Calkine SGPT (ALAT) Kit (Mod. IFCC Method) For the determination of SGPT (ALAN acy seu (ForinieoDiagrasic Use Ory) Summary SGPT sound in avait of esue ut ina found in tne vor, nessa love Tou i apa, cross, bstuctve jaundice and other hapatc diseases. Sight elevation ofthe enya also sen in myocadal Inarcton Principe SGPT (ALAT) calaaas the tanta of ano group betwen LAarine and a Kelogirate frm Pyruese ‘4 Gato. Te Pyruvate ome eas wth NADY n the resenceo Latte Dahyrogenase to frm NAD. The tate of oneaton of NADH Yo NAD ip mearued at @ {area absorbane wich proporional ot SGPT (ALA) actvtyinte samp ‘Sample matrl ‘Serum Frestannemolsis SGPT (ALATsropredtobe stablemserumtr3 deysal 28°, Procedure Waren ar orm Temperate arcisvciastc Ushi ton ‘Substrate StartAssay: Pow inioa candy etubelaboledss Test 7). ‘Addon a 7 Sequence asciare | a7 aya Reagentis) | 08m | 08m Sompiecr Contac) | _ozmi | atm aba i sy npoie or THA SarerResgr(c) [020 | Oa x wel and read e inal absarbanoe A & repost he absorbance reacng ater evr 1,28 mints. Cale ttomeanabsotancechangaper minus Ain) Normlreerence valu Serum (als) \atoaoutasre (female) nat Lar Its reamerended tat each labora estabish Hs own ral range representing spate popualon Contents 4x25m1 2x 150m Linge Reagort 4x 20m 2x 120 2: Sarr Reagor 20m 60m © Bovine Senn Coniol tm) tml Storage stability Conn ae sale t 24°C tne ery mentor on ne bes Reagent Proparation Reagonsarereadytoune, Working reagent:Forsargl start assaysa sing reagent Ss raqored Aworing agent my be mde a and when ested by mixing togete pars of Lt (Enzye Reagent and patsolL (Sata Reagor). Ts wokingreager is sable for at least 3. weots when sored at 28°C ‘MeratvelyO8mlofL’andO 2m ofL2 may leo be used ated of 1m ofthe working reagent det dg te assy ‘Control: Roconstute wr 1 of goed euaty OW. Leave forSminlohyeree endrixby sing to ave ohing The rocoto contol stables days won soos ‘hy soppradat2'C witout covariation. LT Sample Stats: soot Pete ta cnr tentbelnteds Tes) LeAtine + aogier + Lakme Ne ‘adiion 0 7 Pym + NADH sit = cate + mao? | Sequence sere | sre wertrgReagen [tom [Tom incall nas lonporaure Trt andael SenplerConea(6) [_02ri_[ Ott ecw andr eal abeorance alr inte & ‘epealibe absorbance reading ater every 1,289 ints Caluiate tie mean absortance ange per mute (anim Caeatons SubaateSample stat SOPT ALAT Acta Ut 286/30= AA/minx952 SGPT(ALAT)AcviyinUL 37 = AAI in x 1745 ‘TENPERATURE CONVERSION FACTORS Tsay] Desired Reporing Temperature Temperate | so wee are 25 rT) ae om 100138 are oss om 100 Linearity ‘The pred i near uo 500 URL at 37. the abserbanc charge (AA/ nin Joxcouds 0.250, usa ony thevalusothe fst wo inte ical theresut, orate the sampe 1 +Swthnoraleane NaCI0 9%) ard repeal te assay Resusx 1} Nite Samples nang very hgh aly show ery low ina stereance a otf the NADH consumed por the ta of macsramen, th e euspaiad an sa he samolesnsrepeatte assy. ‘Tha working reagent orth cine reagent shoud have ‘0 absoriance above ‘000 apis dist wate at 340 ri. Discard eager the absorbanceis blow 160 The cana i tacesbe to seoanday reference materi whch arrose toreerence prparatonscrmasteris, References IFCC. methods for the measurment of catalytic oncontators of exes, Jin. Cham. Cln Boctan, (1986 248 System Parameters Reaction UM. Kate Wavelength: 340m Zero Seting : Dstled Water Incub. Temp. 37°C Ineub. Time: DelayTime 60sec ReadTime 18sec Nocofread, : 4 Interval sec, Sample Vol 010i ReagentVol. 100m Standard - Factor 116 Reset Slope Decreasing Linearity = SOUL Unis ut tana Or Artenio De Rego Bagh, Bamboim Complex 2.0, GOA. 408 202, NDIA