ALKALINE PHOSPHATASE
KIT
(Mod. Kind & King's method)
Fare cetaminaon of ksi Phosphatase acy in
(Forinivw Diagnose Use On)
Summary
‘kane Phosphatase (AP) san onzyo ofthe Hyease
class of enzymes andatts man alae modu. ls ouné
a igh conariratens int tv, ile ret etal ane
atbores, Nomal lvls ar ape dependent anineease
uring bane davlopner. nseased les are assocalad
mai} wither and bore disease Moderate nceass are
Stonin Hodgkins dseasoandcongestuahea
Princie
ALP at an alkalina pH hyerlyses i Sodium
Phariphesohte oom eno. The Pherol format eats
wen Amioantpyine nthe posence of Poassum
Ferneyanie, 8 an exésng agent to fom ae colowes
compl. The irensiy of the caur fomed i decty
prepertnal othe ache ofALPpresentnte sap
*o pvi0e ata tyegeraphte
Lamocteyine KFA, cme
Norlraarene values
Teal AP Acsety 80-130KA Unis
I's reoarmandes tat each abort eth sown
nomalrangsepreseningts ae poplin
Contents ssTosts soTests
LY Bur Reagent Soni t2oml
Lz: Subst Reaper: Smite
La Cou Reoger emi 12m
S :Phonol Standard (10 gid) Sm) Sr
Storage staiity
Contents areal at 29°C th exp ented on
trelabot,
Reagent Preparation
Argan ara ea to use.
Sample material
Serum Fre fom henoysis ALPis reported o be sabe
a senor 3dayssi 2
LT
Procedure
Wlonghfiter—— $10nm(g46rmGron
Temperate arc
Urea ton
Assay
pat io fur oan ry ts utes baled as Bark),
Sanda), Conel (Test)
aon ss ce FT
Sows ym ee
Dita wr 108 19 18 10
pireReceis) | 10 10 10 10
ae
clad aw ara 7 Rrra nd a
‘oe 106
rea SararsS) 008
Twa adalowie sand alaTo fw Shas oda
Cowrapeniis) | 10 19 38 ae
sana 008
Mx wal ater each aditon. Nessie the absarbanoes of
‘he Blank (ft5.3), Siancar(Ab6 5S), Con (Abs. ), nd
Tests Tagan ced wate
Caleultons
Toa AP ecityinkA Uris =—————x 10
rorser)
Leary
Iraj acty exceeds 60 KAUns lt the samp
with ed wat and repost he assay Mul th ae
wih proper aluintactr
Nite
Incase cf mul sale tobe assayed simutanoousy,
nly ane lanka Sandard canbe runfrtne ene seis,
haweverfor each sanpleaContoand a Tes assay hasta
benunadsonaly
References
Ki, PRI, king EJ, (158) Clin Pat 7922
Vale. , (1875) Prac Cnc Blocharsty Ah Ed‘System Parameters
Reaction Endo Abs,
Wavelength: SiO
Zero Setting: Dist water
Ineub.Tamp. 370
Ineub. Time: 15min
DolsyTime : =
ReadTime : =
Nocofresd.
Inara =
‘Sample Vol. : 005ml
Reagent Vol. = 3.00
Standard = Cacubte
Frctor S
React. lope: Inceasing
Lnsaty DKA Unis
Unite kaUnte
srt
‘tana Dr. Arno Do Rego Bagh
Bamboim Gorpox 20. GOA. 403202, NDIA