Professional Documents
Culture Documents
DOI 10.1007/s11947-011-0607-6
1
COMMUNICATION
Received: 31 January 2011 / Accepted: 15 May 2011 / Published online: 25 May 2011
Ⓒ Springer Science+Business Media, LLC 2011
Abstract In the present study, selected engineering and (linseed) is gaining popularity in the food sector for its
biochemical properties of 11 flaxseed varieties namely Sheela, functional properties since it is a leading source of the
Sweta, Garima, Sharda, Rashmi, Shikha, Padmini, omega-3 fatty acid (ALA 52% of the total fatty acids) and
Shekhar, Neelam, LC-2063 and LC-2023, cultivated in
of phenolic compounds known as lignans (>500 μg g−1) ‘as
India, were determined and compared. The average length,
is’ basis. The main components of flaxseed expressed on a
breadth and thickness were found in the range of 4.59 to
moisture-free basis are protein (21%), dietary fibre (28%)
5.73 mm, 2.30 to
and fat (41%) (Bilek and Turhan 2009). So, flaxseed is
2.93 mm and 0.85 to 1.06 mm, respectively. Neelam making great strides in the world’s food supply, and
variety had the highest surface area, geometric mean
demand for human food and live stock markets is expected
diameter, unit volume and 1000-seed mass, whereas LC- to increase owing to the unique properties of this ancient
2023 had the least values among these 11 varieties of
crop (Oomah 2001). The seeds have a crisp and chewy
flaxseed for these properties. The angle of repose varied texture and a pleasant, nutty taste (Carter 1993). Flaxseed
from 19.3 to 23.5°, and the rupture force ranged from
has a storage life of more than 12 months at 9–10% water
30.57 to 46.87 N. The α- linolenic acid content was found content (Coskuner and Karababa 2007a).
from 37.05% to 54.59% (on fat basis) and protein, from The knowledge of engineering properties of agricultural
17.78% to 24.62%. materials is of importance to plant breeders, engineers,
machine manufacturers, food scientists, processors and
Keywords Flaxseed . Physical properties . Colour
consumers (Ozturk et al. 2009). The information on
Frictional properties . Mechanical properties . Nutritional engineering properties of flaxseed is essential to facilitate
composition . Omega-3 and improve the design of equipment for handling, harvest-
ing, processing, transportation, storage, separation and
packing of seed (Selvi et al. 2006). The various properties
Introduction of crop seeds have been studied and reported in the
literature such as guar seed (Vishwakarma et al. 2011),
Flaxseed has been a human food since ancient times
(Vaisey-Genser and Morris 2003). Nowadays, flaxseed
Prosopis africana seed (Akaaimo and Raji 2006), faba bean
K. K. Singh : M. D. : P. Barnwal (*) : J. Rehal grains (Altuntas and Yıldız 2007), makhana (Jha 1999),
Food Grains and Oilseeds Processing Division,
linseed (Selvi et al. 2006), sesame seeds (Tunde-Akintunde
Central Institute of Post Harvest Engineering and Technology,
Ludhiana 141 004 Punjab, India and Akintunde 2004), rice (Shittu et al. 2009), flaxseed
e-mail: psbarnwal@gmail.com (Coskuner and Karababa 2007a), cumin (Singh and
K. K. Singh Goswami 1996; 1998), etc. The varietal comparison of
e-mail: singh_ciae@yahoo.com engineering and biochemical properties of flaxseed is not
M. D. available in published literature.
e-mail: mridulads4@gmail.com In the present study, therefore, the engineering and
J. Rehal biochemical properties of 11 major varieties of flaxseed,
e-mail: kaur_jagbir@yahoo.com grown in India, were investigated to provide useful
Food Bioprocess Technol (2013) 6:598–605
information to engineers to design cleaning, grading, circular plate (Jha 1999; Kingsly et al. 2006; Akaaimo and
drying, conveying, milling and other processing equipment Raji 2006). The static coefficient of friction was
and for food scientists to develop specific food products determined using a laboratory set up according to Jha
for better utilization of flaxseed varieties. (1999) on four structural surfaces, viz. plywood,
galvanized iron, alumin- ium and mild steel sheet which
are common materials used for transportation, storage and
Materials and Methods handling of grains.
Mechanical properties such as rupture force, energy
Raw Material absorbed and deformation of seeds were determined using
Texture Analyser (TA-Hdi, Stable Micro system, UK;
Eleven major varieties of flaxseed grown in India, viz. equipped with a 500-N load cell; graph recorder; test
Sheela, Sweta, Garima, Sharda Rashmi, Shikha, Padmini, speed, 1 mm/s; post test speed, 2 mm/s with a distance of
Shekhar, Neelam, LC-2063 and LC-2023, were procured 30% using SMS P/5 probe). The individual seed was
from CSAUA&T, Kanpur and PAU, Ludhiana during the loaded between probe and base plate and compressed at
year 2009. The engineering and biochemical properties of preset conditions until rupture occurred which is denoted
cleaned seeds were assessed on their ‘as is’ moisture by bio-yield point (N) in the force-deformation curve. The
basis. energy absorbed (N-mm, i.e. mJ) by the seed sample
during loading up to seed rupture was calculated by
Geometrical Properties determining the area under the force-deformation curve up
to the seed rupture point (Altuntas and Yıldız 2007;
To determine the size and shape of flaxseed, length, Kingsly et al. 2006; Singh and Goswami 1998).
breadth and thickness of randomly selected 100 seeds of Deformation (strain) was taken as change in
each variety were measured by using a digital calliper dimensions/original dimensions of the seed.
(Model No. CD-6” CSX, Mitutoyo, Japan; least count,
0.01 mm). The geometric mean diameter (GMD), Biochemical Properties
sphericity, surface area and unit volume of flaxseed were
computed as per standard relationships using values of Moisture, crude fat, protein (using the factor 6.5×N), ash,
length, breadth and thickness (Singh et al. 2004; Mohsenin crude fibre, calcium and iron in flaxseed were determined
1980; Tunde- Akintunde and Akintunde 2004). as per standard methods (AOAC 2000). Total carbohydrate
value was obtained by difference. Total calories were
Gravimetrical Properties calculated by multiplying protein, carbohydrates and fat
content by 4, 4 and 9, respectively. The α-linolenic acid
Bulk density was determined by filling a 250-ml cylinder content was determined using gas liquid chromatography
with seeds from a set height, tapping twice and then by converting fatty acids into methyl/ethyl esters and
weighing the contents (Singh et al. 2004; Mohsenin 1980). comparing with a set of standard esters (Appleqvist 1968).
True density was measured using the toluene displacement
method (Mohsenin 1980). The porosity of seed was Statistical Analysis
calculated from the values of true density and bulk density
using standard relationship (Shittu et al. 2009; Mohsenin Data were analysed as per one-factor analysis of variance
1980). The 1000-seed mass (grams) of randomly selected using LSD of AgRes software statistical package.
seeds was determined using a weighing balance having an
accuracy of 0.001 g.
Results and Discussion
Colour Parameters
Geometrical Properties
To measure colour, L, a and b values of the flaxseed were
determined by Hunter Lab Miniscan XE plus colorimeter. The length, breadth and thickness of the flaxseed varieties
Hue angle (h°) and Chroma (C*) were computed by using varied from 4.59 (var. LC-2023) to 5.73 mm (var.
the standard formula (Hernandez et al. 2008). Neelam),
2.30 (var. LC-2023) to 2.93 mm (var. Neelam) and 0.85
Frictional and Mechanical Properties (var. Sheela) to 1.06 mm (var. Sweta and Padmini),
respectively (Table 1). Selvi et al. (2006) reported the
The angle of repose was calculated from the height and length of linseed in the range of 4.58 to 4.7 mm, and no
diameter of the naturally formed heap of the seeds on a
Food Bioprocess Technol (2013) 6:598– 3
change in the width and thickness for the moisture range
from 8.25% to 11.75% (dry basis (d.b.)). The length,
width and thickness of sesame seed varied from 2.50 to
2.95 mm,
6 Food Bioprocess Technol (2013) 6:598–
Variety Length (mm) Breadth (mm) Thickness (mm) GMD (mm) Sphericity (%) Surface area (mm2) Unit volume (mm3)
Sheela 5.04ef (0.27) 2.47e (0.12) 0.85e (0.12) 3.53g (0.13) 43.58g (2.09) 9.28f (1.42) 5.5e (0.91)
Shweta 5.01f (0.26) 2.50e (0.12) 1.06a (0.16) 4.42de (0.13) 47.17ab (2.81) 12.00d (1.58) 6.9c (1.12)
Garima 4.90g (0.20) 2.60d (0.10) 0.93cd (0.11) 3.96f (0.10) 46.51bc (2.07) 10.67e (1.16) 6.2d (0.80)
Sharda 5.33c (0.27) 2.72c (0.14) 0.92cd (0.12) 4.46cd (0.12) 44.50f (2.23) 11.91cd (1.50) 7.0c (1.05)
Rashmi 5.49b (0.34) 2.59d (0.13) 0.99ab (0.13) 4.74b (0.13) 44.11fg (2.23) 12.68b (1.70) 7.5b (1.19)
Shikha 5.17d (0.35) 2.78b (0.27) 0.95bcd (0.62) 4.54d (0.29) 45.43de (5.70) 12.26cd (4.52) 7.1bc (3.80)
Padmini 5.04ef (0.26) 2.59d (0.12) 1.06a (0.11) 4.60bcd (0.12) 47.58a (1.95) 12.55cd (1.44) 7.2bc (1.00)
Shekhar 5.09de (0.39) 2.72c (0.15) 1.01ab (0.11) 4.67bc (0.13) 47.46a (3.82) 12.73bc (1.63) 7.3bc (1.15)
Neelam 5.73a (0.27) 2.93a (0.12) 1.00ab (0.13) 5.60a (0.14) 44.60ef (1.98) 15.12a (1.90) 8.8a (1.44)
LC-2063 4.61h (0.23) 2.32f (0.10) 0.96bc (0.09) 3.41g (0.08) 47.15ab (2.33) 9.15f (0.94) 5.4e (0.61)
LC-2023 4.59h (0.27) 2.30f (0.11) 0.89de (0.14) 3.12h (0.13) 45.88cd (3.22) 8.26g (1.31) 4.9f (0.84)
F value 142.13*** 179.68*** 8.90*** 81.61*** 24.40*** 74.31*** 55.28***
CD0.05 0.08 0.04 0.06 0.04 0.008 0.57 0.44
CV (%) 5.63 5.51 22.87 6.24 6.50 13.12 22.49
Values in parenthesis are standard deviation; values with different lowercase letters are significantly different at P≤0.05; all values are mean of
100 replicates
***P<0.001
Gravimetrical Properties
of Sheela, Sharda, Rashmi, Shikha and Padmini varieties 2007), fenugreek seed (Altuntas et al. 2005) and coriander
of flaxseed was different (moisture range of 7.02% to seeds (Coşkuner and Karababa 2007b).
9.28%), the bulk density of these varieties was statistically The porosity of the seeds ranged from 34.9% (var. LC-
similar. Coskuner and Karababa (2007a) reported the bulk 2023 and LC-2063) to 41.4% (var. Shikha). The unit
density of 726.6 to 555.6 kg/m3 for the moisture range of volume and 1000-seed mass were in the range of 4.9 to
6.09% to 16.81% (d.b.), whereas Selvi et al. (2006) 8.8 mm3 and 5.7 to 9.8 g, respectively, where the least
observed the bulk density of 690.5 to 545.0 kg/m3 for value was shown by LC-2023 variety and highest value
the moisture range of 6.09% to 16.81% (d.b.) for the was recorded for Neelam variety of flaxseed. The 1000-
commercial variety of linseed. For the studied flaxseed seed mass was found higher in this study than reported by
varieties, the bulk density was observed lower than vetch Selvi et al. (2006), which was 6.0 g at 8.25% (d.b.)
seed (Yalcin and Ozarslan 2004) and higher than sesame moisture content for linseed. This indicated that the
(Tunde-Akintunde and Akintunde 2004), cotton seed varieties grown in India are heavier. The statistical analysis
(Ozarslan 2002), cumin seed (Singh and Goswami indicated that there was a significant difference (P≤0.05)
1996), rapeseed (Calisir et al. 2005), pea (Yalcin et al. among varieties for true density, bulk density, porosity and
Sheela 51.55a (0.73) 5.33f (0.38) 6.72d (0.42) 51.58d (1.01) 8.57d (0.54)
Shweta 49.54bcd (1.54) 6.58cde (0.73) 9.85abc (0.93) 56.27a (0.59) 11.85ab (1.18)
Garima 48.09def (0.57) 7.69a (0.18) 10.20a (0.61) 52.97bc (1.05) 12.77a (0.59)
Sharda 49.70bcd (0.82) 6.85bcd (0.53) 10.03ab (0.73) 55.70a (0.47) 12.15ab (0.89)
Rashmi 50.51abc (1.01) 4.34f (0.15) 5.62d (0.14) 52.34cd (0.69) 7.10d (0.18)
Shikha 50.71abc (0.70) 6.28de (0.34) 9.31bc (0.36) 56.01a (0.39) 11.23bc (0.49)
Padmini 48.84d (0.57) 7.12abc (0.16) 9.77abc (0.31) 53.90b (1.39) 12.09ab (0.18)
Shekhar 47.25f (1.06) 7.32ab (0.27) 9.49abc (0.42) 52.35cd (0.44) 11.99ab (0.49)
Neelam 49.18cd (1.42) 6.31de (0.37) 9.29bc (0.23) 55.86a (0.93) 11.23bc (0.40)
Values in parenthesis are stan-
dard deviation; values with dif- LC-2063 47.56ef (0.92) 5.98e (0.12) 9.06c (0.09) 56.55a (0.67) 10.85c (0.07)
ferent lowercase letters are LC-2023 50.98ab (0.77) 6.31de (0.19) 9.59abc (0.29) 56.63a (0.33) 11.48bc (0.34)
significantly different at P≤ F value 6.46*** 20.56*** 28.03*** 18.22*** 25.96***
0.05; all values are mean of five
replicates. CD0.05 1.64 0.60 0.80 1.34 0.98
***P<0.001 CV (%) 1.96 5.64 5.30 1.45 5.23
6 Food Bioprocess Technol (2013) 6:598–
Sheela 23.5a (0.40) 0.52a (0.003) 0.48a (0.007) 0.46a (0.007) 0.56a (0.007)
Sweta 22.0b (0.35) 0.48de (0.006) 0.42d (.006) 0.39e (0.006) 0.50d (0.006)
Garima 19.3f (0.92) 0.47ef (0.005) 0.41e (0.006) 0.41c (0.006) 0.47e (0.006)
Sharda 20.7de (0.35) 0.47f (0.005) 0.43d (0.006) 0.35f (0.006) 0.45fg (0.006)
Rashmi 20.6de (0.40) 0.51b (0.006) 0.40c (0.006) 0.43b (0.006) 0.50d (0.006)
Shikha 20.0ef (0.35) 0.49c (0.006) 0.43d (0.006) 0.46a (0.006) 0.53b (0.006)
Padmini 22.2b (0.35) 0.49c (0.006) 0.45c (0.006) 0.43b (0.006) 0.51c (0.006)
Shekhar 21.8b (0.35) 0.48cd (0.006) 0.38f (0.006) 0.40d (0.006) 0.45g (0.006)
Neelam 20.6de (0.40) 0.46g (.007) 0.42d (0.006) 0.40e (0.006) 0.51c (0.006)
LC-2063 21.5bc (0.40) 0.48de (0.006) 0.46b (0.007) 0.43b (0.003) 0.46f (0.006)
LC-2023 20.8cd (0.40) 0.44h (0.006) 0.43d (0.006) 0.39e (0.006) 0.45g (0.006)
F value 23.13*** 59.27*** 72.51*** 121.70*** 140.34***
CD0.05 0.70 0.008 0.008 0.008 0.008
CV (%) 1.96 1.25 1.41 1.41 1.23
Values in parenthesis are standard deviation; values with different lowercase letters are significantly different at P≤0.05; all values are mean of
three replicates
***P<0.001
1000-seed mass. Porosity of flaxseed of the studied 11 Shekhar, respectively (Table 3). Lightness was found
varieties was found lower than cumin seed (Singh and statistically similar in Shekhar, Sweta, Garima, Sharda,
Goswami 1996), millet (Baryeh 2002), fenugreek seed Padmini and Neelam varieties of flaxseed. The hue angle
(Altuntas et al. 2005), barnyard millet grain (Singh et al. ranged from 51.58 to 56.63° with least value for var.
2010) and slightly higher than vetch (Yalcin and Ozarslan Sheela and highest for LC-2023 variety of flaxseed, which
2004) and coriander seed (Coşkuner and Karababa 2007b). was lower than new common bean cv. Elkoca-05 (Ozturk
et al. 2009) and new registered common bean cv. Kantar-
Colour Parameters 05 (Ozturk et al. 2010). Chroma values were in the range
of 7.10 to 12.77 with lowest for Rashmi and highest for
The maximum and minimum values for lightness (L) Garima variety of flaxseed, which was lower than
were found as 51.55 for var. Sheela and 47.25 for var. registered common bean cv. Kantar-05 (Ozturk et al.
α-linolenic acid
for new common bean cv. Elkoca-05. Chroma values of
Sweta, Sharda, Shikha, Padmini, Shekhar, Neelam and LC-
47.34d
47.56d
37.05g
46.88e
50.77c
46.85e
50.65c
44.58f
(0.15)
(0.25)
(0.19)
(0.15)
(0.13)
(0.18)
(0.08)
(0.23)
2023 varieties of flaxseed were statistically similar.
9.30bc
9.42bc
10.45a
(0.74)
(0.52)
(0.47)
(0.33)
(0.55)
(0.31)
(0.52)
(0.21)
7.23d
6.91d
5.97e
6.00e
8.70c
The angle of repose of the studied varieties ranged from
Iron
19.3° for var. Garima to 23.5° for var. Sheela (Table 4). In
general, the angle of repose of different varieties was
statistically different (P<0.05), but Sweta, Padmini, Shek-
har and LC-2063 varieties of flaxseed showed statistically
Values in parenthesis are standard deviation; values with different lowercase letters are significantly different at P≤0.05; all values are mean of three
118.26bc
113.42cd
Calcium
120.46b
120.97b
102.72e
98.46e
98.51e
90.77f
similar angle of repose. Almost similar results for angle of
(2.15)
(3.30)
(1.79)
(1.09)
(2.17)
(4.29)
(3.36)
(3.80)
repose have also been reported by Coskuner and Karababa
(2007a) and Selvi et al. (2006), who reported an angle of
repose of 21.6° at 6.09% and 21.59° at 8.25% (d.b.)
561.21de
569.37bc
565.23cd
556.95ef
moisture content, respectively. The angle of repose of
Calories
547.66g
573.54b
587.30a
590.72a
(5.46)
(0.78)
(2.56)
(1.54)
(2.04)
(2.03)
(2.74)
(1.39)
flaxseed was found higher than fenugreek seed (Altuntas et
al. 2005), faba bean grain (Altuntas and Yıldız 2007) and
lower than sesame seed (Tunde-Akintunde and Akintunde
Carbohydrate
2004), cumin seed (Singh and Goswami 1996) and millet
(Baryeh 2002).
(2.22)
(0.80)
(0.98)
(0.50)
(0.63)
(0.71)
(0.30)
(0.51)
27.12
24.56
29.67
23.76
23.73
25.48
24.81
22.92
The static coefficient of friction was least for galvanized
iron among the four studied surfaces, which may be due to
the smoother surface of the galvanized iron compared to
Crude fibre
6.61de
6.29ef
(0.20)
(0.26)
(0.15)
(0.25)
(0.02)
(0.27)
(0.06)
(0.17)
7.36b
6.77d
0.44 to 0.52, 0.38 to 0.48, 0.35 to 0.46 and 0.45 to 0.56 for
8.14a
6.12f
7.8a
plywood, aluminium, galvanized iron and mild steel sheet,
respectively (Table 4). Coskuner and Karababa (2007a)
also reported the comparable values of static coefficient of
Crude fibre, fat-
free basis (%)
11.28bc
10.17ef
13.29a
12.84a
(0.18)
(0.37)
(0.36)
(0.32)
(0.07)
(0.15)
(0.36)
(0.33)
6.09%. Flaxseed var. Sheela showed the highest static
coefficient of friction for all the four surfaces, while var.
Sharda showed the least value for galvanized iron and mild
steel surfaces. Flaxseed varieties Sharda, Shekhar and LC-
4.07bc
(0.42)
(0.34)
(0.16)
(0.10)
(0.05)
(0.14)
(0.16)
(0.08)
3.24d
3.88c
3.90c
3.86c
3.89c
4.53a
3.91c
Ash
39.91de
43.38b
43.23b
46.44a
42.15c
46.56a
38.51f
(0.88)
(0.27)
(0.39)
(0.29)
(0.41)
(0.19)
(0.40)
(0.39)
than faba bean grain (Altuntas and Yıldız 2007) and vetch
seed (Yalcin and Ozarslan 2004), whereas in the case of
17.78h (0.09)
19.99g (0.05)
24.62a (0.44)
22.02cde
21.35def
21.03efg
20.60fg
Protein
(0.68)
(0.33)
(0.26)
(0.56)
(0.58)
ns nonsignificant
(0.02)
(0.44)
(0.03)
(0.03)
(0.05)
(0.04)
(0.07)
7.02g
9.28b
8.92d
6.86g
8.36e
8.25e
7.56c
8.65f
Padm
Sheel
Shwe
Rash
Shik
Shar
Gari
Var
ma
mi
ini
da
ha