Anand Institute of Higher Technology

A
Department of Biotechnology

Monthly Test-I March 2023

Year/Sem: II/IV Course Title: Industrial Enzymology Course Code: BT3452

Duration: 90 min Date: 06/03/2023 Maximum Marks: 50 marks

Instructions:
1. Before attempting any question paper, be sure that you got the correct question paper.
2. The missing data, if any, may be assumed suitably
3. Use the sketches wherever necessary
Ques
tion Question Mark BTL CO
No
Part A (5×2 = 10 Marks) Answer all Questions
1. What are enzymes? 2 L1 CO1

2. What are the general properties of enzymes? 2 L1 CO1

3. Define the term absolute specificity in enzymes. Give examples. 2 L1 CO1

4. Enzymes affect the reaction rates and not equilibria. Justify the statement. 2 L1 CO1

5. Define active site of an enzyme. 2 L2 CO1

Part B (2×13 = 26 Marks)

Write short note on

6. a. (i) Development of enzyme 13 L5 CO1
(ii) Induced-Fit Model.
or
Explain the mechanism of collision theory and transition state theory for enzyme
b. 13 L4 CO1
catalysis in detail.

Explain the following

7. a. (i) Principle of catalysis. 13 L5 CO1
(ii) Role of entropy in catalysis.
or
Explain the terms (i)absolute specificity (ii)group specificity (iii)Prosthetic group
b. 13 L6 CO1
(iv)holoenzyme
Part C (1×14 = 14 Marks)
Explain in detail about the chemical mechanisms in which the use of enzymes it
8. a. 14 L6 CO1
easier to get to the transition state?
or
Discuss about naming and classification of enzymes based on the types of
b. 14 L5 CO1
reaction.

Subject Staff HOD