GLUCOSE

(GOD / POD METHOD)

INTENDED USE:
This reagent kit is intended for the "in vitro" quantitative
determination of Glucose in Serum / Plasma. PROCEDURE:
Pipette into clean dry test tubes labeled as Blank (B),
CLINICAL SIGNIFICANCE: Standard (S) and Test (T) :
Glucose is the major carbohydrate present in blood. Its oxidation in
the cells is the source of energy for the body. Increased levels of Addition Sequence B. S. T.
glucose are found in diabetes mellitus, hyperparathyroidism,
pancreatitis, renal failure. Decreased levels are found in Enzyme reagent 1 ml 1 ml 1 ml
Insulinoma, hypothyroidism, hypopituitarism and extensive liver Standard - 10 µl -
disease. Sample - - 10 µl

PRINCIPLE:
Glucose is oxidised to gluconic acid and hydrogen peroxide in the Mix well and incubate at 37°C for 10 mins. Measure
presence of glucose oxidase. Hydrogen peroxide further reacts absorbance of the Standard (Abs.S) and Test (Abs.T) against
with phenol and 4-aminoantipyrine by the catalytic action of Reagent Blank at 505 nm.
peroxidase to form a red coloured quinonimine dye complex.
Intensity of the colour formed is directly proportional to the amount CALCULATION :
of glucose present in the sample.

REACTION: Abs.T
Glucose mg/dl = X 100
Glucose Oxidase Abs. S
Glucose + O2+ H 2O Gluconic Acid + H2O2
NORMAL VALUE :
Peroxidase Fasting : 70 - 110 mg /dl
H 2O2+ Phenol + 4 aminoantipyrine quinonimine + H2 O PPBS : Up to 130 mg/dl
Each Laboratory should establish it's own normal
CONTENTS: range representing its patient population.
Reagent 1 : Glucose Enzyme Reagent
Reagent 2 : Glucose Standard 100 mg/dl LINEARITY :
Reagent 3: Glucose Standard 400 mg/dl (Free)
This procedure is linear upto 600 mg/dl. If values exceeds this
limit, dilute the serum with normal saline and multiply result by
MATERIALS REQUIRED BUT NOT PROVIDED:- dilution factor.
- Clean & Dry Glassware.
- Laboratory Glass Pipettes or Micropipettes & Tips. QUALITY CONTROL :
- Colorimeter or Bio-Chemistry Analyzer. For accuracy it is necessary to run known controls with
every assay.
SAMPLES:
Blood should be collected in a clean dry container. Serum or LIMITATION & PRECAUTIONS :
plasma should be seperated from the cells at the earliest possible 1. Storage conditions as mentioned on the kit to be adhered.
(within 30 minutes), as the rate of glycolysis is approximately 7 2. Do not freeze or expose the reagents to high temperature
mg% per hour at room temperature. Sodium flouride is preferred as and protect from direct sun light as it may affect
anticoagulant due to its antiglycolytic activity. The higher
the performance of the kit.
concentration of sodium flouride, more than 10 mg/dl blood should
be avoided as it may inhibit the colour development. Glucose is 3. The Enzyme reagent contains Sodium Azide.
stable for 24 hours in neatly seperated plasma and serum. If the 4. Avoid contamination of the reagent during the assay
estimation is not possible within 24 hours then the specimen should process.
be preserved at -10° C and should be used within 30 days. 5. Before the assay bring all the reagents to room
temperature.
PREPARATION OF REAGENT & STABILITY : 6. Use clean glassware free from dust or debris.
All Reagents are ready to use and are stable till the expiry date 7. Re-plug the Glucose standard vial after use.
mentioned on the label, when stored at 2 - 8° C.
BIBLIOGRAPHY :
GENERAL SYSTEM PARAMETERS: 1. Barham,D, and Trinder, P. Analyst 1972 : 97:142.
Reaction type : End point 2. Tenscher, A. and Richterich, P. Schweiz Med. Wschr.
Wave length : 505 nm (490-530 nm) 1971: 101:345 and 390
Temperature : 37°C CODE NO. PACK SIZE Reagent 1 Reagent 2 Reagent 3
Incubation : 10 minutes S07 3 x 100 ml 3 x 100 ml 5.0 ml 5.0 ml
Reagent volume : 1.0 ml S07A 6 x 100 ml 6 x 100 ml 5.0 ml 5.0 ml
Sample volume : 10 µl S07B 2 x 500 ml 2 x 500 ml 5.0 ml 5.0 ml
Standard concentration : 100 mg/dl
Zero setting : Reagent blank IVD
UNIONPROJET S.R.L.S.
Light path : 1.0 cm EC REP Via Aliprandi 41-20851
Lissone (MB)
www.jas-anz.com.au/register

ISO 9001:2015 ISO 13485:2003