Professional Documents
Culture Documents
CHEMISTRY
By
(SC20A819)
Faculty of Science of the University of Buea in Partial Fulfilment of the Requirements for the
Supervisor:
Dr TOH BOYO
JUNE 2023
0
DEDICATION
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UNIVERSITY OF BUEA
CERTIFICATION
This is to certify that the work entitled: “Soil analysis in the university of Buea around dumped
areas and five hundred meters away around non-dumped areas to determine phisico-
chemical parameters of the soil” By TUM ELLIOT WANYI (SC20A819) submitted to the
department of chemistry in partial fulfilment of the requirements for the award of a bachelor of
Date:
DR TOH BOYO
Lecturer
(Supervisor)
ii
ACKNOWLEDGEMENT
I would like to express my special thanks to my supervisor Dr Toh Boyo for his guardians and
support in completing my project. A special thanks and gratitude my family and friends for their
support.
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ABSTRACT
Soil is the most diverse rich nutrient rich loose surface material which covers most of our land.
Soil contains both the inorganic and organic matter. The presence of soil is necessary to prevent
direct leaching of the inorganic(mineral) particles in the ground water. Color texture structure
porosity density consistency temperature air are the physical properties of soil. Suitability of soil
for agriculture is determined by the physical properties. There are different properties for different
soils. Soil is the source of water and provide mechanical stability to the plants. There are various
types of soil in the environment for example loam soil, sandy soil, chalk, peat, silt, clay soil. Soil
analysis refers to a set of various chemical processes which help us determine the available plant
nutrient which are either in micronutrients of macronutrients form. I this study various
physicochemical parameters (PH, electrical conductivity, soil moisture, soil organic matter) were
monitored, after carrying out this analysis, we understood that the properties of soil keep changing
depending on various anthropological activities carried out nearby. The range in which values
keeps fluctuating, sometimes under range, sometimes totally beyond it. Through such studies we
endeavor to highlight the potential for hazardous impact that nature might face due to excessive
anthropological activity.
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TABLE OF CONTENT
DEDICATION.............................................................................................................................................. i
CERTIFICATION ...................................................................................................................................... ii
ABSTRACT ................................................................................................................................................ iv
v
3.4 DETERMINATION OF SOIL CONDUCTIVITY ............................................................................ 9
REFERENCE ............................................................................................................................................ 16
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CHAPTER 1: INTRODUCTION
mineral organic matter and microbial community of soil (Lawry et al, 1951) the discharge of
industrial effluents especially without treatment may have profound influence on physio-chemical
and biological properties of the soil (David shyam babu, 2010). A major industry that produces
huge volume of waste water contains several toxic and non-degradable organic materials, may
affect soil enzyme activity, which in turn affect soil fertility. In reality the soil enzymes play a vital
role in catalyzing reactions associated with organic matter decomposition and nutrient recycling
(oonkothai and parvatham, 2005). Also organic matter equally plays an important role in
enzymatic activity. The fertility of the soil in natural agroecosystem depends on the microbial
(Joniec, 2018). The important parameters which reflect soil quality are; quantity and quality of
organic matter, soil structure, PH, conductivity, moisture content amongst others. Soil physico-
chemical parameters are basic indicators for estimating the level of nutrient content and
characteristics. It was observed that available nutrients balance in soil was influenced by soil PH.
Soil electrical conductivity can serve as a measurement of soluble nutrients and it is useful in
monitoring the mineralization of organic matter in soil (ingole, 2015; sde et al, 2000). Additionally,
soil enzyme activities are commonly influenced by soil PH and enzyme activities also has control
on SOC (soil organic carbon), (Bueis et al, 2018) in general soil physico-chemical properties and
soil enzymes do great effort on SOC dynamics together. The physico-chemical study of soil is
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very important and this knowledge will help us understand the status of soil and also to the people
The soil on university grounds plays a crucial role in the growth of plants and overall
environmental health. However, due to the continuous use and exposure to various environmental
factors, the quality of the soil may deteriorate over time. It is important to regularly monitor the
physicochemical properties of the soil to ensure that it remains suitable for plant growth and
environmental sustainability.
Therefore, the university needs to establish a soil analysis program to determine the
physicochemical properties of the soil on its campus. The program should include regular sampling
and testing of soil from different locations on campus to assess the pH, organic matter content,
The results of the soil analysis can be used to develop strategies to improve the quality of the soil
if necessary, such as by adding organic matter or adjusting the pH. Additionally, this data can be
used to inform decisions about plant selection and management practices that are best suited to the
Overall, the establishment of a comprehensive soil analysis program will ensure that the university
grounds remain healthy and sustainable for both plants and people
1.2 RATIONAL
The dump from local community’s industries laboratories amongst others affect the soil negatively
as it destabilizes the soil by contaminating it with the chemical impurities killing microorganisms
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like bacteria which play an important role in humus formation. This limitation may affect both
crops and living organisms present around the area, so it is very important to carry out soil analysis
to determine the physiochemical parameters around the area and carry out conclusions and
1.3 OBJECTIVES
To evaluate the fertility and nutrient status of soil in the university of buea, around dumped sites
and 500m away around non dumped sites by determining some physical parameters such as soil
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CHATER 2: LITERARATURE REVIEW
Soil physicochemical properties play a crucial role in determining soil quality and fertility, and
environmental settings. Here are some relevant literature references on the physicochemical
determination of soil in a university context. Soil is the biologically active and porous medium that
has developed in the uppermost layes of earth’s crust. It serves as a reservoir of water and nutrients
and a medium for the filtration and breakdown of injurious wastes. Soil is the loose surface
material that covers most land. It consists of organic particles and organic matter. soil provides the
structural support to plants used in agriculture and is also their source of water and nutrients soil
vary greatly in their chemical and physical properties. There are four main types of soil that is
sandy soil, clay or loam soil and silt soil, with others like alluvial soil, black cotton soil, red and
yellow soil, laterite soil, mountainous or forest soil and desert soil, saline and alkaline soil, peaty
and marshy soil. The four main types which are sandy, silt, clay and loam all got uses. The sandy
soil is a type which is dry, light and gritty and does not hold water well. Most sandy soils are acidic
and lack a lot of nutrients. Because this soil drains water quickly, this is one of the best types of
soil to grow vegetables because of the good drainage and because it warms up quickly. However,
it doesn’t retain nutrients throughout the growing season adding compost or manure for example
is an excellent way to enhance sandy soil. Silt soil is parked with nutrients as it originates from
river sediments. It’s one of the best types of soil for gardening as a lot of plants flourish in silt. The
particles are medium sized therefore retain water quite well but drainage can become a problem.
An issue with silt soil is that it is easily compacted, this is because a problem with downpour of
rain that can wash it away or if its continually walked on. Adding organic matter to create larger
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Clay soils can be very difficult for inexperienced gardeners. It’s very heavy and holds a lot of
nutrients which is good. However, it also holds a high amount of water and drains very slowly.
This means that it remains very moist and wet in winter and dries out and cracks in summer. Clay
soil is useful for growing crops such as wheat but needs to be amended with organic matter. Loam
soil is a mixture of the three previous different types of soil, sand, silt and clay. The combination
works to create fertile, rich soil that offsets the negative characteristics of the three soil on their
own. There is always a dominant component that can make this soil a sandy loam or clay loam.
This is actually on of the best types of soils for plants and gardening and experienced gardeners
and beginners alike will love working with it. The mixture of particles holds a lot of nutrients and
has excellent drainage the perfect condition for growing the soil is often referred to as agricultural
Soil contamination occurs when human made chemicals, such as hydrocarbons and heavy metals
find their way into the earth altering the natural soil environment. Generally, soil contamination is
cited as a consequence of non-organic farming particles, industrial activity and improper waste
leads, solvents, pesticides and poly-nuclear aromatic hydrocarbons such as naphthalene. Pesticides
and insecticides which are designated to destroy pest and insects and herbicides which are
designated to kill weeds are used by farmers in order to protect their crops. Synthetic pesticides,
insecticides and herbicides are created in the laboratory using a combination of chemicals. In the
past the use of synthetic pesticides, insecticides and herbicides has resulted in soil contamination.
Improper waste disposal has also lead to soil contamination. Treated sewage, also known as bio
solids, is sometimes used as a fertilizer. Higher in contaminants than other fertilizer, the use of bio
solid fertilizers ought to be subject to greater regulation. In addition, munitions may also cause soil
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contamination, the chemicals present in biological weapons and other types of weapons can leach
into soil, greatly upsetting its natural balance. Soil pollution causes a chain of reactions, it alters
soil biodiversity, reduces soil organic matter and soils capability to act as a filter. It also
contaminates the water stored in the soil and ground water and causes an imbalance of soil
nutrients. Among the most common soil pollutants are heavy metals, persistent organic pollutants
and emerging pollutants like pharmaceuticals and personal care products. (Brady, N.C. et al 2016).
The nature and properties of soils. Pearson Publishers. This comprehensive textbook provides an
overview of the physical, chemical, and biological properties of soils and their measurement
methods (Clark, R.B. 2001) Understanding soil acidity: A handbook for consultants, growers, and
agricultural professionals. CRC Press. This book focuses on the measurement and management of
soil acidity, which is an important soil property that affects plant growth and nutrient availability
(Gee, G.W., & Bauder, J.W. 1986). Particle-size analysis. In A. Klute (Ed.), Methods of Soil
Analysis: Part 1Physical and Mineralogical Methods (2nd ed., pp. 383-411). American Society of
Agronomy. This chapter describes the particle size analysis method, which is used to determine
the distribution of particle sizes in a soil sample. (Nelson, D.W., & Sommers, L.E. 1996). Total
carbon, organic carbon, and organic matter. In D.L. Sparks et al. (Eds.), Methods of Soil Analysis:
Part 3—Chemical Methods (pp. 961-1010). American Society of Agronomy. This chapter presents
methods for measuring total carbon, organic carbon, and organic matter content in soils (Olsen,
S.R., & Sommers, L.E. 1982). Phosphorus. In A.L. Page et al. (Eds.), Methods of Soil Analysis:
Part 2—Chemical microbiological Properties (2nd ed., pp. 403-430). American Society of
Agronomy. This chapter describes the methods for measuring phosphorus content in soils, which
is an important nutrient for plant growth (Rhoades, J.D. 1996). Salinity: Electrical conductivity
and total dissolved solids. In D.L. Sparks et al. (Eds.), Methods of Soil Analysis: Part 3—Chemical
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Methods (pp. 417-435). American Society of Agronomy. This chapter presents methods for
measuring soil salinity using electrical conductivity and total dissolved solids (Soil Science
Society of America 2017). Glossary of soil science terms. Soil Science Society of America.
7
CHAPTER 3: MATERIALS AND METHODS
Walked across the field in a “W” pattern aiming to collect the sample with the sampling tool
Used a towel to remove a slice of soil about 2.5cm wide ton sample depth and collected in a clean
plastic paper. Furthermore, slices were taken in the same way from different parts of the field until
Four crucibles were dried in the oven and weight recorded. 40g of soil sample was placed in the
crucible and weight of sample and crucible determined and recorded. the crucible with soil content
was then placed in an oven to about 1500C – 2000C for some hours After drying the crucible with
its content was removed from the oven and allowed to cool
The weight of the crucible and its content was determined and recorded after cooling. The moisture
W2−W1
Moisture factor MF = 𝑊3−𝑊1
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3.3 DETERMINATION OF PH
20g of each soil sample was weighed and put in a 100ml beaker 25ml of distilled water was added
to the sample. The suspension was left for 30 minutes with occasional stiring using glass rod in
order to enable it reach equilibrium. The suspension was determined using a PH meter.
20g of each sample was weighed and put into a 100ml beaker 25ml of distilled water was added
to the sample the suspension was left for 30 minutes with occasional stirring using glass rod in
order to enable it reach equilibrium. the conductivity of the suspension was determined using a
conductivity meter
The mass of four emptied and cleaned crucibles was recorded and determined (MP). Part of the
entire oven-dried test from moisture content experiment was placed in the crucible and soil
specimen was determined and recorded(MOPS). The crucible containing the soil specimen was
placed in an oven and temperature gradually increased to about 6000C and left overnight. The
crucible was removed carefully using tongs since it was very hot and allowed to cool and the mass
MO
The organic matter content % was calculated using OM% = 𝑀𝐷 × 100
Where; MO = MD – MA,
MD = MPPS – MP,
MA = MPA – MP
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CHAPTER 4: RESULTS
The physiochemical analysis of the soil samples includes: PH, organic matter, moisture content,
To carry out a statistical comparison, we use the t-test, assuming that S1 and S2 are the same,
Calculation
2 (∑ 𝑥𝑖)2
[ 𝑋1−𝑋2] 𝑛1𝑛2 ∑ 𝑥𝑖 −
t= √ , s=√ 𝑛
,
𝑠𝑝𝑜𝑜𝑙𝑒𝑑 𝑛1+𝑛2 𝑛−1
𝑆 2 1 (𝑛1−1)+𝑆 2 2(𝑛2−1)
spooled = √ = degree of freedom
( 𝑛1+𝑛2−2)
4.2 PH STATISTICS
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Spooled value = 0.1394
t= calculated = 5.344
Degree of freedom = (n1 + n2 – 2) = 2
t-table at 95% C.I = 4.303
t= calculated = 7.682
Degree of freedom = 2
t-table at 95% C.I = 4.303
t= calculated = 0.349
t-table at 95% C.I = 4.303
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Spooled value = 0.0418
t= calculated = 1.645
t-table at 95% C.I = 4.303
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CHAPTER 5: DISSCUSSION, CONCLUSION AND RECOMMENDATION
5.1 DISCUSIONS
Soil parameters play a crucial role in determining the productivity and fertility of soil. In a
various physical and chemical properties of soil samples collected from different locations.
Physical parameters of soil that are commonly analyzed in a university setting include soil texture,
bulk density, porosity, water holding capacity, and soil structure. Soil texture refers to the
proportion of sand, silt, and clay particles in soil samples and is determined using methods such
as the hydrometer method or the laser diffraction method. Bulk density, which is the mass of dry
soil per unit volume, is commonly determined using the core sampling method. Porosity, which is
the proportion of pore spaces in soil, can be determined using the water displacement method or
the mercury intrusion porosimetry method. Water holding capacity, which is the amount of water
that soil can hold, is determined using the pressure plate method.
Chemical parameters of soil that are commonly analyzed in a university setting include soil pH,
electrical conductivity, organic matter content, and nutrient levels. Soil pH refers to the acidity or
conductivity, which is a measure of the ability of soil to conduct electrical current, is commonly
determined using the saturation extract method. Organic matter content, which is the amount of
decomposed organic material in soil, can be determined using the Walkley-Black method or the
loss-on-ignition method. Nutrient levels such as nitrogen, phosphorus, and potassium are
commonly determined using methods such as the Kjeldahl method or the inductively coupled
plasma method.
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Various instruments and equipment are used to carry out physico-chemical analysis of soils in a
5.2 CONCLUSION
The physiochemical studies of parameters are very important to a chemist for plant growth and
soil management. From results above we see the non-dumped sites have a favorable PH ranging
from 5.67 – 5.57 a favorable conductivity which is not too high ranging from 0.79 – 0.92, a high
percentage of organic matter ranging from 18.28% - 17.7 % a good moisture content ranging from
1.098 – 1.1055 indicating better favorable conditions than the dump sites which have a PH from
6.43 – 6.30, low conductivity ranging from 0.25 – 0.26, low organic matter percentage from
16.97% -17.5%, low moisture content 1.0872 – 1.0819, which equally indicates that dumping dirt’s
distorts the management, stability and properties of the soil and if care is not taken properly may
5.3 RECOMMENDATION
Soil analysis is an important tool for determining the physicochemical properties of soil, which
are crucial for plant growth and health. Here are some common physicochemical parameters that
1. pH: Soil pH is a measure of the acidity or alkalinity of the soil and is an important factor in
2. Organic matter content: Organic matter in soil provides nutrients for plant growth, improves
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3. Nutrient content: Soil nutrient content, including nitrogen, phosphorus, potassium, calcium,
magnesium, sulfur, and micronutrients, is important for plant growth and productivity.
4. Cation exchange capacity (CEC): CEC is a measure of the soil's ability to hold onto positively
charged ions, such as calcium, magnesium, and potassium, which are important for plant growth.
5. Electrical conductivity: Electrical conductivity measures the soil's ability to conduct an electrical
6. Texture: Soil texture refers to the relative proportions of sand, silt, and clay particles in the soil
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REFERENCE
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activities and physiochemical properties in the agricultural soil. Appl, soil Ecol. 18.225-
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Bollag J.M, J. Berthelin, D Adriano and Huang P.M (2002). Impact of soil minerals or
Bueis, T, Tumon, M.B. Bravo, F., Pando, v and Muscolo A (2018). Factors determining
enzyme activities in soil under pinus halepensis and pinus sylvestris plantations in Spain.
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Chandral L.R, Gupta S, ande V and Singh characteristics A correlation between soil
David Shyam Babu S (2010) Protease and phosphatase activity of soil contaminated with
diary waste water. Asia J. Microbiol Biotech Enxiron Sci 12(2). 1-5
Hartman W.H, Richard son, C.J Vilgalys, R and Bruland, G.L (2008). Environmental
and Anthropogenic controls over bacterial communities in wetland soils. Proc nath Acad
Ingole, S.P. (2005) A review on role of physiochemical properties in soil quality. Int.
16
Jackson M.L (1973) soil chemical analysis. Pentice Hall of Indian pvt. Ltd New Delhi,
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Jobbagy, E.G, and Jackson, R.B (2000). The vertical distribution of soil organic carbon
soil reclaimed woth various types of was tint J Environ Sci Technol 15:2241-2252
Kaushik A, Nisha R, Jagjecta K and Kaushik C.P (2005) impact of long and short term
Lopedota, o., leogrande, R., Fiore, A., Debiase, G and Montemuro, F (2013) Yield and
soil responses of melon grown with different organic fertilizers J plant Nutri 36(3): 415-
428
Lowry, O.H, Rouse Bough, H.I, Fair, A.L and Randall, R.I (1951) protein measurement
Brady, N.C., & Weil, R.R. (2016). The nature and properties of soils. Pearson
Clark, R.B. (2001) Understanding soil acidity: A handbook for consultants, growers,
and agricultural professionals. CRC Press. This book focuses on the measurement and
management of soil acidity, which is an important soil property that affects plant growth
Gee, G.W., & Bauder, J.W. (1986). Particle-size analysis. In A. Klute (Ed.), Methods of
Soil Analysis: Part 1—Physical and Mineralogical Methods (2nd ed., pp. 383-411).
17
American Society of Agronomy. This chapter describes the particle size analysis
method, which is used to determine the distribution of particle sizes in a soil sample.
Nelson, D.W., & Sommers, L.E. (1996). Total carbon, organic carbon, and organic
matter. In D.L. Sparks et al. (Eds.), Methods of Soil Analysis: Part 3—Chemical
methods for measuring total carbon, organic carbon, and organic matter content in soils.
Olsen, S.R., & Sommers, L.E. (1982). Phosphorus. In A.L. Page et al. (Eds.), Methods of
Soil Analysis: Part 2—Chemical and Microbiological Properties (2nd ed., pp. 403-430).
American Society of Agronomy. This chapter describes the methods for measuring
Rhoades, J.D. (1996). Salinity: Electrical conductivity and total dissolved solids. In D.L.
Sparks et al. (Eds.), Methods of Soil Analysis: Part 3—Chemical Methods (pp. 417-
435). American Society of Agronomy. This chapter presents methods for measuring soil
7. Soil Science Society of America (2017). Glossary of soil science terms. Soil Science
Society of America.
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