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mono
Triglycerides Iiquicolor Manual calculation of the Triglycerides Concentration
Conversion factor: 1 mg/dl = 0.0114 mmol/l
GPO-PAP Method with Lipid Clearing Factor A sample
(LCF) C = [STD] x  [mg/dl; mmol/l]
A [STD]
Package Size
[REF] 10720P 9 x 15 ml Complete Test Kit Performance Characteristics
Linearity
10724 4 x 100 ml Complete Test Kit
The test is linear up to a triglyceride concentration of 1000 mg/dI or
10725 3 x 250 mI Complete Test Kit
11.4 mmol/l. Samples with a higher concentration have to be diluted 1 + 4
[IVD] with physiological saline (0.9%) and retested. Multiply the result by 5.
Method Typical performance date can be found in the Verification Report, acces-
The triglycerides are determined after enzymatic hydrolysis with lipases. sible via
Indicator is quinoneimine formed from hydrogen peroxide, 4-amino- www.human.de/data/gb/vr/su-trimr.pdf or
antipyrine and 4-chlorophenol under the catalytic influence of peroxidase. www.human-de.com/data/gb/vr/su-trimr.pdf
Reaction Principle If the performance data are not accessible via internet, they can be
lipases obtained free of charge from your local distributor.
Triglycerides  glycerol + fatty acids
Clinical Interpretation for Atherosclerotic Risk
GK Suspect: over 150 mg/dI or 1.71 mmoI/l
Glycerol + ATP  gIyceroI-3-phosphate + ADP
Increased: over 200 mg/dI or 2.28 mmol/l
GPO
GIyceroI-3-phosphate + O2  dihydroxyacetone phosphate + H2O2 QuaIity Control
POD All control sera with triglycerides values determined by this method can
H2O2 + 4-aminoantipyrine  quinoneimine + HCl + H2O be employed.
+ 4-chlorophenol We recommend the use our HUMATROL control sera based on animal
serum or our SERODOS based on human serum.
Contents
[RGT] 15 ml; 100 ml or 250 ml Monoreagent (R1) Automation
Proposals to apply the reagents on analyzers are available on request.
PIPES buffer (pH 7.5) 50 mmol/l
Each laboratory has to validate the application in its own responsibility.
4-chlorophenol 5 mmol/l
4-aminophenazone 0.25 mmol/l Notes
Magnesium ions 4.5 mmol/l 1. To correct for free glycerol, subtract 10 mg/dl (0.11 mmol/l) from the
ATP 2 mmol/l triglycerides value calculated.
Lipases  1300 U/I
2. The test is not influenced by hemoglobin values up to 150 mg/dl or by
Peroxidase  500 U/I
bilirubin values up to 40 mg/dl. Ascorbate may give falsely low values
Glycerol kinase  400 U/I
at > 4 mg/dl.
GIyceroI-3-phosphate oxidase  1500 U/I
Sodium azide 0,05 % 3. The reagents contain sodium azide (0.05%) as preservative. Do not
swallow. Avoid contact with skin and mucous membranes.
[STD] 3 ml Standard
4. False low Triglyceride results may possibly occur with samples from
Triglycerides see label
patients treated with N-acetyl cysteine (NAC, treatment of paracetamol
Reagent Preparation and Stability overdose), N-acetyl-p-benzoquinone imine and/or Metamizole. Blood
[RGT]/R1 and [STD] are ready for use. sampling should be performed before administration of metamizol.
The reagents are stable, even after opening, up to the stated expiry date Precautionary statements
when stored at 2...8°C. At 20...25°C the [RGT] is stable for 4 weeks.
P234 Keep only in original container.
Avoid contamination and freezing.
P260 Do not breathe dust/fume/gas/mist/vapours/spray.
Protect from light.
P262 Do not get in eyes, on skin, or on clothing.
Specimen P281 Use personal protective equipment as required.
Serum, heparinised plasma or EDTA plasma
P303+P361+P353 IF ON SKIN (or hair): Take off immediately all
Stability: 3 days at 2...8°C contaminated clothing. Rinse skin with water/shower.
4 months at -20°C P305+P351+P338 IF IN EYES: Rinse cautiously with water for several
Note: Lipemic specimens usually generate turbidity of the sample minutes. Remove contact lenses, if present and easy to do. Continue
reagent mixture which leads to falsely elevated results. The rinsing.
TRIGLYCERIDES liquicolor mono test avoids these falsely ele- P337+P313 If eye irritation persists: Get medical advice/attention.
vated results through its built-in Lipid-Clearing Factor (LCF).
P401 Store in accordance with local/regional/national/international
The LCF clears up totally a turbidity caused by lipemic
regulations.
specimens.
P501 Dispose of contents/container in accordance with
Assay local/regional/national/international regulations
Wavelength: 500 nm, Hg 546 nm
References
Optical path: 1 cm
1. Schettler, G., Nüssel, E., Arb. Med. Soz. Med. Präv. Med. 10, 25 (1975)
Temperature: 20...25°C or 37°C
2. Jacobs, N. J., VanDemark, P. J., Arch. Biochem. Biophys. 88, 250-255
Measurement: against reagent blank (RB). Only one reagent blank per (1960)
series is required.
3. Koditschek, L. K., Umbreit, W. W., J. Bacteriol. 68, 1063-1068 (1969)
Pipetting Scheme 4. Trinder, P., Ann. Clin. Biochem. 6, 24-27 (1969)
Please use the triglyceride standard included in the test kits, or AUTOCAL
[REF] 13160 for automated procedures.
Pipette into cuvettes RB Sample or [STD]
SU-TRIMR INF 1072401 GB 12-2021-17 |
Sample / [STD] --- 10 μl
[RGT]/R1 1000 μl 1000 μl
Mix and incubate for 10 min. at 20...25°C or for 5 min. at 37°C. Measure
the absorbance of the sample (A sample) and the standard (A [STD])
against the reagent blank within 60 min.