MACCONKEY AGAR

IDENTIFICATION Solidplate medium, Reddish brown colour,

Transluscent
CONSTITUENTS Peptone, Lactose, Bile salt - selective agent,
Agar, Neutral red (2%) indicator, Distilled water,
pH- 7.4
STERILISATION Autoclaving at 121°Cx 15 mins.
PRINCIPLE Lactose
Bacterial fermentation
Acid
Neutral red Pink colour
RESULT Pink colony - Lactose fermenter
Pale or colourless colony- Lactose nonfermenter.
TYPE OF MEDIUM Selective /Differential / Indicator
USES Primary selective medium for Enterobacteriaceae.
 CYSTEINE LACTOSE ELECTROLYTE
DEFICIENT ( CLED) MEDIUM
IDENTIFICATION - SOLID PLATEDMEDIUM
TRANSLUSCENT
LIGHT COLOUR
INGREDIENTS PEPTONE,TRYPTONE,LAB LEMCO,
L-CYSTEINE,BROMOTHYMOL
BLUE,AGAR,WATER

INDICATOR USED -BROMOTHYMOL BLUE

STERILISATION - AUTOCLAVING AT 121°c
FOR 15 MINS.

TYPE OF MEDIUM DIFFERENTIAL MEDIUM

USES DIFFERENTIATES LACTOSE FERMENTING &

NON FERMENTING COLONIES.
2INHIBITS SWARMING OF PROTEUS AS
ELECTROLYTE IS'DEFICIENT.
SPECIFICALLY USED FOR ISOLATION OF
URINARY PATHOGENS.
SUPPORTS THE GROWTH OF
STAPHYLOCOCCI,STREPTOCOCCI, CANDIDA
THAT FAIL TO GROWIN MACCONKEY.
 MILK AGAR
Identification : Solid plate medium
White coloured opaque

Constituents : Sterile fresh milk added to molten sterile

nutrient
agar at 56 0 C.
Type of medium - Enriched medium
Uses Toenhance pigment production of
Staphylococci
Golden yellow -Staph . aureus
White - Staph. albus
Lemon yellow - Staph. citreus
Modification : Salt milk agar (7- !0% ) selective medium for
Staphylococcus aureus
 14 98 00078 433HS
8202-Ay-2-dX3 6180UdM-ON I07 LO8aM

CHOCOLATE AGAR
(Syn - HEATED BLOOD AGAR)

IDENTIFICATION: Solid phase medium

Chocolate coloured
Opaque

PREPARATION :Sterilised _olid blood agar heated to 75°c

till chocolate brown.

USES : Isolation of fastidious organisms like

-H,influenzae
-Neisseriae
-Stepto .pneumonae
 EEP
RLODD3s EXP-03-JUL -2028
BLOOD AGAR
IDENTIFICATION : Solid plate medium, Blood red colour,
Opaque
CONSTITUENTS Nutrient agar, Sterile sheep blood 5% -10%
(Horse, Rabbit, Human blood may be used),
pH- 7.2 --7.4
STERILISATION Autoclaving Nutrient agar at 121°Cx 15 mins. 15lbs
Cooled to 55°C and blood added aseptically.
TYPE OF MEDIUM Enriched medium and Differential medium (Haemolysis)
USES *Primary isolation medium for Staphylococci., Streptococci & Yeasts.
*Growth of fastidious organisms.
* Haemolysis pattern observed.
*Swarming of Proteus
* Sensitivity testing of Streptococci.
MODIFICATIONS : Selective Blood agar -Addition of Antibiotics.
Potassium tellurite blood agar- C. diphtheriae (Black colonies)
Hard Blood agar ( 6% agar)- Prevents swarming of Proteus.
 SABOURAUD'S DEXTROSE AGAR (SDA)
IDENTIFICATION Read label
Straw yellowcoloured slant in tube with label SDA
CONSTITUENTS Dextrose (4%) - High glucose content,
Peptone, Agar, Distilled water, pH-5.6
STERILISATION Autoclaving at 121°C x 15 mins.

TYPEOF MEDIUM Selective medium

æSES Primary isolation medium for fungi.

MODIFICATIONS * SDA with antibiotics,
* Mycosel agar (SDA + Antibiotics + Cycloheximide)
Cycloheximide prevents growth of saprophytic fungi.
 ROBERTSON'S COOKED MEAT BROTH
IDENTIFICATION Meat particles at the bottom,
Pale yellowish brown liquid.

CONSTITUENTS Chopped & cooked meat (fat free)

Peptone Infusion Broth, pH -7.5
STERILISATION Autoclaving at 121°C x 15 mins.
USES * Cultivation of anaerobes.
* Preservation of stock cultures of aerobic organisms.
Recovèry medium for spores.
Saccharolytic and Proteolytic activity of
Clostridium specie
MODIFICATIONS Salt cooked meat broth- Enrichment medium
for Staphylococcus.
 GLUCOSE BROTH

IDENTIFICATION: Straw coloured Broth (50 ml)

Big (4 0z.) medical flat bottle (screw capped)

CONSTITUENTS: Meat extract broth

Sodium chloride
Peptone
Glucose (1%)
Anticoagulant-Citrate / Liquoid (Sodium polyanethol sulphonate)
STERILISATION: - Autoclving at 116°C 10 lbs. x 10 minutes
-Filtration
-Tyndallisation
USES: * Blood culture
* Promotes luxuriant growth of bacteria
* Anaerobic medium (glucose as reducing agent)
 CASTANEDA'S
MEDIUM
BIPHASIC
IDENTIFICATION: mlrmedical bottle having solid slant
100 flat
&liquid medium(Biphasic)

CONSTITUENTS :Solid phase-St erile serum glucose

phase-Sterile serum
glucose agar
broth

STERILISATION :Sterile
Autoclabroth
ving added110c min
at x 10
after slant
USES :Primary preparation
isolation media for Bråcella
& Salmonella.
Cultivation of
bacteria blood for isolation of slow growing
ADVANTAGES Allows
the bottle.subculture on solid
phase without
Avoids opening
subcultucont
ring.amination associated with frequent
 NUTRIENT AGAR

IDENTIFICATION Solid plate Medium

Straw Yellow coloured
Transluscent
COMPOSITION Peptone, Beaf Extract,
Agar, Distilled water
pH-6.8
TYPEOF MEDIUM Basal solid Medium
USES Basal medium for other media
For Antimierobial Susceptibility Testing
Pigments better observed
Colonies for Antisera agglutination test
For Catâlase test.
In semi solid state : Preservation of bacteria
Holding medium for transport
Motility testing
 NUTRIENT BROTH

IDENTIFICATION : Staw colored liquid medium with label NB

TYPES :1. Meat infusionbroth

2. Meat extract broth
3.Digest broth

CONSTITUENTS : Lean meat/Meat extract

Peptone
NaCl
Water, Ph7.4

STERILISATION: Autoclaving at 121°c for 15 min.

USES : Basal liquid medium

Basis of most media.
BG-GLAS

PEPTONE WATER
IDENTIFICATION: Colourless transparent medium with
Lable PW.

CONSTITUENTS: Sodium
PeptoneChloride
Distilled water
TYPE OF
MEDIUM Basal liquid medium.

USES
1.Base for
2.Indole test.carbohydrate fermentation media
3.Demonstration of motility
 PEPTONE

IDENTIFICATION: Golden
Granulayellow
r powdercoloured.
SOURCE : Animal proteins (lean meat, heart muscle)
Vegetable proteins (soya flour)
by digestion with proteolytic enzymes

CONSTITUENTS : Peptone, Proteoses ,Amino acids

Inorganic salts , minerals( like K, Mg )
Accessory growth factors like Nicotinic acid,
Riboflavine )
USES Base for preparation of different media
Peptone water

leplon
 AGAR AGAR
Identification : Powder or dried shreds
Light straw coloure
Properties : Prepared from sea weed a long
chain
poly sacharide, melts at 95C& solidifies on
cooling to 42°C.
Use
Solifying agent to prepare solid media
Concentrations used -
Solid media - 2% Japanese agar
1.5% Newzealand agar
Semi solid agar - 0.2 -0.5%

Hard agar - 4-6% ( to prevent swarming growth of Proteus &

CI. tetani)
Agarose (purified agar ) 1% - for gel electrophoresis
 AGAR AGAR
ldentification : Powder or dried shreds
Light straw coloure
Properties :Prepared from sea weed a long chain
poly sacharide, melts at 95° C& solidifies on
cooling to 42 °C.
Use Solifying agent to prepare solidmedia
Concentrations used -
Solid media 2% Japanese agar
1.5% Newzealand agar
Semi solid agar - 0.2 -0.5%

Hard agar - 4-6% (to prevent swarming growth of Proteus &

Cl. tetani)
Agarose ( purified agar )1% -for gel electrophoresis
Both Agar Agar
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