CLS 422 Clinical Immunohematology I

Take Home Quiz

Day Three

Working independently, select the best answer to the following questions. Submit your
answers via BlackBoard [Clinical Immunohematology I, Assignments, Student Lab,
ABO Discrepancies Module, Quiz Three] before 8am of Day Four.

1. T F Red cell membrane rigidity is due the loss of ATP to drive the
cationic pump.

2. The storage lesion includes a decrease in the following:

a. Glucose
b. pH
c. 2,3-DPG
d. ATP
e. All of the above

3. According to FDA regulations, what percentage of transfused cells must remain in

circulation for 24 hours after transfusion?

a. 50%
b. 65-80%
c. 70-75%
d. 95%

4. The preservative(s) that provide the longest storage period for packed red blood
cells is: (More than one answer may be correct)

a. ACD
b. CPD
c. CP2D
d. CPDA-1
e. AS-1
f. AS-3
g. AS-5
h. heparin

CLS 422 Clinical Immunohematology I Page 1 of 3

Student Lab Day Three Take Home Quiz
5. Match the blood component in column A with the indication for use in column B.
Each answer may be used only once.

Column A Column B

_____ Packed Red Cells A. Decreased platelet numbers or

function

_____ Platelets B. Restore multiple coagulation factors

_____ Fresh Frozen Plasma C. Improve oxygen carrying capacity

_____Cryoprecipitate D. Provide fibrinogen, factor VIII, and

von Willebrand Factor

6. The proper temperature for red cell storage is:

a. < - 18oC
b. 1-6 oC
c. 20-24 oC
d. 36-38 oC

7. Which of the following products is acceptable for routine transfusion to an A

Negative patient? (more than one answer may be correct)

a. O Positive Packed Red Cells

b. A Positive Packed Red Cells
c. O Negative Packed Red Cells
d. AB Positive Plasma
e. A Negative Platelets, Pheresis

8. What is the appropriate concentration for cell suspensions when performing ABO
determinations by tube method?

a. 0.8%
b. 3%
c. 10%
d. 12%

9. How would centrifuging for 45 seconds at 3500rpm affect the results of the ABO
forward grouping?

a. Acceptable spin time, no affect.

b. False negative, cells hemolyzed.
c. False positive, non-immunologic aggregation of cells.
d. False positive, rouleaux induced.

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Student Lab Day Three Take Home Quiz
10. Which of the following is the most common ABO discrepancy?

a. Weak or missing reactions in the reverse grouping.

b. Rouleaux
c. Subgroups of A
d. Acquired B

11. What is usually indicated by mixed field reactions?

a. There has been a clerical error.

b. Incompatible blood has been transfused.
c. Two cell populations are present in the specimen.
d. The patient has elevated levels of serum protein.

12. A discrepancy due to a cold autoantibody may be eliminated by:

a. Washing the patient’s cells with warm saline.

b. Warming the patient’s serum and the reverse grouping cells to 37oC before
testing.
c. Absorbing the patient’s serum with autologous cells.
d. All of the above

13. T F The first step in resolving an ABO discrepancy is to review all

paperwork and labeling.

14. The following results were obtained on a 72 year old male cancer patient:

Reagent: Anti-A Anti-B Anti-D A1 Cells B Cells

Pt results: 2+ 0 3+ 0 0
Screen Cells
I-0 II-0 III-0

No errors in paperwork or labeling were detected. Repeat testing yielded

identical results. What is the next step you would take to resolve this
discrepancy?

a. Wash cells with warm saline.

b. Repeat forward grouping using acidified anti-B.
c. Incubate the reverse grouping at room temperature for 15 minutes.
d. Repeat the D testing using a monoclonal reagent.

CLS 422 Clinical Immunohematology I Page 3 of 3

Student Lab Day Three Take Home Quiz