Professional Documents
Culture Documents
CONTENTS
COPYRIGHT AND DECLARATION........................................................................................... I
CHAPTER 1 INTRODUCTION....................................................................................................1
CHAPTER 2 PRECAUTIONS.......................................................................................................9
CHAPTER 6 TEST........................................................................................................................33
II
Copyright and Declaration
We owns the copyright of this unpublicized issued manual, and has right to handle as
secret information. This manual just used as reference for operation, maintenance and
service of our product. Other personnel have no right to publish this manual.
This manual includes special information protected by copyright law. Copyright
reserved, prohibit copy and transmit any content of this manual against not through written
agreement by us.
We don’t make any formally guarantee for this manual, including (but not limit to)
implied guarantee responsibility on marketability and propriety lodged for certain purpose.
We without responsibility for the error included in this manual and indirectly & abiogenetic
damage that is caused by actual representation & usage provided by this manual.
Content in the manual can be changed without giving notice.
Applicable models: BONAVERA
- 5R
Our obligation
We only responsible for instrument security, reliability and capability under following
condition
Performed assemble, extend, re-debugging, improve and repair by our authorized
personnel.
Relevant wiring equipments accord with national standard.
Use the analyzer according to this service manual.
NOTE
This analyzer cannot be used in family.
WARNING
If each hospital or institution that is responsible for using this instrument cannot realize
a set of satisfactory service procedure, will cause deviant invalidation of instrument, even
jeopardize to health of human body.
Nowadays, we provide relevant technical information conditionally when customer
request. In addition, narrate calibration method and other information through list to help
eligible technician to repair our instrument.
I
Copyright and Declaration
Guarantee
Manufacturer techniques and material
We guarantee that the automated hematology analyzer has no techniques and
material problems within one year from shippingday if under normal use and maintenance.
Free service
Our obligation under this guarantee not include freight and other fare, not responsible
for direct, indirect and ultimate damage & delay caused by following condition: misuse,
replaced accessories or repaired by personnel not authorized by us.
This guarantee is not applicable for following items: Improper use; Without
maintenance or damaged machine; BONAVERA 5R original serial number label or
manufacturer logo has been replaced or torn off; Other manufacturers’ products.
You should get the right of return first, please contact the BONAVERA 5R sales
company with product series number which marked on nameplate, we will not accept if S/N
cannot beidentified. Please mark the instrument No., S/N and why you return the product.
Freight: if send back instrument for service, purchaser bears the freight (including
custom fare)
Version: 02/2017-C2
II
Chapter 1 Introduction
1.1 Front View
Indicator light
1
Chapter 1 Introduction
Cooling fan
Reagent
interfaces
2
Chapter 1 Introduction
USB
Internet access
Reserved interface
Serial port
Power switch
AC power plug
Grounding column
BONAVERA 5R achieves WBC differential count with Multi-angle laser light scattering
technique and obtains the blood cell analysis via three independent detection channels.
1) WBC/DIFF channel: achieves WBC count and classification with laser light
scattering technology in the sheath flow regulator. Complete WBC count and
classification in one channel.
2) WBC/HGB channel: WBC testing by electrical impedance method, and
hemoglobin testing by colorimetry.
3) RBC/PLT channel: RBC and PLT counting by Electrical impedance
Electrical impedance of white blood cells (WBC) count principle which is based on the
principle of non-conductive causes resistance change when blood cell granules in diluents
go through the aperture. Take it as the basis for testing to count WBC and determine its
volume.
3
Chapter 1 Introduction
Counting chamber
External electrodes
Internal electrodes
Outer chamber
Inner chamber
Inner and outer electrodes are placed inside and outside the room in the counting
chamber. The two chambers are separated by a ruby aperture with a diameter of 100μm.
The rear chamber is filled with a certain concentration of cell suspension, and the front
chamber is filled with diluents.
The cell’s conductivity which is lower than diluent’s conductivity, so is the relative poor
conductor. When a cell granule in front chamber goes through the aperture, it generates
an instantaneous pulse voltage between inner and outer electrodes. The number of pulses
is proportional to the number of cells. Pulse height is proportional to the size of the cell
volume. Under the influence of negative pressure, a certain capacity of the cells will
continue through the aperture, thereby generating a series of pulses. Send to count for
obtaining a certain volume of total cells by pulse signals amplification, threshold adjustment,
identification, shaping and A / D conversion. (See Figure 1-4)
BONAVERA 5R not only calculates the overall amount of WBC, but also offers graphics
leukocyte distribution - the scatter plot.
4
Chapter 1 Introduction
5
Chapter 1 Introduction
6
Chapter 1 Introduction
The detection principle of RBC is the same as that of WBC. In RBC count chamber,
cells arranged in a certain capacity go through aperture (68μm) under the negative
pressure. Pulse is formed during this process. The total number and average volume of
RBC are obtained according to pulse size and height. The RBC volume distribution
histogram is shown in Figure 1-8.
Normally, ratio of number of RBC and WBC is approximately 750:1, so it can ignore
factors caused by WBC as testing the RBC. However, in some special pathological
conditions, such as leukemia simultaneously with blood disease, may cause abnormal
RBC count.
HCT=(MCV × RBC) /10. According to the relevant algorithm, the MCH and MCHC can
be derived by RBC, MCV and HGB. RDW is obtained as testing RBC number and volume
differences, which reflects the outer periphery of RBC volume heterogeneity. RDW which
reflects the extent of RBC sizes, which has clinical significance for diagnosis of anemia.
7
Chapter 1 Introduction
Platelet (PLT) and RBC are tested in the same counting chamber. The analyzer
respectively counts it according to different thresholds. (See Figure 1-9)
PLT data stores in 64 channels within the range of 2fL to 30fL.
Hemoglobin (HGB) and WIC are tested in the same cup. Lyse destroys RBC and the
HGB is dissolved out. Related ingredients combine to form an HGB complex.
Colorimetric assay in specific wavelength (540nm) in counting chamber, absorbance
change is proportional to HGB content in liquid. HGB test results are obtained by
correlation algorithm.
8
Chapter 2 Precautions
2.1 External Factors
2.1.1 Voltage
To ensure the normal work and stable test, the analyzer uses 220V power input. High-
precision automatic AC power supply should be installed as the electric supply is unstable.
If intermittent power outages happen frequently, please install the UPS uninterruptible
power supply, so as to avoid damage to the power and circuit board.
Acquisition signal is very weak, external interference may cause abnormal data.
Therefore, it’s recommended connecting with ground wire to avoid affecting the test results
by interference signal. Away from the equipments generated interference signals, such as
monitors, copiers, centrifuges and X-ray detector.
2.1.3 Temperature
1. Place the analyzer and reagents in the same horizontal plane to ensure reagent
can be quickly added into the analyzer.
2. Waste containers should be placed on the ground. (Avoid waste overflowing)
3. Insert the reagent connectors. Diluents connect with the blue one, lyse connects
with the red one, detergent connects with the green one and sheath connects with
the yellow one.
1. Check whether the tubing connector of flow system looses or cracks. If so, please
deal with it before boot.
2. After boot, check whether there’s abnormal sound or smell, the screen display is
normal or not. If so, please shut down the analyzer immediately and check it.
9
Chapter 2 Precautions
3. Check whether the screen display and program initialization is normal. Enter
sample test interface if it’s normal.
There are two sample test modes, which are whole blood and diluent.
1. Whole blood sampling: collecting human blood by vacuum blood collection tube.
The anticoagulant in the collection tube anticoagulats the blood sample.
2. Diluent sampling: collecting human peripheral blood with blood collection tube,
such as fingers, ears and so on.
3. Whole blood test: in Test interface, put the test sample under the aspiration probe
and then press count button to test.
4. Diluent test: put the disposable test tube under the aspiration probe and click
“Drain” to automatically discharge 500μl diluent into the test tube. Collect 20μL
peripheral blood and mix it in the test tube. Select “Diluent” mode in test interface,
put this tube under the aspiration probe and press count button on the front
housing. The analyzer starts to test.
※Note: avoid squeezing when collecting peripheral blood so as not to extrude tissue
fluid or aggregate PLT, which may affects PLT counting. Needle goes a little bit
deeper when collecting peripheral blood. Do not collect first drop of blood as
sample.
10
Chapter 3 Circuit
The circuit consists of switch mode power supply (SMPS), ARM(A8)_FPGA, OMC
board, PreAMP board, MVDriver, JWLM board, AnalogPower board, LMS board, Light
panels, LED_LOCK board, Front housing Keyboard, card reader and Monitor driver.
3.1 Introduction
Impedance-
motordrive
board
Impedance-
PreAMP
board
Optical
module
control board
11
Chapter 3 Circuit
LMS board
JWLM
board
12
Chapter 3 Circuit
A8 AMP
Board
AnalogPower
board
A8 core
switch mode
power supply
(SMPS)
13
Chapter 3 Circuit
monitor
Driver
Front housing
Keyboard
Front
housing
card reader
Keyboar
d
ARM (A8)_FPGA which is the control center of the analyzer stores gain and motor
steps. LED1 blinks as the A8 AMP board working. See Figure 3-5.
14
Chapter 3 Circuit
Display power
A8 core
interface
Interface of
JWLM board
Interface of LMS board power supply CAN interface SD-TF Card slot
input (connect with
OMC)
Figure3-5 ARM (A8)_FPGA
Analog Power board which is responsible for system logic control provides various
parameters and executes the command. See Figure3-9.
Analog power board which is responsible for provides the ±12V analog power supply
to PreAmp board and OMC board, at the same time also provides the 5V power supply to
optical laser and +12V to fan interface. As shown in Figure 3-6.
15
Chapter 3 Circuit
+/-12V
+-12V power
电源
interface*2
接口*2
5V power
5V 电源接口
interface*2
*2
Fan +/-12V
风扇+12V 电 Power supply,
power 开 关电源 ,
源接口 power
电源输入 input
interface*2
PreAmp board, which amplifies and processes weak cellular signal of transducers,
monitor the vacuum of impedance flow system’s negative pressure tank and current
ambient temperature.
16
Chapter 3 Circuit
12V analog
power input
ARM
(A8)_FPGA
interface
Interface of
impedance
negative
pressure tank
Temperature
Sensor interface
HGB interface
Interface of
WBC interface
RBC and PLT
It’s mainly used to control the optical counting liquid circuit, analyze optical signal and
upload optical signal to ARM (A8)_FPGA. See Figure 3-8.
17
Chapter 3 Circuit
电机 MH 接
MH interface
口
V31
电磁阀 V31
signal interface
90°d 信号 4 V39 V39
电磁阀
接口
signal interface
90°信 号接 3
口
signal interface
10°信 号接 2
口
0 ° 信 号1 接 V38
signal interface 电磁阀 V38
口
V46 V46
电磁阀
MH Optocoupler
MH 光 耦 接
interface口(KH)
(KH) 光学鞘液罐
Float sensor interface of
的浮子传感
MG MG 光 耦 接
Optocoupler optics sheath
器接口 tank
口(KG)
interface (KG)
Figure3-8 OMC Board
NOTE:
1. Pressure detection pipeline of optics sheath tank shall connect to the interface
under the pressure sensor.
2. Take AGND as the reference as testing the voltage of S0, S10, S90 and S90D.
(black probe of oscilloscope connects to AGND)
3. The voltage of S0, S10, S90 and S90D is magnified as these test points passing
through AMP module of OMC board. It’s not the original signal output via signal
acquisition board, so inappropriate gain causes signal distortion.
18
Chapter 3 Circuit
19
Chapter 3 Circuit
It mainly used to check reagents (diluent, lyse and sheath) and level detection of
diluent reservoir. See Figure 3-10.
Interface of
JWLM board
3.1.8 MV Driver
MV Driver which is responsible for each motor motion in impedance flow system and
for turning on and off solenoid valve pump.(See Figure3-11)
Please see the label for the interface of MV optocoupler. The corresponding
optocoupler of MA, MB, MC and MD is KA, KB, KC and KD.
NOTE: Please make sure the jumper cap is in the correct installation state.
20
Chapter 3 Circuit
Power switch
开 关 电 13V,
源 Install the jumper
24V input
13V,24V 输 cap,确保跳线帽以安
otherwise the
入 optocoupler
装,否则光耦无 cannot
法正常工作
work normally.
Interface of A8
motor接 A8 主板的
solenoid
电 机电磁 阀
valve
接口
Figure3-11 MV Driver
21
Chapter 3 Circuit
The test module for counting time consists of an LMS board and two glass tubes.
There are 4 optocouplers and 4 potentiometers. 4 optocouplers corresponds to TEST1-
TEST4 test points. The voltage is 4.8±0.2V when there’s liquid in the glass tubes. The
voltage is 2.9±0.1V when there’s no liquid in the glass tubes. The voltage shall shift
because of optocoupler parameter offset and dirty glass tube.
The LMS board calculates the inhaled liquid by optocoupler and the metering tube,
which ensures the measurement accuracy of WBC, RBC and PLT. There are two channels
in LMS board, one is for WBC, the other is for RBC and PLT. Each channel consists of one
measuring tube and two optocouplers. Open the valve which controls air, the air goes into
the WBC measuring tube and RBC measuring tube. Empty liquid in the tube. Close the air
control valve as test starting. Liquid in the counting chamber goes through aperture and
flows to measuring tube. Liquid in the transducer goes through measuring tube, the liquid
column is gradually moving down. The comparator output counts start signal as liquid
column through the upper optocoupler. The comparator output stops counting start signal
as liquid column through the down optocoupler.
NOTE: as there no liquid in the glass tubes, the test point voltage TEST1-TEST4 should
be adjusted within the range DC2.9V ± 0.1V, otherwise it may give a false alarm of clogging,
bubbles or no reagents.
22
Chapter 4 Flow System
4.1 Position of Key Components
MD of sampling
采样机构纵 MC of sampling
unit
向电机 MD。 采 样针 横向 Glass
计数玻璃管tubes
unit
电机 MC。
Lyse 溶血剂注射
syringe, Diluents
稀释液注射 syringe,
corresponding
器,对应 MA to corresponding
器,对应 MA to
MA 电机 MA
电机。
Sampling syringe,
采 样 注 射
corresponding
器,对应电 to MB
机 MB
23
Chapter 4 Flow System
Optical sheath
reservoir
Optical pressure
tank
24
Chapter 4 Flow System
Cleaning set
Aspiration
Optical 10mL sheath probe
syringe(MG)
25
Chapter 4 Flow System
Impedance
negative pressure tank
4.3 Transducers
As shown in Figure 4-1 transducer components which is the counting sensor of the
analyzer is the most front-end detection element of data acquisition.
WOC transducer is used to mix liquid.
Measuring RBC, WBC and PLT parameters via Coulter principle (electrical impedance
principle). In transducers, the circuit provides a constant current through diluted conductive
liquid in cell counting. As cells go through aperture, the loop resistance changes. Cells with
different volume produce electrical pulses with different amplitudes, so cells volume and
numbers can be calculated.
Make a Colorimetric analysis towards the treated sample and calculate HGB value via
light emitting and receiving of WBC transducer.
NOTE: the liquid should be sprayed on the walls of the transducer, or the results of
MCV, PLT and HGB shall be affected.
27
Chapter 4 Flow System
5160液路原理图
Optical Module
V31 光学模块
NC
V34 U1
NC NO
C V35
SHEATH
NO NC V36 NC
V32 蓝 绿 C
NC Blue Green WOC
红
黄 10ml
Red 储液罐 正压罐 V40 V44
yellow V37 NC
Liquid storage pot Positive pot C
NC NO
MG
C
V33 500ul NC NO
V38 V46
NC V39
MH
NC WASTE
Impedance Module
阻抗模块
NC
C V2 V18
V5 V4 V17
C NO NO
C NC NC
NO NC
NC
V1
NC NO
V3
C
NC
C
WBC RBC NO
V7 V8
NC NC
MB MA
计数真空罐
MD V16 V15
NC NC
LYSE DILUENT
Vacuum pump
V12 V11
NC NC
V6
NO NC
C V20
DETERGENT
V9
NC
V10 V19
NC WASTE
V31 光学模块
Optical Module
NC
V34 U1
NC NO
C V35
SHEATH
NO NC V36 NC
V32 蓝
Blue 绿
green C
NC
WOC
红
Red
Yellow黄 10ml
储液罐 正压罐 V40 V44
V37 NC
C
NC NO
MG
Liquid storage pot Positive pot
C
V33 500ul NC NO
V38 V46
NC V39
MH
NC WASTE
28
Chapter 4 Flow System
Add 1400μL sheath into WOC cup by MG, use MB syringe to collect 28μL blood and
inject 18μL of it into WOC cup. MG sends sheath into WOC cup via V34, V35 and V37,
V44 mixes blood and sheath via V40 and V39. Open the V34, V35 and V37, pump the
mixed liquid into the channel between V34 and V37 by MG motor. The sheath is pushed
by 160Kpa pressure to flow through V32 and into sheath flow regulator. It forms a sheath
flow and wraps cells. Cells are in queue status. MH makes cells move upwards one by one.
Cell sorting was performed under laser irradiation. The waste is pumped and discharged
by V46.
Impedance Module
Vacuum pump
30
Chapter 5 Optical System
5.1 Optical Structure
31
Chapter 5 Optical System
32
Chapter 6 Test
6.1 Motor and Valve Test
Click “Service” in test interface, input “1111” and click “OK” to enter valve test interface.
Click “Pre page”, “Next page” to switch valve test, motor test, system state test1 and
system test 2.
33
Chapter 6 Test
Click “Service” in test interface, input “5555” and click “OK” to enter the interface. Click
“Pre page”, “Next page” to select Motor parameter, Optical parameter and gain parameter.
“MC2” represents the steps of sampling traverse motor from WIC cup to RBC
transducer. It requests the aspiration probe to fall in the middle of RBC transducer.
“MC3” represents the steps of sampling traverse motor from WOC cup to WIC cup. It
requests the aspiration probe to fall in the middle of WBC transducer.
Descriptions of other parameter:
Discharge
Parameter Description Default
amount
Downward movement distance of aspiration probe in
“MD1”
standby station after counting
“MD2” Distance of moving the aspiration probe to WOC cup
“MD3” Distance of moving the aspiration probe to WIC cup
“MD4” Distance of moving the aspiration probe to RBC cup
Steps of sampling traverse motor from sampling position to
“MC1”
WOC cup
“MC2” Steps of sampling traverse motor from WIC to RBC cup
“MC3” Steps of sampling traverse motor from WOC to WIC cup
Sampling amount in whole blood mode (external)
“MB1”
(CBC+DIFF)
“MB2” Sampling amount in diluent mode (external) (CBC+DIFF)
“MB3” Sample amount in WOC cup in whole blood mode
“MB4” Sample amount in WOC cup in diluent mode
“MB5” Sampling amount in whole blood mode (external) (CBC)
“MB6” WBC=>RBC sampling amount (whole blood CBC+DIFF)
“MB7” WBC=>RBC sampling amount (whole blood CBC)
“MB8” WBC=>RBC sampling amount (diluent CBC+DIFF)
“MB9” WBC=>RBC sampling amount (diluent CBC)
“MA1” Addition amount of lyse (whole blood CBC+DIFF)
“MA2” Addition amount of lyse (diluent CBC+DIFF)
“MA3” Unused
Addition amount of diluent (whole blood CBC+DIFF) WBC
“MA4”
cup
Addition amount of diluent (whole blood CBC+DIFF) RBC
“MA5”
cup
“MA6” Addition amount of diluent (diluent CBC+DIFF) WBC cup
“MA7” Addition amount of diluent (diluent CBC+DIFF) RBC cup
“MA8” Unused
“MA9” Discharge amount in diluent mode 500uL
“MA10” Addition amount of lyse (whole blood CBC)
“MA11” Addition amount of lyse (diluent CBC)
“MA12” Addition amount of diluent (whole blood CBC) WBC cup
36
Chapter 6 Test
sample
WOC sheath addition
MG_STEP2 0--65535
amount in diluent mode
Motor steps of
controlling WOC
MG_STEP3 0--65535
sampling amount in
diluent mode
Motor steps of
controlling WOC
MG_STEP4 0--65535
sampling amount in
whole blood mode
WOC sample counting
MH_STEP1 steps in whole blood 0--65535
mode
WOC sample counting
MH_STEP2 0--65535
steps in diluent mode
MH_STEP3 Undefined
MH_STEP4 Undefined
WOC mixing delay
MG_FRQ1 adjustment in whole 0--65535
blood mode
WOC mixing delay
MG_FRQ2 adjustment in diluent 0--65535
mode
MH_FRQ1 Undefined
Adjustment of WOC Recommended values: 4000
MH_FRQ2 0--65535
reaction time (T=4000*2*5000/(10E-7)=4s)
Debugging
dedicated, 0xAABB,AA is the duration of high
Optical ADC sampling do not level of the sampling square wave,
ADFRQ
rate setting change it. BB the duration of low level(unit is
(default 0.01uS)
65535: 4M)
Blank voltage VB= BACKMIN/2 mV
Only the sampling points which are
Blank voltage of optical
BACKMIN 0--8191 higher than VB shall be processed.
signal 1
Only those points shall be included
in the pulse width.
The pulse width which is greater
Minimum pulse width
WIDTHMIN 0--65535 than the value is considered a cell
setup
signal.
The pulse width which is smaller
Maximum pulse width
WIDTHMAX 0--65535 than the value is considered a cell
setup
signal.
38
Chapter 6 Test
6.2.3 Impedance Gain Adjustment (WIC, RBC, PLT, HGB and Vacuum degree)
Check the gain of RBC, WIC and PLT, check HGB blank voltage and check vacuum
degree after testing by control material.
➢ WBC
Click “Setup”-“Service”, input “6666” and choose to open WIC histogram in popup
dialog box. As shown in Figure 6-7, the abscissa of the second crest is got.
39
Chapter 6 Test
Figure6-8 RBC
➢ PLT
Click relevant figure to enter the interface.
Move the coordinate line to the crest position to get the PLT coordinate value.
Figure6-9 PLT
40
Chapter 6 Test
➢ Insert the prepared SD card into the SD card slot. For the position of slot, please
see figure 6-13.
41
Chapter 6 Test
➢ Push “1” of SW5 upwards and push “2” of SW5 downwards. See Figure 6-11.
➢ Push “1” of SW2 upwards
➢ Turn off the power for 5 seconds and turn on it again. It enters program upgrading
interface.
➢ Remove the SD card as seeing “the upgrade is completed”.
➢ Turn off the power, reset SW5 and SW2. Turn on the power after 5 seconds.
Successful boot means successful program upgrading.
Figure6-11 SW5
Figure6-12 SW2
42
Chapter 6 Test
SD card slot
Long press the count button on the front housing to access main interface as seeing
in the upper left corner of the screen. Use this method when maintaining on the
client side.
43
Chapter 7 Troubleshooting
7.1 Optical Troubleshooting
Wrong optical classification, it cannot be clearly classified the blood sample to 3 cell
populations, or scatters only appear in a straight line on S0 image.
Click “Setup”-“Service” , input “2006” and click “S0Back” to see optics signal 1 (0°)
blank voltage. If the blank voltage is greater than 2.5V, it preliminarily considered that the
sheath flow regulator is dirty. There are two conditions, which are dirty inner wall and dirty
outer wall. Wipe around the sheath flow regulator with a clean cloth and then check the
S0 blank voltage. If it is less than or equal to 2.5V, it represents the outer wall is dirty.
Make a fresh blood sample test; if the results are meets requirements, problem is solved.
If the S0 blank voltage doesn’t change, it is considered that the inner wall is dirty. Cleaning
method: click “Setup”-“Service”, put the detergent under the aspiration probe and click
“Soak sheath flow regulator”. Make two blank tests after soaking and check the S0 blank
voltage. If it is less than or equal to 2.5V, please make a fresh blood sample test.
44
45