INTRODUCTION (ALICIA, HARU):

ALICIA

Cellular respiration, the process by which organisms combine oxygen with foodstuff molecules,

diverting the chemical energy in these substances into life-sustaining activities and discarding, as

waste products, carbon dioxide and water. Organisms that do not depend on oxygen degrade

foodstuffs in a process called fermentation. (For longer treatments of various aspects of cellular

respiration, see tricarboxylic acid cycle and metabolism.)

While the pyruvate generated through glycolysis can accumulate to form lactic acid, it can also
be used to generate further molecules of ATP. Mitochondria in the muscle fibers can convert
pyruvate into ATP in the presence of oxygen via the Krebs Cycle, generating an additional 30
molecules of ATP.

Cellular respiration is not as rapid as the above mechanisms; however, it is required for exercise
periods longer than 30 seconds. Cellular respiration is limited by oxygen availability, so lactic
acid can still build up if pyruvate in the Krebs Cycle is insufficient.
Cellular respiration plays a key role in returning the muscles to normal after exercise, converting
the excess pyruvate into ATP and regenerating the stores of ATP, phosphocreatine, and glycogen
in the muscle that are required for more rapid contractions.

https://www.britannica.com/science/cellular-respiration#:~:text=cellular%20respiration%2C
%20the%20process%20by,products%2C%20carbon%20dioxide%20and%20water.

https://med.libretexts.org/Bookshelves/Anatomy_and_Physiology/
Anatomy_and_Physiology_(Boundless)/9%3A_Muscular_System/9.4%3A_Muscle_Metabolism/9.4A
%3A_Muscle_Metabolism

HARU

What is cellular respiration?

Oxygen is used chemically during cellular respiration to convert sugars and carbohydrates into energy.

also known as oxidative metabolism, aerobic metabolism, and aerobic respiration.

The metabolic process known as cellular respiration uses glucose to create adenosine triphosphate (ATP),

an organic chemical the body can use as fuel. A single glucose molecule can generate a net of 30-32 ATP.

What is the purpose of cellular respiration?

Usable ATP energy is produced during cellular respiration and is then used to support a variety of other

bodily functions. For energetically unfavorable reactions that would not take place without an energy

input, ATP is particularly crucial.

This experiment will show how exercise affects the rate of cellular respiration. The rate of breathing,

heartbeat, and carbon dioxide generation will all be measured as three separate markers of cellular

respiration. These indicators will be measured before and after exercising for one and two minutes. The

rate of breathing is expressed in breaths per minute, the rate of heartbeat in beats per minute, and the rate

of carbon dioxide in the amount of time it takes for bromothymol blue to turn color. Carbon dioxide
production can be measured by breathing through a straw into a solution of bromothymol blue (BTB).

BTB is an acid indicator. BTB will change into yellow when you breathe on it.

https://www.osmosis.org/answers/cellular-respiration

HYPOTHESIS (ALEXIS):

If I exercise throughout the day, I will generate ATP. It is an energy carrier, therefore it is produced

during cellular respiration. If I exercise throughout the day, In comparison to when I'm at rest, my body

will produce carbon dioxide more quickly when I'm exercising. Hypothesis: I would produce carbon

dioxide more quickly because in order to use more oxygen, carbon dioxide production would need to

increase as well. As well as how the ATP connects to cellular respiration it is because, the main

byproduct of cellular respiration is ATP, which contains energy that can be utilized by other cellular

activities. Glycolysis produces some ATP. Additionally, NADH generated during glycolysis are utilized

in later stages of cellular respiration to produce even more ATP.

Null hypothesis:

There is no significant difference between not exercising and exercising to the process of cellular

respiration.

Alternative hypothesis:

There is a significant difference between not exercising and exercising to process cellular respiration.

It is significant, if you're not exercising it will decrease the rate of your cellular respiration also if you

don’t exercise it will make your muscle weaker. Weaker muscle takes more oxygen to work so it is

significant for us to exercise daily because it improves our health as well as exercise builds more carbon

dioxide because the rate of cellular respiration is increasing as when the muscle cells created ATP. A

product of cellular respiration is carbon dioxide and every muscle cell contains a small amount of ATP
that can be used right away, lasting only for around 3 seconds. Therefore, a high-energy substance called

creatine phosphate is present in all muscle cells and is swiftly broken down to produce more ATP.

https://www.sciencelearn.org.nz/resources/1920-energy-for-exercise

MATERIALS (DIANE):

•Beaker/Test Tube/Cup

•Bromothymol blue solution (BTB)

•Straw

•Stop watch

PROCEDURE (ANGEL):

This experiment aims to measure carbon dioxide production, breathing rate, and heart rate during

different activities. The procedure can be summarized as follows:

Measuring Carbon Dioxide Production:

1. Measure 20 mL of tap water and transfer it to a small beaker.

2. Add 8 drops of bromothymol blue (BTB) to the water to create a BTB solution.

3. Exhale into the BTB solution using a straw (be cautious not to inhale the solution).

4. Record the time it takes for the blue solution to turn yellow. Repeat this process three times.

5. Calculate the average time for the three trials and record it in Table 1.

Measuring Breathing Rate:

1. Count the number of breaths (inhale + exhale) taken in one minute.

2. Repeat this process two more times.

3. Calculate the average breathing rate from the three trials and record it in Table 2.

Measuring Heart Rate:

1. While measuring the breathing rate, have a partner take your pulse.

2. Count the number of heartbeats in 30 seconds and multiply by 2.

3. Repeat this process two more times.

4. Calculate the average heart rate from the three trials and record it in Table 3.

Increased Muscle Activity (Exercise):

1. Perform jumping jacks for exactly one minute.

2. While exercising, prepare a fresh BTB solution.

3. After one minute of exercise, exhale into the BTB solution and record the time it takes for the

solution to turn yellow in Table 1.

4. Calculate breathing and heart rates as before and record them in Tables 2 and 3.

5. Repeat the exercise for two continuous minutes.

6. Exhale into the BTB solution immediately after exercise and record the time it takes for the

solution to turn yellow in Table 1.

7. Calculate breathing and heart rates and record them in Tables 2 and 3.

8. If time permits, repeat the entire procedure for a lab partner or gather data from two or three

other participants to expand the dataset.

 This experiment provides a comprehensive analysis of carbon dioxide production, breathing rate,

and heart rate during rest and exercise. The data collected can help understand the physiological

changes that occur during different activities.

DATA (PAULEEN, HILARY):

Pauleen:

Table 1. Carbon Dioxide Production (time it takes BTB to change color)

Student 1 Student 2 Student 3 Average

Trial 1 11.04 9.35 9.32 9.90

R
E Trial 2 8.62 6.48 8.58 7.89
S
T Trial 3 7.48 7.53 8.48 7.83
I
N
G Average 9.05 7.79 8.79 8.54

1 minute 5.32 5.29 4.71 5.11

EXERCISE
2 minutes 6.71 3.11 3.82 4.55

Table 2. Breathing rate (breaths/minute)

 Student 1 Student 2 Student 3 Average

Trial 1 14 18 12 14.67
R
E Trial 2 12 18 18 16
S
T Trial 3 14 18 12 14.67
I
N
G Average 13.33 18 14 15.11

1 minute 20 30 30 26.67
EXERCISE
2 minutes 22 30 36 29.33

Table 3. Heart rate (beats/minute)

Student 1 Student 2 Student 3 Average

Trial 1 80 72 54 68.67
R
E Trial 82 72 54 68.67
S
T Trial 3 81 72 48 67
I
N
G Average 81 72 52 68.11

1 minute 110 150 60 106.67

EXERCISE
2 minutes 122 228 80 143.33

Hilary:
Graph 1.

https://www.youtube.com/watch?fbclid=IwAR3j2dGLEKjA_kc9WUS3PyeiiBLCdSxGo4RYq-

RSGq_SM6cd9dYB-YdZfj4&v=nZCd3zgz6Iw&feature=youtu.be

CONCLUSION (SOFIA):

To summarize, cellular respiration is a necessary activity that happens in all living cells

and converts nutrients into energy in the form of ATP. This is required to all living species,

including humans in order to survive and operate.

Exercise may have a considerable influence on the rate of cellular respiration since it

raises the body's demand for energy. During the activity, the body demands more ATP to power

the muscles, thus cellular respiration increases to satisfy this requirement. As a result, this

procedure increases oxygen consumption, which is needed to sustain the electron transport chain

and make additional ATP.

 Regular exercise potentially has long-term impacts on cellular respiration by boosting the

number and efficiency of mitochondria in cells. Mitochondria are the organelles responsible for

cellular respiration, and they may adjust to the increased energy needed by growing more

numerous and efficient.

Overall, exercise plays an important role in maintaining good cellular respiration and

supporting the proper performance of the cells in the body.

QUIZ (JC, PAUL, DUSTIN, JULY, KHAZZY, KURT, MIKO)

JULY

1. How does exercise affect the rate of cellular respiration?

2. How does exercise affect breathing rate, in your opinion?

PAUL

3. What is cellular respiration, and why is it important?

4. What are the three main stages of cellular respiration?

DUSTIN

5. How does cellular respiration process energy?

6. How is cellular respiration connected to ATP?

LIST OF REFERENCES (IRVIN)

Introduction: https://www.britannica.com/science/cellular-respiration#:~:text=cellular

%20respiration%2C%20the%20process%20by,products%2C%20carbon%20dioxide

%20and%20water.

https://med.libretexts.org/Bookshelves/Anatomy_and_Physiology/
Anatomy_and_Physiology_(Boundless)/9%3A_Muscular_System/9.4%3A_Muscle_Metabolism/
9.4A%3A_Muscle_Metabolism

“What is Cellular Respiration?”:

https://www.osmosis.org/answers/cellular-respiration

Hypothesis:

https://www.sciencelearn.org.nz/resources/1920-energy-for-exercise

Data and Graphing:

https://www.youtube.com/watch?fbclid=IwAR3j2dGLEKjA_kc9WUS3PyeiiBLCdSxGo4RYq-

RSGq_SM6cd9dYB-YdZfj4&v=nZCd3zgz6Iw&feature=youtu.be

Pics for the vid:

CELLULAR RESPIRATION: https://www.sciencefacts.net/cellular-respiration.html

EXERCISING: https://health.clevelandclinic.org/how-often-you-should-work-out/

BEAKER:

BROMOTHYMOL BLUE:

STRAW:

STOPWATCH:

SCRIPT (LIANAH, HANNAH):

Hannah:

Introduction (can be shortened if needed.)

Actor: Are you a fitness fanatic? or a freak, perhaps? Do you regularly exercise and go to gyms

to make yourself healthier and your body stronger? You do? Well, I'm not much of an exercise

guy, but you can say I'm trying.

Actor: You are probably wondering right now why I am talking about exercise when our video

is all about cellular respiration. Well, let me tell you.

Actor: When we hear the word respiration, we think of regular breathing and the feeling of your

chest rising and falling as the air fills your lungs. But when we breathe, our cells breathe too - a

process called cellular respiration.

Actor: "Cellular respiration" is the process by which organisms mix oxygen with food

molecules, directing the chemical energy into life-sustaining processes. Oxygen is used to

transform sugars and carbs into energy, while glucose is used to produce adenosine triphosphate

(ATP). This procedure is important for restoring muscle function after exercise.

Actor: To put it simply, exercise causes an increase in the rate of cellular respiration. But let's

take a closer look at this: Does exercise actually affect the rate of cellular respiration? How,

specifically?

Actor: Curious aren't you? Well, I am too. This experiment will measure the rate of cellular

respiratio n by monitoring the rate of breathing, pulse, and carbon dioxide production. The pace

of breathing is measured in breaths per minute, the rate of heartbeat in beats per minute, and the

rate of carbon dioxide in the time it takes for bromothymol blue (BTB) solution to change color.

This can be used to quantify carbon dioxide generation.

Hypothesis:
Actor: If I exercise throughout the day, I will generate ATP. It is an energy carrier, therefore it is

produced during cellular respiration. If I exercise throughout the day, In comparison to when I'm

at rest, my body will produce carbon dioxide more quickly when I'm exercising.

Null hypothesis:

There is no significant difference between not exercising and exercising to the process of cellular

respiration.

Alternative hypothesis:

There is a significant difference between not exercising and exercising to process cellular

respiration.

Actor: In significant, exercise is helpful to our health because it accelerates cellular respiration

and produces more carbon dioxide. Muscle cells contain a modest quantity of ATP that is

available for immediate usage and lasts only 3 seconds. Creatine phosphate is found in all muscle

cells and is rapidly degraded to generate additional ATP. Daily exercise enhances our health

while emitting more CO2.

Materials:

Actor: Now that we've defined cellular respiration and the assumption of our experiment, let's

get started by determining the materials needed.

Actor: The first material is a Beaker in which we will pour the water. If you don't have a beaker,

you may use test tubes or a cup instead. A pH indicator, Bromothymol blue solution, is also

required. You will also need a straw and a stopwatch for this experiment.

Actor: As soon as we have all of our materials ready, we may begin this experiment's procedure.

Procedure:
Actor: We will carry out four different procedures as part of our experiment. During different

activities, the experiment will monitor carbon dioxide production, breathing rate, and heart rate.

Actor: This experiment will provide a comprehensive analysis of carbon dioxide production,

breathing rate, and heart rate during rest and exercise. The data collected can help understand the

physiological changes that occur during different activities.

Actor: Following is a synopsis of the process:

Measuring Carbon Dioxide Production:

1. Measure 20 mL of tap water and transfer it to a small beaker.

2. Add 8 drops of bromothymol blue (BTB) to the water to create a BTB solution.

3. Exhale into the BTB solution using a straw (be cautious not to inhale the solution).

4. Record the time it takes for the blue solution to turn yellow. Repeat this process three times.

5. Calculate the average time for the three trials and record it in Table 1.

Measuring Breathing Rate:

1. Count the number of breaths (inhale + exhale) taken in one minute.

2. Repeat this process two more times.

3. Calculate the average breathing rate from the three trials and record it in Table 2.

Measuring Heart Rate:

1. While measuring the breathing rate, have a partner take your pulse.

2. Count the number of heartbeats in 30 seconds and multiply by 2.

3. Repeat this process two more times.

4. Calculate the average heart rate from the three trials and record it in Table 3.

Increased Muscle Activity (Exercise):

1. Perform jumping jacks for exactly one minute.

2. While exercising, prepare a fresh BTB solution.

3. After one minute of exercise, exhale into the BTB solution and record the time it takes for the

solution to turn yellow in Table 1.

4. Calculate breathing and heart rates as before and record them in Tables 2 and 3.

5. Repeat the exercise for two continuous minutes.

6. Exhale into the BTB solution immediately after exercise and record the time it takes for the

solution to turn yellow in Table 1.

7. Calculate breathing and heart rates and record them in Tables 2 and 3.

8. If time permits, repeat the entire procedure for a lab partner or gather data from two or three

other participants to expand the dataset.

Lianah:

Data:

Actor: Now that the experimentation is done, I will now tell you the results.

Actor: For table 1 of the carbon dioxide production or the time BTB takes to change color while

resting, Student 1’s trial averaged 9.05 seconds; Student 2’s trial averaged 7.79 seconds; and

lastly, Student 3’s trial averaged 8.79 seconds. In total, the average carbon dioxide production, or

the time BTB takes to change color while resting, is 8.54 seconds. Now let’s get into the results

of the carbon dioxide production or the time BTB takes to change color while exercising. With 1

minute of exercise, Student 1’s result is 5.32 seconds; Student 2’s result is 5.29 seconds; and
Student 3’s result is 4.71 seconds, totaling an average of 5.11 seconds. For 2 minutes of exercise,

Student 1’s result is 6.71; Student 2’s result is 3.11; and Student 3’s result is 3.82 seconds, which

resulted in an average of 4.55 seconds.

Actor: For table 2, the average breathing rate at rest for Student 1 is 13.33 seconds, Student 2 is

18 seconds, and Student 3 is 14 seconds, which totals to an average of 15.11 breaths per minute.

With 1 minute of exercise, Student 1’s breathing rate has increased to 20 breaths per minute, and

both Students 2 and 3 have now reached 30 breaths per minute, resulting in an average of 26.67

breaths. With 2 minutes of exercise, Student 1’s breathing rate increased to 22 breaths per

minute, while Student 2’s breathing rate remained the same, and Student 3’s breathing rate

increased from 30 to 36 breaths per minute, which totaled to an average of 29.33 breaths per

minute.

Actor: For table 3, the average heart rate at rest of Student 1 is 81 beats per minute, Student 2 is

72 beats per minute, and Student 3 is 52 beats per minute, resulting in an average of 68.11 beats

per minute. With 1 minute of exercise, Student 1’s heart rate has increased to 110 beats per

minute, 150 beats per minute for Student 2, and 60 beats per minute for Student 3, totaling an

average of 106.67 beats per minute. With 2 minutes of exercise, Student 1’s heart rate increased

to 122 beats per minute, Student 2’s heart rate increased to 228 beats per minute, and Student 3’s

heart rate increased to 80 beats per minute, which totals to an average of 143.33 beats per

minute.

Actor: According to Graph 1, it is shown that the time for BTB to change its colors has

decreased because of exercising from 8.54 seconds to 4.55 seconds, while the breaths per minute

(15.11, 26.67, and 29.33) and beats per minute (68.11, 106.67, and 143.33) have increased.
Actor: All in all, all the data from the table and the graph shows that exercise has an effect on

cellular respiration.

Conclusion:

Actor: To summarize, cellular respiration is a necessary activity that happens in all living cells

and converts nutrients into energy in the form of ATP. This is required to all living species,

including humans in order to survive and operate. Exercise has a considerable influence on the

rate of cellular respiration since it raises the body's demand for energy. During the activity, the

body demands more ATP to power the muscles, thus cellular respiration increases to satisfy this

requirement. As a result, this procedure increases oxygen consumption, which is needed to

sustain the electron transport chain and make additional ATP. Regular exercise potentially has

long-term impacts on cellular respiration by boosting the number and efficiency of mitochondria

in cells. Mitochondria are the organelles responsible for cellular respiration, and they may adjust

to the increased energy needed by growing more numerous and efficient. Overall, exercise plays

an important role in maintaining good cellular respiration and supporting the proper performance

of the cells in the body based on the experimentation that we’ve conducted.

Quiz:

Actor: Now that you’ve learned about how exercise can cause “cell respiration,” I would like to

test your knowledge to know that you’ve truly understood what it’s all about.

Actor: Once again, I’m Francis Calos, and I’m your host for today. “Welcome to Bioloquiz” A

quiz that tests your knowledge about biology, and today it’s all about cell respiration.

Actor: Question no. 1: What is cellular respiration, and why is it important?

(TIMER STARTS NOW! (5, 4, 3, 2, 1, 0))

Actor: Yes, that’s right, "cellular respiration" is the process by which organisms mix oxygen with food

molecules, directing the chemical energy into life-sustaining processes. Oxygen is used to transform

sugars and carbs into energy, while glucose is used to produce adenosine triphosphate (ATP), which

makes this procedure important for restoring muscle function after exercise.

Actor: Question no. 2: What are the three main stages of cellular respiration?

(TIMER STARTS NOW! 5, 4, 3, 2, 1, 0)

Actor: Yup, that’s it. The three main stages of cellular respiration (aerobic) would include

glycolysis, the Krebs cycle, and the electron transport chain. Glycolysis breaks down glucose

into two pyretic acid molecules in the cytoplasm, releasing ATP and hydrogen. The Krebs Cycle

takes citric acid, which is a derivative of pyruvic acid, and converts it through four cycles into

hydrogen, carbon dioxide, and water in the mitochondrial matrix. The electron transport chain

takes the electron-carrying hydrogens and releases the energy as ATP and water in the

mitochondrial membranes.

Actor: Question no. 3: How is cellular respiration connected to ATP?

(TIMER STARTS NOW! 5, 4, 3, 2, 1, 0)

Actor: Correct! Cellular respiration is a metabolic pathway that uses glucose to produce adenosine

triphosphate (ATP), an organic compound the body can use for energy.

Actor: Question no. 4: How does cellular respiration process energy?

(TIMER STARTS NOW! 5, 4, 3, 2, 1, 0)

Actor: Good job! Glycolysis breaks down glucose into two pyretic acid molecules in the

cytoplasm, releasing ATP and hydrogen. The Krebs Cycle takes citric acid, which is a derivative

of pyruvic acid, and converts it through four cycles into hydrogen, carbon dioxide, and water in

the mitochondrial matrix. The electron transport chain takes the electron-carrying hydrogens and

releases the energy as ATP and water in the mitochondrial membranes.

Actor: Question no. 5: How does exercise affect the rate of cellular respiration?

(TIMER STARTS NOW! 5, 4, 3, 2, 1, 0)

Actor: Nice, that’s right! Exercise increases the rate of cellular respiration. This occurs because

of the need for greater amounts of ATP in the muscle tissue. As we contract our muscles, more

ATP is needed to form and release the actin and myosin filaments during a contraction.

Actor: Question no. 6: How does exercise affect breathing rate, in your opinion?

(TIMER STARTS NOW! 5, 4, 3, 2, 1, 0)

Actor: Way to go! Your breathing rate increases, so you can get more oxygen to make more energy.

Actor: Congratulations!! I hope that you’ve learned something from me, and that’s it for "Bioloquiz."

Good bye!!

Actor: That’s it, and thank you for listening to our topic, which is cell respiration! I hope you have a

wonderful day!