5/9/23, 10:56 AM Fermentation process of Penicillin

Home  Pharmaceutical Biotechnology  Fermentation process of

Penicillin

Fermentation process of
Penicillin
 Prem  January 29, 2023

Fermentation
process of Penicillin
Contents
• Introduction
Telegram
• Mechanism of action

• Adverse effects Channel Pharman

Pharmano
• Production - Fermentation
provide N

Objectives t.me

At the end of the lecture, student will be able to

• Discuss the mechanism of action and adverse effects of

Penicillin

• Explain the fermentation process of Penicillin

Pharmacology
Penicillin Anti-Diabetic Drugs,
• First antibiotic - scottish scientist - Alexander Fleming - Insulin and Oral
1928 hypoglycemic
drugs  October 13, 2022
• PCN – isolated from fungus – Penicillium notatum

• ß-lactum antibiotics Sulfonamides and

Cotrimoxazole  Febr
• Active against – Gram +ve bacteria (Staphylococci and
uary 05, 2022
Streptococci) not against gram –ve except at high dose
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Mechanism of Action
OPC
• Interferes with the cell wall synthesis of bacteria Poisoning  February

• Inhibit the formation – peptidoglycan cross links in 04, 2022

bacterial cell wall – binding of ß-lactum ring of penicillin to

enzyme DD-transpeptidase Penicillins  February

04, 2022
• DD-transpeptidase – cannot catalyze – formation of cross
links – imbalance in cell wall production – degrades – rapid
Anti-ulcer
cell death
Drugs  February 04,
Adverse effects 2022

• 10 % people – allergic reactions

Macrolides  February

• ≥ 1% of people – diarrhoea, hypersensivity, nausea, rashes, 04, 2022

neurotoxicity and urticaria

• Pain and inflammation at the site of injection Emetics  February 04,

2022
• 0.1- 1% - fever, vomiting, erythema, dermatitis, seizures

Route of Administration: Chronopharmacolog

y  February 04, 2022

Also read : Vaccines

• Oral

• IV
Pharmacognos
Use:
• Otitis Media Tannins, Physical and
Chemical Nature,
• Meningitis Classification  Decem
• Sore Throat ber 27, 2022

• Pneumonia & Respiratory Infection

Chemical tests,
• Septicemia isolation and
estimation of
• Peritonitis
Tannins  December 27,

• Gonorrhea 2022

• UTI
Cardiovascular,
Known Issues antimicrobial, anti-
inflammatory and
• Resistance (B-lactmase & other mechanisms) antibiotic agents of
Allergic rxns marine

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• Cross-hypersensitivity (1-3% w/cephalosporins) origin  December 19,

2022

General methods of
isolation and
purification;
anticancer, agents of
marine
origin  December 19,

2022

Present status and

Classification of
marine
drugs  December 19,
2022

Isolation and Fusion

of
Protoplast  December

18, 2022

Classification and
Isolation of
Resins  December 18,
2022

Study of Bromelain,
Urokinase,
Streptokinase  Dece

mber 11, 2022

Popular
Production Posts
Pathophysiology - B.
Pharma 2nd Semester
• Stationary mat culture notes pdf

• Medium – sterilized – shallow layers – bottles, flasks, trays

PHARMACOLOGY &
or pans – glass or metal TOXICOLOGY D. Pharm
2nd year PDF Notes
• Wet or dry spores – added – medium or blown on its
surface
D. Pharmacy Notes | D.
• Incubated – 24 – 28˚C for six or seven days Pharma Notes | Study

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• Equipment and labor required material Diploma in

Pharmacy pdf
Also read : Genetic organization of
Eukaryotes and Prokaryotes Drug Store and Business
Management - D.
Pharma 2nd Year
• Harvest – pooling all the broths – but contaminated
Notes
(penicillinase enzyme) – separated and discarded

Pharmaceutical
• Problems –Low yield
Jurisprudence - PDF
– Oxygen penetration is low notes & Important
Question Answer
– Portion of mycelium submerged - medium -
deficient in O2 Industrial Pharmacy
(Theory) - B. Pharma 5th
– Aerial hyphae – poor access to nutrients Semester PDF Notes

• If not – penicillinase – will destroy all the pooled penicillin

B. Pharma Notes | B.
• Deep tank aerated fermentation – World War II Pharma Notes | Study
material Bachelor of
Fermentation Process Pharmacy pdf

• Several different fungi are used Hospital Clinical

Pharmacy - D. Pharma
• Principal organism used – commercial production – 2nd Year Notes
Mutated strains – Penicillium chrysogenum
PHARMACEUTICAL
Media Composition (Jackson, 1958) CHEMISTRY II - D.
Pharm 2nd Year Short
Trade secrets
Notes
• Cornsteep liquor solids – 3.5 %
Stroke - B. Pharma 2nd
• Lactose – 3.5 % Semester
Pathophysiology notes
• Glucose – 1 % pdf

• Calcium carbonate – 1 %

• Potassium dihydrogen phosphate – 0.4 %

• Edible oil – 0.25 %

Categories
• Penicillin precursor
 Analysis 32
Production media: (Sylvester and
Coghill, 1954)  API 3

• Corn steep liquor solids – 30 gm  Biochemistry And

Clinical Pathology 52

Also read : Pharmaceutical Biotechnology -  Biopharmaceutics &

B. Pharma 6th Semester Notes Pdf Pharmacokinetics 13

 Calibration 37
• Lactose – 30 gm

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• Glucose – 5 gm  Communication Skill 21

• Sodium nitrate – 3 gm  Drug Store And

Business Management
• Magnesium sulphate – 0.25 gm 4
 Environmental
• Zinc sulphate – 0.044 gm Sciences 32

• Calcium carbonate - 3 gm  Exam Guide 21

• Penicillin precursor  Health Education And

Community Pharmacy

Step 1: Stock culture Maintenance  Herbal Drug

2

Technology 19
• High yielding strains of Penicillium chrysogenum
 Hospital & Clinical
• Agar media – minimum vegetative growth and rapid Pharmacy 5
sporulation of entire culture
 Human Anatomy And
• Media – inoculated – spores Physiology 55

 Industrial Pharmacy 30
This primary stock can maintained as
 Inorganic Chemistry 20
• Well-sporulated agar slants – frozen
 Instrumental Methods
• Soil stocks are prepared and stored at 2-4˚C Of Analysis 25

• Lyophilized  Jurisprudence 32

Step 2: Inoculum preparation  Lab Manual 4

 Medicinal Chemistry 65
• Spores - heavily sporulated working stock – suspended in
water or dil solution of non-toxic wetting agent, 1:10,000 SLS  NDDS 25

• Added to bottles or flasks – wheat bran + nutrient  Pathophysiology 63

(Inoculum media – similar to production media – except
lactose and precursor not added)  Pharmaceutical
Biotechnology 11

• Incubated - 24 ˚C for 5-7 days – to provide heavy  PHARMACEUTICAL

CHEMISTRY 4
sporulation
 Pharmaceutical
• Spores – used – inoculate inoculum tanks Microbiology 45

• Inoculum tanks – incubated – 24-48 hrs – agitation and  Pharmaceutical

aeration – production tank Organic Chemistry 70

 Pharmaceutics 13
Also read : Enzymes - Definition, 9
properties, classification, applications &  Pharmacognosy And
Isolation Phytochemistry 77

 Pharmacology 82
• Contamination – inoculum tanks – microscopical
 Pharmacy Practice 28
observations and sub culturing in broth medium

Production  Physical Pharmacy 7

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• Penicillin fermentation – 25-26  PPT 42

• 3-5 days  Practice School 7

• Exact duration depends on  Project 20

– Fungal strain  QUALITY ASSURANCE

17
– Type of production media  SOP's 30
4
– Aeration and agitation conditions

• Periodic samples – penicillin yield and contamination

check – sensitive to penicillinase producing organisms
Total
Pageviews
Course of Penicillin Production 5234250

Tags
Analysis API

Biochemistry and

Clinical Pathology &

Biopharmaceutics

Pharmacokinetics
Calibration

communication skill

Drug Store and

Harvest and Recovery Business
Environmental
Management
Sciences
Exam Guide

Health Education and

Community
herbal drug
Pharmacy
technology
Hospital & Clinical

Pharmacy
Human Anatomy and

Physiology
Industrial pharmacy

Inorganic Chemistry

Instrumental Methods

of Analysis
Jurisprudence
Summary Lab Manual
• Principal organism used – commercial production –
Medicinal Chemistry
Mutated strains – Penicillium chrysogenum
NDDS
• Penicillin fermentation – 25-26
Pathophysiology

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• 3-5 days Pharmaceutical

Biotechnology
PHARMACEUTICAL
• Exact duration depends on
CHEMISTRY
Pharmaceutical
– Fungal strain
Microbiology
Pharmaceutical
– Type of production media
Organic Chemistry
Pharmaceutics
– Aeration and agitation conditions
Pharmacognosy and
• Periodic samples – penicillin yield and contamination
Phytochemistry
Pharmacology
check – sensitive to penicillinase producing organisms
Pharmacy Practice

Physical Pharmacy

PPT

Practice School
Tags: Pharmaceutical Biotechnology

Project

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SOP's
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Fermentor Fermentation process of
Vitamin B12

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