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  • Common HPLC Problems and Probable Causes

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    Ricardo Batista
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    This document lists common problems encountered in high performance liquid chromatography (HPLC) and their probable causes. It identifies symptoms such as high or low pressure, noisy or wandering baselines, no or bad peaks, issues with retention time, peak area, and accuracy. For each symptom, it provides the most likely causes, such as problems with the column, filter, pump, lamp, mobile phase, detector, injector, connections, autosampler, temperature changes, degassing, or sample preparation.

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    This document lists common problems encountered in high performance liquid chromatography (HPLC) and their probable causes. It identifies symptoms such as high or low pressure, noisy or wandering baselines, no or bad peaks, issues with retention time, peak area, and accuracy. For each symptom, it provides the most likely causes, such as problems with the column, filter, pump, lamp, mobile phase, detector, injector, connections, autosampler, temperature changes, degassing, or sample preparation.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    22 views2 pages

    Common HPLC Problems and Probable Causes

    Uploaded by

    Ricardo Batista
    This document lists common problems encountered in high performance liquid chromatography (HPLC) and their probable causes. It identifies symptoms such as high or low pressure, noisy or wandering baselines, no or bad peaks, issues with retention time, peak area, and accuracy. For each symptom, it provides the most likely causes, such as problems with the column, filter, pump, lamp, mobile phase, detector, injector, connections, autosampler, temperature changes, degassing, or sample preparation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    Common HPLC

    Problems and
    Probable Causes
    Symptom Most Probable Cause

    Pressure Too high Column, filter


    Too low Leaks from column, pump
    Baseline Noisy Old lamp, mobile phase
    Degassing, mobile phase,
    Wandering contaminated column
    Peak No peak Detector, injector
    Column, mobile phase,
    Bad peaks connections
    Data Retention Degassing, column, temperature
    performance time changes,
    insufficient equilibration time
    Autosampler, column, integration,
    Peak area lamp
    Accuracy Sample prep, autosampler

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