E

Thank you for your interest in bioBUBBLE® products

and technology. bioBUBBLE has over 30 years
experience designing custom ultra-clean and
containment environments for a wide range of
applications. We have projects worldwide, from minor
renovations to complete turnkey solutions. We can
help you with your project from concept to completion.

We have the solution to transform

your space efficiently & creatively.

2
APPLICATIONS :
LABORATORY RENOVATION

LABORATORY ANIMAL HOUSING FOR BREEDING

& RESEARCH
PRIMARY CONTAINMENT FROM BSL/ABSL-2 TO
BSL/ABSL-4 LEVELS (P2, P3 & P4)
SCID PIG
PROCEDURAL AREAS
CELL SORTERS AND OTHER EQUIPMENT
QUARANTINE ENVIRONMENTS
FOOD SAFETY RESEARCH
REDERIVATION ROOMS
TOXICOLOGY RESEARCH
SPECIALIZED ANIMAL MODELS
HORTICULTURAL RESEARCH
VACCINE TESTING & PRODUCTION
AQUATIC RESEARCH
IMAGING SUITES
FACILITY DESIGN CONSULTING

CUSTOMIZED SOLUTIONS FOR YOUR APPLICATION

rev0321 www.bioBUBBLE.com | 1
 ENCLOSURES: ULTRA-CLEAN p22
Product Guide
AND CONTAINMENT ENCLOSURES FOR ROBOTS Containment
p4-5 enclosures for automated workflows provide personnel or
ARCHITECTURALLY RENOVATED VS product protection or both personnel and product protection
FREESTANDING ENCLOSURES with a flexible design.
Which design type is right for you?
p 23
p6-7 CLASS II EQUIVALENT ENCLOSURE provides
product and personnel protection
LABORATORY RENOVATION :
SAGE LABS | BREEDING FACILITY p24-25
p8-9 ANTEROOMS/AIRLOCKS bioBUBBLE Anterooms
are soft-walled enclosures that operate with dedicated positive
LABORATORY RENOVATION :
or dedicated negative airflows and are customized to suit the
CINVESTAV | BREEDING & RESEARCH FACILITY
needs of your specific application. Anterooms provide a space
p10-11 dedicated to donning and doffing PPE and also isolate individual
CLEAN ROOM, ISOLATION & BARRIER rooms within a facility.
ENCLOSURES bioBUBBLE Clean Rooms are soft-
walled, positive pressure enclosures with 50 – 100 air changes SUPPORT EQUIPMENT
per hour of HEPA filtered air supply. bioBUBBLE Clean Rooms p26-27
are completely customizable and constructed in any size or
configuration to suit the specific needs of your space and BEDDING DISPOSAL UNIT The bioBUBBLE
research. Bedding Disposal Unit is a lightweight, portable unit that draws
bedding dust, dander and allergens away from personnel
p12-13 and into the HEPA filter. This filtration greatly reduces
CUSTOM CLEAN SOLUTIONS : airborne particulates, minimizing the opportunity for disease
AUSTRALIAN BIORESOURCES | BREEDING & RESEARCH transmission throughout the vivarium.
FACILITY
IOWA STATE | CLEANROOMS FOR SCID PIG p28-29
AIR SHOWER The bioBUBBLE Air Shower supplies a
p14-15 high volume, low-velocity HEPA-filtered air stream to clean
NEGATIVE PRESSURE CONTAINMENT microparticles off personnel and materials upon entrance or
ENCLOSURES bioBUBBLE Containment Rooms exit of a controlled environment. This localized area of high
are soft-walled negative pressure enclosures with 50- 100 filtration serves as a barrier to prevent the transmission of
air changes per hour of HEPA-filtered exhaust. bioBUBBLE airborne contaminants.
Containment Enclosures are completely customizable and
constructed in any size or configuration to suit the specific p30-31
needs of your space and research. POWER UNIT WITH DIFFUSION HEAD
bioBUBBLE Diffusion Heads provide high levels of localized
p16-17 HEPA filtration. These units serve to drastically reduce
CUSTOM CONTAINMENT SOLUTIONS : the presence of dust, dander, allergens and other airborne
KANSAS STATE UNIVERSITY | FOOD SAFETY RESEARCH particulates in heavily populated rooms and high traffic cage
wash areas.
COLORADO STATE UNIVERSITY | BSL-3 TUBERCULOSIS
RESEARCH p32
WORKSTATION The bioBUBBLE Workstation is a
p18-19 portable, clean or contained environment. Negative Pressure
CUSTOM SOLUTIONS : Work Stations provide transportable biocontainment and
UNIVERSITY OF AUCKLAND | MIXED-USE VIVARIUM dedicated quarantine for materials or small populations.
UNIVERSITY OF CALGARY | REDERIVATION FACILITY Positive Pressure Workstations provide clean isolation for small
populations or materials. Use as a transport cart or a housing
p20-21 environment.
BENCHTOP BIOCONTAINMENT
ENCLOSURES bioBUBBLE Benchtop Biocontainment p33
Enclosures provide high levels of containment for all BSL-2 and RACK COVERS Custom manufactured to fit any size
BSL-3 applications. Enclosures are ideal primary containment rack, trolley, cart, or other vivarium fixture. Rack Covers are
solutions for cell sorters, incubators, centrifuges, microscopes, constructed of incredibly strong and durable vinyl coated
or any benchtop laboratory equipment. polyester fabric. bioBUBBLE creates the longest lasting covers
on the market.
2
[CLOCKWISE FROM TOP LEFT] bioBUBBLE (interior) at Kansas State University. Environmentally sustainable vivarium at Australian Bioresources. bioBUBBLE
Benchtop Enclosure for a cell sorter. bioBUBBLE Airlock and Bedding Disposal Unit at The University of Otago.

www.bioBUBBLE.com | 3
ARCHITECTURALLY RENOVATED
VS FREESTANDING ENCLOSURES
bioBUBBLE® Clean and Containment Rooms are custom,
soft-walled, enclosures constructed in any size or
configuration to suit the specific needs of your space
and research.
Two types of
designs available:
Freestanding and
Architecturally
Renovated Enclosures
Freestanding Enclosures
are flexible, portable, and
expandable. Simple design
and construction enables
equipment to be used as
a permanent solution that
can also be easily stored or
modified as needs change. RED = CONTAMINATED AIR

BLUE = HEPA-FILTERED AIR

YELLOW = AMBIENT AIR

< FREESTANDING NEGATIVE

PRESSURE CONTAINMENT
ENCLOSURE

4
 BLUE = HEPA-FILTERED AIR SUPPLY

YELLOW = HVAC SUPPLY :

CONDITIONED AND QUESTIONABLE AIR REDIRECTED OUTSIDE
bioBUBBLE CUSTOM THE ENCLOSURE FOR FILTRATION BY bB POWER UNITS
SOFT DUCT SYSTEM

ARCHITECTURALLY
RENOVATED
POSITIVE PRESSURE
CLEAN ENVIRONMENT >

VINYL WALL

bioBUBBLE
POWER UNIT

Architecturally Renovated
Enclosures provide a unique
solution for spaces with
architectural challenges. This
construction type uses a
combination of existing walls
with bioBUBBLE soft walls,
custom dividers and ductwork
to provide HEPA filtration to any
space. Architecturally renovated
enclosures provide access to
existing cabinets, fixtures and
workstations while maintaining a
clean or contained environment
and maximizing floor space.

WHICH DESIGN TYPE IS RIGHT FOR YOU?

DO YOU NEED ACCESS TO FIXTURES, SHELVES, & CABINETS?
DO YOU UTILIZE BUILT-IN WORKSTATIONS?
CONTACT A bioBUBBLE CONSULTANT TO HELP.

bioBUBBLE® Freestanding and Architecturally Renovated Enclosures provide

an economical, long-term solution that will help you quickly transform your
valuable space into a world-class research environment.
www.bioBUBBLE.com | 5
LABORATORY RENOVATION

bioBUBBLE sales team

associates can help you:
 ave Time & Cost
S
Simplify Design
Increase Flexibility
Achieve a Higher
Level of Cleanliness or
Containment

Clean Room Expansion Phase 4 116" 116" 116"

2016 29"

36"

SAGE LABS 118"

CHALLENGE | Expand breeding and #11

206"
research operations for genetically
Positive
290" Pressure Future
Freestanding
Expansion
modified animals.
#10
116"x290"x100"H 87 1/4" Positive Pressure
74 ACPH Phase925ACPH
92 ACPH Freestanding
Red Tinted 134"
116"x290"x84"H 116"x290"x84"H
Walls 134"x206"x100"H
73 ACPH

SOLUTION | Outfit a reclaimed Red Tinted Walls

warehouse space with Ultra-Clean

46" 314"
107"
#9
Enclosures to create separate
130" 66" 5" 68"
39 1/2" Positive Pressure
Freestanding 128"
housing environments. This design #12 #1 #2 #3 128"x230"x100"H
79 ACPH

also incorporates a bioBUBBLE Positive Pressure

Freestanding
Positive Pressure
Freestanding
Positive Pressure
Freestanding
146"x272"x100"H
Positive Pressure
Freestanding
146"x272"x100"H

Containment Enclosure for quarantine, 272"

130"x272"x100"H
74 ACPH
146"x272"x84"H
93 ACPH
77 ACPH
Red Tinted Walls
77 ACPH
Red Tinted Walls
116 3/4"

BED/FEED
230"

Air Showers, and bioBUBBLE soft STORAGE

172

ducts.
NU-677-600

RESULTS | A savings of more than

VESTIBULE
32.75" x 77.6"
173
#4 #5

64% compared to hard construction 195 3/4"

Positive Pressure
Freestanding
Positive Pressure
Freestanding
146"x146"x100"H
CLEAN

and facility outfit costs.

72 ACPH ROOM
146"x146"x84"H 72 ACPH
171
86 ACPH Red Tinted Walls

88" 40"
BARRIER
OFFICE
168
#7 91 ACPH #8 #6
154"x132"x84"H Positive Pressure Positive Pressure
Positive Pressure
121" Freestanding Freestanding Freestanding
120"x124"x84"H 154"x132"x100"H 154"x132"x84"H
132"
83 ACPH 77 ACPH 91 ACPH
Red Tinted Walls Red Tinted Walls

124" 154" 154" 154"

54" 54"
701"

6
 Breeding Facility
We had explored expanding
this space with hard walled
animal holding rooms and
the cost was estimated at
$5.6 Million…. We turned
our focus toward utilizing
bioBUBBLES to both lower
cost and provide us the
flexibility that we needed
as a growing business. The
price tag for our expansion
utilizing bioBUBBLES,
upgrading our auto watering
system, adding a tunnel
washer, expanding our
lab, adding 5 offices and a
conference room was just
under $2 Million.
—DEB KNOERZER, SAGE LABS

www.bioBUBBLE.com | 7
Research Facility CINVESTAV
PROJECT SUMMARY | CINVESTAV is a uniquely designed three-story vivarium with the capacity
to house over 25,000 SPF/ MPF rodents and an estimated annual breeding production of 35,000
to 50,000 animals of 65 different biomodels.

FEATURES INCLUDE | LEGEND

bioBUBBLE positive pressure, HEPA filtered

Lockable, Swinging
Dirty Door Location
Lift
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Location

environments for housing. Dirty

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to prevent the transmission of airborne
contaminants.
Custom soft ductwork distributes HVAC air
Company: Drawing Is Accepted By: PO#:
CINVESTAV
LEGEND
Room/Title:
Level 3_Segundo Nivel Date: Date:
29 May 2014

supply throughout each level.

This Drawing Must Be Printed To 17" x 11" Paper Lockable, Swinging
Type: Door Location
Scale:
PHASE 2 Dirty Not to Scale
Printing Layout Scale Must Be 100%
Signature: Strip Door
Lift

S
OD: For Architectural Scale To Be Used Drawn By: Location
Jack

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Material UP-3 Power Unit

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Company: Drawing Is Accepted By: PO#: LEGEND

CINVESTAV
Room/Title: Lockable, Swinging
Level 2_Primer Nivel Date:
Dirty
Date: Door Location
29 May 2014
Lift This Drawing Must Be Printed To 17" x 11" Paper
Type: Strip Door
PHASE 2 Signature:
Scale:
Location
Not to Scale
Dirty Printing Layout Scale Must Be 100%
OD: Material Drawn By:
For Architectural Scale To Be Used bioBUBBLE
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Company: Drawing Is Accepted By: PO#:

CINVESTAV
 2016
TURNKEY
FACILITY OF
THE YEAR

CINVESTAV / MEXICO CITY, MX

www.bioBUBBLE.com | 9
CLEAN ROOM, ISOLATION &
BARRIER ENCLOSURES
bioBUBBLE Clean Rooms are soft-walled,
positive pressure enclosures with
50 – 100 air changes per hour of HEPA
filtered air. bioBUBBLE Clean Rooms are
completely customizable and constructed
in any size or configuration to suit
the specific needs of your space and
research. Available in two basic design
styles: Freestanding Clean Rooms and
Architecturally Renovated Clean Rooms.

bioBUBBLE Clean Rooms are

constructed using:
•a
 modular anodized aluminum
framework
•v
 inyl skin with Velcro®-type connections
•b
 lowers with long lasting, energy
efficient, electrically commutated
motors
•9
 9.99% efficient HEPA filters

bioBUBBLE Clean Rooms are

economical and durable enough to
withstand the most demanding research
and manufacturing environments.

Some of the very first bioBUBBLE enclosures

have been in continuous operation,
24 hours a day, 7 days a week, for over 20 years.

10
APPLICATIONS CLEAN ROOM, ISOLATION & BARRIER ENCLOSURES
Animal Housing - Immunocompromised, SPF, Gnotobiotic, Conventional
Procedural Space • Aquatic Research • Class 100 / ISO 5 Manufacturing
Processes • Product Mixing & Preparation • Toxicology •
Pharmaceutical Processing • Preparation & Packaging Horticultural Research
• Food Processing, Preparation & Packaging
Customized Solutions – Call us to discuss a solution for your application.
FEATURES & BENEFITS
Ultra-Clean Environment [up to class 100/ISO 5]: bioBUBBLE
Clean Rooms provide an ultra-clean environment to control
dust, allergens, dander and airborne contaminants. Conditions
are created with high levels of HEPA filtration and mass airflow.
bioBUBBLE Clean Rooms provide the ideal conditions for housing
any species, animal model, or performing any procedural work
requiring a controlled environment.
User Friendly/Comfortable: Clear vinyl walls facilitate
supervision by allowing complete visual access without the need
to physically enter the clean room. The clear vinyl also reduces
the feelings of isolation and claustrophobia which can occur in
standard hard walled laboratories. Communication is improved via
visual access, creating a more user-friendly environment.
 nvironmental Separation: bioBUBBLE Clean Rooms provide
E bioBUBBLE Clean Rooms easy to clean and maintain. Enclosures
environmental separation between individual groups, species or
studies within the same room or shared space.
Cost Savings: bioBUBBLE Clean Rooms reduce costs by
45–50% when used as an alternative to hard walled construction
in both new and renovated facilities. In addition, this investment is
made in transferable capital equipment rather than a permanent
structure.
Flexible Construction: bioBUBBLE systems can also drastically
simplify building ventilation (HVAC) systems, increasing cost
<
INTEGRATED ULTRA-
CLEAN HOUSING AND
PROCEDURAL SPACE WITH
TWO BIOSAFETY CABINETS
DOCKED TO EXTERIOR OF
bioBUBBLE CLEAN ROOM
savings and reducing construction timelines. bioBUBBLE Clean
Rooms are mounted on high-quality, 5” hospital-grade casters
making them extremely portable and easy to move. Modular
designs are also available, providing the benefit of docking multiple
rooms together to increase size as needed.
Installation and Training: bioBUBBLE offers complete on-site
installation and training performed by qualified bioBUBBLE
technicians, with little to no interruption in the workflow of the
facility.
HEPA Filtration: 99.99% on 0.3 micron particles- military std MIL-
STD-282/ industry std IEST-RP-CC-034
Easy to Clean: Zippered and Velcro® type access points make
tolerate all standard sterilants and disinfectants.
Energy Efficient: bioBUBBLE Clean Rooms are extremely energy
efficient. Power Units use electronically commutated motors for
energy savings.
Options: Anterooms, multiple door styles, access control system
& keypads, pass through boxes, custom docking systems, frame
mounted shelving and tables, removable perimeter rodent
barriers, ports for ventilated caging and auto watering systems,
and heat resistant, opaque, or tinted vinyl.
www.bioBUBBLE.com | 11
Breeding Facility
AUSTRALIAN BIORESOURCES
CHALLENGE | To create a low-cost, energy-efficient breeding and research facility.
SOLUTION | Construct a simple building on a rural site to lower the cost of the initial investment. Outfit the
building with bioBUBBLE Systems to provide positive pressure enclosures each with approximately 80 air
changes per hour of HEPA-filtered air through high efficiency, low noise filtration units. Housing capacity
is expandable to a population of 42,000. Vivarium model significantly reduces energy consumption and
operating costs and exceeds the efficiency requirements of the Building Code of Australia.

bBCR = bioBUBBLE Clean Room

WB
WORK BENCH
bBCR bBCR bBCR bBCR bBCR bBCR
1 2 3 4 5 6
CS
CHANGING
STATION

WB CS WB CS WB CS WB CS WB CS WB CS

INTERNAL
bioBUBBLE DIVIDER

MIXED USE HOUSING / PROCEDURAL

CS WB CS WB CS WB
POWER UNIT CS WB CS WB

bBCR bBCR bBCR bBCR

7 8 9 10

bBCR
11

14,000 cage
capacity/multiple
enclosures
subdivide a large
open space

12
 SCID Pig Laboratory

SCID Pigs
Cleanrooms for
Personnel Flow
LEGEN
IOWA STATE UNIVERSITY
9 Power Units
CHALLENGE | How to raise
deliver approximately 47 - 53SCID
ACPH pigs in a clean environment. 1818 Powe
of HEPA filtered air
throughout Clean Room System
SOLUTION | Create a clean 10'-7"
Clean
environment with high levels of Storage Transfer Cart
3'-1 1/4"
HEPA filtration
Power in annear
Units oldfloor
storage
drains
Clean Potentia
area. Multi stage anteroom
are on 12" elevated bases 9'-0" Personnel
Contamin
CR #2 Entrance CR #3
for personnel and materials
for spray downs
+
entering the clean barrier. Unique
++ ++
Bench

architectural renovation design Farrowing Pen

7' x 7' pen
Farrowing Pen
7' x 7' pen 48" Strip
Clean Gown
allows for multiple large animal Storage Area
18'-6" 6'-0"
+ 18'-6"

Storage
environments, access to auto

floor drains
Environme
watering systems and maximizes Separation D
31'-9 1/4"
the usable floor space. HEPA Farrowing Pen
7' x 7' pen Street Clothes/
Farrowing Pen
7' x 7' pen
filter unit intakes located near 7'-0"
Degown Area
+
the floor capture the highest

Bench
Clear Vinyl
quantity of airborne particulates.
CR #1 CR #4
Internal dividers give flexibility 6'-0"

and allow you to adjust the size of ++ ++

10'-2"
Animal Pen Animal Pen
10'-2" Power Unit A
the enclosure as needs change. 6' x 7' pen 6' x 7' pen

Drains are located outside the HEPA Duct

hose

clean environment. sink/hose

area
Opaque W

RESULTS | Minimal renovation

12'-0" 11'-6"
costs to convert a storage room
Alterna
into an ultraclean environment Power U
for SCID pigs. 34'-4 1/8" Locatio

www.bioBUBBLE.com | 13
NEGATIVE PRESSURE
CONTAINMENT ENCLOSURES
bioBUBBLE Containment Enclosures are soft-
walled, negative pressure enclosures with
50 – 100 air changes per hour of HEPA-filtered
exhaust. bioBUBBLE Containment Enclosures
are completely customizable, and constructed
in any size or configuration to suit the specific
needs of your space and research. Available
in two basic design styles: Freestanding
Containment Rooms and Architecturally
Renovated Containment Rooms.

bioBUBBLE Containment Rooms are constructed

using:
• a modular anodized aluminum framework
•v inyl skin with Velcro®-type connections
•b lowers with long lasting, energy efficient, Architecturally Renovated Containment Room
electrically commutated motors
• 99.99% efficient HEPA filters

bioBUBBLE Containment Enclosures are

economical and durable enough to withstand the
most demanding research and manufacturing
environments. Some of the very first bioBUBBLE
enclosures have been in continuous operation, 24
hours a day, 7 days a week, for over 20 years.

Hybrid Freestanding Enclosure

50 100
Air changes per hour of HEPA-FILTERED exhaust
under negative pressure

14
APPLICATIONS NEGATIVE PRESSURE CONTAINMENT ENCLOSURES
BSL-2, BSL-3 & BSL-4 applications • ABSL-2, ABSL-3 and ABSL-4 levels
(P2, P3, and P4) • BSL-3-Ag • Quarantine environments • Surgical suites

or procedural areas • Isolation of laboratory equipment • Biocontainment of

pollens and plant matter for horticultural research • Food safety research

FEATURES & BENEFITS

Superior Containment: High levels of HEPA-filtered exhaust and
mass air displacement provide the best containment of airborne
pathogens, contaminated dust, allergens, and other airborne
contaminants. The re-circulated HEPA exhaust also provides
an added barrier of cleanliness surrounding the containment
environment.

User Friendly/Comfortable: Clear vinyl walls facilitate

supervision by allowing complete visual access without the need
to enter the containment environment. The clear vinyl walls also
reduce feelings of isolation and claustrophobia, which can occur
in standard hard walled containment rooms. Communication is
improved via visual access, creating user-friendly environment.

Environmental Separation/Isolation: bioBUBBLE Containment

Rooms provide environmental separation and isolation of groups,
species or studies within the same room or shared space.

Cost Savings: bioBUBBLE Containment Rooms, when used

as an alternative to hard walled construction in both new
and renovated facilities, reduce capital costs by 30% - 45%.
This investment is also completely transportable and can be HEPA Filtration: 99.99% on 0.3 micron particles- military std MIL-
relocated or retrofitted as location or applications change. STD-282/ industry std IEST-RP-CC-034

Flexible Construction: bioBUBBLE Containment Rooms are
mounted on high quality, hospital grade casters, making them
extremely portable and easy to move. Modular designs are also
available, providing the benefit of docking multiple rooms together
to increase size as needed.
Installation and Training: bioBUBBLE offers complete on site
installation and training performed by qualified bioBUBBLE
technicians, with little to no interruption of the workflow in your
facility.
Easy to Clean: Zippered and Velcro® -type access points make
bioBUBBLE Containment Enclosures easy to clean and maintain.
Enclosures tolerate all standard sterilants and disinfectants.
Energy Efficient: bioBUBBLE Containment Enclosures are
extremely energy efficient. Power Units use electronically
commutated motors for energy savings.
Options: Anterooms, multiple door styles, access control system
& keypads, pass through boxes, custom docking systems, frame
mounted shelving and tables, removable perimeter rodent
barriers, ports for ventilated caging, auto watering systems, and
heat resistant, opaque, or tinted vinyl.

www.bioBUBBLE.com | 15
Primary Containment -
Food Safety Research KANSAS STATE
UNIVERSITY
Containment
Enclosure
CHALLENGE | To create a primary
containment environment with the
ability to handle large equipment and to
provide liquid containment.

SOLUTION | Design and install a

customized soft-wall enclosure reaching
a height of 17’ (5.2m) to accommodate
oversized equipment. The enclosure
also incorporates sloped ceilings for
water runoff from equipment washdown
procedures and has sealed vinyl
connections and a spill berm at the floor
for effluent containment.

RED = CONTAMINATED AIR

BLUE = HEPA-FILTERED AIR

YELLOW = AMBIENT AIR

16
 Tuberculosis Research
Primary Containment -
COLORADO STATE UNIVERSITY ABSL-3
Tuberculosis Research Laboratory
CHALLENGE | To economically renovate and create a primary containment environment within an
existing space that will exceed ABSL-3 research guidelines.

SOLUTION | Create an environment that maximizes floor space by designing an architecturally

renovated bioBUBBLE enclosure into the existing room. The custom, soft-walled, negative pressure
environment includes 100% HEPA-filtered exhaust and integrates an anteroom into the design for
donning and doffing procedures while creating a buffer zone between containment space and hallway.

HEPA-FILTERED AIR
HEPA-FILTERED AIR EXHAUSTED TO PLENUM BETWEEN VINYL & HARD CEILING SUPPLIED TO
ANTECHAMBER
VINYL CEILING

BIOSAFETY CABINET

bioBUBBLE
POWER UNIT

www.bioBUBBLE.com | 17
 UNIVERSITY OF AUCKLAND
Mixed-Use Vivarium
Medical School Vivarium
CHALLENGE | Create research and breeding vivarium from space deemed unsuitable.
Accommodate multiple species, applications, and health statuses.

SOLUTION | Construct positive and negative bioBUBBLE enclosures to provide environmental

separation between housing groups, procedural, and surgical spaces.
RATS

RESULTS | Ultra-clean housing for multiple species in the same hard room.
14

Designated biocontainment area within the main vivarium. RATS

16

RATS
PC2 PC2 PC2 15
1 2 3 WAREHOUSE 4

35%
MICE
17
HOUSING HOUSING
19 20

WAREHOUSE 2

savings HOUSING
21
GUINEA
PIGS
Guinea
Pigs

WAREHOUSE 3

as compared HOUSING
HOUSING
23

to hard-walled
22

RABBITS

renovation
Rabbits

FACILITY
ENTRANCE/
GOWNING
PC2 LAB/
ANTEROOM

costs.
PERFUSION PC2
Exit
SIDE A SIDE B
SMALL LARGE
ANIMAL ANIMAL

Hallway
OUTSIDE
ANIMAL AREA

ELEVATOR
ROOM
DIRTY CLEAN BEDDING
FOOD
CAGE WASH CAGE WASH STORAGE
STORAGE

RODENTS
3

BOTTLE
PREP OP PREP OP PREP OP PREP OP
WASH
1A 1B 1C 2

OP OP OP OP

RODENTS
6A STORAGE

BEHAVIORAL LAB
7
AREA RODENTS
6B

WAREHOUSE 1
AVIAN
ANTEROOM
LAB
8 RODENTS
5

AVIAN
AREA METABOLIC
10
Hood Hood

18
UNIVERSITY OF CALGARY

Rederivation Facility
Rederivation Facility
CHALLENGE | Subdivide a 2,000 sq ft hard room to create suitable housing and procedural areas
for an in-house rederivation operation.

SOLUTION | Design two large bioBUBBLE Environments, one containment, one ultra-clean, for
infected vs. clean animal groups. Both enclosures feature antechambers for personnel and materials
traffic to support each individual environment. Provide internal separation for embryo transplant
procedures within the ultra-clean enclosure. Equip each enclosure with Changing Stations and
Bedding Disposal Units for self-sustained cage changing procedures. Create air barriers between the
exterior and interior of the hardroom as well as between the barrier and containment zones inside
the hardroom using Anterooms with bioBUBBLE Air Showers.

RESULTS | The first

bioBUBBLE rederivation
facility design to
incorporate both negative
and positive pressure
bioBUBBLE enclosures
within the same hard
architectural space.
Eliminates outsourcing,
simplifies the process and
saves time and money.

www.bioBUBBLE.com | 19
BENCHTOP BIOCONTAINMENT
ENCLOSURES
The bioBUBBLE Benchtop Biocontainment Enclosure
(BBE) provides high levels of primary containment for
BSL-2 and 3 applications. BBE’s are ideal primary
containment solutions for cell sorters, incubators,
centrifuges, microscopes, or any benchtop laboratory
equipment.

The BBE captures aerosols and other airborne

particulates generated during laboratory procedures
and surpasses Class 1 specifications:

•A
 irflow 75+ fpm through the user access.

•6
 -8 air changes per minute with little turbulence.

•A
 ir is drawn into the BBE on all sides, decreasing
uncirculated air pockets.

EASY TO USE
EXCEEDS CLASS 1
STANDARDS while minimizing
noise & vibration
20
BENCHTOP BIOCONTAINMENT ENCLOSURES

FEATURES
Quiet: Flexible material minimizes noise and vibration.

Energy Efficient: Fans are 75% efficient. Low back pressure HEPA FILTER
allows fans to run at lower speeds to decrease energy use. POWER UNIT
Clear construction transmits ambient light, eliminating the
need for an internal light source to maintain a low heat load and
reduce electrical consumption.

Durable: Materials withstand the effects of harsh laboratory

sterilants and disinfectants.

Easy Access: Innovative design facilitates access through sides

of enclosure for maintenance and calibration. Custom access
ports for use and service.

Remote Display: Monitors HEPA life, airflow and temperature.

Small Footprint: Custom sized to fit equipment.

Customizable: Options include size and style, access panel

location, remote display, magnetic or hook and loop closures.
Decon bags available for use with hydrogen peroxide vapor,
chlorine dioxide, etc.

TECHNICAL
SPECIFICATIONS
Power: 110-240 VAC; 1.75 A

Airflow: 75+ fpm at user access

Heat load: 214 BTU/hr

Weight: Approx. 138 lbs

HEPA filtration: 99.99% or better on 0.3 micron particles,

Military Std MIL-STD-282 / Industry Std IEST-RP-CC-034

HEPA replacement: Annual certification recommended RED = CONTAMINATED AIR

BLUE = HEPA-FILTERED AIR

YELLOW = AMBIENT AIR

www.bioBUBBLE.com | 21
ENCLOSURES FOR ROBOTS
bioBUBBLE containment enclosures for automated workflows provide personnel or product protection or
both personnel and product protection with a flexible design. bioBUBBLE HEPA filtered containment and clean
enclosures are custom designed in any size or shape for liquid handling robots, plate stackers, plate sealers,
automatic pipettors or any other automated workflow.

FEATURES
Clear vinyl allows visual access to all
sides of equipment

Soft walls reduce vibration

Easily moved

Specialized design to customize access,

size and shape

FLEXIBLE CONTAINMENT
SOLUTIONS for laboratory
automation.
22
Class II equivalent enclosure
provides product and personnel protection

3D View: HEPA In & HEPA Out Sony Cell Sorter Enclosure

PU2525 HEPA Air

Handler Provides Clean bioBUBBLE Power Unit
Positive Pressure to Sony Exhausts Clean HEPA
for Product Protection Out Top

Large Zipper Access

for Access to Entire Front
of Positive Enclosure

Zipper Patches for

Left Side Access to Sony

PU1818 HEPA Air

Handler Provides Negative
Pressure for Large Outer
Enclosure
Magnetic Openings
Along Front of Enclosure
(Open Downward)

88"
Top View: Airflow Pattern for HEPA In & HEPA Out Enclosure

62"

44"

44"
22"
4"

62"

www.bioBUBBLE.com | 23
ANTEROOMS/AIRLOCKS
bioBUBBLE Anterooms are soft-walled
enclosures available as a Freestanding Units or
Architectural Renovations. Anterooms operate
with dedicated positive or dedicated negative
airflows. Enclosures can be designed to convert
between positive and negative pressures
to adapt to changing environmental needs.
bioBUBBLE constructs Anterooms in any size
or configuration, customized to suit the needs
of your specific application. The bioBUBBLE
Anteroom/ Airlock is optional on all bioBUBBLE
Clean Rooms and Containment Enclosures.

bioBUBBLE constructs Anterooms using:

•a  modular anodized aluminum framework
•v  inyl skin with Velcro ® -type connections
• long lasting, energy efficient blowers
•9  9.99% efficient HEPA filters

bioBUBBLE Anterooms are economical

and durable enough to withstand the most
demanding research and manufacturing
environments.

80 100
HEPA-FILTERED air changes per hour
negative, positive or convertible pressure

24
APPLICATIONS ANTEROOMS/AIRLOCKS
Dedicated Doffing Space: Negative Pressure bioBUBBLE
Anterooms provide secondary containment barrier areas for
personnel to perform doffing procedures prior to exiting BSL-2,
BSL-3 and BSL-4 areas.
Dedicated Donning Space: Positive Pressure bioBUBBLE
Anterooms provide a HEPA-filtered, exclusion environment
that controls entry into ultra-clean and isolation environments.
Positive Pressure Anterooms also maintain the integrity of sterile
gowning materials and personal protective equipment (PPE).
Docking Systems: bioBUBBLE Anterooms provide a suitable
location for the introduction of sterile materials under aseptic
conditions. bioBUBBLE designs and manufactures custom
docking systems to mate with autoclave containers, glove box
isolator ports, pass through boxes, or any other equipment.
FEATURES & BENEFITS
Environmental Separation: The bioBUBBLE Anteroom
offers a portable, low cost, non-invasive method of achieving
environmental separation within the facility, regardless of the air
balance of the hard room.
Isolation: bioBUBBLE Anterooms effectively isolate individual
hard rooms, allowing you to utilize mixed-use corridors, while
eliminating concerns of cross-contamination between individual
rooms.
Assist Standard Operating Procedures: bioBUBBLE
Anterooms provide dedicated areas for implementation of
standard operating procedures (eg. donning and doffing) and aid
in protocol reinforcement.
Air Barrier: bioBUBBLE Anterooms create air barriers for
effective isolation of specific rooms and areas within the facility.
Solve Air Balance Problems: bioBUBBLE Anterooms
compensate for improper air balancing due to older or
problematic building ventilation systems.
Eliminate Disease Transmission: bioBUBBLE Anterooms
reduce the possibility for airborne transmission and transference
of pathogen laden airborne particulates.
Regulatory Compliance: bioBUBBLE Anterooms assist with
personnel compliance of regulatory guidelines without the need
for permanent, invasive and expensive hard construction.
<
LOW PROFILE POSITIVE PRESSURE
ANTEROOM USED AS AIR BARRIER IN
HIGH TRAFFIC CORRIDOR
Installation and Training: bioBUBBLE offers complete on-site
installation and training performed by qualified bioBUBBLE
technicians, with little to no interruption of the workflow in your
facility.
Energy Efficient: bioBUBBLE Anterooms are extremely energy
efficient. The Power Units use electronically commutated motors
for energy savings.
www.bioBUBBLE.com | 25
BEDDING DISPOSAL UNITS
bioBUBBLE Bedding Disposal
Units are lightweight, portable
units designed with the
respiratory health of animal
care workers in mind. Each unit
draws bedding dust, dander and
allergens away from personnel
and into the HEPA filter. This
filtration greatly reduces airborne
particulates, and minimizes
the opportunity for disease
transmission throughout the
vivarium. Use of a bioBUBBLE
Bedding Disposal Unit creates
a pleasant and allergen-free
working environment.

• Easy to use, portable &

affordable.

• Highly effective – Capture

velocity of 100 feet per minute
at outside rim of standard 30
gallon receptacle.
Bedding Disposal Unit
• Custom height modification
available to accommodate any
size receptacle.

• Soft, flexible surfaces minimize High levels of filtration

cage breakage – no hard
surfaces come into contact in the cage wash area greatly
with cages.
reduces airborne particulates,
• Open design provides a more
ergonomic, friendly user
& minimizes the opportunity for
experience. No reaching or disease transmission throughout
leaning to empty cages.
the vivarium.

26
 BEDDING DISPOSAL UNITS

FEATURES
Lightweight: Aluminum frames and
lightweight synthetics are used to create
the lightest units on the market.

Portable: Heavy-duty 5” casters with

brakes.

Capacity: Accepts all standard biohazard

boxes and waste receptacles. HEPA-FILTERED
EXHAUST
Odor control: Decreases the volume
of damp airborne particulates and
significantly reduces odors.

Options: Custom height modification to

accommodate taller receptacles. Custom
modifications to accept wider receptacles.

TECHNICAL
SPECIFICATIONS
Power requirements:
115 Volts, 6.6 Amps [8.0A at start-up]
Other voltages available

Airflow:
200+ FPM at filter face

Weight: DUST,
170 lbs [77 kg] DANDER,
ALLERGENS
Dimensions: AND
30” x 36” x 55”H AIRBORNE
[76.2 x 91.4 x 139.7 cm] Standard CONTAMINANTS

HEPA Filtration:
99.99% on 0.3 micron particles-
military std MIL-STD-282/ industry std
IEST-RP-CC001.5

HEPA Filter:
24” x 24” x 12”
Replacement recommended every
3-5 years; annual certification
recommended

Prefilters:
24” x 24”
Change regularly or when visibly dirty

www.bioBUBBLE.com | 27
AIR SHOWERS
The bioBUBBLE Air Shower provides a high
volume, HEPA-filtered air stream to create a
localized area of filtration. This serves as a
barrier to prevent the transmission of airborne
contaminants while providing an ultra-clean
environment for gowning into or out of a controlled
environment. Air Showers can also compensate for
improper air balance in building ventilation systems
by providing high levels of filtration at doorways
and entry points. Additionally, the Air Shower
provides a psychological reinforcement to improve
compliance with standard operating procedures.

• Custom designs fit any space and suit any

application.
• Limit disease transmission in hallways and
corridors.

• Reduce dust, dander and allergens.

• Compensate for improper air balance in building

ventilation systems.

• Provide a localized filtered environment for

sensitive procedural work.

The bioBUBBLE
Air Shower
provides a low
velocity, high volume,
HEPA-filtered air stream
to create a localized area
of high filtration.

28
 AIR SHOWERS

FEATURES
Custom Size: Soft duct of any size. Quiet: Low noise and vibration levels.

Portable: Heavy-duty 3” casters with brakes.
Odor Control: Mass air flow through HEPA filter decreases the
number of damp airborne particulates and significantly reduces
odors.
Energy Efficient: bioBUBBLE Air Showers are extremely energy
efficient. The Power Units use electronically commutated motors
for energy savings.
Power Unit Footprint: 18” x 18”, overall height based
on duct design.

HEPA
SUPPLY AIR

bioBUBBLE
POWER UNIT

BLUE = HEPA-FILTERED AIR

YELLOW = QUESTIONABLE AIR

TECHNICAL SPECIFICATIONS
Power Requirements: 110-240 VAC; 4.35 A

Airflow: up to 650 CFM

Noise Level: +
_ 65 dBA

Weight: 144 lbs [65.3 kg]

HEPA Filtration: 99.99% on 0.3 micron particles- military std MIL-STD-282/ industry std IEST-RP-CC-034

HEPA Replacement: AFH-1636; Indicator light flashes when HEPA replacement is required. Certify annually.

Prefilters: Change regularly or when visibly dirty

www.bioBUBBLE.com | 29
POWER UNIT WITH
DIFFUSION HEAD
bioBUBBLE Diffusion Heads are heavy duty portable
units designed to filter ambient air and increase room
air changes, drastically reducing the presence of dust,
dander, allergens and other airborne particulates. This
compact, energy-efficient unit is equipped with a HEPA
filter certified to capture 99.99% of particulates at 0.3
microns. Corrosion resistant cabinet withstands most
commercially available sterilants and disinfectants.
LCD touch display controls air speed and monitors
HEPA life. Two stage air filtration with replaceable
primary filters extend the life of the HEPA filter.

• Lightweight portable units.

• 
Small footprint allows for optimal placement in any
space.

• Does not disrupt room air balance.

• Custom ducts available to maximize circulation.

More air changes

+ 99.99%
HEPA filtration
= superior air quality.
30
POWER UNIT WITH DIFFUSION HEAD

FEATURES
Portable: Lightweight design; Heavy-duty 3” braking casters for greater
mobility.

Odor Control: Mass air flow through HEPA filter decreases the number
of damp airborne particulates and significantly reduces odors.

Quiet: Low noise and vibration levels.

Energy Efficient: bioBUBBLE Diffusion Heads are extremely energy

efficient. The Power Units use electronically commutated motors for
energy savings.

TECHNICAL SPECIFICATIONS
Power Requirements: 110-240 VAC; 4.35 A

Airflow: up to 650 CFM

Noise Level: + 65 dBA

Weight: 144 lbs [65.3 kg]

HEPA Filtration: 99.99% on 0.3 micron particles- industry std IEST-RP-

CC-034

HEPA Replacement: AFH-1636; Indicator light flashes when HEPA

replacement is required. Certify annually.

Prefilters: Change regularly or when visibly dirty

AIRFLOW DIAGRAM
TOP VIEW
360º DUCT STYLE

www.bioBUBBLE.com | 31
WORKSTATION
The bioBUBBLE Workstation is a portable, clean or contained environment. Negative Pressure Workstations
provide transportable biocontainment and dedicated quarantine for materials or small populations. Positive
Pressure Workstations provide clean isolation for small populations or materials. Use as a transport cart or a
housing environment.

Workstations provide:
• Provide environmental separation between small
groups or studies within the same space.

• Temporary or permanent housing within

procedural rooms, surgical rooms and imaging
areas.

• Can design to dock to directly to equipment for

isolated transfer of materials and animals.

Options for shelves, battery backup to

provide power during transport, clear or
opaque vinyl

TECHNICAL
SPECIFICATIONS
Power Requirements: 110-240 VAC, 1.75 Amps

Power: 84 Watts

Heat Load: 214 BTU/hr

Size: 56”W x 30”D x 80”H

Weight: 235 lb;278 lbs with the battery option

Airflow: Max 150 @115 Volts

HEPA filtration: 99.99% or better on 0.3 micron particles-

IEST-RP-CC-034, certify annually

32
RACK COVERS
bioBUBBLE Rack Covers are custom manufactured
to fit any size rack, trolley, cart, or other vivarium
fixture. Rack Covers are constructed of incredibly
FEATURES & BENEFITS
durable vinyl coated polyester fabric and Velcro® - • Custom manufactured to fit your racks, carts or
type connections creating the longest lasting covers trolleys.
on the market. Use Rack Covers for the transport
• Heavy-duty fabrics create the most durable covers
of both clean and dirty materials to maintain the
on the market.
highest possible cleanliness levels in the vivarium.
Standard Rack Covers are available in six different • Washable in the cage / rack washer up to 180º F
colors to facilitate the segregation and easy • Rack Covers can be docked directly to bioBUBBLE
identification of components within the facility. Clean Rooms, isolators, and autoclave containers
Rack Covers easily slip onto your existing racks and for completely isolated transfer of clean materials
can be secured around the lowest shelf (optional). and animals. (optional)
Rack Covers are washable on an alkaline cycle at
temperatures up to 180º F.

www.bioBUBBLE.com | 33
bioBUBBLE has over
30 years experience
in creating custom ultra-
clean & containment
environments. We have
projects worldwide.
Big & small.

We have the solution to transform your

space efficiently & creatively.
Please contact us for more information >>
T 970 224 4262 | sales@biobubble.com

www.bioBUBBLE.com

34
970 224 4262 sales@ bioBUBBLE.com

www.bioBUBBLE.com | 35