UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT

COLLEGE OF HORTICULTURE, BENGALURU

Course Teacher: Dr. Manjunath Ramanna, Assistant Professor of Agricultural Microbiology

Title of the course: Fundamentals of Microbiology, Course No.: AMB 101 Credit Hours: 2(1+1)

Chapter Syllabus Course outline(Theory) No. of Remarks

No. classes
1. History and scope The discovery of Microorganisms, Spontaneous generation conflict, 1
of Microbiology Germ theory of diseases, Development of Microbiology in India
2. Different groups of
Prokaryotes and Eukaryotes, Prokaryotic cell structure and function 2
microorganims and
their characteristics Internal and external cell organells of bacteria
3. Microscopy Basics of Microscopy: Numerical Aperture, Resolving Power, Types 2
of Microscopes, Bright Field Microscope, Dark field Microscope,
Phase contrast Microscope, Electron microscope, etc
4. Specimen Dyes and Stains, Simple staining, Differential staining, Grams’ 2
preparation staining, Endospore staining, flagella staining, Acid fast staining
Mid-Term Exam
5. Microbial growth Types of culture media, Growth of bacteria, fungi and their growth 2
media measurements, Microbial growth curve, Turbidostat, Fermentors
6. Sterilization Physical: Heat and irradiation and chemical methods(Liquid and gas), 2
techniques Pasteurization,
7. Pure-culture Isolation or preparation of pure cultures and preservation of microbial 2
techniques cultures
8. Viruses General characteristics of virus and brief description of 2
Bacteriophages, diseases, Prions, Viroids, Virion, Growth of virus
9. Industrial 2
applications of Enzymes, Antibiotics, Alcohols, Organic acids, etc
microorganisms
10. Mushrooms Edible and poisonous types, nutritive values, culturing and production 2
technique of Oyster and Button mushrooms
11. Revision 1

Sl. No. Practical syllabus No. of classes

1. Sterilization techniques 2
2. Preparation of microbial culture media 2
3. Examination of natural samples for microorganisms 2
4. Isolation of bacteria, fungi and yeasts 2
5. Serial dilution plate count techniques 2
6. Isolation of bacteriphages (plaque forming units) 2
7. Preparation of culture broths, agar slants 2
8. Pure culture techniques 2
9. Turbidometric estimation of microbial growth 2
10. Mushroom culture: spawn production, culture and production 2
techniques, harvesting, packing and storage techniques
11. Practical Examination 1
.
 UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT
COLLEGE OF HORTICULTURE, BENGALURU

Course Teacher: Dr. Manjunath Ramanna, Assistant Professor of Agricultural Microbiology

Title of the course: Fundamentals of Microbiology, Course No.: AMB 101 Credit Hours: 2(1+1)

Chapter Syllabus Course outline(Theory) No. of Remarks

No. classes
12. History and scope The discovery of Microorganisms, Spontaneous generation conflict, 2
of Microbiology Germ theory of diseases, Development of Microbiology in India
13. Different groups of 2
microorganims and Prokaryotes and Eukaryotes, Prokaryotic cell structure and function
their characteristics
14. Microscopy Basics of Microscopy: Numerical Aperture, Resolving Power, Types 2
of Microscopes, Bright Field Microscopy,
15. Specimen Dyes and Stains, Simple staining, Differential staining, Grams’ 2
preparation staining
Mid-Term Exam
16. Microbial growth Types of culture media, Growth of bacteria, fungi and their growth 2
media measurements, Microbial growth curve
17. Sterilization 2
Physical and chemical methods
techniques
18. Pure-culture Isolation or preparation of pure cultures and preservation of microbial 2
techniques cultures
19. Viruses General characteristics of virus and brief description of 2
Bacteriophages
20. Industrial 2
applications of Enzymes, Antibiotics, Alcohols, Organic acids, etc
microorganisms
21. Mushrooms Edible and poisonous types, nutritive values, culturing and production 2
technique of Oyster and Button mushrooms
22. Revision 1

Sl. No. Practical syllabus No. of classes

12. Sterilization techniques 2
13. Preparation of microbial culture media 2
14. Examination of natural samples for microorganisms 2
15. Isolation of bacteria, fungi and yeasts 2
16. Serial dilution plate count techniques 2
17. Isolation of bacteriphages (plaque forming units) 2
18. Preparation of culture broths, agar slants 2
19. Pure culture techniques 2
20. Turbidometric estimation of microbial growth 2
21. Mushroom culture: spawn production, culture and production 2
techniques, harvesting, packing and storage techniques
22. Practical Examination 1