1.

Human insulin formed by recombinant DNA technology is known as

A. H insulin

B. R insulin

C. humulin

D. huinsulin

Answer & Explanation

Answer: Option C

Explanation:

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2. The gene coding for VP1 is cloned in

A. pMB 9

B. pBR 322

C. pUC 18

D. pUC 19

Answer & Explanation

Answer: Option B

Explanation:

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3. How many nucleotides are there in Ss RNA molecule of picorna virus causing FMD?

A. 1000 B. 5000

C. 8000 D. 10000

Answer & Explanation

 Answer: Option C

Explanation:

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4. Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following
antigen?

A. Viral core antigen (HBcAg)

B. Viral surface antigen (HBsAg)

C. e antigen (HBeAg)

D. All of the above

Answer & Explanation

Answer: Option B

Explanation:

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5. Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid
pBR 322 by the side of

A. β-Galactosidase

B. galactokinase

C. acid phosphatase

D. glucokinase

Answer & Explanation

Answer: Option A
6. Human growth hormone (hGH) is secreted by

A. pitutary gland
 B. hypothalamus

C. pancreas

D. none of these

Answer & Explanation

Answer: Option A

Explanation:

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7. The promoter used for cloning HBV surface antigen is

A. galactokinase

B. alcohol dehydrogenase 1

C. β-galactosidase

D. polyhedrin

Answer & Explanation

Answer: Option B

Explanation:

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8. Recombinant vaccine for HBV was produced by cloning viral genes in

A. plasmids

B. cosmids

C. autonomously replicating plasmid of yeast

D. phagemids

Answer & Explanation

 Answer: Option C

Explanation:

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9. The first therapeutic product formed by means of recombinant DNA technology is

A. human growth hormone

B. insulin

C. hepatitis B vaccine

D. vaccine for foot and mouth disease

Answer & Explanation

Answer: Option B

Explanation:

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10. Which one of the following capsid protein of picorna virus is immunogenic in nature?

A. VP1 B. VP2

C. VP3 D. VP4

Answer & Explanation

Answer: Option A
11. The two chains are joined together to constitute native insulin using

A. sodium dissulphonate and sodium sulphite

B. sodium dissulphonate and sodium sulphate

C. sodium sulphate and sodium sulphite

D. sodium sulphate only

Answer & Explanation

 Answer: Option A

Explanation:

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12. Foot and mouth disease (FMD) of animals is caused by

A. RNA virus

B. DNA virus

C. bacteria

D. protozoa

Answer & Explanation

Answer: Option A

Explanation:

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13. In India first genetically engineered vaccine against HBV was developed by

A. Ranbaxy Pvt Ltd

B. Shantha Biotechnics Pvt Ltd

C. Dabur Pvt Ltd

D. Glaxo Pvt Ltd

Answer & Explanation

Answer: Option B

Explanation:

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14. The recombinant hGH lacks

A. terminal glutamine

B. terminal lysine

C. terminal methionine

D. terminal arginine

Answer & Explanation

Answer: Option C

Explanation:

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15. How is human insulin synthesized using recombinant DNA technology?

A. By using chemically synthesized DNA sequences for the two chains separately

B. By isolating DNA from the islets of Langerhans of pancreas

C. By using cDNA for insulin

D. By using chemically synthesized DNA sequences for the complete insulin protein

Answer & Explanation

Answer: Option A
16. The two chains of insulin can be separated by detaching it from β-galactosidase using

A. hydrogen chloride

B. cyanogen bromide

C. peptidase

D. protease

Answer & Explanation

Answer: Option