NAME:____________________BLOCK:____DATE:________

Lab: Blood Types

Scenario:
Today was a busy day for our police force in Independence KY. The crazy
pioneer puppet struck 6 times today. At each of his crime scenes he or she
leaves behind a pioneer puppet. This individual is toying (ha, ha, ha) with the
fine citizens of Independence. The Independence police force is too busy to
handle all of the crime scene investigations. You know how to tell the difference
between different blood types, so the police will need your help. It is up to you to
help grab the pioneer puppet. Each crime scene group has been given a sample
of blood found at one of the 6 crime scenes. Each group has also been given
blood samples of 4 suspects who hate the Simon Kenton Pioneers

Materials:
 Crime scene blood sample
 Mr. Smith blood sample
 Mr. Jones blood sample
 Mr. Green blood sample
 Ms. Brown blood sample
 5 blood typing trays
 simulated anti-A typing serum
 simulated anti-B typing serum
 simulated anti-Rh typing serum
 toothpicks

Procedure:
1. Label each blood typing trays:
1. Crime Scene
2. Mr. Smith
3. Mr. Jones
4. Mr. Green
5. Ms. Brown
2. Place three drops of crime scene blood in each of the A, B, and Rh wells of
tray 1.
3. Place three drops of Mr. Smith’s blood in each of the A, B, and Rh wells of
tray 2.
4. Place three drops of Mr. Jones’s blood in each of the A, B, and Rh wells of
tray 3.
5. Place three drops of Mr. Green’s blood in each of the A, B, and Rh wells of
tray 4
6. Place three drops of Ms Brown’s blood in each of the A, B, and Rh wells of
tray 5
7. Add three drops of simulated anti-A serum in each A well on the 5 trays.
8. Add three drops of simulated anti-B serum in each B well on the 5 trays.
9. Add three drops of simulated anti-Rh serum in each Rh well on the 5 trays.
 10. Stir each mixture with a different clean toothpick. Use only one toothpick per
well to avoid cross contamination.
11. Observe and record the results below. If you observed agglutination
(clumping) record agglutination in table. If you observed no agglutination
record no agglutination. If the blood agglutinates that means that those
antigens are present.

Data:

Crime Scene #: ____________________

Suspect Anti-A Serum Anti-B Serum Anti-Rh Serum Blood Type

Crime Scene

Mr. Smith

Mr. Jones

Mr. Green

Ms. Brown

Who is the Pioneer Puppet?______________________________________

Questions:
1. What is Mr. Smith’s blood type? What A,B,O antigens are present in his red
blood cells?

2. What is Mr. Green’s blood type? What A,B,O antigens are present in his red
blood cells?

3. What is Mr. Jones blood type? If Mr. Jones needed a transfusion, what blood
type(s) could he safely receive?
4. What is Ms. Brown’s blood type? What blood type(s) could safely receive her
donated blood?

5. Why is it necessary to match the donor’s and recipient’s blood before a

transfusion?

6. How are ABO blood types determined?

7. Could a man with an AB blood type be the father of a child with type O
blood? Show your work for credit.

8. Could a man with an O blood type be the father of a child with type AB
blood? Show your work for credit.

9. What are the possible genetic combinations of a child whose parents’ blood
types are A and B? Show your work for credit.