Experiment Number: 03

Date: September,01, 2023

Name: k.nemalnath reddy

Registration number: 2023003651

Determination of Zinc by EDTA

Method
Objective:
To learn about,

 To understand the behavior of EDTA as chelating agent .

 To know the method of complexometric titration and estimation of metal in materials.

 To know the stability of complexes at particular pH’s.

 Complexometric volumetric titrations with EDTA (ethylenediaminetetraacetic acid) will be

performed. The comprehension and skills learned will be transferable to other laboratory
and workplace situations.

• A primary-standard zinc ion solution will be prepared from primary-standard zinc metal.

• A supplied EDTA solution will be standardized using the primary-standard zinc ion solution.

• The secondary standard EDTA solution will be employed to determine the zinc content of a dietary
supplement.

Introduction:
Determination of the Zinc (Zn+2) by titrating against a standard EDTA solution using Eriochrome
Black-T (EBT) as indicator.

Zinc present in a given sample can be determined by using the complexometric method, in which the
disodium salt of EDTA is employed (soluble in water) and it can be represented as follows.

(EDTA – Ethylene Diamine Tetra Acetic acid)

 EDTA forms complexes with zinc when the pH is in the range of around 9.5 to 10.5 and to maintain
the pH, a basic buffer solution is used (NH4OH + NH4Cl buffer serves pH 9.5 to 10.5). The complexes
of Zinc with EDTA are colorless, therefore it is necessary to use indicator to locate the end point. In
this titration Eriochrome black – T is used as indicator, which forms an unstable wine red colored
complex with Zinc, calcium and magnesium.. So the color change at the end point is wine red to blue.
(M =Metal ions, Zn+2 )

The di-sodium salt of EDTA solution can be standardized by using standard zinc sulphate solution in
presence of ammonia – ammonium chloride buffer (pH 10) using Eriochrome Black – T as indicator.

Materials and Equipment:

Chemicals:

Ethylenediaminetetraacetic acid (EDTA), ammonium hydroxide, ammonium chloride, Eriochrome Black T

(EBT), zinc sulphate.

Apparatus: Standard volumetric flask, pipette, burette, conical flask, beakers, funnel and glass rod.

Procedure:
 Standardization of EDTA (~0.01M) Rinse and fill the burette with EDTA solution. Pipette out
10 mL of 0.01M of ZnSO4 solution and add 10 mL of the buffer of pH 10.
 Add 2-3 drops of EBT indicator solution to the flask.
 Titrate the solution from the flask against EDTA solution until the red colour of the solution
changes to blue.
 Repeat the titration to get constant readings.
 Estimation of Zn2+ ions. Rinse and fill the burette with EDTA solution.
  Pipette out 10 mL of Zn2+ ion solution in a conical flask and add 10 mL of the buffer of pH
10.
 Add 2-3 drops of EBT indicator solution to the flask.
 Titrate the solution from the flask against EDTA solution until the red colour of the solution
changes to blue.
 Repeat the titration to get constant readings.

Data and Observations:

The final reading of the burette is noted. Titrations are repeated until 3 or 4 concurrent readings are
obtained. The results are tabulated in Table II.

Table II:

Titration of standard EDTA solution with Unknown zinc solution Molarity of standard solution of
EDTA = Indicator: Eriochrome black-T.

Color change at the end point: Wine red to blue

Sl. No. Volume of Burette Burette Volume of

unknown readings readings zinc solution
EDTA Initial (ml) Final (ml) consumed in
solution ml (V1)
taken in ml
(V2) M
01 10ML 0 8.1 8.1
02 10ML 8.1 15.7 7.6
03 10ML 15.7 23.7 8
AVERAGE:

8.1+7.6+8/3=7.9ML

Calculations and Results:

By the law of equivalence,

M 1V1=M2V2

Molarity of EDTA solution M2 =0.01M

Volume of EDTA solutionV2=10ml

Molarity of Zinc solution M1=?

Volume of Zinc solutionV1=7.9ml

 M1 =M2*V2/V1
 =0.01*10/7.9

=0.0126M

Report:

The Molarity of Zinc solution, M4 =0.0126M

Equivalent weight of Zinc = atomic weight / 1 = 65.38 / 1 = 65.38 M

Amount of zinc present in 1000 ml of solution = molarity of zinc solution (M1)  equivalent weight of
zinc =0.0126*65.38=0.827 g.

Amount of copper present in the given 100 ml of zinc solution = amount present in 1 liter of the solution
/ 10 =0.827/10 = 0.0827g.

Result:

The amount of zinc present in given unknown sample = 0.0827g.

Discussion: (if any)

The concentration of the Zn solution was determined to be approximately 14 mol/L through the titration
with EDTA. This result is within an acceptable range of the expected concentration.

Conclusion:
The objective of the experiment was successfully achieved by accurately determining the concentration
of the Zn solution. The calculated concentration was 0.0827g, indicating that the experiment was carried
out effectively.

References:
Experiments in Applied Chemistry, S. Rattan, S. K Kataria and Sons.

Vogel’s textbook of quantitative chemical analysis, J. Mendham, R. C. Denney, J. D. Barns, M.

Thomas, B. Sivasankar, Pearson India Education, 6th Edition.

The student remarks on experiment:

Sources of Error:

1. regular water instead of distilled water.

2. No Care is taken while preparing cement solution .
3. There may be any leakage from burette.
4. Before using any solution, not shaking it properly.

Precautions:
  The fumes from the NH3/NH4Cl buffer at pH 10 are toxic, corrosive, and irritating.
 Wash hands thoroughly before leaving the lab.
 Eriochrome black-T is a dye stuff and avoid direct contact since it cause eye irritation and
skin irritation.
 Don’t add excess amount indicator to avoid error.
REMARKS:

There are no remarks for the given activity.