e. Describe how the data will be analyzed. Indicate what types of data
analyses to be employed to answer each of the questions or hypotheses
to be tested. The information should include statistical tests to be used
about the questions to be addressed.
Example:
"A Paired t-test will be used to compare mean flight duration
before and after applying stabilizers to the glider's wings."
"One way ANOVA will be used to determine whether both the
treatment and the extracts showed a significant difference from each
other.”
“T-test is to be used to separate a set of significant difference means
into subsets of the homologous means to further recognize which are
different.”
f. Abbreviations are never used on the first citations. The same rule
applies to scientific names. They are spelled out completely on the first
citation; however, abbreviations may be used in the later citation.
Example:
Escherichia coli is spelled out in the first citation while the
abbreviated E. coli is written in the succeeding citations.
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h. If the sentence starts with a number, the number is to be spelled out.
Example:
Ten grams of malunggay leaves will be mixed with 50 ml of tap
water to prepare an aqueous solution extract of the sample.
II. Bibliography
This section documents the written sources of information used in the study.
It includes pertinent resource materials in the research proposal. It lists all books,
papers, journal articles, and communication cited in the paper. A common oversight
error among many research papers is the non-inclusion of cited works in the
bibliography. Sometimes, cited materials included in the Review of Related
Literature are not listed. (Rabago, Joaquin, and Cruz 2001, 43)
The most practical and useful way to arrange the bibliographic entries is in
alphabetical order, listed by authors. For a more convenient listing, use the
following categories:
I. Books
II. Journals
III. Periodicals
IV. Conference Papers
V. Websites
I. Books
Cottrell, S. (2013). The study skills handbook (5th ed.). Basingstoke: Palgrave
Macmillan.
Franks, A. (2005). Margaret Sanger's eugenic legacy: The control of female fertility.
McFarland & Company.
II. Journals
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For further clarification on how to write a bibliography of the research paper,
consult the Publication Manual of the American Psychological Association (7th ed.).
This is the prescribed bibliographic format being followed in the International
Science and Engineering Fair (ISEF).
Methodology
The materials which will be needed for conducting the experiment are
triclosan E. coli (Strain k-12), tryptic soy agar plates, tryptic soy broth,
ethanol, distilled water, cotton swabs, filter paper, forceps, incubator, paper
disks, and 6 locally available antibiotics.
Streptomycin
Sulfa m e t
hoxa z ol e
Triclo-
water
san
E. coli
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Procedures and Data Analysis
1. Prepare Plates of Bacteria
Transfer E. coli (Strain k-12) from stock cultures to tryptic soy
agar plates. Spread the bacteria uniformly on the plate to make a
bacterial lawn (Welden and Hossler, 2003).
2. Prepare Triclosan Solution
Dissolve Triclosan powder in a solution of 17.5% ethanol and 82.5%
distilled water (triclosan concentration of 500 µg/ml.) (Do different
dilutions of disinfectants affect the development of bacterial
resistance?, 2007).
3. Make Bacteria Resistant to Triclosan
Transfer bacteria from a liquid culture into an agar plate. Apply a
paper disk soaked in triclosan solution to the tryptic soy agar plate with
E. coli. Incubate 24 hours at 37°C. Measure the width of the zone of
inhibition. The zone of inhibition is the diameter from the one side of
the inner margin of the bacterial lawn to the other side of the inner
margin of the bacterial lawn. Isolate the bacteria most resistant to
triclosan from each plate by rubbing a sterile cotton swab across the
inner margin of the zone of inhibition. Transfer the bacteria to liquid
culture. Incubate for 24 hours at 37°C. Repeat between 6-10 times
(Welden and Hossler, 2003).
4. Test Bacterial Resistance to Antibiotics.
Dip a sterile cotton swab into the liquid culture of the triclosan
resistant bacteria. Spread the bacteria uniformly on the plate to make
a bacterial lawn. Place a magazine disk of antibiotics into the center of
the plate. Incubate for 24 hours at 37°C.
5. Measure Zone of Inhibition
The zone of inhibition around each disk will be measured. If the
zone of inhibition is 10 mm or less the bacteria is resistant to the
antibiotic, if the zone of inhibition is 11-l mm the bacteria have
intermediate resistance to the antibiotic, and if the zone of inhibition is
16mm or more, the bacteria is susceptible to the antibiotic (Do different
dilutions of disinfectants affect the development of bacterial
resistance?, 2007).
6. Data Analysis
The effect of cell concentration on antibiotic activity will be
analyzed with the t-test. The data which will be collected independently
from the samples will compare their measurements using analysis of
variance (ANOVA).
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Risk and Safety
Senese, F. (1999, June 27). What are triclocarban and triclosan (ingredients
in some antiseptic soaps)? In Chemistry of everyday life. Retrieved
August 5, 2008, from General chemistry online Web site:
http://antione.frostburg.edu/chem/senese/101/consumer/faq/print-
triclosan.shtml.
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Welden, C. W., & Hossler, R. A. (2003, January). Evolution in the lab: Biocide
resistance in E. coli. The American Biology Teacher, 65(1),56-61.
Source: Adapted from the Research Plan, “The Effects of Triclosan Resistant E.
coli Bacteria when Exposed to Common Antibiotics.” (“Info for All,” n.d.)
What’s More
Directions: Read the excerpt methodology and bibliography sections from a
research plan. Analyze whether the content and format are followed correctly.
Methodology
Two bags of empty and discarded mango pods will be collected from the
province and another two bags of sawdust will be obtained from lumberyard.
The mango pods will be shredded to the desired size and the following mango
pod (MP): sawdust (SD) mixtures (proportion determine by weight) will be
prepared: 3:1. 1:3, 1:1, 4:0, 0:4. Each setup will be packed in bags, each 310
g of substrate. Enough water will be added until moisture level of about 65%-
70% is reached. The bags of substrate will be given a week for the component
pods and sawdust mixtures and water to set. Five replicates of each setup will
be prepared, making a total of 25 bags.
The bags will be sterilized, allowed to cool, then inoculate aseptically
with 1 inch X 1 inch block of oyster mushroom spawns obtained from NIST.
The bags will be stored in a fruiting cabinet with temperature, light, and
moisture control (OYSTER MUSHROOM, 2021). Daily observations from
the mycelia growth will be done although the mushrooms are expected to
sprout after an incubation period of 30 days.
The observation and recording of data will focus on the following:
1. Substate where basidiocarps will first appear
2. Color and general appearance of basidiocarps
3. Sampling of mushrooms from each setup for cooking and tasting
4. Counting the number of sprouts from each setup and averaging the
replicates
5. Harvesting, weighing, and averaging the weights of the replicates
Bibliography
Alam, N., Amin, R., Khan, A., Ara, I., Shim, M. J., Lee, M. W., Lee, U. Y., &
Lee, T. S. (2009). Comparative Effects of Oyster Mushrooms on Lipid
Profile, Liver and Kidney Function in Hypercholesterolemic Rats.
Mycobiology, 37(1), 37–42.
https://doi.org/10.4489/MYCO.2009.37.1.037
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Cont. of Bibliography
Source: Adapted from the Research Plan, “Mongo Pods as Medium for Growing
Oyster Mushrooms (Pleurotus ostreatus).”
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Activity 2: What’s Wrong
Directions: For the items marked under NO answers in Activity 1, explain and
provide the appropriate content and format.
A. Methodology:
B. Bibliography:
Directions:
1. Use prior knowledge about the survey of literature and related studies.
2. Find at least three (3) references that will provide you with the
appropriate methodology for your research proposal.
3. Use science journal articles, books, published/conference papers, and
internet sites.
4. Follow specific content, format, and style in writing a methodology.
5. Be guided with the following specifications in finding the related
studies/literature for your methodology:
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What I Have Learned
Directions: Fill in the blank to complete the statements with the correct words/terms
provided in the box.
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What I Can Do
Congratulations on reaching this section- the detailed procedures on how
the experimentation will be performed and the cited works in the bibliography.
Directions: Follow through with the approved topic and introduction that you have
developed in the previous module, write the appropriate methodology for
the research proposal, and list all the cited sources in the bibliography
sections. Be guided using the rubric for the research plan. (Note:
Modified version of the research proposal template and rubric are
provided below).
Title/Topic
Hypothesis/Engineering Goals
Rationale
A brief synopsis of the background that supports your research problem and
explains why this research is important scientifically and, if applicable, explain your
research's societal impact.
Review Related Literature (IMRAD format includes this part in the introduction,
however, it is a good practice to have a file of the sources used in the study).
Provide an overview of sources you have explored while researching a particular topic
and demonstrate how the current study fits within a larger field of study.
Materials List
List of ALL items used in research. Ensure concentrations of all chemicals, source
and amount of all living organisms, and all equipment used.
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Procedures (Research Methods)
DETAIL description of method/procedures, risk and safety, and proper disposal (if
needed).
Bibliography
List at least five (5) major references (e.g., scientific journal articles, books,
internet sites) from the literature review. Please use a variety of sources; five
sources from the internet will NOT suffice.
Source: https://studyres.com/doc/6525986/rsef-research-plan-template
https://www.societyforscience.org/
Tentative/Working Title:
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Control & experimental group identified & designed
correctly (2)
Repeated Trials used (2)
If Engineering
Clear building plan (thought was given to materials) (4)
Method of testing (4)
D. Bibliography
Minimum of at least 5 science/engineering major
journal articles, must pertain to project topic in
proper APA format (10) (minus 1 point for each error
in references, max 5 points)
No spacing within citation, single space between citations 12
(2)
2-point bonus for 10 or more references (can only receive
once)
E. Format
FUTURE tense (2)
A, B, C, D Format (2) 10
Times New Roman/Size 12 font, Double Spaced (2)
Title – Bold-faced and centered (2)
Research Plan is written above title (2)
Total Points Earned 50
Source: commackschools.org
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Assessment
Additional Activities
Reflect on this:
Directions: Evaluate three (3) of the references listed in the bibliography following
the guide questions:
1. What background information among research studies is related to your current
study?
2. Are the recommendations stated in the related study relevant to your stated
problem?
3. What innovation is presented in your study as comparedto the related studies?