POTASSIUM

(Monotest)
INTENDED USE :
This reagent kit is intended for the “In Vitro “ quantitative
determination of Potassium in Serum. LINEARITY :
This procedure is linear upto 7 mEq/L. If values exceed
PRINCIPLE: his limit dilute the sample with distilled water and multiply
Potassium reacts with sodium tetraphenol boron in a specially results with proper dilution factor.
prepared buffer to form a colloidal suspension. The amount of the
turbidity produced is directly proportional to the concentration of NOTE :
potassium in the sample. As potassium is a very widely distributed ion, care should be
taken to avoid any contamination. All g lasswares being used
CONTENTS : for the test should fisr t be rinsed wth
i 1% or 0.1 N HNO3 and
Reagent 1 : Potassium Reagent then with good quality deionised water before use.
Reagent 2 : Potassium Standard 5 mEq/L
BIBILOGRAPHY:
SAMPLE COLLECTION AND PRESERVATION : 1. Tietz, N.W., Fundamentals of Clinical Chemistry,W.b.
Serum free from hemolysis. Saunders Co. Phila, P.A. p. 874.
Separate serum from the clot as soon as possible as 2. Henry R.F., et, al, Clinical Chemistry Princi ples a nd Technics.
potassium may leach from the RBC’s which can elevate 2ndE d, H arper an d R ow, Harper and Row, Hargersein, M.D.
pottasium level. (1974)
3. Maruna RFL., Clin Chem. Acta. 2:581, (1958)
4. Trinder, P:Analyst, 76:596, (1951)
REAGENT PREPARATION AND STORAGE :
All reagents are ready to use. Store reagents at Room
Temperature (25 - 30°C). Code No. Pack Size Reagent 1 Reagent 2
S21 25T 25 x 1.0 ml 3.0 ml
REAGENT STABILITY :
The reagents are stable until the expiration date mentioned on the IVD
kit.
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GENERAL SYSTEM PARAMETERS :

Reaction type : End point
Wavelength : 630 nm
Cuvette : 1 cm
Reaction Temperature : RT
Zero setting : Reagent blank
Reagent volume : 1.0 ml
Sample volume : 20 µl
Incubation time : 5 mins
Standard concentration : 5 mEq/L

ASSAY PROCEDURE :
Pipette into clean dry testtubes labeled as Blank (B), Standard
(S), and Test (T) :

Addition Sequence (B) (S) (T)

Potassium reagent 1.0 ml 1.0 ml 1.0 ml
Standard - 20 µl -
Sample - - 20 µl

Mix well and incubate at RT for 5 mins. Measure the absorbance

of Standard (Abs.S) and Test (Abs. T) against reagent blank at
630 nm.

CALCULATION :
Concentartion of Potassium (mEq/L) : Abs. T
X5
Abs. S

QUALITY CONTROL :
It is recommended that controls be included in each set of assays.

NORMAL VALUES :
Serum/Plasma : 3.5 - 5.5 mEq/L.
It is recommended that each laboratory establish its own normal
range representing its patient population.