Exportlist

TY - CONF
AB - The proceedings contain 2 papers. The topics discussed include: carbonaceous

nanoparticles and their interactions with biological cells; dose-dependent
intracellular reactive oxygen and nitrogen species production from particulate
matter exposure: results from ambient samples and chamber experiments; biofuel and
reference diesel particles: differences in inflammatory and oxidative effects; real
life PM emissions from traffic and human exposure implications; disinfection of
water using silver and copper nanoparticle impregnated activated carbon; amorphous
silicon dioxide nanoparticle interactions with pulmonary epithelial cells with and
without a pre-existing protein corona; physiochemical properties of nanoparticles
determine their in vitro cytotoxicity; potential impact of sublethal levels of
nanomaterials on interactive behavior of environmental bacteria; effect of size and
charge of metal ions on hydrogen peroxide stability in silica hydrogels; UV-
assisted synthesis of carbon nanotube-TiO2 nanocomposites for enhanced
photocatalytic air purification; effect of thermal treatment on the characteristics
of PES/PVA nanocomposite membranes modified with TiO2 nanoparticles: a comparitive
study between 1-step and 2-step thermal treatment; and eco-friendly dyeing of
electrospun cellulose nanofibers with reactive dye using ultrasonic energy.
AN - rayyan-553780015
DA - 2016
N1 - Export Date: 28 August 2023; Cited By: 0
ST - Environmental Aspects, Applications and Implications of Nanomaterials and
Nanotechnology 2016 - Topical Conference at the 2016 AIChE Annual Meeting
T2 - Environmental Aspects, Applications and Implications of Nanomaterials and
TI - Environmental Aspects, Applications and Implications of Nanomaterials and
UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-
85019046999&partnerID=40&md5=db7be0158733a68aa7d6cb78ce0afbb0
ID - 8056
ER -
TY - CASE
A4 - Ellender, G.
A2 - Ham, K. N.
AB - The retention of an implanted silver wire in human tissue 40 years after
surgical fixation of the frontal bone was studied post mortem by histology,
transmission electron microscopy, and energy-dispersive x-ray analysis. Corrosion
products of the wire were associated with a chronic inflammatory response and were
bound to certain connective tissue elements; they were deposited as discrete
particles, comprising silver in association with sulphur, on collagen fibrils and
vascular basement membranes. Bone structure appeared normal except close to the
wire, where it was replaced by a loose connective tissue in which collagen bundles
were disorganized.
AN - rayyan-553782251
J2 - J Oral Implantol
KW - Aged
*Biocompatible Materials
*Dental Implants
Follow-Up Studies
Humans
Male
*Silver
LA - eng
NV - United States
PY - 1989
SP - 47-51
ST - The Journal of oral implantology
TI - Silver wire implant of 40 years' duration: influence on local tissues
UR - https://pubmed.ncbi.nlm.nih.gov/2634778/
VL - 15
ID - 10161
ER -
TY - CASE
A4 - Büchner, S. A.
AB - We report on a 34-year-old patient suffering from erythema chronicum migrans,
who developed clinically and histologically typical morphea, which was confined to
the area previously involved by the erythema migrans. The patient's serum antibody
level against borrelia burgdorferi spirochetes was significantly elevated. By means
of the silver impregnation technique, we were able to identify spirochetal
organisms both in the lower dermis and within the septa of the subcutaneous fatty
tissue. In frozen sections, spirochetes were demonstrated by the immunoperoxidase
method using specific anti-spirochetal antibodies. For a better assessment of the
composition of the inflammatory infiltrate in morphea, we applied a panel of
monoclonal antibodies to a sensitive two-stage immunoperoxidase technique. Helper-
inducer T-cells and a large number of suppressor-cytotoxic T-cells were observed
both in the perivascular infiltrate and between collagen fibers in close proximity
to the HLA-DR reactive fibroblasts. A large number of mast cells were seen in the
dermal infiltrate. The detection of spirochetal organisms in histological sections
as well as the demonstration of closely associated helper-inducer T-cells,
macrophages, and activated fibroblasts in the dermis strongly suggest that a cell-
mediated immune response against borrelia may be the dominant pathogenetic event in
this variant of scleroderma. Activated T-lymphocytes and various factors secreted
either by activated lymphocytes or mast cells may cause proliferation of
fibroblasts, which can lead to increased collagen synthesis and dermal fibrosis.
AN - rayyan-553782376
J2 - Z Hautkr
KW - Adult
Animals
Bites and Stings/*complications
Borrelia burgdorferi Group/immunology/ultrastructure
Erythema Chronicum Migrans/*complications/pathology
Humans
Immunoenzyme Techniques
Immunoglobulin G/analysis
Male
Scleroderma, Localized/*pathology
Skin/pathology
*Ticks
Scleroderma, Localized
Ticks
Skin
LA - ger
N1 - Dermatologische Universitätsklinik Basel.
NV - Germany
PY - 1989
SP - 661-4, 667-9
ST - Zeitschrift fur Hautkrankheiten
TI - [Morphea--a tick transmitted borreliosis of the skin? A contribution to the
pathogenesis of circumscript scleroderma]
VL - 64
Y2 - 8 y3 - 15
ID - 10283
ER -
TY - CASE
A4 - Kaufmann, T.
A2 - Bloch, C.
A2 - Schmidt, W.
A2 - Jonas, L.
AB - A 55-year-old woman, suffered from severe pain in her mandibular jaw for
several years. A metallic artifact of about 2(3) mm was detected by a panorama
radiography in an edentulous region with a surrounding inflammation in close
contact to the canal of the mandibular nerve. Inflammated tissue with the central
metallic inclusion was removed from the bone under local anesthesia and operation.
Postoperatively, pain and missensitivity disappeared within 1 week. Although the
patient had no macroscopically visible so-called amalgam tattoo, the metallic cube
was identified as amalgam by the detection of mercury, silver, tin, copper, and
zinc using energy dispersive X-ray analysis (EDX) in a scanning electron microscope
(SEM). Nevertheless, brown to black pigments in the connective tissue matrix and
inside histiocytes, fibroblasts, and multinucleated foreign giant cells of the
surrounding inflammatory tissue were observed by light and electron microscopy.
However, the elemental analysis by EDX in SEM or by electron energy loss
spectroscopy in transmission electron microscope detected only silver, tin, and
sulfur but no mercury in these precipitates and in the residual bodies of
phagocytes. The presented case demonstrates a seldom complication of amalgam
deposition in the tissue. The authors assume that the chronic pain results from a
forgotten amalgam filling inside an alveole after extraction of a molar tooth,
causing a chronic inflammation by resolving mercury and other toxic elements out of
the metallic artifact.
AN - rayyan-553782354
DO - 10.1080/01913120500267554
J2 - Ultrastruct Pathol
KW - *Dental Amalgam/analysis
Facial Pain/diagnostic imaging/*etiology
Female
Foreign-Body Reaction/*etiology
Humans
Mandible/diagnostic imaging/*pathology
Middle Aged
Radiography
Stomatitis/*etiology
Time Factors
Tooth Extraction
Inflammation
Molar, Third
LA - eng
N1 - Department of Maxillofacial Surgery, Medical Faculty, University of Rostock,
Rostock, Germany.
NV - England
PY - 2005
SP - 405-13
ST - Ultrastructural pathology
TI - Chronic inflammation and pain inside the mandibular jaw and a 10-year
forgotten amalgam filling in an alveolar cavity of an extracted molar tooth
VL - 29
Y2 - 9
ID - 10262
ER -
TY - CASE
A4 - Rahman, W.
A2 - Conley, A.
A2 - Silver, K. D.
AB - Checkpoint inhibitor immunotherapy has revolutionised cancer treatment since
its inception. During an inflammatory response, activated cytotoxic T cells
expressing programmed cell death protein 1 (PD-1) interact with programmed cell
death-ligand 1 (PD-L1) on peripheral tissues to thwart an autoimmune reaction.
Cancer cells upregulate PD-L1 expression to evade the immune system and are
vulnerable to attack in the presence of PD-1 or PD-L1 checkpoint inhibitors.
However, blockade of this pathway also contributes to the unintended side effect of
autoimmune endocrinopathies. Atezolizumab, a checkpoint inhibitor against PD-L1, is
associated with the rare complication of type 1 diabetes. We present a case of
glutamic acid decarboxylase antibody-positive type 1 diabetes developing in a
patient with a long-standing history of well-controlled type 2 diabetes following
treatment with atezolizumab for metastatic renal cell carcinoma.
AN - rayyan-553782397
DO - 10.1136/bcr-2019-233842
J2 - BMJ Case Rep
KW - Antibodies, Monoclonal, Humanized/*adverse effects/therapeutic use
Antineoplastic Agents/*adverse effects/therapeutic use
B7-H1 Antigen/metabolism
Carcinoma, Renal Cell/*drug therapy/secondary
Diabetes Mellitus, Type 1/*chemically induced
Diabetes Mellitus, Type 2/complications
Glutamate Decarboxylase/metabolism
Humans
Immune Checkpoint Inhibitors/adverse effects/therapeutic use
Immunotherapy/adverse effects
Kidney Neoplasms/*drug therapy/pathology
Liver Neoplasms/drug therapy/secondary
Male
Middle Aged
Programmed Cell Death 1 Receptor/metabolism
Treatment Outcome
Carcinoma, Renal Cell
LA - eng
N1 - Department of Medicine, University of Maryland Baltimore, Baltimore,
Maryland, USA.; Department of Medicine, University of Maryland Baltimore,
Baltimore, Maryland, USA.; Department of Medicine, University of Maryland
Baltimore, Baltimore, Maryland, USA ksilver@som.umaryland.edu.
NV - England
PY - 2020
ST - BMJ case reports
TI - Atezolizumab-induced type 1 diabetes mellitus in a patient with metastatic
renal cell carcinoma
VL - 13
Y2 - 7 y3 - 2
ID - 10304
ER -
TY - JOUR
AB - Declaration of Competing Interest statements were not included in the
published version of the following articles that appeared in previous issues of
Biocatalysis and Agricultural Biotechnology. The appropriate Declaration/Competing
Interest statements, provided by the Authors, are included below. 1.
“Biodegradation of triphenylmethane dye malachite green by a newly isolated fungus
strain” [Biocatalysis and Agricultural Biotechnology, 2019; 17C; 672–679]
https://10.1016/j.bcab.2019.01.030 Declaration of competing interest: The authors
declare that they have no known competing financial interests or personal
relationships that could have appeared to influence the work reported in this
paper.2. “Microbial lipases: An overview of screening, production and purification”
[Biocatalysis and Agricultural Biotechnology, 2019; 22C: 101368] DOI-
https://10.1016/j.bcab.2019.101368 Declaration of competing interest: The authors
paper.3. “Addition of electron shuttling compounds and different pH conditions for
hydrogen production by a heat-treated sludge” [Biocatalysis and Agricultural
Biotechnology, 2020; 23C: 101507] DOI – https://10.1016/j.bcab.2020.101507
Declaration of competing interest: The authors declare that they have no known
competing financial interests or personal relationships that could have appeared to
influence the work reported in this paper.4. “Enzymatic esterification for the
synthesis of butyl stearate and ethyl stearate” [Biocatalysis and Agricultural
Biotechnology, 2018; 16C: 373–377] DOI – https://10.1016/j.bcab.2018.09.008
influence the work reported in this paper.5. “Enzyme-catalyzed production of
emollient cetostearyl stearate using different immobilized commercial lipases under
vacuum system” [Biocatalysis and Agricultural Biotechnology, 2018; 15C: 229–234]
DOI – https://10.1016/j.bcab.2018.06.012 Declaration of competing interest: The
authors declare that they have no known competing financial interests or personal
paper.6. “Production, Characterization and Emulsifying property of
exopolysaccharide produced by marine isolate of Pseudomonas fluorescens”
[Biocatalysis and Agricultural Biotechnology,2018; 16C: 320–325] DOI –
https://10.1016/j.bcab.2018.08.023 Declaration of competing interest: The authors
paper.7. “Isolation, screening and characterization of plant growth promoting
rhizobacteria from rhizospheric soils of selected pulses” [Biocatalysis and
Agricultural Biotechnology, 2020; 27C: 101685] DOI –
paper.8. “Anti-inflammatory activity of a serine protease produced from bacillus
pumilus SG2” [Biocatalysis and Agricultural Biotechnology, 2019; 17C: 538–544] DOI
– https://10.1016/j.bcab.2019.01.015 Declaration of competing interest: The authors
paper.9. “Highly efficient fungal pectinase and laccase producers among isolates
from flax retting liquor” [Biocatalysis and Agricultural Biotechnology, 2020; 25C:
101570] https://10.1016/j.bcab.2020.101570 Declaration of competing interest: The
paper.10. “Spectral characterization of bioactive compounds from microalgae: N.
Oculata and C. Vulgaris” [Biocatalysis and Agricultural Biotechnology, 2019; 19C:
101094] DOI – https://10.1016/j.bcab.2019.101094Declaration of competing interest:
The authors declare that they have no known competing financial interests or
personal relationships that could have appeared to influence the work reported in
this paper.11. “In silico molecular docking of astaxanthin and sorafenib with
different apoptotic proteins involved in hepatocelllar carcinoma” [Biocatalysis and
Agricultural Biotechnology, 2019; 19C: 101076] https://10.1016/j.bcab.2019.101076
influence the work reported in this paper.12. “Studies on Estrone Biodegradation
Potential of Cyanobacterial Species” [Biocatalysis and Agricultural Biotechnology,
2019; 17C: 576–582] https://10.1016/j.bcab.2019.01.022 Declaration of competing
interest: The authors declare that they have no known competing financial interests
or personal relationships that could have appeared to influence the work reported
in this paper.13. “Hesperidin inhibits cell proliferation and induces
mitochondrial-mediated apoptosis in human lung cancer cells through down regulation
of β-catenin/c-myc” [Biocatalysis and Agricultural Biotechnology, 2019; 18C:
101065] https://10.1016/j.bcab.2019.101065 Declaration of competing interest: The
paper.14. “One-pot green route synthesis of silver nanoparticles from jack fruit
seeds and their antibacterial activities with escherichia coli and salmonella
bacteria” [Biocatalysis and Agricultural Biotechnology, 2019; 20C: 101241] DOI-
paper.15. “Persister cell development among Enterobacteriaceae, Pseudomonadaceae,
Mycobacteriaceae and Staphylococcaceae biotypes: A review” [Biocatalysis and
Agricultural Biotechnology, 2019; 22C: 101401] https://10.1016/j.bcab.2019.101401
influence the work reported in this paper. © 2020
AN - rayyan-553780016
DO - 10.1016/j.bcab.2020.101872
KW - Biotechnology
PY - 2021
ST - Erratum regarding missing Declaration of Competing Interest statements in
previously published articles (Biocatalysis and Agricultural Biotechnology (2019)
20, (S1878818119307182), (10.1016/j.bcab.2019.101241))
T2 - Biocatalysis and Agricultural Biotechnology
TI - Erratum regarding missing Declaration of Competing Interest statements in
previously published articles (Biocatalysis and Agricultural Biotechnology (2019)
20, (S1878818119307182), (10.1016/j.bcab.2019.101241))
85096511049&doi=10.1016%2fj.bcab.2020.101872&partnerID=40&md5=aab2a9a9fff9ed812514c
1826d196b72
VL - 36
ID - 8057
ER -
TY - JOUR
AB - Aim: The present study was carried out to determine the effects of ZnO
nanoparticles (ZnO-NPs) on intestinal function and pathophysiological alteration.
Materials & methods: ZnO-NPs were synthesized and their characterizations were
performed using various techniques. The Wistar male rats fed with normal diet
and/or high fat diet (HFD) for 8 weeks and then orally received ZnO-NPs (5, 50 and
100 mg/kg bodyweight) for 28 days. The oxidative stress (SOD, CAT, GPx),
inflammatory (TNF-alpha, iNOS) and apoptosis pathways (Bcl2, Bax and p53) genes
expression and protein levels were measured by real-time polymerase chain reaction
and available kit, respectively. The activity of Caspase-3, antioxidant capacity,
as well as inflammatory markers were determined. The histological alterations of
the large and small intestine were also evaluated with haematoxylin and eosin (H&E)
as well as TdT dUTP nick end labeling (TUNEL) assay. The biochemical factors,
viability and antioxidant activity were also determined in Caco-2 cells. Results:
It was found that the antioxidant enzymes activity and genes expression markedly
increased, while inflammatory and apoptosis pathways and TNF-alpha levels
significantly decreased in the intestine of HFD-fed rats treated with 5 mg/kg ZnO-
NPs. Intestinal morphological changes were also restored by 5 mg/kg ZnO-NPs in HFD
group. Conclusion: Treatment of rats with 50 and 100 mg/kg ZnO-NPs significantly
induced intestinal injury, while treatment with 5 mg/kg ZnO nanoparticle normalized
intestinal functions and structure. This study showed the synergistic effects of
ZnO-NPs and HFD administration on liver enzyme, oxidative stress, apoptosis,
inflammation, morphological changes and cell toxicity.
AN - rayyan-553780018
AU - Abbasi-Oshaghi, E.
AU - Mirzaei, F.
AU - Mirzaei, A.
DO - 10.2217/nnm-2018-0202
IS - 21
KW - Rats
PY - 2018
SN - 1743-5889 1748-6963
SP - 2791-2816
ST - Effects of ZnO nanoparticles on intestinal function and structure in
normal/high fat diet-fed rats and Caco-2 cells
T2 - NANOMEDICINE
TI - Effects of ZnO nanoparticles on intestinal function and structure in
normal/high fat diet-fed rats and Caco-2 cells
VL - 13
Y2 - 11
ID - 8059
ER -
TY - JOUR
AB - Purpose: This study evaluated the effects of titanium dioxide nanoparticles
(TiO2 NPs) on liver and intestine of normal rats. Methods: Male rats were divided
into four groups as follows: 1) control rats, 2) control rats that orally received
10 mg/kg TiO2 NPs, 3) control rats that orally received 50 mg/kg TiO2 NPs, and 4)
control rats that orally received 100 mg/kg TiO2 NPs. After 30 days, the NLRP3
inflammasome pathway (NLRP3, caspase-1, and IL-1 beta), antioxidant pathway
(superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT]),
inflammatory pathway (inducible nitric oxide synthase [iNOS] and tumor necrosis
factor-alpha [TNF-alpha]), and the apoptosis pathway (p53, Bax, Bcl-2, and caspase-
3) were determined in the intestine and liver of the rats. H&E and Masson's
trichrome (MT) staining as well as TUNEL assay were used to examine the liver and
the intestine. Biochemical factors, cytotoxicity, ROS generation, and apoptosis
rate were also determined in HepG2 and Caco-2 cells. Results: TiO2 NPs in a dose-
dependent manner increased cytotoxicity, oxidative stress, and apoptosis rate in
Caco-2 and HepG2 cells. The administration of TiO2 NPs significantly reduced
antioxidant enzyme activity and gene expressions (SOD, CAT, and GPx) as well as
glutathione (GSH) levels and total antioxidant capacity (TAC) in a dose-dependent
manner. TiO2 NPs also induced the apoptosis pathway and inflammatory pathway gene
expressions and caspase-3 activity in the intestine and liver. TUNEL assay was in
agreement with gene expressions. TiO2 NPs also led to morphological changes in the
liver and intestine. Conclusion: TiO2 NPs could have cytotoxic effects on the
intestine and liver structure and function by inducing oxidative stress,
inflammation, and apoptosis.
AN - rayyan-553780019
AU - Abbasi-Oshaghi, E.
AU - Mirzaei, F.
AU - Pourjafar, M.
DO - 10.2147/IJN.S192382
KW - Titanium
Oxidative Stress
Apoptosis
Rats
Liver
PY - 2019
SN - 1178-2013
SP - 1919-1936
ST - NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine
and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro
study
T2 - INTERNATIONAL JOURNAL OF NANOMEDICINE
TI - NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine
and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro
study
VL - 14
ID - 8060
ER -
TY - JOUR
AB - The biosynthesis of metal nanoparticles using plant extracts is an eco-
friendly and inexpensive solution that has strong potential and applications in
science and industry. This study aims to synthesize Cu, Ag, and Au monometallic and
trimetallic nanoparticles (NPs) using the extracted polysaccharides (PS) of Vossia
cuspidata (Roxb.) Griff. leaves. Besides, the anti-cancer, anti-inflammatory, and
wound healing potentials of the synthesized NPs were tested. The synthesized NPs
were characterized using standard technological methods. We succeeded in green
synthesizing CuO, Ag, Au, monometallic, and CuO-Ag-Au trimetallic NPs. The
synthesized NPs had weak cytotoxicity at low concentrations (6.5 mu g/ml), but the
viability of cancer cells was reduced by increasing the concentration, suggesting
that the synthesized NPs have potent anti-cancer properties against the cells. The
synthesized NPs had 19.44-45.9 mu g/ml cytotoxic activity (IC50) against the MCF-7
cell line, 16.50-51.92 mu g/ml against A549, and 115.90-165.9 mu g/ml for normal
lung cells (WI-38). TMNPs were the most effective cytotoxic agents against all the
tested cell lines, followed by AuNPs on MCF-7 and CuONPs on A549. The cotton
fabric-treated TMNPs and CuONPs exhibited anti-inflammatory properties greater than
fabric-treated AgNPs and AuNPs and showed the highest odema inhibition (84.61% and
79.28%, respectively). In the wound healing assay, CuONPs and TMNPs caused the
highest percentages of inhibition (87.82% and 61.98%, respectively) for the wound
compared to AgNPs and AuNPs. TMNPs and CuONPs were more efficient in restoring the
tissue integrity of wounds than AgNPs and AuNPs. Accordingly, we recommend using
TMNPs and CuONPs in the wound healing dressings.
AN - rayyan-553780021
AU - Abd El-Aziz, S. M.
AU - Farahat, E. A.
DO - 10.1007/s10904-023-02542-x
IS - 3
KW - Polysaccharides
Wound Healing
Inflammation
PY - 2023
SN - 1574-1443 1574-1451
SP - 853-865
ST - The Activity of Vossia cuspidata Polysaccharides-Derived Monometallic CuO,
Ag, Au, and Trimetallic CuO-Ag-Au Nanoparticles Against Cancer, Inflammation, and
Wound Healing
T2 - JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
TI - The Activity of Vossia cuspidata Polysaccharides-Derived Monometallic CuO,
Ag, Au, and Trimetallic CuO-Ag-Au Nanoparticles Against Cancer, Inflammation, and
Wound Healing
VL - 33
Y2 - 3
ID - 8062
ER -
TY - JOUR
AB - Background: Metals are trace elements, vital in some instances or toxic in
others. Due to this toxicity, they have been used since ancient time as
antimicrobials, and prescribed when plant-only remedies were not efficient enough.
These remedies could still contain secrets that may lead to the discovery of new
therapeutically interesting combinations. The objective of this study was to give a
proof of concept that such remedies combining metals and plants are worth studying
again. Methods: We exploited 4 medical formularies (aqrābādhīn), from three Arab
authors from the 9-12th century. We reproduced a remedy, and analyzed the role of
each ingredient. We further looked for the minimum inhibitory concentration against
three pathogenic bacteria, and we analyzed toxic and inflammatory effects of this
remedy on macrophages. Results: Even if plants were extensively used (almost 80 %
of all ingredients), more than 36 different minerals have been found in these 4
aqrābādhīn. When it came to remedies against infections that could be applied
externally, the use of metals grew to 70 %. We focused on a remedy, containing
mainly metals. We have been able to attribute a role for each ingredient, to show
that this skin remedy helped to combat the infection and to resorb the wound, and
to highlight the mastering of metal transformation by these physicians.
Conclusions: With a very simple recipe, mainly composed of metals, these past
physicians designed a complete and synergistic remedy to combat abscesses, while
restricting the toxic effect of metals to the site of infection. It is a first
example showing that different metal manufactures were evolved to improve their
therapeutic potentials. The knowledge acquired by these physician should deserve
more attention, and unexpected features, original organo-metallic compounds or
therapeutic synergy could still be found from such research. © 2022 Elsevier GmbH
AN - rayyan-553780024
AU - Abdallah, B.
AU - Seguin, C.
AU - Aubert, E.
AU - Ait BenHassou, H.
AU - Sbabou, L.
AU - Choulier, L.
AU - Vonthron, C.
AU - Schalk, I. J.
AU - Mislin, G. L. A.
AU - Fournel, S.
AU - Pitchon, V.
AU - Fechter, P.
DO - 10.1016/j.jtemb.2022.126926
KW - Antimicrobial
Inflammation
Metals
Past pharmacopeia
Wound healing
alkali
ammonia
antimony
antimony sulfide
borate sodium
cadmium
calamine
calcium oxide
calcium sulfate
copper
ferric oxide
gold
lead oxide
lead sulfide
mercury
mercury sulfide
potassium carbonate
potassium nitrate
pyrite
silver
sodium carbonate
sulfur
sulfuric acid
tobramycin
trace element
tumor necrosis factor
unclassified drug
antibacterial activity
antiinflammatory activity
Article
bacteriostatic activity
biotransformation
cell proliferation
cell viability
clinical feature
coughing
cytokine production
cytotoxicity
disk diffusion
drug formulation
Escherichia coli
human
macrophage
minimum inhibitory concentration
nonhuman
proof of concept
Pseudomonas aeruginosa
pustule
pyrolysis
respiratory tract infection
skin abscess
skin fistula
skin infection
skin ulcer
Staphylococcus aureus
thorax pain
tuberculous lymphadenitis
urinary tract infection
zone of inhibition
PY - 2022
ST - Past mastering of metal transformation enabled physicians to increase their
therapeutic potential
T2 - Journal of Trace Elements in Medicine and Biology
TI - Past mastering of metal transformation enabled physicians to increase their
therapeutic potential
85122648783&doi=10.1016%2fj.jtemb.2022.126926&partnerID=40&md5=885afe25544bd30c42b9
b20c2827ffce
VL - 71
ID - 8065
ER -
TY - JOUR
AB - Background The green synthesis of metal and metal oxide nanoparticles (NPs),
notably from plants, has attracted increasing attention in recent years. Although
the increased popularity use of Cymbopogon citratus as a therapeutic substance, to
date, there has not been any research on the chemistry of C. citratus aqueous leaf
extract (ALE) or synthesis of ZnO NPs utilizing an extract from it. The
ecologically safe ALE of C. citratus was employed in this study as a bio-reducing
and capping agent to synthesize ZnO NPs. Results The novelty of the current study
is the investigation of the antioxidant, anti-inflammatory, anti-microbial, and
cytotoxic potencies of biosynthesized ZnO NPs utilizing C. citratus ALE. Zinc
acetate dihydrate was used as the precursor and the leaf extract serves as the
reducing agent. ZnO NPs from ALE of C. citratus were characterized by the spherical
in form by using high-resolution transmission electron microscopy (HR-TEM) and the
Scherrer formula was used to calculate the size of the crystalline structure. The
presence of numerous functional groups in both the ALE and the NPs is confirmed by
FTIR analysis. The highest absorption peak is observed at 370 nm. The stability and
particle size of the biosynthesized ZnO NPs are demonstrated by dynamic light
scattering (DLS) analysis. The biosynthesized ZnO NPs exhibited excellent
antioxidant activity with an IC50 value of 45.67 +/- 0.1 mu g/mL and exerted
interesting anti-inflammatory activity (98.1% +/- 0.04) when compared to the
standard indomethacin (92.1% +/- 0.07) at 1 mg/mL. They also showed anti-microbial
activity for both bacterial and fungal which growth rates for both significantly
decreased with the increase in ZnO NPs concentration compared to the control. The
anticancer activity of biosynthesized ZnO NPs and C. citratus ALE was in vitro
tested against seven human cancer cell lines (HCCL) (i.e. H1299, MDA-MB-468, HNO97,
HEK, HCT116, HuH7, and HEPG2) compared to normal cells (HSF) using the
sulforhodamine-B (SRB) assay. More interestingly, the biosynthesized ZnO NPs
displayed remarkable selective cytotoxicity against all tested cancer cell lines
without any effect on normal cells. In contrast, the cancer cell lines were not
affected by the ALE of C. citratus at any concentrations tested. Conclusions All
the findings confirm that the ZnO NPs biosynthesized in the current work are
promising candidates for a variety of biological activities, and as a result, they
can be helpful to the medical sector, environmental and agricultural applications.
AN - rayyan-553780026
AU - Abdelbaky, A. S.
AU - Mohamed, Amha
AU - Sharaky, M.
AU - Mohamed, N. A.
AU - Diab, Y. M.
DO - 10.1186/s40538-023-00432-5
IS - 1
PY - 2023
SN - 2196-5641
ST - Green approach for the synthesis of ZnO nanoparticles using Cymbopogon
citratus aqueous leaf extract: characterization and evaluation of their biological
activities
T2 - CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
TI - Green approach for the synthesis of ZnO nanoparticles using Cymbopogon
citratus aqueous leaf extract: characterization and evaluation of their biological
activities
VL - 10
Y2 - 7 y3 - 20
ID - 8067
ER -
TY - JOUR
AB - Purpose: Nowadays, there is a dramatic increase in the interest of potential
impact of consumer-relevant engineered nanoparticles on pregnancy.Materials and
methods: This study investigated the possible protective effect of montelukast in
neonatal organ toxicity induced by maternal exposure to silver nanoparticles
(AgNPs) in rats.Results: It was noticed that montelukast reduced serum urea,
creatinine, renal caspase-3 immunoreactivity and IL-1β and increased total
antioxidant capacity, as compared to AgNPs. In kidney and bone tissue, montelukast
reduced oxidative stress parameters and TNF-α level that was increased with AgNPs.
Surprisingly, montelukast administration increased epidermal growth factor (EGF) in
bone and reduced it in kidney. Furthermore, as compared to AgNPs, montelukast
improved histopathological picture of kidney and bone.Conclusions: In conclusion,
montelukast antagonized the biochemical and histopathological changes occurred in
kidneys and bones of rat offspring by maternal exposure to AgNPs, mostly by anti-
oxidant, anti-apoptotic and anti-inflammatory actions with a possible role for EGF.
AN - rayyan-553782154
AU - Abdelzaher, W. Y.
AU - Rofaeil, R. R.
AU - Abdel-Hafez, S. M. N.
AU - Atta, M.
AU - Bahaa El-Deen, M. A.
AU - Ali, D. M.
DO - 10.1080/08923973.2021.1878213
IS - 2
J2 - Immunopharmacol Immunotoxicol
KW - Acetates/*pharmacology/therapeutic use
Animals
Calcification, Physiologic/*drug effects/physiology
Cyclopropanes/*pharmacology/therapeutic use
Epidermal Growth Factor/*metabolism
Female
Kidney/*drug effects/*metabolism/pathology
Leukotriene Antagonists/*pharmacology/therapeutic use
Male
Metal Nanoparticles/toxicity
Oxidative Stress/drug effects/physiology
Pregnancy
Prenatal Exposure Delayed Effects/chemically induced/drug therapy/metabolism
Quinolines/*pharmacology/therapeutic use
Rats
Rats, Sprague-Dawley
Receptors, Leukotriene/metabolism
Silver/toxicity
Sulfides/*pharmacology/therapeutic use
Leukotriene E4
Leukotriene D4
Epidermal Growth Factor
Leukotriene B4
Leukotriene C4
LA - eng
N1 - Department of Pharmacology, Faculty of Medicine, Minia University, El Minia,
Egypt.; Department of Pharmacology, Faculty of Medicine, Minia University, El
Minia, Egypt.; Department of Pharmacology, Faculty of Pharmacy, Deraya University,
Minya, Egypt.; Department of Histology and Cell Biology, Faculty of Medicine, Minia
University, El Minia, Egypt.; Department of Anatomy, Faculty of Medicine, Minia
University, El Minia, Egypt.; Department of Pediatric, Faculty of Medicine, Minia
University, El Minia, Egypt.; Department of Forensic Medicine and Toxicology,
Faculty of Medicine, Minia University, El Minia, Egypt.
PY - 2021
SN - 1532-2513 (Electronic)
SP - 183-191
ST - Ameliorating effect of leukotriene receptor antagonist in multi-organ
toxicity induced in rat offspring, a possible role for epidermal growth factor
T2 - Immunopharmacology and immunotoxicology
TI - Ameliorating effect of leukotriene receptor antagonist in multi-organ
toxicity induced in rat offspring, a possible role for epidermal growth factor
VL - 43
Y2 - 4
ID - 10065
ER -
TY - JOUR
AB - In the present study, fish were exposed to sub‐lethal doses of CuONPs (68.92
± 3.49 nm) (10 mg/L, 20 mg/L, and 50 mg/L) for a long exposure period (25 days).
Compared to the control group (0.0 mg/L CuONPs), a significant dose‐dependent
elevation in blood urea and creatinine val-ues, serum alanine transaminase,
aspartate transaminase, and alkaline phosphatase enzyme activities were evident in
CuONPs‐exposed groups (p < 0.05). Fish exposure to 50 mg/L CuONPs significantly
upregulated the transcription of pro‐inflammatory cytokines (tumor necrosis factor‐
alpha, interleukin‐1beta, interleukin 12, and interleukin 8), heat shock protein
70, apoptosis‐related gene (caspase 3), and oxidative stress‐related (superoxide
dismutase, catalase, and glutathione peroxi-dase) genes in liver and gills of the
exposed fish in comparison with those in the control group (p < 0.05). Moreover,
varying histopathological injuries were noticed in the hepatopancreatic tissues,
posterior kidneys, and gills of fish groups correlated to the tested exposure dose
of CuONPs. In summary, our results provide new insights and helpful information for
better understanding the mechanisms of CuONPs toxicity in Nile tilapia at
hematological, molecular levels, and tissue levels. © 2021 by the authors. Licensee
MDPI, Basel, Switzerland.
AN - rayyan-553780034
AU - Abdel‐latif, H. M. R.
AU - Dawood, M. A. O.
AU - Mahmoud, S. F.
AU - Shukry, M.
AU - Noreldin, A. E.
AU - Ghetas, H. A.
AU - Khallaf, M. A.
DO - 10.3390/ani11030652
IS - 3
KW - CuONPs
Gene transcription
Histopathology
Nile tilapia
Toxicity
alanine aminotransferase
alkaline phosphatase
aspartate aminotransferase
caspase
caspase 3
catalase
copper nanoparticle
copper oxide nanoparticle
creatinine
cytokine
glutathione
glutathione peroxidase
glutathione reductase
glutathione transferase
heat shock protein 70
interleukin 12
interleukin 1beta
interleukin 8
nanoparticle
reactive oxygen metabolite
silver nanoparticle
superoxide dismutase
alanine aminotransferase blood level
animal experiment
animal model
animal tissue
Article
cancer mortality
catfish
Clarias gariepinus
controlled study
DNA fragmentation
enzyme activity
epithelium hyperplasia
fish
gene expression
histopathology
injury
kidney
LC50
liver toxicity
myocarditis
necrosis
nonhuman
Oreochromis niloticus
oxidative stress
scanning electron microscopy
sublethal dose
tissue level
transcription initiation
transmission electron microscopy
urea blood level
zebra fish
zeta potential
Oxidative Stress
Copper
PY - 2021
SP - 1-21
ST - Copper oxide nanoparticles alter serum biochemical indices, induce
histopathological alterations, and modulate transcription of cytokines, hsp70, and
oxidative stress genes in oreochromis niloticus
T2 - Animals
TI - Copper oxide nanoparticles alter serum biochemical indices, induce
histopathological alterations, and modulate transcription of cytokines, hsp70, and
oxidative stress genes in oreochromis niloticus
85101717517&doi=10.3390%2fani11030652&partnerID=40&md5=0c6bde7a528d21c8899e073804ee
e875
VL - 11
ID - 8073
ER -
TY - JOUR
AB - Aim of investigation. To study of possibilities of endoscopic treatment of
vesicourethral reflux in children. Material & methods. During the period from 2002
until 2005 the endoscopic correction of vesicourethral reflux (VUR) have been
performed in 36 children at the age between 1.7-15 y.o. suffering from first,
second and third degrees of VUR (9 children had bilateral VUR). The statistics of
the urethral refluxes was as follows: first degree VUR - 11,1% (five ureters);
seond degree - 62,2% (28 ureters); third degree - 26,7% (12 ureters). In the
capacity of the implantant was used polyacrilamidal biopolymeral highly viscous gel
containing silver ions - "Argiform" (production of "Bioform", Russia). The gel
possesses constant action, does not dissolve, is not rejected by tissues, and does
not tend to migrate in the tissues. The implantation of the gel was being done
under the general anesthesia. The duration of unilateral endoscopic correction did
not exceed 15-20 minutes, and of bilateral correction - 30-40 minutes. The patients
stayed in hospital only one day. In our further work the implantation was being
done without hospitalization. Results. The results were followed up right after the
implantation. The remote results were followed up in 34 patients (94,4%): they were
examined after a long period - more than two years. In 95,3% of cases was achieved
total liquidation of reflux. In two ureters the severity of reflux was diminished
from third to first degree. There were no complications after the implantation of
gel, not a single case of obstructive post-implantation syndrome or aggravation of
inflammatory process in the urinary tract. The obtained data allow to conclude that
the endoscopic correction of VUR in children by means of injected implantation of
biopolymeral gel "Argiform" is noninvasive, takes little time, does not demand long
lasting hospitalization, is highly efficacious, and economically profitable.
AN - rayyan-553780035
AU - Abdullaev, K. I.
AU - Akbarov, T. R.
IS - 1
KW - Endoscopic correction
Vesicourethral reflux
polyacrylamide gel
silver
adolescent
article
biocompatibility
child
clinical article
cost effectiveness analysis
disease exacerbation
disease severity
endoscopic surgery
follow up
general anesthesia
hospitalization
human
non invasive measurement
operation duration
treatment outcome
ureter obstruction
urinary tract inflammation
vesicoureteral reflux
Only Child
Child
PY - 2005
SP - 110-114
ST - Endoscopic treatment of vesicourethral reflux in children
T2 - Azerbaijan Medical Journal
TI - Endoscopic treatment of vesicourethral reflux in children
18744386716&partnerID=40&md5=4db6f2a9419ca52df2d77a8472542b7a
ID - 8074
ER -
TY - GEN
AB - O osso homógeno fresco congelado tem sido utilizado no intuito de substituir
o osso autógeno. No entanto as suas propriedades de osteoindução e até mesmo
osteocondução não estão bem conceituadas na literatura científica. Esse trabalho
teve por objetivo avaliar amostras de tecido ósseo homógeno enxertados em humanos
através das análises qualitativas histológica e imunoistoquímica. Para tal, dez
pacientes previamente selecionados foram submetidos à cirurgia de reconstrução de
defeitos ósseos em espessura, usando enxerto ósseo homógeno em bloco fresco
congelado estabilizado e fixado por meio de parafusos bicorticais. Após período de
integração de seia meses foi realizado procedimento de reabertura para instalação
dos implantes osseointegráveis. Neste momento cirúrgico, amostras do enxerto ósseo
foram removidas por meio de broca trefina. As amostras foram fixadas em formol 10%,
levadas ao processamento para corte descalcificado em parafina e coradas por
hematoxilina e eosina. Realizou-se processamento imunoistoquímico para a expressão
da enzima Caspase 3. As lâminas foram levadas à microscopia óptica para realização
das análises histológica e imunoistoquímica qualitativas. Os resultados mostraram
tecido ósseo não vital, com poucas áreas de deposição de osso neoformado sobre a
matriz amorfa, presença de infiltrado inflamatório com áreas de osteomielite e
imunomarcação expressiva da enzima Caspase 3. Diante da metodologia empregada e dos
resultados obtidos concluiu-se que o enxerto homógeno apresentou somente a
propriedade biológica de osteocondução durante a fase de incorporação de seis meses
Allogeneic bone has been used fresh frozen in order to replace bone autograft.
However, the properties of osteoinduction and osteoconduction are not even well-
regarded in the scientific literature. This work aimed to evaluate samples of
homogenous bone grafts in humans by qualitative histological analysis and
immunohistochemistry. For this ten pre-selected patients underwent surgical
reconstruction of bone defects in thick, homogenous bone graft using fresh frozen
block stabilized and fixed by bicortical screws. After integration period of six
months reopening procedure was performed for installation of osseointegrated
implants. At this surgical time bone graft samples were removed by means of drill
trephine. The samples were fixed in formalin 10%, taken to court for processing
decalcified paraffin, and stained with hematoxylin and eosin. Immunohistochemistry
was performed processing for the expression of the enzyme Caspase 3. The slides
were brought to light microscopy to carry out the qualitative histology and
immunohistochemistry. The results showed non-vital bone tissue, with few areas of
deposition of new bone formation on the amorphous matrix, presence of chronic
inflammatory infiltrate with areas of osteomyelitis, and immunostaining expressive
of the enzyme Caspase 3. Given the methods employed and the results obtained it was
concluded that the graft showed only a homogenous biological property of
osteoconduction, during the incorporation of six months
AN - rayyan-553780036
AU - Abla, Marcelo Sabbag
KW - Alogenic bone
Biocompatibilidade
Biocompatibility
Bone graft
Bone repair
Enxerto ósseo
Imunoistochemistry
Imunoistoquímica
Osso homógeno
Processo de reparo
LA - pt
PY - 2012
SP - 52-52
ST - Avaliação da incorporação de enxertos ósseos homógenos em humanos: análises
histológica e imunoistoquímica
TI - Avaliação da incorporação de enxertos ósseos homógenos em humanos: análises
histológica e imunoistoquímica
UR - http://www.athena.biblioteca.unesp.br/exlibris/bd/bfo/33004021011P0/2012/
abla_ms_dr_araca.pdf
ID - 8075
ER -
TY - JOUR
AB - O osso homógeno fresco congelado tem sido utilizado no intuito de substituir
o osso autógeno. No entanto, as suas propriedades de osseoindução e até mesmo
osseocondução não estão bem conceituadas na literatura científica. Esse trabalho
teve por objetivo avaliar amostras de tecido ósseo homógeno enxertados em humanos
através das análises qualitativas histológica e imuno-histoquímica. Para tal, dez
pacientes previamente selecionados foram submetidos à cirurgia de reconstrução de
defeitos ósseos em espessura, usando enxerto ósseo homógeno em bloco fresco
congelado estabilizado e fixado por meio de parafusos bicorticais. Após período de
integração de seis meses foi realizado procedimento de reabertura para instalação
dos implantes osseointegráveis. Neste momento cirúrgico, amostras do enxerto ósseo
foram removidas por meio de broca trefina. As amostras foram fixadas em formol 10%,
levadas ao processamento para corte descalcificado em parafina e coradas por
hematoxilina e eosina. Realizou-se processamento imuno-histoquímico para a
expressão da enzima Caspase 3. As lâminas foram levadas à microscopia óptica para
realização das análises histológica e imuno-histoquímica qualitativas. Os
resultados mostraram tecido ósseo não vital, com poucas áreas de deposição de osso
neoformado sobre a matriz amorfa, presença de infiltrado inflamatório com áreas de
osteomielite e imunomarcação expressiva da enzima Caspase 3. Diante da metodologia
empregada e dos resultados obtidos concluiu-se que o enxerto homógeno fresco
congelado em bloco não incorporou ao leito receptor após período de reparação de
seis meses. Allogeneic, fresh-frozen bone has been used in order to replace bone
autografts. However, its osteoinduction and osteoconduction properties are not
well-defined in the scientific literature. This work aimed to evaluate samples of
homogenous bone grafts in humans by qualitative histological and
immunohistochemical analysis. For this, ten pre-selected patients underwent
surgical augmentation of bone defects. The homogenous fresh frozen block bone graft
was stabilized and fixed by bicortical screws. After six months, the reopening
procedure was performed for installation of osseointegrated implants. At this time
surgical bone graft samples were removed by means of drill trephine. The samples
were fixed in 10% formalin, processed with decalcified paraffin, and stained with
hematoxylin and eosin. Immunohistochemistry was performed for the expression of
Caspase 3 enzyme. The slides were brought to light microscopy for qualitative
histology and immunohistochemistry. The results showed non-vital bone tissue, with
few areas of deposition of new bone formation on the amorphous matrix, presence of
chronic inflammatory infiltrate with areas of osteomyelitis, and expressive
immunolabeling of Caspase 3. Given the methods employed and the results it was
concluded that the allograft fresh-frozen block is not incorporated into the
recipient bed after a healing period of six months
AN - rayyan-553780037
AU - Abla, Marcelo Sabbag
AU - Souza, Francisley Ávila
AU - Aranega, Alessandra Marcondes
AU - Okamoto, Roberta
AU - Garcia Júnior, Idelmo Rangel
AU - Magro Filho, Osvaldo
IS - 6
KW - Biocompatibilidade
Enxerto ósseo
Imuno-histoquímica
Osso homógeno
Processo de reparo
LA - pt
PY - 2012
SN - 1678-6661
SP - 159-165
ST - Avaliação da incorporação de enxerto ósseo homógeno fresco congelado em
humanos. Análises histológica e imuno-histoquímica
T2 - ImplantNews
TI - Avaliação da incorporação de enxerto ósseo homógeno fresco congelado em
humanos. Análises histológica e imuno-histoquímica
UR - https://pesquisa.bvsalud.org/portal/resource/pt/biblio-851006
VL - 9
ID - 8076
ER -
TY - JOUR
AB - Simple Summary The main objective of this study is to assess how eugenol
(Eug) affects AgNP-induced nephrotoxicity in rats. After 30 days of treatment with
AgNPs, rats developed nephrotoxicity, which was characterized by disruptions in the
serum levels of blood urea nitrogen, creatinine, uric acid, the total oxidant
capacity, the total antioxidant capacity, and interfering levels of kidney injury
molecule-1, superoxide dismutase, catalase, reduced glutathione, glutathione
peroxidase, malondialdehyde, tumor necrosis factor-alpha (TNF-alpha), and
interleukin-6 in kidney tissues. These biochemical alterations were accompanied by
the destruction of normal renal architecture, with most renal components shedding
their thickness, diameter, and quantity. Furthermore, P53, Caspase3, and TNF-alpha
immunoreactivity were considerably elevated; however, Bcl-2 immunoreactivity was
reduced. Most biochemical, histological, histomorphometrical, and
immunohistochemical alterations in AgNP-treated rats were reversed by Eug. We may
infer that Eug has a protective effect against AgNP-induced nephrotoxicity. The use
of silver nanoparticles (AgNPs) is expanding. This study evaluates the modulator
effect of eugenol (Eug) on AgNP-induced nephrotoxicity in rats. Sixty male rats
were separated into six groups: control, Eug, AgNPs low-dose, AgNPs high-dose, Eug
+ AgNPs low-dose, and Eug + AgNPs high-dose. After 30 days, kidney function,
antioxidative and proinflammatory status, histopathological, histomorphometrical,
and immunohistochemical assessments were performed. AgNPs markedly induced
oxidative stress in renal tissues, characterized by increased levels of blood urea
nitrogen, creatinine, uric acid, kidney injury molecule-1, the total oxidant
capacity, malondialdehyde, tumor necrosis factor-alpha (TNF-alpha), and
interleukin-6, as well as decreased levels of the total antioxidant capacity,
superoxide dismutase, catalase, reduced glutathione, and glutathione peroxidase.
Moreover, the normal renal architecture was destroyed, and the thickness of the
renal capsules, cortex, and medulla, alongside the diameter and quantity of the
normal Malpighian corpuscles and the proximal and distal convoluted tubules were
decreased. Immunoreactivity for P53, caspase-3, and TNF-alpha reactive proteins
were significantly increased; however, Bcl-2 immunoreactivity was decreased. Eug
reversed most biochemical, histological, histomorphometrical, and
immunohistochemical changes in AgNP-treated animals. This study demonstrated that
nephrotoxicity in AgNP-treated rats was mitigated by an Eug supplementation. Eug's
antioxidant, antiapoptotic, and anti-inflammatory capabilities were the key in
modulating AgNPs nephrotoxicity.
AN - rayyan-553780039
AU - Aboelwafa, H. R.
AU - Ramadan, R. A.
AU - Ibraheim, S. S.
AU - Yousef, H. N.
DO - 10.3390/biology11121719
IS - 12
KW - Rats
PY - 2022
SN - 2079-7737
ST - Modulation Effects of Eugenol on Nephrotoxicity Triggered by Silver
Nanoparticles in Adult Rats
T2 - BIOLOGY-BASEL
TI - Modulation Effects of Eugenol on Nephrotoxicity Triggered by Silver
Nanoparticles in Adult Rats
VL - 11
Y2 - 12
ID - 8078
ER -
TY - CHAP
AB - Zika virus (ZIKV) specifically infects cells in the brain cortex, but it is
unclear if astrocytes, brain microvascular endothelial cells, neurons, pericytes,
and microglia can be infected by ZIKV. Additionally, the mechanisms that underlie
the neuropathogenic effects of the ZIKV remain to be determined. ZIKV crosses the
blood-brain barrier (BBB), which led us to examine the potential neurotoxic effects
on microglia, which are the immune cells of the CNS. The current study examined the
ability of ZIKV to infect HBMVEC, normal human astrocyte (NHA) and human microglia
cells, and the effects of ZIKV on the expression of inflammatory cytokines and the
immunomodulatory and antiviral effects of a curcumin-based silver nanoparticle
(Cur-AgNP) nanoformulation on ZIKV-infected microglial cells in vitro.
Additionally, our study examined the potential mechanisms that may underlie
neuroinflammation associated with ZIKV infection that contributes to ZIKV-
associated neuropathology. Our results indicate that the microglial cells are most
susceptible to the ZIKV and the treatment of microglial cultures with the ZIKV
resulted in a significant increase in inflammatory response as evident by the
increased expression of proinflammatory cytokines. Further, the Cur-AgNP
nanoformulation significantly reduced ZIKV expression in ZIKV-infected microglia
and inhibited the expression of cytokines such as, IL-4, INF-γ, and IL-1β. The ZIKV
activity was associated with increased apoptosis and oxidative stress, and
treatment with Cur-AgNP reduced microglial apoptosis and decreased both reactive
oxygen and nitric oxide species, thereby decreasing neuroinflammatory response. The
Cur-AgNP nanoformulation could thus have clinical utility as a potential antiviral
therapeutic in neuroinflammation associated with ZIKV infection. © 2022 Elsevier
Inc. All rights reserved.
AN - rayyan-553780040
AU - Abou-Jaoude, M.
AU - Sharma, R. K.
AU - Nair, A.
AU - Mammen, M. J.
AU - Aalinkeel, R.
AU - Schwartz, S. A.
AU - Mahajan, S. D.
DO - 10.1016/B978-0-323-99596-2.00013-3
KW - AgNPs
Blood-brain barrier
Curcumin
Cytokines
Immunomodulation
Nanoformulation
Reactive oxygen species
Zika virus
Microglia
PY - 2022
SP - 113-128
ST - Nanotherapy approach to target ZIKA virus in microglia: A case study
T2 - Nanotechnological Applications in Virology
TI - Nanotherapy approach to target ZIKA virus in microglia: A case study
85137572518&doi=10.1016%2fB978-0-323-99596-2.00013-
3&partnerID=40&md5=dc3d685fb598f05274d1ffd23ab39a6f
ID - 8079
ER -
TY - JOUR
AB - BACKGROUND: Colorectal cancer (CRC) is considered as the most common type of
gastrointestinal cancers. Chemotherapy became limited due to the adverse side
effects. Therefore, the most effective Croton tiglium extract was selected to be
incorporated by silver nanoparticles (Ag-NPs) then evaluated against colon cancer
induced by azoxymethane (AOM) in rats. METHODS: Different hematological and
biochemical measurements were quantified in addition to markers of oxidative
stress. Specific tumor and inflammatory markers were assayed. Colonic tissues were
examined histopathologically in addition to immunohistochemistry (IHC). Native
proteins and isoenzymes patterns were electrophoretically assayed beside expression
of Tumor Protein P53 (TP53) and Adenomatous Polyposis Coli (APC) genes in colonic
tissues. RESULTS: It was found that AOM caused significant (P≤0.05) elevation in
the hematological and biochemical measurements. C. tiglium nano-extract restored
these measurements to normalcy. Tumor and inflammatory markers elevated
significantly (P≤0.05) in sera of AOM induced colon cancer group in addition to
increasing peroxidation products with decline in antioxidant enzymes activities in
colon tissues. Nano-extract restored these measurements to normalcy in post-treated
group. Histopathological study revealed that nano-extract minimized severity of
inflammatory reactions in all nano-extract treated groups and prevented anti-
Keratin 20 antibody expression in post-treated group. The lowest similarity index
(SI%) values were noticed with electrophoretic protein (SI=71.43%), lipid
(SI=0.00%) and calcium (SI=75.00%) moieties of protein patterns, catalase
(SI=85.71%), peroxidase (SI=85.71%), α-esterase (SI=50.00%) and β-esterase
(SI=50.00%) isoenzymes in colon cancer group. Furthermore, AOM altered the relative
quantities of total native bands. The nano-extract prevented the alterations that
occurred qualitatively in nano-extract post-treated group and quantitatively in all
nano-extract treated groups. Levels of TP53 and APC gene expression increased in
AOM injected group and nano-extract restored their levels to normalcy in the post-
treated group. CONCLUSION: C. tiglium nano-extract exhibited ameliorative effect
against the biochemical and molecular alterations induced by AOM in nano-extract
post-treated group.
AN - rayyan-553782165
AU - Aboulthana, W. M.
AU - Ibrahim, N. E.
AU - Osman, N. M.
AU - Seif, M. M.
AU - Hassan, A. K.
AU - Youssef, A. M.
AU - El-Feky, A. M.
AU - Madboli, A. A.
DO - 10.31557/APJCP.2020.21.5.1369
IS - 5
J2 - Asian Pac J Cancer Prev
KW - Animals
Azoxymethane/*toxicity
Carcinogens/toxicity
Colonic Neoplasms/chemically induced/*drug therapy/pathology
Croton/*chemistry
Male
Metal Nanoparticles/*administration & dosage/chemistry
Oxidative Stress
Plant Extracts/*pharmacology
Rats
Rats, Wistar
Seeds/*chemistry
Silver/*chemistry
Colonic Neoplasms
LA - eng
N1 - Biochemistry Department, Genetic Engineering and Biotechnology Division,
National Research Centre, Dokki, Giza, Egypt.; Microbial Biotechnology Department,
Genetic Engineering and Biotechnology Division, National Research Centre, Dokki,
Giza, Egypt.; Cell Biology Department, Genetic Engineering and Biotechnology
Division, National Research Centre, Dokki, Giza, Egypt.; Toxicology and Food
contaminants, Food Industry and Nutrition Division, National Research Center,
Dokki, Giza, Egypt.; Biochemistry Department, Genetic Engineering and Biotechnology
Division, National Research Centre, Dokki, Giza, Egypt.; Packaging Materials
Department, National Research Center, Dokki, Giza, Egypt.; Pharmacognosy
Department, Pharmaceutical and Drug Industries Research Division, National Research
Centre, Dokki, Giza, Egypt.; Animal Reproduction and Artificial Insemination
Department, Veterinary Division, National Research Centre, Dokki, Giza, Egypt .
PY - 2020
SN - 2476-762X (Electronic)
SP - 1369-1389
ST - Evaluation of the Biological Efficiency of Silver Nanoparticles
Biosynthesized Using Croton tiglium L. Seeds Extract against Azoxymethane Induced
Colon Cancer in Rats
T2 - Asian Pacific journal of cancer prevention : APJCP
TI - Evaluation of the Biological Efficiency of Silver Nanoparticles
Biosynthesized Using Croton tiglium L. Seeds Extract against Azoxymethane Induced
Colon Cancer in Rats
VL - 21
Y2 - 5 y3 - 1
ID - 10076
ER -
TY - JOUR
AB - The synthesis of reliable biological nanomaterials is a crucial area of study
in nanotechnology. In this study, Emericella dentata was employed for the
biosynthesis of AgNPs, which were then combined with synthesized biochar, a porous
structure created through biomass pyrolysis. The synergistic effects of AgNPs and
biochar were evaluated through the assessment of pro-inflammatory cytokines, anti-
apoptotic gene expression, and antibacterial activity. Solid biosynthesized AgNPs
were evaluated by XRD and SEM, with SEM images revealing that most of the AgNPs
ranged from 10 to 80 nm, with over 70% being less than 40 nm. FTIR analysis
indicated the presence of stabilizing and reducing functional groups in the AgNPs.
The nanoemulsion's zeta potential, hydrodynamic diameter, and particle distribution
index were found to be -19.6 mV, 37.62 nm, and 0.231, respectively. Biochar, on the
other hand, did not have any antibacterial effects on the tested bacterial species.
However, when combined with AgNPs, its antibacterial efficacy against all bacterial
species was significantly enhanced. Furthermore, the combined material
significantly reduced the expression of anti-apoptotic genes and pro-inflammatory
cytokines compared to individual treatments. This study suggests that low-dose
AgNPs coupled with biochar could be a more effective method to combat lung cancer
epithelial cells and pathogenic bacteria compared to either substance alone.
AN - rayyan-553780042
AU - Abu Hajleh, M. N.
AU - Al-limoun, M.
AU - Al-Tarawneh, A.
AU - Hijazin, T. J.
AU - Alqaraleh, M.
AU - Khleifat, K.
AU - Al-Madanat, O. Y.
AU - Al Qaisi, Y.
AU - AlSarayreh, A.
AU - Al-Samydai, A.
AU - Qaralleh, H.
AU - Al-Dujaili, E. A. S.
DO - 10.3390/molecules28124757
IS - 12
KW - Lung Neoplasms
Lung
Bacteria
PY - 2023
SN - 1420-3049
ST - Synergistic Effects of AgNPs and Biochar: A Potential Combination for
Combating Lung Cancer and Pathogenic Bacteria
T2 - MOLECULES
TI - Synergistic Effects of AgNPs and Biochar: A Potential Combination for
Combating Lung Cancer and Pathogenic Bacteria
VL - 28
Y2 - 6
ID - 8081
ER -
TY - JOUR
AB - Intrapleural talc is used to produce pleurodesis in malignant pleural
effusions. Prior in vivo studies have documented an acute inflammatory response to
talc in the pleural space but the cellular source of cytokines has not been
identified. The aim of this study was to investigate the acute response of rabbit
pleural mesothelial cells challenged with talc used for pleurodesis and compare it
to prior studies of the response to talc in the rabbit pleural space. Cultured
rabbit pleural mesothelial cells (PMC) were exposed to talc (25 mu g/cm(2)) for 6,
24, or 48 h and assessed for viability, necrosis, and apoptosis by flow cytometry,
Trypan Blue exclusion, and immunocytochemistry, and for the production of
interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), and transforming
growth factor-beta(1) (TGF-beta(1)) by ELISA. More than 50% of the PMC remained
viable 48 h after talc stimulation. The PMC that were nonviable were identified as
either apoptotic or necrotic, with roughly 20% in each category over the 48 h. At 6
h, the IL-8, VEGF, and TGF-beta(1) levels produced by talc-exposed PMC increased
significantly and remained elevated for up to 48 h. These cytokine levels rose at
similar times and at the same or higher levels than have been measured in the
rabbit pleural space in prior studies. We report that viable, talc-exposed, pleural
mesothelial cells may actively mediate the primary inflammatory pleural response in
talc-induced pleurodesis.
AN - rayyan-553780044
AU - Acencio, M. M. P.
AU - Vargas, F. S.
AU - Marchi, E.
AU - Carnevale, G. G.
AU - Teixeira, L. R.
AU - Antonangelo, L.
AU - Broaddus, V. C.
DO - 10.1007/s00408-007-9041-y
IS - 6
KW - Pleurodesis
PY - 2007
SN - 0341-2040 1432-1750
SP - 343-348
ST - Pleural mesothelial cells mediate inflammatory and profibrotic responses in
talc-induced pleurodesis
T2 - LUNG
TI - Pleural mesothelial cells mediate inflammatory and profibrotic responses in
talc-induced pleurodesis
VL - 185
Y2 - 12
ID - 8083
ER -
TY - JOUR
AB - Introduction: Polymethylmethadylate (PMMA) acrylic resins are used to make
dentures for edentulous patients. Objective: To find out the prevalence of Candida
species in patients with and without removable prostheses from a dental clinic in
Leon, Guanajuato, as well as to assess the antifungal effect and biological
behavior of an experimental PMMA with silver nanoparticles for its possible
application in prostheses. Method: To identify Candida species, smear samples were
obtained from the palatal mucosa of 140 patients aged 60 years. The experimental
PMMA with silver nanoparticles was placed in Candida albicans cultures, which were
stained with the Live/Deae kit for analysis under confocal microscopy;
subsequently, it was implanted in Wistar rats in order to know its behavior in the
surrounding tissues. Results: Candida albicans was the most prevalent species in
the evaluated patients, followed by Candida tropicalis and Candida krusei. The
acrylic resin with silver nanoparticles significantly decreased the presence of
Candida albicans. In the animal model, a discrete and controlled inflammatory
reaction was found, which indicated biocompatibility of the acrylic resin that was
used. Conclusions: It is possible for the nanostructured material with antifungal
effect to be used in order to promote the reduction of oral Candida infections in
edentulous patients.
AN - rayyan-553780047
AU - Acosta-Torres, L. S.
AU - Flores-Arriaga, J. C.
AU - Serrano-Diaz, P. N.
AU - Gonzalez-Garcia, I. A.
AU - Viveros-Garcia, J. C.
AU - Villanueva-Vilchis, M. D.
AU - Villanueva-Sanchez, F. G.
AU - Garcia-Contreras, R.
AU - Arenas-Arrocena, M. C.
DO - 10.24875/GMM.20000915
IS - 4
KW - Biocompatible Materials
Candida
PY - 2021
SN - 0016-3813
SP - 437-442
ST - Antifungal biomaterial for reducing infections caused by Candida albicans in
edentulous patients
T2 - GACETA MEDICA DE MEXICO
TI - Antifungal biomaterial for reducing infections caused by Candida albicans in
edentulous patients
VL - 157
Y2 - 7
ID - 8084
ER -
TY - JOUR
AB - Ethnopharmacological relevance: Sphenocentrum jollyanum is a flowering plant
of the Menispermaceae family with bright yellow roots and wedged-shaped leaves. The
plant is reputed to possess exceptional wound healing properties and used in
folkloric medicine to dress chronic wounds. Aim of the study: Wound repair in a
hyperglycemic state is known to be impaired and delayed making treatment a
difficult challenge. This study sought how the aqueous extracts of root and leaf of
Sphenocentrum jollyanum facilitated wound healing by modulating pro-inflammatory
cytokines, vascular endothelial growth factor and microbial colonization on
excision wound created in diabetic rats. Methods: Diabetes (blood glucose >250
mg/dl) was induced by feeding normal rats with high fat diet for 14 days after
which intraperitoneal injection of low dose streptozotocin (35 mg/kg b.w.) was
administered. Wounds were subsequently created and treatments administered
afterwards for 14 days. Results: Administration of Sphenocentrum jollyanum root and
leaf extracts both orally and topically (100 and 200 mg/kg b.w) significantly (p <
0.05) reduced secretion of pro-inflammatory cytokines (TNF-α, IL-6), number of
microbial colonies (CFU/ml × 102), activity of myeloperoxidase and significantly
increased growth factor secretion on wounds of the diabetic rats. Histological
evaluations of wound tissues of treated diabetic rats revealed matured tissue
granulation, presence of new blood vessels, collagen and fibroblast with fewer
inflammatory cells. Conclusion: The use of Sphenocentrum jollyanum effectively
enhanced wound healing which may be related to constituents identified by GC-MS
analysis and can thus, be suggested as a therapeutic agent for diabetic wound
management. © 2022 Elsevier B.V.
AN - rayyan-553780048
AU - Adeleke, O.
AU - Oboh, G.
AU - Adefegha, S.
AU - Osesusi, A.
DO - 10.1016/j.jep.2022.115266
KW - Angiogenesis
Cytokine
Growth factor
Microbes
Sphenocentrum jollyanum
Wound healing
Animals
Cytokines
Diabetes Mellitus, Experimental
Menispermaceae
Plant Extracts
Rats
Vascular Endothelial Growth Factor A
1 methyl 4 [nitromethyl] 4 piperidinol
4 amino 2 chloropyridine
4 bromo n (piperidinomethyl)phthalimide
antibiotic agent
benzopyrazine(quinoxaline)
chloramphenicol
collagen
cytokine
glucose
interleukin 6
malonaldehyde
myeloperoxidase
plant extract
pyridinemethanol
pyrrolo[3,4 c]pyridine 1,3 dione
RNA
Sphenocentrum jollyanum extract
streptozocin
sulfadiazine silver
unclassified drug
uracil
urea
uric acid
vasculotropin
wound healing promoting agent
vasculotropin A
acute toxicity
animal cell
animal experiment
animal model
animal tissue
Article
bacterial count
controlled study
diabetic patient
diabetic wound
fibroblast
glucose blood level
granulation tissue
in vivo study
inflammatory cell
lipid diet
male
mass fragmentography
microbial colonization
mRNA expression level
nonhuman
pathogen load
plant leaf
plant root
rat
real time polymerase chain reaction
RNA isolation
single drug dose
streptozotocin-induced diabetes mellitus
topical treatment
wound closure
wound healing
wound tissue
animal
experimental diabetes mellitus
metabolism
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor B
Vascular Endothelial Growth Factor D
Endothelial Growth Factors
PY - 2022
ST - Effect of aqueous extract from root and leaf of Sphenocentrum jollyanum
pierre on wounds of diabetic rats: Influence on wound tissue cytokines, vascular
endothelial growth factor and microbes
T2 - Journal of Ethnopharmacology
TI - Effect of aqueous extract from root and leaf of Sphenocentrum jollyanum
pierre on wounds of diabetic rats: Influence on wound tissue cytokines, vascular
endothelial growth factor and microbes
85128566858&doi=10.1016%2fj.jep.2022.115266&partnerID=40&md5=52064f67bbb668f1abb39e
cd3bcd0b1f
VL - 293
ID - 8085
ER -
TY - JOUR
AB - To understand the molecular mechanisms underlying the development of
dyslipidemia and lipodystrophy that occurs after administration of aspartic acid
protease inhibitors, we examined transcriptional profiles using cDNA microarrays in
3T3-L1 adipocytes exposed to 10 micromol/l ritonavir for 2-21 days. The expression
levels of approximately 12,000 transcripts were assessed using the MgU74Av2 mouse
microarray chip. Ritonavir altered gene expression of inflammatory cytokines,
stress response genes localized to endoplasmic reticulum, oxidative stress genes,
apoptosis-related genes, and expression of genes involved in cell adhesion and
extracellular matrix remodeling. Microarray analysis also identified a novel gene
downregulated by ritonavir, Cidea, whose expression levels may affect free-fatty
acid metabolism. These changes suggest a unique, stress-related pattern in
adipocytes induced by chronic exposure to the protease inhibitor, ritonavir.
AN - rayyan-553782246
AU - Adler-Wailes, D. C.
AU - Guiney, E. L.
AU - Koo, J.
AU - Yanovski, J. A.
DO - 10.1038/oby.2008.350
IS - 10
J2 - Obesity (Silver Spring)
KW - 3T3-L1 Cells
Adipocytes/*drug effects/metabolism
Animals
Apoptosis/drug effects/genetics
Cell Adhesion/drug effects/genetics
Cytokines/genetics
Extracellular Matrix/drug effects/genetics
Gene Expression Profiling/methods
Gene Expression Regulation/drug effects
HIV Protease Inhibitors/*pharmacology
HIV-Associated Lipodystrophy Syndrome/genetics/metabolism
Humans
Lipid Metabolism/drug effects/genetics
Mice
Oligonucleotide Array Sequence Analysis
Oxidative Stress/drug effects/genetics
RNA, Messenger/metabolism
Ritonavir/*pharmacology
Time Factors
Transcription, Genetic/*drug effects
Gene Expression
Adipocytes
Ritonavir
LA - eng
N1 - Department of Health and Human Services, Eunice Kennedy Shriver National
Institute of Child Health and Human Development, National Institutes of Health,
Bethesda, Maryland, USA.
PY - 2008
SN - 1930-7381 (Print)
SP - 2379-87
ST - Effects of ritonavir on adipocyte gene expression: evidence for a stress-
related response
T2 - Obesity (Silver Spring, Md.)
TI - Effects of ritonavir on adipocyte gene expression: evidence for a stress-
related response
VL - 16
Y2 - 10
ID - 10156
ER -
TY - JOUR
AB - Apoptotic cell death occurs frequently after cerebral ischemia, although
prevailing necrosis could also be observed in older animals. The mechanisms that
lead to delayed cell death after hypoxic-ischemic injury in the developing brain
are not yet known. With silver staining, we showed increased dying neurons after
ischemia and 4 to 24 hours of reperfusion in 7-day-old rats. Using electron
microscopic studies, we investigated the presence of chromatin condensation.
Injured neurons exhibited 2 different morphological types of apoptosis: Type 1
(aggregation of dense masses of chromatin beneath the intact nuclear membrane)
predominately, and Type 2 (cytoplasmic vacuolization). Electron microscopy analysis
also showed that the fragmented chromatin and neuronal debris were phagocytosed by
surrounding glial cells, and associated with an inflammatory reaction leading to
leukocyte infiltration. These results suggest that the immature brain may be more
prone to apoptotic death with neuronal loss by neighboring cells. This experimental
model may be useful for identifying the biochemical mechanisms that initiate and
mediate Type 1 neuronal apoptosis after ischemia and reperfusion in neonate
rodents.
AN - rayyan-553780051
AU - Aggoun-Zouaoui, D.
AU - Ben-Ari, Y.
AU - Charriaut-Marlangue, C.
DO - 10.1016/S1052-3057(00)07279-7
IS - 1
KW - Cell death
Glial cells
Mitosis
Neuron
animal cell
apoptosis
article
brain hypoxia
brain ischemia
brain maturation
chromatin condensation
female
glia cell
male
nerve cell lesion
nerve cell necrosis
newborn
nonhuman
priority journal
rat
reperfusion
Apoptosis
Rats
Infant, Newborn
PY - 2000
SP - 8-15
ST - Neuronal type 1 apoptosis after unilateral focal ischemia with reperfusion in
the P7 neonatal rat
T2 - Journal of Stroke and Cerebrovascular Diseases
TI - Neuronal type 1 apoptosis after unilateral focal ischemia with reperfusion in
the P7 neonatal rat
0033759887&doi=10.1016%2fS1052-3057%2800%2907279-
7&partnerID=40&md5=5bbc09c572196669afda9b183f32c645
VL - 9
ID - 8088
ER -
TY - JOUR
AB - Iron oxide (Fe3O4) nanoparticles (IONPs) have received much attention for
their utility in biomedical applications such as magnetic resonance imaging, drug
delivery and hyperthermia. Recent studies reported that IONPs induced cytotoxicity
in mammalian cells. However, little is known about the genotoxicity of IONPs
following exposure to human cells. In this study, we investigated the cytotoxicity,
oxidative stress and genotoxicity of IONPs in two human cell lines; skin epithelial
A431 and lung epithelial A549. Prepared IONPs were polygonal in shape with a smooth
surface and had an average diameter of 25 nm. IONPs (25-100 mu g/ml) induced dose-
dependent cytotoxicity in both types of cells, which was demonstrated by cell
viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide) and
lactate dehydrogenase leakage assays. IONPs were also found to induce oxidative
stress in a dose-dependent manner, evident by depletion of glutathione and
induction of reactive oxygen species (ROS) and lipid peroxidation. Comet assay
revealed that level of DNA damage was higher with concentration of IONPs in both
types of cells. Quantitative real-time PCR analysis showed that following the
exposure of cells to IONPs, the expression levels of mRNA of caspase-3 and caspase-
9 genes were higher. We also observed the higher activity of caspase-3 and caspase-
9 enzymes in IONPs treated cells. Moreover, western blot analysis showed that
protein expression level of cleaved caspase-3 was up-regulated by IONPs in both
types of cells. Taken together, our data demonstrates that IONPs have potential to
induce genotoxicity in A431 and A549 cells, which is likely to be mediated through
ROS generation and oxidative stress. This study suggests that genotoxic effects of
IONPs should be further investigated at in vivo level.
AN - rayyan-553780052
AU - Ahamed, M.
AU - Alhadlaq, H. A.
AU - Alam, J.
AU - Khan, M.
AU - Ali, D.
AU - Alarafi, S.
DO - 10.2174/1381612811319370011
IS - 37
KW - Humanities
Humanism
Humans
Oxalic Acid
Iron
DNA Damage
Cell Line
Oxidative Stress
PY - 2013
SN - 1381-6128 1873-4286
SP - 6681-6690
ST - Iron Oxide Nanoparticle-induced Oxidative Stress and Genotoxicity in Human
Skin Epithelial and Lung Epithelial Cell Lines
T2 - CURRENT PHARMACEUTICAL DESIGN
TI - Iron Oxide Nanoparticle-induced Oxidative Stress and Genotoxicity in Human
Skin Epithelial and Lung Epithelial Cell Lines
VL - 19
Y2 - 11
ID - 8089
ER -
TY - JOUR
AB - This study pertains to the new approach for the development of hybrid peptide
LL-37Tα1 and its biomedical applications. A linear cationic hybrid peptide, LL-
37Tα1 was derived from two parental peptides (LL-37 and Tα1) recognized as potent
anti-endotoxin without any hemolytic or cytotoxic activity. We successfully cloned
the gene of hybrid peptide LL-37Tα1 in PpICZαA vector and expressed in the Pichia
pastoris. The recombinant peptide was purified by Ni-affinity column and reverse-
phase high performance liquid chromatography (RP-HPLC) with an estimated molecular
mass of 3.9 kDa as determined by SDS-PAGE and mass spectrometry. We analyzed the
LPS neutralization by limulus amebocyte lysate (LAL) activity and the results
indicate that the hybrid peptide LL-37Tα1 directly binds endotoxin and
significantly (p < 0.05) neutralizes the effect of LPS in a dose-dependent manner.
Lactate dehydrogenase (LDH) assay revealed that LL-37Tα1 successfully reduces the
LPS-induced cytotoxicity in mouse RAW264.7 macrophages. Moreover, it significantly
(p < 0.05) decreased the levels of nitric oxide, proinflammatory cytokines
including TNF-α, IL-6, IL-1β, and diminished the number of apoptotic cells in LPS-
stimulated mouse RAW264.7 macrophages. Our results suggest that the P. pastoris
expression system is cost-effective for commercial production of the
immunomodulatory and anti-inflammatory hybrid peptide (IAHP) LL-37Tα1 and the
peptide may serve as effective anti-endotoxin/anti-inflammatory agent with minimal
cytotoxicity. Copyright © 2019 Ahmad, Hanif, Xubiao, Lulu, Shahid, Dayong and
Rijun. This is an open-access article distributed under the terms of the Creative
Commons Attribution License (CC BY). The use, distribution or reproduction in other
forums is permitted, provided the original author(s) and the copyright owner(s) are
credited and that the original publication in this journal is cited, in accordance
with accepted academic practice. No use, distribution or reproduction is permitted
which does not comply with these terms.
AN - rayyan-553780053
AU - Ahmad, B.
AU - Hanif, Q.
AU - Xubiao, W.
AU - Lulu, Z.
AU - Shahid, M.
AU - Dayong, S.
AU - Rijun, Z.
DO - 10.3389/fimmu.2019.01365
KW - Apoptosis
Hybrid peptides
Immunomodulatory
LPS neutralization
Yeast expression
Animals
Anti-Inflammatory Agents
Antimicrobial Cationic Peptides
Chromatography, High Pressure Liquid
Cloning, Molecular
Cytokines
Fish Proteins
Immunologic Factors
Inflammation Mediators
L-Lactate Dehydrogenase
Lipopolysaccharides
Mice
Nitric Oxide
Pichia
RAW 264.7 Cells
antiinflammatory agent
cathelicidin antimicrobial peptide LL 37
cathelicidin antimicrobial peptide ll 37 t alpha 1
immunomodulating agent
interleukin 1beta
interleukin 6
lipopolysaccharide
restriction endonuclease
unclassified drug
antimicrobial cationic peptide
autacoid
CAP18 lipopolysaccharide-binding protein
cytokine
fish protein
immunologic factor
lactate dehydrogenase
nitric oxide
affinity chromatography
animal cell
apoptosis
Article
controlled study
cytotoxicity
down regulation
drug purification
electrospray mass spectrometry
enzyme linked immunosorbent assay
hemolysis
immunomodulation
Komagataella pastoris
mouse
nonhuman
polyacrylamide gel electrophoresis
polymerase chain reaction
protein expression
RAW 264.7 cell line
reversed phase high performance liquid chromatography
silver staining
animal
genetics
high performance liquid chromatography
metabolism
molecular cloning
C-Peptide
PY - 2019
ST - Expression and purification of hybrid ll-37tα1 peptide in pichia pastoris and
evaluation of its immunomodulatory and anti-inflammatory activities by LPS
neutralization
T2 - Frontiers in Immunology
TI - Expression and purification of hybrid ll-37tα1 peptide in pichia pastoris and
evaluation of its immunomodulatory and anti-inflammatory activities by LPS
neutralization
85068956202&doi=10.3389%2ffimmu.2019.01365&partnerID=40&md5=b1298aa6cdd3fe8ae78c5a7
9f2cb5965
VL - 10
ID - 8090
ER -
TY - JOUR
AB - Nature is a rich source of natural drug-like compounds with minimal side
effects. Phytochemicals better known as "Natural Products" are found abundantly in
a number of plants. Since time immemorial, spices have been widely used in Indian
cuisine as flavoring and coloring agents. Most of these spices and condiments are
derived from various biodiversity hotspots in India (which contribute 75% of global
spice production) and form the crux of India's multidiverse and multicultural
cuisine. Apart from their aroma, flavor and taste, these spices and condiments are
known to possess several medicinal properties also. Most of these spices are
mentioned in the Ayurveda, the indigenous system of medicine. The antimicrobial,
antioxidant, antiproliferative, antihypertensive and antidiabetic properties of
several of these natural products are well documented in Ayurveda. These
phytoconstituemts are known to act as functional immunoboosters, immunomodulators
as well as anti-inflammatory agents. As anticancer agents, their mechanistic action
involves cancer cell death via induction of apoptosis, necrosis and autophagy. The
present review provides a comprehensive and collective update on the potential of
66 commonly used spices as well as their bioactive constituents as anticancer
agents. The review also provides an in-depth update of all major in vitro, in vivo,
clinical and pharmacological studies done on these spices with special emphasis on
the potential of these spices and their bioactive constituents as potential
functional foods for prevention, treatment and management of cancer.
AN - rayyan-553780054
AU - Ahmad, R.
AU - Khan, M. A.
AU - Srivastava, A. N.
AU - Gupta, A.
AU - Srivastava, A.
AU - Jafri, T. R.
AU - Siddiqui, Z.
AU - Chaubey, S.
AU - Khan, T.
AU - Srivastava, A. K.
DO - 10.2174/1871520619666191015103712
IS - 2
PY - 2020
SN - 1871-5206 1875-5992
SP - 122-236
ST - Anticancer Potential of Dietary Natural Products: A Comprehensive Review
T2 - ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
TI - Anticancer Potential of Dietary Natural Products: A Comprehensive Review
VL - 20
ID - 8091
ER -
TY - JOUR
AB - Phytochemicals modulate key cellular signaling pathways and have proven
anticancer effects. Alcea rosea(AR; Hollyhock) is an ornamental plant with known
anti-inflammatory properties. This study explored its role as an anticancer agent.
The AR seed extract (AR extract) inhibited proliferation and colony formation in a
dose- and time-dependent manner and promoted apoptosis as was evidenced by cleavage
of PARP and increased expression of Bax accompanying reduced levels of BCL-xl
protein in HCT116 and SW480 cells, respectively. In addition, AR extract-arrested
cells at Go/G1 phase of cell cycle and exhibited decreases in Cyclin D1. AR
extract-treated cells exhibited reduced number and size of colonospheres in a dose-
dependent manner concomitant with decreases in cancer stem cell (CSC) markers
ALDH1A1 and Dclk1. Relative levels of β-catenin, Notch-ICD, Hes1 and EZH2 were also
attenuated by AR extract. TOP-flash reporter activity, a measure of Wnt signaling,
decreased significantly in response to treatment while overexpression of wild type
but not mutant EZH2, reversed the inhibitory effects. Moreover, WIF1 (a Wnt
antagonist) promoter activity increased dramatically following treatment with AR
extract which phenocopied increases in WIF1 reporter activity following EZH2
knockdown.In vivo, AR extract attenuated tumor growth due probably to reduced
levels of EZH2, β-catenin, CyclinD1 and Ki-67 along with reduced levels of CSC
markers. Since partial purification via HPLC yielded a prominent peak, efforts are
underway to identify the active ingredient(s). Taken together, the results clearly
suggest that AR extract/active component(s) can be an effective
preventative/therapeutic agent to target colon cancer.
AN - rayyan-553782132
AU - Ahmed, I.
AU - Roy, B. C.
AU - Subramaniam, D.
AU - Ganie, S. A.
AU - Kwatra, D.
AU - Dixon, D.
AU - Anant, S.
AU - Zargar, M. A.
AU - Umar, S.
DO - 10.1093/carcin/bgw009
IS - 4
J2 - Carcinogenesis
KW - Colonic Neoplasms/*pathology
*Epigenesis, Genetic
Humans
Neoplastic Stem Cells/*pathology
*Plants
*Signal Transduction
LA - eng
N1 - Department of Molecular and Integrative Physiology, University of Kansas
Medical Center, Kansas City, KS 66160, USA.; Department of Molecular and
Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160,
USA.; Department of Molecular and Integrative Physiology, University of Kansas
Medical Center, Kansas City, KS 66160, USA.; Department of Biochemistry, University
of Kashmir, Srinagar 190006, India.; Center for Drug Evaluation and Research, US
Food and Drug Adminstration, Silver Spring, MD 20993, USA.; Department of Cancer
Biology and.; Department of Molecular and Integrative Physiology, University of
Kansas Medical Center, Kansas City, KS 66160, USA, University of Kansas Cancer
Center, Kansas City, KS 66160, USA.; Department of Biochemistry, University of
Kashmir, Srinagar 190006, India.; Department of Molecular and Integrative
Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA,
University of Kansas Cancer Center, Kansas City, KS 66160, USA sumar@kumc.edu.
PY - 2016
SP - 385-96
ST - An ornamental plant targets epigenetic signaling to block cancer stem cell-
driven colon carcinogenesis
T2 - Carcinogenesis
TI - An ornamental plant targets epigenetic signaling to block cancer stem cell-
driven colon carcinogenesis
VL - 37
Y2 - 4
ID - 10043
ER -
TY - JOUR
AB - The demand for nanoparticles is increasing day by day due to their wide range
of applications in various areas including pharmaceutical industry. Nanoparticles
are formally synthesized by chemical methods in which the toxic and flammable
chemicals are used. Synthesis of nanoparticles from various biological systems has
been reported, but among all, biosynthesis of nanoparticles from plants is
considered as the most suitable method. The current study confirms the potential of
aqueous extract of Melissa officinalis grown under in vitro condition for the green
synthesis of silver nanoparticles (AgNPs). Also, we revealed the cytotoxicity,
antioxidant, and anti-acute myeloid leukemia effects of AgNPs compared to
mitoxantrone in a leukemic mouse model. The synthesized AgNPs were characterized
using several techniques including UV–Vis., FT-IR, TEM, FE-SEM, and EDS. In vivo
experiment, induction of acute myeloid leukemia was done by DMBA in 75 mice. The
obtained results were fed into SPSS-22 software and analyzed by one-way ANOVA. By
quantitative real-time PCR, S1PR1 and S1PR5 mRNA expression in lymphocytes were
significantly (p ≤ 0.01) increased by treating the leukemic mice with the AgNPs and
mitoxantrone. Also, AgNPs similar to mitoxantrone, significantly (p ≤ 0.01)
enhanced the platelet, lymphocyte, and RBC parameters and the anti-inflammatory
cytokines (IL4, IL5, IL10, IL13, and IFNα) and reduced the total WBC, blast,
monocyte, neutrophil, eosinophil, and basophil counts and the pro-inflammatory
cytokines (IL1, IL6, IL12, IL18, IFNY, and TNFα) as compared to the untreated mice.
In vitro experiment, AgNPs similar to mitoxantrone had low cell viability dose-
dependently against murine C1498, human HL-60/vcr, and 32D-FLT3-ITD cell lines
without any cytotoxicity on HUVEC cell line. Furthermore, the DPPH assay showed
similar antioxidant potentials for AgNPs and mitoxantrone. Above results approve
the excellent anti-acute myeloid leukemia, cytotoxicity, and antioxidant properties
of AgNPs compared to mitoxantrone. © 2019 John Wiley & Sons, Ltd.
AN - rayyan-553780058
AU - Ahmeda, A.
AU - Zangeneh, A.
AU - Zangeneh, M. M.
DO - 10.1002/aoc.5378
IS - 2
KW - acute myeloid leukemia
chemotherapeutic drug
Melissa officinalis
mitoxantrone
silver nanoparticles
Antioxidants
Biochemistry
Cell culture
Cytotoxicity
Diseases
Drug delivery
Indicators (chemical)
Lymphocytes
Mammals
Metal nanoparticles
Plant extracts
Polymerase chain reaction
Synthesis (chemical)
Acute myeloid leukemia
Chemical characterization
Chemotherapeutic drugs
Mitoxantrone
Pro-inflammatory cytokines
Quantitative real time PCR
Silver nanoparticles (AgNps)
Silver nanoparticles
Leukemia, Myeloid
Leukemia, Myeloid, Acute
PY - 2020
ST - Preparation, formulation, and chemical characterization of silver
nanoparticles using Melissa officinalis leaf aqueous extract for the treatment of
acute myeloid leukemia in vitro and in vivo conditions
T2 - Applied Organometallic Chemistry
TI - Preparation, formulation, and chemical characterization of silver
nanoparticles using Melissa officinalis leaf aqueous extract for the treatment of
acute myeloid leukemia in vitro and in vivo conditions
85076373188&doi=10.1002%2faoc.5378&partnerID=40&md5=dd2d7a210849b822bb8ca4dec2e72f2
6
VL - 34
ID - 8094
ER -
TY - JOUR
AB - Recent advances in science and technology and greatly modified the way we
stumble on, deal with and prevent special diseases in all components of human
lifestyles. Rheumatoid arthritis (RA) is the most not unusual complex
multifactorial joint related autoimmune, chronic, severe systemic inflammatory
ailment with unknown etiology completed with increased cardiovascular risks. It is
regularly associated with critical synovial joint inflammation, autoantibody
production, cartilage/bone tissue destruction, cardiovascular, pulmonary, skeletal
disorders and massive inflammatory infiltration which might in the end motive
extreme disability, huge complications, premature mortality and decreased life
quality. Pro-inflammatory cytokines like IL-1, IL-6, IL-8 and IL-10 were dependable
for the induction of inflammation in RA patients. It has a global occurrence of
around 1% with the incidence among women being 2-3 times extra in men. Preclinical
RA, genetic variables, and environmental factors have all been linked to the
disease's etiology. Because there is no known cure for RA, the primary goal of
treatment is to achieve the shortest possible illness duration and, if possible,
rehabilitation. Current clinical remedies of RA display numerous drawbacks which
include excessive doses, common administration, speedy metabolism, bad absorption,
low responsiveness, higher cost and serious side consequences. These obstacles have
inspired extremely good growth of the studies and to enhance those obstacles,
nanoparticles that are able to encapsulating and protecting tablets from
degradation earlier than they reach the target site in vivo, might also function
drug delivery structures. Bioavailability and therapeutic bioactivity can be
improved, and limited emphasis on damaged joints can be allowed. The current study
provides a platform for different lipid nanoparticle methods for RA therapy, using
the newly developing field of lipid nanoparticles to improve a targeted theranostic
device for RA treatment. This review aims to present the most recent major
application of lipid nanoparticles as a biocompatible and biodegradable transport
device for improving RA concentration on over free drugs by presenting tissue-
specific concentrated on of ligand-controlled drug release by modulating
nanoparticle composition. Additionally, we also discuss the pivotal demanding
situations to be addressed, as well as destiny views. Therefore, it is feasible to
claim that nanoparticles will, within the near future, play a critical role in
advanced treatment and affected person-particular cures for human diseases which
include RA. © 2021 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
AN - rayyan-553780060
AU - Ahuja, N. K.
AU - Rajawat, J. S.
DO - 10.22159/ijap.2021v13i6.42912
IS - 6
KW - Drug delivery
Inflammation
Lipid nanoparticle
Rheumatoid arthritis
aceclofenac
actarit
antineoplastic agent
autoantibody
C reactive protein
CD40 ligand
celecoxib
curcumin
cyclooxygenase 2
cyclosporine
cystatin C
cytokine
dexamethasone
dexamethasone sodium phosphate
doxorubicin
gelatinase B
glycerol stearate
glycosaminoglycan
hydrogel
interleukin 1
interleukin 10
interleukin 6
interleukin 8
lipid nanoparticle
liposome
macrogol
methotrexate
monocyte chemotactic protein 1
nanocarrier
nanochain
nanocomposite
nanoparticle
piperine
piroxicam
polyglactin
polymer
silver nanoparticle
solid lipid nanoparticle
tacrolimus
antimicrobial activity
autoimmune thyroiditis
bioavailability
biocompatibility
controlled drug release
drug bioavailability
drug delivery system
drug release
encapsulation
environmental factor
human
inflammation
major histocompatibility complex
nanotechnology
nonhuman
Review
rheumatoid arthritis
zeta potential
Arthritis, Rheumatoid
Treatment Outcome
PY - 2021
SP - 31-40
ST - Novel nano therapeutic materials for the effective treatment of rheumatoid
arthritis-recent insights
T2 - International Journal of Applied Pharmaceutics
TI - Novel nano therapeutic materials for the effective treatment of rheumatoid
arthritis-recent insights
85119059996&doi=10.22159%2fijap.2021v13i6.42912&partnerID=40&md5=d88756a71c72ac50ff
21b92dedaf53c2
VL - 13
ID - 8096
ER -
TY - JOUR
AB - By-products from the sugar industry (e.g., blackstrap molasses) can be a
source of bioactive compounds (e.g., phenolics) known to have antimicrobial,
antioxidant, anticancer, and anti-inflammatory properties. These bioactive
molecules can be used in the green synthesis of metal nanoparticles. Metal
nanoparticles have gathered attention because of their novel physico-chemical
properties and potential biological applications (e.g., biocides, fungicides,
pesticides, targeted drug and gene delivery, biosensing, medical implants, and
plant biostimulants). Gold, silver, iron, and copper nanoparticles are of
particular interest as they can be easy to operate and are cost effective, and
biocompatible, and their biological activities can be enhanced by surface
modifications. In this study, the reducing potential of the phenolic compounds in
molasses was investigated for the synthesis of silver nanoparticles without the
external addition of reducing agents. The reddish color formation and peak
appearance at 420 nm were indications of the successful synthesis of the silver
nanoparticles. The synthesized nanoparticles and reducing biomolecules were further
characterized by microscopy (SEM, TEM, EDS) and spectroscopy (FTIR) techniques
indicating nanoparticles of spherical shape and with particle sizes ranging from 15
nm-45 nm. Their antimicrobial activity was evaluated against several Gram-positive
and Gram-negative bacteria. The synthesized nanoparticles showed a biocidal effect,
further confirmed by microscopy techniques. It appears that the nanoparticles are
interacting with the cell surface of bacteria, penetrating the cell and also
causing the disruption of intracellular organelles.
AN - rayyan-553780061
AU - Aita, G. M.
AU - Moon, Y. H.
DO - 10.36961/si29706
IS - 4
PY - 2023
SN - 0344-8657
SP - 206-213
ST - Blackstrap molasses as a source of bioactive compounds for the green
synthesis of nanoparticles
T2 - SUGAR INDUSTRY-ZUCKERINDUSTRIE
TI - Blackstrap molasses as a source of bioactive compounds for the green
synthesis of nanoparticles
VL - 148
Y2 - 4
ID - 8097
ER -
TY - JOUR
AB - This review examines the interaction of nanomaterials (NMs) with cells from
the perspective of major cellular differentiations. The structure and composition
of cells reflect their role and function in a particular organ or environment. The
normal differentiated-state and diseased cells may respond to NMs very differently.
This review progresses with due care on nanotoxicology while emphasizing the
potential of NMs in treating stress associated disorders, including cancer and
degeneration. The striking potential of NMs in inducing ROS, scavenging ROS,
depleting cellular antioxidants, replenishing antioxidants, mimicking antioxidant
enzyme activity, and modulating the immune system all show their considerable
potential in treating cancer and other aging-associated disorders. It is now clear
that NMs become more active and versatile when they come into contact with
biological machinery, surprisingly in some cases, in a manner dependent on cell
type. The mechanisms leading to the contrasting bioresponse of NMs ranging from
toxicity to anticancer and from cell survival to carcinogenicity followed by their
immuno-modulating potential show NMs to be a highly promising agent in biomedical
therapy. This first-of-its-kind article seeks the challenges to be addressed that
could provide a solid rationale in translating the promises of nanomedicine. A
thorough understanding of normal and cancer biology could help to minimize the gap
between basic and translational research in nanotechnology-based therapy.
AN - rayyan-553780062
AU - Akhtar, M. J.
AU - Ahamed, M.
AU - Alhadlaq, H. A.
DO - 10.1016/j.cca.2018.10.004
PY - 2018
SN - 0009-8981 1873-3492
SP - 186-196
ST - Challenges facing nanotoxicology and nanomedicine due to cellular diversity
T2 - CLINICA CHIMICA ACTA
TI - Challenges facing nanotoxicology and nanomedicine due to cellular diversity
VL - 487
Y2 - 12
ID - 8098
ER -
TY - JOUR
AB - SUMMARY: Adriamycin (ADR) is an anthracycline antibiotic used for treatment
of many types of cancer. However, its applications may damage to healthy tissues.
Chloroquine (CLQ) is an anti-inflammatory agent used in treatment of many
inflammation associated diseases such as malaria and rheumatoid arthritis.
Moreover, it is used in the treatment of pneumonia caused by COVID-19. The aim of
this study is to determine possible therapeutic effects of Chloroquine on
Adriamycin-induced testicular toxicity in rats. We investigated the effect of CLQ
on testicular injury caused by ADR. Rats were divided into four groups: Control,
ADR, CLQ, ADR+CLQ. After administrations, animals were sacrificed, and testis
tissues were extracted from the animals for the further examinations.
Histopathological changes in testis tissues were evaluated and TNF-α and IL-6
immunostaining were performed to determine the expression levels of these
cytokines. TUNEL method were used for evaluation of apoptotic index. Moreover,
serum testosterone levels were measured by ELISA assay. We observed that ADR group
showed histopathological deterioration when compared to the Control group and CLQ
treatment ameliorated this damage induced by Adriamycin.An increase in TNF-α and
IL-6 immunoreactivities and in the number of apoptotic cells and a decrease in
serum testosterone levels were determined in the ADR group compared to the Control
and CLQ group. Furthermore, our examinations showed an improvement in testicular
tissue in ADR+CLQ group in terms of these parameters when compared to the ADR
group. We suggest that CLQ can be used as a protective agent to reduce the toxic
effects of Adriamycin as a result of its anti-inflammatory and anti-apoptotic
properties. RESUMEN: La adriamicina (ADR) es un antibiótico de antraciclina que se
usa para el tratamiento de muchos tipos de cáncer. Sin embargo, sus aplicaciones
pueden dañar los tejidos sanos. La cloroquina (CLQ) es un agente antiinflamatorio
que se utiliza en el tratamiento de enfermedades asociadas a la inflamación, tal
como la malaria y la artritis reumatoide. También se utiliza en el tratamiento de
la neumonía causada por COVID-19. El objetivo de este estudio fue determinar los
posibles efectos terapéuticos de la cloroquina sobre la toxicidad testicular
inducida por adriamicina en ratas. Investigamos el efecto de CLQ sobre la lesión
testicular causada por ADR. Las ratas se dividieron en cuatro grupos: Control, ADR,
CLQ, ADR + CLQ. Después de las administraciones, se sacrificaron los animales y se
extrajeron los testículos de los animales para los exámenes adicionales. Se
evaluaron los cambios histopatológicos en los tejidos testiculares y se realizó la
inmunotinción de TNF-α e IL-6 para determinar los niveles de expresión de estas
citocinas. Se utilizó el método TUNEL para la evaluación del índice apoptótico.
Además, los niveles de testosterona en suero se midieron mediante un ensayo ELISA.
El grupo ADR mostró un deterioro histopatológico en comparación con el grupo
Control y observamos que el tratamiento con CLQ mejoró el daño inducido por
Adriamicina. Un aumento en las inmunorreactividades de TNF-α e IL-6 y en el número
de células apoptóticas además de una disminución en los niveles séricos de
testosterona se determinaron en el grupo de ADR en comparación con el grupo de
control y CLQ. Además, nuestros exámenes mostraron una mejora en el tejido
testicular en el grupo ADR + CLQ en términos de estos parámetros en comparación con
el grupo ADR. Sugerimos que CLQ se puede utilizar como agente protector para
reducir los efectos tóxicos de la Adriamicina, gracias a sus propiedades
antiinflamatorias y antiapoptóticas.
AN - rayyan-553780063
AU - Akin, Ali Tugrul
AU - Kaymak, Emin
AU - Öztürk, Emel
AU - Karabulut, Derya
AU - Toluk, Ayse
DO - 10.4067/S0717-95022021000401123
IS - 4
KW - Chemotherapy
Daño de testículo
IL-6
Quimioterapia
TNF-&#945
TNF-a
TUNEL
Testis damage
Apoptosis
Inflammation
Rats
Chloroquine
Doxorubicin
LA - en
PY - 2021
SN - 0717-9367
SP - 1123-1131
ST - Chloroquine ameliorates adriamycin-induced testicular damage by suppressing
the inflammation and apoptosis in rats: a histological, immunohistochemical and
biochemical study
T2 - Int. j. morphol
TI - Chloroquine ameliorates adriamycin-induced testicular damage by suppressing
the inflammation and apoptosis in rats: a histological, immunohistochemical and
biochemical study
UR - http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-
95022021000401123&lng=en&nrm=iso&tlng=en
VL - 39
Y2 - 8 y3 - 1
ID - 8099
ER -
TY - JOUR
AB - Zinc oxide nanoparticles (ZnO NPs) are one of the prominent metal oxide
nanoparticles with significant applications in many industries and research
institutes. Various methods of synthesis have been adopted in the production of ZnO
NPs so as to meet its high demand. The environmental implications and economic
challenges attached to most of the means of ZnO NPs synthesis have resulted in the
quest for other alternatives with environmental and economic benefits.
Interestingly, the biological method of synthesis using plant sources have been
found appropriate for the production of ZnO NPs dues to its numerous health,
environmental, economic, and medicinal benefits. The distinctive features of ZnO
NPs synthesized using plant extracts enhanced its application in agriculture for
the production of fertilizers, pesticides, and fumigants. In the field of medicine
and pharmacy, phytosynthesized ZnO NPs have gained remarkable usage in the
production of disinfectant, antifungal, anticancer, antioxidant, anti-inflammatory
and antidiabetics agents. Despite the enlisted benefits of biosynthesized ZnO NPs,
the difficulties associated with the elucidation of formation mechanism and
reactions still remain unraveled. This review described the summary of the recent
advances in the synthesis, mechanism routes, characterization techniques, and
applications of biosynthesized ZnO NPS in agriculture, medicine, and textile
industries. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
AN - rayyan-553780064
AU - Akintelu, S. A.
AU - Folorunso, A. S.
DO - 10.1007/s12668-020-00774-6
IS - 4
KW - Green synthesis
Nanoparticles
Plant extracts and biomedical applications
Zinc oxide
Agricultural robots
Agriculture
Antifungal agents
Fumigation
Green Synthesis
Medical applications
Medicine
Metal nanoparticles
Metals
Oxide minerals
Pesticides
Plant extracts
Textile industry
ZnO nanoparticles
alkaloid
antioxidant
disinfectant agent
flower extract
metal oxide
nanomaterial
nanoparticle
pesticide
plant extract
saponin
silver nanoparticle
terpenoid
zinc oxide nanoparticle
Anti-inflammatories
Biomedical applications
Characterization techniques
Environmental implications
Formation mechanism
Metal oxide nanoparticles
Methods of synthesis
Zinc oxide nanoparticles
antifungal activity
antineoplastic activity
antioxidant activity
biocompatibility
biosynthesis
Culex tritaeniorhynchus
cytotoxicity
environmental health
Escherichia coli
Euphorbia
human
Laurus nobilis
medical research
medicinal plant
MG-63 cell line
nonhuman
photocatalysis
phytochemistry
plant growth
quince
Review
stomach cancer
textile industry
X ray diffraction
II-VI semiconductors
Plant Extracts
Zinc
PY - 2020
SP - 848-863
ST - A Review on Green Synthesis of Zinc Oxide Nanoparticles Using Plant Extracts
and Its Biomedical Applications
T2 - BioNanoScience
TI - A Review on Green Synthesis of Zinc Oxide Nanoparticles Using Plant Extracts
and Its Biomedical Applications
85088506551&doi=10.1007%2fs12668-020-00774-
6&partnerID=40&md5=05f9760f2039450ac15e75e6fcaaedf2
VL - 10
ID - 8100
ER -
TY - JOUR
AB - Background: Ulcerative colitis (UC) is a debilitating chronic inflammatory
bowel disease that is prevalent in Egypt. Current treatment of UC is expensive and
has serious side effects. Aim of the Work: To evaluate the effect of ginger loaded
nanoparticles (GDNP) compared to ginger extract (GE) as an alternative treatment of
acute UC (AUC) in DSS/ ethanol rat model. Material and Methods: Twenty rats were
used. AUC was induced by administration of 2% synthetic dextran sulfate sodium
(DSS). Rats were divided into 4 groups: control (GP I), AUC (GP II), AUC received
2.5 gm GE (GP III) and AUC received 2.5 gm superparamagnetic@ silver nanoparticles
GDNP (GP VI). Colonic and rectal tissue were assessed histologically using
Hematoxylin and eosin (H&E), Periodic acid- Schiff (PAS), Toluidine blue, and
electron microscopy (EM). Epithelial proliferation and apoptosis were assessed by
anti-KI-67 antibody anti BCL2 antibody. Results: GP III and GP IV showed
improvement with GDNP and GE compared to control. Gross damage scores were as
follows: GPI 0±0, GPII 2.6±1.1, GPIII 1.4±1.1, and GPIV 0±0 with significant
variation (P = 0.000). Cumulative histopathological score of GPI, II, III, and IV
were 0±0, 9.2±3.3, 3±1.6, and 1±1 respectively with significant correlation (P=
0.000). GPIII and GPIV displayed strong positive nuclear and cytoplasmic staining
for KI-67 (GPIII Mean=50±15/ HPF). (GPIV Mean=66±8/ HPF) and BCL2 (Mean=60±16 and
76±9/ HPF) with significant variation (P= 0.000). Conclusion: GDNP has more
potential in treatment of AUC compared to GE. Treatment with GDNPs improved signs,
reduced apoptosis and enhanced repair of AUC more significantly EJH copyright ©
2022. All rights served.
AN - rayyan-553780065
AU - Al-Badawi, M. H.
AU - Waly, N. E.
AU - Eid, M. M.
AU - Soliman, N. A.
DO - 10.21608/ejh.2021.68124.1448
IS - 2
KW - BCL2
ginger
ginger loaded nanoparticles
KI-67
ulcerative colitis
Colitis
PY - 2022
SP - 442-456
ST - Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract
as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis
T2 - Egyptian Journal of Histology
TI - Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract
as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis
85144743574&doi=10.21608%2fejh.2021.68124.1448&partnerID=40&md5=9cc1f5282261130cfe7
cf2f9f1a31cb0
VL - 45
ID - 8101
ER -
TY - JOUR
AB - Both Silver (AgNPs) and gold (AuNPs) nanoparticles are increased being
utilized broadly in many industries and biomedical products to enhance their
performance. However, humans are increasingly being exposed to the two metal-NPs,
which also been shown to be highly potential toxic to mammals. Meta-NPs has been
demonstrated to have the capability to cross biological barriers cell membranes and
subsequently interact with intracellular structures. The present in vivo study
assessed the toxicological potential of AgNPs and AuNPs, in 30 mature male albino
rats, assigned to three groups, to receive intraperitoneal injection of 0.25 mg/kg
b. w of AgNPs (G1) or AuNPs (G2) or vehicle only (G3) daily for 21 days. Liver
function makers, thyroid function hormones, testosterone hormone, inflammatory
biomarkers and plasma proteins as well as histological characteristics were tested.
The results revealed increased liver enzymes indicating liver toxicity and injury.
Liver inflammation was manifested by elevated inflammatory cytokines interleukin-6
(IL-6) and tumour-necrosis factor alpha (TNF-alpha). Thyroid hormones were elevated
indicating hyperthyroidism, while testosterone hormone was diminished indicating
their potential to cause infertility in males. However, the low-dose of AgNPs and
AuNPs has no noticeable changes in histomorphologic picture of liver. In
conclusion, both metal-NPs are potentially toxic, with AgNPs exhibiting a greater
toxicity effect than AuNPs. Toxicity mechanisms include direct cellular injury
(lysis) and induction of oxidative stress. Copyright (C) 2013 - All Rights Reserved
- Pharmacophore
AN - rayyan-553780066
AU - Al-Bishri, W. M.
IS - 1
KW - Animal Shells
Animals
PY - 2018
SN - 2229-5402
SP - 48-55
ST - TOXICITY STUDY OF GOLD AND SILVER NANOPARTICLES ON EXPERIMENTAL ANIMALS
T2 - PHARMACOPHORE
TI - TOXICITY STUDY OF GOLD AND SILVER NANOPARTICLES ON EXPERIMENTAL ANIMALS
VL - 9
Y2 - 1
ID - 8102
ER -
TY - JOUR
AB - Vicia faba seeds are a rich source of polyphenolic, and flavonoid compounds
that have high antioxidant activity. The aqueous extract of V. faba seeds has been
used for the biosynthesis of a nanocomposite (NC) consisting of three different
types of nanoparticles that are deposited onto each other as C@Cu2O@Cu.
Physicochemical, optical, electrical, and morphological properties of the as-
biofabricated C@Cu2O@Cu NC were characterized using different analytical techniques
as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR),
Ultraviolet-visible spectroscopy (UV-Vis), Photoluminescence (PL), Transmission
Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Energy Dispersive X-
Ray (EDX), Raman, Zeta-potential, and water/moisture content). Results confirmed
that V. faba seeds aqueous extract mediated the fabrication of C@Cu2O@Cu NC at high
and pure crystalline nature. The nano-formulation had a face center cubic
crystallographic system at the nano-scale range using Debye-Scherrer's equation
(13.8 nm) in XRD analysis and (20.9 +/- 6.2 nm) using TEM analysis. The
crystallinity index (1.6) with a large surface area and polycrystalline nature were
being investigated. In addition, the biological activity of the synthesized NC was
analyzed. C@Cu2O@Cu NC showed high antiinflammatory activity compared with
diclofenac potassium with IC50 = 213.3 mg/mL. Moreover, the nano-formulation had a
potent antibacterial activity, particularly against Gram-negative bacteria.
Cytotoxicity of the C@Cu2O@Cu NC against MCF7, HCT116, and HepG2 cell lines showed
efficient cytotoxic impact with IC50 = 84.8, 116.1, and 120.5 mg/mL, respectively.
So, the nanocomposite C@Cu2O@Cu may provide a promising platform for the effective
treatment of different types of cancer. (C) 2021 The Author(s). Published by
Elsevier B.V.
AN - rayyan-553780070
AU - Al-Hakkani, M. F.
AU - Hassan, S. H. A.
AU - Saddik, M. S.
AU - El-Mokhtar, M. A.
AU - Al-Shelkamy, S. A.
DO - 10.1016/j.jmrt.2021.07.076
PY - 2021
SN - 2238-7854 2214-0697
SP - 1998-2016
ST - Bioengineering, characterization, and biological activities of C@Cu2O@Cu
nanocomposite based-mediated the Vicia faba seeds aqueous extract
T2 - JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
TI - Bioengineering, characterization, and biological activities of C@Cu2O@Cu
nanocomposite based-mediated the Vicia faba seeds aqueous extract
VL - 14
Y2 - 9
ID - 8106
ER -
TY - JOUR
AB - Gold nanoparticles with tiny sizes and biostability are particularly
essential and are employed in a variety of biomedical applications. Using a
reducing agent and a stabilising agent to make gold nanoparticles has been reported
in a number of studies. Gold nanoparticles with a particle size of 25.31 nm were
synthesized in this study utilising Hylocereus polyrhizus (Red Pitaya) extract,
which functions as a reducing and stabilising agent. The extract of Red Pitaya is
said to be a powerful antioxidant and anti-cancer agent. Because of its substantial
blood biocompatibility and physiological stability, green production of gold
nanoparticles with H. polyrhizus fruit extract is an alternative to chemical
synthesis and useful for biological and medical applications. The formation and
size distribution of gold nanoparticles were confirmed by HPLC, UV-Vis
spectrophotometer, X-ray diffraction (XRD), Dynamic light scattering (DLS), Zeta
potential, Transmission electron microscopy (TEM), Fourier transformed infrared
spectroscopy (FTIR), Energy dispersive X-ray (EDX) and X-ray photoelectron
spectroscopy (XPS). The well-analysed NPs were used in various biological assays,
including anti-diabetic, anti-inflammatory, anti-Alzheimer, and antioxidant (DPPH),
and cytotoxic investigations. The NPs also showed a dose-dependent cytotoxic
activity against HCT-116, HepG2 and MCF-7 cell lines, with IC50 of 100 mg/mL for
HCT-116 cells, 155 mg/mL for HepG2, and for MCF-7 cells the value was 165 mg/mL
respectively. Finally, the outstanding biocompatibility of Au-NPs has led to the
conclusion that they are a promising choice for various biological applications.
(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud
University. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - rayyan-553780071
AU - Al-Radadi, N. S.
DO - 10.1016/j.sjbs.2022.01.001
IS - 4
KW - Cell Line
PY - 2022
SN - 1319-562X 2213-7106
SP - 2836-2855
ST - Biogenic proficient synthesis of (Au-NPs) via aqueous extract of Red Dragon
Pulp and seed oil: Characterization, antioxidant, cytotoxic properties, anti-
diabetic anti-inflammatory, anti-Alzheimer and their anti-proliferative potential
against cancer cell lines
T2 - SAUDI JOURNAL OF BIOLOGICAL SCIENCES
TI - Biogenic proficient synthesis of (Au-NPs) via aqueous extract of Red Dragon
Pulp and seed oil: Characterization, antioxidant, cytotoxic properties, anti-
diabetic anti-inflammatory, anti-Alzheimer and their anti-proliferative potential
against cancer cell lines
VL - 29
Y2 - 4
ID - 8107
ER -
TY - JOUR
AB - The progress in the innovative nanocrystal synthesis process by using
environmentally benign and low-priced nontoxic chemicals, solvents, and renewable
sources remains a challenging task for researchers worldwide. The majority of the
existing synthesis techniques engage in the potentially dangerous, for either human
health or the environment. Current investigation has been centered on green
synthesis pro-cesses to create novel nanomaterials, which are eco-friendly as well
as safer for sustainable marketable feasibility. The current work provides the
green synthesis method for gold nanoparticle (GNPs) synthesis using Commiphora
myrrh (C.myrrh) extract. This simple method includes 6 ml of HAuCl4.3H2O treated
with 4 ml C.myrrh extract having pH 4.5 after 80 min at 25 degrees C temperature.
In this novel method, green synthesized GNPs characterized by UV-Vis, X_ray
diffraction spectroscopy (XRD), zeta potential, fourier transform infrared (FT_IR),
high_resolution transmission electron microscopy (HR_TEM), energy disper-sive X_ray
spectroscopy (EDXA), and dynamic light scattering (DLS). During the development
successful antioxidant assay, the DPPH assay was applied. The cell toxicity of
green synthesized GNPs was evaluated following an MTT assay against HCT-116 (colon
cancer) and MCF-7 (breast cancer). Besides molecular docking in the d-elemene for
inhibitor to VEGFR-2 domain revealed more negative docking score (-3.976) which is
an excellent binding affinity to the C.myrrh@GNP. The synthesized GNPs showed
antidiabetic, antibiotic, and antibacterial properties and anti_inflammatory
inhibition against inhibiting COX-1, and COX-2 enzymes. In addition, molecular
docking by Lindestrene (-3.806) and Furanoeudesma-1,3-dien (-3.912) against COX1
and COX2 respectively showed strong binding affin-ity. The molecular docking study
evidenced the anti-inflammatory and cell toxicity study.(c) 2022 The Author(s).
Published by Elsevier B.V. on behalf of King Saud University. This is an open
access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - rayyan-553780072
AU - Al-Radadi, N. S.
DO - 10.1016/j.jsps.2022.06.028
IS - 9
KW - Polyphenols
PY - 2022
SN - 1319-0164 2213-7475
SP - 1215-1242
ST - Single-step green synthesis of gold conjugated polyphenol nanoparticle using
extracts of Saudi?s myrrh: Their characterization, molecular docking and essential
biological applications
T2 - SAUDI PHARMACEUTICAL JOURNAL
TI - Single-step green synthesis of gold conjugated polyphenol nanoparticle using
extracts of Saudi?s myrrh: Their characterization, molecular docking and essential
biological applications
VL - 30
Y2 - 9
ID - 8108
ER -
TY - JOUR
AB - An approach to synthesizing silver nanoparticles(AgNPs)using a Fusarium
graminarum fungus was established in the present study.These nanoparticles are
identified with the following techniques to confirm the form, size, and other
physical properties of the crystal:UV-Visible Spectroscopy(UV-Vis),where the AgNPs
showed absorbance at 420 nm, while X-Ray Diffraction (XRD)showed diffraction peaks
at(38.05°,4 nm).The experiment consisted of 40 mice divided into two groups, the
first group of 20 mice was considered to be the control animals and the other group
20mice was 21day of treatment with a dose of AgNPs(0.1ml/ day).Microscopic
examination of the kidney section showed lobulated glomeruli, a large area of
hemorrhage, changes in degeneration, and inflammatory cell infiltration. Biometric
changes of the renal corpuscle showed no significant difference between the study
group, while Bowman’s capsule, Proximal, and distal convoluted tubules revealed
increased significant differences between the two groups. © 2020, World
Informations Syndicate. All rights reserved.
AN - rayyan-553780073
AU - Al-Sharqi, S. A. H.
IS - 3
KW - AgNPs
Fusarium graminarum
Image J
Kidney
Toxicity
Mice
PY - 2020
SP - 1029-1035
ST - Histological and biometric study of the effects of fusarium graminarum silver
nanoparticles on the kidney in male albino mice
T2 - Medico-Legal Update
TI - Histological and biometric study of the effects of fusarium graminarum silver
nanoparticles on the kidney in male albino mice
85093113700&partnerID=40&md5=47067abdb9ce6039c40a1dcd9be9de20
VL - 20
ID - 8109
ER -
TY - JOUR
AB - We evaluated the fects of different levels of dietary silver nanoparticle
(AgNP) powder on performance, intestinal microflora, carcass traits and blood
parameters of broiler chickens. Three hundred seven-day-old Ross broiler chicks
were randomly divided into five groups, each group replicated three times with 20
birds per replication. Chickens were fed the basal diet with 2.5, 5, 10 and 20 mg
AgNPs per kg feed. Dietary inclusion of AgNPs improved the final body weight,
cumulative weight gain and feed conversion ratio. The best broiler performance,
carcass traits, and relative organ weights were observed in the group supplemented
with 2.5 ppm AgNPs. Increasing the AgNP dose resulted in a significant decrease in
the caecal lactose positive and enterococci bacteria populations, while
lactobacilli counts were numerically increased. The silver residues in the breast
and thigh muscle significantly increased (p <.05) in a dose-dependent manner.
Dietary inclusion of AgNPs induced dose-dependent lesions in liver, kidney, spleen
and duodenum tissues involving degeneration, necrosis, mononuclear infiltration and
focal aggregation of inflammatory cells. In conclusion, despite its potential
positive impacts on growth performance, carcass traits and caecal microbial
population diversity at a dose of 2.5 ppm, dietary inclusion of AgNPs had the
following negative effects on broilers: 1) silver residues in breast and thigh
muscle, which may result in AgNPs transmission to consumers, and 2) cytotoxicity in
intestinal, liver, spleen and kidney cells in a dose-dependent manner. Therefore,
we suggest the use of lower doses of AgNPs (< 2.5 ppm diet) in poultry production
in the future studies.HIGHLIGHTS Dietary inclusion of silver nanoparticles (AgNPs)
in broiler diets more than 2.5 mg/kg diets had many negative effects represented by
accumulation of silver residue in broiler meat and the possibility of transmission
of nanosilver to consumers. AgNPs had a cytotoxic effect on intestine, liver,
spleen and kidney cells in a dose-dependent manner in broilers and might be harmful
to chicken and human health. Therefore, we do not recommend using AgNPs as a
dietary growth promotor or antibacterial agent in broiler diets and their use and
marketing should be controlled and restricted. © 2022 The Author(s). Published by
Informa UK Limited, trading as Taylor & Francis Group.
AN - rayyan-553780074
AU - Al-Sultan, S. I.
AU - Hereba, A. R. T.
AU - Hassanein, K. M. A.
AU - Abd-Allah, S. M. S.
AU - Mahmoud, U. T.
AU - Abdel-Raheem, S. M.
DO - 10.1080/1828051X.2022.2083528
IS - 1
KW - broilers
carcass traits
histopathology
microflora
Nanoparticles
performance
silver
tissue residues
Chickens
PY - 2022
SP - 967-978
ST - The impact of dietary inclusion of silver nanoparticles on growth
performance, intestinal morphology, caecal microflora, carcass traits and blood
parameters of broiler chickens
T2 - Italian Journal of Animal Science
TI - The impact of dietary inclusion of silver nanoparticles on growth
performance, intestinal morphology, caecal microflora, carcass traits and blood
parameters of broiler chickens
85131380851&doi=10.1080%2f1828051X.2022.2083528&partnerID=40&md5=13aafc626c6938f2df
af9881ce936ef3
VL - 21
ID - 8110
ER -
TY - JOUR
AB - This study was conducted to examine the possible effects of Ag-NPs
synthesized using the olive leaf extract on histopathology and cytogenetic effct in
mice. A total of thirty albino mice aged two months were divided into five
treatment groups as follows: Group 1: served as control was administrated orally
with 0.3 mg/kg bw of normal saline; Group 2 and 3 were administrated orally with
varying doses of Ag-NPs synthesized on olive leaf extract (10 and 100 mg/kg bw,
respectively) for 30 days; Group 4 and 5 were administrated orally with olive leaf
crude extract (300 and 1000 mg/kg bw, respectively) for 30 days. At the end of
experimental period, detection of possible chromosomal aberrations in blood samples
and histopathology (liver, spleen, kidney, uterus and testes) were carried out.
Statistically significant differences (P <= 0.05) were observed for the chromosomal
aberrations in all Ag-NPs groups compared to control and to crude olive leaf
extract groups. Histopathological study revealed various alterations in internal
organs at high dose of Ag-NPs group including: inflammatory reaction, blood
congestion, degeneration, fibrosis, mononuclear cells lesion and necrosis. Slight
changes were identified at both doses of crude olive extract treated groups. Based
on these results, oral administration of Ag-NPs could cause genotoxic and
inflammatory responses in mice and this could be representing a risk to both
environment and human health.
AN - rayyan-553780075
AU - Al-taee, E. H.
DO - 10.36103/ijas.v51i5.1155
IS - 5
KW - Mice
PY - 2020
SN - 0075-0530 2410-0862
SP - 1448-1457
ST - EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON
HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE
T2 - IRAQI JOURNAL OF AGRICULTURAL SCIENCES
TI - EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON
HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE
VL - 51
ID - 8111
ER -
TY - CHAP
AB - Traumatic brain injury (TBI) is a leading cause of mortality and chronic
disability worldwide TBI involves an initial primary phase triggered by an
impactful force to the brain and a subsequent secondary pathological phase. The
secondary phase is characterized by key cellular events such as the release of
calcium ions (Ca2+) and a cascade of inflammatory events such as the impairment of
mitochondrial function, increase in oxidative stress, activation of glial cells,
and impairment of the blood-brain barrier (BBB) causing paracellular leakage. There
is no FDA-approved drug for TBI, but current treatment strategies rely on the
delivery of small and macromolecular therapies to the brain, and these are severely
restricted by the BBB, poor retention, off-target toxicity, and by the complex
pathology of TBI. Therefore, there is a growing need for novel therapeutics for the
diagnosis and treatment of TBI with effective delivery tactics and treatment
paradigms such as nano-engineering nanoparticles (NPs). Nanoparticles sizes range
between 1-100 nm and are engineered to form distinct materials such as lipids,
organic polymers, and silica and metals complexes providing NPs with
characteristics that can mitigate TBI secondary events like BBB breakage,
neuroinflammation, oxidative stress, and mitochondrial dysfunction, leading to
mitigation of TBI pathology. Limitations of NP technology in the treatment of TBI
are related to bioavailability, toxicity load and proinflammatory activity of NPs
in the brain. In this chapter, we discuss nanoparticles (NPs) as novel strategies
for the treatment of TBI and explore their synthesis, mechanisms of action, and
limitations. Understanding the mechanisms and complications of NPs as novel
therapeutic strategies will help guide and improve the design of future TBI
therapies. © 2023 John Wiley & Sons Ltd.
AN - rayyan-553780076
AU - Al-Thani, N.
AU - Haider, M. Z.
AU - Al-Mansoob, M.
AU - Patel, S.
AU - Ahmad, S. M. S.
AU - Kobeissy, F.
AU - Shaito, A.
DO - 10.1002/9781119896258.ch8
KW - Carbon dots
Carbon nanotubules
Fullerenes
Gold nanoparticles
Lipid-based nanoparticles
Nanoparticles and brain injury
Oxidative stress
Polymeric nanoparticles
Reactive oxygen species (ROS)
Traumatic brain injury
Brain
Brain Injuries
PY - 2023
SP - 217-228
ST - Nano-engineering in traumatic brain injury
T2 - Impact of Engineered Nanomaterials in Genomics and Epigenomics
TI - Nano-engineering in traumatic brain injury
85160979667&doi=10.1002%2f9781119896258.ch8&partnerID=40&md5=9a1d6d326390c324e54813
d92ace129f
ID - 8112
ER -
TY - JOUR
AB - Polymerization of deoxy sickle cell hemoglobin (HbS) is well recognized as
the primary event that triggers the classic cycles of sickling/unsickling of
patients red blood cells (RBCs). RBCs are also subjected to continuous endogenous
and exogenous oxidative onslaughts resulting in hemolytic rate increases which
contribute to the evolution of vasculopathies associated with this disease.
Compared to steady-state conditions, the occurrences of vaso-occlusive crises
increase the levels of both RBC-derived microparticles as well as extracellular Hb
in circulation. Common byproduct resulting from free Hb oxidation and from Hb-laden
microparticles is heme (now recognized as damage associated molecular pattern
(DAMP) molecule) which has been shown to initiate inflammatory responses. This
review provides new insights into the interplay between microparticles, free Hb and
heme focusing on Hb's pseudoperoxidative activity that drives RBC's cytosolic,
membrane changes as well as oxidative toxicity towards the vascular system.
Emerging antioxidative strategies that include the use of protein and heme
scavengers in controlling Hb oxidative pathways are discussed.
AN - rayyan-553780077
AU - Alayash, A. I.
DO - 10.1016/j.bcmd.2017.05.009
PY - 2018
SN - 1079-9796 1096-0961
SP - 78-86
ST - Oxidative pathways in the sickle cell and beyond
T2 - BLOOD CELLS MOLECULES AND DISEASES
TI - Oxidative pathways in the sickle cell and beyond
VL - 70
Y2 - 5
ID - 8113
ER -
TY - COMP
AB - The antibacterial effects against Staphylococcus epidermidis of five silver
carboxylate complexes with anti-inflammatory ligands were studied in order to
analyze and compare them in terms of stability (in solution and after exposure to
UV light), and their antibacterial and morphological differences. Four effects of
the Ag-complexes were evidenced by transmission electronic microscopy (TEM) and
scanning electronic microscopy (SEM): DNA condensation, membrane disruption,
shedding of cytoplasmic material and silver compound microcrystal penetration of
bacteria. 5-Chlorosalicylic acid (5Cl) and sodium 4-aminosalicylate (4A) were the
most effective ligands for synthesizing silver complexes with high levels of
antibacterial activity. However, Ag-5Cl was the most stable against exposure UV
light (365 nm). Cytotoxic effects were tested against two kinds of eukaryotic
cells: murine fibroblast cells (T10 1/2) and human epithelial ovarian cancer cells
(A2780). The main objective was to identify changes in their antibacterial
properties associated with potential decomposition and the implications for
clinical applications.
AN - rayyan-553782001
AU - Aldabaldetrecu, M.
AU - Tamayo, L.
AU - Alarcon, R.
AU - Walter, M.
AU - Salas-Huenuleo, E.
AU - Kogan, M. J.
AU - Guerrero, J.
AU - Paez, M.
AU - Azócar, M. I.
CY - Switzerland
DO - 10.3390/molecules23071629
ET - 7
J2 - Molecules
KW - Aminosalicylic Acids/chemistry
Animals
Anti-Bacterial Agents/*chemical synthesis/chemistry/pharmacology
Cell Line
Coordination Complexes/*chemical synthesis/chemistry/pharmacology
Drug Stability
Humans
Mice
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Salicylates/chemistry
Silver/*chemistry
Staphylococcus epidermidis/*drug effects
Staphylococcus epidermidis
Staphylococcus
LA - eng
N1 - Faculty of Chemistry and Biology, University of Santiago de Chile, Av.
Bernardo Ó Higgins 3363, Casilla 40, Correo 33, Estación Central, 9170022 Santiago,
Chile. maialen.aldabaldetrecu@usach.cl.; Facultad de Ingeniería, Instituto de
Ciencias Químicas Aplicadas, Polymers and Macromolecules Center, Universidad
Autónoma de Chile, El Llano Subercaseaux 2801, San Miguel, 9170022 Santiago, Chile.
lauratamayo26@gmail.com.; Faculty of Chemistry and Biology, University of Santiago
de Chile, Av. Bernardo Ó Higgins 3363, Casilla 40, Correo 33, Estación Central,
9170022 Santiago, Chile. romina.alarcon@usach.cl.; Faculty of Chemistry and
Biology, University of Santiago de Chile, Av. Bernardo Ó Higgins 3363, Casilla 40,
Correo 33, Estación Central, 9170022 Santiago, Chile. mariana.walter@usach.cl.;
Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas
y Farmacéuticas, Advanced Center for Chronic Diseases (ACCDiS), Universidad de
Chile, 9170022 Santiago, Chile. edison.salash@gmail.com.; Departamento de Química
Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas,
Advanced Center for Chronic Diseases (ACCDiS), Universidad de Chile, 9170022
Santiago, Chile. mkogan@ciq.uchile.cl.; Faculty of Chemistry and Biology,
University of Santiago de Chile, Av. Bernardo Ó Higgins 3363, Casilla 40, Correo
33, Estación Central, 9170022 Santiago, Chile. juan.guerrero@usach.cl.; Faculty of
Chemistry and Biology, University of Santiago de Chile, Av. Bernardo Ó Higgins
3363, Casilla 40, Correo 33, Estación Central, 9170022 Santiago, Chile.
maritza.paez@usach.cl.; Faculty of Chemistry and Biology, University of Santiago de
Chile, Av. Bernardo Ó Higgins 3363, Casilla 40, Correo 33, Estación Central,
9170022 Santiago, Chile. manuel.azocar@usach.cl.
PY - 2018
ST - Stability of Antibacterial Silver Carboxylate Complexes against
Staphylococcus epidermidis and Their Cytotoxic Effects
T2 - Molecules (Basel, Switzerland)
TI - Stability of Antibacterial Silver Carboxylate Complexes against
Staphylococcus epidermidis and Their Cytotoxic Effects
VL - 23
Y2 - 7 y3 - 4
ID - 9917
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are increasingly being incorporated into
products for their antimicrobial properties. This has resulted in increased human
exposures and the possibility of adverse health effects. Mast cells orchestrate
allergic immune responses through degranulation and release of pre-formed
mediators. Little data exists on understanding interactions of AgNPs with mast
cells and the properties that influence activation and degranulation. Using bone
marrow-derived mast cells and AgNPs of varying physicochemical properties we tested
the hypothesis that AgNP physicochemical properties influence mast cell
degranulation and osteopontin production. AgNPs evaluated included spherical 20 nm
and 110 nm suspended in either polyvinylpyrrolidone (PVP) or citrate, Ag plates
suspended in PVP of diameters between 40-60 nm or 100-130 nm, and Ag nanowires
suspended in PVP with thicknesses <100 nm and length up to 2 mu m. Mast cell
responses were found to be dependent on the physicochemical properties of the AgNP.
Further, we determined a role for scavenger receptor B1 in AgNP-induced mast cell
respons8. Mast cell degranulation was not dependent on AgNP dissolution but was
prevented by tyrosine lcinase inhibitor pretreatment. This study suggests that
exposure to AgNPs may elicit adverse mast cell responses that could contribute to
the initiation or exacerbation of allergic disease. (C) 2014 Elsevier Ltd. All
rights reserved.
AN - rayyan-553780078
AU - Aldossari, A. A.
AU - Shannahana, J. H.
AU - Podila, R.
AU - Brown, J. M.
DO - 10.1016/j.tiv.2014.10.008
IS - 1
KW - Mast Cells
PY - 2015
SN - 0887-2333
SP - 195-203
ST - Influence of physicochemical properties of silver nanoparticles on mast cell
activation and degranulation
T2 - TOXICOLOGY IN VITRO
TI - Influence of physicochemical properties of silver nanoparticles on mast cell
activation and degranulation
VL - 29
Y2 - 2
ID - 8114
ER -
TY - JOUR
AB - Objective. Multinucleated cells are relatively resistant to classic
apoptosis, and the factors initiating cell death and damage in myositis are not
well defined. We hypothesized that nonimmune autophagic cell death may play a role
in muscle fiber damage. Recent reports indicate that TRAIL may induce both NF-kappa
B activation and autophagic cell death in other systems. We undertook this study to
investigate the role of TRAIL in cell death and pathogenesis in vitro and in vivo,
using myositis muscle tissues from humans and mice. Methods. Gene expression
profiling was performed in myositis patient and control muscle specimens.
Immunohistochemistry analysis was performed to confirm the gene array findings. We
also analyzed TRAIL-induced cell death (apoptosis and autophagy) and NF-kappa B
activation in vitro in cultured cells. Results. TRAIL was expressed predominantly
in myositis muscle fibers, but not in biopsy specimens from normal or other
dystrophic-diseased muscle. Autophagy markers were up-regulated in humans with
myositis and in mouse models of myositis. TRAIL expression was restricted to
regenerating/atrophic areas of muscle fascicles, blood vessels, and infiltrating
lymphocytes. TRAIL induced NF-kappa B activation and I kappa B degradation in
cultured cells that are resistant to TRAIL-induced apoptosis but that undergo
autophagic cell death. Conclusion. Our data demonstrate that TRAIL is expressed in
myositis muscle and may mediate both activation of NF-kappa B and autophagic cell
death in myositis. Thus, this nonimmune pathway may be an attractive target for
therapeutic intervention in myositis.
AN - rayyan-553780080
AU - Alger, H. M.
AU - Raben, N.
AU - Pistilli, E.
AU - Francia, D. L.
AU - Rawat, R.
AU - Getnet, D.
AU - Ghimbovschi, S.
AU - Chen, Y. W.
AU - Lundberg, I. E.
AU - Nagaraju, K.
DO - 10.1002/art.30530
IS - 11
KW - Muscle, Skeletal
PY - 2011
SN - 0004-3591
SP - 3448-3457
ST - The Role of TRAIL in Mediating Autophagy in Myositis Skeletal Muscle A
Potential Nonimmune Mechanism of Muscle Damage
T2 - ARTHRITIS AND RHEUMATISM
TI - The Role of TRAIL in Mediating Autophagy in Myositis Skeletal Muscle A
Potential Nonimmune Mechanism of Muscle Damage
VL - 63
Y2 - 11
ID - 8116
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are gaining interest in medical applications for
their prominent antibacterial and antimicrobial potentials. AgNPs possess
remarkable anti-inflammatory and antioxidant activities and enhances wound healing.
The main objective of the current study was to investigate the therapeutic effect
of administration of AgNPs on cisplatin (CP) induced pulmonary inflammation in
rats. Sixty male albino rats were used in this study. Rats were divided into 6
groups (n=10). Group I control group. Group II and III control groups received
AgNPs at doses (5 and 10 ppm). Group IV CP group received CP (2.5 mg/kg). Group V
and VI CP group received AgNPs (5, and 10 ppm). All doses were administered
intraperitoneally once a day for 4 weeks. Oxidative stress and antioxidant status,
inflammatory mediators, fibrogenic as well as apoptotic markers were determined in
lung tissues. The results revealed that rats treated with CP showed remarkable
elevation in lung tissues MDA, TNF-alpha, IFN-gamma, IL-6, CRP, Fibrinogen and P53
levels associated with depression in SOD, GSH and CAT activities. However,
administration of AgNPs (5 or 10 ppm) to CP group resulted in significant
amelioration of the aforementioned parameters in a dose dependent manner.
Histopathological investigation of lung tissues of CP group demonstrated disruption
of normal lung architecture and lung injury. However, treatment with AgNPs revealed
significant improvement in lung tissue against CP-induced inflammatory changes and
lung tissue damage. It could be concluded that AgNPs exert potent cytoprotective
effects via combating oxidative stress, inflammation, fibrogenic and apoptotic
markers and repairing histopathological changes in lung tissues.
AN - rayyan-553780081
AU - Alharbi, H. Y.
AU - Helmi, N. W.
AU - Salem, N. A.
DO - 10.9734/JPRI/2021/v33i46A32888
IS - 46
KW - Rats
Inflammation
Cisplatin
PY - 2021
SN - 2456-9119
SP - 453-463
ST - Possible Protective Effect of Silver Nanoparticles against Cisplatin Induced
Pulmonary Inflammation in Rat Model
T2 - JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL
TI - Possible Protective Effect of Silver Nanoparticles against Cisplatin Induced
Pulmonary Inflammation in Rat Model
VL - 33
ID - 8117
ER -
TY - JOUR
AB - We report that the immunogenicity of colloidal gold nanoparticles coated with
polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus
lividus, can function as a proxy for humans for in vitro immunological studies. To
profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10
mu g mL-1), we applied an extensive nano-scale approach, including particle
physicochemical character-isation involving immunology, cellular biology, and
metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea
urchin physiological coelomic fluid (blood equivalent) results in the formation of
a protein "corona" surrounding the NPs from three major proteins that influence the
hydrodynamic size and colloidal stability of the particle. At the lower
concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic
plasticity based on the biosynthesis of metabolites mediating inflammation and
phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an
immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at
24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives
inflammatory signalling by the phagocytes and the resolution at both the low and
high concentrations of the PVP-AuNPs and provides more details regarding the
immunogenicity of these NPs.
AN - rayyan-553780086
AU - Alijagic, A.
AU - Barbero, F.
AU - Gaglio, D.
AU - Napodano, E.
AU - Benada, O.
AU - Kofronova, O.
AU - Puntes, V. F.
AU - Bastus, N. G.
AU - Pinsino, A.
KW - Vaccination
PY - 2021
SN - 0304-3894 1873-3336
ST - Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin
extracellular molecules induce transient immune activation
T2 - JOURNAL OF HAZARDOUS MATERIALS
TI - Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin
extracellular molecules induce transient immune activation
VL - 402
Y2 - 1 y3 - 15
ID - 8122
ER -
TY - JOUR
AB - Indole-3-carbinol (I3C) is a plant based compound present in vegetables
mostly belonging to cruciferous family. I3C has been shown to possess anticancer,
antioxidant and antiinflammatory properties. Humans are increasingly being exposed
to GNPs due an due to their widespread and increased applications in different
fields. Consistently, recent animal and cell based studies have found them to be
carcinogenic, prooxidant and inflammatory. This study sought to examine the
beneficial effects of I3C against oxidative stress, inflammation and
histopathological changes in liver tissues of rats administered with GNPs. Forty
rats randomly divided into four groups: G1 control G2, rats injected i.p. with a
suspension of GNPs (10 nm in size) (20 mu g/kg body wgt) for 7 days; G3, rats
supplemented orally with I3C (150 mg/kg body wgt) for 7 days; and G4, rats injected
with GNPs along with oral supplementation of I3C. Compared to control, rats
administered with GNPs had significantly increased liver functional markers
including ALT, AST, ALP, total and direct bilirubin and significantly decreased
albumin levels. GNP administered rats also demonstrated increased oxidative stress
and inflammatory markers, MDA, 8-OHdG and IL-6 levels and significantly depleted
activities of antioxidants such as glutathione reductase (GR) and glutathione S-
transferase (GST), and transcription factor, Nrf2 compared to control. GNPs also
exerted marked histological changes in liver tissues. Treatment with I3C
significantly restored the GNP induced changes in the levels of all the studied
parameters and also prevented pathological changes in liver tissues. Molecular
docking studies confirm the interaction of I3C with hepatic cell surface receptor
protein LT3 and thereby blocking GNPs from binding to I3C, confirming above
experimental findings. Collectively, the data demonstrate the hepatoprotective
effects of I3C against GNP-induced adverse effects on liver tissue. These
protective effects of I3C appear to be mediated by its ability to downregulate
oxidative stress and inflammation. Thus dietary intake of plant products rich in
I3C may have beneficial health effects. (C) 2020 Published by Elsevier B.V. on
behalf of King Saud University.
AN - rayyan-553780087
AU - Alkhalaf, M. I.
DO - 10.1016/j.arabjc.2020.09.035
IS - 11
KW - Rats
Indoles
PY - 2020
SN - 1878-5352 1878-5379
SP - 8060-8068
ST - Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced
hepatotoxicity in rats
T2 - ARABIAN JOURNAL OF CHEMISTRY
TI - Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced
hepatotoxicity in rats
VL - 13
Y2 - 11
ID - 8123
ER -
TY - JOUR
AB - Green synthesis of silver nanoparticles has gained great interest among
scientists. In view of this data, we conducted this study to identify the
ameliorative effect of green synthesis of silver nanoparticles using Nigella sativa
extract in diabetic neuropathy induced experimentally. In this study, 50 adult male
albino rats were used and they were randomly divided into five groups; the first
group was the healthy control group, the second group were the diabetic neuropathy
diabetic neuropathy induced, Groups (3-6) diabetic neuropathy induced group and
treated with silver nanoparticles, Nigella sativa extract and green synthesized
silver nanoparticles using Nigella sativa extract respectively. Biochemical
parameters including diabetic, inflammatory and antioxidant biomarkers were
evaluated. Brain histopathology was also performed. Results revealed substantial
rise in glucose, AGE, aldose reductase with insulin reduction in diabetic
neuropathy induced group as compared to healthy control. Also, inflammatory markers
increased significantly in diabetic neuropathy induced group. A remarkable change
in oxidative status was observed in the same group. Furthermore, significant
decline in nitrotyrosin level was observed. Regarding gene expression, we found
significant down regulation in brain TKr A accompanied by upregulation of nerve
growth factor in diabetic neuropathy group comparing with healthy control. Several
treatments for diabetic neuropathy remarkably ameliorate all the investigated
biomarkers. Histological findings are greatly relied on for the results achieved in
this study. Therefore, it can be established that green synthesis of silver
nanoparticles in combination with Nigella sativa extract could be a newly
neuroprotective agents against inflammation and oxidative stress characterizing
diabetic neuropathy through their antidiabetic, anti-inflammatory and anti-oxidants
effects. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud
University.
AN - rayyan-553780088
AU - Alkhalaf, M. I.
AU - Hussein, R. H.
AU - Hamza, A.
DO - 10.1016/j.sjbs.2020.05.005
IS - 9
KW - Diabetic Neuropathies
Antioxidants
PY - 2020
SN - 1319-562X 2213-7106
SP - 2410-2419
ST - Green synthesis of silver nanoparticles by Nigella sativa extract alleviates
diabetic neuropathy through anti-inflammatory and antioxidant effects
TI - Green synthesis of silver nanoparticles by Nigella sativa extract alleviates
diabetic neuropathy through anti-inflammatory and antioxidant effects
VL - 27
Y2 - 9
ID - 8124
ER -
TY - JOUR
AB - Nanosilver is widely used in medicine, industry, and other applications where
it poses a high potential risk for human health, even though little information is
available on its toxicity to vital organs. This study was conducted to determine
the histopathological changes induced in renal tissues by various sizes of silver
nanoparticles (SNPs). Male BALB/C mice were exposed to various sizes of SNPs for 5
weeks. Renal tissue samples from all members of all experimental groups were
subjected to histological processing and histopathological examination. SNPs caused
glomerular and tubular alterations in the form of tubular degeneration, necrosis,
eosinophilia, glomerular shrinkage, and Bowman's capsule thickening. Moreover, SNPs
induced interstitial intertubular regeneration, mononuclear inflammatory cell
infiltration, proteinaceous casting, and fibrocyte proliferation. These results
indicated that smaller particles (10 and 20 nm) were more toxic than the larger
ones (40, 60, and 100 nm). In addition, the cortex was more affected than the
medulla, and the proximal tubules were more affected than the distal ones. The
results of the current investigation reveal that SNPs induce histomorphological
alterations in renal tissues, with size being a key factor in the toxicity of these
particles. © 2016 The Institution of Engineering and Technology.
AN - rayyan-553780089
AU - Almansour, M.
AU - Jarrar, Q.
AU - Battah, A.
AU - Obeidat, F.
AU - Battah, K.
AU - Jarrar, B.
DO - 10.1049/mnl.2016.0388
IS - 12
KW - Health risks
Histology
Silver
Tissue
silver nanoparticle
Experimental groups
High potential
Histopathological examinations
Inflammatory cells
Proximal tubules
Silver nanoparticles (SNPs)
Size effects
Tubular alterations
animal experiment
animal model
animal tissue
Article
Bowman capsule
cell infiltration
cell proliferation
controlled study
eosinophilia
histopathology
kidney parenchyma
kidney proximal tubule
male
mouse
necrosis
nonhuman
Toxicity
PY - 2016
SP - 862-865
ST - Renal histopathological alterations induced by nanosilver toxicity: The size
effect
T2 - Micro and Nano Letters
TI - Renal histopathological alterations induced by nanosilver toxicity: The size
effect
84999040197&doi=10.1049%2fmnl.2016.0388&partnerID=40&md5=fa64f104bd4c2c86c8def6e411
a299b2
VL - 11
ID - 8125
ER -
TY - JOUR
AB - Aim To evaluate bone tissue reactions in rats to an MTA-based endodontic
sealer with and without the addition of various concentrations of C3A or C3A + Ag.
Methodology Bone tissue reactions were evaluated in 45 Wistar rats after 7, 30 and
90 days (n = 5 per period). Three surgical cavities were prepared on the right
femur and filled with 0.2 mL MTA Fillapex, MTA Fillapex + C3A and C3A + Ag at
various concentrations: AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany),
EndoSequence BC (Brasseler USA, Savannah, GA, USA) or no sealer (negative control).
By the end of each experimental period, animals were randomly euthanized. The
samples were histologically processed and analysed using a light microscope. The
presence of inflammatory cells, fibres and hard tissue barrier formation was
evaluated. Data were analysed statistically using nonparametric tests to compare
the differences between groups. Multiple groups were compared using the Kruskal-
Wallis and Mann-Whitney U-tests with a Bonferroni correction at P = 0.05. Results
The inflammatory response significantly decreased from 30 to 90 days (P < 0.05).
Fibre condensation was similar amongst the groups at 07 and 30 days after
intervention (P > 0.05). At 90 days, however, fibres were absent in most specimens
of EndoSequence BC Sealer, AH Plus, MTA Fillapex and the control group, whilst they
were still observed in samples of the modified sealers (P < 0.05). At 90 days, all
specimens of AH Plus, EndoSequence BC Sealer and control group had complete
formation of hard tissue barrier. In the MTA Fillapex group, as well as in the
modified sealers groups, partial deposition of mineralized tissue was noticed.
Conclusion The hypothesis tested that the incorporation of C3A and C3A + Ag
particles to MTA Fillapex would improve bone tissue repair was partially accepted,
since modified MTA Fillapex did not have the same repair potential as the
commercial bioceramic material.
AN - rayyan-553780092
AU - Almeida, L. H.
AU - Gomes, A. P. N.
AU - Gastmann, A. H.
AU - Pole, N. M.
AU - Moraes, R. R.
AU - Morgental, R. D.
AU - Cava, S. S.
AU - Felix, A. O. C.
AU - Peppen, F. G.
DO - 10.1111/iej.13135
IS - 10
PY - 2019
SN - 0143-2885 1365-2591
SP - 1446-1456
ST - Bone tissue response to an MTA-based endodontic sealer, and the effect of the
addition of calcium aluminate and silver particles
T2 - INTERNATIONAL ENDODONTIC JOURNAL
TI - Bone tissue response to an MTA-based endodontic sealer, and the effect of the
addition of calcium aluminate and silver particles
VL - 52
Y2 - 10
ID - 8128
ER -
TY - JOUR
AB - Pre-existing conditions modulate sensitivity to numerous xenobiotic exposures
such as air pollution. Specifically, individuals suffering from metabolic syndrome
(MetS) demonstrate enhanced acute inflammatory responses following particulate
matter inhalation. The mechanisms associated with these exacerbated inflammatory
responses are unknown, impairing interventional strategies and our understanding of
susceptible populations. We hypothesize MetS-associated lipid dysregulation
influences mediators of inflammatory resolution signaling contributing to increased
acute pulmonary toxicity. To evaluate this hypothesis, healthy and MetS mouse
models were treated with either 18-hydroxy eicosapentaenoic acid (18-HEPE), 14-
hydroxy docosahexaenoic acid (14-HDHA), 17-hydroxy docosahexaenoic acid (17-HDHA),
or saline (control) via intraperitoneal injection prior to oropharyngeal aspiration
of silver nanoparticles (AgNP). In mice receiving saline treatment, AgNP exposure
resulted in an acute pulmonary inflammatory response that was exacerbated in MetS
mice. A targeted lipid assessment demonstrated 18-HEPE, 14-HDHA, and 17-HDHA
treatments altered lung levels of specialized pro-resolving lipid mediators (SPMs).
14-HDHA and 17-HDHA treatments more efficiently reduced the exacerbated acute
inflammatory response in AgNP exposed MetS mice as compared to 18-HEPE. This
included decreased neutrophilic influx, diminished induction of inflammatory
cytokines/chemokines, and reduced alterations in SPMs. Examination of SPM receptors
determined baseline reductions in MetS mice compared to healthy as well as
decreases due to AgNP exposure. Overall, these results demonstrate AgNP exposure
disrupts inflammatory resolution, specifically 14-HDHA and 17-HDHA derived SPMs, in
MetS contributing to exacerbated acute inflammatory responses. Our findings
identify a potential mechanism responsible for enhanced susceptibility in MetS that
can be targeted for interventional therapeutic approaches. © 2021 Elsevier Inc.
AN - rayyan-553780095
AU - Alqahtani, S.
AU - Xia, L.
AU - Jannasch, A.
AU - Ferreira, C.
AU - Franco, J.
AU - Shannahan, J. H.
DO - 10.1016/j.taap.2021.115730
KW - Inflammatory resolution
Lipid supplementation
Metabolic syndrome
Nanoparticles
Nanotoxicity
Omega-3 polyunsaturated fatty acids
Specialized pro-resolving mediators
Susceptibility
Animals
Cytokines
Diet, High-Fat
Disease Models, Animal
Docosahexaenoic Acids
Gene Expression Regulation
Hydroxyeicosatetraenoic Acids
Lipid Metabolism
Lung
Male
Metabolic Syndrome
Metal Nanoparticles
Mice, Inbred C57BL
Pneumonia
Signal Transduction
Silver Compounds
14 hydroxy docosahexaenoic acid
17 hydroxy docosahexaenoic acid
18 hydroxy eicosapentaenoic acid
arachidonate 15 lipoxygenase
chemokine
chemokine like receptor 1
CXCL1 chemokine
cytokine
G protein coupled receptor 18
interleukin 1beta
interleukin 6
isoflurane
leucine rich repeat containing G protein coupled receptor 6
macrophage inflammatory protein
receptor
silver nanoparticle
sodium chloride
unclassified drug
unsaturated fatty acid
14-hydroxydocosahexaenoic acid
17-hydroxy-4,7,10,13,15,19-docosahexaenoic acid
18-hydroxy-5,8,11,14-eicosatetraenoic acid
autacoid
docosahexaenoic acid
hydroxyicosatetraenoic acid
metal nanoparticle
silver derivative
animal cell
animal experiment
animal model
animal tissue
Article
bronchoalveolar lavage fluid
controlled study
exposure
gene expression profiling
lipid metabolism
lung injury
lung parenchyma
male
metabolic syndrome X
mouse
neutrophil
nonhuman
pneumonia
protein induction
signal transduction
animal
C57BL mouse
comparative study
complication
disease model
drug effect
gene expression regulation
genetics
lipid diet
lung
metabolism
Mice
Inflammation
PY - 2021
ST - Disruption of pulmonary resolution mediators contribute to exacerbated silver
nanoparticle-induced acute inflammation in a metabolic syndrome mouse model
T2 - Toxicology and Applied Pharmacology
TI - Disruption of pulmonary resolution mediators contribute to exacerbated silver
nanoparticle-induced acute inflammation in a metabolic syndrome mouse model
85116321846&doi=10.1016%2fj.taap.2021.115730&partnerID=40&md5=9e7dff73b487cd9b7cedc
40ff636d221
VL - 431
ID - 8131
ER -
TY - JOUR
AB - Background: Metabolic syndrome (MetS) exacerbates susceptibility to
inhalation exposures such as particulate air pollution, however, the mechanisms
responsible remain unelucidated. Previously, we determined a MetS mouse model
exhibited exacerbated pulmonary inflammation 24 h following AgNP exposure compared
to a healthy mouse model. This enhanced response corresponded with reduction of
distinct resolution mediators. We hypothesized silver nanoparticle (AgNP) exposure
in MetS results in sustained pulmonary inflammation. Further, we hypothesized
treatment with resolvin D1 (RvD1) will reduce exacerbations in AgNP-induced
inflammation due to MetS. Results: To evaluate these hypotheses, healthy and MetS
mouse models were exposed to vehicle (control) or AgNPs and a day later, treated
with resolvin D1 (RvD1) or vehicle (control) via oropharyngeal aspiration.
Pulmonary lung toxicity was evaluated at 3-, 7-, 14-, and 21-days following AgNP
exposure. MetS mice exposed to AgNPs and receiving vehicle treatment, demonstrated
exacerbated pulmonary inflammatory responses compared to healthy mice. In the AgNP
exposed mice receiving RvD1, pulmonary inflammatory response in MetS was reduced to
levels comparable to healthy mice exposed to AgNPs. This included decreases in
neutrophil influx and inflammatory cytokines, as well as elevated anti-inflammatory
cytokines. Conclusions: Inefficient resolution may contribute to enhancements in
MetS susceptibility to AgNP exposure causing an increased pulmonary inflammatory
response. Treatments utilizing specific resolution mediators may be beneficial to
individuals suffering MetS following inhalation exposures. © 2022, The Author(s).
AN - rayyan-553780096
AU - Alqahtani, S.
AU - Xia, L.
DO - 10.1186/s12989-022-00495-6
IS - 1
KW - Chronic inflammation
Failure of resolution
Inflammatory resolution
Lipid supplementation
Metabolic syndrome (MetS)
Nanoparticles (NPs)
Nanotoxicity
Omega-3 polyunsaturated fatty acids
Resolvin D1 (RvD1)
Specialized pro-resolving mediators (SPMs)
Susceptibility
Animals
Cytokines
Docosahexaenoic Acids
Inflammation
Metabolic Syndrome
Metal Nanoparticles
Mice
Pneumonia
Silver
cytokine
resolvin D1
silver nanoparticle
unclassified drug
metal nanoparticle
silver
animal cell
animal experiment
animal model
animal tissue
Article
controlled study
inflammation
lung toxicity
male
mouse
nanotoxicology
neutrophil chemotaxis
nonhuman
pneumonia
risk factor
animal
disease model
metabolism
PY - 2022
ST - Enhanced silver nanoparticle-induced pulmonary inflammation in a metabolic
syndrome mouse model and resolvin D1 treatment
T2 - Particle and Fibre Toxicology
TI - Enhanced silver nanoparticle-induced pulmonary inflammation in a metabolic
syndrome mouse model and resolvin D1 treatment
85135590074&doi=10.1186%2fs12989-022-00495-
6&partnerID=40&md5=9146ab791a0b9762ed4cd30612e9a875
VL - 19
ID - 8132
ER -
TY - JOUR
AB - Background: Silver nanoparticles (AgNPs) are attractive substrates for new
medicinal treatments. Biochar is pyrolyzed biomass. Its porous architecture allows
it to hold and gather minuscule particles, through which nanoparticles can
accumulate in its porous structure. This study examined AgNPs’ antibacterial and
anticancer properties alone and combined with biochar. Methods: The fungus
Emericella dentata was responsible for biosynthesis of AgNPs. The characterization
of AgNPs using STEM images and a Zetasizer was carried out. Accordingly, the
antibacterial and antiproliferation activity of AgNPs and biochar was studied using
MIC and MTT assays, respectively. To evaluate the antiangiogenic and anti-
inflammatory effects of AgNPs with biochar, VEGF and cytokines including TNF alpha,
IL-6 and IL-beta were tested using an ELISA assay. Results: The size of the AgNPs
ranged from 10 to 80 nm, with more than 70% of them being smaller than 40 nm. The
combination of AgNPs and biochar enhanced the antibacterial activity against all
tested bacteria. Furthermore, this combination showed antiproliferative properties
against HT29 cancer cells with high selectivity to fibroblasts at low
concentrations. AgNPs with biochar significantly reduced VEGF and proinflammatory
cytokine expression levels. Conclusions: Biochar and AgNPs may be novel treatments
for bacteria and colorectal cancer cells, according to the current findings. © 2023
by the authors.
AN - rayyan-553780097
AU - Alqaraleh, M.
AU - Khleifat, K. M.
AU - Abu Hajleh, M. N.
AU - Farah, H. S.
AU - Ahmed, K. A. A.
DO - 10.3390/antibiotics12030597
IS - 3
KW - antibacterial
antitumor activity
biochar
colorectal cancer
charcoal
fungal extract
graphene oxide
interleukin 1beta
interleukin 6
protein p53
silver nanoparticle
vasculotropin
animal cell
antifungal activity
apoptosis
Article
bacterial growth
bacterium isolation
broth dilution
cell proliferation
cell viability
circular dichroism
colorectal cancer cell line
cytotoxicity assay
disk diffusion
DNA damage
DNA fragmentation
fibroblast
gene expression
HT-29 cell line
human
human cell
hydrophobicity
IC50
MTT assay
nonhuman
nucleotide sequence
oxidative stress
photon correlation spectroscopy
protein expression
ultraviolet spectroscopy
zeta potential
Colorectal Neoplasms
PY - 2023
ST - Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal
Cancer Cells and Pathogenic Bacteria
T2 - Antibiotics
TI - Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal
Cancer Cells and Pathogenic Bacteria
85151741470&doi=10.3390%2fantibiotics12030597&partnerID=40&md5=29d87a14b5061f037e5d
d7270cf27101
VL - 12
ID - 8133
ER -
TY - CHAP
AB - Mast cells are key effector cells in inflammatory and allergic immune
responses such as asthma, rhinitis, and atopic dermatitis. Activation of mast cells
leads to immediate release of preformed mediators such as histamine and proteases,
which can regulate vascular permeability and the function of a number of immune and
nonimmune cells. Engineered nanomaterials (ENM) have been utilized for a wide range
of applications and introduced into a number of consumer products; yet the
consequent increase in human exposure and any potential adverse effects have not
been fully evaluated. Modulation of the immune system function has been shown to be
a major toxicological consequence of ENM exposure. The implication of mast cells in
ENM-mediated toxicity, including the most widely utilized carbon and metal-based
ENMs, has been previously demonstrated; and therefore, understanding direct ENM
interaction with mast cells at the cellular and molecular level is of critical
importance for the safe implementation of ENMs into consumer products. © Springer
Science+Business Media, LLC, part of Springer Nature 2019.
AN - rayyan-553780098
AU - Alsaleh, N. B.
AU - Brown, J. M.
DO - 10.1007/978-1-4939-8916-4_2
KW - Degranulation
Engineered nanomaterials
Immunotoxicity
Mast cell
Nanoparticles
Nanotoxicity
Animals
Carbon
Cell Degranulation
Cell Survival
Cells, Cultured
Consumer Product Safety
Humans
Hypersensitivity
Mast Cells
Metals
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Models, Animal
Nanostructures
Primary Cell Culture
Toxicity Tests
beta n acetylhexosaminidase
carbon
chemokine
cytokine
Fc receptor
histamine
lysosome associated membrane protein 2
proteinase
silver nanoparticle
stem cell factor receptor
metal
nanomaterial
animal cell
animal experiment
animal model
cell maturation
cell viability
controlled study
cytokine release
human
immune system
immunocompetent cell
mast cell
mast cell degranulation
mouse
nanotoxicology
nonhuman
passive skin anaphylaxis
protein expression
animal
Bagg albino mouse
C57BL mouse
cell culture
cell survival
degranulation
devices
hypersensitivity
immunology
primary cell culture
procedures
product safety
toxicity testing
PY - 2019
SP - 31-45
ST - Methods for assessing mast cell responses to engineered nanomaterial exposure
T2 - Methods in Molecular Biology
TI - Methods for assessing mast cell responses to engineered nanomaterial exposure
85058732936&doi=10.1007%2f978-1-4939-8916-
4_2&partnerID=40&md5=d56c02295a069d59d07a6b132062d392
VL - 1894
ID - 8134
ER -
TY - JOUR
AB - Three biodegradable wound dressing based on binary Collagen (COL), Hyaluronic
acid (HA) crosslinked loaded with silver nanoparticles (AgNPs), Gentamicin (GENT)
and AgNPs/GENT successfully prepared using freeze drying technique. Chemical
evaluations for synthesized membranes were carried out using FTIR- ATR. While
physical properties were evaluated through swelling and degradation percent.
Antibacterial activity was evaluated against G+, G-, yeast and fungi. Finally,
cytotoxicity and wound healing evaluations were carried out against skin fibroblast
normal cell line, while anti-inflammatory evaluated using RAW 264.7 macrophage cell
line. The three produced membrane showed physically interaction between polymer
network and the loaded antibiotic. Swelling properties showed superior results for
three membranes. Degradability of prepared sheets was rapidly no more than three
days. Toxicity evaluations and anti-inflammatory showed superior results for all
examined samples except mixed with AgNPs and Gentamicin (GENT). Antibacterial
activity showed resistance to G+, G- and yeast. All prepared sheet showed safe
towards cell except COL/HA/AgNPs/GENT. Wound healing studied showed efficient of
both COL/HA/AgNPs and COL/HA/GENT compared to blank and mixed membrane
COL/HA/AgNPs/GENT. The obtained results recommended COL/HA loaded individually
either AgNPs or Gentamicin (GENT) as antibacterial and wound healing sheet rather
than mixed prepared membrane. © 2023 Elsevier B.V.
AN - rayyan-553780100
AU - AlSalem, H. S.
AU - Bukhari, A. A. H.
DO - 10.1016/j.ijbiomac.2023.124700
KW - Collagen
Gentamicin
Hyaluronic acid
Wound healing
Anti-Bacterial Agents
Bandages
Gentamicins
Hyaluronic Acid
Metal Nanoparticles
Saccharomyces cerevisiae
Silver
Wound Healing
Cell culture
Crosslinking
Membranes
Organic acids
Yeast
antibiotic agent
antiinfective agent
collagen
gentamicin
hyaluronic acid
nitric oxide
silver nanoparticle
metal nanoparticle
silver
Anti-bacterial activity
Anti-inflammatories
Chemical evaluation
Crosslinked
Drying technique
Freeze drying
Wound dressings
Wound healing applications
Article
attenuated total reflectance Fourier transform infrared spectroscopy
biodegradability
clinical article
cytotoxicity
fibroblast
freeze drying
human
human cell
macrophage cell line
particle size
RAW 264.7 cell line
skin fibroblast
swelling
wound healing
wound healing assay
chemistry
metabolism
PY - 2023
ST - Biodegradable wound dressing-based collagen/hyaluronic acid loaded
antibacterial agents for wound healing application
T2 - International Journal of Biological Macromolecules
TI - Biodegradable wound dressing-based collagen/hyaluronic acid loaded
antibacterial agents for wound healing application
85159578926&doi=10.1016%2fj.ijbiomac.2023.124700&partnerID=40&md5=c235efe2976d37c58
5739a6ea21335fd
VL - 242
ID - 8136
ER -
TY - JOUR
AB - Six new Ag(I) coordination complexes, namely [Ag4(μ-dicl)4(2-pic)3] 1,
[Ag2(μ-mef)2(2-pic)2] 2, [Ag2(μ-dicl)2(3-pic)2] 3, [Ag2(μ-mef)2(3-pic)2] 4, [Ag2(μ-
dicl)2(4-pic)2] 5 and [Ag2(μ-mef)2(4-pic)2] 6, were synthesized and characterized
by elemental analysis, FT-IR and thermal analysis techniques. The crystal
structures of 1, 3, 4 and 5 were determined by X-ray diffraction analysis, whereas
2 and 6 were obtained as microcrystalline powders. X-ray diffraction analysis
demonstrated that 1 occurs as a tetranuclear complex, while 3, 4 and 5 have
binuclear structures. A short Ag⋯Ag distances of 2.8446 and 2.8823 Å for 1, 2.8409
Å for 3, 2.8942 Å for 4 and 2.8423 Å for 5 were found in the complexes, indicating
that argentophilic interactions exist between the silver ions. In 1, the dicl
ligands act as μ3-O,O′,O′ bridging ligands between three Ag(I) ions. In 3, 4 and 5,
the dicl and mef ligands behave as bridging ligands and bind two Ag(I) ions
together with the carboxylato oxygen atoms. The simultaneous TG/DTG and DTA
techniques were applied to interpret the mass losses, decomposition temperatures
and corresponding processes of the thermal behaviours of the complexes. The
experimental and calculated mass losses of the proposed structures for complexes 2
and 6 are quite compatible with each other and these complexes have very similar
FT-IR spectra to the other complexes, which reflect their same structural
geometries and characteristics. The cytotoxic activities of the complexes (1–6)
were tested against three different cancer cell lines, MCF-7, HT-29 and HepG2, and
one normal cell line, 3 T3-L1. XTT assay results demonstrated that although all the
silver(I) complexes showed good cytotoxic activity, depending on the cell types
tested, the complexes with mefanamic acid (2, 4 and 6) were found to predominate
over those with diclofenac (1, 3 and 5), as well as the superiority of 3-picoline
to 4-picoline and 2-picoline. Moreover, compared with the cytotoxic potential of
carboplatin, 4 especially exhibited a significant cancer cell inhibitory rate and
lower cytotoxicity toward the normal cell line, with much higher selectivity
indexes. © 2018 Elsevier Ltd
AN - rayyan-553780103
AU - Altay, A.
AU - Caglar, S.
AU - Caglar, B.
AU - Sahin, O.
DO - 10.1016/j.poly.2018.05.038
KW - Cell culture
Cytotoxicity
Diclofenac
Mefenamic acid
Silver(I) complexes
Mefenamic Acid
PY - 2018
SP - 160-170
ST - Synthesis, structural, thermal elucidation and in vitro anticancer activity
of novel silver(I) complexes with non-steroidal anti-inflammatory drugs diclofenac
and mefenamic acid including picoline derivatives
T2 - Polyhedron
TI - Synthesis, structural, thermal elucidation and in vitro anticancer activity
of novel silver(I) complexes with non-steroidal anti-inflammatory drugs diclofenac
and mefenamic acid including picoline derivatives
85047825401&doi=10.1016%2fj.poly.2018.05.038&partnerID=40&md5=56aa82bea48332fceb009
bebacfa4e2e
VL - 151
ID - 8139
ER -
TY - JOUR
AB - The treatment of wounds is expensive and challenging. Most of the available
wound dressings are not effective and suffer from limitations such as poor
antimicrobial activity, toxicity, inability to provide suitable moisture to the
wound and poor mechanical performance. The use of inappropriate wound dressings can
result in a delayed wound healing process. Nanosize range scaffolds have triggered
great attention because of their attractive properties, which include their
capability to deliver bioactive agents, high surface area, improved mechanical
properties, mimic the extracellular matrix (ECM), and high porosity. Nanofibrous
materials can be further encap-sulated/loaded with metal-based nanoparticles to
enhance their therapeutic outcomes in wound healing applications. The widely
studied metal-based nanoparticles, silver nanoparticles exhibit good properties
such as outstanding antibacterial activity, display antioxidant, and anti-
inflammatory properties, support cell growth, making it an essential bioactive
agent in wound dressings. This review article reports the biological (in vivo and
in vitro) and mechanical outcomes of nanofibrous scaffolds loaded with silver
nanoparticles on wound healing. © 2021 by the authors. Licensee MDPI, Basel,
Switzerland.
AN - rayyan-553780106
AU - Alven, S.
AU - Buyana, B.
AU - Feketshane, Z.
AU - Aderibigbe, B. A.
DO - 10.3390/pharmaceutics13070964
IS - 7
KW - Nanofibers
Nanofibrous mats
Nanofibrous membranes
Nanoparticles
Scaffolds
Wound dressings
Wound treatment
cellulose acetate
chitosan
electrospun nanofiber
gum arabic
macrogol
molecular scaffold
nanofiber
nanomaterial
polycaprolactone
polyethylene
polyglactin
polylactic acid
polyvinyl alcohol
silver nanoparticle
unclassified drug
cell growth
electrospinning
extracellular matrix
human
in vitro study
mechanical stimulation
nanoencapsulation
nonhuman
porosity
Review
surface area
therapy delay
toxicity testing
treatment outcome
wound care
wound healing
Bandages
PY - 2021
ST - Electrospun nanofibers/nanofibrous scaffolds loaded with silver nanoparticles
as effective antibacterial wound dressing materials
T2 - Pharmaceutics
TI - Electrospun nanofibers/nanofibrous scaffolds loaded with silver nanoparticles
as effective antibacterial wound dressing materials
85109390469&doi=10.3390%2fpharmaceutics13070964&partnerID=40&md5=2eb7af684bbeb9c9cc
45fbf30a17061f
VL - 13
ID - 8142
ER -
TY - JOUR
AB - Silver has been used in medical application for its antibacterial,
antifungal, and anti-inflammatory effects. Silver nanoparticles (AgNPs) are
currently in the spotlight. It was shown that their application can be useful in
the management of wounds. Our study was conducted to determine whether AgNPs
(average size 10.43 +/- 4.74 nm) and ionic silver (Ag-I) could affect the wound
healing in the in vitro model of normal human dermal fibroblasts (NHDF). We
evaluated their effect on reactive oxygen species (ROS) generation and the
expression of key transcription factors that coordinate the cellular response to
oxidative stress [nuclear factor (erythroid-derived 2)-like 2 (Nrf2)] and
inflammation [nuclear factor-kappa B (NF-kappa B)], expression of heme oxygenase-1
(HO-1), and interleukin-6 (IL-6) level. Isolated primary NHDF were scratched,
heated (1 h; 42 degrees C), and cultured with AgNPs (0.25, 2.5, and 25 mu g/ml) and
Ag-I (0.025, 0.1, and 0.25 mu g/ml) for 8 or 24 h. The ROS generation, Nrf2, NF-
kappa B, and HO-1 protein expression and IL-6 protein level were then evaluated by
standard methods. Non-cytotoxic concentrations of AgNPs (0.25 and 2.5 mu g/ml) did
not affect the ROS generation but activated the Nrf2/ HO-1 pathway and decreased
the NF-.B expression and IL-6 level in the in vitro wound healing model. AgNPs at
concentrations of 0.25 and 2.5 mu g/ml seem to be suitable for the intended
application as a topical agent for wound healing, although the gene silencing
technique, chemical inhibitors, and detailed time-and concentration-dependent
experiments are needed for a comprehensive study of signaling pathway regulation.
Further investigation is also necessary to exclude any possible adverse effects.
AN - rayyan-553780108
AU - Ambrozova, N.
AU - Zalesak, B.
AU - Ulrichova, J.
AU - Cizkova, K.
AU - Galandakova, A.
DO - 10.1007/s11051-017-3809-7
IS - 3
KW - Wound Healing
PY - 2017
SN - 1388-0764 1572-896X
ST - Low concentrations of silver nanoparticles have a beneficial effect on wound
healing in vitro
T2 - JOURNAL OF NANOPARTICLE RESEARCH
TI - Low concentrations of silver nanoparticles have a beneficial effect on wound
healing in vitro
VL - 19
Y2 - 3 y3 - 13
ID - 8144
ER -
TY - JOUR
AB - Cyclosporine is a calcineurin inhibitor that acts selectively on T cells. It
has been used in dermatology since 1997 for its US Food and Drug Administration
indication of psoriasis and off-label for various other inflammatory skin
conditions, including atopic dermatitis, blistering disorders, and connective
tissue diseases. In the last decade, many dermatologists have hesitated to use this
important drug in their clinical practices because of its toxicity profile. The
purpose of this article is to review the mechanism of action of cyclosporine and
its current uses and dosing schedules. It is our goal to create a framework in
which dermatologists feel comfortable and safe incorporating cyclosporine into
their prescribing regimens. Learning objectives: After completing this learning
activity, participants should be able to describe the mechanism of action of
cyclosporine, recognize the potential role of cyclosporine in dermatology and the
evidence to support this role, and incorporate cyclosporine into his or her
prescribing regimens. © 2010 by the American Academy of Dermatology, Inc.
AN - rayyan-553780112
AU - Amor, K. T.
AU - Ryan, C.
AU - Menter, A.
DO - 10.1016/j.jaad.2010.02.063
IS - 6
KW - Atopic dermatitis
Chronic urticaria
Cyclosporine
Psoriasis
Pyoderma gangrenosum
Dermatologic Agents
Dermatology
Education, Medical, Continuing
Humans
Skin Diseases
alpha interferon
azathioprine
betamethasone dipropionate
betamethasone valerate
capsaicin
cetirizine
colchicine
colecalciferol derivative
corticosteroid
cyclophosphamide
cyclosporin
cyclosporin A
dapsone
etretin
fumaric acid
gold
itraconazole
methotrexate
methylprednisolone
mycophenolic acid 2 morpholinoethyl ester
nonsteroid antiinflammatory agent
prednisolone
prednisone
rituximab
salazosulfapyridine
sulfadiazine silver
tacrolimus
triamcinolone acetonide
tumor necrosis factor inhibitor
unindexed drug
absence of side effects
acrodermatitis continua
alopecia
alopecia areata
alopecia totalis
aphthous ulcer
ascites
atopic dermatitis
Behcet disease
chronic urticaria
clinical trial
competitive inhibition
corticosteroid therapy
creatine kinase blood level
cryotherapy
dermatomyositis
diarrhea
dose response
drug dose increase
drug dose reduction
drug dose regimen
drug efficacy
drug intermittent therapy
drug mechanism
drug megadose
drug pulse therapy
drug structure
drug toxicity
drug treatment failure
edema
eospinophilic pustular folliculitis
epidermolysis bullosa acquisita
erythrodermic psoriasis
folliculitis
genital ulcer
graft rejection
Hailey Hailey disease
hirsutism
human
hypertension
immunosuppressive treatment
lichen planus
low drug dose
microemulsion
monotherapy
mouth ulcer
nephrotoxicity
pancreatitis
papular skin disease
pemphigus vulgaris
peripheral neuropathy
photodermatosis
pityriasis rubra pilaris
pompholyx
prescription
priority journal
protein phosphorylation
prurigo nodularis
psoriasis
psoriatic arthritis
pustulosis palmoplantaris
pyoderma gangrenosum
quality of life
review
Schnitzler syndrome
scleroderma
side effect
skin inflammation
solar urticaria
split thickness skin graft
suppurative hidradenitis
tachyphylaxis
therapy effect
treatment duration
ultraviolet A radiation
ultraviolet B radiation
unspecified side effect
urticaria
Cyclosporins
PY - 2010
SP - 925-946
ST - The use of cyclosporine in dermatology: Part i
T2 - Journal of the American Academy of Dermatology
TI - The use of cyclosporine in dermatology: Part i
78649387833&doi=10.1016%2fj.jaad.2010.02.063&partnerID=40&md5=9f7af53d4ec158cb99928
8a62d8f45c0
VL - 63
ID - 8148
ER -
TY - JOUR
AB - The NF-kappa B family of transcription factors plays an important role in
determining cell survival during immune, inflammatory, and stress responses. NF-
kappa B activity is frequently deregulated in human cancers and is implicated in
the resistance of tumor cells to diverse anticancer agents. We studied the effects
of novel analogs of precursors of the natural product simplactone (A) on the
activity of IkB kinase and NF-kappa B. Screening of six compounds for the ability
to inhibit TNF-induced NF-kappa B activity revealed that compound SK2009 was the
most potent of these compounds in suppressing NF-kappa B activation in KBM-5
leukemic cells. Further characterization of SK2009 indicates that this newly
synthesized molecule can suppress TNF-induced I kappa B alpha kinase activation and
inhibit the expression of three NF-kappa B-dependent gene products, cyclin D1, Bcl-
2, and VEGF, in these cells. Published by Elsevier Ltd.
AN - rayyan-553780113
AU - Anchoori, R. K.
AU - Harikumar, K. B.
AU - Batchu, V. R.
AU - Aggarwal, B. B.
AU - Khan, S. R.
DO - 10.1016/j.bmc.2009.10.065
IS - 1
KW - NF-kappa B
PY - 2010
SN - 0968-0896 1464-3391
SP - 229-235
ST - Inhibition of IkB kinase and NF-kappa B by a novel synthetic compound SK 2009
T2 - BIOORGANIC & MEDICINAL CHEMISTRY
TI - Inhibition of IkB kinase and NF-kappa B by a novel synthetic compound SK 2009
VL - 18
Y2 - 1 y3 - 1
ID - 8149
ER -
TY - JOUR
AB - The review highlights the need of non-antibacterial, non-antifungal and non-
anticancer characters of metal or metal oxide nanoparticles. The usage of
nanoparticles as a part of therapeutic measures results in certain unfavourable
effects. The nanoparticles can disturb healthy gut microorganisms that may bring
about some health damages regarding pathogenic diseases, obesity, and inflammation
likewise. Even the nonspecific interactions of nanoparticles with healthy cells and
tissues can cause altered expressions of various pro-inflammatory factors and
stress related genes. This review indicates and prospect about the demand of
nanoparticles with non-antibacterial, non-antifungal and non-anticancer properties.
Such nanoparticles will be effective in various remedial and diagnostic purposes.
AN - rayyan-553780114
AU - Ankamwar, B.
AU - Yadwade, R.
DO - 10.1088/2632-959X/abe473
IS - 1
PY - 2021
SN - 2632-959X
ST - A review: non-antibacterial, non-antifungal and non-anticancer properties of
nanoparticles the forgotten paradigm
T2 - NANO EXPRESS
TI - A review: non-antibacterial, non-antifungal and non-anticancer properties of
nanoparticles the forgotten paradigm
VL - 2
Y2 - 3 y3 - 1
ID - 8150
ER -
TY - JOUR
AB - Nowadays, bone diseases and defects as a result of trauma, cancers,
infections and degenerative and inflammatory conditions are increasing.
Consequently, bone repair and replacement have been developed with improvement of
orthopedic technologies and biomaterials of superior properties. This review paper
is intended to sum up and discuss the most relevant studies performed in the field
of bone biology and bone regeneration approaches. Therefore, the bone tissue
regeneration was investigated by synthetic substitutes, scaffolds incorporating
active molecules, nanomedicine, cell-based products, biomimetic fibrous and
nonfibrous substitutes, biomaterial-based three-dimensional (3D) cell-printing
substitutes, bioactive porous polymer/inorganic composites, magnetic field and
nano-scaffolds with stem cells and bone–biomaterials interface studies. © 2019, The
Author(s).
AN - rayyan-553780116
AU - Ansari, M.
DO - 10.1007/s40204-019-00125-z
IS - 4
KW - Biocompatibility
Biology
Biomaterials
Bone regeneration
Tissue engineering
3 hydroxybutyric acid
antibiotic agent
bioceramics
biomaterial
bone morphogenetic protein 2
bone morphogenetic protein receptor
calcium ion
calcium phosphate
collagen type 1
growth differentiation factor 5
hydroxyapatite
hydroxyprogesterone caproate
magnetic nanoparticle
matrix protein
osteogenic protein 1
osteogenin
osteopontin
parathyroid hormone
poly(methyl methacrylate)
polycaprolactone
polyetheretherketone
polyglactin
polyglycolic acid
polyhydroxyalkanoic acid
protein p15
selenium nanoparticle
silicate
silver nanoparticle
transforming growth factor beta1
unindexed drug
angiogenesis
biodegradability
biology
bone cell
bone development
bone disease
bone injury
bone matrix
bone regeneration
bone remodeling
bone structure
bone tissue
circulation
collagen fiber
computer assisted tomography
degeneration
dental pulp stem cell
electromagnetism
human
inflammation
mesenchymal stem cell
nanomedicine
nonhuman
orthopedics
osteocyte
priority journal
Review
static electricity
three-dimensional imaging
tissue engineering
tissue regeneration
Regeneration
PY - 2019
SP - 223-237
ST - Bone tissue regeneration: biology, strategies and interface studies
T2 - Progress in Biomaterials
TI - Bone tissue regeneration: biology, strategies and interface studies
85126715689&doi=10.1007%2fs40204-019-00125-
z&partnerID=40&md5=d40f733c1f3394b2d6dda80048614495
VL - 8
ID - 8152
ER -
TY - JOUR
AB - Wounds represent a major healthcare problem especially in hospital-associated
infections where multi-drug resistant strains are often involved. Nowadays,
biomaterials with therapeutic molecules play an active role in wound healing and
infection prevention. In this work, the development of collagen hydrogels loaded
with silver nanoparticles and Cannabis sativa oil extract is described. The
presence of the silver nanoparticles gives interesting feature to the biomaterial
such as improved mechanical properties or resistance to collagenase degradation but
most important is the long-lasting antimicrobial effect. Cannabis sativa oil, which
is known for its anti-inflammatory and analgesic effects, possesses antioxidant
activity and successfully improved the biocompatibility and also enhances the
antimicrobial activity of the nanocomposite. Altogether, these results suggest that
this novel nanocomposite biomaterial is a promising alternative to common
treatments of wound infections and wound healing. © 2021 by the authors. Licensee
AN - rayyan-553780118
AU - Antezana, P. E.
AU - Municoy, S.
AU - Pérez, C. J.
AU - Desimone, M. F.
IS - 11
KW - Antimicrobial
Biomaterials
Cannabis sativa
Collagen
Wound healing
ammonia
antiinfective agent
antioxidant
biomaterial
cannabidiol
cannabigerol
cannabis sativa oil
collagen
collagen gel
collagen type 1
collagenase
dronabinol
essential oil
fibrillar collagen
hydrogel
hydroxyproline
metalloproteinase
nanocomposite
nanoparticle
silver nanoparticle
sodium borohydride
unclassified drug
analgesic activity
Article
atomic absorption spectrometry
bacterial growth
biocompatibility
biodegradability
carcinogenicity
cell proliferation
cell viability
colloid
colorimetry
controlled study
crystallization
cytotoxicity
disk diffusion
Fourier transform infrared spectroscopy
Gram negative bacterium
Gram positive bacterium
hospital infection
isotherm
MTT assay
nonhuman
oxidative stress
particle size
surface plasmon resonance
surface property
ultraviolet visible spectroscopy
viscosity
wound healing
wound infection
zone of inhibition
Cannabis
PY - 2021
ST - Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil
T2 - Antibiotics
TI - Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil
85122376182&doi=10.3390%2fantibiotics10111420&partnerID=40&md5=b0d5e8c76567e9152bdb
be5701185100
VL - 10
ID - 8154
ER -
TY - JOUR
AB - The present study was aimed to synthesize silver nanoparticles (AgNPs) from
the aqueous extracts of Odontosoria chinensis (L.) J. Sm. and the synthesized AgNPs
were examined for their biopotentials. The Odontosoria chinensis extracts were
added to 1 mM AgNO3 solution with different ratios viz., 0.5: 9.5, 1:9, 1.5: 8.5
and 2: 8 ratios for the reduction of Ag ions. After reduction, the AgNPs of
Odontosoria chinensis were analyzed spectroscopically for further confirmation. The
synthesized AgNPs of Odontosoria chinensis were characterized by pH, ultra violet–
visible spectroscopy (UV-Vis), Fourier transform–infra red spectroscopy (FT-IR),
scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDAX) and X-Ray
diffraction (XRD). The time taken for the complete reduction of Silver (Ag) in
solution to nanoparticle was 10 min. The O. chinensis aqueous extracts mediated
silver nanoparticles showed a broad peak with distinct absorption at around 400–420
nm and confirmed the silver nanoparticle formation. FT-IR results also confirmed
the existence of organic materials in the silver nanoparticles of O. chinensis. The
EDX spectra of AgNPs of O. chinenesis revealed the occurrence of a strong Ag peak.
The synthesis of AgNPs of O. chinenesis was confirmed with the existence of a peak
at 46.228°. The toxic potential of AgNPs of O. chinenesis showed varied percentage
mortality with the LC50 values of 134.68 μL/ 50 mL and 76.5 μL/50 mL, respectively.
The anti-inflammatory and anti-diabetic activities of aqueous and AgNPs of O.
chinenesis were statistically significant at p < 0.05 level. Conclusion: The
results demonstrated the toxicity, anti-diabetic and anti-inflammatory potential of
the studied AgNPs. The synthesized nanoparticles of Odontosoria chinensis could be
tested as an alternative to anticancer, anti-diabetic and anti-inflammatory drugs.
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780119
AU - Antonysamy Johnson, M. A.
AU - Shibila, T.
AU - Amutha, S.
AU - Menezes, I. R. A.
AU - Da Costa, J. G. M.
AU - Sampaio, N. F. L.
AU - Coutinho, H. D. M.
DO - 10.3390/ph13040066
IS - 4
KW - Anti-diabetic
Anti-inflammatory
Cytotoxic
Odontosoria chinenesis
amylase
Odontosoria chinensis extract
plant extract
silver nanoparticle
silver nitrate
unclassified drug
antidiabetic activity
aqueous solution
Article
cell suspension
controlled study
energy dispersive X ray spectroscopy
enzyme inhibition
erythrocyte
fern
hemolysis
human
human cell
human experiment
LC50
mortality
normal human
Odontosoria chinensis
pH
synthesis
time
volunteer
X ray diffraction
PY - 2020
ST - Synthesis of silver nanoparticles using odontosoria chinensis (L.) J. sm. and
evaluation of their biological potentials
T2 - Pharmaceuticals
TI - Synthesis of silver nanoparticles using odontosoria chinensis (L.) J. sm. and
evaluation of their biological potentials
85084145336&doi=10.3390%2fph13040066&partnerID=40&md5=db282cc35f1ca868226da1f641e5d
658
VL - 13
ID - 8155
ER -
TY - JOUR
AB - The increase in the usage of silica nanoparticles (SiNPs) in the industrial
and medical fields has raised concerns about their possible adverse effects on
human health. The present study aimed to investigate the potential adverse effects
of SiNPs at daily doses of 25 and 100 mg/kg body weight intraperitoneally (i.p.)
for 28 consecutive days on markers of liver damage in adult male rats. Results
revealed that SiNPs induced a marked increase in serum markers of liver damage,
including lactate dehydrogenase (LDH), alanine aminotransferase (ALAT), and
aspartate aminotransferase (ASAT). SiNPs also induced an elevation of reactive
oxygen species (ROS) production in liver, along with an increase in oxidative
stress markers (NO, MDA, PCO, and H2O2), and a decrease in antioxidant enzyme
activities (CAT, SOD, and GPx). Quantitative real-time PCR showed that SiNPs also
induced upregulation of pro-apoptotic gene expression (including Bax, p53, Caspase-
9/3) and downregulation of anti-apoptotic factors Bcl-2. Moreover,
histopathological analysis revealed that SiNPs induced hepatocyte alterations,
which was accompanied by sinusoidal dilatation, Kupffer cell hyperplasia, and the
presence of inflammatory cells in the liver. Taken together, these data showed that
SiNPs trigger hepatic damage through ROS-activated caspase signaling pathway, which
plays a fundamental role in SiNP-induced apoptosis in the liver.
AN - rayyan-553780120
AU - Aouey, B.
AU - Boukholda, K.
AU - Gargouri, B.
AU - Bhatia, H. S.
AU - Attaai, A.
AU - Kebieche, M.
AU - Bouchard, M.
AU - Fetoui, H.
DO - 10.1007/s12011-021-02774-3
IS - 4
KW - Apoptosis
Signal Transduction
PY - 2022
SN - 0163-4984 1559-0720
SP - 1688-1698
ST - Silica Nanoparticles Induce Hepatotoxicity by Triggering Oxidative Damage,
Apoptosis, and Bax-Bcl2 Signaling Pathway
T2 - BIOLOGICAL TRACE ELEMENT RESEARCH
TI - Silica Nanoparticles Induce Hepatotoxicity by Triggering Oxidative Damage,
Apoptosis, and Bax-Bcl2 Signaling Pathway
VL - 200
Y2 - 4
ID - 8156
ER -
TY - JOUR
AB - Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different
industrial and biomedical fields. Several studies have reported that NiO NPs may
affect the development of reproductive organs inducing oxidative stress and,
resulting in male infertility. We investigated the in vitro effects of NiO NPs on
porcine pre-pubertal Sertoli cells (SCs) which undergone acute (24 h) and chronic
(from 1 up to 3 weeks) exposure at two subtoxic doses of NiO NPs of 1 mu g/ml and 5
mu g/ml. After NiO NPs exposure we performed the following analysis: (a) SCs
morphological analysis (Light Microscopy); (b) ROS production and oxidative DNA
damage, gene expression of antioxidant enzymes (c) SCs functionality (AMH, inhibin
B Real-time PCR analysis and ELISA test); (d) apoptosis (WB analysis); (e) pro-
inflammatory cytokines (Real-time PCR analysis), and (f) MAPK kinase signaling
pathway (WB analysis). We found that the SCs exposed to both subtoxic doses of NiO
NPs didn't sustain substantial morphological changes. NiO NPs exposure, at each
concentration, reported a marked increase of intracellular ROS at the third week of
treatment and DNA damage at all exposure times. We demonstrated, un up-regulation
of SOD and HO-1 gene expression, at both concentrations tested. The both subtoxic
doses of NiO NPs detected a down-regulation of AMH and inhibin B gene expression
and secreted proteins. Only the 5 mu g/ml dose induced the activation of caspase-3
at the third week. At the two subtoxic doses of NiO NPs a clear pro-inflammatory
response was resulted in an up-regulation of TNF-alpha and IL-6 in terms of mRNA.
Finally, an increased phosphorylation ratio of p-ERK1/2, p-38 and p-AKT was
observed up to the third week, at both concentrations. Our results show the
negative impact of subtoxic doses NiO NPs chronic exposure on porcine SCs
functionality and viability.
AN - rayyan-553780124
AU - Arato, I.
AU - Giovagnoli, S.
AU - Di Michele, A.
AU - Bellucci, C.
AU - Lilli, C.
AU - Aglietti, M. C.
AU - Bartolini, D.
AU - Gambelunghe, A.
AU - Muzi, G.
AU - Calvitti, M.
AU - Eugeni, E.
AU - Gaggia, F.
AU - Baroni, T.
AU - Mancuso, F.
AU - Luca, G.
DO - 10.3389/fendo.2023.1063916
KW - Nickel
Infertility, Male
PY - 2023
SN - 1664-2392
ST - Nickel oxide nanoparticles exposure as a risk factor for male infertility:
"In vitro" effects on porcine pre-pubertal Sertoli cells
T2 - FRONTIERS IN ENDOCRINOLOGY
TI - Nickel oxide nanoparticles exposure as a risk factor for male infertility:
"In vitro" effects on porcine pre-pubertal Sertoli cells
VL - 14
Y2 - 3 y3 - 30
ID - 8160
ER -
TY - JOUR
AB - Introducción: Los fibroblastos gingivales humanos (FGH) tienen un papel
importante en la enfermedad periodontal, pues alteran su normal funcionamiento en
respuesta a estímulos pro-inflamatorios. Se cree que los fibroblastos se pueden
eliminar anormalmente por medio de apoptosis en periodontitis. El propósito de este
estudio es determinar y cuantificar la apoptosis de FGH en biopsias del periodonto
de individuos sanos y con enfermedad periodontal. Métodos: Se realizó un estudio
clínico descriptivo de corte transversal en personas con diagnóstico de salud
periodontal (S), gingivitis (G) y periodontitis crónica (PC). Se tomaron biopsias
escisionales y se hicieron tinciones inmunohistoquímicas (hematoxilina-eosina,
caspasa-3 y vimentina). Las placas se interpretaron por histopatología y se
digitalizaron para cuantificar las células apoptóticas. Todos los datos se
analizaron con un software estadístico para encontrar diferencias significativas
(p<0.05). Resultados: La población celular total de fibroblastos tuvo un promedio
de 430±67.6 en los individuos sanos y una disminución significativamente progresiva
en gingivitis (270±37.1) y periodontitis crónica (206.5±69.8) (p<0.05). La
expresión de fibroblastos apoptóticos por campo aumentó de acuerdo con las
severidades de la enfermedad [28±16 en sanos (6.5%); 31±17 en gingivitis (11.5%) y
51±24 en periodontitis (24.8%.), (p<0.001)]. La relación entre la expresión de
fibroblastos apoptóticos y la profundidad de la bolsa periodontal no fue
significante (p>0.5, r²=0.02); mientras que para las células inflamatorias se
encontró una relación proporcional significativa (p<0.05, r²=0.2018). Introduction:
Human gingival fibroblasts (HGF) have an important role in the periodontal immune
response. The fibroblasts alter their normal behavior in response to pro-
inflammatory cytokines. It is believed that HGF can be diminished and/or eliminated
by means of apoptosis. The purpose of this study was to determine and to quantify
apoptosis of HGF in periodontium biopsies from healthy and chronic periodontitis
patients. Methods: A clinical cross-sectional study in people with healthy
periodontium (S), gingivitis (G) and chronic periodontitis (PC) patients was
carried out. The periodontal biopsies were obtained and immunostained by means of:
hematoxylin-eosin, caspase-3, vimentin and caspase-vimentin double-staining for
specific visualization of apoptotic fibroblasts. Histopathological and digital
analyses were performed. Descriptive statistics were applied to categorical and
nominal variables. Results: Total cell population of HGF had an average of 430±67.6
cells/field in healthy people, and a significantly progressive decrease in
gingivitis (270±37.1) and chronic periodontitis groups (206.5±69.8) (p<0.05). As
for total population of inflammatory cells, an increase was noticed in gingivitis
(191.8±50.1) and a decrease in periodontitis (109.3±21.7) without statistical
significance. The expression of apoptotic HGF per field increased accordingly to
the severity of the disease [28±16 in health (6.5%); 31±17 in gingivitis (11.5%)
and 51±24 in periodontitis (24.8%), p<0.001]. Similar findings were observed for
inflammatory cells with different percentage expression [17±13 in health (23%);
28±19 in gingivitis (14.6%) and 47±35 in periodontitis (43.1%), p<0.05]. The
relationship between the percentage of expression of apoptotic cells and probing
pocket depth was proportional but not significant (p>0.5, r²=0.02); while for the
inflammatory cells a significant relationship was observed (p<0.05, r²=0.2018).
AN - rayyan-553780125
AU - Arce, Roger Mauricio
AU - Tamayo, Oscar
AU - Cortés, Armando
IS - 3
KW - Apoptosis
Fibroblasts
Gingival
Gingivitis
Immunohistochemistry
Inmunohistoquímica
Pathogenesis
Patogénesis
Periodontitis
Periodontics
LA - es
PY - 2007
SN - 0120-8322
SP - 197-209
ST - Apoptosis de fibroblastos gingivales en periodontitis
T2 - Colomb. med
TI - Apoptosis de fibroblastos gingivales en periodontitis
UR - http://colombiamedica.univalle.edu.co/index.php/comedica/article/view/505/984
VL - 38
Y2 - 9
ID - 8161
ER -
TY - JOUR
AB - Bacterial nanocellulose (BNC) is a novel nanomaterial known for its large
surface area, biocompatibility, and non-toxicity. BNC contributes to regenerative
processes in the skin but lacks antimicrobial and anti-inflammatory properties.
Herein, the development of bioactive wound dressings by loading antibacterial
povidone-iodine (PVI) or anti-inflammatory acetylsalicylic acid (ASA) into
bacterial cellulose is presented. BNC is produced using Hestrin-Schramm culture
media and loaded via immersion in PVI and ASA. Through scanning electron
microscopy, BNC reveals open porosity where the bioactive compounds are loaded; the
mechanical tests show that the dressing prevents mechanical wear. The loading
kinetic and release assays (using the Franz cell method) under simulated fluids
present a maximum loading of 589.36 mg PVI/g BNC and 38.61 mg ASA/g BNC, and both
systems present a slow release profile at 24 h. Through histology, the complete
diffusion of the bioactive compounds is observed across the layers of porcine skin.
Finally, in the antimicrobial experiment, BNC/PVI produced an inhibition halo for
Gram-positive and Gram-negative bacteria, confirming the antibacterial activity.
Meanwhile, the protein denaturation test shows effective anti-inflammatory activity
in BNC/ASA dressings. Accordingly, BNC is a suitable platform for the development
of bioactive wound dressings, particularly those with antibacterial and anti-
inflammatory properties.
AN - rayyan-553780126
AU - Argel, S.
AU - Castano, M.
AU - Jimenez, D. E.
AU - Rodriguez, S.
AU - Vallejo, M. J.
AU - Castro, C. I.
AU - Osorio, M. A.
IS - 8
PY - 2022
SN - 1999-4923
ST - Assessment of Bacterial Nanocellulose Loaded with Acetylsalicylic Acid or
Povidone-Iodine as Bioactive Dressings for Skin and Soft Tissue Infections
T2 - PHARMACEUTICS
TI - Assessment of Bacterial Nanocellulose Loaded with Acetylsalicylic Acid or
Povidone-Iodine as Bioactive Dressings for Skin and Soft Tissue Infections
VL - 14
Y2 - 8
ID - 8162
ER -
TY - JOUR
AB - The aim of the present review article was to analyze the biological impact
and toxicology of titanium dioxide nanoparticles (TiO2 NPs) to determine their
cytotoxicity, reactive oxygen species production as well as induction of oxidative
stress through a systematic review of the literature published so far about the
biocompatibility of TiO2 NPs in contact with oral cells. Available data on
nanoparticles (NPs) were collected from the PubMed and Science Direct electronic
databases, and other sources according to PRISMA recommendations for systematic
reviews. In the qualitative analysis of published data on cytotoxicity in oral
cells, a slight increase in the number of metabolically active cells has been
observed when the TiO2 NPs are in contact with oral cells; however, at certain
doses cellular viability decreases significantly. One of the negative effects of
NPs is the induction of prostaglandin E-2 production in a previous pro-inflammatory
state, which could increase the existing inflammatory effect. Therefore, the
biological application of these nanoparticles must be performed both in vivo and
metabolomic studies, since it is possible that the results of the in vitro
experiments cannot be extrapolated to an in vivo system, since it been observed
that the particles tend to aggregated among them, in the culture medium, used for
such as tests. Therefore, although it can be considered a material with light or
even biocompatible cytotoxicity, it must not be considered completely harmless.
AN - rayyan-553780127
AU - Argueta-Figueroa, L.
AU - Torres-Gomez, N.
AU - Scougall-Vilchis, R. J.
AU - Garcia-Contreras, R.
DO - 10.22209/IC.v59n4a06
IS - 4
KW - Titanium
PY - 2018
SN - 0535-5133
SP - 352-368
ST - Biocompatibility and nanotoxicology of titanium dioxide in the oral cavity:
Systematic review
T2 - INVESTIGACION CLINICA
TI - Biocompatibility and nanotoxicology of titanium dioxide in the oral cavity:
Systematic review
VL - 59
ID - 8163
ER -
TY - JOUR
AB - Stroke is among the three leading causes of death worldwide and the most
frequent cause of permanent disability. Brain ischemia induces an inflammatory
response involving activated complement fragments. Here we show that i.v. Ig (IVIG)
treatment, which scavenges complement fragments, protects brain cells against the
deleterious effects of experimental ischemia and reperfusion (I/R) and prevents
I/R-induced mortality in mice. Animals administered IVIG either 30 min before
ischemia or after 3 h of reperfusion exhibited a 50-60% reduction of brain infarct
size and a 2- to 3-fold improvement of the functional outcome. Even a single low
dose of IVIG given after stroke was effective. IVIG was protective in the
nonreperfusion model of murine stroke as well and did not exert any peripheral
effects. Human IgG as well as intrinsic murine C3 levels were significantly higher
in the infarcted brain region compared with the noninjured side, and their physical
association was demonstrated by immuno-coprecipitation. C5-deficient mice were
significantly protected from I/R injury compared with their wild-type littermates.
Exposure of cultured neurons to oxygen/glucose deprivation resulted in increased
levels of C3 associated with activation of caspase 3, a marker of apoptosis; both
signals were attenuated with IVIG treatment. Our data suggest a major role for
complement-mediated cell death in ischemic brain injury and the prospect of using
IVIG in relatively low doses as an interventional therapy for stroke.
AN - rayyan-553780129
AU - Arumugam, T. V.
AU - Tang, S. C.
AU - Lathia, J. D.
AU - Cheng, A.
AU - Mughal, M. R.
AU - Chigurupati, S.
AU - Magnus, T.
AU - Chan, S. L.
AU - Jo, D. G.
AU - Ouyang, X.
AU - Fairlie, D. P.
AU - Granger, D. N.
AU - Vortmeyer, A.
AU - Basta, M.
AU - Mattson, M. P.
DO - 10.1073/pnas.0700506104
IS - 35
KW - Immunoglobulin D
Immunoglobulin M
Immunoglobulin Variable Region
Immunoglobulins
Immunoglobulin A
Immunoglobulin E
Immunoglobulin G
Immunoglobulins, Intravenous
PY - 2007
SN - 0027-8424
SP - 14104-14109
ST - Intravenous immunoglobulin (IVIG) protects the brain against experimental
stroke by preventing complement-mediated neuronal cell death
T2 - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
TI - Intravenous immunoglobulin (IVIG) protects the brain against experimental
stroke by preventing complement-mediated neuronal cell death
VL - 104
Y2 - 8 y3 - 28
ID - 8165
ER -
TY - JOUR
AB - Exposure to blast overpressure waves is implicated as the major cause of
ocular injuries and resultant visual dysfunction in veterans involved in recent
combat operations. No effective therapeutic strategies have been developed so far
for blast-induced ocular dysfunction. Lysophosphatidic acid (LPA) is a bioactive
phospholipid generated by activated platelets, astrocytes, choroidal plexus cells,
and microglia and is reported to play major roles in stimulating inflammatory
processes. The levels of LPA in the cerebrospinal fluid have been reported to
increase acutely in patients with traumatic brain injury (TBI) as well as in a
controlled cortical impact (CCI) TBI model in mice. In the present study, we have
evaluated the efficacy of a single intravenous administration of a monoclonal LPA
antibody (25 mg/kg) given at 1 h post-blast for protection against injuries to the
retina and associated ocular dysfunctions. Our results show that a single 19 psi
blast exposure significantly increased the levels of several species of LPA in
blood plasma at 1 and 4 h post-blast. The anti-LPA antibody treatment significantly
decreased glial cell activation and preserved neuronal cell morphology in the
retina on day 8 after blast exposure. Optokinetic measurements indicated that anti-
LPA antibody treatment significantly improved visual acuity in both eyes on days 2
and 6 post-blast exposure. Anti-LPA antibody treatment significantly increased rod
photoreceptor and bipolar neuronal cell signaling in both eyes on day 7 post-blast
exposure. These results suggest that blast exposure triggers release of LPAs, which
play a major role promoting blast-induced ocular injuries, and that a single early
administration of anti-LPA antibodies provides significant protection.
AN - rayyan-553780130
AU - Arun, P.
AU - Rossetti, F.
AU - DeMar, J. C.
AU - Wang, Y.
AU - Batuure, A. B.
AU - Wilder, D. M.
AU - Gist, I. D.
AU - Morris, A. J.
AU - Sabbadini, R. A.
AU - Long, J. S. B.
DO - 10.3389/fneur.2020.611816
KW - Antibodies, Heterophile
PY - 2020
SN - 1664-2295
ST - Antibodies Against Lysophosphatidic Acid Protect Against Blast-Induced Ocular
Injuries
T2 - FRONTIERS IN NEUROLOGY
TI - Antibodies Against Lysophosphatidic Acid Protect Against Blast-Induced Ocular
Injuries
VL - 11
Y2 - 12 y3 - 15
ID - 8166
ER -
TY - JOUR
AB - Despite an increasing surge in application of nanoparticles in industries,
there is a serious lack of information concerning their impact on human health and
the environment. The present study investigated effects of molybdenum nanoparticles
(Mo NPs) injected intraperitoneally into Sprague-Dawley rats at different doses of
Mo NPs (5, 10, and 15 mg/kg BW per day) during a period of 28 days. Hematological
and biochemical parameters as well as sexual hormones and histopathological
examinations of the liver and testis were assessed and compared with control group.
The results showed that the serum levels of testosterone decreased significantly in
both groups of 10 and 15 mg (Mo NPs)/kg BW in comparison with the control group (p
< 0.05). However, there were insignificant differences observed in luteinizing
hormone (LH) levels and hematological parameters when compared with the control
group (p > 0.05). The results of liver enzymes showed that serum levels of
aspartate aminotransferase (AST) decreased significantly in both dosage groups of 5
and 10 mg/kg BW (Mo NPs) when compared with the control group (p < 0.05), and
significant decrease obtained in lactate dehydrogenase (LDH) levels at dose of 5
mg/kg BW in comparison with the control group (p < 0.05). The histopathological
examination of testis showed a decrease in number of Leydig cells. Also, the number
of chronic inflammatory cells increased in portal triad and parenchyma in liver
tissue of rats exposed to Mo NPs.
AN - rayyan-553780131
AU - Asadi, F.
AU - Mohseni, M.
AU - Noshahr, K. D.
AU - Soleymani, F. H.
AU - Jalilvand, A.
AU - Heidari, A.
DO - 10.1007/s12011-016-0765-5
IS - 1
KW - Rats
PY - 2017
SN - 0163-4984 1559-0720
SP - 50-56
ST - Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual
Hormones in Male Rats
TI - Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual
Hormones in Male Rats
VL - 175
Y2 - 1
ID - 8167
ER -
TY - JOUR
AB - Driven by the need to biosynthesized alternate biomedical agents to prevent
and treat infection, copper oxide nanoparticles (CuONPs) have surfaced as a
promising avenue. Cyanobacteria-derived synthesis of CuONPs is of substantive
interest as it offers an eco-friendly, cost-effective, and biocompatible route. In
the present study biosynthesized CuONPs were characterized and investigated
regarding their toxicity. Morphological analysis using TEM, SEM and AFM showed the
spherical particle size of 20.7 nm with 96% copper that confirmed the purity of
CuONPs. Biogenic CuONPs with IC50 value of 64.6 mu g ml(-1) showed 90% scavenging
of free radicals in superoxide radical scavenging assay. CuONPs showed enhanced
anti-inflammatory activity by 86% of protein denaturation with IC50 value of 89.9
mu g ml(-1). Biogenic CuONPs exhibited significant toxicity against bacterial
strains with lowest MIC value of 62.5 mu g ml(-1) for B. cereus and fungal strain
with a MIC value of 125 mu g ml(-1) for C. albicans. In addition CuONPs
demonstrated a high degree of synergistic interaction when combined with standard
drugs. CuONPs exhibited significant cytotoxicity against non-small cell lung cancer
with an IC50 value of 100.8 mu g ml(-1) for A549 and 88.3 mu g ml(-1) for the H1299
cell line with apoptotic activities. Furthermore, biogenic CuONPs was evaluated for
their photocatalytic degradation potential against methylene blue dye and were able
to removed 94% dye in 90 min. Free radical scavenging analysis suggested that
CuONPs assisted dye degradation was mainly induced by hydroxide radicals. Biogenic
CuONPs appears as an eco-friendly and cost effective photocatalyst for the
treatment of wastewater contaminated with synthetic dyes that poses threat to
aquatic biota and human health. The present study highlighted the blend of
biomedical and photocatalytic potential of Phormidium derived CuONPs as an
attractive approach for future applications in nanomedicine and bioremediation.
AN - rayyan-553780134
AU - Asif, N.
AU - Ahmad, R.
AU - Fatima, S.
AU - Shehzadi, S.
AU - Siddiqui, T.
AU - Zaki, A.
AU - Fatma, T.
DO - 10.1038/s41598-023-33360-3
IS - 1
KW - Copper
PY - 2023
SN - 2045-2322
ST - Toxicological assessment of Phormidium sp. derived copper oxide nanoparticles
for its biomedical and environmental applications
T2 - SCIENTIFIC REPORTS
TI - Toxicological assessment of Phormidium sp. derived copper oxide nanoparticles
for its biomedical and environmental applications
VL - 13
Y2 - 4 y3 - 17
ID - 8170
ER -
TY - JOUR
AB - Metal oxide nanoparticles are widely used in both consumer products and
medical applications, but the knowledge regarding exposure-related health effects
is limited. However, it is challenging to investigate nanoparticle interaction
processes with biological systems. The overall aim of this project was to improve
the possibility to predict exposure-related health effects of metal oxide
nanoparticles through interdisciplinary collaboration by combining workflows from
the pharmaceutical industry, nanomaterial sciences, and occupational medicine.
Specific aims were to investigate nanoparticle-protein interactions and possible
adverse immune reactions. Four different metal oxide nanoparticles; CeOx
nanocrystals with 5% or 14% Gd, Co3O4, and Fe2O3, were characterized by dynamic
light scattering and high-resolution transmission electron microscopy.
Nanoparticle-binding proteins were identified and screened for HLA-binding peptides
in silico. Monocyte interaction with nanoparticle-protein complexes was assessed in
vitro. Herein, for the first time, immunogenic properties of nanoparticle-binding
proteins have been characterized. The present study indicates that especially
Co3O4-protein complexes can induce both 'danger signals', verified by the
production of inflammatory cytokines and simultaneously bind autologous proteins,
which can be presented as immunogenic epitopes by MHC class II. The clinical
relevance of these findings should be further evaluated to investigate the role of
metal oxide nanoparticles in the development of autoimmune disease. The general
workflow identified experimental difficulties, such as nanoparticle aggregate
formation and a lack of protein-free buffers suitable for particle
characterization, protein analyses, as well as for cell studies. This confirms the
importance of future interdisciplinary collaborations.
AN - rayyan-553780137
AU - Assenhoj, M.
AU - Eriksson, P.
AU - Donnes, P.
AU - Ljunggren, S. A.
AU - Marcusson-Stahl, M.
AU - Du Rietz, A.
AU - Uvdal, K.
AU - Karlsson, H.
AU - Cederbrant, K.
DO - 10.1080/17435390.2021.1966115
IS - 8
KW - Monocytes
PY - 2021
SN - 1743-5390 1743-5404
SP - 1035-1058
ST - Protein interaction, monocyte toxicity and immunogenic properties of cerium
oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles
- an interdisciplinary approach
T2 - NANOTOXICOLOGY
TI - Protein interaction, monocyte toxicity and immunogenic properties of cerium
oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles
- an interdisciplinary approach
VL - 15
Y2 - 9 y3 - 14
ID - 8173
ER -
TY - JOUR
AB - Trichinellosis is a serious disease with no satisfactory treatment. We aimed
to assess the effect of myrrh (Commiphora molmol) and, for the first time, thyme
(Thymus vulgaris L.) against enteral and encysted (parenteral) phases of
Trichinella spiralis in mice compared with albendazole, and detect their effect on
inducible nitric oxide synthase (iNOS) expression. Oral administration of 500 mg/kg
of myrrh and thyme led to adult reduction (90.9%, 79.4%), while 1,000 mg/kg led to
larvae reduction (79.6%, 71.3%), respectively. Administration of 50 mg/kg of
albendazole resulted in adult and larvae reduction (94.2%, 90.9%). Positive
immunostaining of inflammatory cells infiltrating intestinal mucosa and submucosa
of all treated groups was detected. Myrrh-treated mice showed the highest iNOS
expression followed by albendazole, then thyme. On the other hand, both myrrh and
thyme-treated groups showed stronger iNOS expression of inflammatory cells
infiltrating and surrounding encapsulated T. spiralis larvae than albendazole
treated group. In conclusion, myrrh and thyme extracts are highly effective against
both phases of T. spiralis and showed strong iNOS expressions, especially myrrh
which could be a promising alternative drug. This experiment provides a basis for
further exploration of this plant by isolation and retesting the active principles
of both extracts against different stages of T. spiralis.
AN - rayyan-553780138
AU - Attia, Rasha A. H.
AU - Mahmoud, Abeer E.
AU - Farrag, Haiam Mohammed Mahmoud
AU - Makboul, Rania
AU - Mohamed, Mona Embarek
AU - Ibraheim, Zedan
DO - 10.1590/0074-02760150295
IS - 8
KW - Albendazole - thyme
Cytotoxicity tests
Enteral, parenteral phases
INOS
Limulus amoebocyte lysate assay
Myrrh
Trichinella spiralis
Mice
Nitric Oxide
LA - en
PY - 2015
SN - 0074-0276
SP - 1035-1041
ST - Effect of myrrh and thyme on Trichinella spiralisenteral and parenteral
phases with inducible nitric oxide expression in mice
T2 - Mem. Inst. Oswaldo Cruz
TI - Effect of myrrh and thyme on Trichinella spiralisenteral and parenteral
phases with inducible nitric oxide expression in mice
UR - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-
02762015000801035
VL - 110
Y2 - 12 y3 - 1
ID - 8174
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are of potential interest because of their
effective antibacterial and antiviral activities. Capping agents are used for
exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few
reports that have shown the usage of AgNPs for in-vivo antibacterial therapy.
Citrate-capped silver nanoparticles were synthesized chemically by citrate
reduction method; the size of Cit-AgNPs was determined by an atomic force
microscope (AFM) and was between 15 - 90 nm. Acinetobacter baumannii (A. baumannii)
isolates were the only sensitive species to Cit-AgNPs. MICs and MBC of Cit-AgNPs
were determined by using A. baumannii. The results showed an additive effect of
Cit-AgNPs. Four mice groups were infected with a sub-lethal dose of A. baumannii
intraperitoneally, IP. The single daily dose of Cit-AgNPs and imipenem plus Cit-
AgNPs combination were administered IP. Imipenem and phosphate buffer saline (PBS)
was used as positive control and negative control, respectively. Interestingly,
only the PBS-treated group showed growth of A. baumannii in the liver and spleen of
sacrificed mice. Histopathologically, Cit-AgNPs showed antibacterial activity and
had an additive effect when combined with imipenem in vivo and in vitro. Moreover,
the Cit-AgNPs showed dose-dependent activity and the organs differed in the
illumination of the toxicity effect of Cit-AgNPs even after high dose
administration. In conclusion, Cit-capped AgNPs had antibacterial activity against
multiple drug resistant (MDR) A. baumannii but not against K. pneumoniae and E.
coli. Cit-capped AgNPs increased the inhibition zone of imipenem in additive
effect; the minimum inhibitory concentration and the minimum bactericidal
concentration of Cit-capped AgNPs were relatively low. Cit-capped AgNPs eliminated
A. baumannii infection in vivo when it was given alone or in combination with
imipenem. The cytotoxicity of Cit-AgNPs was dose-dependent and the organs differed
in the illumination of the inflammatory effect of Cit-AgNPs after high dose
administration. It is not recommended to use Cit-capped AgNPs systemically despite
their valuable additive antibacterial effect especially with a high dose and the
combination with imipenem, Topical administration needs to be evaluated. Copyright
© Ibtesam Ghadban Auda, Istabreq Mohamed Ali Salman, Dalal Abed Al-Sattar, and
Jameelah Ghadban Oduha. This is an open-access article distributed under the terms
of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original author and
source are credited.
AN - rayyan-553780139
AU - Auda, I. G.
AU - Salman, I. M. A.
AU - Al-Sattar, D. A.
AU - Oduha, J. G.
DO - 10.5101/nbe.v13i3.p229-239
IS - 3
KW - Acinetobacter baumannii
Citrate capped silver nanoparticles
In vitro
In vivo
RecA gene
amoxicillin plus clavulanic acid
cefotaxime
ceftazidime
ciprofloxacin
citric acid
gentamicin
imipenem
phosphate buffered saline
piperacillin plus tazobactam
silver nanoparticle
Acinetobacter infection
animal experiment
animal model
animal tissue
antibiotic sensitivity
Article
atomic force microscopy
bacterial growth
bacterium isolate
combination drug therapy
controlled study
drug potentiation
drug synthesis
Escherichia coli
histopathology
illumination
in vitro study
in vivo study
Klebsiella pneumoniae
liver
minimum bactericidal concentration
mouse
multidrug resistant Acinetobacter baumannii
nonhuman
single drug dose
spleen
zone of inhibition
PY - 2021
SP - 229-239
ST - In-vivo and In-vitro anti-acinetobacter baumannii activity of citrate-capped
T2 - Nano Biomedicine and Engineering
TI - In-vivo and In-vitro anti-acinetobacter baumannii activity of citrate-capped
85115930017&doi=10.5101%2fnbe.v13i3.p229-
239&partnerID=40&md5=7b1e9146d8371b876fcca91d8a96f5ee
VL - 13
ID - 8175
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) are used in a variety of consumers' goods.
Their toxicological impact is currently intensely studied, mostly upon acute
exposure, but their intracellular dissolution and fate is rather poorly documented.
In this study, murine primary macrophages were exposed to a single high but non-
lethal dose of Ag-NPs or to repeated, low doses of Ag-NPs. Cells were either
collected immediately after acute exposure or after 72 h of recovery in the NP-free
exposure medium. Ag intracellular content and distribution were analyzed by
particle-induced X-ray emission, transmission electron microscopy coupled to
energy-dispersive spectroscopy analysis and inductively coupled plasma mass
spectrometry. In parallel, macrophage functionality as well as inflammatory and
thiol-responses were assessed after Ag-NP exposure. We show that Ag accumulation in
macrophages is similar upon acute and repeated exposure to Ag-NPs, and that Ag is
partly expelled from cells during the 72 h recovery stage. However, acute exposure
leads to a strong response of macrophages, characterized by reduced mitochondrial
membrane potential, phagocytic capacity and nitric oxide (NO) production upon
lipopolysaccharide (LPS) stimulation. Under this condition, we also show an
increased release of proinflammatory cytokines as well as a decreased release of
anti-inflammatory cytokines. This response is reversible since these biomarkers
reach their basal level after the recovery phase; and is much less intense in
repeatedly exposed cells. These results suggest that repeated exposure of
macrophages to Ag-NPs, which is a more realistic exposure scenario than acute
exposure, leads to significant Ag intracellular accumulation but a much less
intense toxicological response.
AN - rayyan-553780141
AU - Aude-Garcia, C.
AU - Villiers, F.
AU - Collin-Faure, V.
AU - Pernet-Gallay, K.
AU - Jouneau, P. H.
AU - Sorieul, S.
AU - Mure, G.
AU - Gerdil, A.
AU - Herlin-Boime, N.
AU - Carriere, M.
AU - Rabilloud, T.
DO - 10.3109/17435390.2015.1104738
IS - 5
KW - Macrophages
PY - 2016
SN - 1743-5390 1743-5404
SP - 586-596
ST - Different in vitro exposure regimens of murine primary macrophages to silver
nanoparticles induce different fates of nanoparticles and different toxicological
and functional consequences
T2 - NANOTOXICOLOGY
TI - Different in vitro exposure regimens of murine primary macrophages to silver
VL - 10
Y2 - 5 y3 - 27
ID - 8177
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) are used in a variety of consumers' goods.
Their toxicological impact is currently intensely studied, mostly upon acute
exposure, but their intracellular dissolution and fate is rather poorly documented.
In this study, murine primary macrophages were exposed to a single high but non-
lethal dose of Ag-NPs or to repeated, low doses of Ag-NPs. Cells were either
collected immediately after acute exposure or after 72 h of recovery in the NP-free
exposure medium. Ag intracellular content and distribution were analyzed by
particle-induced X-ray emission, transmission electron microscopy coupled to
energy-dispersive spectroscopy analysis and inductively coupled plasma mass
spectrometry. In parallel, macrophage functionality as well as inflammatory and
thiol-responses were assessed after Ag-NP exposure. We show that Ag accumulation in
macrophages is similar upon acute and repeated exposure to Ag-NPs, and that Ag is
partly expelled from cells during the 72 h recovery stage. However, acute exposure
leads to a strong response of macrophages, characterized by reduced mitochondrial
membrane potential, phagocytic capacity and nitric oxide (NO) production upon
lipopolysaccharide (LPS) stimulation. Under this condition, we also show an
increased release of proinflammatory cytokines as well as a decreased release of
anti-inflammatory cytokines. This response is reversible since these biomarkers
reach their basal level after the recovery phase; and is much less intense in
repeatedly exposed cells. These results suggest that repeated exposure of
macrophages to Ag-NPs, which is a more realistic exposure scenario than acute
exposure, leads to significant Ag intracellular accumulation but a much less
intense toxicological response.
AN - rayyan-553782063
AU - Villiers, F.
AU - Pernet-Gallay, K.
AU - Jouneau, P. H.
AU - Sorieul, S.
AU - Mure, G.
AU - Gerdil, A.
AU - Carrière, M.
AU - Rabilloud, T.
DO - 10.3109/17435390.2015.1104738
IS - 5
J2 - Nanotoxicology
KW - Animals
Cytokines/metabolism
Dose-Response Relationship, Drug
Lipopolysaccharides/pharmacology
Macrophages/*drug effects/immunology/ultrastructure
Membrane Potential, Mitochondrial/*drug effects
Metal Nanoparticles/chemistry/*toxicity
Mice
Phagocytosis/drug effects
Silver/chemistry/metabolism/*toxicity
Spectrometry, X-Ray Emission
Macrophages
LA - eng
N1 - a Laboratory of Chemistry and Biology of Metals , Université Grenoble Alpes ,
Grenoble , France .; b CEA Grenoble, iRTSV/CBM Laboratory of Chemistry and Biology
of Metals , Grenoble , France .; c CNRS UMR 5249, Laboratory of Chemistry and
Biology of Metals , Grenoble , France .; d CNRS, UMR5168; INRA, USC1359,
Laboratoire De Physiologie Cellulaire & Végétale, CEA, iRTSV, Université Grenoble
Alpes , Grenoble , France .; a Laboratory of Chemistry and Biology of Metals ,
Université Grenoble Alpes , Grenoble , France .; b CEA Grenoble, iRTSV/CBM
Laboratory of Chemistry and Biology of Metals , Grenoble , France .; c CNRS UMR
5249, Laboratory of Chemistry and Biology of Metals , Grenoble , France .; e
INSERM, U836 , Grenoble , France .; f Grenoble Institut Des Neurosciences,
Université Grenoble Alpes , Grenoble , France .; g INAC-SP2M, Université Grenoble
Alpes , Grenoble , France .; h CEA, INAC-SP2M , Grenoble , France .; i CNRS, IN2P3,
CENBG, UMR 5797 , Gradignan Cedex , France .; a Laboratory of Chemistry and Biology
of Metals , Université Grenoble Alpes , Grenoble , France .; b CEA Grenoble,
iRTSV/CBM Laboratory of Chemistry and Biology of Metals , Grenoble , France .; c
CNRS UMR 5249, Laboratory of Chemistry and Biology of Metals , Grenoble , France .;
j UMR 3685 IRAMIS NIMBE LEDNA, Bat 522, CEA Saclay , Cedex , France .; j UMR 3685
IRAMIS NIMBE LEDNA, Bat 522, CEA Saclay , Cedex , France .; k INAC-SCIB, Université
Grenoble Alpes , Grenoble , France , and.; l CEA, INAC-SCIB , Grenoble , France.; a
Laboratory of Chemistry and Biology of Metals , Université Grenoble Alpes ,
Grenoble , France .; b CEA Grenoble, iRTSV/CBM Laboratory of Chemistry and Biology
of Metals , Grenoble , France .; c CNRS UMR 5249, Laboratory of Chemistry and
Biology of Metals , Grenoble , France .
PY - 2016
SP - 586-96
ST - Different in vitro exposure regimens of murine primary macrophages to silver
T2 - Nanotoxicology
TI - Different in vitro exposure regimens of murine primary macrophages to silver
VL - 10
ID - 9977
ER -
TY - JOUR
AB - Nanoparticles (NPs), in particular noble metal nanoparticles, have been
incorporated into many therapeutic and biodiagnostic applications. While these
particles have many advantageous physical and optical properties, little is known
about their intrinsic intracellular effects in biological environments. Here, we
report the possible cell death mechanisms triggered in human oral squamous cell
carcinoma (HSC-3) cells after exposure to extracellular, cytoplasm, and nuclear
localized AuNPs and AgNPs. NP uptake and localization, cell viability, ATP levels,
modes of cell death, ROS generation, mitochondrial depolarization, and the levels
and/or translocation of caspase-dependent and caspase-independent proteins were
assessed under control and localized metal nanoparticle exposure. Exposure to AuNPs
resulted the adoption of a quiescent cellular state, as AuNPs caused a decrease in
intracellular ATP, but no change in viability or cell death populations. However,
AgNP exposure significantly reduced HSC-3 cell viability and increased apoptotic
populations, especially when localized at the cytoplasm and nucleus. Increased cell
death populations were linked to an increase in intracellular ROS generation.
Western blot analysis indicated cytoplasm localized AgNPs and nuclear localized
AgNPs utilized a caspase-independent apoptotic pathway that involved the nuclear
translocation of AIF and p38 MAPK proteins. These results demonstrate that the
degree of cytotoxicity increases as AgNPs move from extracellular localization to
nuclear localization, whereas changing AuNP localization does not trigger any
significant cytotoxicity. © 2014 Elsevier Ltd.
AN - rayyan-553780142
AU - Austin, L. A.
AU - Ahmad, S.
AU - Kang, B.
AU - Rommel, K. R.
AU - Mahmoud, M.
AU - Peek, M. E.
AU - El-Sayed, M. A.
DO - 10.1016/j.tiv.2014.11.003
IS - 4
KW - Apoptosis
Cytotoxicity
Gold nanoparticles
Organelle-targeted nanoparticles
Caspases
Cell Line, Tumor
Cell Nucleus
Cell Survival
Cytoplasm
Gold
Humans
Membrane Potential, Mitochondrial
Metal Nanoparticles
Necrosis
Particle Size
Peptides
Polyethylene Glycols
Reactive Oxygen Species
Silver
adenosine triphosphate
allograft inflammatory factor
caspase
cytokine
gold nanoparticle
mitogen activated protein kinase p38
silver nanoparticle
unclassified drug
gold
macrogol derivative
metal nanoparticle
peptide
silver
apoptosis
Article
cell death
cell nucleus
cell transport
cell viability
cellular distribution
controlled study
cytoplasm
cytotoxicity
depolarization
dielectric constant
energy yield
enzyme inhibition
exposure
extracellular space
HSC 3 cell line
human
human cell
hydrodynamics
mitochondrial membrane
mitochondrial membrane potential
mitochondrion
mouth squamous cell carcinoma
necrosis
nuclear localization signal
oxidative stress
protein function
protein transport
Western blotting
zeta potential
cell survival
chemistry
drug effects
metabolism
particle size
tumor cell line
PY - 2015
SP - 694-705
ST - Cytotoxic effects of cytoplasmic-targeted and nuclear-targeted gold and
silver nanoparticles in HSC-3 cells - A mechanistic study
T2 - Toxicology in Vitro
TI - Cytotoxic effects of cytoplasmic-targeted and nuclear-targeted gold and
silver nanoparticles in HSC-3 cells - A mechanistic study
84924308261&doi=10.1016%2fj.tiv.2014.11.003&partnerID=40&md5=e7b8047a82ffd2a9de5a9b
823b4e6d5a
VL - 29
ID - 8178
ER -
TY - JOUR
AB - Objective(s): Diabetes is related with the higher blood levels of liver
enzymes and inflammatory factors. Galega officinalis is used as a medicinal plant
for treatment of diabetes traditionally. In this work, silver nanoparticles (Ag-
NPs) were synthesized with green method using Galega officinalis extract. Materials
and Methods: 'I he synthesized green Ag-N Ps were characterized completely. Intact
or diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg green
synthesized Ag-NPs. Mean serum levels of glucose, hepatic enzymes and hematological
parameter were determined. Gene expression of tumor necrotic factor alpha (TNF-
alpha) was done by real-time PCR. Results: Synthesis of green synthesized Ag-NPs
was confirmed by FT-IR, XRD and UV-vis analyses. The FESEM and TEM images showed
spherical Ag-NPs with size of 25 nm. The hypoglycemic influence of Ag-NPs using
Galega officinalis extract is reported for the first time in this study. Blood
concentration of liver enzymes, urea, glucose, white blood cells count and TNF-
alpha mRNA levels in visceral adipose tissue significantly declined in diabetic
rats receiving Ag-NPs. Conclusion: The synthesized Ag-NPs using Galega officinalis
extract may improve complication of diabetes via preventing liver hepatocyte damage
and reducing inflammatory factors.
AN - rayyan-553780147
AU - Azimi, F.
AU - Mahmoudi, F.
AU - Amini, M. M.
DO - 10.22038/NMJ.2021.59391.1613
IS - 4
KW - Hypoglycemic Agents
Rats
PY - 2021
SN - 2322-3049 2322-5904
SP - 255-263
ST - Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic
effects in type 1 diabetic rats
T2 - NANOMEDICINE JOURNAL
TI - Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic
effects in type 1 diabetic rats
VL - 8
ID - 8183
ER -
TY - JOUR
AB - Azadirachta indica (Neem) is a large tree that is native to India and is
traditionally used due to its several properties, mainly to treat skin diseases, as
well as its "herbicidal" activity. Its bark, leaves, seeds, fruits and flowers are
widely used in medicinal treatment due to the presence of active secondary
metabolites with biological effects, mainly limonoids and tetranortriterpenoids,
such as azadirachtin. Thus, A. indica was studied in a variety of conditions, such
as anticancer, antiseptic, anti-inflammatory and chemopreventive agents, as well as
a biopesticide. Furthermore, differentiated cell tissue in A. indica cultivation
was reported to produce active metabolites for different purposes. However, only a
few studies have been developed regarding its potential use in cosmetics. For
instance, most studies explained the antimicrobial properties in health conditions,
such as acne, dandruff and personal health care. Here, we summarized not only the
most common cosmetic claims to treat acne but also mitigating other skin disorders
related to inflammatory and oxidant processes in recent in vivo studies and patents
to aid researchers and industrialists to select A. indica derivatives as novel
cosmetic ingredients.
AN - rayyan-553780148
AU - Baby, A. R.
AU - Freire, T. B.
AU - Marques, G. D.
AU - Rijo, P.
AU - Lima, F. V.
AU - de Carvalho, J. C. M.
AU - Rojas, J.
AU - Magalhaes, W. V.
AU - Velasco, M. V. R.
AU - Morocho-Jacome, A. L.
DO - 10.3390/cosmetics9030058
IS - 3
KW - Azadirachta
PY - 2022
SN - 2079-9284
ST - Azadirachta indica (Neem) as a Potential Natural Active for Dermocosmetic and
Topical Products: A Narrative Review
T2 - COSMETICS
TI - Azadirachta indica (Neem) as a Potential Natural Active for Dermocosmetic and
Topical Products: A Narrative Review
VL - 9
Y2 - 6
ID - 8184
ER -
TY - JOUR
AB - While inhalation represents one of the most likely routes of exposure, the
toxicity and response of nanoparticles at concentrations expected from such an
exposure are not well understood. Here we characterized the in vitro response of
human A549 adenocarcinomic alveolar epithelial cells following exposure to gold
(AuNP) and silver (AgNP) nanoparticles at levels approximating an occupational
exposure. Changes in neither oxidative stress nor cytotoxicity were significantly
affected by exposure to AgNPs and AuNPs, regardless of NP type (Ag vs. Au),
concentration, surface ligand (citrate or tannic acid), or size. An inflammatory
response was, however, observed in response to 20 nm AgNPs and 20 nm AuNPs, where
significant differences in the release of interleukin (IL)- 8 but not IL-6 were
observed. Additional data demonstrated that increased IL-8 secretion was strongly
dependent on both nanoparticle size and concentration. Overall these data suggest
that, while not acutely toxic, occupational exposure to AuNPs and AgNPs may trigger
a significant inflammatory response in alveolar epithelium. Moreover, the
differential responses in IL-8 and IL-6 secretion suggest that NPs may induce a
response pathway that is distinct from those commonly elicited by allergens and
pathogens. © Springer Science+Business Media Dordrecht 2012.
AN - rayyan-553780150
AU - Bachand, G. D.
AU - Allen, A.
AU - Bachand, M.
AU - Achyuthan, K. E.
AU - Seagrave, J. C.
AU - Brozik, S. M.
DO - 10.1007/s11051-012-1212-y
IS - 10
KW - Nanotoxicology
Occupational health
Particle toxicology
Toxicology
Cells
Industrial hygiene
Nanoparticles
citric acid
gold nanoparticle
interleukin 6
interleukin 8
silver nanoparticle
tannin
Alveolar epithelial cells
Differential response
Gold and silver nanoparticles
Inflammatory response
Occupational exposure
article
cancer cell culture
controlled study
cytokine production
cytokine release
cytotoxicity
human
human cell
in vitro study
inflammation
lung alveolus epithelium
occupational exposure
oxidative stress
particle size
priority journal
Humanities
Humanism
Humans
Occupational Exposure
Epithelial Cells
PY - 2012
ST - Cytotoxicity and inflammation in human alveolar epithelial cells following
exposure to occupational levels of gold and silver nanoparticles
T2 - Journal of Nanoparticle Research
TI - Cytotoxicity and inflammation in human alveolar epithelial cells following
exposure to occupational levels of gold and silver nanoparticles
84866479851&doi=10.1007%2fs11051-012-1212-
y&partnerID=40&md5=3ba3f5c6762e290bcdf99ccbd18affd1
VL - 14
ID - 8186
ER -
TY - JOUR
AB - Methemoglobin-forming drugs, such as sodium nitrite (NaNO2), may exacerbate
oxidative toxicity under certain chronic or acute hemolytic settings. In this
study, we evaluated markers of renal oxidative stress and injury in guinea pigs
exposed to extracellular hemoglobin (Hb) followed by NaNO2 at doses sufficient to
simulate clinically relevant acute methemoglobinemia. NaNO2 induced rapid and
extensive oxidation of plasma Hb in this model. This was accompanied by increased
renal expression of the oxidative response effectors nuclear factor erythroid 2-
derived-factor 2 (Nrf-2) and heme oxygenase-1 (HO-1), elevated non-heme iron
deposition, lipid peroxidation, interstitial inflammatory cell activation,
increased expression of tubular injury markers kidney injury-1 marker (KIM-1) and
liver-fatty acid binding protein (L-FABP), podocyte injury, and cell death.
Importantly, these indicators of renal oxidative stress and injury were minimal or
absent following infusion of Hb or NaNO2 alone. Together, these results suggest
that the exposure to NaNO2 in settings associated with increased extracellular Hb
may potentiate acute renal toxicity via processes that are independent of NaNO2
induced erythrocyte methemoglobinemia. Published by Elsevier Ireland Ltd.
AN - rayyan-553780153
AU - Baek, J. H.
AU - Zhang, X. Y.
AU - Williams, M. C.
AU - Hicks, W.
AU - Buehler, P. W.
AU - D'Agnillo, F.
DO - 10.1016/j.tox.2015.04.007
KW - Guinea Pigs
Oxidative Stress
Hemoglobin A, Glycosylated
Sodium Nitrite
PY - 2015
SN - 0300-483X
SP - 89-99
ST - Sodium nitrite potentiates renal oxidative stress and injury in hemoglobin
exposed guinea pigs
T2 - TOXICOLOGY
TI - Sodium nitrite potentiates renal oxidative stress and injury in hemoglobin
exposed guinea pigs
VL - 333
Y2 - 7 y3 - 3
ID - 8189
ER -
TY - CHAP
AB - Inorganic nanoparticles (NPs) either based on metal oxides (iron oxide,
cerium oxide, titanium dioxide, silicon dioxide, etc.) or metals (gold and silver)
have now wide applications. Consequently it increases the probability of unintended
exposure that could affect workers as well as the general population including
susceptible people. Inhalation, ingestion, and dermal contact are the main routes
of exposure. Before reaching the epithelial barrier lining the respiratory tract,
the digestive tract, or the skin, NPs get in contact with biological fluids and
become covered by molecules present in these fluids forming the so-called corona.
The fate and the effects of NPs may be different according to the corona
composition as the cell membrane does not interact directly with the NPs surface
but with the NP surrounded by its corona. Endocytosis has been shown to be an
important route of NPs uptake. However, the rate and mechanism of uptake seem to be
cell-type dependent, cell density-dependent and vary for NPs of different size,
charge, and other surface properties. Uptake is mostly an energy-dependent process,
dependent on NPs size, shape, and charge. There is also some evidence of NPs
exocytosis allowing NPs to cross epithelial barrier and enter systemic circulation.
Different in vitro models have been proposed showing potential of different NPs to
translocate. NPs biodistribution have been studied in different in vivo models
after intravenous injection, oral ingestion, intratracheal instillation, or
inhalation showing that smaller NPs can be better eliminated, but are also more
widespread in secondary organs. Inhalation studies underline that NPs mainly remain
at the site of exposure and only a low amount translocates. NPs health effects are
widely studied. Toxicological studies performed on animals by intratracheal
instillation have underlined that the most predominant effect of NPs is the
induction of lung inflammation characterized by the increase of immune cells,
frequently macrophages and neutrophils, in the bronchoalveolar lavage and the
increased release of pro-inflammatory mediators (cytokines and chemokines), and all
this effects are dependent on dose, size, surface reactivity, and NPs composition.
There is also evidence of some cardiovascular and neurologic effects of NPs. NPs
toxicity mainly results from their ability to induce an oxidative stress resulting
from the ability of NPs to directly or indirectly generate reactive oxygen species
(ROS). Some studies have shown the role of specific interactions between NPs and
proteins in cell activation or cell metabolism suggesting potential additional
pathways of toxicity independent of oxidative stress. A better knowledge about the
NPs properties involved in their toxicity is expected in order to propose NPs safe
by design. © 2013 Springer-Verlag London. All rights are reserved.
AN - rayyan-553780154
AU - Baeza-Squiban, A.
AU - Vranic, S.
AU - Boland, S.
DO - 10.1007/978-1-4471-4213-3_9
KW - Cells
Iron oxides
Metabolism
Metal nanoparticles
Molecular biology
Nanoparticles
Oxides
Silver
Titanium oxides
Toxicity
Energy-dependent process
Inflammatory mediators
Inorganic nanoparticle
Intratracheal instillation
Intravenous injections
Manufactured nanoparticles
Specific interaction
Cytology
PY - 2013
SP - 245-267
ST - Fate and health impact of inorganic manufactured nanoparticles
T2 - Nanomaterials: A Danger or a Promise? A Chemical and Biological Perspective
TI - Fate and health impact of inorganic manufactured nanoparticles
84928925806&doi=10.1007%2f978-1-4471-4213-
3_9&partnerID=40&md5=02708a6a4ac7a6bfd3371215d1c241e9
VL - 9781447142133
ID - 8190
ER -
TY - JOUR
AB - In addition to excellent biocompatibility and mechanical performance, the new
generation of bone and craniofacial implants are expected to proactively contribute
to the regeneration process and dynamically interact with the host tissue. To this
end, integration and sustained delivery of therapeutic agents has become a rapidly
expanding area. The incorporated active molecules can offer supplementary features
including promoting oteoconduction and angiogenesis, impeding bacterial infection
and modulating host body reaction. Major limitations of the current practices
consist of low drug stability overtime, poor control of release profile and
kinetics as well as complexity of finding clinically appropriate drug dosage. In
consideration of the multifaceted cascade of bone regeneration process, this
research is moving towards dual/multiple drug delivery, where precise control on
simultaneous or sequential delivery, considering the possible synergetic
interaction of the incorporated bioactive factors is of utmost importance. Herein,
recent advancements in fabrication of synthetic load bearing implants equipped with
various drug delivery systems are reviewed. Smart drug delivery solutions, newly
developed to provide higher tempo-spatial control on the delivery of the
pharmaceutical agents for targeted and stimuli responsive delivery are highlighted.
The future trend of implants with bone drug delivery mechanisms and the most common
challenges hindering commercialization and the bench to bedside progress of the
developed technologies are covered. " (C) 2016 Elsevier B.V. All rights reserved.
AN - rayyan-553780155
AU - Bagherifard, S.
DO - 10.1016/j.msec.2016.11.011
KW - Bone Regeneration
Regeneration
PY - 2017
SN - 0928-4931 1873-0191
SP - 1241-1252
ST - Mediating bone regeneration by means of drug eluting implants: From passive
to smart strategies
T2 - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
TI - Mediating bone regeneration by means of drug eluting implants: From passive
to smart strategies
VL - 71
Y2 - 2 y3 - 1
ID - 8191
ER -
TY - JOUR
AB - The present investigation aims to explore therapeutic potential of biogenic
synthesised silver nanoparticles (AgNPs) mediated using endophytic fungus extract
isolated from Tinospora cordifolia. The synthesised AgNPs characterised using
various physicochemical analyses. In FTIR, amide I and II bands of protein and
hydroxyl groups of phenolic hydroxyl confirming bioactive metabolites presence over
AgNPs surface. The mean size of AgNPs found 25–35 nm spherical in size. Further,
AgNPs demonstrated higher antibacterial, antioxidant, and anti-inflammatory
activity than endophyte fungal extract. Such high activity of AgNPs might be a
resultant of the propounding synergistic activity of silver nanoparticle and
endophyte bioactive metabolites over AgNPs surface that is confirmed by FTIR.
Moreover, antiproliferative activity in cervical and breast cancer cells was
demonstrated. Finally, AgNPs found cytocompatibility and hemocompatibility. Thus,
the results obtained provide apparent and multiple biological activities of
biogenic silver nanoparticles synthesised as possible multipurpose therapeutic
material with little systemic toxicity. © 2020 Informa UK Limited, trading as
Taylor & Francis Group.
AN - rayyan-553780156
AU - Bagur, H.
AU - Medidi, R. S.
AU - Somu, P.
AU - Choudhury, P. W. J.
AU - karua, C. S.
AU - Guttula, P. K.
AU - Melappa, G.
AU - Poojari, C. C.
DO - 10.1080/10667857.2020.1819089
IS - 3
KW - anti-inflammatory activity
Biogenic synthesis
multipurpose therapeutic agent
synergistic effect
Amides
Metabolites
Metal nanoparticles
Anti-inflammatory activity
Anti-proliferative activities
Physico-chemical analysis
Synergistic activity
Therapeutic materials
Therapeutic potentials
PY - 2022
SP - 167-178
ST - Endophyte fungal isolate mediated biogenic synthesis and evaluation of
biomedical applications of silver nanoparticles
T2 - Materials Technology
TI - Endophyte fungal isolate mediated biogenic synthesis and evaluation of
biomedical applications of silver nanoparticles
85091098023&doi=10.1080%2f10667857.2020.1819089&partnerID=40&md5=0c585f3a186c32918d
64757b1bbc59d5
VL - 37
ID - 8192
ER -
TY - JOUR
AB - Nanotechnology is a rapidly growing field having potential applications in
many areas. Nanoparticles (NPs) have been studied for cell toxicity,
immunotoxicity, and genotoxicity. Tetrazolium-based assays such as MTT, MTS, and
WST-1 are used to determine cell viability. Cell inflammatory response induced by
NPs is checked by measuring inflammatory biomarkers, such as IL-8, IL-6, and tumor
necrosis factor, using ELISA. Lactate dehydrogenase (LDH) assay is used for cell
membrane integrity. Different types of cell cultures, including cancer cell lines
have been employed as in vitro toxicity models. It has been generally agreed that
NPs interfere with either assay materials or with detection systems. So far,
toxicity data generated by employing such models are conflicting and inconsistent.
Therefore, on the basis of available experimental models, it may be difficult to
judge and list some of the more valuable NPs as more toxic to biological systems
and vice versa. Considering the potential applications of NPs in many fields and
the growing apprehensions of FDA about the toxic potential of nanoproducts, it is
the need of the hour to look for new internationally agreed free of bias
toxicological models by focusing more on in vivo studies. © 2016, Pasteur Institute
of Iran. All rights reserved.
AN - rayyan-553780159
AU - Bahadar, H.
AU - Maqbool, F.
AU - Niaz, K.
AU - Abdollahi, M.
IS - 1
KW - Cytotoxicity
In vitro
Metal nanoparticles
Review
Toxicology
Animals
Cell Line
Cell Survival
DNA Damage
Humans
Metal Nanoparticles
Nanostructures
Nanotechnology
aluminum oxide nanoparticle
carbon nanoparticle
gold nanoparticle
nanoparticle
silica nanoparticle
silver nanoparticle
titanium dioxide nanoparticle
ultrasmall superparamagnetic iron oxide
unclassified drug
metal nanoparticle
nanomaterial
apoptosis
blood brain barrier
cell structure
cell viability
chromosome damage
concentration (parameters)
cytotoxicity
disorders of mitochondrial functions
DNA adduct
DNA damage
DNA strand breakage
genotoxicity
human
lipid peroxidation
nanotechnology
nanotoxicology
nonhuman
oxidative stress
protein expression
animal
cell line
cell survival
drug effects
physiology
procedures
trends
PY - 2016
SP - 1-11
ST - Toxicity of nanoparticles and an overview of current experimental models
T2 - Iranian Biomedical Journal
TI - Toxicity of nanoparticles and an overview of current experimental models
84944755075&partnerID=40&md5=1c45a24b1c3af6c0ff01b0dcb9e7ed81
VL - 20
ID - 8195
ER -
TY - JOUR
AB - Background and Aims: Free-radical-mediated peroxidation of membrane lipids
and oxidative damage of DNA and proteins are believed to be associated with a
variety of chronic pathological complications such as cancer. The aim of this study
was to describe antioxidant and anti-inflammatory of silver nanoparticles (AgNPs)
synthesized using medicinal plant extract of Sage (Salvia officinalis). Materials
and Methods: AgNPs were synthesized using S. officinalis as reducer agents and
characterized using ultraviolet-visible, Fourier transform infrared spectroscopy,
particle seizer, and transmission electron microscopy. Toxicity of AgNPs on MCF-7
cells was investigated using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-
tetrazolium bromide (MTT) assay. Changes in expression of inflammation related
genes include cyclooxygenase-2, IL-8, and tumor necrosis factor-alpha (TNF-α) genes
were evaluated using semi-quantification reverse transcription-polymerase chain
reaction (RT-PCR). The antioxidant potential of capped AgNPs was assessed using 1,1
diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-
sulfonic acid (ABTS) radicals activity assay. Results: AgNPs successfully synthesis
with an average size of 16 nm and spherical. FTIR spectrum from plant extract and
AgNPs indicated the extract covered nanoparticles. AgNPs decreased cells viability
with inhibitory concentration (IC50) of 25 μg/ml and 20 μg/ml after 24 and 48 h,
respectively. To ascertain the anti-inflammatory genes expression, MCF-7 cells were
treated with 20 μg/ml AgNPs (concentration below of IC50 value according to MTT
assay). Semi-quantification RT-PCR results showed that AgNPs increased IL-8 and
TNF-α genes expression 28.76% and 42%, respectively, but suppressed cyclooxygenase-
2 gene expression with 20.5% comparing to control groups. Antioxidant assay of
green synthesized AgNPs coated by S. officinalis extract showed free radical
scavenging effect with IC50 of 830 and 800 μg/ml for DPPH and ABTS radicals,
respectively. Conclusion: The coated AgNPs with S. officinalis have promising
potential as a source for the development of chemotherapeutic agents in future. ©
2017 Annals of Tropical Medicine and Public Health | Published by Wolters Kluwer -
Medknow.
AN - rayyan-553780160
AU - Baharara, J.
AU - Ramezani, T.
AU - Mousavi, M.
AU - Asadi-Samani, M.
DO - 10.4103/ATMPH.ATMPH_174_17
IS - 5
KW - Anti-inflammatory
Antioxidant
Salvia officinalis
butylated hydroxyanisole
cyclooxygenase 2
glyceraldehyde 3 phosphate dehydrogenase
interleukin 8
Salvia officinalis extract
silver nanoparticle
ABTS radical scavenging assay
antioxidant assay
Article
cell survival
cell viability
comparative study
concentration response
controlled study
cytotoxicity
DPPH radical scavenging assay
drug synthesis
gene expression
green chemistry
human
human cell
IC50
inflammation
infrared spectroscopy
nanotoxicology
particle size
reverse transcription polymerase chain reaction
ultraviolet spectrophotometry
Antioxidants
PY - 2017
SP - 1265-1270
ST - Antioxidant and anti-inflammatory activity of green synthesized silver
nanoparticles using Salvia officinalis extract
T2 - Annals of Tropical Medicine and Public Health
TI - Antioxidant and anti-inflammatory activity of green synthesized silver
nanoparticles using Salvia officinalis extract
85033221553&doi=10.4103%2fATMPH.ATMPH_174_17&partnerID=40&md5=54a8d2a6b64838da16b8b
42533081c1a
VL - 10
ID - 8196
ER -
TY - JOUR
AB - There have been some successes in qualifying biomarkers and applying them to
drug development and clinical treatment of various diseases. A recent success is
illustrated by a collaborative effort among the US Food and Drug Administration,
the European Medicines Agency, and the pharmaceutical industry to provide a set of
seven preclinical kidney toxicity biomarkers for drug development. Other successes
include, but are not limited to, clinical biomarkers for cancer treatment and
clinical management of heart transplant patients. The value of fully qualified
surrogate endpoints in facilitating successful drug development is undisputed,
especially for diseases in which the traditional clinical outcome can only be
assessed in large, multi-year trials. Emerging biomarkers, including chemical
genomic or imaging biomarkers, and measurement of circulating tumor cells hold
great promise for early diagnosis of disease and as prognostic tests for managing
treatment of chronic diseases such as osteoarthritis, Alzheimer disease,
cardiovascular disease, and cancer. To advance the success of treating and managing
these diseases, efforts are needed to establish the temporal relationship between
changes in inflammatory or imaging biomarkers with the progression of the chronic
disease, and in the case of cancer, between the extent of circulating cancer cells
and tumor progression or remission.
AN - rayyan-553782415
AU - Bai, J. P.
AU - Bell, R.
AU - Buckman, S.
AU - Burckart, G. J.
AU - Eichler, H. G.
AU - Fang, K. C.
AU - Goodsaid, F. M.
AU - Jusko, W. J.
AU - Lesko, L. L.
AU - Meibohm, B.
AU - Patterson, S. D.
AU - Puig, O.
AU - Smerage, J. B.
AU - Snider, B. J.
AU - Wagner, J. A.
AU - Wang, J.
AU - Walton, M. K.
AU - Weiner, R.
DO - 10.1208/s12248-011-9265-x
IS - 2
J2 - AAPS J
KW - Animals
Biomarkers/*metabolism
Clinical Trials as Topic/methods
*Drug Design
Drug Evaluation, Preclinical/methods
Drug Industry/*methods
Humans
International Cooperation
Biological Markers
LA - eng
N1 - Office of Clinical Pharmacology, Center for Drug Evaluation and Research, US
Food and Drug Administration, Silver Spring, Maryland, USA. jbai.bai@fda.hhs.gov
PY - 2011
SP - 274-83
ST - Translational biomarkers: from preclinical to clinical a report of 2009
AAPS/ACCP Biomarker Workshop
T2 - The AAPS journal
TI - Translational biomarkers: from preclinical to clinical a report of 2009
AAPS/ACCP Biomarker Workshop
VL - 13
Y2 - 6
ID - 10322
ER -
TY - CONF
AB - The phytochemicals can act as reducing agents as well as nontoxic capping
materials of metal nanosilver. The objective of the present work was to fabricate
the synthesis of silver nanoparticle using aqueous bark extract of Saraca asoca as
reducing agent and testing the wound healing efficacy of the material by studying
the level of wound contraction and re-epithelialization and expression of
inflammatory factors like cytokines and different proinflammatory markers in
diabetic mice. The biocompatibility of the synthesized silver nanoparticle was
tested by cell cycle analysis, ROS generation, DAPI, comet assay. It was indicated
that the synthesized silver nanoparticles were nontoxic to normal mice in vivo.
Antibacterial properties of the silver formulation are comparable to different
antibiotics and more effective against Bacillus firmus and Staphylococcus
gallinarum of diabetic wound. SEM images and histopathological observations
revealed that the healing process was accelerated in the silver nanoparticle
treated group. The significant healing efficacy of phytofabricated nanoparticles
was evident by the observations of downregulation of proinflammatory cytokines such
as IL-1β, IL-6, and TNF-α, and upregulation of anti-inflammatory cytokine, IL-10. ©
2021, Springer Nature Singapore Pte Ltd.
AN - rayyan-553780161
AU - Bairagi, B.
AU - Nath, D.
DA - 2021
DO - 10.1007/978-981-15-7409-2_48
KW - Antibacterial efficacy
Diabetic wound
Saraca asoca
Wound healing
Wound Healing
Mice
SP - 475-484
ST - Wound Healing and Antimicrobial Property of Phytofabricated Silver
Nanoparticle by Saraca asoca Bark Extract on Diabetic Wound In Mice
T2 - Lecture Notes in Bioengineering
TI - Wound Healing and Antimicrobial Property of Phytofabricated Silver
Nanoparticle by Saraca asoca Bark Extract on Diabetic Wound In Mice
85092898345&doi=10.1007%2f978-981-15-7409-
2_48&partnerID=40&md5=7d3172e549fd90aa540e83100bada97f
ID - 8197
ER -
TY - JOUR
AB - Fungal metabolites, proteins, and enzymes have been rich sources of
therapeutics so far. Therefore, in this study, the hypha extract of a newly
identified noble fungus (Alternaria sp. with NCBI Accession number: MT982648) was
used to synthesize silver nanoparticles (F-AgNPs) to utilize against bacteria,
fungi, and lung cancer. F-AgNPs were characterized by using physical techniques,
including UV–visible spectroscopy, zeta potential, DLS, XRD, TEM, and HR-TEM. The
particles were found to be polydispersed and quasi-spherical in shape under TEM.
They had an average size of ~15 nm. The well dispersed particles were found to have
consistent crystallinity with cubic phase geometry under XRD and HR-TEM. The
presence of different functional groups on the surfaces of biosynthesized F-AgNPs
was confirmed by FTIR. The particle distribution index was found to be 0.447 with a
hydrodynamic diameter of ~47 d.nm, and the high value of zeta potential (−20.3 mV)
revealed the stability of the nanoemulsion. These particles were found to be active
against Staphylococcus aureus (multidrug resistance-MDR), Klebsiella pneumonia,
Salmonella abony, and Escherichia coli (MDR) with MIC50 10.3, 12.5, 22.69, and
16.25 µg/mL, respectively. Particles also showed inhibition against fungal strains,
including A. flavus, A. niger, T. viridens, and F. oxysporium. Their inhibition of
biofilm formation by the same panel of bacteria was also found to be very promising
and ranged from 16.66 to 64.81%. F-AgNPs also showed anticancer potential (IC50—
21.6 µg/mL) with respect to methotrexate (IC50—17.7 µg/mL) against lung cancer cell
line A549, and they did not result in any significant inhibition of the normal cell
line BEAS-2. The particles were found to alter the mitochondrial membrane
potential, thereby disturbing ATP synthesis and leading to high ROS formation,
which are responsible for cell membrane damage and release of LDH, intracellular
proteins, lipids, and DNA. A high level of ROS also elicits pro-inflammatory
signaling cascades that lead to programmed cell death by either apoptosis or
necrosis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780162
AU - Baker, A.
AU - Iram, S.
AU - Syed, A.
AU - Elgorban, A. M.
AU - Al-Falih, A. M.
AU - Bahkali, A. H.
AU - Khan, M. S.
AU - Kim, J.
DO - 10.3390/nano11123227
IS - 12
KW - AgNPS
Antibacterial
Antibiofilm
Anticancer
Fungus
Fungi
PY - 2021
ST - Potentially bioactive fungus mediated silver nanoparticles
T2 - Nanomaterials
TI - Potentially bioactive fungus mediated silver nanoparticles
85120002675&doi=10.3390%2fnano11123227&partnerID=40&md5=17ef61d357ad7a40a490118a470
f3d10
VL - 11
ID - 8198
ER -
TY - JOUR
AB - Biosynthesized low dimensional (<10 nm) nanoparticles are gaining great
attention in biomedical applications. Present research emphasizes a comparative
study of silver nanoparticles (Ag NPs) and Ag NPs decorated with reduced graphene
oxide nanocomposites (Ag NPs@rGO) synthesized from chemical and green methods, and
appraisal of their bioactivities and toxicity. We have used a unique sida acuta
leaf extract containing a multiplex combination of assorted bioactive compounds, as
a reducing agent of NPs formation from metal ions. We controlled the synthesis
conditions of molar ratio, pH, and reaction time to obtain performance enhanced Ag
NPs. Extremely-tiny spherical shaped Ag NPs (5 nm) with quite a delicate particle
dispersion over mono-layer graphene nanosheets was observed in green mediated Ag
NPs@rGO.The green synthesized Ag NPs@rGO outperformed to chemically prepared Ag NPs
and their composites for 500 μg/mL opposed to Methicillin-resistant Staphylococcus
aureus (MRSA) and Proteus mirabili bacteria. The G-Ag NPs and G-Ag NPs@rGO
demonstrated the highest cytotoxicity rate against MCF-7 cells at the half-maximal
inhibitory concentration (IC50) of 100 μg/mL and 108 μg/mL. Moreover, the green
mediated Ag NPs showed 99.18% anti-inflammatory, 96.09% anti-diabetic and 75.68%
antioxidant activities at 500 μg/mL according to their excellent temperature
stability. Ultimately, this study probes the novel approach of plant-mediated NPs
overcruel chemically incorporated NPs applied in pharmaceutical applications. ©
2023 Elsevier B.V.
AN - rayyan-553780165
AU - Balaji, V.
AU - Perumal, S.
AU - Palanisamy, S.
AU - Karuppaiah, M.
AU - Asaithambi, S.
AU - Velauthapillai, D.
AU - Kumar, P.
AU - Yuvakkumar, R.
AU - Ravi, G.
DO - 10.1016/j.jallcom.2023.171503
KW - MCF-7
MRSA
Reduced graphene oxide
Sidaacuta leaf
Tocopherols
PY - 2023
ST - Bio-inspired synthesis of silver nanoparticles and their nanocomposites for
antibacterial and anticancer activity: A comparative study
T2 - Journal of Alloys and Compounds
TI - Bio-inspired synthesis of silver nanoparticles and their nanocomposites for
antibacterial and anticancer activity: A comparative study
85166522611&doi=10.1016%2fj.jallcom.2023.171503&partnerID=40&md5=42300a4c3e59c63a4d
3eb6d39e20791d
VL - 966
ID - 8201
ER -
TY - JOUR
AB - The synthesis of metal nanoparticles by a green method is eco-friendly,
nontoxic and few benefits to the growing fields of research used inhibit bacteria
and cancer growth over the recent years. In this study, CuO NPs are synthesized
through Euphorbia hirta leaf extract. Formation of CuO NPs was confirmed by SPR
peak at UV spectrum. FTIR identified functional groups are responsible for
reduction and stabilization, GC-MS, phytochemicals present in the Euphorbia hirta
extract accountable for synthesizing CuO NPs. XRD confirmed crystalline nature, SEM
and TEM confirmed size and spherical morphology. Furthermore, CuO NPs effective
antibacterial activity to inhibit pathogens, strong biological activities regarding
antioxidant activity and anti-inflammatory activity and excellent anticancer
activity against the Ht-29 cell line. Also, good catalyst for the effective
degradation of methylene blue. The present study suggests that biosynthesized CuO
NPs through an extract from Euphorbia hirta may be used for therapeutic application
in future clinical studies.
AN - rayyan-553780166
AU - Balakrishnan, V.
AU - Thangaraj, K.
AU - Palani, M.
AU - Vaiyapuri, M.
DO - 10.1080/24701556.2021.1952260
IS - 6
KW - Copper
PY - 2022
SN - 2470-1556 2470-1564
SP - 809-818
ST - Green synthesis of copper oxide nanoparticles using Euphorbia hirta leaves
extract and its biological applications
T2 - INORGANIC AND NANO-METAL CHEMISTRY
TI - Green synthesis of copper oxide nanoparticles using Euphorbia hirta leaves
extract and its biological applications
VL - 52
Y2 - 6 y3 - 3
ID - 8202
ER -
TY - JOUR
AB - The aim of this study was to determine whether purinergic signaling is a
pathway associated with fumonisin B(1) (FB(1))-induced impairment of immune and
hemostatic responses. We also determined whether dietary supplementation with
diphenyl diselenide (Ph(2)Se(2)) prevents or reduces these effects. Splenic
nucleoside triphosphate diphosphohydrolase (NTPDase) activity for adenosine
triphosphate (ATP) and adenosine diphosphate (ADP) as substrates and total blood
thrombocytes counts were significant lower in silver catfish fed with FB1-
contaminated diets than in fish fed with a basal diet, while splenic adenosine
deaminase (ADA) activity and metabolites of nitric oxide (NOx) levels were
significant higher. Also, glutathione peroxidase (GPx) and superoxide dismutase
(SOD) activities were significant lower in silver catfish fed with FB(1)-
contaminated diets than in fish fed with a basal diet. Dietary supplementation with
3 mg Ph(2)Se(2)/kg of feed effectively modulated splenic NTPDase (ATP as
substrate), ADA, GPx and SOD activities, as well as NOx levels, and was partially
effective in the modulation of spleen NTPDase activity (ADP as substrate) and total
blood thrombocytes count. These data suggest that splenic purinergic signaling of
silver catfish fed with FB(1)-contaminated diets generates a pro-inflammatory
profile that contributes to impairment of immune and inflammatory responses, via
reduction of splenic ATP hydrolysis followed possible ATP accumulation in the
extracellular environment. Reduction of ADP hydrolysis associated with possible
accumulation in the extracellular environment can be a pathophysiological response
that restricts the hemorrhagic process elicited by FB(1) intoxication.
Supplementation with Ph(2)Se(2) effectively modulated splenic enzymes associated
with control of extracellular nucleotides (except ADP; that was partially
modulated) and nucleosides, thereby limiting inflammatory and hemorrhagic
processes.
AN - rayyan-553782198
AU - Baldissera, M. D.
AU - Souza, C. F.
AU - da Silva, H. N. P.
AU - Henn, A. S.
AU - Duarte, F. A.
AU - da Costa, S. T.
AU - Da Silva, A. S.
AU - Baldisserotto, B.
DO - 10.1016/j.cbpc.2019.108624
J2 - Comp Biochem Physiol C Toxicol Pharmacol
KW - Animal Feed/*analysis
Animals
Benzene Derivatives/*pharmacology
Blood Platelets
*Catfishes
Diet/veterinary
Fish Diseases/*chemically induced
Food Contamination
Fumonisins/*toxicity
Glutathione Peroxidase/metabolism
Nitrates/blood
Nitrites/blood
Organoselenium Compounds/*pharmacology
Signal Transduction
Spleen/*drug effects
Superoxide Dismutase/metabolism
LA - eng
N1 - Department of Physiology and Pharmacology, Universidade Federal de Santa
Maria, Santa Maria, RS, Brazil. Electronic address: mdbaldissera@mail.ufsm.br.;
Department of Physiology and Pharmacology, Universidade Federal de Santa Maria,
Santa Maria, RS, Brazil.; Department of Physiology and Pharmacology, Universidade
Federal de Santa Maria, Santa Maria, RS, Brazil.; Department of Chemistry,
Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.; Department of
Chemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.;
Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria,
RS, Brazil.; Department of Animal Science, Universidade do Estado de Santa
Catarina, Chapecó, RS, Brazil.; Department of Physiology and Pharmacology,
Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
PY - 2020
SN - 1532-0456 (Print)
SP - 108624
ST - Diphenyl diselenide modulates splenic purinergic signaling in silver catfish
fed diets contaminated with fumonisin B(1): An attempt to improve immune and
hemostatic responses
T2 - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
TI - Diphenyl diselenide modulates splenic purinergic signaling in silver catfish
fed diets contaminated with fumonisin B(1): An attempt to improve immune and
hemostatic responses
VL - 227
Y2 - 1
ID - 10108
ER -
TY - JOUR
AB - Thiamethoxam is a broad-spectrum pesticide widely used in agricultural
practice throughout the world. Worryingly, this pesticide is considered a potential
contaminant on the surface and underground water, being a significant risk to
aquatic ecosystems and humans. In this sense, we decided to evaluate the activity
of enzymes belonging to purinergic system, which is linked with regulation of
extracellular nucleotides and nucleosides, as adenosine triphosphate (ATP) and
adenosine (Ado) molecules involved in the regulation of immune and inflammatory
responses. Such as the neurotoxic effects of thiamethoxam remain poorly understood,
the aim of this study was to evaluate whether purinergic signaling may be
considered a potential target of thiamethoxam-induced neurotoxicity in silver
catfish (Rhamdia quelen). Brain ectonucleoside triphosphate diphosphohydrolase (ATP
as substrate) and 5′-nucleotidases activities were inhibited at 3.75 µg L−1 after
24 h of exposure and at 1.125 and 3.75 µg L−1 after 96 h of exposure compared with
the control group. On the other hand, brain adenosine deaminase activity was
stimulated at 3.75 µg L−1 after 24 h of exposure and at 1.125 and 3.75 µg L−1 after
96 h of exposure compared with the control group. Brain ATP levels increased at
3.75 µg L−1 after 24 h of exposure and at 1.125 and 3.75 µg L−1 after 96 h of
exposure compared with the control group, while the Ado levels decreased. The
enzymatic activity of the purinergic signaling did not return to control levels
after a 48-h recovery period, revealing the potential neurotoxic effects of
thiamethoxam. In summary, the brain purinergic signaling may be considered a
potential target for thiamethoxam-induced neurotoxicity in silver catfish. © 2018,
Springer Science+Business Media, LLC, part of Springer Nature.
AN - rayyan-553780169
AU - Souza, C. F.
AU - Golombieski, J. I.
AU - Seben, D.
AU - Sippert, L. R.
AU - Salbego, J.
AU - Marchesan, E.
AU - Zanella, R.
DO - 10.1007/s11010-018-3340-x
IS - 1
KW - Adenosine deaminase
Adenosine triphosphate
Inflammation
Pesticide
Adenosine
Adenosine Triphosphate
Animals
Brain
Catfishes
Fish Proteins
Neurotoxicity Syndromes
Signal Transduction
Thiamethoxam
5' nucleotidase
adenosine
adenosine deaminase
ectonucleoside triphosphate diphosphohydrolase
phosphatase
thiamethoxam
unclassified drug
fish protein
animal experiment
animal model
animal tissue
Article
catfish
controlled study
down regulation
enzyme activity
enzyme inhibition
female
hydrolysis
inflammation
male
neurotoxicity
nonhuman
Rhamdia quelen
signal transduction
animal
brain
disease model
drug effect
metabolism
pathology
toxicity and intoxication
PY - 2018
SP - 39-45
ST - Purinergic signaling as potential target of thiamethoxam-induced
neurotoxicity using silver catfish (Rhamdia quelen) as experimental model
T2 - Molecular and Cellular Biochemistry
TI - Purinergic signaling as potential target of thiamethoxam-induced
neurotoxicity using silver catfish (Rhamdia quelen) as experimental model
85042523204&doi=10.1007%2fs11010-018-3340-
x&partnerID=40&md5=839f9f9db943b3bcb160283eb2d4aa05
VL - 449
ID - 8205
ER -
TY - JOUR
AB - Evidence suggests the involvement of purinergic signaling, a mechanism
mediated by extracellular nucleotides and nucleosides, with the impairment of
immune and inflammatory responses in silver catfish (Rhamdia quelen) exposed to
trichlorfon. Plant-derived substances have been considered potent anti-inflammatory
agents due to effects on the purinergic system, such as the use of the flavonoid
rutin. The aim of this study was to determine whether a diet containing rutin is
able to prevent or reduce trichlorfon-induced impairment of immune responses
through alteration of the purinergic pathway. Spleen adenosine triphosphate (ATP)
levels were significantly higher in silver catfish exposed to 11 mg/L trichlorfon
for 48 h compared to the control group, while adenosine (Ado) levels were
significantly lower. Spleen ectonucleoside triphosphate diphosphohydrolase
(NTPDase) activity was significantly lower in silver catfish exposed to trichlorfon
compared to control group, while adenosine deaminase activity was significantly
higher. Spleen metabolites of nitric oxide, interleukin-1, and IL-6 were
significantly higher in silver catfish exposed to trichlorfon compared to control
group. Diet with 3 mg rutin/kg diet was able to prevent all the alterations
elicited by trichlorfon, except restoring spleen ATP levels. The purinergic
exposure signaling is involved in impairment of immune and inflammatory responses
in fish exposed to trichlorfon due to reduction in ATP hydrolyses and by an
increase in Ado deamination, leading to release of pro-inflammatory mediators. Use
of rutin-added diet exerted an essential role in protecting the silver catfish
spleen from trichlorfon-induced impairment on immune and inflammatory responses,
preventing all alterations on splenic purinergic signaling.
AN - rayyan-553782188
AU - Souza, C. F.
AU - Viana, A. R.
AU - da Silva, A. S.
DO - 10.1016/j.cbpc.2021.109006
KW - Adenosine Triphosphate/metabolism
Animals
Catfishes/*metabolism
Immunity/*drug effects
Insecticides/*toxicity
Rutin/*pharmacology
Spleen/drug effects
Trichlorfon/*toxicity
Trichlorfon
Pesticides
Dietary Supplements
Spleen
LA - eng
Maria, Santa Maria, RS, Brazil. Electronic address: matheus.baldissera@ufsm.br.;
Department of Physiology and Pharmacology, Universidade Federal de Santa Maria,
Santa Maria, RS, Brazil.; Department of Health Sciences, Franciscan University,
Santa Maria, RS, Brazil.; Department of Animal Science, Universidade do Estado de
Santa Catarina, Chapecó, SC, Brazil.; Department of Physiology and Pharmacology,
Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
PY - 2021
SN - 1532-0456 (Print)
SP - 109006
ST - Protective role of rutin dietary supplementation mediated by purinergic
signaling in spleen of silver catfish Rhamdia quelen exposed to organophosphate
pesticide trichlorfon
TI - Protective role of rutin dietary supplementation mediated by purinergic
signaling in spleen of silver catfish Rhamdia quelen exposed to organophosphate
pesticide trichlorfon
VL - 244
Y2 - 6
ID - 10098
ER -
TY - JOUR
AB - Suitable control of immune and inflammatory responses is a critical
determinant of fish health, and the cholinergic system, through the enzyme
acetylcholinesterase (AChE), displays an important role in these responses by
regulation on acetylcholine (ACh) levels. In fish, exposure to aflatoxin B-1
(AEB(1)) is associated with reduced immune responsiveness and immunosuppression,
but the pathways involved on the impairment of fish immune system during AFB(1)
exposure remains poorly understood. Thus, the aim of this study was to evaluate
whether AChE activity in splenic tissue and immune cells is able to modulate the
inflammatory and immune responses in silver catfish (Rhamdia quelen) fed with a
diet contaminated by AFB(1). AChE activity in spleen tissue, as well as in
peripheric and splenic lymphocytes were lower on days 14 and 21 post-feeding in
animals fed with AFB(1) contaminated feed compared to the control group. Based on
these evidences, the results indicate that AChE activity is capable to modulate
immune and inflammatory responses in tissue and immune cells of fish fed with a
diet containing AFB(1), leading to the development of an anti-inflammatory profile
in an attempt to improve immune response and reduce or prevent tissue damage due to
the inflammatory response.
AN - rayyan-553780170
AU - Souza, C. F.
AU - Zeppenfeld, C. C.
AU - Descovi, S. N.
AU - da Silva, A. S.
AU - Stefani, L. M.
DO - 10.1016/j.aquaculture.2018.12.023
PY - 2019
SN - 0044-8486 1873-5622
SP - 8-11
ST - Modulation of acetylcholinesterase activity exerts anti-inflammatory effect
in spleen and immune cells of fish fed with a diet contaminated by aflatoxin B-1
T2 - AQUACULTURE
TI - Modulation of acetylcholinesterase activity exerts anti-inflammatory effect
in spleen and immune cells of fish fed with a diet contaminated by aflatoxin B-1
VL - 502
Y2 - 3 y3 - 15
ID - 8206
ER -
TY - JOUR
AB - Alzheimer's disease is the most common cause of irreversible dementia,
affecting mostly the presenile and senile age, shaping a tragic profile in the
epilogue of the life of the suffering people. Due to the severity and the social
impact of the disease an ongoing research activity is in climax nowadays,
associated with many legal, social, ethical, humanitarian, philosophical and
economic considerations. From the neuropathological point of view the disease is
characterized by dendritic pathology, loss of synapses and dendritic spines,
affecting mostly selective neuronal networks of critical importance for memory and
cognition, such as the basal forebrain cholinergic system, the medial temporal
regions, the hippocampus and many neocortical association areas. Tau pathology
consisted of intracellular accumulation of neurofibrillary tangles of
hyperphosphorilated tau protein and accumulation of Aβ-peptide's deposits, defined
as neuritic plaques, are the principal neuropathological diagnostic criteria of the
disease. The neurotoxic properties of the oligomerics of the Aβ-peptide and tau
mediated neurodegeneration are among the main causative factors of impaired
synaptic plasticity, neuronal loss, dendritic alterations and tremendous synaptic
loss. The gradual degeneration of the organelles, particularly mitochondria, smooth
endoplasmic reticulum and Golgi apparatus, visualized clearly by electron
microscopy (EM), emphasize the importance of the oxidative stress and amyloid
toxicity in the pathogenetic cascade of the disease. The vascular factor may be an
important component of the whole spectrum of the pathogenesis of AD. It is of
substantial importance the concept that the structural alterations of the brain
capillaries, may contribute in the pathology of AD, given that the disruption of
the BBB may induce exacerbation of AD pathology, by promoting inflammation around
the blood capillaries and in the neuropile space diffusely. From the morphological
point of view, silver impregnation techniques revealed a marked tortuosity of the
capillaries in early cases of AD. In addition, the distance between two branch
points is longer in capillaries of AD brains, whereas the branch point density as
well as the ratio of the branch point density to astrocytic density is
substantially decreased in AD in comparison with age matched normal controls. EM
revealed, that the most frequent morphological alterations of the brain capillaries
in AD consist of thickness, splitting and duplication of the basement membrane,
reduction of the length of tight junctions, decrease of the number of tight
junctions per vessel length, associated as a rule, with morphological alterations
of the mitochondria of the endothelial cells, the pericytes and the perivascular
astrocytic processes. The number of the pinocytotic vesicles is substantially
increase in the endothelium of the brain capillaries in AD in comparison with age
matched normal controls. Endothelial cells play a very important role in the
transport systems in the brain. Subsequently, the dysfunction of the endothelial
cells and the disruption of the BBB may induce serious impairment in the transport
system. The dysfunction of the brain capillaries may result in releasing neurotoxic
factors, such as thrombin, pro-inflammatory cytokines, nitric oxide and leukocyte
adhesion molecules, and in abnormal regulation of Aβ-peptide homeostasis in the
brain. The impairment of the brain capillaries in structures of the brain, which
are crucial for the homeostatic equilibrium, such as the hypothalamic nuclei, may
induce autonomic dysfunction, which usually occur in the advanced stages of AD,
affecting dramatically the viability of the patients. Degeneration of the pericytes
is also observed emphasizing even more the importance of the vascular factor in AD.
Pericytes may serve as integrators, coordinators and effectors of blood-brain
barrier structure and maintenance, and play a key role in microvascular stability,
capillary density and angiogenesis. The correlation between AD pathology and
vascular patho ogy, at the level of brain capillaries and BBB, raises the rational
question, whether the efficient treatment of the vascular factor might be
beneficial for the patients who suffer from AD. It is reasonable that any
protection of the brain capillaries at the initial stages of the disease might
contribute in the abbreviation of the long chain of pathological alteration, which
occur following the disruption of the BBB, which serves as the essential interface
between the vascular system and the brain.
AN - rayyan-553782416
AU - Baloyannis, S. J.
J2 - Hell J Nucl Med
KW - Brain
Alzheimer Disease
LA - eng
N1 - Aristotelian University of Thessaloniki, Greece, Research Institute for
Alzheimer's disease, Aristotelian University, Greece. sibh844@otenet.gr.
PY - 2015
SN - 1790-5427 (Print)
SP - 152
ST - Brain capillaries in Alzheimer's disease
T2 - Hellenic journal of nuclear medicine
TI - Brain capillaries in Alzheimer's disease
VL - 18
Y2 - 9
ID - 10323
ER -
TY - JOUR
AB - Silver is proving to have a number of medicinal applications; as an
antiseptic, an antibacterial, and an anti-inflammatory, while any biological role
for it is currently unknown. Silver compounds and their therapeutic potentials are
under consideration from many research groups, while a number of early reviews
recording the advances of silver(i) chemistry are also available. However there is
no recent report on the screening for the antitumor potential of silver(i)
compounds. This review focuses upon results obtained on the anti-proliferative
activity of silver compounds in the past years. This survey shows that silver(i)
complexes containing various type of ligands such as carboxylic acids, amino acids,
nitrogen, phosphorus or sulfur donor ligands, exhibit selectivity against a variety
of cancer cells. The role of the coordination number, which is related to either
the stability or hydrophilicity-lipophilicity of a complex, is not clearly
elucidated within this review. © The Royal Society of Chemistry 2013.
AN - rayyan-553780173
AU - Banti, C. N.
AU - Hadjikakou, S. K.
DO - 10.1039/c3mt00046j
IS - 6
KW - Animals
Antineoplastic Agents
Cell Proliferation
Humans
Models, Biological
Silver Compounds
Amino acids
Diagnosis
Functional groups
Ligands
6 hydroxycoumarin 3 carboxylatosilver
acetic acid derivative
acetylcysteine derivative
acetylsalicylic acid derivative
adamantane derivative
alkane derivative
benzimidazole derivative
benzoic acid derivative
cisplatin
coumarin derivative
diamine derivative
dicarboxylic acid derivative
hydantoin derivative
hydrazide derivative
hydroxybenzoic acid derivative
malonic acid derivative
mesylic acid derivative
phenanthroline derivative
phosphine derivative
phosphonic acid derivative
pyrimidine derivative
silver derivative
sulfur derivative
thiophene derivative
thiosemicarbazone derivative
tryptophan derivative
unclassified drug
unindexed drug
Anti-inflammatories
Anti-proliferative
Anti-proliferative activities
Anti-tumor activities
Coordination number
Medicinal applications
Silver complexes
antiproliferative activity
binding affinity
cancer cell
carcinoma cell
cell viability
chronic myeloid leukemia
colon adenocarcinoma
cytostasis
DNA synthesis
drug cytotoxicity
drug screening
drug selectivity
drug stability
Ehrlich ascites tumor cell
fluorescence
human
hydrophilicity
IC 50
kidney carcinoma
lipophilicity
mastocytoma
melanoma
melanoma B16
neuroblastoma
nonhuman
nuclear magnetic resonance spectroscopy
ovary carcinoma
pancreas carcinoma
priority journal
R factor
review
squamous cell carcinoma
X ray crystallography
X ray diffraction
Silver compounds
PY - 2013
SP - 569-596
ST - Anti-proliferative and anti-tumor activity of silver(i) compounds
T2 - Metallomics
TI - Anti-proliferative and anti-tumor activity of silver(i) compounds
84878796988&doi=10.1039%2fc3mt00046j&partnerID=40&md5=aa56e4a2e3b0a68ef8b41026b2ded
fdc
VL - 5
ID - 8209
ER -
TY - JOUR
AB - The silver nanoparticles (AgNPs) prepared by chemical reduction with sodium
hypophosphite as a reducing agent and sodium hexametaphosphate as a stabilising
agent were highly cytotoxic against human cells (U-937 and HL-60). The aim of the
study was to determine the impact of selected antioxidants: ascorbic acid (AA),
gallic acid (GA), scavenger (trolox (TX)) and Ag + chelator (N-acetylcysteine, NAC)
on viability, modulation of inflammatory response and apoptosis index of cells
treated by AgNPs. Selected protectants added individually or together affects the
viability of cells treated by AgNPs (1 mg/L). The mixtures assuring the most
efficient defense against AgNPs were: AgNPs + TX + AA, AgNPs + GA + AA, AgNPs + TX
+ GA + AA and AgNPs + TX + GA + AA + NAC which synergistically interact in the
mixture. The greatest reduction in IL-6 and TNF-α levels was found for the mixture
containing AgNPs + TX + GA + AA. Mixture of this composition exhibited also the
strongest anti-apoptotic effect. Highly cytotoxic AgNPs may not damage human cells
if cytoprotectants are present. © 2019, © 2019 Informa UK Limited, trading as
AN - rayyan-553780179
AU - Barbasz, A.
AU - Czyżowska, A.
DO - 10.1080/08927022.2019.1566649
IS - 7
KW - antioxidants
human cells
Antioxidants
Cell death
Metal nanoparticles
Mixtures
Sodium compounds
Anti-apoptotic effects
Chemical reduction
Human cells
Sodium hexametaphosphate
Sodium hypophosphite
Stabilising agents
Ascorbic acid
Humanities
Humanism
Humans
PY - 2019
SP - 585-594
ST - Is there a way to protect human immune cells against nanocytotoxicity?
T2 - Molecular Simulation
TI - Is there a way to protect human immune cells against nanocytotoxicity?
85060184139&doi=10.1080%2f08927022.2019.1566649&partnerID=40&md5=b78b4c5dfa794e43f2
f05e25034fe488
VL - 45
ID - 8215
ER -
TY - JOUR
AB - The silver nanoparticles (AgNPs) prepared by chemical reduction with sodium
hypophosphite as a reducing agent and sodium hexametaphosphate as a stabilising
agent were highly cytotoxic against human cells (U-937 and HL-60). The aim of the
study was to determine the impact of selected antioxidants: ascorbic acid (AA),
gallic acid (GA), scavenger (trolox (TX)) and Ag+ chelator (N-acetylcysteine, NAC)
on viability, modulation of inflammatory response and apoptosis index of cells
treated by AgNPs. Selected protectants added individually or together affects the
viability of cells treated by AgNPs (1 mg/L). The mixtures assuring the most
efficient defense against AgNPs were: AgNPs + TX + AA, AgNPs + GA + AA, AgNPs + TX
+ GA + AA and AgNPs + TX + GA + AA + NAC which synergistically interact in the
mixture. The greatest reduction in IL-6 and TNF-alpha levels was found for the
mixture containing AgNPs + TX + GA + AA. Mixture of this composition exhibited also
the strongest anti-apoptotic effect. Highly cytotoxic AgNPs may not damage human
cells if cytoprotectants are present.
AN - rayyan-553780180
AU - Barbasz, A.
AU - Czyzowska, A.
DO - 10.1080/08927022.2019.1566649
IS - 7
KW - Humanities
Humanism
Humans
PY - 2019
SN - 0892-7022 1029-0435
SP - 585-594
ST - Is there a way to protect human immune cells against nanocytotoxicity?
T2 - MOLECULAR SIMULATION
TI - Is there a way to protect human immune cells against nanocytotoxicity?
VL - 45
Y2 - 5 y3 - 3
ID - 8216
ER -
TY - JOUR
AB - Gold nanoparticles (AuN) are one of the most investigated nanomaterials,
finding numerous applications from medicine to industry. AuN were obtained by
reduction of gold salts using tannic acid as a reducing agent. To detach the impact
of AuN onto cells of two lines human monocytic cells (U-937) and human
promyelocytic leukaemia cells (HL-60), the effects of postreaction residues (of
effluent from sol cleaning) and gold ions were also examined. It was demonstrated
that resistance of cells to the toxic action of AuN is dependent not only on
incubation time and dosage, but also on stages of cell differentiation. It was
found that after incubation of promonocytes U-937 with 25ppm AuN, the cell
viability decreased by 25% and of macrophages by 50%. Differentiated cells U-937
showed a significantly lower resistance than the not-differentiated cells. For HL-
60 cells, regardless of differentiation, cell viability decreased by approximately
20% after treatment with 25ppm AuN sol. It was noticed that U-937 exhibited higher
vulnerability to AuN than HL-60 cells. It was proved that AuN induced over a 15-
fold increase in nitric oxide and a decrease of intracellular glutathione levels
indicating the inflammatory response of cells. Even though gold ions did not show a
significant effect on cell viability, they caused fivefold increase of nitric oxide
level. The results show a higher cytotoxicity of AuN than gold ions. An overall
picture of the interaction of AuN with human cells of first defence line was
obtained. The results indicate that AuN may cause changes in the response of
phagocytes in inflammatory conditions.
AN - rayyan-553780181
AU - Barbasz, A.
AU - Ocwieja, M.
DO - 10.1080/17458080.2015.1096024
IS - 7
KW - Humanities
Humanism
Humans
Nanoparticles
PY - 2016
SN - 1745-8080 1745-8099
SP - 564-580
ST - Gold nanoparticles and ions - friends or foes? As they are seen by human
cells U-937 and HL-60
T2 - JOURNAL OF EXPERIMENTAL NANOSCIENCE
TI - Gold nanoparticles and ions - friends or foes? As they are seen by human
cells U-937 and HL-60
VL - 11
Y2 - 5 y3 - 2
ID - 8217
ER -
TY - JOUR
AB - The properties of silver nanoparticles (AgNPs) synthesized using compounds
exhibiting biological activity seem to constitute an interesting issue worthy of
examination. In these studies, two types of AgNPs were synthesized by a chemical
reduction method using well-known antioxidants: gallic acid (GA) and ascorbic acid
(AA). Transmission electron microscopy (TEM) and atomic force microscopy (AFM)
revealed that the AgNPs were spherical. The average size was equal to 26 ± 6 nm and
20 ± 7 nm in the case of ascorbic acid-silver nanoparticles (AAgNPs) and gallic
acid-silver nanoparticles (GAAgNPs), respectively. Surface-enhanced Raman
spectroscopy (SERS) confirmed that the AgNPs were not stabilized by pure forms of
applied antioxidants. Changes in mitochondrial activity and secretion of
inflammatory and apoptosis mediators after the exposure of human promyelocytic (HL-
60) and histiocytic lymphoma (U-937) cells to the AgNPs were studied to determine
the impact of stabilizing layers on nanoparticle toxicity. The GAAgNPs were found
to be more toxic for the cells than the AAgNPs. Their toxicity was manifested by a
strong reduction in mitochondrial activity and induction of the secretion of
interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and caspase-9. The addition
of pure antioxidants to the AgNP suspensions was found to influence their toxicity.
There was a significant positive effect in the case of the mixture of AA with
AAgNPs and GA with GAAgNPs. The results obtained suggest that the presence of
stabilizing agents adsorbed on the surface of AgNPs is the main factor in shaping
their toxicity. Nevertheless, the toxic effect can be also tuned by the
introduction of free antioxidant molecules to the AgNP suspensions. © 2021 John
Wiley & Sons, Ltd.
AN - rayyan-553780182
AU - Barbasz, A.
AU - Oćwieja, M.
AU - Piergies, N.
AU - Duraczyńska, D.
AU - Nowak, A.
DO - 10.1002/jat.4173
IS - 11
KW - antioxidants
ascorbic acid
cytotoxicity
gallic acid
HL-60
tumoral cells
U-937
Antioxidants
HL-60 Cells
Humans
Metal Nanoparticles
Microscopy, Atomic Force
Silver
Spectrum Analysis, Raman
U937 Cells
antioxidant
caspase 9
interleukin 6
nitric oxide
silver nanoparticle
stabilizing agent
metal nanoparticle
silver
adsorption
apoptosis
Article
biological activity
cell viability
controlled study
cytokine release
dispersity
drug cytotoxicity
drug structure
drug synthesis
electrophoretic mobility
histiocytic lymphoma
HL-60 cell line
human
human cell
inflammation
mitochondrion
MTT assay
particle size
pyroptosis
reduction (chemistry)
surface charge
surface enhanced Raman spectroscopy
surface property
suspension
U-937 cell line
voltammetry
X ray photoemission spectroscopy
chemistry
metabolism
Raman spectrometry
ultrastructure
PY - 2021
SP - 1863-1878
ST - Antioxidant-modulated cytotoxicity of silver nanoparticles
T2 - Journal of Applied Toxicology
TI - Antioxidant-modulated cytotoxicity of silver nanoparticles
85104670839&doi=10.1002%2fjat.4173&partnerID=40&md5=be7cdf006bdb4f11501bcebbdfa46a0
c
VL - 41
ID - 8218
ER -
TY - JOUR
AB - Copper(II) and silver(I) hyaluronane-based hydrogel complexes were
synthesised. The amount of metal ion uptaken by hydrogel was determined by atomic
absorption and the hydrogel-metal ion complexes were characterised by water-uptake
measurements, SEM-EDAX and FT-IR analysis. The coordination sites were identified
for both metal ions and the stability at various pH levels was determined. The
cytotoxicity of hydrogel metal ions was evaluated by using fibroblast (3T3) cells.
The copper(II) complex was "in vivo" tested and showed proangiogenic activity,
stimulating the growth of new vessels without inducing an inflammatory reaction.
The antibacterial activity of a silver complex was determined, showing that the
presence of silver ions drastically reduced the adhesion and proliferation of
Staphylococcus epidermidis.
AN - rayyan-553780184
AU - Barbucci, R.
AU - Leone, G.
AU - Magnani, A.
AU - Montanaro, L.
AU - Arciola, C. R.
AU - Peluso, G.
AU - Petillo, O.
DO - 10.1039/b205320a
IS - 10
KW - copper complex
hyaluronic acid
metal complex
metal ion
silver derivative
angiogenesis
animal cell
article
atomic absorption
complex formation
cytotoxicity
fibroblast
hydrogel
inflammation
mouse
nonhuman
pH
synthesis
X ray microanalysis
Hydrogel
Hydrogels
PY - 2002
SP - 3084-3092
ST - Cu2+- and Ag+-complexes with a hyaluronane-based hydrogel
T2 - Journal of Materials Chemistry
TI - Cu2+- and Ag+-complexes with a hyaluronane-based hydrogel
0036794482&doi=10.1039%2fb205320a&partnerID=40&md5=26c1218579fd16946180dc16128b0558
VL - 12
ID - 8219
ER -
TY - JOUR
AB - In recent decades, wound dressings have evolved from the simple gauze to
sophisticated, carefully designed functional materials which can enhance wound
healing and eliminate bacterial infections endangering the wounded area. With an
increasing number of multidrug-resistant strains, bacterial overgrowth of the
injured area poses a serious risk that can lead to severe conditions. Nanoparticles
can exhibit remarkable antibacterial properties thus incorporating them into
biocompatible matrices, effective antibacterial wound dressings can be fabricated.
Utilizing electrospinning, nanoparticles could be easily incorporated into fibrous
polymer meshes. Next to nanoparticles, electrospinning allows the simultaneous
encapsulation of small molecules as well, resulting in complex nanocomposite meshes
exhibiting antibacterial and anti-inflammatory and/or analgesic properties. In the
manuscript, we present a one-pot method for the synthesis of silver nanoparticles
(AgNP) in the presence of polysuccinimide (PSI) and a small molecule drug
(paracetamol), followed by the fabrication of an antibacterial wound dressing
system. Thorough characterization of both the AgNP-containing colloidal system and
the meshes were performed. Results reveal the stabilizing effect of PSI and
paracetamol enhancing the formation of a monodisperse colloidal system. Mechanical
studies confirm the reinforcing effect of silver-nanoparticles, and antibacterial
evaluation proves the applicability of the meshes. Drug-release measurement shows
prolonged three-step release kinetics. © 2020 The Authors
AN - rayyan-553780185
AU - Barczikai, D.
AU - Kacsari, V.
AU - Domokos, J.
AU - Szabó, D.
AU - Jedlovszky-Hajdu, A.
DO - 10.1016/j.molliq.2020.114575
KW - Antibacterial study
Drug release
Electrospinning
Nanocomposite
Nanoparticles
Paracetamol
Wound dressing
Aromatic compounds
Biocompatibility
Colloids
Controlled drug delivery
Drug products
Functional materials
Metal nanoparticles
Molecules
Targeted drug delivery
Tissue regeneration
Anti-inflammatories
Anti-inflammatory drugs
Antibacterial properties
Bacterial infections
Multidrug resistants
Reinforcing effects
Small-molecule drugs
Stabilizing effects
RNA, Messenger
Polyglactin 910
Poly(A)-Binding Protein II
Poly(ADP-ribose) Polymerases
Poly I-C
Poly(A)-Binding Proteins
Poly A-U
PY - 2021
ST - Interaction of silver nanoparticle and commonly used anti-inflammatory drug
within a poly(amino acid) derivative fibrous mesh
T2 - Journal of Molecular Liquids
TI - Interaction of silver nanoparticle and commonly used anti-inflammatory drug
within a poly(amino acid) derivative fibrous mesh
85094614203&doi=10.1016%2fj.molliq.2020.114575&partnerID=40&md5=313863a9485cd73e298
29c2aa1c40f5a
VL - 322
ID - 8220
ER -
TY - JOUR
AB - The presence of inflammation and demyelination in a central nervous system
(CNS) biopsy points towards a limited, yet heterogeneous group of pathologies, of
which multiple sclerosis (MS) represents one of the principal considerations.
Inflammatory demyelination has also been reported in patients with clinically
suspected primary central nervous system lymphoma (PCNSL), especially when steroids
had been administered prior to biopsy acquisition. The histopathological changes
induced by corticosteroid treatment can range from mild reduction to complete
disappearance of lymphoma cells. It has been proposed that in the absence of
neoplastic B cells, these biopsies are indistinguishable from MS, yet despite the
clinical relevance, no histological studies have specifically compared the two
entities. In this work, we analyzed CNS biopsies from eight patients with
inflammatory demyelination in whom PCNSL was later histologically confirmed, and
compared them with nine well defined early active multiple sclerosis lesions. In
the patients with steroid-treated PCNSL (ST-PCNSL) the interval between first and
second biopsy ranged from 3 to 32 weeks; all of the patients had received
corticosteroids before the first, but not the second biopsy. ST-PCNSL patients were
older than MS patients (mean age: ST-PCNSL: 62 ± 4 years, MS: 30 ± 2 years), and
histological analysis revealed numerous apoptoses, patchy and incomplete rather
than confluent and complete demyelination and a fuzzy lesion edge. The loss of
Luxol fast blue histochemistry was more profound than that of myelin proteins in
immunohistochemistry, and T cell infiltration in ST-PCNSL exceeded that in MS by
around fivefold (P = 0.005). Our data indicate that in the presence of extensive
inflammation and incomplete, inhomogeneous demyelination, the neuropathologist
should refrain from primarily considering autoimmune inflammatory demyelination
and, even in the absence of lymphoma cells, instigate close clinical follow-up of
the patient to detect recurrent lymphoma. © 2017 International Society of
Neuropathology
AN - rayyan-553780186
AU - Barrantes-Freer, A.
AU - Engel, A. S.
AU - Rodríguez-Villagra, O. A.
AU - Winkler, A.
AU - Bergmann, M.
AU - Mawrin, C.
AU - Kuempfel, T.
AU - Pellkofer, H.
AU - Metz, I.
AU - Bleckmann, A.
AU - Hernández-Durán, S.
AU - Schippling, S.
AU - Rushing, E. J.
AU - Frank, S.
AU - Glatzel, M.
AU - Matschke, J.
AU - Hartmann, C.
AU - Reifenberger, G.
AU - Müller, W.
AU - Schildhaus, H. U.
AU - Brück, W.
AU - Stadelmann, C.
DO - 10.1111/bpa.12496
IS - 2
KW - corticosteroids
demyelination
diffuse large B cell lymphoma
inflammation
multiple sclerosis
Adrenal Cortex Hormones
Aged
Apoptosis
Biopsy
Central Nervous System Neoplasms
Cohort Studies
Diagnosis, Differential
Female
Humans
Inflammation
Lymphoma
Male
Middle Aged
Multiple Sclerosis
Myelin Sheath
T-Lymphocytes
amyloid precursor protein
steroid
corticosteroid
adult
Article
autoimmune disease
autoimmune inflammatory demyelination
B lymphocyte
cancer patient
CD4 CD8 ratio
cell differentiation
cell infiltration
clinical article
corticosteroid therapy
differential diagnosis
disease course
female
follow up
human
human cell
human tissue
image analysis
immunohistochemistry
lymphoma cell
male
middle aged
primary central nervous system lymphoma
silver impregnation
tumor biopsy
tumor spheroid
aged
apoptosis
biopsy
central nervous system tumor
cohort analysis
lymphoma
myelin sheath
pathology
T lymphocyte
Demyelinating Diseases
PY - 2018
SP - 225-233
ST - Diagnostic red flags: steroid-treated malignant CNS lymphoma mimicking
T2 - Brain Pathology
TI - Diagnostic red flags: steroid-treated malignant CNS lymphoma mimicking
85017381807&doi=10.1111%2fbpa.12496&partnerID=40&md5=9a168cc0b5e29d3c455568f0602824
8d
VL - 28
ID - 8221
ER -
TY - JOUR
AB - Skin explants maintained in culture may represent a reliable model for in
vitro tests of the irritancy of chemicals. During the process of skin irritation
intracellular enzymes migrate into the culture medium. The amount of released
enzyme activity corresponds to the degree of skin damage. Skin of hairless mice
(hr/hr) has been found to be especially useful for this model. Histomorphology
demonstrated that the explants were almost identical to the in vivo situation. Skin
explants of hairless mice of 50 mm2 were used for the tests. The dermal side of the
skin is in contact with the medium whereas the substance is applied to the
epidermal side and incubated for 24 hr. As parameters for the membrane-damaging
effect, the enzymes lactate dehydrogenase and glutamic-oxaloacetate transaminase
were measured. The determination of the glucose utilization during the incubation
period gave additional information about the viability of the cultured skin.
Various chemicals were used. Histological examination complemented the biochemical
results and differentiated epidermal lesions, but was limited by the absence of
inflammatory reactions of the dermal part of the skin. Overall, in vitro skin
culture tests seem to be useful as screening tests prior to in vivo studies and for
the development of new formulations. © 1990.
AN - rayyan-553780189
AU - Bartnik, F. G.
AU - Pittermann, W. F.
AU - Mendorf, N.
AU - Tillman, U.
AU - Künstler, K.
DO - 10.1016/0887-2333(90)90067-4
IS - 4
KW - Animalia
alpha tocopherol
castor oil
dodecyl sulfate
phosphonic acid derivative
silver nitrate
sodium hydroxide
sulfuric acid
surfactant
animal cell
biological model
conference paper
female
histology
in vitro study
methodology
mouse
nonhuman
organ culture
priority journal
skin irritation
toxicity testing
Skin
PY - 1990
SP - 293-301
ST - Skin organ culture for the study of skin irritancy
TI - Skin organ culture for the study of skin irritancy
0024990485&doi=10.1016%2f0887-2333%2890%2990067-
4&partnerID=40&md5=68012ef87f408ee537b480eabc28b592
VL - 4
ID - 8224
ER -
TY - CONF
AB - Aloevera is one of the oldest medicinal plants and it possesses different
types of pharmacological properties including anti-microbial, anti-inflammatory,
anti-cancer and antioxidant activity. More over Aloevera contains 75 bioactive
compounds such as polyphenol, flavonoids, alkaloids, anthraquinone etc. Such
bioactive compounds can be used to reduce silver ions to produce silver
nanoparticles. In this study, we are trying to formulate bio-synthesised silver
nanoparticle (b-AgNps) by green chemistry approach. It is a very simple, efficient
and eco-friendly approach for silver nanoparticle synthesis that is formed by
reduction of silver nitrate (AgNO3) solution using Aloe vera leaf extract as a
reducing agent. Hydrothermal method was used to prepare b-AgNps using aloe vera
leaf extract as both reducing and stabilizing agent. Then b-AgNps were
characterized by FESEM, XRD, DLS and UV-VIS spectroscopy. Biological activity of b-
AgNps were evaluated by performing cytotoxic test (MTT assay) against breast cancer
cell lines and Fibroblast normal cell lines as well as screening antimicrobial
activity using agar well diffusion method. The results revealed that there was
significant amount of cell growth inhibition against breast cancer cell lines as
well as microbes compare to normal healthy fibroblast cell lines. © Published under
licence by IOP Publishing Ltd.
AN - rayyan-553780190
AU - Basak, P.
AU - Majumder, R.
AU - Jasu, A.
AU - Paul, S.
AU - Biswas, S.
DA - 2018
DO - 10.1088/1757-899X/410/1/012020
KW - Alovera
bio-synthesised silver nanoparticle (b-AgNps)
Breast cancer cell lines
Reducing agent
Antimicrobial agents
Bioactivity
Cell culture
Cytotoxicity
Diseases
Fibroblasts
Functional materials
Ketones
Metal ions
Metal nanoparticles
Microorganisms
Nitrogen compounds
Plants (botany)
Reducing agents
Ultraviolet visible spectroscopy
Anti-microbial activity
Anti-oxidant activities
Breast cancer cells
Green chemistry approaches
Pharmacological properties
Reduction of silver nitrates
Synthesised
Silver compounds
Anti-Infective Agents
Tocopherols
ST - Potential Therapeutic Activity of Bio-Synthesized Silver Nanoparticles as
Anticancer and Antimicrobial Agent
T2 - IOP Conference Series: Materials Science and Engineering
TI - Potential Therapeutic Activity of Bio-Synthesized Silver Nanoparticles as
Anticancer and Antimicrobial Agent
85054213504&doi=10.1088%2f1757-899X
%2f410%2f1%2f012020&partnerID=40&md5=3be0fa234f62b129da9e04445fc75fe2
VL - 410
ID - 8225
ER -
TY - JOUR
AB - Introduction: Periodontitis is a chronic inflammatory disease, resulting due
to host immune response against subgingival biofilm. Most conventional treatment
protocols aim to control the subgingival biofilm by mechanical means, such as
dental scaling and root planning, and frequently accompanied by antibacterial co-
adjuvant therapies, including antibiotics, antiseptics, or probiotics. Local drug
delivery facilitates administration of a lower dose of the drug to the target site,
but at higher concentration, thereby reducing systemic adverse effects and
toxicity. The present systematic review was conducted with the aim of identifying
and reporting nanoparticle based periodontal drug delivery systems, with a specific
focus on current trends and future perspectives in this field. Materials & methods:
Comprehensive literature search, restricted to published reports in English
language between January 2000 and February 2022, was done electronically and
manually. Search queries were addressed to the following electronic databases
including, PubMed (MEDLINE), Science Direct (Elsevier), Cochrane Library, Web of
Science (Clarivate Analytics) and Scholar (Google). Database search returned 780
results which were screened based on title, author names and publication dates, to
identify 13 studies fulfilling the review criteria. Results: Data from the 13
included studies were reviewed and tabulated, elaborating the type of nanoparticle
used, drug delivered and tissues/cells/subcellular components targeted by
periodontal drug delivery. While majority of the studies were conducted in vitro,
there were 3 in vivo studies and 3 clinical studies. Using nanotechnology for drug
delivery resulted in better inhibition of bacterial growth, inflammatory modulation
favoring resolution of periodontitis and capability for early tissue regeneration.
Conclusion: Recent developments in nanotechnology have enabled targeted local
delivery of drugs and anti-inflammatory biomolecules, in synergy with
nanoparticles, towards periodontal pathogens, inflammatory cells and periodontal
tissues. Further research evaluating clinical periodontal disease management
through nanoparticle based local drug delivery drugs is highly recommended. © 2022
The Authors
AN - rayyan-553780195
AU - Basudan, A. M.
DO - 10.1016/j.sdentj.2022.09.006
IS - 8
KW - Local drug delivery
Nanoparticles
Nanotechnology
Periodontal diseases
azithromycin
chitosan nanoparticle
clarithromycin
doxycycline
drug
gelatin
glutaraldehyde
gold nanoparticle
metronidazole
mevinolin
minocycline
nanocarrier
placebo
polyglactin
silicon dioxide
silver nanoparticle
Syzygium cumini extract
tetracycline
zinc
antibiotic therapy
bacterial growth
Cochrane Library
controlled study
flower
human
Medline
nanoencapsulation
nanomedicine
nonhuman
periodontal drug administration
periodontitis
plant seed
Review
ScienceDirect
search engine
systematic review
Syzygium cumini
tissue regeneration
Web of Science
Periodontics
PY - 2022
SP - 669-680
ST - Nanoparticle based periodontal drug delivery – A review on current trends and
future perspectives
T2 - Saudi Dental Journal
TI - Nanoparticle based periodontal drug delivery – A review on current trends and
future perspectives
85144245826&doi=10.1016%2fj.sdentj.2022.09.006&partnerID=40&md5=7658392a9d953fa45aa
cdea813052df9
VL - 34
ID - 8230
ER -
TY - JOUR
AB - Malva sylvestris is a plant commonly found in Europe, Asia, and Africa. The
leaves and flowers of this plant have been used for centuries in traditional
medicine to treat various ailments such as cough, cold, diarrhoea, and
constipation. Google Scholar, PubMed, Scopus, and Web of Science were used to
search for relevant material on the phytochemical profiling and pharmacologic
activities of Malva sylvestris. The techniques used in phytochemical profiling and
the pharmacologic activity of each compound were extracted from the included
studies, including in vitro, in vivo, and clinical studies. The phytochemical
analysis of Malva sylvestris revealed that the leaves and flowers are the most
commonly used parts of the plant and contain various bioactive compounds such as
flavonoids, mucilages, terpenoids, phenol derivatives, coumarins, sterols, tannins,
saponins, and alkaloids. These phytochemicals are responsible for the many
pharmacological activities of Malva sylvestris, such as anti-inflammatory,
antimicrobial, hepatoprotective, laxative, antiproliferative and antioxidant
properties. This review has presented an overview of the antinociceptive and anti-
inflammatory activities and the cytotoxic effects of Malva sylvestris on different
types of cancer cells. It has also summarised the work on developing copper oxide
nanoparticles using Malva sylvestris leaf extract and its potential use in food and
medicine. This review aims to highlight the traditional uses, phytochemistry,
pharmacological activities, and safety of Malva sylvestris. © 2022, The Author(s).
AN - rayyan-553780196
AU - Batiha, G. E. S.
AU - Tene, S. T.
AU - Teibo, J. O.
AU - Shaheen, H. M.
AU - Oluwatoba, O. S.
AU - Teibo, T. K. A.
AU - Al-kuraishy, H. M.
AU - Al-Garbee, A. I.
AU - Alexiou, A.
AU - Papadakis, M.
DO - 10.1007/s00210-022-02329-w
IS - 3
KW - Malva sylvestris
Pharmacological activities
Phytochemistry profiling
Safety
Traditional medicine
Malva
Phytochemicals
Phytotherapy
Plant Extracts
alkaloid
amino acid derivative
antiinfective agent
antinociceptive agent
antioxidant
coumarin derivative
cytotoxic agent
element
enzyme
fatty acid
flavonoid
laxative
liver protective agent
Malva sylvestris extract
metal nanoparticle
phenol derivative
phytosterol
pigment
plant extract
protein derivative
saponin
silver nanoparticle
tannin derivative
terpenoid
unclassified drug
vitamin
phytochemical
acute toxicity
agronomics
alternative medicine
antinociception
cancer cell
chemical analysis
chemical composition
chemical fingerprinting
crop
dietary fiber
drug activity
drug cytotoxicity
drug safety
ecology
economic aspect
flower
green chemistry
human
liver protection
medicinal plant
mucilage
nonhuman
phytochemistry
plant leaf
Review
synthesis
traditional medicine
veterinary medicine
chemistry
phytotherapy
PY - 2023
SP - 421-440
ST - The phytochemical profiling, pharmacological activities, and safety of malva
sylvestris: a review
T2 - Naunyn-Schmiedeberg's Archives of Pharmacology
TI - The phytochemical profiling, pharmacological activities, and safety of malva
sylvestris: a review
85142434570&doi=10.1007%2fs00210-022-02329-
w&partnerID=40&md5=a13f78eeb2764975922b68bf318ba127
VL - 396
ID - 8231
ER -
TY - JOUR
AB - Objective: To compare the rate of cell proliferation and expression of
antiapoptotic protein Bcl-2 between drug-induced gingival overgrowth (DIGO) and
clinical healthy gingiva (CHG) and to establish associations with histopathological
features. Material and Methods: Twenty specimens of DIGO and 20 CHG specimens were
submitted to morphological and immunohistochemical analysis by light microscopy.
Cell proliferation (Ki-67) and the expression of Bcl-2 were evaluated in epithelial
cells and spindle-shaped mononuclear cells of the connective tissue by establishing
the labeling index (LI). Results: In epithelial tissue, the mean LI for Ki-67 was
17.2% in DIGO and 21.71% in CHG (p = 0.137). The mean LIs for Bcl-2 in epithelial
tissue were 14.67% and 10.24% in DIGO and CHG, respectively (p = 0.026). In
connective tissue, DIGO and CHG specimens exhibited low LIs for Ki-67 and Bcl-2,
with mean values of less than 0.5% in both groups. No significant differences in
the LIs for Ki-67 or Bcl-2 in epithelial tissue were observed according to the
degree of collagenization, degree of vascularization and intensity of inflammatory
infiltration (p > 0.05). No significant correlations were observed between the LIs
for Ki-67 and Bcl-2 (p > 0.05). Conclusion: The present results suggest that the
pathogenesis of DIGO does not involve increased proliferation or decreased
apoptosis of fibroblasts. On the other hand, the morphological pattern of elongated
epithelial cristae observed in DIGO could mainly be due to the inhibition of
keratinocyte apoptosis and not to increased proliferation of these cells.
AN - rayyan-553780197
AU - Batista, Ana Luzia Araújo
AU - Mendonça, Angélica Kercya Pereira de
AU - Freitas, Roseana de Almeida
AU - Alves, Pollianna Muniz
AU - Godoy, Gustavo Pina
AU - Nonaka, Cassiano Francisco Weege
AU - Lins, Ruthinéia Diógenes Alves Uchôa
DO - 10.4034/PBOCI.2017.171.08
IS - 1
KW - Genes
Gingival Overgrowth
Ki-67 Antigen
bcl-2
LA - en
PY - 2017
SN - 1519-0501
SP - e3331-e3331
ST - Immunohistochemical Analysis of Cell Proliferation and Bcl-2 Expression in
Drug-Induced Gingival Overgrowth
T2 - Pesqui. bras. odontopediatria clín. integr
TI - Immunohistochemical Analysis of Cell Proliferation and Bcl-2 Expression in
Drug-Induced Gingival Overgrowth
UR - ["http://revista.uepb.edu.br/index.php/pboci/article/view/3331/pdf",
"http://fi-admin.bvsalud.org/document/view/zhyh8"]
VL - 17
Y2 - 1 y3 - 1
ID - 8232
ER -
TY - COMP
AB - Infection with Helicobacter pylori has been recognized as a cause of gastric
carcinoma. Although the neoplasia is always detected in adults, the infection
starts in childhood. It has been reported that early age at first infection is a
determinant of gastric cancer risk. In this study, we examined the histopathology
of the gastric mucosa in infected children from a population at high risk for
gastric cancer (Pasto, Colombia) and compared it with that of a lower-risk
population (New Orleans, LA). Gastric biopsies obtained from antrum and corpus were
stained with hematoxylin and eosin and Steiner's silver method. Immunohistochemical
stains were used to identify B lymphocytes (CD20), T lymphocytes (CD3 and CD8),
macrophages (CD68), and polymorphonuclear neutrophil myeloperoxidase. Morphometric
techniques were used to evaluate the immunohistochemical stains. In both
populations, the inflammatory lesions were seen predominantly in the antrum.
Compared with children from the lower-risk populations, children from the higher-
risk population exhibited more severe polymorphonuclear neutrophil infiltration,
stromal and intraepithelial lymphocyte infiltration, mucus depletion, and H. pylori
colonization density. Regenerative activity was significantly more marked in the
lower-risk population. Morphometric analysis of immunohistochemical stains showed
increased representation of T lymphocytes and macrophages in the higher-risk
population. Most T lymphocytes stained positive for CD8, a marker of
suppressor/cytotoxic cells. B lymphocytes were relatively more abundant in the
lower-risk population. The possibility that the aforementioned characteristics of
H. pylori infection in children are related to cancer risk in adults is discussed.
AN - rayyan-553782243
AU - Bedoya, A.
AU - Garay, J.
AU - Sanzón, F.
AU - Bravo, L. E.
AU - Bravo, J. C.
AU - Correa, H.
AU - Craver, R.
AU - Fontham, E.
AU - Du, J. X.
AU - Correa, P.
CY - United States
DO - 10.1053/hupa.2003.43
ET - 3
J2 - Hum Pathol
KW - B-Lymphocytes/pathology
Biopsy
CD3 Complex/analysis
CD8 Antigens/analysis
Child
Child, Preschool
Colombia/epidemiology
Epithelium/pathology
Female
Gastric Mucosa/pathology
Gastritis/*microbiology/*pathology
Helicobacter Infections/*pathology
*Helicobacter pylori
Humans
Infant
Louisiana
Macrophages/pathology
Male
Neutrophils/enzymology/pathology
Peroxidase/analysis
Risk Factors
Stomach Neoplasms/epidemiology/*microbiology
T-Lymphocytes/immunology/pathology
Stomach Neoplasms
Gastritis
Helicobacter pylori
LA - eng
N1 - Hospital Infantil, Pasto, Colombia.
PY - 2003
SN - 0046-8177 (Print)
SP - 206-13
ST - Histopathology of gastritis in Helicobacter pylori-infected children from
populations at high and low gastric cancer risk
T2 - Human pathology
TI - Histopathology of gastritis in Helicobacter pylori-infected children from
populations at high and low gastric cancer risk
VL - 34
Y2 - 3
ID - 10153
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have wide applications. Production of AgNPs can
be occurred through different method chemical, physical, and green methods. The
most popular methods are chemical approaches. Marine organisms exhibit a wide range
of bioactivity. The present study was designed to establish the biosynthesis of
silver nanoparticles from marine crustacean extract of the hard and soft parts of
male and female E. massavensis. The microstructure, morphology and optical
absorption properties of the nanoparticles were characterized by X-ray diffraction
(XRD), scanning electron microscopy (SEM) and UV-visible spectroscopy. The
formation of silver nanoparticles was confirmed by Uv-Vis absorption and the
spectra were observed plasmon bands between 441.79-462.74 nm. XRD results show that
the nanoparticles are crystalline in nature. SEM images detected the quasi-
spherical AgNPs morphological shape. Silver nanoparticles from marine crustacean
extract of the hard part of male E. massavensis (HM4) showed the best results in
morphology and particle size. Evaluation of the cytotoxicity of AgNPs (HM4) on
different cancer cell lines antiviral, anti-microbial, anti-diabetic, anti-
arthritic, anti-aging and anti-inflammatory properties were assessed. AgNPs
characterization may be introduced a promising applications in medical aspects. ©
2021 National Information and Documentation Center (NIDOC)
AN - rayyan-553780199
AU - Beltagy, D. M.
AU - Abdo, N. I.
AU - Samak, N. M.
AU - El-Khodary, G. M.
AU - Abdel-Aziz, K. K.
AU - Mona, M. H.
DO - 10.21608/ejchem.2021.70308.3549
IS - 8
KW - Bioapplications
Biosynthesis
Cytotoxicity
Marine crustacean
SEM
UV-Vis
XRD
Military Personnel
PY - 2021
SP - 4653-4662
ST - Beneficial bioapplications of silver nanoparticles synthesized by a marine
crustacean (Erugosquilla massavensis)
T2 - Egyptian Journal of Chemistry
TI - Beneficial bioapplications of silver nanoparticles synthesized by a marine
crustacean (Erugosquilla massavensis)
85112304638&doi=10.21608%2fejchem.2021.70308.3549&partnerID=40&md5=81e7942f785a5537
497283e0af09313d
VL - 64
ID - 8234
ER -
TY - JOUR
AB - Consumption of ethanol may have severe effects on human organs and tissues
and lead to acute and chronic inflammation of internal organs. The present study
aims at investigating the potential protective effects of three different extracts
prepared from the leaves, root, and stem of the sumac, Rhus tripartita, against
ethanol-induced toxicity and inflammation using intestinal cells as a cell culture
system, in vitro model of the intestinal mucosa. The results showed an induction of
cytotoxicity by ethanol, which was partially reversed by co-administration of the
plant extracts. As part of investigating the cellular response and the mechanism of
toxicity, the role of reduced thiols and glutathione-S-transferases were assessed.
In addition, intestinal cells were artificially imposed to an inflammation state
and the anti-inflammatory effect of the extracts was estimated by determination of
interleukin-8. Finally, a detailed characterization of the contents of the three
plant extracts by high resolution Nuclear Magnetic Resonance (NMR) spectroscopy and
mass spectrometry revealed significant differences in their chemical compositions.
(C) 2017 Elsevier B.V. All rights reserved.
AN - rayyan-553780200
AU - Ben Barka, Z.
AU - Grintzalis, K.
AU - Polet, M.
AU - Heude, C.
AU - Sommer, U.
AU - Ben Miled, H.
AU - Ben Rhouma, K.
AU - Mohsen, S.
AU - Tebourbi, O.
AU - Schneider, Y. J.
DO - 10.1016/j.jpba.2017.12.032
PY - 2018
SN - 0731-7085 1873-264X
SP - 347-354
ST - A combination of NMR and liquid chromatography to characterize the protective
effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on
intestinal cells
T2 - JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
TI - A combination of NMR and liquid chromatography to characterize the protective
effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on
intestinal cells
VL - 150
Y2 - 2 y3 - 20
ID - 8235
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been reported as stressors for the
bivalves' immune system at different regulatory levels, impacting the detection
step and receptors, and other mediators, as well as effector molecules. However,
studies on how AgNPs impact the transmission of signals from receptors and whether
they have an effect on mediators and transcription factors are still scarce. This
study aims to investigate the effect of 12 hours of in vivo exposure to 100 mu g/L
of AgNPs on the gene expression of the cytosolic adaptor Myeloid, the
differentiation protein 88 (MgMyD88-b), and the interferon regulatory factor (Me4-
IRF) in the gills and digestive gland of Mytilus galloprovincialis, before and
after blocking two major uptake pathways of nanoparticles (clathrin- and caveolae-
mediated endocytosis). The results illustrate a tissue-specific gene expression of
the MgMyD88-b and the Me4-IRF in the gills and digestive gland of M.
galloprovincialis. In the gills, AgNPs did not significantly impact the expression
of the two genes. However, blocking the caveolae-mediated endocytosis decreased the
expression of Me4-IRF. However, inhibition of clathrin-mediated endocytosis in the
digestive gland recorded a significant decrease in the expression of MgMyD88-b.
Overall, the inhibition of the AgNPs' uptake routes have highlighted their
potential interference with the immune response through the studied mediators'
genes, which need to be studied further in future investigations.
AN - rayyan-553780201
AU - Ben Younes, R.
AU - Bouallegui, Y.
AU - Fezai, O.
AU - Mezni, A.
AU - Touaylia, S.
AU - Oueslati, R.
DO - 10.1080/01480545.2021.1945128
IS - 5
KW - Gene Expression
Cytosol
PY - 2022
SN - 0148-0545 1525-6014
SP - 2371-2378
ST - Silver nanoparticles' impact on the gene expression of the cytosolic adaptor
MyD-88 and the interferon regulatory factor IRF in the gills and digestive gland of
mytilus galloprovincialis
T2 - DRUG AND CHEMICAL TOXICOLOGY
TI - Silver nanoparticles' impact on the gene expression of the cytosolic adaptor
MyD-88 and the interferon regulatory factor IRF in the gills and digestive gland of
mytilus galloprovincialis
VL - 45
Y2 - 9 y3 - 3
ID - 8236
ER -
TY - JOUR
AB - Background: Diesel exhaust is carcinogenic and exposure to diesel particles
cause health effects. We investigated the toxicity of diesel exhaust particles
designed to have varying physicochemical properties in order to attribute health
effects to specific particle characteristics. Particles from three fuel types were
compared at 13% engine intake O2 concentration: MK1 ultra low sulfur diesel (DEP13)
and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed
methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur
diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O2 concentration. We
evaluated physicochemical properties and histopathological, inflammatory and
genotoxic responses on day 1, 28, and 90 after single intratracheal instillation in
mice compared to reference diesel particles and carbon black. Results: Moderate
variations were seen in physical properties for the five particles: primary
particle diameter: 15-22 nm, specific surface area: 152-222 m2/g, and count median
mobility diameter: 55-103 nm. Larger differences were found in chemical
composition: organic carbon/total carbon ratio (0.12-0.60), polycyclic aromatic
hydrocarbon content (1-27 μg/mg) and acid-extractable metal content (0.9-16 μg/mg).
Intratracheal exposure to all five particles induced similar toxicological
responses, with different potency. Lung particle retention was observed in DEP13
and HVO13 exposed mice on day 28 post-exposure, with less retention for the other
fuel types. RME exposure induced limited response whereas the remaining particles
induced dose-dependent inflammation and acute phase response on day 1. DEP13
induced acute phase response on day 28 and inflammation on day 90. DNA strand break
levels were not increased as compared to vehicle, but were increased in lung and
liver compared to blank filter extraction control. Neutrophil influx on day 1
correlated best with estimated deposited surface area, but also with elemental
carbon, organic carbon and PAHs. DNA strand break levels in lung on day 28 and in
liver on day 90 correlated with acellular particle-induced ROS. Conclusions: We
studied diesel exhaust particles designed to differ in physicochemical properties.
Our study highlights specific surface area, elemental carbon content, PAHs and ROS-
generating potential as physicochemical predictors of diesel particle toxicity. ©
2020 The Author(s).
AN - rayyan-553780202
AU - Bendtsen, K. M.
AU - Gren, L.
AU - Malmborg, V. B.
AU - Shukla, P. C.
AU - Tunér, M.
AU - Essig, Y. J.
AU - Krais, A. M.
AU - Clausen, P. A.
AU - Berthing, T.
AU - Loeschner, K.
AU - Jacobsen, N. R.
AU - Wolff, H.
AU - Pagels, J.
AU - Vogel, U. B.
DO - 10.1186/s12989-020-00369-9
IS - 1
KW - Diesel exhaust particles - ultrafine particles
Exhaust gas recirculation
Renewable diesel fuels
Toxicity
Animals
Carbon
Carcinogens
DNA Damage
Gasoline
Lung
Mice
Mice, Inbred C57BL
Particulate Matter
Polycyclic Aromatic Hydrocarbons
Vehicle Emissions
arsenic
black carbon
cadmium
carbon monoxide
cesium
chromium
cobalt
copper
diesel fuel
hydrotreated vegetable oil
indium
iron
manganese
nickel
nitrogen oxide
organic carbon
polycyclic aromatic hydrocarbon
rapeseed methyl ester
rapeseed oil
renewable fuel
rubidium
selenium
silver
strontium
sulfur
unclassified drug
vanadium
vegetable oil
volatile organic compound
carbon
carcinogen
gasoline
acute phase response
animal cell
animal experiment
animal tissue
Article
C57BL 6 mouse
concentration (parameter)
controlled study
count median mobility diameter
diesel engine
diesel particulate matter
DNA strand breakage
extraction
female
genotoxicity
histopathology
inflammation
inhalation
liver tissue
lung parenchyma
lung toxicity
mouse
nonhuman
particle size
particulate matter exposure
physical chemistry
physical parameters
priority journal
surface area
toxicity testing
trachea
animal
C57BL mouse
DNA damage
exhaust gas
lung
particulate matter
toxicity
PY - 2020
ST - Particle characterization and toxicity in C57BL/6 mice following instillation
of five different diesel exhaust particles designed to differ in physicochemical
properties
TI - Particle characterization and toxicity in C57BL/6 mice following instillation
of five different diesel exhaust particles designed to differ in physicochemical
properties
85089261623&doi=10.1186%2fs12989-020-00369-
9&partnerID=40&md5=f65dfd261ca16e0db9d207afca3e15ab
VL - 17
ID - 8237
ER -
TY - JOUR
AB - Purpose: The leaves and flowering stem of Origanum vulgare contain essential
oils, flavonoids, phenolic acids and anthocyanins. We propose a new, simple, one-
pot, O. vulgare extract (OVE) mediated green synthesis method of biocompatible gold
nanoparticles (AuNPs) possessing improved antioxidant, antimicrobial and plasmonic
properties. Materials and methods: Different concentrations of OVEs were used to
reduce gold ions and to synthetize biocompatible spherical AuNPs. Their morphology
and physical properties have been investigated by means of transmission electron
microscopy, ultraviolet-visible absorption spectroscopy, photon correlation
spectroscopy and Fourier transform infrared spectroscopy, whereas their plasmonic
properties have been tested using surface-enhanced Raman spectroscopy (SERS). The
antioxidant properties of nanoparticles (NPs) have been evaluated by 2,2-diphenyl-
1-picrylhydrazyl radical scavenging assay, and the antimicrobial tests were
performed using the disk diffusion assay. Their cytotoxicity has been assessed by
means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: The experimental results confirmed the successful synthesis of
biocompatible, spherical, plasmonic NPs having a mean diameter of similar to 40 nm
and an outstanding aqueous stability. This new class of NPs exhibits a very good
antioxidant activity and presents interesting inhibitory effects against
Staphylococcus aureus and Candida albicans. Due to their plasmonic properties,
AuNPs are used as SERS substrates for the detection of a test molecule (methylene
blue) up to a concentration of 10(-7) M and a pharmaceutical compound (propranolol)
in solution. Cytotoxicity assays revealed that AuNPs are better tolerated by normal
human dermal fibroblast cells, while the melanoma cancer cells are more sensitive.
Conclusion: The biocompatible AuNPs synthetized using OVEs showed significant
bactericidal and antimycotic activities, the most sensitive microorganisms being S.
aureus and C. albicans, both commonly involved in various dermatological
infections. Moreover, the significant antioxidant effect might recommend their use
for protective and/or preventive effect in various skin inflammatory conditions,
including the reduction in side effects in dermatological infections. Meanwhile,
the as-synthesized biocompatible AuNPs can be successfully used as SERS substrates
for the detection of pharmaceutical compounds in aqueous solutions.
AN - rayyan-553780203
AU - Benedec, D.
AU - Oniga, I.
AU - Cuibus, F.
AU - Sevastre, B.
AU - Stiufiuc, G.
AU - Duma, M.
AU - Hanganu, D.
AU - Iacovita, C.
AU - Stiufiuc, R.
AU - Lucaciu, C. M.
DO - 10.2147/IJN.S149819
KW - Antioxidants
Origanum
PY - 2018
SN - 1178-2013
SP - 1041-1058
ST - Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles
simultaneously possessing plasmonic, antioxidant and antimicrobial properties
TI - Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles
simultaneously possessing plasmonic, antioxidant and antimicrobial properties
VL - 13
ID - 8238
ER -
TY - JOUR
AB - The pathological processes that lead to long-term consequences of multiple
concussions are unclear. Primary mechanical damage to axons during concussion is
likely to contribute to dysfunction. Secondary damage has been hypothesized to be
induced or exacerbated by inflammation. The main inflammatory cells in the brain
are microglia, a type of macrophage. This research sought to determine the
contribution of microglia to axon degeneration after repetitive closed-skull
traumatic brain injury (rcTBI) using CD11b-TK (thymidine kinase) mice, a
valganciclovir-inducible model of macrophage depletion. Low-dose (1 mg/mL)
valganciclovir was found to reduce the microglial population in the corpus callosum
and external capsule by 35% after rcTBI in CD11b-TK mice. At both acute (7 days)
and subacute (21 days) time points after rcTBI, reduction of the microglial
population did not alter the extent of axon injury as visualized by silver
staining. Further reduction of the microglial population by 56%, using an
intermediate dose (10 mg/mL), also did not alter the extent of silver staining,
amyloid precursor protein accumulation, neurofilament labeling, or axon injury
evident by electron microscopy at 7 days postinjury. Longer treatment of CD11b-TK
mice with intermediate dose and treatment for 14 days with high-dose (50 mg/mL)
valganciclovir were both found to be toxic in this injury model. Altogether, these
data are most consistent with the idea that microglia do not contribute to acute
axon degeneration after multiple concussive injuries. The possibility of longer-
term effects on axon structure or function cannot be ruled out. Nonetheless,
alternative strategies directly targeting injury to axons may be a more beneficial
approach to concussion treatment than targeting secondary processes of microglial-
driven inflammation.
AN - rayyan-553782333
AU - Bennett, R. E.
AU - Brody, D. L.
DO - 10.1089/neu.2013.3320
IS - 19
J2 - J Neurotrauma
KW - Animals
Brain Injuries/*immunology/*pathology
Diffuse Axonal Injury/*pathology
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Microglia/*immunology
Real-Time Polymerase Chain Reaction
Brain
Microglia
Brain Injuries
Axons
LA - eng
N1 - Department of Neurology, Washington University , St. Louis, Missouri.
PY - 2014
SP - 1647-63
ST - Acute reduction of microglia does not alter axonal injury in a mouse model of
repetitive concussive traumatic brain injury
T2 - Journal of neurotrauma
TI - Acute reduction of microglia does not alter axonal injury in a mouse model of
repetitive concussive traumatic brain injury
VL - 31
Y2 - 10 y3 - 1
ID - 10241
ER -
TY - JOUR
AB - So far, it was supposed that the increase of electrical impedance following
cochlear implant (CI) insertion was due to technical defects of the electrode,
inflammatory and/or formation of scar tissue along the electrode. However, it was
recently reported that corrosion of the platinum electrode contacts may be the
reason for high impedances. It could be shown that platinum particles were stripped
from the electrode surfaces. Its potential cytotoxic effects within the inner ear
remains to be examined. In this study in vitro cell culture models of the mouse
organ of Corti cell line (HEI-OC1) and the spiral ganglion (SG) cells derived from
the cochleae neonatal rats were used to investigate the effects of the
polyvinylpyrrolidone coated platinum nanoparticles (Pt-NPPVP, 3 nm) on cell
metabolism, neuronal survival and neurite outgrowth. Our data revealed no decrease
of the metabolic activity of the HEI-OC1 cells at Pt-NPPVP concentrations between
50-150 mu g/ml. Also, staining with Calcein AM/EthD demonstrated prevalent presence
of vital cells. As shown by transmission electron microscopy no Pt-NPPVP could be
found at the cell surface or in the cytosol of the HEI-OC1 cells. Similarly, the SG
cells exposed to 20-100 mu g/ml Pt-NPPVP did not show any reduced survival rate and
neurite outgrowth following staining of the neurofilament antigen even at the
highest Pt-NPPVP concentration. Although the SG cells were exposed to Pt-NPPVP for
further 72 h and 96 h immunocytochemical staining of the glial cells and
fibroblasts presented normal cell morphology and growth independently of the
cultivation period. Our data indicates that the used Pt-NPPVP do not trigger the
cellular uptake and, thus, presumable do not initiate apoptotic pathways in cells
of the organ of Corti cell line or the auditory nerve. The protection mechanisms to
the Pt-NPPVP interactions remain to be clarified.
AN - rayyan-553780206
AU - Berger, E.
AU - Brandes, G.
AU - Reifenrath, J.
AU - Lenarz, T.
AU - Durisin, M.
AU - Wissel, K.
DO - 10.1371/journal.pone.0284794
IS - 4
KW - Cells, Cultured
Ear, Inner
PY - 2023
SN - 1932-6203
ST - In vitro impact of platinum nanoparticles on inner ear related cell culture
models
T2 - PLOS ONE
TI - In vitro impact of platinum nanoparticles on inner ear related cell culture
models
VL - 18
Y2 - 4 y3 - 24
ID - 8241
ER -
TY - JOUR
AB - The present study focused on synthesizing, characterization, and evaluating
an in vitro anti-inflammatory and cytotoxic potential of green synthesized silver
nanoparticles (TB-Ag NPs) from aqueous stem bark extract of Terminalia brownii (TB-
AQ). The TB-Ag NPs were characterized by ultraviolet (UV)-visible, Fourier-
transform infrared spectroscopy (FTIR), dynamic light X-ray diffraction, and
energy-dispersive X-ray spectroscopy, as well as scanning and transmission electron
microscopy. The in vitro anti-inflammatory and cytotoxic potential of TB-Ag NPs and
TB-AQ were evaluated against RAW 264.7 macrophage and MDA-MB-231 triple-negative
breast cancer cells, respectively, by using 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assay. Further, the inhibitory effect on LPS-
induced production of inflammatory mediators PGE2 and NO in RAW 264.7 cell lines
was evaluated. The results showed that TB-Ag NPs were crystalline with face-
centered spherical polydispersed shaped nanoparticles with an average size between
20 and 67 ± 0.5 nm. Also, TB-Ag NPs had no cytotoxic effect on RAW cells (normal
healthy cells) in the range of 6.25–50 µg/mL. Besides, TB-Ag NPs at 50 µg/mL
concentration exhibited 67.02% of cytotoxic effect against MDA-MB-231 cells with
observed IC50 values of 29.08 µg/mL. TB-Ag NPs have shown significantly in
vitro anti-inflammatory activities by exhibiting dose-dependent NO and PGE2
inhibitory activities with IC50 values of 32.82 µg/mL and 67.25 µg/mL,
respectively. This study concluded that the novel green synthesized TB-Ag NPs can
be used as a potential novel anti-inflammatory and cytotoxicity agent to treat
inflammatory-related diseases and inflammatory breast cancer (invasive ductal
carcinoma) with biocompatible nature by targeting the tumor environment. © 2021,
The Author(s), under exclusive licence to Springer Science+Business Media, LLC,
part of Springer Nature.
AN - rayyan-553780207
AU - Berihu, H. T.
AU - Welderfael, T.
AU - Tekluu, B.
AU - Gopalakrishnan, V. K.
AU - Rao, M. R.
AU - Kumar, P. P. N. V.
AU - Shameem, U.
AU - Dogulas, P. J.
AU - Chaithanya, K. K.
DO - 10.1007/s12668-021-00885-8
IS - 4
MDA-MB-231breast cancer cell line
RAW macrophages
Terminalia brownii
Biocompatibility
Cell culture
Cells
Diseases
Energy dispersive spectroscopy
High resolution transmission electron microscopy
Metal nanoparticles
Scanning electron microscopy
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
arginine methyl ester
carbon dioxide
cardiac glycoside
deionized water
dimethyl sulfoxide
distilled water
flavonoid
formazan
gallic acid
lipopolysaccharide
nitric oxide
penicillin derivative
phenol
phytochemical
plant extract
prostaglandin E2
reduced nicotinamide adenine dinucleotide phosphate
silver nanoparticle
silver nitrate
sodium carbonate
streptomycin
sulfanilamide
tannin
toll like receptor
Energy dispersive X ray spectroscopy
Inflammatory breast cancers
Inflammatory mediators
Invasive ductal carcinomata
Raw 264.7 macrophages
Scanning and transmission electron microscopy
Triple-negative breast cancers
Article
bark
breast cancer
cell culture
cell density
cell viability
centrifugation
crystal
cytotoxicity
fetal bovine serum
incubation time
macrophage
MDA-MB-231 cell line
MTT assay
nonhuman
RAW 264.7 cell line
Soxhlet extraction
triple negative breast cancer
tumor microenvironment
ultraviolet radiation
X ray diffraction
zeta potential
Silver metallography
PY - 2021
SP - 998-1016
ST - Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver
Nanoparticles from Stem Bark of Terminalia brownii
T2 - BioNanoScience
TI - Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver
Nanoparticles from Stem Bark of Terminalia brownii
85113309220&doi=10.1007%2fs12668-021-00885-
8&partnerID=40&md5=7e28be98f8f6c29e49f726d49c549f59
VL - 11
ID - 8242
ER -
TY - JOUR
AB - Biofilms are matrices synthesized by bacteria containing polysaccharides,
DNA, and proteins. The development of biofilms in infectious processes can induce a
chronic inflammatory response that may progress to the destruction of tissues. The
treatment of biofilms is difficult because they serve as a bacterial mechanism of
defense and high doses of antibiotics are necessary to treat these infections with
limited positive results. It has been demonstrated that photothermal therapy using
gold nanorods (AuNRs) is an attractive treatment because of its anti-biofilm
activity. The purpose of this work was to generate a novel chitosan-based hydrogel
embedded with AuNRs to evaluate its anti-biofilm activity. AuNRs were synthesized
by the seed-mediated growth method and mixed with the chitosan-based hydrogel.
Hydrogels were characterized and tested against two bacterial strains by
irradiating the produced biofilm in the presence of the nanoformulation with a
laser adjusted at the near infrared spectrum. In addition, the safety of the
nanoformulation was assessed with normal human gingival fibroblasts. Results showed
that a significant bacterial killing was measured when biofilms were exposed to an
increase of 10 degrees C for a short time of 2 min. Moreover, no cytotoxicity was
measured when normal gingival fibroblasts were exposed to the nanoformulation using
the bactericidal conditions. The development of the reported formulation can be
used as a direct application to treat periodontal diseases or biofilm-produced
bacteria that colonize the oral cavity.
AN - rayyan-553780208
AU - Bermudez-Jimenez, C.
AU - Nino-Martinez, N.
AU - Patino-Marin, N.
AU - Martinez-Gutierrez, F.
AU - Ruiz, F.
AU - Bach, H.
AU - Martinez-Castanon, G.
DO - 10.1002/jbm.b.34392
IS - 2
KW - Hydrogel
Hydrogels
PY - 2020
SN - 1552-4973 1552-4981
SP - 333-342
ST - Effective control of biofilms by photothermal therapy using a gold nanorod
hydrogel
T2 - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
TI - Effective control of biofilms by photothermal therapy using a gold nanorod
hydrogel
VL - 108
Y2 - 2
ID - 8243
ER -
TY - COMP
AB - AIMS: To analyse the type and degree of inflammatory reaction initiated by
four sealers. METHODOLOGY: Twenty-four root canals of Macaca mulatta monkeys were
filled within the canal and eight were overfilled with AH26, Apexit, Endomethasone
or Grossman's sealers. The result of the treatment was evaluated after 6 months by
histological assessment of the periapical tissues. RESULTS: In the group of root
canals filled within the root, no inflammatory reaction was detected in specimens
of Apexit and Grossman's sealers, but the other two sealers initiated different
degrees of lymphocytic/plasmocytic tissue reactions. Endomethasone initiated a mild
lymphocytic/plasmocytic reaction in three of the nine cases and AH26 caused mild
lymphocytic/plasmocytic infiltration in two of the seven cases. In the group of
overfilled root canals, all four sealers initiated inflammatory reactions. The
periapical tissue reactions of overfilled root canals were similar to reactions
detected in cases filled within the canal. However, additional histological
features developed in specimens of Endomethasone and AH26: Endomethasone initiated
a foreign body-type granulomatous reaction around the sealer particles and AH26
particles were engulfed by macrophages. The overfilled root canals of Apexit and
Grossman's sealers initiated only lymphocytic/plasmocytic reactions. CONCLUSIONS:
This study suggests that sealers with different chemical compositions initiate
different histological reactions. It also emphasizes the importance of confining
root filling to the canal system because all sealers initiate inflammatory
reactions when they are present in the apical tissues
AN - rayyan-553782180
AU - Bernáth, M.
AU - Szabó, J.
CY - England
DO - 10.1046/j.1365-2591.2003.00662.x
ET - 4
J2 - Int Endod J
KW - *Administration, Topical
Animals
Anti-Inflammatory Agents/toxicity
Bismuth/toxicity
Calcium Hydroxide/*toxicity
Dexamethasone/toxicity
Drug Combinations
Epoxy Resins/toxicity
Extravasation of Diagnostic and Therapeutic Materials/complications
Formaldehyde/toxicity
*Hydrocortisone
Macaca mulatta
Periapical Periodontitis/*chemically induced
Root Canal Filling Materials/*toxicity
Root Canal Obturation/adverse effects
Silver/toxicity
Thymol/*analogs & derivatives/toxicity
Titanium/toxicity
Zinc Oxide-Eugenol Cement/toxicity
LA - eng
N1 - Department of Dentistry and Oral Surgery, Faculty of Medicine, Pécs
University, Pécs, Hungary. bernath@konfog.sote.hu
PY - 2003
SN - 0143-2885 (Print)
SP - 256-61
ST - Tissue reaction initiated by different sealers
T2 - International endodontic journal
TI - Tissue reaction initiated by different sealers
VL - 36
Y2 - 4
ID - 10090
ER -
TY - JOUR
AB - The biological effects of acute particulate air pollution exposure in host
innate immunity remain obscure and have relied largely on in vitro models. We
hypothesized that single acute exposure to ambient or engineered particulate matter
(PM) in the absence of other secondary stimuli would activate lung dendritic cells
(DC) in vivo and provide information on the early immunological events of PM
exposure and DC activation in a mouse model naïve to prior PM exposure. Activation
of purified lung DC was studied following oropharyngeal instillation of ambient
particulate matter (APM). We compared the effects of APM exposure with that of
diesel-enriched PM (DEP), carbon black particles (CBP) and silver nanoparticles
(AgP). We found that PM species induced variable cellular infiltration in the lungs
and only APM exposure induced eosinophilic infiltration. Both APM and DEP activated
pulmonary DC and promoted a Th2-type cytokine response from naïve CD4+ T cells ex
vivo. Cultures of primary peribronchial lymph node cells from mice exposed to APM
and DEP also displayed a Th2-type immune response ex vivo. We conclude that
exposure of the lower airway to various PM species induces differential
immunological responses and immunomodulation of DC subsets. Environmental APM and
DEP activated DC in vivo and provoked a Th2 response ex vivo. By contrast, CBP and
AgP induced altered lung tissue barrier integrity but failed to stimulate CD4+ T
cells as effectively. Our work suggests that respirable pollutants activate the
innate immune response with enhanced DC activation, pulmonary inflammation and Th2-
immune responsiveness. Copyright © 2010 S. Karger AG.
AN - rayyan-553780209
AU - Bezemer, G. F. G.
AU - Bauer, S. M.
AU - Oberdörster, G.
AU - Breysse, P. N.
AU - Pieters, R. H. H.
AU - Georas, S. N.
AU - Williams, M. A.
DO - 10.1159/000321725
IS - 2
KW - Allergic immunity
Dendritic cell
Immunotoxicology
Inflammation
Innate immunity
Lung
Nanoparticles
Particulate matter
Toxicology
Air Pollutants
Animals
Carbon
Dendritic Cells
Immunity, Innate
Mice
Mice, Inbred C57BL
Particulate Matter
Pneumonia
Silver
Th2 Cells
Vehicle Emissions
carbon
diesel fuel
gamma interferon
interleukin 10
interleukin 4
interleukin 5
interleukin 6
silver nanoparticle
ambient air
animal cell
animal experiment
article
CD4+ T lymphocyte
cell activation
cell infiltration
cell interaction
cell viability
cytokine production
cytotoxicity
dendritic cell
environmental exposure
eosinophil
ex vivo study
exhaust gas
immunomodulation
in vivo study
innate immunity
lung alveolus cell type 2
lymph node cell
macrophage activation
mouse
nonhuman
particulate matter
peribronchial lymph node cell
priority journal
protein secretion
T lymphocyte subpopulation
PY - 2011
SP - 150-166
ST - Activation of pulmonary dendritic cells and Th2-type inflammatory responses
on instillation of engineered, environmental diesel emission source or ambient air
pollutant particles in vivo
T2 - Journal of Innate Immunity
TI - Activation of pulmonary dendritic cells and Th2-type inflammatory responses
on instillation of engineered, environmental diesel emission source or ambient air
pollutant particles in vivo
79951993777&doi=10.1159%2f000321725&partnerID=40&md5=abd78ffde6bf101e45d7a91a828a1b
2d
VL - 3
ID - 8244
ER -
TY - JOUR
AB - The aim of this study was to evaluate radiographically and histologically the
pulpal and periapical response to self-adhesive (Rely X (TM) Unicem) and self-
etching and self-curing (Multilink (R)) resin-based luting materials in deep
cavities in dogs' teeth. Deep class V cavities (0.5-mm-thick dentin) were prepared
in 60 canine premolars and the following materials were applied on cavity floor:
Groups I/V-RelyX (TM) Unicem; Groups II/VI-Multilink (R); Groups III/VII-zinc
phosphate cement (control) and; Groups IV/VIII-gutta-percha (control). Cavities
were restored with silver amalgam. Animals were euthanized after 10 days (groups I-
IV) and 90 days (groups V-VIII). Tooth/bone blocks were radiographed and processed
for histopathological evaluation of pulp and periapical tissue response to the
materials. All materials presented similar histopathological features and
radiographic findings at both periods. The pulp tissue was intact. The apical and
periapical regions and periodontal ligament thickness were normal. No inflammatory
cells, resorption of mineralized tissue (dentin, cementum, and alveolar bone) or
bacteria were observed. The lamina dura was intact and no areas of periapical bone
rarefaction or internal/external root resorption were observed radiographically. It
can be concluded that Rely X (TM) Unicem and Multilink (R) caused no adverse tissue
reactions and may be indicated for cementation of indirect restorations in deep
dentin cavities without pulp exposure. (C) 2015 Wiley Periodicals, Inc.
AN - rayyan-553780210
AU - Bezzon, O. L.
AU - Rivera, D. S. H.
AU - Silva, R. A. B.
AU - Oliveira, D. S. B.
AU - Silva-Herzog, D.
AU - Nelson, P.
AU - Lucisano, M. P.
AU - Silva, L. A. B.
DO - 10.1002/jemt.22590
IS - 12
KW - Dogs
PY - 2015
SN - 1059-910X 1097-0029
SP - 1098-1103
ST - Resin Luting Materials: Tissue Response in Dog's Teeth
T2 - MICROSCOPY RESEARCH AND TECHNIQUE
TI - Resin Luting Materials: Tissue Response in Dog's Teeth
VL - 78
Y2 - 12
ID - 8245
ER -
TY - JOUR
AB - The increase in drug-resistant strains of Staphylococcus aureus, especially
methicillin-resistant S. aureus (MRSA), has led to an increased rate of infection-
related mortality. The emergence of drug resistance has rendered many antibiotics
ineffective. The poor penetration and retention of antibiotics in mammalian cells
lead to recurrent latent infections. Thus, there is an increasing need for
biodegradable, non-toxic anti-infectives that are effective in treating MRSA
infections. Phytochemicals such as berberine (BBR) and curcumin (CCR) have long
been explored for their antibacterial activities, but their efficacy is often
limited due to low bioavailability, water solubility, and poor cell penetration.
When used in combination these antimicrobials did not show any synergistic effect
against MRSA. Here, both of them were co-encapsulated in liposomes (BCL) and
evaluated for biocompatibility, synergistic antimicrobial activity, intracellular
infections, associated inflammation, and on biofilms formed by MRSA. Co-
encapsulation of BBR and CCR in liposomes decreased their MICs by 87% and 96%,
respectively, as compared to their free forms with a FICI of 0.13, indicating
synergy between them. BCL inhibited the growth of MRSA and prevented biofilm
formation better than free drugs. Co-culture studies showed that intracellular
infection was reduced to 77% post BCL treatment. It also reduced the production of
pro-inflammatory cytokines by macrophages following infection. The liposomes were
found to be five times more efficient than clindamycin and can be used as a
potential antimicrobial carrier against intracellular infections.
AN - rayyan-553780213
AU - Bhatia, E.
AU - Sharma, S.
AU - Jadhav, K.
AU - Banerjee, R.
DO - 10.1039/d0tb02036b
IS - 3
KW - Liposomes
Staphylococcus
Inflammation
PY - 2021
SN - 2050-750X 2050-7518
SP - 864-875
ST - Combinatorial liposomes of berberine and curcumin inhibit biofilm formation
and intracellular methicillin resistant Staphylococcus aureus infections and
associated inflammation
T2 - JOURNAL OF MATERIALS CHEMISTRY B
TI - Combinatorial liposomes of berberine and curcumin inhibit biofilm formation
and intracellular methicillin resistant Staphylococcus aureus infections and
associated inflammation
VL - 9
Y2 - 1 y3 - 21
ID - 8248
ER -
TY - JOUR
AB - Engineered nanomaterials (ENMs) are being produced for an increasing number
of applications. Therefore, it is important to assess and categorize ENMs on the
basis of their hazard potential. The immune system is the foremost defence against
foreign bodies. Here we performed cytokine profiling of a panel of nineteen
representative ENMs procured from the Joint Research Centre (JRC) and commercial
sources. Physicochemical characterization was performed using dynamic light
scattering. The ENMs were all shown to be endotoxin content free. The human
macrophage-differentiated THP.1 cell line was employed for cytotoxicity screening
and based on the calculated IC50 values, the multi-walled carbon nanotubes
(MWCNTs), ZnO, Ag and SiO2 NMs were found to be the most cytotoxic while single-
walled carbon nanotubes (SWCNTs), TiO2, BaSO4 and CeO2 NMs, as well as the
nanocellulose materials, were non-cytotoxic (at doses up to 100 µg/mL). Multiplex
profiling of cytokine and chemokine secretion indicated that the TiO2, SiO2, BaSO4,
CeO2 and nanocellulose materials induced potent inflammatory responses at sub-
cytotoxic doses. Hierarchical clustering of cytokine responses coupled with pathway
analysis demonstrated that the panel of ENMs could be segregated into two distinct
groups characterized by activation and deactivation, respectively, of PPAR
(peroxisome proliferator-activated receptor)/LXR (liver X receptor/retinoid X
receptor) nuclear receptor pathways (NRPs). Furthermore, using rosiglitazone, a
selective PPAR-γ agonist, we could show that PPAR-γ played an important role in the
activation of inflammatory responses in cells exposed to TiO2 and SiO2 NMs. These
studies show that ENMs of diverse chemical compositions can be grouped according to
their inflammatory potential. © 2017 The Author(s). Published by Informa UK
Limited, trading as Taylor & Francis Group.
AN - rayyan-553780214
AU - Bhattacharya, K.
AU - Kiliç, G.
AU - Costa, P. M.
AU - Fadeel, B.
DO - 10.1080/17435390.2017.1363309
IS - 6
KW - cytotoxicity
hierarchical clustering
in vitro screening
inflammation
Nanomaterials
Cell Line
Cell Survival
Cluster Analysis
Cytokines
Hazardous Substances
Humans
Macrophages
Nanostructures
Nanotubes, Carbon
Particle Size
cell nucleus receptor
cerium oxide nanoparticle
chemokine
cytokine
endotoxin
liver X receptor
multi walled nanotube
nanomaterial
peroxisome proliferator activated receptor
retinoid X receptor
rosiglitazone
silica nanoparticle
silver nanoparticle
single walled nanotube
carbon nanotube
Article
cytokine release
cytokine response
cytotoxicity test
human
human cell
IC50
in vitro study
macrophage
particle size
physical chemistry
priority journal
risk assessment
zeta potential
cell line
cell survival
chemistry
classification
cluster analysis
dangerous goods
dose response
drug effects
immunology
toxicity
Mass Screening
PY - 2017
SP - 809-826
ST - Cytotoxicity screening and cytokine profiling of nineteen nanomaterials
enables hazard ranking and grouping based on inflammogenic potential
T2 - Nanotoxicology
TI - Cytotoxicity screening and cytokine profiling of nineteen nanomaterials
enables hazard ranking and grouping based on inflammogenic potential
85027882619&doi=10.1080%2f17435390.2017.1363309&partnerID=40&md5=18a8487e3d6de498f0
35e46bcd6bd51c
VL - 11
ID - 8249
ER -
TY - COMP
AB - BACKGROUND: Visceral leishmaniasis (VL) caused by the protozoan parasite
Leishmania donovani causes severe disease. Age appears to be critical in
determining the clinical outcome of VL and at present there is no effective vaccine
available against VL for any age group. Previously, we showed that genetically
modified live attenuated L. donovani parasites (LdCen-/-) induced a strong
protective innate and adaptive immune response in young mice. In this study we
analyzed LdCen-/- parasite mediated modulation of innate and adaptive immune
response in aged mice (18 months) and compared to young (2 months) mice.
METHODOLOGY: Analysis of innate immune response in bone marrow derived dendritic
cells (BMDCs) from both young and aged mice upon infection with LdCen-/- parasites,
showed significant enhancement of innate effector responses, which consequently
augmented CD4+ Th1 cell effector function compared to LdWT infected BMDCs in vitro.
Similarly, parasitized splenic dendritic cells from LdCen-/- infected young and
aged mice also revealed induction of proinflammatory cytokines (IL-12, IL-6, IFN-γ
and TNF) and subsequent down regulation of anti-inflammatory cytokine (IL-10) genes
compared to LdWT infected mice. We also evaluated in vivo protection of the
LdCen-/- immunized young and aged mice against virulent L. donovani challenge.
Immunization with LdCen-/- induced higher IgG2a antibodies, lymphoproliferative
response, pro- and anti-inflammatory cytokine responses and stimulated splenocytes
for heightened leishmanicidal activity associated with nitric oxide production in
young and aged mice. Furthermore, upon virulent L. donovani challenge, LdCen-/-
immunized mice from both age groups displayed multifunctional Th1-type CD4 and
cytotoxic CD8 T cells correlating to a significantly reduced parasite burden in the
spleen and liver compared to naïve mice. It is interesting to note that even though
there was no difference in the LdCen-/- induced innate response in dendritic cells
between aged and young mice; the adaptive response specifically in terms of T cell
and B cell activation in aged animals was reduced compared to young mice which
correlated with less protection in old mice compared to young mice. CONCLUSIONS:
Taken together, LdCen-/- immunization induced a significant but diminished host
protective response in aged mice after challenge with virulent L. donovani
parasites compared to young mice.
AN - rayyan-553782158
AU - Bhattacharya, P.
AU - Dey, R.
AU - Dagur, P. K.
AU - Joshi, A. B.
AU - Ismail, N.
AU - Gannavaram, S.
AU - Debrabant, A.
AU - Akue, A. D.
AU - KuKuruga, M. A.
AU - Selvapandiyan, A.
AU - McCoy, J. P., Jr.
AU - Nakhasi, H. L.
CY - United States
DO - 10.1371/journal.pntd.0004963
ET - 8
J2 - PLoS Negl Trop Dis
KW - Adaptive Immunity
Aging/*immunology
Animals
Antibodies, Protozoan/blood
Cells, Cultured
Coculture Techniques
Cytokines/immunology
Dendritic Cells/immunology
Female
Gene Knockout Techniques
Immunity, Innate
Leishmania donovani/genetics/*immunology
Leishmaniasis Vaccines/*therapeutic use
Leishmaniasis, Visceral/*immunology/parasitology
Macrophages/immunology
Mice
Mice, Inbred BALB C
Th1 Cells/*immunology
LA - eng
N1 - Division of Emerging and Transfusion Transmitted Disease, Center for
Biologics Evaluation and Research, Food and Drug Administration, Silver Spring,
Maryland, United States of America.; Division of Emerging and Transfusion
Transmitted Disease, Center for Biologics Evaluation and Research, Food and Drug
Administration, Silver Spring, Maryland, United States of America.; Flow Cytometry
Core, National Heart, Lung, and Blood Institute, National Institutes of Health,
Bethesda, Maryland, United States of America.; Division of Emerging and Transfusion
Administration, Silver Spring, Maryland, United States of America.; Division of
Emerging and Transfusion Transmitted Disease, Center for Biologics Evaluation and
Research, Food and Drug Administration, Silver Spring, Maryland, United States of
America.; Division of Emerging and Transfusion Transmitted Disease, Center for
Maryland, United States of America.; Division of Emerging and Transfusion
Administration, Silver Spring, Maryland, United States of America.; Office of
Vaccines Research and Review, Center for Biologics Evaluation and Research, Silver
Spring, Maryland, United States of America.; Office of Vaccines Research and
Review, Center for Biologics Evaluation and Research, Silver Spring, Maryland,
United States of America.; Institute of Molecular Medicine, New Delhi, India.; Flow
Cytometry Core, National Heart, Lung, and Blood Institute, National Institutes of
Health, Bethesda, Maryland, United States of America.; Division of Emerging and
Transfusion Transmitted Disease, Center for Biologics Evaluation and Research, Food
and Drug Administration, Silver Spring, Maryland, United States of America.
PY - 2016
SP - e0004963
ST - Live Attenuated Leishmania donovani Centrin Knock Out Parasites Generate Non-
inferior Protective Immune Response in Aged Mice against Visceral Leishmaniasis
T2 - PLoS neglected tropical diseases
TI - Live Attenuated Leishmania donovani Centrin Knock Out Parasites Generate Non-
inferior Protective Immune Response in Aged Mice against Visceral Leishmaniasis
VL - 10
Y2 - 8
ID - 10069
ER -
TY - JOUR
AB - Nanocrystalline silver (NPI 32101) has been demonstrated to have
antimicrobial and anti-inflammatory properties. The purpose of this study was to
assess the effect of NPI 32101 in a rat model of ulcerative colitis and the
possible mechanisms of action of the effects observed. NPI 32101, 4 mg/kg
intracolonically or 40 mg/kg orally, significantly reduced colonic inflammation
compared to the placebo and no-treatment groups. Sulfasalazine (100 mg/kg), either
intracolonically or orally, also reduced colonic inflammation. NPI 32101
significantly suppressed the expression of matrix metalloproteinase (MMP)-9, tumor
necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-12, whereas
sulfasalazine suppressed MMP-9, IL-1beta, and TNF-alpha, but not IL-12, compared to
placebo. MMP-9 activity was reduced by NPI 32101 and sulfasalazine. NPI 32101
administered intracolonically or orally decreases ulcerative colitis in a rat model
and is as effective as sulfasalazine. NPI 32101 treatment suppresses the expression
and activity of MMP-9 and the expression of TNF-alpha, IL-1beta, and IL-12,
mechanisms by which NPI 32101 may exert its anti-inflammatory effects. NPI 32101
may have therapeutic potential for treatment of ulcerative colitis.
AN - rayyan-553782133
AU - Bhol, K. C.
AU - Schechter, P. J.
DO - 10.1007/s10620-006-9738-4
IS - 10
J2 - Dig Dis Sci
KW - Animals
Benzenesulfonates/toxicity
Colitis, Ulcerative/chemically induced/*drug therapy/pathology
Colon
Drug Administration Routes
Follow-Up Studies
Haptens
Intestinal Mucosa/drug effects/metabolism/pathology
Male
Matrix Metalloproteinase 2/metabolism
Matrix Metalloproteinase 9/metabolism
Metal Nanoparticles/administration & dosage/*therapeutic use
Rats
Severity of Illness Index
Silver/administration & dosage/*therapeutic use
Treatment Outcome
Colitis
Colitis, Ulcerative
LA - eng
N1 - NUCRYST Pharmaceuticals Inc., 50 Audubon Road, Wakefield, MA 01880, USA.
Kbhol@nucryst.com
PY - 2007
SN - 0163-2116 (Print)
SP - 2732-42
ST - Effects of nanocrystalline silver (NPI 32101) in a rat model of ulcerative
colitis
T2 - Digestive diseases and sciences
TI - Effects of nanocrystalline silver (NPI 32101) in a rat model of ulcerative
colitis
VL - 52
Y2 - 10
ID - 10044
ER -
TY - JOUR
AB - The recent need for remote health wellness monitoring has led to the
extensive use of wearable sensors. Owing to their increased use, these sensors are
required to exhibit both functionality and safety to the user. A major component in
the fabrication of these sensors and their associated circuitry is the use of
metallic/organic conductive inks. However, very less is known about the interfacial
and molecular interactions of these inks with biological matter as they can result
in an inflammatory reaction to the user. Significant efforts are thus needed to
explore and improve the bio-acceptability of such conductive ink-based wearable
sensors. The present study investigates the biocompatibility of encapsulated and
non-encapsulated wearable electrochemical sensors used for sensing uric acid as a
biomarker for wound healing fabricated using screen-printing technique. Ionic
release of metallic ions was investigated first to understand the susceptibility of
the conductive inks towards ionic leaching when in contact with a fluid. Time-lapse
investigation using ICPS (inductive couple plasma spectroscopy) shows a high
concentration (607.31 ppb) of leached silver (Ag+) ions from the non-encapsulated
sensors. The cell viability data suggests a 2.5-fold improvement in the sensor
biocompatibility for an encapsulated sensor. While the carbon ink shows negligible
effect on cell viability, the silver ink elicits significant decrease (< 50%) in
cell viability at concentrations higher than 2 mg ml-1. The toxicity pathway of
these sensors was further determined to be through the generation of reactive
oxygen species resulting in over 20% apoptotic cell death. Our results show that
the lower biocompatibility of the non-encapsulated sensor attributes to the higher
leaching of Ag+ ions from the printed inks which elicits several different
inflammatory pathways. This work highlights the importance biocompatibility
evaluation of the material used in sensor fabrication to develop safe and
sustainable sensors for long-term applications. © 2022, This is a U.S. Government
work and not under copyright protection in the US; foreign copyright protection may
apply.
AN - rayyan-553780217
AU - Bhushan, P.
AU - Kamat, V.
AU - Abrol, I.
AU - Kaushik, A.
AU - Bhansali, S.
DO - 10.1038/s41598-022-13810-0
IS - 1
KW - Humans
Inflammation
Ink
Ions
Silver
Wearable Electronic Devices
ink
ion
silver
electronic device
human
inflammation
Tocopherols
PY - 2022
ST - Bio-acceptability of wearable sensors: a mechanistic study towards evaluating
ionic leaching induced cellular inflammation
T2 - Scientific Reports
TI - Bio-acceptability of wearable sensors: a mechanistic study towards evaluating
ionic leaching induced cellular inflammation
85132938484&doi=10.1038%2fs41598-022-13810-
0&partnerID=40&md5=8f745c6cda74cc9ade4d599b3ed83174
VL - 12
ID - 8252
ER -
TY - JOUR
AB - 24 hours after administration of Intration in toxic doses, necrosis takes
place with calcifications of single fibres, focal acidophilic degeneration, oedema
of stroma and glycogen loss in the muscles of ventricles and auricles of the heart.
The necrosis foci were sometimes accompanied by an inflammatory reaction. In the
heart valves the oedema of stroma, subendothelial inflammatory infiltrations, as
well as degeneration and desquamation of endothelium cells were found. After 8 days
a significant decrease of the extent of foci of acidophilic degeneration occurred.
In the existing foci, however, coagulation necrosis of the cardiac muscle fibres
was evident. In the valves, besides oedema, the intensification of the inflammatory
reaction and focal fibrousness, as well as endothelium regeneration were found.
After PAM administration, necrosis and calcification of fibres were not found;
there were, however, extensive foci of acidophilic degeneration of cardiac muscle
fibers and more numerous inflammatory infiltrations in the valves. After 8 days,
the cardiac muscle fibres in the state of coagulation necrosis were more numerous
than in the untreated animals. Oedema and the increase of capillary vessel
endothelia, as well as ischaemia of cardiac muscle were also observed. The authors
discuss the possibility of the complex action of Intration, through the stimulation
of the parasympathetic system, as well as directly upon the cell. PAM abolishes
probably only the pharmacological action of Intration, but provokes intensification
of secondary changes, which could follow the action of the products of
decomposition of Intration and of PAM alike.
AN - rayyan-553780218
AU - Biernat, S.
AU - Giermaziak, H.
IS - 4
KW - intration
methenamine periodate silver
pam
thiometon
unclassified drug
dose response
drug toxicity
heart
histology
in vitro study
intoxication
intravenous drug administration
rat
theoretical study
Rats
Melphalan
PY - 1976
SP - 241-251
ST - Pathomorphological changes in rat heart after administration of intration in
toxic doses and after large doses of PAM (Polish)
T2 - Medycyna Pracy
TI - Pathomorphological changes in rat heart after administration of intration in
toxic doses and after large doses of PAM (Polish)
0017139964&partnerID=40&md5=38f40b7a526341e0809f1b1562db0a85
VL - 27
ID - 8253
ER -
TY - JOUR
AB - SUMMARY: Liver transplantation is the only available method to treat liver
failure induced by chronic liver injury. We sought to determine whether the
angiotensin-converting enzyme inhibitor, captopril, can inhibit the development of
chronic liver injury induced by the hepatotoxic agent thioacetamide (TAA) in
association with the suppression of inflammation (hsCRP, TNF-α, and IL-6) /
hypoxia- inducible factor 1-alpha (HIF-1α) / profibrosis (TIMP-1, MMP-9, and α-SMA)
axis that mediates liver injury. Therefore, the model group of rats was injected
for eight weeks with 200 mg/kg TAA starting at week two. The protective group was
pretreated with 150 mg/ kg captopril daily for two weeks prior to TAA injections
and continued receiving both capropril and TAA agents until being humanely
scrificed at week 10. We observed a substantial damage to liver tissue in the model
group as demonstrated by a significant (p<0.0001) increase in blood and hepatic
tissue levels of high sensitivity C-reactive protein (hsCRP), tumor necrosis
factor-a (TNF-α), interleukin- 6 (L-6), HIF-1α, tissue inhibitor of
metalloproteinases-1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), alpha-smooth
muscle actin (α-SMA), alanine aminotransferase (ALT), and aspartate
aminotransferase (AST). All these parameters were significantly (p<0.0244)
protected by captopril. Also, a significant (p<0.0001) positive correlation was
observed between a-SMA (profibrosis) and the serum and tissue levels of hsCRP, TNF-
α, HIF-1α, TIMP-1, MMP-9, and ALT. Thus, these findings suggest that the induction
of chronic liver injury by the hepatotoxic compound, TAA is associated with the
upregulation of inflammation/HIF-1α/profibrosis, with captopril exhibiting
beneficial hepatic pleotropic effects. El trasplante de hígado es el único método
disponible para tratar la insuficiencia hepática inducida por una lesión hepática
crónica. Buscamos determinar si el inhibidor de la enzima convertidora de
angiotensina, captopril, puede inhibir el desarrollo de lesión hepática crónica
inducida por el agente hepatotóxico tioacetamida (TAA) en asociación con la
supresión de la inflamación (hsCRP, TNF-α e IL-6) / factor inducible por hipoxia 1-
alfa (HIF-1α) / profibrosis (TIMP-1, MMP-9 y α- SMA) eje que media la lesión
hepática. Por lo tanto, al grupo modelo de ratas se le inyectó durante ocho semanas
200 mg/kg de TAA a partir de la semana dos. El grupo protector fue pretratado con
150 mg/kg de captopril al día durante dos semanas antes de las inyecciones de TAA y
continuó recibiendo capropril y agentes TAA hasta que fue sacrificado en la semana
10. Observamos un daño sustancial en el tejido hepático en el grupo modelo, como lo
demuestra un aumento significativo (p<0,0001) de los niveles en sangre y tejido
hepático de proteína C reactiva de alta sensibilidad (hsCRP), factor de necrosis
tumoral-α (TNF-a), interleucina-6 (L-6), HIF-1α, inhibidor tisular de
metaloproteinasas-1 (TIMP-1), metaloproteinasa de matriz-9 (MMP-9), actina de
músculo liso alfa (α-SMA), alanina aminotransferasa (ALT) y aspartato
aminotransferasa (AST). Todos estos parámetros estaban significativamente
(p<0,0244) protegidos por captopril. Además, se observó una correlación positiva
significativa (p<0,0001) entre α-SMA (profibrosis) y los niveles séricos y
tisulares de hsCRP, TNF-α, HIF-1α, TIMP- 1, MMP-9 y ALT. Por lo tanto, estos
hallazgos sugieren que la inducción de daño hepático crónico por el compuesto
hepatotóxico, TAA, está asociada con la regulación al alza de la inflamación/HIF-
1α/profibrosis, con captopril exhibiendo efectos pleotrópicos hepáticos
beneficiosos.
AN - rayyan-553780219
AU - Bin-Jaliah, Ismaeel
DO - 10.4067/S0717-95022023000200362
IS - 2
KW - HIF-1alpha
Inflamación
Inflammation
Lesión hepática
Liver injury
Model
Modelo
Profibrosis
Rat
Rata
Thioacetamide
Tioacetamida
Captopril
Rats
LA - en
PY - 2023
SN - 0717-9367
SP - 362-367
ST - Captopril inhibits thioacetamide-induced chronic liver injury associated with
the suppression of inflammation / hypoxia- inducible factor 1-alpha / profibrogenic
axis-mediated hepatotoxicity in rats
TI - Captopril inhibits thioacetamide-induced chronic liver injury associated with
the suppression of inflammation / hypoxia- inducible factor 1-alpha / profibrogenic
axis-mediated hepatotoxicity in rats
VL - 41
Y2 - 4
ID - 8254
ER -
TY - JOUR
AB - A proof of concept for designing multi-drug-delivery systems suitable for
self-drug-delivery is disclosed. Simple coordination chemistry was employed to
anchor two kinds of drugs namely isoniazid (IZ - anti-tuberculosis), various non-
steroidal-anti-inflammatory-drugs (NSAIDs) namely ibuprofen-IBU, fenoprofen-FEN,
naproxen-NAP, diclofenac-DIC and mefenamic acid-MEF and Zn(NO3)(2) to synthesize a
series of 1D coordination polymers namely IZIBU, IZFEN, IZNAP, IZDIC and IZMEF
which were structurally characterized by single crystal X-ray diffraction (SXRD).
The coordination polymers wherein both types of drugs were anchored to Zn(II) metal
centers could easily be ground to nano-sized particles suitable for biological
studies by hand grinding in a mortar and pestle. Zone inhibition studies revealed
that all the coordination polymers possessed antibacterial properties against Gram
positive, Gram negative and mycobacteria namely Mycobacterium tuberculosis (M.tb).
Detailed studies carried out on IZDIC employing flow cytometry and confocal
microscopy under various staining conditions established that such antibacterial
activity was due to the generation of reactive oxygen species (ROS) such as nitric
oxide (NO) and also inhibition of mycolic acid leading to incomplete cell wall
formation. It was also established that IZDIC could indeed inhibit the growth of
M.tb within a mouse macrophage host cell namely RAW 264.7 thereby simulating the
treatment of Tuberculosis (TB) under in vitro conditions. Scratch assay and cell
cycle analysis on a human lung cancer cell line (A549) revealed its anti-cancer
property, thereby indicating its potential as a multidrug-delivery system. In vivo
toxicity assessment (serum parameters, histopathology, and haemolysis) carried out
on BALB/c mice showed that IZDIC was safe up to a concentration of 100 mg kg(-1).
Finally, a reasonably high yield in bulk synthesis, stability under high
temperature and humid conditions, tabletability and, slow and sustained release of
the drug component of IZDIC suggested its suitability in real-life applications as
multi-drug-delivery systems.
AN - rayyan-553780220
AU - Biswas, P.
AU - Datta, H. K.
AU - Dastidar, P.
DO - 10.1039/d2bm00752e
IS - 21
KW - Drug Delivery Systems
Polymerization
Polymers
Tuberculosis
PY - 2022
SN - 2047-4830 2047-4849
SP - 6201-6216
ST - Designing coordination polymers as multi-drug-self-delivery systems for
tuberculosis and cancer therapy: in vitro viability and in vivo toxicity assessment
T2 - BIOMATERIALS SCIENCE
TI - Designing coordination polymers as multi-drug-self-delivery systems for
tuberculosis and cancer therapy: in vitro viability and in vivo toxicity assessment
VL - 10
Y2 - 10 y3 - 25
ID - 8255
ER -
TY - JOUR
AB - Pellets of a glass ionomer-silver cement and a zinc oxide-eugenol cement were
implanted into the soft tissues and bones of 30 rats. Following experimental
periods of 14, 30, and 80 days, the animals were killed and tissue sections were
prepared. The responses to each of the materials initially and at 30 days consisted
of mild inflammation. No severe inflammatory responses were noted in any of the
groups. By 80 days, although mild inflammation persisted, the materials appeared to
be well tolerated. Bone apposition occurred in the glass ionomer-silver cement
group; the zinc oxide-eugenol group produced fibrosis.
AN - rayyan-553782145
AU - Blackman, R.
AU - Gross, M.
AU - Seltzer, S.
DO - 10.1016/s0099-2399(89)80112-8
IS - 2
J2 - J Endod
KW - Animals
Bone and Bones/drug effects
Cermet Cements
Dental Alloys/toxicity
Dental Cements/*toxicity
Glass Ionomer Cements/*toxicity
Rats
Rats, Inbred Strains
Silver/toxicity
Connective Tissue
LA - eng
PY - 1989
SN - 0099-2399 (Print)
SP - 76-9
ST - An evaluation of the biocompatibility of a glass ionomer-silver cement in rat
connective tissue
T2 - Journal of endodontics
TI - An evaluation of the biocompatibility of a glass ionomer-silver cement in rat
connective tissue
VL - 15
Y2 - 2
ID - 10056
ER -
TY - JOUR
AB - Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in the
treatment of rheumatoid arthritis and osteoarthritis, and are also indicated for
periarticular and musculoskeletal diseases. However, the use of NSAIDs is limited
by their toxicity. NSAIDs have a variable effect on the regeneration of cells and
extracellular matrix that depends on the dose used. In this work, we examined the
effect of naproxen, a NSAID, on tail fin regeneration in carp (Cyprinus carpio), a
teleost fish that is a good model for studying the growth of connective tissue in
vivo. We used histochemical, ultrastructural and morphometric analyses to assess
the synthesis, deposition and organization of the lepidotrichial extracellular
matrix components and the total area of regenerating fins, including lepidotrichia,
epidermis and connective tissue. Naproxen (15.6 mg/L in the tank water) did not
affect the formation of the epidermal cap and blastema, the differentiation of
blastemal cells in scleroblasts or the synthesis, deposition, organization and
mineralization of lepidotrichial matrix components. In addition, there was no
significant difference in the area of regenerated tissue between control and
naproxen-treated fishes. These results indicate that at the concentration tested,
naproxen had no effect on tail fin regeneration.
AN - rayyan-553780222
AU - Bõckelmann, Petra Karla
AU - Bechara, Ivanira José
IS - 1
KW - Naproxen
Regeneration
LA - en
PY - 2007
SN - 0102-9010
SP - 17-24
ST - Histochemical and ultrastructural analysis of the action of naproxen on tail
fin regeneration in carp (Cyprinus carpio)
T2 - Braz. j. morphol. sci
TI - Histochemical and ultrastructural analysis of the action of naproxen on tail
fin regeneration in carp (Cyprinus carpio)
UR - https://pesquisa.bvsalud.org/portal/resource/pt/lil-497613
VL - 24
Y2 - 3
ID - 8256
ER -
TY - JOUR
AB - Here, we review several articles of original research published in 2008 that
concerned the toxicity of metal and carbon-based nanoparticles. Articles were
selected from the MEDLINE PubMed database, all published or pre-published during
2008 and relating to nanomaterials, -particles or -structures and toxicity or
health. From the 746 articles, we concentrated on research into carbonaceous
(carbon nanotubes [CNTs] and fullerenes) and metallic materials (pure metal,
oxides), because these nanomaterials are produced and used worldwide and are the
most relevant for public health. Unfortunately, due to the large variability in
materials used and methods used conflicting data are generated hampering the risk
assessment. © 2010 Informa UK Ltd.
AN - rayyan-553780223
AU - Boczkowski, J.
AU - Hoet, P.
DO - 10.3109/17435390903428844
IS - 1
KW - Human health
Nanomaterials
Nanoparticles
Toxicity
Animals
Cell Line
Humans
Nanostructures
Nanotubes, Carbon
Public Health
PubMed
Review Literature as Topic
Risk Assessment
Toxicology
United States
carbon
carbon nanotube
catalase
CXCL2 chemokine
endothelial leukocyte adhesion molecule 1
fullerene derivative
gelatinase A
gelatinase B
glutathione
heme oxygenase 1
interleukin 1
interleukin 1beta
interleukin 6
interleukin 8
macrophage inflammatory protein 2
messenger RNA
metal nanoparticle
metal oxide
nanomaterial
silicon dioxide
silver
thioredoxin reductase
titanium dioxide
tumor necrosis factor alpha
unindexed drug
vascular cell adhesion molecule 1
cytotoxicity
genotoxicity
health hazard
human
inflammation
nanotoxicology
nonhuman
oxidative stress
priority journal
public health
review
risk assessment
toxicity testing
PY - 2010
SP - 1-14
ST - What's new in nanotoxicology? Implications for public health from a brief
review of the 2008 literature
T2 - Nanotoxicology
TI - What's new in nanotoxicology? Implications for public health from a brief
review of the 2008 literature
77749316839&doi=10.3109%2f17435390903428844&partnerID=40&md5=dfb002adcdaabbebcd50b0
708610a1e3
VL - 4
ID - 8257
ER -
TY - JOUR
AB - OBJECTIVE: Implantation of the mesh induces a foreign-body reaction followed
by the development of connective tissue that may alter tape property. The aim of
our study was to evaluate the deposition of collagen in the vicinity of
monofilament tension-free vaginal tape (TVT; Ethicon Inc., Johnson & Johnson) and
multifilament intravaginal slingplasty (IVS; Tyco Healthcare) polypropylene tapes
implanted in female rats. METHODS: The samples of the meshes (10 mg each) were
implanted in the rectus fascia of 14 Wistar female rats and removed after 42 days.
Collagen was extracted with 0.5 M acetic acid and subsequently with pepsin (1 mg/ml
in 0.5 M acetic acid). Collagen concentration was measured using Sircol Collagen
Assay (Biocolor Ltd.) and normalised for milligrams of tape weight. For
histological examination, tape samples were stained with haematoxylin and eosin or
with silver for type III collagen. RESULTS: The total amount of collagen extracted
did not differ significantly between TVT and IVS samples. For both tapes,
extraction with acetic acid yielded a higher amount of collagen (about 70%) than
extraction with pepsin. On histological examination, less densely packed bundles of
collagen fibres and a slightly more intense inflammatory reaction were observed
with TVT compared with IVS mesh. CONCLUSION: The total amount of collagen deposited
around the polypropylene mesh implanted in female rats was similar for TVT and IVS
meshes, but differences were noted in the arrangement of the collagen fibres and
the intensity of the inflammatory reaction.
AN - rayyan-553782206
AU - Bogusiewicz, M.
AU - Wróbel, A.
AU - Jankiewicz, K.
AU - Adamiak, A.
AU - Skorupski, P.
AU - Tomaszewski, J.
AU - Rechberger, T.
DO - 10.1016/j.ejogrb.2005.04.020
IS - 1
J2 - Eur J Obstet Gynecol Reprod Biol
KW - Animals
Biocompatible Materials/standards
Collagen/*biosynthesis
Connective Tissue/ultrastructure
Female
Foreign-Body Reaction/*etiology
Implants, Experimental/standards
Inflammation/physiopathology
Polypropylenes/standards
Rats
Rats, Wistar
Rectum/drug effects
Surgical Mesh/standards
Urinary Incontinence, Stress/pathology/surgery
Collagen
LA - eng
N1 - Second Department of Gynecology, University School of Medicine, Jaczewskiego
8, 20-954 Lublin, Poland. mbogusiewicz@yahoo.com
PY - 2006
SN - 0301-2115 (Print)
SP - 106-9
ST - Collagen deposition around polypropylene tapes implanted in the rectus fascia
of female rats
T2 - European journal of obstetrics, gynecology, and reproductive biology
TI - Collagen deposition around polypropylene tapes implanted in the rectus fascia
of female rats
VL - 124
Y2 - 1 y3 - 1
ID - 10116
ER -
TY - JOUR
AB - A rapid and convenient batch method for synthesizing lysine-conjugated silver
nanoparticles of approximately 5 nm of size was developed. Nanoparticles of size
less than 100 nm exhibit significant medical potential. L-Lysine demonstrates
potential for therapeutic applications and silver nanoparticles are an optimal
choice for drug delivery because of its intrinsic anti-platelet, anti-bacterial and
anti-inflammatory capabilities. Current synthesis protocols for Lysine-capped
particles under 10 nm are time consuming and tedious and allow only for the
sythesis of small quantities of particles. The synthesis of Lysin-capped silver
nanoparticles was based on the reaction in which AgNO(3) was reduced by excess
NaBH(4). L-Lysine, a known essential amino acid, served as the capping agent to
minimize initial aggregation. The particles were then separated by size
chromatography. Capping occurred through the amide bond on L-Lysine as determined
by FT-IR. The conjugation of the particle to the amide bond is important, since
this leaves the amino group of Lysine open to further modifications. The particles
were further characterized in regards to their shape, size and stability. Finally
we demonstrated that the synthesized particles exhibit limited to no toxicity in
cells, using HEK 293 cell line as a model system. Our sythesis protocol can be
successfully used for scale-up and synthesis of high quantities of nanoparticles.
AN - rayyan-553781873
AU - Bonor, J.
AU - Reddy, V.
AU - Akkiraju, H.
AU - Dhurjati, P.
AU - Nohe, A.
DO - 10.1166/asem.2014.1583
IS - 9
J2 - Adv Sci Eng Med
KW - Lysine
LA - eng
N1 - Department of Biological Sciences, University of Delaware, 321 Wolf Hall,
Newark, 19716, Delaware.; Department of Biological Sciences, University of
Delaware, 321 Wolf Hall, Newark, 19716, Delaware.; Department of Biological
Sciences, University of Delaware, 321 Wolf Hall, Newark, 19716, Delaware.;
Department of Chemical and Biomoleuclar Engineering, University of Delaware,
Newark, Delaware.; Department of Biological Sciences, University of Delaware, 321
Wolf Hall, Newark, 19716, Delaware.
PY - 2014
SN - 2164-6627 (Print)
SP - 942-947
ST - Synthesis and Characterization of L-Lysine Conjugated Silver Nanoparticles
Smaller Than 10 nM
T2 - Advanced science, engineering and medicine
TI - Synthesis and Characterization of L-Lysine Conjugated Silver Nanoparticles
Smaller Than 10 nM
VL - 6
Y2 - 9 y3 - 1
ID - 9797
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs), silver oxide nanoparticles (AgO-NPs), and zinc
oxide nanoparticles (ZnO-NPs) have healing, antibacterial, and antioxidant
properties. Furthermore, Ag-NPs and ZnO-NPs also have anti-inflammatory properties.
In this study, we synthesized a nanocomposite using Ag-ZnO and AgO-NPs (Ag-ZnO/AgO
NPs). The structural and morphological properties of nanocrystals and nanocomposite
were investigated by X-ray diffraction and scanning electronics microscopic. The
wurtzite crystalline structure of Ag-ZnO and two morphologies for the nanocomposite
(nanorods and nanoplatelets) were determined. Topical treatment with 1% Ag-ZnO/AgO
NPs was compared to untreated wounds (control group). Wounds were induced in the
dorsal region of BALB/c mice and evaluated after 3, 7, 14, and 21 days of
treatment. The nanocomposite demonstrated anti-inflammatory and antioxidant
capacities. In addition, wounds treated with Ag-ZnO/AgO NPs showed accelerated
closure, non-cytotoxicity, especially on keratinocytes and collagen deposition, and
increased metalloproteinases 2 and 9 activity. The nanocomposite improved healing
by reducing the inflammatory process, protecting tissues from damage caused by free
radicals, and increasing collagen deposition in the extracellular matrix. These
characteristics contributed to the accelerated wound closure process. Thus, Ag-
ZnO/AgO NPs show potential for can be a strategy for topical use in formulations of
new drugs to treat wounds.
AN - rayyan-553781785
AU - Borges Rosa de Moura, F.
AU - Antonio Ferreira, B.
AU - Helena Muniz, E.
AU - Benatti Justino, A.
AU - Gabriela Silva, A.
AU - de Azambuja Ribeiro, R. I. M.
AU - Oliveira Dantas, N.
AU - Lisboa Ribeiro, D.
AU - de Assis Araújo, F.
AU - Salmen Espindola, F.
AU - Christine Almeida Silva, A.
AU - Carla Tomiosso, T.
DO - 10.1016/j.ijpharm.2022.121620
J2 - Int J Pharm
KW - Animals
Anti-Bacterial Agents/chemistry/pharmacology
Anti-Inflammatory Agents/pharmacology
Antioxidants/pharmacology
*Metal Nanoparticles/chemistry
Mice
*Nanocomposites/chemistry
Oxides
Silver/pharmacology
Silver Compounds
Wound Healing
*Zinc Oxide/chemistry/pharmacology
Antioxidants
Zinc
LA - eng
N1 - Biomedical Sciences Institute, Federal University of Uberlândia, 38400-902,
Uberlândia-MG, Brazil; Biology Institute, State University of Campinas, 13083-862,
Campinas-SP, Brazil.; Biomedical Sciences Institute, Federal University of
Uberlândia, 38400-902, Uberlândia-MG, Brazil.; Biomedical Sciences Institute,
Federal University of Uberlândia, 38400-902, Uberlândia-MG, Brazil.; Biotechnology
Institute, Federal University of Uberlândia, 38405-319, Uberlândia-MG, Brazil.;
Laboratory of Experimental Pathology, Federal University of São João del-Rei,
35501-296, Divinópolis-MG, Brazil.; Laboratory of Experimental Pathology, Federal
University of São João del-Rei, 35501-296, Divinópolis-MG, Brazil.; Laboratory of
New Nanostructured and Functional Materials, Physics Institute, Federal University
of Alagoas, 57072-900, Maceió-AL, Brazil.; Biomedical Sciences Institute, Federal
University of Uberlândia, 38400-902, Uberlândia-MG, Brazil.; Biomedical Sciences
Institute, Federal University of Uberlândia, 38400-902, Uberlândia-MG, Brazil.;
Biotechnology Institute, Federal University of Uberlândia, 38405-319, Uberlândia-
MG, Brazil.; Laboratory of New Nanostructured and Functional Materials, Physics
Institute, Federal University of Alagoas, 57072-900, Maceió-AL, Brazil; Post-
Graduation Program in Northeast Network in Biotechnology, Federal University of
Alagoas, 57072-970 Maceió, AL, Brazil. Electronic address: acalmeida@fis.ufal.br.;
Biomedical Sciences Institute, Federal University of Uberlândia, 38400-902,
Uberlândia-MG, Brazil; Biology Institute, State University of Campinas, 13083-862,
Campinas-SP, Brazil. Electronic address: tatianatomiosso@ufu.br.
PY - 2022
SP - 121620
ST - Antioxidant, anti-inflammatory, and wound healing effects of topical silver-
doped zinc oxide and silver oxide nanocomposites
T2 - International journal of pharmaceutics
TI - Antioxidant, anti-inflammatory, and wound healing effects of topical silver-
doped zinc oxide and silver oxide nanocomposites
VL - 617
Y2 - 4 y3 - 5
ID - 9716
ER -
TY - JOUR
AB - The commensal microbiota plays a fundamental role in maintaining host gut
homeostasis by controlling several metabolic, neuronal and immune functions.
Conversely, changes in the gut microenvironment may alter the saprophytic microbial
community and function, hampering the positive relationship with the host. In this
bidirectional interplay between the gut microbiota and the host, hyaluronan (HA),
an unbranched glycosaminoglycan component of the extracellular matrix, has a
multifaceted role. HA is fundamental for bacterial metabolism and influences
bacterial adhesiveness to the mucosal layer and diffusion across the epithelial
barrier. In the host, HA may be produced and distributed in different cellular
components within the gut microenvironment, playing a role in the modulation of
immune and neuronal responses. This review covers the more recent studies
highlighting the relevance of HA as a putative modulator of the communication
between luminal bacteria and the host gut neuro-immune axis both in health and
disease conditions, such as inflammatory bowel disease and ischemia/reperfusion
injury. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780229
AU - Bosi, A.
AU - Banfi, D.
AU - Bistoletti, M.
AU - Moretto, P.
AU - Moro, E.
AU - Crema, F.
AU - Maggi, F.
AU - Karousou, E.
AU - Viola, M.
AU - Passi, A.
AU - Vigetti, D.
AU - Giaroni, C.
AU - Baj, A.
DO - 10.3390/cells11010126
IS - 1
KW - Enteric nervous system
Gastrointestinal tract
Gut microbiota
Hyaluronan
Immune system
Animals
Extracellular Matrix
Gastrointestinal Microbiome
Homeostasis
Humans
Hyaluronic Acid
Intestines
Neuroimmunomodulation
acetic acid
alpha defensin
beta defensin
butyric acid
chondroitin sulfate
claudin 2
collagen type 4
cyclooxygenase 2
dextran sulfate
entactin
fibronectin
fluorescein isothiocyanate
glial fibrillary acidic protein
glucose
glucuronic acid
glycosaminoglycan
heparin
Hermes antigen
hyaluronic acid
hyaluronic acid binding protein
hyaluronidase
immunoglobulin
immunoglobulin enhancer binding protein
inflammasome
interleukin 1
interleukin 17
interleukin 1beta
interleukin 22
interleukin 6
interleukin 8
long untranslated RNA
membrane protein
myeloid differentiation factor 88
myeloperoxidase
n acetylglucosamine
neuropeptide
neurotoxin
neurotransmitter
platelet derived growth factor receptor
polymer
polypeptide antibiotic agent
probiotic agent
propionic acid
protein ZO1
proteoglycan
reactive nitrogen species
silver nanoparticle
streptavidin
toll like receptor 2
transforming growth factor beta receptor 1
uridine diphosphate glucuronic acid
abdominal pain
Akkermansia muciniphila
amino acid sequence
angiogenesis
apoptosis
arthritis
B lymphocyte
bacterial metabolism
bacterial translocation
Bacteroides
basal lamina
Bifidobacterium
binding site
bleomycin-induced lung injury
blood vessel
carbon source
cartilage
cell adhesion
cell anchorage
cell division
cell membrane
cell migration
cell proliferation
cell surface
cell vacuole
central nervous system
chondrocyte
chromosome 11
Citrobacter rodentium
colon
commensal
Crohn disease
cytotoxic T lymphocyte
degenerative disease
dendritic cell
diarrhea
dietary fiber
drug metabolism
dysbiosis
elasticity
embryo development
endocytosis
Enterococcus faecium
epithelial mesenchymal transition
epithelium cell
Escherichia coli
eye surgery
Faecalibacterium
fibroblast
fibrosarcoma cell
gastrointestinal disease
gastrointestinal epithelium
gastrointestinal hemorrhage
gastrointestinal tract
gene expression
gene overexpression
hemolytic Streptococcus
homeostasis
humoral immunity
hydration
immune response
immune system
immunofluorescence
immunoreactivity
inflammatory bowel disease
intestine cell
intestine epithelium
intestine flora
intestine innervation
intestine lymphatic tissue
intestine mucosa
intestine transplantation
Lactobacillus
lamina propria
lymphocyte
macrophage
malnutrition
mast cell
microbial community
microenvironment
microvasculature
molecular weight
motor performance
multiple organ failure
natural killer cell
necrotizing enterocolitis
nerve cell
nerve cell plasticity
nervous system inflammation
neutrophil
NF kB signaling
nonhuman
osteoarthritis
oxidative stress
Paneth cell
Pasteurella multocida
phagocytosis
plasma cell
Prevotella oralis
radiosensitivity
reperfusion injury
respiratory failure
revascularization
Review
rhinopharyngitis
salmonellosis
sepsis
shock
small intestine
smooth muscle cell
Streptococcus pyogenes
superior mesenteric artery
systematic review
T lymphocyte
tissue injury
TLR signaling
tumor growth
ulcerative colitis
upregulation
vascular endothelium
vascular smooth muscle cell
wound healing
animal
human
immunomodulation
intestine
metabolism
pathology
PY - 2022
ST - Hyaluronan: A neuroimmune modulator in the microbiota-gut axis
T2 - Cells
TI - Hyaluronan: A neuroimmune modulator in the microbiota-gut axis
85122023921&doi=10.3390%2fcells11010126&partnerID=40&md5=484a75b15e53e12f66da994d62
04b0fa
VL - 11
ID - 8262
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) are highlighted due to their low toxicity,
compatibility with the human body, high surface area to volume ratio, and surfaces
that can be easily modified with ligands. Biosynthesis of AuNPs using plant extract
is considered a simple, low-cost, and eco-friendly approach. Brazilian Red Propolis
(BRP), a product of bees, exhibits anti-inflammatory, anti-tumor, antioxidant, and
antimicrobial activities. Here, we described the biosynthesis of AuNPs using BRP
extract (AuNPextract) and its fractions (AuNPhexane, AuNPdichloromethane, AuNPethyl
acetate) and evaluated their structural properties and their potential against
microorganisms and cancer cells. AuNPs showed a surface plasmon resonance (SPR)
band at 535 nm. The sizes and morphologies were influenced by the BRP sample used
in the reaction. FTIR and TGA revealed the involvement of bioactive compounds from
BRP extract or its fractions in the synthesis and stabilization of AuNPs.
AuNPdichloromethane and AuNPhexane exhibited antimicrobial activities against all
strains tested, showing their efficacy as antimicrobial agents to treat infectious
diseases. AuNPs showed dose-dependent cytotoxic activity both in T24 and PC-3
cells. AuNPdichloromethane and AuNPextract exhibited the highest in vitro cytotoxic
effect. Also, the cytotoxicity of biogenic nanoparticles was induced by mechanisms
associated with apoptosis. The results highlight a potential low-cost green method
using Brazilian red propolis to synthesize AuNPs, which demonstrated significant
biological properties.
AN - rayyan-553780230
AU - Botteon, C. E. A.
AU - Silva, L. B.
AU - Ccana-Ccapatinta, G. V.
AU - Silva, T. S.
AU - Ambrosio, S. R.
AU - Veneziani, R. C. S.
AU - Bastos, J. K.
AU - Marcato, P. D.
DO - 10.1038/s41598-021-81281-w
IS - 1
PY - 2021
SN - 2045-2322
ST - Biosynthesis and characterization of gold nanoparticles using Brazilian red
propolis and evaluation of its antimicrobial and anticancer activities
TI - Biosynthesis and characterization of gold nanoparticles using Brazilian red
propolis and evaluation of its antimicrobial and anticancer activities
VL - 11
Y2 - 1 y3 - 21
ID - 8263
ER -
TY - JOUR
AB - Environmentally induced perturbation of health parameters lead to
morphological changes associated to the inflammatory response. Hematoxyline and
eosin (H&E)-stained gill filaments sections were examined for such changes and
inflammation intensity was scored according to a quantitative model in order to
evaluate the health status of in vivo exposed (for 3, 6, and 12 h) mussels to
silver nanoparticles (Ag-NPs <50 nm and Ag-NPs <100 nm) prior and after the
inhibition of two potential uptake pathways (clathrin- and caveolae-mediated
endocytosis) with the aid of pharmaceutical inhibitors (amantadine and nystatin).
The impacts of the nanoparticles (NPs) size, as well as their uptake routes within
different time of exposure on the inflammatory response were assessed. The results
showed that Ag-NPs clearly induced morphological changes associated to the
inflammatory response in gill tissues (Mann-Whitney p values were <.05). It is also
clear that the length of the exposure as well as the NP size highly impacted
inflammation intensity (highest histopathological indices recorded with Ag-NPs
<100 nm). Also, the routes of NPs entry noticed to be major factor underlying
inflammatory response (significant inflammation intensity reported with Ag-NPs <50
nm after blockade of uptake routes; p <.05). Throughout, it was concluded that
inflammation intensity was related to NPs size and exposure time. Overall, uptake
routes are shown to be the major factor underlying nanotoxicity.
AN - rayyan-553782057
AU - Bouallegui, Y.
AU - Ben Younes, R.
AU - Bellamine, H.
AU - Oueslati, R.
DO - 10.1080/15376516.2017.1337258
IS - 8
J2 - Toxicol Mech Methods
KW - Animals
Bivalvia/*drug effects
Environmental Exposure
Gills/*drug effects/pathology
Inflammation/*chemically induced
Particle Size
Silver/*chemistry
Inflammation
LA - eng
N1 - a Research Unit of Immuno-Microbiology Environmental and Cancerogensis,
Sciences Faculty of Bizerte , University of Carthage, Carthage , Tunisia.; a
Research Unit of Immuno-Microbiology Environmental and Cancerogensis, Sciences
Faculty of Bizerte , University of Carthage, Carthage , Tunisia.; b Department of
Pathological Anatomy , Regional Hospital of Menzel Bourguiba , Bizerte , Tunisia.;
a Research Unit of Immuno-Microbiology Environmental and Cancerogensis, Sciences
Faculty of Bizerte , University of Carthage, Carthage , Tunisia.
PY - 2017
SP - 582-591
ST - Histopathology and analyses of inflammation intensity in the gills of mussels
exposed to silver nanoparticles: role of nanoparticle size, exposure time, and
uptake pathways
T2 - Toxicology mechanisms and methods
TI - Histopathology and analyses of inflammation intensity in the gills of mussels
exposed to silver nanoparticles: role of nanoparticle size, exposure time, and
uptake pathways
VL - 27
Y2 - 10
ID - 9971
ER -
TY - JOUR
AB - Purpose: Interstitial cystitis is a sterile bladder inflammatory disease
characterized by pelvic pain, urinary urgency and frequency. Nanocrystalline silver
has anti-inflammatory properties, prompting us to investigate its effect in
experimental bladder inflammation. Materials and Methods: Nanocrystalline silver
(0.01%, 0.05%, 0.1%, 0.5% or 1%) or phosphate buffered saline (Invitrogen (TM))
(0.5 ml) was introduced intravesically in Sprague-Dawley female rat (Charles River
Laboratories, Wilmington, Massachusetts) bladders for 20 minutes, followed by
vehicle or protamine sulfate (10 mg/ml for 30 minutes) and lipopolysaccharide
(Sigma (R)) (2 mg/ml for 45 minutes). Urine was collected. throughout for histamine
assay. The catheter was removed, the rat was returned to its cage and 4 hours later
it was sacrificed. The bladder was harvested, minced and cultured overnight. The
medium was collected for tumor necrosis factor-alpha assay. Results: Mean +/- SD
total urine histamine increased from 270 +/- 190 ng in 4 controls to 842 +/- 239 ng
after protamine sulfate/lipopolysaccharide and it decreased to 505 +/- 187 ng in 6
animals after pretreatment with 1% nanocrystalline silver (p = 0.036). Tumor
necrosis factor-a release in explant medium increased from 0.02 +/- 0.03 pg/mg in 6
controls to 0.28 +/- 0.15 pg/mg in 14 animals after treatment with protamine
sulfate/lipopolysaccharide and it decreased to 0.12 +/- 0.11 pg/mg in 10 animals
pretreated with nanocrystalline silver (p = 0.009). Nanocrystalline silver was not
effective at less than 1% and at 1% alone it released 0.05 +/- 0.07 pg/mg tumor
necrosis factor-alpha in 7 rats (vs phosphate buffered saline in 6, p = 0.387).
Nanocrystalline silver (1%) significantly decreased bladder inflammation and mast
cell activation. These effects were apparent even 4 days later. Conclusions:
Intravesical administration of nanocrystalline silver (1%) decreased urine
histamine, bladder tumor necrosis factor-a and mast cell activation without any
toxic effect. This action may be useful for interstitial cystitis.
AN - rayyan-553780231
AU - Boucher, W.
AU - Stern, J. M.
AU - Kotsinyan, V.
AU - Kempuraj, D.
AU - Papaliodis, D.
AU - Cohen, M. S.
AU - Theoharides, T. C.
DO - 10.1016/j.juro.2007.11.037
IS - 4
KW - Inflammation
PY - 2008
SN - 0022-5347 1527-3792
SP - 1598-1602
ST - Intravesical nanocrystalline silver decreases experimental bladder
inflammation
T2 - JOURNAL OF UROLOGY
TI - Intravesical nanocrystalline silver decreases experimental bladder
inflammation
VL - 179
Y2 - 4
ID - 8264
ER -
TY - JOUR
AB - Ingestion of engineered nanomaterials is inevitable due to their addition to
food and prevalence in food packaging and domestic products such as toothpaste and
sun cream. In the absence of robust dosimetry and particokinetic data, it is
currently challenging to accurately assess the potential toxicity of food-borne
nanomaterials. Herein, we review current understanding of gastrointestinal uptake
mechanisms, consider some data on the potential for toxicity of the most commonly
encountered classes of food-borne nanomaterials (including TiO2, SiO2 , ZnO, and Ag
nanoparticles), and discuss the potential impact of the luminal environment on
nanoparticle properties and toxicity. Much of our current understanding of
gastrointestinal nanotoxicology is derived from increasingly sophisticated
epithelial models that augment in vivo studies. In addition to considering the
direct effects of food-borne nanomaterials on gastrointestinal tissues, including
the potential role of chronic nanoparticle exposure in development of inflammatory
diseases, we also discuss the potential for food-borne nanomaterials to disturb the
normal balance of microbiota within the gastrointestinal tract. The latter
possibility warrants close attention given the increasing awareness of the critical
role of microbiota in human health and the known impact of some food-borne
nanomaterials on bacterial viability. WIREs Nanomed Nanobiotechnol 2018, 10:e1481.
doi: 10.1002/wnan.1481. This article is categorized under: Toxicology and
Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials. © 2017 The
Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals,
Inc.
AN - rayyan-553780232
AU - Bouwmeester, H.
AU - van der Zande, M.
AU - Jepson, M. A.
DO - 10.1002/wnan.1481
IS - 1
KW - Epithelium
Food
Gastrointestinal Tract
Humans
Kinetics
Microbiota
Nanostructures
Histology
Silica
Tissue
Titanium dioxide
Toxicity
Zinc oxide
silica nanoparticle
silver nanoparticle
nanomaterial
Bacterial viability
Gastrointestinal tract
Gastrointestinal uptakes
Inflammatory disease
Nanoparticle exposures
Nanoparticle properties
Potential impacts
dosimetry
food poisoning
gastrointestinal epithelium
gastrointestinal tissue
gastrointestinal tract
human
ingestion
intestine flora
kinetics
nonhuman
priority journal
Review
sedimentation
chemistry
epithelium
food
metabolism
microflora
physiology
Nanostructured materials
PY - 2018
ST - Effects of food-borne nanomaterials on gastrointestinal tissues and
microbiota
T2 - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
TI - Effects of food-borne nanomaterials on gastrointestinal tissues and
microbiota
85019711368&doi=10.1002%2fwnan.1481&partnerID=40&md5=021e5de7d85ef6ec819dc027cb8a3c
59
VL - 10
ID - 8265
ER -
TY - JOUR
AB - Bacterial cell wall endotoxins, i.e. lipopolysaccharides (LPS), are some of
the original compounds shown to evoke the classic signs of systemic
inflammation/innate immune response and neuroinflammation. The term
neuroinflammation often is used to infer the elaboration of proinflammatory
mediators by microglia elicited by neuronal targeted activity. However, it also is
possible that the microglia are responding to vasculature through several signaling
mechanisms. Microglial activation relative to the vasculature in the hippocampus
and parietal cortex was determined after an acute exposure of a single subcutaneous
injection of 2 mg/kg LPS. Antibodies to allograft inflammatory factor (Aif1, a.k.a.
Iba1) were used to track and quantify morphological changes in microglia.
Immunostaining of platelet/endothelial cell adhesion molecule 1 (Pecam1, a.k.a.
Cd31) was used to visualize vasculature in the forebrain and glial acidic
fibrillary protein (GFAP) to visualize astrocytes. Neuroinflammation and other
aspects of neurotoxicity were evaluated histologically at 3 h, 6 h, 12 h, 24 h, 3 d
and 14 d following LPS exposure. LPS did not cause neurodegeneration as determined
by Fluoro Jade C labeling. Also, there were no signs of mouse IgG leakage from
brain vasculature due to LPS. Some changes in microglia size occurred at 6 h, but
by 12 h microglial activation had begun with the combined soma and proximal
processes size increasing significantly (1.5-fold). At 24 h, almost all the
microglia soma and proximal processes in the hippocampus, parietal cortex, and
thalamus were closely associated with the vasculature and had increased almost 2.0-
fold in size. In many areas where microglia were juxtaposed to vasculature,
astrocytic endfeet appeared to be displaced. The microglial activation had subsided
slightly by 3 d with microglial size 1.6-fold that of control. We hypothesize that
acute LPS activation can result in vascular mediated microglial responses through
several mechanisms: 1) binding to Cd14 and Tlr4 receptors on microglia processes
residing on vasculature; 2) damaging vasculature and causing the release of
cytokines; and 3) possibly astrocytic endfeet damage resulting in cytokine release.
These acute responses may serve as an adaptive mechanism to exposure to circulating
LPS where the microglia surround the vasculature. This could further prevent the
pathogen(s) circulating in blood from entering the brain. However, diverting
microglial interactions away from synaptic remodeling and other types of microglial
interactions with neurons may have adverse effects on neuronal function.
AN - rayyan-553782335
AU - Bowyer, J. F.
AU - Sarkar, S.
AU - Burks, S. M.
AU - Hess, J. N.
AU - Tolani, S.
AU - O'Callaghan, J. P.
AU - Hanig, J. P.
DO - 10.1016/j.neuro.2020.01.014
J2 - Neurotoxicology
KW - Animals
Astrocytes/drug effects/immunology
Encephalitis/chemically induced/*immunology
Female
Hippocampus/*blood supply/drug effects/*immunology
Lipopolysaccharides/*toxicity
Mice, Inbred BALB C
Microglia/drug effects/*immunology
Prefrontal Cortex/*blood supply/drug effects/*immunology
LA - eng
N1 - Division of Neurotoxicology, National Center for Toxicology/ FDA, Jefferson,
AR 72079, USA.; Division of Neurotoxicology, National Center for Toxicology/ FDA,
Jefferson, AR 72079, USA. Electronic address: Sumit.Sarkar@fda.hhs.gov.; Division
of Neurotoxicology, National Center for Toxicology/ FDA, Jefferson, AR 72079, USA.;
Division of Neurotoxicology, National Center for Toxicology/ FDA, Jefferson, AR
72079, USA.; Division of Neurotoxicology, National Center for Toxicology/ FDA,
Jefferson, AR 72079, USA.; Health Effects Laboratory Division, Centers for Disease
Control and Prevention, National Institute for Occupational Safety and Health
Morgantown, WV 26505, USA.; Center for Drug Evaluation and Research/ FDA Silver
Spring, MD 20993, USA.
PY - 2020
SP - 181-192
ST - Microglial activation and responses to vasculature that result from an acute
LPS exposure
T2 - Neurotoxicology
TI - Microglial activation and responses to vasculature that result from an acute
LPS exposure
VL - 77
Y2 - 3
ID - 10243
ER -
TY - JOUR
AB - Background: Brain microglial activations and damage responses are most
commonly associated with neurodegeneration or systemic innate immune system
activation. Here, we used histological methods to focus on microglial responses
that are directed towards brain vasculature, previously undescribed, after a
neurotoxic exposure to methamphetamine. Methods: Male rats were given doses of
methamphetamine that produce pronounced hyperthermia, hypertension, and toxicity.
Identification of microglia and microglia-like cells (pericytes and possibly
perivascular cells) was done using immunoreactivity to allograft inflammatory
factor 1 (Aif1 a.k.a Iba1) and alpha M integrin (Itgam a.k.a. Cd11b) while
vasculature endothelium was identified using rat endothelial cell antigen 1 (RECA-
1). Regions of neuronal, axonal, and nerve terminal degeneration were determined
using Fluoro-Jade C. Results: Dual labeling of vasculature (RECA-1) and microglia
(Iba1) showed a strong association of hypertrophied cells surrounding and
juxtaposed to vasculature in the septum, medial dorsal hippocampus, piriform
cortex, and thalamus. The Iba1 labeling was more pronounced in the cell body while
Cd11b more so in the processes of activated microglia. These regions have been
previously identified to have vascular leakage after neurotoxic methamphetamine
exposure. Dual labeling with Fluoro-Jade C and Iba1 indicated that there was
minimal or no evidence of neuronal damage in the septum and hippocampus where many
hypertrophied Iba1-labeled cells were found to be associated with vasculature.
Although microglial activation around the prominent neurodegeneration was found in
the thalamus, there were also many examples of activated microglia associated with
vasculature. Conclusions: The data implicate microglia, and possibly related cell
types, in playing a major role in responding to methamphetamine-induced vascular
damage, and possibly repair, in the absence of neurodegeneration. Identifying brain
regions with hypertrophied/activated microglial-like cells associated with
vasculature has the potential for identifying regions of more subtle examples of
vascular damage and BBB compromise.
AN - rayyan-553780233
AU - Bowyer, J. F.
AU - Sarkar, S.
AU - Tranter, K. M.
AU - Hanig, J. P.
AU - Miller, D. B.
AU - O'Callaghan, J. P.
DO - 10.1186/s12974-016-0526-6
KW - Microglia
Methamphetamine
PY - 2016
SN - 1742-2094
ST - Vascular-directed responses of microglia produced by methamphetamine
exposure: indirect evidence that microglia are involved in vascular repair?
T2 - JOURNAL OF NEUROINFLAMMATION
TI - Vascular-directed responses of microglia produced by methamphetamine
exposure: indirect evidence that microglia are involved in vascular repair?
VL - 13
Y2 - 3 y3 - 12
ID - 8266
ER -
TY - JOUR
AB - A number of studies have shown that induction of pulmonary toxicity by
nanoparticles of the same chemical composition depends on particle size, which is
likely in part due to differences in lung deposition. Particle size mostly
determines whether nanoparticles reach the alveoli, and where they might induce
toxicity. For the risk assessment of nanomaterials, there is need for a suitable
dose metric that accounts for differences in effects between different sized
nanoparticles of the same chemical composition. The aim of the present study is to
determine the most suitable dose metric to describe the effects of silver
nanoparticles after short-term inhalation. Rats were exposed to different
concentrations (ranging from 41 to 1105 μg silver/m3 air) of 18, 34, 60 and 160 nm
silver particles for four consecutive days and sacrificed at 24 h and 7 days after
exposure. We observed a concentration-dependent increase in pulmonary toxicity
parameters like cell counts and pro-inflammatory cytokines in the bronchoalveolar
lavage fluid. All results were analysed using the measured exposure concentrations
in air, the measured internal dose in the lung and the estimated alveolar dose. In
addition, we analysed the results based on mass, particle number and particle
surface area. Our study indicates that using the particle surface area as a dose
metric in the alveoli, the dose-response effects of the different silver particle
sizes overlap for most pulmonary toxicity parameters. We conclude that the alveolar
dose expressed as particle surface area is the most suitable dose metric to
describe the toxicity of silver nanoparticles after inhalation. © 2015 Informa UK
Ltd.
AN - rayyan-553780234
AU - Braakhuis, H. M.
AU - Cassee, F. R.
AU - Fokkens, P. H. B.
AU - De La Fonteyne, L. J. J.
AU - Oomen, A. G.
AU - Krystek, P.
AU - De Jong, W. H.
AU - Van Loveren, H.
AU - Park, M. V. D. Z.
DO - 10.3109/17435390.2015.1012184
IS - 1
KW - Dose metrics
inhalation exposure
nanotoxicology
risk assessment
Animals
Bronchoalveolar Lavage Fluid
Cytokines
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Oxidative Stress
Particle Size
Pneumonia
Rats
Rats, Inbred F344
Silver
granulocyte macrophage colony stimulating factor
interleukin 12p70
interleukin 1beta
RANTES
silver nanoparticle
cytokine
metal nanoparticle
silver
animal experiment
animal model
Article
cell count
controlled study
dose response
dosimetry
humidity
inhalational drug administration
leukocyte differential count
lung burden
lung clearance
lung toxicity
male
nonhuman
oxidative stress
particle size
pneumonia
premature mortality
priority journal
rat
surface area
animal
chemically induced
cytology
drug effects
exposure
Fischer 344 rat
immunology
lung
metabolism
Inflammation
Metronidazole
PY - 2016
SP - 63-73
ST - Identification of the appropriate dose metric for pulmonary inflammation of
silver nanoparticles in an inhalation toxicity study
T2 - Nanotoxicology
TI - Identification of the appropriate dose metric for pulmonary inflammation of
silver nanoparticles in an inhalation toxicity study
84959553835&doi=10.3109%2f17435390.2015.1012184&partnerID=40&md5=7193c7b4fcb52ec837
e2ef3a2115dbb1
VL - 10
ID - 8267
ER -
TY - JOUR
AB - Consumers are orally exposed to nanoparticulate or soluble species of the
non-essential element silver due to its use in food contact materials or as a food
additive. Potential toxicity of silver nanoparticles has gained special scientific
attention. A fraction of ingested ionic or particulate silver is taken up in the
intestine and transported to the liver, where it may induce oxidative stress and
elicit subsequent adverse responses. Here, we present a comprehensive analysis of
global proteomic changes induced in human Hep G2 hepatocarcinoma cells by different
concentrations of AgPURE silver nanoparticles or by corresponding concentrations of
ionic silver. Bioinformatic analysis of proteomic data confirms and substantiates
previous findings on silver-induced alterations related to redox stress,
mitochondrial dysfunction, intermediary metabolism, inflammatory responses,
posttranslational protein modification and other cellular parameters. Similarities
between the effects exerted by the two silver species are in line with the
assumption that silver ions released from nanoparticles substantially contribute to
their toxicity. Moreover, a comparative bioinformatic evaluation of proteomic
effects in hepatic and intestinal cells exerted either by silver nanoparticles or
bionic silver is presented. Our results show that, despite remarkable differences
at the level of affected proteins in the different cell lines, highly similar
biological consequences, corresponding to previous in vivo findings, can be deduced
by applying appropriate bioinformatic data mining. Copyright © 2017 John Wiley &
Sons, Ltd.
AN - rayyan-553780237
AU - Braeuning, A.
AU - Oberemm, A.
AU - Görte, J.
AU - Böhmert, L.
AU - Juling, S.
AU - Lampen, A.
DO - 10.1002/jat.3568
IS - 5
KW - Caco-2
Hep G2
molecular toxicity
nanotoxicology
proteomics
Electrophoresis, Gel, Two-Dimensional
Hep G2 Cells
Humans
Intestines
Liver
Mass Spectrometry
Metal Nanoparticles
Proteomics
Silver Compounds
silver nanoparticle
silver nitrate
metal nanoparticle
silver derivative
Article
bioinformatics
Caco-2 cell line
comparative study
cytotoxicity
Hep-G2 cell line
human
human cell
inflammation
intestine cell
liver cell
metabolism
oxidative stress
priority journal
protein processing
redox stress
cytology
drug effect
intestine
liver
mass spectrometry
two dimensional gel electrophoresis
Humanities
Humanism
Proteome
PY - 2018
SP - 638-648
ST - Comparative proteomic analysis of silver nanoparticle effects in human liver
and intestinal cells
TI - Comparative proteomic analysis of silver nanoparticle effects in human liver
85044444604&doi=10.1002%2fjat.3568&partnerID=40&md5=b56193292b3ddc318e18fb279482360
6
VL - 38
ID - 8270
ER -
TY - JOUR
AB - Consumers are orally exposed to nanoparticulate or soluble species of the
non-essential element silver due to its use in food contact materials or as a food
additive. Potential toxicity of silver nanoparticles has gained special scientific
attention. A fraction of ingested ionic or particulate silver is taken up in the
intestine and transported to the liver, where it may induce oxidative stress and
elicit subsequent adverse responses. Here, we present a comprehensive analysis of
global proteomic changes induced in human Hep G2 hepatocarcinoma cells by different
concentrations of AgPURE silver nanoparticles or by corresponding concentrations of
ionic silver. Bioinformatic analysis of proteomic data confirms and substantiates
previous findings on silver-induced alterations related to redox stress,
mitochondrial dysfunction, intermediary metabolism, inflammatory responses,
posttranslational protein modification and other cellular parameters. Similarities
between the effects exerted by the two silver species are in line with the
assumption that silver ions released from nanoparticles substantially contribute to
their toxicity. Moreover, a comparative bioinformatic evaluation of proteomic
effects in hepatic and intestinal cells exerted either by silver nanoparticles or
bionic silver is presented. Our results show that, despite remarkable differences
at the level of affected proteins in the different cell lines, highly similar
biological consequences, corresponding to previous in vivo findings, can be deduced
by applying appropriate bioinformatic data mining. Silver nanoparticles and ionic
silver exert toxicity in cells. Consumers are orally exposed to silver from food
packaging or food additives. Here, a proteomic analysis of effects of AgPURE silver
nanoparticles and ionic silver in Hep G2 hepatocarcinoma cells is presented. Data
confirm the important role of oxidative stress in silver toxicity. A comparative
analysis of silver effects in hepatic and intestinal cells shows how bioinformatic
can help to deduce similar biological effects from remarkably differing data sets.
AN - rayyan-553780238
AU - Braeuning, A.
AU - Oberemm, A.
AU - Gorte, J.
AU - Bohmert, L.
AU - Juling, S.
AU - Lampen, A.
DO - 10.1002/jat.3568
IS - 5
KW - Humanities
Humanism
Humans
Proteomics
Proteome
PY - 2018
SN - 0260-437X 1099-1263
SP - 638-648
ST - Comparative proteomic analysis of silver nanoparticle effects in human liver
T2 - JOURNAL OF APPLIED TOXICOLOGY
TI - Comparative proteomic analysis of silver nanoparticle effects in human liver
VL - 38
Y2 - 5
ID - 8271
ER -
TY - JOUR
AB - Oral lichen planus (OLP) is a chronic inflammatory disease with different
clinical types. Reticular and erosive forms are the most common. Although the cause
of OLP remains speculative, many findings suggest auto-immune involvement, mediated
by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma
or toxic agents can affect the epithelial homeostasia. Increased apoptosis may
cause a decrease in epithelial thickness reflecting in the activity of the lesion.
The objective of this study was to evaluate the occurrence of apoptosis and
epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each
type (reticular and erosive) plus 10 of healthy mucosa were collected and
processed. After morphometry, the apoptotic index and epitelial thickness were
obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate
the morphologic criteria used. Apoptosis in the erosive OLP was significantly more
intense than in the reticular type and both forms of OLP presented more apoptosis
than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms
and thicker in OLP reticular form than in the erosive one. The clinical differences
between reticular and erosive forms of OLP are related to variations in epithelial
thickness and in intensity of apoptosis. O líquen plano oral (LPO) é uma doença
inflamatória crônica com diferentes tipos clínicos. As mais comuns são as formas
reticular e erosiva. Embora a causa do LPO permaneça no campo especulativo, muitos
achados sugerem tratar-se de uma doença auto-imune, mediada por linfócitos T que
têm como alvo os ceratinócitos basais. Inflamação, trauma mecânico ou agentes
tóxicos podem afetar a homeostasia epitelial. O aumento da apoptose pode levar a
uma diminuição da espessura epitelial e isto refletir na atividade da doença. O
objetivo deste estudo foi avaliar a ocorrência de apoptose e a espessura epitelial
nas formas reticular e erosiva de LPO. 15 amostras de LPO de cada tipo reticular e
erosivo, além de 10 amostras de mucosa saudável foram coletadas e processadas.
Depois da morfometria, o índice apoptótico (IA) e a espessura do epitélio foram
obtidas. Reação de TUNEL e imunohistoquímica do M30 CytoDeath foram usadas para
validação dos critérios morfológicos. A apoptose no LPO erosivo foi
significativamente maior que no tipo reticular e ambas as formas de LPO
apresentaram mais apoptose que a mucosa oral normal. A mucosa oral normal foi mais
espessa que ambas as formas de LPO, sendo que, a forma reticular foi mais espessa
que o tipo erosivo. As diferenças clínicas entre as formas reticular e erosiva de
LPO têm relação com as variações na espessura epitelial e na intensidade da
apoptose.
AN - rayyan-553780239
AU - Brant, Juliana M. Caldeira
AU - Vasconcelos, Anilton C.
AU - Rodrigues, Luciana V.
IS - 3
KW - Apoptosis
M30 CytoDEATH
Oral lichen planus
TUNEL
Lichen Planus
LA - en
PY - 2008
SN - 0103-6440
SP - 179-185
ST - Role of apoptosis in erosive and reticular oral lichen planus exhibiting
variable epithelial thickness
T2 - Braz. dent. j
TI - Role of apoptosis in erosive and reticular oral lichen planus exhibiting
variable epithelial thickness
64402008000300001
VL - 19
ID - 8272
ER -
TY - JOUR
AB - The incorporation of engineered nanoparticles (NPs) into everyday consumer
goods, products, and applications has given rise to the field of nanotoxicology,
which evaluates the safety of NPs within biological environments. The unique
physicochemical properties of NPs have made this an insurmountable challenge, as
their reactivity and variable behavior have given rise to discrepancies between
standard cell-based in vitro and animal in vivo models. In this study, enhanced in
vitro models were generated that retained the advantages of traditional cell
cultures, but incorporated the modifications of (1) inclusion of an activated
immune element and (2) the presence of physiologically-relevant dynamic flow.
Following verification that the human alveolar epithelial and macrophage
(A549/U937) co-culture could be successfully sustained under both static and
dynamic conditions, these cultures, in addition to a standard A549 static model,
were challenged with 10 nm citrate coated silver NPs (AgNPs). This work identified
a reshaping of the AgNP-cellular interface and differential biological responses
following exposure. The presence of dynamic flow modified cellular morphology and
reduced AgNP deposition by approximately 20% over the static exposure environments.
Cellular toxicity and stress endpoints, including reactive oxygen species, heat
shock protein 70, and secretion of pro-inflammatory cytokines, were found to vary
as a function of both cellular composition and flow conditions; with activated
macrophages and fluid flow both mitigating the severity of AgNP-dependent
bioeffects. This work highlights the possibility of enhanced in vitro systems to
assess the safety of engineered NPs and demonstrates their effectiveness in
elucidating novel NP-cellular interactions and toxicological profiles. © 2021 by
the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780241
AU - Braun, N. J.
AU - Galaska, R. M.
AU - Jewett, M. E.
AU - Krupa, K. A.
DO - 10.3390/nano11071807
IS - 7
KW - Cellular co-culture
Cytokine production
Dynamic flow
Nanotoxicology
Silver nanoparticle
PY - 2021
ST - Implementation of a dynamic co-culture model abated silver nanoparticle
interactions and nanotoxicological outcomes in vitro
T2 - Nanomaterials
TI - Implementation of a dynamic co-culture model abated silver nanoparticle
interactions and nanotoxicological outcomes in vitro
85109542954&doi=10.3390%2fnano11071807&partnerID=40&md5=6d1e55eaa20d9f766c2b02e38a7
1194e
VL - 11
ID - 8274
ER -
TY - JOUR
AB - Since current purification methods cannot completely remove all traces of
endotoxin in biomaterials intended for use in implantable or blood-contacting
devices, acceptable levels of endotoxin contamination that will not cause a
significant inflammatory reaction need to be defined. Inflammatory reactions to
biomaterials may include production of high concentrations of potentially harmful
nitric oxide (NO) generated by macrophages. Nitrite accumulation was measured from
RAW264.7 cells treated with either lipopolysaccharide (LPS) free in solution or
defined quantities of LPS incorporated into alginate in the absence or presence of
murine interferon-gamma (mrIFN-gamma). In the absence of IFN-gamma, significant NO
production by RAW 264.7 cells occurred for LPS levels down to 0.018 EU/mL. In the
presence of mrIFN-gamma, the lowest concentration of LPS tested in solution (0.006
EU/mL) elicited a significant increase in NO production. In the absence or presence
of mrIFN-gamma, five times the concentration of LPS incorporated into alginate as
compared to LPS free in solution was necessary to elicit a similar NO response by
RAW264.7. These results demonstrate that very low concentrations of endotoxin can
elicit significant NO responses from macrophages, particularly when inflammatory
cytokines are present. Biomaterials may sequester endotoxin, resulting in lower
inflammatory reactions that otherwise might be expected.
AN - rayyan-553782277
AU - Breger, J. C.
AU - Lyle, D. B.
AU - Shallcross, J. C.
AU - Langone, J. J.
AU - Wang, N. S.
DO - 10.1002/jbm.b.31452
IS - 2
J2 - J Biomed Mater Res B Appl Biomater
KW - Alginates/*chemistry
Animals
Capsules
*Drug Contamination
Endotoxins/*toxicity
Inflammation/*chemically induced/*pathology
Interferon-gamma/pharmacology
Macrophages/drug effects/metabolism
Mice
Nitric Oxide/metabolism
No-Observed-Adverse-Effect Level
Risk Assessment
Alginates
LA - eng
N1 - Department of Chemical and Biomolecular Engineering, University of Maryland,
College Park, Maryland 20742.; Center for Devices and Radiological Health, Office
of Science and Engineering Laboratories, FDA, Silver Spring, Maryland 20993-002.;
Center for Devices and Radiological Health, Office of Science and Engineering
Laboratories, FDA, Silver Spring, Maryland 20993-002.; Center for Devices and
Radiological Health, Office of Science and Engineering Laboratories, FDA, Silver
Spring, Maryland 20993-002.; Center for Devices and Radiological Health, Office of
Science and Engineering Laboratories, FDA, Silver Spring, Maryland 20993-002.;
Department of Chemical and Biomolecular Engineering, University of Maryland,
College Park, Maryland 20742.
PY - 2009
SP - 755-765
ST - Defining critical inflammatory parameters for endotoxin impurity in
manufactured alginate microcapsules
T2 - Journal of biomedical materials research. Part B, Applied biomaterials
TI - Defining critical inflammatory parameters for endotoxin impurity in
manufactured alginate microcapsules
VL - 91
Y2 - 11
ID - 10187
ER -
TY - JOUR
AB - ABSTRACT Aims and Objectives: Polypropylene meshes have been increasingly
adopted for correction of pelvic organ prolapse due to its lower recurrence rate
when compared to surgeries without meshes. The study of the interaction of these
materials with the host tissue may contribute to the development of materials with
best biocompatibility and, consequently, less complication rates. Materials and
Methods: The present study compares the inflammatory reaction of standard-weight
(SW) and lightweight (LW) meshes (72 g/m216g/m2 respectively), implanted in the
abdomen of 20 adult rats, which were euthanized in four or 30 days. Quantification
of pro-inflammatory markers, IL-1 and TNF-α, and of metalloproteinases, MMP2 and
MMP3, were carried out through immunohistochemistry with AxioVision® software.
Results: There were no significant differences in the quantification of IL-1 and
TNF-α in LW versus SW meshes. However, IL-1 quantification increased along time (30
days >4 days, p=0.0269). Also, MMP-2 quantification was similar to SW and LW and
both presented a significant increase along time (30 days >4 days, p <0.0001). MMP-
3 quantification also showed no difference between the SW and LW groups, but
increased along time (30 days >4 days, p=0.02). Conclusions: Mesh's density did not
influence the quantification of pro-inflammatory cytokines IL-1 and TNF-α and
metalloproteinases 2 and 3. The increased expression of IL-1, MMP-2 and MMP-3 over
time could represent a longstanding inflammatory response after PP mesh
implantation. Possibly, the occurrence of adverse events following PP prosthetic
implants can be influenced by other factors, not solely related to the amount of
implanted material.
AN - rayyan-553780243
AU - Bronzatto, Elaine
AU - Riccetto, Cássio Luis Zanettini
DO - 10.1590/s1677-5538.ibju.2016.0553
IS - 4
KW - Cytokines
Metalloproteases
Polyamide mesh [Supplementary Concept]
Polypropylenes
Rats
LA - en
PY - 2018
SN - 1677-5538
SP - 819-825
ST - Pro - inflammatory cytokines and metalloproteinase activation in
polypropylene mesh implant in rat subcutaneous tissue
T2 - Int. braz. j. urol
TI - Pro - inflammatory cytokines and metalloproteinase activation in
polypropylene mesh implant in rat subcutaneous tissue
55382018000400819
VL - 44
Y2 - 8 y3 - 1
ID - 8276
ER -
TY - JOUR
AB - Stenotrophomonas maltophilia is an opportunistic pathogen of significant
concern to susceptible patient populations. This pathogen can cause nosoco-mial and
community-acquired respiratory and bloodstream infections and various other
infections in humans. Sources include water, plant rhizospheres, animals, and
foods. Studies of the genetic heterogeneity of S. maltophilia strains have
identified several new genogroups and suggested adaptation of this pathogen to its
habitats. The mechanisms used by S. maltophilia during pathogenesis continue to be
uncov-ered and explored. S. maltophilia virulence factors include use of motility,
biofilm forma-tion, iron acquisition mechanisms, outer membrane components, protein
secretion sys-tems, extracellular enzymes, and antimicrobial resistance mechanisms.
S. maltophilia is intrinsically drug resistant to an array of different antibiotics
and uses a broad arsenal to protect itself against antimicrobials. Surveillance
studies have recorded increases in drug resistance for S. maltophilia, prompting
new strategies to be developed against this op-portunist. The interactions of this
environmental bacterium with other microorganisms are being elucidated. S.
maltophilia and its products have applications in biotechnology, including
agriculture, biocontrol, and bioremediation. © 2021 American Society for
Microbiology. All Rights Reserved.
AN - rayyan-553780244
AU - Brooke, J. S.
DO - 10.1128/CMR.00030-19
IS - 3
KW - Antimicrobial agents
Antimicrobial resistance
Biofilms
Biotechnology
Cystic fibrosis
Genomes
Pathogenesis
Risk factors
S. maltophilia
Stenotrophomonas
Animals
Gram-Negative Bacterial Infections
Humans
Stenotrophomonas maltophilia
Virulence Factors
abamectin
acaricide
acetylcysteine
amikacin
aminoglycoside
antibiotic agent
avibactam
azithromycin
bacteriocin
beta lactamase AmpC
beta lactamase inhibitor
bilirubin
bilirubin glucuronide
caffeic acid
carbapenem
carbapenemase
carbendazim
catalase
cefepime
cefotaxime
ceftazidime
chloramphenicol
cinnamic acid
ciprofloxacin
colistin
cotrimoxazole
cyclopeptide
deflazacort
echinocandin
elongation factor G
erythromycin
ferulic acid
flagellin
FtsZ protein
gamma interferon
gentamicin
glycosyltransferase
hyaluronic acid
hyaluronidase
imipenem
kanamycin
lactoferrin
levofloxacin
macrolide
meropenem
minocycline
moxifloxacin
mycotoxin
nanocomposite
outer membrane protein
paraquat
peptidomimetic agent
piperacillin plus tazobactam
polymyxin B
quinoline derived antiinfective agent
quinolone derivative
rifampicin
RNA 16S
ropocamptide
signal peptidase
silver nanoparticle
spiramycin
tetracycline
ticarcillin
tobramycin
triclosan
virulence factor
antiinfective agent
Actinia equina
agriculture
Anemonia sulcata
antibiotic resistance
bacteremia
bacterial virulence
biofilm
bioinformatics
biological pest control
bioremediation
bloodstream infection
bone disease
coinfection
controlled study
cystic fibrosis
cytotoxicity
DNA microarray
endocarditis
endophthalmitis
enterocolitis
eye infection
febrile neutropenia
fluorescence in situ hybridization
gas chromatography
gene sequence
genetic heterogeneity
genotype
hematologic malignancy
high throughput analysis
hospital infection
human
hypoalbuminemia
immunofluorescence assay
liquid chromatography-mass spectrometry
matrix assisted laser desorption ionization time of flight mass spectrometry
meningitis
methicillin resistant Staphylococcus aureus
microbiology
multidrug resistance
multiplex polymerase chain reaction
multiplex real time polymerase chain reaction
neurologic disease
neutropenic enterocolitis
nonhuman
osteomyelitis
outer membrane
protein secretion
quantitative methylation specific polymerase chain reaction
quorum sensing
respiratory failure
respiratory system
Review
rhizosphere
risk factor
RNA sequencing
septic shock
soft tissue disease
spinal cord disease
ventilator associated pneumonia
whole genome sequencing
animal
genetics
Gram negative infection
PY - 2021
ST - Advances in the microbiology of stenotrophomonas maltophilia
T2 - Clinical Microbiology Reviews
TI - Advances in the microbiology of stenotrophomonas maltophilia
85108386768&doi=10.1128%2fCMR.00030-
19&partnerID=40&md5=9129e11c4a7991498a1527e087e28d16
VL - 34
ID - 8277
ER -
TY - JOUR
AB - A major user of nanoparticles (NPs) is the pigment and ink industry, where
NPs are incorporated into numerous products (e.g. paints, food, plastics, printers,
personal care products, and construction materials). Assessment of NP toxicity
requires potential impacts on human health and the environment to be evaluated. In
this study, we examined the toxicity of a range of NPs, of varied physico-chemical
properties, used in the pigment and ink industries including silver (Ag), iron
oxide (Fe2O3), titanium dioxide (TiO2), aluminium oxide (Al2O3), zinc oxide (ZnO),
cobalt aluminium oxide (CoAl2O4) and cadmium selenide/zinc sulphide (CdSe/ZnS)
quantum dots (QDs). Acute toxicity exerted by this NP panel to mammalian cells in
vitro (macrophages, hepatocytes and alveolar epithelial cells) and aquatic
environmental organisms (Raphidocelis subcapitata Daphnia magna, Lumbriculus
variegatus) was investigated. For mammalian cells, cytotoxicity was assessed 24 h
post exposure, at concentrations ranging from 1 to 125 μg/ml using the LDH and WST-
1 assays. The aquatic toxicity of the NP panel was assessed according to OECD
protocols (201, 202, 315), up to 96 h post exposure. Rats were exposed to selected
NPs via intratracheal instillation (62 μg) and the pulmonary inflammatory response
quantified 24 h post exposure. This cross-species comparison revealed that Ag, QDs
and ZnO NPs were consistently more toxic than the other NPs tested. By looking
across mammalian and aquatic ecotoxicological models we obtained a better
understanding of the sensitivity of each model, and thus which models should be
prioritised for selection in the future when assessing the mammalian and
ecotoxicity of NPs, and in particular when screening the toxicity of a panel of
NPs. We recommend that macrophage and daphnia models are prioritised when assessing
the mammalian toxicity and ecotoxicity of NPs, respectively, due to their increased
sensitivity, compared to the other models tested. Of interest is that the in vitro
and invertebrate models used were able to predict the toxic potency of the NPs in
rodents, and thus our approach has the potential to enhance the implementation of
the 3Rs principles in nanotoxicology and reduce reliance on rodent testing when
assessing NP safety. By identifying hazardous NPs the data obtained from this study
can feed into the selection of (low toxicity) NPs to use in products and will also
contribute to the safe design of future generations of NPs used by the pigment and
ink industries. © 2018
AN - rayyan-553780245
AU - Brown, D. M.
AU - Johnston, H. J.
AU - Gaiser, B.
AU - Pinna, N.
AU - Caputo, G.
AU - Culha, M.
AU - Kelestemur, S.
AU - Altunbek, M.
AU - Stone, V.
AU - Roy, J. C.
AU - Kinross, J. H.
AU - Fernandes, T. F.
DO - 10.1016/j.impact.2018.02.001
KW - Cross-species models
In vitro
Nanoparticles
Toxicity testing
Aluminum
Aluminum compounds
Aquatic organisms
Cadmium compounds
Cell culture
Chemical industry
Cobalt compounds
Iron oxides
Macrophages
Mammals
Oxides
Plastics industry
Product design
Safety testing
Selenium compounds
Semiconductor quantum dots
Silver
Silver compounds
Sulfur compounds
Titanium dioxide
Titanium oxides
Zinc compounds
Zinc oxide
Zinc sulfide
Alveolar epithelial cells
Cross-species
Cross-species comparisons
In-vitro
Physicochemical property
Titanium dioxides (TiO2)
Toxicity
PY - 2018
SP - 20-32
ST - A cross-species and model comparison of the acute toxicity of nanoparticles
used in the pigment and ink industries
T2 - NanoImpact
TI - A cross-species and model comparison of the acute toxicity of nanoparticles
used in the pigment and ink industries
85041380349&doi=10.1016%2fj.impact.2018.02.001&partnerID=40&md5=b1f42f4e720461d16e4
d6cdda4a05820
VL - 11
ID - 8278
ER -
TY - JOUR
AB - Major molecular mechanisms that underpin the toxicity of nanoparticles (NPs)
are the formation of reactive oxygen species and the induction of inflammation. The
latter is frequently observed in vitro and in mammalian organisms, yet in aquatic
organisms, such NP-induced inflammatory responses remain largely unexplored.
Zebrafish offer a wide range of molecular tools to investigate immune responses in
an aquatic organism, and were therefore used here to describe how copper (Cu) NPs
(25 nm; 1 mg L-1) and soluble Cu as well as polystyrene (PS) NPs (25 nm; 10 mg L1-)
induce innate immune responses, focussing on the skin cells and the intestine as
likely organs of interaction. mRNA expression of the immune responsive genes
interleukin 1 beta (il1 beta) and immunoresponsive gene 1-like (irg1l) of CuNP
exposed embryos was observed to be weaker in the intestinal tissue compared to the
rest of the body, indicating a strong outer epithelium response. Specifically, NPs
were observed to accumulate in the cavities of lateral neuromasts in the skin,
which coincided with an increased local expression of il1 beta. Exposure to CuNPs
triggered the strongest transcriptional changes in pro-inflammatory-related genes
and was also observed to increase migration of neutrophils in the tail, indicating
a NP-specific inflammatory response. This is the first in vivo evidence for
waterborne NP exposure triggering alterations of immune system regulating genes in
the skin and intestine of zebrafish embryos. The observed molecular responses have
the potential to be linked to adverse effects at higher levels of biological
organization and hence might be used for screening purposes in nanotoxicology or as
building blocks for adverse outcome pathways.
AN - rayyan-553780246
AU - Brun, N. R.
AU - Koch, B. E. V.
AU - Varela, M.
AU - Peijnenburg, Wjgm
AU - Spaink, H. P.
AU - Vijver, M. G.
DO - 10.1039/c8en00002f
IS - 4
KW - Immune System
PY - 2018
SN - 2051-8153 2051-8161
SP - 904-916
ST - Nanoparticles induce dermal and intestinal innate immune system responses in
zebrafish embryos
T2 - ENVIRONMENTAL SCIENCE-NANO
TI - Nanoparticles induce dermal and intestinal innate immune system responses in
zebrafish embryos
VL - 5
Y2 - 4 y3 - 1
ID - 8279
ER -
TY - CHAP
AB - Glioblastoma multiforme (GBM) is one of the most aggressive types of brain
tumor in humans. The prognosis for patients with GBM is unfavorable and treatment
is largely ineffective, where modern treatment regimens typically increase survival
by 15 months. GBM relapse and progression are associated with cancer stem cells
(CSCs). The present review provides a critical analysis of the primary reasons
underlying the lack of effectiveness of modern CSC management methods. An emphasis
is placed on the role of the blood-brain barrier in the development of treatment
resistance. The existing methods for increasing the efficiency of antitumor
genotoxic therapy are also described, and a strategy for personalized regulation of
CSC based on post-genome technologies is suggested. The hypothesis that GBM cells
employ a special mechanism for DNA repair based on their interactions with normal
stem cells, is presented and the function of the tumor microenvironment in
fulfilling the antitumor potential of normal stem cells is explained. Additionally,
the mechanisms by which cancer stem cells regulate glioblastoma progression and
recurrence are described based on novel biomedical technologies. © 2020 Elsevier
Inc.
AN - rayyan-553780247
AU - Bryukhovetskiy, I.
AU - Pak, O.
AU - Khotimchenko, Y.
AU - Bryukhovetskiy, A.
AU - Sharma, A.
AU - Sharma, H. S.
DO - 10.1016/bs.irn.2020.03.002
KW - Blood-brain barrier
Genotoxic therapy
Glioblastoma multiforme
Hematopoietic stem cells
Blood-Brain Barrier
Brain Neoplasms
Glioblastoma
Hematopoietic Stem Cells
Humans
Neoplastic Stem Cells
Precision Medicine
Stem Cell Niche
Tumor Microenvironment
4 hydroxybenzoic acid
bevacizumab
chitosan
cilengitide
cisplatin
doxorubicin
duocarmycin SA
gemcitabine
gold nanoparticle
hyaluronic acid
lomeguatrib
lomustine
macrogol
nanoparticle
olaparib
oncolytic virus
paclitaxel
polyglactin
polylactic acid
procarbazine
rindopepimut
silver nanoparticle
temozolomide
vincristine
vorinostat
blood brain barrier
brain surgery
cancer growth
cancer radiotherapy
cancer recurrence
cancer stem cell
cancer survival
cell interaction
DNA repair
drug cytotoxicity
drug dose increase
drug potentiation
drug resistance
genotoxicity
glioblastoma
human
multiple cycle treatment
nonhuman
personalized medicine
priority journal
proton therapy
stem cell transplantation
brain tumor
hematopoietic stem cell
stem cell niche
Stem Cell Transplantation
PY - 2020
SP - 67-98
ST - Personalized therapy and stem cell transplantation for pro-inflammatory
modulation of cancer stem cells microenvironment in glioblastoma: Review
T2 - International Review of Neurobiology
TI - Personalized therapy and stem cell transplantation for pro-inflammatory
modulation of cancer stem cells microenvironment in glioblastoma: Review
85084631799&doi=10.1016%2fbs.irn.2020.03.002&partnerID=40&md5=45c4dcc50084a827c17f6
b4c7ecf60d9
VL - 151
ID - 8280
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials
due to their antibacterial properties. Owing to the recent boost in the usage of
AgNPs-containing products, human exposure to AgNPs is increasing, highlighting the
need for careful evaluation of AgNPs toxicity in humans. We used two cellular
models, hepatic HepG2 and epithelial A549 cell lines, to study the mechanism of
AgNPs-induced toxicity at the cellular level. These two cell lines differ
significantly in their response to AgNPs treatment. In the case of A549 cells, a
minor decrease in viability and increase in the extent of DNA breakage were
observed. A markedly different response to AgNPs was observed in HepG2 cells. In
short term, a massive induction of DNA breakage was observed, suggesting that the
basal activity of antioxidant defence in these cells was not sufficient to
effectively protect them from the nanoparticle-induced oxidative stress. After
prolonged exposure, the extent of DNA breakage decreased to the level observed in
the control cells proving that a successful adaptation to the new conditions had
taken place. The cells that were unable to adapt must have died, as revealed by the
Neutral Red assay that indicated less than half viable cells after 24-h treatment
with 100 A mu g/ml of 20nm AgNPs. The gene expression analysis revealed that the
observed adaptation was underlain by a pro-proliferative, anti-apoptotic signal
leading to up-regulation of the genes promoting proliferation and inflammatory
response (EGR1, FOS, JUN, HK2, IL4, MMP10, VEGFA, WISP1, CEBPB, IL8, SELPLG), genes
coding the anti-apoptotic proteins (BCL2A1, CCL2) and factors involved in the
response to stress (HSPB1, GADD45A). Such a selection of highly resistant
population of cells should be taken into account in the case of medical
applications of nanoparticles since the sustained proliferative signalling and
resistance to cell death are hallmarks of cancer, acquired by the cells in the
process of carcinogenesis.
AN - rayyan-553780249
AU - Brzoska, K.
AU - Meczynska-Wielgosz, S.
AU - Stepkowski, T. M.
AU - Kruszewski, M.
DO - 10.1093/mutage/gev001
IS - 3
PY - 2015
SN - 0267-8357 1464-3804
SP - 431-439
ST - Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on
the pro-proliferative and anti-apoptotic changes in gene expression
T2 - MUTAGENESIS
TI - Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on
the pro-proliferative and anti-apoptotic changes in gene expression
VL - 30
Y2 - 5
ID - 8281
ER -
TY - JOUR
AB - OBJECTIVE: To evaluate the intraocular reactivity to organic contaminants of
ophthalmic devices in the rabbit. DESIGN: Experimental animal study. PARTICIPANTS:
Fifty New Zealand white rabbits. METHODS: The rabbits were allocated to 10 groups
of 5 each to receive 2 different doses of human albumin and nonhuman nucleic acids
and their respective vehicle controls, a denatured cohesive ophthalmic
viscosurgical device (OVD) and a denatured dispersive OVD and their respective
nondenatured controls. All 10 eyes in each treatment group received bilateral
intracameral injection of the test materials. All the eyes in the study were
examined by slit-lamp biomicroscopy at baseline and 6, 9, 24, 48, and 72 hours.
Pachymetry was also performed on eyes exposed to albumin, protein vehicle control,
and the OVDs at these time points. MAIN OUTCOME MEASURES: Corneal thickness, grade
of corneal clouding, anterior chamber (AC), cells, flare and fibrin, iridal
hyperemia, cell and fibrin on lens surface, and onset time. RESULTS: There were no
inflammatory signs in any eyes exposed to human albumin. Anterior chamber cells (1+
to 3+) and flare and fibrin (1+ to 2+), along with cells and fibrin on the lens
surface, were seen in the eyes exposed to the nucleic acid samples, and they
resolved in 24 hours. Mild (mostly 1+) conjunctival congestion, cells, flare, and
fibrin were seen in a few eyes exposed to the 2 denatured OVDs and their controls,
with the response durations being shorter in the denatured OVD eyes (24 hours) than
in the nondenatured OVD eyes (48 hours). Anterior chamber inflammation was
generally observed in fewer denatured OVD eyes than in nondenatured OVD eyes,
particularly the dispersive OVD eyes. CONCLUSIONS: Intracameral injection of human
albumin protein did not cause ocular inflammation. Nucleic acid intracamerally
injected into rabbit eyes caused acute inflammation that quickly resolved. Cohesive
and dispersive OVD denatured by drying and steam sterilization alone did not cause
ocular inflammation.
AN - rayyan-553782406
AU - Buchen, S. Y.
AU - Calogero, D.
AU - Tarver, M. E.
AU - Hilmantel, G.
AU - Tang, X.
AU - Eydelman, M. B.
DO - 10.1016/j.ophtha.2012.04.007
IS - 7
J2 - Ophthalmology
KW - Albumins/*toxicity
Animals
Anterior Eye Segment/*drug effects
DNA/*toxicity
*Equipment Contamination
Models, Animal
Organic Chemicals/toxicity
RNA, Bacterial/*toxicity
Rabbits
Uveitis, Anterior/*chemically induced
LA - eng
N1 - Food and Drug Administration Oak Ridge Institute for Science and Education
contractor, Silver Spring, Maryland 20993-0002, USA.
PY - 2012
SP - e24-9
ST - Evaluation of intraocular reactivity to organic contaminants of ophthalmic
devices in a rabbit model
T2 - Ophthalmology
TI - Evaluation of intraocular reactivity to organic contaminants of ophthalmic
devices in a rabbit model
VL - 119
Y2 - 7
ID - 10313
ER -
TY - JOUR
AB - Cobalt sulfate is used in the electroplating and electrochemical industries.
It is also used as a coloring agent for ceramics and as a drying agent in inks,
paints, varnishes, and linoleum. Cobalt sulfate may be added to animal feed as a
mineral supplement and has been used as a top dressing on pasture lands. Cobalt
sulfate was nominated by the National Cancer Institute for study based on a lack of
information on the toxicity of soluble salts. Male and female F344/N rats and
B6C3F1 mice were exposed to cobalt sulfate heptahydrate (approximately 99% pure) by
inhalation for 2 years. Genetic toxicology studies were conducted in Salmonella
typhimurium. The results of prechronic inhalation toxicity studies were reported
previously (Bucher et al., 1990; NTP, 1991). 2-YEAR STUDY IN RATS Groups of 50 male
and 50 female rats were exposed to aerosols containing 0, 0.3, 1.0, or 3.0 mg/m3
cobalt sulfate heptahydrate 6 hours per day, 5 days per week, for 105 weeks.
Survival and Body Weights Survival of exposed males and females was similar to that
of the chamber controls. Mean body weights of exposed male and female rats were
similar to those of the chamber controls throughout the study. Pathology Findings
The incidences and severities of proteinosis, alveolar epithelial metaplasia,
granulomatous alveolar inflammation, and interstitial fibrosis were markedly
greater in all exposed groups of male and female rats than in the chamber controls.
The incidences of alveolar epithelial hyperplasia in all groups of exposed males
and in females exposed to 3.0 mg/m3 were significantly greater than those in the
chamber control groups, as were the incidences of squamous metaplasia in 1.0 mg/m3
females and atypical alveolar epithelial hyperplasia in 3.0 mg/m3 females. In 3.0
mg/m3 males, the combined incidence of alveolar/bronchiolar neoplasms (adenoma
and/or carcinoma) was significantly greater than in the chamber controls. In female
rats exposed to 1.0 or 3.0 mg/m3, the incidences of alveolar/bronchiolar neoplasms
were significantly greater than those in the chamber control group and exceeded the
NTP historical control ranges. A squamous cell carcinoma was observed in one 1.0
mg/m3 and one 3.0 mg/m3 female. The incidences of benign, complex, or malignant
pheochromocytoma (combined) in 1.0 mg/m3 males and in 3.0 mg/m3 females were
significantly greater than those in the chamber controls and exceeded the
historical control ranges. Hyperplasia of the lateral wall of the nose, atrophy of
the olfactory epithelium, and squamous metaplasia of the epiglottis were observed
in all exposed groups of males and females, and the severities of these lesions
increased with increasing exposure concentration. The incidences of squamous
metaplasia of the lateral wall of the nose and metaplasia of the olfactory
epithelium were increased in 3.0 mg/m3 males and females. 2-YEAR STUDY IN MICE
Groups of 50 male and 50 female mice were exposed to aerosols containing 0, 0.3,
1.0, or 3.0 mg/m3 cobalt sulfate heptahydrate 6 hours per day, 5 days per week, for
105 weeks. Survival and Body Weights Survival of exposed males and females was
similar to that of the chamber controls. Mean body weights of 3.0 mg/m3 male mice
were less than those of the chamber controls from week 96 until the end of the
study. The mean body weights of all exposed groups of female mice were generally
greater than those of the chamber controls from week 20 until the end of the study.
Pathology Findings The incidences of diffuse histiocytic cell infiltration in 3.0
mg/m3 males and of focal histiocytic cell infiltration in 3.0 mg/m3 females were
significantly greater than those in the chamber controls. The incidences of
alveolar/bronchiolar neoplasms in 3.0 mg/m3 males and females were significantly
greater than those in the chamber control groups. The combined incidences of
alveolar/bronchiolar adenoma or carcinoma and the incidences of
alveolar/bronchiolar carcinoma in 3.0 mg/m3 males and females and the incidence of
alveolar/bronchiolar adenoma in 3.0 mg/m3 females exceeded the NTP historical c
ntrol ranges for inhalation studies. The incidences of atrophy of the olfactory
epithelium in 1.0 and 3.0 mg/m3 males and females and hyperplasia of the olfactory
epithelium in 3.0 mg/m3 males and females were significantly greater than in the
chamber controls. Squamous metaplasia of the larynx was observed in all exposed
groups of males and females. Male mice had a pattern of nonneoplastic liver lesions
along with silver-staining helical organisms within the liver, characteristic of an
infection with Helicobacter hepaticus. In NTP studies with H. hepaticus-associated
hepatitis, increased incidences of heman-giosarcoma were seen in the liver of male
mice. In this study of cobalt sulfate heptahydrate, incidences of hemangiosarcoma
were increased in exposed groups of male mice. Because of the above association,
interpretation of the increased incidences of hemangiosarcoma in the livers of male
mice was confounded. Incidences of lesions at other sites in this study of cobalt
sulfate heptahydrate were not considered to have been significantly impacted by the
infection with H. hepaticus or its associated hepatitis. GENETIC TOXICOLOGY Cobalt
sulfate heptahydrate was mutagenic in S. typhimurium strain TA100 with and without
liver S9 metabolic activation enzymes; no mutagenic activity was detected in strain
TA98 or TA1535, with or without S9. CONCLUSIONS Under the conditions of these 2-
year inhalation studies, there was some evidence of carcinogenic activity* of
cobalt sulfate heptahydrate in male F344/N rats based on increased incidences of
alveolar/bronchiolar neoplasms. Marginal increases in incidences of
pheochromocytomas of the adrenal medulla may have been related to exposure to
cobalt sulfate heptahydrate. There was clear evidence of carcinogenic activity in
female F344/N rats based on increased incidences of alveolar/bronchiolar neoplasms
and pheochromocytomas of the adrenal medulla in groups exposed to cobalt sulfate
heptahydrate. There was clear evidence of carcinogenic activity of cobalt sulfate
heptahydrate in male and female B6C3F1 mice based on increased incidences of
alveolar/bronchiolar neoplasms. Exposure to cobalt sulfate heptahydrate caused a
spectrum of inflammatory, fibrotic, and proliferative lesions in the respiratory
tract of male and female rats and mice. © 1998 NTP Technical Report on the
Toxicology and Carcinogenesis Studies Series. All rights reserved.
AN - rayyan-553780250
AU - Bucher, J. R.
AU - Bridge, D. A.
AU - Chapin, R. E.
AU - Hailey, J. R.
AU - Haseman, J. K.
AU - Maronpot, R. R.
AU - Rao, G. N.
AU - Roycroft, J. H.
AU - Smith, C. S.
AU - Travlos, G. S.
AU - Walters, D. B.
AU - Witt, K. L.
AU - Chou, B. J.
AU - Dill, J. A.
AU - Grumbein, S. L.
AU - Mellick, P. W.
AU - Rowe, S. E.
AU - Hardisty, J. F.
AU - Elwell, M. R.
AU - Shackelford, C. C.
AU - Brecher, S.
AU - Jokinen, M. P.
AU - Dixon, D.
AU - Everitt, J.
AU - Hahn, F. F.
AU - Herbert, R. A.
AU - Nyska, A.
AU - Radovsky, A.
AU - Morris, R. W.
AU - Lloyd, S. R.
AU - Mintz, N. G.
AU - Gunnels, S. R.
AU - Harper, L. M.
AU - Macri-Hanson, A. M.
KW - Rats
Mice
Nebulizers and Vaporizers
PY - 1998
SP - 1-268
ST - NTP TECHNICAL REPORT ON THE TOXICOLOGY AND CARCINOGENESIS STUDIES OF COBALT
SULFATE HEPTAHYDRATE IN F344/N RATS AND B6C3F1 MICE (INHALATION STUDIES)
T2 - NTP Technical Report on the Toxicology and Carcinogenesis Studies Series
TI - NTP TECHNICAL REPORT ON THE TOXICOLOGY AND CARCINOGENESIS STUDIES OF COBALT
SULFATE HEPTAHYDRATE IN F344/N RATS AND B6C3F1 MICE (INHALATION STUDIES)
85149251503&partnerID=40&md5=e78a2a3f4a4739f31ff39a94b8f67487
ID - 8282
ER -
TY - JOUR
AB - The common effects of acetaminophen (APA), isopropylantipyrine (IPA) and
caffeine on liver were examined in rats. The preparations were given in Tween-80
solution once daily, in a constant proportion of 5:3:1, during days 8 to 14 of
gestation (S1, S2, S3 groups) or between days 8 and 14 of the experiment in
nonpregnant female Wistar rats (S1-NP, S2-NP, S3-NP groups). The administration was
in three different doses: doses S1, S1-NP - 3.5 mg/kg APA, 2.14 mg/kg IPA, 0.7
mg/kg caffeine; doses S2, S2-NP were 10 times higher; and doses S3, S3-NP 100 times
higher than doses S1, S1-NP. There were two control groups (T, T-NP) with Tween-80.
At day 21 of gestation/experiment blood was taken for determination of activity of
alanine (ALA) and aspartate (AST) aminotransferase, lactate (LDH) and glutamate
(GLDH) dehydrogenase, levels of bilirubin (BIL), urea (URE), total protein (TP) and
thymol turbidity test (TTT). The liver sections were examined by light microscopy
with four stains: hematoxylin and eosin (H+E), silver Gomori, van Gieson and
periodic acid-Schiff (PAS). There was a statistically significant (P<0.05) increase
in the GLDH (S3-NP, S2, S3 groups) and AST, LDH (S3 group) activity, a decrease in
URE (S2, S3 groups) and decrease in TP (S3-NP, S2, S3 groups) compared to the
corresponding control group. Significant differences were noted in activity of AST,
GLDH, and levels of the URE and TP between pregnant and nonpregnant females. The
treatment resulted in minimal reactive and degenerative changes in light
microscopic pattern of liver.
AN - rayyan-553780253
AU - Burdan, F.
DO - 10.1191/096032701718620873
IS - 11
KW - Acetaminophen
Caffeine
Isopropylantipyrine
Liver
Paracetamol
Pregnancy
Propyphenazone
Rat
Toxic injury
Analgesics, Non-Narcotic
Animals
Anti-Inflammatory Agents, Non-Steroidal
Antipyrine
Body Weight
Central Nervous System Stimulants
Drug Combinations
Female
Hepatitis, Toxic
Liver Function Tests
Organ Size
Periodic Acid-Schiff Reaction
Pregnancy, Animal
Rats
Rats, Wistar
Animalia
Rattus norvegicus
bilirubin
caffeine
eosin
glutamate dehydrogenase
hematoxylin
paracetamol
propyphenazone
protein
thymol
urea
animal experiment
animal model
animal tissue
article
aspartate aminotransferase blood level
blood analysis
controlled study
histopathology
laboratory test
lactate dehydrogenase blood level
liver biopsy
liver injury
liver toxicity
microscopy
nonhuman
periodic acid Schiff stain
pregnancy
priority journal
rat
statistical analysis
urea nitrogen blood level
PY - 2001
SP - 569-575
ST - Combined effects of acetaminophen, isopropylantipyrine and caffeine on
pregnant and nonpregnant liver
T2 - Human and Experimental Toxicology
TI - Combined effects of acetaminophen, isopropylantipyrine and caffeine on
pregnant and nonpregnant liver
0035724974&doi=10.1191%2f096032701718620873&partnerID=40&md5=25c18a9a9f4958a9e05c55
becd7e0212
VL - 20
ID - 8285
ER -
TY - JOUR
AB - During the past few years, silver nanoparticles (AgNPs) became one of the
most investigated and explored nanotechnology-derived nanostructures, given the
fact that nanosilver-based materials proved to have interesting, challenging, and
promising characteristics suitable for various biomedical applications. Among
modern biomedical potential of AgNPs, tremendous interest is oriented toward the
therapeutically enhanced personalized healthcare practice. AgNPs proved to have
genuine features and impressive potential for the development of novel
antimicrobial agents, drug-delivery formulations, detection and diagnosis
platforms, biomaterial and medical device coatings, tissue restoration and
regeneration materials, complex healthcare condition strategies, and performance-
enhanced therapeutic alternatives. Given the impressive biomedical-related
potential applications of AgNPs, impressive efforts were undertaken on
understanding the intricate mechanisms of their biological interactions and
possible toxic effects. Within this review, we focused on the latest data regarding
the biomedical use of AgNP-based nanostructures, including aspects related to their
potential toxicity, unique physiochemical properties, and biofunctional behaviors,
discussing herein the intrinsic anti-inflammatory, antibacterial, antiviral, and
antifungal activities of silver-based nanostructures.
AN - rayyan-553780255
AU - Burdusel, A. C.
AU - Gherasim, O.
AU - Grumezescu, A. M.
AU - Mogoanta, L.
AU - Ficai, A.
AU - Andronescu, E.
DO - 10.3390/nano8090681
IS - 9
PY - 2018
SN - 2079-4991
ST - Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview
T2 - NANOMATERIALS
TI - Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview
VL - 8
Y2 - 9
ID - 8287
ER -
TY - JOUR
AB - During the past few years, silver nanoparticles (AgNPs) became one of the
most investigated and explored nanotechnology-derived nanostructures, given the
fact that nanosilver-based materials proved to have interesting, challenging, and
promising characteristics suitable for various biomedical applications. Among
modern biomedical potential of AgNPs, tremendous interest is oriented toward the
therapeutically enhanced personalized healthcare practice. AgNPs proved to have
genuine features and impressive potential for the development of novel
antimicrobial agents, drug-delivery formulations, detection and diagnosis
platforms, biomaterial and medical device coatings, tissue restoration and
regeneration materials, complex healthcare condition strategies, and performance-
enhanced therapeutic alternatives. Given the impressive biomedical-related
potential applications of AgNPs, impressive efforts were undertaken on
understanding the intricate mechanisms of their biological interactions and
possible toxic effects. Within this review, we focused on the latest data regarding
the biomedical use of AgNP-based nanostructures, including aspects related to their
potential toxicity, unique physiochemical properties, and biofunctional behaviors,
discussing herein the intrinsic anti-inflammatory, antibacterial, antiviral, and
antifungal activities of silver-based nanostructures.
AN - rayyan-553781778
AU - Burdușel, A. C.
AU - Gherasim, O.
AU - Mogoantă, L.
AU - Ficai, A.
AU - Andronescu, E.
DO - 10.3390/nano8090681
IS - 9
J2 - Nanomaterials (Basel)
LA - eng
N1 - Faculty of Engineering in Foreign Languages, University Politehnica of
Bucharest, 313 Splaiul Independenței, Bucharest 060042, Romania.
alexandra_burdu@yahoo.com.sg.; Department of Science and Engineering of Oxide
Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science,
University Politehnica of Bucharest, 1-7 Gheorghe Polizu Street, Bucharest 011061,
Romania. oana.fufa@gmail.com.; Lasers Department, National Institute for Lasers,
Plasma and Radiation Physics, 409 Atomiștilor Street, Magurele 077125, Romania.
oana.fufa@gmail.com.; Department of Science and Engineering of Oxide Materials and
Nanomaterials, Faculty of Applied Chemistry and Materials Science, University
Politehnica of Bucharest, 1-7 Gheorghe Polizu Street, Bucharest 011061, Romania.
grumezescu@yahoo.com.; Research Center for Microscopic Morphology and Immunology,
University of Medicine and Pharmacy of Craiova, 2 Petru Rareș Street, Craiova
200349, Romania. laurentiu_mogoanta@yahoo.com.; Department of Science and
Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and
Materials Science, University Politehnica of Bucharest, 1-7 Gheorghe Polizu Street,
Bucharest 011061, Romania. anton.ficai@upb.ro.; Department of Science and
Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and
Materials Science, University Politehnica of Bucharest, 1-7 Gheorghe Polizu Street,
Bucharest 011061, Romania. ecaterina.andronescu@upb.ro.
PY - 2018
SN - 2079-4991 (Print)
ST - Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview
T2 - Nanomaterials (Basel, Switzerland)
TI - Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview
VL - 8
Y2 - 8 y3 - 31
ID - 9709
ER -
TY - JOUR
AB - Herein, a new silver(I) complex with the non-steroidal anti-inflammatory drug
naproxen and nitrogen donor 3-picoline ligands was synthesized, characterized, and
subsequently tested for its cytotoxicity against different types of cancer cell
lines. Elemental analysis, Fourier transform infrared spectroscopy, thermal, and
proton nuclear magnetic resonance techniques showed that the molecular formula of
the prepared complex is bis(3-picoline)tris(μ-naproxenato)trisilver(I) and naproxen
ligands bind to silver ions in a bridging bidentate mode. 2,3-bis-(2-methoxy-4-
nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) results revealed that
silver salts and naproxen alone showed quite weak cytotoxic activity against human
breast adenocarcinoma (MDA-MB-453), lung adenocarcinoma (A-549), and colorectal
adenocarcinoma (HT-29) cell lines (IC50 > 250 µM), whereas the complex displayed
dose dependent cytotoxicity against the aforementioned cell lines. The highest
cytotoxicity was observed on MDA-MB-453 cells with an IC50 value of 11.73 µM.
Moreover, the complex showed higher selectivity against the cancer cell lines
compared to fibroblast 3T3-L1 cells. This study provides preliminary scientific
data on the complex for further elucidation of its anticancer mechanism of action.
© 2021
AN - rayyan-553780257
AU - Caglar, S.
AU - Altay, A.
AU - Harurluoğlu, B.
AU - Caglar, B.
DO - 10.20450/MJCCE.2021.2414
IS - 2
KW - 3-picoline
cell culture
cytotoxicity
naproxen
Silver(I) complex
Naproxen
PY - 2021
SP - 171-180
ST - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
NAPROXEN: SYNTHESIS, CHARACTERIZATION, AND IN VITRO CYTOTOXICITY
T2 - Macedonian Journal of Chemistry and Chemical Engineering
TI - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
85123805078&doi=10.20450%2fMJCCE.2021.2414&partnerID=40&md5=69aaf13bce6a141e1b9227d
bb15ab1fc
VL - 40
ID - 8289
ER -
TY - JOUR
AB - Herein, a new silver(I) complex with the non-steroidal anti-inflammatory drug
naproxen and nitrogen donor 3-picoline ligands was synthesized, characterized, and
subsequently tested for its cytotoxicity against different types of cancer cell
lines. Elemental analysis, Fourier transform infrared spectroscopy, thermal, and
proton nuclear magnetic resonance techniques showed that the molecular formula of
the prepared complex is bis(3-picoline)tris(mu-naproxenato)trisilver(I) and
naproxen ligands bind to silver ions in a bridging bidentate mode. 2,3-bis-(2-
methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) results
revealed that silver salts and naproxen alone showed quite weak cytotoxic activity
against human breast adenocarcinoma (MDA-MB-453), lung adenocarcinoma (A-549), and
colorectal adenocarcinoma (HT-29) cell lines (IC50 > 250 mu M), whereas the complex
displayed dose dependent cytotoxicity against the aforementioned cell lines. The
highest cytotoxicity was observed on MDA-MB-453 cells with an IC50 value of 11.73
mu M. Moreover, the complex showed higher selectivity against the cancer cell lines
compared to fibroblast 3T3-L1 cells. This study provides preliminary scientific
data on the complex for further elucidation of its anticancer mechanism of action.
AN - rayyan-553780258
AU - Caglar, S.
AU - Altay, A.
AU - Harurluoglu, B.
AU - Caglar, B.
DO - 10.20450/mjcce.2021.2414
IS - 2
KW - Naproxen
PY - 2021
SN - 1857-5552 1857-5625
SP - 171-180
ST - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
T2 - MACEDONIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING
TI - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
VL - 40
ID - 8290
ER -
TY - JOUR
AB - Herein, we report the synthesis, characterization and anticancer activity of
six novel complexes of non-steroidal anti-inflammatory drug niflumic acid with
Co(II) and Ni(II). In vitro cytotoxicity screening in MCF-7, HepG2 and HT-29 cancer
cell lines showed that the complex 3 [Co(nif)(2)(met)(4-pic)] and complex 6
[Ni(nif)(2)(met)(4-pic)] among all the complexes exhibited the highest cytotoxicity
against MCF-7 cells with IC50 values of 11.14 mu M and, 41.47 mu M, respectively.
Besides, all the complexes exhibited significantly higher selectivity towards mouse
fibroblast 3T3L1 cells. Further mechanistic studies with both complexes on MCF-7
cells revealed their cytotoxic action through the mitochondrial-dependent apoptotic
pathway causing an increase oxidative/nitrosative stress, decrease in mitochondrial
membrane potential (Delta psi m), inducing the multicaspase activation and
arresting the cell cycle at S phase. q-PCR analysis resulted in an increase in the
expression of the apoptotic marker proteins bax, p53 and caspase-3 and -8 in MCF-7
cells, but a decrease in the expression of antiapoptotic bcl-2 gene. Moreover, both
complexes induced the apoptosis through the inhibition of PI3K/Akt signaling
pathway by decreasing the expression of PI3K and increasing dephosphorylation form
of Akt protein. These results provide a significant contribution to the explanation
of the anticancer mechanisms of these complexes in MCF-7 cells.
AN - rayyan-553780259
AU - Caglar, S.
AU - Altay, A.
AU - Kuzucu, M.
AU - Caglar, B.
DO - 10.1007/s12013-021-00984-z
IS - 4
KW - Humanities
Humanism
Humans
Adenocarcinoma
PY - 2021
SN - 1085-9195 1559-0283
SP - 729-746
ST - In Vitro Anticancer Activity of Novel Co(II) and Ni(II) Complexes of Non-
steroidal Anti-inflammatory Drug Niflumic Acid Against Human Breast Adenocarcinoma
MCF-7 Cells
T2 - CELL BIOCHEMISTRY AND BIOPHYSICS
TI - In Vitro Anticancer Activity of Novel Co(II) and Ni(II) Complexes of Non-
steroidal Anti-inflammatory Drug Niflumic Acid Against Human Breast Adenocarcinoma
MCF-7 Cells
VL - 79
Y2 - 12
ID - 8291
ER -
TY - JOUR
AB - Immune responses are involved in the pathogenesis of many diseases, including
cancer, autoimmune diseases, and chronic inflammation. These responses are
attributed to immune cells that produce cytokines, mediate cytotoxicity, and
synthesize antibodies. Gold nanoparticles (GNPs) are novel agents that intervene
with immune responses because of their unique physical-chemical properties. In
particular, GNPs enhance anti-tumour activity during immunotherapy and eliminate
excessive inflammation in autoimmune diseases. However, GNPs synthesized by
conventional methods are toxic to living organisms. Green biosynthesis provides a
safe and eco-friendly method to obtain GNPs from microbes or plant extracts. In
this review, we describe several patterns for green GNP biosynthesis. The
applications of GNPs to target immune cells and modulate the immune response are
summarized. In particular, we elaborate on how GNPs regulate innate immunity and
adaptive immunity, including inflammatory signaling and immune cell
differentiation. Finally, perspectives and challenges in utilizing green
biosynthesized GNPs for novel therapeutic approaches are discussed.
AN - rayyan-553780260
AU - Cai, F. Y.
AU - Li, S. Y.
AU - Huang, H.
AU - Iqbal, J.
AU - Wang, C. R.
AU - Jiang, X.
DO - 10.1002/jbm.a.37281
PY - 2021
SN - 1549-3296 1552-4965
ST - Green synthesis of gold nanoparticles for immune response regulation:
Mechanisms, applications, and perspectives
T2 - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
TI - Green synthesis of gold nanoparticles for immune response regulation:
Mechanisms, applications, and perspectives
Y2 - 7 y3 - 31
ID - 8292
ER -
TY - JOUR
AB - Design of micro- or nanocarriers for drug delivery has primarily been focused
on properties such as hydrophobicity, biodegradability, size, shape, surface
charge, and toxicity, so that they can achieve optimal delivery with respect to
drug loading, release kinetics, biodistribution, cellular uptake, and
biocompatibility. Incorporation of stimulus-sensitive moieties into the carriers
would lead to “smart” delivery systems. A further evolution would be to endow the
carrier with a therapeutic function such that it no longer serves as a mere passive
entity to release the drug at the target tissue but can be viewed as a therapeutic
agent in itself. In this review, we will discuss recent and ongoing efforts over
the past decade to design therapeutic drug carriers that confer a biological
benefit, including ROS scavenging or generating, pro- or anti-inflammatory, and
immuno-evasive properties, to enhance the overall therapeutic efficacy of the
delivery systems. © 2021 Elsevier B.V.
AN - rayyan-553780261
AU - Cai, S. S.
AU - Li, T.
AU - Akinade, T.
AU - Zhu, Y.
AU - Leong, K. W.
DO - 10.1016/j.addr.2021.113884
KW - Anti-bacterial
Anti-fibrotic
Anti-inflammatory
Anti-microbial
Drug delivery
Immunoadjuvant
ROS generation
ROS scavenging
Therapeutic carriers
Animals
Drug Delivery Systems
Humans
Biocompatibility
Biodegradability
Biology
chitosan
dendrimer
gold nanoparticle
hyaluronic acid
manganese
polyglactin
silver nanoparticle
Anti-inflammatories
Delivery systems
Drug delivery carrier
Stimulus-sensitive
Therapeutic agents
Therapeutic drugs
Therapeutic efficacy
Therapeutic functions
Alzheimer disease
apoptosis
autoimmune disease
biosafety
cancer chemotherapy
cancer radiotherapy
cytotoxicity
drug mechanism
immunomodulation
micelle
molecular weight
nanocatalyst
nanofabrication
nonhuman
oxidative stress
particle size
photothermal therapy
Review
signal transduction
animal
human
PY - 2021
ST - Drug delivery carriers with therapeutic functions
T2 - Advanced Drug Delivery Reviews
TI - Drug delivery carriers with therapeutic functions
85111542782&doi=10.1016%2fj.addr.2021.113884&partnerID=40&md5=8f2121fa126842a2993a2
3e290d095ed
VL - 176
ID - 8293
ER -
TY - JOUR
AB - OBJECTIVE: To evaluate the intraocular reactivity to metallic and ethylene
oxide (EO) contaminants of ophthalmic devices in rabbits. DESIGN: Two experimental
animal studies. PARTICIPANTS: Thirty-five New Zealand white rabbits. METHODS: A
metallic exposure study and an EO exposure study were performed. In the first
study, both eyes of 25 rabbits were equally allocated to intracameral injections of
alumina 0.2 μg, alumina 20 μg, copper sulfate 0.4 μg, copper sulfate 20 μg, or an
aqueous control. In the second study, 10 rabbits were allocated (5 per group) to
receive intracamerally an ophthalmic viscosurgical device (OVD) exposed to EO or
not exposed to EO (control). All eyes were examined by slit lamp at baseline and 3,
6, 9, 24, 48, and 72 hours after exposure, with dilated indirect ophthalmoscopy
being performed at 24 and 72 hours. Tonometry was performed only in the first
study. MAIN OUTCOME MEASURES: Grade of corneal clouding, anterior chamber (AC)
flare, AC cells, AC fibrin, iridal hyperemia, cell and fibrin on the lens surface,
vitreous haze and cells, lens opacities, intraocular pressure, and onset time.
RESULTS: For metallic compounds at the study's low doses, mean inflammatory grades
were 0.2 or less above the control for all responses at all time points. For the
high-dose alumina, mean inflammatory grades peaked at 6 to 9 hours at 0.5 to 0.7
above the control responses for conjunctival congestion, iris hyperemia, AC cells,
flare, and fibrin and declined over the remaining time points. For the high-dose
copper sulfate, mean inflammatory grades peaked between 3 and 24 hours at 1.2 to
1.8 above the control responses for conjunctival congestion, iris hyperemia, AC
cells, flare, fibrin, and corneal clouding, then subsequently declined. The
intraocular pressure changes appeared significant for only high-dose copper
sulfate, with mean declines of 4.3 to 7.5 mmHg at 6 to 72 hours. No clinically
meaningful differences in ocular inflammation were observed between the OVD exposed
to EO and the OVD not exposed to EO. CONCLUSIONS: Alumina and copper sulfate did
not cause clinically meaningful ocular inflammation at the low study levels (levels
expected with ophthalmic devices). Ethylene oxide exposure of an OVD was not
associated with inflammation.
AN - rayyan-553782407
AU - Calogero, D.
AU - Buchen, S. Y.
AU - Tarver, M. E.
AU - Hilmantel, G.
AU - Lucas, A. D.
AU - Eydelman, M. B.
DO - 10.1016/j.ophtha.2012.04.009
IS - 7
J2 - Ophthalmology
KW - Aluminum Oxide/*toxicity
Animals
Anterior Eye Segment/*drug effects
Copper Sulfate/*toxicity
*Equipment Contamination
Ethylene Oxide/*toxicity
Models, Animal
Ophthalmologic Surgical Procedures/*instrumentation
Rabbits
Uveitis, Anterior/*chemically induced/diagnosis
LA - eng
N1 - Food and Drug Administration, Center for Devices and Radiological Health,
Office of Device Evaluation, Division of Ophthalmic, Neurological, and Ear, Nose,
and Throat Devices, Silver Spring, Maryland 20993-0002, USA.
PY - 2012
SP - e36-42
ST - Evaluation of intraocular reactivity to metallic and ethylene oxide
contaminants of medical devices in a rabbit model
T2 - Ophthalmology
TI - Evaluation of intraocular reactivity to metallic and ethylene oxide
contaminants of medical devices in a rabbit model
VL - 119
Y2 - 7
ID - 10314
ER -
TY - JOUR
AN - rayyan-553782254
AU - Calura, G.
AU - Nonato, M.
AU - Mazzoli, S.
AU - Berti, G.
AU - Ventrelli, I.
IS - 5
J2 - Mondo Odontostomatol
Animals
Bisphenol A-Glycidyl Methacrylate
Dental Amalgam/pharmacology
Dexamethasone/pharmacology
Drug Combinations/pharmacology
Formaldehyde/pharmacology
Gutta-Percha/pharmacology
*Hydrocortisone
Polymethacrylic Acids/pharmacology
Rats
Root Canal Filling Materials/*pharmacology
Silver/pharmacology
Skin/anatomy & histology/drug effects
Thymol/analogs & derivatives/pharmacology
LA - ita
PY - 1984
SN - 0026-9565 (Print)
SP - 43-8
ST - [Histologic evaluation of the characteristics of tissue biocompatibility of 4
materials used in endodontics]
T2 - Mondo odontostomatologico
TI - [Histologic evaluation of the characteristics of tissue biocompatibility of 4
materials used in endodontics]
VL - 26
Y2 - 9
ID - 10164
ER -
TY - JOUR
AB - Few studies were made regarding the pulmonary effects of exposure to
volcanogenic air pollution, representing an unrecognized health risk for humans
inhabiting non-eruptive volcanically active areas (10% of world human population).
We tested the hypothesis whether chronic exposure to air pollution of volcanogenic
origin causes lung injury, using wild mice (Mus musculus) as model. Lung injury was
determined using histological morphometric parameters, inflammatory status (InfS)
and the amount of black silver deposits (BSD). Mice exposed to volcanogenic air
pollution have decreased percentage of alveolar space, alveolar perimeter and lung
structural functionality (LSF) ratio and, increased alveolar septal thickness,
amount of BSD and InfS. For the first time it is evidenced that non-eruptive active
volcanism has a high potential to cause lung injury. This study also highlights the
usefulness of M. musculus as bioindicator species, and of the developed biomarker
of effect LSF ratio, for future animal and/or human biomonitoring programs. © 2013
Elsevier Ltd. All rights reserved.
AN - rayyan-553780262
AU - Camarinho, R.
AU - Garcia, P. V.
AU - Rodrigues, A. S.
DO - 10.1016/j.envpol.2013.05.052
KW - Air pollution
Lung structural functionality ratio
Mice
Pulmonary inflammation
Volcanism
Air Pollutants
Air Pollution
Animals
Environmental Monitoring
Humans
Inhalation Exposure
Lung
Lung Injury
Toxicity Tests, Chronic
Volcanic Eruptions
Animalia
Mus
Mus musculus
Biomarkers
Mammals
Silver deposits
Volcanoes
Alveolar Perimeter
Alveolar Septal Thickness
AlvP
AlvST
Black Silver Deposits
BSD
Inflammatory Status
InfS
LSF ratio
Lung Structural Functionality ratio
PAlvS
Percentage of Alveolar Space
Bioindicator species
Chronic exposure
Human biomonitoring
Morphometric parameters
Structural functionality
atmospheric pollution
bioindicator
health risk
hypothesis testing
injury
pollution exposure
respiratory disease
rodent
volcanism
air pollutant
air pollution
air pollution indicator
animal experiment
animal model
animal tissue
article
black silver deposit
histopathology
lung alveolus
lung function
lung injury
mouse
nonhuman
pneumonia
tissue structure
volcano
volcanogenic air pollution
wild animal
Biological organs
PY - 2013
SP - 24-30
ST - Chronic exposure to volcanogenic air pollution as cause of lung injury
T2 - Environmental Pollution
TI - Chronic exposure to volcanogenic air pollution as cause of lung injury
84879427233&doi=10.1016%2fj.envpol.2013.05.052&partnerID=40&md5=22944d5b6c613a70ab0
2d05bd90ddfb9
VL - 181
ID - 8294
ER -
TY - JOUR
AB - With the rapid development of nanotechnologies, nanoparticles (NPs) are
increasingly produced and used in many commercial products, which could lead to the
contact of human blood vessels with NPs. Thus, it is necessary to understand the
adverse effects of NPs to relevant cells lining human blood vessels, especially
endothelial cells (ECs) that cover the lumen of blood vessels. Human umbilical vein
endothelial cells (HUVECs) are among one of the most popular models used for ECs in
vitro. In the present review, we discussed studies that have used HUVECs as a model
to investigate the EC-NP interactions, the toxic effects of NPs on ECs and the
mechanisms. The results of these studies indicated that NPs could be internalized
into HUVECs by the endocytosis pathway as well as transported across HUVECs by
exocytosis and paracellular pathways. Exposure of HUVECs to NPs could induce
cytotoxicity, genotoxicity, eNOS uncoupling and endothelial activation, which could
be explained by NP-induced oxidative stress, inflammatory response and dysfunction
of organelles. In addition, some studies have also evaluated the influences of
microenvironment (e.g. the presence of proteins and excessive nutrients), the
physiological and/or pathological stimuli related to the diversity of ECs (e.g.
shear stress, cyclic stretch and inflammatory stimuli), and the physicochemical
properties of NPs on the responses of ECs to NP exposure. In conclusion, it has
been suggested that HUVECs could be considered as a relatively reliable and simple
in vitro model for ECs to predict and evaluate the toxicity of NPs to endothelium.
Copyright (c) 2017 John Wiley & Sons, Ltd. The increasing uses of nanoparticles
(NPs) could lead to contact of human blood vessels to NPs, and it is necessary to
assess the toxicity to endothelial cells (ECs) covering blood vessels. Human
umbilical vein endothelial cells (HUVECs) are among one of the most used models for
ECs. In this review, we discuss the validation of HUVECs as a model for ECs, the
NP-HUVEC interaction, the toxic effects of NP to HUVECs, and the mechanisms and
factors that can influence the responses.
AN - rayyan-553780266
AU - Cao, Y.
AU - Gong, Y.
AU - Liu, L. L.
AU - Zhou, Y. W.
AU - Fang, X.
AU - Zhang, C.
AU - Li, Y. N.
AU - Li, J.
DO - 10.1002/jat.3470
IS - 12
KW - Humanities
Humanism
Humans
PY - 2017
SN - 0260-437X 1099-1263
SP - 1359-1369
ST - The use of human umbilical vein endothelial cells (HUVECs) as an in vitro
model to assess the toxicity of nanoparticles to endothelium: a review
TI - The use of human umbilical vein endothelial cells (HUVECs) as an in vitro
model to assess the toxicity of nanoparticles to endothelium: a review
VL - 37
Y2 - 12
ID - 8298
ER -
TY - JOUR
AB - Nickel oxide nanoparticles (NiONPs) toxicity has been evaluated in the human
pulmonary epithelial cell lines: BEAS-2B and A549. The nanoparticles, used at the
doses of 20, 40, 60, 80, 100 mu g/ml, induced a significant reduction of cell
viability and an increase of apoptotic and necrotic cells at 24 h. A significant
release of interleukin-6 and -8 was assessed after 24h of treatment, even
intracellular ROS increased already at 45 min after exposure. The results obtained
evidenced that the cytokines release was dependent on mitogen activated protein
kinases (MAPK) cascade through the induction of NF-kB pathway. NiONPs induced cell
cycle alteration in both the cell lines even in different phases and these
modifications may be induced by the NPs genotoxic effect, suggested by the nuclear
translocation of phospho-ATM and phospho-ATR. Our results confirm the cytotoxic and
pro-inflammatory potential of NiONPs. Moreover their ability in inducing DNA damage
responses has been demonstrated. Such effects were present in A549 cells which
internalize the NPs and BEAS-2B cells in which endocytosis has not been observed.
(C) 2014 Elsevier Ireland Ltd. All rights reserved.
AN - rayyan-553780267
AU - Capasso, L.
AU - Camatini, M.
AU - Gualtieri, M.
DO - 10.1016/j.toxlet.2014.01.040
IS - 1
KW - Nickel
Epithelial Cells
Lung
Inflammation
PY - 2014
SN - 0378-4274 1879-3169
SP - 28-34
ST - Nickel oxide nanoparticles induce inflammation and genotoxic effect in lung
epithelial cells
T2 - TOXICOLOGY LETTERS
TI - Nickel oxide nanoparticles induce inflammation and genotoxic effect in lung
epithelial cells
VL - 226
Y2 - 4 y3 - 7
ID - 8299
ER -
TY - JOUR
AB - Background and objects: Lipids with platelet activating factor (PAF)-like
activity in supernatant of packed red blood cells (PRBC) cause priming of the
neutrophil respiratory burst. This effect increases with length of storage. Washing
of PRBC has been considered as a means to eliminate this effect; however, the role
of the cellular component was not evaluated independently of the supernatant. The
source of the inflammatory lipids of the supernatant is likely to be cell membranes
altered during ageing in storage and therefore, washing will not eliminate
neutrophil priming caused by transfusion of aged PRBC units. The ability of washed
PRBC to prime mononuclear cells for another known effect of PAF, the production of
IL-8, and the probability that this lipid activity is present on microparticles in
PRBC supernatant were also investigated. Materials and methods: At collection 10
units of whole blood were split into two equal aliquots one filtered and one
unfiltered. PRBC were prepared and stored at 4 degrees C in CPD-AS5. Each week,
fresh neutrophils were incubated with samples of washed PRBC and fixed. Change in
CD11b, a marker known to increase on the surface of primed neutrophils, was
determined by flow cytometry. To determine whether neutrophil priming ability of
PRBC supernatant is contained on microvesicles, centrifuged and uncentrifuged
supernatant samples were incubated with fresh neutrophils and change in CD11b
expression was determined. Plasma IL-8 levels were also measured after exposure of
monocytes from fresh whole blood to filtered and unfiltered washed PRBC with and
without the addition of fMLP. Results: Washed PRBC caused a 50-116% increase in
CD11b neutrophil surface expression over baseline expression. Filtration of whole
blood at collection reduced this CD11b up-regulation by 25-34%. Reduction of
priming ability by filtration began on the day of collection and persisted for the
storage life of the units. Centrifugation resulted in a reduction of CD11b up-
regulation of 11-28% compared with unspun supernatant. Incubation of unfiltered
PRBC resulted in priming of mononuclear leukocytes for IL-8 production with a 73-
109% increase over baseline, but no increase over baseline was seen for incubation
with filtered blood. Conclusion: Washing does not eliminate the ability of PRBC
units to prime neutrophils and mononuclear cells, because the cellular component of
PRBC, in addition to the supernatant, induces priming. Leukodepletion filters
significantly reduce these effects compared with unfiltered PRBC. The in vitro
beneficial effect of filtration lasts for the shelf life of 42 day units. The
ability of PRBC supernatant to prime neutrophils is present on microvesicles.
Published by Elsevier Ltd.
AN - rayyan-553780268
AU - Cardo, L. J.
AU - Wilder, D.
AU - Salata, J.
DO - 10.1016/j.transci.2008.01.004
IS - 2
PY - 2008
SN - 1473-0502
SP - 117-125
ST - Neutrophil priming, caused by cell membranes and microvesicles in packed red
blood cell units, is abrogated by leukocyte depletion at collection
T2 - TRANSFUSION AND APHERESIS SCIENCE
TI - Neutrophil priming, caused by cell membranes and microvesicles in packed red
blood cell units, is abrogated by leukocyte depletion at collection
VL - 38
Y2 - 4
ID - 8300
ER -
TY - JOUR
AB - The indusium griseum (IG), a thin layer of gray matter in contact with the
dorsal surface of the corpus callosum and the lateral gray matter of the cingulate
gyrus, has a common origin with hippocampus and shows similar organization with the
dentate gyrus. Although some studies have examined the effect of methamphetamine
(METH), an addictive and an illegal psychostimulant on this structure, quantitative
effects and possible mechanism of actions of METH in this area are lacking. By
applying two different protocols of equivalent METH administration (i.e., a high
dose of 1 × 30 mg/kg and a lower and repeated injection dose of 3 × 10 mg/kg) and
using a specific silver staining method in mice, we demonstrate that this drug
produces degeneration in IG with both protocols, without affecting the dopaminergic
system. Moreover, we observed quantitative increases in labeling of GFAP and Iba-1,
markers of astro- and microgliosis, respectively, which suggest astrogliosis and
microgliosis. Thus, our study provides morphological and semi-quantitative evidence
that METH induces neurodegeneration in IG and that this damage is associated with
astrogliosis and microgliosis in this area. © 2014, Springer Science+Business Media
New York.
AN - rayyan-553780271
AU - Carmena, A.
AU - Granado, N.
AU - Ares-Santos, S.
AU - Alberquilla, S.
AU - Tizabi, Y.
AU - Moratalla, R.
DO - 10.1007/s12640-014-9505-9
IS - 3
KW - Astrogliosis
Indusium griseum
Methamphetamine
Microgliosis
Neurodegeneration
Silver Staining
Animals
Astrocytes
Gliosis
Limbic Lobe
Male
Mice
Mice, Inbred C57BL
Microglia
allograft inflammatory factor 1
biological marker
methamphetamine
tyrosine 3 monooxygenase
animal experiment
animal model
animal tissue
Article
astrocytosis
brain region
controlled study
corpus striatum
dopaminergic nerve cell
dopaminergic system
gliosis
hyperthermia
indusium griseum
male
mouse
nerve degeneration
nonhuman
priority journal
quantitative analysis
rectum temperature
silver staining
toxicology
animal
astrocyte
C57BL mouse
chemically induced
drug effects
limbic cortex
microglia
pathology
PY - 2015
SP - 209-216
ST - Methamphetamine-Induced Toxicity in Indusium Griseum of Mice is Associated
with Astro- and Microgliosis
T2 - Neurotoxicity Research
TI - Methamphetamine-Induced Toxicity in Indusium Griseum of Mice is Associated
with Astro- and Microgliosis
84925464640&doi=10.1007%2fs12640-014-9505-
9&partnerID=40&md5=deb9739a049e4bb6a5481514ac081f17
VL - 27
ID - 8303
ER -
TY - JOUR
AB - This study employed NMR metabolomics to characterize macrophage responses to
subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver
(Ag+), with a view to further elucidate their immunomodulatory activity at the cell
metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease
intracellular glucose utilization, possibly due to interference with glycolytic
enzymes, and to reprogram the TCA cycle towards anaplerotic fueling and production
of anti-inflammatory metabolites (e.g. itaconate and creatine). Moreover, AgNPs-
exposed cells were able to control the levels of reactive oxygen/nitrogen species
(ROS/RNS), likely through upregulation of glutathione synthesis. On the other hand,
macrophages exposed to Ag+ at equivalent subtoxic concentrations showed reduced
metabolic activity, lower ability to counterbalance ROS/RNS and alterations in
membrane-related lipids. Overall, the metabolomics approach hereby employed
provided novel insights into the differential effects of AgNPs and Ag+, which help
explain the lower toxic potential of nanosilver than silver ions.
AN - rayyan-553780272
AU - Carrola, J.
AU - Bastos, V.
AU - Daniel-da-Silva, A. L.
AU - Gil, A. M.
AU - Santos, C.
AU - Oliveira, H.
AU - Duarte, I. F.
DO - 10.1002/ejic.202000095
IS - 19
KW - Macrophages
PY - 2020
SN - 1434-1948 1099-0682
SP - 1867-1876
ST - Macrophage Metabolomics Reveals Differential Metabolic Responses to Subtoxic
Levels of Silver Nanoparticles and Ionic Silver
T2 - EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
TI - Macrophage Metabolomics Reveals Differential Metabolic Responses to Subtoxic
Levels of Silver Nanoparticles and Ionic Silver
VL - 2020
Y2 - 5 y3 - 22
ID - 8304
ER -
TY - JOUR
AB - The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a
mindful analysis of the possible inflammatory response which could limit their use
in the clinic. Despite the anti-cancer properties of Ag NPs having been widely
demonstrated, there are still few studies concerning their involvement in the
activation of specific inflammatory pathways. The inflammatory outcome depends on
the synthetic route used in the NPs production, in which toxic reagents are
employed. In this work, we compared two types of Ag NPs, obtained by two different
chemical routes: conventional synthesis using sodium citrate and a green protocol
based on leaf extracts as a source of reduction and capping agents. A careful
physicochemical characterization was carried out showing spherical and stable Ag
NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs
respectively. Then, we evaluated their ability to induce the activation of
inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages
using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL
respectively) and two-time points (24 h and 48 h). Our results showed a clear
difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6-8 (IL-6, IL-8)
secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression
exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by
macrophages compared to conventional Ag NPs which induced the activation of all the
factors mentioned above. Subsequently, the exposure of breast cancer cell line
(MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the
conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast
cells) the green Ag NPs did not cause a significant decrease in cell viability.
AN - rayyan-553781789
AU - Cascione, M.
AU - Rizzello, L.
AU - Manno, D.
AU - Serra, A.
AU - De Matteis, V.
DO - 10.3390/ma15030775
IS - 3
J2 - Materials (Basel)
KW - Humanities
Humanism
Humans
Monocytes
Inflammation
LA - eng
N1 - Department of Mathematics and Physics "Ennio De Giorgi", University of
Salento, Via Arnesano, 73100 Lecce, Italy.; Department of Pharmaceutical Sciences
(DISFARM), University of Milan, Via G. Balzaretti 9, 20133 Milan, Italy.; Institute
for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and
Technology, Baldiri Reixac 10-12, 08028 Barcelona, Spain.; National Institute of
Molecular Genetics (INGM), Via F. Sforza 35, 20122 Milan, Italy.; Department of
Mathematics and Physics "Ennio De Giorgi", University of Salento, Via Arnesano,
73100 Lecce, Italy.; Department of Mathematics and Physics "Ennio De Giorgi",
University of Salento, Via Arnesano, 73100 Lecce, Italy.; Department of Mathematics
and Physics "Ennio De Giorgi", University of Salento, Via Arnesano, 73100 Lecce,
Italy.
PY - 2022
SN - 1996-1944 (Print)
ST - Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic
Monocytes
T2 - Materials (Basel, Switzerland)
TI - Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic
Monocytes
VL - 15
Y2 - 1 y3 - 20
ID - 9719
ER -
TY - JOUR
AB - Aims: Capped silver nanoparticles that can be coupled to a variety of
molecules and biomolecules are of great interest owing to their potential
applications in biomedicine. However, there are no data about their toxicity or
functional effects on a key innate immune response, such as IL-6 secretion, after
the engagement of the main group of pathogen-associated molecular patterns
receptors, that is, the Toll-like receptors (TLRs). Materials & methods: N-(2-
mercaptopropionyl)glycine (tiopronin)-capped silver (Agτiopronin) nanoparticles of
a narrow sized distribution (∼5 nm) were synthesized and characterized by
transmission electron microscopy, Fourier transform infrared spectroscopy, Raman,
1H-NMR and total correlation spectroscopy. Cytotoxicity was determined by lactate
deshidrogenase and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium assays in
Raw 264.7 macrophages. IL-6 was measured by ELISA. Results & discussion:
Agτiopronin nanoparticles have a narrow size distribution (≈5 nm), high solubility
and stability in aqueous environment with no cytotoxicity in terms of mitochondrial
function or plasma-membrane integrity at concentrations as high as 200 μg/106
cells. Agτiopronin nanoparticles were not proinflammatory agents, but remarkably
they specifically impaired the IL-6 secretion mediated by TLR2, TLR2/6, TLR3 or
TLR9 stimulation in co-treatment experiments. However, in pretreatment experiments,
nanoparticles enhanced the susceptibility of macrophages to inflammatory
stimulation mediated by TLR2/1 and TLR2/6 specific ligands while severely impairing
the IL-6 secretion activated by the TLR3 or TLR9 ligands. Conclusions: Contrary to
what is found for bare silver nanoparticles, Agτiopronin nanoparticles are
noncytotoxic to macrophages. Agτiopronin nanoparticles showed differential effects
on TLR signaling of a high degree of specificity, without proinflammatory effects
by themselves. These effects have to be borne in mind when using bioconjugates of
Agτiopronin nanoparticles for future medical applications. © 2008 Future Medicine
Ltd.
AN - rayyan-553780273
AU - Castillo, P. M.
AU - Herrera, J. L.
AU - Fernandez-Montesinos, R.
AU - Caro, C.
AU - Zaderenko, A. P.
AU - Mejias, J. A.
AU - Pozo, D.
DO - 10.2217/17435889.3.5.627
IS - 5
KW - Immunoregulation
Innate immunity
Macrophages
Metal nanoparticles
Tiopronin
Toll-like receptors
Toxicity
Animals
Cell Line
Interleukin-6
Nanoparticles
Silver
Spectroscopy, Fourier Transform Infrared
Thiopronine
Toll-Like Receptor 2
Toll-Like Receptors
interleukin 6
metal nanoparticle
nanocoating
silver nanoparticle
tiopronin
toll like receptor
unclassified drug
animal cell
article
controlled study
cytokine release
cytotoxicity
drug coating
drug distribution
drug solubility
drug stability
immunomodulation
inflammation
innate immunity
macrophage
nonhuman
priority journal
proton nuclear magnetic resonance
Raman spectrometry
signal transduction
toxicity testing
PY - 2008
SP - 627-635
ST - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
mediated by Toll-like receptor ligands
T2 - Nanomedicine
TI - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
56549094531&doi=10.2217%2f17435889.3.5.627&partnerID=40&md5=0535acbdaadd8aa6eca147c
04d4270ca
VL - 3
ID - 8305
ER -
TY - JOUR
AB - AIMS: Capped silver nanoparticles that can be coupled to a variety of
molecules and biomolecules are of great interest owing to their potential
applications in biomedicine. However, there are no data about their toxicity or
functional effects on a key innate immune response, such as IL-6 secretion, after
the engagement of the main group of pathogen-associated molecular patterns
receptors, that is, the Toll-like receptors (TLRs). MATERIALS & METHODS: N-(2-
mercaptopropionyl)glycine (tiopronin)-capped silver (Ag@tiopronin) nanoparticles of
a narrow sized distribution ( approximately 5 nm) were synthesized and
characterized by transmission electron microscopy, Fourier transform infrared
spectroscopy, Raman, (1)H-NMR and total correlation spectroscopy. Cytotoxicity was
determined by lactate deshidrogenase and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl
tetrazolium assays in Raw 264.7 macrophages. IL-6 was measured by ELISA. RESULTS &
DISCUSSION: Ag@tiopronin nanoparticles have a narrow size distribution
( approximately 5 nm), high solubility and stability in aqueous environment with no
cytotoxicity in terms of mitochondrial function or plasma-membrane integrity at
concentrations as high as 200 microg/10(6) cells. Ag@tiopronin nanoparticles were
not proinflammatory agents, but remarkably they specifically impaired the IL-6
secretion mediated by TLR2, TLR2/6, TLR3 or TLR9 stimulation in co-treatment
experiments. However, in pretreatment experiments, nanoparticles enhanced the
susceptibility of macrophages to inflammatory stimulation mediated by TLR2/1 and
TLR2/6 specific ligands while severely impairing the IL-6 secretion activated by
the TLR3 or TLR9 ligands. CONCLUSIONS: Contrary to what is found for bare silver
nanoparticles, Ag@tiopronin nanoparticles are noncytotoxic to macrophages.
Ag@tiopronin nanoparticles showed differential effects on TLR signaling of a high
degree of specificity, without proinflammatory effects by themselves. These effects
have to be borne in mind when using bioconjugates of Ag@tiopronin nanoparticles for
future medical applications.
AN - rayyan-553782102
AU - Castillo, P. M.
AU - Herrera, J. L.
AU - Fernandez-Montesinos, R.
AU - Caro, C.
AU - Zaderenko, A. P.
AU - Mejías, J. A.
AU - Pozo, D.
DO - 10.2217/17435889.3.5.627
IS - 5
J2 - Nanomedicine (Lond)
KW - Animals
Cell Line
Interleukin-6/*metabolism
Macrophages/cytology/*drug effects/metabolism
Nanoparticles/chemistry/ultrastructure
Silver/chemistry/*pharmacology
Tiopronin/*pharmacology
Toll-Like Receptor 2/metabolism
Toll-Like Receptors/*metabolism
Toll-Like Receptors
Tiopronin
LA - eng
N1 - Department of Physical, Chemical & Natural Systems, Pablo de Olavide
University, Seville, Spain.
PY - 2008
SP - 627-35
ST - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
T2 - Nanomedicine (London, England)
TI - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
VL - 3
Y2 - 10
ID - 10013
ER -
TY - JOUR
AB - Antibodies to Circumsporozoite protein (CSP) confer protection against
controlled human malaria infection (CHMI) caused by the parasite Plasmodium
falciparum. Although CSP is highly immunogenic, it does not induce long lasting
protection and efforts to improve CSP-specific immunological memory and duration of
protection are underway. We have previously reported that the clinical grade CSP
vaccine FMP013 was immunogenic and protective against malaria challenge in mice
when combined with the Army Liposomal Formulation adjuvant containing immune
modulators 3D-PHAD (TM) and QS21 (ALFQ). To move forward with clinical evaluation,
we now report the safety, toxicity and immunogenicity of clinical grade FMP013 and
ALFQ in Rhesus macaques. Three groups of Rhesus (n = 6) received half or full human
dose of FMP013 + ALFQ on a 0-1-2 month schedule, which showed mild local site
reactions with no hematologic derangements in red blood cell homeostasis, liver
function or kidney function. Immunization induced a transient systemic inflammatory
response, including elevated white blood cell counts, mild fever, and a few
incidences of elevated creatine kinase, receding to normal range by day 7 post
vaccination. Optimal immunogenicity in Rhesus was observed using a 1 mL ALFQ+ 20 mu
g FMP013 dose. Doubling the FMP013 antigen dose to 40 mu g had no effect while
halving the ALFQ adjuvant dose to 0.5 mL lowered immunogenicity. Similar to data
generated in mice, FMP013 + ALFQ induced serum antibodies that reacted to all
regions of the CSP molecule and a Th1-biased cytokine response in Rhesus. Rhesus
antibody response to FMP013 + ALFQ was found to be non-inferior to historical
benchmarks including that of RTS,S + AS01 in humans. A four-dose GLP toxicity study
in rabbits confirmed no local site reactions and transient systemic inflammation
associated with ALFQ adjuvant administration. These safety and immunogenicity data
support the clinical progression and testing of FMP013 + ALFQ in a CHMI trial in
the near future. (C) 2019 The Author(s). Published by Elsevier Ltd.
AN - rayyan-553780277
AU - Cawlfield, A.
AU - Genito, C. J.
AU - Beck, Z.
AU - Bergmann-Leitner, E. S.
AU - Bitzer, A. A.
AU - Soto, K.
AU - Zou, X. Y.
AU - Hadiwidjojo, S. H.
AU - Gerbasi, R. V.
AU - Mullins, A. B.
AU - Noe, A.
AU - Waters, N. C.
AU - Alving, C. R.
AU - Matyas, G. R.
AU - Dutta, S.
DO - 10.1016/j.vaccine.2019.05.059
IS - 29
KW - Liposomes
Vaccination
PY - 2019
SN - 0264-410X 1873-2518
SP - 3793-3803
ST - Safety, toxicity and immunogenicity of a malaria vaccine based on the
circumsporozoite protein (FMP013) with the adjuvant army liposome formulation
containing QS21 (ALFQ)
T2 - VACCINE
TI - Safety, toxicity and immunogenicity of a malaria vaccine based on the
circumsporozoite protein (FMP013) with the adjuvant army liposome formulation
containing QS21 (ALFQ)
VL - 37
Y2 - 6 y3 - 27
ID - 8309
ER -
TY - JOUR
AB - Halloysite is a naturally-occurring nanomaterial occurring in the thousands
of tons and that serves as biomaterial, with applications in the areas of
biotechnology, pharmaceutical, and medical research. This study reports on the
anti-inflammatory, cytotoxic, and anti-oxidant activity of halloysite Jarrahdale
(collected at ~45km SE of Perth, Western Australia; JA), Dragon Mine (provided by
Natural Nano Inc., Rochester, New York; NA), and Kalgoorie Archean (collected at
Siberia, ~85km NW of Kalgoorlie, West Australia; PA). Prior to biological testing,
halloysites were characterized by 27Al and 29Si Nuclear Magnetic Resonance
Spectroscopy, the anti-inflammatory activity was determined by (a) the mouse ear
edema method, using 12-o-tetradecanoylphorbol-13-acetate (TPA) as anti-inflammatory
agent; and (b) the myeloperoxidase enzymatic activity method (MPO). Cell viability
was determined using the MTT method. Sample characterization by NMR method showed
similar symmetry and atomic environments, with no evidence of distortion(s) due to
shiftings in atomic ordering or electron density. The anti-inflammatory activity
followed the order: PA>JA>NA, and remained invariant with time. Prolonged anti-
inflammatory activity related inversely to surface area and lumen space. The low
extent of infiltration at shorter reaction times confirmed a limiting number of
active surface sites. EPR intensity signals followed the order: JA>NA>PA. The poor
stabilization of RO species in PA suspensions was explained by tube alignment
provoking occlusion, thus limiting transfer of H+ or e- from-and-to the surface,
and decreases in acidity associated to Aloct. Cell viability (%) varied from one
surface to the other, PA(92.3±6.0), JA(84.9±7.8), and NA(78.0±5.6), but related
directly to SBET values. © 2013 Elsevier B.V.
AN - rayyan-553780280
AU - Cervini-Silva, J.
AU - Nieto-Camacho, A.
AU - Palacios, E.
AU - Montoya, J. A.
AU - Gómez-Vidales, V.
AU - Ramírez-Apán, M. T.
DO - 10.1016/j.colsurfb.2013.06.056
KW - Surface morphology
Time-dependent infiltration
Aluminum Silicates
Animals
Cell Death
Cell Survival
Electrons
Ion Exchange
Macrophages, Peritoneal
Magnetic Resonance Spectroscopy
Mice
Peroxidase
Surface Plasmon Resonance
Surface Properties
Tetradecanoylphorbol Acetate
Biological materials
Cytotoxicity
Nuclear magnetic resonance spectroscopy
aluminum
dragon mine
hallosite
hydrogen
indometacin
jarrahdale
kalgoorie archean
myeloperoxidase
nanomaterial
phorbol 13 acetate 12 myristate
silver
unclassified drug
aluminum silicate
antiinfective agent
clay
peroxidase
Active surface sites
Anti-inflammatories
Anti-inflammatory agents
Enzymatic activities
Perth , Western Australia
analytic method
animal cell
animal experiment
animal model
article
cell infiltration
cell viability
density
drug cytotoxicity
drug determination
ear edema
electron spin resonance
enzyme activity
mouse
nonhuman
priority journal
protein stability
proton transport
reaction time
surface property
suspension
animal
cell death
cell survival
cytology
drug effects
electron
ion exchange
metabolism
peritoneum macrophage
Kaolinite
PY - 2013
SP - 651-655
ST - Anti-inflammatory and anti-bacterial activity, and CYTOTOXICITY of halloysite
surfaces
T2 - Colloids and Surfaces B: Biointerfaces
TI - Anti-inflammatory and anti-bacterial activity, and CYTOTOXICITY of halloysite
surfaces
84881245846&doi=10.1016%2fj.colsurfb.2013.06.056&partnerID=40&md5=2eb374e95d08c917a
280dc3d75618485
VL - 111
ID - 8312
ER -
TY - JOUR
AB - SUMMARY: The aim of this study is to determine the potential therapeutic
effects of CAPE in CP-induced nephrotoxicity in rats. Cisplatin (CP) is an
antineoplastic chemotherapeutic used for treatment of many cancer types but its
applications may induce nephrotoxicity. Caffeic acid phenethyl ester (CAPE) is an
active component of propolis and it has several important physiological activities.
Rats were divided into four groups: Control, CAPE (10 µmol/kg/i.p), CP (7
mg/kg/i.p), and CP+CAPE (7 mg/kg/i.p, CP and 10 µmol/kg/i.p, CAPE). After
administrations, animals were sacrificed, and kidney tissues were extracted.
Histopathological changes were evaluated and TNF-α and IL-6 immunostaining were
performed. Moreover, tissue SOD, CAT and MDA levels were measured by ELISA assay to
assessment of oxidative stress and lipid peroxidation. CP group showed
histopathological deterioration compared to the Control group and CAPE treatment
attenuated this damage. When compared with Control and CAPE group, an increase in
TNF-α and IL-6 immunoreactivities and tissue MDA levels were observed in the CP
group while a decrease in tissue SOD and CAT levels were detected. Furthermore, an
improvement was observed in the CP+CAPE compared to the CP group. We suggest that
CAPE can be used as a therapeutic agent to attenuate the toxic effects of
cisplatin, thanks to its antioxidant and anti-inflammatory properties. RESUMEN: El
objetivo de este estudio fue determinar los posibles efectos terapéuticos de éster
fenetílico del ácido cafeico (EFAC) en la nefrotoxicidad inducida por cisplatino
(CP) en ratas. El CP es un quimioterapéutico antineoplásico utilizado para el
tratamiento de muchos tipos de cáncer, sin embargo sus aplicaciones pueden inducir
nefrotoxicidad. El EFAC es un componente activo del propóleo y tiene varias
actividades fisiológicas importantes. Para el estudio las ratas se dividieron en
cuatro grupos: Control, EFAC (10 µmol / kg / ip), CP (7 mg / kg / ip) y CP + EFAC
(7 mg / kg / ip, CP y 10 µmol / kg / ip, EFAC). Después de las administraciones, se
sacrificaron los animales y se extrajeron los tejidos renales. Se evaluaron los
cambios histopatológicos y se realizó inmunotinción de TNF-α e IL-6. Además, los
niveles tisulares de SOD, CAT y MDA se midieron mediante un ensayo ELISA para
evaluar el estrés oxidativo y la peroxidación lipídica. El grupo CP mostró
deterioro histopatológico en comparación con el grupo Control y el tratamiento con
EFAC atenuó este daño. En comparación con el grupo de control y EFAC, se observó un
aumento en las inmunorreactividades de TNF-α e IL-6 y los niveles de MDA en el
tejido en el grupo de CP, mientras que se detectó una disminución en los niveles de
SOD y CAT en los tejidos. Además, se observó una mejora en el CP + EFAC en
comparación con el grupo CP. Sugerimos que EFAC puede utilizarse como agente
terapéutico para atenuar los efectos tóxicos del cisplatino, gracias a sus
propiedades antioxidantes y antiinflamatorias.
AN - rayyan-553780281
AU - Ceylan, Tayfun
AU - Kaymak, Emin
AU - Akin, Ali Tugrul
AU - Yakan, Birkan
DO - 10.4067/S0717-95022021000200612
IS - 2
KW - Caffeic acid phenethyl ester
Cisplatin
Cisplatino
Estrés oxidativo
Inflamación
Inflammation
Oxidative stress
Éster fenetílico del ácido cafeico
Oxidative Stress
LA - en
PY - 2021
SN - 0717-9367
SP - 612-618
ST - The ameliorative effects of caffeic acid phenethyl ester in cisplatin-induced
nephrotoxicity: assessment of the oxidative stress an inflammation
TI - The ameliorative effects of caffeic acid phenethyl ester in cisplatin-induced
nephrotoxicity: assessment of the oxidative stress an inflammation
VL - 39
Y2 - 4 y3 - 1
ID - 8313
ER -
TY - JOUR
AB - The increased use of nano-sized metallic materials is likely to result in the
release of these particles into the environment. It is, however, unclear if these
materials are harmful to aquatic animals. Furthermore, because the dissolution of
such nanomaterials will occur, it is probable that some of the adverse effects
resulting will result from the dissolved metal species. In this study, therefore,
we investigated the health and environmental impact of silver nanoparticles (Ag-
NPs) on Japanese Medaka by studying changes in the expression of stress-related
genes using real time RT-PCR analysis and compared these results with those of
Medaka exposed to soluble silver ions. The stress-related genes selected here were
metallothionein, HSP 70, GST, p53, CYP 1A and the transferrin gene. The expression
levels of each gene were determined using two different Ag-NPs dosages and were
quantified by measuring the mRNA concentrations in liver extracts with the Taqman-
based Real-Time PCR method. The results suggest that these two silver forms have
distinguishable toxic fingerprints between them. While the Ag-NPs led to cellular
and DNA damage, as well as carcinogenic and oxidative stresses, genes related with
metal detoxification/metabolism regulation and radical scavenging action were also
induced. In contrast, the ionic silver led to an induction of inflammatory response
and metallic detoxification processes in the liver of the exposed fish, but
resulted in a lower overall stress response when compared with the Ag-NPs. © 2009
Elsevier B.V. All rights reserved.
AN - rayyan-553780282
AU - Chae, Y. J.
AU - Pham, C. H.
AU - Lee, J.
AU - Bae, E.
AU - Yi, J.
AU - Gu, M. B.
DO - 10.1016/j.aquatox.2009.07.019
IS - 4
KW - Japanese Medaka
Nanotoxicity
Real-time PCR
Silver nanoparticle
Animals
DNA Damage
Liver
Metal Nanoparticles
Oryzias
Oxidative Stress
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Silver
Water Pollutants, Chemical
Animalia
Oryzias latipes
Oryziinae
cytochrome P450 1A
messenger RNA
metallothionein
nanoparticle
protein p53
silver
silver nanoparticle
transferrin
unclassified drug
concentration (composition)
detoxification
dissolution
dose-response relationship
environmental impact
fish
mitochondrial DNA
toxicity test
animal experiment
animal tissue
article
carcinogenesis
cell damage
controlled study
DNA damage
ecotoxicology
environmental impact assessment
inflammation
liver parenchyma
metal metabolism
nanotoxicology
nonhuman
nucleotide sequence
oxidative stress
priority journal
stress
water contamination
PY - 2009
SP - 320-327
ST - Evaluation of the toxic impact of silver nanoparticles on Japanese medaka
(Oryzias latipes)
T2 - Aquatic Toxicology
TI - Evaluation of the toxic impact of silver nanoparticles on Japanese medaka
(Oryzias latipes)
69949141979&doi=10.1016%2fj.aquatox.2009.07.019&partnerID=40&md5=bd40c9314b5b3ce4e2
abfc04c1a21ced
VL - 94
ID - 8314
ER -
TY - JOUR
AB - In the current study, silver nanoparticles (AgNPs) were biosynthesized using
galbanic acid (GA), a sesquiterpene coumarin. The formation of GA-AgNPs was
characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform
infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), Energy
Dispersive X-ray (EDX), and X-ray diffraction (XRD). The biosynthesized GA-AgNPs
were spherical in shape with an average particle size of 142.33 +/- 32.6 nm. The
results from the antibacterial assays sug-gested that biosynthesized GA-AgNPs were
more potent against multi-drug resistant (MDR) and non -MDR pathogenic bacteria
than the crude GA alone. The nanoparticles also showed potent antiproliferative
potential against H1229 and MCF-7 cancer cells with IC50 values of 25 mu g/mL and
50 mu g/mL by the MTT assay, respectively. These NPs showed good antioxidant (30 %
at 100 mu g/mL), anti-inflammatory (99.5 % at 500 mu g/mL), and anti-coagulant
properties without significant hemolysis on red blood cells (RBCs). These results
confirm the benefits of using the green, simple, and cost-effective manner for the
synthesis of AgNPs with excellent biological properties and hemocompatibility.(c)
2022 The Society of Powder Technology Japan. Published by Elsevier B.V. and The
Society of Powder Technology Japan. All rights reserved.
AN - rayyan-553780283
AU - Chahardoli, A.
AU - Mavaei, M.
AU - Shokoohinia, Y.
AU - Fattahi, A.
DO - 10.1016/j.apt.2022.103928
IS - 1
KW - Sesquiterpenes
Antioxidants
PY - 2023
SN - 0921-8831 1568-5527
ST - Galbanic acid, a sesquiterpene coumarin as a novel candidate for the
biosynthesis of silver nanoparticles: In vitro hemocompatibility,
antiproliferative, antibacterial, antioxidant, and anti-inflammatory properties
T2 - ADVANCED POWDER TECHNOLOGY
TI - Galbanic acid, a sesquiterpene coumarin as a novel candidate for the
biosynthesis of silver nanoparticles: In vitro hemocompatibility,
antiproliferative, antibacterial, antioxidant, and anti-inflammatory properties
VL - 34
Y2 - 1
ID - 8315
ER -
TY - JOUR
AB - Serum amyloid A3 (Saa3) derives mainly from extrahepatic tissue and is not
detected in plasma from moderately inflamed obese mice. In contrast, it is present
in plasma from mice acutely inflamed by injection of high dose of
lipopolysaccharide (LPS). To reconcile these differences, we evaluated whether
different acute inflammatory stimuli could affect the presence of Saa3 in plasma.
Saa3 appeared dose dependently in plasma after LPS injection. In contrast, only
very low levels were detected after sterile inflammation with silver nitrate
despite levels of Saa1 and Saa2 being comparable to high dose LPS. Saa3 was not
detected in plasma following casein administration. Although most Saa3 was found in
HDL, a small amount was not lipoprotein associated. Gene expression and proteomic
analysis of liver and adipose tissue suggested that a major source of Saa3 in
plasma after injection of LPS was adipose tissue rather than liver. We conclude
that Saa3 only appears in plasma after induction of acute inflammation by some but
not all inflammatory stimuli. These findings are consistent with the observation
that Saa3 is not detectable in plasma in more moderate chronic inflammatory states
such as obesity.
AN - rayyan-553782185
AU - Chait, A.
AU - den Hartigh, L. J.
AU - Wang, S.
AU - Goodspeed, L.
AU - Babenko, I.
AU - Altemeier, W. A.
AU - Vaisar, T.
DO - 10.1038/s41598-020-66898-7
IS - 1
J2 - Sci Rep
KW - Adipose Tissue/*metabolism
Animals
Anti-Infective Agents, Local/pharmacology/toxicity
*Gene Expression Regulation
Inflammation/blood/chemically induced/*pathology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Serum Amyloid A Protein/*physiology
Silver Nitrate/*toxicity
Serum Amyloid A Protein
LA - eng
N1 - Divisions of Metabolism, Endocrinology and Nutrition, University of
Washington, Seattle, WA, 98109, USA. achait@uw.edu.; Divisions of Metabolism,
Endocrinology and Nutrition, University of Washington, Seattle, WA, 98109, USA.;
Divisions of Metabolism, Endocrinology and Nutrition, University of Washington,
Seattle, WA, 98109, USA.; Divisions of Metabolism, Endocrinology and Nutrition,
University of Washington, Seattle, WA, 98109, USA.; Divisions of Metabolism,
Endocrinology and Nutrition, University of Washington, Seattle, WA, 98109, USA.;
Pulmonary, Critical Care and Sleep Medicine, University of Washington, Seattle, WA,
98109, USA.; Divisions of Metabolism, Endocrinology and Nutrition, University of
Washington, Seattle, WA, 98109, USA.
PY - 2020
SP - 10397
ST - Presence of serum amyloid A3 in mouse plasma is dependent on the nature and
extent of the inflammatory stimulus
T2 - Scientific reports
TI - Presence of serum amyloid A3 in mouse plasma is dependent on the nature and
extent of the inflammatory stimulus
VL - 10
Y2 - 6 y3 - 25
ID - 10095
ER -
TY - JOUR
AB - Since the discovery of mutant Janus Kinase 2 (JAK2), JAK2V617F, in a major
proportion of myeloproliferative neoplasm (MPN) patients, there has been a flurry
of activity in the development of JAK2 inhibitors. Pan-JAK, predominantly JAK2 and
off-target JAK2 inhibitors have been developed in the short span of the past 5
years. These compounds have since been tested to varying success in both in vitro
and in vivo settings with several proceeding on to advanced clinical trials.
Although it was hoped that these inhibitors would be the silver bullet in the
manner than imatinib was to chronic myeloid leukemia, it is becoming apparent that
this is not the case for various reasons, chief of which is that a significant
reduction of the underlying pathogenic clone is not achieved. In fact, the very
notion that the target of JAK2 inhibitors (be it pan-JAK or JAK2 specific) is the
mutant JAK2V617F is being challenged with findings from several clinical trials
showing a poor correlation between the reduction in JAK2V617F mutant allele burden
and clinical response. In view of this, it is not surprising that several groups
are now investigating combinations of JAK2 inhibitors and other agents in MPN.
Although much knowledge has been added in this short span of time, it is apparent
that our understanding of the role of JAK2 inhibitors in the treatment scheme of
MPN is only beginning. © 2011, SAGE Publications. All rights reserved.
AN - rayyan-553780285
AU - Chan, D.
AU - Koren Michowitz, M.
DO - 10.1177/2040620711401646
IS - 2
KW - JAK2 inhibitors
JAK2V617F
myeloproliferative neoplasm
1 cyclopropyl 3 [3 (5 morpholinomethyl 1h benzimidazol 2 yl) 1h pyrazol 4 yl]urea
5 chloro n2 [1 (5 fluoro 2 pyrimidinyl)ethyl] n4 (5 methyl 1h pyrazol 3 yl) 2,4
pyrimidinediamine
anagrelide
antithrombocytic agent
bms 911543
C reactive protein
erlotinib
gandotinib
givinostat
hydroxyurea
interleukin 1 receptor blocking agent
interleukin 6
Janus kinase 2 inhibitor
Janus kinase inhibitor
lenalidomide
lestaurtinib
ls 104
macrophage inflammatory protein 1beta
momelotinib
n tert butyl 3 [5 methyl 2 [4 [2 (1 pyrrolidinyl)ethoxy]phenylamino] 4
pyrimidinylamino]benzenesulfonamide
ns 018
ns 108
pacritinib
panobinostat
peginterferon
pomalidomide
ruxolitinib
tofacitinib
unclassified drug
wp 1066
xl 019
abdominal pain
acute leukemia
advanced cancer
allele
anemia
blast transformation
bone marrow suppression
cancer chemotherapy
chronic myelomonocytic leukemia
cytoreductive surgery
diarrhea
drug activity
drug cytotoxicity
drug dose reduction
drug efficacy
drug response
drug tolerability
fatigue
follow up
gene mutation
hematologic disease
Hodgkin disease
human
leukocytosis
lung metastasis
lung non small cell cancer
lymphoma
myelofibrosis
myeloid leukemia
myeloid metaplasia
myelomonocytic leukemia
nausea
neurotoxicity
neutropenia
nonhuman
pathogenesis
phase 2 clinical trial (topic)
phlebotomy
polycythemia vera
postoperative complication
priority journal
protein blood level
rash
review
splenomegaly
symptom assessment
thrombocythemia
thrombocytopenia
thrombocytosis
PY - 2011
SP - 61-71
ST - Update on JAK2 inhibitors in myeloproliferative neoplasm
T2 - Therapeutic Advances in Hematology
TI - Update on JAK2 inhibitors in myeloproliferative neoplasm
84993810398&doi=10.1177%2f2040620711401646&partnerID=40&md5=8bc6533748879e0b12a1660
13d43339e
VL - 2
ID - 8317
ER -
TY - JOUR
AB - In this report, a novel nanocomposite based on type I collagen (Col) was
prepared, which contained varying amounts (15.1, 30.2, and 75.5 ppm) of silver
nanoparticles (AgNPs). The surface morphology and chemical composition pure Col and
Col-AgNP nanocomposites (Col-Ag) was characterized by UV–Vis spectroscopy (UV–Vis),
atomic force microscope (AFM) and Fourier transform IR spectrometer (FTIR). The
biocompatibility effect and biological activity of Col-Ag culturing with
mesenchymal stem cells (MSCs), as well as guiding for angiogenesis differentiation,
were evaluated via in vitro assay. The Col-Ag in 30.2 ppm demonstrated better
biological properties and compatibility culturing with MSCs. The biological
properties could be associated with cell adhesion, proliferation, migration and
differentiation. Afterwards, the induced angiogenesis and differentiation of MSCs
by the expression of von Willebrand Factor (vWF) and CD31 were also investigated.
Furthermore, both anti-inflammatory and endothelialization ability were also
investigated in vivo assay. It was observed that Col-Ag nanocomposites not only
inhibited CD86 expression, but also facilitated endothelialization capacity, the
expression of CD31 when implanting Col-Ag into rats subcutaneously after 4 weeks.
This current research indicates that Col-Ag nanocomposites has potential of being
employed as a surface modification approach, and is better in clinical treatments
with MSCs for vascular regeneration applications. © 2021
AN - rayyan-553780286
AU - Chang, K. B.
AU - Shen, C. C.
AU - Hsu, S. H.
AU - Tang, C. M.
AU - Yang, Y. C.
AU - Liu, S. Y.
AU - Ku, T. R.
AU - Kung, M. L.
AU - Hsieh, H. H.
AU - Hung, H. S.
DO - 10.1016/j.colsurfa.2021.126814
Differentiation
Mesenchymal stem cells
Type I collagen
Vascular regeneration
Atomic force microscopy
Cell culture
Clinical research
Collagen
Differentiation (calculus)
Morphology
Nanocomposites
Spectrometers
Stem cells
Surface morphology
actin
collagen type 1
Hermes antigen
integrin
nanocomposite
platelet endothelial cell adhesion molecule 1
silver nanoparticle
stromal cell derived factor 1alpha
vasculotropin
vasculotropin receptor 2
von Willebrand factor
Ag$++$
Angiogenesis
Biological properties
Endothelialization
Functionalized
Mesenchymal stem cell
Type-I collagen
angiogenesis
animal cell
animal experiment
apoptosis
Article
biocompatibility
cell adhesion
cell interaction
cell migration
cell proliferation
cell survival rate
cell viability
chemical structure
collagen fiber
comparative study
controlled study
cytoskeleton
female
flow cytometry
fluorescence activated cell sorting
Fourier transform spectroscopy
human
human cell
immunophenotyping
in vitro study
in vivo study
nonhuman
protein expression
rat
stem cell culture
surface property
tissue regeneration
TUNEL assay
zeta potential
PY - 2021
ST - Functionalized collagen-silver nanocomposites for evaluation of the
biocompatibility and vascular differentiation capacity of mesenchymal stem cells
T2 - Colloids and Surfaces A: Physicochemical and Engineering Aspects
TI - Functionalized collagen-silver nanocomposites for evaluation of the
biocompatibility and vascular differentiation capacity of mesenchymal stem cells
85106900438&doi=10.1016%2fj.colsurfa.2021.126814&partnerID=40&md5=66dfcd08c6e7afb9c
2af601d9c8b37eb
VL - 624
ID - 8318
ER -
TY - JOUR
AB - With the development of nanotechnology, metal-containing nanoparticles are
used widely in the diagnosis, monitoring and treatment of central nervous system
(CNS) diseases. The neurotoxicity of these nanoparticles has drawn attention. Glial
cells (particularly microglial cells and astrocytes) have important functions in
the CNS. Neural disorders are related to functional/histologic damage to glial
cells. Dysfunctions of microglial cells or astrocytes injure the brain, and cause
the neurodegeneration seen in Alzheimer's disease and Parkinson's disease. We have
summarized the route of access of metal-containing nanoparticles to the CNS, as
well as their neurotoxicity and potential molecular mechanisms involved in glial
cells. Metal-containing nanoparticles cross or bypass the blood-brain barrier,
access the CNS and cause neurotoxicity. The potential mechanisms are related to
inflammation, oxidative stress, DNA and/or mitochondrial damage and cell death, all
of which are mediated by microglial cell activation, inflammatory factor release,
generation of reactive oxygen species, apoptosis and/or autophagy in glial cells.
Moreover, these processes increase the burden of the CNS and even accelerate the
occurrence or development of neurodegenerative diseases. Some important signaling
pathways involved in the mechanism of neurotoxicity in glial cells caused by
nanoparticles are also discussed.
AN - rayyan-553780287
AU - Chang, X. R.
AU - Li, J. Y.
AU - Niu, S. Y.
AU - Xue, Y. Y.
AU - Tang, M.
DO - 10.1002/jat.4037
IS - 1
PY - 2021
SN - 0260-437X 1099-1263
SP - 65-81
ST - Neurotoxicity of metal-containing nanoparticles and implications in glial
cells
TI - Neurotoxicity of metal-containing nanoparticles and implications in glial
cells
VL - 41
Y2 - 1
ID - 8319
ER -
TY - JOUR
AB - The aim of this work is to understand the potential health effects of metal
nanoparticles by exposing human leukemic cell lines (jurkat, K562 and KG1A cells)
to nanosize phosphonomethyl iminodiacetic acid coated cobalt oxide (PMIDA-CoO) NPs.
The synthesized PMIDA-CoO NPs were characterized by XRD, dynamic light scattering,
transmission electron microscopy and scanning electron microscopy. Our results
showed that exposure of leukemic cell lines to PMIDA-CoO NPs caused reactive oxygen
species (ROS) generation by increasing the concentration of free Co++ ions in
cancer microenvironment. But at physiological pH, PMIDA-CoO liberates little amount
of Co++ ions into media and exerts lower toxicity to normal cells up to a certain
dose. PMIDA-CoO NPs caused DNA damage in leukemic cell lines, which was reflected
by an increase in apoptosis of jurkat, KG-1A and K562 cells. PMIDA-CoO NPs induced
apoptosis by increasing pro-inflammatory cytokines, primarily TNF-alpha. The in
vivo study shows that PMIDA-CoO NPs were efficiently killed DLA cells. These
findings have important implications for understanding the potential anticancer
property induced by surface-modified cobalt oxide nanoparticles.
AN - rayyan-553780288
AU - Chattopadhyay, S.
AU - Dash, S. K.
AU - Tripathy, S.
AU - Pramanik, P.
AU - Roy, S.
DO - 10.1007/s00775-014-1221-7
IS - 1
KW - Humans
Humanities
Humanism
PY - 2015
SN - 0949-8257 1432-1327
SP - 123-141
ST - Phosphonomethyl iminodiacetic acid-conjugated cobalt oxide nanoparticles
liberate Co++ ion-induced stress associated activation of TNF-alpha/p38
MAPK/caspase 8-caspase 3 signaling in human leukemia cells
T2 - JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
TI - Phosphonomethyl iminodiacetic acid-conjugated cobalt oxide nanoparticles
liberate Co++ ion-induced stress associated activation of TNF-alpha/p38
MAPK/caspase 8-caspase 3 signaling in human leukemia cells
VL - 20
Y2 - 1
ID - 8320
ER -
TY - JOUR
AB - Aloe vera-based hydrogels have emerged as promising platforms for the
delivery of therapeutic agents in wound dressings due to their biocompatibility and
unique wound-healing properties. The present study provides a comprehensive
overview of recent advances in the application of Aloe vera-based hydrogels for
wound healing. The synthesis methods, structural characteristics, and properties of
Aloe vera-based hydrogels are discussed. Mechanisms of therapeutic agents released
from Aloe vera-based hydrogels, including diffusion, swelling, and degradation, are
also analyzed. In addition, the therapeutic effects of Aloe vera-based hydrogels on
wound healing, as well as the reduction of inflammation, antimicrobial activity,
and tissue regeneration, are highlighted. The incorporation of various therapeutic
agents, such as antimicrobial and anti-inflammatory ones, into Aloe vera-based
hydrogels is reviewed in detail. Furthermore, challenges and future prospects of
Aloe vera-based hydrogels for wound dressing applications are considered. This
review provides valuable information on the current status of Aloe vera-based
hydrogels for the delivery of therapeutic agents in wound dressings and highlights
their potential to improve wound healing outcomes.
AN - rayyan-553780290
AU - Chelu, M.
AU - Musuc, A. M.
AU - Popa, M.
AU - Moreno, J. C.
DO - 10.3390/gels9070539
IS - 7
KW - Wound Healing
PY - 2023
SN - 2310-2861
ST - Aloe vera-Based Hydrogels for Wound Healing: Properties and Therapeutic
Effects
T2 - GELS
TI - Aloe vera-Based Hydrogels for Wound Healing: Properties and Therapeutic
Effects
VL - 9
Y2 - 7
ID - 8322
ER -
TY - JOUR
AB - Poor wound healing after diabetes or extensive burn remains a challenging
problem. Recently, we presented a physical approach to fabricate ultrasmall silver
particles from angstrom ngstrom scale to nanoscale and determined the antitumor
efficacy of angstrom ngstrom-scale silver particles (Ag angstrom Ps) in the
smallest size range. Here we used the medium-sized Ag angstrom Ps (65.9 +/- 31.6
angstrom) to prepare carbomer gel incorporated with these larger Ag angstrom Ps (L-
Ag angstrom Ps-gel) and demonstrated the potent broad-spectrum antibacterial
activity of L-Ag angstrom Ps-gel without obvious toxicity on wound healing-related
cells. Induction of reactive oxygen species contributed to L-Ag angstrom Ps-gel-
induced bacterial death. Topical application of L-Ag angstrom Ps-gel to mouse skin
triggered much stronger effects than the commercial silver nanoparticles (AgNPs)-
gel to prevent bacterial colonization, reduce inflammation, and accelerate diabetic
and burn wound healing. L-Ag angstrom Ps were distributed locally in skin without
inducing systemic toxicities. This study suggests that L-Ag angstrom Ps-gel
represents an effective and safe antibacterial and anti-inflammatory material for
wound therapy.
AN - rayyan-553780294
AU - Chen, C. Y.
AU - Yin, H.
AU - Chen, X.
AU - Chen, T. H.
AU - Liu, H. M.
AU - Rao, S. S.
AU - Tan, Y. J.
AU - Qian, Y. X.
AU - Liu, Y. W.
AU - Hu, X. K.
AU - Luo, M. J.
AU - Wang, Z. X.
AU - Liu, Z. Z.
AU - Cao, J.
AU - He, Z. H.
AU - Wu, B.
AU - Yue, T.
AU - Wang, Y. Y.
AU - Xia, K.
AU - Luo, Z. W.
AU - Wang, Y.
AU - Situ, W. Y.
AU - Liu, W. E.
AU - Tang, S. Y.
AU - Xie, H.
DO - 10.1126/sciadv.aba0942
IS - 43
KW - Wound Healing
Inflammation
Gels
PY - 2020
SN - 2375-2548
ST - Angstrom-scale silver particle-embedded carbomer gel promotes wound healing
by inhibiting bacterial colonization and inflammation
T2 - SCIENCE ADVANCES
TI - Angstrom-scale silver particle-embedded carbomer gel promotes wound healing
VL - 6
Y2 - 10
ID - 8326
ER -
TY - JOUR
AB - Poor wound healing after diabetes or extensive burn remains a challenging
problem. Recently, we presented a physical approach to fabricate ultrasmall silver
particles from Ångstrom scale to nanoscale and determined the antitumor efficacy of
Ångstrom-scale silver particles (AgÅPs) in the smallest size range. Here we used
the medium-sized AgÅPs (65.9 ± 31.6 Å) to prepare carbomer gel incorporated with
these larger AgÅPs (L-AgÅPs-gel) and demonstrated the potent broad-spectrum
antibacterial activity of L-AgÅPs-gel without obvious toxicity on wound healing-
related cells. Induction of reactive oxygen species contributed to L-AgÅPs-gel-
induced bacterial death. Topical application of L-AgÅPs-gel to mouse skin triggered
much stronger effects than the commercial silver nanoparticles (AgNPs)-gel to
prevent bacterial colonization, reduce inflammation, and accelerate diabetic and
burn wound healing. L-AgÅPs were distributed locally in skin without inducing
systemic toxicities. This study suggests that L-AgÅPs-gel represents an effective
and safe antibacterial and anti-inflammatory material for wound therapy.
AN - rayyan-553782103
AU - Chen, C. Y.
AU - Yin, H.
AU - Chen, X.
AU - Chen, T. H.
AU - Liu, H. M.
AU - Rao, S. S.
AU - Tan, Y. J.
AU - Qian, Y. X.
AU - Liu, Y. W.
AU - Hu, X. K.
AU - Luo, M. J.
AU - Wang, Z. X.
AU - Liu, Z. Z.
AU - Cao, J.
AU - He, Z. H.
AU - Wu, B.
AU - Yue, T.
AU - Wang, Y. Y.
AU - Xia, K.
AU - Luo, Z. W.
AU - Wang, Y.
AU - Situ, W. Y.
AU - Liu, W. E.
AU - Tang, S. Y.
AU - Xie, H.
DO - 10.1126/sciadv.aba0942
IS - 43
J2 - Sci Adv
KW - Acrylic Resins
Animals
Anti-Bacterial Agents/pharmacology
*Burns/drug therapy
Inflammation/drug therapy
*Metal Nanoparticles
Mice
Silver/pharmacology
Wound Healing
Inflammation
Gels
LA - eng
N1 - Department of Orthopedics, Movement System Injury and Repair Research Center,
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.;
Angmedicine Research Center of Central South University, Changsha, Hunan 410008,
China.; Xiangya Hospital of Central South University-Amcan Pharmaceutical
Biotechnology Co. Ltd. Joint Research Center, Changsha, Hunan 410008, China.;
Department of Orthopedics, Movement System Injury and Repair Research Center,
Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.;
Department of Clinical Laboratory, Xiangya Hospital, Central South University,
Changsha, Hunan 410008, China.; Department of Orthopedics, Movement System Injury
and Repair Research Center, Xiangya Hospital, Central South University, Changsha,
Hunan 410008, China.; Department of Orthopedics, Movement System Injury and Repair
Research Center, Xiangya Hospital, Central South University, Changsha, Hunan
410008, China.; Department of Orthopedics, Movement System Injury and Repair
410008, China.; Xiangya School of Nursing, Central South University, Changsha,
410008, China.; Angmedicine Research Center of Central South University, Changsha,
Hunan 410008, China.; Xiangya Hospital of Central South University-Amcan
Pharmaceutical Biotechnology Co. Ltd. Joint Research Center, Changsha, Hunan
410008, China.; Department of Sports Medicine, Xiangya Hospital, Central South
University, Changsha, Hunan 410008, China.; Department of Orthopedics, Movement
System Injury and Repair Research Center, Xiangya Hospital, Central South
University, Changsha, Hunan 410008, China.; Xiangya School of Nursing, Central
South University, Changsha, Hunan 410013, China.; Department of Orthopedics,
Movement System Injury and Repair Research Center, Xiangya Hospital, Central South
University, Changsha, Hunan 410008, China.; Angmedicine Research Center of Central
South University, Changsha, Hunan 410008, China.; Xiangya Hospital of Central South
University-Amcan Pharmaceutical Biotechnology Co. Ltd. Joint Research Center,
Changsha, Hunan 410008, China.; Department of Orthopedics, Movement System Injury
and Repair Research Center, Xiangya Hospital, Central South University, Changsha,
Hunan 410008, China.; Department of Sports Medicine, Xiangya Hospital, Central
South University, Changsha, Hunan 410008, China.; National Clinical Research Center
for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha,
410008, China.; Department of Orthopedics, Movement System Injury and Repair Res
arch Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008,
China.; Department of Orthopedics, Movement System Injury and Repair Research
Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.;
Institute of Integrative Medicine, Xiangya Hospital, Central South University,
Changsha, Hunan 410008, China.; Angmedicine Research Center of Central South
University, Changsha, Hunan 410008, China.; Xiangya Hospital of Central South
University-Amcan Pharmaceutical Biotechnology Co. Ltd. Joint Research Center,
Changsha, Hunan 410008, China.; Department of Clinical Laboratory, Xiangya
Hospital, Central South University, Changsha, Hunan 410008, China.; Xiangya School
of Nursing, Central South University, Changsha, Hunan 410013, China.; Department of
Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital,
Central South University, Changsha, Hunan 410008, China. huixie@csu.edu.cn.;
Department of Sports Medicine, Xiangya Hospital, Central South University,
Changsha, Hunan 410008, China.; National Clinical Research Center for Geriatric
Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008,
China.; Hunan Key Laboratory of Organ Injury, Aging and Regenerative Medicine,
Changsha, Hunan 410008, China.; Hunan Key Laboratory of Bone Joint Degeneration and
Injury, Changsha, Hunan 410008, China.
PY - 2020
ST - Ångstrom-scale silver particle-embedded carbomer gel promotes wound healing
T2 - Science advances
TI - Ångstrom-scale silver particle-embedded carbomer gel promotes wound healing
VL - 6
Y2 - 10
ID - 10014
ER -
TY - JOUR
AB - To explore the physiological mechanism of silver for the clinical application
of silver-containing biomaterials and biomedical devices, the plasma immersion ion
implantation method was used to prepare silver-doped titanium samples in this work.
The findings disclosed that with the increase of the introduced Ag amount, the
micro galvanic effects formed by silver and the titanium matrix became stronger,
which could stimulate the opening of voltage-gated calcium channels in macrophages
to facilitate Ca2+ influx. The increase of intracellular Ca2+ concentration could
induce macrophages to polarize towards the M1 phenotype by activating the Ca2+-
depen-dent PKC-NF-kappa B signaling pathway, while it was more favorable to the
polarization of M2 macrophages by improving the synthesis and secretion of calcium-
dependent PGE2. Therefore, with the increase of Ag amount, the micro-galvanic
effect was enhanced, and the anti-inflammatory responses of macrophages on samples
were improved. Furthermore, the co-cultured bone marrow mesenchymal stem cells
could inhibit the Ca2+ influx of the co-cultured macrophages to down-regulate the
Ca2+-dependent PKC-NF-kappa B signaling pathway in macrophages and secrete PGE2 to
promote the polarization of M2 macrophages, which weaken the inflammatory response.
Our results indicate that the immune response can be regulated by the micro-
galvanic effects of silver and titanium matrix, and provide new insights into
designing and developing the silver-containing biomaterials with a desirable
biological response.
AN - rayyan-553780299
AU - Chen, L.
AU - Wang, D. H.
AU - Liu, X. D.
AU - Yan, B. C.
AU - Zhang, H. F.
AU - Zhang, X. M.
AU - Qiao, Y. Q.
AU - Qiu, J. J.
AU - Liu, X. Y.
DO - 10.1016/j.cej.2021.131068
KW - Titanium
Macrophages
PY - 2022
SN - 1385-8947 1873-3212
ST - Micro-galvanic effects of silver-containing titanium implants regulate the
immune responses via activating voltage-gated calcium channels in macrophages
T2 - CHEMICAL ENGINEERING JOURNAL
TI - Micro-galvanic effects of silver-containing titanium implants regulate the
immune responses via activating voltage-gated calcium channels in macrophages
VL - 428
Y2 - 1 y3 - 15
ID - 8331
ER -
TY - JOUR
AB - Ds-block elements have been gaining increasing attention in the field of
biomaterials modification, owing to their excellent biological properties, such as
antibiosis, osteogenesis, etc. However, their function mechanisms are not well
understood and conflicting conclusions were drawn by previous studies on this
issue, which are mainly resulted from the inconsistent experimental conditions. In
this work, three most widely used ds-block elements, copper, zinc, and silver were
introduced on titanium substrate by plasma immersion ion implantation method to
investigate the rule of ds-block elements in the immune responses. Results showed
that the implanted samples could decrease the inflammatory responses compared with
Ti sample. The trend of anti-inflammatory effects of macrophages on samples was in
correlation with cellular ROS levels, which was induced by the implanted
biomaterials and positively correlated with the number of valence electrons of ds-
block elements. The co-culture experiments of macrophages and bone marrow
mesenchymal stem cells showed that these two kinds of cells could enhance the anti-
inflammation and osteogenesis of samples by the paracrine manner of PGE2. In
general, in their steady states on titanium substrate (Cu2+, Zn2+, Ag), the ds-
block elements with more valence electrons exhibit better anti-inflammatory and
osteogenic effects. Moreover, molecular biology experiments indicate that the PGE2-
related signaling pathway may contribute to the desired immunoregulation and
osteoinduction capability of ds-block elements. These findings suggest a
correlation between the number of valence electrons of ds-block elements and the
relevant biological responses, which provides new insight into the selection of
implanted ions and surface design of biomaterials. © 2020 [The Author/The Authors]
AN - rayyan-553780300
AU - Chen, L.
AU - Wang, D.
AU - Qiu, J.
AU - Zhang, X.
AU - Liu, X.
AU - Qiao, Y.
DO - 10.1016/j.bioactmat.2020.08.001
IS - 1
KW - Ds-block element
Immune response
Molecular mechanism
Osteoimmunology
Valence electron
copper
copper zinc superoxide dismutase
gamma interferon
inducible nitric oxide synthase
interleukin 1 receptor
interleukin 1beta
interleukin 4
interleukin 6
prostaglandin E2
silver nanoparticle
titanium
zinc
air pouch
animal cell
animal experiment
animal tissue
Article
bone development
bone marrow derived mesenchymal stem cell
bone tissue
cell adhesion
cell density
cell proliferation
cell spreading
cell structure
coculture
cytokine production
cytotoxicity
field emission scanning electron microscopy
flow cytometry
fluorescence activated cell sorting
fluorescence microscopy
immune response
immunofluorescence
immunoregulation
inductively coupled plasma atomic emission spectrometry
macrophage
male
microscopy
mineralization
mouse
nonhuman
photoelectron spectroscopy
RAW 264.7 cell line
surface property
tumor associated leukocyte
zeta potential
Titanium
PY - 2021
SP - 191-207
ST - Synergistic effects of immunoregulation and osteoinduction of ds-block
elements on titanium surface
T2 - Bioactive Materials
TI - Synergistic effects of immunoregulation and osteoinduction of ds-block
elements on titanium surface
85089337798&doi=10.1016%2fj.bioactmat.2020.08.001&partnerID=40&md5=5ec9743ba308f8e0
7a17883e6b9bc131
VL - 6
ID - 8332
ER -
TY - JOUR
AB - Lanthanide-doped sodium yttrium fluoride (NaYF4) nanoparticles exhibit novel
optical properties which make them be widely used in various fields. The extensive
applications increase the chance of human exposure to these nanoparticles and thus
raise deep concerns regarding their riskiness. In the present study, we have
synthesized europium doped NaYF4 (NaYF4:Eu3+) nanoparticles with three diameters
and used endothelial cells (ECs) as a cell model to explore the potential toxic
effect. The cell viability, cytomembrane integrity, cellular uptake, intracellular
localization, intracellular reactive oxygen species (ROS), mitochondrial membrane
potential (MMP), apoptosis detection, caspase-3 activity and expression of
inflammatory gene were studied. The results indicated that these nanoparticles
could be uptaken into ECs and decrease the cell viability, induce the intracellular
lactate dehydrogenase (LDH) release, increase the ROS level, and decrease the cell
MMP in a size-dependent manner. Besides that, the cells were suffered to apoptosis
with the caspase-3 activation, and the inflammation specific gene expressions
(ICAM1 and VCAM1) were also increased. Our results suggest that the damage pathway
may be related to the ROS generation and mitochondrial damage. The results provide
novel evidence to elucidate their toxicity mechanisms and may be helpful for more
rational applications of these compounds in the future. (C) 2014 Elsevier B.V. All
rights reserved.
AN - rayyan-553780307
AU - Chen, S. Z.
AU - Zhang, C. M.
AU - Jia, G.
AU - Duan, J. L.
AU - Wang, S. X.
AU - Zhang, J. C.
DO - 10.1016/j.msec.2014.07.029
PY - 2014
SN - 0928-4931 1873-0191
SP - 330-342
ST - Size-dependent cytotoxicity of europium doped NaYF4 nanoparticles in
endothelial cells
TI - Size-dependent cytotoxicity of europium doped NaYF4 nanoparticles in
endothelial cells
VL - 43
Y2 - 10 y3 - 1
ID - 8339
ER -
TY - JOUR
AB - Rationale: Inflammatory osteolysis, characterized by abundant immune cell
infiltration and osteoclast (OC) formation, is a common complication induced by
bacterial products and/or wear particles at the bone-prosthesis interface that
severely reduces long-term stability after implantation. Molecular nanoclusters are
ultrasmall particles with unique physicochemical and biological properties that
have great potential as theranostic agents for treating inflammatory
diseases.Methods: In this study, heterometallic PtAu2 nanoclusters with sensitive
nitric oxide-responsive phosphorescence turn-on characteristics and strong binding
interactions with cysteine were designed, making them desirable candidates for the
treatment of inflammatory osteolysis.Results: PtAu2 clusters exhibited satisfactory
biocompatibility and cellular uptake behavior, with potent antiinflammatory and
anti-OC activities in vitro. In addition, PtAu2 clusters alleviated
lipopolysaccharide-induced calvarial osteolysis in vivo and activated nuclear
factor erythroid 2-related factor 2 (Nrf2) expression by disrupting its association
with Kelch-like ECH-associated protein 1 (Keap1), thereby upregulating the
expression of endogenous anti-inflammatory and anti-oxidative products.Conclusion:
Through the rational design of novel heterometallic nanoclusters that activate the
endogenous anti-inflammatory system, this study provides new insights into the
development of multifunctional molecular therapeutic agents for inflammatory
osteolysis and other inflammatory diseases.
AN - rayyan-553780308
AU - Chen, X. Z.
AU - Cao, X. K.
AU - Zheng, D. S.
AU - Li, C.
AU - Chen, Y.
AU - Kong, K. Y.
AU - Xu, W. F.
AU - Shi, B.
AU - Chen, X. W.
AU - Dai, F. R.
AU - Zhang, S. Y.
DO - 10.7150/thno.80514
IS - 3
KW - Osteolysis
PY - 2023
SN - 1838-7640
SP - 1010-1027
ST - Ultrasmall PtAu2 nanoclusters activate endogenous anti-inflammatory and anti-
oxidative systems to prevent inflammatory osteolysis
T2 - THERANOSTICS
TI - Ultrasmall PtAu2 nanoclusters activate endogenous anti-inflammatory and anti-
oxidative systems to prevent inflammatory osteolysis
VL - 13
ID - 8340
ER -
TY - JOUR
AB - Bacterial infections are often treated inadequately. Sepsis, being one of its
most severe forms, is a multi-layered, life-threatening syndrome induced by rampant
immune responses, like cytokine storms, that leads to high morbidity and death of
infected patients. Particularly, the current increment in resistant bacterial
strains and the lack of creative antibiotics to counter such menace are central
reasons to the worsening of the situation. To avoid the said crisis, the
antimicrobial peptides (AMPs) were used to target cell wall components, such as
lipopoly-saccharides (LPS), seems to have the most promise. These combine the
ability of broad-spectrum bactericidal activity with low potential for induction of
resistance. Inhibition of cytokine storms induced by activated immune cells has
been considered a feasible treatment for in sepsis. One of the therapeutic
approaches widely utilized in inducing apoptosis in inflammatory cells is the use
of tumor necrosis factor (TNF)-related apoptosisinducing ligands (TRAIL), which
trigger an extrinsic apoptotic pathway via death receptors. Herein, we report TRAIL
encapsulated in a bactericidal polypeptide-crosslinked nanogel that suppressed
Klebsiella pneumoniae infection and overactive macrophages. Of interest, nanogel
and TRAIL-nanogel treatments were more toxic towards LPS-activated cells than to
naive cells in cell viability assays. Treatment with TRAIL-nanogel significantly
prolonged survival in septic mice and reduced bacterial numbers in circulation. As
such, TRAIL-nanogel may be promising as a therapeutic agent for treating bacteria-
infected diseases.
AN - rayyan-553780309
AU - Chen, Y. F.
AU - Chen, G. Y.
AU - Chang, C. H.
AU - Su, Y. C.
AU - Chen, Y. C.
AU - Jiang, Y. S.
AU - Jan, J. S.
DO - 10.1016/j.msec.2019.04.023
KW - Sepsis
PY - 2019
SN - 0928-4931 1873-0191
SP - 85-95
ST - TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased
anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment
T2 - MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
TI - TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased
anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment
VL - 102
Y2 - 9
ID - 8341
ER -
TY - JOUR
AB - Today, earphones have almost been owned by everyone. However, wearing
earphones for a long time can cause two serious problems: (1) irreversible damage
to hearing; (2) rapid proliferation of bacteria in the ear canal. Herein, an
earphone modification strategy is developed for the first time, to reduce hearing
loss and inhibit bacteria simultaneously. This earphone is equipped with a high
purity yellow light (YL) light-emitting diode chip developed by our university.
Then, the surface of the earphone is loaded with porous zinc oxide and silver
nanoparticle composite material (ZnO-Ag) that can respond to the YL. Under the
excitation of YL, the porous ZnO-Ag can release reactive oxygen species with strong
antibacterial activity. More importantly, we discover that YL can reduce the
expression of matrix metalloproteinase-3, the secretion of inflammatory factors,
and apoptosis in the cochlea, thereby effectively reduce hearing loss. [Figure not
available: see fulltext.] © 2022, Tsinghua University Press.
AN - rayyan-553780312
AU - Cheng, H.
AU - Liu, H.
AU - Liu, Z.
AU - Xu, Z.
AU - Liu, X.
AU - Jia, S.
AU - He, C.
AU - Liu, S.
AU - Zhang, J.
AU - Wang, X.
DO - 10.1007/s12274-022-4240-7
IS - 7
KW - antibacterial activity
earphone
hearing loss
yellow light
ZnO
Bacteria
Cell death
Zinc oxide
Diode chips
Ear canal
Hearing loss
High purity
Irreversible damage
Lightemitting diode
Material-based
Responsive materials
Yellow light
Audition
PY - 2022
SP - 6297-6305
ST - Yellow responsive material based modification to reduce earphone induced
Infection and hearing loss
T2 - Nano Research
TI - Yellow responsive material based modification to reduce earphone induced
Infection and hearing loss
85127634912&doi=10.1007%2fs12274-022-4240-
7&partnerID=40&md5=d7afd9124bb1ad85e3fb6fa8324c5eab
VL - 15
ID - 8344
ER -
TY - COMP
AB - Brucella vaccination is one of the most important strategies for controlling
brucellosis in livestock. The A19 strain was the effective vaccine used to control
brucellosis in China. However, the characteristics of physiological and attenuated
virulence of the A19 strain are not investigated in detail. In this study, we
compared the phenotypic characteristics of the A19 to the wild-type strain S2308.
Virulence test showed that the A19 was significantly attenuated at chronic
infection stage in infected mouse model. In growth analysis, the A19 exhibited a
quick growth at exponential phase and premature at stationary phase. The
inflammatory response of macrophages infected by the A19 was detected using TaqMan
qPCR assay, indicating that the inflammatory level of the A19-infected macrophages
was higher than that of the S2308 infection. Cell death analysis showed that the
A19 was not cytotoxic for macrophages. Cell infection showed that the A19 reduced
its ability to invade, survive and traffic within host cells, and the intracellular
A19 hardly excludes lysosome-associated marker LAMP-1, suggesting that the A19
can't escape the lysosome degradation within host cells. In further study, the
sensitivity test exhibited that the A19 is more sensitive to stress and
bactericidal factors than the S2308 strain, Western blot and silver staining
analysis exhibited that the A19 has a different expression pattern of OMPs and
reduces LPS O-antigen expression relative to the S2308 strain. Those data give us a
more detailed understanding about the A19 vaccine strain, which will be beneficial
for improvement of current Brucella vaccine and overcoming its defects.
AN - rayyan-553782125
AU - Cheng, Z.
AU - Li, Z.
AU - Yin, Y.
AU - Lian, Z.
AU - Abdelgawad, H. A.
AU - Hu, H.
AU - Guan, X.
AU - Zuo, D.
AU - Cai, Y.
AU - Ding, C.
AU - Wang, S.
AU - Li, T.
AU - Qi, J.
AU - Tian, M.
AU - Yu, S.
CY - Netherlands
DO - 10.1016/j.vetmic.2021.109007
J2 - Vet Microbiol
KW - Animals
Brucella Vaccine/*immunology
Brucella abortus/classification/genetics/*immunology/pathogenicity
Brucellosis/prevention & control/*veterinary
Chronic Disease
HeLa Cells
Humans
Macrophages/*immunology/*microbiology
Mice
Mice, Inbred BALB C
Phenotype
RAW 264.7 Cells
Vaccines, Attenuated
Virulence
Vaccination
LA - eng
N1 - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural
Sciences (CAAS), Shanghai, 200241, China; College of Animal Science and Technology,
Shandong Agricultural University, Taian, 271018, China.; Shanghai Veterinary
Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai,
200241, China.; Shanghai Veterinary Research Institute, Chinese Academy of
Agricultural Sciences (CAAS), Shanghai, 200241, China.; Shanghai Veterinary
200241, China.; College of Animal Science and Technology, Shandong Agricultural
University, Taian, 271018, China.; Shanghai Veterinary Research Institute, Chinese
Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China.; Shanghai
Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS),
Shanghai, 200241, China.; Shanghai Veterinary Research Institute, Chinese Academy
of Agricultural Sciences (CAAS), Shanghai, 200241, China.; Shanghai Veterinary
Agricultural Sciences (CAAS), Shanghai, 200241, China. Electronic address:
tianmx530@126.com.; Shanghai Veterinary Research Institute, Chinese Academy of
Agricultural Sciences (CAAS), Shanghai, 200241, China. Electronic address:
yus@shvri.ac.cn.
PY - 2021
SP - 109007
ST - Characteristics of Brucella abortus vaccine strain A19 reveals its potential
mechanism of attenuated virulence
T2 - Veterinary microbiology
TI - Characteristics of Brucella abortus vaccine strain A19 reveals its potential
mechanism of attenuated virulence
VL - 254
Y2 - 3
ID - 10036
ER -
TY - JOUR
AB - Antibiotic era is coming to the end, becouse of the wrong selecting of
antibiotics and combination therapy and the mutation skills of bacteria. In recent
years, the use of nanosilver particles for therapeutic purposes has become more
relevant. These particles have an antibacterial effect and a fairly good effect on
local inflammatory processes. We made suspension of silver nanoparticles size 50 nm
by the physic method and introduced into laboratory rats. Our studies demonstrates
that argentium nano-particles can be administered in physical (non-chemical)
pathways. It does not have LD50, however, its consumption with high doses for
prolonged period of time leads to histomorphological changes in healthy tissues. ©
2019, Advanced Scientific Research. All rights reserved.
AN - rayyan-553780316
AU - Chichiveishvili, N.
AU - Tavkhelidze, T.
AU - Khubulava, S.
AU - Pitchkhaia, G.
AU - Kristesashvili, J.
AU - Kacadze, D.
IS - 1
KW - Drug toxicity.a
nanoparticle
silver nanoparticle
animal experiment
animal model
animal tissue
Article
body movement
chronic inflammation
chronic toxicity
controlled study
defecation
edema
emphysema
erythrocyte
feeding
histopathology
LD50
lethality
liver congestion
lung congestion
morphological trait
nonhuman
particle size
rat
Lethal Dose 50
Rats
PY - 2019
SP - 713-715
ST - Study of LD50 and chronic toxicity of suspension of silver nanoparticles size
50 nm in laboratory rats
T2 - International Journal of Pharmaceutical Research
TI - Study of LD50 and chronic toxicity of suspension of silver nanoparticles size
50 nm in laboratory rats
85065082999&partnerID=40&md5=a3ede26ff901eece5e5c05fdc8af4762
VL - 11
ID - 8348
ER -
TY - CPAPER
AB - Biocompatibility of photoluminescent silicon nanocrystals was tested using
standard cytotoxicity protocols with murine macrophage cell line RAW 264.7. We
investigated the cytotoxicity and inflammatory responses of cells exposed to
silicon nanocrystals by several biological endpoints. Cell death ratio,
morphological changes, and the levels of nitric oxide production were studied.
Spatial position of the nanoparticles relative to cell body was investigated using
fluorescent microscopy. No statistically significant cytotoxicity or inflammatory
response was detected with autoclaved silicon nanoparticles at concentrations up to
20 mu g/ml in RAW 264.7 cells. The present study of murine macrophages, exposed to
autoclaved silicon nanocrystals, will help define safety requirements for
comparable nanoparticle biomedical applications.
AN - rayyan-553780319
AU - Choi, J. H.
AU - Zhang, Q.
AU - Hitchins, V. M.
AU - Wang, N. S.
AU - Reipa, V.
DO - 10.1117/12.734222
KW - Silicon
Silicones
PY - 2007
T2 - NANOENGINEERING: FABRICATION, PROPERTIES, OPTICS, AND DEVICES IV
TI - Cytotoxicity of the photoluminescent silicon nanocrystals
VL - 6645
ID - 8351
ER -
TY - JOUR
AB - Photoluminescent silicon nanoparticles have a bright and stable fluorescence
and are promising candidates for bio-imaging, cell staining and drug delivery. With
increasing development of nanotechnology applications for biomedicine, an
understanding of the potential toxicity of nanoparticles is needed to assess safety
concerns for clinical applications. The objective of this study was to compare
biological responses of silicon nanoparticles (SNs, 3 nm diameter) with silicon
microparticles (SMs, similar to 100-3000 nm diameter) in cultured murine
macrophages (RAW 264.7) using standard protocols for assessing cytotoxicity/cell
viability and inflammatory responses developed for micron-sized particles. SNs and
SMs were exposed to macrophages with and without addition of endotoxin
lipopolysaccharide (LIPS), a positive inducer of tumor necrosis factor-alpha (TNF-
alpha), interleukin 6 (IL-6), and nitric oxide (NO). Cytotoxicity was assayed using
the dye exclusion and MTT assays. Cell supernatants were assayed for production
TNF-alpha, IL-6 and NO. SNs at concentrations <= 20 mu g ml(-1) exhibited no
cytotoxicity or inflammatory responses; however, SNs and SMs > 20 and 200 mu g ml(-
1), respectively, increased cytotoxicity compared with controls. SMs induced
concentration-related increases in TNF-alpha and IL-6 production; in contrast, the
production of these cytokines was shown to decrease with increasing concentrations
of SNs. NO production was not induced by SNs or SMs alone. Fluorescence microscopy
demonstrated that SNs were associated with the macrophages, either internalized or
attached to cell membranes. In conclusion, evaluating differences in biological
responses for nanoparticles compared with microparticles of the same material may
help improve tests to assess biological responses of nanoparticles that may be used
in biomedical applications. Copyright (C) 2008 John Wiley & Sons, Ltd.
AN - rayyan-553780320
AU - Choi, J.
AU - Zhang, Q.
AU - Reipa, V.
AU - Wang, N. S.
AU - Stratmeyer, M. E.
AU - Goering, P. L.
DO - 10.1002/jat.1382
IS - 1
KW - Particle Size
Silicon
Macrophages
Silicones
PY - 2009
SN - 0260-437X 1099-1263
SP - 52-60
ST - Comparison of cytotoxic and inflammatory responses of photoluminescent
silicon nanoparticles with silicon micron-sized particles in RAW 264.7 macrophages
TI - Comparison of cytotoxic and inflammatory responses of photoluminescent
silicon nanoparticles with silicon micron-sized particles in RAW 264.7 macrophages
VL - 29
Y2 - 1
ID - 8352
ER -
TY - JOUR
AB - Nanoparticles (range under 100 nm) prepared by different technology modes
including physical, chemical, biological have many applications. Like in the same
way silver nanoparticles are used for different beneficial actions like
antimicrobial- antibacterial, antifungal and antiviral, anti-inflammatory,
anticancer, water treatment, cosmetics, and in the textiles industry. As silver
nanoparticles have shown wide application by different mechanisms against various
pathophyisiological conditions. To maintain safety under their use, the study of
the toxicity of silver nanoparticles has become more important. Health agencies
like WHO, NIOSH, EPA, EFSA & EU have issued guidelines for unrisky exposure limit
of silver nanopartricles in drinking water, food and breathing. The main purpose of
this article is to summarize genotoxicity, cytotoxicity, neurotoxicity,
reproductive toxicity of silver nanoparticles in both in vitro and in vivo studies
focused on mechanism and methods of detection. The main mechanism of silver
nanoparticles toxicity involves disruption of the mitochondrial respiratory chain,
which results in the generation of ROS and the stoppage of ATP synthesis which
further leads to a cascade of toxic events. ROS production measured by the
technique like flow cytometry using DCFHDA dye and other method includes a confocal
microscope, lipid peroxidation, etc. Different assay techniques used for evaluation
of different kind of toxicities such as the comet assay, MTT assay, and
histological assay, are also discussed. © 2022 Informa UK Limited, trading as
AN - rayyan-553780321
AU - Choudhary, A.
AU - Singh, S.
AU - Ravichandiran, V.
DO - 10.1080/15376516.2022.2064257
IS - 9
KW - cytotoxicity
genotoxicity
neurotoxicity
reproductive toxicity
ROS
Adenosine Triphosphate
Antifungal Agents
Antiviral Agents
Drinking Water
Metal Nanoparticles
Silver
drinking water
silver
silver nanoparticle
antifungal agent
antiinfective agent
antivirus agent
metal nanoparticle
acrosome
breathing
cell line
cell membrane
comet assay
DNA damage
female genital system
flow cytometry
histopathology
in vitro study
in vivo study
lipid peroxidation
mitochondrion
MTT assay
practice guideline
respiratory chain
Review
sperm quality
synthesis
toxicity assay
PY - 2022
SP - 650-661
ST - Toxicity, preparation methods and applications of silver nanoparticles: an
update
T2 - Toxicology Mechanisms and Methods
TI - Toxicity, preparation methods and applications of silver nanoparticles: an
update
85129616600&doi=10.1080%2f15376516.2022.2064257&partnerID=40&md5=8d9802527dd1e96d02
7e782a7ca01c19
VL - 32
ID - 8353
ER -
TY - JOUR
AB - Wounds associated with diabetes mellitus are the most severe co-morbidities,
which could be progressed to cause cell necrosis leading to amputation. Statistics
on the recent status of the diabetic wounds revealed that the disease affects 15%
of diabetic patients, where 20% of them undergo amputation of their limb.
Conventional therapies are found to be ineffective due to changes in the molecular
architecture of the injured area, urging novel deliveries for effective treatment.
Therefore, recent researches are on the development of new and effective wound care
materials. Literature is evident in providing potential tools in topical drug
delivery for wound healing under the umbrella of nanotechnology, where nano-
scaffolds and nanofibers have shown promising results. The nano-sized particles are
also known to promote healing of wounds by facilitating proper movement through the
healing phases. To date, focuses have been made on the efficacy of silver
nanoparticles (AgNPs) in treating the diabetic wound, where these nanoparticles are
known to exploit potential biological properties in producing anti-inflammatory and
antibacterial activities. AgNPs are also known to activate cellular mechanisms
towards the healing of chronic wounds; however, associated toxicities of AgNPs are
of great concern. This review is an attempt to illustrate the use of AgNPs in wound
healing to facilitate this delivery system in bringing into clinical applications
for a superior dressing and treatment over wounds and ulcers in diabetes patients.
© 2020
AN - rayyan-553780322
AU - Choudhury, H.
AU - Pandey, M.
AU - Lim, Y. Q.
AU - Low, C. Y.
AU - Lee, C. T.
AU - Marilyn, T. C. L.
AU - Loh, H. S.
AU - Lim, Y. P.
AU - Lee, C. F.
AU - Bhattamishra, S. K.
AU - Kesharwani, P.
AU - Gorain, B.
DO - 10.1016/j.msec.2020.110925
KW - Antibacterial property
Clinical aspects
Diabetic wound
Effective dressing
Nanotechnology
Silver nanoparticle
Bandages
Diabetic Foot
Humans
Metal Nanoparticles
Silver
Wound Healing
Artificial limbs
Cell death
Diseases
Metal nanoparticles
Particle size
antiinfective agent
metal nanoparticle
silver
Biological properties
Cellular mechanisms
Clinical application
Molecular architecture
Nano-sized particles
Topical drug deliveries
bandage
chemistry
diabetic foot
human
metabolism
pathology
wound healing
PY - 2020
ST - Silver nanoparticles: Advanced and promising technology in diabetic wound
therapy
T2 - Materials Science and Engineering C
TI - Silver nanoparticles: Advanced and promising technology in diabetic wound
therapy
85083296998&doi=10.1016%2fj.msec.2020.110925&partnerID=40&md5=d14b2a2aeecd13dac87d8
e358fcee115
VL - 112
ID - 8354
ER -
TY - JOUR
AB - Gymnema sylvestre is a plant that is enriched in bioactive compounds. In
particular, gymnemic acids (GA) and phenolic compounds (PC) are pharmaceutically
important. There is a commercial demand for naturally occurring bioactive
compounds, but their availability is limited due to geographical and seasonal
variations. The elicitation approach can enhance the biosynthesis of phytochemicals
during in vitro culture of G. sylvestre. Here, to further improve gymnemic acid II
(GA II) and phenolic compounds (PC) production by G. sylvestre, cell suspension
cultures (CSC), which has attracted attention for the production of essential
phytochemicals, was explored using copper oxide nanoparticles (CuO NPs). Callus was
obtained on MS medium containing 2,4-dichlorophenoxyacetic acid, kinetin,
phytoagar, and sucrose. Agar-free MS medium was used to initiate CSC, which was
treated with three concentrations of CuO NPs (1, 3 or 5 mg/L). Treatment for 48 h
with 3 mg/L CuO NPs resulted in the greatest yields of GA II, total phenolics, and
flavonoids. The cultures also displayed pronounced antioxidant, antidiabetic, anti-
inflammatory, antibacterial, antifungal, and anticancer activities. The use of CuO
NPs (3 mg/L) significantly increased the production of GA II (nine-fold) and PC
compared to unamended CSC. We propose that CSC and use of nanoparticles (NPs) as a
new generation of elicitors, offer a suitable prospect for the production of
bioactive compounds.
AN - rayyan-553780324
AU - Chung, I. M.
AU - Rajakumar, G.
AU - Subramanian, U.
AU - Venkidasamy, B.
AU - Thiruvengadam, M.
DO - 10.3390/app9102165
IS - 10
PY - 2019
SN - 2076-3417
ST - Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds
Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br
T2 - APPLIED SCIENCES-BASEL
TI - Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds
Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br
VL - 9
Y2 - 5 y3 - 2
ID - 8356
ER -
TY - JOUR
AB - The preparation of graphene-based nanomaterials (GBNs) with appropriate
stability and biocompatibility is crucial for their use in biomedical applications.
In this work, three GBNs differing in size and/or functionalization have been
synthetized and characterized, and their in vitro biological effects were compared.
Pegylated graphene oxide (GO-PEG, 200-500 nm) and flavin mononucleotide-stabilized
pristine graphene with two different sizes (PG-FMN, 200-400 nm and 100-200 nm) were
administered to macrophages, chosen as cellular model due to their key role in the
processing of foreign materials and the regulation of inflammatory responses. The
results showed that cellular uptake of GBNs was mainly influenced by their lateral
size, while the inflammatory potential depended also on the type of
functionalization. PG-FMN nanomaterials (both sizes) triggered significantly higher
nitric oxide (NO) release, together with some intracellular metabolic changes,
similar to those induced by the prototypical inflammatory stimulus LPS. NMR
metabolomics revealed that macrophages incubated with smaller PG-FMN displayed
increased levels of succinate, itaconate, phosphocholine and phosphocreatine,
together with decreased creatine content. The latter two variations were also
detected in cells incubated with larger PG-FMN nanosheets. On the other hand, GO-
PEG induced a decrease in the inflammatory metabolite succinate and a few other
changes distinct from those seen in LPS-stimulated macrophages. Assessment of TNF-
alpha secretion and macrophage surface markers (CD80 and CD206) further
corroborated the low inflammatory potential of GO-PEG. Overall, these findings
revealed distinct phenotypic and metabolic responses of macrophages to different
GBNs, which inform on their immunomodulatory activity and may contribute to guide
their therapeutic applications.
AN - rayyan-553780325
AU - Cicuendez, M.
AU - Fernandes, M.
AU - Ayan-Varela, M.
AU - Oliveira, H.
AU - Feito, M. J.
AU - Diez-Orejas, R.
AU - Paredes, J. I.
AU - Villar-Rodil, S.
AU - Vila, M.
AU - Portoles, M. T.
AU - Duarte, I. F.
DO - 10.1016/j.colsurfb.2019.110709
KW - Macrophages
PY - 2020
SN - 0927-7765 1873-4367
ST - Macrophage inflammatory and metabolic responses to graphene-based
nanomaterials differing in size and functionalization
T2 - COLLOIDS AND SURFACES B-BIOINTERFACES
TI - Macrophage inflammatory and metabolic responses to graphene-based
nanomaterials differing in size and functionalization
VL - 186
Y2 - 2
ID - 8357
ER -
TY - JOUR
AB - Fucoidan is a polysaccharide built from L-fucose molecules. The main source
of this polysaccharide is the extracellular matrix of brown seaweed (Phaeophyta),
but it can be also isolated from invertebrates such as sea urchins (Echinoidea) and
sea cucumbers (Holothuroidea). Interest in fucoidan is related to its broad
biological activity, including possible antioxidant, anti-inflammatory, antifungal,
antiviral or antithrombotic effects. The potential application of fucoidan in the
pharmaceutical technology is also due to its ionic nature. The negative charge of
the molecule results from the presence of sulfate residues in the C-2 and C-4
positions, occasionally in C-3, allowing the formation of complexes with other
oppositely charged molecules. Fucoidan is non-toxic, biodegradable and
biocompatible compound approved by Food and Drug Administration (FDA) as Generally
Recognized As Safe (GRAS) category as food ingredient. Fucoidan plays an important
role in the pharmaceutical technology, so in this work aspects concerning its
pharmaceutical characteristics and designing of various dosage forms
(nanoparticles, liposomes, microparticles, and semisolid formulations) with
fucoidan itself and with its combinations with other polymers or components that
give a positive charge were reviewed. Advantages and limitations of fucoidan
utilization in the pharmaceutical technology were also discussed. © 2019 by the
authors.
AN - rayyan-553780326
AU - Citkowska, A.
AU - Szekalska, M.
AU - Winnicka, K.
DO - 10.3390/md17080458
IS - 8
KW - Fucoidan
Fucospheres
Marine-derived
Multifunctional polymer
Pharmaceutical formulations
Polysaccharide
Animals
Biocompatible Materials
Chemistry, Pharmaceutical
Phaeophyta
Polysaccharides
Sea Cucumbers
Sea Urchins
Seaweed
Technology, Pharmaceutical
chitosan
cisplatin
curcumin
doxorubicin
fucoidin
heparin
liposome
methotrexate
nanoparticle
polymer
silver nitrate
tissue plasminogen activator
biomaterial
polysaccharide
anticoagulation
apoptosis
biocompatibility
cross linking
cytokine production
drug design
drug dosage form
drug formulation
drug isolation
drug mechanism
drug safety
drug structure
Food and Drug Administration
Fucus vesiculosus
genotoxicity
human
hydrogel
hydrophilicity
Laminaria japonica
nonhuman
particle size
pH
Review
temperature
Undaria pinnatifida
viscosity
animal
brown alga
chemistry
isolation and purification
medicinal chemistry
pharmaceutics
procedures
sea cucumber
sea urchin
seaweed
Dosage Forms
PY - 2019
ST - Possibilities of fucoidan utilization in the development of pharmaceutical
dosage forms
T2 - Marine Drugs
TI - Possibilities of fucoidan utilization in the development of pharmaceutical
dosage forms
85070219424&doi=10.3390%2fmd17080458&partnerID=40&md5=60f227ce03ad04366db853d519874
fff
VL - 17
ID - 8358
ER -
TY - JOUR
AB - Anti-inflammatory molecules often display little affinity for inflamed
tissues, leading to low accumulation into this site of action (and inefficiency),
and high incidence of severe side effects. To face the problem, numerous strategies
have been proposed, i.e., chemical modifications to the drug molecule, and
engineering of drug nanocarriers. The later approach to the problem can result in
optimized drug biodistribution and concentration into the target region, thus
enhancing the anti-inflammatory effect while reducing the associated drug toxicity.
Such nanoparticulate systems offer remarkable possibilities when they are made of
biodegradable polymers, lipid-based structures, and/or inorganic particles. Recent
advances in the field have been devoted to the optimization of the in vivo fate and
effectiveness of these drug nanocarriers, e.g., passive targeting strategies based
on the functionalization of nanoparticle surface with special biomolecules. In this
contribution, we analyze the possibilities and future perspectives of nanoparticle
therapy in inflammatory processes. © 2012 Bentham Science Publishers.
AN - rayyan-553780327
AU - Clares, B.
AU - Ruiz, M. A.
AU - Gallardo, V.
AU - Arias, J. L.
DO - 10.2174/092986712800784676
IS - 19
KW - Arthritis
Enhanced permeation and retention effect
Inflammation
Ligand-mediated drug/gene delivery
Nanoparticle
Passive drug targeting
Animals
Humans
Nanoparticles
celecoxib
dexamethasone
diclofenac
gold nanoparticle
ibuprofen
inorganic compound
lipid
liposome
lutetium 177
methylprednisolone
nanocarrier
nanomaterial
nanoparticle
niosome
palmitic acid
phosphatidylcholine
poloxamer
polymer
polysorbate 80
prednisolone
radioisotope
silver nanoparticle
stearic acid
triacylglycerol
tripalmitin
tristearin
unindexed drug
biocompatibility
biodegradation
chemical modification
drug accumulation
drug blood level
drug distribution
drug formulation
drug half life
drug release
drug safety
drug stability
emulsion
human
hydrophilicity
hydrophobicity
in vivo study
inflammation
lipid bilayer
lipogenesis
liposomal delivery
microemulsion
molecule
nonhuman
particle size
polymerization
rabbit
review
PY - 2012
SP - 3203-3211
ST - Drug delivery to inflammation based on nanoparticles surface decorated with
biomolecules
T2 - Current Medicinal Chemistry
TI - Drug delivery to inflammation based on nanoparticles surface decorated with
biomolecules
84863682372&doi=10.2174%2f092986712800784676&partnerID=40&md5=91c6f2c60aa040fe3c072
0ef076c0b32
VL - 19
ID - 8359
ER -
TY - JOUR
AB - Large wounds are characterized by clinical and surgical challenges, requiring
numerous searches on demand for inexpensive biocompatible materials that produce
the best quality of cutaneous healing. Cellulose is a biopolymer of greatest
abundance, good biocompatibility and wide application due to its chemical and
physical properties. This study was aimed to develop and characterize low cost
membranes of wood cellulose nanofibrils for potential application as a wound
dressing in comparison to a commercial porous regenerating membrane. An inexpensive
mechanical method was used to defibrillate cellulose, and later the membranes were
obtained from the nanofibrils by filtering and drying under mild pressure. The
techniques employed for characterization included scanning electron microscopy,
physical testing, application in vivo and cost-effectiveness analysis. The
membranes presented promising physical properties for application as cutaneous
dressing, with characteristic translucency allowing the evaluation of the wound
without the need of removal and exchange of the dressing. Wood nanocellulose
dressing seems to be promising for wound care since it presented good adhesion to
the moist wound surfaces thus promoting the most efficient repitialization in the
first 4 days. No allergic reaction or inflammatory response to wood nanocellulose
dressings was observed. The wood nanocellulose membranes stand out as a potential
wound dressing, as it shows similar efficacy of bacterial cellulose dressing.
Besides the efficacy, the membrane developed in this study presents a simpler,
faster and cheaper manufacturing process, which may reduce the production cost by
99%.
AN - rayyan-553780328
AU - Claro, F. C.
AU - Jordao, C.
AU - de Viveiros, B. M.
AU - Isaka, L. J. E.
AU - Villanova, J. A.
AU - Magalhaes, W. L. E.
DO - 10.1007/s10570-020-03129-2
IS - 18
KW - Bandages
PY - 2020
SN - 0969-0239 1572-882X
SP - 10765-10779
ST - Low cost membrane of wood nanocellulose obtained by mechanical defibrillation
for potential applications as wound dressing
T2 - CELLULOSE
TI - Low cost membrane of wood nanocellulose obtained by mechanical defibrillation
for potential applications as wound dressing
VL - 27
Y2 - 12
ID - 8360
ER -
TY - JOUR
AB - BACKGROUND: In lung transplantation, ischemia-reperfusion injury associated
with mitochondrial damage can lead to graft rejection. Intact, exogenous
mitochondria provide a unique treatment option to salvage damaged cells within lung
tissue. METHODS: We developed a novel method to freeze and store allogeneic
mitochondria isolated from porcine heart tissue. Stored mitochondria were injected
into a model of induced ischemia-reperfusion injury using porcine ex-vivo lung
perfusion. Treatment benefits to immune modulation, antioxidant defense, and
cellular salvage were evaluated. These findings were corroborated in human lungs
undergoing ex-vivo lung perfusion. Lung tissue homogenate and primary lung
endothelial cells were then used to address underlying mechanisms. RESULTS:
Following cold ischemia, mitochondrial transplant reduced lung pulmonary vascular
resistance and tissue pro-inflammatory signaling and cytokine secretion. Further,
exogenous mitochondria reduced reactive oxygen species by-products and promoted
glutathione synthesis, thereby salvaging cell viability. These results were
confirmed in a human model of ex-vivo lung perfusion wherein transplanted
mitochondria decreased tissue oxidative and inflammatory signaling, improving lung
function. We demonstrate that transplanted mitochondria induce autophagy and
suggest that bolstered autophagy may act upstream of the anti-inflammatory and
antioxidant benefits. Importantly, chemical inhibitors of the MEK autophagy pathway
blunted the favorable effects of mitochondrial transplant. CONCLUSIONS: These data
provide direct evidence that mitochondrial transplant improves cellular health and
lung function when administered during ex-vivo lung perfusion and suggest the
mechanism of action may be through promotion of cellular autophagy. Data herein
contribute new insights into the therapeutic potential of mitochondrial transplant
to abate ischemia-reperfusion injury during lung transplant, and thus reduce graft
rejection.
AN - rayyan-553780329
AU - Cloer, C. M.
AU - Givens, C. S.
AU - Buie, L. K.
AU - Rochelle, L. K.
AU - Lin, Y. T.
AU - Popa, S.
AU - Shelton, R. V. M.
AU - Zhan, J. M.
AU - Zimmerman, T. R.
AU - Jones, B. G.
AU - Lesesne, Z.
AU - Hogan, S. S.
AU - Petersen, T. H.
DO - 10.1016/j.healun.2023.01.002
IS - 5
KW - Lung
PY - 2023
SN - 1053-2498 1557-3117
ST - Mitochondrial transplant after ischemia reperfusion promotes cellular salvage
and improves lung function during ex-vivo lung perfusion
T2 - JOURNAL OF HEART AND LUNG TRANSPLANTATION
TI - Mitochondrial transplant after ischemia reperfusion promotes cellular salvage
and improves lung function during ex-vivo lung perfusion
VL - 42
Y2 - 5
ID - 8361
ER -
TY - JOUR
AB - Plastics are one of the most preoccupying emerging pollutants. Macroplastics
released into the environment degrade into microplastics and nanoplastics. Because
of their small size, these micro and nano plastic particles can enter the food
chain and, in addition to their ecotoxicological effects, contaminate humans with
still unknown biological effects. Plastics being particulate pollutants, they are
handled in the human body by scavenger cells such as macrophages, which are
important players in the immune system. In order to get a better appraisal of the
effects of plastic particles on macrophages, we have studied the effects of
unmodified polystyrene particles of 0.1, 1 and 10 micrometers of diameter, by a
combination of proteomics and targeted validation experiments. Proteomics showed
important adaptive changes of the proteome in response to exposure to plastics,
with more than one third of the detected proteins showing a significance change in
their abundance in response to cell exposure to at least one plastic bead size.
These changes affected for example mitochondrial, lysosomal or cytoskeletal
proteins. Although an increase in the mitochondrial transmembrane potential was
detected in response to 10 micrometer beads, no alteration in cell viability was
observed. Similarly, no lysosomal dysfunction and no alteration in the phagocytic
capability of the cells was observed in response to exposure to plastics. When the
inflammatory response was examined, no increase in the secretion of tumor necrosis
factor or interleukin 6 was observed. Oppositely, the secretion of these cytokines
in response to lipopolysaccharide was observed after exposure to plastics, which
suggested a decreased ability of macrophages to respond to bacterial infection. In
conclusion, these results provide a better understanding of the responses of
macrophages to exposure to polystyrene particles of different sizes.
AN - rayyan-553780331
AU - Dalzon, B.
AU - Devcic, J.
AU - Diemer, H.
AU - Cianferani, S.
AU - Rabilloud, T.
DO - 10.1039/d2en00214k
IS - 8
KW - Proteomics
Proteome
Macrophages
PY - 2022
SN - 2051-8153 2051-8161
SP - 2827-2840
ST - Does size matter? A proteomics-informed comparison of the effects of
polystyrene beads of different sizes on macrophages
T2 - ENVIRONMENTAL SCIENCE-NANO
TI - Does size matter? A proteomics-informed comparison of the effects of
polystyrene beads of different sizes on macrophages
VL - 9
Y2 - 8 y3 - 11
ID - 8363
ER -
TY - JOUR
AB - From a toxicological point of view, nanomaterials are of interest; because -
on account of their great surface area relative to mass -they tend to be more
reactive than the bulk chemicals from which they are derived. They might in some
cases have the potential to damage DNA directly, or could act via the induction of
oxidative stress. The comet assay (single cell gel electrophoresis) is widely used
to measure DNA strand breaks and also oxidised bases, by including in the procedure
digestion with lesion-specific enzymes such as formamidopyrimidine DNA glycosylase
(which converts oxidised purines to breaks) or endonuclease III (recognising
oxidised pyrimidines). We summarise reports in which these enzymes have been used
to study a variety of nanomaterials in diverse cell types. We also stress that it
is important to carry out tests of cell viability alongside the genotoxicity assay,
since cytotoxicity can lead to adventitious DNA damage. Different concentrations of
nanomaterials should be investigated, concentrating on a non-cytotoxic range; and
incubating for short and longer periods can give valuable information about the
mode of damage induction. The use of lesion-specific enzymes can substantially
enhance the sensitivity of the comet assay in detecting genotoxic effects.
AN - rayyan-553780332
AU - Collins, A.
AU - El Yamani, N.
AU - Dusinska, M.
DO - 10.1016/j.freeradbiomed.2017.02.001
KW - DNA (Cytosine-5-)-Methyltransferase
DNA, B-Form
DNA, A-Form
DNA-(Apurinic or Apyrimidinic Site) Lyase
DNA
PY - 2017
SN - 0891-5849 1873-4596
SP - 69-76
ST - Sensitive detection of DNA oxidation damage induced by nanomaterials
T2 - FREE RADICAL BIOLOGY AND MEDICINE
TI - Sensitive detection of DNA oxidation damage induced by nanomaterials
VL - 107
Y2 - 6
ID - 8364
ER -
TY - JOUR
AB - Conventional treatment of inflammatory bowel disease (IBD) is based on the
daily administration of high doses of immune-suppressant or anti-inflammatory
drugs, often complicated by serious adverse effects. Thus, a carrier system that
delivers the drug specifically to the inflamed intestinal regions and shows
prolonged drug release would be desirable. The advent of TNF-α antibodies and other
biopharmaceuticals as potent and specific immune modulators in recent years has
broadened the treatment options in IBD, but further increases the necessity for
adequate drug delivery, as integrity and bioactivity of the biological active have
to be ensured. Exploiting the pathophysiological idiosyncrasies of IBD such as
increased mucus production, changes in the structure of the intestinal epithelium
and invasion of activated macrophages, different colloidal drug carrier systems
have been designed to passively or actively target the site of inflammation. This
review introduces different micro- or nanoparticulate drug delivery systems for
oral application in IBD therapy for the delivery of small molecular compounds and
next generation therapeutics from the group of biological (i.e. peptide and
nucleotide based) drugs. Crown Copyright © 2012 Published by Elsevier B.V. All
rights reserved.
AN - rayyan-553780334
AU - Collnot, E. M.
AU - Ali, H.
AU - Lehr, C. M.
DO - 10.1016/j.jconrel.2012.01.028
IS - 2
KW - Crohn's disease
Nanomedicine
Nanoparticle
Oral
Ulcerative colitis
Animals
Biological Agents
Drug Carriers
Humans
Inflammatory Bowel Diseases
Intestinal Mucosa
Models, Animal
Nanoparticles
Nucleotides
Polymers
Diseases
Drug delivery
Drug products
Medical nanotechnology
adalimumab
alicaforsen
budesonide
curcumin
cyclosporin
cyclosporin A
dexamethasone
drug carrier
eudragit
gelatin
glucocorticoid
infliximab
interleukin 10
Lactobacillus casei extract
liposome
mercaptopurine
mesalazine
methotrexate
microsphere
nanocarrier
nanocrystal
nanoparticle
olsalazine
rolipram
salazosulfapyridine
selenium
silver
tacrolimus
Activated macrophages
Adverse effect
Biopharmaceuticals
Carrier systems
Colloidal drug carriers
Conventional treatments
Drug carrier
Drug release
High dose
Immune modulators
Inflammatory bowel disease
Intestinal epithelium
Intestinal mucosa
Molecular compounds
Nanoparticulate drug delivery systems
Pathophysiological
bone marrow toxicity
chemical bond
Crohn disease
Cushing syndrome
dendritic cell
diabetes mellitus
diarrhea
disease model
disease severity
drug absorption
drug conjugation
drug contraindication
drug efficacy
drug formulation
drug stability
embryotoxicity
enteritis
enzyme degradation
experimental animal
gene expression
helper cell
hematologic disease
human
hydrophilicity
hypertension
immune deficiency
infection
intestine motility
intestine mucosa
kidney disease
lipid peroxidation
liver toxicity
lymphoma
macrophage
mast cell
membrane permeability
meta analysis (topic)
microemulsion
natural killer cell
nephrotoxicity
neutrophil
nonhuman
opportunistic infection
osteoporosis
oxidation
pancreatitis
paresthesia
particle size
priority journal
review
RNA interference
tremor
ulcerative colitis
Drug dosage
PY - 2012
SP - 235-246
ST - Nano- and microparticulate drug carriers for targeting of the inflamed
intestinal mucosa
T2 - Journal of Controlled Release
TI - Nano- and microparticulate drug carriers for targeting of the inflamed
intestinal mucosa
84862644846&doi=10.1016%2fj.jconrel.2012.01.028&partnerID=40&md5=83427e10f92305efc9
ba9ed22872a8d4
VL - 161
ID - 8366
ER -
TY - JOUR
AB - Metallic nanomaterials, including silver, gold, and iron oxide, are being
utilized in an increasing number of fields and specialties. The use of nanosilver
as an antimicrobial agent is becoming ever-more common, whereas gold and Iron oxide
nanomaterials are frequently utilized in the medical field due to their recognized
"biocompatibility". Numerous reports have examined the general toxicity of these
nanomaterials; however, little data exists on how the introduction of these
nanomaterials, at nontoxic levels, affects normal cellular processes. In the
present study the impact of low levels of 10 nm silver (Ag-NP), gold (Au-NP), and
iron oxide nanoparticles (SPION) on epidermal growth factor (EGF) signal
transduction within the human epithelial cell line, A-431, was Investigated.
Following a biocompatibility assessment, the nanoparticle-induced interference at
four specific targets within the EGF signaling process was evaluated: (1)
nanoparticle-EGF association, (2) Akt and Erk phosphorylation, (3) Akt activity,
and (4) EGF-dependent gene regulation. For all tested nanoparticles, following
cellular exposure, a disruption in the EGF signaling response transpired; however,
the metallic composition determined the mechanism of alteration. In addition to
inducing high quantities of ROS, Ag-NPs attenuated levels of Akt and Erk
phosphorylation. Au-NPs were found to decrease EGF-dependent Akt and Erk
phosphorylation as well as inhibit Akt activity. Lastly, SPIONs produced a strong
alteration In EGF activated gene transcription, with targeted genes influencing
cell proliferation, migration, and receptor expression. These results demonstrate
that even at low doses, Introduction of Ag-NPs, Au-NPs, and SPIONs impaired the A-
431 cell line's response to EGF.
AN - rayyan-553780336
AU - Comfort, K. K.
AU - Maurer, E. I.
AU - Braydich-Stolle, L. K.
AU - Hussain, S. M.
DO - 10.1021/nn203785a
IS - 12
KW - Epidermal Growth Factor
Signal Transduction
PY - 2011
SN - 1936-0851 1936-086X
SP - 10000-10008
ST - Interference of Silver, Gold, and Iron Oxide Nanoparticles on Epidermal
Growth Factor Signal Transduction in Epithelial Cells
T2 - ACS NANO
TI - Interference of Silver, Gold, and Iron Oxide Nanoparticles on Epidermal
Growth Factor Signal Transduction in Epithelial Cells
VL - 5
Y2 - 12
ID - 8368
ER -
TY - JOUR
AB - Local delivery of drugs or antimicrobial agents is a suitable approach in the
management of periodontitis when the infection is localized deep in the pockets and
does not adequately respond to mechanical debridement and/or systemic antibiotic
treatment. In this context, the objective of this study was to prepare new
biocomposite films with antimicrobial, anti-inflammatory, and good mechanical
properties to be applied in periodontal pockets. The composite film is eco-friendly
synthesized from poly(vinyl alcohol) (PVA) cross-linked with oxidized chitosan
(OxCS). Silver nanoparticles (AgNps) were inserted during film synthesis by adding
freshly chitosan-capped AgNps colloidal solution to the polymer mixture; the
addition of AgNps up to 1.44 wt.% improves the physico-chemical properties of the
film. The characterization of the films was performed by FT-IR, atomic mass
spectrometry, X-ray spectroscopy, and SEM. The films displayed a high swelling
ratio (162%), suitable strength (1.46 MPa), and excellent mucoadhesive properties
(0.6 N). Then, ibuprofen (IBF) was incorporated within the best film formulation,
and the IBF-loaded PVA/OxCS-Ag films could deliver the drug in a sustained manner
up to 72 h. The biocomposite films have good antimicrobial properties against
representative pathogens for oral cavities. Moreover, the films are biocompatible,
as demonstrated by in vitro tests on HDFa cell lines. © 2022 by the authors.
AN - rayyan-553780337
AU - Constantin, M.
AU - Lupei, M.
AU - Bucatariu, S. M.
AU - Pelin, I. M.
AU - Doroftei, F.
AU - Ichim, D. L.
AU - Daraba, O. M.
AU - Fundueanu, G.
DO - 10.3390/polym15010004
IS - 1
KW - drug delivery systems
ibuprofen
periodontitis
poly(vinyl alcohol)/chitosan nanocomposite films
Biocompatibility
Chitosan
Crosslinking
Diseases
Mass spectrometry
Metal nanoparticles
Microorganisms
Nanocomposite films
Polyvinyl alcohols
Sols
Thin films
Biocomposite films
Drug-delivery systems
Ibuprofen
Oxidized chitosan
Periodontiti
Poly (vinyl alcohol) (PVA)
Poly(vinyl alcohol)
Poly(vinyl alcohol) (PVA)
Poly(vinyl alcohol)/chitosan nanocomposite film
Thin-films
Periodontal Diseases
PY - 2023
ST - PVA/Chitosan Thin Films Containing Silver Nanoparticles and Ibuprofen for the
Treatment of Periodontal Disease
T2 - Polymers
TI - PVA/Chitosan Thin Films Containing Silver Nanoparticles and Ibuprofen for the
Treatment of Periodontal Disease
85146055699&doi=10.3390%2fpolym15010004&partnerID=40&md5=034794f9e2c2dab7e327d0337c
362f54
VL - 15
ID - 8369
ER -
TY - JOUR
AB - Alterations of skin homeostasis are widely diffused in our everyday life both
due to accidental injuries, such as wounds and burns, and physiological conditions,
such as late-stage diabetes, dermatitis, or psoriasis. These events are locally
characterized by an intense inflammatory response, a high generation of harmful
free radicals, or an impairment in the immune response regulation, which can
profoundly change the skin tissue’ repair process, vulnerability, and
functionality. Moreover, diabetes diffusion, antibiotic resistance, and abuse of
aggressive soaps and disinfectants following the COVID-19 emergency could be causes
for the future spreading of skin disorders. In the last years, hydroxycinnamic
acids and derivatives have been investigated and applied in several research fields
for their anti-oxidant, anti-inflammatory, and anti-bacterial activities. First, in
this study, we give an overview of these natural molecules’ current source and
applications. Afterwards, we review their potential role as valid alternatives to
the current therapies, supporting the management and rebalancing of skin disorders
and diseases at different levels. Also, we will introduce the recent advances in
the design of biomaterials loaded with these phenolic compounds, specifically
suitable for skin disorders treatments. Lastly, we will suggest future perspectives
for introducing hydroxycinnamic acids and derivatives in treating skin disorders. ©
2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780338
AU - Contardi, M.
AU - Lenzuni, M.
AU - Fiorentini, F.
AU - Summa, M.
AU - Bertorelli, R.
AU - Suarato, G.
AU - Athanassiou, A.
IS - 7
KW - Anti-bacterial
Anti-inflammatory
Anti-oxidants
Biomaterials
Hydroxycinnamic acids
Skin care
Skin disorders
1 chloro 2,4 dinitrobenzene
anthocyanin
caffeic acid
chitosan
chlorogenic acid
cinnamic acid
corticosteroid
coumaric acid
essential oil
ferulic acid
flavonoid
gallic acid
hyaluronic acid
hydroxyproline
inflammasome
interleukin 13
interleukin 17
limonene
malonaldehyde
methotrexate
microsphere
molecular scaffold
monophenol monooxygenase
n,n dimethylformamide
nanocomposite
nitric oxide
phosphodiesterase IV inhibitor
plant extract
polyvinyl alcohol
quercetin
rosmarinic acid
silver nanoparticle
syringic acid
transforming growth factor beta
uric acid
vitamin D
Article
atopic dermatitis
biocompatibility
biodegradability
bioremediation
contact dermatitis
crystallization
DNA damage
drug formulation
electrochemistry
electrospinning
emotional stress
emulsion
epithelization
flow kinetics
gene therapy
genetic engineering
granulation tissue
human
hyperkeratosis
hyperpigmentation
immune response
inflammation
keratinocyte
Leishmania braziliensis
leishmaniasis
lipid peroxidation
melanogenesis
microalga
microbial community
microemulsion
oxidative stress
paw edema
phototherapy
prevalence
pruritus
psoriasis
rosemary
Saccharomyces cerevisiae
shear stress
signal transduction
skin defect
skin disease
skin irritation
skin permeability
skinfold thickness
thermogravimetry
thermostability
three dimensional printing
tissue regeneration
wound healing
Skin
PY - 2021
ST - Hydroxycinnamic acids and derivatives formulations for skin damages and
disorders: A review
T2 - Pharmaceutics
TI - Hydroxycinnamic acids and derivatives formulations for skin damages and
disorders: A review
85110041221&doi=10.3390%2fpharmaceutics13070999&partnerID=40&md5=6ae54ab54575db7229
9785acc75d907e
VL - 13
ID - 8370
ER -
TY - JOUR
AB - Due to the increasing interest in nanotechnology in very large application
fields, including biotechnology, electronics and food industries, humans are
increasingly exposed to nanoparticles (NPs). Consequently, the question about the
safety of these nanomaterials and their impact on human health is a legitimate
concern. The liver is the primary organ of detoxification and is one of the tissues
that is most exposed to NPs. When they reach the bloodstream, NPs are mainly
internalized by liver cells. This review focuses on recent in vitro and in vivo
studies addressing the effects of organic and inorganic NPs on the liver.
Specifically, the impact of the NPs on hepatic enzyme activities, the inflammatory
response and genotoxicity processes will be described. Depending on the
physicochemical parameters of the NPs and the conditions of exposure, NPs could
lead to global liver injury.
AN - rayyan-553780339
AU - Cornu, R.
AU - Beduneau, A.
AU - Martin, H.
DO - 10.1016/j.tox.2019.152344
KW - Liver
PY - 2020
SN - 0300-483X
ST - Influence of nanoparticles on liver tissue and hepatic functions: A review
T2 - TOXICOLOGY
TI - Influence of nanoparticles on liver tissue and hepatic functions: A review
VL - 430
Y2 - 1
ID - 8371
ER -
TY - JOUR
AB - The combination of sulfadiazine and pyrimethamine plus folinic acid is the
conventional treatment for congenital toxoplasmosis. However, this classical
treatment presents teratogenic effects and bone marrow suppression. In this sense,
new therapeutic strategies are necessary to reduce these effects and improve the
control of infection. In this context, biogenic silver nanoparticles (AgNp-Bio)
appear as a promising alternative since they have antimicrobial, antiviral, and
antiparasitic activity. The purpose of this study to investigate the action of
AgNp-Bio in BeWo cells, HTR-8/SVneo cells and villous explants and its effects
against Toxoplasma gondii infection. Both cells and villous explants were treated
with different concentrations of AgNp-Bio or combination of sulfadiazine +
pyrimethamine (SDZ + PYZ) in order to verify the viability. After, cells and villi
were infected and treated with AgNp-Bio or SDZ + PYZ in different concentrations to
ascertain the parasite proliferation and cytokine production profile. AgNp-Bio
treatment did not reduce the cell viability and villous explants. Significant
reduction was observed in parasite replication in both cells and villous explants
treated with silver nanoparticles and classical treatment. The AgNp-Bio treatment
increased of IL-4 and IL-10 by BeWo cells, while HTR8/SVneo cells produced
macrophage migration inhibitory factor (MIF) and IL-4. In the presence of T.
gondii, the treatment induced high levels of MIF production by BeWo cells and IL-6
by HTR8SV/neo. In villous explants, the AgNp-Bio treatment downregulated production
of IL-4, IL-6, and IL-8 after infection. In conclusion, AgNp-Bio can decrease T.
gondii infection in trophoblast cells and villous explants. Therefore, this
treatment demonstrated the ability to reduce the T. gondii proliferation with
induction of inflammatory mediators in the cells and independent of mediators in
chorionic villus which we consider the use of AgNp-Bio promising in the treatment
of toxoplasmosis in BeWo and HTR8/SVneo cell models and in chorionic villi. ©
Copyright © 2021 Costa, Ribeiro, Silva Franco, da Silva, de Araújo, Milián, Luz,
Guirelli, Nakazato, Mineo, Mineo, Barbosa and Ferro.
AN - rayyan-553780340
AU - Costa, I. N.
AU - Ribeiro, M.
AU - Silva Franco, P.
AU - da Silva, R. J.
AU - de Araújo, T. E.
AU - Milián, I. C. B.
AU - Luz, L. C.
AU - Guirelli, P. M.
AU - Nakazato, G.
AU - Mineo, J. R.
AU - Mineo, T. W. P.
AU - Barbosa, B. F.
AU - Ferro, E. A. V.
DO - 10.3389/fmicb.2020.623947
KW - congenital toxoplasmosis
nanoparticles treatment
placenta
Toxoplasma gondii
trophoblast
antiparasitic agent
biogenic silver nanoparticle
deoxycholate sodium
folinic acid
formazan
interleukin 10
interleukin 2
interleukin 4
interleukin 6
interleukin 8
macrolide
macrophage migration inhibition factor
migration inhibition factor
mitogen activated protein kinase 3
nanoparticle
nitric oxide
pyrimethamine
silver nanoparticle
spiramycin
sulfadiazine
unclassified drug
anemia
antiparasitic activity
Article
BeWo cell line
blood vessel reactivity
CD4+ T lymphocyte
cell proliferation
cell viability
cesarean section
chorion villus
controlled study
cytokine production
cytotoxicity
cytotoxicity assay
cytotrophoblast
drug combination
enzyme activity
explant
fetus membrane
filamentous fungus
fungal biomass
gene expression
granulocytopenia
hippocampus
human
human cell
hypertension
immune response
Leishmania
limit of detection
macrophage
macrophage migration
megaloblastic anemia
MTT assay
nonhuman
parasite
parasitism
preeclampsia
protein expression
stillbirth
syncytiotrophoblast
tachyzoite
toxoplasmosis
Trypanosoma cruzi
Humanities
Humanism
Humans
PY - 2021
ST - Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both
Human Trophoblast Cells and Villous Explants
T2 - Frontiers in Microbiology
TI - Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both
Human Trophoblast Cells and Villous Explants
85100542403&doi=10.3389%2ffmicb.2020.623947&partnerID=40&md5=7e2909d8d5c68ef5590fc0
be9415ed4c
VL - 11
ID - 8372
ER -
TY - JOUR
AB - Electroactive materials can be taken to advantage for the development of
sensors and actuators as well as for novel tissue engineering strategies.
Composites based on poly(vinylidene fluoride), PVDF, have been evaluated with
respect to their biological response. Cell viability and proliferation were
performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts
and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-week-old
C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible
materials promoting cell response and not showing in vivo pro-inflammatory effects
which renders both of them attractive for biological applications and tissue
engineering, opening interesting perspectives to development of scaffolds from
these composites. Ferrite and silver nanoparticle composites decrease osteoblast
cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon
nanotube composites result in a significant increase in local vascularization
accompanied an increase of inflammatory markers after implantation.
AN - rayyan-553782076
AU - Costa, R.
AU - Ribeiro, C.
AU - Lopes, A. C.
AU - Martins, P.
AU - Sencadas, V.
AU - Soares, R.
AU - Lanceros-Mendez, S.
DO - 10.1007/s10856-012-4808-y
IS - 2
J2 - J Mater Sci Mater Med
KW - Animals
Cell Adhesion/drug effects
Cell Differentiation/drug effects
Cells, Cultured
Fibroblasts/*drug effects/physiology
Humans
Materials Testing
Mesenchymal Stem Cells/drug effects/physiology
Mice
Mice, Inbred C57BL
Osteoblasts/*drug effects/physiology
Polymers/chemical synthesis/chemistry/*pharmacology
Polyvinyls/*chemistry/pharmacology
Tissue Engineering/instrumentation/methods
Tissue Scaffolds/chemistry
RNA, Messenger
Polyglactin 910
Poly A-U
Poly I-C
Osteoblasts
Fibroblasts
Fluorides
LA - eng
N1 - Department of Biochemistry (U38-FCT), Faculty of Medicine, University of
Porto, 4200-319, Porto, Portugal.
PY - 2013
SP - 395-403
ST - Osteoblast, fibroblast and in vivo biological response to poly(vinylidene
fluoride) based composite materials
T2 - Journal of materials science. Materials in medicine
TI - Osteoblast, fibroblast and in vivo biological response to poly(vinylidene
fluoride) based composite materials
VL - 24
Y2 - 2
ID - 9989
ER -
TY - JOUR
AB - Amaranthus spp. são plantas nefrotóxicas popularmente conhecidas como
"caruru". Em casos de intoxicação por estas plantas, a principal alteração
histopatológica está presente no rim, sob forma de nefrose tubular tóxica, porém em
alguns casos pode haver alterações cardíacas. Alterações no eletrocardiograma,
compatíveis com quadros de hipercalemia, foram descritas em suínos intoxicados por
Amaranthus retroflexus e lesões como degeneração e necrose de miócitos cardíacos
descritas em suínos intoxicados por A. caudatus e ovinos intoxicados por A.
spinosus. Há dúvidas com relação às alterações cardíacas, que, na maioria dos
casos, são incipientes, o que pode levar a erros de interpretação. Para a
realização do trabalho foram utilizados blocos parafinados oriundos de um surto
natural de intoxicação por A. spinosus no sudeste do Brasil. Esse estudo teve como
objetivo detectar a presença de alterações regressivas incipientes no miocárdio de
ovinos intoxicados por A. spinosus, através da utilização imuno-histoquímica do
anticorpo anti-troponina C. Foram utilizados fragmentos de coração de 8 ovinos
adultos e 2 fetos, intoxicados naturalmente por A. spinosus. Estes fragmentos foram
submetidos à técnica de imuno-histoquímica com a utilização do anticorpo anti-
troponina C. Pela avaliação imuno-histoquímica do coração dos oito ovinos adultos
observaram-se diversos grupos de miócitos com diminuição significativa ou ausência
de imunorreatividade para o anticorpo anti-troponina C; essas áreas correspondiam,
em grande parte, aos mesmos grupos de miócitos que apresentavam, pela coloração de
Hematoxilina e Eosina (H.E.) alterações que variavam de leve tumefação celular a
aumento da eosinofilia, perda de estriação, lise celular e cariólise, ou mais
raramente, acompanhadas de infiltrado inflamatório... Amaranthus spp. are
nephrotoxic plants popularly known as "pigweed". In cases of poisoning by these
plants, the main histopathological alteration is found in the kidneys as toxic
tubular nephrosis; however, in some cases, there may be cardiac changes. ECG
changes associated with hyperkalemia have been described in pigs poisoned by
Amaranthus retroflexus. Degeneration and necrosis of myocytes have been described
in pigs poisoned by A. caudatus and sheep poisoned by A. spinosus. There are doubts
regarding cardiac changes, since in most cases they are incipient and don't exhibit
inflammatory reaction, which can lead to misinterpretation. For this study,
paraffin blocks with tissues from a poisoning outbreak by A. spinosus in
southeastern Brazil were used. The objective of the study was to detect the
presence of incipient regressive changes in the myocardium of sheep poisoned by A.
spinosus using anti-troponin C antibody-based immunohistochemistry. Fragments of
hearts from 8 adult sheep and 2 fetuses naturally poisoned by A. spinosus were
used. In the immunohistochemistry evaluation of the 8 hearts from the adult sheep
there were several groups of myocytes with significant decrease or absence of
immunoreactivity for anti-troponin C antibody. In most cases, these same areas on
Hematoxylin and Eosin (HE) staining exhibited changes that varied from mild
cellular tumefaction to increased eosinophilia, as well as loss of striation, cell
lysis and karyolysis, sometimes accompanied by inflammatory infiltrate...
AN - rayyan-553780341
AU - Costa, Samay Z. R.
AU - Peixoto, Paulo V.
AU - Brust, Luiz Armando C.
AU - D'Avila, Mariana S.
AU - Santos, André M.
AU - Driemeier, David
AU - Nogueira, Vivian de A.
AU - França, Ticiana N.
DO - 10.1590/S0100-736X2016000200004
IS - 2
KW - Amaranthaceae
Amaranthus spinosus
Caruru
Imuno-histoquímica
Insuficiência renal
Intoxicação por plantas
Miocárdio
Ovinos
Pigweed
Plant poisoning
Plantas tóxicas
Poisonous plants
Renal failure
Sheep, myocardium
Troponin C
Troponina C
LA - pt
PY - 2016
SN - 0100-736X
SP - 83-89
ST - Troponina C na detecção imuno-histoquímica de alterações regressivas precoces
no miocárdio de ovinos naturalmente intoxicados por Amaranthus spinosus
(Amaranthaceae)
T2 - Pesqui. vet. bras
TI - Troponina C na detecção imuno-histoquímica de alterações regressivas precoces
no miocárdio de ovinos naturalmente intoxicados por Amaranthus spinosus
(Amaranthaceae)
736X2016000200083
VL - 36
Y2 - 2 y3 - 1
ID - 8373
ER -
TY - JOUR
AB - Biodegradability and mechanical properties of magnesium alloys are attractive
for orthopaedic and cardiovascular applications. In order to study their
cytotoxicity usually bone cells are used. However, after implantation, diverse and
versatile cells are recruited and interact. Among the first ones coming into play
are cells of the immune system, which are responsible for the inflammatory
reaction. Macrophages play a central role in the inflammatory process due to the
production of cytokines involved in the tissue healing but also in the possible
failure of the implants. In order to evaluate the in vitro influence of the
degradation products of magnesium-based alloys on cytokine release, the extracts of
pure magnesium and two magnesium alloys (with gadolinium and silver as alloying
elements) were examined in an inflammatory in vitro model. Human promonocytic cells
(U937 cells) were differentiated into macrophages and further cultured with
magnesium-based extracts for 1 and 3 days (simulating early and late inflammatory
reaction phases), either at 37 degrees C or at 39 degrees C (mimicking normal and
inflammatory conditions, respectively). All extracts exhibit very good
cytocompatibility on differentiated macrophages. Results suggest that M1 and even
more M2 profiles of macrophage were stimulated by the extracts of Mg. Furthermore,
Mg-10Gd and Mg-2Ag extracts introduced a nuancing effect by rather inhibiting
macrophage M1 profile. Magnesium based biomaterials could thus induce a faster
inflammation resolution while improving tissue repair. Statement of Significance
Macrophage are the key-cells during inflammation and can influence the fate of
tissue healing and implant performance. Magnesium-based implants are biodegradable
and bioactive. Here we selected an in vitro system to model early and late
inflammation and effect of pyrexia (37 degrees C versus 39 degrees C). We showed
the beneficial and nuancing effects of magnesium (Mg) and the selected alloying
elements (silver (Ag) and gadolinium (Gd)) on the macrophage polarisation. Mg
extracts exacerbated simultaneously the macrophage M1 and M2 profiles while Mg-2Ag
and Mg-10Gd rather inhibited the M1 differentiation. Furthermore, 39 degrees C
exhibited protective effect by either decreasing cytokine production or promoting
anti-inflammatory ones, with or without extracts. Mg-based biomaterials could thus
induce a faster inflammation resolution while improving tissue repair. (C) 2019
Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
AN - rayyan-553780343
AU - Costantino, M. D.
AU - Schuster, A.
AU - Helmholz, H.
AU - Meyer-Rachner, A.
AU - Willumeit-Romer, R.
AU - Luthringer-Feyerabend, B. J. C.
DO - 10.1016/j.actbio.2019.10.014
KW - Magnesium
PY - 2020
SN - 1742-7061 1878-7568
SP - 598-608
ST - Inflammatory response to magnesium-based biodegradable implant materials
T2 - ACTA BIOMATERIALIA
TI - Inflammatory response to magnesium-based biodegradable implant materials
VL - 101
Y2 - 1 y3 - 1
ID - 8375
ER -
TY - JOUR
AB - Biodegradability and mechanical properties of magnesium alloys are attractive
for orthopaedic and cardiovascular applications. In order to study their
cytotoxicity usually bone cells are used. However, after implantation, diverse and
versatile cells are recruited and interact. Among the first ones coming into play
are cells of the immune system, which are responsible for the inflammatory
reaction. Macrophages play a central role in the inflammatory process due to the
production of cytokines involved in the tissue healing but also in the possible
failure of the implants. In order to evaluate the in vitro influence of the
degradation products of magnesium-based alloys on cytokine release, the extracts of
pure magnesium and two magnesium alloys (with gadolinium and silver as alloying
elements) were examined in an inflammatory in vitro model. Human promonocytic cells
(U937 cells) were differentiated into macrophages and further cultured with
magnesium-based extracts for 1 and 3 days (simulating early and late inflammatory
reaction phases), either at 37 °C or at 39 °C (mimicking normal and inflammatory
conditions, respectively). All extracts exhibit very good cytocompatibility on
differentiated macrophages. Results suggest that M1 and even more M2 profiles of
macrophage were stimulated by the extracts of Mg. Furthermore, Mg-10Gd and Mg-2Ag
extracts introduced a nuancing effect by rather inhibiting macrophage M1 profile.
Magnesium-based biomaterials could thus induce a faster inflammation resolution
while improving tissue repair. STATEMENT OF SIGNIFICANCE: Macrophage are the key-
cells during inflammation and can influence the fate of tissue healing and implant
performance. Magnesium-based implants are biodegradable and bioactive. Here we
selected an in vitro system to model early and late inflammation and effect of
pyrexia (37 °C versus 39 °C). We showed the beneficial and nuancing effects of
magnesium (Mg) and the selected alloying elements (silver (Ag) and gadolinium (Gd))
on the macrophage polarisation. Mg extracts exacerbated simultaneously the
macrophage M1 and M2 profiles while Mg-2Ag and Mg-10Gd rather inhibited the M1
differentiation. Furthermore, 39 °C exhibited protective effect by either
decreasing cytokine production or promoting anti-inflammatory ones, with or without
extracts. Mg-based biomaterials could thus induce a faster inflammation resolution
while improving tissue repair.
AN - rayyan-553781888
AU - Costantino, M. D.
AU - Schuster, A.
AU - Helmholz, H.
AU - Meyer-Rachner, A.
AU - Willumeit-Römer, R.
AU - Luthringer-Feyerabend, B. J. C.
DO - 10.1016/j.actbio.2019.10.014
J2 - Acta Biomater
KW - Absorbable Implants/*adverse effects
Biocompatible Materials/*adverse effects
Cell Survival/drug effects
Gadolinium/analysis
Humans
Inflammation/*etiology/*pathology
Interleukin 1 Receptor Antagonist Protein/metabolism
Interleukin-1beta/metabolism
Magnesium/*adverse effects
Silver/analysis
U937 Cells
Magnesium
LA - eng
N1 - Department of Biological Characterisation, Division of Metallic Biomaterials,
Institute of Material Research, Helmholtz-Zentrum Geesthacht Centre for Materials
and Coastal research, Max-Planck-Strasse 1, 21502 Geesthacht, Germany.; Department
of Biological Characterisation, Division of Metallic Biomaterials, Institute of
Material Research, Helmholtz-Zentrum Geesthacht Centre for Materials and Coastal
research, Max-Planck-Strasse 1, 21502 Geesthacht, Germany.; Department of
Biological Characterisation, Division of Metallic Biomaterials, Institute of
research, Max-Planck-Strasse 1, 21502 Geesthacht, Germany. Electronic address:
berengere.luthringer@hzg.de.
PY - 2020
SP - 598-608
ST - Inflammatory response to magnesium-based biodegradable implant materials
T2 - Acta biomaterialia
TI - Inflammatory response to magnesium-based biodegradable implant materials
VL - 101
Y2 - 1 y3 - 1
ID - 9812
ER -
TY - JOUR
AB - Current advances in enzyme bioscavenger prophylactic therapy against chemical
warfare nerve agent (CWNA) exposure are moving towards the identification of
catalytic bioscavengers that can degrade large doses of organophosphate (OP) nerve
agents without self destruction. This is a preferred method compared to therapy
with the purified stoichiometric bioscavenger, butyrylcholinesterase, which binds
OPs 1:1 and would thus require larger doses for treatment. Paraoxonase-1 (PON-1) is
one such catalytic bioscavenger that has been shown to hydrolyze OP insecticides
and contribute to detoxification in animals and humans. Here we investigated the
effects of a common red wine ingredient, Resveratrol (RSV), to induce the
expression of PON-1 in the human hepatic cell line HC04 and evaluated the
protection against CWNA simulants. Dose-response curves showed that a concentration
of 20 microM RSV was optimal in inducing PON-1 expression in HC04 cells. RSV at 20
microM increased the extracellular PON-1 activity approximately 150% without
significantly affecting the cells. Higher doses of RSV were cytotoxic to the cells.
Resveratrol also induced PON-1 in the human lung cell line A549. RSV pre-treatment
significantly (P = 0.05) protected the hepatic cells against exposure to 2x LD(50)
of soman and sarin simulants. However, lung cells were protected against soman
simulant exposure but not against sarin simulant exposure following RSV treatment.
In conclusion, these studies indicate that dietary inducers, such as RSV, can up-
regulate PON-1, a catalytic bioscavenger, which can then hydrolyze and protect
against CWNA-induced toxicity, providing a prospective new method to protect
against CWNA exposure.
AN - rayyan-553782096
AU - Curtin, B. F.
AU - Seetharam, K. I.
AU - Dhoieam, P.
AU - Gordon, R. K.
AU - Doctor, B. P.
AU - Nambiar, M. P.
DO - 10.1002/jcb.21543
IS - 5
J2 - J Cell Biochem
KW - Animals
Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
Aryldialkylphosphatase/*metabolism
Butyrylcholinesterase/metabolism
Cell Line
Chemical Warfare Agents/*toxicity
Enzyme Induction/drug effects
Humans
Mice
Resveratrol
Sarin/*toxicity
Soman/*toxicity
Stilbenes/*pharmacology
Humanities
Humanism
LA - eng
N1 - Department of Biochemical Pharmacology, Division of Biochemistry, Walter Reed
Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, Maryland 20910-
7500, USA.
PY - 2008
SP - 1524-35
ST - Resveratrol induces catalytic bioscavenger paraoxonase 1 expression and
protects against chemical warfare nerve agent toxicity in human cell lines
T2 - Journal of cellular biochemistry
TI - Resveratrol induces catalytic bioscavenger paraoxonase 1 expression and
protects against chemical warfare nerve agent toxicity in human cell lines
VL - 103
Y2 - 4 y3 - 1
ID - 10007
ER -
TY - JOUR
AB - Nowadays Ag-points, gutta percha and amalgam are widely used as orthograde or
retrograde root-filling materials. The biocompatibility of these materials however
remains debatable. Ag-points are easy to manipulate and when used together with an
appropriate cement, offer an adequate seal. Yet an important disadvantage is the
susceptibility to corrosion resulting in argyrosis and inflammatory reaction.
Tissue cultures proved these corrosion by products to be highly cytotoxic. Other
investigations, reported that the inflammatory tissue response seen with gutta
percha, may be due to small amounts of additives included in gutta percha. Equally,
there is no unanimity concerning the biocompatibility of amalgam, and the extent of
corrosion and inflammatory reaction is possibly related to the composition of the
amalgam used. Nevertheless in vivo studies demonstrated that, when freshly mixed
amalgam, gutta percha or Ag-points are implanted in the femur of rats, mature bone
was formed in direct contact with these materials.
AN - rayyan-553782247
AU - D'Haeseleire, P.
AU - Thielens, P.
AU - Bourgois, F.
AU - Vanclooster, R.
IS - 4
J2 - Acta Stomatol Belg
KW - Animals
Argyria/etiology
Corrosion
Dental Amalgam/*adverse effects
Gutta-Percha/*adverse effects
Humans
Rats
*Root Canal Obturation
Silver Compounds/*adverse effects
Aged
Aging
LA - dut
PY - 1989
SN - 0001-7000 (Print)
SP - 243-50
ST - [Are Ag-points, gutta percha and amalgam still applicable for apical
sealing?]
T2 - Acta stomatologica Belgica
TI - [Are Ag-points, gutta percha and amalgam still applicable for apical
sealing?]
VL - 86
Y2 - 12
ID - 10157
ER -
TY - JOUR
AB - Nowadays Ag-points, gutta percha and amalgam are widely used as orthograde or
retrograde root canal filling materials. The biocompatibility of these materials
however remains debatable. Ag-points are easy to manipulate and when used together
with an appropriate cement, offer an adequate seal. Yet an important disadvantage
is the susceptibility to corrosion resulting in argyrosis and inflammatory
reaction. Tissue cultures proved these corrosion by products to be highly
cytotoxic. Other investigations, reported that the inflammatory tissue response
seen with gutta percha, may be due to small amounts of additives included in gutta
percha. Equally, there is no unanimity concerning the biocompatibility of amalgam,
and the extent of corrosion and inflammatory reaction is possibly related to the
composition of the amalgam used. Nevertheless in vivo studies demonstrated that,
when freshly mixed amalgam, gutta percha or Ag-points are implanted in the femur of
rats, mature bone was formed in direct contact with these materials.
AN - rayyan-553782216
AU - D'Haeseleire, P.
AU - Thielens, P.
AU - Bourgois, F.
AU - Vanclooster, R.
IS - 4
J2 - Acta Stomatol Belg
KW - *Biocompatible Materials
Dental Amalgam/adverse effects
Gutta-Percha/adverse effects
Humans
Inflammation/chemically induced
Root Canal Filling Materials/*adverse effects
Silver/adverse effects
Aged
Aging
LA - dut
PY - 1989
SN - 0001-7000 (Print)
SP - 243-50
ST - [Are Ag-points, gutta percha and amalgam still useful as apical filling
materials?]
T2 - Acta stomatologica Belgica
TI - [Are Ag-points, gutta percha and amalgam still useful as apical filling
materials?]
VL - 86
Y2 - 12
ID - 10126
ER -
TY - JOUR
AB - Human triple-negative breast cancer (TNBC) being an aggressive cancer type
accounts for about 15-20% of global breast cancer cases. In the present study, the
cytotoxicity of pure silver (AgVI) and silver/zinc oxide (Ag/ZnOVI) nanostructures
was evaluated against the TNBC cells. The nanostructures synthesized from a green
route using Vateria indica (L.) fruit extract were characterized to scrutinize
their formation, crystal phase, size, shape, and surface properties via FTIR, PXRD,
FE-SEM coupled with EDS spectroscopy, and BET analysis. The results of the studies
have unveiled the formation of 26.43 nm and 20.97 nm sized AgVI and Ag/ZnOVI
nanostructures in their purest form. The in-vitro anticancer study performed on
human TNBC cells [MDA-MB468] revealed the enhancement in the antiproliferative
potentiality of bimetallic Ag/ZnOVI nanostructures from 66.99 ± 0.13 to
79.73 ± 0.23 in comparison to pure AgVI nanostructures. In addition to this, the
greenish yellow-fluorescence observed in the TNBC nuclei during the AO-EB staining
study manifested the early apoptosis. Furthermore, the anti-inflammatory and
cytotoxicity study performed on the human RBC and normal NIH3T3 murine fibroblasts
cells proved the biocompatibility and non-toxic nature of the synthesized
nanostructures with membrane stabilization percentage up to 94.5 ± 0.001.
Additionally, the antioxidant and antidiabetic studies carried out have
corroborated the radical scavenging and α-amylase inhibition capability up to
85.87 ± 0.001 and 89.60 ± 0.002 % respectively. Thus the overall results of the
study substantiate the superlative antioxidant, antidiabetic, and antiproliferative
property of green synthesized AgVI and Ag/ZnOVI nanostructures with excellent
biocompatibility.
AN - rayyan-553781927
AU - D'Souza, J. N.
AU - Nagaraja, G. K.
AU - Prabhu, A.
AU - Navada, K. M.
AU - Kouser, S.
AU - Manasa, D. J.
DO - 10.1016/j.ijpharm.2022.121450
J2 - Int J Pharm
KW - Animals
Anti-Inflammatory Agents/therapeutic use
Antioxidants/pharmacology/therapeutic use
*Dipterocarpaceae
Humans
Hypoglycemic Agents/therapeutic use
Mice
NIH 3T3 Cells
*Nanostructures
Plant Extracts/pharmacology
*Triple Negative Breast Neoplasms/drug therapy
Breast Neoplasms
Aged
Hypoglycemic Agents
LA - eng
N1 - Department of Chemistry, Mangalore University, Mangalagangothri-574199,
Karnataka, India.; Department of Chemistry, Mangalore University, Mangalagangothri-
574199, Karnataka, India. Electronic address: nagarajagk@gmail.com.; Yenepoya
Research Centre, Yenepoya (Deemed to be University), Deralakatte-575018, Karnataka,
India.; Department of Chemistry, Mangalore University, Mangalagangothri-574199,
Karnataka, India.; Department of Chemistry, Mangalore University, Mangalagangothri-
574199, Karnataka, India.; Department of Botany, Davanagere University, Davanagere-
577007, Karnataka, India.
PY - 2022
SP - 121450
ST - AgVI and Ag/ZnOVI nanostructures from Vateria indica (L.) exert antioxidant,
antidiabetic, anti-inflammatory and cytotoxic efficacy on triple negative breast
cancer cells in vitro
T2 - International journal of pharmaceutics
TI - AgVI and Ag/ZnOVI nanostructures from Vateria indica (L.) exert antioxidant,
antidiabetic, anti-inflammatory and cytotoxic efficacy on triple negative breast
cancer cells in vitro
VL - 615
Y2 - 3 y3 - 5
ID - 9848
ER -
TY - JOUR
AB - Background: Poly-lactic acid nanoparticles (PLA-NP) are a type of polymeric
NP, frequently used as nanomedicines, which have advantages over metallic NP such
as the ability to maintain therapeutic drug levels for sustained periods of time.
Despite PLA-NP being considered biocompatible, data concerning alterations in
cellular physiology are scarce. Methods: We conducted an extensive evaluation of
PLA-NP biocompatibility in human lung epithelial A549 cells using high throughput
screening and more complex methodologies. These included measurements of
cytotoxicity, cell viability, immunomodulatory potential, and effects upon the
cells' proteome. We used non-and green-fluorescent PLA-NP with 63 and 66 nm
diameters, respectively. Cells were exposed with concentrations of 2, 20, 100 and
200 mu g/mL, for 24, 48 and 72 h, in most experiments. Moreover, possible endocytic
mechanisms of internalization of PLA-NP were investigated, such as those involving
caveolae, lipid rafts, macropinocytosis and clathrin-coated pits. Results: Cell
viability and proliferation were not altered in response to PLA-NP. Multiplex
analysis of secreted mediators revealed a low-level reduction of IL-12p70 and
vascular epidermal growth factor (VEGF) in response to PLA-NP, while all other
mediators assessed were unaffected. However, changes to the cells' proteome were
observed in response to PLA-NP, and, additionally, the cellular stress marker
miR155 was found to reduce. In dual exposures of staurosporine (STS) with PLA-NP,
PLA-NP enhanced susceptibility to STS-induced cell death. Finally, PLA-NP were
rapidly internalized in association with clathrin-coated pits, and, to a lesser
extent, with lipid rafts. Conclusions: These data demonstrate that PLA-NP are
internalized and, in general, tolerated by A549 cells, with no cytotoxicity and no
secretion of pro-inflammatory mediators. However, PLA-NP exposure may induce
modification of biological functions of A549 cells, which should be considered when
designing drug delivery systems. Moreover, the pathways of PLA-NP internalization
we detected could contribute to the improvement of selective uptake strategies.
AN - rayyan-553780346
AU - da Luz, C. M.
AU - Boyles, M. S. P.
AU - Falagan-Lotsch, P.
AU - Pereira, M. R.
AU - Tutumi, H. R.
AU - Santos, E. D.
AU - Martins, N. B.
AU - Himly, M.
AU - Sommer, A.
AU - Foissner, I.
AU - Duschl, A.
AU - Granjeiro, J. M.
AU - Leite, P. E. C.
DO - 10.1186/s12951-016-0238-1
KW - Lactic Acid
RNA, Messenger
Poly I-C
Poly A-U
Poly G
Poly T
Polylysine
Polyglactin 910
PY - 2017
SN - 1477-3155
ST - Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications
in lung epithelial cells and are internalized by clathrin-coated pits and lipid
rafts
T2 - JOURNAL OF NANOBIOTECHNOLOGY
TI - Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications
in lung epithelial cells and are internalized by clathrin-coated pits and lipid
rafts
VL - 15
Y2 - 1 y3 - 31
ID - 8377
ER -
TY - JOUR
AB - This research aimed to assess the influence of dietary addition of rutin on
inflammation, apoptosis and antioxidative responses in muscle of silver catfish
(Rhamdia quelen) challenged with Aeromonas hydrophila (A. hydrophila). Fish were
split into four groups as follows: control, 0.15% rutin, A. hydrophila, 0.15% rutin
+ A. hydrophila. After 2 weeks of feeding with standard or rutin diets, fish were
challenged or not with A. hydrophila for 1 week. Rutin-added diet abrogates A.
hydrophila induced-hemorrhage and inflammatory infiltration. It decreases A.
hydrophila induced-apoptosis through decreasing the ratio of Bax to Bcl-2 and
increasing phospho-Akt to Akt ratio. It diminishes the A. hydrophila induced-rise
in nitric oxide and superoxide anion levels and re-establishes superoxide dismutase
activity as well. Although such diet is unable to recover the levels of reduced
glutathione (GSH), cysteine and glutamate cysteine ligase, which are depleted as a
result of A. hydrophila infection, it diminishes the oxidized glutathione (GSSG)
content, thus decreasing GSSG to GSH ratio. It increases the levels of cysteine
residues of proteins and diminishes those of thiol-protein mixed disulfides, which
were changed after A. hydrophila challenge. Finally, it reduces A. hydrophila
induced-lipid peroxidation, markedly elevates ascorbic acid and thus reestablishes
total antioxidant capacity, whose levels were decreased after A. hydrophila
challenge. In conclusion, the dietary addition of rutin at 0.15% impairs A.
hydrophila-induced inflammatory response, inhibits A. hydrophila-induced apoptosis
and promotes cell survival. It also reduces the A. hydrophila-induced oxidative
stress and stimulates the antioxidative responses in muscle of A. hydrophila-
infected silver catfish.
AN - rayyan-553780348
AU - da Rosa, V. M.
AU - Ariotti, K.
AU - Bressan, C. A.
AU - da Silva, E. G.
AU - Dallaporta, M.
AU - Junior, G. B.
AU - de Vargas, A. C.
AU - Finamor, I. A.
AU - Pavanato, M. A.
DO - 10.1016/j.cbpc.2019.108611
KW - Oxidative Stress
Apoptosis
PY - 2019
SN - 1532-0456 1878-1659
ST - Dietary addition of rutin impairs inflammatory response and protects muscle
of silver catfish (Rhamdia quelen) from apoptosis and oxidative stress in Aeromonas
hydrophila-induced infection
T2 - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
TI - Dietary addition of rutin impairs inflammatory response and protects muscle
VL - 226
Y2 - 12
ID - 8379
ER -
TY - JOUR
AB - This research aimed to assess the influence of dietary addition of rutin on
inflammation, apoptosis and antioxidative responses in muscle of silver catfish
(Rhamdia quelen) challenged with Aeromonas hydrophila (A. hydrophila). Fish were
split into four groups as follows: control, 0.15% rutin, A. hydrophila, 0.15% rutin
+ A. hydrophila. After 2 weeks of feeding with standard or rutin diets, fish were
challenged or not with A. hydrophila for 1 week. Rutin-added diet abrogates A.
hydrophila induced-hemorrhage and inflammatory infiltration. It decreases A.
hydrophila induced-apoptosis through decreasing the ratio of Bax to Bcl-2 and
increasing phospho-Akt to Akt ratio. It diminishes the A. hydrophila induced-rise
in nitric oxide and superoxide anion levels and reestablishes superoxide dismutase
activity as well. Although such diet is unable to recover the levels of reduced
glutathione (GSH), cysteine and glutamate cysteine ligase, which are depleted as a
result of A. hydrophila infection, it diminishes the oxidized glutathione (GSSG)
content, thus decreasing GSSG to GSH ratio. It increases the levels of cysteine
residues of proteins and diminishes those of thiol-protein mixed disulfides, which
were changed after A. hydrophila challenge. Finally, it reduces A. hydrophila
induced-lipid peroxidation, markedly elevates ascorbic acid and thus reestablishes
total antioxidant capacity, whose levels were decreased after A. hydrophila
challenge. In conclusion, the dietary addition of rutin at 0.15% impairs A.
hydrophila-induced inflammatory response, inhibits A. hydrophila-induced apoptosis
and promotes cell survival. It also reduces the A. hydrophila-induced oxidative
stress and stimulates the antioxidative responses in muscle of A. hydrophila-
infected silver catfish.
AN - rayyan-553782065
AU - da Rosa, V. M.
AU - Ariotti, K.
AU - Bressan, C. A.
AU - da Silva, E. G.
AU - Dallaporta, M.
AU - Júnior, G. B.
AU - de Vargas, A. C.
AU - Finamor, I. A.
DO - 10.1016/j.cbpc.2019.108611
KW - Aeromonas hydrophila
Animal Feed
Animals
Antioxidants/pharmacology
Apoptosis
Catfishes/*immunology
Dietary Supplements
Fish Diseases/*metabolism
*Gram-Negative Bacterial Infections/metabolism/veterinary
Muscles/*metabolism
Oxidative Stress
Protective Agents/pharmacology
Rutin/*pharmacology
LA - eng
Maria, RS, Brazil.; Department of Physiology and Pharmacology, Universidade Federal
de Santa Maria, RS, Brazil.; Department of Physiology and Pharmacology,
Universidade Federal de Santa Maria, RS, Brazil.; Department of Physiology and
Pharmacology, Universidade Federal de Santa Maria, RS, Brazil.; Department of
Morphology, Universidade Federal de Santa Maria, RS, Brazil.; Department of
Physiology and Pharmacology, Universidade Federal de Santa Maria, RS, Brazil.;
Department of Morphology, Universidade Federal de Santa Maria, RS, Brazil.;
Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria,
RS, Brazil.; Department of Physiology and Pharmacology, Universidade Federal de
Santa Maria, RS, Brazil.; Department of Physiology and Pharmacology, Universidade
Federal de Santa Maria, RS, Brazil. Electronic address: isabela.finamor@gmail.com.;
Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, RS,
Brazil. Electronic address: amaliapavanato@yahoo.com.br.
PY - 2019
SN - 1532-0456 (Print)
SP - 108611
ST - Dietary addition of rutin impairs inflammatory response and protects muscle
TI - Dietary addition of rutin impairs inflammatory response and protects muscle
VL - 226
Y2 - 12
ID - 9979
ER -
TY - JOUR
AB - A morphologic study of the lungs was carried out in Swiss mice infected with
yeast isolated from Paracoccidioides brasiliensis (Pbl8). The lung was processed 1,
2, 4, and 8 weeks after inoculation for histologic staining with hematoxylin and
eosin (H&E), methenamine silver nitrate (Gomori-Grocott), and picrosirius to
qualitative and quantitative analyses of the granulomas and the presence of fungal
lesions. The numbers of CFUs/g counted in the lungs were 189.8 ± 20.64, 353.6 ±
46.21, 547.2 ± 108.1, and 295.2 ±89.17 in the first, second, fourth, and eighth
weeks, respectively. One week after infection, inflammatory cells and reticular and
collagens fibers, the latest typical of fibrosis, were detected. After 2 and 4
weeks, a progressive intensification of the infection and fibrosis was observed,
but in week 8 a more orga-nized granuloma was evident, with macrophages,
epithelioid cells, and yeasts in the central portion, and intense peripheral
basophilia. Pycnotic structures typical of apoptotic bodies were observed in weeks
1 and 8. The different histologic stain-ing used acted as a fundamental tool for
the study of the morphologic organization of granuloma formation. Copyright © 2009
by The American Society of Tropical Medicine and Hygiene.
AN - rayyan-553780350
AU - Da Silva, F. C.
AU - Svidzinski, T. I. E.
AU - Patussi, E. V.
AU - Cardoso, C. P.
AU - De Oliveira Dalalio, M. M.
AU - Hernandes, L.
DO - 10.4269/ajtmh.2009.80.798
IS - 5
KW - Animals
Granuloma
Lung
Lung Diseases, Fungal
Male
Mice
Mice, Inbred BALB C
Paracoccidioides
Paracoccidioidomycosis
Mus
Paracoccidioides brasiliensis
collagen fiber
eosin
hematoxylin
silver nitrate
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
basophilia
colony forming unit
controlled study
disease course
disease duration
epithelioid cell
fungus isolation
histopathology
inflammatory cell
inoculation
lung
lung fibrosis
lung granuloma
macrophage
male
micromorphology
mouse
nonhuman
qualitative analysis
reticulum cell
South American blastomycosis
Swiss Webster mouse
yeast
animal
Bagg albino mouse
granuloma
lung mycosis
microbiology
pathology
PY - 2009
SP - 798-804
ST - Morphologic organization of pulmonary granulomas in mice infected with
T2 - American Journal of Tropical Medicine and Hygiene
TI - Morphologic organization of pulmonary granulomas in mice infected with
66149098517&doi=10.4269%2fajtmh.2009.80.798&partnerID=40&md5=956dd328be58b4acd34e6b
b3b201a1a2
VL - 80
ID - 8381
ER -
TY - JOUR
AB - In the present study, new-layered inorganic/organic hybrid of silver/talc
nanocomposites (Ag/Tlc-NPs) and its chitosan-capped derivative (Ag/Tlc/Csn NCs)
were biochemically synthesized utilizing Lawsonia inermis L. extract. The silver
nanoparticles (Ag NPs) were synthesized employing green method on the exterior
surface layer of talc mineral as a solid substrate. The negatively charged surface
layer of talc might function as templates and can attract the chitosan cations from
a solution to yield a layered hybrid structure, whose inorganic phase is formed by
Si-O-Ag bonds. Our results revealed that Ag NPs were formed on the exterior surface
of talc with a diameter with size of 124–215 nm. In addition, cytotoxicity, in
vitro antibacterial activity, and clinical effects of wound-healing ointments
containing talc were investigated. The results implied the successful synthesis of
Ag/Tlc/Csn NCs using the extract. The structures were safe up to 0.50 mg/mL. In
vitro studies confirmed antioxidant and antibacterial properties of Ag/Tlc/Csn NCs.
In sum, our findings showed that the ointments improve wound healing process by
inducing an anti-inflammatory M2 phenotype and bFGF, CD206, collagen1A, and IL-10
production that causes fibroblast migration and wound closure through influencing
M2 macrophage. Ag/Tlc/Csn is suggested to be taken into consideration as a medical
combination for improving infected wound healing and as a promising agent for
clinical administration. © 2021 Elsevier B.V.
AN - rayyan-553780351
AU - Daghian, S. G.
AU - Farahpour, M. R.
AU - Jafarirad, S.
DO - 10.1016/j.msec.2021.112294
KW - Chitosan
Collagen biosynthesis
Infected wound
Nanocomposites
Silver
Talc
Acceleration
Lawsonia Plant
Metal Nanoparticles
Static Electricity
Wound Healing
Bacteria
Cell culture
Silicon compounds
Silver compounds
antiinfective agent
chitosan
metal nanoparticle
nanocomposite
silver
talc
Ag$++$
Electrostatic attractions
Infected wounds
Inorganic-organic hybrid
Lawsonia inermis
Synthesised
Wound healing
acceleration
Lawsonia (plant)
static electricity
wound healing
Biochemistry
Staphylococcus
PY - 2021
ST - Biological fabrication and electrostatic attractions of new layered
silver/talc nanocomposite using Lawsonia inermis L. and its chitosan-capped
inorganic/organic hybrid: Investigation on acceleration of Staphylococcus aureus
and Pseudomonas aeruginosa infected wound healing
TI - Biological fabrication and electrostatic attractions of new layered
silver/talc nanocomposite using Lawsonia inermis L. and its chitosan-capped
inorganic/organic hybrid: Investigation on acceleration of Staphylococcus aureus
and Pseudomonas aeruginosa infected wound healing
85109214091&doi=10.1016%2fj.msec.2021.112294&partnerID=40&md5=0d096770290281577dfd6
f4eac572568
VL - 128
ID - 8382
ER -
TY - JOUR
AB - Copper is an essential metal for life, but is toxic at high concentrations.
In mammalian cells, two copper transporters are known, CTR1 and CTR2. In order to
gain insights on the possible influence of the import pathway on cellular responses
to copper, two copper challenges were compared: one with copper ion, which is
likely to use preferentially CTR1, and one with a copper-polyacrylate complex,
which will be internalized via the endosomal pathway and is likely to use
preferentially CTR2. A model system consisting in the J774A1 mouse macrophage
system, with a strong endosomal/lysosomal pathway, was used. In order to gain wide
insights into the cellular responses to copper, a proteomic approach was used. The
proteomic results were validated by targeted experiments, and showed differential
effects of the import mode on cellular physiology parameters. While the
mitochondrial transmembrane potential was kept constant, a depletion in the free
glutahione content was observed with copper (ion and polylacrylate complex). Both
copper-polyacrylate and polyacrylate induced perturbations in the cytoskeleton and
in phagocytosis. Inflammatory responses were also differently altered by copper ion
and copper-polyacrylate. Copper-polyacrylate also perturbed several metabolic
enzymes. Lastly, enzymes were used as a test set to assess the predictive value of
proteomics. Significance: Proteomic profiling provides an in depth analysis of the
alterations induced on cells by copper under two different exposure modes to this
metal, namely as the free ion or as a complex with polyacrylate. The cellular
responses were substantially different between the two exposure modes, although
some cellular effects are shared, such as the depletion in free glutathione.
Targeted experiments were used to confirm the proteomic results. Some metabolic
enzymes showed altered activities after exposure to the copper-polyacrylate
complex. The basal inflammatory responses were different for copper ion and for the
copper-polyacrylate complex, while the two forms of copper inhibited
lipopolysaccharide-induced inflammatory responses.
AN - rayyan-553780355
AU - Dalzon, B.
AU - Devcic, J.
AU - Bons, J.
AU - Torres, A.
AU - Diemer, H.
AU - Ravanel, S.
AU - Cianferani, S.
AU - Carapito, C.
AU - Rabilloud, T.
DO - 10.1016/j.jprot.2021.104178
KW - Proteomics
Proteome
Macrophages
Copper
PY - 2021
SN - 1874-3919 1876-7737
ST - A proteomic view of cellular responses of macrophages to copper when added as
ion or as copper-polyacrylate complex
T2 - JOURNAL OF PROTEOMICS
TI - A proteomic view of cellular responses of macrophages to copper when added as
ion or as copper-polyacrylate complex
VL - 239
Y2 - 5 y3 - 15
ID - 8386
ER -
TY - JOUR
AB - Immunotoxicology sensu lato comprises not only toxicity toward immune cells,
but also biological reactions from immune cells exposed to toxicants, reactions
that may have deleterious effects at the organismal level. Within this wide frame,
a specific case of interest is represented by the response of macrophages to
particulate materials, with the epitome examples of asbestos and crystalline
silica. For such toxicants that are both persistent and often encountered in an
occupational setting, i.e. at low but repeated doses, there is a need for in vitro
systems that can take into account these two parameters. Currently, most in vitro
systems are used in an acute exposure mode, i.e., with a single dose and a readout
made shortly if not immediately after exposure. We describe here how adequate
changes of the culture methods applied to the murine macrophage cell line J774A.1
enable longer periods of culture (several days), which represents a first
opportunity to address the persistence and dose-rate issues. To respond to this,
the protocol uses a reduction in the concentration of the animal serum used for
cell culture, as well as a switch from fetal to adult serum, which is less rich in
proliferation factors. By doing so, we have considerably reduced cell
proliferation, which is a problem with cell lines when they are supposed to
represent slowly-dividing cells such as resident macrophages. We also succeeded in
maintaining the differentiated functions of macrophages, such as phagocytosis or
inflammatory responses, over the whole culture period. Furthermore, the presence of
serum, even at low concentrations, provides excellent cell viability and keeps the
presence of a protein corona on particulate materials, a feature that is known to
strongly modulate their effects on cells and is lost in serum-free culture. Besides
data showing the impact of these conditions on macrophages cell line cultures,
illustrative examples are shown on silica- and cobalt-based pigments.
AN - rayyan-553780356
AU - Dalzon, B.
AU - Torres, A.
AU - Devcic, J.
AU - Fenel, D.
AU - Sergent, J. A.
AU - Rabilloud, T.
DO - 10.3389/ftox.2021.780778
KW - Macrophages
PY - 2021
SN - 2673-3080
ST - A Low-Serum Culture System for Prolonged in Vitro Toxicology Experiments on a
Macrophage System
T2 - FRONTIERS IN TOXICOLOGY
TI - A Low-Serum Culture System for Prolonged in Vitro Toxicology Experiments on a
Macrophage System
VL - 3
Y2 - 12 y3 - 6
ID - 8387
ER -
TY - JOUR
AB - Introduction Leishmania braziliensis infection induces a large spectrum of
lesions that clinically manifest as nodules or papules that progress to ulcers.
Although it is already known that T helper cells predominate in the lesions,
cytotoxic T cells have also been reported to be present, and their role in
leishmaniasis immunopathogenesis is not well known. This study investigated the
amounts of CD8+ and granzyme B+ cells in different clinical forms of human
cutaneous leishmaniasis (CL). Methods Forty tissue fragments from early (E-CL) and
late CL (L-CL) lesions and from disseminated leishmaniasis (DL) - papules and
ulcers - were characterized. The inflamed area per fragment was calculated, and the
CD8 and granzyme B expression levels in the infiltrates were quantified by counting
positive cells in 15 fields. The localization of CD8 and granzyme B was graded
subjectively. Results Inflammation was higher in L-CL and DL ulcers. CD8 expression
was increased in late ulcerated lesions compared to recent lesions. The increase in
CD8+ cells also correlated with the duration of the lesion. Papules had a higher
frequency of granzyme B+ cells than E-CL lesions, although the frequency was
similar to those for late and DL ulcers. CD8+ cells were mostly found in the
papillary dermis. Conclusions CD8+ T and granzyme B+ cells are present in the
inflammatory infiltrates of CL and DL and may participate in the immunopathogenesis
of Leishmania infection. .
AN - rayyan-553780357
AU - Dantas, Marina Loyola
AU - Oliveira, Juliana Cabral de
AU - Carvalho, Lucas
AU - Passos, Sara Timoteo
AU - Queiroz, Adriano
AU - Machado, Paulo
AU - Carvalho, Edgar
AU - Arruda, Sergio
DO - 10.1590/S0037-86822013000600728
IS - 6
KW - CD8
Cutaneous
Cytotoxicity
Human
Inflammation
Leishmaniasis
Humanities
Humanism
Humans
LA - en
PY - 2013
SN - 0037-8682
SP - 728-734
ST - CD8+ T cells in situ in different clinical forms of human cutaneous
leishmaniasis
T2 - Rev. Soc. Bras. Med. Trop
TI - CD8+ T cells in situ in different clinical forms of human cutaneous
leishmaniasis
86822013000600728
VL - 46
Y2 - 12 y3 - 1
ID - 8388
ER -
TY - JOUR
AB - Polymeric nanoparticles (NPs) have the potential to provide effective and
safe delivery of antiretroviral drugs in the context of prophylactic anti-HIV
vaginal microbicides. Dapivirine-loaded poly(D,L-lactic-co-glycolic acid) (PLGA)
NPs were produced by an emulsion-solvent evaporation method, optimized for
colloidal properties using a 3-factor, 3-level Box-Behnken experimental design, and
characterized for drug loading, production yield, morphology, thermal behavior,
drug release, in vitro cellular uptake, cytotoxicity and pro-inflammatory
potential. Also, drug permeability/membrane retention in well-established HEC-1-A
and CaSki cell monolayer models as mediated by NPs was assessed in the absence or
presence of mucin. Box-Behnken design allowed optimizing monodisperse 170 nm drug-
loaded NPs. Drug release experiments showed an initial burst effect up to 4 h,
followed by sustained 24 h release at pH 4.2 and 7.4. NPs were readily taken up by
different genital and macrophage cell lines as assessed by fluorescence microscopy.
Drug-loaded NPs presented lower or at least similar cytotoxicity as compared to the
free drug, with up to around one-log increase in half-maximal cytotoxic
concentration values. In all cases, no relevant changes in cell pro-inflammatory
cytokine/chemokine production were observed. Dapivirine transport across cell
monolayers was significantly decreased when mucin was present at the donor side
with either NPs or the free drug, thus evidencing the influence of this natural
glycoprotein in membrane permeability. Moreover, drug retention in cell monolayers
was significantly higher for NPs in comparison with the free drug. Overall,
obtained dapivirine-loaded PLGA NPs possess interesting technological and
biological features that may contribute to their use as novel safe and effective
vaginal microbicides. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - rayyan-553780359
AU - das Neves, J.
AU - Sarmento, B.
DO - 10.1016/j.actbio.2015.02.007
KW - Anti-Infective Agents
PY - 2015
SN - 1742-7061 1878-7568
SP - 77-87
ST - Precise engineering of dapivirine-loaded nanoparticles for the development of
anti-HIV vaginal microbicides
TI - Precise engineering of dapivirine-loaded nanoparticles for the development of
anti-HIV vaginal microbicides
VL - 18
Y2 - 5
ID - 8390
ER -
TY - JOUR
AB - In this study, silver nanoparticles (PE-AgNPs) were synthesized using Premna
esculenta (PE) leaf extract, characterized by UV, TEM, TEM-EDX, XRD, DLS, and
ICPOES studies. AgNPs were found to be stable at -35 mV with 26.157 ppm
concentration. AgNPs were found triangular in shape with an average size of about
44 nm. Hepatoprotective activity was assessed in animal model using silymarin as
standard drug. Biochemical parameters (serum AST, ALT, gamma GT, ACP, and ALP),
inflammatory markers (IL 1 beta, IL 17, TNF alpha, and IL 10), and antioxidant
markers (GSH, SOD, Catalase, and LPO) were assayed. Liver tissue was processed for
histological analysis. Induction of hepatotoxicity increases AST, ALT, gamma GT,
ACP, ALP, IL 10, LPO, and decreases IL 1 beta, IL 17, TNF alpha, GSH, SOD, and
Catalase. PE-AgNPs treatment significantly decreases IL 10, LPO while IL 1 beta, IL
17, TNF alpha, GSH, and SOD were increased in animals. PE-AgNPs partially recovered
CCl4-induced changes in liver histology.
AN - rayyan-553780360
AU - Das, B. K.
AU - Ghosh, S.
AU - Gomes, A.
AU - De, U. C.
DO - 10.1080/24701556.2023.2181821
KW - Mice
PY - 2023
SN - 2470-1556 2470-1564
ST - Synthesis of silver nanoparticles using aqueous leaf extract of Premna
esculenta and in vivo evaluation of its hepatoprotective activity in Swiss albino
male mice
TI - Synthesis of silver nanoparticles using aqueous leaf extract of Premna
esculenta and in vivo evaluation of its hepatoprotective activity in Swiss albino
male mice
Y2 - 2 y3 - 18
ID - 8391
ER -
TY - JOUR
AB - Background The advances in products based on nanotechnology have directed
extensive research on low-cost, biologically compatible, and easily degradable
materials. Main body Sericin (SER) is a protein mainly composed of glycine, serine,
aspartic acid, and threonine amino acids removed from the silkworm cocoon
(particularly Bombyx mori and other species). SER is a biocompatible material with
economic viability, which can be easily functionalized due to its potential
crosslink reactions. Also, SER has inherent biological properties, which makes
possible its use as a component of pharmaceutical formulations with several
biomedical applications, such as anti-tumor, antimicrobials, antioxidants and as
scaffolds for tissue repair as well as participating in molecular mechanisms
attributed to the regulation of transcription factors, reduction of inflammatory
signaling molecules, stimulation of apoptosis, migration, and proliferation of
mesenchymal cells. Conclusion In this review, the recent innovations on SER-based
nano-medicines (nanoparticles, micelles, films, hydrogels, and their hybrid
systems) and their contributions for non-conventional therapies are discussed
considering different molecular mechanisms for promoting their therapeutic
applications.
AN - rayyan-553780361
AU - Das, G.
AU - Shin, H. S.
AU - Campos, E. V. R.
AU - Fraceto, L. F.
AU - Rodriguez-Torres, M. D.
AU - Mariano, K. C. F.
AU - de Araujo, D. R.
AU - Fernandez-Luqueno, F.
AU - Grillo, R.
AU - Patra, J. K.
DO - 10.1186/s12951-021-00774-y
IS - 1
KW - Sericins
PY - 2021
SN - 1477-3155
ST - Sericin based nanoformulations: a comprehensive review on molecular
mechanisms of interaction with organisms to biological applications
TI - Sericin based nanoformulations: a comprehensive review on molecular
mechanisms of interaction with organisms to biological applications
VL - 19
Y2 - 1 y3 - 22
ID - 8392
ER -
TY - JOUR
AB - Engineered nanoparticles (ENPs) offer technological advantages for a variety
of industrial and consumer products as well as show promise for biomedical
applications. Recent progress in the field of nanotechnology has led to increased
exposure to nanoparticles by humans. To date, little is known about the adverse
effects of these ENPs on reproductive health, although interest in nanotechnology
area is growing. A few biocompatible ENPs have a high loading capacity for
exogenous substances, including drugs, DNA or proteins, and can selectively deliver
molecular cargo into cells; however, they represent a potential tool for gene
delivery into gametes and embryos. Understanding the reprotoxicological aspects of
these ENPs is of the utmost importance to reliably estimate its potential impact on
human health. In addition, a search for protective agents to combat ENP-mediated
reproductive toxicity is warranted. Therefore, in this review we summarize the
toxic effects of a few ENPs (metal and metal oxides, carbon-based nanoparticles,
quantum dots and chitosan) in mammalian germ cells and developing embryos, and
propose some treatment strategies that could mitigate nanoparticle-mediated
toxicity. In addition, we outline the anticipated applications of ENPs in
transgenic animal production in order to generate models for investigations into
the mechanisms for human disease. A literature search was performed using the
National Center for Biotechnology Information PubMed database up until March 2016
and relevant keywords were used to obtain information regarding mammalian germ
cell-specific toxicity and embryotoxicity of ENPs, possible treatment strategies,
as well as the anticipated applications of nanoparticles in gene delivery in germ
cells and embryos. Only English language publications were included. Here, we
demonstrate the toxicological effects of ENPs in mammalian germ cells and
developing embryos by considering both in vitro and in vivo experimental models
based on the existing literature. The biodistribution and cellular uptake of ENPs
and the observed toxicities are mostly dependent on ENP size and surface-coating
agents (surface functional groups/surface charge). ENPs have been shown to induce
toxicity via oxidative stress, inflammation and DNA damage in both human and mouse
germ cells. Use of antioxidant, anti-inflammatory drugs and selective metal
chelators would be beneficial against nanoparticle-induced toxicity. Our review
provides the reproductive scientists a mechanistic insight into the
reprotoxicological aspects of ENPs to reliably estimate its potential impact on
human health and help to select/design protective agents to combat ENP-mediated
toxicity. Furthermore, research regarding the detailed mechanism(s) of ENP toxicity
in mammalian germ cells and developing embryos as well as the search for protective
agents to combat ENP-mediated reproductive toxicity is warranted. Furthermore, we
anticipate that investigations into the possibility of applying nanovectors to gene
delivery in germ cells and early embryos will open new horizons in reproductive
biology.
AN - rayyan-553780362
AU - Das, J.
AU - Choi, Y. J.
AU - Song, H.
AU - Kim, J. H.
DO - 10.1093/humupd/dmw020
IS - 5
PY - 2016
SN - 1355-4786 1460-2369
SP - 588-619
ST - Potential toxicity of engineered nanoparticles in mammalian germ cells and
developing embryos: treatment strategies and anticipated applications of
nanoparticles in gene delivery
T2 - HUMAN REPRODUCTION UPDATE
TI - Potential toxicity of engineered nanoparticles in mammalian germ cells and
developing embryos: treatment strategies and anticipated applications of
nanoparticles in gene delivery
VL - 22
Y2 - 9
ID - 8393
ER -
TY - JOUR
AB - This research aimed at reporting the synthesis, characterization and
evaluation of the anti-inflammatory effects of some new biomaterials based on
silver nanoparticles and polyphenols rich natural extracts. A fast and eco-friendly
extracellular biosynthesis of silver nanoparticles (AgNPs), using European black
elderberry (Sambucus nigra - SN, Adoxaceae family) fruit extracts was developed.
The phytosynthesized nanoparticles exhibited an absorbance peak at 426nm,
characteristic for AgNPs and their sizes were ranged from 20 to 80 nm. The anti-
inflammatory properties of AgNPs were assessed in vitro on HaCaT cells exposed to
UVB radiation, in vivo on acute inflammation model and in humans on psoriasis
lesions. In vitro, our results demonstrated the anti-inflammatory effects of
functionalized AgNPs by the decrease of cytokines production induced by UVB
irradiation. In vivo, the pre-administration of AgNPs reduced the edema and
cytokines levels in the paw tissues, early after the induction of inflammation. The
present study also demonstrated the possible use of synthesized AgNPs for the
treatment of psoriasis lesions. (C) 2014 Elsevier B.V. All rights reserved.
AN - rayyan-553780364
AU - David, L.
AU - Moldovan, B.
AU - Vulcu, A.
AU - Olenic, L.
AU - Perde-Schrepler, M.
AU - Fischer-Fodor, E.
AU - Florea, A.
AU - Crisan, M.
AU - Chiorean, I.
AU - Clichici, S.
AU - Filip, G. A.
DO - 10.1016/j.colsurfb.2014.08.018
KW - African Continental Ancestry Group
Fruit
PY - 2014
SN - 0927-7765 1873-4367
SP - 767-777
ST - Green synthesis, characterization and anti-inflammatory activity of silver
nanoparticles using European black elderberry fruits extract
TI - Green synthesis, characterization and anti-inflammatory activity of silver
nanoparticles using European black elderberry fruits extract
VL - 122
Y2 - 10 y3 - 1
ID - 8395
ER -
TY - JOUR
AB - Cisplatin (CDDP) is a well-known platinum-based drug used in the treatment of
various malignancies. However, the widespread side effects that this drug leaves on
normal tissues make its use limited. Since cisplatin is mainly eliminated from the
kidneys, CDDP-induced nephrotoxicity is the most significant dose-limiting
complication attributed to cisplatin, which often leads to dose withdrawal.
Considering the high efficiency of cisplatin in chemotherapy, finding
renoprotective drug delivery systems for this drug is a necessity. In this regard,
we can take advantages of different nanoparticle-based approaches to deliver
cisplatin into tumors either using passive targeting or using specific receptors.
In an effort to find more effective cisplatin-based nano-drugs with less
nephrotoxic effect, the current 2011-2022 review study was conducted to investigate
some of the nanotechnology-based methods that have successfully been able to
mitigate CDDP-induced nephrotoxicity. Accordingly, although cisplatin can cause
renal failures through inducing mitochondria dysfunction, oxidative stress, lipid
peroxidation and endoplasmic reticulum stress, some CDDP-based nano-carriers have
been able to reverse a wide range of these advert effects. Based on the obtained
results, it was found that the use of different metallic and polymeric
nanoparticles can help renal cells to strengthen their antioxidant systems and stay
alive through reducing CDDP-induced ROS generation, inhibiting apoptosis-related
pathways and maintaining the integrity of the mitochondrial membrane. For example,
nanocurcumin could inhibit oxidative stress and acting as a ROS scavenger. CONPs
could reduce lipid peroxidation and pro-inflammatory cytokines. CDDP-loaded silver
nanoparticles (AgNPs) could inhibit mitochondria-mediated apoptosis. In addition,
tea polyphenol-functionalized SeNPs (Se@TE) NPs could mitigate the increased level
of dephosphorylated AKT, phosphorylated p38 MAPK and phosphorylated c-Jun N-
terminal kinase (JNK) induced by cisplatin. Moreover, exosomes mitigated cisplatin-
induced renal damage through inhibiting Bcl2 and increasing Bim, Bid, Bax, cleaved
caspase-9, and cleaved caspase-3. Hence, nanoparticle-based techniques are
promising drug delivery systems for cisplatin so that some of them, such as
lipoplatins and nanocurcumins, have even reached phases 1-3 trials.
AN - rayyan-553780365
AU - Davoudi, M.
AU - Jadidi, Y.
AU - Moayedi, K.
AU - Farrokhi, V.
AU - Afrisham, R.
DO - 10.1186/s12951-022-01718-w
IS - 1
KW - Polymerization
Polymers
Cisplatin
PY - 2022
SN - 1477-3155
ST - Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-
induced nephrotoxicity: a 2011-2022 review
TI - Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-
induced nephrotoxicity: a 2011-2022 review
VL - 20
Y2 - 12 y3 - 1
ID - 8396
ER -
TY - JOUR
AB - SUMMARY: Rheumatoid arthritis (RA) is considered an autoimmune disease
distinguished by chronic synovial membrane inflammation, degraded cartilage, as
well as bone destruction, which lead to joints pain and stiffness. The pathogenesis
of RA involved at least two mechanisms: Cellular proliferation and activation of
glycogen synthase kinase-3β (GSK3β) enzyme. Thus, we tested the hypothesis that the
GSK3binhibitor, TDZD-8, can treat the synovial tissue toward collagen type II
(COII)-mediated RA linked to apoptosis induction and biomarker suppression of
inflammation. Wistar rats were immunized with COII (the model group) for 21 days.
Matched immunized rats were daily injected with TDZD-8 (1 mg/kg; i.p) for three
additional weeks (COII+TDZD- 8).After 42 days of post-immunization, blood and
tissues were collected. Histology (H&E) and immunohistochemistry (CD45; leukocyte
common antigen) images showed that COII induced RA was demonstrated by profound
damage to the synovial tissue and infiltration of the inflammatory cells, which
were substantially ameliorated with TDZD-8. In addition, COII immunization caused
the induction of rheumatoid factor (RF), tumor necrosis factor-alpha (TNF-α),
interleukin-6 (IL-6), and interleukin 1 beta (IL-1β) that were substantially
(p<0.05) suppressed by TDZD-8. Whereas, TDZD-8 augmented the apoptotic biomarker,
Bcl-2-associated X protein (Bax), which was significantly (p<0.05) ameliorated by
RA. We also showed a substantial relationship (p<0.001) between the blood levels of
RF and the synovial tissue levels of TNF-α (r = 0.759), IL-1b (r = 0.969), IL-6 (r
= 0.749), and Bax (r = - 0.914). These results indicate effective treatment of the
injured synovial tissue by TDZD-8 against COII-induced RA in rats, which also
decreases inflammatory biomarkers and augmentation of apoptosis. RESUMEN: La
artritis reumatoide (AR) es una enfermedad autoinmune que se distingue por la
inflamación crónica de la membrana sinovial, el cartílago degradado y la
destrucción de los huesos, lo que provoca dolor y rigidez en las articulaciones. La
patogenia de la AR involucra al menos dos mecanismos: la proliferación celular y la
activación de la enzima glucógeno sintasa quinasa-3b (GSK3β) Por lo tanto, probamos
la hipótesis de que el inhibidor de GSK3β, TDZD-8, puede tratar el tejido sinovial
hacia el colágeno tipo II (COII) - AR mediada por inducción de apoptosis y
supresión de biomarcadores de inflamación. Se inmunizaron ratas Wistar con COII (el
grupo modelo) durante 21 días. Se inyectaron diariamente ratas emparejadas
inmunizadas con TDZD-8 (1 mg / kg; i.p) durante tres semanas adicionales (COII +
TDZD-8). Después de 42 días de post-inmunización, se recolectó sangre y tejidos.
Las imágenes de histología (H&E) e inmunohistoquímica (CD45; antígeno común de
leucocitos) mostraron que la AR inducida por COII presentaba un daño profundo en el
tejido sinovial e infiltración de las células inflamatorias, las que mejoraron con
TDZD-8. Además, la inmunización con COII provocó la inducción de factor reumatoide
(FR), factor de necrosis tumoral alfa (TNF-α), interleucina-6 (IL-6) e interleucina
1 beta (IL-1β) que fueron suprimidos por TDZD-8 de manera significativa (p < 0.05).
Considerando que TDZD-8 aumentó el biomarcador apoptótico, la proteína X asociada a
Bcl-2 (Bax), que fue mejorado (p <0,05) por RA. También se observó una relación
sustancial (p <0,001) entre los niveles sanguíneos de RF y los niveles de tejido
sinovial de TNF-α (r = 0,759), IL-1β (r = 0,969), IL-6 (r = 0,749), y Bax (r = -
0,914). Estos resultados indicaron un tratamiento eficaz del tejido sinovial
lesionado por TDZD-8 contra la AR inducida por COII en ratas, que también disminuye
los biomarcadores inflamatorios y el aumento de la apoptosis.
AN - rayyan-553780367
AU - Dawood, Amal F.
DO - 10.4067/S0717-95022021000100311
IS - 1
KW - Artritis reumatoide
Bax
Inflamación
Inflammation
Modelo de rata
Rat model
Rheumatoid arthritis
TDZD-8
Rats
Collagen Type I
Collagen Type V
Arthritis, Experimental
Apoptosis
Arthritis, Rheumatoid
LA - en
PY - 2021
SN - 0717-9367
SP - 311-317
ST - TDZD-8 suppresses rheumatoid arthritis induced by collagen type II in rats:
potential role of apoptosis and inflammation
TI - TDZD-8 suppresses rheumatoid arthritis induced by collagen type II in rats:
potential role of apoptosis and inflammation
VL - 39
Y2 - 2 y3 - 1
ID - 8397
ER -
TY - JOUR
AB - Background/Aims: A new type of nanoparticle, called NP CB-EDA (Black Carbon
modified with ethylenediamine), is commonly used in the oil industry. In the
literature, few studies are found in biological models, making NP-EDA potential
cytotoxicity in organisms unclear. As its large surface area is capable of
interacting with the biological system, that interaction could lead to factors
harmful to health. The objective of this study was to investigate the cytotoxic
effect of NP CB-EDA on fibroblasts LA-9 at 24 and 48 hours, at different
concentrations of the nanoparticle (1, 50, 250, 500 and 1000 μg/ml). Methods: NP
CB-EDA was characterized by TEM microscopy and its effect on cell viability (MTT
method), cell morphology (optical microscopy), cell membrane (lactate dehydrogenase
release - LDH), oxidative stress pathways (species levels reactive oxygen, ROS and
nitrogen, NOS) and apoptosis/necrosis (flow cytometry) were evaluated. Results: The
results show that NP CB-EDA at concentrations of 500 and 1000 μg/ ml form clusters.
The nanoparticle can be absorbed by cells decreasing cell viability. There was
damage to the cell membrane of fibroblasts LA 9, an increase in the production of
ROS, NOS and pro-inflammatory interleukins TNF-a and IL-6; it was also observed an
increase in % of cells in the state of apoptosis in the two periods analyzed, being
this response more significant in 24 hours, and concentrations of 250, 500 and 1000
μg/ml presenting higher cytotoxicity. Conclusion: The data suggest that NP CB-EDA
in fibroblasts LA9 presents cytotoxic potential, which is associated with oxidative
stress and apoptosis. © 2021 The Author(s).
AN - rayyan-553780368
AU - De Almeida Rodolpho, J. M.
AU - De Godoy, K. F.
AU - Brassolatti, P.
AU - De Lima Fragelli, B. D.
AU - De Castro, C. Aq
AU - Assis, M.
AU - Speglich, C.
AU - Cancino-Bernardi, J.
AU - Longo, E.
AU - De Freitas Anibal, F.
DO - 10.33594/000000382
IS - 3
KW - Apoptosis
Carbon black
Cell viability
Nanoparticle
Oxidative stress
ROS/RNS
Animals
Cell Line
Cytotoxins
Fibroblasts
Mice
Nanoparticles
Oxidative Stress
Soot
carbon nanoparticle
caspase 3
CD4 antigen
interleukin 6
silver nanoparticle
stress activated protein kinase
cytotoxin
nanoparticle
apoptosis
Article
atherogenesis
cell viability
clonogenic assay
colony formation
controlled study
cytotoxicity
EC50
fibroblast
flow cytometry
human
human cell
lung embolism
microscopy
MTT assay
nonhuman
oxidative stress
particle size
zeta potential
animal
cell line
drug effect
metabolism
mouse
pharmacology
soot
African Continental Ancestry Group
PY - 2021
SP - 364-377
ST - Apoptosis and oxidative stress triggered by carbon black nanoparticle in the
LA-9 fibroblast
T2 - Cellular Physiology and Biochemistry
TI - Apoptosis and oxidative stress triggered by carbon black nanoparticle in the
LA-9 fibroblast
85109635875&doi=10.33594%2f000000382&partnerID=40&md5=ee7245fc78db6792a398ab029fb6a
e66
VL - 55
ID - 8398
ER -
TY - JOUR
AB - Engineered nanoparticles offer great promise in many industrial and
biomedical applications, however little information is available about
gastrointestinal toxicity The purpose of this study was to assess the cytotoxicity,
oxidative stress, apoptosis and proinflammatory mediator release induced by ZnO
nanoparticles on human colon carcinoma LoVo cells The biological activity of these
particles was related to their physico-chemical characteristics The physico-
chemical characteristics were evaluated by analytical electron microscopy The
cytotoxicity was determined by growth curves and water-soluble tetrazolium assay
The reactive oxygen species production, cellular glutathione content, changes of
mitochondrial membrane potential and apoptosis cell death were quantified by flow
cytometry. The inflammatory cytokines were evaluated by enzyme-linked
immunoadsorbent assay Treatment with ZnO (5 mu g/cm(2) corresponding to 11 5 mu
g/ml) for 24 h induced on LoVo cells a significant decrease of cell viability,
H2O2/OH center dot increase, 02(-center dot) and GSH decrease, depolarization of
inner mitochondrial membranes, apoptosis and IL-8 release Higher doses induced
about 98% of cytotoxicity already after 24 h of treatment The experimental data
show that oxidative stress may be a key route in inducing the cytotoxicity of ZnO
nanoparticles in colon carcinoma cells Moreover, the study of the relationship
between toxicological effects and physico-chemical characteristics of particles
suggests that surface area does not play a primary role in the cytotoxicity (C)
2010 Elsevier Inc All rights reserved
AN - rayyan-553780369
AU - De Berardis, B.
AU - Civitelli, G.
AU - Condello, M.
AU - Lista, P.
AU - Pozzi, R.
AU - Arancia, G.
AU - Meschini, S.
DO - 10.1016/j.taap.2010.04.012
IS - 3
KW - Humanities
Humanism
Humans
Oxidative Stress
PY - 2010
SN - 0041-008X 1096-0333
SP - 116-127
ST - Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in
human colon carcinoma cells
T2 - TOXICOLOGY AND APPLIED PHARMACOLOGY
TI - Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in
human colon carcinoma cells
VL - 246
Y2 - 8 y3 - 1
ID - 8399
ER -
TY - JOUR
AB - In recent years interest in silver nanoparticles and their applications has
increased mainly because of the important antimicrobial activities of these
nanomaterials, allowing their use in several industrial sectors. However, together
with these applications, there is increasing concerning related to the biological
impacts of the use of silver nanoparticles on a large scale, and the possible risks
to the environment and health. In this scenario, some recent studies have been
published based on the investigation of potential inflammatory effects and diverse
cellular impacts of silver nanoparticles. Another important issue related to
nanoparticle toxicity in biological media is the capacity for increased damage to
the genetic material, since nanoparticles are able to cross cell membranes and
reach the cellular nucleus. In this regard, there is increasing interest in the
analysis of potential nanoparticle genotoxicity, including the effects of different
nanoparticle sizes and methods of synthesis. However, little is known about the
genotoxicity of different silver nanoparticles and their effects on the DNA of
organisms; thus further studies in this field are required. This mini-review aims
to present and to discuss recent publications related to genotoxicity and the
cytotoxicity of silver nanoparticles in order to better understand the possible
applications of these nanomaterials in a safe manner. This present work concludes
that biogenic silver nanoparticles are generally less cyto/genotoxic in vivo
compared with chemically synthesized nanoparticles. Furthermore, human cells were
found to have a greater resistance to the toxic effects of silver nanoparticles in
comparison with other organisms. Copyright (c) 2012 John Wiley & Sons, Ltd.
AN - rayyan-553780372
AU - de Lima, R.
AU - Seabra, A. B.
AU - Duran, N.
DO - 10.1002/jat.2780
IS - 11
KW - Nanoparticles
PY - 2012
SN - 0260-437X 1099-1263
SP - 867-879
ST - Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of
chemically and biogenically synthesized nanoparticles
TI - Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of
VL - 32
Y2 - 11
ID - 8402
ER -
TY - JOUR
AB - In recent years interest in silver nanoparticles and their applications has
increased mainly because of the important antimicrobial activities of these
nanomaterials, allowing their use in several industrial sectors. However, together
with these applications, there is increasing concerning related to the biological
impacts of the use of silver nanoparticles on a large scale, and the possible risks
to the environment and health. In this scenario, some recent studies have been
published based on the investigation of potential inflammatory effects and diverse
cellular impacts of silver nanoparticles. Another important issue related to
nanoparticle toxicity in biological media is the capacity for increased damage to
the genetic material, since nanoparticles are able to cross cell membranes and
reach the cellular nucleus. In this regard, there is increasing interest in the
analysis of potential nanoparticle genotoxicity, including the effects of different
nanoparticle sizes and methods of synthesis. However, little is known about the
genotoxicity of different silver nanoparticles and their effects on the DNA of
organisms; thus further studies in this field are required. This mini-review aims
to present and to discuss recent publications related to genotoxicity and the
cytotoxicity of silver nanoparticles in order to better understand the possible
applications of these nanomaterials in a safe manner. This present work concludes
that biogenic silver nanoparticles are generally less cyto/genotoxic in vivo
compared with chemically synthesized nanoparticles. Furthermore, human cells were
found to have a greater resistance to the toxic effects of silver nanoparticles in
comparison with other organisms.
AN - rayyan-553781773
AU - de Lima, R.
AU - Seabra, A. B.
AU - Durán, N.
DO - 10.1002/jat.2780
IS - 11
J2 - J Appl Toxicol
KW - Animals
Humans
Invertebrates/drug effects
Mice
Mutagens/chemistry/*toxicity
Nanotechnology
Rats
Silver/chemistry/*toxicity
Vertebrates
Nanoparticles
LA - eng
N1 - Department of Biotechnology, University of Sorocaba, Rodovia Raposo Tavares
S/N-km 92,5, CEP 18023-000, Sorocaba, S.P., Brazil. renata.lima@prof.uniso.br
PY - 2012
SP - 867-79
ST - Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of
T2 - Journal of applied toxicology : JAT
TI - Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of
VL - 32
Y2 - 11
ID - 9705
ER -
TY - JOUR
AB - Dietary n-3 polyunsaturated fatty acids (PUFAs) are critical components of
inflammatory response and memory impairment. However, the mechanisms underlying the
sensitizing effects of low n-3 PUFAs in the brain for the development of memory
impairment following inflammation are still poorly understood. In this study, we
examined how a 2-month n-3 PUFAs deficiency from pre-puberty to adulthood could
increase vulnerability to the effect of inflammatory event on spatial memory in
mice. Mice were given diets balanced or deficient in n-3 PUFAs for a 2-month period
starting at post-natal day 21, followed by a peripheral administration of
lipopolysaccharide (LPS), a bacterial endotoxin, at adulthood. We first showed that
spatial memory performance was altered after LPS challenge only in n-3 PUFA-
deficient mice that displayed lower n-3/n-6 PUFA ratio in the hippocampus.
Importantly, long-term depression (LTD), but not long-term potentiation (LTP) was
impaired in the hippocampus of LPS-treated n-3 PUFA-deficient mice. Proinflammatory
cytokine levels were increased in the plasma of both n-3 PUFA-deficient and n-3
PUFA-balanced mice. However, only n-3 PUFA-balanced mice showed an increase in
cytokine expression in the hippocampus in response to LPS. In addition, n-3 PUFA-
deficient mice displayed higher glucocorticoid levels in response to LPS as
compared with n-3 PUFA-balanced mice. These results indicate a role for n-3 PUFA
imbalance in the sensitization of the hippocampal synaptic plasticity to
inflammatory stimuli, which is likely to contribute to spatial memory impairment.
AN - rayyan-553782334
AU - Delpech, J. C.
AU - Thomazeau, A.
AU - Madore, C.
AU - Bosch-Bouju, C.
AU - Larrieu, T.
AU - Lacabanne, C.
AU - Remus-Borel, J.
AU - Aubert, A.
AU - Joffre, C.
AU - Nadjar, A.
AU - Layé, S.
DO - 10.1038/npp.2015.127
IS - 12
J2 - Neuropsychopharmacology
KW - Animals
Animals, Newborn
Corticosterone/blood
Cytokines/blood/genetics
Dendritic Spines/drug effects/pathology/ultrastructure
Fatty Acids, Omega-3/administration & dosage/*metabolism
Hippocampus/metabolism/pathology/physiopathology
Inflammation/blood/chemically induced/*complications
Lipopolysaccharides/toxicity
Long-Term Synaptic Depression/drug effects
Male
Maze Learning/drug effects/physiology
Memory Disorders/*etiology/pathology
Mice
Mice, Inbred C57BL
Neurons/pathology/physiology/ultrastructure
Patch-Clamp Techniques
Silver Staining
Inflammation
Memory
LA - eng
N1 - INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, Bordeaux, France.;
University of Bordeaux, Nutrition et Neurobiologie Intégrée, UMR 1286, Bordeaux,
France.; INRA, Nutrition et Neurobiologie Intégrée, UMR 1286, Bordeaux, France.;
France.
PY - 2015
SP - 2774-87
ST - Dietary n-3 PUFAs Deficiency Increases Vulnerability to Inflammation-Induced
Spatial Memory Impairment
T2 - Neuropsychopharmacology : official publication of the American College of
Neuropsychopharmacology
TI - Dietary n-3 PUFAs Deficiency Increases Vulnerability to Inflammation-Induced
Spatial Memory Impairment
VL - 40
Y2 - 11
ID - 10242
ER -
TY - JOUR
AB - In this study, an adipic acid dihydrazide (ADH)/ tannic acid (TA)-grafted
hyaluronic acid (HA)-based multifunctional hydrogel was synthesized through a
spontaneous amino-yne click reaction and used to promote the improved healing of
infected diabetic wounds. This hydrogel exhibited a range of beneficial properties
such as tunable gelation time, adjustable mechanical properties, pH-sensitive
response characteristics, excellent injectability, the ability to readily adhere to
tissue, and ultra-intimate contact capabilities. Following the encapsulation of
ultrasmall Ag nanoclusters (AgNCs) and deferoxamine loaded polydopamine/ hollow
mesoporous manganese dioxide (PHMD, PDA/H-mMnO2@DFO) nanoparticles, the prepared
hydrogel presented with robust antibacterial, anti-inflammatory, and pro-angiogenic
properties and a desirable smart drug release profile. In this fabricated platform,
PHMD was able to effectively alleviate localized oxidative stress and prolonged
oxygen deprivation via the decomposition of endogenous H2O2 to produce O2. Further
in vivo assays revealed that this hydrogel was capable of facilitating the healing
of infected wounds through the sequential engagement of antibacterial, anti-
inflammatory, and pro-angiogenic activities. Together, this synthesized clickable
environmentally-responsive hydrogel offers great promise as a tool that can be
applied to aid in the healing of chronically infected diabetic wounds and other
inflammatory conditions. © 2022 Elsevier B.V.
AN - rayyan-553780376
AU - Deng, M.
AU - Wu, Y.
AU - Ren, Y.
AU - Song, H.
AU - Zheng, L.
AU - Lin, G.
AU - Wen, X.
AU - Tao, Y.
AU - Kong, Q.
AU - Wang, Y.
DO - 10.1016/j.jconrel.2022.08.053
KW - Amino-yne click reaction
Diabetic wound healing
Environmentally-responsive hydrogel
Smart drug delivery
Deferoxamine
Diabetes Mellitus
Humans
Hyaluronic Acid
Hydrogels
Hydrogen Peroxide
Oxygen
Tannins
Amines
Gelation
Hyaluronic acid
Manganese oxide
adipic acid
deferoxamine
hyaluronic acid
hydrogel
hydrogen peroxide
manganese dioxide
nanoparticle
oxygen
silver
tannin
antiinfective agent
tannin derivative
Click reaction
Diabetic wounds
Property
Responsive hydrogels
Smart drug deliveries
Synthesised
Wound healing
angiogenesis
animal cell
animal experiment
animal model
Article
biocompatibility
controlled study
decomposition
diabetic wound
drug release
encapsulation
gelation
human
in vivo study
nonhuman
oxidative stress
oxygenation
rat
synthesis
wound healing
wound infection
diabetes mellitus
PY - 2022
SP - 613-629
ST - Clickable and smart drug delivery vehicles accelerate the healing of infected
diabetic wounds
TI - Clickable and smart drug delivery vehicles accelerate the healing of infected
diabetic wounds
85137153931&doi=10.1016%2fj.jconrel.2022.08.053&partnerID=40&md5=8976323348ab45c49b
1f8931fc18e11f
VL - 350
ID - 8405
ER -
TY - JOUR
AB - Wound healing is a complex process that is critical in restoring the skin's
barrier function. This process can be interrupted by numerous diseases resulting in
chronic wounds that represent a major medical burden. Such wounds fail to follow
the stages of healing and are often complicated by a pro-inflammatory milieu
attributed to increased proteinases, hypoxia, and bacterial accumulation. The
comprehensive treatment of chronic wounds is still regarded as a significant unmet
medical need due to the complex symptoms caused by the metabolic disorder of the
wound microenvironment. As a result, several advanced medical devices, such as
wound dressings, wearable wound monitors, negative pressure wound therapy devices,
and surgical sutures, have been developed to correct the chronic wound environment
and achieve skin tissue regeneration. Most medical devices encompass a wide range
of products containing natural (e.g., chitosan, keratin, casein, collagen,
hyaluronic acid, alginate, and silk fibroin) and synthetic (e.g., polyvinyl
alcohol, polyethylene glycol, poly[lactic-co-glycolic acid], polycaprolactone,
polylactic acid) polymers, as well as bioactive molecules (e.g., chemical drugs,
silver, growth factors, stem cells, and plant compounds). This review addresses
these medical devices with a focus on biomaterials and applications, aiming to
deliver a critical theoretical reference for further research on chronic wound
healing.
AN - rayyan-553782331
AU - Deng, X.
AU - Gould, M.
AU - Ali, M. A.
DO - 10.1002/jbm.b.35086
IS - 11
J2 - J Biomed Mater Res B Appl Biomater
KW - Alginates
Biocompatible Materials/chemistry
Caseins
*Chitosan
Collagen
*Fibroins
Hyaluronic Acid
Keratins
Peptide Hydrolases
Polymers/therapeutic use
Polyvinyl Alcohol
Silver
Wound Healing
LA - eng
N1 - Centre for Bioengineering & Nanomedicine (Dunedin), Department of Oral
Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.;
Centre for Bioengineering & Nanomedicine (Dunedin), Department of Oral
Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.;
Centre for Bioengineering & Nanomedicine (Dunedin), Department of Oral
Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.
PY - 2022
SP - 2542-2573
ST - A review of current advancements for wound healing: Biomaterial applications
and medical devices
T2 - Journal of biomedical materials research. Part B, Applied biomaterials
TI - A review of current advancements for wound healing: Biomaterial applications
and medical devices
VL - 110
Y2 - 11
ID - 10239
ER -
TY - JOUR
AB - Solar burn reactivation, a rare and idiosyncratic drug reaction, has been
reported with the use of a variety of drugs. This reaction is believed to be the
result of exposure to ultraviolet light during the subsiding phase of an acute
inflammatory reaction. It affects areas of the body that have been previously
sunburned. We describe a 16-year-old girl who was receiving treatment for acute
lymphoblastic leukemia and experienced a second-degree solar burn reactivation
reaction to methotrexate. The patient had a mild sunburn on her face and shoulders
the day she went to the oncology clinic for her interim maintenance chemotherapy
with vincristine 1.5 mg/m2/dose and methotrexate 100 mg/m2/dose. Three days later,
she returned to the clinic with a 2-day history of fever (≤ 100.2°F), nausea,
vomiting, and malaise; the sunburn on her face and shoulders also had become
severe, without further sun exposure. Laboratory results revealed elevated blood
urea nitrogen and serum creatinine concentrations, and her methotrexate level was
elevated at 0.9 mM. The patient was diagnosed with acute renal failure,
dehydration, methotrexate toxicity, and second-degree solar burn reactivation
reaction. She was admitted to the children's hospital and treated with sodium
bicarbonate, acetaminophen with codeine, ondansetron, and silvadene cream. On
hospital day 3, the patient's methotrexate level decreased to less than 0.1 mM. The
sunburn continued to heal, and after a 14-day hospital stay, complicated by a
streptococcal infection, grade 3 mucositis, bacteremia, and mild gastritis and
duodenitis, the patient recovered and was discharged. Use of the Naranjo adverse
drug reaction probability scale indicated a probable relationship (score of 6)
between the patient's solar burn reactivation and methotrexate. Although
methotrexate-induced solar burn reactivation is rare, clinicians should be aware of
this potential adverse reaction and consider delaying administration of
methotrexate by 5-7 days if a patient reports ultraviolet-related erythema in the
past 2-4 days or presents with a notable sunburn.
AN - rayyan-553780379
AU - DeVore, K. J.
DO - 10.1592/phco.30.4.419
IS - 4
KW - Chemotherapy
Dermatology
Methotrexate
Photoreaction
Photosensitivity
Solar burn reactivation
Sunburn
Toxicity
Acute Disease
Adolescent
Antineoplastic Agents, Phytogenic
Blood Urea Nitrogen
Creatinine
Female
Humans
Kidney Failure, Acute
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Treatment Outcome
Ultraviolet Rays
Vincristine
bicarbonate
codeine
creatinine
methotrexate
ondansetron
paracetamol
sulfadiazine silver
vincristine
acute kidney failure
acute lymphoblastic leukemia
adolescent
article
bacteremia
cancer patient
case report
convalescence
cream
creatinine blood level
dehydration
disease severity
duodenitis
erythema
face
female
fever
gastritis
healing
hospital discharge
human
length of stay
maintenance therapy
malaise
mucosa inflammation
Naranjo adverse drug reaction probability scale
nausea
rating scale
shoulder
side effect
Streptococcus infection
sun exposure
sunburn
vomiting
Burns
PY - 2010
SP - 123e-126e
ST - Solar burn reactivation induced by methotrexate
T2 - Pharmacotherapy
TI - Solar burn reactivation induced by methotrexate
77950455599&doi=10.1592%2fphco.30.4.419&partnerID=40&md5=4efb1b355d626781ea01b9addb
c04d22
VL - 30
ID - 8408
ER -
TY - JOUR
AB - Green nanotechnology elucidates highly prioritized anticancer activity. We
synthesized Copper oxide nanoparticles (CuONPs) using leaves of Azadirachta indica
(A. indica) plants and studied the molecular mechanism of cancer cell apoptosis.
After their synthesis, with the help of expository tools like Fourier transform
infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Dynamic
light scattering (DLS) and surface zeta potential we confirmed the successful
synthesis of CuONPs. Here, crystalline structure of green synthesized CuONPs of 36
+/- 8 nm size and spherical shape was able to kill MCF-7 and Hela cells, estimated
by MTT assay. Successful internalization of Cu+2 ions inside the cell was estimated
by the atomic absorption study. Cellular uptake of Cu+2 ions inflicted significant
Reactive Oxygen Species (ROS) generation inside the cancer cells, thereby leading
to DNA fragmentation as observed by DAPI staining. In in vivo model, CuONPs reduced
the breast tumor volume in Balb/C mice and increased the mean survival time through
the alteration of pro-inflammatory cytokines level. In case of both in vivo and in
vitro models, CuONPs altered the pro-inflammatory cytokine level and pro-apoptotic
protein expressions. In future, green synthesized CuONPs might be beneficial for
its application as an anticancer drug in in vivo (mice model) and in vitro, though
further study is needed on its toxicity. (C) 2018 King Saud University. Production
and hosting by Elsevier B.V.
AN - rayyan-553780380
AU - Dey, A.
AU - Manna, S.
AU - Chattopadhyay, S.
AU - Mondal, D.
AU - Chattopadhyay, D.
AU - Raj, A.
AU - Das, S.
AU - Bag, B. G.
AU - Roy, S.
DO - 10.1016/j.jscs.2018.06.011
IS - 2
KW - Apoptosis
PY - 2019
SN - 1319-6103 2212-4640
SP - 222-238
ST - Azadirachta indica leaves mediated green synthesized copper oxide
nanoparticles induce apoptosis through activation of TNF-alpha and caspases
signaling pathway against cancer cells
T2 - JOURNAL OF SAUDI CHEMICAL SOCIETY
TI - Azadirachta indica leaves mediated green synthesized copper oxide
nanoparticles induce apoptosis through activation of TNF-alpha and caspases
signaling pathway against cancer cells
VL - 23
Y2 - 2
ID - 8409
ER -
TY - JOUR
AB - With the increasing focus on healthcare research in the current times,
therapeutic and biomaterial interventions for healing of wounds and mitigation of
wound-associated infections have seen expedited progress. Conventional approaches
consist of release-active gels, which demonstrate leaching of antimicrobials, such
as antibiotics, metal ions, etc. However, these systems suffer from the
disadvantages of burst release, reservoir exhaustion, and associated toxicity. In
this report, intrinsically antimicrobial hydrogel (HyDex) is developed by one-pot
UV crosslinking of methacrylated dextran, polyethylene glycol diacrylate, and
cationic lipophilic methacrylate with varied hydrophobic chain, which displays
broad-spectrum antimicrobial activity, hemostatic ability, and rapid wound closure
efficacy. The optimized hydrogel exhibits potent antimicrobial efficacy against
multidrug-resistant Gram-positive and Gram-negative bacteria as well as against
pathogenic fungus Candida albicans. The HyDex hydrogel shows rapid arrest of
bleeding in mice liver puncture model. The hydrogel kills carbapenem-resistant
Acinetobacter baumannii in a murine model of burn wound infection with >99%
reduction in bacterial burden. Furthermore, this hydrogel displays significant
reduction in inflammatory responses, with accelerated wound healing in rat deep
wound model. Collectively, these results imply the excellent promise held by lead
hydrogel to be developed for tackling deep tissue wounds, notorious infections, and
resulting inflammatory responses.
AN - rayyan-553780381
AU - Dey, R.
AU - Mukherjee, R.
AU - Haldar, J.
DO - 10.1002/adhm.202200536
IS - 15
KW - Wound Healing
PY - 2022
SN - 2192-2640 2192-2659
ST - Photo-Crosslinked Antimicrobial Hydrogel Exhibiting Wound Healing Ability and
Curing Infections In Vivo
T2 - ADVANCED HEALTHCARE MATERIALS
TI - Photo-Crosslinked Antimicrobial Hydrogel Exhibiting Wound Healing Ability and
Curing Infections In Vivo
VL - 11
Y2 - 8
ID - 8410
ER -
TY - JOUR
AB - Various studies have shown that the reproductive organs are highly sensitive
to toxic elements found in the environment. Due to technological progress, the use
of nanoparticles has become more common nowadays. Nanoparticles are used for drug
delivery because their dimensions allow them to circulate throughout the body and
enter directly into the cell. Antimicrobial properties are increasingly used in the
manufacture of medical devices, textiles, food packaging, cosmetics, and other
consumer products. Nanoparticles provide several benefits, but aspects related to
their effects on living organisms and the environment are not well known. This
review summarizes current in vivo, and in vitro animal studies focused on the
evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and
female reproductive health. It can be concluded that higher concentrations of metal
nanoparticles in the male reproductive system can cause a decrease in spermatozoa
motility, viability and disruption of membrane integrity. Histopathological changes
of the testicular epithelium, infiltration of inflammatory cells in the epididymis,
and prostatic hyperplasia have been observed. Nanoparticles in the female
reproductive system caused their accumulation in the ovaries and uterus. Metal
nanoparticles most likely induce polycystic ovary syndrome and follicular atresia,
inflammation, apoptosis, and necrosis also occurred. © 2022 by the authors.
AN - rayyan-553780382
AU - Dianová, L.
AU - Tirpák, F.
AU - Halo, M.
AU - Slanina, T.
AU - Massányi, M.
AU - Stawarz, R.
AU - Formicki, G.
AU - Madeddu, R.
AU - Massányi, P.
DO - 10.3390/toxics10080459
IS - 8
KW - Ag
nanoparticles
reproductive system
TiO2
toxicity
ZnO
silver nanoparticle
apoptosis
epididymis
female genital system
health hazard
histopathology
human
in vitro study
in vivo study
inflammation
inflammatory cell
male genital system
membrane damage
necrosis
ovary follicle atresia
ovary polycystic disease
oxidative stress
prostate hypertrophy
reproductive health
Review
sperm viability
spermatozoon motility
PY - 2022
ST - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
Function of Reproductive Organs
T2 - Toxics
TI - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
85137353498&doi=10.3390%2ftoxics10080459&partnerID=40&md5=66b28ec2158f21e2005fe5d7d
b010943
VL - 10
ID - 8411
ER -
TY - JOUR
AB - Various studies have shown that the reproductive organs are highly sensitive
to toxic elements found in the environment. Due to technological progress, the use
of nanoparticles has become more common nowadays. Nanoparticles are used for drug
delivery because their dimensions allow them to circulate throughout the body and
enter directly into the cell. Antimicrobial properties are increasingly used in the
manufacture of medical devices, textiles, food packaging, cosmetics, and other
consumer products. Nanoparticles provide several benefits, but aspects related to
their effects on living organisms and the environment are not well known. This
review summarizes current in vivo, and in vitro animal studies focused on the
evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and
female reproductive health. It can be concluded that higher concentrations of metal
nanoparticles in the male reproductive system can cause a decrease in spermatozoa
motility, viability and disruption of membrane integrity. Histopathological changes
of the testicular epithelium, infiltration of inflammatory cells in the epididymis,
and prostatic hyperplasia have been observed. Nanoparticles in the female
reproductive system caused their accumulation in the ovaries and uterus. Metal
nanoparticles most likely induce polycystic ovary syndrome and follicular atresia,
inflammation, apoptosis, and necrosis also occurred.
AN - rayyan-553780383
AU - Dianova, L.
AU - Tirpak, F.
AU - Halo, M.
AU - Slanina, T.
AU - Massanyi, M.
AU - Stawarz, R.
AU - Formicki, G.
AU - Madeddu, R.
AU - Massanyi, P.
DO - 10.3390/toxics10080459
IS - 8
PY - 2022
SN - 2305-6304
ST - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
T2 - TOXICS
TI - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
VL - 10
Y2 - 8
ID - 8412
ER -
TY - JOUR
AB - The N-heterocyclic carbene (NHC) precursor 2-pyridin-2-yl-2H-imidazo[1,5-
a]pyridin-4-ylium hexafluorophosphate (1 center dot HPF6) was used to synthesize
various Ag and Au complexes, including [Ag(1)(2)][PF6] (2), [Au(1)(2)][PF6] (3) and
[Au(1)Cl-3] (4). The structure of the silver(I) complex 2 was established via NMR
spectroscopy, mass spectrometry and single crystal X-ray crystallography. The
gold(I)-NHC complex 3 was synthesized via transmetallation of the aforementioned
silver complex and characterized using various spectroscopic methods. Treatment of
3 with Au(SMe2)Cl afforded 4, ostensibly via a disproportionation process. Close
inspection of the solid state structure of 2 revealed that the Ag(I) center adopted
a linear geometry; in contrast, a square planar geometry was observed for the solid
structure of 4. The cytotoxicities of the gold complexes 3 and 4 were tested in
vitro against Human colorectal carcinoma (HCT 116), Human hepatocellular carcinoma
(HepG2), Human breast adenocarcinoma (MCF-7) and Murine melanoma (B16F10). The
measured IC50 values showed that the Au(I) complex 3 was more potent than the
Au(III) complex 4 as well as cisplatin.
AN - rayyan-553780384
AU - Dinda, J.
AU - Samanta, T.
AU - Nandy, A.
AU - Das Saha, K.
AU - Seth, S. K.
AU - Chattopadhyay, S. K.
AU - Bielawskie, C. W.
DO - 10.1039/c3nj01463k
IS - 3
PY - 2014
SN - 1144-0546 1369-9261
SP - 1218-1224
ST - N-heterocyclic carbene supported Au(I) and Au(III) complexes: a comparison of
cytotoxicities
T2 - NEW JOURNAL OF CHEMISTRY
TI - N-heterocyclic carbene supported Au(I) and Au(III) complexes: a comparison of
cytotoxicities
VL - 38
ID - 8413
ER -
TY - JOUR
AB - The current study was designed to investigate the cytotoxicity and
immunomodulatory effects of sol-gel combustion based TiO2 particles (glycine and L-
alanine as reducing agents) of large surface area on RAW 264.7 macrophages. RAW
264.7 macrophages exposed to varying concentrations of TiO2 particles (0.001 to
1000 mu g/ml) were assessed after 24 h and showed a reduced cell viability at 100
and 1000 mu g/ml and increased LDH release at 10 mu g/ml. Furthermore, TiO2
particles (0.1, 1 and 10 mu g/ml) were utilized to assess the immune responses and
intracellular ROS levels on RAW 264.7 macrophages. TiO2 particles at 10 mu g/ml
showed increased mRNA expression of inflammatory cytokines (TNF alpha, IL-1 beta
and IL-6), inflammatory mediators (iNOS and COX -2) and transcription factor (NFO)
similar to that of LPS stimulated macrophages. However, the mRNA expression levels
were found near normal levels at lower concentrations (0.1 and 1 mu g/ml). In
addition, TiO2 particles at 10 mu g/ml also increased the production of
inflammatory cytokines (TNF alpha, IL-1 beta and IL-6) and intracellular ROS levels
in RAW 264.7 macrophages similar to that of LPS stimulated macrophages.
Conclusively, TiO2 particles prepared through this method at a concentration <= 0.1
mu g/ml can be used for various biological applications with minimal
immunomodulatory effects.
AN - rayyan-553780385
AU - Dinesh, P.
AU - Yadav, C. S.
AU - Kannadasan, S.
AU - Rasool, M.
DO - 10.1016/j.tiv.2017.06.006
KW - Titanium
Macrophages
Gels
Polymethyl Methacrylate
PY - 2017
SN - 0887-2333
SP - 92-103
ST - Cytotoxicity and immunomodulatory effects of sol-gel combustion based
titanium dioxide (TiO2) particles of large surface area on RAW 264.7 macrophages
TI - Cytotoxicity and immunomodulatory effects of sol-gel combustion based
titanium dioxide (TiO2) particles of large surface area on RAW 264.7 macrophages
VL - 43
Y2 - 9
ID - 8414
ER -
TY - JOUR
AB - The purpose of this study was to develop a promising wound dressing. Though
chitosan cross-linked with genipin has been widely used as biomaterials, with the
addition of partially oxidized Bletilla striata polysaccharide, the newly developed
material in this study (coded as CSGB) showed less gelling time, more uniform
aperture distribution, higher water retention, demanded mechanical strength and
more L929 cell proliferation compared to the chitosan cross-linked only with
genipin. Owning to partial blocking of free amino groups of chitosan, CSGB revealed
almost no antibacterial activities, thus the bilayer composite of chitosan-silver
nanoparticles (CS-AgG) on CSGB was designed to inhibit microbial invasion. The in
vivo studies indicated that both CSGB and bilayer wound dressing significantly
accelerated the healing rate of cutaneous wounds in mice, and the bilayer exhibited
better mature epidermization with less inflammatory cells on Day 7. Therefore, this
novel bilayer composite has great potential in wound dressing applications. (C)
2016 Elsevier Ltd. All rights reserved.
AN - rayyan-553780386
AU - Ding, L.
AU - Shan, X. D.
AU - Zhao, X. L.
AU - Zha, H. L.
AU - Chen, X. Y.
AU - Wang, J. J.
AU - Cai, C.
AU - Wang, X. J.
AU - Li, G. Y.
AU - Hao, J. J.
AU - Yu, G. L.
DO - 10.1016/j.carbpol.2016.11.040
Wound Healing
Bandages
PY - 2017
SN - 0144-8617 1879-1344
SP - 1538-1547
ST - Spongy bilayer dressing composed of chitosan-Ag nanoparticles and chitosan-
Bletilla striata polysaccharide for wound healing applications
T2 - CARBOHYDRATE POLYMERS
TI - Spongy bilayer dressing composed of chitosan-Ag nanoparticles and chitosan-
Bletilla striata polysaccharide for wound healing applications
VL - 157
Y2 - 2 y3 - 10
ID - 8415
ER -
TY - JOUR
AB - PurposePeri-implantitis is one of the most common inflammatory complications
in dental implantology. Similar to periodontitis, in peri-implantitis, destructive
inflammatory changes take place in the tissues surrounding a dental implant.
Bacterial flora at the failing implant sites resemble the pathogens in periodontal
disease and consist of Gram-negative anaerobic bacteria including Aggregatibacter
actinomycetemcomitans (Aa). Here we demonstrate the effectiveness of a silver
lactate (SL)-containing RGD-coupled alginate hydrogel scaffold as a promising stem
cell delivery vehicle with antimicrobial properties. Materials and MethodsGingival
mesenchymal stem cells (GMSCs) or human bone marrow mesenchymal stem cells
(hBMMSCs) were encapsulated in SL-loaded alginate hydrogel microspheres. Stem cell
viability, proliferation, and osteo-differentiation capacity were analyzed.
ResultsOur results showed that SL exhibited antimicrobial properties against Aa in
a dose-dependent manner, with 0.50 mg/ml showing the greatest antimicrobial
properties while still maintaining cell viability. At this concentration, SL-
containing alginate hydrogel was able to inhibit Aa growth on the surface of Ti
discs and significantly reduce the bacterial load in Aa suspensions. Silver ions
were effectively released from the SL-loaded alginate microspheres for up to 2
weeks. Osteogenic differentiation of GMSCs and hBMMSCs encapsulated in the SL-
loaded alginate microspheres were confirmed by the intense mineral matrix
deposition and high expression of osteogenesis-related genes. ConclusionTaken
together, our findings confirm that GMSCs encapsulated in RGD-modified alginate
hydrogel containing SL show promise for bone tissue engineering with antimicrobial
properties against Aa bacteria in vitro.
AN - rayyan-553780387
AU - Diniz, I. M. A.
AU - Chen, C.
AU - Ansari, S.
AU - Zadeh, H. H.
AU - Moshaverinia, M.
AU - Chee, D.
AU - Marques, M. M.
AU - Shi, S. T.
AU - Moshaverinia, A.
DO - 10.1111/jopr.12316
IS - 2
KW - Mesoderm
PY - 2016
SN - 1059-941X 1532-849X
SP - 105-115
ST - Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD-Coupled
Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for
Peri-Implantitis
T2 - JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY
TI - Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD-Coupled
Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for
Peri-Implantitis
VL - 25
Y2 - 2
ID - 8416
ER -
TY - JOUR
AB - Burn wound progression is an inflammation-driven process where an initial
partial-thickness thermal burn wound can evolve over time to a full-thickness
injury. We have developed an oil-in-water nanoemulsion formulation (NB-201)
containing benzalkonium chloride for use in burn wounds that is antimicrobial and
potentially inhibits burn wound progression. We used a porcine burn injury model to
evaluate the effect of topical nanoemulsion treatment on burn wound conversion and
healing. Anesthetized swine received thermal burn wounds using a 25-cm(2) surface
area copper bar heated to 80 degrees C. Three different concentrations of NB-201
(10, 20, or 40% nanoemulsion), silver sulfadiazine cream, or saline were applied to
burned skin immediately after injury and on days 1, 2, 4, 7, 10, 14, and 18
postinjury. Digital images and skin biopsies were taken at each dressing change.
Skin biopsy samples were stained for histological evaluation and graded. Skin
tissue samples were also assayed for mediators of inflammation. Dermal treatment
with NB-201 diminished thermal burn wound conversion to a full-thickness injury as
determined by both histological and visual evaluation. Comparison of epithelial
restoration on day 21 showed that 77.8% of the nanoemulsion-treated wounds had an
epidermal injury score of 0 compared to 16.7% of the silver sulfadiazine-treated
burns (P = .01). Silver sulfadiazine cream- and saline-treated wounds (controls)
converted to full-thickness burns by day 4. Histological evaluation revealed
reduced inflammation and evidence of skin injury in NB-201-treated sites compared
to control wounds. The nanoemulsion-treated wounds often healed with complete
regrowth of epithelium and no loss of hair follicles (NB-201: 4.8 +/- 2.1, saline:
0 +/- 0, silver sulfadiazine: 0 +/- 0 hair follicles per 4-mm biopsy section, P
< .05). Production of inflammatory mediators and sequestration of neutrophils were
also inhibited by NB-201. Topically applied NB-201 prevented the progression of a
partial-thickness burn wound to full-thickness injury and was associated with a
concurrent decrease in dermal inflammation.
AN - rayyan-553780388
AU - Dolgachev, V. A.
AU - Ciotti, S.
AU - Liechty, E.
AU - Levi, B.
AU - Wang, S. C.
AU - Baker, Jr.
AU - Hemmila, M. R.
DO - 10.1093/jbcr/irab118
IS - 6
KW - Burns
PY - 2021
SN - 1559-047X 1559-0488
SP - 1232-1242
ST - Dermal Nanoemulsion Treatment Reduces Burn Wound Conversion and Improves Skin
Healing in a Porcine Model of Thermal Burn Injury
T2 - JOURNAL OF BURN CARE & RESEARCH
TI - Dermal Nanoemulsion Treatment Reduces Burn Wound Conversion and Improves Skin
Healing in a Porcine Model of Thermal Burn Injury
VL - 42
Y2 - 11
ID - 8417
ER -
TY - JOUR
AB - Objective: To prepare the modified hyaluronic acid viscous hydrogel loaded
with sliver particles and to explore the roles and mechanism of the hydrogel in
healing of full-thickness skin defect wounds with bacterial colonization in mice.
Methods: The experimental research method was adopted. Dopamine modified hyaluronic
acid (HA-DA) and phenylboric acid modified hyaluronic acid (HA-PBA) were prepared,
and their characteristic peaks were detected by Fourier-transform infrared
spectroscopy. Different mass of acrylamides was added to HA-DA and HA-PBA to
prepare the viscous hydrogel with mass fraction of acrylamide in 10%, 15%, and 20%.
The gelation of the viscous hydrogel with mass fraction of acrylamide in 20% was
observed in the state of tilt and inversion at 37 ℃, and the storage modulus and
loss modulus of the above 3 kinds of viscous hydrogels were detected by rotational
rheometer. The sliver-loaded viscous hydrogel was prepared by adding nano silver
ions to the viscous hydrogel with mass fraction of acrylamide in 20%. The
concentration of silver ions released by sliver-loaded viscous hydrogel was
measured by inductively coupled plasma mass spectrometer, and the cumulative
release rate of silver ion was calculated (n=5). The mouse fibroblasts L929 were
divided into phosphate buffered saline (PBS) group, viscous hydrogel group, and
sliver-loaded viscous hydrogel group, which were dealt correspondingly, and the
cell survival was detected by cell counting kit 8 method after 1, 2, and 3 d of
culture (n=5). Twenty-four male C57BL/6 mice aged 6-8 weeks were selected, and
forty-eight full-thickness skin defect wounds were inflicted and inoculated with
the mixture of Escherichia coli and Staphylococcus aureus in the back of the mice,
with two wounds in each mouse. The wounds were divided into normal saline group,
viscous hydrogel group, and sliver-loaded viscous hydrogel group, which were dealt
correspondingly, with 16 wounds in each group, and two wounds in each mouse were
divided into different groups. On post injury day (PID) 3, 7, 10, and 14, the wound
healing was observed and the wound healing rate was calculated. On PID 3, the
colony forming units of Escherichia coli and Staphylococcus aureus in wounds were
observed and counted. On PID 14, the epithelized epidermal thickness and the
optical density of collagen fiber in wounds were observed and analyzed after
hematoxylin eosin staining and Masson staining, respectively. On PID 3, 7, and 10,
the expressions of tumor necrosis factor α (TNF-α), transforming growth factor β(1)
(TGF-β(1)), and vascular endothelial growth factor (VEGF) were detected by
immunohistochemistry. The number of wounds in each index detecting at each time
point was four. Data were statistically analyzed with analysis of variance for
factorial design, one-way analysis of variance, and Bonferroni correction. Results:
The characteristic peaks of HA-PBA were detected at the wave numbers of 1 369 and 1
425 cm(-1), indicating that phenylboric acid had been successfully grafted on
hyaluronic acid, and the characteristic peaks of HA-DA were detected at the wave
numbers of 1 516 and 1 431 cm(-1), indicating that dopamine had been successfully
grafted on hyaluronic acid. The viscous hydrogel with mass fraction of acrylamide
in 20% maintained the stable and no-flow condition of gelation in the state of tilt
and inversion at 37 ℃. The storage modulus and loss modulus of the viscous hydrogel
increased with the increase of acrylamide content, the storage modulus and loss
modulus of the 3 kinds of viscous hydrogels had no obvious changes with the
increase of the oscillation frequency or time, and the storage modulus of the 3
kinds of acrylamide hydrogels were greater than the loss modulus. The release of
silver ion in the sliver-loaded viscous hydrogel lasted for 7 days, and the
cumulative release rate of silver ion was up to 65%. After 1, 2, and 3 d of
culture, the cell survival rates in sliver-loaded viscous hydrogel group were
significantly lower than those in PBS group and viscous hydrogel group (P<0 05 or
P<0.01), while after 1 d of culture, the cell survival rate in viscous hydrogel
group was significantly lower than that in PBS group (P<0.01). With extension of
time after injury, the wounds of mice in the 3 groups shrank gradually. On PID 3,
7, 10, and 14, the wound healing rates in sliver-loaded viscous hydrogel group were
(53.0±3.6)%, (75.3±6.9)%, (93.3±1.2)%, and (96.7±0.8)%, which were significantly
higher than (21.8±6.4)%, (53.9±8.2)%, (72.0±7.8)%, and (92.5±0.4)% in normal saline
group (P<0.01). On PID 3 and 14, the wound healing rates in sliver-loaded viscous
hydrogel group were significantly higher than (43.5±2.4)% and (94.1±1.5)% in
viscous hydrogel group (P<0.05). On PID 3 and 10, the wound healing rates in
viscous hydrogel group were significantly higher than those in normal saline group
(P<0.01). On PID 3, the colony forming units of two bacteria in wound of sliver-
loaded viscous hydrogel group were significantly less than those in normal saline
group and viscous hydrogel group (P<0.01), while the colony forming units of two
bacteria in wound of viscous hydrogel group were significantly less than those in
normal saline group (P<0.05). On PID 14, the wounds were basically epithelialized
and the epidermis was thicker, with collagen protein content being increased
significantly and more orderly arranged collagen in sliver-loaded viscous hydrogel
group compared with those in the other 2 groups. On PID 14, the epidermal thickness
in wounds of sliver-loaded viscous hydrogel group was significantly increased
compared with that in the other two groups (P<0.05), and the optical density of
collagen fiber was significantly increased compared with those in normal saline
group (P<0.05). On PID 3, the expressions of TGF-β(1) and VEGF in wounds of sliver-
loaded viscous hydrogel group were significantly higher than those in normal saline
group (P<0.05 or P<0.01), while the expression of VEGF in wounds of viscous
hydrogel group was significantly higher than that in normal saline group (P<0.01).
On PID 7, the expression of TGF-β(1) in wounds of sliver-loaded viscous hydrogel
group was significantly higher than that in the other 2 groups (P<0.01), and the
expression of VEGF was significantly higher than that in normal saline group
(P<0.01). On PID 10, the expression of TNF-α in wounds of sliver-loaded viscous
hydrogel group was significantly lower than that in normal saline group (P<0.05),
the expressions of TGF-β(1) and VEGF in wounds of sliver-loaded viscous hydrogel
group were significantly higher than those in normal saline group (P<0.05 or
P<0.01), and the expression of VEGF in wounds of sliver-loaded viscous hydrogel
group was significantly higher than that in viscous hydrogel group (P<0.05).
Conclusions: The sliver-loaded viscous hydrogel prepared in this study has good
stability and elasticity, which can continuously release silver ions and help to
accelerate the healing of full-thickness defect wounds with bacterial colonization
in mice. Besides, the sliver-loaded viscous hydrogel has low biological toxicity
and can promote re-epithelialization, collagen deposition as well as angiogenesis
of wounds, which may be related to the infiltration and regression of inflammatory
cells.
AN - rayyan-553782419
AU - Dong, Y. Q.
AU - Li, L. L.
AU - Zhu, X. R.
AU - Feng, L. B.
AU - Jia, K. Y.
AU - Guo, R.
AU - Cheng, B.
DO - 10.3760/cma.j.cn501120-20210906-00304
IS - 11
J2 - Zhonghua Shao Shang Za Zhi
KW - Animals
Bacteria
*Hydrogels
Male
Mice
Mice, Inbred C57BL
*Vascular Endothelial Growth Factor A
Wound Healing
LA - chi
N1 - The First School of Clinical Medicine, Southern Medical University, Guangzhou
510515, China.; The First School of Clinical Medicine, Southern Medical University,
Guangzhou 510515, China.; Department of Burns and Plastic Surgery, General Hospital
of Southern Theater Command of PLA, Guangzhou 510010, China.; Department of
Biomedical Engineering of Jinan University, Guangzhou 510632, China.; The First
School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China.;
Department of Biomedical Engineering of Jinan University, Guangzhou 510632, China.;
The First School of Clinical Medicine, Southern Medical University, Guangzhou
510515, China.
PY - 2021
SN - 1009-2587 (Print)
SP - 1036-1047
ST - [Preparation and roles of sliver-loaded viscous hydrogel in healing of full-
thickness skin defect wounds with bacterial colonization in mice]
T2 - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of
burns
TI - [Preparation and roles of sliver-loaded viscous hydrogel in healing of full-
thickness skin defect wounds with bacterial colonization in mice]
VL - 37
Y2 - 11 y3 - 20
ID - 10326
ER -
TY - JOUR
AB - Here we describe development of a silicone rubber/stainless steel mesh cage
implant system, much like that used to assess biocompatibility of biomaterials [1],
for easy removal of injectable polymer micro spheres in vivo. The sterile cage has
a type 316 stainless steel mesh size (38 gm) large enough for cell penetration and
free fluid flow in vivo but small enough for microsphere retention, and a silicone
rubber shell for injection of the microspheres. Two model drugs, the poorly soluble
steroid, triamcinolone acetonide, and the highly water-soluble luteinizing hormone-
releasing hormone (LHRH) peptide superagonist, leuprolide, were encapsulated in
PLGA microspheres large enough (63-90 mu m) to be restrained' by the cage implant
in vivo. The in vitro release from both formulations was followed by ultra
performance liquid chromatography (UPLC) with and without the cage in a standard
release media, PBS pH 7.4 + 0.02% Tween 80 0.05% sodium azide, at 37 degrees C.
Pharmacokinetics (PM) in rats was assessed after SC injection or SC in-cage
implantation of microspheres with plasma analysis by LC-MS/MS or EIA. Tr-A and
leuprolide in vitro release was largely unaffected after the initial, burst
irrespective of the cage or test tube incubation vessel and release was much slower
than observed in vivo for both drugs. Moreover, Tr-A and leuprolide
pharmacokinetics with and without the cage were highly similar during the 2-3 week
release duration before a significant inflammatory response was caused by the cage
implant. Hence, the PM-validated cage implant provides a simple means to recover
and evaluate the microsphere drug carriers in vivo during a time window of at least
a few weeks in order to characterize the polymer microsphere release and erosion
behavior in vivo. This approach may facilitate development of mechanism-based in
vitro/in vivo correlations and enable development of more accurate and useful in
vitro release tests. (C) 2016 Elsevier Ltd. All rights reserved.
AN - rayyan-553780389
AU - Doty, A. C.
AU - Hirota, K.
AU - Olsen, K. F.
AU - Sakamoto, N.
AU - Ackermann, R.
AU - Feng, M. R.
AU - Wang, Y.
AU - Choi, S.
AU - Qu, W.
AU - Schwendeman, A.
AU - Schwendeman, S. P.
DO - 10.1016/j.biomaterials.2016.07.041
KW - Microspheres
PY - 2016
SN - 0142-9612 1878-5905
SP - 88-96
ST - Validation of a cage implant system for assessing in vivo performance of
long-acting, release microspheres
T2 - BIOMATERIALS
TI - Validation of a cage implant system for assessing in vivo performance of
long-acting, release microspheres
VL - 109
Y2 - 12
ID - 8418
ER -
TY - JOUR
AB - The use of selective barriers as resorbable membranes has become a routine
clinical procedure for guided bone regeneration. Therefore, the production of
membranes with a low inflammatory potential during their resorption process has
become the goal of a considerable number of researches. Aim: The purpose of the
present study was to evaluate the biocompatibility of poly (L- lactic acid) (PLLA)
and biocelulose membranes (BC) inserted in the subcutaneous tissue on the dorsum of
rats. Methods: Fifteen animals underwent surgical procedures for the insertion of 4
types of membranes: COL (Collagen membrane) Control Group; BC (Biocellulose
membrane); BCAg (Biocellulose membrane impregnated with Silver); PLLA (Poly (L-
lactic acid) membrane). All membrane types were inserted into each animal. Animals
were euthanized after 3, 7, and 15 days of the surgical procedure. Descriptive
histological analyses were carried out to investigate host tissue reaction to
membrane presence by assessing the anti-inflammatory process composition associated
with the membrane resorption and the presence of foreign-body reaction or
encapsulation. Results: The BC membranes showed a higher degree of inflammation and
poor pattern of integration with the surrounding tissues than the PLLA and COL
membranes. Conclusion: The PLLA and COL membranes present better biocompatibility
than the BC membranes
AN - rayyan-553780390
AU - Doval Neto, José
AU - Marques, Rodrigo Fernando Costa
AU - Motta, Adriana Cristina
AU - Duek, Eliana Aparecida de Rezende
AU - Oliveira, Guilherme José Pimentel Lopes de
AU - Marcantonio, Cláudio
DO - 10.20396/bjos.v21i00.8670616
KW - Biocompatible materials
Celulose
Inflammation
Membranes
Polyesters
RNA, Messenger
Polyglactin 910
Poly I-C
Lactic Acid
Poly A-U
LA - en
PY - 2022
SN - 1677-3217
SP - e220616-e220616
ST - Analysis of the biocompatibility of a biocelulose and a poly L- lactic acid
membrane
T2 - Braz. j. oral sci
TI - Analysis of the biocompatibility of a biocelulose and a poly L- lactic acid
membrane
UR - https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/
8670616/29880
VL - 21
Y2 - 12 y3 - 1
ID - 8419
ER -
TY - JOUR
AB - This work describes measurement methodology on acrylic acid-based hydrogels
with nanogold and starch, including evaluation of pro-inflammatory activity and
cytotoxicity by MIT reduction assay, swelling capacity, tensile strength using
Brookfield texture analyzer, surface morphology by scanning electron microscopy,
structure by FT-IR spectroscopy, crystallinity by X-ray diffraction and hydrogel
behaviour in simulated body fluids. The modified polymers exhibited approx. 75%
lower deformation than unmodified polymer. Hydrogels showed buffering properties in
simulated body fluids. Hydrogels containing 5-10% starch solution did not exhibit
cytotoxicity. Cells exposed to unmodified hydrogel and to hydrogels containing 5%
and 10% starch solutions were characterized by the desired morphology. Importantly,
hydrogel nanocomposites have not showed pro-inflammatory activity. It was proved
that highly developed specific surface area of modified hydrogels affected their
sorption properties. Nanogold present in nanocomposites did not affect directly
their physicochemical properties but, due to its features, antibacterial activity
of so modified materials could be enhanced. (C) 2020 The Author(s). Published by
Elsevier Ltd.
AN - rayyan-553780391
AU - Drabczyk, A.
AU - Kudlacik-Kramarczyk, S.
AU - Tyliszczak, B.
AU - Rudnicka, K.
AU - Urbaniak, M.
AU - Michlewska, S.
AU - Krolczyk, J. B.
AU - Gajda, P.
AU - Pielichowski, K.
DO - 10.1016/j.measurement.2020.107608
KW - Starch Synthase
Starch
Hydrogel
Hydrogels
PY - 2020
SN - 0263-2241 1873-412X
ST - Measurement methodology toward determination of structure-property
relationships in acrylic hydrogels with starch and nanogold designed for biomedical
applications
T2 - MEASUREMENT
TI - Measurement methodology toward determination of structure-property
relationships in acrylic hydrogels with starch and nanogold designed for biomedical
applications
VL - 156
Y2 - 5
ID - 8420
ER -
TY - JOUR
AB - Antimicrobial resistance poses a significant threat to public health and
social development worldwide. This study aimed to investigate the effectiveness of
silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections.
Eco-friendly spherical AgNPs were synthesized using rutin at room temperature. The
biocompatibility of both polyvinyl pyrrolidone (PVP) and mouse serum (MS)-
stabilized AgNPs was evaluated at 20 μg/mL and showed a similar distribution in
mice. However, only MS-AgNPs significantly protected mice from sepsis caused by the
multidrug-resistant Escherichia coli (E. coli) CQ10 strain (p = 0.039). The data
revealed that MS-AgNPs facilitated the elimination of Escherichia coli (E. coli) in
the blood and the spleen, and the mice experienced only a mild inflammatory
response, as interleukin-6, tumor necrosis factor-α, chemokine KC, and C-reactive
protein levels were significantly lower than those in the control group. The
results suggest that the plasma protein corona strengthens the antibacterial effect
of AgNPs in vivo and may be a potential strategy for combating antimicrobial
resistance. Copyright © 2023 Du, Wang, Zhang, Chen, Deng, He, Tang, Deng and Ren.
AN - rayyan-553780393
AU - Du, H.
AU - Wang, X.
AU - Zhang, H.
AU - Chen, H.
AU - Deng, X.
AU - He, Y.
AU - Tang, H.
AU - Deng, F.
AU - Ren, Z.
DO - 10.3389/fmicb.2023.1153147
KW - multidrug-resistant bacteria
pro-inflammatory cytokine
protein corona
sepsis
C reactive protein
creatinine
interleukin 6
povidone
silver nanoparticle
acute toxicity
animal experiment
animal tissue
antisepsis
Article
bacterial load
biocompatibility
cell infiltration
controlled study
Escherichia coli
gene ontology
hemolysis
histology
in vitro study
inflammation
LD50
liver cell
male
mass spectrometry
methicillin resistant Staphylococcus aureus infection
morbidity
mortality
mortality rate
mouse
nonhuman
public health
spleen
Blood Proteins
PY - 2023
ST - Serum protein coating enhances the antisepsis efficacy of silver
nanoparticles against multidrug-resistant Escherichia coli infections in mice
T2 - Frontiers in Microbiology
TI - Serum protein coating enhances the antisepsis efficacy of silver
nanoparticles against multidrug-resistant Escherichia coli infections in mice
85161218779&doi=10.3389%2ffmicb.2023.1153147&partnerID=40&md5=d0f7cd708c38bcacdf122
a14fcd4469a
VL - 14
ID - 8422
ER -
TY - JOUR
AB - The toxicity mechanism of nanoparticles on vertebrate cardiovascular system
is still unclear, especially on the low-level exposure. This study was to explore
the toxic effect and mechanisms of low-dose exposure of silica nanoparticles
(SiNPs) on cardiac function in zebrafish embryos via the intravenous
microinjection. The dosage of SiNPs was based on the no observed adverse effect
level (NOAEL) of malformation assessment in zebrafish embryos. The mainly cardiac
toxicity phenotypes induced by SiNPs were pericardial edema and bradycardia but had
no effect on atrioventricular block. Using o-Dianisidine for erythrocyte staining,
the cardiac output of zebrafish embryos was decreased in a dose-dependent manner.
Microarray analysis and bioinformatics analysis were performed to screen the
differential expression genes and possible pathway involved in cardiac function.
SiNPs induced whole-embryo oxidative stress and neutrophil-mediated cardiac
inflammation in Tg(mpo:GFP) zebrafish. Inflammatory cells were observed in atrium
of SiNPs-treated zebrafish heart by histopathological examination. In addition, the
expression of TNNT2 protein, a cardiac contraction marker in heart tissue had been
down-regulated compared to control group using immunohistochemistry. Confirmed by
qRT-PCR and western blot assays, results showed that SiNPs inhibited the calcium
signaling pathway and cardiac muscle contraction via the down-regulated of related
genes, such as ATPase-related genes (atp2a1l, atp1b2b, atp1a3b), calcium channel-
related genes (cacna1ab, cacna1da) and the regulatory gene tnnc1a for cardiac
troponin C. Moreover, the protein level of TNNT2 was decreased in a dose-dependent
manner. For the first time, our results demonstrated that SiNPs induced cardiac
dysfunction via the neutrophil-mediated cardiac inflammation and cardiac
contraction in zebrafish embryos.
AN - rayyan-553780394
AU - Duan, J. C.
AU - Yu, Y.
AU - Li, Y.
AU - Li, Y. B.
AU - Liu, H. C.
AU - Jing, L.
AU - Yang, M.
AU - Wang, J.
AU - Li, C. Q.
AU - Sun, Z. W.
DO - 10.3109/17435390.2015.1102981
IS - 5
KW - Inflammation
PY - 2016
SN - 1743-5390 1743-5404
SP - 575-585
ST - Low-dose exposure of silica nanoparticles induces cardiac dysfunction via
neutrophil-mediated inflammation and cardiac contraction in zebrafish embryos
T2 - NANOTOXICOLOGY
TI - Low-dose exposure of silica nanoparticles induces cardiac dysfunction via
neutrophil-mediated inflammation and cardiac contraction in zebrafish embryos
VL - 10
Y2 - 5 y3 - 27
ID - 8423
ER -
TY - JOUR
AB - Nanoparticles (NPs) are widely used in consumer and medicinal products. The
high prevalence of nanoparticles in the environment raises concerns regarding their
effects on human health, but there is limited knowledge about how NPs interact with
cells or tissues. Because the European Union has called for a substantial reduction
of animal experiments for scientific purposes (Directive 2010/63), increased
efforts are required to develop in vitro models to evaluate potentially hazardous
agents. Here, we describe a new cell-based biosensor for the evaluation of NPs
cytotoxicity. The new biosensor is based on transgenic human hepatoblastoma cells
(HepG2) that express a secreted form of alkaline phosphatase (SEAP) as a reporter
protein whose expression is induced upon activation of a stress response pathway
controlled by the transcription regulator nuclear factor-kappa B (NF-kappa B). The
NF-kappa B_HepG2 sensor cells were cultured in a Matrigel-based three dimensional
environment to simulate the in vivo situation. The new biosensor cells offer the
advantage of generating fast and reproducible readout at lower concentrations and
shorter incubation time than conventional viability assays, avoid possible
interaction between nanomaterials and assay compounds, therefore, minimize
generation of false positive or negative results and indicate mechanism of toxicity
through NF-kappa B signaling. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
AN - rayyan-553780395
AU - Dubiak-Szepietowska, M.
AU - Karczmarczyk, A.
AU - Winckler, T.
AU - Feller, K. H.
DO - 10.1016/j.tox.2016.09.012
PY - 2016
SN - 0300-483X
SP - 60-69
ST - A cell-based biosensor for nanomaterials cytotoxicity assessment in three
dimensional cell culture
T2 - TOXICOLOGY
TI - A cell-based biosensor for nanomaterials cytotoxicity assessment in three
dimensional cell culture
VL - 370
Y2 - 8 y3 - 31
ID - 8424
ER -
TY - JOUR
AB - Fc-related antibody activities, such as antibody-dependent cellular
cytotoxicity (ADCC), or more broadly, antibody-mediated cellular viral inhibition
(ADCVI), play a role in curbing early SIV viral replication, are enriched in human
long-term infected nonprogressors, and could potentially contribute to protection
from infection. However, little is known about the mechanism by which such humoral
immune responses are naturally induced following infection. Here, we focused on the
early evolution of the functional antibody response, largely driven by the Fc
portion of the antibody, in the context of the evolving binding and neutralizing
antibody response, which is driven mainly by the antibody-binding fragment (Fab).
We show that ADCVI/ ADCC-inducing responses in humans are rapidly generated
following acute HIV-1 infection, peak at approximately 6 months postinfection, but
decay rapidly in the setting of persistent immune activation, as Fab-related
activities persistently increase. Moreover, the loss of Fc activity occurred in
synchrony with a loss of HIV-specific IgG3 responses. Our data strongly suggest
that Fc- and Fab-related antibody functions are modulated in a distinct manner
following acute HIV infection. Vaccination strategies intended to optimally induce
both sets of antiviral antibody activities may, therefore, require a fine tuning of
the inflammatory response.
AN - rayyan-553780397
AU - Dugast, A. S.
AU - Stamatatos, L.
AU - Tonelli, A.
AU - Suscovich, T. J.
AU - Licht, A. F.
AU - Mikell, I.
AU - Ackerman, M. E.
AU - Streeck, H.
AU - Klasse, P. J.
AU - Moore, J. P.
AU - Alter, G.
DO - 10.1002/eji.201344305
IS - 10
KW - Antiviral Agents
PY - 2014
SN - 0014-2980 1521-4141
SP - 2925-2937
ST - Independent evolution of Fc- and Fab-mediated HIV-1-specific antiviral
antibody activity following acute infection
T2 - EUROPEAN JOURNAL OF IMMUNOLOGY
TI - Independent evolution of Fc- and Fab-mediated HIV-1-specific antiviral
antibody activity following acute infection
VL - 44
Y2 - 10
ID - 8426
ER -
TY - JOUR
AB - For the last years scientific community has witnessed a rapid development of
novel types of biomaterials, which properties made them applicable in numerous
fields of medicine. Although nanosilver, well-known for its antimicrobial, anti-
angiogenic, anti-inflammatory and anticancer activities, as well as hyaluronic
acid, a natural polysaccharide playing a vital role in the modulation of tissue
repair, signal transduction, angiogenesis, cell motility and cancer metastasis, are
both thoroughly described in the literature, their complexes are still a novel
topic. In this review we introduce the most recent research about the synthesis,
properties, and potential applications of HA-nanosilver composites. We also make an
attempt to explain the variety of mechanisms involved in their action. Finally, we
present biocompatible and biodegradable complexes with bactericidal activity and
low cytotoxicity, which properties suggest their suitability for the prophylaxis
and therapy of chronic wounds, as well as analgetic therapies, anticancer
strategies and the detection of chemical substances and malignant cells. Cited
studies reveal that the usage of hyaluronic acid-silver nanocomposites appears to
be efficient and safe in clinical practice. © 2021 by the authors. Licensee MDPI,
Basel, Switzerland.
AN - rayyan-553780398
AU - Dulińska-Litewka, J.
AU - Dykas, K.
AU - Felkle, D.
AU - Karnas, K.
AU - Khachatryan, G.
AU - Karewicz, A.
DO - 10.3390/ma15010234
IS - 1
KW - Biopolymers
Hyaluronic acid
Nanocomposites
Polysaccharides
Biocompatibility
Chemical detection
Diseases
Organic acids
Signal transduction
Angiogenesis
Anticancer activities
Nano silver
Natural polysaccharide
Property
Scientific community
Silver nanocomposites
Tissue repair
PY - 2022
ST - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
T2 - Materials
TI - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
85122931311&doi=10.3390%2fma15010234&partnerID=40&md5=c67a9f3a5af61ee87db7e54f744a2
b8b
VL - 15
ID - 8427
ER -
TY - JOUR
AB - For the last years scientific community has witnessed a rapid development of
novel types of biomaterials, which properties made them applicable in numerous
fields of medicine. Although nanosilver, well-known for its antimicrobial, anti-
angiogenic, anti-inflammatory and anticancer activities, as well as hyaluronic
acid, a natural polysaccharide playing a vital role in the modulation of tissue
repair, signal transduction, angiogenesis, cell motility and cancer metastasis, are
both thoroughly described in the literature, their complexes are still a novel
topic. In this review we introduce the most recent research about the synthesis,
properties, and potential applications of HA-nanosilver composites. We also make an
attempt to explain the variety of mechanisms involved in their action. Finally, we
present biocompatible and biodegradable complexes with bactericidal activity and
low cytotoxicity, which properties suggest their suitability for the prophylaxis
and therapy of chronic wounds, as well as analgetic therapies, anticancer
strategies and the detection of chemical substances and malignant cells. Cited
studies reveal that the usage of hyaluronic acid-silver nanocomposites appears to
be efficient and safe in clinical practice.
AN - rayyan-553780399
AU - Dulinska-Litewka, J.
AU - Dykas, K.
AU - Felkle, D.
AU - Karnas, K.
AU - Khachatryan, G.
AU - Karewicz, A.
DO - 10.3390/ma15010234
IS - 1
PY - 2022
SN - 1996-1944
ST - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
T2 - MATERIALS
TI - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
VL - 15
Y2 - 1
ID - 8428
ER -
TY - JOUR
AB - BackgroundSepsis biomarker panels that provide diagnostic and prognostic
discrimination in sepsis patients would be transformative to patient care. We
assessed the mortality prediction and diagnostic discriminatory accuracy of two
biomarkers reflective of cell death (apoptosis), circulating cell-free DNA (cfDNA),
and nucleosomes.MethodsThe cfDNA and nucleosome levels were assayed in plasma
samples acquired in patients admitted from four emergency departments with
suspected sepsis. Subjects with non-infectious systemic inflammatory response
syndrome (SIRS) served as controls. Samples were acquired at enrollment (T0) and
24h later (T24). We assessed diagnostic (differentiating SIRS from sepsis) and
prognostic (28-day mortality) predictive power. Models incorporating procalcitonin
(diagnostic prediction) and APACHE II scores (mortality prediction) were
generated.ResultsTwo hundred three subjects were included (107 provided
procalcitonin measurements). Four subjects exhibited uncomplicated sepsis, 127
severe sepsis, 35 septic shock, and 24 had non-infectious SIRS. There were 190-
survivors and 13 non-survivors. Mortality prediction models using cfDNA,
nucleosomes, or APACHEII yielded AUC values of 0.61, 0.75, and 0.81, respectively.
A model combining nucleosomes with the APACHE II score improved the AUC to 0.84.
Diagnostic models distinguishing sepsis from SIRS using procalcitonin, cfDNA(T0),
or nucleosomes(T0) yielded AUC values of 0.64, 0.65, and 0.63, respectively. The
three parameter model yielded an AUC of 0.74.ConclusionsTo our knowledge, this is
the first head-to-head comparison of cfDNA and nucleosomes in diagnosing sepsis and
predicting sepsis-related mortality. Both cfDNA and nucleosome concentrations
demonstrated a modest ability to distinguish sepsis survivors and non-survivors and
provided additive diagnostic predictive accuracy in differentiating sepsis from
non-infectious SIRS when integrated into a diagnostic prediction model including
PCT and APACHE II. A sepsis biomarker strategy incorporating measures of the
apoptotic pathway may serve as an important component of a sepsis diagnostic and
mortality prediction tool.
AN - rayyan-553780400
AU - Duplessis, C.
AU - Gregory, M.
AU - Frey, K.
AU - Bell, M.
AU - Truong, L.
AU - Schully, K.
AU - Lawler, J.
AU - Langley, R. J.
AU - Kingsmore, S. F.
AU - Woods, C. W.
AU - Rivers, E. P.
AU - Jaehne, A. K.
AU - Quackenbush, E. B.
AU - Fowler, V. G.
AU - Tsalik, E. L.
AU - Clark, D.
DO - 10.1186/s40560-018-0341-5
KW - Discrimination (Psychology)
PY - 2018
SN - 2052-0492
ST - Evaluating the discriminating capacity of cell death (apoptotic) biomarkers
in sepsis
T2 - JOURNAL OF INTENSIVE CARE
TI - Evaluating the discriminating capacity of cell death (apoptotic) biomarkers
in sepsis
VL - 6
Y2 - 11 y3 - 13
ID - 8429
ER -
TY - JOUR
AB - Following spinal cord injury (SCI), the population of mature oligodendrocytes
undergoes substantial cell death; promoting their preservation and replacement is a
viable strategy for preserving axonal integrity and white matter repair in the
injured spinal cord. Dramatic upregulation of matrix chondroitin sulfate
proteoglycans (CSPGs) is shown to pose an obstacle to endogenous repair processes,
and targeting CSPGs improves functional recovery after SCI. However, the cellular
and molecular mechanisms underlying the inhibitory effects of CSPGs remain largely
undefined. Modulation of CSPGs specific signaling receptors, leukocyte common
antigen-related (LAR), and protein tyrosine phosphatase-sigma (PTP sigma) allows us
to uncover the role and mechanisms of CSPGs in regulating oligodendrocytes in SCI.
Here, utilizing specific functionally blocking peptides in a clinically relevant
model of contusive/compressive SCI in the rat, we demonstrate that inhibition of
PTP sigma and LAR receptors promotes oligodendrogenesis by endogenous precursor
cells, attenuates caspase 3-mediated cell death in mature oligodendrocytes, and
preserves myelin. In parallel in vitro systems, we have unraveled that CSPGs
directly induce apoptosis in populations of neural precursor cells and
oligodendrocyte progenitor cells and limit their ability for oligodendrocyte
differentiation, maturation, and myelination. These negative effects of CSPGs are
mediated through the activation of both LAR and PTP sigma receptors and the
downstream Rho/ROCK pathway. Thus, we have identified a novel inhibitory role for
PTP sigma and LAR in regulating oligodendrocyte differentiation and apoptosis in
the injured adult spinal cord and a new feasible therapeutic strategy for
optimizing endogenous cell replacement following SCI.
AN - rayyan-553780402
AU - Dyck, S.
AU - Kataria, H.
AU - Akbari-Kelachayeh, K.
AU - Silver, J.
AU - Karinni-Abdolrezaee, S.
DO - 10.1002/glia.23533
IS - 1
KW - Spinal Cord Injuries
Receptors, sigma
Spinal Cord
PY - 2019
SN - 0894-1491 1098-1136
SP - 125-145
ST - LAR and PTP sigma receptors are negative regulators of oligodendrogenesis and
oligodendrocyte integrity in spinal cord injury
T2 - GLIA
TI - LAR and PTP sigma receptors are negative regulators of oligodendrogenesis and
oligodendrocyte integrity in spinal cord injury
VL - 67
Y2 - 1
ID - 8431
ER -
TY - JOUR
AB - Due to their potent antibacterial properties, silver nanoparticles (AgNPs)
are widely used in industry and medicine. However, they can cross the brain-blood
barrier, posing a risk to the brain and its functions. In our previous study, we
demonstrated that oral administration of bovine serum albumin (BSA)-coated AgNPs
caused an impairment in spatial memory in a dose-independent manner. In this study,
we evaluated the effects of AgNPs coating material on cognition, spatial memory
functioning, and neurotransmitter levels in rat hippocampus. AgNPs coated with BSA
(AgNPs(BSA)), polyethylene glycol (AgNPs(PEG)), or citrate (AgNPs(Cit)) or silver
ions (Ag+) were orally administered at a dose of 0.5 mg/kg b.w. to male Wistar rats
for a period of 28 days, while the control (Ctrl) rats received 0.2 mL of water.
The acquisition and maintenance of spatial memory related to place avoidance were
assessed using the active allothetic place avoidance task, in which rats from
AgNPs(BSA), AgNPs(PEG), and Ag+ groups performed worse than the Ctrl rats. In the
retrieval test assessing long-term memory, only rats from AgNPs(Cit) and Ctrl
groups showed memory maintenance. The analysis of neurotransmitter levels indicated
that the ratio between serotonin and dopamine concentration was disturbed in the
AgNPs(BSA) rats. Furthermore, treatment with AgNPs or Ag+ resulted in the induction
of peripheral inflammation, which was reflected by the alterations in the levels of
serum inflammatory mediators. In conclusion, depending on the coating material used
for their stabilization, AgNPs induced changes in memory functioning and
concentration of neurotransmitters.
AN - rayyan-553780403
AU - Dziendzikowska, K.
AU - Wesierska, M.
AU - Gromadzka-Ostrowska, J.
AU - Wilczak, J.
AU - Oczkowski, M.
AU - Meczynska-Wielgosz, S.
AU - Kruszewski, M.
DO - 10.3390/ijms222312706
IS - 23
KW - Cognition
Nanoparticles
PY - 2021
SN - 1422-0067
ST - Silver Nanoparticles Impair Cognitive Functions and Modify the Hippocampal
Level of Neurotransmitters in a Coating-Dependent Manner
T2 - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
TI - Silver Nanoparticles Impair Cognitive Functions and Modify the Hippocampal
Level of Neurotransmitters in a Coating-Dependent Manner
VL - 22
Y2 - 12
ID - 8432
ER -
TY - JOUR
AB - Due to undesirable hazardous interactions with biological systems, we
evaluated the effect of silver nanoparticles (AgNPs) intake on oxidative stress and
inflammation. Rats received for 81. days a standard diet (Controls) or a standard
diet plus 500. mg/d/kg BW AgNPs. We assayed plasma lipids, and oxidative stress was
assessed by measuring liver and heart superoxide anion production (O2°-) and liver
malondialdehyde levels (MDA). Antioxidant status was appraised using plasma
paraoxonase activity (PON), plasma antioxidant capacity (PAC) and liver superoxide
dismutase activity (SOD). Liver inflammatory cytokines TNFα and IL-6 levels and
plasma alanine aminotransferase (ALT) were assayed. Compared with Controls, AgNPs
raised cholesterolemia (9.5%), LDL-cholesterol (30%), and lowered triglycerides
(41%). They also increased liver (30%) and cardiac (41%) O2°- production, reduced
PON activity (15%) and raised liver TNFα (9%) and IL-6 (~12%). Plasma ALT activity
rose (12%) after treatment with AgNPs. However, PAC and liver MDA and SOD activity
were unchanged. These features indicate that exposure to 500. mg/d/kg BW of AgNPs
results in liver damage by a dysregulation of lipid metabolism, highlighting liver
and heart as the most sensitive organs to the deleterious effects. Our findings
also demonstrate for the first time the oxidative and inflammatory effects of
dietary AgNPs. © 2013 Elsevier Ltd.
AN - rayyan-553780404
AU - Ebabe Elle, R.
AU - Gaillet, S.
AU - Vidé, J.
AU - Romain, C.
AU - Lauret, C.
AU - Rugani, N.
AU - Cristol, J. P.
AU - Rouanet, J. M.
DO - 10.1016/j.fct.2013.07.071
KW - Collargol
Inflammation
Oxidative stress
Rats
Toxicity
Administration, Oral
Alanine Transaminase
Animals
Antioxidants
Cholesterol
Heart
Hypercholesterolemia
Interleukin-6
Lipid Metabolism
Liver
Liver Diseases
Male
Malondialdehyde
Metal Nanoparticles
Oxidative Stress
Silver
Superoxide Dismutase
Superoxides
Triglycerides
Tumor Necrosis Factor-alpha
aryldialkylphosphatase
interleukin 6
low density lipoprotein cholesterol
malonaldehyde
silver nanoparticle
triacylglycerol
animal experiment
animal model
animal tissue
article
body weight
cardiotoxicity
controlled study
enzyme activity
enzyme blood level
food intake
hypercholesterolemia
inflammation
lipid metabolism
liver level
liver toxicity
male
nonhuman
oxidative stress
rat
PY - 2013
SP - 297-301
ST - Dietary exposure to silver nanoparticles in Sprague-Dawley rats: Effects on
oxidative stress and inflammation
T2 - Food and Chemical Toxicology
TI - Dietary exposure to silver nanoparticles in Sprague-Dawley rats: Effects on
oxidative stress and inflammation
84882949984&doi=10.1016%2fj.fct.2013.07.071&partnerID=40&md5=bb69426426544421ee0aff
ca56758b47
VL - 60
ID - 8433
ER -
TY - JOUR
AB - Leishmaniasis, classified as a neglected tropical disease (NTD), is a
parasitic infection caused by protozoa and transmitted through vectors. It is a
widespread condition found in over 100 countries, known for its destructive nature.
Consequently, there is an increasing need to establish novel approaches for
combating leishmaniasis and developing effective strategies to combat the disease.
In this study, we report an eco-friendly, cost-effective, and biocompatible process
for the preparation of silver nanoparticles (AgNPs) using aqueous extract of Crocus
sativus petals and their antibacterial and anti-leishmanial activities.
Characterization of biosynthesized AgNPs was conducted by UV-vis spectroscopy, TEM,
FTIR, DLS, and FESEM spectroscopy. TEM and FESEM images revealed the existence of
spherical/oval morphology with a size range of 30–70 nm. Additionally, functional
groups associated with the extract were observed, indicating their involvement in
the stabilization and coating of the biologically fabricated AgNPs. Furthermore,
the AgNPs were confirmed through the observation of a surface plasmon response
(SPR) with a peak wavelength of approximately 423 nm. This was accompanied by
noticeable color transformation from transparent to brown, providing further
evidence of the successful formation of AgNPs. Biosynthesized AgNPs from saffron
wastages showed promising anti-leishmanial and antibacterial activities. The AgNPs
displayed a zone of inhibition of 23 mm against Staphylococcus aureus and 10 mm
against Pseudomonas aeruginosa, indicating their potential antibacterial
performance against these microorganisms. The application of an ointment containing
quercetin/saffron-capped silver nanoparticles on mice infected with Leishmania
major resulted in promising outcomes. The treated group exhibited a reduction in
inflammatory responses and an increase in fibroblast activity compared to the
untreated group. These results indicate the potential of this compound to alleviate
inflammation and promote the growth of fibroblasts, which are beneficial for wound
healing and tissue repair. In conclusion, the utilization of biogenically
synthesized silver nanoparticles derived from saffron holds promise as an
alternative therapeutic approach for treating cutaneous leishmaniasis. © 2023, The
Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of
Springer Nature.
AN - rayyan-553780405
AU - Ebrahimzadeh, A.
AU - Karamian, M.
AU - Alemzadeh, E.
AU - Solgi, R.
AU - Mortazavi-Derazkola, S.
DO - 10.1007/s13399-023-04630-x
KW - Anti-leishmanial
Antibacterial
Green synthesis
Saffron wastages
Bacteria
Biocompatibility
Cost effectiveness
Fibroblasts
Mammals
Metal nanoparticles
Morphology
Surface plasmons
Ultraviolet visible spectroscopy
Antibacterials
Classifieds
Crocus sativus
Cutaneous leishmaniasis
Neglected tropical disease
Parasitic infections
Saffron wastage
PY - 2023
ST - Investigation of therapeutic potential in murine cutaneous leishmaniasis and
antibacterial using biosynthesized silver nanoparticles using extract of Crocus
sativus petals
T2 - Biomass Conversion and Biorefinery
TI - Investigation of therapeutic potential in murine cutaneous leishmaniasis and
antibacterial using biosynthesized silver nanoparticles using extract of Crocus
sativus petals
85165259721&doi=10.1007%2fs13399-023-04630-
x&partnerID=40&md5=86b90627dcbe89c25c1786cdc52dacbd
ID - 8434
ER -
TY - JOUR
AB - Drug-loaded nanoparticles are currently gaining attention due to their
improved drug delivery properties. Apocynin, a natural polyphenolic compound, is a
component of many plants. It has many medicinal and pharmacological properties.
Pyrogallol is an anti-psoriatic agent. However, its clinical usage is limited due
to its cumulative and dose-dependent hepatotoxicity. The objective of this study
was to synthesize silver nanoparticles coated with Apocynin (Apo-AgNPs), and
investigate the antioxidant and liver protective effects of Apo-AgNPs on
pyrogallol-induced toxicity in rats. The nanoparticles were characterized and it
was determined that the synthesis technique results in homogeneously dispersed
core–shell Ag structures with spherical forms and an average diameter of 13 nm (6.3
nm). Our results showed that Apo-AgNPs exhibited potent antioxidant and excellent
membrane stability activities in vitro. In rats, Apo-AgNPs (10 and 30 mg/kg)
significantly prevented pyrogallol-induced elevations of alkaline phosphatase,
gamma-glutamyl transferase, creatinine, urea, aspartate aminotransferase, alkaline
aminotransferase, total bilirubin, and decreased blood levels of uric acid.
Moreover, Apo-AgNPs restored the decreased activities of the liver antioxidant
enzymes, including superoxide dismutase and glutathione peroxidase, glutathione
transferase, as well as non-enzyme antioxidant glutathione, as well as
significantly decreased catalase activities which were induced by pyrogallol
treatment. Histological studies indicated that pyrogallol -induced liver damage was
alleviated following Apo-AgNPs treatment in rats. Apo-AgNPs significantly
suppressed the up-regulation of Cyclooxygenase-2 (COX-2), Interleukin 6 (IL-6) and
Nuclear factor-κB (NF-κB) protein expression. These results indicated that Apo-
AgNPs protected the rats from damage via preserving the antioxidant defense
systems, lowering pro-inflammatory cytokines, and expression of COX-2 and NF-κB in
rats. © 2022 Elsevier B.V.
AN - rayyan-553780407
AU - Ekozin, A.
AU - Otuechere, C. A.
AU - Adewuyi, A.
DO - 10.1016/j.cbi.2022.110069
KW - Ampocynin
Hepatic toxicity marker
Nanoparticle
Oxidative stress
Pyrogallol
Acetophenones
Animals
Antioxidants
Chemical and Drug Induced Liver Injury
Cyclooxygenase 2
Lipid Peroxidation
Liver
Metal Nanoparticles
NF-kappa B
Oxidative Stress
Rats
Silver
antioxidant
apocynin
bilirubin
creatinine
cyclooxygenase 2
gamma glutamyltransferase
glutathione
hydrogen peroxide
interleukin 6
malonaldehyde
nitric oxide
pyrogallol
silver nanoparticle
thiobarbituric acid
urea
uric acid
acetophenone derivative
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
blood biochemistry
cervical spine dislocation
controlled study
ferric reducing antioxidant power assay
histology
histopathology
in vitro study
lipid peroxidation
liver protection
liver toxicity
male
nonhuman
oxidative stress
physical chemistry
protein expression
Raman spectrometry
rat
thermogravimetry
thermostability
animal
chemistry
liver
metabolism
toxic hepatitis
PY - 2022
ST - Apocynin loaded silver nanoparticles displays potent in vitro biological
activities and mitigates pyrogallol-induced hepatotoxicity
T2 - Chemico-Biological Interactions
TI - Apocynin loaded silver nanoparticles displays potent in vitro biological
activities and mitigates pyrogallol-induced hepatotoxicity
85135704587&doi=10.1016%2fj.cbi.2022.110069&partnerID=40&md5=83e5e7bacbdbcf607f1726
4512cafb80
VL - 365
ID - 8436
ER -
TY - JOUR
AB - There is serious concern about the potential harmful effects of certain
nanomaterials (NMs), on account of their ability to penetrate cell membranes and
the increased reactivity that results from their increased surface area compared
with bulk chemicals. To assess the safety of NMs, reliable tests are needed. We
have investigated the possible genotoxicity of four representative NMs, derived
from titanium dioxide, zinc oxide, cerium oxide and silver, in two human cell
lines, A549 alveolar epithelial cells and lymphoblastoid TK6 cells. A high-
throughput version of the comet assay was used to measure DNA strand beaks (SBs) as
well as oxidised purines (converted to breaks with the enzyme formamidopyrimidine
DNA glycosylase). In parallel, cytotoxicity was measured with the alamarBlue (R)
assay, and the ability of NM-treated cells to survive was assessed by their colony-
forming efficiency. TiO2 and CeO2 NMs were only slightly cytotoxic by the
alamarBlue r test, and had no long-term effect on colony-forming efficiency.
However, both induced DNA damage at non-cytotoxic concentrations; the damage
decreased from 3 to 24-h exposure, except in the case of CeO2 -treated A549 cells.
ZnO and Ag NMs affected cell survival, and induced high levels of DNA damage at
cytotoxic concentrations. At lower concentrations, there was significant damage,
which tended to persist over 24 h. The implication is that all four reference metal
NMs tested-whether cytotoxic or not-are genotoxic. A full assessment of NM toxicity
should include tests on different cell types, different times of incubation and a
wide range of (especially non-cytotoxic) concentrations; a test for cell viability
should be performed in parallel. Inclusion of Fpg in the comet assay allows
detection of indirect genotoxic effects via oxidative stress.
AN - rayyan-553780408
AU - El Yamani, N.
AU - Collins, A. R.
AU - Runden-Pran, E.
AU - Fjellsbo, L. M.
AU - Shaposhnikov, S.
AU - Zienolddiny, S.
AU - Dusinska, M.
DO - 10.1093/mutage/gew060
IS - 1
KW - Titanium
Mutagenicity Tests
PY - 2017
SN - 0267-8357 1464-3804
SP - 117-126
ST - In vitro genotoxicity testing of four reference metal nanomaterials, titanium
dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment
T2 - MUTAGENESIS
TI - In vitro genotoxicity testing of four reference metal nanomaterials, titanium
dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment
VL - 32
Y2 - 1
ID - 8437
ER -
TY - JOUR
AB - This review summarizes the four processes of wound healing in the human body
(hemostasis, inflammatory, proliferation, and remodeling) and the most current
research on the most important factors affecting cutaneous wound healing and the
underlying cellular and/or molecular pathways. Local factors, including
temperature, oxygenation, and infection, and systemic factors, such as age,
diabetes, sex hormones, genetic components, autoimmune diseases, psychological
stress, smoking and obesity are also addressed. A better understanding of the role
of these factors in wound repair could result in the development of therapeutics
that promote wound healing and resolve affected wounds. Additionally, natural
products obtained from plants and animals are critical targets for the discovery of
novel biologically significant pharmacophores, such as medicines and agrochemicals.
This review outlines the most recent advances in naturally derived targeted
treatment for wound healing. These are plant-derived natural products, insect-
derived natural products, marine-derived natural products, nanomaterial-based
wound-healing therapeutics (metal- and non-metal-based nanoparticles), and natural
product-based nanomedicine to improve the future direction of wound healing.
Natural products extracted from plants and animals have advanced significantly,
particularly in the treatment of wound healing. As a result, the isolation and
extraction of bioactive compounds from a variety of sources can continue to advance
our understanding of wound healing. Undescribed bioactive compounds or unexplored
formulations that could have a role in today's medicinal arsenal may be contained
in the abundance of natural products and natural product derivatives. © 2021
Elsevier Ltd
AN - rayyan-553780409
AU - El-Ashram, S.
AU - El-Samad, L. M.
AU - Basha, A. A.
AU - El Wakil, A.
DO - 10.1016/j.phrs.2021.105749
KW - Insect-derived natural products
Marine-derived natural products
Nanomaterial-based wound-healing therapeutics
Natural product-based nanomedicine
Plant-derived products
Wound healing
Animals
Aquatic Organisms
Biological Products
Humans
Insecta
Nanomedicine
Phytotherapy
Plant Preparations
Skin
Wound Healing
Wounds and Injuries
acaricide
agricultural chemical
apamin
bee venom
catalase
chitosan
chrysin
curcumin
galangin
gelatinase A
glibenclamide
glutathione
gold nanoparticle
graphene
herbaceous agent
hyaluronic acid
hydrogel
hydroxyproline
kaempferol
lipoxygenase
luteolin
metal nanoparticle
methylglyoxal
microRNA
nanocomposite
nanofiber
nanoparticle
natural product
netilmicin
nonmetal
oleic acid
pectin
plasmin
polyvinyl alcohol
quercetin
salicylic acid
saponin
sericin
sex hormone
silver nanoparticle
sunscreen
vasculotropin
biological product
plant medicinal product
Aloe vera
anemia
angiogenesis
antifungal activity
antiphospholipid syndrome
antiviral activity
aquatic environment
autoimmune disease
biocompatibility
biodegradability
cell migration
cell proliferation
cytokine production
cytotoxicity
debridement
depression
diabetes mellitus
diabetic foot
diabetic neuropathy
Down syndrome
drug formulation
drug release
dyspepsia
enzyme activity
episiotomy
epithelization
Escherichia coli
extraction
female
gangrene
gingivitis
glucose blood level
glycosylation
granulation tissue
hemostasis
hermaphrodite
honeybee
human
hyperglycemia
hyperoxia
hypertension
hyperthermia
hypoxia
inflammation
insect
Klinefelter syndrome
male
mental stress
molecularly targeted therapy
mouse
myofibroblast
nanomedicine
nonhuman
obesity
osteolysis
osteomyelitis
oxidative stress
oxygenation
phagocytosis
pharmacophore
quorum sensing
rat
regenerative medicine
Review
sepsis
sickle cell anemia
signal transduction
skin injury
skin irritation
skin water loss
smoking
systemic lupus erythematosus
thrombocyte aggregation
tissue engineering
tissue regeneration
tonsillectomy
vagina flora
virus replication
wound dehiscence
wound healing
wound infection
animal
aquatic species
chemistry
drug effect
injury
metabolism
pathology
phytotherapy
skin
PY - 2021
ST - Naturally-derived targeted therapy for wound healing: Beyond classical
strategies
T2 - Pharmacological Research
TI - Naturally-derived targeted therapy for wound healing: Beyond classical
strategies
85109002351&doi=10.1016%2fj.phrs.2021.105749&partnerID=40&md5=2a59937bec7cec08150f5
3c1d3942a1d
VL - 170
ID - 8438
ER -
TY - JOUR
AB - Bacterial infections are the key cause of death in patients suffering from
burns and diabetic wounds while the use of traditional antibiotics has been growing
steadily. Thus, in the present study, we are trying to introduce a paradigm shift
strategy to improve chronic wound healing of bacterial infection. To that end, we
have biologically synthesized silver nanoparticles (AgNPs) usingArthrospira
sppolysaccharides, and evaluated their antibacterial efficacy with their safety
pattern. Scanning electron micrographs showed spherical AgNPs coated with algal
polysaccharides with an approximate size of 9.7 nm. Treatment ofPseudomonas
aeruginosawith the AgNPs (0.5-1 mu g/mL) resulted in a significant disruption inP.
aeruginosaouter membrane, reduction in biofilm formation, and a significant
decrease of production of alginate and pyocyanin along with a concentration-
dependent reduction in beta-lactamase activity. In addition, at thein vivolevel,
AgNPs displayed substantial activity to controlP. aeruginosainfections in rat skin
wounds with significant reduction in in COX-2 enzyme in both rat skin homogenate
and serum samples. Furthermore, AgNPs facilitated wound curative in theP.
aeruginosainfected model by reducing the hemorrhagic areas number and the
infiltrated inflammatory cells. Taken all together, these biogenic nanoparticles
showed unique properties in controlling bacterial wound infections and improving
the healing process of damaged tissues via its direct and indirect effects.
AN - rayyan-553780410
AU - El-Deeb, N. M.
AU - Abo-Eleneen, M. A.
AU - Al-Madboly, L. A.
AU - Sharaf, M. M.
AU - Othman, S. S.
AU - Ibrahim, O. M.
AU - Mubarak, M. S.
DO - 10.3389/fbioe.2020.00643
PY - 2020
SN - 2296-4185
ST - Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles:
Immunomodulatory and Antibacterial Potentialities Against ResistantPseudomonas
aeruginosa
T2 - FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
TI - Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles:
Immunomodulatory and Antibacterial Potentialities Against ResistantPseudomonas
aeruginosa
VL - 8
Y2 - 7 y3 - 21
ID - 8439
ER -
TY - JOUR
AB - To find potential alternatives for certain conventional antibiotics, we
investigated the effects of silver nanoparticles (AgNPs) synthesized using Moringa
oleifera extract on serum biochemistry, immunological, inflammatory, oxidative, and
histological alterations, and DNA damage in Oreochromis niloticus infected with
Aeromonas hydrophila. For determining the concentration of AgNPs, 110 fish were
used; 11 groups were challenged with Aeromonas hydrophila (each group = 10 fish)
and exposed to different concentrations of AgNPs (0, 0.4, 0.8, 1.2, 1.6, 2, 2.4,
2.8, 3.2, 3.6, and 4.0 mg/L) as immersion. Another 360 fish were categorized into
eight groups: G1 was non-infected with Aeromonas hydrophila and treated with 0 mg/L
AgNPs, G2 was infected and treated with 0 mg/L AgNPs, G3, G4, and G5 were non-
infected and treated with 0.4, 0.8, and 1.2 mg/L AgNPs, respectively. G6, G7, and
G8 were infected and treated with 0.4, 0.8, and 1.2 mg/L AgNPs, respectively, for
seven days. Infection with Aeromonas hydrophila significantly altered the health
and reduced lysozyme, hepatic CAT, and hepatic SOD activity, and IgM, complement 3,
total protein, albumin, globulin, serum nitric oxide, and IL-10 levels, with a
significant increase in the activity of ALT, AST, LDH, and levels of urea,
creatinine, bilirubin, hepatic MDA, and inflammatory biomarkers (IL-1β and IL-6).
Moreover, the infection produced a prominent genotoxic effect and impaired the
architecture of hepatic, renal, splenic, and gill tissues with a strong expression
of NF-kB. Fish exposed to different treatments of AgNPs showed improvement in these
markers with a significant reduction in the mortality rate. Interestingly, the
submersion of infected fish in 0.8 mg/L AgNPs produced the best result. These
results showed that green biosynthesis of AgNPs is non-cytotoxic and provides an
alternative antimicrobial compound against hepatotoxic, splenotoxic, nephrotoxic,
genotoxic, and immunosuppressive impacts of Aeromonas hydrophila in Nile tilapia. ©
2021 Elsevier B.V.
AN - rayyan-553780413
AU - El-Houseiny, W.
AU - Mansour, M. F.
AU - Mohamed, W. A. M.
AU - Al-Gabri, N. A.
AU - El-Sayed, A. A.
AU - Altohamy, D. E.
AU - Ibrahim, R. E.
DO - 10.1016/j.aquaculture.2021.736430
KW - Aeromonas hydrophila
Green synthesis
Immunity
Moringa oleifera
Nile tilapia
Nuclear factor-kappa B
Oreochromis niloticus
antibiotics
apoptosis
bacterium
biochemistry
cichlid
concentration (composition)
genotoxicity
immune response
induced response
nanoparticle
oxidative stress
pollution effect
silver
Oxidative Stress
PY - 2021
ST - Silver nanoparticles mitigate Aeromonas hydrophila-induced immune
suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis
niloticus
T2 - Aquaculture
TI - Silver nanoparticles mitigate Aeromonas hydrophila-induced immune
suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis
niloticus
85100062381&doi=10.1016%2fj.aquaculture.2021.736430&partnerID=40&md5=cb9e23de371546
327b822d89ec1e0404
VL - 535
ID - 8442
ER -
TY - JOUR
AB - A superfusion apparatus (SA) was developed to maintain isolated human corneas
ex vivo under conditions which mimic the natural eye environment in vivo, including
controlled temperature, tear flow and intraocular pressure. The SA was designed,
developed and tested for use in ophthalmic pre-clinical research and to test new
pharmaceutical formulations. Corneas undergo an equilibration process in the new
physiological environment for one day. The test was then initiated by the
application of the test substance, incubation, and temporal assessment of corneal
damage using various parameters. The effects of mild and severe irritant
concentrations of NaOH (2% and 8%, respectively) on corneal opacity, swelling and
epithelial integrity were studied, and the inflammatory status assessed using F4/80
and MPO as macrophages and neutrophils markers, respectively. The SA was then used
to test new artificial tear formulations supplemented with silver ions as an active
constituent, showing different degrees of inflammatory responses as indicated by
the migration of MPO and F4/80 positive cells towards the epithelium. The human
cornea superfusion apparatus was proposed as a model for acute eye irritation
research.
AN - rayyan-553782360
AU - Elbadawy, H. M.
AU - Salvalaio, G.
AU - Parekh, M.
AU - Ruzza, A.
AU - Baruzzo, M.
AU - Cagini, C.
AU - Ponzin, D.
AU - Ferrari, S.
DO - 10.1016/j.tiv.2015.06.022
IS - 7
J2 - Toxicol In Vitro
KW - *Animal Testing Alternatives
Antigens, Differentiation/metabolism
Ascorbic Acid/toxicity
Caustics/*toxicity
Cornea/*drug effects/pathology
Corneal Opacity
Humans
In Vitro Techniques
Irritants/*toxicity
Lubricant Eye Drops/toxicity
Ophthalmic Solutions
Peroxidase/metabolism
Silver Nitrate/toxicity
Sodium Hydroxide/*toxicity
Humanities
Humanism
LA - eng
N1 - International Centre for Ocular Physiopathology, The Eye Bank Foundation of
Veneto, Via Paccagnella 11, Padiglione Rama, Venice 30174, Italy. Electronic
address: hossein.elbadawy@hotmail.com.; International Centre for Ocular
Physiopathology, The Eye Bank Foundation of Veneto, Via Paccagnella 11, Padiglione
Rama, Venice 30174, Italy.; International Centre for Ocular Physiopathology, The
Eye Bank Foundation of Veneto, Via Paccagnella 11, Padiglione Rama, Venice 30174,
Italy.; International Centre for Ocular Physiopathology, The Eye Bank Foundation of
Veneto, Via Paccagnella 11, Padiglione Rama, Venice 30174, Italy.; International
Centre for Ocular Physiopathology, The Eye Bank Foundation of Veneto, Via
Paccagnella 11, Padiglione Rama, Venice 30174, Italy.; The Department of
Ophthalmology, Perugia General Hospital, University of Perugia, Perugia 06123,
Italy.; International Centre for Ocular Physiopathology, The Eye Bank Foundation of
Veneto, Via Paccagnella 11, Padiglione Rama, Venice 30174, Italy.; International
Centre for Ocular Physiopathology, The Eye Bank Foundation of Veneto, Via
Paccagnella 11, Padiglione Rama, Venice 30174, Italy.
PY - 2015
SP - 1619-27
ST - A superfusion apparatus for ex vivo human eye irritation investigations
T2 - Toxicology in vitro : an international journal published in association with
BIBRA
TI - A superfusion apparatus for ex vivo human eye irritation investigations
VL - 29
Y2 - 10
ID - 10267
ER -
TY - JOUR
AB - For the next-generation risk assessment (NGRA) of chemicals and
nanomaterials, new approach methodologies (NAMs) are needed for hazard assessment
in compliance with the 3R's to reduce, replace and refine animal experiments. This
study aimed to establish and characterize an advanced respiratory model consisting
of human epithelial bronchial BEAS-2B cells cultivated at the air-liquid interface
(ALI), both as monocultures and in cocultures with human endothelial EA.hy926
cells. The performance of the bronchial models was compared to a commonly used
alveolar model consisting of A549 in monoculture and in coculture with EA.hy926
cells. The cells were exposed at the ALI to nanosilver (NM-300K) in the VITROCELL
(R) Cloud. After 24 h, cellular viability (alamarBlue assay), inflammatory response
(enzyme-linked immunosorbent assay), DNA damage (enzyme-modified comet assay), and
chromosomal damage (cytokinesis-block micronucleus assay) were measured.
Cytotoxicity and genotoxicity induced by NM-300K were dependent on both the cell
types and model, where BEAS-2B in monocultures had the highest sensitivity in terms
of cell viability and DNA strand breaks. This study indicates that the four ALI
lung models have different sensitivities to NM-300K exposure and brings important
knowledge for the further development of advanced 3D respiratory in vitro models
for the most reliable human hazard assessment based on NAMs.
AN - rayyan-553780418
AU - Elje, E.
AU - Mariussen, E.
AU - McFadden, E.
AU - Dusinska, M.
AU - Runden-Pran, E.
DO - 10.3390/nano13030407
IS - 3
KW - Proportional Hazards Models
ADP Ribose Transferases
PY - 2023
SN - 2079-4991
ST - Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture
Models for Hazard Assessment of Nanomaterials
T2 - NANOMATERIALS
TI - Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture
Models for Hazard Assessment of Nanomaterials
VL - 13
Y2 - 2
ID - 8447
ER -
TY - JOUR
AB - Thioacetamide (TAA) is one of the common fungicidal agents that induce liver
injury varying from inflammation, necrosis, and fibrosis to cirrhosis. Many recent
studies reported the beneficial effect of probiotics and silymarin on
hepatotoxicity regardless the causative agents. Therefore, the present study aimed
to evaluate the ameliorative role of probiotics and/or silymarin on TAA induced
hepatotoxicity in rats via histological, and immunohistochemical methods. Twenty
five male albino rats were used for this experiment and were divided into five
groups (n=5 rats/group); group I acts as negative control, group II was orally
administrated distilled water for six weeks, then injected with TAA (200 mg/kg
b.wt./ 5 ml physiological saline/ I.P.) twice a week for another six weeks, group
III was treated with probiotics at a dose of 135 mg/ kg b.wt. orally in drinking
water daily for six weeks, then injected with TAA (dosage of group II), twice
weekly for another six weeks, group IV was treated with silymarin at a dose of 200
mg/ kg b.wt orally 4 times per week for six weeks, then injected with TAA (dosage
of group II), twice weekly for another six weeks and group V was treated with
combination of both probiotics and silymarin, at the same dosage in groups III and
IV respectively then injected with TAA (dosage of group II), twice weekly for
another six weeks. Histologically, TAA induced hepatocytes degeneration,
inflammatory cells infiltration, and pseudolobular parenchyma as well as, high
apoptosis and low proliferation rates that were proved by immunohistochemical
staining for caspase 3 and ki-67 respectively. Probiotics and/or silymarin improved
the histological feature of hepatocytes, reduced apoptosis and stimulated
proliferation. Based on these results, we concluded that the use of probiotics and
silymarin combination ameliorates the hepatotoxic effect of TAA in rats more than
the use of probiotics or silymarin alone. La tioacetamida (TAA) es uno de los
agentes fungicidas más comunes que inducen lesiones hepáticas que varían desde
inflamación, necrosis y fibrosis hasta cirrosis. Muchos estudios recientes
informaron el efecto beneficioso de los probióticos y la silimarina sobre la
hepatotoxicidad independientemente de los agentes causantes. Por lo tanto, el
presente estudio tuvo como objetivo evaluar el papel paliativo de los probióticos y
/ o silimarina en la hepatotoxicidad inducida por TAA en ratas a través de métodos
histológicos e inmunohistoquímicos. Para este experimento se usaron veinticinco
ratas albinas y se dividieron en cinco grupos (n = 5 ratas / grupo); el grupo I se
usó como control negativo; en el grupo II se administró por vía oral agua destilada
durante seis semanas y luego se inyectó TAA (200 mg / kg b.wt./ 5 ml solución
salina fisiológica / IP) dos veces por semana durante otras seis semanas; el grupo
III se trató con probióticos, dosis diaria de 135 mg / kg b.wt. por vía oral en
agua potable, durante seis semanas y luego fue inyectado con TAA (dosis del grupo
II), dos veces por semana durante otras seis semanas; el grupo IV se trató con
silimarina, con una dosis de 200 mg / kg b.wt por vía oral 4 veces por semana
durante seis semanas, luego se inyectó TAA (dosificación del grupo II), dos veces
por semana durante otras seis semanas; y el grupo V, se trató con una combinación
de ambos probióticos y silimarina con la misma dosis que en los grupos III y IV,
respectivamente, luego fueron inyectados con TAA (dosificación del grupo II), dos
veces por semana durante otras seis semanas. Histológicamente, la TAA indujo la
degeneración de los hepatocitos, la infiltración de células inflamatorias y el
parénquima pseudolobular, así como también una apoptosis alta y tasas de
proliferación bajas que se probaron mediante tinción inmunohistoquímica para
caspasa 3 y ki-67, respectivamente. Los probióticos y / o la silimarina mejoraron
la característica histológica de los hepatocitos, redujeron la apoptosis y
estimularon la proliferación. En base a estos resultados, conc uimos que el uso de
la combinación de probióticos y silimarina mejora el efecto hepatotóxico del TAA en
ratas más que el uso de probióticos o silimarina individualmente.
AN - rayyan-553780422
AU - Emam, Mahmoud Abdelghaffar
AU - Farouk, Sameh Mohamed
AU - Abdo, Mohamed
DO - 10.4067/S0717-95022018000200661
IS - 2
KW - Histology
Histología, Inmunohistoquímica
Hígado
Liver
Probiotics
Probióticos
Silimarina
Silymarin
Thioacetamide
Tioacetamida
Rats
LA - en
PY - 2018
SN - 0717-9367
SP - 661-669
ST - The ameliorative potential of probiotics and/or silymarin on thioacetamide
induced hepatotoxicity in rats: histological and immunohistochemical study
TI - The ameliorative potential of probiotics and/or silymarin on thioacetamide
induced hepatotoxicity in rats: histological and immunohistochemical study
95022018000200661
VL - 36
Y2 - 6 y3 - 1
ID - 8451
ER -
TY - JOUR
AB - OBJECTIVE: To study the localisation of treponemes and to analyse the
inflammatory infiltrate in biopsy specimens from patients with primary or secondary
syphilis, or early infectious yaws. MATERIALS AND METHODS: Skin biopsies
originating from human lesions of primary (29x) or secondary (15x) syphilis
(Rotterdam), or early yaws (18x) (West Sumatra) were studied. Different
histochemical and immunohistochemical detection methods were used in this study.
RESULTS AND CONCLUSION: The histochemical silver staining method according to
Steiner revealed the presence of T. pallidum in all cases of primary syphilis
studied. In 10 out of 14 cases of secondary syphilis, treponemes were demonstrated.
With an immunofluorescence staining technique (IF) using anti-T. pallidum antiserum
raised in rabbits (a-Tp), T. pallidum was demonstrated in 28 out of 29 cases of
primary syphilis, and in 14 out of 14 studied cases of secondary syphilis. The
silver staining method and IF showed identical localisations of T. pallidum (mainly
in the dermal-epidermal junction zone or throughout the dermis). Using a-Tp
antiserum in the indirect immunofluorescence technique, T. pertenue could be
demonstrated in the dermis more often than with Steiner silver staining. However,
epidermotropism of T. pertenue in yaws specimens was remarkable, compared with more
mesodermotropism of T. pallidum; numbers of T. pertenue in the dermis were limited
in all specimens. The dermal inflammatory infiltrate in primary and secondary
syphilis was composed mainly of lymphocytes and plasma cells. In most cases more T
(CD3 positive) cells than B (CD22 positive) cells were present. Regarding T cell
subpopulations, in primary syphilis, T helper/inducer (CD4 positive) cells
predominated in 86% of cases. In secondary syphilitic lesions, numbers of T
helper/inducer cells were less frequent than or equal to T-suppressor/cytotoxic
(CD8 positive) cells in 60% of cases. Remarkably, in yaws specimens the
inflammatory infiltrate consisted mainly of IgG, but also IgA and IgM producing
plasma cells. T or B lymphocytes were scarce, which is in sharp contrast with
findings in syphilitic lesions.
AN - rayyan-553782367
AU - Engelkens, H. J.
AU - ten Kate, F. J.
AU - Judanarso, J.
AU - Vuzevski, V. D.
AU - van Lier, J. B.
AU - Godschalk, J. C.
AU - van der Sluis, J. J.
AU - Stolz, E.
DO - 10.1136/sti.69.2.102
IS - 2
J2 - Genitourin Med
KW - Adolescent
Antibodies, Bacterial/analysis
Child
Child, Preschool
Female
Humans
Infant
Male
Silver Staining
Skin/*microbiology
Syphilis, Cutaneous/immunology/*microbiology
Treponema pallidum/immunology/*isolation & purification
Yaws/immunology/*microbiology
Biopsy
Skin
LA - eng
N1 - Department of Dermatology and Venereology, University Hospital Rotterdam-
Dijkzigt, The Netherlands.
PY - 1993
SN - 0266-4348 (Print)
SP - 102-7
ST - The localisation of treponemes and characterisation of the inflammatory
infiltrate in skin biopsies from patients with primary or secondary syphilis, or
early infectious yaws
T2 - Genitourinary medicine
TI - The localisation of treponemes and characterisation of the inflammatory
infiltrate in skin biopsies from patients with primary or secondary syphilis, or
early infectious yaws
VL - 69
Y2 - 4
ID - 10274
ER -
TY - JOUR
AB - The immune system contributes to maintaining the body's functional integrity
through its two main functions: recognizing and destroying foreign external agents
(invading microorganisms) and identifying and eliminating senescent cells and
damaged or abnormal endogenous entities (such as cellular debris or
misfolded/degraded proteins). Accordingly, the immune system can detect molecular
and cellular structures with a spatial resolution of a few nm, which allows for
detecting molecular patterns expressed in a great variety of pathogens, including
viral and bacterial proteins and bacterial nucleic acid sequences. Such patterns
are also expressed in abnormal cells. In this context, it is expected that
nanostructured materials in the size range of proteins, protein aggregates, and
viruses with different molecular coatings can engage in a sophisticated interaction
with the immune system. Nanoparticles can be recognized or passed undetected by the
immune system. Once detected, they can be tolerated or induce defensive
(inflammatory) or anti-inflammatory responses. This paper describes the different
modes of interaction between nanoparticles, especially inorganic nanoparticles, and
the immune system, especially the innate immune system. This perspective should
help to propose a set of selection rules for nanosafety-by-design and medical
nanoparticle design.
AN - rayyan-553780423
AU - Ernst, L. M.
AU - Casals, E.
AU - Italiani, P.
AU - Boraschi, D.
AU - Puntes, V.
DO - 10.3390/nano11112991
IS - 11
KW - Immune System
PY - 2021
SN - 2079-4991
ST - The Interactions between Nanoparticles and the Innate Immune System from a
Nanotechnologist Perspective
T2 - NANOMATERIALS
TI - The Interactions between Nanoparticles and the Innate Immune System from a
Nanotechnologist Perspective
VL - 11
Y2 - 11
ID - 8452
ER -
TY - JOUR
AB - Zinc oxide (ZnO) nanoparticles (NPs) have received considerable attention in
the medical field because of their antibacterial properties, primarily for killing
and reducing the activity of numerous microorganisms. The purpose of this study was
to determine whether surface-modified ZnO NPs exhibit different properties compared
with unmodified ZnO. The antimicrobial and cytotoxic properties of modified ZnO NPs
as well as their effects on inflammatory cytokine production were evaluated. ZnO
NPs were prepared using a wet chemical method. Then, the surfaces of these NPs were
modified using 3-aminopropyltriethoxysilane (APTES) and dimethyl sulfoxide (DMSO)
as modifying agents via a chemical hydrolysis method. According to infrared
spectroscopy analysis (FTIR), the structure of the ZnO remained unchanged after
modification. Antibacterial assays demonstrated that APTES modification is more
effective at inducing an antimicrobial effect against Gram-negative bacteria than
against Gram-positive bacteria. Cytotoxicity studies showed that cell viability was
dose-dependent; moreover, pristine and APTES-modified ZnO exhibited low
cytotoxicity, whereas DMSO-modified ZnO exhibited toxicity even at a low NP
concentration. An investigation of inflammatory cytokine production demonstrated
that the extent of stimulation was related to the ZnO NP concentration but not to
the surface modification, except for IFN-gamma and IL-10, which were not detected
even at high NP concentrations. (C) 2016 Elsevier Ltd. All rights reserved.
AN - rayyan-553780425
AU - Esparza-Gonzalez, S. C.
AU - Sanchez-Valdes, S.
AU - Ramirez-Barron, S. N.
AU - Loera-Arias, M. J.
AU - Bernal, J.
AU - Melendez-Ortiz, H. I.
AU - Betancourt-Galindo, R.
DO - 10.1016/j.tiv.2016.09.020
KW - Cytotoxins
PY - 2016
SN - 0887-2333
SP - 134-141
ST - Effects of different surface modifying agents on the cytotoxic and
antimicrobial properties of ZnO nanoparticles
TI - Effects of different surface modifying agents on the cytotoxic and
antimicrobial properties of ZnO nanoparticles
VL - 37
Y2 - 12
ID - 8454
ER -
TY - JOUR
AB - Background: Emerging data implicate nicotinamide phosphoribosyl transferase
(NAMPT) in the pathogenesis of cancer and inflammation. NAMPT inhibitors have
proven beneficial in inflammatory animal models of arthritis and endotoxic shock as
well as in autoimmune encephalitis. Given the role of inflammatory responses in
spinal cord injury (SCI), the effect of NAMPT inhibitors was examined in this
setting.Methods: We investigated the effects of the NAMPT inhibitor FK866 in an
experimental compression model of SCI.Results: Twenty-four hr following induction
of SCI, a significant functional deficit accompanied widespread edema,
demyelination, neuron loss and a substantial increase in TNF-α, IL-1β, PAR, NAMPT,
Bax, MPO activity, NF-κB activation, astrogliosis and microglial activation was
observed. Meanwhile, the expression of neurotrophins BDNF, GDNF, NT3 and anti-
apoptotic Bcl-2 decreased significantly. Treatment with FK866 (10 mg/kg), the best
known and characterized NAMPT inhibitor, at 1 h and 6 h after SCI rescued motor
function, preserved perilesional gray and white matter, restored anti-apoptotic and
neurotrophic factors, prevented the activation of neutrophils, microglia and
astrocytes and inhibited the elevation of NAMPT, PAR, TNF-α, IL-1β, Bax expression
and NF-κB activity.We show for the first time that FK866, a specific inhibitor of
NAMPT, administered after SCI, is capable of reducing the secondary inflammatory
injury and partly reduce permanent damage. We also show that NAMPT protein levels
are increased upon SCI in the perilesional area which can be corrected by
administration of FK866.Conclusions: Our findings suggest that the inflammatory
component associated to SCI is the primary target of these inhibitors. © 2012
Esposito et al; licensee BioMed Central Ltd.
AN - rayyan-553780426
AU - Esposito, E.
AU - Impellizzeri, D.
AU - Mazzon, E.
AU - Fakhfouri, G.
AU - Rahimian, R.
AU - Travelli, C.
AU - Tron, G. C.
AU - Genazzani, A. A.
AU - Cuzzocrea, S.
DO - 10.1186/1742-2094-9-66
KW - Apoptosis
Cytokines
Inflammation
NAMPT inhibitor
Neurotrophic factors
Spinal cord injury
Acrylamides
Alcohol Oxidoreductases
Animals
Enzyme Inhibitors
In Situ Nick-End Labeling
Laminectomy
Male
Mice
Movement Disorders
Nerve Growth Factors
Nerve Tissue Proteins
Neutrophil Infiltration
NF-kappa B
Nicotinamide Phosphoribosyltransferase
Peroxidase
Phosphorylation
Piperidines
Silver Staining
Spinal Cord
Spinal Cord Injuries
Time Factors
brain derived neurotrophic factor
daporinad
interleukin 1beta
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
nicotinamide phosphoribosyltransferase
protein Bax
serine
synaptotagmin I
acrylamide derivative
alcohol dehydrogenase
cytokine
enzyme inhibitor
N (4 (1 benzoylpiperidin 4 yl)butyl) 3 (pyridin 3 yl)acrylamide
N-(4-(1-benzoylpiperidin-4-yl)butyl)-3-(pyridin-3-yl)acrylamide
nerve growth factor
nerve protein
nicotinamide phosphoribosyltransferase, mouse
peroxidase
phenylacetaldehyde reductase
piperidine derivative
animal experiment
animal model
apoptosis
article
astrocyte
cell activation
controlled study
cytokine production
demyelination
disease severity
drug efficacy
enzyme activity
male
microglia
motor activity
motor performance
nonhuman
protein expression
spinal cord injury
treatment response
animal
disease model
drug effect
immunology
laminectomy
metabolism
motor dysfunction
mouse
nick end labeling
pathology
phosphorylation
silver staining
spinal cord
time
ultrastructure
PY - 2012
ST - The NAMPT inhibitor FK866 reverts the damage in spinal cord injury
T2 - Journal of Neuroinflammation
TI - The NAMPT inhibitor FK866 reverts the damage in spinal cord injury
84859482714&doi=10.1186%2f1742-2094-9-
66&partnerID=40&md5=915f3fbcf36a49801be560b6ee2eb188
VL - 9
ID - 8455
ER -
TY - JOUR
AB - Resistance to therapy-mediated apoptosis in inflammatory breast cancer, an
aggressive and distinct subtype of breast cancer, was recently attributed to
increased superoxide dismutase (SOD) expression, glutathione (GSH) content, and
decreased accumulation of reactive species. In this study, we demonstrate the
unique ability of two Mn(III) N-substituted pyridylporphyrin (MnP)-based SOD mimics
(MnTE-2-PyP5+ and MnTnBuOE-2-PyP5+) to catalyze oxidation of ascorbate, leading to
the production of excessive levels of peroxide, and in turn cell death. The
accumulation of peroxide, as a consequence of MnP+ ascorbate treatment, was fully
reversed by the administration of exogenous catalase, showing that hydrogen
peroxide is essential for cell death. Cell death as a consequence of the action of
MnP + ascorbate corresponded to decreases in GSH levels, prosurvival signaling (p-
NF-kappa B, p-ERK1/2), and in expression of X-linked inhibitor of apoptosis
protein, the most potent caspase inhibitor. Although markers of classical apoptosis
were observed, including PARP cleavage and annexin V staining, administration of a
pan-caspase inhibitor, Q-VD-OPh, did not reverse the observed cytotoxicity. MnP +
ascorbate-treated cells showed nuclear translocation of apoptosis-inducing factor,
suggesting the possibility of a mechanism of caspase-independent cell death.
Pharmacological ascorbate has already shown promise in recently completed phase I
clinical trials, in which its oxidation and subsequent peroxide formation was
catalyzed by endogenous metalloproteihs. The catalysis of ascorbate oxidation by an
optimized metalbased catalyst (such as MnP) carries a large therapeutic potential
as an anticancer agent by itself or in combination with other modalities such as
radio- and chemotherapy. (C) 2013 Elsevier Inc. All rights reserved.
AN - rayyan-553780427
AU - Evans, M. K.
AU - Tovmasyan, A.
AU - Batinic-Haberle, I.
AU - Devi, G. R.
DO - 10.1016/j.freeradbiomed.2013.11.031
PY - 2014
SN - 0891-5849 1873-4596
SP - 302-314
ST - Mn porphyrin in combination with ascorbate acts as a pro-oxidant and mediates
caspase-independent cancer cell death
T2 - FREE RADICAL BIOLOGY AND MEDICINE
TI - Mn porphyrin in combination with ascorbate acts as a pro-oxidant and mediates
caspase-independent cancer cell death
VL - 68
Y2 - 3
ID - 8456
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely applied in various aspects of life.
However, recent studies reported their potential toxicity both on environment and
human health. The present study aimed to unravel the underlying molecular
mechanisms involved in AgNPs-induced brain toxicity. Moreover, chemopreventive
effect of tranilast, an analogue of tryptophan metabolite and a mast cell membrane
stabilizer was evaluated. Thirty Sprague Dawley rats were enrolled equally into
Normal control group, AgNPs-intoxicated group (50 mg/kg, 3 times/week) and
tranilast (300 mg/kg, 3 times/week)+AgNPs group. AgNPs administration triggered
brain oxidative stress as depicted by reduced Nrf-2 expression, decreased TAC and
GSH as well as upregulated brain lipid peroxidation. The apparent brain oxidative
damage was accompanied by elevated levels of inflammatory cytokines (IL-1β, IL-6
and TNF-α). Moreover, brain levels of TLR4, NLRP3 and caspase-1 were up-regulated.
Additionally, histological study indicated marked cellular injury in cerebrum and
cerebellum specimens. This was concomitant with elevated serum CK activity and CK-
BB level. On the other hand, tanilast administration remarkably alleviated AgNPs-
induced brain toxicity. The present study shed the light on implication of
TLR4/NLRP3 axis and NrF2 in AgNPs-induced brain toxicity. In addition, it explored
the potential protective effect of tranilast on AgNPs-induced brain injury via
antioxidant and anti-inflammatory efficacies. © 2020 Elsevier B.V.
AN - rayyan-553780428
AU - Fahmy, E. K.
AU - El-Sherbiny, M.
AU - Said, E.
AU - Elkattawy, H. A.
AU - Qushawy, M.
AU - Elsherbiny, N.
DO - 10.1016/j.neuro.2020.12.008
KW - Cerebral toxicity
NLRP3
Nrf-2
Tranilast
Animals
Caspase 1
Cerebrum
Creatine Kinase
Creatine Kinase, BB Form
Interleukin-1beta
Interleukin-6
Metal Nanoparticles
Neuroprotective Agents
NF-E2-Related Factor 2
NLR Family, Pyrin Domain-Containing 3 Protein
ortho-Aminobenzoates
Oxidative Stress
Rats
Silver Compounds
caspase 3
CD68 antigen
creatine kinase
creatine kinase BB
cryopyrin
glutathione
interleukin 1beta
interleukin 1beta converting enzyme
interleukin 6
malonaldehyde
silver nanoparticle
tranilast
transcription factor Nrf2
tryptophan
anthranilic acid derivative
metal nanoparticle
neuroprotective agent
Nlrp3 protein, rat
silver derivative
Tlr4 protein, rat
adult
animal cell
animal experiment
animal model
animal tissue
Article
brain homogenate
brain injury
brain tissue
brain toxicity
cell damage
cerebellum
chemoprophylaxis
choroid plexus
controlled study
drug mechanism
enzyme activity
histopathology
in vivo study
lipid peroxidation
nerve cell necrosis
nonhuman
oxidative stress
priority journal
protein cleavage
protein expression
rat
Sprague Dawley rat
upregulation
animal
blood
drug effect
metabolism
pathology
PY - 2021
SP - 167-176
ST - Tranilast ameliorated subchronic silver nanoparticles-induced cerebral
toxicity in rats: Effect on TLR4/NLRP3 and Nrf-2
T2 - NeuroToxicology
TI - Tranilast ameliorated subchronic silver nanoparticles-induced cerebral
toxicity in rats: Effect on TLR4/NLRP3 and Nrf-2
85098147543&doi=10.1016%2fj.neuro.2020.12.008&partnerID=40&md5=8d5801547e67e02df625
311cb63adaae
VL - 82
ID - 8457
ER -
TY - JOUR
AB - We presented a low-cost, eco-friendly, and efficient bacterium-mediated
synthesis of zinc oxide nanoparticles (ZnO-NPs) utilizing Paraclostridium
benzoelyticum strain 5610 as a capping and reducing agent. Scanning electron
microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy-
dispersive X-ray, and UV-vis spectroscopy were used to physiochemically
characterize the biosynthesized ZnO-NPs. A major narrow peak at 441 nm was observed
using UV-visible spectroscopy, verifying the presence of nanoparticles. According
to SEM and TEM studies, the average dimensions of ZnO-NPs was 50 nm. The crystal
size of 48.22 nm was determined by XRD analysis. FTIR analysis confirmed the
presence of various reducing metabolites on the surface of ZnO-NPs. The synthesized
nanoparticles were investigated for biological activity against Helicobacter suis,
Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis.
Helicobacter suis was the most vulnerable strain, with an inhibitory zone of 19.53
+/- 0.62 mm at 5 mg/mL dosage. The anti-inflammatory and the findings of the rat
paw edema experiments revealed that the bacterium-mediated ZnO-NPs had a strong
inhibitory action. In the arthritis model, the solution of ZnO-NPs showed 87.62 +/-
0.12% inhibitory effect of edema after 21 days when linked with that of the
standard drug. In the antidiabetic assay, ZnO-NPs sharply reduced glucose level in
STZ-induced diabetic mice. In this study, the particle biocompatibility by human
red blood cells was also determined. Keeping in view the biological importance of
ZnO-NPs, we may readily get the conclusion that Paraclostridium benzoelyticum
strain 5610-mediated ZnO-NPs will be a prospective antidiabetic, antibacterial,
antiarthritic, and anti-inflammatory agent in vivo experimental models and can be
used as a potent antidiabetic drug.
AN - rayyan-553780429
AU - Faisal, S.
AU - Rizwan, M.
AU - Ullah, R.
AU - Alotaibi, A.
AU - Khattak, A.
AU - Bibi, N.
AU - Idrees, M.
DO - 10.1155/2022/5994033
KW - Zinc
PY - 2022
SN - 1942-0900 1942-0994
ST - Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and
Their In Vivo Multiple Biological Applications
T2 - OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
TI - Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and
Their In Vivo Multiple Biological Applications
VL - 2022
Y2 - 5 y3 - 5
ID - 8458
ER -
TY - JOUR
AB - The current study aims to utilize the bacteria Paraclostridium benzoelyticum
strain 5610 to synthesize bio-genic silver nanoparticles (AgNPs). Biogenic AgNPs
were thoroughly examined using various characterization techniques such as UV-
spectroscopy, XRD, FTIR, SEM, and EDX. Synthesis of AgNPs was confirmed by UV–vis
analysis resulting in absorption peak at 448.31 nm wavelength. The SEM analysis
indicated the morphological characteristics and size of AgNPs which was 25.29 nm.
The face centered cubic (FCC) crystallographic structure was confirmed by XRD.
Furthermore, FTIR study affirmed the capping of AgNPs by different compounds found
in biomass of the Paraclostridium benzoelyticum strain 5610. Later, EDX was used to
determine the elemental composition with respective concentration and distribution.
Additionally, in the current study the antibacterial, anti-inflammatory,
antioxidant, anti-aging, and anti-cancer ability of AgNPs was assessed. The
antibacterial activity of AgNPs was tested against four distinct sinusitis
pathogens: Haemophilus in-fluenza, Streptococcus pyogenes, Moraxella catarrhalis
and Streptococcus pneumonia. AgNPs shows significant inhibition zone against
Streptococcus pyogenes 16.64 ± 0.35 followed by 14.32 ± 071 for Moraxella
catarrhalis. Similarly, the antioxidant potential was found maximum (68.37 ± 0.55%)
at 400 μg/mL and decrease (5.48 ± 0.65%) at 25 μg/mL, hence the significant
antioxidant ability was observed. Furthermore, anti-inflammatory activity of AgNPs
shows the strongest inhibitory action (42.68 ± 0.62%) for 15-LOX with lowest
inhibition activity for COX-2 (13.16 ± 0.46%). AgNPs have been shown to exhibit
significant inhibitory actions against the enzyme elastases AGEs (66.25 ± 0.49%),
which are followed by AGEs of visperlysine (63.27 ± 0.69%). Furthermore, the AgNPs
show high toxicity against HepG2 cell line which shows 53.543% reduction in the
cell viability after 24 h of treatment. The anti-inflammatory activity demonstrated
a potent inhibitory effect of the bio-inspired AgNPs. Overall, the biogenic AgNPs
have the ability to be served for the treatments of anti-aging and also due to
their anti-cancer, antioxidant abilities NPs may be a useful therapy choice for a
variety of disorders including cancer, bacterial infections and other inflammatory
diseases. Moreover, further studies are required in the future to evaluate their in
vivo biomedical applications. Highlights: Biogenic synthesis of AgNPs using
Paraclostridium benzoelyticum Strain for the first time. FTIR analysis confirmed
capping of potent biomolecules which are of great use in applied field especially
Nanomedicines. Notable antimicrobial activity against sinusitis bacteria and
cytotoxic potential of synthesized AgNPs on in vitro basis produce a new idea
shifting us to treat cancerous cell lines. © 2023 Wiley Periodicals LLC.
AN - rayyan-553780430
AU - Faisal, S.
AU - Ullah, R.
AU - Alotaibi, A.
AU - Zafar, S.
AU - Rizwan, M.
AU - Tariq, M. H.
DO - 10.1002/jemt.24362
IS - 7
KW - AgNPs
anti-aging
anti-cancer
anti-inflammatory
Paraclostridium benzoelyticum
Antioxidants
Bacteria
Glycation End Products, Advanced
Metal Nanoparticles
Plant Extracts
Silver
Cell culture
Diseases
Metal nanoparticles
Silver compounds
Ultraviolet spectroscopy
X ray diffraction
advanced glycation end product
antiinfective agent
antioxidant
metal nanoparticle
plant extract
silver
'current
AgNP
Anti-aging
Anti-cancer
Anti-inflammatories
Antibacterials
Biogenics
In-vitro
bacterium
chemistry
PY - 2023
SP - 846-861
ST - Biofabrication of silver nanoparticles employing biomolecules of
Paraclostridium benzoelyticum strain: Its characterization and their in-vitro
antibacterial, anti-aging, anti-cancer and other biomedical applications
T2 - Microscopy Research and Technique
TI - Biofabrication of silver nanoparticles employing biomolecules of
Paraclostridium benzoelyticum strain: Its characterization and their in-vitro
antibacterial, anti-aging, anti-cancer and other biomedical applications
85160814913&doi=10.1002%2fjemt.24362&partnerID=40&md5=029b9468e7d6f4064cf4a40a9d5f3
3fa
VL - 86
ID - 8459
ER -
TY - JOUR
AB - The metabolic syndrome comprises a family of clinical and laboratory
findings, including insulin resistance, hyperglycemia, hypertriglyceridemia, low
high-density lipoprotein cholesterol levels, and hypertension, in addition to
central obesity. The syndrome confers a high risk of cardiovascular mortality.
Indeed, metabolic dysfunction has been shown to cause a direct insult to smooth
muscle and endothelial components of the vasculature, which leads to vascular
dysfunction and hyperreactivity. This, in turn, causes cerebral vasoconstriction
and hypoperfusion, eventually contributing to cognitive deficits. Moreover, the
metabolic syndrome disrupts key homeostatic processes in the brain, including
apoptosis, autophagy, and neurogenesis. Impairment of such processes in the context
of metabolic dysfunction has been implicated in the pathogenesis of
neurodegenerative diseases, including Alzheimer, Parkinson, and Huntington
diseases. The aim of this review is to elucidate the role that the metabolic
syndrome plays in the pathogenesis of the latter disorders, with a focus on the
role of perivascular adipose inflammation in the peripheral-to-central transduction
of the inflammatory insult. This review delineates common signaling pathways that
contribute to these pathologies. Moreover, the role of therapeutic agents aimed at
treating the metabolic syndrome, as well as their risk factors that interfere with
the aforementioned pathways, are discussed as potential interventions for
neurodegenerative diseases.
AN - rayyan-553782311
AU - Fakih, W.
AU - Zeitoun, R.
AU - AlZaim, I.
AU - Eid, A. H.
AU - Kobeissy, F.
AU - Abd-Elrahman, K. S.
AU - El-Yazbi, A. F.
DO - 10.1002/oby.23400
IS - 5
KW - Adipose Tissue/metabolism
Humans
*Insulin Resistance
*Metabolic Syndrome
*Neurodegenerative Diseases/drug therapy
Obesity/complications/drug therapy/metabolism
Neurodegenerative Diseases
LA - eng
N1 - Faculty of Pharmacy, Federation of Translational Medicine of Strasbourg,
University of Strasbourg, Illkirch, France.; Department of Pharmacology and
Toxicology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.;
Department of Pharmacology and Toxicology, Faculty of Medicine, American University
of Beirut, Beirut, Lebanon.; Department of Pharmacology and Toxicology, Faculty of
Medicine, American University of Beirut, Beirut, Lebanon.; Department of
Biochemistry and Molecular Genetics, Faculty of Medicine, American University of
Beirut, Beirut, Lebanon.; Department of Basic Medical Sciences, College of
Medicine, Qatar University, Doha, Qatar.; Biomedical and Pharmaceutical Research
Unit, QU Health, Qatar University, Doha, Qatar.; Department of Biochemistry and
Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut,
Lebanon.; Program for Neurotrauma, Neuroproteomics & Biomarkers Research,
Departments of Emergency Medicine, Psychiatry, Neuroscience and Chemistry,
University of Florida, Gainesville, Florida, USA.; Brain and Mind Research
Institute, Department of Cellular and Molecular Medicine, University of Ottawa,
Ottawa, Ontario, Canada.; Department of Pharmacology and Toxicology, Faculty of
Pharmacy, Alexandria University, Alexandria, Egypt.; Department of Pharmacology and
Therapeutics, College of Medicine and Health Science, Khalifa University, Abu
Dhabi, United Arab Emirates.; Department of Pharmacology and Toxicology, Faculty of
Medicine, American University of Beirut, Beirut, Lebanon.; Department of
Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University,
Alexandria, Egypt.; Department of Pharmacology and Toxicology, Faculty of Pharmacy,
Alamein International University, New Alamein City, Egypt.
PY - 2022
SP - 982-993
ST - Early metabolic impairment as a contributor to neurodegenerative disease:
Mechanisms and potential pharmacological intervention
TI - Early metabolic impairment as a contributor to neurodegenerative disease:
Mechanisms and potential pharmacological intervention
VL - 30
Y2 - 5
ID - 10219
ER -
TY - JOUR
AB - Medicinal plants play a role in the traditional form of providing relief to
several diseases. Couroupita guianensis is also a type of medicinal plant which has
antibacterial, antimycobacterial, antimicrobial, antioxidant, antitumor, antiulcer,
antinociceptive, anthelmintic, antifertility, and antifungal activities. The
chemical constituent of C. guianensis such as indirubin serves as an antibacterial
and antifungal agent because it particularly cures fungal diseases. It is active
for the treatment of chronic myelocytic leukemia. The extract of isatin from the
flower of C. guianensis is also a chemical component that has been used as
prophylactic agent, prevents free radial-induced cancer, acts as a chemotherapeutic
agent to kill cancer cells, and it has antioxidant (act as major defense) and
anticancer activities against human promylocytic leukemia 60 cells. The extract of
chloroform of fruit of C. guianensis shows good antimicrobial activity but low
antimycobacterial activity. The plant extract of C. guianensis is equipotent to
standard drugs such as paracetamol in its analgesic activity and indomethacin in
its anti-inflammatory activity. © 2017 The Authors.
AN - rayyan-553780431
AU - Famina Jasmine, S.
AU - Vinayaga Moorthi, P.
DO - 10.22159/ajpcr.2017.v10i3.16174
IS - 3
KW - Anti biofil
Anti tumor activity
Antibacterial activity
Antioxidant activity
Couroupita quianensis
anthelmintic agent
antifungal agent
antiinfective agent
antimycobacterial agent
antinociceptive agent
antioxidant
antiulcer agent
chloroform
contraceptive agent
Couroupita guianensis extract
fibroblast growth factor receptor 1
methanol
paracetamol
piperazine citrate
plant extract
silver nanoparticle
unclassified drug
Aedes aegypti
anthelmintic activity
antibiofilm ctivity
antifungal activity
antinociception
antiulcer activity
autophosphorylation
Bacillus subtilis
bacterium isolate
Candida albicans
CC50
contraception
Couroupita guianensis
cytotoxicity
drug activity
EC50
enzyme activity
enzyme inhibition
Ericales
Escherichia coli
human
leukocyte migration
Malassezia pachydermatis
Mycobacterium tuberculosis
nonhuman
Plesiomonas shigelloides
Proteus vulgaris
Review
Salmonella enterica serovar Typhi
Vibrio mimicus
wound healing
Humanities
Humanism
Humans
PY - 2017
SP - 50-52
ST - Couroupita guianensis: The reservoir of medicinal compounds of human welfare
T2 - Asian Journal of Pharmaceutical and Clinical Research
TI - Couroupita guianensis: The reservoir of medicinal compounds of human welfare
85014766901&doi=10.22159%2fajpcr.2017.v10i3.16174&partnerID=40&md5=5fdfc5053862f9e4
75cdf9adf409a328
VL - 10
ID - 8460
ER -
TY - JOUR
AB - Alzheimer's disease is a neurodegenerative disorder mainly characterized by
β-amyloid deposit and tau hyperphosphorylation with no curative treatments.
Curcumin (Cur) has been proved to have potential use in Alzheimer's disease with
its anti-amyloid, anti-inflammatory, and anti-oxidant properties, etc. However, its
hydrophobicity and low bioavailability hinder its application. In this paper, we
designed a novel brain-target nanoparticle, poly(lactide-co-glycolide)-block-
poly(ethylene glycol) (PLGA-PEG) conjugated with B6 peptide and was loaded with Cur
(PLGA-PEG-B6/Cur) and administered it into HT22 cells and APP/PS1 Al transgenic
mice. The in vitro assays including dynamic light scattering (DLS), flow cytometry
(FCM), red blood cell (RBC) lysis, and thromboelastography (TEG) analysis indicated
that this nanoparticle could narrow the diameter of Cur, increase its cellular
uptake and possess good blood compatibility. The results from Morris water maze
proved that PLGA-PEG-B6/Cur could tremendously improve the spatial learning and
memory capability of APP/PS1 mice, compared with native Cur. The ex vivo assays
including Bielschowsky silver staining, immunostaining, and western blotting
demonstrated that PLGA-PEG-B6/Cur could reduce hippocampal β-amyloid formation and
deposit and tau hyperphosphorylation. Thus, we suggested that PLGA-PEG-B6/Cur
nanoparticles would be of potential and promising use for the treatment of
Alzheimer's disease.
AN - rayyan-553781758
AU - Fan, S.
AU - Zheng, Y.
AU - Liu, X.
AU - Fang, W.
AU - Chen, X.
AU - Liao, W.
AU - Jing, X.
AU - Lei, M.
AU - Tao, E.
AU - Ma, Q.
AU - Zhang, X.
AU - Guo, R.
AU - Liu, J.
DO - 10.1080/10717544.2018.1461955
IS - 1
J2 - Drug Deliv
KW - Alzheimer Disease/*drug therapy
Animals
Biological Availability
Cell Line
Curcumin/*chemistry/pharmacology
Drug Carriers/chemistry
Drug Compounding
Humans
Hydrophobic and Hydrophilic Interactions
Mice
Molecular Targeted Therapy
Nanoparticles/*chemistry
Oligopeptides/chemistry/metabolism/*pharmacology
Polyesters/*chemistry
Polyethylene Glycols/*chemistry
Alzheimer Disease
C-Peptide
LA - eng
N1 - a Department of Neurology , Sun Yat-sen Memorial Hospital, Sun Yat-sen
University , Guangzhou , China.; a Department of Neurology , Sun Yat-sen Memorial
Hospital, Sun Yat-sen University , Guangzhou , China.; b Key Laboratory of
Biomaterials of Guangdong Higher Education Institutes Department of Biomedical
Engineering , Jinan University , Guangzhou , China.; a Department of Neurology ,
Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou , China.; a
Department of Neurology , Sun Yat-sen Memorial Hospital, Sun Yat-sen University ,
Guangzhou , China.; c Zhongshan City People's Hospital , Zhongshan City , Guangdong
Province , China.; a Department of Neurology , Sun Yat-sen Memorial Hospital, Sun
Yat-sen University , Guangzhou , China.; a Department of Neurology , Sun Yat-sen
Memorial Hospital, Sun Yat-sen University , Guangzhou , China.; a Department of
Neurology , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou ,
China.; a Department of Neurology , Sun Yat-sen Memorial Hospital, Sun Yat-sen
University , Guangzhou , China.; d Department of Neurology , University of
California , Los Angeles , CA , USA.; e Applied Immunology and Immunotherapy,
Department of Clinical Neuroscience , Karolinska Institute, Center for Molecular
Medicine, Karolinska University Hospital at Solna , Stockholm , Sweden.; b Key
Laboratory of Biomaterials of Guangdong Higher Education Institutes Department of
Biomedical Engineering , Jinan University , Guangzhou , China.; a Department of
Neurology , Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou ,
China.; f Laboratory of RNA and Major Diseases of Brain and Heart , Sun Yat-sen
Memorial Hospital, Sun Yat-sen University , Guangzhou , China.; g Guangdong
Province Key Laboratory of Brain Function and Disease , Zhongshan School of
Medicine, Sun Yat-sen University , Guangzhou , China.
PY - 2018
SP - 1091-1102
ST - Curcumin-loaded PLGA-PEG nanoparticles conjugated with B6 peptide for
potential use in Alzheimer's disease
T2 - Drug delivery
TI - Curcumin-loaded PLGA-PEG nanoparticles conjugated with B6 peptide for
potential use in Alzheimer's disease
VL - 25
Y2 - 11
ID - 9691
ER -
TY - JOUR
AB - Low efficiency of targeting and delivery toward the thrombus site poses
challenges to using thrombolytic drugs. Inspired by the biomimetic system of
platelet membranes (PMs) and glucose oxidase (GOx) modification technologies, we
develop a novel GOx-powered Janus nanomotor by asymmetrically attaching the GOx to
polymeric nanomotors coated with the PMs. Then the PM-coated nanomotors were
conjugated with urokinase plasminogen activators (uPAs) on their surfaces. The PM-
camouflaged design conferred excellent biocompatibility to the nanomotors and
improved their targeting ability to thrombus. The Janus distribution of GOx also
allows the uneven decomposition of glucose in biofluids to produce a chemophoretic
motion, increasing the drug delivery efficiency of nanomotors. In addition, these
nanomotors are located at the lesion site due to the mutual adhesion and
aggregation of platelet membranes. Furthermore, thrombolysis effects of nanomotors
are enhanced in static and dynamic thrombus as well as in mouse models. It is
believed that the novel PM-coated enzyme-powered nanomotors represent a great value
for thrombolysis treatment. © 2023 American Chemical Society.
AN - rayyan-553780436
AU - Fang, X.
AU - Ye, H.
AU - Shi, K.
AU - Wang, K.
AU - Huang, Y.
AU - Zhang, X.
AU - Pan, J.
DO - 10.1021/acsbiomaterials.3c00387
IS - 7
KW - GOx
Janus
nanomotor
platelet
thrombus therapy
uPAs
Animals
Blood Platelets
Fibrinolytic Agents
Glucose Oxidase
Mice
Polymers
Thrombosis
Biocompatibility
Biomimetics
Blood vessels
Disease control
Efficiency
Glucose
Glucose sensors
Nanotechnology
Platelets
adenosine diphosphate
carrageenan
CD47 antigen
fibrinogen receptor
fibrinolytic agent
glucose oxidase
nanoparticle
PADGEM protein
sodium chloride
thrombin
urokinase
polymer
Nanomotors
Plasminogen activators
Platelet membranes
Targeted delivery
Thrombolysis
Thrombolytic
Thrombus therapy
Urokinase plasminogen
Urokinase plasminogen activator
animal cell
Article
biocompatibility
biosafety
blood clot lysis
blood toxicity
cell viability
coating (procedure)
conjugation
controlled study
cytotoxicity
decomposition
female
gel electrophoresis
HUVEC cell line
in vitro study
in vivo study
inflammatory cell
leukocyte count
lymphocyte count
monocyte
mouse
MTT assay
neutrophil count
nonhuman
protein content
protein fingerprinting
silver staining
surface property
thrombocyte membrane
thrombosis
TUNEL assay
Western blotting
animal
thrombocyte
Glucose oxidase
PY - 2023
SP - 4302-4310
ST - GOx-Powered Janus Platelet Nanomotors for Targeted Delivery of Thrombolytic
Drugs in Treating Thrombotic Diseases
T2 - ACS Biomaterials Science and Engineering
TI - GOx-Powered Janus Platelet Nanomotors for Targeted Delivery of Thrombolytic
Drugs in Treating Thrombotic Diseases
85163565601&doi=10.1021%2facsbiomaterials.3c00387&partnerID=40&md5=810554614467a448
6d276ff18099bc92
VL - 9
ID - 8465
ER -
TY - JOUR
AB - American Cutaneous Leishmaniasis (ACL) is a zoonosis caused by Leishmania
protozoa. The ACL chemotherapy available is unsatisfactory motivating researches to
seek alternative treatments. In this study, we investigated the action of biogenic
silver nanoparticle (AgNp-bio) obtained from Fusarium oxysporium, against
Leishmania amazonensis promastigote and amastigote forms. The AgNp-bio promastigote
treatment results in promastigote death leading to apoptosis-like events due an
increased production of reactive oxygen species (ROS), loss of mitochondrial
integrity, phosphatidylserine exposure and damage on promastigotes membrane. In L.
amazonensis infected macrophages, AgNp-bio treatment was still able to reduce the
percentage of infected macrophages and the amount of amastigotes per macrophage,
consequently, the amount of promastigotes recovered. This leishmanicidal effect was
also accompanied by a decrease in the levels of ROS and nitric oxide. By observing
the ultrastructural integrity of the intracellular amastigotes, we found that the
AgNp-bio treatment made a significant damage, suggesting that the compound has a
direct effect on intracellular amastigotes. These results demonstrated that AgNp-
bio had a direct effect against L. amazonensis forms and acted on immunomodulatory
ability of infected macrophages, reducing the infection without inducing the
synthesis of inflammatory mediators, which continuous stimulation can generate and
aggravate leishmaniotic lesions. Overall, our findings suggest that the use of
AgNp-bio stands out as a new therapeutic option to be considered for further in
vivo investigations representing a possible treatment for ACL. © 2017 Elsevier B.V.
AN - rayyan-553780437
AU - Fanti, J. R.
AU - Tomiotto-Pellissier, F.
AU - Miranda-Sapla, M. M.
AU - Cataneo, A. H. D.
AU - Andrade, C. G. T. D. J.
AU - Panis, C.
AU - Rodrigues, J. H. D. S.
AU - Wowk, P. F.
AU - Kuczera, D.
AU - Costa, I. N.
AU - Nakamura, C. V.
AU - Nakazato, G.
AU - Durán, N.
AU - Pavanelli, W. R.
AU - Conchon-Costa, I.
DO - 10.1016/j.actatropica.2017.10.027
KW - Immunomodulation
Leishmaniasis
Nanoparticles
Nanotechnology
Reactive oxygen species (ROS)
Animals
Antiprotozoal Agents
Apoptosis
Leishmania
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Silver
Fusarium oxysporum
Leishmania amazonensis
Protozoa
nitric oxide
nitrite
phosphatidylserine
silver nanoparticle
antiprotozoal agent
metal nanoparticle
silver
apoptosis
cell organelle
immune system
nanoparticle
parasite
protist
reactive oxygen species
amastigote
animal cell
Article
cell damage
cell death
controlled study
death
dose response
drug activity
exposure
in vitro study
leishmanicidal effect
macrophage
membrane damage
mouse
necrosis
nonhuman
promastigote
zeta potential
animal
Bagg albino mouse
drug effect
leishmaniasis
PY - 2018
SP - 46-54
ST - Biogenic silver nanoparticles inducing Leishmania amazonensis promastigote
and amastigote death in vitro
T2 - Acta Tropica
TI - Biogenic silver nanoparticles inducing Leishmania amazonensis promastigote
and amastigote death in vitro
85032457954&doi=10.1016%2fj.actatropica.2017.10.027&partnerID=40&md5=e09f0013b1fca2
2c2ba11bb7e44b89b3
VL - 178
ID - 8466
ER -
TY - JOUR
AB - Liver ischemia-reperfusion injury (IRI) is a pathophysiological insult that
often occurs during liver surgery. Blackberry leaves are known for their anti-
inflammatory and antioxidant activities. Aims: To achieve site-specific delivery of
blackberry leaves extract (BBE) loaded AgNPs to the hepatocyte in IRI and to verify
possible molecular mechanisms. Methods: IRI was induced in male Wister rats. Liver
injury, hepatic histology, oxidative stress markers, hepatic expression of
apoptosis-related proteins were evaluated. Non-targeted metabolomics for chemical
characterization of blackberry leaves extract was performed. Key findings: Pre-
treatment with BBE protected against the deterioration caused by I/R, depicted by a
significant improvement of liver functions and structure, as well as reduction of
oxidative stress with a concomitant increase in antioxidants. Additionally, BBE
promoted phosphorylation of antiapoptotic proteins; PI3K, Akt and mTOR, while
apoptotic proteins; Bax, Casp-9 and cleaved Casp-3 expressions were decreased. LC-
HRMS-based metabolomics identified a range of metabolites, mainly flavonoids and
anthocyanins. Upon comprehensive virtual screening and molecular dynamics
simulation, the major annotated anthocyanins, cyanidin and pelargonidin glucosides,
were suggested to act as PLA2 inhibitors. Significance: BBE can ameliorate hepatic
IRI augmented by BBE-AgNPs nano-formulation via suppressing, oxidative stress and
apoptosis as well as stimulation of PI3K/Akt/mTOR signaling pathway. © 2023 by the
authors.
AN - rayyan-553780440
AU - Fathi, A. M.
AU - Waz, S.
AU - Alaaeldin, E.
AU - Toni, N. D. M.
AU - El-Sheikh, A. A. K.
AU - Sayed, A. M.
AU - Abdelmohsen, U. R.
AU - Nazmy, M. H.
DO - 10.3390/metabo13030419
IS - 3
KW - AgNPs
blackberry
caspase-3
cell apoptosis
liver ischemia-reperfusion injury
metabolomics
PI3K/Akt/mTOR
anthocyanin
blackberry extract
caspase 3
caspase 9
catalase
cyanidin chloride
flavonoid
glucoside
glutathione
malonaldehyde
plant extract
protein Bax
protein bcl 2
quercetin
silver nanoparticle
silymarin
unclassified drug
urethan
Akt signaling
animal experiment
animal model
animal tissue
apoptosis
Article
cell survival
controlled study
cryopreservation
hepatic ischemia reperfusion injury
histopathology
hypoxia
immunoblotting
immunoreactivity
laparotomy
lipid peroxidation
liver function
liver histology
liver toxicity
male
molecular dynamics
MTT assay
nonhuman
oxidative stress
Pi3K/Akt signaling
protein expression
rat
TUNEL assay
upregulation
Western blotting
zeta potential
PY - 2023
ST - Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via
PI3K/Akt/mTOR Pathway
T2 - Metabolites
TI - Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via
PI3K/Akt/mTOR Pathway
85151495875&doi=10.3390%2fmetabo13030419&partnerID=40&md5=d72994ba47651b732d21393c3
a06bfeb
VL - 13
ID - 8469
ER -
TY - JOUR
AB - Hydraulic fracturing (“fracking”) is used in unconventional gas drilling to
allow for the free flow of natural gas from rock. Sand in fracking fluid is pumped
into the well bore under high pressure to enter and stabilize fissures in the rock.
In the process of manipulating the sand on site, respirable dust (fracking sand
dust, FSD) is generated. Inhalation of FSD is a potential hazard to workers
inasmuch as respirable crystalline silica causes silicosis, and levels of FSD at
drilling work sites have exceeded occupational exposure limits set by OSHA. In the
absence of any information about its potential toxicity, a comprehensive rat animal
model was designed to investigate the bioactivities of several FSDs in comparison
to MIN-U-SIL® 5, a respirable α-quartz reference dust used in previous animal
models of silicosis, in several organ systems (Fedan, J.S., Toxicol Appl Pharmacol.
00, 000–000, 2020). The present report, part of the larger investigation,
describes: 1) a comparison of the physico-chemical properties of nine FSDs,
collected at drilling sites, and MIN-U-SIL® 5, a reference silica dust, and 2) a
comparison of the pulmonary inflammatory responses to intratracheal instillation of
the nine FSDs and MIN-U-SIL® 5. Our findings indicate that, in many respects, the
physico-chemical characteristics, and the biological effects of the FSDs and MIN-U-
SIL® 5 after intratracheal instillation, have distinct differences. © 2020 Elsevier
Inc.
AN - rayyan-553780442
AU - Fedan, J. S.
AU - Hubbs, A. F.
AU - Barger, M.
AU - Schwegler-Berry, D.
AU - Friend, S. A.
AU - Leonard, S. S.
AU - Thompson, J. A.
AU - Jackson, M. C.
AU - Snawder, J. E.
AU - Dozier, A. K.
AU - Coyle, J.
AU - Kashon, M. L.
AU - Park, J. H.
AU - McKinney, W.
AU - Roberts, J. R.
DO - 10.1016/j.taap.2020.115282
KW - Fracking sand dust
MIN-U-SIL®
Particle characterization
Rat model
Silica
Air Pollutants, Occupational
Animals
Dust
Hydraulic Fracking
Inhalation Exposure
Lung
Male
Occupational Exposure
Pneumonia
Quartz
Rats
Sand
Silicon Dioxide
Silicosis
Trachea
aluminum
antimony
arsenic
barium
beryllium
cadmium
chromium
cobalt
copper
endotoxin
iron
lanthanum
lead
lithium
magnesium
manganese
phosphorus
silicon dioxide
silver
strontium
tellurium
thallium
tin
titanium
unindexed drug
vanadium
yttrium
zinc
zirconium
animal cell
animal experiment
animal model
animal tissue
Article
biological activity
cell hyperplasia
comparative study
controlled study
crystallization
dust
electron spin resonance
elemental analysis
enzyme activity
eosinophil
fracking
histiocytosis
histopathology
hypertrophy
inflammation
inhalation
lung alveolus epithelium cell
lung alveolus macrophage
lung fibrosis
lung lavage
lung parenchyma
lung toxicity
lymphocyte
male
neutrophil
nonhuman
particle size
physical chemistry
rat
respirable particulate matter
sand
silicosis
X ray diffraction
adverse event
air pollutant
animal
chemistry
disease model
drug effect
exposure
lung
pneumonia
procedures
Sprague Dawley rat
trachea
PY - 2020
ST - Biological effects of inhaled hydraulic fracturing sand dust. II. Particle
characterization and pulmonary effects 30 d following intratracheal instillation
T2 - Toxicology and Applied Pharmacology
TI - Biological effects of inhaled hydraulic fracturing sand dust. II. Particle
characterization and pulmonary effects 30 d following intratracheal instillation
85094567790&doi=10.1016%2fj.taap.2020.115282&partnerID=40&md5=b98965d8e36ec318b8b9b
bd37ce02853
VL - 409
ID - 8471
ER -
TY - JOUR
AB - Silica (SiO2) nanoparticles (NPs) have found extensive applications in
industrial manufacturing, biomedical and biotechnological fields. Therefore, the
increasing exposure to such ultrafine particles requires studies to characterize
their potential cytotoxic effects in order to provide exhaustive information to
assess the impact of nanomaterials on human health. The understanding of the
biological processes involved in the development and maintenance of a variety of
pathologies is improved by genome-wide approaches, and in this context, gene set
analysis has emerged as a fundamental tool for the interpretation of the results.
In this work we show how the use of a combination of gene-by-gene and gene set
analyses can enhance the interpretation of results of in vitro treatment of A549
cells with Ludox (R) colloidal amorphous silica nanoparticles. By gene-by-gene and
gene set analyses, we evidenced a specific cell response in relation to NPs size
and elapsed time after treatment, with the smaller NPs (SM30) having higher impact
on inflammatory and apoptosis processes than the bigger ones. Apoptotic process
appeared to be activated by the up-regulation of the initiator genes TNFa and IL1b
and by ATM. Moreover, our analyses evidenced that cell treatment with Ludox (R)
silica nanoparticles activated the matrix metalloproteinase genes MMP1, MMP10 and
MMP9. The information derived from this study can be informative about the
cytotoxicity of Ludox (R) and other similar colloidal amorphous silica NPs prepared
by solution processes.
AN - rayyan-553780443
AU - Fede, C.
AU - Millino, C.
AU - Pacchioni, B.
AU - Celegato, B.
AU - Compagnin, C.
AU - Martini, P.
AU - Selvestrel, F.
AU - Mancin, F.
AU - Celotti, L.
AU - Lanfranchi, G.
AU - Mognato, M.
AU - Cagnin, S.
DO - 10.3390/ijerph110908867
IS - 9
KW - Humanities
Humanism
Humans
PY - 2014
SN - 1660-4601
SP - 8867-8890
ST - Altered Gene Transcription in Human Cells Treated with Ludox (R) Silica
Nanoparticles
T2 - INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
TI - Altered Gene Transcription in Human Cells Treated with Ludox (R) Silica
Nanoparticles
VL - 11
Y2 - 9
ID - 8472
ER -
TY - JOUR
AN - rayyan-553782245
AU - Felice, C.
AU - Lewis, A.
AU - Iqbal, S.
AU - Gordon, H.
AU - Rigoni, A.
AU - Colombo, M. P.
AU - Armuzzi, A.
AU - Feakins, R.
AU - Lindsay, J. O.
AU - Silver, A.
DO - 10.1016/j.jcmgh.2020.11.009
IS - 3
J2 - Cell Mol Gastroenterol Hepatol
KW - Acetylation/drug effects
Adolescent
Adult
Aged
Animals
Biopsy
Cohort Studies
Colitis, Ulcerative/chemically induced/*drug therapy/genetics/pathology
Colon/drug effects/immunology/pathology
Crohn Disease/*drug therapy/genetics/pathology
Dextran Sulfate/administration & dosage/toxicity
Epigenesis, Genetic/drug effects
Female
Histone Deacetylase Inhibitors/pharmacology/*therapeutic use
Histones/*metabolism
Humans
Intestinal Mucosa/drug effects/immunology/pathology
Male
Mice
Middle Aged
Valproic Acid/pharmacology/*therapeutic use
Young Adult
Lysine
Intestines
Histones
Inflammation
LA - eng
N1 - Centre for Genomics and Child Health, Blizard Institute, Barts and The London
School of Medicine and Dentistry, QMUL, London, United Kingdom; Department of
Internal Medicine, University of Padua, Internal Medicine 1 Unit, Ca' Foncello
Hospital, Treviso, Italy.; Centre for Genomics and Child Health, Blizard Institute,
Barts and The London School of Medicine and Dentistry, QMUL, London, United
Kingdom.; Centre for Genomics and Child Health, Blizard Institute, Barts and The
London School of Medicine and Dentistry, QMUL, London, United Kingdom.; Centre for
Immunobiology, Blizard Institute, Barts and The London School of Medicine and
Dentistry, QMUL, London, United Kingdom.; Molecular Immunology Unit, Department of
Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale
dei Tumori, Milan, Italy.; Molecular Immunology Unit, Department of Experimental
Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori,
Milan, Italy.; IBD Unit, Gemelli Hospital Foundation-Catholic University, Rome,
Italy.; Department of Cellular Pathology, Royal Free London NHS Foundation Trust,
London, United Kingdom.; Centre for Immunobiology, Blizard Institute, Barts and The
London School of Medicine and Dentistry, QMUL, London, United Kingdom.; Centre for
Genomics and Child Health, Blizard Institute, Barts and The London School of
Medicine and Dentistry, QMUL, London, United Kingdom. Electronic address:
a.r.silver@qmul.ac.uk.
PY - 2021
SP - 889-891.e6
ST - Intestinal Inflammation is Linked to Hypoacetylation of Histone 3 Lysine 27
and can be Reversed by Valproic Acid Treatment in Inflammatory Bowel Disease
Patients
T2 - Cellular and molecular gastroenterology and hepatology
TI - Intestinal Inflammation is Linked to Hypoacetylation of Histone 3 Lysine 27
and can be Reversed by Valproic Acid Treatment in Inflammatory Bowel Disease
Patients
VL - 11
ID - 10155
ER -
TY - GEN
AB - O agregado de trióxido mineral (MTA) tem sido amplamente utilizado em
Endodontia, devido à sua boa adaptação marginal e biocompatibilidade. Na tentativa
de associar propriedades físico-químicas de um cimento obturador de canal radicular
a biocompatibilidade do MTA, algumas modificações foram feitas no MTA para
possibilitar seu uso como um cimento obturador de canal radicular. Assim, a reação
de promovida por um cimento a base de MTA (MTA Fillapex) em subcutâneo de ratos foi
investigada por análise morfológica e morfométrica. Oitenta ratos foram
distribuídos em 4 grupos (n=20); em cada animal, um tubos de polietileno preenchido
com MTA Fillapex, MTA, AH Plus ou Fill Canal foi implantado no subcutânea dorsal.
Após 7, 15, 30 e 60 dias, os tubos rodeados por tecido conjuntivo foram removidos,
fixados em formaldeído 4% e incluídos em parafina. Nos cortes corados com HE, foi
avaliada a densidade numérica de células inflamatórias na cápsula e análises
estatísticas realizadas usando ANOVA e do Student-Newman-Keuls (p≤0.05). Alguns
cortes foram submetidos a imuno-histoquímica para marcação de macrófagos; o método
de von Kossa foi usado para detecção de estruturas calcificadas. Nossos resultados
revelaram que, nos períodos de 7 para 15 dias, o número de células inflamatórias
foi significantemente mais elevado na cápsula do MTA Fillapex em comparação com
outros materiais. No entanto, uma redução significante na densidade numérica de
células inflamatórias foi verificada na cápsula do MTA Fillapex aos 30 e 60 dias
quando comparado com os períodos iniciais. Aos 30 dias, macrófagos imunopositivos
foram observados principalmente na superfície da cápsula em íntima justaposição ao
MTA Fillapex, MTA e AH Plus. No entanto, o intenso processo inflamatório crônico
adjacente ao Fill Canal exibiu numerosas células imunopositivas por toda extensão
da cápsula. No período de 60 dias, a reação inflamatória promovida pelo MTA
Fillapex foi semelhante do MTA e significantemente menor ao Fill Canal de
preenchimento. Em contrapartida, observou-se um aumento gradual e significante de
células inflamatórias na cápsula adjacente ao Fill Canal. Estruturas von Kossa-
positivas foram observadas na cápsula adjacente para o MTA Fillapex, o MTA e AH
Plus; estruturas positivas não foram encontradas na cápsula ao redor do Fill Canal.
Os nossos resultados indicam que MTA Fillapex apresente, no período de 60 dias,
biocompatibilidade semelhante ao MTA Mineral Trioxide Aggregate (MTA) has been
widely used in Endodontics due to its good marginal adaptation and
biocompatibility. In attempt to associate physicochemical properties of a root
canal sealer with biocompatibility of the MTA, some modifications have been made in
the MTA to provide its use as a root canal sealer. Thus, the tissue reaction
promoted by a MTA-based sealer (MTA-Fillapex) in rat subcutaneous was investigated
by morphological and morphometric analyses. Eighty male rats were distributed into
4 groups (n=20); in each animal, one polyethylene tubes filled with MTA-Fillapex,
MTA, AH-Plus or Fill Canal was implanted in the dorsal subcutaneous. After 7, 15,
30 and 60 days, the tubes surrounded by connective tissue were removed, fixed in 4%
formaldehyde and embedded in paraffin. In the HE-stained sections, the numerical
density of inflammatory cells in the capsule was evaluated and statistical analyses
performed using ANOVA and Student-Newman-Keuls test (p≤0.05). Sections were
submitted to immunohistochemistry for the detection of macrophage; von Kossa method
was used for detection of calcified structures. Our results revealed that, in the
periods of 7 and 15 days, the number of inflammatory cells was significantly higher
in the capsule of the MTA-Fillapex in comparison to other materials. However,
significant reduction in the density numerical of inflammatory cells was verified
in the capsule of the MTA-Fillapex in the 30 and 60 days when compared to the
initial periods. At 30 days, immunolabelling for macrophages was mainly det cted in
the surface of capsule in close juxtaposition to the MTA Fillapex, MTA and AH Plus.
However, the intense chronic inflammatory process adjacent to the Fill Canal
exhibited numerous immunolabelled cells. At 60 days, the inflammatory reaction
promoted by MTA Fillapex was similar to the MTA and significantly lower than Fill
Canal. Otherwise, a gradual and significant increase was observed in the number of
inflammatory cells in the capsule adjacent to the Fill Canal. Structures von
Kossapositive were observed in the capsule adjacent to the MTA-Fillapex, MTA and
AHPlus; positive structures were not seen in the capsule surrounding the Fill
Canal. In the period of 60 days, our results indicate that MTA-Fillapex, exhibits
biocompatibility similar to MTA
AN - rayyan-553780450
AU - Ferino, Rafael Vicente
KW - Endodontia
Endodontics
Imunoistoquímica
Material testing
Ratos
Rats
Teste de materiais
LA - pt
PY - 2012
SP - 60-60
ST - Reação histológica ao cimento endodôntico à base de MTA (Fillapex®) em
subcutâneo de ratos
TI - Reação histológica ao cimento endodôntico à base de MTA (Fillapex®) em
subcutâneo de ratos
UR -
https://repositorio.unesp.br/bitstream/handle/11449/90405/ferino_rv_me_arafo.pdf?
sequence=1&isAllowed=y
ID - 8479
ER -
TY - GEN
AB - A raiva é uma doença do sistema nervoso central que é quase invariavelmente
fatal. Apesar de causar cerca de 60.000 mortes/ano, a raiva ainda permanece uma
doença negligenciada na maioria dos países, principalmente naqueles em
desenvolvimento. O objetivo do nosso estudo foi verificar nos microambientes
meningeal, perivascular e intraparenquimatoso do sistema nervoso central, o
processo inflamatório, a resposta imune do hospedeiro e a morte dos neurônios
frente à infecção rábica transmitida por morcegos. Verificamos que a raiva humana
transmitida por morcegos é uma meningoencefalomielite. Através de reação imuno-
histoquímica caracterizamos e quantificamos in situ a distribuição do antígeno
viral, o fenótipo de células inflamatórias, as células expressando citocinas pró
inflamatórias, citocinas representativas de padrão Th1 e Th2 e células em apoptose.
O antígeno viral foi encontrado difusamente no parênquima cerebral, em maior
abundância em neurônios, não diferindo sua distribuição em relação as regiões
cerebrais. As células da glia, em especial os astrócitos, estavam imunomarcadas com
o antígeno da raiva, assim como as células endoteliais e células mononucleadas da
luz vascular. Esses achados contribuíram para a hipótese da ocorrência de uma via
hematogênica alternativa de disseminação viral, através da infecção de células
endoteliais pelo vírus, posterior infecção de astrócitos e finalmente infecção de
neurônios. A expressão significativa de IL-12 nos pacientes rábicos provavelmente
traduz imunidade de base preservada. Identificamos, outrossim, falta de resposta
efetiva das células NK e comprometimento da resposta imune adaptativa sequencial,
demonstrada pela depleção de linfócitos TCD4+ no parênquima cerebral, prejuízo da
atividade citotóxica dos linfócitos TCD8+ com proporcionalmente baixa expressão de
granzima e expressão rarefeita de IFN-g e IL-2r. Essa situação deve ser reflexo da
manipulação do vírus sobre a resposta imune inata e adaptativa... Viral disease of
central nervous system almost invariable fatal, rabies causes about 60.000 deaths
yearly, and still remain a neglected disease in most countries, specially in
developing ones. We study the meningeal, perivascular and parenquimal environment
in central nervous system from patients who dies after bat transmitted rabies,
looking for the inflammatory process, host immune response and neuronal death. We
show that human rabies transmitted by bats is a meningoencephalomyelitis.
Immunohistochemistry allows the in situ quantification of viral antigen
distribution, inflammatory cellular phenotype, expression of cytokines representing
both Th1 and Th2 profile and pro-inflammatory and cell apoptosis. Viral antigen was
found disseminatted in cerebral parenquima, more abundant in neurons, without
cerebral area preference. Glial cells, specially astrocytes, were immunostained
with rabies antigen, as well as endothelial and vascular mononuclear cells. These
findings contributed for an alternative hypothesis of the occurrence of hematogenic
viral dissemination, through viral infection or passage by endothelial cells,
followed by astrocyte infection, and finally reaching neurons. IL-12 significant
expression in central nervous system from rabies patients probably reflect
preservation of immunity. The lack of effective NK cells could compromise adaptive
immune response, demonstred by TCD4+ lymphocytes depletion in cerebral parenquima,
ineffective TCD8+ cytotoxic activity with low granzyme expression and low
expression of IFN-g and IL-2r. This situation reflects viral effect on host innate
and adaptive immune response leading to an ineffective response for viral
clearance. High pro-inflammatory cytokine expression, IL- 1b, IL-6 and TNF-a,
contribute for neuronal damage, with predominant Th2 cytokine profile in central
nervous system in rabies patients, with high expression of IL-4 and IL-10.
Increased TGF-b expression also shows an immune suppressor...
AN - rayyan-553780452
AU - Fernandes, Elaine Raniero
KW - Central nervous system
Chiroptera
Imunoistoquímica
Inflamação
Inflammation
Quirópteros
Rabies
Raiva
Sistema nervoso central
LA - pt
PY - 2009
SP - [275]-[275]
ST - O processo inflamatório, a resposta imune in situ e a morte neuronal em
sistema nervoso central de pacientes com raiva transmitida por morcegos
TI - O processo inflamatório, a resposta imune in situ e a morte neuronal em
sistema nervoso central de pacientes com raiva transmitida por morcegos
UR - http://www.teses.usp.br/teses/disponiveis/5/5144/tde-09122009-171548/
publico/ElaineRanieroFernandesDoutorado.pdf
ID - 8481
ER -
TY - GEN
AB - O objetivo deste estudo foi avaliar a capacidade de produção de
nanopartículas de prata através do extrato da casca de romã, e produzir formulações
contendo estas nanopartículas para uso em feridas. Elas foram testadas quanto à
ação antimicrobiana, citotóxica e potencial cicatrizante. Para a produção das
nanopartículas de prata propôs-se uma síntese utilizando-se como base duas
metodologias já estabelecidas na literatura, utilizando-se para reação
carboximetilcelulose, propilenoglicol, nitrato de prata, água e extrato da casca de
romã como agente redutor. O extrato da casca de romã foi caracterizado em
parâmetros como pH, massa seca e quantidade de taninos bioativos (ácido elágico e
totais fenólicos expressos em ácido gálico). Os totais fenólicos do extrato foram
também dosados após seu aquecimento nas diferentes condições de tempo e temperatura
propostos para as sínteses das nanopartículas de prata (12 minutos, 1 hora e 2
horas, à 50ºC e 100ºC). As nanopartículas de prata produzidas foram, então,
adicionadas a uma solução contendo compostos para produção de formulações para
serem utilizadas no tratamento de feridas. Elas foram caracterizadas através de
espectroscopia UV-Visível, microscopia eletrônica de varredura (MEV), potencial
zeta e dosagem de íons remanescentes após as reações. A atividade antimicrobiana
tanto das nanopartículas como de suas formulações contra Candida albicans SC 5314 e
Staphylococcus aureus ATCC 25923 foi avaliada por meio do método da microdiluição.
Na avaliação dos extratos, apesar de ocorrer um aumento na concentração de totais
fenólicos com o aumento da temperatura, a concentração inibitória mínima (CIM)
manteve-se estável com valores de 391 µg/ml e 781 µg/ml para S. aureus e C.
albicans. A formação das nanopartículas de prata foi confirmada com a formação de
picos característicos na espectroscopia UV-Visível e pelas imagens de MEV, onde
verificou-se que a síntese com tempo de reação de 12 minutos e aquecimento a 50ºC
gerou nanopartículas mais uniformes e melhor distribuídas na formulação. As
sínteses propostas promoveram a redução iônica da prata de aproximadamente 100%,
independente do tempo temperatura utilizados na reação. Valores de CIM para as
nanopartículas de prata foram de 67,50 µg/ml e 68,75 µg/ml respectivamente para S.
aureus e C. albicans independente das variações das condições de síntese. Após a
seleção da síntese das nanopartículas de prata por 12 min à 50ºC, estas partículas
foram também caracterizadas por difração de raios-X e microscopia eletrônica de
transmissão (TEM), e as respectivas formulações por meio de espalhamento de luz
dinâmica, dosagem de íons livres, potencial zeta, MEV e TEM. Para essas formulações
foi também realizado um teste de estabilidade variando-se umidade e temperatura.
Além da atividade antimicrobiana contra Candida albicans SC 5314 e Staphylococcus
aureus ATCC 25923, a citotoxicidade em fibroblastos (L929) das nanopartículas de
prata e das formulações destas partículas e do extrato da casca de romã foram
também avaliadas. Para os extratos foram observados valores de 3,13, 86,39±0,96%
m/m, 3,64±0,03 mg/g, 392,0±9 mg/g respectivamente para pH, massa seca, ácido
elágico e totais fenólicos. Como controle, foram produzidas nanopartículas de prata
sintetizadas convencionalmente (AgNP química), e observou-se potencial redutor de
99,89% e 99,51% para as sínteses utilizando-se extrato de romã (AgNP green) e um
agente redutor químico convencional (AgNP química). Verificou-se a formação de
partículas com tamanhos médios de 89 e 19 nm para nanopartículas green e química. A
formulação contendo as nanopartículas de prata apresentaram um potencial
antimicrobiano expressivamente maior do que o princípio ativo isolado, sendo 255 e
4 vezes mais efetiva contra S. aureus e C. albicans, respectivamente. Os valores de
citotoxicidade foram consideravelmente menores para as nanopartículas de prata
sintetizadas pelo extr to de romã quando comparadas as produzidas
convencionalmente. De acordo com os valores de CIM e da citotoxicidade, a
concentração das formulações foi ajustada, gerando assim três formulações: i) AgNP
green, ii) AgNP química e iii) extrato de romã, nas concentrações de 337,5 µg/ml,
5,55 µg/ml e 94 µg/ml respectivamente. Para o estudo in vivo, utilizou-se como
controle um medicamento comercial contendo prata indicado para tratamento de
feridas (Sulfadiazina de prata). Foram selecionados noventa ratos Wistar machos com
peso médio de 180 gramas. Foi induzida diabetes nos ratos, e, em seguida, realizou-
se duas incisões no dorso dos animais e as lesões foram imediatamente infectadas
com S. aureus (ATCC 25923) e C. albicans (SC 5314). Após 24 h, os animais foram
divididos em grupos de acordo com as formulações propostas em cada tratamento,
seguindo-se um protocolo de duas vezes ao dia por 2, 7 e 14 dias. Após o período de
tratamento os animais foram eutanasiados e verificado o potencial reparador através
do índice de fechamento de ferida, avaliação do infiltrado inflamatório,
angiogenese, mieloperoxidase e hidroxiprolina. Ainda foi verificado o potencial
antimicrobiano das formulações através da contagem de células viáveis de cada
microrganismo infectado nas feridas. De forma geral, as formulações contendo
nanopartículas de prata mostraram os melhores resultados para o fechamento das
feridas, apresentando ainda uma atividade anti-inflamatória maior que a do extrato
de casca de romã, o qual apresentou atividade pró inflamatória. Todos os
tratamentos não foram capazes de reduzir de forma significativa o número de células
de C. albicans, enquanto para S. aureus todos os tratamentos apresentaram redução
significativa após quatorze dias de tratamento. Independente das nanopartículas de
prata serem produzidas quimicamente ou por meio do extrato da casca de romã, ambas
apresentaram considerável potencial de reparo de feridas infectadas em modelos in
vivo com ratos. Os achados das presentes pesquisas reforçam e estimulam o uso
potencial das nanopartículas de prata no tratamento de feridas, com destaque para a
síntese green por gerar menos danos ao meio ambiente e às pessoas envolvidas tanto
em sua produção como aos pacientes, e por apresentar, ainda, custo inferior quando
comparada às sínteses utilizando reagentes químicos e processos convencionais(AU)
The aim of this study was to investigate the production of silver nanoparticles
through peel extract of pomegranate, and produce formulations containing these
particles to be used in wound healings. Its antimicrobial action, cytotoxicity and
healing potential were tested. The synthesis of silver nanoparticles were based on
two methods proposed in the literature with some modifications, which were used
carboxymethylcellulose, propylene glycol, silver nitrate, water and peel extract of
pomegranate as reducing agent. The peel extract was characterized by pH, dry mass
and bioactive tannins (elagic acid and total phenols expressed as galic acid). The
total phenols were also quantified after being heated at 50ºC and 100ºC for 12
minutes, 1 hour and 2 hours. Then, silver nanoparticles were added in a solution
containing products to develop a formulation to be tested in wound healing. They
were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), zeta
potential and the quantification of remaining silver ions after the synthesis
reaction. The antimicrobial activity of the nanoparticles and formulations were
tested against Candida albicans (SC 5314) e Staphylococcus aureus (ATCC 25923) by
microdilution method. After submitting the peel extract to different conditions of
temperature and times (50ºC and 100ºC for 12 minutes, 1 hour and 2 hours), it was
noted that the values of the minimum inhibitory concentration was not affected and
were 391 µg/ml and 781 µg/ml for S. aureus and C. albicans. The formation of silver
nanoparticles was confirmed through the formation of characteristic peaks in the
UV-Vis spectrosc py and SEM images, and it was observed that the reaction at 50ºC
for 12 min produced silver nanoparticles with regular forms and better dispersed in
the formulation. The synthesis proposed promoted the reduction of silver ions at
about 100%, regardless of the time and temperature used in the reaction, which also
did not interfere in antimicrobial activity against C. albicans (68,75 µg/ml) and
S. aureus (67,50 µg/ml). After selecting the reaction at 50ºC for 12 min, the
silver nanoparticles produced ere also characterized by X-ray diffraction and
transmission electron microscopy (TEM), and the respective formulations through
dynamic light scattering, free ion dosage, zeta potential, SEM and TEM. The
stability test varying humidity and temperature was also performed for those
formulations. Besides antimicrobial assays, the cytotoxicity (L929 fibroblasts) of
the silver nanoparticles and the formulations of these particles and of the
pomegranate peel extract were evaluated. It was observed in the peel extract the
values of 3,13, 86,39±0,96% m/m, 3,64±0,03 mg/g and 392,0±9 mg/g respectively for
pH, dry mass, elagic acid and total phenols concentrations. Silver nanoparticles
produced by conventional chemical method was prepared and used as controls, and it
was noted the reduction potential of 99,89% and 99,51% for the synthesis using
pomegranate peel extract (AgNP green) and chemical reducing agent (AgNP chemical).
The averages sizes of green and chemical AgNP were 89 and 19 nm. The formulation
containing silver nanoparticles presented an antimicrobial potential expressively
higher than the active input, being 254 and 5- fold more effective against S.
aureus and C. albicans. Also, the cytotoxicity was notable reduced when silver
nanoparticles were produced using pomegranate peel extract. Based on the MIC values
and the cytotoxicity findings, the concentration of the formulations were
determined: i)
AgNP green at 337.5 µg/ml, ii) AgNP chemical at 5.55 µg/ml, and iii) pomegranate
peel extract at 94 µg/ml. In the in vivo study, a commercial form of silver
(Sulfadizsine) to the wound healing was used as control. Ninety Wistar male rats
were selected, and, after inducing diabetes, two incisions on the dorsum of the
animals were made and followed infected with S. aureus (ATCC 25923) and C. albicans
(SC 5314). After the infection, the animals were treated with the formulations
twice a day for 2, 7 and 14 days. Then, the animals were euthanized and the repair
potential was verified through wound closure index, inflammatory infiltrate
evaluation, angiogenesis, myeloperoxidase and hydroxyproline. It was also
determined the antimicrobial potential by counting the viable cells of each
microorganism used to infect the wounds. In general, the formulations containing
silver nanoparticles promoted a better closure of the wounds, and a higher anti-
inflamatory activity than the peel extract formulation which otherwise presented a
pro-inflamatory effect. All formulations could not significantly reduce the viable
cells of C. albicans, while for S. aureus they reduced significantly the cells
after 14 days of treatment. Silver nanoparticles produced by both green and
conventional chemical process present notable potential in repairing infected
wounds in in vivo rat model. The findings of the present research strengthen and
stimulate the potential application of silver nanoparticles in wound healings,
highlighting the green production of these particles which apart from being lower
costly, it is ecofriendly and less detrimental to people involved in its production
and use(AU)
AN - rayyan-553780453
AU - Fernandes, Renan Aparecido
KW - Biofilmes
Biofilms
Candida albicans
Nanotecnologia
Prata
Silver
Virulence nanotechnology
LA - en
PY - 2017
SP - 120-120
ST - Fitossíntese de nanopartículas de prata a partir de extrato de cascas de romã
e desenvolvimento de formulação para tratamento de feridas: avaliação
antimicrobiana, citotóxica e potencial cicatrizante em um modelo in vivo
TI - Fitossíntese de nanopartículas de prata a partir de extrato de cascas de romã
e desenvolvimento de formulação para tratamento de feridas: avaliação
antimicrobiana, citotóxica e potencial cicatrizante em um modelo in vivo
UR - https://repositorio.unesp.br/handle/11449/151982
ID - 8482
ER -
TY - JOUR
AB - Spondyloarthropathies are a group of chronic inflammatory disorders that
involve the joints of the axial skeleton, peripheral joints and have extra-
articular manifestations. Treatment includes inhibitors of tumor necrosis factor α.
Currently there are five approved inhibitors: a soluble receptor, Etanercept and
four monoclonal. Etanercept has very low toxicity with pulmonary adverse reactions
being very rare. We present the case of a patient who developed respiratory
symptoms and pulmonary infiltrates of rapid evolution after the third dose of
treatment with Etanercept. © 2020 The Authors
AN - rayyan-553780456
AU - Fernández-Trujillo, L.
AU - Iriarte, M. B.
AU - Puerta, G.
AU - Morales, E. I.
AU - Sua, L. F.
AU - Cañas, C. A.
DO - 10.1016/j.rmcr.2020.101079
KW - Anti-TNF
Case report
Etanercept
Pulmonary granulomatosis
Spondyloarthropathy
TNF-α
bilirubin
creatinine
etanercept
etoricoxib
hemoglobin
methylprednisolone
prednisone
vitamin D
adult
arthralgia
Article
bone marrow edema
bronchoscopy
case report
clinical article
coughing
crackle
cytology
disease association
dry cough
dyspnea
electrocardiogram
female
granulomatous pneumonitis
human
human cell
human tissue
laboratory test
lung lavage
myalgia
nuclear magnetic resonance imaging
pain
pneumonia
priority journal
provocation test
sacroiliitis
silver staining
spondyloarthropathy
transbronchial biopsy
transthoracic echocardiography
Spondylarthropathies
Pneumonia
PY - 2020
ST - Early instauration granulomatous pneumonitis associated with use of
etanercept in seronegative spondyloarthropathy: Case report
T2 - Respiratory Medicine Case Reports
TI - Early instauration granulomatous pneumonitis associated with use of
etanercept in seronegative spondyloarthropathy: Case report
85084975572&doi=10.1016%2fj.rmcr.2020.101079&partnerID=40&md5=eb63ce9a113008d0ff553
2bac226a3bd
VL - 30
ID - 8485
ER -
TY - JOUR
AB - Bacterial, protozoan and other microbial infections share an accelerated
metabolic rate. In order to ensure a proper functioning of cell replication and
proteins and nucleic acids synthesis processes, folate metabolism rate is also
increased in these cases. For this reason, folic acid antagonists have been used
since their discovery to treat different kinds of microbial infections, taking
advantage of this metabolic difference when compared with human cells. However,
resistances to these compounds have emerged since then and only combined therapies
are currently used in clinic. In addition, some of these compounds have been found
to have an immunomodulatory behavior that allows clinicians using them as anti-
inflammatory or immunosuppressive drugs. Therefore, the aim of this review is to
provide an updated state-of-the-art on the use of antifolates as antibacterial and
immunomodulating agents in the clinical setting, as well as to present their action
mechanisms and currently investigated biomedical applications. © 2019 by the
authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780457
AU - Fernández-Villa, D.
AU - Aguilar, M. R.
AU - Rojo, L.
DO - 10.3390/ijms20204996
IS - 20
KW - Antibacterials
Antibiotics
Antifolates
Antimalarial
Folic acid antagonists
Immunomodulation
Sulfonamides
Animals
Drug Resistance
Folic Acid Antagonists
Humans
Immunologic Factors
Structure-Activity Relationship
carbonate dehydratase
cotrimoxazole
dihydrofolate reductase
dihydropteroate synthase
endothelial nitric oxide synthase
endothelin 1
erythromycin
folic acid
folic acid antagonist
G protein coupled receptor
glutamate synthase
immunomodulating agent
interleukin 17
interleukin 8
mafenide acetate
n4 acetylsulfafurazole
sulfadiazine
sulfadiazine silver
sulfafurazole
sulfathiazole
thymidylate synthase
vancomycin
antiinfective agent
immunologic factor
apoptosis
cancer growth
CD4+ T lymphocyte
CD8+ T lymphocyte
nonhuman
prostatitis
pruritus
psoriasis
Review
toxoplasmosis
ulcerative colitis
wound healing
animal
chemistry
drug effect
drug resistance
human
immunomodulation
microbial sensitivity test
structure activity relation
Folic Acid
PY - 2019
ST - Folic acid antagonists: Antimicrobial and immunomodulating mechanisms and
applications
T2 - International Journal of Molecular Sciences
TI - Folic acid antagonists: Antimicrobial and immunomodulating mechanisms and
applications
85073095400&doi=10.3390%2fijms20204996&partnerID=40&md5=97b37543682d9443d70846dfd8e
6e490
VL - 20
ID - 8486
ER -
TY - GEN
AB - Este estudo teve como objetivo avaliar a biocompatibilidade, através de
análise histopatológica e de imuno-histoquímica, de um novo cimento reparador à
base de MTA com alta plasticidade: MTA HP (Angelus Londrina, PR). O MTA branco
(Angelus Londrina, PR), e um material a base de óxido de zinco e eugenol (IRM,
Dentsply, Petrópolis, RJ) foram utilizados como referências para comparação. Para
isso, trinta ratos machos de linhagem Wistar tiveram inoculados no tecido
subcutâneo um tubo de polietileno vazio (controle negativo) e mais três tubos, cada
um preenchido com um dos materiais testados. Os animais foram eutanasiados após 7,
30 e 60 dias da implantação dos tubos e as amostras foram fixadas e incluídas em
parafina. Os cortes histológicos foram corados com hematoxilina e eosina e
tricômico de gomori para avaliação das reações inflamatórias e a presença de
angiogênese foi realizada utilizando o marcador VEGF (do inglês vascular
endothelial growth factor). Os cortes também foram corados com Picrosirius Red para
quantificar as fibras colágenas do tipo I e tipo III, assim como a coloração de
Weigert foi realizada para observar as fibras elásticas. Os dados não-paramétricos
foram analisados usando o ensaio de Kruskal-Wallis seguido do teste de Dunn. Os
níveis de significância adotados foram de 5% (P < 0,05). Os resultados mostraram
diferença significativa da resposta inflamatória após 60 dias entre os grupos IRM e
tubo vazio (P < 0,05). O MTA HP apresentou biocompatibilidade similar ao MTA branco
e ao grupo controle negativo em todos os períodos experimentais. Além disso, após 7
dias o MTA HP estimulou a angiogênese de forma menos acentuada que o MTA branco,
assim como apresentou inicialmente um remodelamento mais lento da matriz
extracelular quando comparado ao MTA branco e o IRM. Foi observado uma diminuição
da espessura da cápsula fibrosa, da quantidade de fibras elásticas e da
imonumarcação com VEGF em todos os grupos experimentais e controle negativo ao
longo do processo de cicatrização. Após 60 dias os grupos experimentais
apresentaram matriz extracelular com tecido conjuntivo mais maduro, com
predominância de fibras colágenas do tipo I. De acordo com os resultados obtidos no
presente estudo, pode-se concluir que o novo cimento reparador com alta
plasticidade, MTA HP, apresentou-se biocompatível em todos os períodos
experimentais, com resultados similares aos grupos controle negativo e
experimentais com MTA branco e IRM. This study evaluated the biocompatibility,
through histopathological analysis and immunohistochemistry of a new repair cement
based on MTA with high plasticity: MTA HP (Angelus Londrina, PR). White MTA
(Angelus Londrina, PR), and a material based on zinc oxide and eugenol (IRM,
Dentsply, Petrópolis, RJ) were used as references for comparison. Thirty male
Wistar rats had inoculated into the subcutaneous tissue an empty polyethylene tube
(negative control) and three more tubes, each filled with one of the tested
materials. The animals were euthanized after 7, 30 and 60 days of tube implantation
and the specimens were fixed and embedded in paraffin. The sections were stained
with hematoxylin and eosin and gomori trichrome to assess inflammatory reactions,
and the presence of angiogenesis was performed using the VEGF (vascular endothelial
growth factor) marker. The sections were also stained with Picrosirius Red to
quantify as type I and type III collagen fibers, as well as a Weigert staining was
performed to observe elastic fibers. Non-parametric data were analyzed using the
Kruskal-Wallis assay followed Dunn's test. The significance levels adopted were 5%
(P < 0.05). The results demonstrated a significant difference in inflammatory
response after 60 days between IRM and empty tube groups (P < 0.05). MTA HP showed
similar biocompatibility to the White MTA and the negative control group in all
experimental periods. Furthermore, after 7 days MTA HP stimulated less pronounced
angiogenesis than White MTA, as it initially exhibited slower extracell lar matrix
remodeling when compared to White MTA and IRM. A decrease in the thickness of the
fibrous capsule, the amount of elastic fibers and the immunostaining with VEGF in
all experimental groups and control throughout the healing process was observed.
After 60 days, the experimental groups presented extracellular matrix with more
mature connective tissue, with predominance of type I collagen fibers. According to
the results obtained in the present study, it can be concluded that the new repair
cement with high plasticity, MTA HP, was biocompatible in all the experimental
periods, presenting similar results to the control and experimental groups with
White MTA and IRM.
AN - rayyan-553780458
AU - Ferreira, Cláudio Malizia Alves
KW - Agregado trióxido mineral
Biocompatibilidade
Biocompatibility
Imuno-histoquímica
Mineral trioxide aggregate
Subcutaneous tissue of rats
Tecido subcutâneo de ratos
LA - pt
PY - 2018
SP - 76-76
ST - Reação tecidual em subcutâneo de ratos frente a um cimento reparador à base
de MTA com alta plasticidade
TI - Reação tecidual em subcutâneo de ratos frente a um cimento reparador à base
de MTA com alta plasticidade
UR - http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=16327
ID - 8487
ER -
TY - JOUR
AB - The use of natural resources for the prevention and treatment of diseases
considered fatal to humanity has evolved. Several medicinal plants have nutritional
and pharmacological potential in the prevention and treatment of viral infections,
among them, turmeric, which is recognized for its biological properties associated
with curcuminoids, mainly represented by curcumin, and found mostly in rhizomes.
The purpose of this review was to compile the pharmacological activities of
curcumin and its analogs, aiming at stimulating their use as a therapeutic strategy
to treat infections caused by RNA genome viruses. We revisited its historical
application as an anti-inflammatory, antioxidant, and antiviral agent that combined
with low toxicity, motivated research against viruses affecting the population for
decades. Most findings concentrate particularly on arboviruses, HIV, and the recent
SARS-CoV-2. As one of the main conclusions, associating curcuminoids with
nanomaterials increases solubility, bioavailability, and antiviral effects,
characterized by blocking the entry of the virus into the cell or by inhibiting key
enzymes in viral replication and transcription.
AN - rayyan-553780459
AU - Ferreira, L. L. C.
AU - Abreu, M. P.
AU - Costa, C. B.
AU - Leda, P. O.
AU - Behrens, M. D.
AU - dos Santos, E. P.
DO - 10.1007/s12560-022-09514-3
IS - 2
KW - Genome
Genomics
PY - 2022
SN - 1867-0334 1867-0342
SP - 120-137
ST - Curcumin and Its Analogs as a Therapeutic Strategy in Infections Caused by
RNA Genome Viruses
T2 - FOOD AND ENVIRONMENTAL VIROLOGY
TI - Curcumin and Its Analogs as a Therapeutic Strategy in Infections Caused by
RNA Genome Viruses
VL - 14
Y2 - 6
ID - 8488
ER -
TY - JOUR
AB - The purpose of our study is to investigate the comparative effects of
materials based on silver and gold nanoparticles functionalized with polyphenols
from Cornus Mas extract (AgNPs-CM and AuNPs-CM) in vivo on experimental
inflammation. The nanoparticles were obtained at room temperature under UV
irradiation and were characterized by different methods: ultraviolet-visible
spectroscopy, transmission electron microscopy, X ray diffraction, Fourier
transform infrared spectroscopy and dynamic light scattering. The modulatory
effects of AgNPs-CM and AuNPs-CM on inflammation were quantified by oxidative
stress parameters, pro and anti-inflammatory cytokines levels and apoptosis
assessment at 2 h, 24 and 48 h after induction of inflammation with carrageenan in
the paw tissue of Wistar rats. Our results showed that silver and gold
nanoparticles only partial and for a short period have mobilized the antioxidant
defense mechanisms. In addition, they diminished inflammation and apoptosis in the
early stage while later, at 48 h, exerted an immunomodulatory effect, activated ERK
½ and induced apoptosis. The photoreduced silver and gold nanoparticles,
functionalized with natural compounds, modulated the inflammation in a similar
manner in the soft tissue injected with carrageenan. In order to decipher the
mechanisms involved in interactions of metallic nanoparticles with biological
systems and for a complete assessment of the risks and benefits of these products
in clinical practice long term studies are necessary.
AN - rayyan-553781985
AU - Filip, G. A.
AU - Moldovan, B.
AU - Baldea, I.
AU - Olteanu, D.
AU - Suharoschi, R.
AU - Decea, N.
AU - Cismaru, C. M.
AU - Gal, E.
AU - Cenariu, M.
AU - Clichici, S.
AU - David, L.
DO - 10.1016/j.jphotobiol.2018.12.006
J2 - J Photochem Photobiol B
KW - Animals
Apoptosis/drug effects
Cornus/*chemistry
Gold/chemistry
Green Chemistry Technology
Inflammation/*drug therapy/pathology
Metal Nanoparticles/*chemistry/therapeutic use
Plant Extracts/pharmacology/therapeutic use
Rats
Rats, Wistar
Silver/chemistry
*Ultraviolet Rays
Ultraviolet Rays
Inflammation
Fruit
LA - eng
N1 - Department of Physiology, Iuliu Hatieganu University of Medicine and
Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania. Electronic address:
gabriela.filip@umfcluj.ro.; Research Center for Advanced Chemical Analysis,
Instrumentation and Chemometrics (ANALYTICA), Faculty of Chemistry and Chemical
Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 400028, Cluj-Napoca,
Romania. Electronic address: bianca@chem.ubbcluj.ro.; Department of Physiology,
Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca,
Romania. Electronic address: baldeaioana@gmail.com.; Department of Physiology,
Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca,
Romania. Electronic address: ariana_di@yahoo.com.; University of Agricultural
Sciences and Veterinary Medicine, Faculty of Food Science and Technology, Calea
Manastur 3-5, 400372 Cluj-Napoca, Romania. Electronic address:
ramona.suharoschi@usamvcluj.ro.; Department of Physiology, Iuliu Hatieganu
University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania.
Electronic address: nicoleta.decea@yahoo.com.; Department of Infectious Diseases,
"Iuliu Hatieganu" University of Medicine and Pharmacy, 400348 Cluj-Napoca, Romania.
Electronic address: cristina.cismaru@umfcluj.ro.; Faculty of Chemistry and Chemical
Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca,
Romania. Electronic address: emese@chem.ubbcluj.ro.; University of Agricultural
Sciences and Veterinary Medicine, Department of Biochemistry, Calea Manastur 3-5,
400372 Cluj-Napoca, Romania. Electronic address: mihai.cenariu@usamvcluj.ro.;
Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy,
Clinicilor 1-3, Cluj-Napoca, Romania. Electronic address:
simonaclichici@yahoo.com.; Faculty of Chemistry and Chemical Engineering, Babeş-
Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, Romania. Electronic
address: muntean@chem.ubbcluj.ro.
PY - 2019
SP - 26-37
ST - UV-light mediated green synthesis of silver and gold nanoparticles using
Cornelian cherry fruit extract and their comparative effects in experimental
inflammation
T2 - Journal of photochemistry and photobiology. B, Biology
TI - UV-light mediated green synthesis of silver and gold nanoparticles using
Cornelian cherry fruit extract and their comparative effects in experimental
inflammation
VL - 191
Y2 - 2
ID - 9902
ER -
TY - JOUR
AB - Post-traumatic cystic cavitation, in which the size and severity of a CNS
injury progress from a small area of direct trauma to a greatly enlarged secondary
injury surrounded by glial scar tissue, is a poorly understood complication of
damage to the brain and spinal cord. Using minimally invasive techniques to avoid
primary physical injury, this study demonstrates in vivo that inflammatory
processes alone initiate a cascade of secondary tissue damage, progressive
cavitation, and glial scarring in the CNS. An in vitro model allowed us to test the
hypothesis that specific molecules that stimulate macrophage inflammatory
activation are an important step in initiating secondary neuropathology. Time-lapse
video analyses of inflammation-induced cavitation in our in vitro model revealed
that this process occurs primarily via a previously undescribed cellular mechanism
involving dramatic astrocyte morphological changes and rapid migration. The
physical process of cavitation leads to astrocyte abandonment of neuronal
processes, neurite stretching, and secondary injury. The macrophage mannose
receptor and the complement receptor type 3 beta2-integrin are implicated in the
cascade that induces cavity and scar formation. We also demonstrate that anti-
inflammatory agents modulating transcription via the nuclear hormone receptor
peroxisome proliferator-activated receptor-gamma may be therapeutic in preventing
progressive cavitation by limiting inflammation and subsequent secondary damage
after CNS injury.
AN - rayyan-553782199
AU - Fitch, M. T.
AU - Doller, C.
AU - Combs, C. K.
AU - Landreth, G. E.
AU - Silver, J.
DO - 10.1523/JNEUROSCI.19-19-08182.1999
IS - 19
J2 - J Neurosci
KW - Animals
Animals, Newborn
Astrocytes/*pathology/physiology/ultrastructure
Axons/pathology/ultrastructure
Brain Injuries/chemically induced/*pathology/physiopathology
Cell Movement
Cells, Cultured
Cerebral Cortex/*pathology
Ganglia, Spinal/injuries/*pathology/physiopathology
Glial Fibrillary Acidic Protein/analysis
Indomethacin/pharmacology
*Inflammation/chemically induced
Lipopolysaccharides/administration & dosage/toxicity
Macrophage Activation
Macrophages, Peritoneal/drug effects/physiology
Microscopy, Video
Neurites/pathology/physiology/ultrastructure
Neuroglia/*pathology/physiology/ultrastructure
Neurons/*pathology/physiology/ultrastructure
Proteoglycans/biosynthesis/genetics
Rats
Thiazoles/pharmacology
*Thiazolidinediones
Zymosan/administration & dosage/toxicity
Inflammation
LA - eng
N1 - Department of Neurosciences, Case Western Reserve University School of
Medicine, Cleveland, Ohio 44106, USA.
PY - 1999
SP - 8182-98
ST - Cellular and molecular mechanisms of glial scarring and progressive
cavitation: in vivo and in vitro analysis of inflammation-induced secondary injury
after CNS trauma
T2 - The Journal of neuroscience : the official journal of the Society for
Neuroscience
TI - Cellular and molecular mechanisms of glial scarring and progressive
cavitation: in vivo and in vitro analysis of inflammation-induced secondary injury
after CNS trauma
VL - 19
Y2 - 10 y3 - 1
ID - 10109
ER -
TY - JOUR
AB - Background: The present study aimed to evaluate the potential differences in
the biological effects of two types of spherical silver particles of 20 and 200 nm
(Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of 50
nm and length up to 50 μm, using a complex 3D model representative for the alveolar
barrier cultured at air-liquid interface (ALI). The alveolar model was exposed to
0.05, 0.5 and 5 μg/cm 2 of test compounds at ALI using a state-of-the-art exposure
system (Vitrocell™Cloud System). Endpoints related to the oxidative stress
induction, anti-oxidant defence mechanisms, pro-inflammatory responses and cellular
death were selected to evaluate the biocompatibility of silver particles and
nanowires (AgNMs) and to further ascribe particular biological effects to the
different morphologic properties between the three types of AgNMs evaluated.
Results: Significant cytotoxic effect was observed for all three types of AgNMs at
the highest tested doses. The increased mRNA levels of the pro-apoptotic gene CASP7
suggests that apoptosis may occur after exposure to AgNWs. All three types of AgNMs
increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of the metal-binding
anti-oxidant metallothioneins (MTs), with AgNWs being the most potent inducer. Even
though all types of AgNMs induced the nuclear translocation of NF-kB, only AgNWs
increased the mRNA level of pro-inflammatory mediators. The pro-inflammatory
response elicited by AgNWs was further confirmed by the increased secretion of the
10 evaluated interleukins. Conclusion: In the current study, we demonstrated that
the direct exposure of a complex tetra-culture alveolar model to different types of
AgNMs at ALI induces shape- and size-specific biological responses. From the three
AgNMs tested, AgNWs were the most potent in inducing biological alterations.
Starting from 50 ng/cm 2 , a dose representative for an acute exposure in a high
exposure occupational setting, AgNWs induced prominent changes indicative for a
pro-inflammatory response. Even though the acute responses towards a dose
representative for a full-lifetime exposure were also evaluated, chronic exposure
scenarios at low dose are still unquestionably needed to reveal the human health
impact of AgNMs during realistic conditions. © 2019 The Author(s).
AN - rayyan-553780466
AU - Fizeşan, I.
AU - Cambier, S.
AU - Moschini, E.
AU - Chary, A.
AU - Nelissen, I.
AU - Ziebel, J.
AU - Audinot, J. N.
AU - Wirtz, T.
AU - Kruszewski, M.
AU - Pop, A.
AU - Kiss, B.
AU - Serchi, T.
AU - Loghin, F.
AU - Gutleb, A. C.
DO - 10.1186/s12989-019-0297-1
IS - 1
KW - Air-liquid interface
Inflammation
Silver nanowires
Tetra-culture
Air Pollutants
Blood-Air Barrier
Cell Survival
Cells, Cultured
Cytokines
Endothelial Cells
Gene Expression
Humans
Metal Nanoparticles
Models, Biological
Nanowires
Oxidative Stress
Particle Size
Pulmonary Alveoli
Silver
caspase 7
cytokine
endothelial leukocyte adhesion molecule 1
heme oxygenase 1
intercellular adhesion molecule 1
interleukin 6
interleukin 8
messenger RNA
metallothionein
nanowire
silver nanoparticle
silver nanowire
transcription factor
unclassified drug
metal nanoparticle
silver
air liquid interface cell culture
alveolar barrier culture
apical compartment
apoptosis
art
Article
biocompatibility
CASP7 gene
cell culture
cell transfer
controlled study
cytokine release
cytotoxicity
death
defense mechanism
dose response
E SELECTIN gene
gene
gene expression
human
human cell
ICAM1 gene
IL 6 gene
IL 8 gene
IL6 gene
IL8 gene
in vitro study
inflammation
long term exposure
metabolic disorder
morphology
oxidative stress
particle size
priority journal
stress
VCAM1 gene
air pollutant
biological model
cell survival
coculture
drug effect
endothelium cell
genetics
immunology
lung alveolus
lung gas exchange
metabolism
PY - 2019
ST - In vitro exposure of a 3D-tetraculture representative for the alveolar
barrier at the air-liquid interface to silver particles and nanowires
TI - In vitro exposure of a 3D-tetraculture representative for the alveolar
85063801688&doi=10.1186%2fs12989-019-0297-
1&partnerID=40&md5=6a33bf587473bc58b6cff805634adb11
VL - 16
ID - 8494
ER -
TY - JOUR
AB - BackgroundThe present study aimed to evaluate the potential differences in
the biological effects of two types of spherical silver particles of 20 and 200nm
(Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of
50nm and length up to 50m, using a complex 3D model representative for the alveolar
0.05, 0.5 and 5g/cm(2) of test compounds at ALI using a state-of-the-art exposure
system (VitrocellCloud System). Endpoints related to the oxidative stress
different morphologic properties between the three types of AgNMs
evaluated.ResultsSignificant cytotoxic effect was observed for all three types of
AgNMs at the highest tested doses. The increased mRNA levels of the pro-apoptotic
gene CASP7 suggests that apoptosis may occur after exposure to AgNWs. All three
types of AgNMs increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of
the metal-binding anti-oxidant metallothioneins (MTs), with AgNWs being the most
potent inducer. Even though all types of AgNMs induced the nuclear translocation of
NF-kB, only AgNWs increased the mRNA level of pro-inflammatory mediators. The pro-
inflammatory response elicited by AgNWs was further confirmed by the increased
secretion of the 10 evaluated interleukins.ConclusionIn the current study, we
demonstrated that the direct exposure of a complex tetra-culture alveolar model to
different types of AgNMs at ALI induces shape- and size-specific biological
responses. From the three AgNMs tested, AgNWs were the most potent in inducing
biological alterations. Starting from 50ng/cm(2), a dose representative for an
acute exposure in a high exposure occupational setting, AgNWs induced prominent
changes indicative for a pro-inflammatory response. Even though the acute responses
towards a dose representative for a full-lifetime exposure were also evaluated,
chronic exposure scenarios at low dose are still unquestionably needed to reveal
the human health impact of AgNMs during realistic conditions.
AN - rayyan-553780467
AU - Fizesan, I.
AU - Cambier, S.
AU - Moschini, E.
AU - Chary, A.
AU - Nelissen, I.
AU - Ziebel, J.
AU - Audinot, J. N.
AU - Wirtz, T.
AU - Kruszewski, M.
AU - Pop, A.
AU - Kiss, B.
AU - Serchi, T.
AU - Loghin, F.
AU - Gutleb, A. C.
DO - 10.1186/s12989-019-0297-1
PY - 2019
SN - 1743-8977
T2 - PARTICLE AND FIBRE TOXICOLOGY
VL - 16
Y2 - 4 y3 - 2
ID - 8495
ER -
TY - JOUR
AB - BACKGROUND: The present study aimed to evaluate the potential differences in
the biological effects of two types of spherical silver particles of 20 and 200 nm
(Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of 50
nm and length up to 50 μm, using a complex 3D model representative for the alveolar
0.05, 0.5 and 5 μg/cm(2) of test compounds at ALI using a state-of-the-art exposure
system (Vitrocell™Cloud System). Endpoints related to the oxidative stress
different morphologic properties between the three types of AgNMs evaluated.
RESULTS: Significant cytotoxic effect was observed for all three types of AgNMs at
the highest tested doses. The increased mRNA levels of the pro-apoptotic gene CASP7
suggests that apoptosis may occur after exposure to AgNWs. All three types of AgNMs
increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of the metal-binding
anti-oxidant metallothioneins (MTs), with AgNWs being the most potent inducer. Even
though all types of AgNMs induced the nuclear translocation of NF-kB, only AgNWs
increased the mRNA level of pro-inflammatory mediators. The pro-inflammatory
response elicited by AgNWs was further confirmed by the increased secretion of the
10 evaluated interleukins. CONCLUSION: In the current study, we demonstrated that
the direct exposure of a complex tetra-culture alveolar model to different types of
AgNMs at ALI induces shape- and size-specific biological responses. From the three
AgNMs tested, AgNWs were the most potent in inducing biological alterations.
Starting from 50 ng/cm(2), a dose representative for an acute exposure in a high
exposure occupational setting, AgNWs induced prominent changes indicative for a
pro-inflammatory response. Even though the acute responses towards a dose
representative for a full-lifetime exposure were also evaluated, chronic exposure
scenarios at low dose are still unquestionably needed to reveal the human health
impact of AgNMs during realistic conditions.
AN - rayyan-553782120
AU - Fizeșan, I.
AU - Cambier, S.
AU - Moschini, E.
AU - Chary, A.
AU - Nelissen, I.
AU - Ziebel, J.
AU - Audinot, J. N.
AU - Wirtz, T.
AU - Kruszewski, M.
AU - Pop, A.
AU - Kiss, B.
AU - Serchi, T.
AU - Loghin, F.
AU - Gutleb, A. C.
DO - 10.1186/s12989-019-0297-1
IS - 1
J2 - Part Fibre Toxicol
KW - Air Pollutants
Blood-Air Barrier/*drug effects
Cells, Cultured
Cytokines/genetics
Endothelial Cells/*drug effects/immunology/metabolism
Gene Expression/drug effects
Humans
Metal Nanoparticles/*toxicity
*Models, Biological
Nanowires/*toxicity
Oxidative Stress/drug effects/genetics
Particle Size
Pulmonary Alveoli/*drug effects/immunology/metabolism
Silver/*toxicity
LA - eng
N1 - Toxicology Department, Faculty of Pharmacy, Iuliu Hațieganu University of
Medicine and Pharmacy, Cluj-Napoca, Romania.; Environmental Research and Innovation
(ERIN) Department, Luxembourg Institute of Science and Technology, Belvaux,
Luxembourg.; Environmental Research and Innovation (ERIN) Department, Luxembourg
Institute of Science and Technology, Belvaux, Luxembourg.; Environmental Research
and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology,
Belvaux, Luxembourg.; Health Unit, Flemish Institute for Technological Research
(VITO NV), Mol, Belgium.; Environmental Research and Innovation (ERIN) Department,
Luxembourg Institute of Science and Technology, Belvaux, Luxembourg.; Material
Research and Technology (MRT) Department, Luxembourg Institute of Science and
Technology, Belvaux, Luxembourg.; Material Research and Technology (MRT)
Department, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg.;
Faculty of Medicine, University of Information Technology and Management in
Rzeszow, Sucharskiego 2, Rzeszow, Poland.; Centre for Radiobiology and Biological
Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, Warszawa,
Poland.; Toxicology Department, Faculty of Pharmacy, Iuliu Hațieganu University of
Medicine and Pharmacy, Cluj-Napoca, Romania.; Toxicology Department, Faculty of
Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-Napoca,
Romania.; Environmental Research and Innovation (ERIN) Department, Luxembourg
Institute of Science and Technology, Belvaux, Luxembourg.; Toxicology Department,
Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-
Napoca, Romania.; Environmental Research and Innovation (ERIN) Department,
Luxembourg Institute of Science and Technology, Belvaux, Luxembourg.
arno.gutleb@list.lu.
PY - 2019
SP - 14
T2 - Particle and fibre toxicology
VL - 16
Y2 - 4 y3 - 2
ID - 10031
ER -
TY - JOUR
AB - Noble metal nanoparticles (NP) with intrinsic antiangiogenic, antibacterial,
and anti-inflammatory properties have great potential as potent chemotherapeutics,
due to their unique features, including plasmonic properties for application in
photothermal therapy, and their capability to slow down the migration/invasion
speed of cancer cells and then suppress metastasis. In this work, gold (Au), silver
(Ag), and palladium (Pd) NP were synthesized by a green redox chemistry method with
the reduction of the metal salt precursor with glucose in the presence of
polyvinylpyrrolidone (PVP) as stabilizing and capping agent. The physicochemical
properties of the PVP-capped NP were investigated by UV-visible (UV-vis) and
attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopies,
dynamic light scattering (DLS), and atomic force microscopy (AFM), to scrutinize
the optical features and the interface between the metal surface and the capping
polymer, the hydrodynamic size, and the morphology, respectively. Biophysical
studies with model cell membranes were carried out by using laser scanning confocal
microscopy (LSM) with fluorescence recovery after photobleaching (FRAP) and
fluorescence resonance energy transfer (FRET) techniques. To this purpose,
artificial cell membranes of supported lipid bilayers (SLBs) made with 1-palmitoyl-
2-oleoyl-sn-glycerol-3-phosphocholine (POPC) dye-labeled with 7-nitro-2-1,3-
benzoxadiazol-4-yl (NBD, FRET donor) and/or lissamine rhodamine B sulfonyl (Rh,
FRET acceptor) were prepared. Proof-of-work in vitro cellular experiments were
carried out with prostate cancer cells (PC-3 line) in terms of cytotoxicity, cell
migration (wound scratch assay), NP cellular uptake, and cytoskeleton actin
perturbation.
AN - rayyan-553780473
AU - Foti, A.
AU - Cali, L.
AU - Petralia, S.
AU - Satriano, C.
DO - 10.3390/nano13101624
IS - 10
PY - 2023
SN - 2079-4991
ST - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Study at the Hybrid Interface with Biomimetic Cell Membranes and In Vitro Cell
Models
T2 - NANOMATERIALS
TI - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Models
VL - 13
Y2 - 5 y3 - 12
ID - 8501
ER -
TY - JOUR
AB - Noble metal nanoparticles (NP) with intrinsic antiangiogenic, antibacterial,
and anti-inflammatory properties have great potential as potent chemotherapeutics,
due to their unique features, including plasmonic properties for application in
photothermal therapy, and their capability to slow down the migration/invasion
speed of cancer cells and then suppress metastasis. In this work, gold (Au), silver
(Ag), and palladium (Pd) NP were synthesized by a green redox chemistry method with
the reduction of the metal salt precursor with glucose in the presence of
polyvinylpyrrolidone (PVP) as stabilizing and capping agent. The physicochemical
properties of the PVP-capped NP were investigated by UV-visible (UV-vis) and
attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopies,
dynamic light scattering (DLS), and atomic force microscopy (AFM), to scrutinize
the optical features and the interface between the metal surface and the capping
polymer, the hydrodynamic size, and the morphology, respectively. Biophysical
studies with model cell membranes were carried out by using laser scanning confocal
microscopy (LSM) with fluorescence recovery after photobleaching (FRAP) and
fluorescence resonance energy transfer (FRET) techniques. To this purpose,
artificial cell membranes of supported lipid bilayers (SLBs) made with 1-palmitoyl-
2-oleoyl-sn-glycerol-3-phosphocholine (POPC) dye-labeled with 7-nitro-2-1,3-
benzoxadiazol-4-yl (NBD, FRET donor) and/or lissamine rhodamine B sulfonyl (Rh,
FRET acceptor) were prepared. Proof-of-work in vitro cellular experiments were
carried out with prostate cancer cells (PC-3 line) in terms of cytotoxicity, cell
migration (wound scratch assay), NP cellular uptake, and cytoskeleton actin
perturbation.
AN - rayyan-553782272
AU - Foti, A.
AU - Calì, L.
AU - Petralia, S.
AU - Satriano, C.
DO - 10.3390/nano13101624
IS - 10
J2 - Nanomaterials (Basel)
LA - eng
N1 - Nano Hybrid Biointerfaces Laboratory (NHBIL), Department of Chemical
Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.; Nano
Hybrid Biointerfaces Laboratory (NHBIL), Department of Chemical Sciences,
University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.; Department of
Drug and Health Sciences, University of Catania, Viale Andrea Doria 6, 95125
Catania, Italy.; Nano Hybrid Biointerfaces Laboratory (NHBIL), Department of
Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania,
Italy.
PY - 2023
SN - 2079-4991 (Print)
ST - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Models
T2 - Nanomaterials (Basel, Switzerland)
TI - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Models
VL - 13
Y2 - 5 y3 - 12
ID - 10182
ER -
TY - JOUR
AB - Recruitment of cells and mediators is altered during impaired wound healing,
thereby delaying this process. To overcome this problem, the correlation of wound
healing in older rats, and the impact of different molecular weight of hyaluronan
without silver nanoparticles; (low-HA1), (High-HA2), (Medium- HA3) and with silver
nanoparticles (High-HA4) is investigated. The superior HA were selected to be
further investigated onto diabetic wounds. Our results pointed to a marked
deficiency in wounds granulation in older rats, which was accompanied with
impairment of healing process. In older rats group treated with HA2 or HA4,
granulation and dermal construction were improved. Furthermore, the number of
pathogenic bacteria on wounds was declined throughout the first 24 h by HA2 and
HA4. The wound size in HA4-treated older rats was significantly smaller than that
in other HA1, HA2 or HA3-treated older ones. Also, diabetes impaired the level of
inflammatory cytokine, in diabetic model. On contrary, HA4 was found to normalize
the level of inflammatory cytokine, in the diabetic model. Furthermore, HA4 was
found to recover all oxidative and toxicity markers in diabetic models. This data
confirms the critical role of HA4 to improve granulation and inflammatory mediators
in impaired older and diabetic rat wound healing. © 2016 Elsevier B.V.
AN - rayyan-553780474
AU - Fouda, M. M. G.
AU - Abdel-Mohsen, A. M.
AU - Ebaid, H.
AU - Hassan, I.
AU - Al-Tamimi, J.
AU - Abdel-Rahman, R. M.
AU - Metwalli, A.
AU - Alhazza, I.
AU - Rady, A.
AU - El-Faham, A.
AU - Jancar, J.
DO - 10.1016/j.ijbiomac.2016.05.021
KW - Animal models
Hyaluronan
Wound healing
Animals
Humans
Hyaluronic Acid
Inflammation
Metal Nanoparticles
Molecular Weight
Rats
Silver
Wound Healing
cytokine
hyaluronic acid
silver nanoparticle
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
bacterial count
controlled study
diabetes mellitus
in vivo study
inflammation
male
molecular weight
nonhuman
oxidative stress
rat
tissue regeneration
wound
wound healing
wound healing impairment
animal
chemistry
complication
drug effects
human
microbiology
pathology
PY - 2016
SP - 582-591
ST - Wound healing of different molecular weight of hyaluronan; in-vivo study
TI - Wound healing of different molecular weight of hyaluronan; in-vivo study
84969262169&doi=10.1016%2fj.ijbiomac.2016.05.021&partnerID=40&md5=15662c9dc076c52ee
aa6852b5b45f854
VL - 89
ID - 8502
ER -
TY - JOUR
AB - Nitric oxide (NO) is important for the regulation of a number of diverse
biological processes, including vascular tone, neurotransmission, inflammatory cell
responsiveness, defence against invading pathogens and wound healing. Transition
metal exchanged zeolites are nanoporous materials with high-capacity storage
properties for gases such as NO. The NO stores are liberated upon contact with
aqueous environments, thereby making them ideal candidates for use in biological
and clinical settings. Here, we demonstrate the NO release capacity and powerful
bactericidal properties of a novel NO-storing Zn2+-exchanged zeolite material at a
50 wt.% composition in a polytetrafluoroethylene polymer. Further to our published
data showing the anti-thrombotic effects of a similar NO-loaded zeolite, this study
demonstrates the antibacterial properties of NO-releasing zeolites against
clinically relevant strains of bacteria, namely Gram-negative Pseudomonas
aeruginosa and Gram-positive methicillin-sensitive and methicillin-resistant
Staphylococcus aureus and Clostridium difficile. Thus our study highlights the
potential of NO-loaded zeolites as biocompatible medical device coatings with anti-
infective properties. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - rayyan-553780475
AU - Fox, S.
AU - Wilkinson, T. S.
AU - Wheatley, P. S.
AU - Xiao, B.
AU - Morris, R. E.
AU - Sutherland, A.
AU - Simpson, A. J.
AU - Barlow, P. G.
AU - Butler, A. R.
AU - Megson, I. L.
AU - Rossi, A. G.
DO - 10.1016/j.actbio.2009.10.038
IS - 4
PY - 2010
SN - 1742-7061
SP - 1515-1521
ST - NO-loaded Zn2+-exchanged zeolite materials: A potential bifunctional anti-
bacterial strategy
TI - NO-loaded Zn2+-exchanged zeolite materials: A potential bifunctional anti-
bacterial strategy
VL - 6
Y2 - 4
ID - 8503
ER -
TY - JOUR
AB - The nanoparticles are known to reduce toxicity, enhance bioactivity and
improve targeting cells. The metal and metal oxide nanoparticles such as gold,
silver, zinc oxide, zirconium oxide, copper oxide, copper sulfide, selenium,
hydroxyl apatite, and titanium oxide were used in several biomedical applica-tions
especially diagnosis of cancer, etc. The selenium nanoparticles (SeNPs) are
explored due toits unique characteristics and various known therapeutic benefits
such as anti bacterial, anti fungal and anti cancer activities. The selenium
nanoparticles actively involved in the free radical scavenging activity and anti
inflammatory activity. The antioxidant activities and reducing risk of cancer.In
this present study, Cynodondactylon or Bermuda grass mediated synthesis of selenium
nanoparticles and it was characterized using UV-vis spectrophotometer. And its
antioxidant effect was analysed using DPPH assay. The plant extract of
Cynodondactylon was prepared using distilled water and mixed with the sodium
selenite solution. The solution was kept in a magnetic stirrer at 100 RPM for
constant stirring while being observed for any colour change. The results showed
that there was a significant antioxidant activity and peak confirm the selenium
nanoparticles formation. Finally we conclude that the selenium nanoparticles can be
used in various drug designing aspects in future. © 2020 International Journal of
Research in Pharmaceutical Sciences. All rights reserved.
AN - rayyan-553780476
AU - Francis, T.
AU - Preejitha, V. B.
AU - Rajeshkumar, S.
DO - 10.26452/ijrps.v11i4.3301
IS - 4
KW - Antioxidant activity
Green synthesis
Selenium nanoparticles
ascorbic acid
sodium selenite
antifungal activity
arrow root
Article
biocompatibility
Cynodon dactylon
diabetic complication
drug development
liver cirrhosis
nephrotoxicity
Antioxidants
Selenium
PY - 2020
SP - 6221-6226
ST - Antioxidant activity of cynodon dactylon mediated selenium nanoparticles
T2 - International Journal of Research in Pharmaceutical Sciences
TI - Antioxidant activity of cynodon dactylon mediated selenium nanoparticles
85092203654&doi=10.26452%2fijrps.v11i4.3301&partnerID=40&md5=c00c41651bea474a784938
8a067e028b
VL - 11
ID - 8504
ER -
TY - JOUR
AB - Background: Nanoparticles are widely used in different technological fields,
one of which is medicine. Because of their antibacterial properties, silver
nanoparticles (AgNPs) are used in several types of wound dressings for the
treatment of burns and nonhealing wounds, but their influence on each component of
the wound-healing process remains unclear. In the present study, we evaluated the
effects of AgNPs on normal human dermal fibroblasts (NHDFs) and normal human
epidermal keratinocytes (NHEKs). Both cell types are important for wound healing,
including with regard to inflammation, proliferation and tissue remodeling. Each
phase of wound healing can be characterized by the secretion of cytokines,
chemokines and growth factors. Methods: The production of inflammatory parameters
(tumor necrosis factor alpha [TNF-alpha], interleukin-6 [IL-6], IL-8 and IL-12 and
cyclooxygenase-2 [COX-2]), angiogenesis parameters (vascular endothelial growth
factor [VEGF], granulocyte macrophage colony-stimulating factor) and matrix
metalloproteinases (MMP-1, MMP-2, MMP-3 and MMP-9) by NHDFs and NHEKs were examined
by ELISA or Western blot after 24 and 48 hours of incubation with AgNPs. Results:
We found that AgNPs decreased some inflammatory cytokines (TNF-alpha and IL-12) and
growth factors (VEGF) that were produced by NHDFs and NHEKs after 24 and 48 hours
and decreased the expression of COX-2 after 24 hours but only at the highest
concentration of AgNPs (25 parts per million). Conclusions: The results indicate
that NHEKs are more susceptible to the application of AgNPs than NHDFs, and AgNPs
may be useful for medical applications for the treatment of wounds.
AN - rayyan-553780477
AU - Frankova, J.
AU - Pivodova, V.
AU - Vagnerova, H.
AU - Juranova, J.
AU - Ulrichova, J.
DO - 10.5301/jabfm.5000268
IS - 2
KW - Wound Healing
Keratinocytes
Cells, Cultured
PY - 2016
SN - 2280-8000
SP - E137-E142
ST - Effects of silver nanoparticles on primary cell cultures of fibroblasts and
keratinocytes in a wound-healing model
T2 - JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS
TI - Effects of silver nanoparticles on primary cell cultures of fibroblasts and
keratinocytes in a wound-healing model
VL - 14
Y2 - 4
ID - 8505
ER -
TY - JOUR
AB - Non-steroidal anti-inflammatory drugs (NSAIDs) contribute to gastrointestinal
ulcer formation by inhibiting epithelial cell migration and mucosal restitution;
however, the drug-affected signaling pathways are poorly defined. We investigated
whether NSAID inhibition of intestinal epithelial migration is associated with
depletion of intracellular polyamines, depolarization of membrane potential (E(m))
and altered surface expression of K(+) channels. Epithelial cell migration in
response to the wounding of confluent IEC-6 and IEC-Cdx2 monolayers was reduced by
indomethacin (100 microM), phenylbutazone (100 microM) and NS-398 (100 microM) but
not by SC-560 (1 microM). NSAID-inhibition of intestinal cell migration was not
associated with depletion of intracellular polyamines. Treatment of IEC-6 and IEC-
Cdx2 cells with indomethacin, phenylbutazone and NS-398 induced significant
depolarization of E(m), whereas treatment with SC-560 had no effect on E(m). The
E(m) of IEC-Cdx2 cells was: -38.5+/-1.8 mV under control conditions; -35.9+/-1.6 mV
after treatment with SC-560; -18.8+/-1.2 mV after treatment with indomethacin; and
-23.7+/-1.4 mV after treatment with NS-398. Whereas SC-560 had no significant
effects on the total cellular expression of K(v)1.4 channel protein, indomethacin
and NS-398 decreased not only the total cellular expression of K(v)1.4, but also
the cell surface expression of both K(v)1.4 and K(v)1.6 channel subunits in IEC-
Cdx2. Both K(v)1.4 and K(v)1.6 channel proteins were immunoprecipitated by K(v)1.4
antibody from IEC-Cdx2 lysates, indicating that these subunits co-assemble to form
heteromeric K(v) channels. These results suggest that NSAID inhibition of
epithelial cell migration is independent of polyamine-depletion, and is associated
with depolarization of E(m) and decreased surface expression of heteromeric K(v)1
channels.
AN - rayyan-553782156
AU - Freeman, L. C.
AU - Narvaez, D. F.
AU - McCoy, A.
AU - von Stein, F. B.
AU - Young, S.
AU - Silver, K.
AU - Ganta, S.
AU - Koch, D.
AU - Hunter, R.
AU - Gilmour, R. F.
AU - Lillich, J. D.
DO - 10.1016/j.bcp.2007.03.030
IS - 1
J2 - Biochem Pharmacol
KW - Anti-Inflammatory Agents, Non-Steroidal/*toxicity
Cell Line
Cell Membrane/drug effects/metabolism
Cell Movement/drug effects/physiology
Humans
Indomethacin/toxicity
Intestinal Mucosa/*drug effects/metabolism
Membrane Potentials/*drug effects
Nitrobenzenes/toxicity
Patch-Clamp Techniques
Phenylbutazone/toxicity
Polyamines/analysis/metabolism
Shaker Superfamily of Potassium Channels/*drug effects/metabolism
Signal Transduction/drug effects/physiology
Spectrometry, Mass, Electrospray Ionization
Sulfonamides/toxicity
Wound Healing/*drug effects/physiology
Intestines
LA - eng
N1 - Department of Anatomy and Physiology, Kansas State University, Manhattan, KS
66506, United States.
PY - 2007
SN - 0006-2952 (Print)
SP - 74-85
ST - Depolarization and decreased surface expression of K+ channels contribute to
NSAID-inhibition of intestinal restitution
T2 - Biochemical pharmacology
TI - Depolarization and decreased surface expression of K+ channels contribute to
NSAID-inhibition of intestinal restitution
VL - 74
Y2 - 6 y3 - 30
ID - 10067
ER -
TY - CONF
AB - Biocompatibility is the ability of a material appropriate trigger a
biological response, when applied to the body, without causing a chronic
inflammatory reaction, foreign body reaction or toxicity, is related to the
interaction of the cell/biomaterial. A few materials, if any, are completely inert
from the physiological point of view since, most of the components with a variety
of potential toxic or irritating. In addition, chemical reactions during cure of
the material may also produce undesirable effects. In order to increase knowledge
about the characteristics and properties of materials and their interaction with
the biological environment, this study aimed, through literature review, guide and
inform didactically professionals and academics on the importance of
biocompatibility of restorative materials more direct use in dental practice:
silver amalgam, composite resins and glass ionomer cements. It was concluded that,
among the restorative materials studied, the glass ionomer cement showed the best
characteristics and properties that confirm its biocompatibility in dental
practice. © (2015) Trans Tech Publications, Switzerland.
AN - rayyan-553780479
AU - Freire, W. P.
AU - Fook, M. V. L.
AU - Barbosa, E. F.
AU - dos S. Araújo, C.
AU - Barbosa, R. C.
AU - Pinheiro, Í M. F.
DA - 2015
DO - 10.4028/www.scientific.net/MSF.805.19
Biomaterial
Restorative materials
Artificial organs
Biomaterials
Cements
Dental alloys
Dental materials
Glass
Ionomers
Biological environments
Biological response
Chronic inflammatory
Dental restorative materials
Foreign body reactions
Glass ionomer cement
Undesirable effects
Toxic materials
SP - 19-25
ST - Biocompatibility of dental restorative materials
T2 - Materials Science Forum
TI - Biocompatibility of dental restorative materials
84922222528&doi=10.4028%2fwww.scientific.net
%2fMSF.805.19&partnerID=40&md5=7ed8fafa2b1c7c15371cc8226f36b884
VL - 805
ID - 8507
ER -
TY - JOUR
AB - PURPOSE: To evaluate the effects of maternal remote ischemic preconditioning
(IPCr) in the colonic mucosa of newborn rats subjected to hypoxia and
reoxygenation. METHODS: Newborn Wistar rats were divided into three groups. Control
Group (CG), Hypoxia and Reoxygenation Group (HRG) and Remote Ischemic
Preconditioning Group (IPCrG). Hypoxia and reoxygenation was performed 2x per day,
with an interval of 6 hours, on the 1st, 2nd and 3rd days of life, with 10 minutes
of CO2 at 100%, followed by 10 minutes O2 at 100%(HRG/IPCrG). The maternal IPCr was
performed 24 hours before delivery by applying a rubber band tourniquet to the left
hind limb (IPCrG). Segments of the colon underwent histological (HE) and
immunohistochemical analysis for caspase-3 and COX - 2. RESULTS: The histological
findings showed no intestinal mucosal damage in the CG group and severe lesions in
HRG that was attenuated in the IPCrG (p<0.05). The expression of the apoptotic
cells was lower in the HRG group than in the CG and IPCrG. The COX-2 expression was
intense in HRG and attenuated in the IPCrG (p<0.05). CONCLUSIONS: Maternal IPCr
protected the colonic mucosa of newborn rats subjected to hypoxia and
reoxygenation, reducing the morphological alterations and inflammatory response. It
ameliorates the occurrence of apoptosis, keeping the physiological process of
renewal and regeneration in the epithelial lining of the colonic mucosa. .
AN - rayyan-553780480
AU - Freitas, Maria Andréia Lopes de
AU - Gomes, Rúdnei de Oliveira Luciano
AU - Soares, Bruno Leonardo de Freitas
AU - Artigiani Neto, Ricardo
AU - Montero, Edna Frasson de Souza
AU - Martins, José Luiz
DO - 10.1590/S0102-86502014000700005
IS - 7
KW - Apoptosis
Colon
Enterocolitis
Necrotizing
Rats
Infant, Newborn
Ischemic Preconditioning
Anoxia
LA - en
PY - 2014
SN - 0102-8650
SP - 438-444
ST - Effects of maternal ischemic preconditioning in the colon of newborn rats
submitted to hypoxia-reoxygenation insult
T2 - Acta cir. bras
TI - Effects of maternal ischemic preconditioning in the colon of newborn rats
submitted to hypoxia-reoxygenation insult
86502014000700438
VL - 29
Y2 - 7 y3 - 1
ID - 8508
ER -
TY - JOUR
AB - Background: Some of the views contrasting the beneficial and toxic effects of
antimicrobials upon wound healing remain controversial. Objective: To assess the
clinical relevance of histological findings following antimicrobial applications on
chronic leg ulcers. Method. The present study was performed in three parallel
groups of 17 patients suffering from at least 2 similar chronic leg ulcers.
Clinical planimetric assessments were performed before and after 3 and 6 weeks of
treatment using hydrocolloid dressings. In addition, 1 ulcer in each patient
received applications of povidone-iodine (PVP-I), silver sulfadiazine or
chlorhexidine digluconate. Histological examinations were made at inclusion and
after the 6-week therapy. Time to healing was also recorded. Results: At entry in
the study, fibroblasts, macrophages, neutrophils and vessels were abundant in the
ulcers. In addition, focal necrotizing vasculitis was related to the
microbiological load. Compared to the control lesions, both the healing rate and
time to healing of the leg ulcers showed a modest improvement at the sites
receiving silver sulfadiazine (2-7%) or chlorhexidine digluconate (-1 to 5%). By
contrast, PVP-I increased significantly the healing rate (4-18%, p<0.01), and time
to healing was reduced by 2-9 weeks (p<0.01). The 3 antimicrobials decreased the
bacterial density, and the vascular margination and migration of inflammatory
cells, thus abating the vasculitic changes. PVP-I applications did not alter the
microvessels and did not significantly reduce the density in dendrocytes and
fibroblasts. By contrast, both silver sulfadiazine and chorhexidine digluconate
appeared to alter the superficial microsvasculature including the dendrocyte
population. Conclusion: Although topical antimicrobials may apparently achieve
almost similar activity on the bacterial load inside chronic leg ulcers, the
toxicity upon host cells was different among these agents. PVP-I appeared to be an
efficient compound in these respects exhibiting a positive and relevant clinical
effect. Copyright (C) 2002 S. Karger AG, Basel.
AN - rayyan-553780482
AU - Fumal, I.
AU - Braham, C.
AU - Paquet, P.
AU - Pierard-Franchimont, C.
AU - Pierard, G. E.
DO - 10.1159/000057729
KW - Leg
PY - 2002
SN - 1018-8665 1421-9832
SP - 70-74
ST - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
impaired by a polymicrobial flora: A proof-of-concept study
T2 - DERMATOLOGY
TI - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
impaired by a polymicrobial flora: A proof-of-concept study
VL - 204
ID - 8510
ER -
TY - JOUR
AB - BACKGROUND: Some of the views contrasting the beneficial and toxic effects of
antimicrobials upon wound healing remain controversial. OBJECTIVE: To assess the
clinical relevance of histological findings following antimicrobial applications on
chronic leg ulcers. METHOD: The present study was performed in three parallel
groups of 17 patients suffering from at least 2 similar chronic leg ulcers.
Clinical planimetric assessments were performed before and after 3 and 6 weeks of
treatment using hydrocolloid dressings. In addition, 1 ulcer in each patient
received applications of povidone-iodine (PVP-I), silver sulfadiazine or
chlorhexidine digluconate. Histological examinations were made at inclusion and
after the 6-week therapy. Time to healing was also recorded. RESULTS: At entry in
the study, fibroblasts, macrophages, neutrophils and vessels were abundant in the
ulcers. In addition, focal necrotizing vasculitis was related to the
microbiological load. Compared to the control lesions, both the healing rate and
time to healing of the leg ulcers showed a modest improvement at the sites
receiving silver sulfadiazine (2-7%) or chlorhexidine digluconate (-1 to 5%). By
contrast, PVP-I increased significantly the healing rate (4-18%, p < 0.01), and
time to healing was reduced by 2-9 weeks (p < 0.01). The 3 antimicrobials decreased
the bacterial density, and the vascular margination and migration of inflammatory
cells, thus abating the vasculitic changes. PVP-I applications did not alter the
microvessels and did not significantly reduce the density in dendrocytes and
fibroblasts. By contrast, both silver sulfadiazine and chorhexidine digluconate
appeared to alter the superficial microsvasculature including the dendrocyte
population. CONCLUSION: Although topical antimicrobials may apparently achieve
almost similar activity on the bacterial load inside chronic leg ulcers, the
toxicity upon host cells was different among these agents. PVP-I appeared to be an
efficient compound in these respects exhibiting a positive and relevant clinical
effect.
AN - rayyan-553782339
AU - Fumal, I.
AU - Braham, C.
AU - Paquet, P.
AU - Piérard-Franchimont, C.
AU - Piérard, G. E.
DO - 10.1159/000057729
J2 - Dermatology
KW - Anti-Infective Agents, Local/adverse effects/*therapeutic use
Chlorhexidine/adverse effects/*analogs & derivatives/therapeutic use
Humans
Leg Ulcer/*drug therapy/*microbiology
Middle Aged
Povidone-Iodine/adverse effects/*therapeutic use
Silver Sulfadiazine/adverse effects/therapeutic use
Wound Healing/*drug effects
Leg
LA - eng
N1 - Department of Dermatopathology, University Medical Center, Liège, Belgium.
PY - 2002
SN - 1018-8665 (Print)
SP - 70-4
ST - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
impaired by a polymicrobial flora: a proof-of-concept study
T2 - Dermatology (Basel, Switzerland)
TI - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
impaired by a polymicrobial flora: a proof-of-concept study
VL - 204
ID - 10247
ER -
TY - JOUR
AB - Background: Disruption of bile acid (BA) homeostasis plays a key role in
intestinal inflammation. The gut-liver axis is the main site for the regulation of
BA synthesis and BA pool size via the combined action of the nuclear Farnesoid X
Receptor (FXR) and the enterokine Fibroblast Growth Factor 19 (FGF19). Increasing
evidence have linked derangement of BA metabolism with dysbiosis and mucosal
inflammation. Thus, here we aimed to investigate the potential action of an FGF19
analogue on intestinal microbiota and inflammation. Methods: A novel engineered
non-tumorigenic variant of the FGF19 protein, M52-WO 2016/0168219 was generated. WT
and FXRnull mice were injected with AAV-FGF19-M52 or the control AAV-GFP and
subjected to Sodium Dextran Sulphate-induced colitis. Findings: FGF19-M52 reduced
BA synthesis and pool size, modulated its composition and protected mice from
intestinal inflammation. These events were coupled with preservation of the
intestinal epithelial barrier integrity, inhibition of inflammatory immune response
and modulation of microbiota composition. Interestingly, FGF19-M52-driven systemic
and local anti-inflammatory activity was completely abolished in Farnesoid X
Receptor (FXR)null mice, thus underscoring the need of FXR to guarantee
enterocytes’ fitness and complement FGF19 anti-inflammatory activity. To provide a
translational perspective, we also show that circulating FGF19 levels are reduced
in patients with Crohn's disease. Interpretation: Reactivation of the FXR-FGF19
axis in a murine model of intestinal inflammation could bona fide provide positive
changes in BA metabolism with consequent reduction of intestinal inflammation and
modulation of microbiota. These results point to the therapeutic potential of FGF19
in the treatment of intestinal inflammation with concomitant derangement of BA
homeostasis. Funding: A. Moschetta is funded by MIUR-PRIN 2017 <- 2017J3E2W2;
Italian Association for Cancer Research (AIRC, IG 23239); Interreg V-A Greece-Italy
2014-2020-SILVER WELLBEING, MIS5003627; HDHL-INTIMIC EuJPI-FATMAL; MIUR PON “R&I”
2014-2020-ARS01_01220. No money has been paid by NGM Biopharmaceuticals or any
other agency to write this article. © 2020 The Author(s)
AN - rayyan-553780484
AU - Gadaleta, R. M.
AU - Garcia-Irigoyen, O.
AU - Cariello, M.
AU - Scialpi, N.
AU - Peres, C.
AU - Vetrano, S.
AU - Fiorino, G.
AU - Danese, S.
AU - Ko, B.
AU - Luo, J.
AU - Porru, E.
AU - Roda, A.
AU - Sabbà, C.
AU - Moschetta, A.
DO - 10.1016/j.ebiom.2020.102719
KW - Bile acids
DSS-colitis
Enterokine
Intestinal inflammation
Nuclear receptors
Animals
Bile Acids and Salts
Colitis, Ulcerative
Crohn Disease
Female
Fibroblast Growth Factors
Humans
Male
Mice
Mice, Inbred C57BL
Peptides
Receptors, Cytoplasmic and Nuclear
Recombinant Proteins
adeno associated virus vector
bile acid
chenodeoxycholic acid
cholesterol 7alpha monooxygenase
cholic acid
claudin 2
dextran sulfate
farnesoid X receptor
fibroblast growth factor 19
interleukin 1beta
interleukin 6
kruppel like factor 4
messenger RNA
occludin
RNA 16S
transcription factor Cdx2
cell receptor
farnesoid X-activated receptor
fibroblast growth factor
fibroblast growth factor 15, mouse
peptide
recombinant protein
Anaeroplasmataceae
animal experiment
animal model
animal tissue
Article
body weight loss
colitis
controlled study
Crohn disease
cytotoxicity
dysbiosis
electrospray
gene expression
goblet cell
high throughput sequencing
histology
homeostasis
human
image analysis
inflammation
intestine flora
intestine mucosa permeability
male
metagenomics
Mollicutes
mouse
nonhuman
priority journal
rectum hemorrhage
remission
RNA extraction
ulcer
animal
C57BL mouse
chemistry
female
genetics
metabolism
microbiology
ulcerative colitis
Fibroblasts
Inflammation
Intercellular Signaling Peptides and Proteins
PY - 2020
ST - Fibroblast Growth Factor 19 modulates intestinal microbiota and inflammation
in presence of Farnesoid X Receptor
T2 - EBioMedicine
TI - Fibroblast Growth Factor 19 modulates intestinal microbiota and inflammation
in presence of Farnesoid X Receptor
85082820119&doi=10.1016%2fj.ebiom.2020.102719&partnerID=40&md5=2010285f7f4ba2bc1bfb
0c9946c71770
VL - 54
ID - 8512
ER -
TY - JOUR
AB - Because of their antimicrobial properties, the use of silver nanoparticles
(AgNPs) is increasing fast in industry, food, and medicine. In the food industry,
nanoparticles are used in packaging to enable better conservation products such as
sensors to track their lifetime, and as food additives, such as anti-caking agents
and clarifying agents for fruit juices. Nanoemulsions, used to encapsulate, protect
and deliver additives are also actively developed. Nanomaterials in foods will be
ingested and passed through the digestive tract. Those incorporated in food
packaging may also be released unintentionally into food, ending up in the
gastrointestinal tract. It is therefore important to make a risk assessment of
nanomaterials to the consumer. Thus, exposure to AgNPs is increasing in quantity
and it is imperative to know their adverse effects in man. However, controversies
still remain with respect to their toxic effects and their mechanisms.
Understanding the toxic effects and the interactions of AgNPs with biological
systems is necessary to handle these nanoparticles and their use. They usually
generate reactive oxygen species resulting in increased pro-inflammatory reactions
and oxidative stress via intracellular signalling pathways. Here, we mainly focus
on the routes of exposure of AgNPs, toxic effects and the mechanisms underlying the
induced toxicity. © 2014 Elsevier Ltd.
AN - rayyan-553780485
AU - Gaillet, S.
AU - Rouanet, J. M.
DO - 10.1016/j.fct.2014.12.019
KW - Mechanisms of action
Oral exposure
Oxidative stress
Toxicity
Animals
Food Additives
Food Contamination
Humans
Metal Nanoparticles
Oxidative Stress
Silver
silver nanoparticle
food additive
metal nanoparticle
silver
apoptosis
excretion
exposure
food industry
gastrointestinal absorption
human
inflammation
ingestion
intracellular signaling
nonhuman
oxidative stress
Review
risk assessment
tissue distribution
toxicity
toxicokinetics
analysis
animal
drug effects
food contamination
metabolism
oral drug administration
PY - 2015
SP - 58-63
ST - Silver nanoparticles: Their potential toxic effects after oral exposure and
underlying mechanisms - A review
TI - Silver nanoparticles: Their potential toxic effects after oral exposure and
underlying mechanisms - A review
84921306344&doi=10.1016%2fj.fct.2014.12.019&partnerID=40&md5=1a1e9b461b5e87456d3e46
77f72fa305
VL - 77
ID - 8513
ER -
TY - JOUR
AB - Biomedical application of silver nanoparticles (AgNPs) has been rapidly
increasing. Owing to their strong antimicrobial activity, AgNPs are used in
dermatology in the treatment of wounds and burns. However, recent evidence for
their cytotoxicity gives rise to safety concerns. This study was undertaken as a
part of an ongoing programme in our laboratory to develop a topical agent for wound
healing. Here, we investigated the potential toxicity of AgNPs using normal human
dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) with the
aim of comparing the effects of AgNPs and ionic silver (Ag-I). Besides the effect
of AgNPs and Ag-I on cell viability, the inflammatory response and DNA damage in
AgNPs and Ag-l treated cells were examined. The results showed that Ag-I were
significantly more toxic than AgNPs both on NHDF and NHEK. Noncytotoxic
concentrations of AgNPs and Ag-I did not induce DNA strand breaks and did not
affect inflammatory markers, except for a transient increase in interleukin 6
levels in Ag-I treated NHDF. The results showed that AgNPs are more suitable for
the intended application as a topical agent for wound healing up to the
concentration 25 mu g/mL.
AN - rayyan-553780488
AU - Galandakova, A.
AU - Frankova, J.
AU - Ambrozova, N.
AU - Habartova, K.
AU - Pivodova, V.
AU - Zalesak, B.
AU - Safarova, K.
AU - Smekalova, M.
AU - Ulrichova, J.
DO - 10.1177/0960327115611969
IS - 9
KW - Humanities
Humanism
Humans
Fibroblasts
Keratinocytes
PY - 2016
SN - 0960-3271 1477-0903
SP - 946-957
ST - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
T2 - HUMAN & EXPERIMENTAL TOXICOLOGY
TI - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
VL - 35
Y2 - 9
ID - 8516
ER -
TY - JOUR
AB - Biomedical application of silver nanoparticles (AgNPs) has been rapidly
increasing. Owing to their strong antimicrobial activity, AgNPs are used in
dermatology in the treatment of wounds and burns. However, recent evidence for
their cytotoxicity gives rise to safety concerns. This study was undertaken as a
part of an ongoing programme in our laboratory to develop a topical agent for wound
healing. Here, we investigated the potential toxicity of AgNPs using normal human
dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) with the
aim of comparing the effects of AgNPs and ionic silver (Ag-I). Besides the effect
of AgNPs and Ag-I on cell viability, the inflammatory response and DNA damage in
AgNPs and Ag-I-treated cells were examined. The results showed that Ag-I were
significantly more toxic than AgNPs both on NHDF and NHEK. Non-cytotoxic
concentrations of AgNPs and Ag-I did not induce DNA strand breaks and did not
affect inflammatory markers, except for a transient increase in interleukin 6
levels in Ag-I-treated NHDF. The results showed that AgNPs are more suitable for
the intended application as a topical agent for wound healing up to the
concentration 25 µg/mL.
AN - rayyan-553781782
AU - Galandáková, A.
AU - Franková, J.
AU - Ambrožová, N.
AU - Habartová, K.
AU - Pivodová, V.
AU - Zálešák, B.
AU - Šafářová, K.
AU - Smékalová, M.
AU - Ulrichová, J.
DO - 10.1177/0960327115611969
IS - 9
J2 - Hum Exp Toxicol
KW - Anti-Infective Agents/chemistry/*toxicity
Cell Culture Techniques
Cells, Cultured
DNA Damage
Epidermis/drug effects/pathology
Fibroblasts/*drug effects/pathology
Humans
Keratinocytes/*drug effects/pathology
Skin/*drug effects/pathology
Surface Properties
Humanities
Humanism
Fibroblasts
Keratinocytes
LA - eng
N1 - Department of Medical Chemistry and Biochemistry, Faculty of Medicine and
Dentistry, Palacký University, Olomouc, Czech Republic Faculty of Medicine and
Dentistry, Institute of Molecular and Translational Medicine, Palacký University,
Olomouc, Czech Republic galandakova.a@seznam.cz.; Department of Medical Chemistry
and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc,
Czech Republic Faculty of Medicine and Dentistry, Institute of Molecular and
Translational Medicine, Palacký University, Olomouc, Czech Republic.; Department of
Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký
University, Olomouc, Czech Republic.; Department of Medical Chemistry and
Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech
Republic.; Department of Medical Chemistry and Biochemistry, Faculty of Medicine
and Dentistry, Palacký University, Olomouc, Czech Republic Faculty of Medicine and
Dentistry, Institute of Molecular and Translational Medicine, Palacký University,
Olomouc, Czech Republic.; Department of Plastic and Aesthetic Surgery, University
Hospital Olomouc, Olomouc, Czech Republic.; Faculty of Science, Regional Centre of
Advanced Technologies and Materials, Palacký University, Olomouc, Czech Republic.;
Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacký
University, Olomouc, Czech Republic.; Department of Medical Chemistry and
Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech
Republic Faculty of Medicine and Dentistry, Institute of Molecular and
Translational Medicine, Palacký University, Olomouc, Czech Republic.
PY - 2016
SP - 946-57
ST - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
T2 - Human & experimental toxicology
TI - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
VL - 35
Y2 - 9
ID - 9713
ER -
TY - JOUR
AB - Chronic wounds affect healthcare systems and increase the risk of infection.
Despite the clinical demand for improving healing, an effective and safe
therapeutic approach is still lacking. Here, we present a novel spatio-temporal-
varying adhesive microneedle (MN) patch encapsulated with mesenchymal stem cell-
derived exosomes (MSC-exos) and antibacterial Ag nanoparticles (AgNPs) for
improving wound healing. This MN patch is fabricated by replicating a predesigned
flexible template. Consisting of porous methacrylate gelatin hydrogels, the MN tips
can continuously deliver anti-inflammatory and pro-angiogenic MSC-exos to wound
beds, which can accelerate healing by improving cell functions, remodeling blood
vessels and restoring immune systems. Besides, the silk-fibroin-composed MN back
enables the patch to stick to the skin well and fix at the wounded site, thus
avoiding secondary bandage. By incorporating AgNPs in the MN back, the MN patch is
endowed with the ability to efficiently resist microbial contamination and further
benefits wound repair. Such a composite MN patch performs well in relieving
inflammation, promoting angiogenesis, and suppressing wound-infecting bacteria
during wound injury in diabetic rats, with no adverse effects observed. All the
features make this multifunctional MN patch potentially valuable for clinical
applications. © 2022
AN - rayyan-553780490
AU - Gan, J.
AU - Zhang, X.
AU - Ma, W.
AU - Zhao, Y.
AU - Sun, L.
DO - 10.1016/j.nantod.2022.101630
KW - Bioinspired
Microneedle
MSC-exosome
Patch
Wound healing
Adhesives
Blood vessels
Cell culture
Needles
Stem cells
adhesive agent
CD11b antigen
CD63 antigen
CD81 antigen
CD86 antigen
CD9 antigen
dimeticone
gelatin
hydrogel
interleukin 10
interleukin 6
methacrylic acid
silk fibroin
silver nanoparticle
vasculotropin
vasculotropin A
Antibacterials
Exosomes
Microneedle patches
Microneedles
Spatio-temporal variation
agar diffusion
angiogenesis
animal cell
animal experiment
animal model
Article
biocompatibility
blood vessel
controlled study
diabetic wound
drug cytotoxicity
elasticity
exosome
human
human cell
immunofluorescence
in vitro study
macrophage
male
microbial contamination
NIH 3T3 cell line
nonhuman
permeability
rat
RAW 264.7 cell line
Western blotting
wound healing
Wound Healing
PY - 2022
ST - Antibacterial, adhesive, and MSC exosomes encapsulated microneedles with
spatio-temporal variation functions for diabetic wound healing
T2 - Nano Today
TI - Antibacterial, adhesive, and MSC exosomes encapsulated microneedles with
spatio-temporal variation functions for diabetic wound healing
85139297839&doi=10.1016%2fj.nantod.2022.101630&partnerID=40&md5=d76ffaccc4a44b14f26
6db520be79a82
VL - 47
ID - 8518
ER -
TY - JOUR
AB - Nanoparticle preparations of heavy metals have attracted enormous scientific
and technological interest. Biologically produced nanoparticle preparations of
heavy metals are elaborately described in traditional texts and being widely
prescribed. The underlying interactions of nano preparations within the
physiological fluids are key feature to understand their biological impact. In this
perspective, we performed an experimental assessment of the toxicity potential of a
marketed metallic preparation named Vasant Kusumakar Ras (VKR), wherein different
heavy metals in composite form are reduced to nanoparticle size to produce the
desired effect in diabetes and its complications. VKR (50. mg/kg) was administered
to Albino Wistar rats rendered diabetic using streptozotocin (90. mg/kg) in 2 days
old neonates. Anti-hyperglycemic effect was observed with VKR along with increased
levels of plasma insulin. Renal variables including total proteins and albumin
along with glomerular filtration rate were found to improve biochemically. The
results were supplemented by effects on different inflammatory and growth factors
like TNF-α, nitric oxide, TGF-β and VEGF. However, the results observed in kidney
histopathology were not in accordance with the biochemical parameters. Inflammation
observed in kidney was confirmed by immunostaining metallothionein, which was due
to the accumulation of heavy metals. Furthermore, mercury accumulation in kidney
further confirmed by autometallography, which activated mononuclear phagocyte
system, which generated an immune response. This was further supported by increase
in the extent of apoptosis in kidney tissues. In conclusion, nanoparticle
preparations of heavy metals can be toxic to kidney if it is not regulated with
respect to its surface chemistry and dosage. © 2013 Elsevier GmbH.
AN - rayyan-553780492
AU - Gandhi, S.
AU - Srinivasan, B. P.
AU - Akarte, A. S.
DO - 10.1016/j.etp.2013.05.004
IS - 7
KW - Apoptosis
Autometallography
Diabetic nephropathy
Mercury
Metallothionein
Nephrin
Animals
DNA Fragmentation
Glomerular Filtration Rate
Insulin
Kidney
Medicine, Ayurvedic
Metal Nanoparticles
Metals, Heavy
Rats
Rats, Wistar
Rattus norvegicus
adiponectin
albumin
creatinine
cystatin C
erythropoietin
glucose transporter 2
glucose transporter 4
gold
heavy metal
insulin
iron
lead
mercury
nanoparticle
nephrin
nitric oxide
silver
tin
vasculotropin
albumin blood level
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
controlled study
diabetic nephropathy
experimental study
glomerulus filtration rate
histopathology
immune response
insulin blood level
kidney parenchyma
non insulin dependent diabetes mellitus
nonhuman
particle size
protein blood level
protein expression
rat
streptozocin diabetes
toxicity testing
Streptozocin
PY - 2013
SP - 1127-1135
ST - An experimental assessment of toxic potential of nanoparticle preparation of
heavy metals in streptozotocin induced diabetes
T2 - Experimental and Toxicologic Pathology
TI - An experimental assessment of toxic potential of nanoparticle preparation of
heavy metals in streptozotocin induced diabetes
84885855265&doi=10.1016%2fj.etp.2013.05.004&partnerID=40&md5=a356d49f243d3d96075952
f81d3e7ad2
VL - 65
ID - 8520
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been increasingly used in a variety of
consumer products over the last decades. However, their potential adverse effects
have not been fully understood. In a previous study, we characterized
transcriptomic changes in human induced pluripotent stem cell (iPSC)-derived
hepatocyte-like cells (HLCs) in response to AgNP exposure. Here, we report findings
of a follow-up proteomic study that evaluated alternations at the protein level in
the same cell after being exposed to 10 mu g/ml AgNPs for 24 h. In total, 6287
proteins were identified across two groups of samples (n = 3). Among these
proteins, 665 were found to be differentially regulated (fold change >= 1.25, p <
0.01) between the AgNP-treated group and the untreated control group, including 264
upregulated and 401 downregulated. Bioinformatics analysis of the proteomics data,
in side-by-side comparison to the transcriptomics data, confirms and substantiates
previous findings on AgNP-induced alterations in metabolism, oxidative stress,
inflammation, and potential association with cancer. A mechanism of action was
proposed based on these results. Collectively, the findings of the current
proteomic study are consistent with those of the previous transcriptomic study and
further demonstrate the usefulness of iPSC-derived HLCs as an in vitro model for
liver nanotoxicology.
AN - rayyan-553780497
AU - Gao, X. G.
AU - Li, R.
AU - Yourick, J. J.
AU - Sprando, R. L.
DO - 10.1016/j.tiv.2021.105274
KW - Humanities
Humanism
Humans
Transcriptome
Proteomics
PY - 2022
SN - 0887-2333 1879-3177
ST - Transcriptomic and proteomic responses of silver nanoparticles in hepatocyte-
like cells derived from human induced pluripotent stem cells
TI - Transcriptomic and proteomic responses of silver nanoparticles in hepatocyte-
like cells derived from human induced pluripotent stem cells
VL - 79
Y2 - 3
ID - 8525
ER -
TY - JOUR
AB - Replacement of missing teeth is an essential component of comprehensive
dental care for patients suffering of edentulism. A popular option is implant-
supported restorations. However, implant surfaces can become colonized with
polymicrobial biofilms containing Candida species that may compromise peri-implant
health. To prevent this, implant components may be treated with a variety of
coatings to create surfaces that either repel the attachment of viable
microorganisms or kill microorganisms on contact. These coatings may consist of
nanoparticles of pure elements (more commonly silver, copper, and zinc), sanitizing
agents and disinfectants (quaternary ammonium ions and chlorhexidine), antibiotics
(cefalotin, vancomycin, and gentamicin), or antimicrobial peptides (AMPs). AMPs in
bioactive coatings have a number of advantages. They elicit a protective action
against pathogens, inhibit the formation of biofilms, are less toxic to host
tissues, and do not prompt inflammatory responses. Furthermore, many of these
coatings may involve unique delivery systems to direct their antimicrobial capacity
against pathogens, but not commensals. Coatings may also contain multiple
antimicrobial substances to widen antimicrobial activity across multiple microbial
species. Here, we compiled relevant information about a variety of creative
approaches used to generate antimicrobial prosthetic surfaces in the oral cavity
with the purpose of facilitating implant integration and peri-implant tissue
health.
AN - rayyan-553782371
AU - Garaicoa, J. L.
AU - Bates, A. M.
AU - Avila-Ortiz, G.
AU - Brogden, K. A.
DO - 10.3390/microorganisms8081247
IS - 8
J2 - Microorganisms
LA - eng
N1 - Department of Restorative Dentistry, Oregon Health and Science University,
School of Dentistry, Portland, OR 97201, USA and Escuela de Odontología,
Universidad de Especialidades Espiritu Santo, Guayaquil 092301, Ecuador.;
Department of Human Oncology, University of Wisconsin School of Medicine and Public
Health, University of Wisconsin-Madison, Madison, WI 53705, USA.; Department of
Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242,
USA.; Department of Periodontics, College of Dentistry, The University of Iowa,
Iowa City, IA 52242, USA.; Iowa Institute for Oral Health Research, College of
Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
PY - 2020
SN - 2076-2607 (Print)
ST - Antimicrobial Prosthetic Surfaces in the Oral Cavity-A Perspective on
Creative Approaches
T2 - Microorganisms
TI - Antimicrobial Prosthetic Surfaces in the Oral Cavity-A Perspective on
Creative Approaches
VL - 8
Y2 - 8 y3 - 17
ID - 10278
ER -
TY - JOUR
AB - Nanotechnology is an emerging science involving the manipulation of matter on
the nanometer scale. Nanoparticles (NPs) are engineered structures with at least
one dimension of 100 nm or less. These materials are progressively being used for
commercial purposes and being incorporated into everyday manufactured articles at
an increasing rate. These products include consumer items such as pharmaceuticals,
cosmetics, food, food packaging, and household products, among others. The same
unique physical and chemical properties that make NPs so attractive may be
associated with their potentially hazardous effects on cells and tissues. Despite
the large benefit ensured from the application of nanotechnology, many issues
related to NP behavior and adverse effects are not fully understood or should be
examined anew. The traditional hypothesis that NPs exhibit different or additional
hazards due to their "nano" size has been challenged in recent years, and NP
categorization according to their properties and toxicity mechanism has been
proposed instead. Possible undesirable results of these capabilities are harmful
interactions with biological systems and the environment, with the potential to
generate toxicity. Both in vivo and in vitro studies have shown that NPs are
closely associated with toxicity by increasing intracellular reactive oxygen
species (ROS) levels, and/or the levels of pro-inflammatory mediators. This review
summarizes available data on NP toxicity in biological systems, with particular
focus on oxidative stress and inflammation as the main mechanisms that lead to
adverse health effects following NP exposure.
AN - rayyan-553780501
AU - Garces, M.
AU - Caceres, L.
AU - Chiappetta, D.
AU - Magnani, N.
AU - Evelson, P.
DO - 10.1039/d1nj01415c
IS - 32
PY - 2021
SN - 1144-0546 1369-9261
SP - 14328-14344
ST - Current understanding of nanoparticle toxicity mechanisms and interactions
with biological systems
TI - Current understanding of nanoparticle toxicity mechanisms and interactions
with biological systems
VL - 45
Y2 - 8 y3 - 28
ID - 8529
ER -
TY - JOUR
AB - Biomimetic nanomaterials (BNMs) are functional materials containing nanoscale
components and having structural and technological similarities to natural
(biogenic) prototypes. Despite the fact that biomimetic approaches in materials
technology have been used since the second half of the 20th century, BNMs are still
at the forefront of materials science. This review considered a general
classification of such nanomaterials according to the characteristic features of
natural analogues that are reproduced in the preparation of BNMs, including
biomimetic structure, biomimetic synthesis, and the inclusion of biogenic
components. BNMs containing magnetic, metal, or metal oxide organic and ceramic
structural elements (including their various combinations) were considered
separately. The BNMs under consideration were analyzed according to the declared
areas of application, which included tooth and bone reconstruction, magnetic and
infrared hyperthermia, chemo- and immunotherapy, the development of new drugs for
targeted therapy, antibacterial and anti-inflammatory therapy, and bioimaging. In
conclusion, the authors' point of view is given about the prospects for the
development of this scientific area associated with the use of native, genetically
modified, or completely artificial phospholipid membranes, which allow combining
the physicochemical and biological properties of biogenic prototypes with high
biocompatibility, economic availability, and scalability of fully synthetic
nanomaterials.
AN - rayyan-553780504
AU - Gareev, K. G.
AU - Grouzdev, D. S.
AU - Koziaeva, V. V.
AU - Sitkov, N. O.
AU - Gao, H. L.
AU - Zimina, T. M.
AU - Shevtsov, M.
DO - 10.3390/nano12142485
IS - 14
KW - Biomimetics
PY - 2022
SN - 2079-4991
ST - Biomimetic Nanomaterials: Diversity, Technology, and Biomedical Applications
T2 - NANOMATERIALS
TI - Biomimetic Nanomaterials: Diversity, Technology, and Biomedical Applications
VL - 12
Y2 - 7
ID - 8532
ER -
TY - JOUR
AB - The aim of this review is to evaluate the possibility of delivering a silver-
acid complex via a Trojan-horse mechanism for managing periodontits. We theroised
that the complex could be an effective treatment option for bacterial inflammatory
processes in the oral cavity. Searches were conducted using MEDLINE, Embase, Web of
Science Core Collection, and Goo-gle Scholar search engines. We also reviewed
several reference lists of the included studies or relevant reviews identified by
the search. By using Medical Subject Headings (MeSH) ter-minology, a comprehensive
search was performed for the following keywords: silver, folic acid, periodontitis,
macrophages, Trojan-horse mechanism, toxicity, and targeting. Using the keywords
mentioned earlier, we selected 110 articles and after appropriate elimination the
review was written based on 37 papers. Accordingly the we noted that silver isons
were an effective approach to kill oral pathogens. Secondly the Trojan-horse
mechanism. could be used by macrophages (as the Trojan horse) to deliver silver
ions in large quantities to the inflammatory focus to kill the periodontopathogens.
The Trojan-horse mechanism has never been described in the field of dentistry
before. The proposed novel approach using the principle of Trojan Horse delivery of
drugs/chemicals could be used to manage oral inflam-matory conditions. This method
can be used to supplement regular treatments. (c) 2022 The Authors. Published by
Elsevier Inc. on behalf of FDI World Dental Federation. This is an open access
article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-
nd/4.0/)
AN - rayyan-553780505
AU - Geczi, Z.
AU - Roth, I.
AU - Kohidai, Z.
AU - Kohidai, L.
AU - Mukaddam, K.
AU - Hermann, P.
AU - Vegh, D.
AU - Zelles, T.
DO - 10.1016/j.identj.2022.08.003
IS - 3
KW - Horses
Periodontics
PY - 2023
SN - 0020-6539 1875-595X
SP - 346-353
ST - The use of Trojan-horse drug delivery system in managing periodontitis
T2 - INTERNATIONAL DENTAL JOURNAL
TI - The use of Trojan-horse drug delivery system in managing periodontitis
VL - 73
Y2 - 6
ID - 8533
ER -
TY - JOUR
AB - Background: Leishmaniasis is an infectious disease caused by parasites of the
genus Leishmania and presents different clinical manifestations. The adverse
effects, immunosuppression and resistant strains associated with this disease
necessitate the development of new drugs. Nanoparticles have shown potential as
alternative antileishmanial drugs. We showed in a previous study the biosynthesis,
characterization and ideal concentration of a nanocomposite that promoted
leishmanicidal activity. In the present study, we conducted a specific analysis to
show the mechanism of action of AgNP-PVP-MA (silver nanoparticle–
polyvinylpyrrolidone-[meglumine antimoniate (Glucantime®)]) nanocomposite during
Leishmania amazonensis infection in vitro. Results: Through ultrastructural
analysis, we observed significant alterations, such as the presence of small
vesicles in the flagellar pocket and in the extracellular membrane, myelin-like
structure formation in the Golgi complex and mitochondria, flagellum and plasma
membrane rupture, and electrodense material deposition at the edges of the parasite
nucleus in both evolutive forms. Furthermore, the Leishmania parasite infection
index in macrophages decreased significantly after treatment, and nitric oxide and
reactive oxygen species production levels were determined. Additionally,
inflammatory, and pro-inflammatory cytokine and chemokine production levels were
evaluated. The IL-4, TNF-α and MIP-1α levels increased significantly, while the IL-
17 A level decreased significantly after treatment. Conclusions: Thus, we
demonstrate in this study that the AgNP-PVP-MA nanocomposite has leishmanial
potential, and the mechanism of action was demonstrated for the first time, showing
that this bioproduct seems to be a potential alternative treatment for
leishmaniasis. © 2021, The Author(s).
AN - rayyan-553780506
AU - Gélvez, A. P. C.
AU - Diniz Junior, J. A. P.
AU - Brígida, R. T. S. S.
AU - Rodrigues, A. P. D.
DO - 10.1186/s12866-021-02267-2
IS - 1
KW - AgNP-PVP-MA nanocomposite
Antileishmanial
Cytokine production
Leishmaniasis
Ultrastructural alterations
Animals
Antiprotozoal Agents
Cells, Cultured
In Vitro Techniques
Leishmania
Macrophages
Meglumine Antimoniate
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Nanocomposites
Povidone
Silver
amphotericin B
CD14 antigen
gamma interferon
interleukin 17
interleukin 1beta
interleukin 4
interleukin 6
macrophage inflammatory protein 1alpha
meglumine antimonate
nanocomposite
nanoparticle
nitric oxide
RANTES
silver nanoparticle
antiprotozoal agent
metal nanoparticle
povidone
silver
animal cell
animal experiment
Article
Aspergillus flavus
biological activity
biosynthesis
cell activation
cell membrane
cell structure
cell vacuole
controlled study
cytokine production
cytotoxicity
cytotoxicity assay
electron microscopy
flow cytometry
gene expression
immune response
Leishmania amazonensis
leishmaniasis
leishmanicidal activity
limit of detection
macrophage
membrane rupture
mitochondrion
morphological adaptation
mouse
multivesicular body
nonhuman
oxidative stress
oxygen consumption
phagocytosis
promastigote
protein expression
Trypanosoma cruzi
ultrastructural
animal
Bagg albino mouse
cell culture
chemistry
drug effect
drug therapy
in vitro study
parasitology
pharmacology
physiology
ultrastructure
PY - 2021
ST - AgNP-PVP-meglumine antimoniate nanocomposite reduces Leishmania amazonensis
infection in macrophages
T2 - BMC Microbiology
TI - AgNP-PVP-meglumine antimoniate nanocomposite reduces Leishmania amazonensis
infection in macrophages
85110045001&doi=10.1186%2fs12866-021-02267-
2&partnerID=40&md5=6c820eb6a685091175e8268bd81acee4
VL - 21
ID - 8534
ER -
TY - JOUR
AB - Activated macrophages can promote regeneration of CNS axons. However,
macrophages also release factors that kill neurons. These opposing functions are
likely induced simultaneously but are rarely considered together in the same
experimental preparation. A goal of this study was to unequivocally document the
concurrent neurotoxic and neuroregenerative potential of activated macrophages. To
do so, we quantified the length and magnitude of axon growth from enhanced green
fluorescent protein-expressing dorsal root ganglion (DRG) neurons transplanted into
the spinal cord in relationship to discrete foci of activated macrophages.
Macrophages were activated via intraspinal injections of zymosan, a potent
inflammatory stimulus known to increase axon growth and cause neurotoxicity. Using
this approach, a significant increase in axon growth up to macrophage foci was
evident. Within and adjacent to macrophages, DRG and spinal cord axons were
destroyed. Macrophage toxicity became more evident when zymosan was injected closer
to DRG soma. Under these conditions, DRG neurons were killed or their ability to
extend axons was dramatically impaired. The concurrent induction of pro-
regenerative and neurotoxic functions in zymosan-activated macrophages (ZAMs) was
confirmed in vitro using DRG and cortical neurons. Importantly, the ability of ZAMs
to stimulate axon growth was transient; prolonged exposure to factors produced by
ZAMs enhanced cell death and impaired axon growth in surviving neurons.
Lipopolysaccharide, another potent macrophage activator, elicited a florid
macrophage response, but without enhancing axon growth or notable toxicity.
Together, these data show that a single mode of activation endows macrophages with
the ability to simultaneously promote axon regeneration and cell killing.
AN - rayyan-553780507
AU - Gensel, J. C.
AU - Nakamura, S.
AU - Guan, Z.
AU - van Rooijen, N.
AU - Ankeny, D. P.
AU - Popovich, P. G.
DO - 10.1523/JNEUROSCI.3992-08.2009
IS - 12
KW - Macrophages
Regeneration
Axons
PY - 2009
SN - 0270-6474
SP - 3956-3968
ST - Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity
T2 - JOURNAL OF NEUROSCIENCE
TI - Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity
VL - 29
Y2 - 3 y3 - 25
ID - 8535
ER -
TY - JOUR
AB - Achillea millefolium L. is a medicinal herb with more than 100 antifungal and
antimicrobial active biological compounds. In the present study, A. millefolium
plants at the flowering stage were treated with different concentrations of methyl
jasmonate and silver nanoparticles. Treatments increased lipid peroxidation,
flavonoid content, and yield of essential oils (ca. 230 %), but reduced radical
scavenging capacity and anthocyanin contents of the plants. Cytotoxicity of the
extracts of treated plants against cancer HeLa cells was improved as well.
Production of certain antimicrobial isoprenoids, i.e., camphor was significantly
increased. Certain precious compounds such as allo-ocimene, germacrene, trans-
caryophyllene, and farnesol with antibacterial, antifungal, anti-inflammatory, and
anticancer effects were also induced just after elicitation of the plants with
silver nanoparticles and methyl jasmonate. The results suggested silver
nanoparticles as a novel elicitor in plant biotechnology as effective as methyl
jasmonate in order for production of desired secondary metabolites in A.
millefolium.
AN - rayyan-553780512
AU - Ghanati, F.
AU - Bakhtiarian, S.
AU - Parast, B. M.
AU - Behrooz, M. K.
DO - 10.13005/bbra/1287
IS - 2
KW - Achillea millefolium L.
Essential oil
Methyl jasmonate (MeJA)
Redox status
Silver nano particles (AgNPS)
Achillea millefolium
Dryobalanops
3 carene
Achillea millefolium extract
allo ocimene
anthocyanin
apigenin
bicyclogermacrene
camphene
camphor
carvyl acetate
caryophyllene
coumarin
drug metabolite
essential oil
farnesol
flavonoid
germacrene D
jasmonic acid methyl ester
junipene
limonene
luteolin
para cymene
para mentha 1,5,8 triene
plant medicinal product
sesquiterpene derivative
silver nanoparticle
terpinene
terpinolene
trans 3 carene 2 ol
unclassified drug
unindexed drug
valencene
Achillea
antifungal activity
Article
controlled study
drug cytotoxicity
drug isolation
female
flowering
HeLa cell line
human
human cell
lipid peroxidation
nanopharmaceutics
plant biotechnology
plant metabolism
PY - 2014
SP - 391-399
ST - Production of new active phytocompounds by Achillea millefolium L. after
elicitation with silver nanoparticles and methyl jasmonate
T2 - Biosciences Biotechnology Research Asia
TI - Production of new active phytocompounds by Achillea millefolium L. after
elicitation with silver nanoparticles and methyl jasmonate
84913592936&doi=10.13005%2fbbra
%2f1287&partnerID=40&md5=16788ffbf16641337424de4947fae540
VL - 11
ID - 8540
ER -
TY - JOUR
AB - Improvement in nanotechnology has identified promising silver nanoparticles
(AgNPs) for many biomedicine applications. To assess the toxicity of silver
nanoparticles in vivo, histopatological examinations of experimental mice were
studied. Forty adult male Sprague-Dawley rats were randomly divided into five
groups and orally treated AgNPs in different doses (30,125,300,700 mg/kg) during
the 28-days. After paraffin embedding and hematoxylin and eosin (H&E) and periodic
acid Schiff (PAS) staining, histopathological changes evaluated in kidney using
light micrographs. The obtained results showed a decrease in diameter of convoluted
tubules, glomerulus diameter, Bowman's space thickness and number of mesangeal
cells in 30 and 125 and 300 mg/kg treated groups. These changes are more evident in
125 mg/kg of AgNPs group (P<0.05). The other histological changes in the tubules of
rats affected by AgNPs included loose of brush border, basement membrane
irregularity, necrosis, vacuolar degeneration and Congestion. The other glomerular
and interstital alterations of rats affected by AgNPs included: basement membrane
thickness, accumulation of mesangial matrix, necrosis and infiltration of
inflammatory cells. These histological alterations were also more evident in rats
exposed to 125 mg/kg of AgNPs. In 700 mg/kg group, no major changes in the
structural component of the kidney were observed while occasional foci of
inflammatory cell infiltrates were present.
AN - rayyan-553780515
AU - Gherkhbolagh, M. H.
AU - Alizadeh, Z.
AU - Asari, M. J.
AU - Sohrabi, M.
DO - 10.24896/jrmds.2018619
IS - 1
KW - Administration, Oral
Rats
PY - 2018
SN - 2347-2545 2347-2367
SP - 43-51
ST - In Vivo Induced Nephrotoxicity of Silver Nanoparticles in Rat after Oral
Administration
T2 - JOURNAL OF RESEARCH IN MEDICAL AND DENTAL SCIENCE
TI - In Vivo Induced Nephrotoxicity of Silver Nanoparticles in Rat after Oral
Administration
VL - 6
Y2 - 2
ID - 8543
ER -
TY - JOUR
AB - Copper nanoparticles are widely utilized in a variety of applications,
including metal catalysts, semiconductors, heat transfer fluids in machine tools,
and even in antibacterial medications. Forty mature healthy Westar rats were
utilized in the current investigation and grouped randomly into four groups (n = 10
rats/group). Group I (G1) was kept as a control group, but G2, G3, and G4 were
intraperitoneally injected with CuO NPs with a dose (5 mg, 10 mg, 25 mg/kg body
weight/day) respectively for 9 days. Rats were sacrificed; then, the livers and
kidneys were dissected and subjected to histopathological and immunohistochemical
examination. Our findings of G2 and G3 revealed mild to moderate degenerative
changes within the hepatic parenchyma, moderate blood vessel congestions, glycogen
depletion, hemosiderosis, and microvesicular steatosis (fatty changes within the
hepatocytes). In addition, at the level of kidney, our examination clarified
moderate degenerations of the renal corpuscles and renal tubules with moderate
swelling and congestions of the glomerulus with moderate vacuolations in the renal
tubules lining epithelium. On the other hand, increasing the dose of CuO NPs, the
toxicity became more obvious, where the liver of G4 revealed severe necrosis of
hepatocytes with completely disorganizations of the hepatic rays, loss of the
hepatic architectures, severe steatosis, severe hemosiderosis, sinusoidal
dilatations with congestions, as well as severe fibrous tissue proliferation with
anti-inflammatory cell infiltrations specially around portal triad with hyperplasia
of bile duct. Meanwhile in kidney, G4 clarified severe necrosis and atrophy of the
renal corpuscles with severe damage of Bowman's capsule leading to completely
disorganization and loss of normal renal cortex architectures, severe congestion of
the glomerulus, severe necrosis of the renal tubules with damage and sloughing for
its lining epithelium, and severe hemorrhage between renal tubules. In addition,
severe and diffuse caspase 3 immunoreactivity were observed within the hepatic and
renal tissues of G4. The present investigation was concluded that the CuO NPs have
a potential toxicological effect on the hepatic and renal tissues that may affect
their functions.-->
AN - rayyan-553780516
AU - Ghonimi, W. A. M.
AU - Alferah, M. A. Z.
AU - Dahran, N.
AU - El-Shetry, E. S.
DO - 10.1007/s11356-022-21521-2
IS - 54
KW - Rats
Caspases
Copper
PY - 2022
SN - 0944-1344 1614-7499
SP - 81923-81937
ST - Hepatic and renal toxicity following the injection of copper oxide
nanoparticles (CuO NPs) in mature male Westar rats: histochemical and caspase 3
immunohistochemical reactivities
T2 - ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
TI - Hepatic and renal toxicity following the injection of copper oxide
nanoparticles (CuO NPs) in mature male Westar rats: histochemical and caspase 3
immunohistochemical reactivities
VL - 29
Y2 - 11
ID - 8544
ER -
TY - JOUR
AB - Nanoparticles (NPs) are being used in several industries worldwide and can
introduce into the human body through different exposure routes, including
inhalation, oral administration, intravenous injection, and intramuscular or
transdermal delivery. The present in vivo study aimed to evaluate the acute oral
toxicological effects of silica (SiO2) and magnesium oxide (MgO) NPs in rats by
using histological, biochemical, and biodistribution parameters. The results
revealed that acute exposure to higher doses of these NPs produced a significant
decrease (p < 0.05) in alanine aminotransferase, alkaline phosphatase serum levels
lactate dehydrogenase, and aspartate aminotransferase. Mild congestion, non-zonal
hepatocellular swelling and degeneration, and apoptotic cells with significant
pyknotic or shrunken nuclei were found in the liver of the treated rats at 2000
mg/kg of the MgO NPs. Moreover, under the microscopic examination, focal
hepatocellular degeneration and necrosis, accumulation of mononuclear inflammatory
cells within the necrotic area and in the portal tract, and severe central vein and
sinusoidal congestion and focal edematous fluids in the hepatic parenchyma were
observed in the livers of the treated rats with 2000 mg/kg of SiO2 NPs. Moreover,
MgO NPs exhibited higher liver and kidney accumulation than SiO2 NPs. In
conclusion, these NPs at a high concentration could have toxicological effects on
rats' liver and kidney tissues. However, further studies require examining the
safety and the other possible toxic effects of these NPs before entering the
consumer market.
AN - rayyan-553780517
AU - Ghorbani, S.
AU - Moshtaghi, H.
AU - Shekarforoush, S. S.
AU - Gheisari, H. R.
AU - Sedaghati, F.
AU - Nazifi, S.
AU - Ahmadi, N.
DO - 10.1007/s40995-023-01451-5
IS - 3
Rats
PY - 2023
SN - 2731-8095 2731-8109
SP - 695-705
ST - Histopathologic, Biochemical, and Biodistribution Studies of Orally
Administrated Silica and Magnesium Oxide Nanoparticles in Rats
T2 - IRANIAN JOURNAL OF SCIENCE
TI - Histopathologic, Biochemical, and Biodistribution Studies of Orally
Administrated Silica and Magnesium Oxide Nanoparticles in Rats
VL - 47
Y2 - 6
ID - 8545
ER -
TY - JOUR
AB - Background: Aegialitis rotundifolia Roxb., is a small mangrove tree or shrub
used traditionally as a potent cure for pain arising from various injuries.
Presently, there is a single scientific report on the wound healing property of
this mangrove species which has been performed in-vitro but there is no in vivo
scientific evidence of the wound healing properties. Further, few reports have
claimed that reduction of pain could accelerate wound healing process and thus, the
present work deals with the development of a topical ointment formulation
incorporated with Aegialitis rotundifolia Roxb., ethanolic leaves extract (ARELE)
which could potentially accelerate healing of excision, incision and burn wound
models in Wistar rats. Methods: Topical ointment containing ARELE was first tested
for their stability (90 days) and possible dermal toxicity using standard
procedures. In the excision wound model, parameters such as in-vivo antioxidant,
acute inflammatory marker (myeloperoxidase), wound microbial load, connective
tissue parameters, and histopathology of healed skin were performed. Incision and
burn (thermal and chemical) wounds were conducted following the standard methods.
Results: The ointment formulations were found to be stable and dermatologically
non-toxic. In the excision wound model, a significant increase in percent wound
contraction was observed for ARELE ointment treated groups which are substantiated
by strong in-vivo antioxidant activity, increased collagen formation, almost normal
skin histology, and reduced myeloperoxidase and microbial colonies. Strong wound
breaking strength was observed in incision wound repair and a significant increase
in percent wound closure in both thermal and chemical burn wound model was recorded
for ARELE ointment treated groups. Conclusion: Therefore, the topical application
of ARELE ointment formulations showed remarkable excision, incision and burn wound
healing in Wistar rats thus showing its potential as a promising wound healing
agent. © 2019 Elsevier GmbH
AN - rayyan-553780518
AU - Ghosh, D.
AU - Mondal, S.
AU - Ramakrishna, K.
DO - 10.1016/j.wndm.2019.100168
IS - 1
KW - Aegialitis rotundifolia
Burn wound
In-vivo antioxidant activity
Mangrove
Ointment formulation
Wound healing
Aegialitis rotundifolia extract
alcohol
alkaloid
carbohydrate
flavonoid
myeloperoxidase
phytosterol
plant extract
povidone iodine
saponin
sulfadiazine silver
tannin derivative
triterpenoid
unclassified drug
adult
animal experiment
animal model
animal tissue
Article
breaking strength
burn
chemical burn
collagen synthesis
connective tissue
controlled study
drug formulation
drug screening
drug stability
epithelization
histopathology
in vivo study
male
mangrove
nonhuman
ointment
pathogen load
plant leaf
priority journal
rat
skin irritation
skin toxicity
surgical wound
wound closure
wound contraction
wound healing
Rats
Wound Healing
Ointments
Burns
PY - 2019
ST - A topical ointment formulation containing leaves extract of Aegialitis
rotundifolia Roxb., accelerates excision, incision and burn wound healing in rats
T2 - Wound Medicine
TI - A topical ointment formulation containing leaves extract of Aegialitis
rotundifolia Roxb., accelerates excision, incision and burn wound healing in rats
85070057071&doi=10.1016%2fj.wndm.2019.100168&partnerID=40&md5=91c57444bc61a660ac399
2b83125b213
VL - 26
ID - 8546
ER -
TY - JOUR
AB - Wound dressing developed using bioactive materials has been a current area of
research for treating chronic non healing wounds owing to its high demand. Here, we
report the fabrication and evaluation of nanofibrous matrix based wound dressings
using biopolymer poly(vinyl alcohol) (PVA) incorporated with silk sericin (SS). SS
extracted from the cocoons of mulberry variety Botnbyx mori and non-mulberry
variety Antheraea assama has been used to develop two types of blended mats.
Herein, SS based nanofibrous dressings fabricated using electro-spinning technique
were thoroughly characterized and evaluated for wound healing applications. The
developed SS based nanofibrous mats ranged from 130 to 160 nm in diameter with
micro to nanoporous structure. The dressings were endowed with free radical
scavenging capacity, antibacterial activity, swelling capacity, and
biocompatibility due to incorporation of SS. Furthermore, murine fibroblasts (L929)
and human keratinocytes (HaCaT) cultured on the PVA-SS blended mats showed higher
proliferation as compared to pristine PVA mats as observed over a period of 14 days
(p <= 0.01). The blended mats also showed spread out morphology of cells in
comparison to spherical clumps formed on PVA mats. In addition, SS from both silk
types exhibited excellent antioxidant potential without hampering the cell
viability even under H2O2 driven oxidative stress. Moreover, SS (both types)
released from the nanofibrous mats also healed the wounds at thrice the rate of
control under in vitro conditions. Furthermore, subcutaneous implantation of
nanofibrous mats in mice showed in vivo tolerance of the blended nanofibrous mats
observed over four weeks without eliciting any inflammatory reactions to the host
tissue. Taken together, the developed silk sericin-based dressings signify an
attractive substrate for treatment of chronic wounds like diabetic foot ulcers.
AN - rayyan-553780521
AU - Gilotra, S.
AU - Chouhan, D.
AU - Bhardwaj, N.
AU - Nandi, S. K.
AU - Mandal, B. B.
DO - 10.1016/j.msec.2018.04.077
KW - Bandages
PY - 2018
SN - 0928-4931 1873-0191
SP - 420-432
ST - Potential of silk sericin based nanofibrous mats for wound dressing
applications
TI - Potential of silk sericin based nanofibrous mats for wound dressing
applications
VL - 90
Y2 - 9 y3 - 1
ID - 8549
ER -
TY - JOUR
AB - Although gold nanoparticles (AuNPs) are currently used in several industrial
products and biomedical applications, information about their biological effects is
very limited. Thus, it is becoming crucial to assess their safety and adequately
investigate the complexity of cell-nanoparticles interactions. In this work, the
Balb/3T3 mouse fibroblast cell line was selected as an in vitro model to study the
effects of AuNPs. Alteration of cellular processes and biochemical pathways caused
by AuNPs exposure was investigated by analysing the differentially expressed
proteome. Of interest was the difference observed in the protein pattern expression
of cells exposed to AuNPs. It was found that 88 and 83 proteins were de-regulated
after exposure to 5 and 15 nm AuNPs, respectively. Analysis of the proteome
revealed that AuNPs triggers several pathways related to cellular growth and
proliferation, cell morphology, cell cycle regulation, cellular function and
maintenance, oxidative stress, and inflammatory response. Moreover, SPR analysis
showed an increase of ECM proteins biosynthesis in cells exposed to AuNPs. We
observed by TEM analysis that NPs are internalized and confined mainly in
autophagosomes. Endoplasmic reticulum stressed and modification at mitochondrial
level occurred. This study aims to improve existing knowledge necessary for a
correct assessment of the balance between AuNPs potential adverse and beneficial
effects and might have important implications for biomedical applications (e.g.
nanomedicine). To conclude proteomics link to system biology analysis is a valuable
tool to understand and predict nanoparticles' toxicity, furthermore it has the
potential to reveal pathways that may not be immediately evident with classical
toxicological assays. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.
AN - rayyan-553780522
AU - Gioria, S.
AU - Chassaigne, H.
AU - Carpi, D.
AU - Parracino, A.
AU - Meschini, S.
AU - Barboro, P.
AU - Rossi, F.
DO - 10.1016/j.toxlet.2014.04.016
IS - 2
KW - Proteomics
Proteome
PY - 2014
SN - 0378-4274 1879-3169
SP - 111-126
ST - A proteomic approach to investigate AuNPs effects in Balb/3T3 cells
TI - A proteomic approach to investigate AuNPs effects in Balb/3T3 cells
VL - 228
Y2 - 7 y3 - 15
ID - 8550
ER -
TY - JOUR
AB - The aims of this study were to evaluate the ratio between inflammatory
reactions induced by four endodontic sealers and the occurrence of fibrosis and the
number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA).
Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus,
Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar
rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8
animals were euthanized, providing 6 specimens per test group and 2 specimens from
the control group. The fragments were subjected to histological processing and
immunohistochemical analysis for anti α-SMA protein. All specimens, except those
from the control group, presented severe inflammatory reaction on the 7th
postoperative day, which also coincided with a large number of myofibroblasts. On
the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill,
AH Plus and Acroseal decreased significantly, which coincided with reduced presence
of myofibroblasts and usual collagen deposition. In contrast, in the group filled
with Epiphany, significant inflammatory cell infiltrate was present in all analyzed
periods. The persistence of an inflammatory reaction induced by endodontic sealer
may also induce the development of fibrosis in combination with presence of
myofibroblasts. O objetivo deste estudo foi avaliar a relação entre reação
inflamatória induzida por quatro cimentos endodônticos e a presença de fibrose e
quantidade de miofibroblastos que apresentam positividade para α-SMA. Tubos de
polietileno foram preenchidos com o cimento (I: Endofill; II: AH Plus; III:
Acroseal; IV: Epiphany) e inseridos em 4 regiões do dorso de 24 ratos Wistar,
enquanto 2 tubos vazios (V - controle) foram inseridos em 6 ratos. Após 7, 21 e 45
dias, oito animais foram sacrificados obtendo 6 indivíduos por grupo e 2 para o
grupo controle. Os fragmentos foram submetidos ao processamento histológico e à
análise imuno-histoquímica para a proteína anti-α-SMA. Todos os grupos, exceto o
controle, demonstraram notável reação inflamatória no 7º dia pós-operatório, que
também coincidiu com uma grande quantidade de miofibroblastos. No 21º e 45º dia
pós-operatório, a reação inflamatória induzida pelo Endofill, AH Plus e Acroseal
diminuiu significativamente, o que coincidiu com reduzida presença de
miofibroblastos e deposição de colágeno normal. Em contraste, no grupo Epiphany,
infiltrado inflamatório significativo esteve presente em todos os períodos
analisados. A persistência do infiltrado inflamatório induzido por cimento
endodôntico pode também provocar uma fibrose associada com a presença de
miofibroblastos.
AN - rayyan-553780523
AU - Giovanini, Allan Fernando
AU - Leonardi, Denise Piotto
AU - Baratto-Filho, Flares
AU - Valença, Paola Cristine
AU - Moresca, Ricardo César
AU - Moro, Alexandre
AU - Schramm, Celso Alfredo
DO - 10.1590/S0103-64402011000500004
IS - 5
Endodontic sealers
Fibrosis
Myofibroblasts
LA - en
PY - 2011
SN - 0103-6440
SP - 369-376
ST - An endodontic sealer induces a pathological condition when associated with
persistent tissue toxicity and presence of myofibroblasts
T2 - Braz. dent. j
TI - An endodontic sealer induces a pathological condition when associated with
persistent tissue toxicity and presence of myofibroblasts
64402011000500004
VL - 22
ID - 8551
ER -
TY - JOUR
AB - The recent widespread applications of nanomaterials, because of their
properties, opens new scenarios that affect their dispersal in the environment. In
particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be
harmful for animals and humans. To evaluate possible toxic effects caused by carbon
nanotube environmental dispersion, with regard to aquatic compartment, we proposed
as experimental model a freshwater invertebrate: Hirudo medicinalis. In the present
study we analyse acute and chronic immune responses over a short (1, 3, 6 and 12
hours) and long time (from 1 to 5 weeks) exposure to MWCNTs by optical, electron
and immunohistochemical approaches. In the exposed leeches angiogenesis and
fibroplasia accompanied by massive cellular migration occur. Immunocytochemical
characterization using specific markers shows that in these inflammatory processes
the monocyte-macrophages (CD45(+), CD68(+)) are the most involved cells. These
immunocompetent cells are characterized by sequence of events starting from the
expression of pro-inflammatory cytokines (in particular IL-18), and
amyloidogenensis. Our combined experimental approaches, basing on high sensitive
inflammatory response can highlight adverse effects of nanomaterials on aquatic
organisms and could be useful to assess the MWCNTs impact on aquatic, terrestrial
animal and human health.
AN - rayyan-553780526
AU - Girardello, R.
AU - Tasselli, S.
AU - Baranzini, N.
AU - Valvassori, R.
AU - de Eguileor, M.
AU - Grimaldi, A.
DO - 10.1371/journal.pone.0144361
IS - 12
PY - 2015
SN - 1932-6203
ST - Effects of Carbon Nanotube Environmental Dispersion on an Aquatic
Invertebrate, Hirudo medicinalis
T2 - PLOS ONE
TI - Effects of Carbon Nanotube Environmental Dispersion on an Aquatic
Invertebrate, Hirudo medicinalis
VL - 10
Y2 - 12 y3 - 4
ID - 8554
ER -
TY - JOUR
AB - The aim of the study is to evaluate the therapeutic applications of silver
nanoparticles synthesized using 2 medicinal plants collected from yelagiri hills.
The leaves of the plants were used for optimization of silver nanoparticles by
varying the time exposure of the reaction mixture to sunlight (5, 10, 15 minutes).
The anti-oxidant, anti inflammatory and antimicrobial potentials of samples was
studied by different assays. Also, the synthesized nanoparticles were characterized
by UV, SEM, XRD and FTIR techniques. The results suggest that nanoparticles
synthesis was significant at exposure time of 5 and 10minutes. The synthesized
particles were confirmed by UV spectroscopy, which showed a characteristic peak at
427 and 418nm for the 2 samples respectively. The synthesized nanoparticles were
found to be in the size range of 60-80nm and possessed characteristic XRD peaks.
The results of the study revealed that the synthesized silver nanoparticles
possessed significant antioxidant, anti inflammatory, anti-proliferative and
antimicrobial properties.
AN - rayyan-553780527
AU - Giridharan, T.
AU - Chandran, M.
AU - Sindhu, S.
AU - Arumugam, P.
IS - 4
KW - Flacourtia sepiaria
FTIR
MCF7 cells
Rhinacanthus nasutus
SEM
XRD
Flacourtia
1,1 diphenyl 2 picrylhydrazyl
acetylsalicylic acid
ascorbic acid
cefotaxime
doxorubicin
Flacourtia sepiaria extract
free radical
hydroxyl radical
plant extract
Rhinacanthus nasutus extract
silver nanoparticle
tocopherol
unclassified drug
antifungal activity
Article
Bacillus subtilis
cancer cell culture
Candida albicans
Candida tropicalis
controlled study
drug cytotoxicity
Escherichia coli
green synthesis
human
human cell
liver cancer
MCF 7 cell line
medicinal plant
nonhuman
particle size
plant leaf
sun exposure
synthesis
X ray diffraction
PY - 2014
SP - B560-B569
ST - Comparative studies on green synthesis and therapeutic applications of silver
nano particles using Flacourtia sepiaria and Rhinacanthus nasutus
T2 - International Journal of Pharma and Bio Sciences
TI - Comparative studies on green synthesis and therapeutic applications of silver
nano particles using Flacourtia sepiaria and Rhinacanthus nasutus
84908011906&partnerID=40&md5=a49856f130fd318461bb7b877984d8ae
VL - 5
ID - 8555
ER -
TY - JOUR
AB - Human immunodeficiency virus (HIV)-1 infection of the central nervous system
occurs in the vast majority of HIV-infected patients. HIV-associated dementia (HAD)
represents the most severe form of HIV-related neuropsychiatric impairment and is
associated with neuropathology involving HIV proteins and activation of
proinflammatory cytokine circuits. Interferon-gamma (IFN-gamma) activates the
JAK/STAT1 pathway, a key regulator of inflammatory and apoptotic signaling, and is
elevated in HIV-1-infected brains progressing to HAD. Recent reports suggest green
tea-derived (-)-epigallocatechin-3-gallate (EGCG) can attenuate neuronal damage
mediated by this pathway in conditions such as brain ischemia. In order to
investigate the therapeutic potential of EGCG to mitigate the neuronal damage
characteristic of HAD, IFN-gamma was evaluated for its ability to enhance well-
known neurotoxic properties of HIV-1 proteins gp120 and Tat in primary neurons and
mice. Indeed, IFN-gamma enhanced the neurotoxicity of gp120 and Tat via increased
JAK/STAT signaling. Additionally, primary neurons pretreated with a JAK1 inhibitor,
or those derived from STAT1-deficient mice, were largely resistant to the IFN-
gamma-enhanced neurotoxicity of gp120 and Tat. Moreover, EGCG treatment of primary
neurons from normal mice reduced IFN-gamma-enhanced neurotoxicity of gp120 and Tat
by inhibiting JAK/STAT1 pathway activation. EGCG was also found to mitigate the
neurotoxic properties of HIV-1 proteins in the presence of IFN-gamma in vivo. Taken
together, these data suggest EGCG attenuates the neurotoxicity of IFN-gamma
augmented neuronal damage from HIV-1 proteins gp120 and Tat both in vitro and in
vivo. Thus EGCG may represent a novel natural copound for the prevention and
treatment of HAD.
AN - rayyan-553782238
AU - Giunta, B.
AU - Obregon, D.
AU - Hou, H.
AU - Zeng, J.
AU - Sun, N.
AU - Nikolic, V.
AU - Ehrhart, J.
AU - Shytle, D.
AU - Fernandez, F.
AU - Tan, J.
DO - 10.1016/j.brainres.2006.09.057
IS - 1
J2 - Brain Res
KW - AIDS Dementia Complex/*drug therapy/enzymology/immunology/pathology
Analysis of Variance
Animals
Apoptosis/drug effects/immunology
Catechin/*analogs & derivatives/therapeutic use
Cells, Cultured
Female
Gene Products, tat/immunology/toxicity
HIV Envelope Protein gp120/immunology/toxicity
HIV-1/*immunology
Interferon-gamma/adverse effects/*metabolism
Janus Kinases/*metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neurons/drug effects/*enzymology/immunology/pathology
Neuroprotective Agents/*therapeutic use
Neurotoxins/immunology/toxicity
STAT1 Transcription Factor/genetics/*metabolism
Signal Transduction/drug effects/physiology
tat Gene Products, Human Immunodeficiency Virus
Dementia
LA - eng
N1 - Neuroimmunology Laboratory, Silver Child Development Center, Institute for
Research in Psychiatry, Department of Psychiatry and Behavioral Medicine,
University of South Florida, 3515 East Fletcher Avenue Tampa, Florida, 33613, USA.;
Neuroimmunology Laboratory, Silver Child Development Center, Institute for Research
in Psychiatry, Department of Psychiatry and Behavioral Medicine, University of
South Florida, 3515 East Fletcher Avenue Tampa, Florida, 33613, USA.;
South Florida, 3515 East Fletcher Avenue Tampa, Florida, 33613, USA.; Center for
Excellence in Aging and Brain Repair, Department of Neurosurgery; University of
South Florida, 3515 East Fletcher Avenue Tampa, Florida, 33613, USA.; Center for
Excellence in Aging and Brain Repair, Department of Neurosurgery; University of
South Florida, 3515 East Fletcher Avenue Tampa, Florida, 33613, USA.
PY - 2006
SN - 0006-8993 (Print)
SP - 216-225
ST - EGCG mitigates neurotoxicity mediated by HIV-1 proteins gp120 and Tat in the
presence of IFN-gamma: role of JAK/STAT1 signaling and implications for HIV-
associated dementia
T2 - Brain research
TI - EGCG mitigates neurotoxicity mediated by HIV-1 proteins gp120 and Tat in the
presence of IFN-gamma: role of JAK/STAT1 signaling and implications for HIV-
associated dementia
VL - 1123
Y2 - 12 y3 - 6
ID - 10148
ER -
TY - JOUR
AB - Silver (Ag) nanoparticles are commonly used in consumer products due to their
antimicrobial properties. Here we studied the impact of Ag nanoparticles on immune
responses by using cell lines of monocyte/macrophage and lung epithelial cell
origin, respectively. Short-term experiments (24 h) showed that Ag nanoparticles
reduced the lipopolysaccharide (LPS)-induced secretion of pro-inflammatory
cytokines in THP-1 cells under serum-free conditions. ICP-MS analysis revealed that
cellular uptake of Ag was higher under these conditions. Long-term exposure (up to
6 weeks) of BEAS-2B cells to Ag nanoparticles also suppressed pro-inflammatory
cytokine production following a brief challenge with LPS. Experiments using
reporter cells revealed that Ag nanoparticles as well as AgNO3 inhibited LPS-
triggered Toll-like receptor (TLR) signaling. Furthermore, RNA-sequencing of BEAS-
2B cells indicated that Ag nanoparticles affected TLR signaling pathways. In
conclusion, Ag nanoparticles reduced the secretion of pro-inflammatory cytokines in
response to LPS, likely as a result of the release of silver ions leading to an
interference with TLR signaling. This could have implications for the use of Ag
nanoparticles as antibacterial agents. Further in vivo studies are warranted to
study this.
AN - rayyan-553780530
AU - Gliga, A. R.
AU - De Loma, J.
AU - Di Bucchianico, S.
AU - Skoglund, S.
AU - Keshavan, S.
AU - Wallinder, I. O.
AU - Karlsson, H. L.
AU - Fadeel, B.
DO - 10.1039/c9na00721k
IS - 2
KW - Humanities
Humanism
Humans
Toll-Like Receptors
Lipopolysaccharides
Cell Line
PY - 2020
SN - 2516-0230
SP - 648-658
ST - Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor
signaling in immune-competent human cell lines
T2 - NANOSCALE ADVANCES
TI - Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor
signaling in immune-competent human cell lines
VL - 2
Y2 - 2 y3 - 1
ID - 8558
ER -
TY - JOUR
AB - Nanotechnology occupies a prominent space in economy and science due to the
beneficial properties of nanomaterials. However, nanoparticles may pose risks to
living organisms due to their adsorption and pro-oxidative properties. The aim of
the current study was to investigate the effects of polymer-coated silver
nanoparticles (AgNPs) and organochlorine pesticides (OCPs), as well as their
combined effects on mouse peritoneal macrophages. Macrophages were isolated and
exposed to three concentrations of AgNPs (groups: N1 = 30, N2 = 300 and N3 = 3000
ng.ml(-1)), two concentrations of OCPs (groups: P1 = 30 and P2 = 300 ng.ml(-1)) and
the six possible combinations of these two contaminants for 24 h. AgNPs had
irregular shape, Feret diameter of 8.7 +/- 7.5 nm and zeta potential of -28.7 +/-
3.9 mV in water and -10.7 +/- 1.04 mV in culture medium. OCP mixtures and the lower
concentrations of AgNPs had no detectable effects on cell parameters, but the
highest AgNPs concentration showed high toxicity (trypan blue and MTT assays)
resulting in morphological changes, increase of nitric oxide levels and phagocytic
index. Foremost, the association of N3 and P2 led to distinct effects from those
observed under single exposure.
AN - rayyan-553780531
AU - Glinski, A.
AU - Liebel, S.
AU - Pelletier, E.
AU - Voigt, C. L.
AU - Randi, M. A. F.
AU - Campos, S. X.
AU - Ribeiro, C. A. O.
AU - Neto, F. F.
DO - 10.3109/15376516.2016.1159770
IS - 4
KW - Mice
Pesticides
Macrophages, Peritoneal
Peritonitis
PY - 2016
SN - 1537-6516 1537-6524
SP - 251-259
ST - Toxicological interactions of silver nanoparticles and organochlorine
pesticides in mouse peritoneal macrophages
T2 - TOXICOLOGY MECHANISMS AND METHODS
TI - Toxicological interactions of silver nanoparticles and organochlorine
pesticides in mouse peritoneal macrophages
VL - 26
ID - 8559
ER -
TY - JOUR
AB - A reconstituted collagen tendon prosthesis was developed and implanted in
rabbit Achilles tendons. The prosthesis was prepared by extruding type-I collagen
into fibers and crosslinking it either with glutaraldehyde or with dehydrothermal
treatment followed by exposure to carbodiimide. A tendon prosthesis was assembled
by coating a longitudinal array of the fibers with uncrosslinked collagen. In one
leg of the rabbit, the Achilles tendon was replaced with the synthetic tendon; in
the contralateral leg of the animal, the tendon was excised, devascularized, and
anastomosed as an autogenous graft. The autogenous tendon grafts were seen to be
infiltrated centrally by fibroblasts and capillaries ten weeks postoperatively and
to have been partially replaced by repair tissue twenty weeks postoperatively.
Three weeks after implantation, all collagen implants were noted to have been
infiltrated with fibrous tissue. At ten weeks, reorganization of collagenous tissue
was observed in and around the prostheses, and the carbodiimide-crosslinked
implants had been resorbed and replaced by normal-appearing neotendon. The implants
that had been treated with glutaraldehyde were resorbed more slowly and were
surrounded by more inflammatory cells, compared with the prostheses that had been
treated with carbodiimide. Neotendon in the glutaraldehyde-treated prostheses
matured more slowly. When the implants were examined at intervals after the
operation, their mechanical properties approached those of fresh tendon. The
initial strength of the carbodiimide-treated implants was lower than that of the
fresh autogenous grafts. Twenty weeks after implantation, the strength and modulus
of the carbodiimide-treated implants approached those of fresh tendon.(ABSTRACT
TRUNCATED AT 250 WORDS)
AN - rayyan-553782332
AU - Goldstein, J. D.
AU - Tria, A. J.
AU - Zawadsky, J. P.
AU - Kato, Y. P.
AU - Christiansen, D.
AU - Silver, F. H.
IS - 8
J2 - J Bone Joint Surg Am
KW - Animals
*Collagen
Cross-Linking Reagents
Glutaral
Hindlimb
*Prostheses and Implants
Rabbits
*Tendons/pathology/surgery
Prostheses and Implants
Tendons
Collagen
Tendinopathy
LA - eng
N1 - University of Medicine and Dentistry of New Jersey, Robert Wood Johnson
Medical School, Piscataway 08854.
PY - 1989
SN - 0021-9355 (Print)
SP - 1183-91
ST - Development of a reconstituted collagen tendon prosthesis. A preliminary
implantation study
T2 - The Journal of bone and joint surgery. American volume
TI - Development of a reconstituted collagen tendon prosthesis. A preliminary
implantation study
VL - 71
Y2 - 9
ID - 10240
ER -
TY - JOUR
AB - Comparative hazard identification of nanomaterials (NMs) can aid in the
prioritisation for further toxicity testing. Here, we assessed the acute lung,
systemic and liver responses in C57BL/6N mice for three NMs to provide a hazard
ranking. A silver (Ag), non-functionalised zinc oxide (ZnO) and a
triethoxycaprylylsilane functionalised ZnO NM suspended in water with 2% mouse
serum were examined 24 hours following a single intratracheal instillation (IT.).
An acute pulmonary inflammation was noted (marked by a polymorphonuclear neutrophil
influx) with cell damage (LDH and total protein) in broncho-alveolar lavage fluid
(BALF) after administration of both non-functionalised and functionalised ZnO. The
latter also induced systemic inflammation measured as an increase in blood
neutrophils and a decrease in blood lymphocytes. Exposure to Ag NM was not
accompanied by pulmonary inflammation or cytotoxicity, or by systemic inflammation.
A decrease in glutathione levels was demonstrated in the liver following exposure
to high doses of all three nanomaterials irrespective of any noticeable
inflammatory or cytotoxic effects in the lung. By applying benchmark dose (BMD)
modeling statistics to compare potencies of the NMs, we rank functionalised ZnO
ranked the highest based on the largest number of affected endpoints, as well as
the strongest responses observed after 24 hours. The non-functionalised ZnO NM gave
an almost similar response, whereas Ag NM did not cause an acute response at
similar doses. © 2015 Gosens et al.
AN - rayyan-553780538
AU - Gosens, I.
AU - Kermanizadeh, A.
AU - Lenz, A. G.
AU - Bokkers, B.
AU - De Jong, W. H.
AU - Krystek, P.
AU - Tran, L.
AU - Stone, V.
AU - Wallin, H.
AU - Stoeger, T.
AU - Cassee, F. R.
DO - 10.1371/journal.pone.0126934
IS - 5
KW - Animals
Injections, Spinal
Instillation, Drug
Lung
Mice
Mice, Inbred C57BL
Nanostructures
Silver
Toxicity Tests
Zinc Oxide
Mus
glutathione
protein
silane derivative
silver
silver nanoparticle
triethoxycaprylylsilane
unclassified drug
water
zinc oxide
nanomaterial
animal cell
animal experiment
animal tissue
Article
C57BL/6N mouse
cell damage
comparative hazard identification
controlled study
cytotoxicity
exposure
female
hazard assessment
inflammation
liver
lung
lung lavage
lymphocyte
mouse
nonhuman
statistical model
toxicity testing
animal
C57BL mouse
chemistry
comparative study
drug effects
drug instillation
intraspinal drug administration
metabolism
pathology
procedures
Zinc
PY - 2015
ST - Comparative hazard identification by a single dose lung exposure of zinc
oxide and silver nanomaterials in mice
T2 - PLoS ONE
TI - Comparative hazard identification by a single dose lung exposure of zinc
oxide and silver nanomaterials in mice
84930678084&doi=10.1371%2fjournal.pone.0126934&partnerID=40&md5=c9844120208678e8a2f
849b3ab41a919
VL - 10
ID - 8566
ER -
TY - JOUR
AB - BACKGROUND: Ovarian cancer has no definitive second line therapeutic options,
and largely recurs in the peritoneal cavity. Locoregional immune therapy using both
interferons and monocytes can be used as a novel approach. Interferons have both
cytostatic and cytotoxic properties, while monocytes stimulated with interferons
have potent cytotoxic properties. Due to the highly immune suppressive properties
of ovarian cancer, ex vivo stimulation of autologous patient monocytes with
interferons and infusion of all three agents intraperitoneally (IP) can provide a
strong pro-inflammatory environment at the site of disease to kill malignant cells.
METHODS: Patient monocytes are isolated through counterflow elutriation and
stimulated ex vivo with interferons and infused IP through a semi-permanent
catheter. We have designed a standard 3 + 3 dose escalation study to explore the
highest tolerated dose of interferons and monocytes infused IP in patients with
chemotherapy resistant ovarian cancer. Secondary outcome measurements of changes in
the peripheral blood immune compartment and plasma cytokines will be studied for
correlations of response. DISCUSSION: We have developed a novel immunotherapy
focused on the innate immune system for the treatment of ovarian cancer. We have
combined the use of autologous monocytes and interferons alpha and gamma for local-
regional administration directly into the peritoneal cavity. This therapy is highly
unique in that it is the first study of its type using only components of the
innate immune system for the locoregional delivery consisting of autologous
monocytes and dual interferons alpha and gamma. Trial Registration
ClinicalTrials.gov Identifier: NCT02948426, registered on October 28, 2016.
https://clinicaltrials.gov/ct2/show/NCT02948426.
AN - rayyan-553782374
AU - Green, D. S.
AU - Nunes, A. T.
AU - David-Ocampo, V.
AU - Ekwede, I. B.
AU - Houston, N. D.
AU - Highfill, S. L.
AU - Khuu, H.
AU - Stroncek, D. F.
AU - Steinberg, S. M.
AU - Zoon, K. C.
AU - Annunziata, C. M.
DO - 10.1186/s12967-018-1569-5
IS - 1
J2 - J Transl Med
KW - Dose-Response Relationship, Drug
Female
Humans
Injections, Intraperitoneal
Interferon alpha-2/*administration & dosage/pharmacology/*therapeutic use
Interferon-alpha/*administration & dosage/pharmacology/*therapeutic use
Interferon-gamma/*administration & dosage/pharmacology/*therapeutic use
Monocytes/drug effects/*metabolism
Neoplasm Recurrence, Local/*drug therapy
Ovarian Neoplasms/*drug therapy
Polyethylene Glycols/*administration & dosage/pharmacology/*therapeutic use
Recombinant Proteins/administration & dosage/pharmacology/therapeutic use
Treatment Outcome
Monocytes
Interferon-gamma
Ovarian Neoplasms
Interferons
Recurrence
LA - eng
N1 - Women's Malignancies Branch, Center for Cancer Research, National Cancer
Institute, National Institutes of Health, 10 Center Drive RM 3B43C, Bethesda, MD,
20892, USA.; Women's Malignancies Branch, Center for Cancer Research, National
Cancer Institute, National Institutes of Health, 10 Center Drive RM 3B43C,
Bethesda, MD, 20892, USA.; Cell Processing Section, Department of Transfusion
Medicine, Clinical Center, National Institutes of Health, Bethesda, USA.; Office of
Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, FDA,
Silver Spring, MD, USA.; Women's Malignancies Branch, Center for Cancer Research,
National Cancer Institute, National Institutes of Health, 10 Center Drive RM 3B43C,
Bethesda, MD, 20892, USA.; Women's Malignancies Branch, Center for Cancer Research,
National Cancer Institute, National Institutes of Health, 10 Center Drive RM 3B43C,
Bethesda, MD, 20892, USA.; Cell Processing Section, Department of Transfusion
Medicine, Clinical Center, National Institutes of Health, Bethesda, USA.; Cell
Processing Section, Department of Transfusion Medicine, Clinical Center, National
Institutes of Health, Bethesda, USA.; Office of Tissues and Advanced Therapies,
Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.; Cell
Processing Section, Department of Transfusion Medicine, Clinical Center, National
Institutes of Health, Bethesda, USA.; Biostatistics and Data Management Section,
National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.;
Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious
Diseases, National Institutes of Health Bethesda, Bethesda, MD, USA.; Women's
Malignancies Branch, Center for Cancer Research, National Cancer Institute,
National Institutes of Health, 10 Center Drive RM 3B43C, Bethesda, MD, 20892, USA.
annunzic@mail.nih.gov.
PY - 2018
SP - 196
ST - A Phase 1 trial of autologous monocytes stimulated ex vivo with Sylatron(®)
(Peginterferon alfa-2b) and Actimmune(®) (Interferon gamma-1b) for intra-peritoneal
administration in recurrent ovarian cancer
T2 - Journal of translational medicine
TI - A Phase 1 trial of autologous monocytes stimulated ex vivo with Sylatron(®)
(Peginterferon alfa-2b) and Actimmune(®) (Interferon gamma-1b) for intra-peritoneal
administration in recurrent ovarian cancer
VL - 16
Y2 - 7 y3 - 16
ID - 10281
ER -
TY - JOUR
AB - Highly refined mineral hydrocarbons (MHCs) such as low melting point paraffin
wax (LMPW) and low viscosity white oils can cause inflammatory changes in the liver
and mesenteric lymph nodes (MLNs) of the Fischer-344 (F-344) rat. In contrast, only
minimal MLN changes are seen in the Sprague-Dawley (S-D) rat with no changes in the
liver. In this study, the response of female F-344 and S-D rats was compared after
90 days dietary treatment with 0%. 0.2% or 2% LMPW. Effects in the F-344 rats were
significantly greater than in the S-D rats: increased liver and splenic weights and
inflammatory changes (hepatic microgranulomas) in these tissues were observed only
in the F-344 rats. Microgranulomas in the MLNs were observed in both strains but
the effects were substantially greater in the F-344 rats. Cellular markers of
inflammation were examined in a subset of rats from each group using
immunohistochemical staining. An increase in staining for CD3 (T-cells), CD8a
(suppresser/cytotoxic T-cells) and CD4 (helper T-cells) correlated with an increase
in lymphoid cells in the livers of treated F-344 rats. The majority of macrophages
in the hepatic microgranulomas of treated F-344 rats were negative for the ED2
marker, indicating a likely origin from non-resident macrophages. Electron
microscopy showed Kupffer cell hypertrophy and hyperplasia in treated F-344 rats.
However, lysozyme staining (indicating activation of epithelioid macrophages)
decreased with increasing granuloma size. Non-ED2 expressing cells may have been
recruited but not sufficiently activated to be lysozyme positive. Inflammatory
changes in the cardiac mitral valve noted in previous studies of LMPW were also
seen in the F-344 rats in this study but not in the S-D rats. Chemical analysis
showed that MHC accumulated in livers from treated F-344 but not S-D rats and the
concentration was more than 2-fold greater in MLNs from the F-344 than from the S-D
rats. The F-344 appears to be more immunologically sensitive to a number of agents
than other rat strains and the results of this study suggest that this may
contribute, along with pharmacokinetic differences, to the inflammatory response of
F-344 rats to dietary MHCs. (C) 2009 Elsevier Ltd. All rights reserved.
AN - rayyan-553780540
AU - Griffis, L. C.
AU - Twerdok, L. E.
AU - Francke-Carroll, S.
AU - Biles, R. W.
AU - Schroeder, R. E.
AU - Bolte, H.
AU - Faust, H.
AU - Hall, W. C.
AU - Rojko, J.
DO - 10.1016/j.fct.2009.10.024
IS - 1
KW - Rats
PY - 2010
SN - 0278-6915
SP - 363-372
ST - Comparative 90-day dietary study of paraffin wax in Fischer-344 and Sprague-
Dawley rats
T2 - FOOD AND CHEMICAL TOXICOLOGY
TI - Comparative 90-day dietary study of paraffin wax in Fischer-344 and Sprague-
Dawley rats
VL - 48
Y2 - 1
ID - 8568
ER -
TY - JOUR
AB - The inhalation toxicology of ricin (supplied by Sigma) from the seed variety
"Hale Queen" and abrin was examined following head-only exposure of rats to a range
of concentrations of each toxin generated as an aerosol from solution using a
constant-output nebulizer. The inhalation toxicity of an in-house preparation of
ricin from a different seed type, Ricinus communis var. zanzibariensis (R.
zanzibariensis), was also assessed for comparison. The approximate LCt50 values
determined were very similar for the Sigma ricin and abrin (4.54-5.96 and 4.54 mg
min m-3, respectively). However, the LCt50 of ricin toxin prepared in-house from
seeds of the R. zanzibariensis variety was assessed to be 12.7 mg min m-3. Ricin
prepared from this seed variety was therefore less toxic than Sigma ricin by a
factor of almost threefold. Given that both ricin preparations were pure by silver-
stained, sodium dodecyl sulfate polyacrylamide electrophoresis gels, the data must
reflect differences in specific toxicity between seed varieties. The histopathology
was studied in a separate group of experimental animals exposed to approximate
LCt30 levels of each in-house toxin preparation and was found to be entirely
restricted to the lung. The overall pattern and time course of damage observed were
similar for ricin and abrin and were characterized by rapidly progressive and
overwhelming pulmonary edema accompanied by acute destructive alveolitis and
necrosis/apoptosis of the lower respiratory tract epithelium; severe intraalveolar
edema and resulting hypoxia accounted for the majority of deaths in the decedent
population. The resolution phase of the pulmonary damage in those animals destined
to survive was heralded by a gradual disappearance of edema fluid accompanied by
generalized, focally florid, hyperplasia of type II pneumocytes and striking
transitory consolidation of the lung parenchyma by chronic inflammatory cells.
Despite many similarities in histopathology between abrin and ricin there were some
differences. Although by systemic administration abrin is several times more toxic
than ricin, when delivered by inhalation there was no significant difference in
potency between abrin and the commercial preparation of ricin (Sigma). © 1995
Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
AN - rayyan-553780541
AU - Griffiths, G. D.
AU - Rice, P.
AU - Allenby, A. C.
AU - Bailey, S. C.
AU - Upshall, D. G.
DO - 10.3109/08958379509029098
IS - 2
PY - 1995
SP - 269-288
ST - Inhalation toxicology and histopathology of ricin and abrin toxins
T2 - Inhalation Toxicology
TI - Inhalation toxicology and histopathology of ricin and abrin toxins
0000876903&doi=10.3109%2f08958379509029098&partnerID=40&md5=7ae661ae7db2c2dd437c5ef
23ac2cc67
VL - 7
ID - 8569
ER -
TY - JOUR
AB - Polyethylene terephthalate (PET) is a major pollutant polymer, due to its
wide use in food packaging and fiber production industries worldwide. Currently,
there is great interest for recycling the huge amount of PET-based materials,
derived especially from the food and textile industries. In this study, we applied
the electrospinning technique to obtain nanostructured fibrillary membranes based
on PET materials. Subsequently, the recycled PET networks were decorated with
silver nanoparticles through the chemical reduction method for antimicrobial
applications. After the characterization of the materials in terms of
crystallinity, chemical bonding, and morphology, the effect against Gram-positive
and Gram-negative bacteria, as well as fungal strains, was investigated.
Furthermore, in vitro and in vivo biocompatibility tests were performed in order to
open up potential biomedical applications, such as wound dressings or implant
coatings. Silver-decorated fibers showed lower cytotoxicity and inflammatory
effects and increased antibiofilm activity, thus highlighting the potential of
these systems for antimicrobial purposes. © 2019 by the authors. Licensee �MDPI,
Basel, Switzerland.
AN - rayyan-553780542
AU - Stoica, A. E.
AU - Dima-Bălcescu, M. Ș
AU - Chircov, C.
AU - Gharbia, S.
AU - Baltă, C.
AU - Roșu, M.
AU - Herman, H.
AU - Holban, A. M.
AU - Ficai, A.
AU - Vasile, B. S.
AU - Andronescu, E.
AU - Chifiriuc, M. C.
AU - Hermenean, A.
DO - 10.3390/jcm8071039
IS - 7
Biocompatibility
Electrospinning
Nanofibers
PET
Polyethylene terephthalate
C reactive protein
nanofiber
polybutylene terephthalate
silver nanoparticle
amniotic fluid stem cell
animal experiment
animal model
animal tissue
anti-infective therapy
antibiofilm activity
antifungal activity
Article
bacterial strain
biochemical analysis
biocompatibility
biofilm
biological activity
blood sampling
chemical binding
chemical reaction
controlled study
cytotoxicity
electrospinning
fungal strain
histopathology
human
human cell
mouse
MTT assay
nonhuman
particle size
phagocytosis
plankton
X ray diffraction
Polyethylenes
Polyethylene
PY - 2019
ST - Electrospun polyethylene terephthalate nanofibers loaded with silver
nanoparticles: Novel approach in anti-infective therapy
T2 - Journal of Clinical Medicine
TI - Electrospun polyethylene terephthalate nanofibers loaded with silver
nanoparticles: Novel approach in anti-infective therapy
85076947131&doi=10.3390%2fjcm8071039&partnerID=40&md5=062acb5060622c269804d1f587ef8
be3
VL - 8
ID - 8570
ER -
TY - JOUR
AB - Responsive, theranostic nanosystems, capable of both signaling and treating
wound infections, is a sophisticated approach to reduce the most common and
potentially traumatizing side effects of burn wound treatment: slowed wound healing
due to prophylactic anti-infective drug exposure as well as frequent painful
dressing changes. Antimicrobials as well as dye molecules have been incorporated
into biodegradable nanosystems that release their content only in the presence of
pathogens. Following nanocarrier degradation by bacterial enzymes, any infection
will thus emit a visible signal and be effectively treated at its source. In this
study, we investigated the effect of fluorescent-labeled hyaluronan nanocapsules
containing polyhexanide biguanide and poly-L-lactic acid nanoparticles loaded with
octenidine on primary human dermal microvascular endothelial cells, which play a
major role in cutaneous wound healing. Microscopic and flow cytometric analysis
indicated a time-dependent uptake of both the nanocapsules and the nanoparticles.
However, enzyme immunoassays showed no significant influence on the expression of
pro-inflammatory cell adhesion molecules and cytokines by the endothelial cells.
Under angiogenic-stimulating conditions, the potential to form capillary-like
structures in coculture with dermal fibroblasts was not inhibited. Furthermore,
cytotoxicity studies (the MTS and crystal violet assay) after short- and long-term
exposure to the materials demonstrated that both systems exhibited less toxicity
than solutions of the antiseptic agents alone in comparable concentrations. The
results indicate that responsive antimicrobial nanocomposites could be used as an
advanced drug delivery system and a promising addition to current best practice
wound infection prophylaxis with few side effects.
AN - rayyan-553780543
AU - Grutzner, V.
AU - Unger, R. E.
AU - Baier, G.
AU - Choritz, L.
AU - Freese, C.
AU - Bose, T.
AU - Landfester, K.
AU - Kirkpatrick, C. J.
DO - 10.2147/IJN.S81263
KW - Burns
PY - 2015
SN - 1178-2013
SP - 4111-4124
ST - Enzyme-responsive nanocomposites for wound infection prophylaxis in burn
management: in vitro evaluation of their compatibility with healing processes
TI - Enzyme-responsive nanocomposites for wound infection prophylaxis in burn
management: in vitro evaluation of their compatibility with healing processes
VL - 10
ID - 8571
ER -
TY - JOUR
AB - The development of nanotechnologies is leading to greater abundance of
engineered nanoparticles (EN) in the environment, including in the atmospheric air.
To date, it has been shown that the most prevalent EN found in the air are silver
(Ag), titanium dioxide (TiO2), titanium (Ti), and silicon dioxide (SiO2). As the
intestinal tract is increasingly recognized as a target for adverse effects induced
by inhalation of air particles, the aim of this study was to assess the impact of
these 4 atmospheric EN on intestinal inflammation and microbiota. We assessed the
combined toxicity effects of Ag, Ti, TiO2, and SiO2 following a 28-day inhalation
protocol in male and female mice. In distal and proximal colon, and in jejunum, EN
mixture inhalation did not induce overt histological damage, but led to a
significant modulation of inflammatory cytokine transcript abundance, including
downregulation of Tnfα, Ifnγ, Il1β, Il17a, Il22, IL10, and Cxcl1 mRNA levels in
male jejunum. A dysbiosis was observed in cecal microbiota of male and female mice
exposed to the EN mixture, characterized by sex-dependent modulations of specific
bacterial taxa, as well as sex-independent decreased abundance of the
Eggerthellaceae family. Under dextran sodium sulfate-induced inflammatory
conditions, exposure to the EN mixture increased the development of colitis in both
male and female mice. Moreover, the direct dose-response effects of individual and
mixed EN on gut organoids was studied and Ag, TiO2, Ti, SiO2, and EN mixture were
found to generate specific inflammatory responses in the intestinal epithelium.
These results indicate that the 4 most prevalent atmospheric EN could have the
ability to disturb intestinal homeostasis through direct modulation of cytokine
expression in gut epithelium, and by altering the inflammatory response and
microbiota composition following inhalation. © 2022 The Authors
AN - rayyan-553780547
AU - Guilloteau, E.
AU - Djouina, M.
AU - Caboche, S.
AU - Waxin, C.
AU - Deboudt, K.
AU - Beury, D.
AU - Hot, D.
AU - Pichavant, M.
AU - Dubuquoy, L.
AU - Launay, D.
AU - Vignal, C.
AU - Choël, M.
AU - Body-Malapel, M.
DO - 10.1016/j.ecoenv.2022.113442
KW - Colitis
Digestive tract
Engineered nanoparticles
Inflammation
Inhalation
Intestine
Microbiome
Mixture
Organoid
Animals
Cytokines
Female
Male
Mice
Microbiota
Nanoparticles
Silicon Dioxide
Titanium
cytokine
dextran sulfate
silica nanoparticle
silver nanoparticle
titanium
nanoparticle
silicon dioxide
titanium dioxide
digestive system
nanotechnology
rodent
silicon
silver
animal experiment
animal tissue
Article
ascending colon
atmospheric particulate matter
colitis
controlled study
Cxcl1 gene
descending colon
dose response
down regulation
dysbiosis
Eggerthellaceae
enteritis
environmental impact assessment
female
gastrointestinal toxicity
gene
genetic transcription
homeostasis
Ifngamma gene
IL10 gene
Il17a gene
Il1beta gene
Il22 gene
inhalation
intestine epithelium
intestine flora
jejunum
male
modulation
mouse
nanoengineering
nonhuman
organoid
population abundance
species composition
taxonomy
tissue injury
Tnfa gene
animal
genetics
metabolism
microflora
PY - 2022
ST - Exposure to atmospheric Ag, TiO2, Ti and SiO2 engineered nanoparticles
modulates gut inflammatory response and microbiota in mice
T2 - Ecotoxicology and Environmental Safety
TI - Exposure to atmospheric Ag, TiO2, Ti and SiO2 engineered nanoparticles
modulates gut inflammatory response and microbiota in mice
85127225863&doi=10.1016%2fj.ecoenv.2022.113442&partnerID=40&md5=b307503dd2cb07a8f17
261a6f46c5930
VL - 236
ID - 8575
ER -
TY - JOUR
AB - Pinus roxburghii Sarg., commonly known as “chir pine,” belonging to the
family Pinaceae, is found in the Himalayan region of Tibet, Bhutan, Nepal,
Pakistan, and North Indian provinces. Due to its medicinal properties, it has been
used by folklore for treating several ailments. Several medicinal characteristics
like wound healing activity, cognitive enhancement, anti-inflammatory, anti-
diabetic, anti-dyslipidemic, anticonvulsant, hepatoprotection, and anticancer
properties have been proved scientifically. The medicinal properties of P.
roxburghii can be attributed to the presence of various secondary metabolites. This
review focuses on medicinal properties and secondary metabolites present in P.
roxburghii found in different regions like Egypt, India, Nepal, and Pakistan as
analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). GC-MS analysis for
secondary metabolites of P. roxburghii species from Egypt, India, and Pakistan
revealed dominated compounds like α-pinene, 3-carene, caryophyllene, whereas P.
roxburghii samples from Nepal showed major compounds like terpinen- 4-ol and
eugenol. Similarly, samples from Egypt were also found to be enriched with α-
thujene and d-longifolene. On the other hand, α-terpinene and α-phellandrene were
identified as major compounds in samples from India. These variations in the
phytocomponents of P. roxburghii can be attributed to the geographical conditions
of these places. © 2021, Indian journals. All rights reserved.
AN - rayyan-553780548
AU - Gulilat, H.
AU - Saini, A. K.
AU - Saini, R. V.
DO - 10.5958/0975-6892.2021.00046.0
IS - 3
KW - GC-MS
Geographical location
Natural product
P. roxburghii
Phytoconstituents
1,1 diphenyl 2 picrylhydrazyl
3 carene
acetic acid ethyl ester
acetone
albumin
alcohol
aldehyde reductase
alkaloid
alpha phellandrene
alpha thujene
anthracene
baculoviral IAP repeat containing protein 5
bilirubin
butanol
camphene
carbohydrate
cardiac glycoside
caryophyllene
caspase 3
chloroform
cholesterol
coumarin
dipeptidyl peptidase IV
essential oil
eugenol
flavonoid
FLICE inhibitory protein
gelatinase B
glutathione
glycerol
glycoside
hexane
high density lipoprotein cholesterol
insulin receptor
interleukin 6
lignan
limonene
malonaldehyde
methanol
myeloperoxidase
nitric oxide
ocimene
palmitic acid
phenol
phosphorothioic acid o,o,o trimethyl ester
phytochemical
pinene
pinoresinol
plant extract
poly(adenosine diphosphate ribose)
protein bcl 2
protein bcl x
protein bcl xl
protein tyrosine phosphatase 1B
resin
RNA directed DNA polymerase
saponin
sesquiterpene
silver nanoparticle
stilbene
sugar
tannin
terpenoid
terpinen 4 ol
terpinene
triacylglycerol
turpentine oil
water
xanthone derivative
A-549 cell line
Agrobacterium tumefaciens
alkaline phosphatase blood level
antiaging activity
anticonvulsant activity
antimalarial activity
antiviral activity
apoptosis
Article
bilirubin blood level
bioassay
Candida albicans
cholesterol synthesis
cognition
cytotoxicity
distillation
electric shock
Enterobacter aerogenes
epithelization
Escherichia coli
folklore
frontal cortex
HCT 116 cell line
hippocampus
KBM-5 cell line
liquid chromatography
liver histology
liver injury
liver protection
lymphocytic infiltration
MCF-7 cell line
mental disease
necrosis
neuroprotection
nonhuman
oxidative stress
pentylenetetrazole-induced seizure
pine
SCC-4 cell line
systematic review
T-47D cell line
U266B1 cell line
wound healing
Multiple Sclerosis
PY - 2021
SP - 396-408
ST - The comparative analysis of pinus roxburghii phytoconstituents found at
various asian locations as determined by gc-ms and its medicinal properties
T2 - Medicinal Plants
TI - The comparative analysis of pinus roxburghii phytoconstituents found at
various asian locations as determined by gc-ms and its medicinal properties
85118489423&doi=10.5958%2f0975-
6892.2021.00046.0&partnerID=40&md5=08217113edbdc33b87bc3aea715fca6d
VL - 13
ID - 8576
ER -
TY - JOUR
AB - Experimental modeling to identify specific inhalation hazards for
nanomaterials has in the main focused on in vivo approaches. However, these models
suffer from uncertainties surrounding species-specific differences and cellular
targets for biologic response. In terms of pulmonary exposure, approaches which
combine 'inhalation-like' nano-particulate aerosol deposition with relevant human
cell and tissue air-liquid interface cultures are considered an important
complement to in vivo work. In this study, we utilized such a model system to build
on previous results from in vivo exposures, which highlighted the small airway
epithelium as a target for silver nanoparticle (AgNP) deposition. RNA-SEQ was used
to characterize alterations in mRNA and miRNA within the lung. Organotypic-
reconstituted 3D human primary small airway epithelial cell cultures (SmallAir)
were exposed to the same spark-generated AgNP and at the same dose used in vivo, in
an aerosol-exposure air-liquid interface (AE-ALI) system. Adverse effects were
characterized using lactate, LDH release and alterations in mRNA and miRNA. Modest
toxicological effects were paralleled by significant regulation in gene expression,
reflective mainly of specific inflammatory events. Importantly, there was a level
of concordance between gene expression changes observed in vitro and in vivo. We
also observed a significant correlation between AgNP and mass equivalent silver ion
(Ag+) induced transcriptional changes in SmallAir cultures. In addition to key
mechanistic information relevant for our understanding of the potential health
risks associated with AgNP inhalation exposure, this work further highlights the
small airway epithelium as an important target for adverse effects.
AN - rayyan-553780550
AU - Guo, C.
AU - Buckley, A.
AU - Marczylo, T.
AU - Seiffert, J.
AU - Romer, I.
AU - Warren, J.
AU - Hodgson, A.
AU - Chung, K. F.
AU - Gant, T. W.
AU - Smith, R.
AU - Leonard, M. O.
DO - 10.1080/17435390.2018.1465140
IS - 6
KW - Epithelium
PY - 2018
SN - 1743-5390 1743-5404
SP - 539-553
ST - The small airway epithelium as a target for the adverse pulmonary effects of
silver nanoparticle inhalation
T2 - NANOTOXICOLOGY
TI - The small airway epithelium as a target for the adverse pulmonary effects of
VL - 12
ID - 8578
ER -
TY - JOUR
AB - Experimental modeling to identify specific inhalation hazards for
nanomaterials has in the main focused on in vivo approaches. However, these models
suffer from uncertainties surrounding species-specific differences and cellular
targets for biologic response. In terms of pulmonary exposure, approaches which
combine 'inhalation-like' nanoparticulate aerosol deposition with relevant human
cell and tissue air-liquid interface cultures are considered an important
complement to in vivo work. In this study, we utilized such a model system to build
on previous results from in vivo exposures, which highlighted the small airway
epithelium as a target for silver nanoparticle (AgNP) deposition. RNA-SEQ was used
to characterize alterations in mRNA and miRNA within the lung. Organotypic-
reconstituted 3D human primary small airway epithelial cell cultures (SmallAir)
were exposed to the same spark-generated AgNP and at the same dose used in vivo, in
an aerosol-exposure air-liquid interface (AE-ALI) system. Adverse effects were
characterized using lactate, LDH release and alterations in mRNA and miRNA. Modest
toxicological effects were paralleled by significant regulation in gene expression,
reflective mainly of specific inflammatory events. Importantly, there was a level
of concordance between gene expression changes observed in vitro and in vivo. We
also observed a significant correlation between AgNP and mass equivalent silver ion
(Ag(+)) induced transcriptional changes in SmallAir cultures. In addition to key
mechanistic information relevant for our understanding of the potential health
risks associated with AgNP inhalation exposure, this work further highlights the
small airway epithelium as an important target for adverse effects.
AN - rayyan-553782162
AU - Guo, C.
AU - Buckley, A.
AU - Marczylo, T.
AU - Seiffert, J.
AU - Römer, I.
AU - Warren, J.
AU - Hodgson, A.
AU - Chung, K. F.
AU - Gant, T. W.
AU - Smith, R.
AU - Leonard, M. O.
DO - 10.1080/17435390.2018.1465140
IS - 6
J2 - Nanotoxicology
KW - Aerosols
Animals
Cells, Cultured
Epithelium/drug effects/metabolism
Humans
Inhalation Exposure
Lung/*drug effects/metabolism
Male
Rats
Silver/*toxicity
Epithelium
LA - eng
N1 - a Centre for Radiation, Chemical and Environmental Hazards , Public Health
England , Oxfordshire , UK.; b The National Institute for Health Research Health
Protection Research Unit (NIHR HPRU) in Health Impact of Environmental Hazards at
King's College London in partnership with Public Health England (PHE) in
collaboration with Imperial College London , London , UK.; a Centre for Radiation,
Chemical and Environmental Hazards , Public Health England , Oxfordshire , UK.; b
The National Institute for Health Research Health Protection Research Unit (NIHR
HPRU) in Health Impact of Environmental Hazards at King's College London in
partnership with Public Health England (PHE) in collaboration with Imperial College
London , London , UK.; a Centre for Radiation, Chemical and Environmental Hazards ,
Public Health England , Oxfordshire , UK.; b The National Institute for Health
Research Health Protection Research Unit (NIHR HPRU) in Health Impact of
Environmental Hazards at King's College London in partnership with Public Health
England (PHE) in collaboration with Imperial College London , London , UK.; c
Airways Disease, National Heart & Lung Institute, Imperial College , London , UK.;
a Centre for Radiation, Chemical and Environmental Hazards , Public Health
England , Oxfordshire , UK.; b The National Institute for Health Research Health
Protection Research Unit (NIHR HPRU) in Health Impact of Environmental Hazards at
King's College London in partnership with Public Health England (PHE) in
collaboration with Imperial College London , London , UK.; a Centre for Radiation,
Chemical and Environmental Hazards , Public Health England , Oxfordshire , UK.; a
Centre for Radiation, Chemical and Environmental Hazards , Public Health England ,
Oxfordshire , UK.; c Airways Disease, National Heart & Lung Institute, Imperial
College , London , UK.; a Centre for Radiation, Chemical and Environmental
Hazards , Public Health England , Oxfordshire , UK.; b The National Institute for
Health Research Health Protection Research Unit (NIHR HPRU) in Health Impact of
Environmental Hazards at King's College London in partnership with Public Health
England (PHE) in collaboration with Imperial College London , London , UK.; a
Centre for Radiation, Chemical and Environmental Hazards , Public Health England ,
Oxfordshire , UK.; b The National Institute for Health Research Health Protection
Research Unit (NIHR HPRU) in Health Impact of Environmental Hazards at King's
College London in partnership with Public Health England (PHE) in collaboration
with Imperial College London , London , UK.; a Centre for Radiation, Chemical and
Environmental Hazards , Public Health England , Oxfordshire , UK.; b The National
Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Health
Impact of Environmental Hazards at King's College London in partnership with Public
Health England (PHE) in collaboration with Imperial College London , London , UK.
PY - 2018
SP - 539-553
ST - The small airway epithelium as a target for the adverse pulmonary effects of
T2 - Nanotoxicology
TI - The small airway epithelium as a target for the adverse pulmonary effects of
VL - 12
Y2 - 8
ID - 10073
ER -
TY - JOUR
AB - The thermoplasmonic properties of platinum nanoparticles (PtNPs) render them
desirable for use in diagnosis, detection, therapy, and surgery. However, their
toxicological effects and impact at the molecular level remain obscure.
Nanotoxicology is mainly focused on the interactions of nanostructures with
biological systems, particularly with an emphasis on elucidating the relationship
between the physical and chemical properties such as size and shape. Therefore, we
hypothesized whether these unique anisotropic nanoparticles could induce
cytotoxicity similar to that of spherical nanoparticles and the mechanism involved.
Thus, we synthesized unique and distinct anisotropic PtNPs using lycopene as a
biological template and investigated their biological activities in model human
acute monocytic leukemia (THP-1) macrophages. Exposure to PtNPs for 24 h dose-
dependently decreased cell viability and proliferation. Levels of the cytotoxic
markers lactate dehydrogenase and intracellular protease significantly and dose-
dependently increased with PtNP concentration. Furthermore, cells incubated with
PtNPs dose-dependently produced oxidative stress markers including reactive oxygen
species (ROS), malondialdehyde, nitric oxide, and carbonylated protein. An
imbalance in pro-oxidants and antioxidants was confirmed by significant decreases
in reduced glutathione, thioredoxin, superoxide dismutase, and catalase levels
against oxidative stress. The cell death mechanism was confirmed by mitochondrial
dysfunction and decreased ATP levels, mitochondrial copy numbers, and PGC-1 alpha
expression. To further substantiate the mechanism of cell death mediated by
endoplasmic reticulum stress (ERS), we determined the expression of the inositol-
requiring enzyme (IRE1), (PKR-like ER kinase) PERK, activating transcription factor
6 (ATF6), and activating transcription factor 4 ATF4, the apoptotic markers p53,
Bax, and caspase 3, and the anti-apoptotic marker Bcl-2. PtNPs could activate ERS
and apoptosis mediated by mitochondria. A proinflammatory response to PtNPs was
confirmed by significant upregulation of interleukin-1-beta (IL-1 beta), interferon
gamma (IFN gamma), tumor necrosis factor alpha (TNF alpha), and interleukin (IL-6).
Transcriptomic and molecular pathway analyses of THP-1 cells incubated with the
half maximal inhibitory concentration (IC50) of PtNPs revealed the altered
expression of genes involved in protein misfolding, mitochondrial function, protein
synthesis, inflammatory responses, and transcription regulation. We applied
transcriptomic analyses to investigate anisotropic PtNP-induced toxicity for
further mechanistic studies. Isotropic nanoparticles are specifically used to
inhibit non-specific cellular uptake, leading to enhanced in vivo bio-distribution
and increased targeting capabilities due to the higher radius of curvature. These
characteristics of anisotropic nanoparticles could enable the technology as an
attractive platform for nanomedicine in biomedical applications.
AN - rayyan-553780553
AU - Gurunathan, S.
AU - Jeyaraj, M.
AU - La, H.
AU - Yoo, H.
AU - Choi, Y.
AU - Do, J. T.
AU - Park, C.
AU - Kim, J. H.
AU - Hong, K.
DO - 10.3390/ijms21020440
IS - 2
KW - Humanities
Humanism
Humans
Transcriptome
PY - 2020
SN - 1422-0067
ST - Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis,
Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute
Monocytic Leukemia Cells
TI - Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis,
Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute
Monocytic Leukemia Cells
VL - 21
Y2 - 1 y3 - 2
ID - 8581
ER -
TY - JOUR
AB - Background Gold nanoparticles (AuNPs) have potential for a wide range of
applications as therapeutic and diagnostic agents. Since they have a high
probability of interacting with human immune cells, cytotoxicity studies must be
conducted. The investigation of AuNP/immune cell interaction has mainly focused on
macrophages and dendritic cells, along with some other cell lineages. Scarce
information is available regarding the effect of AuNPs on mast cells, which are
abundant in the skin, mucosa, and perivascular space. Objective To examine the
uptake of AuNPs by HMC-1 human mast cells and the resulting effect on cell
viability, pro-inflammatory mediators production, and proliferation. Results With
AuNPs treatment, the viability of HMC-1 cells decreased slightly (never less than
95%) during the first 4 h, but no changes were detected in the proliferation rate
at any time. Increasing concentrations of AuNPs produced greater cell granularity
(uptake). CLSM images exhibited AuNPs clusters in the cell cytoplasm. TNF-alpha and
ROS production was not stimulated by AuNPs treatment at any concentration/time.
Conclusion Internalization of AuNPs into HMC-1 cells was demonstrated in an in
vitro model, without showing cytotoxic effects or induction of pro-inflammatory
mediators at any concentration tested.
AN - rayyan-553780555
AU - Gutierrez-Calleja, R. A.
AU - Rodriguez-Cortes, O.
AU - Flores-Mejia, R.
AU - Munoz-Diosdado, A.
DO - 10.1007/s13273-021-00152-7
IS - 4
KW - Humanities
Humanism
Humans
Nanoparticles
PY - 2021
SN - 1738-642X 2092-8467
SP - 439-452
ST - Gold nanoparticles: uptake in human mast cells and effect on cell viability,
inflammatory mediators, and proliferation
T2 - MOLECULAR & CELLULAR TOXICOLOGY
TI - Gold nanoparticles: uptake in human mast cells and effect on cell viability,
inflammatory mediators, and proliferation
VL - 17
Y2 - 10
ID - 8583
ER -
TY - JOUR
AB - In recent years, there have been remarkable efforts to examine and understand
the adverse effects of nanoparticles (NPs) on the environment and human health, not
only qualitatively but also quantitatively. Mass cytometry has been developed for
high-dimensional single-cell analyses and used to quantify the cellular association
of inorganic NPs. Here, we have applied this novel technique to investigate the
heterogeneity in cellular association and cytotoxicity of polyvinylpyrrolidone-
coated silver nanoparticles with diameters of 10 nm and 20 nm in primary human
immune cells. Our results revealed the cell-type-dependent heterogeneity in which
AgNPs showed higher affinity to phagocytic cells like monocytes and dendritic cells
than to other immune cell types. Upon exposure to AgNPs, these cells exhibited
complex pro-inflammatory and pro-apoptotic responses, such as IκBα degradation,
STAT1 phosphorylation and caspase-7 activation. Quantitative analyses of the
single-cell dose-response relationship between the cellular AgNP association and
signalling activities further revealed heterogeneity even within a monocyte
population. The majority of cells belonged to the 'low affinity' subset, which
showed a positive AgNP dose-cisplatin uptake (i.e. viability loss) correlation, as
opposed to the remaining cells which belonged to the 'high affinity' subset and had
an insignificant relationship between the cell-associated AgNP amount and cisplatin
uptake/viability loss level. These subsets were distinctly responsive to the
cellular AgNP content, as they showed different levels of signalling proteins such
as IκBα, STAT1 and caspase-7. Our study can be helpful for further understanding
the heterogeneous nature of cell-NP interactions and development of dose-response
models at the single-cell level for various NPs, which will provide key information
for the safe use of nanomaterials in biomedical applications. This journal is © The
Royal Society of Chemistry.
AN - rayyan-553780556
AU - Ha, M. K.
AU - Choi, J. S.
AU - Kwon, S. J.
AU - Song, J.
AU - Lee, Y.
AU - Kim, Y. E.
AU - Yoon, T. H.
DO - 10.1039/c9en01104h
IS - 4
KW - Cells
Cytology
Metal nanoparticles
Apoptotic response
Cellular association
Dose response relationships
Dose-response models
Poly vinyl pyrrolidone
Signalling proteins
Singlecell analysis
apoptosis
cell
nanoparticle
silver
Cell signaling
Humanities
Humanism
Humans
PY - 2020
SP - 1102-1114
ST - Mass cytometric study on the heterogeneity in cellular association and
cytotoxicity of silver nanoparticles in primary human immune cells
T2 - Environmental Science: Nano
TI - Mass cytometric study on the heterogeneity in cellular association and
cytotoxicity of silver nanoparticles in primary human immune cells
85081742245&doi=10.1039%2fc9en01104h&partnerID=40&md5=10366e3c8dc40f34df191f461030a
396
VL - 7
ID - 8584
ER -
TY - JOUR
AB - Silver is commonly used as an antibacterial agent, e. g., in various medical
applications, and the availability of silver nanoparticles (AgNP) has fueled this
development. Their antibacterial properties are well defined, whereas there are
concerns regarding unknown and potentially harmful effects of AgNPs on immune cells
and an ongoing immune reaction. Aim of the present study is a comparison of the
effects of AgNPs and ionic silver (Ag+) on cells of the innate immune system, in
particular on neutrophil granulocytes and macrophages. The AgNPs were synthesized
within hydroxylated polyester dendrimer templates via an in situ approach,
generating five kinds of AgNPs with mean diameters from 2.0 to 34.7 nm. 4 No impact
is observed on phagocytosis and oxidative burst, as well as activation of the
promoter for the pro-inflammatory cytokine TNF-alpha. In contrast, both AgNPs and
Ag+, but not the dendrimer templates, trigger the release of neutrophil
extracellular traps and inhibit the formation of nitric monoxide. On the molecular
level, AgNPs and Ag+ cause elevated intracellular levels of reactive oxygen species
and the second messenger Zn2+. Moreover, protein phosphatases are inhibited by an
oxidative mechanism. Taken together, there are several effects of AgNPs on
neutrophil granulocytes and macrophages in vitro, but these are not specific for
AgNP, instead they are also observed with Ag+, and Ag+ released from AgNPs seems to
be the component responsible for most of the particles' immunomodulatory activity.
AN - rayyan-553780557
AU - Haase, H.
AU - Fahmi, A.
AU - Mahltig, B.
DO - 10.1166/jbn.2014.1784
IS - 6
PY - 2014
SN - 1550-7033 1550-7041
SP - 1146-1156
ST - Impact of Silver Nanoparticles and Silver Ions on Innate Immune Cells
T2 - JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
TI - Impact of Silver Nanoparticles and Silver Ions on Innate Immune Cells
VL - 10
Y2 - 6
ID - 8585
ER -
TY - JOUR
AB - Shigella and Salmonella kill host cells and trigger inflammatory responses by
mechanisms that are not fully understood. The goal of this review is to reevaluate
key observations reported over the past 15 years and, whenever possible, to provide
a chronological perspective as to how our understanding of the pathways by which
Shigella and Salmonella kill host cells has evolved. Published by Elsevier SAS.
AN - rayyan-553780560
AU - Haimovich, B.
AU - Venkatesan, M. M.
DO - 10.1016/j.micinf.2005.08.002
IS - 2
KW - Shigella
PY - 2006
SN - 1286-4579
SP - 568-577
ST - Shigella and Salmonella: death as a means of survival
T2 - MICROBES AND INFECTION
TI - Shigella and Salmonella: death as a means of survival
VL - 8
Y2 - 2
ID - 8588
ER -
TY - JOUR
AB - Biomaterials applications have rapidly expanded into different fields of
sciences. One of the important fields of using biomaterials is dentistry, which can
facilitate implantation, surgery, and treatment of oral diseases such as peri-
implantitis, periodontitis, and other dental problems. Drug delivery systems based
on biocompatible materials play a vital role in the release of drugs into aim
tissues of the oral cavity with minimum side effects. Therefore, scientists have
studied various delivery systems to improve the efficacy and acceptability of
therapeutic approaches in dental problems and oral diseases. Also, biomaterials
could be utilized as carriers in biocompatible drug delivery systems. For instance,
natural polymeric substances, such as gelatin, chitosan, calcium phosphate,
alginate, and xanthan gum are used to prepare different forms of delivery systems.
In addition, some alloys are conducted in drug complexes for the better in
transportation. Delivery systems based on biomaterials are provided with different
strategies, although individual biomaterial has advantages and disadvantages which
have a significant influence on transportation of complex such as solubility in
physiological environments or distribution in tissues. Biomaterials have
antibacterial and anti-inflammatory effects and prolonged time contact and even
enhance antibiotic activities in oral infections. Moreover, these biomaterials are
commonly prepared in some forms such as particulate complex, fibers, microspheres,
gels, hydrogels, and injectable systems. In this review, we examined the
application of biocompatible materials in drug delivery systems of oral and dental
diseases or problems. © 2021 Lotfollah Kamali Hakim et al.
AN - rayyan-553780561
AU - Hakim, L. K.
AU - Yazdanian, M.
AU - Alam, M.
AU - Abbasi, K.
AU - Tebyaniyan, H.
AU - Tahmasebi, E.
AU - Khayatan, D.
AU - Seifalian, A.
AU - Ranjbar, R.
AU - Yazdanian, A.
DO - 10.1155/2021/9011226
KW - alginic acid
antibiotic agent
antiinfective agent
biomaterial
calcium phosphate
chitosan
drug carrier
gel
gelatin
gold nanoparticle
hyaluronic acid
hydrogel
metal nanoparticle
microsphere
silver nanoparticle
stabilizing agent
biocompatibility
bone development
complex formation
human
injection
mouth cavity
mouth disease
mouth infection
nonhuman
Review
tooth disease
PY - 2021
ST - Biocompatible and Biomaterials Application in Drug Delivery System in Oral
Cavity
T2 - Evidence-based Complementary and Alternative Medicine
TI - Biocompatible and Biomaterials Application in Drug Delivery System in Oral
Cavity
85119981141&doi=10.1155%2f2021%2f9011226&partnerID=40&md5=19d3dc6c88b6809ee34422a2f
bd3ab59
VL - 2021
ID - 8589
ER -
TY - JOUR
AB - Periodontal disease is a set of inflammatory conditions affecting the tissues
surrounding the teeth, which can be fairly preventable. In this research,
antibacterial polymeric nanofibers were prepared for the management of periodontal
disease treatment. New hybrid organic/inorganic nanofibers of chitosan/polyethylene
oxide (CS/PEO) contains doxycycline hyclate (DOXH) (as an antibacterial drug) and
silver/hydroxyapatite/silica (Ag/HA/Si) nanocomposite (CS/PEO-DOXH-Ag/HA/Si)
fabricated by electrospinning technique. The nanofibers were characterized by
Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD),
field emission scanning electron microscope (FE-SEM), thermogravimetric analysis
(TGA) and transmission electron microscopy (TEM). In vitro release profile,
degradation (DE) test, swelling (SW) test, antimicrobial activity and cell
viability were also obtained. The DOXH release profile was measured by ultraviolet
visible (UV–Vis) spectrophotometer in artificial saliva (pH 6.8) at 37 °C. The
application of Ag/HA/Si increased the morphological integrity during the
degradation process and decreased the rate of drug delivery. The electrospun
nanofibers showed an enhanced antibacterial activity against Escherichia coli (E.
coli), Staphylococcus aureus (S. aureus), and Streptococcus mutans (S. mutans)
bacterium strains with the lowest DOXH amount, which can reduce the high dosage of
antibiotics and side effects. Cell viability assays revealed that the prepared
nanofibers had no cytotoxicity against the human gingival fibroblast 2 (HGF-2) cell
line. The results suggest that the prepared organic/inorganic hybrid nanofibers
with drug delivery properties could be a promising candidate for the treatment of
periodontal diseases. © 2022, The Author(s), under exclusive licence to Springer-
Verlag GmbH Germany, part of Springer Nature.
AN - rayyan-553780562
AU - Hakimi, F.
AU - Hashemikia, S.
AU - Sadighian, S.
AU - Ramazani, A.
DO - 10.1007/s00289-022-04466-x
IS - 8
KW - Chitosan
Hybrid nanofiber
Local drug delivery
Periodontal disease
Cell culture
Diseases
Drug dosage
Escherichia coli
Nanofibers
Silver
Thermogravimetric analysis
X ray powder diffraction
Antibacterial drugs
Antibacterials
Doxycycline
Inflammatory conditions
Nano-fibrous
Release profiles
Silica composites
Periodontal Diseases
PY - 2023
SP - 8703-8723
ST - Nanofibrous chitosan/polyethylene oxide silver/hydroxyapatite/silica
composite as a potential biomaterial for local treatment of periodontal disease
T2 - Polymer Bulletin
TI - Nanofibrous chitosan/polyethylene oxide silver/hydroxyapatite/silica
composite as a potential biomaterial for local treatment of periodontal disease
85138813253&doi=10.1007%2fs00289-022-04466-
x&partnerID=40&md5=46120c5fd3fd271d43ff5140b0d5c62b
VL - 80
ID - 8590
ER -
TY - JOUR
AB - BACKGROUND: Anthrax is caused by Bacillus anthracis that produce two
exotoxins, lethal toxin and edema toxin. The lethal toxin is composed of the lethal
factor (LF) complexed with the cell binding protective antigen (PA83, 83 kDa).
Likewise, the edema factor (EF) binds to the PA83 to form the edema toxin. Once
PA83 is bound to the host cell surface, a furin-like protease cleaves the full-
length, inactive protein into 63 kDa and 20 kDa antigens (PA63 and PA20). PA63
forms a heptamer and is internalized via receptor mediated endocytosis forming a
protease-stable pore, which allows EF and LF to enter the cell and exert their
toxic effects.Both proteolytically cleaved protective antigens (PA63 and PA20
fragments) are found in the blood of infected animals. The 63 kDa protective
antigen PA63 fragment has been thoroughly studied while little is known about the
PA20. METHODS: In this study we examined the role of PA20 using high throughput
gene expression analysis of human peripheral blood mononuclear cells (PBMC) exposed
to the PA20. We constructed a PA mutant in which a Factor Xa proteolytic
recognition site was genetically engineered into the protective antigen PA83 to
obtain PA20 using limited digestion of this recombinant PA83 with trypsin. RESULTS:
Global gene expression response studies indicated modulation of various immune
functions and showed gene patterns indicative of apoptosis via the Fas pathway in a
subset of the lymphoid cells. This finding was extended to include observations of
increased Caspase-3 enzymatic activity and the identification of increases in the
population of apoptotic, but not necrotic cells, based on differential staining
methods. We identified a list of approximately 40 inflammatory mediators and heat-
shock proteins that were altered similarly upon exposure of PBMC to either rPA20 or
B. anthracis spores/vegetative cells. CONCLUSION: This study shows that the PA20
has an effect on human peripheral blood leukocytes and can induce apoptosis in the
absence of other PA components.
AN - rayyan-553782127
AU - Hammamieh, R.
AU - Ribot, W. J.
AU - Abshire, T. G.
AU - Jett, M.
AU - Ezzell, J.
DO - 10.1186/1471-2334-8-124
J2 - BMC Infect Dis
KW - ADP-ribosyl Cyclase 1/immunology
Anthrax/*immunology/microbiology
Antigens, Bacterial/immunology/*metabolism
Apoptosis
Bacillus anthracis/genetics/immunology/*metabolism
Bacterial Proteins/immunology/metabolism
Bacterial Toxins/immunology/*metabolism
Caspase 3/immunology
Gene Expression Profiling
Humans
Leukocytes, Mononuclear/*immunology/microbiology
Recombinant Proteins/immunology/metabolism
fas Receptor/immunology
Bacillus anthracis
LA - eng
N1 - Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
rasha.hammamieh1@us.army.mil
PY - 2008
SP - 124
ST - Activity of the Bacillus anthracis 20 kDa protective antigen component
T2 - BMC infectious diseases
TI - Activity of the Bacillus anthracis 20 kDa protective antigen component
VL - 8
Y2 - 9 y3 - 22
ID - 10038
ER -
TY - JOUR
AB - The present study was conducted to evaluate the antitumor effect of silver
nanoparticles and Alpha-lipoic acid and their combination against 1,2-
dimethylhydrazine (DMH-) induced colorectal carcinogenesis in experimental rats.
Fifty Male wistar albino rats were divided into five groups. Group (1): negative
control group, group (2) colorectal cancer induced rats (CRC) (positive control),
group (3): CRC group treated with silver nanoparticles (CRC+AgNPs), group (4): CRC
induced rats treated with Alpha-lipoic acid (CRC+ALA) and group (5): CRC induced
rats treated with combinations (CRC+AgNPs + ALA). The obtained biochemical results
revealed significant reduction in serum transforming growth factor-beta (TGF-beta),
Tumor necrosis factor alpha(TNF-alpha), C - Reactive Protein (CRP),
carcinoembryonic antigen (CEA) and colon cancer specific antigen-4 (CCSA-4) levels
in CRC induced group treated either with silver nanoparticles or alpha-lipoic acid
and their combinations compared to CRC induced group. Regarding gene expression, we
found significant down regulation in K-RAS gene expression with treatment with
either AgNps or ALA or their combinations comparing to untreated group.
Histopathological study greatly supports these results. In Conclusion; the present
study highlighted the antitumor effect of AgNPs, ALA and their Combination in
ameliorating CRC induced experimentally through its potential anti-inflammatory
effect. Copyright (C) 2013 - All Rights Reserved - Pharmacophore
AN - rayyan-553780564
AU - Hamza, A.
AU - AlSolami, F.
IS - 2
KW - Thioctic Acid
PY - 2018
SN - 2229-5402
SP - 45-51
ST - ANTITUMOR ACTIVITY OF SILVER NANOPARTICLES AND ALPHA-LIPOIC ACID COMBINATIONS
IN COLORECTAL CANCER INDUCED EXPERIMENTALLY
T2 - PHARMACOPHORE
TI - ANTITUMOR ACTIVITY OF SILVER NANOPARTICLES AND ALPHA-LIPOIC ACID COMBINATIONS
IN COLORECTAL CANCER INDUCED EXPERIMENTALLY
VL - 9
Y2 - 3
ID - 8591
ER -
TY - JOUR
AB - Biocomposite nanomaterials have been evolved as the new generation catalysts
and therapeutic supplement in these days. Magnetically isolation has added new
features to this category. This has encouraged us to synthesize a novel Ag NP
adorned chitosan-alginate dual bio-polysaccharide (two of the more versatile
polysaccharides) modified core-shell magnetic nanocomposite (Fe3O4/CS-Alg/Ag NPs).
The material was meticulously characterized following different physicochemical
techniques, such as, FT-IR, ICP-OES, FESEM, EDX, atomic mapping, TEM, VSM, XRD and
XPS studies. The as synthesized material was catalytically explored in the one-pot
multicomponent synthesis of biologically potent 2H-indazolo[2,1-b]phthalazine-
trione derivatives involving a wide range of substrates. The reactions were ended
up with excellent yields under solvent-free heating conditions. The catalyst
recyclability, heterogeneity and leaching tests were performed to ensure its high
stability and robustness. It could be reused as much as 10 times in succession with
almost unchanged catalytic performances. In the lung protective part of the present
research, the human lung toxicity was induced by α-Guttiferin. The cell viability
of lung MRC-5, CCD[sbnd]19Lu, WI-38, and BEAS-2B cell lines was measured by trypan
blue assay. Caspase-3 activity was assessed by the caspase activity colorimetric
assay kit and mitochondrial membrane potential of lung cells was studied by
Rhodamine123 fluorescence dye. Terminal deoxynucleotidyl transferase dUTP nick end
labeling (TUNEL) test was used to show DNA fragmentation and apoptosis of lung
cells. Also, the Rat inflammatory cytokine assay kit was used to measure the
concentrations of inflammatory cytokines. The catalyst-treated cell cutlers
significantly (p ≤ 0.01) reduced the DNA fragmentation, caspase-3 activity, and
inflammatory cytokines concentrations, and raised the mitochondrial membrane
potential and cell viability in the high concentration of α-Guttiferin-treated lung
MRC-5, CCD[sbnd]19Lu, WI-38, and BEAS-2B cells. The best result of lung protective
properties of catalyst against α-Guttiferin was seen in the high dose of catalyst
i.e., 4 μg. DPPH test revealed similar antioxidant potentials for catalyst and
butylated hydroxytoluene. The catalyst inhibited half of the DPPH molecules in the
concentration of 171 μg/mL. According to the above results, catalyst can be
administrated as a lung protective drug for the treatment of lung diseases after
approving in the clinical trial studies in humans. © 2020 Elsevier B.V.
AN - rayyan-553780566
AU - Han, Y.
AU - Gao, Y.
AU - Cao, X.
AU - Liu, S.
AU - Li, J.
DO - 10.1016/j.ijbiomac.2020.08.183
KW - 3drug
Ag nanoparticles
Chitosan-alginate
Indazolophthalazine-triones
Magnetic nanocomposite
α-Guttiferin
Alginates
Animals
Catalysis
Cell Line
Cell Survival
Chitosan
Cytokines
Humans
Indazoles
Lung
Magnetite Nanoparticles
Membrane Potential, Mitochondrial
Phthalazines
Rats
Silver
Umbelliferones
alginic acid
alpha guttiferin
caspase 3
chemical compound
chitosan
cresol
indazolo[2,1 b]phthalazine
interleukin 1alpha
interleukin 1beta
interleukin 6
magnetite
nanocomposite
rhodamine 123
silver nanoparticle
unclassified drug
cytokine
indazole derivative
magnetite nanoparticle
phthalazine derivative
silver
umbelliferone derivative
apoptosis
Article
BEAS-2B cell line
catalyst
cell viability
colorimetry
controlled study
cytokine release
DNA fragmentation
enzyme activity
heating
human
human cell
lung toxicity
MRC-5 cell line
one pot synthesis
physical chemistry
TUNEL assay
vibrating sample magnetometry
WI-38 cell line
X ray diffraction
animal
catalysis
cell line
cell survival
chemistry
cytology
drug effect
immunology
lung
metabolism
rat
Humanities
Humanism
Aged
Aging
PY - 2020
SP - 2974-2986
ST - Ag NPs on chitosan-alginate coated magnetite for synthesis of indazolo[2,1-
b]phthalazines and human lung protective effects against α-Guttiferin
TI - Ag NPs on chitosan-alginate coated magnetite for synthesis of indazolo[2,1-
b]phthalazines and human lung protective effects against α-Guttiferin
85090020864&doi=10.1016%2fj.ijbiomac.2020.08.183&partnerID=40&md5=b7503dbbcd15c06b9
2dbf5ce7f4bca2b
VL - 164
ID - 8593
ER -
TY - JOUR
AB - Hepatocellular carcinoma is one of the most common lethal diseases worldwide
and there is no effective treatment till date. Natural products derived from the
plants play an important role in chemoprevention and act as therapeutic antitumor
agents. Licorice is a plant that has been used in food and medicine for the
treatment of various diseases. 18 beta-Glycyrrhetinic acid (18 beta-GA), a
pentacyclic triterpenoid obtained from the roots of licorice plant, is reported to
possess various pharmacological properties such as antitumor and antiinflammatory
activities. The present study was designed to elucidate the chemopreventive effect
of 18 beta-GA through antiinflammation, antiproliferation, and induction of
apoptosis in human hepatoma cell line HepG2. 18 beta-GA significantly inhibits the
proliferation of HepG2 cell without affecting the normal liver cell line (Chang's).
In the present study, 18 beta-GA increased the formation of reactive oxygen
species, nitric oxide production, and loss of mitochondrial membrane potential,
suggesting the involvement of 18 beta-GA in apoptosis which was also confirmed by
assessing the markers involved in apoptosis like caspase-3, caspase-9, Bax:Bcl-2
ratio, and cleaved PARP. 18 beta-GA also downregulated the expression of
inflammatory proteins such as NF-kappa B, iNOS, and COX-2. Keeping these data into
consideration, our results suggest that 18 beta-GA may be used as a chemopreventive
agent in liver cancer.
AN - rayyan-553780569
AU - Hasan, S. K.
AU - Siddiqi, A.
AU - Nafees, S.
AU - Ali, N.
AU - Rashid, S.
AU - Ali, R.
AU - Shahid, A.
AU - Sultana, S.
DO - 10.1007/s11010-016-2705-2
IS - 1
KW - Humanities
Humanism
Humans
Chemoprevention
Cell Line
PY - 2016
SN - 0300-8177 1573-4919
SP - 169-177
ST - Chemopreventive effect of 18 beta-glycyrrhetinic acid via modulation of
inflammatory markers and induction of apoptosis in human hepatoma cell line (HepG2)
T2 - MOLECULAR AND CELLULAR BIOCHEMISTRY
TI - Chemopreventive effect of 18 beta-glycyrrhetinic acid via modulation of
inflammatory markers and induction of apoptosis in human hepatoma cell line (HepG2)
VL - 416
Y2 - 5
ID - 8596
ER -
TY - JOUR
AB - Nanoparticles (NPs) are currently the focus of considerable attention for
dental applications; however, their biological effects have not been fully
elucidated. The long-term, slow release of matrix metalloproteases (MMPs) digests
collagen fibrils within resin-dentin bonds. Therefore, MMP inhibitors can prolong
the durability of resin-dentin bonds. However, there have been few reports
evaluating the combined effect of MMP inhibition and the cytotoxic effects of NPs
for dentin bonding. The aim of this study was to evaluate MMP inhibition and
cytotoxic responses to gold (AuNPs) and platinum nanoparticles (PtNPs) stabilized
by polyvinylpyrrolidone (PVP) in cultured murine macrophages (RAW264) by using MMP
inhibition assays, measuring cell viability and inflammatory responses
(quantitative reverse transcription polymerase chain reaction [RT-qPCR]), and
conducting a micromorphological analysis by fluorescence and transmission electron
microscopy. Cultured RAW264 cells were exposed to metal NPs at various
concentrations (1, 10, 100, and 400 mu g/mL). AuNPs and PtNPs markedly inhibited
MMP-8 and MMP-9 activity. Although PtNPs were cytotoxic at high concentrations (100
and 400 mu g/mL), no cytotoxic effects were observed for AuNPs at any
concentration. Transmission electron microscopy images showed a significant
nonrandom intercellular distribution for AuNPs and PtNPs, which were mostly
observed to be localized in lysosomes but not in the nucleus. RT-qPCR analysis
demonstrated inflammatory responses were not induced in RAW264 cells by AuNPs or
PtNPs. The cytotoxicity of nanoparticles might depend on the core metal composition
and arise from a "Trojan horse" effect; thus, MMP inhibition could be attributed to
the surface charge of PVP, which forms the outer coating of NPs. The negative
charge of the surface coating of PVP binds to Zn2+ from the active center of MMPs
by chelate binding and results in MMP inhibition. In summary, AuNPs are attractive
NPs that effectively inhibit MMP activity without cytotoxicity or inflammatory
responses.
AN - rayyan-553780571
AU - Hashimoto, M.
AU - Sasaki, J. I.
AU - Yamaguchi, S.
AU - Kawai, K.
AU - Kawakami, H.
AU - Iwasaki, Y.
AU - Imazato, S.
DO - 10.1177/0022034515589282
IS - 8
PY - 2015
SN - 0022-0345 1544-0591
SP - 1085-1091
ST - Gold Nanoparticles Inhibit Matrix Metalloproteases without Cytotoxicity
T2 - JOURNAL OF DENTAL RESEARCH
TI - Gold Nanoparticles Inhibit Matrix Metalloproteases without Cytotoxicity
VL - 94
Y2 - 8
ID - 8598
ER -
TY - JOUR
AB - This study evaluated the inhibition of matrix metalloproteases (MMPs) and
cellular responses elicited by gold (Au) and platinum (Pt) nanoparticles (NPs). The
interaction of MMP-1 and NPs was evaluated using an MMP assay kit. The cultured
L929 cells were exposed to various concentrations of NPs. The cellular responses to
NPs were examined using a cytotoxicity assay (that evaluated cell viability and
lactic dehydrogenase production), real-time polymerase chain reaction (RT-qPCR),
and transmission electron microscopy. Both types of NPs, when used at
concentrations above 10 g ml(-1), inhibited MMP-1 activity. No cytotoxic effects
were found when the cells were exposed to AuNPs. In contrast, PtNPs, at both 100
and 400 g ml(-1), induced cytotoxicity. No inflammatory responses (production of
interleukin-6 and tumor necrosis factor-alpha) to NPs were identified by RT-qPCR.
The negative surface charge of NPs (COOH-) binds to the Zn2+ of the MMP active
center by chelation, leading to MMP inhibition. Gold nanoparticles are plausible
candidates for MMP inhibitors in resin-bonding materials because they effectively
inhibit MMP-1 activity without cytotoxic or inflammatory effects.
AN - rayyan-553780574
AU - Hashimoto, M.
AU - Yamaguchi, S.
AU - Sasaki, J. I.
AU - Kawai, K.
AU - Kawakami, H.
AU - Iwasaki, Y.
AU - Imazato, S.
DO - 10.1111/eos.12235
IS - 1
PY - 2016
SN - 0909-8836 1600-0722
SP - 68-74
ST - Inhibition of matrix metalloproteinases and toxicity of gold and platinum
nanoparticles in L929 fibroblast cells
T2 - EUROPEAN JOURNAL OF ORAL SCIENCES
TI - Inhibition of matrix metalloproteinases and toxicity of gold and platinum
nanoparticles in L929 fibroblast cells
VL - 124
Y2 - 2
ID - 8601
ER -
TY - JOUR
AB - Objectives The first goal of this study was to synthesize the silver
nanoparticles Alcaligenes xylosoxidans exopolysaccharide (Ag-AXEPS). The second
objective was to analyse the role of Ag-AXEPS nanoparticles (NPS) in treating
bleomycin (BLM)-induced lung fibrosis. Methods Intratracheal bleomycin (2.5 U/kg)
was administered to prompt pulmonary fibrosis in rats, and pulmonary fibrosis was
treated with Ag-AXEPS nanoparticles (100 ppm/twice a week for four weeks). Key
findings Ag-AXEPS nanoparticles significantly decreased the diversity of pulmonary
inflammatory agents in rats with BLM-induced fibrosis. Reduced levels of
respiratory tumor necrosis factor-alpha, monocyte chemotactic protein-1, matrix
metalloproteinases (MMP-2 and MMP-9) were observed on treatment with synthesized
Ag-AXEPS. Similarly, the treatment decreased IL-12, mRNA levels of BAX and plasma
fibrosis markers like N-terminal procollagen III propeptide and transforming growth
factor-β1. On the other hand, the treatment increased mRNA BCL2 and total
antioxidant capacity. It also lowered the level of fibrosis, as was shown by a
quantified pathologic study of hematoxylin–eosin-stained lung parts.The treatment,
however, ensured that lung collagen was restored, as assessed by Masson’s trichrome
stain, and that overall survival was increased and enhanced. Conclusions: Our work
showed that nanoparticles could be obtained at 37°C and may be a possible pulmonary
fibrosis therapeutic agent. © The Author(s) 2021. Published by Oxford University
Press on behalf of the Royal Pharmaceutical Society. All rights reserved.
AN - rayyan-553780575
AU - Hassan, A. I.
AU - Samir, A.
AU - Youssef, H. F.
AU - Mohamed, S. S.
AU - Asker, M. S.
AU - Mahmoud, M. G.
DO - 10.1093/jpp/rgab037
IS - 11
KW - apoptosis
exopolysaccharides
Masson’s trichrome
matrix metalloproteinases
pulmonary fibrosis
Alcaligenes
Animals
Antibiotics, Antineoplastic
Antioxidants
Apoptosis
bcl-2-Associated X Protein
Bleomycin
Collagen
Fibrosis
Inflammation
Lung
Male
Matrix Metalloproteinases
Metal Nanoparticles
Nanoconjugates
Nanoparticles
Pneumonia
Polysaccharides
Polysaccharides, Bacterial
Proto-Oncogene Proteins c-bcl-2
Pulmonary Fibrosis
Silver
Transforming Growth Factor beta1
bleomycin
collagen
eosin
exopolysaccharide
gelatinase A
gelatinase B
hematoxylin
interleukin 12
messenger RNA
nanocarrier
procollagen type 3 aminopropeptide
protein Bax
protein bcl 2
respiratory tract agent
silver nanoparticle
antineoplastic antibiotic
antioxidant
bacterial polysaccharide
matrix metalloproteinase
metal nanoparticle
nanoconjugate
nanoparticle
polysaccharide
silver
Tgfb1 protein, rat
Achromobacter xylosoxidans
adult
animal experiment
animal model
animal tissue
Article
bleomycin-induced pulmonary fibrosis
controlled study
drug effect
lung fibrosis
male
Masson staining
nonhuman
overall survival
rat
synthesis
treatment duration
animal
fibrosis
inflammation
lung
metabolism
pathology
pneumonia
Sprague Dawley rat
Rats
PY - 2021
SP - 1503-1512
ST - Effects of silver nanoparticles–polysaccharide on bleomycin-induced pulmonary
fibrosis in rats
T2 - Journal of Pharmacy and Pharmacology
TI - Effects of silver nanoparticles–polysaccharide on bleomycin-induced pulmonary
fibrosis in rats
85118096853&doi=10.1093%2fjpp
%2frgab037&partnerID=40&md5=1627596c4e9794f645c28499fb092a5b
VL - 73
ID - 8602
ER -
TY - JOUR
AB - Background: Pomegranate (Punica granatum L) has been used since ancient times
in the traditional medicine of several cultures, particularly in the Middle East.
It is an essential commercial crop full of bioactive compounds with several medical
applications. Pomegranate is very popular for its biological effects exerted by
phenolic compounds via free radical scavenging abilities. It has revealed high
antioxidant and anti-inflammatory activities and is beneficial for the amelioration
of liver and kidney diseases. Purpose: To elucidate the potential efficacy of
pomegranate juice (PJ) against copper oxide nanoparticles (CuO-NPs)-induced
apoptosis, inflammation, and oxidative stress damage. Study design: 37 nm sized
CuO-NPs were prepared by precipitation method and characterized by using X-ray
diffractometer (XRD), Zetasizer nano-and high-resolution transmission electron
microscope (HR-TEM). 30 Wistar rats were partitioned into 6 equal groups as
follows: Group 1 (negative control), groups 2 & 3 (PJ control groups), group 4
(CuO-NPs group), groups 5 & 6 (CuO-NPs + PJ groups). Methods: Hepato-renal
protective effect of PJ was evaluated by measuring levels of serum marker enzymes
(ALT, AST,blood urea nitrogen and creatinine). Cu NPs bioaccumulation in liver and
kidneys was determined by using atomic absorption spectrophotometer. The oxidative
stress markers, Rt-PCR analysis, histopathological and immunohistochemical studies
were carried out in the liver and kidneys to support the above parameters. Results:
Rats injected with CuO-NPs showed higher levels of the above serum marker enzymes,
alteration of oxidant-antioxidant balance together with severe pathological
alterations in liver and kidney tissues and overexpression of both caspase-3 and
nuclear factor kappa B protein (NF-kappa B) associated with upregulation of Box
gene and downregulation of Bcl2 gene in these organs. PJ ameliorated all of the
above toxicological parameters. Conclusion: PJ was proved to be a potential hepato-
renal protective agent against liver and kidney damage induced by CuO-NPs via its
antioxidant, anti-inflammatory, and anti-apoptotic effects.
AN - rayyan-553780578
AU - Hassanen, E. I.
AU - Tohamy, A. F.
AU - Issa, M. Y.
AU - Ibrahim, M. A.
AU - Farroh, K. Y.
AU - Hassan, A. M.
DO - 10.2147/IJN.S229461
KW - Mitochondria
NF-kappa B
Signal Transduction
Rats
PY - 2019
SN - 1178-2013
SP - 8905-8922
ST - Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB
Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys Of
Rats
TI - Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB
Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys Of
Rats
VL - 14
ID - 8605
ER -
TY - JOUR
AB - Brucella spp. are facultative intracellular bacteria that cause brucellosis
in humans and other animals. Brucella spp. are taken up by macrophages, and the
outcome of the macrophage-Brucella interaction is a basis for establishment of a
chronic Brucella infection. Microarrays were used to analyze the transcriptional
response of the murine macrophage-like J774.A1 cell line to infection with virulent
Brucella melitensis strain 16M. It was found that most significant changes in
macrophage gene transcription happened early following infection, and global
macrophage gene expression profiles returned to normal between 24 and 48 h
postinfection. These findings support the observation that macrophages kill the
majority of Brucella cells at the early infection stage, but the surviving Brucella
cells are able to avoid macrophage brucellacidal activity inside replicative
phagosomes at the later infection stage. At 4 h postinfection, macrophage genes
involved in cell growth, metabolism, and responses to endogenous stimuli were down-
regulated, while the inflammatory response (e.g., tumor necrosis factor alpha and
Toll-like receptor 2), the complement system, the responses to external stimuli,
and other immune responses were up-regulated. It is likely that the most active
brucellacidal activity happened between 0 and 4 111 postinfection. Mitochondrion-
associated gene expression, which is involved in protein synthesis and transport,
electron transfer, and small-molecule transfer, and many other mitochondrial
functions were significantly down-regulated at 4 h postinfection. Although there
were both pro- and antiapoptosis effects, B. melitensis 16M appears to inhibit
apoptosis of macrophages by blocking release of cytochrome c and production of
reactive oxygen species in the mitochondria, thus preventing activation of caspase
cascades.
AN - rayyan-553780580
AU - He, Y. Q.
AU - Reichow, S.
AU - Ramamoorthy, S.
AU - Ding, X. C.
AU - Lathigra, R.
AU - Craig, J. C.
AU - Sobral, B. W. S.
AU - Schurig, G. G.
AU - Sriranganathan, N.
AU - Boyle, S. M.
DO - 10.1128/IAI.01998-05
IS - 9
Mitochondria
Macrophages
Brucella
Brucella melitensis
PY - 2006
SN - 0019-9567 1098-5522
SP - 5035-5046
ST - Brucella melitensis triggers time-dependent modulation of a apoptosis and
down-regulation of mitochondrion-associated gene expression in mouse macrophages
T2 - INFECTION AND IMMUNITY
TI - Brucella melitensis triggers time-dependent modulation of a apoptosis and
down-regulation of mitochondrion-associated gene expression in mouse macrophages
VL - 74
Y2 - 9
ID - 8607
ER -
TY - JOUR
AB - Rationale: Humans with a dominant negative mutation in STAT3 are susceptible
to severe skin infections, suggesting an essential role for STAT3 signaling in
defense against cutaneous pathogens. Methods: To focus on innate antiviral defenses
in keratinocytes, we used a standard model of cutaneous infection of severe
combined immunodeficient mice with the current smallpox vaccine, ACAM-2000. In
parallel, early events post-infection with the smallpox vaccine ACAM-2000 were
investigated in cultured keratinocytes of human and mouse origin. Results: Mice
treated topically with a STAT3 inhibitor (Stattic) developed larger vaccinia
lesions with higher virus titers and died more rapidly than untreated controls.
Cultured human and murine keratinocytes infected with ACAM-2000 underwent rapid
necrosis, but when treated with Stattic or with inhibitors of RIP1 kinase or
caspase-1, they survived longer, produced higher titers of virus, and showed
reduced activation of type I interferon responses and inflammatory cytokines
release. Treatment with inhibitors of RIP1 kinase and STAT3, but not caspase-1,
also reduced the inflammatory response of keratinocytes to TLR ligands. Vaccinia
growth properties in Vero cells, which are known to be defective in some antiviral
responses, were unaffected by inhibition of RIP1K, caspase-1, or STAT3.
Conclusions: Our findings indicate that keratinocytes suppress the replication and
spread of vaccinia virus by undergoing rapid programmed cell death, in a process
requiring STAT3. These data offer a new framework for understanding susceptibility
to skin infection in patients with STAT3 mutations. Interventions which promote
prompt necroptosis/pyroptosis of infected keratinocytes may reduce risks associated
with vaccination with live vaccinia virus.
AN - rayyan-553780581
AU - He, Y.
AU - Fisher, R.
AU - Chowdhury, S.
AU - Sultana, I.
AU - Pereira, C. P.
AU - Bray, M.
AU - Reed, J. L.
DO - 10.1371/journal.pone.0113690
IS - 11
KW - Vaccinia virus
Keratinocytes
PY - 2014
SN - 1932-6203
ST - Vaccinia Virus Induces Rapid Necrosis in Keratinocytes by a STAT3-Dependent
Mechanism
T2 - PLOS ONE
TI - Vaccinia Virus Induces Rapid Necrosis in Keratinocytes by a STAT3-Dependent
Mechanism
VL - 9
Y2 - 11 y3 - 24
ID - 8608
ER -
TY - JOUR
AB - Recently, the development of skin barrier depend on wound healing, which is
one of the most complicated biological processes. As an alternative to conventional
antibiotics, nanoparticles (NPs) have become more utilized generally to attack
bacteria. Due to their distinct characteristics, potential microbicidal action, and
ability to speed up the wound healing process, zinc oxide nanoparticles (ZnO-NPs)
have attracted much attention. Biological techniques can solve the restrictions of
both physical and chemical approaches for nanoparticles synthesis. Because it does
not require expensive chemicals, high temperatures, or a lot of time, biological
synthesis is relatively easy, inexpensive, and environmentally benign. The
secondary metabolic extract from Escherichia coli was used in this study to
biologically synthesize three distinct quantities of ZnO-NPs, which were then
assessed for their effectiveness in wound healing and bacterial infection
prevention. The biofabricated ZnO-NPs were fully characterized in terms of particle
shape, morphology, and stability against aggregation. Depending on the
concentration of the utilized zinc salt, three different samples were fabricated
biologically, nominated as ZnO-NPs-1, ZnO-NPs-2, and ZnO-NPs-3. The findings of Uv-
vis absorption peaks were obtained at 352 nm, demonstrating the preparation of ZnO-
NPs. The results demonstrated the formation of ZnO-NPs with an average particle
size of 79.19, 79.83 and 91.57 nm for the three prepared samples (ZnO-NPs-1, ZnO-
NPs-2, and ZnO-NPs-3), respectively. Additionally, these samples of ZnO-NPs
exhibited zeta potential values around -34.3, -33.7, and -33.4 mV, respectively.
Energy dispersive X-ray confirmed the successful formation of ZnO-NPs. It was also
observed from the obtained results that, ZnO-NP-3 showed superior antimicrobial
potential against selected skin infectious microbes. The effective killing dosage
of ZnO-NPs-3 was recorded to be 40 mg/L which can eliminate microbial growth. The
dysregulation of skin flora significantly influences the etiology of inflammatory
skin disorders.
AN - rayyan-553780582
AU - Hemdan, B. A.
AU - El-Naggar, M. E.
AU - Abd-Elgawad, S. E.
AU - El Zawawy, N. A.
AU - Mahmoud, Y. A. G.
DO - 10.1007/s13399-023-04313-7
KW - Bacterial Infections
PY - 2023
SN - 2190-6815 2190-6823
ST - Bacterial cell-free metabolites-based zinc oxide nanoparticles for combating
skin-causing bacterial infections
T2 - BIOMASS CONVERSION AND BIOREFINERY
TI - Bacterial cell-free metabolites-based zinc oxide nanoparticles for combating
skin-causing bacterial infections
Y2 - 5 y3 - 29
ID - 8609
ER -
TY - JOUR
AB - Fragaria ananassa, also known as "Strawberry" is a common species in Iran and
widely used for its anti-inflammatory, anti-ulcer, astringent, anti-allergic,
antibacterial, antifungal, and antidiarrheal activities and also in the treatment
of skin wounds. The purpose of the study was chemical characterization and
assessment of cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous
wound healing properties of copper nanoparticles (CuNPs) using the aqueous extract
of Strawberry fruit and L-Ascorbic acid as reducing and stabilizing agents. These
nanoparticles were characterized by FT-IR, UV-visible spectroscopy, EDS, FE-SEM,
and TEM analysis. TEM images exhibited a uniform spherical morphology and diameters
of 10-30 nm for the biosynthesized nanoparticles. DPPH free radical scavenging test
revealed similar antioxidant properties for Strawberry, CuNPs, and butylated
hydroxytoluene. The Strawberry and synthesized CuNPs had great cell viability dose-
dependently against HUVEC cell line. In the microbiological part of this study,
CuNPs showed higher antibacterial and antifungal properties than all standard
antibiotics (p <= 0.01). Also, CuNPs prevented the growth of all bacteria at 2-8
mg/mL concentrations and destroyed them at 2-16 mg/mL concentrations (p <= 0.01).
In the case of antifungal property of CuNPs, they inhibited the growth of all fungi
at 2-4 mg/mL concentrations and destroyed them at 2-8 mg/mL concentrations (p <=
0.01). In vivo design, the use of CuNPs ointment in the treatment groups
substantially remarkably raised (p <= 0.01) the wound contracture, hydroxyl
proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and
reduced (p <= 0.01) the wound area, total cells, neutrophil, macrophage, and
lymphocyte compared to Strawberry, CuSO4, tetracycline, Eucerin basal, and
untreated control groups. In conclusion, the results of chemical characterization
confirm that the Strawberry fruit can be consumed to produce copper nanoparticles
with a remarkable amount of remedial effects without any cytotoxicity against
HUVECs. (C) 2020 Elsevier Ltd. All rights reserved.
AN - rayyan-553780583
AU - Hemmati, S.
AU - Ahmeda, A.
AU - Salehabadi, Y.
AU - Zangeneh, A.
DO - 10.1016/j.poly.2020.114425
KW - Ascorbic Acid
Wound Healing
Antioxidants
PY - 2020
SN - 0277-5387 1873-3719
ST - Synthesis, characterization, and evaluation of cytotoxicity, antioxidant,
antifungal, antibacterial, and cutaneous wound healing effects of copper
nanoparticles using the aqueous extract of Strawberry fruit and L-Ascorbic acid
T2 - POLYHEDRON
TI - Synthesis, characterization, and evaluation of cytotoxicity, antioxidant,
antifungal, antibacterial, and cutaneous wound healing effects of copper
nanoparticles using the aqueous extract of Strawberry fruit and L-Ascorbic acid
VL - 180
Y2 - 4 y3 - 1
ID - 8610
ER -
TY - JOUR
AB - The recent study reveals the cytotoxicity, antioxidant, and anti-acute
myeloid leukemia properties of polydopamine (PDA)-capped silver nanoparticles
(AgNPs) compared to doxorubicin in a leukemic mouse model. The synthesized AgNPs
were characterized using different techniques including ultraviolet–visible
spectroscopy (UV–Vis.), Fourier-transform infrared spectroscopy (FT-IR)
spectroscopy, X-ray diffraction (XRD), energy Dispersive X-ray Spectrometry (EDS),
field emission-scanning electron microscopy (FE-SEM), and transmission electron
microscopy (TEM). FE-SEM and TEM images revealed a uniform spherical morphology and
average diameters of 15–25 nm for the nanoparticles. Also, in XRD analysis, ~20 nm
was measured for the crystal size of nanoparticles. The results of the biological
experiments were fed into SPSS-22 software and analyzed by one-way ANOVA. In the
biological in vitro part of this study, AgNPs similar to doxorubicin had low cell
viability dose-dependently against Human HL-60/vcr, 32D-FLT3-ITD, and Murine C1498
cell lines without any cytotoxicity on HUVEC cell line. In this study, 2,2-
diphenyl-1-picrylhydrazyl (DPPH) test revealed similar antioxidant potentials for
doxorubicin and AgNPs. In the biological in vivo part of this study, induction of
acute myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene (DMBA) in 75
mice. Then, the animals were randomly divided into six subgroups, including
control, untreated, doxorubicin, AgNPs, PDA, and AgNO3. AgNPs similar to
doxorubicin, significantly (p ≤ 0.05) reduced the pro-inflammatory cytokines, and
the total white blood cell (WBC), blast, monocyte, neutrophil, eosinophil, and
basophil counts and enhanced the anti-inflammatory cytokines and the platelet,
lymphocyte, and red blood cell (RBC) parameters as compared to the untreated mice.
By quantitative real-time polymerase chain reaction (PCR), sphingosine-1-phosphate
receptor-1 (S1PR1) and sphingosine-1-phosphate receptor-5 (S1PR5) mRNA expression
in lymphocytes were significantly (p ≤ 0.05) raised by treating the leukemic mice
with the AgNPs and doxorubicin. Above results confirm the therapeutic effects of
AgNPs on acute myeloid leukemia in the leukemic mice. Further clinical trials are
necessary for confirmation these remedial properties of AgNPs in human. © 2019 John
Wiley & Sons, Ltd.
AN - rayyan-553780584
AU - Hemmati, S.
AU - Joshani, Z.
AU - Zangeneh, A.
DO - 10.1002/aoc.5277
IS - 2
doxorubicin
leukemic mouse model
polydopamine
Alkanolamines
Antioxidants
Biochemistry
Blood
Cell culture
Diseases
Enamels
Field emission microscopes
Lymphocytes
Mammals
Metal nanoparticles
Silver compounds
Sphingosines
X ray diffraction
Doxorubicin
Energy dispersive x-ray spectrometries (EDS)
Field emission scanning electron microscopy
Fourier transform infra red (FTIR) spectroscopy
Mouse models
Polydopamine
Quantitative real-time polymerase chain reaction
Leukemia, Myeloid
Mice
PY - 2020
ST - Biosynthesis and chemical characterization of polydopamine-capped silver
nanoparticles for the treatment of acute myeloid leukemia in comparison to
doxorubicin in a leukemic mouse model
TI - Biosynthesis and chemical characterization of polydopamine-capped silver
nanoparticles for the treatment of acute myeloid leukemia in comparison to
doxorubicin in a leukemic mouse model
85076193172&doi=10.1002%2faoc.5277&partnerID=40&md5=584e5f6eca176d44de04fe8390fffde
9
VL - 34
ID - 8611
ER -
TY - JOUR
AB - Phosphorylase kinase (PhK) is a unique enzyme in which the spatial
arrangements of the specificity determinants can be manipulated to allow the enzyme
to recognize substrates of different specificities. In this way, PhK is capable of
transferring high energy phosphate bonds from ATP to serine/threonine and tyrosine
moieties in serine/threonine kinases and tyrosine kinases, thus playing a key role
in the activation of multiple signaling pathways. Phosphorylase kinase is released
within five minutes following injury and is responsible for activating inflammatory
pathways in injury-activated scarring following burns. In photo-damaged skin, PhK
plays an important role in promoting photocarcinogenesis through activation of NF-
kB-dependent signaling pathways with inhibition of apoptosis of photo-damaged
cells, thus promoting the survival of precancerous cells and allowing for
subsequent tumor transformation. Curcumin, the active ingredient in the spice,
turmeric, is a selective and non-competitive PhK inhibitor. By inhibition of PhK,
curcumin targets multiple PhK-dependent pathways, with salutary effects on a number
of skin diseases induced by injury. In this paper, we show that curcumin gel
produces rapid healing of burns, with little or no residual scarring. Curcumin gel
is also beneficial in the repair of photo-damaged skin, including pigmentary
changes, solar elastosis, thinning of the skin with telangiectasia (actinic
poikiloderma), and premalignant lesions such as actinic keratoses, dysplastic nevi,
and advanced solar lentigines, but the repair process takes many months. © 2012 The
International Society of Dermatology.
AN - rayyan-553780587
AU - Heng, M. C. Y.
DO - 10.1111/j.1365-4632.2012.05703.x
IS - 5
KW - Apoptosis
Burns
Curcumin
Enzyme Inhibitors
Gels
Humans
I-kappa B Kinase
NF-kappa B
Phosphorylase Kinase
Signal Transduction
Skin Aging
Skin Diseases
alitretinoin
curcumin
cyclin dependent kinase inhibitor 1
I kappa B kinase alpha
phosphorylase kinase
prednisone
protein kinase B
protein p53
pyrimidine dimer
retinoid
sulfadiazine silver
actinic keratosis
apoptosis
burn
cell proliferation
cell survival
DNA replication
double stranded DNA break
dysplastic nevus
human
lentigo
malignant transformation
photoaging
phototoxicity
point mutation
precancer
protein expression
review
signal transduction
skin carcinogenesis
skin injury
sunburn
ultraviolet A radiation
Skin
PY - 2013
SP - 531-543
ST - Signaling pathways targeted by curcumin in acute and chronic injury: Burns
and photo-damaged skin
T2 - International Journal of Dermatology
TI - Signaling pathways targeted by curcumin in acute and chronic injury: Burns
and photo-damaged skin
84876452159&doi=10.1111%2fj.1365-
4632.2012.05703.x&partnerID=40&md5=d38c3195df9cf5d20653230f0c8baae9
VL - 52
ID - 8614
ER -
TY - JOUR
AB - Purpose: Propolis, a natural honey bee hive product, has anti-inflammatory
and antioxidative properties. We aimed to assess the possible contribution of
topically applied propolis to the suppression of corneal neovascularization (CNV).
Methods: The effect of a water extract of propolis (WEP) 1% drops (group 1) in
comparison with dexamethasone 0.1% (group 2) and saline (group 3) on CNV was tested
in rabbit corneas injured by silver nitrate cauterization. The extent of CNV was
quantitated as the area of CNV and the percent area of CNV for each cornea of the
three groups (12 right eyes per group) in the first week of the treatment. The mean
percent CNV was used for statistical analysis. Results: The corneas treated with
the topical WEP 1% had an almost equal percent CNV as compared with the corneas
treated with topical dexamethasone 0.1% and had less percent CNV than the control
eyes. The quantitative analysis in groups 1, 2 and 3 revealed that the mean percent
CNV was 41.0 +/- 14.1, 39.4 +/- 11.0 and 56.9 +/- 18.4, respectively. The
differences between both groups 1 and 3 as well as groups 2 and 3 were
statistically significant (p = 0.02 and p = 0.01, respectively), whereas the
difference between groups 1 and 2 was not significant (p = 0.86). Conclusions The
topical application of a WEP 1% has an inhibitory effect on CNV in the rabbit's
cornea. The inhibitory effect of propolis was shown to be comparable to that of
topical dexamethasone 0.1%, a potent inhibitor of angiogenesis. We suggest that the
effect of propolis may partially be due to its inhibitory effect on the activity of
both cyclo-oxygenase and lipo-oxygenase.
AN - rayyan-553780588
AU - Hepsen, I. F.
AU - Er, H.
AU - Cekic, O.
DO - 10.1159/000055567
IS - 6
KW - Corneal Neovascularization
Rabbits
PY - 1999
SN - 0030-3747
SP - 426-431
ST - Topically applied water extract of propolis to suppress corneal
neovascularization in rabbits
T2 - OPHTHALMIC RESEARCH
TI - Topically applied water extract of propolis to suppress corneal
neovascularization in rabbits
VL - 31
Y2 - 11
ID - 8615
ER -
TY - JOUR
AB - Background Peroxisome proliferator activated receptor alpha (PPARα), a
regulator of enzymes involved in β oxidation, has been reported to influence
lymphocyte activation. The purpose of this study was to determine whether PPARα
plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). Methods
Wild type (wt) or PPARα-deficient (PPARα−/−) mice were treated with ConA (15 mg/kg)
by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue
collection for analysis of tissue injury, cytokine response, T cell activation and
characterization. Results Ten and 24 h following ConA administration, wt mice had
significant liver injury as demonstrated by serum transaminase levels, inflammatory
cell infiltrate, hepatocyte apoptosis, and expression of several cytokines
including interleukin 4 (IL4) and interferon gamma (IFNγ). In contrast, PPARα−/−
mice were protected from ConA-induced liver injury with significant reductions in
serum enzyme release, greatly reduced inflammatory cell infiltrate, hepatocellular
apoptosis, and IFNγ expression, despite having similar levels of hepatic T cell
activation and IL4 expression. This resistance to liver injury was correlated with
reduced numbers of hepatic natural killer T (NKT) cells and their in vivo
responsiveness to alpha-galactosylceramide. Interestingly, adoptive transfer of
either wt or PPARα−/− splenocytes reconstituted ConA liver injury and cytokine
production in lymphocyte-deficient, severe combined immunodeficient mice
implicating PPARα within the liver, possibly through support of IL15 expression
and/or suppression of IL12 production and not the lymphocyte as the key regulator
of T cell activity and ConA-induced liver injury. Conclusion Taken together, these
data suggest that PPARα within the liver plays an important role in ConA-mediated
liver injury through regulation of NKT cell recruitment and/or survival.
AN - rayyan-553780591
AU - Hines, Ian N.
AU - Kremer, Michael
AU - Moore, Sherri M.
AU - Wheeler, Michael D.
DO - 10.1186/s40659-018-0153-z
KW - Cytokines
Inflammation
Interferon gamma
T helper phenotype
LA - en
PY - 2018
SN - 0716-9760
SP - 5-5
ST - Impaired T cell-mediated hepatitis in peroxisome proliferator activated
receptor alpha (PPARα)-deficient mice
T2 - Biol. Res
TI - Impaired T cell-mediated hepatitis in peroxisome proliferator activated
receptor alpha (PPARα)-deficient mice
97602018000100204
VL - 51
ID - 8618
ER -
TY - JOUR
AB - Background Torsion of the spermatic cord and the resulting testicular
ischemia leads to the production of in-flammatory cytokines and cell death due to
impaired aerobic metabolism. Following reperfusion of the testis, a robust innate
inflammatory response fur-thers tissue injury due to the production of reactive
oxygen species and disruption of normal capillary function. Blunting the innate
immune response with antioxidants, anti-inflammatory medications and targeted
genetic interventions reduces long term testicular injury in animal models of
torsion, how-ever these approaches have limited clinical appli-cability. Mediated
via a7 nACh receptors, the cholinergic anti-inflammatory pathway limits NFKB
signaling and prevents renal fibrosis following warm renal ischemia. We identified
varenicline as an FDA approved a7 nAChR agonist and hypothesized that varenicline
administration would decrease long-term testicular atrophy and fibrosis in a murine
model of testicular torsion. Methods Using an established model, unilateral
testicular torsion was induced in mature male CD1 mice by rotating the right
testicle 720 degrees for 2 h. In the treatment group, 4 doses of varenicline
(1mg/grm)were administered via intraperitoneal injection every 12 h, with the first
dose given 1 h after the creation of testicular torsion. The acute inflamma-tory
response was evaluated 48 h following reper-fusion of the testis. Long term
outcomes were evaluated 30 days following testicular perfusion. Results 48 h
following reperfusion, the testis of animals treated with varenicline demonstrated
a significant reduction in the inflammatory response as measured by the acute
immune cell infiltrate, myeloperox-idase activity, concentration of reduced
glutathione and expression of downstream NF-KB targets. 30 days following
reperfusion, animals treated with varenicline, demonstrated decreased testicular
at-rophy (Summary Figure), fibrosis and expression of pro-fibrotic genes.
Conclusion Activation of a central immunosuppressive cascade with varenicline after
the onset of testicular torsion reduces ischemia reperfusion injury and prevents
long term testicular atrophy and fibrosis. Further studies are needed to define the
optimum dose and varenicline administration regimen. Our results suggest that
varenicline offers a novel, FDA approved, adjunct to the current management of
testicular torsion.
AN - rayyan-553780593
AU - Ho, C.
AU - Zee, R. S.
AU - Omidi, N.
AU - Bayne, C.
AU - Hester, A.
AU - Mukherjee, E.
AU - Sims-Lucas, S.
AU - Mbanefo, E. C.
AU - Hsieh, M. H.
AU - Casella, D. P.
DO - 10.1016/j.jpurol.2021.07.004
IS - 5
KW - Reperfusion Injury
Mice
PY - 2021
SN - 1477-5131 1873-4898
ST - Varenicline limits ischemia reperfusion injury following testicular torsion
in mice
T2 - JOURNAL OF PEDIATRIC UROLOGY
TI - Varenicline limits ischemia reperfusion injury following testicular torsion
in mice
VL - 17
Y2 - 10
ID - 8620
ER -
TY - JOUR
AB - Gold (Au) is long considered as a biocompatible metal, and gold nanoparticles
(AuNPs ∼5 nm) were recently reported to scavenge free radicals. The effect of Au
embedded in a polymeric material is less investigated compared with that of silver.
In this study, nanocomposites from polyurethane (PU) and 43.5 or 174 ppm of AuNPs
were prepared from a waterborne process. The response of endothelial cells (ECs) to
the PU-Au nanocomposites was investigated in vitro and in vivo. ECs on PU-Au
nanocomposites showed lamellipodia formation and better cell proliferation. The
activation of proteins in ECs grown on PU-Au nanocomposites was analyzed by two-
dimensional gel electrophoresis and confirmed by Western blot. The new protein
identified through this procedure was valosin-containing protein (VCP), which is
known to have immunomodulating effect. VCP was upregulated by PU-Au 43.5 ppm and
PU-Au 174 ppm, but more in PU-Au 43.5 ppm. This suggested that the dispersion of
AuNPs in the polymer matrix may be more important than the loading amount. PU-Au
catheters implanted in rat blood vessels showed less foreign body reaction and more
extensive EC coverage than the control PU catheters. The good in vivo
biocompatibility of PU-Au may be associated with the antiinflammatory effect of PU-
Au. Based on this study, AuNPs may serve as an antioxidant additive for biomedical
polymers. © The Author(s) 2012. This article is published with open access at
Springerlink.com.
AN - rayyan-553780594
AU - Ho, T. T.
AU - Lin, Y. C.
AU - Hsu, S. H.
DO - 10.1007/s13404-012-0062-9
IS - 3
Gold nanoparticles (AuNPs)
Polymer nanocomposites
Two-dimensional gel electrophoresis
Additives
Biocompatibility
Blood vessels
Catheters
Cell proliferation
Electrophoresis
Endothelial cells
Free radicals
Functional polymers
Gold
Loading
Metal nanoparticles
Polyurethanes
Proteins
Rat control
Two dimensional
Anti-inflammatories
Anti-inflammatory effects
Antioxidant additives
Biocompatible metals
Biomedical polymers
Foreign body reactions
Gold nanoparticle
Gold Nanoparticles
Human endothelial cells
In-vitro
In-vivo
Lamellipodia
Loading amount
Polymer nanocomposite
Western blots
Nanocomposites
Humanities
Humanism
Humans
PY - 2012
SP - 161-170
ST - Human endothelial cell response to polyurethane-gold nanocomposites
T2 - Gold Bulletin
TI - Human endothelial cell response to polyurethane-gold nanocomposites
84868155542&doi=10.1007%2fs13404-012-0062-
9&partnerID=40&md5=fb245b5f682a55a124f9fe1263f32360
VL - 45
ID - 8621
ER -
TY - JOUR
AB - Obesity leads to the appearance of an inflammatory process, which can be
initiated even with a moderate weight gain. Palmitoylethanolamide (PEA) is an
endogenous lipid, secreted by human adipocytes, that possesses numerous anti-
inflammatory properties. The main purpose of this study was to investigate the
anti-inflammatory effect of PEA on human adipocytes, as well as in a murine model.
The production of tumor necrosis factor-alpha (TNF-alpha) by lipopolysaccharide
(LPS)-treated human subcutaneous adipocytes in primary culture and CF-1 mice was
investigated by enzyme-linked immunosorbent assay. The effects of PEA on adipocyte
TNF-alpha secretion were explored as well as some suspected PEA anti-inflammatory
pathways: nuclear factor-kappaB (NF-kappaB) pathway, peroxisome proliferator-
activated receptor-alpha (PPAR-alpha) gene expression, and TNF-alpha-converting
enzyme (TACE) activity. The effects of PEA on the TNF-alpha serum concentration in
intraperitoneally LPS-treated mice were also studied. We demonstrate that the LPS
induced secretion of TNF-alpha by human adipocytes is inhibited by PEA. This action
is neither linked to a reduction in TNF-alpha gene transcription nor to the
inhibition of TACE activity. Moreover, PPAR-alpha is not implicated in this anti-
inflammatory activity. Lastly, PEA exhibits a wide-reaching anti-inflammatory
action as the molecule is able to completely inhibit the strong increase in TNF-
alpha levels in the serum of mice treated with high doses of LPS. In view of its
virtual lack of toxicity, PEA might become a potentially interesting candidate
molecule in the prevention of obesity-associated insulin resistance.
AN - rayyan-553781930
AU - Hoareau, L.
AU - Buyse, M.
AU - Festy, F.
AU - Ravanan, P.
AU - Gonthier, M. P.
AU - Matias, I.
AU - Petrosino, S.
AU - Tallet, F.
AU - d'Hellencourt, C. L.
AU - Cesari, M.
AU - Di Marzo, V.
AU - Roche, R.
DO - 10.1038/oby.2008.591
IS - 3
KW - ADAM Proteins/metabolism
ADAM17 Protein
Adipocytes/*drug effects/*metabolism
Adult
Amides
Animals
Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
Cells, Cultured
Endocannabinoids
Ethanolamines
Female
Humans
Male
Mice
Mice, Inbred Strains
Middle Aged
Models, Animal
NF-kappa B/metabolism
PPAR alpha/metabolism
Palmitic Acids/*pharmacology
Tumor Necrosis Factor-alpha/*metabolism
Humanities
Humanism
Adipocytes
LA - eng
N1 - Laboratoire de Biochimie et Génétique Moléculaire, Université de La Réunion,
Saint-Denis, France.
PY - 2009
SN - 1930-7381 (Print)
SP - 431-8
ST - Anti-inflammatory effect of palmitoylethanolamide on human adipocytes
TI - Anti-inflammatory effect of palmitoylethanolamide on human adipocytes
VL - 17
Y2 - 3
ID - 9850
ER -
TY - JOUR
AB - The success of total joint replacements has led to consistent growth in the
use of arthroplasty in progressively younger patients. However, more than 10
percent of patients require revision surgeries due to implant failure caused by
osteolytic loosening. These failures are classified as either aseptic or septic and
are associated with the presence of particulate wear debris generated by mechanical
action between implant components. Aseptic loosening results from chronic
inflammation caused by activation of resident immune cells in contact with implant
wear debris. In contrast, septic loosening is defined by the presence of chronic
infection at the implant site. However, recent findings suggest that subclinical
biofilms may be overlooked when evaluating the cause of implant failure, leading to
a misdiagnosis of aseptic loosening. Many of the inflammatory pathways contributing
to periprosthetic joint infections are also involved in bone remodeling and
resorption. In particular, wear debris is increasingly implicated in the inhibition
of the innate and adaptive immune response to resolve an infection or prevent
hematogenous spread. This review examines the interconnectivity of wear particle-
and infection-associated mechanisms of implant loosening, as well as biomaterials-
based strategies to combat infection-related osteolysis.
AN - rayyan-553782390
AU - Hodges, N. A.
AU - Sussman, E. M.
AU - Stegemann, J. P.
DO - 10.1016/j.biomaterials.2021.121127
J2 - Biomaterials
Humans
Inflammation/etiology
*Osteolysis/etiology
LA - eng
N1 - University of Michigan, Department of Biomedical Engineering, Ann Arbor, MI,
48109, USA; Office of Science and Engineering Laboratories, Center for Devices and
Radiological Health, FDA, Silver Spring, MD, 20993, USA. Electronic address:
Nicholas.Hodges@fda.hhs.gov.; Office of Science and Engineering Laboratories,
Center for Devices and Radiological Health, FDA, Silver Spring, MD, 20993, USA.
Electronic address: Eric.Sussman@fda.hhs.gov.; University of Michigan, Department
of Biomedical Engineering, Ann Arbor, MI, 48109, USA. Electronic address:
jpsteg@umich.edu.
PY - 2021
SP - 121127
ST - Aseptic and septic prosthetic joint loosening: Impact of biomaterial wear on
immune cell function, inflammation, and infection
T2 - Biomaterials
TI - Aseptic and septic prosthetic joint loosening: Impact of biomaterial wear on
immune cell function, inflammation, and infection
VL - 278
Y2 - 11
ID - 10297
ER -
TY - JOUR
AB - The inhalation or application of nanoparticles (NPs) has serious impacts on
immunological reactivity. However, the effects of NPs on the immune system are
influenced by numerous factors, which cause a high variability in the results.
Here, mice were exposed to a three month continuous inhalation of copper oxide
(CuO) NPs, and at different time intervals (3, 14, 42 and 93 days), the composition
of cell populations of innate and adaptive immunity was evaluated in the spleen by
flow cytometry. The ability of spleen cells from exposed and control mice to
respond to stimulation with T- or B-cell mitogens by proliferation and by
production of cytokines IL-2, IL-6, IL-10, IL-17 and IFN-gamma was characterized.
The results showed that the inhalation of CuO NPs predominantly affects the cells
of innate immunity (changes in the proportion of eosinophils, neutrophils,
macrophages and antigen-presenting cells) with a minimal effect on the percentage
of T and B lymphocytes. However, the proliferative and secretory activity of T
cells was already significantly enhanced after 3 days from the start of inhalation,
decreased on day 14 and normalized at the later time intervals. There was no
correlation between the impacts of NPs on the cells of innate and adaptive
immunity. The results have shown that the inhalation of CuO NPs significantly
alters the composition of cell populations of innate immunity and modulates the
proliferation and production of cytokines by cells of the adaptive immune system.
However, the immunomodulatory effects of inhaled NPs strongly depend on the time of
inhalation.
AN - rayyan-553780595
AU - Holan, V.
AU - Javorkova, E.
AU - Vrbova, K.
AU - Vecera, Z.
AU - Mikuska, P.
AU - Coufalik, P.
AU - Kulich, P.
AU - Skoupy, R.
AU - Machala, M.
AU - Zajicova, A.
AU - Rossner, P.
DO - 10.1080/17435390.2019.1602679
IS - 7
PY - 2019
SN - 1743-5390 1743-5404
SP - 952-963
ST - A murine model of the effects of inhaled CuO nanoparticles on cells of innate
and adaptive immunity - a kinetic study of a continuous three-month exposure
T2 - NANOTOXICOLOGY
TI - A murine model of the effects of inhaled CuO nanoparticles on cells of innate
and adaptive immunity - a kinetic study of a continuous three-month exposure
VL - 13
Y2 - 8 y3 - 9
ID - 8622
ER -
TY - JOUR
AB - This review highlights and discusses the impact of nanotechnology on the
inhibition of microbial colonization and biofilm development on modified surface
prosthetic devices. In the first part of the paper the current status of infections
related to prosthetic devices and the inquiries resulting from the increased number
of patients with these infections are briefly reviewed. Next we discuss several
aspects about the implication of nanotechnology in prosthetic devices surface
modification and its impact on the prevention of infections. The main aspects
regarding the biocompatibility and the application of these nanomodified prosthetic
devices in tissue engineering are also highlighted. © 2015 Bentham Science
Publishers
AN - rayyan-553780596
AU - Holban, A. M.
AU - Iordanskii, A.
AU - Bychkova, A.
AU - Andronescu, E.
AU - Mogoantă, L. I.
AU - Mogo Ș Anu, G. D.
AU - Iordache, F.
DO - 10.2174/138920101602150112150303
IS - 2
KW - Antimicrobial coating
Biocompatible nanoparticles
Biofilm inhibition
Modified surface
Prosthetic device
Animals
Bacterial Infections
Biofilms
Humans
Mycoses
Nanostructures
Nanotechnology
Surface Properties
alginic acid
alloy
aluminum oxide
cefotaxime
cellulose
chitin
collagen
erythromycin
hydroxyapatite
interleukin 12
kanamycin
metal oxide
molecular scaffold
nanomaterial
poly (methyl methacrylate)
polycaprolactone
polyglactin
polylactide
polymyxin B
polypropylene
polyvinyl alcohol
polyvinylchloride
silver nanoparticle
streptomycin
titanium dioxide
unclassified drug
zirconium oxide
biomaterial
Article
bacterial growth
bacterium contamination
biocompatibility
biodegradable implant
biofilm
bone remodeling
cell proliferation
ceramic prosthesis
clinical trial (topic)
growth inhibition
human
infection prevention
inflammatory infiltrate
material coating
nanotechnology
nonhuman
prosthesis infection
surface property
tissue engineering
tissue regeneration
animal
growth, development and aging
prostheses and orthoses
Colon
PY - 2015
SP - 112-120
ST - Prosthetic devices with nanostructurated surfaces for increased resistance to
T2 - Current Pharmaceutical Biotechnology
TI - Prosthetic devices with nanostructurated surfaces for increased resistance to
84926151693&doi=10.2174%2f138920101602150112150303&partnerID=40&md5=22d3da349bcff86
0a938e14404f75301
VL - 16
ID - 8623
ER -
TY - JOUR
AB - Background: The uses of engineered nanomaterials have expanded in biomedical
technology and consumer manufacturing. Furthermore, pulmonary exposure to various
engineered nanomaterials has, likewise, demonstrated the ability to exacerbate
cardiac ischemia reperfusion (I/R) injury. However, the influence of particle size
or capping agent remains unclear. In an effort to address these influences we
explored response to 2 different size gold core nanosilver particles (AgNP) with
two different capping agents at 2 different time points. We hypothesized that a
pulmonary exposure to AgNP induces cardiovascular toxicity influenced by
inflammation and vascular dysfunction resulting in expansion of cardiac I/R Injury
that is sensitive to particle size and the capping agent. Methods: Male Sprague-
Dawley rats were exposed to 200 μg of 20 or 110 nm polyvinylprryolidone (PVP) or
citrate capped AgNP. One and 7 days following intratracheal instillation serum was
analyzed for concentrations of selected cytokines; cardiac I/R injury and isolated
coronary artery and aorta segment were assessed for constrictor responses and
endothelial dependent relaxation and endothelial independent nitric oxide dependent
relaxation. Results: AgNP instillation resulted in modest increase in selected
serum cytokines with elevations in IL-2, IL-18, and IL-6. Instillation resulted in
a derangement of vascular responses to constrictors serotonin or phenylephrine, as
well as endothelial dependent relaxations with acetylcholine or endothelial
independent relaxations by sodium nitroprusside in a capping and size dependent
manner. Exposure to both 20 and 110 nm AgNP resulted in exacerbation cardiac I/R
injury 1 day following IT instillation independent of capping agent with 20 nm AgNP
inducing marginally greater injury. Seven days following IT instillation the
expansion of I/R injury persisted but the greatest injury was associated with
exposure to 110 nm PVP capped AgNP resulted in nearly a two-fold larger infarct
size compared to na�ve. Conclusions: Exposure to AgNP may result in vascular
dysfunction, a potentially maladaptive sensitization of the immune system to
respond to a secondary insult (e.g., cardiac I/R) which may drive expansion of I/R
injury at 1 and 7 days following IT instillation where the extent of injury could
be correlated with capping agents and AgNP size. � 2016 The Author(s).
AN - rayyan-553780598
AU - Holland, N. A.
AU - Thompson, L. C.
AU - Vidanapathirana, A. K.
AU - Urankar, R. N.
AU - Lust, R. M.
AU - Fennell, T. R.
AU - Wingard, C. J.
DO - 10.1186/S12989-016-0159-Z
IS - 1
KW - Aorta
Coronary artery
Myocardial infarction
Nanotoxicology
Pulmonary instillation
Serum cytokines
Animals
Gold
Heart Injuries
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Particle Size
Rats
Silver
gamma interferon
granulocyte colony stimulating factor
interleukin 10
interleukin 13
interleukin 18
interleukin 1beta
interleukin 2
interleukin 5
interleukin 6
RANTES
silver nanoparticle
gold
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
cardiotoxicity
controlled study
heart muscle ischemia
in vivo study
male
nonhuman
particle size
priority journal
protein blood level
protein determination
rat
zeta potential
animal
chemically induced
chemistry
exposure
heart injury
lung
Sprague Dawley rat
Cardiovascular Agents
PY - 2016
ST - Impact of pulmonary exposure to gold core silver nanoparticles of different
size and capping agents on cardiovascular injury
TI - Impact of pulmonary exposure to gold core silver nanoparticles of different
size and capping agents on cardiovascular injury
85007489612&doi=10.1186%2fS12989-016-0159-
Z&partnerID=40&md5=2c141fd65191416baa2fb3f98a3b4dea
VL - 13
ID - 8625
ER -
TY - JOUR
AB - Although TiO2 nanopartides (NPs) as endocrine disruptors have been
demonstrated to be able to cross the blood testis barriers and induce reproductive
toxicity in male animals, whether the reproductive toxicity of male animals due to
exposure to endocrine disruptor TiO2 NPs is related to immunological dysfunction in
the testis remains not well understood. This study determined whether the
reproductive toxicity and immunological dysfunction induced by exposure to TiO2 NPs
is associated with activation or inhibition of TAM/TLR-mediated signal pathway in
mouse testis. The results showed that male mice exhibited significant reduction of
fertility, infiltration of inflammatory cells, rarefaction, apoptosis, and/or
necrosis of spermatogenic cells and Sertoli cells due to TiO2 NPs. Furthermore,
these were associated with decreased expression of Tyro3 (-18.16 to -66.6%), Axl (-
14.7 to -57.99%), Mer (-7.98 to -72.62%), and I kappa B (-11.25 to -63.16%),
suppression of cytokine signaling (SOCS) 1 (-21.99 to -73.8%) and SOCS3 (-8.11 to -
34.86%), and increased expression of Toll-like receptor (TLR)-3 (21.4-156.03%),
TLR-4 (37.0-109.87%), nuclear factor-kappa B (14.75-69.34%), interleukin (1L)-1
beta (46.15-123.08%), IL-6 (2.54-81.98%), tumor necrosis factor-alpha (6.95-
88.39%), interferon (IFN)-alpha (2.54-37.25%), and IFN-beta (10.19-80.56%), which
are involved in the immune environment in the testis. The findings showed that
reproductive toxicity of male mice induced by exposure to endocrine disruptor TiO2
NPs may be associated with biomarkers of impairment of immune environment or
dysfunction of TAM/TLR3-mediated signal pathway in mouse testitis. Therefore, the
potential risks to reproductive health should be attended, especially in those who
are occupationally exposed to TiO2 NPs.
AN - rayyan-553780600
AU - Hong, F. S.
AU - Wang, Y. J.
AU - Zhou, Y. J.
AU - Zhang, Q.
AU - Ge, Y. S.
AU - Chen, M.
AU - Hong, J.
AU - Wang, L.
DO - 10.1021/acs.jafc.5b05262
IS - 1
KW - Mice
PY - 2016
SN - 0021-8561 1520-5118
SP - 346-355
ST - Exposure to TiO2 Nanoparticles Induces Immunological Dysfunction in Mouse
Testitis
T2 - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
TI - Exposure to TiO2 Nanoparticles Induces Immunological Dysfunction in Mouse
Testitis
VL - 64
Y2 - 1 y3 - 13
ID - 8627
ER -
TY - JOUR
AB - Infectious diseases caused by new or unknown bacteria and viruses, such as
anthrax, cholera, tuberculosis and even COVID-19, are a major threat to humanity.
Thus, the development of new synthetic compounds with efficient antimicrobial
activity is a necessity. Herein, rationally designed novel multifunctional cationic
alternating copolymers were directly synthesized through a step-growth
polymerization reaction using a bivalent electrophilic cross-linker containing
disulfide bonds and a diamine heterocyclic ring. To optimize the activity of these
alternating copolymers, several different diamines and cross-linkers were explored
to find the highest antibacterial effects. The synthesized nanopolymers not only
displayed good to excellent antibacterial activity as judged by minimum inhibitory
concentration (MIC) and minimum bactericidal concentration (MBC) against
Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and
Escherichia coli, but also reduced the number of biofilm cells even at low
concentrations, without killing mammalian cells. Furthermore, in vivo experiments
using infected burn wounds in mice demonstrated good antibacterial activity and
stimulated wound healing, without causing systemic inflammation. These findings
suggest that the multifunctional cationic nanopolymers have potential as a novel
antibacterial agent for eradication of multidrug resistant bacterial infections. ©
2021
AN - rayyan-553780603
AU - Hooshmand, S. E.
AU - Ebadati, A.
AU - Hosseini, E. S.
AU - Vahabi, A. H.
AU - Oshaghi, M.
AU - Rahighi, R.
AU - Orooji, Y.
AU - Jahromi, M. A. M.
AU - Varma, R. S.
AU - Hamblin, M. R.
AU - Karimi, M.
DO - 10.1016/j.bioorg.2021.105550
KW - Alternating copolymer
Antibacterial activity
Antibiofilm activity
Cationic polymer
Nanomedicine
Wound healing
Amines
Animals
Bacteria
Biofilms
Burns
Cations
Cell Survival
COVID-19
Cross-Linking Reagents
Drug Resistance, Multiple, Bacterial
HEK293 Cells
Humans
Mice
Polymers
Wound Healing
1,4 diazabicyclo[2.2.2]octane
antibiotic agent
cation
cystamine
gentamicin
gold nanoparticle
graphene oxide
imidazole
interleukin 1
interleukin 6
ketamine
metal organic framework
metal organic framework 199
methenamine
piperazine
polymer nanoparticle
silver nanoparticle
spermine
unclassified drug
xylazine
amine
antiinfective agent
cross linking reagent
polymer
animal experiment
animal model
Article
bacterial strain
Bagg albino mouse
burn infection
cell regeneration
cell viability
colorimetry
comparative study
controlled study
cross linking
cytotoxicity
cytotoxicity assay
disulfide bond
drug design
drug potency
drug synthesis
electrophilicity
Enterococcus faecalis
Escherichia coli
gel permeation chromatography
HEK293 cell line
hospital infection
human
human cell
in vitro study
in vivo study
mouse
MTT assay
nanoanalysis
nonhuman
nuclear magnetic resonance
particle size
polymerization
protein blood level
reaction time
wound healing
zeta potential
animal
bacterial infection
bacterium
biofilm
burn
cell survival
chemistry
complication
drug effect
PY - 2022
ST - Antibacterial, antibiofilm, anti-inflammatory, and wound healing effects of
nanoscale multifunctional cationic alternating copolymers
T2 - Bioorganic Chemistry
TI - Antibacterial, antibiofilm, anti-inflammatory, and wound healing effects of
nanoscale multifunctional cationic alternating copolymers
85121271231&doi=10.1016%2fj.bioorg.2021.105550&partnerID=40&md5=1a333915523d12e042d
9c1090295c84b
VL - 119
ID - 8630
ER -
TY - JOUR
AB - Impaired wound healing is a common complication associated with diabetes with
complex pathophysiological underlying mechanisms and often necessitates amputation.
With the advancement in laser technology, irradiation of these wounds with low-
intensity laser irradiation (LILI) or phototherapy, has shown a vast improvement in
wound healing. At the correct laser parameters, LILI has shown to increase
migration, viability, and proliferation of diabetic cells in vitro; there is a
stimulatory effect on the mitochondria with a resulting increase in adenosine
triphosphate (ATP). In addition, LILI also has an anti-inflammatory and protective
effect on these cells. In light of the ever present threat of diabetic foot ulcers,
infection, and amputation, new improved therapies and the fortification of wound
healing research deserves better prioritization. In this review we look at the
complications associated with diabetic wound healing and the effect of laser
irradiation both in vitro and in vivo in diabetic wound healing. © 2014 Nicolette
N. Houreld.
AN - rayyan-553780605
AU - Houreld, N. N.
DO - 10.1155/2014/398412
KW - Animals
Diabetes Complications
Humans
Laser Therapy, Low-Level
Phototherapy
Treatment Outcome
Wound Healing
Wounds and Injuries
antibiotic agent
collagen
cyclic AMP
cytochrome c oxidase
nitric oxide
procollagen
sulfadiazine silver
vasculotropin
aerobic metabolism
antibiotic therapy
apoptosis
calcium cell level
cell death
cell migration
cell proliferation
cell viability
collagen synthesis
debridement
diabetes mellitus
diabetic foot
human
hyperbaric oxygen
hyperglycemia
hypoxemia
in vitro study
in vivo study
leg revascularization
light emitting diode
low level laser therapy
nonhuman
oxidative stress
pathogenesis
phototherapy
randomized controlled trial (topic)
review
upregulation
wound infection
animal
procedures
treatment outcome
wound healing
PY - 2014
ST - Shedding light on a new treatment for diabetic wound healing: A review on
phototherapy
T2 - The Scientific World Journal
TI - Shedding light on a new treatment for diabetic wound healing: A review on
phototherapy
84893166567&doi=10.1155%2f2014%2f398412&partnerID=40&md5=ce7abf9c3f32e7bd9702211114
e72836
VL - 2014
ID - 8632
ER -
TY - JOUR
AB - A novel injectable hydrogel dressing (GA@AgNPs-SA) with long-term
antimicrobial effect is developed that can accelerate the closure of bacteria-
infected wounds. The hydrogel dressing was prepared by cross-linking sodium
alginate molecular chains and gallic acid functionalized silver nanoparticles
(GA@AgNPs) via calcium ions to form a three-dimensional network. The hydrogel
dressing demonstrates excellent biocompatibility and can achieve a sustainable
release of silver ions, ensuring a long-term antibacterial activity and inhibiting
biofilm formation. Moreover, an in vivo study demonstrates that the GA@AgNPs-SA
hydrogel can effectively decrease the expression of IL-6 and TNF-α to alleviate the
inflammatory response, and promote angiogenesis by upregulating CD31, α-SMA and
VEGF expression, thus significantly accelerating the repair of infected wounds.
Given these interesting properties, this antibacterial hydrogel has great potential
for application in the clinical care of bacteria-infected wounds. © 2023
AN - rayyan-553780607
AU - Hu, Q.
AU - Nie, Y.
AU - Xiang, J.
AU - Xie, J.
AU - Si, H.
AU - Li, D.
AU - Zhang, S.
AU - Li, M.
AU - Huang, S.
DO - 10.1016/j.ijbiomac.2023.123691
KW - Injectable hydrogel
Plant polyphenol-functionalized silver nanoparticles
Wound healing
Alginates
Hydrogels
Ions
Metal Nanoparticles
Silver
Wound Healing
alginic acid
alpha smooth muscle actin
calcium ion
gallic acid
hydrogel
interleukin 6
polyphenol
silver nanoparticle
silver nitrate
vasculotropin
antiinfective agent
ion
metal nanoparticle
silver
angiogenesis
animal experiment
animal tissue
Article
bacterial infection
bactericidal activity
biocompatibility
biofilm
cell viability
Escherichia coli
inflammation
male
nonhuman
wound healing
wound infection
zone of inhibition
pharmacology
PY - 2023
ST - Injectable sodium alginate hydrogel loaded with plant polyphenol-
functionalized silver nanoparticles for bacteria-infected wound healing
TI - Injectable sodium alginate hydrogel loaded with plant polyphenol-
functionalized silver nanoparticles for bacteria-infected wound healing
85148373889&doi=10.1016%2fj.ijbiomac.2023.123691&partnerID=40&md5=eeca901c5d1f34af1
0a0a574b1ff9fb8
VL - 234
ID - 8634
ER -
TY - JOUR
AB - With the rapid development of nanotechnology, there is a growing interest on
the application of nanoparticles in various fields such as photonics, catalysis,
magnetics, and biotechnology including cosmetics, pharmaceutics, and medicines.
However, little is known about their potential toxicity to human health. Owing to
their special properties, nanoparticles have the capacity to bypass the blood-brain
barrier (BBB). However, the toxic effects of nanoparticles on central nervous
system (CNS) function are still lacking. And the interactions of nanoparticles with
the cells and tissues in CNS are poorly understood. Thus, neurotoxicity induced by
nanoparticles is still a new topic that requires more attention. In this review, we
summarized the pathways by which the nanoparticles could enter into the CNS and the
recent investigations on the neurotoxicity of nanoparticles both in vitro and in
vivo, as well as the potential mechanisms. Furthermore, the future direction in the
neurotoxicity studies of nanoparticles is also discussed. © 2010 Elsevier B.V.
AN - rayyan-553780608
AU - Hu, Y. L.
AU - Gao, J. Q.
DO - 10.1016/j.ijpharm.2010.04.026
IS - 1
KW - Nanoparticles
Neurotoxicity
Polymer nanocarrier
Animals
Blood-Brain Barrier
Brain
Humans
Nanotechnology
copper nanoparticle
CXCL9 chemokine
dihydroxyphenylacetic acid
dopamine
doxorubicin
ferric oxide nanoparticle
fullerene
homovanillic acid
interleukin 1beta
leucine enkephalin[2 dextro alanine 6 arginine]
metal nanoparticle
nanoparticle
nerve cell adhesion molecule
polysorbate 80
silver nanoparticle
titanium oxide nanoparticle
transcription factor AP 1
ultrafine carbon black nanoparticle
unclassified drug
blood brain barrier
cell line
cell viability
central nervous system function
cytotoxicity
drug penetration
drug solubility
drug stability
drug targeting
glioblastoma
human
in vitro study
in vivo study
lipid peroxidation
microarray analysis
nerve cell lesion
neurotoxicity
nonhuman
oxidative stress
particle size
physical chemistry
priority journal
review
toxicokinetics
animal
brain
metabolism
nanotechnology
procedures
toxicity
PY - 2010
SP - 115-121
ST - Potential neurotoxicity of nanoparticles
T2 - International Journal of Pharmaceutics
TI - Potential neurotoxicity of nanoparticles
77953717599&doi=10.1016%2fj.ijpharm.2010.04.026&partnerID=40&md5=2284af8e982ff148b5
e928ce6c80b0b4
VL - 394
ID - 8635
ER -
TY - JOUR
AB - Although apoptotic cellular degeneration has been reported to be extremely
rapid with the use of in vitro models, the time needed to clear apoptotic neurons
in the in vivo brain is unknown. In this study we used a simple morphological
approach to solve this problem. Four days after adrenalectomy (ADX), all of the
operated rats morphologically displayed hippocampal granule cell apoptosis that was
prevented completely by corticosterone replacement immediately after ADX.
Therefore, we intravenously injected the rats with corticosterone 4 d after ADX and
subsequently maintained them on corticosterone replacement in saline drinking
water. This corticosterone replacement could protect healthy granule cells promptly
and continuously against hormone-deficient apoptosis, because the normal
glucocorticoid receptor immunoreactivity within the granule cell nuclei, which
disappeared after ADX, was identified 1 hr after corticosterone replacement was
started, and this effect persisted for several days. However, this corticosterone
treatment could not prevent the irreversible apoptosis of the already degenerated
granule cells at various stages of the same progressive apoptotic process. Then we
successively traced the disappearance of apoptotic granule cells throughout the
hippocampus at different time points by Nissl and silver staining. Given that the
apoptotic cells at the earliest stage of the degenerating process when the ADX rats
received corticosterone injection were the last to disappear, the period from
corticosterone injection until the disappearance of the last degenerating debris of
apoptotic cells was taken to represent the time course for elimination of apoptotic
neurons in vivo. We discovered that the elimination of apoptotic granule cells took
72 hr.
AN - rayyan-553782215
AU - Hu, Z.
AU - Yuri, K.
AU - Ozawa, H.
AU - Lu, H.
AU - Kawata, M.
DO - 10.1523/JNEUROSCI.17-11-03981.1997
IS - 11
J2 - J Neurosci
KW - *Adrenalectomy
Animals
Antibody Specificity
Apoptosis/drug effects/*physiology
Chromatin/pathology
Corticosterone/pharmacology
Hippocampus/chemistry/pathology/*physiology
Male
Nissl Bodies/chemistry
Rats
Receptors, Glucocorticoid/analysis/immunology
Silver Staining
Time Factors
Adrenalectomy
Hippocampus
LA - eng
N1 - Department of Anatomy and Neurobiology, Kyoto Prefectural University of
Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan.
PY - 1997
SN - 0270-6474 (Print)
SP - 3981-9
ST - The in vivo time course for elimination of adrenalectomy-induced apoptotic
profiles from the granule cell layer of the rat hippocampus
T2 - The Journal of neuroscience : the official journal of the Society for
Neuroscience
TI - The in vivo time course for elimination of adrenalectomy-induced apoptotic
profiles from the granule cell layer of the rat hippocampus
VL - 17
Y2 - 6 y3 - 1
ID - 10125
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have antibacterial characteristics, and
currently are applied in Ag-containing products. This study found neural cells can
uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene
expression of inflammation and neurodegenerative disorder in murine brain ALT
astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5.
μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and
induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor
with collagenous structure (MARCO) and glutathione synthetase (GSS) for
inflammatory response and oxidative stress neutralization. Additionally, this study
found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD)
deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene
expression of amyloid precursor protein (APP) was induced, and otherwise,
neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural
cells as well as protein level. These results suggested AgNPs could alter gene and
protein expressions of Aβ deposition potentially to induce AD progress in neural
cells. It's necessary to take notice of AgNPs distribution in the environment. ©
2014 The Authors.
AN - rayyan-553780609
AU - Huang, C. L.
AU - Hsiao, I. L.
AU - Lin, H. C.
AU - Wang, C. F.
AU - Huang, Y. J.
AU - Chuang, C. Y.
DO - 10.1016/j.envres.2014.11.006
KW - Alzheimer 's disease
Gene expression
Inflammation
Neurodegenerative disorder
Silver nanoparticle
Animals
Brain
Gene Expression
Metal Nanoparticles
Mice
Silver
CXCL13 chemokine
glutathione synthase
interleukin 1beta
low density lipoprotein receptor
membrane metalloendopeptidase
silver nanoparticle
metal nanoparticle
silver
brain
gene expression
neurology
oxidation
particle size
physiological response
protein
Alzheimer disease
animal cell
Article
astrocyte
brain nerve cell
cell proliferation
controlled study
cytokine production
cytotoxicity
inflammation
microglia
mouse
nerve degeneration
nonhuman
oxidative stress
protein induction
Western blotting
animal
chemistry
cytology
drug effects
genetics
Nanoparticles
PY - 2015
SP - 253-263
ST - Silver nanoparticles affect on gene expression of inflammatory and
neurodegenerative responses in mouse brain neural cells
T2 - Environmental Research
TI - Silver nanoparticles affect on gene expression of inflammatory and
neurodegenerative responses in mouse brain neural cells
84911365647&doi=10.1016%2fj.envres.2014.11.006&partnerID=40&md5=5c907ed34e1d9134799
3c2e60b678b83
VL - 136
ID - 8636
ER -
TY - JOUR
AB - Histologic investigations have demonstrated that root canal sealers can
induce mild to severe inflammatory alternations. However, there is little
information on the precise mechanisms about root canal sealer-induced inflammatory
reaction. The proteolysis of extracellular matrix by matrix metalloproteinases
(MMPs) and plasminogen activators (PAs) seems to be a key initiating event for the
progression of the inflammatory process. The aim of this study was to investigate
the effects of epoxy resin-based root canal sealer AH26 and zinc oxide-eugenol-
based root canal sealer Canals and one paste sealer N2 on the expression of MMPs
and PAs in human osteoblastic cell line U2OS cells. The levels of gelatinolytic and
caseinolytic activities were measured by gelatin and casein zymography. The results
showed that AH26, Canals, and N2 were cytotoxic to U2OS cells in a concentration-
dependent manner (P < .05). The gelatin zymograms revealed that MMP-2 (72 kd) and
MMP-9 (92 kd) were secreted by U2OS cells. The exposure of U2OS cells to root canal
sealers resulted in the up-regulation of MMP-2 and MMP-9 expression (P < .05).
Casein zymography exhibited a caseinolytic band with a molecular weight of 70 kd,
indicative of the presence of tissue type plasminogen activators (t-PA). t-PA was
also found to be up-regulated by root canal sealers (P < .05). Taken together, the
activation of gelatinases and t-PA might play an important role in the pathogenesis
of root canal sealer-induced periapical inflammation.
AN - rayyan-553782189
AU - Huang, F. M.
AU - Yang, S. F.
AU - Chang, Y. C.
DO - 10.1016/j.joen.2007.12.021
IS - 3
J2 - J Endod
KW - Bismuth/toxicity
Cells, Cultured
Drug Combinations
Electrophoresis, Polyacrylamide Gel
Eugenol/toxicity
Formaldehyde/toxicity
Humans
Matrix Metalloproteinase 2/*biosynthesis
Matrix Metalloproteinase 9/*biosynthesis
Osteoblasts/drug effects/*enzymology
Silver/toxicity
Tissue Plasminogen Activator/*biosynthesis
Titanium/toxicity
Up-Regulation
Zinc Oxide/toxicity
Zinc Oxide-Eugenol Cement/toxicity
Humanities
Humanism
Tissue Plasminogen Activator
LA - eng
N1 - Oral Medicine Center, Chung Shan Medical University Hospital, Taichung,
Taiwan.
PY - 2008
SN - 0099-2399 (Print)
SP - 291-4
ST - Up-regulation of gelatinases and tissue type plasminogen activator by root
canal sealers in human osteoblastic cells
TI - Up-regulation of gelatinases and tissue type plasminogen activator by root
canal sealers in human osteoblastic cells
VL - 34
Y2 - 3
ID - 10099
ER -
TY - JOUR
AB - Self-assemblies of bioactively natural compounds into supramolecular
hydrogels without structural modifications are of interest to improve their
sustained releases and bioavailabilities in vivo. However, it is still a formidable
challenge to dig out such a naturally small molecule with a meticulous structure
which can be self-assembled to form a hydrogel for biomedical applications. Here, a
new hydrogel consisting only of gallic acid (GA) via π–π stacking and hydrogen bond
interactions, whereas none of GA analogues can form the similar supramolecular
hydrogels, is reported. This interesting phenomenon is intriguing to further
investigate the potential applications of GA hydrogels in wound healing. Notably,
this GA hydrogel has rod-like structures with lengths varying from 10 to 100 µm.
The biocompatibility and antibacterial tests prove that this well-assembled GA
hydrogel has no cytotoxicity and excellent antibacterial activities against
Escherichia coli and Staphylococcus aureus. Moreover, the GA hydrogel can
significantly accelerate the process of wound healing with or without bacterial
infections by mediation of inflammation signaling pathways. It is believed that the
current study may shed a new light on the design of a supramolecular hydrogel based
on self-assemblies of naturally small molecules to improve their bioavailabilities
and diversify their uses in biomedical applications. © 2022 Wiley-VCH GmbH.
AN - rayyan-553780612
AU - Huang, H.
AU - Gong, W.
AU - Wang, X.
AU - He, W.
AU - Hou, Y.
AU - Hu, J.
DO - 10.1002/adhm.202102476
IS - 12
polyphenols
self-assembly
supramolecular hydrogels
wound healing
Escherichia coli
Hydrogels
Wound Healing
Biochemistry
Biocompatibility
Hydrogen bonds
Molecules
Self assembly
Supramolecular chemistry
antiinfective agent
cinnamic acid
claudin 1
ellagic acid
high mobility group B1 protein
interleukin 6
nanofiber
polyvinylidene fluoride
protocatechualdehyde
protocatechuic acid
self assembled nanoparticle
silver nanoparticle
Fibrillar networks
Gallic acids
Natural compounds
Polyphenols
Small molecules
Supramolecular hydrogels
Wound healing
angiogenesis
Article
bacterial viability
bioavailability
biocompatibility
biodegradability
biofilm
cell proliferation
cell viability
chemoluminescence
circular dichroism
colony forming unit
cytotoxicity
drug synthesis
electrochemiluminescence
immunofluorescence
molecular library
MTT assay
protein expression
signal transduction
supramolecular chemistry
tissue regeneration
Western blotting
wound healing assay
zone of inhibition
chemistry
hydrogel
pharmacology
PY - 2022
ST - Self-Assembly of Naturally Small Molecules into Supramolecular Fibrillar
Networks for Wound Healing
T2 - Advanced Healthcare Materials
TI - Self-Assembly of Naturally Small Molecules into Supramolecular Fibrillar
Networks for Wound Healing
85128961876&doi=10.1002%2fadhm.202102476&partnerID=40&md5=3da64a5eb911bad5f18c5f161
cd351fc
VL - 11
ID - 8639
ER -
TY - JOUR
AB - (Arginine-Glycine-Aspartic)-methoxy polyethylene glycol-(1,2-distearoyl-sn-
glycero-3-phosphoethanolaMine-N) (abbreviation: RGD-PEG2000-DSPE or RGD-PD) was
successfully synthesized and verified by H-1-NMR and MALDI-TOF MS. Polyethylene
glycol-poly-L-lysine/RGD-PD/phospholipid/calcium phosphate nanoparticles
(PEG-PLL/RGD-PD/PL/CaP NPs or MNPs) were prepared using a novel, simple method
conducted at room temperature. Transmission electron microscopy (TEM) analysis
showed that the MNPs were spheres of uniform size, with a diameter of similar to 30
nm, and smooth surface. Thermogravimetric analysis (TGA) revealed that the PEG-
PLL/RGD-PD/PL micelle was packed in the CaP shell. MNPs had little effect on
hemolysis, coagulation, cardiac oxidative stress, inflammatory response and DNA
damage, indicating negligible cytotoxicity in vitro and in vivo. Experiments in
Zebrafish indicated that the MNPs neither affected the survival rate and heartbeat
rate, nor induced malformation and apoptosis during embryogenesis. In conclusion,
these results demonstrate that the newly-developed MNPs have good biocompatibility
and a great potential as drug and gene carrier.
AN - rayyan-553780613
AU - Huang, J. W.
AU - Zhang, X. Y.
AU - Wu, Z. H.
AU - Wu, Y. F.
AU - Wu, X. J.
AU - Wang, Y. G.
AU - Jiang, H.
AU - Ma, J.
AU - He, Z. L.
DO - 10.1166/jbn.2018.2460
IS - 1
PY - 2018
SN - 1550-7033 1550-7041
SP - 98-113
ST - Preparation and Biocompatibility Evaluation of
PEG-PLL/RGD-PEG-DSPE/Phospholipid/CaP Nanoparticles
TI - Preparation and Biocompatibility Evaluation of
PEG-PLL/RGD-PEG-DSPE/Phospholipid/CaP Nanoparticles
VL - 14
Y2 - 1
ID - 8640
ER -
TY - JOUR
AB - Xanthan gum is a high molecular weight polysaccharide biocompatible to
biological systems, so its products promise high potential in medicine. In this
study, we crosslinked xanthan gum with citric acid to develop a transparent film
for protecting the wound. Silver nanoparticles (AgNPs) are incorporated into the
film to enhance the antimicrobial property of our biomaterial. This paper discussed
the characteristics and manufacturing of this nanocomposite dressing. The safety of
the dressing was studied using fibroblasts (L929) by the method of 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and staining of
ethidium homodimer (PI) and calcein AM. The bacterial inhibition test and
application of the dressing to nonhealing wounds infected with methicillin-
resistant S. aureus (MRSA) were performed to evaluate the antibacterial effects in
vitro and in vivo, respectively. The results indicated that the dressing could
restrict the formation of biofilms, reduce inflammatory reactions, and promote the
angiogenesis of granulation tissues in infectious wounds. Therefore, this dressing
has a great advantage over traditional clinical products especially when
administered under the condition of infections or for the purpose of infection
prevention. © 2017 Jinjian Huang et al.
AN - rayyan-553780614
AU - Huang, J.
AU - Ren, J.
AU - Chen, G.
AU - Deng, Y.
AU - Wang, G.
AU - Wu, X.
DO - 10.1155/2017/6802397
Cell culture
Metal nanoparticles
Nanoparticles
Xanthan gum
Anti-microbial properties
Antibacterial effects
Bacterial inhibition
Ethidium homodimer
High molecular weight
Inflammatory reaction
Silver
PY - 2017
ST - Evaluation of the Xanthan-Based Film Incorporated with Silver Nanoparticles
for Potential Application in the Nonhealing Infectious Wound
T2 - Journal of Nanomaterials
TI - Evaluation of the Xanthan-Based Film Incorporated with Silver Nanoparticles
for Potential Application in the Nonhealing Infectious Wound
85029804801&doi=10.1155%2f2017%2f6802397&partnerID=40&md5=76cde43d58729b8bb32b91e44
3f7e15c
VL - 2017
ID - 8641
ER -
TY - JOUR
AB - Neutrophil extracellular traps (NETs) stick to bacteria and prevent
infections in vivo, whose activation is upon inflammatory stimuli along with the
sudden increase of reactive oxygen species (ROS). Nevertheless, the risky over
activation in NETosis may result in deleterious outcome. A big challenge in using
NETs for therapeutics is to synthesize an artificial system that can function as
NETs in vivo. Here, we developed an in vivo supramolecular assembly system to
imitate the innate immune process of NETs to inhibit methicillin-resistant
staphylococcus epidermidis (MRSE) infection. Our synthesized small molecules
undergo oxidation to form supramolecular nanofibers at inflammatory loci. The in
situ formed nanofibers network efficiently traps MRSE cells and prevent them from
aggressive dissemination. The extended interactions between nanofibers and bacteria
directly result in the death of MRSE via the transcriptomes alterations. In
clinically relevant models (intraperitoneal infection and catheter implantation),
our supramolecular nets show significant antibacterial activity, yielding a three
times efficacy comparing to vancomycin. The spontaneous consumption of ROS and the
formation of antibacterial networks create a steady negative feedback system to
combat bacterial infections.
AN - rayyan-553780615
AU - Huang, Z. T.
AU - Liu, Y.
AU - Wang, L.
AU - Ali, A.
AU - Yao, Q. X.
AU - Jiang, X. Y.
AU - Gao, Y.
DO - 10.1016/j.biomaterials.2020.120124
KW - Infection Control
PY - 2020
SN - 0142-9612 1878-5905
ST - Supramolecular assemblies mimicking neutrophil extracellular traps for MRSE
infection control
T2 - BIOMATERIALS
TI - Supramolecular assemblies mimicking neutrophil extracellular traps for MRSE
infection control
VL - 253
Y2 - 9
ID - 8642
ER -
TY - JOUR
AB - Background: Nanosilver is one of the most commonly used engineered
nanomaterials (ENMs). In our study we focused on assessing the size-dependence of
the toxicity of nanosilver (Ag ENMs), utilising materials of three sizes (50, 80
and 200 nm) synthesized by the same method, with the same chemical composition,
charge and coating. Methods: Uptake and localisation (by Transmission Electron
Microscopy), cell proliferation (Relative growth activity) and cytotoxic effects
(Plating efficiency), inflammatory response (induction of IL-8 and MCP-1 by Enzyme
linked immune sorbent assay), DNA damage (strand breaks and oxidised DNA lesions by
the Comet assay) were all assessed in human lung carcinoma epithelial cells (A549),
and the mutagenic potential of ENMs (Mammalian hprt gene mutation test) was
assessed in V79-4 cells as per the OECD protocol. Detailed physico-chemical
characterization of the ENMs was performed in water and in biological media as a
prerequisite to assessment of their impacts on cells. To study the relationship
between the surface area of the ENMs and the number of ENMs with the biological
response observed, Ag ENMs concentrations were recalculated from mu g/cm(2) to ENMs
cm(2)/cm(2) and ENMs/cm(2). Results: Studied Ag ENMs are cytotoxic and cytostatic,
and induced strand breaks, DNA oxidation, inflammation and gene mutations. Results
expressed in mass unit [mu g/cm(2)] suggested that the toxicity of Ag ENMs is size
dependent with 50 nm being most toxic. However, re-calculation of Ag ENMs
concentrations from mass unit to surface area and number of ENMs per cm(2)
highlighted that 200 nm Ag ENMs, are the most toxic. Results from hprt gene
mutation assay showed that Ag ENMs 200 nm are the most mutagenic irrespective of
the concentration unit expressed. Conclusion: We found that the toxicity of Ag ENMs
is not always size dependent. Strong cytotoxic and genotoxic effects were observed
in cells exposed to Ag ENMs 50 nm, but Ag ENMs 200 nm had the most mutagenic
potential. Additionally, we showed that expression of concentrations of ENMs in
mass units is not representative. Number of ENMs or surface area of ENMs (per
cm(2)) seem more precise units with which to compare the toxicity of different
ENMs.
AN - rayyan-553780617
AU - Huk, A.
AU - Izak-Nau, E.
AU - Reidy, B.
AU - Boyles, M.
AU - Duschl, A.
AU - Lynch, I.
AU - Dusinska, M.
DO - 10.1186/s12989-014-0065-1
PY - 2014
SN - 1743-8977
ST - Is the toxic potential of nanosilver dependent on its size?
TI - Is the toxic potential of nanosilver dependent on its size?
VL - 11
Y2 - 12 y3 - 3
ID - 8644
ER -
TY - JOUR
AB - BACKGROUND: Nanosilver is one of the most commonly used engineered
nanomaterials (ENMs). In our study we focused on assessing the size-dependence of
the toxicity of nanosilver (Ag ENMs), utilising materials of three sizes (50, 80
and 200 nm) synthesized by the same method, with the same chemical composition,
charge and coating. METHODS: Uptake and localisation (by Transmission Electron
Microscopy), cell proliferation (Relative growth activity) and cytotoxic effects
(Plating efficiency), inflammatory response (induction of IL-8 and MCP-1 by Enzyme
linked immune sorbent assay), DNA damage (strand breaks and oxidised DNA lesions by
the Comet assay) were all assessed in human lung carcinoma epithelial cells (A549),
and the mutagenic potential of ENMs (Mammalian hprt gene mutation test) was
assessed in V79-4 cells as per the OECD protocol. Detailed physico-chemical
characterization of the ENMs was performed in water and in biological media as a
prerequisite to assessment of their impacts on cells. To study the relationship
between the surface area of the ENMs and the number of ENMs with the biological
response observed, Ag ENMs concentrations were recalculated from μg/cm2 to ENMs
cm2/cm2 and ENMs/cm2. RESULTS: Studied Ag ENMs are cytotoxic and cytostatic, and
induced strand breaks, DNA oxidation, inflammation and gene mutations. Results
expressed in mass unit [μg/cm2] suggested that the toxicity of Ag ENMs is size
dependent with 50 nm being most toxic. However, re-calculation of Ag ENMs
concentrations from mass unit to surface area and number of ENMs per cm2
highlighted that 200 nm Ag ENMs, are the most toxic. Results from hprt gene
mutation assay showed that Ag ENMs 200 nm are the most mutagenic irrespective of
the concentration unit expressed. CONCLUSION: We found that the toxicity of Ag ENMs
is not always size dependent. Strong cytotoxic and genotoxic effects were observed
in cells exposed to Ag ENMs 50 nm, but Ag ENMs 200 nm had the most mutagenic
potential. Additionally, we showed that expression of concentrations of ENMs in
mass units is not representative. Number of ENMs or surface area of ENMs (per cm2)
seem more precise units with which to compare the toxicity of different ENMs.
AN - rayyan-553782016
AU - Huk, A.
AU - Izak-Nau, E.
AU - Reidy, B.
AU - Boyles, M.
AU - Duschl, A.
AU - Lynch, I.
AU - Dušinska, M.
DO - 10.1186/s12989-014-0065-1
KW - Animals
Cell Line, Tumor
Cell Proliferation/drug effects
Cell Shape/drug effects
Chemokine CCL2/metabolism
Cricetinae
DNA Damage
Humans
Inflammation Mediators/metabolism
Interleukin-8/metabolism
Lung/*drug effects/immunology/metabolism/pathology
Mutation
Oxidative Stress/drug effects
Particle Size
Risk Assessment
Silver/chemistry/metabolism/*toxicity
Surface Properties
Time Factors
LA - eng
PY - 2014
SP - 65
ST - Is the toxic potential of nanosilver dependent on its size?
TI - Is the toxic potential of nanosilver dependent on its size?
VL - 11
Y2 - 12 y3 - 3
ID - 9932
ER -
TY - JOUR
AB - The engineering of vascular regeneration still involves barriers that need to
be conquered. In the current study, a novel nanocomposite comprising of fibronectin
(denoted as FN) and a small amount of silver nanoparticles (AgNP, ~15.1, ~30.2 or
~75.5 ppm) was developed and its biological function and biocompatibility in
Wharton’s jelly‐derived mesenchymal stem cells (MSCs) and rat models was
investigated. The surface morphology as well as chemical composition for pure FN
and the FN‐AgNP nanocomposites incorporating various amounts of AgNP were firstly
characterized by atomic force microscopy (AFM), UV‐Visible spectroscopy (UV‐Vis),
and Fourier‐transform infrared spectroscopy (FTIR). Among the nanocomposites, FN‐
AgNP with 30.2 ppm silver nanoparticles demonstrated the best biocompatibility as
assessed through intracellular ROS production, proliferation of MSCs, and monocytes
activation. The expression levels of pro‐inflammatory cytokines, TNF‐α, IL‐1β, and
IL‐6, were also examined. FN‐AgNP 30.2 ppm significantly inhibited pro‐inflammatory
cytokine expression compared to other materials, indicating superior performance of
anti‐immune response. Mechanistically, FN‐AgNP 30.2 ppm significantly induced
greater expression of vascular endothelial growth factor (VEGF) and stromal‐cell
derived factor‐1 alpha (SDF‐1α) and promoted the migration of MSCs through matrix
metalloproteinase (MMP) signaling pathway. Besides, in vitro and in vivo studies
indicated that FN‐AgNP 30.2 ppm stimulated greater protein expressions of CD31 and
von Willebrand Factor (vWF) as well as facilitated better endothelialization
capacity than other materials. Furthermore, the histological tissue examination
revealed the lowest capsule formation and collagen deposition in rat subcutaneous
implantation of FN‐ AgNP 30.2 ppm. In conclusion, FN‐AgNP nanocomposites may
facilitate the migration and proliferation of MSCs, induce endothelial cell
differentiation, and attenuate immune response. These finding also suggests that
FN‐AgNP may be a potential anti‐inflammatory surface modification strategy for
vascular biomaterials. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780619
AU - Hung, H. S.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Ku, T. R.
AU - Kung, M. L.
AU - Yu, A. Y. H.
AU - Shen, C. C.
AU - Yang, Y. C.
AU - Hsieh, H. H.
AU - Hsu, S. H.
DO - 10.3390/ijms22179262
IS - 17
KW - Endothelial differentiation
Fibronectin
Vascular tissue engineering
Animals
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cytoskeleton
Endothelial Cells
Fibronectins
Mesenchymal Stem Cells
Metal Nanoparticles
Particle Size
Rats
Silver
CD68 antigen
collagen
dimer
fibronectin
interleukin 16
interleukin 1beta
nanocomposite
silver nanoparticle
stromal cell derived factor 1alpha
vasculotropin
metal nanoparticle
silver
animal cell
animal experiment
animal model
Article
biocompatibility
cell activation
cell migration
cell proliferation
controlled study
cultural anthropology
endothelium cell
female
fibroblast
foreign body reaction
histology
immune response
in vitro study
in vivo study
macrophage
Masson staining
monocyte
nonhuman
particle size
polarization
protein expression
protein expression level
rat
signal transduction
surface property
animal
cell culture
cytology
cytoskeleton
drug effect
metabolism
ultrastructure
PY - 2021
ST - Anti‐inflammatory fibronectin‐agnp for regulation of biological performance
and endothelial differentiation ability of mesenchymal stem cells
TI - Anti‐inflammatory fibronectin‐agnp for regulation of biological performance
and endothelial differentiation ability of mesenchymal stem cells
85113466909&doi=10.3390%2fijms22179262&partnerID=40&md5=dd025605dd1ce0a82ffd7974b88
7ea1c
VL - 22
ID - 8645
ER -
TY - JOUR
AB - A nanocomposite composed of polyethylene glycol (PEG) incorporated with
various concentrations (~17.4, ~43.5, ~174 ppm) of gold nanoparticles (Au) was
created to investigate its biocompatibility and biological performance in vitro and
in vivo. First, surface topography and chemical composition was determined through
UV-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR),
atomic force microscopy (AFM), scanning electron microscopy (SEM), free radical
scavenging ability, and water contact angle measurement. Additionally, the
diameters of the PEG-Au nanocomposites were also evaluated through dynamic light
scattering (DLS) assay. According to the results, PEG containing 43.5 ppm of Au
demonstrated superior biocompatibility and biological properties for mesenchymal
stem cells (MSCs), as well as superior osteogenic differentiation, adipocyte
differentiation, and, particularly, neuronal differentiation. Indeed, PEG-Au 43.5
ppm induced better cell adhesion, proliferation and migration in MSCs. The higher
expression of the SDF-1 alpha/CXCR4 axis may be associated with MMPs activation and
may have also promoted the differentiation capacity of MSCs. Moreover, it also
prevented MSCs from apoptosis and inhibited macrophage and platelet activation, as
well as reactive oxygen species (ROS) generation. Furthermore, the anti-
inflammatory, biocompatibility, and endothelialization capacity of PEG-Au was
measured in a rat model. After implanting the nanocomposites into rats
subcutaneously for 4 weeks, PEG-Au 43.5 ppm was able to enhance the anti-immune
response through inhibiting CD86 expression (M1 polarization), while also reducing
leukocyte infiltration (CD45). Moreover, PEG-Au 43.5 ppm facilitated CD31
expression and anti-fibrosis ability. Above all, the PEG-Au nanocomposite was
evidenced to strengthen the differentiation of MSCs into various cells, including
fat, vessel, and bone tissue and, particularly, nerve cells. This research has
elucidated that PEG combined with the appropriate amount of Au nanoparticles could
become a potential biomaterial able to cooperate with MSCs for tissue regeneration
engineering.
AN - rayyan-553780620
AU - Hung, H. S.
AU - Kao, W. C.
AU - Shen, C. C.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Yang, M. Y.
AU - Yang, Y. C.
AU - Yeh, C. A.
AU - Li, J. J.
AU - Hsieh, H. H.
DO - 10.3390/cells10112854
IS - 11
KW - Polyethylene Glycols
PY - 2021
SN - 2073-4409
ST - Inflammatory Modulation of Polyethylene Glycol-AuNP for Regulation of the
Neural Differentiation Capacity of Mesenchymal Stem Cells
T2 - CELLS
TI - Inflammatory Modulation of Polyethylene Glycol-AuNP for Regulation of the
Neural Differentiation Capacity of Mesenchymal Stem Cells
VL - 10
Y2 - 11
ID - 8646
ER -
TY - JOUR
AB - Cardiovascular Diseases (CVDs) such as atherosclerosis, where inflammation
occurs in the blood vessel wall, are one of the major causes of death worldwide.
Mesenchymal Stem Cells (MSCs)-based treatment coupled with nanoparticles is
considered to be a potential and promising therapeutic strategy for vascular
regeneration. Thus, angiogenesis enhanced by nanoparticles is of critical concern.
In this study, Polyethylene Glycol (PEG) incorporated with 43.5 ppm of gold (Au)
nanoparticles was prepared for the evaluation of biological effects through in
vitro and in vivo assessments. The physicochemical properties of PEG and PEG-Au
nanocomposites were first characterized by UV-Vis spectrophotometry (UV-Vis),
Fourier-transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFMs).
Furthermore, the reactive oxygen species scavenger ability as well as the
hydrophilic property of the nanocomposites were also investigated. Afterwards, the
biocompatibility and biological functions of the PEG-Au nanocomposites were
evaluated through in vitro assays. The thin coating of PEG containing 43.5 ppm of
Au nanoparticles induced the least platelet and monocyte activation. Additionally,
the cell behavior of MSCs on PEG-Au 43.5 ppm coating demonstrated better cell
proliferation, low ROS generation, and enhancement of cell migration, as well as
protein expression of the endothelialization marker CD31, which is associated with
angiogenesis capacity. Furthermore, anti-inflammatory and endothelial
differentiation ability were both evaluated through in vivo assessments. The
evidence demonstrated that PEG-Au 43.5 ppm implantation inhibited capsule formation
and facilitated the expression of CD31 in rat models. TUNEL assay also indicated
that PEG-Au nanocomposites would not induce significant cell apoptosis. The above
results elucidate that the surface modification of PEG-Au nanomaterials may enable
them to serve as efficient tools for vascular regeneration grafts.
AN - rayyan-553780621
AU - Hung, H. S.
AU - Yang, Y. C.
AU - Kao, W. C.
AU - Yeh, C. A.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Hsieh, H. H.
AU - Lee, H. T.
DO - 10.3390/polym13234265
IS - 23
KW - Glycols
Polyethylenes
Polyethylene
PY - 2021
SN - 2073-4360
ST - Evaluation of the Biocompatibility and Endothelial Differentiation Capacity
of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites
T2 - POLYMERS
TI - Evaluation of the Biocompatibility and Endothelial Differentiation Capacity
of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites
VL - 13
Y2 - 12
ID - 8647
ER -
TY - JOUR
AB - The indications for use of programed cell death receptor (PD-1) inhibitors to
treat cancer continues to expand rapidly. Treatment with PD-1 inhibitors has been
associated with numerous immune-mediated mucocutaneous side effects. Here, we
report 2 cases of severe mucositis caused by the PD-1 inhibitor pembrolizumab and
review the defining features of similar cases. Recognition of mucocutaneous
toxicities of PD-1 inhibitors is increasingly important as their use continues to
expand. A stepwise approach to diagnosis and management is also reviewed. © 2020
American Association of Oral and Maxillofacial Surgeons
AN - rayyan-553780622
AU - Huntley, R. E.
AU - DeNiro, K.
AU - Yousef, J.
AU - Sheedy, M.
AU - Dillon, J. K.
DO - 10.1016/j.joms.2020.11.023
IS - 6
KW - Algorithms
Antibodies, Monoclonal
Antibodies, Monoclonal, Humanized
Humans
Mucositis
Programmed Cell Death 1 Receptor
aciclovir
chlorhexidine
dexamethasone
lidocaine
methenamine
mouthwash
nystatin
pembrolizumab
prednisone
monoclonal antibody
programmed death 1 receptor
adult
advanced cancer
aged
antifungal therapy
Article
basement membrane
behavior disorder
bladder cancer
body weight loss
buccal mucosa
cachexia
cancer immunotherapy
case report
cheilitis
clinical article
comparative study
drug substitution
drug withdrawal
eating
erosion
esophagogastroduodenoscopy
esophagus biopsy
eye examination
fingernail onychomycosis
gastroenterology
graft versus host reaction
granulation tissue
histology
human
hyperplasia
hypertransaminasemia
immunofluorescence
inflammation
infusion
lip
male
medical history
metastasis
middle aged
mouth mucosa
mouth pain
mouth ulcer
mucosa inflammation
non small cell lung cancer
odynophagia
oral biopsy
oral mucositis
oropharynx
outpatient department
parakeratosis
periodic acid Schiff stain
physical examination
punch biopsy
seizure
silver staining
skin biopsy
skin injury
skin necrosis
tongue ulcer
virus culture
algorithm
PY - 2021
SP - 1262-1269
ST - Severe Mucositis Secondary to Pembrolizumab: Reports of Two Cases, Review of
the Literature, and an Algorithm for Management
T2 - Journal of Oral and Maxillofacial Surgery
TI - Severe Mucositis Secondary to Pembrolizumab: Reports of Two Cases, Review of
the Literature, and an Algorithm for Management
85099665773&doi=10.1016%2fj.joms.2020.11.023&partnerID=40&md5=96f0b720dd84ff90da0b7
1bf97043fea
VL - 79
ID - 8648
ER -
TY - JOUR
AB - Owing to their tremendous potential, the inference of nano-scaled materials
has revolutionized many fields including the medicine and health, particularly for
development of various types of targeted drug delivery devices for early prognosis
and successful treatment of various diseases, including the brain disorders. Owing
to their unique characteristic features, a variety of nanomaterials (particularly,
ultra-fine particles (UFPs) have shown tremendous success in achieving the
prognostic and therapeutic goals for early prognosis and treatment of various brain
maladies such as Alzheimer's disease, Parkinson's disease, brain lymphomas, and
other ailments. However, serious attention is needful due to innumerable after-
effects of the nanomaterials. Despite their immense contribution in optimizing the
prognostic and therapeutic modalities, biological interaction of nanomaterials with
various body tissues may produce severe nanotoxicity of different organs including
the heart, liver, kidney, lungs, immune system, gastro-intestinal system, skin as
well as nervous system. However, in this review, we have primarily focused on
nanomaterials-induced neurotoxicity of the brain. Following their translocation
into different regions of the brain, nanomaterials may induce neurotoxicity through
multiple mechanisms including the oxidative stress, DNA damage, lysosomal
dysfunction, inflammatory cascade, apoptosis, genotoxicity, and ultimately necrosis
of neuronal cells. Our findings indicated that rigorous toxicological evaluations
must be carried out prior to clinical translation of nanomaterials-based
formulations to avoid serious neurotoxic complications, which may further lead to
develop various neuro-degenerative disorders.
AN - rayyan-553780623
AU - Hussain, Z.
AU - Thu, H. E.
AU - Elsayed, I.
AU - Abourehab, M. A. S.
AU - Khan, S.
AU - Sohail, M.
AU - Sarfraz, R. M.
AU - Farooq, M. A.
DO - 10.1016/j.jconrel.2020.10.053
KW - Brain
Causality
PY - 2020
SN - 0168-3659 1873-4995
SP - 873-894
ST - Nano-scaled materials may induce severe neurotoxicity upon chronic exposure
to brain tissues: A critical appraisal and recent updates on predisposing factors,
underlying mechanism, and future prospects
T2 - JOURNAL OF CONTROLLED RELEASE
TI - Nano-scaled materials may induce severe neurotoxicity upon chronic exposure
to brain tissues: A critical appraisal and recent updates on predisposing factors,
underlying mechanism, and future prospects
VL - 328
Y2 - 12 y3 - 10
ID - 8649
ER -
TY - JOUR
AB - Owing to their tunable physicochemical features and wide range of biomedical
applications, silver nanoparticles (SNPs) have been extensively investigated.
Despite exhibiting strong biomedical potential, therapeutic significance of SNPs is
limited, particularly when employed alone for treatment of chronic severe
conditions (infected diabetic wounds, cancer, etc.). These restrictions convinced
researchers to develop newer hybrid SNPs-based nanomaterials to fully replicate
characteristics of ideal nanomaterials. Therefore, the present review was aimed to
reconceptualise emerging adaptations such as biofabrication, functionalization, and
hybridization of SNPs with biological macromolecules in the form of nanocomposites,
sponges, hydrogel, scaffolds, electrospun nanofibers, and nanomembrane for
improvement of their biomedical implications. Functionalization of SNPs with
biological macromolecules showed promising improvement of their various biomedical
properties such as wound healing, tissue regeneration, antibacterial, antifungal,
anticancer, anti-inflammatory, antioxidant, and anti-diabetic. Similarly,
hybridization of SNPs with one or more biopolymers (chitosan, hyaluronic acid (HA),
cellulose, alginate, starch, etc.) has further improved their physicochemical
characteristics (morphology, tensile strength, and release kinetics),
biocompatibility, pharmacokinetic profile, and synergistic biomedical efficacy.
Recent developments and progress made in the designing of SNPs-based hybrid
nanomaterials, their superior biomedical efficacies, current challenges to their
clinical transition and future prospects, have also been reconceptualised. © 2019
Elsevier B.V.
AN - rayyan-553780624
AU - Hussain, Z.
AU - Thu, H. E.
AU - Sohail, M.
AU - Khan, S.
DO - 10.1016/j.jddst.2019.101169
KW - Biological macromolecules
Functionalization
Hybridization
Upgraded biomedical efficacy
biopolymer
silver nanoparticle
drug efficacy
human
hybridization
hydrogel
in vitro study
macromolecule
nonhuman
Review
tissue regeneration
wound healing
PY - 2019
ST - Hybridization and functionalization with biological macromolecules
synergistically improve biomedical efficacy of silver nanoparticles:
Reconceptualization of in-vitro, in-vivo and clinical studies
T2 - Journal of Drug Delivery Science and Technology
TI - Hybridization and functionalization with biological macromolecules
synergistically improve biomedical efficacy of silver nanoparticles:
Reconceptualization of in-vitro, in-vivo and clinical studies
85073098739&doi=10.1016%2fj.jddst.2019.101169&partnerID=40&md5=e3623359b9610d934cf5
3335f8a078f2
VL - 54
ID - 8650
ER -
TY - CHAP
AB - Nanoparticles (NPs) can be developed to improve drug penetration and reorient
chemotherapy, or selectively target the cancer cells or cell compartment. Both
passive and active targeting strategies are used to redirect the anticancer drugs.
Noble metals such as the silver NPs (AgNPs) are characterized by electrical,
optical, and thermal properties, and can be integrated into products for optical,
biological and chemical sensor applications such as pastes, conductive inks, and
fillers for high stabilization, electrical conductivity, and low sintering
temperatures. The biosynthesis of AgNPs, making use of bacteria, fungi,
actinomycetes, yeast, algae, and plants, is eco-friendly, green, nontoxic and
inexpensive. The AgNPs sytnhesized are of various shapes and sizes. The AgNPs
have diverse bioactivities including antibacterial, antifungal, antiviral, anti-
inflammatory, anti-angiogenic, and anticancer activities, with great potential for
use in cancer diagnosis and therapy. The mechanisms of AgNP-induced cytotoxicity
include endoplasmic reticulum stress, lactate dehydrogenase leakage, and enhanced
reactive oxygen species level. Co-application of AgNPs and natural products could
play an essential role in nanoscience and nanotechnology, especially in
nanomedicine for cancer diagnosis and therapeutics. © 2020, Springer Nature
Switzerland AG.
AN - rayyan-553780625
AU - Hussein, H. A.
AU - Abdullah, M. A.
DO - 10.1007/978-3-030-41464-1_14
KW - Anti-cancer
Nanobiotechnology
Nanocarrier
Nanomedicine
Silver Nanoparticles
PY - 2020
SP - 313-332
ST - Biosynthesis, Mechanisms, and Biomedical Applications of Silver Nanoparticles
T2 - Nanotechnology in the Life Sciences
TI - Biosynthesis, Mechanisms, and Biomedical Applications of Silver Nanoparticles
85102114308&doi=10.1007%2f978-3-030-41464-
1_14&partnerID=40&md5=01acf1b4e976fcd7fffbe6c29a279709
ID - 8651
ER -
TY - JOUR
AB - Here we developed a novel green synthesis method for gold nanoparticles (CGA-
AuNPs) using chlorogenic acid (CGA) as reductants without the use of other
chemicals and validated the anti-inflammatory efficacy of CGA-AuNPs in vitro and in
vivo. The resulting CGA-AuNPs appeared predominantly spherical in shape with an
average diameter of 22.25 +/- 4.78 nm. The crystalline nature of the CGA-AuNPs was
confirmed by high-resolution X-ray diffraction and by selected-area electron
diffraction analyses. High-resolution liquid chromatography/electrospray ionization
mass spectrometry revealed that the caffeic acid moiety of CGA forms quinone
structure through a two-electron oxidation causing the reduction of Au3+ to Au-0.
When compared to CGA, CGA-AuNPs exhibited enhanced anti-inflammatory effects on NF-
kappa B-mediated inflammatory network, as well as cell adhesion. Collectively,
green synthesis of CGA-AuNPs using bioactive reductants and mechanistic studies
based on mass spectrometry may open up new directions in nanomedicine and CGA-AuNPs
can be an anti-inflammatory nanomedicine for future applications. From the Clinical
Editor: Gold nanoparticles (Au NPs) have been shown to be very useful in many
applications due to their easy functionalization capability. In this article, the
authors demonstrated a novel method for the synthesis of gold nanoparticles using
chlorogenic acid (CGA) as reductants. In-vitro experiments also confirmed
biological activity of the resultant gold nanoparticles. Further in-vivo studies
are awaited. (C) 2015 Elsevier Inc. All rights reserved.
AN - rayyan-553780626
AU - Hwang, S. J.
AU - Jun, S. H.
AU - Park, Y.
AU - Cha, S. H.
AU - Yoon, M.
AU - Cho, S.
AU - Lee, H. J.
DO - 10.1016/j.nano.2015.05.002
IS - 7
KW - Inflammation
PY - 2015
SN - 1549-9634 1549-9642
SP - 1677-1688
ST - Green synthesis of gold nanoparticles using chlorogenic acid and their
enhanced performance for inflammation
T2 - NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
TI - Green synthesis of gold nanoparticles using chlorogenic acid and their
enhanced performance for inflammation
VL - 11
Y2 - 10
ID - 8652
ER -
TY - JOUR
AB - Background: Burns are considered a critical care problem in emergency
medicine, resulting in physical, psychological, and chronic disabilities. Silver
sulfadiazine is the gold standard in topical burn treatment but was associated with
toxicity to keratinocytes and fibroblasts, which may delay wound healing. In
discovering potential alternative treatments for burn wound healing, this study was
performed to determine the effect of Labisia Pumila (Blume) Fern.-Vill. Var. Alata
(LPVa) extract on thermal-burn wounds in rats. Methods: A total of 50 Sprague-
Dawley male rats were categorized into five groups. There were three control
groups; normal control (left untreated), negative control (given ointment base) and
positive control (given silver sulfadiazine). Meanwhile, the two intervention
groups were given with 2% LPVa leaf and root extracts, respectively. Burn wounds
were inflicted on the loin region of the rat by applying a heated steel rod at 80°C
for 10 s. On days 3, 7, 14, and 21, wounds were measured macroscopically using a
digital calliper and one animals of each group were sacrificed, and the wounded
skin were excised for histomorphological assessments. The wounds were excised for
hydroxyproline content on Day 14 of treatment. Result: For wound contraction
percentage, both the leaf and root extracts of LPVa showed a significant reduction
in burn wound size on Day 7 onwards, when compared to other groups. For
hydroxyproline content, only the leaf extract of LPVa produced significantly higher
content compared to both negative and normal control groups. In terms of
histological examination, the leaf extract group demonstrated a superior healing
effect than the root extract group. Conclusion: Both leaf and root extracts of LPVa
could promote wound healing in the thermal-burn wound rat model, with leaf extract
being superior to root extract. Copyright © 2022 Ibrahim, Mohamed, Mohamed, Mohd
Ramli and Shuid.
AN - rayyan-553780627
AU - Ibrahim, N. I.
AU - Mohamed, I. N.
AU - Mohamed, N.
AU - Mohd Ramli, E. S.
AU - Shuid, A. N.
DO - 10.3389/fphar.2022.968664
KW - antioxidant
burn wound healing
histomorphology
hydroxyproline
labisia pumila
labisia pumila extract
plant extract
sulfadiazine silver
unclassified drug
animal experiment
animal model
animal tissue
area under the curve
Article
burn model
cell infiltration
controlled study
data analysis
gold standard
histology
infant
inflammatory cell
male
model
morphology
nonhuman
photography
rat
thickness
toxicity
wound contraction
wound healing
Burns
Hydroxyproline
PY - 2022
ST - The effects of aqueous extract of Labisia Pumila (Blume) Fern.-Vill. Var.
Alata on wound contraction, hydroxyproline content and histological assessments in
superficial partial thickness of second-degree burn model
T2 - Frontiers in Pharmacology
TI - The effects of aqueous extract of Labisia Pumila (Blume) Fern.-Vill. Var.
Alata on wound contraction, hydroxyproline content and histological assessments in
superficial partial thickness of second-degree burn model
85140587506&doi=10.3389%2ffphar.2022.968664&partnerID=40&md5=d649d3958ec403bd1cf7a8
853e73823b
VL - 13
ID - 8653
ER -
TY - JOUR
AB - Bryonia alba L. is the only Bryonia species found in Romanian flora, being
known as a remedy for inflammatory pathologies or for its hepatoprotective and
adaptogen activities. The present investigation studied the flavonoid composition
and antioxidant activities of the aerial parts of this species. Flavonoid profile
was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant
capacity was assessed by various methods, testing di erent antioxidant mechanisms:
DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant
capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent
antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC
(silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human
cancerous and healthy cell lines. Anti-plasmodial tests were performed on two
strains of Plasmodium falciparum. Whole organism toxicity was assessed on zebrafish
larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin,
isoorientin, and isovitexin as the major flavonoids in the composition of tested
samples. Significant results were obtained for all antioxidant capacity assays. The
cytotoxicity tests proved the absence of cellular and parasitic toxicity and these
results were confirmed by the lack of toxicity on the zebrafish larvae model. This
study proves a promising potential of the aerial parts of Bryonia alba L. as
antioxidant agents. © 2019 by the authors.
AN - rayyan-553780628
AU - Ielciu, I.
AU - Frédérich, M.
AU - Hanganu, D.
AU - Angenot, L.
AU - Olah, N. K.
AU - Ledoux, A.
AU - Crisan, G.
AU - Paltinean, R.
DO - 10.3390/antiox8040108
IS - 4
KW - Antioxidant
Flavonoids
HPLC-DAD
Toxicity
Antioxidants
PY - 2019
ST - Flavonoid analysis and antioxidant activities of the bryonia alba L. Aerial
parts
T2 - Antioxidants
TI - Flavonoid analysis and antioxidant activities of the bryonia alba L. Aerial
parts
85070662957&doi=10.3390%2fantiox8040108&partnerID=40&md5=caf2ea1184b7f57887e9f6742c
3e51ec
VL - 8
ID - 8654
ER -
TY - JOUR
AB - Bryonia alba L. is the only Bryonia species found in Romanian flora, being
known as a remedy for inflammatory pathologies or for its hepatoprotective and
adaptogen activities. The present investigation studied the flavonoid composition
and antioxidant activities of the aerial parts of this species. Flavonoid profile
was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant
capacity was assessed by various methods, testing different antioxidant mechanisms:
DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant
capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent
antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC
(silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human
cancerous and healthy cell lines. Anti-plasmodial tests were performed on two
strains of Plasmodium falciparum. Whole organism toxicity was assessed on zebrafish
larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin,
isoorientin, and isovitexin as the major flavonoids in the composition of tested
samples. Significant results were obtained for all antioxidant capacity assays. The
cytotoxicity tests proved the absence of cellular and parasitic toxicity and these
results were confirmed by the lack of toxicity on the zebrafish larvae model. This
study proves a promising potential of the aerial parts of Bryonia alba L. as
antioxidant agents.
AN - rayyan-553780629
AU - Ielciu, I.
AU - Frederich, M.
AU - Hanganu, D.
AU - Angenot, L.
AU - Olah, N. K.
AU - Ledoux, A.
AU - Crisan, G.
AU - Paltinean, R.
DO - 10.3390/antiox8040108
IS - 4
KW - Antioxidants
Flavonoids
PY - 2019
SN - 2076-3921
ST - Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial
Parts
T2 - ANTIOXIDANTS
TI - Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial
Parts
VL - 8
Y2 - 4
ID - 8655
ER -
TY - JOUR
AB - As a chronic inflammatory disease, atherosclerosis is responsible for
thousands of deaths worldwide each year, and it imposes massive economic costs on
individuals and on society. Because of its high importance, the discovery of
sensitive and accurate strategies for imaging, targeted drug delivery, and
therapeutic monitoring of this condition is essential. In recent years, continuous
research has achieved remarkable successes in the use of nanotechnology in the
molecular imaging and treatment of atherosclerosis. Among various nanoparticles -
such as metallic, polymeric, and lipid - metallic nanoparticles are being
considered due to their unique properties for use in treatment and imaging. It
should be taken into consideration that some of the metal nanoparticles themselves
can cause adverse biological effects, and these effects should be considered
important risk factors in the pathological pathways leading up to atherosclerosis.
This review provides a description of the applications and potential toxicity of
metal nanoparticles in atherosclerosis. © 2015 Fatemeh Imanparast, Mahmood Doosti
and Mohammad Ali Faramarzi.
AN - rayyan-553780635
AU - Imanparast, F.
AU - Doosti, M.
AU - Faramarzi, M. A.
DO - 10.5101/nbe.v7i3.p111-127
IS - 3
KW - Atherosclerosis
Drug delivery
Imaging
Metals
Nanoparticles
Toxicity
colony stimulating factor
gamma interferon
gold nanoparticle
metal nanoparticle
nickel nanoparticle
oxidized low density lipoprotein
PADGEM protein
platelet derived growth factor
silver nanoparticle
superparamagnetic iron oxide nanoparticle
antiangiogenic activity
atherogenesis
atherosclerosis
atherosclerotic plaque
blood clotting
blood vessel rupture
cell migration
cell proliferation
disease association
endothelial dysfunction
endothelium cell
endothelium injury
foam cell
inflammation
macrophage
molecular imaging
molecular pathology
monocyte
nanomedicine
nanotoxicology
neutrophil
Review
thrombocyte adhesion
tight junction
vascular smooth muscle cell
Metal Nanoparticles
PY - 2015
SP - 111-127
ST - Metal nanoparticles in atherosclerosis: Applications and potential toxicity
T2 - Nano Biomedicine and Engineering
TI - Metal nanoparticles in atherosclerosis: Applications and potential toxicity
84945194856&doi=10.5101%2fnbe.v7i3.p111-
127&partnerID=40&md5=bdb949ff78f5994cd754aeac26fe907e
VL - 7
ID - 8661
ER -
TY - JOUR
AB - Partial-thickness burn injury has the potential for reepithelialization and
heals within 3 weeks. If the wound is infected by bacteria before reepithelization,
however, the depth of disruption increases and the lesion easily progresses to the
full-thickness dermal layers. In the treatment of partial-thickness burn injury, it
is important to prevent the wound area from bacterial infection with an
antimicrobial dressing. Here, we have tested the antimicrobial properties of
polymeric ultra-thin films composed of poly(lactic acid) (termed "PLA nanosheets"),
which have high flexibility, adhesive strength and transparency, and silver
sulfadiazine (AgSD), which exhibits antimicrobial efficacy. The AgSD-loaded
nanosheet released Ag+ for more than 3 days, and exerted antimicrobial efficacy
against methicillin-resistant Staphylococcus aureus (MRSA) in an in vitro Kirby-
Bauer test. By contrast, a cell viability assay indicated that the dose of AgSD
used in the PLA nanosheets did not show significant cytotoxicity toward
fibroblasts. In vivo evaluation using a mouse model of infection in a partial-
thickness burn wound demonstrated that the nanosheet significantly reduced the
number of MRSA bacteria on the lesion (more than 105-fold) and suppressed the
inflammatory reaction, thereby preventing a protracted wound healing process. ©
2015 Acta Materialia Inc.
AN - rayyan-553780639
AU - Ito, K.
AU - Saito, A.
AU - Fujie, T.
AU - Nishiwaki, K.
AU - Miyazaki, H.
AU - Kinoshita, M.
AU - Saitoh, D.
AU - Ohtsubo, S.
AU - Takeoka, S.
DO - 10.1016/j.actbio.2015.05.035
KW - Antimicrobial
Poly(lactic acid)
Polymer nanosheet
Silver sulfadiazine
Wound dressing
Animals
Burns
Methicillin-Resistant Staphylococcus aureus
Mice
Nanoparticles
Silver Sulfadiazine
Staphylococcal Infections
Wound Healing
Bacteria (microorganisms)
Cell culture
Lactic acid
Mammals
Nanosheets
Silver
Ultrathin films
polylactic acid
sulfadiazine silver
antiinfective agent
nanoparticle
Anti-microbial effects
Burn injury
Effect of silvers
Methicillin-resistant staphylococcus aureus
Mice models
Poly lactic acid
Polymer nanosheets
Silver sulfadiazines
Wound dressings
animal experiment
animal model
Article
burn infection
cell viability assay
cytotoxicity
drug efficacy
drug release
drug screening
fibroblast
film
in vitro study
in vivo study
inflammation
nonhuman
polymerization
priority journal
wound healing
animal
chemistry
drug effects
microbiology
mouse
Bacteria
PY - 2015
SP - 87-95
ST - Sustainable antimicrobial effect of silver sulfadiazine-loaded nanosheets on
infection in a mouse model of partial-thickness burn injury
T2 - Acta Biomaterialia
TI - Sustainable antimicrobial effect of silver sulfadiazine-loaded nanosheets on
infection in a mouse model of partial-thickness burn injury
84940450427&doi=10.1016%2fj.actbio.2015.05.035&partnerID=40&md5=ffb8d6b489881536d35
3b69a168ef64e
VL - 24
ID - 8665
ER -
TY - JOUR
AB - Background Radiotherapy is a common cancer treatment, but often results in
unintended injury to overlying skin and contributes to poor wound healing. The
mechanisms underlying these changes are complex, and existing treatment is limited.
We aimed to systematically review the literature on the pathogenesis, management,
and experimental treatment of delayed wound healing following radiation therapy.
Study Design/Methods A literature search was performed following PRISMA-P
guidelines. PubMed and the Cochrane Library were queried for full-text English
articles published up to 2016 by using key search terms including “radiotherapy”
and “wound healing.” Additional resources were retrieved from reference lists. The
selected articles discussed mechanisms of pathogenesis, current management, and
experimental treatment of radiation-induced skin injury. These are reported as a
qualitative synthesis of the literature from the authors' perspective. Results The
literature search yielded 442 articles, of which 93 were included in this review.
Ionizing radiation causes DNA damage by direct strand breaks and oxygen-derived
free radicals. This insult results in cellular alterations that underlie mechanisms
of delayed wound healing. Prominent theories underlying pathogenesis include
cellular depletion, stromal cell dysfunction, aberrant collagen deposition, and
microvascular damage. Pro-inflammatory cytokines and free radical cascades
contribute to chronic radiation damage, with transforming growth factor beta-1
(TGF-β1) implicated as a key player in the process of fibrosis. At present,
radiation injury is managed medically with conventional wound care with minimal
success and limited evidence-based data. Surgical management with local or free
flap transfer is often compromised by poor surrounding tissue and recipient
vasculature. Experimental treatment models are emerging that target mechanisms of
pathogenesis. These modalities include stem cell repletion, antioxidant therapy,
TGF-β1 modulation, and implantable biomaterials. Conclusion Pathogenesis of delayed
wound healing and fibrosis following radiotherapy is a complex, interdependent
process involving cellular depletion, extracellular matrix changes, microvascular
damage, and altered pro-inflammatory mediators. Current treatment is limited, and
more Level I studies are needed to develop best-practice recommendations.
Investigatory treatment options targeting specific mechanisms of injury may offer
potential solutions to this significant clinical and surgical problem. © 2017 The
Author(s)
AN - rayyan-553780642
AU - Jacobson, L. K.
AU - Johnson, M. B.
AU - Dedhia, R. D.
AU - Niknam-Bienia, S.
AU - Wong, A. K.
DO - 10.1016/j.jpra.2017.04.001
KW - Ionizing radiation
Radiation fibrosis
Radiation therapy
Skin
Systematic review
Wound healing
Aloe vera extract
alpha tocopherol
amifostine
ascorbic acid
becaplermin
biomaterial
Calendula extract
corticosteroid derivative
cytokine
DNA
free radical
gamma interferon
halofuginone
histone deacetylase inhibitor
hyaluronic acid
interleukin 1
interleukin 6
nitric oxide
oxygen
pentoxifylline
prostaglandin E1
proteinase
sucralfate
sulfadiazine silver
triethanolamine
vasculotropin
zinc
apoptosis
Article
debridement
DNA damage
DNA strand breakage
experimental therapy
free tissue graft
human
hydrocolloid dressing
hydrogel dressing
hyperbaric oxygen therapy
ionizing radiation
microangiopathy
nonhuman
pathogenesis
practice guideline
qualitative analysis
radiation dermatitis
radiation injury
radiotherapy
skin fibrosis
skin injury
skin protection
stroma cell
systematic review (topic)
tissue flap
treatment response
wound care
Wound Healing
PY - 2017
SP - 92-105
ST - Impaired wound healing after radiation therapy: A systematic review of
pathogenesis and treatment
T2 - JPRAS Open
TI - Impaired wound healing after radiation therapy: A systematic review of
pathogenesis and treatment
85024366523&doi=10.1016%2fj.jpra.2017.04.001&partnerID=40&md5=8d7d8e090ca302513289e
79d39d85ba8
VL - 13
ID - 8668
ER -
TY - JOUR
AB - Tuberculosis (TB) is a highly contagious infection with extensive mortality
and morbidity. The rise of TB-superbugs (drug-resistant strains) with the increase
of their resistance to conventional antibiotics has prompted a further search for
new anti-mycobacterial agents. It is difficult to breach the barriers around TB
bacteria, including mycolic cell wall, granuloma, biofilm and mucus, by
conventional antibiotics in a short span of time. Hence, there is an essential need
for molecules with an uncon-ventional mode of action and structure that can
efficiently break the barriers around mycobacterium. Antimicrobial peptides (AMP)
are essential components of innate immunity having cationic and am-phipathic
characteristics. Lines of evidence show that AMPs have good myco-bactericidal and
anti-biofilm activity against normal as well as antibiotic-resistant TB bacteria.
These peptides have shown direct killing of bacteria by membrane lysis and indirect
killing by activation of innate immune response in host cells by interacting with
the component of the bacterial membrane and intracellular targets through diverse
mechanisms. Despite a good anti-mycobacterial activity, some undesirable
characteristics are also associated with AMP, including hemolysis, cytotoxicity,
susceptibility to proteolysis and poor pharmacokinetic profile, and hence only a
few clinical studies have been conducted with these biomolecules. The design of new
combinatorial therapies, including AMPs and particulate drug delivery systems,
could be new potential alternatives to conventional antibiotics to fight MDR-and
XDR-TB. This review outlined the array of AMP roles in TB therapy, possible
mechanisms of actions, activi-ties, and current advances in pragmatic strategies to
improve challenges accompanying the delivery of AMP for tuberculosis therapeutics.
© 2022 Bentham Science Publishers.
AN - rayyan-553780643
AU - Jadhav, K.
AU - Singh, R.
AU - Ray, E.
AU - Singh, A. K.
AU - Verma, R. K.
DO - 10.2174/1389203723666220526161109
IS - 10
KW - Antimicrobial peptides
drug delivery
microparticles
nanoparticles
pro-inflammatory
strategies
tuberculosis
Adenosine Monophosphate
Antimicrobial Cationic Peptides
Antimicrobial Peptides
Bacteria
Humans
Tuberculosis
agents affecting metabolism
antibiotic agent
antimicrobial peptide inducer
aurien1 2 peptide
bacteriocin
beta defensin 1
bictcu5 peptide
bovine neutrophil beta defensin 5 peptide
cationic peptoid
d lak 120
d lak 120 a peptide
d lak 120 hp13 peptide
drug carrier
gold nanoparticle
hh2 peptide
hyaluronic acid nanogel
idr 1018 peptide
indolicidin
inorganic nanoparticle
ip 1 peptide
k4 peptide
lacticin 3147
lassomycin
liposome
llkkk18 peptide
m llkk 2m peptide
magainin 1 analog peptide
mesoporous silica nanoparticle
metal nanoparticle
microsphere
nanogel
nisin
nk 2 peptide
nzx peptide
pep 2 peptide
pin2 peptide
polyglactin microparticle
polyglactin nanoparticle
polymeric microparticle
polypeptide antibiotic agent
porous nano particles aggregates
pr 39
protegrin 1
ropocamptide
silver nanoparticle
trichoderin a
ub2 peptide
unclassified drug
vpamp2 0 peptide
wkwlkkwik peptide
adenosine phosphate
antiinfective agent
antimicrobial cationic peptide
antitubercular activity
drug formulation
dry powder
human
hybrid nano in micro formulation
immunomodulation
Mycobacterium bovis
Mycobacterium marinum
Mycobacterium smegmatis
nonhuman
Short Survey
synergistic effect
bacterium
chemistry
C-Peptide
Peptide T
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Peptide PHI
Peptides
PY - 2022
SP - 643-656
ST - Taming the Devil: Antimicrobial Peptides for Safer TB Therapeutics
T2 - Current Protein and Peptide Science
TI - Taming the Devil: Antimicrobial Peptides for Safer TB Therapeutics
85140481751&doi=10.2174%2f1389203723666220526161109&partnerID=40&md5=72abc235b05735
23d05a09cf20f44788
VL - 23
ID - 8669
ER -
TY - JOUR
AB - According to the World Health Organization report, the increasing antibiotic
resistance of microorganisms is one of the biggest global health problems. The
percentage of bacterial strains showing multidrug resistance (MDR) to commonly used
antibiotics is growing rapidly. Therefore, the search for alternative solutions to
antibiotic therapy has become critical to combat this phenomenon. It is especially
important as frequent and recurring infections can cause cancer. One example of
this phenomenon is urinary tract infections that can contribute to the development
of human urinary bladder carcinoma. This tumor is one of the most common malignant
neoplasms in humans. It occurs almost three times more often in men than in women,
and in terms of the number of cases, it is the fifth malignant neoplasm after
prostate, lung, colon, and stomach cancer. The risk of developing the disease
increases with age. Despite the improvement of its treatment methods, the current
outcome in the advanced stages of this tumor is not satisfactory. Hence, there is
an urgent need to introduce innovative solutions that will prove effective even in
the advanced stage of the disease. In our study, a nanosystem based on ionic silver
(Ag(+)) bound to a carrier-Titan yellow (TY) was analyzed. The possibility of
binding the thus formed TY-Ag system to Congo red (CR) and albumin (BSA) was
determined. TY-Ag binding to CR provides for better nanosystem solubility and
enables its targeted intracellular transport and binding to immune complexes. The
binding of TY-Ag or CR-TY-Ag to albumin also protects the system against the
uncontrolled release of silver ions. It will also allow the delivery of silver in a
targeted manner directly to the desired site in the case of intravenous
administration of such a system. In this study, the MIC (Minimum Inhibitory
Concentration) and MBC (Minimum Bactericidal Concentration) values of the TY-Ag or
BSA-TY-Ag systems were determined in two reference strains (Escherichia coli and
Staphylococcus aureus). The paper presents nanosystems with a size of about 40-50
nm, with an intense antibacterial effect obtained at concentrations of 0.019 mM. We
have also discovered that TY-Ag free or complexed with BSA (with a minimal Ag(+)
dose of 15-20 μM) inhibited cancer cells proliferation. TY-Ag complex diminished
migration and effectively inhibited the T24 cell viability and induced apoptosis.
On the basis of the obtained results, it has been shown that the presented systems
may have anti-inflammatory and antitumor properties at the same time. TY-Ag or BSA-
TY-Ag are new potential drugs and may become in future important therapeutic
compounds in human urinary bladder carcinoma treatment and/or potent antimicrobial
factors as an alternative to antibiotics.
AN - rayyan-553782259
AU - Jagusiak, A.
AU - Gosiewski, T.
AU - Romaniszyn, D.
AU - Lasota, M.
AU - Wiśniewska, A.
AU - Chłopaś, K.
AU - Ostrowska, B.
AU - Kościk, I.
AU - Bulanda, M.
DO - 10.3390/ijms23010026
IS - 1
J2 - Int J Mol Sci
KW - Albumins/*pharmacology
Anti-Bacterial Agents/*pharmacology
Antineoplastic Agents/pharmacology
Bacterial Infections/*drug therapy
Cell Line, Tumor
Congo Red/*pharmacology
Escherichia coli/drug effects
Humans
Ions/*pharmacology
Microbial Sensitivity Tests/methods
Silver/*pharmacology
Staphylococcus aureus/drug effects
Triazenes/*pharmacology
Urinary Bladder Neoplasms/drug therapy/*microbiology
Urinary Bladder Neoplasms
LA - eng
N1 - Chair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University
Medical College, 31-034 Kraków, Poland.; Chair of Microbiology, Department of
Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University
Medical College, 31-121 Krakow, Poland.; Chair of Microbiology, Department of
Infection Control and Mycology, Faculty of Medicine, Jagiellonian University
Medical College, 31-121 Krakow, Poland.; Chair of Medical Biochemistry, Faculty of
Medicine, Jagiellonian University Medical College, 31-034 Kraków, Poland.; Chair of
Pharmacology, Faculty of Medicine, Jagiellonian University Medical College, 31-531
Krakow, Poland.; Pulmonology and Allergology Clinical Department, University
Hospital in Krakow, 30-688 Krakow, Poland.; Chair of Medical Biochemistry, Faculty
of Medicine, Jagiellonian University Medical College, 31-034 Kraków, Poland.; Chair
of Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical
College, 31-034 Kraków, Poland.; Chair of Microbiology, Department of Infection
Control and Mycology, Faculty of Medicine, Jagiellonian University Medical College,
31-121 Krakow, Poland.
PY - 2021
ST - Antibacterial Therapy by Ag(+) Ions Complexed with Titan Yellow/Congo Red and
Albumin during Anticancer Therapy of Urinary Bladder Cancer
T2 - International journal of molecular sciences
TI - Antibacterial Therapy by Ag(+) Ions Complexed with Titan Yellow/Congo Red and
Albumin during Anticancer Therapy of Urinary Bladder Cancer
VL - 23
Y2 - 12 y3 - 21
ID - 10169
ER -
TY - JOUR
AB - Aim: To evaluate the anti inflammatory activity of silver nanoparticles
synthesised using Cumin oil. Introduction: Silver nanoparticles are the most
generally utilised nanoparticles both in key therapeutic sciences and clinical
practice. Silver particles are connected to modified cell demise, and expanded
cytotoxicity in specific conditions. Materials and method: Preparation of
denaturation particles using Cumin oil and evaluating the anti inflammatory
activity by using albumin denaturation assay technique. Results: The characteristic
surface plasmon absorption band were observed at 440nm. It is observed that with
the increase in concentration, the anti-inflammatory activity of silver
nanoparticles increases. Conclusion: Silver nanoparticles synthesized using cumin
oil exhibited potent anti inflammatory activity and hence can be used along with
anti inflammatory drugs, which would potentiate the effect of the drugs. © RJPT All
right reserved.
AN - rayyan-553780646
AU - Jain, A.
AU - Anitha, R.
DO - 10.5958/0974-360X.2019.00469.4
IS - 6
KW - Anti inflammatory
Biosynthesis
Cumin oil
UV-vis spectroscopy
cumin oil
silver nanoparticle
unclassified drug
vegetable oil
Article
cumin
drug analysis
drug isolation
drug synthesis
nanomedicine
plant leaf
protein denaturation
ultraviolet visible spectrophotometry
PY - 2019
SP - 2790-2793
ST - Anti inflammatory activity of silver nanoparticles synthesised using cumin
oil
T2 - Research Journal of Pharmacy and Technology
TI - Anti inflammatory activity of silver nanoparticles synthesised using cumin
oil
85074811851&doi=10.5958%2f0974-
360X.2019.00469.4&partnerID=40&md5=6301200f8d69ad313a0812203507cc59
VL - 12
ID - 8670
ER -
TY - JOUR
AB - Antibiotic resistance has necessitated search for new antibacterials for
combating threat of pathogenic bacteria. Though chemically synthesized silver
nanoparticles are a well-known antimicrobial agent, they are toxic to human cells
at higher concentrations. Hence in the present study, curcumin-silver nanoparticles
(Cur–AgNPs) of size 25–35 nm, were synthesized using curcumin, a phytochemical.
These nanoparticles were effective against both Gram positive and Gram-negative
bacteria and were less toxic to human keratinocytes. They had very low total silver
content and high stability. The antibacterial activity of Cur–AgNPs, as studied by
minimum inhibitory concentration (MIC = 5 mg/L), time kill kinetics and post agent
effect, was better than silver nanoparticles (AgNPs, size ≈ 35 nm, MIC = 20 mg/L).
The inhibitory effect of Cur–AgNPs on biofilm formation was also ≈ 20% more than
AgNPs as demonstrated by live–dead imaging and scanning electron microscopy. The
cytotoxic test to skin keratinocytes (HaCaT) showed that Cur–AgNPs were toxic at a
concentration of 156 mg/L which is much higher than the bacterial MIC (selective
toxicity). They also showed anti-inflammatory effect on human macrophages (THP1) by
reducing secretion of pro-inflammatory cytokines IL-6 and TNF-α as compared to
chemically synthesized AgNPs. © 2017, Springer-Verlag GmbH Germany.
AN - rayyan-553780649
AU - Jaiswal, S.
AU - Mishra, P.
DO - 10.1007/s00430-017-0525-y
IS - 1
KW - Antibacterial
Antibiofilm
Curcumin
Macrophage
Minimum inhibitory concentration
Selective toxicity
Silver nanoparticle
Biofilms
Cell Line
Cell Survival
Cytokines
Gram-Negative Bacteria
Gram-Positive Bacteria
Humans
Keratinocytes
Macrophages
Microbial Viability
Nanoparticles
Silver
curcumin
cytokine
interleukin 6
silver nanoparticle
antiinfective agent
nanoparticle
silver
Article
bacterial cell
biofilm
cytokine release
drug cytotoxicity
drug stability
drug synthesis
HaCat cell line
human
human cell
keratinocyte
mammal cell
nonhuman
particle size
priority journal
THP-1 cell line
cell line
cell survival
drug effect
macrophage
microbial viability
physiology
secretion (process)
PY - 2018
SP - 39-53
ST - Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with
improved stability and selective toxicity to bacteria over mammalian cells
T2 - Medical Microbiology and Immunology
TI - Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with
improved stability and selective toxicity to bacteria over mammalian cells
85032390225&doi=10.1007%2fs00430-017-0525-
y&partnerID=40&md5=0753d6b5cf86a30c97f71dfc45318ee9
VL - 207
ID - 8673
ER -
TY - JOUR
AB - Urban air particulate pollution is a known cause for adverse human health
effects worldwide. China has encountered air quality problems in recent years due
to rapid industrialization. Toxicological effects induced by particulate air
pollution vary with particle sizes and season. However, it is not known how
distinctively different photochemical activity and different emission sources
during the day and the night affect the chemical composition of the PM size ranges
and subsequently how it is reflected to the toxicological properties of the PM
exposures. The particulate matter (PM) samples were collected in four different
size ranges (PM10-2.5; PM2.5-1; PM1-0.2 and PM0.2) with a high volume cascade
impactor. The PM samples were extracted with methanol, dried and thereafter used in
the chemical and toxicological analyses. RAW264.7 macrophages were exposed to the
particulate samples in four different doses for 24 h. Cytotoxicity, inflammatory
parameters, cell cycle and genotoxicity were measured after exposure of the cells
to particulate samples. Particles were characterized for their chemical
composition, including ions, element and PAH compounds, and transmission electron
microscopy (TEM) was used to take images of the PM samples. Chemical composition
and the induced toxicological responses of the size segregated PM samples showed
considerable size dependent differences as well as day to night variation. The
PM10-2.5 and the PM0.2 samples had the highest inflammatory potency among the size
ranges. Instead, almost all the PM samples were equally cytotoxic and only minor
differences were seen in genotoxicity and cell cycle effects. Overall, the PM0.2
samples had the highest toxic potential among the different size ranges in many
parameters. PAH compounds in the samples and were generally more abundant during
the night than the day, indicating possible photo-oxidation of the PAH compounds
due to solar radiation. This was reflected to different toxicity in the PM samples.
Some of the day to night difference may have been caused also by differing wind
directions transporting air masses from different emission sources during the day
and the night. The present findings indicate the important role of the local
particle sources and atmospheric processes on the health related toxicological
properties of the PM. The varying toxicological responses evoked by the PM samples
showed the importance of examining various particle sizes. Especially the detected
considerable toxicological activity by PM0.2 size range suggests they're
attributable to combustion sources, new particle formation and atmospheric
processes. © 2015 Elsevier Ltd.
AN - rayyan-553780650
AU - Jalava, P. I.
AU - Wang, Q.
AU - Kuuspalo, K.
AU - Ruusunen, J.
AU - Hao, L.
AU - Fang, D.
AU - Väisänen, O.
AU - Ruuskanen, A.
AU - Sippula, O.
AU - Happo, M. S.
AU - Uski, O.
AU - Kasurinen, S.
AU - Torvela, T.
AU - Koponen, H.
AU - Lehtinen, K. E. J.
AU - Komppula, M.
AU - Gu, C.
AU - Jokiniemi, J.
AU - Hirvonen, M. R.
DO - 10.1016/j.atmosenv.2015.08.089
KW - Air quality
Cytotoxicity
In vitro
Inflammation
Particulate matter
Toxicology
China
Air pollution
Atmospheric chemistry
Chemical analysis
Chemical compounds
Meteorological instruments
Particle size
Pollution
Toxicity
Transmission electron microscopy
aluminum
ammonium nitrate
ammonium sulfate
arsenic
barium ion
cadmium
calcium ion
chromium
copper
ferric ion
iron
lead
magnesium
manganese
metal
molybdenum
nickel
nitrate
potassium
rubidium ion
selenium
silver
sodium
strontium
sulfate
titanium
vanadium
zinc
zinc ion
In-vitro
Particulate air pollution
Particulate Matter
Photochemical activity
Toxicological analysis
Toxicological properties
air mass
air quality
air sampling
atmospheric pollution
genotoxicity
health risk
industrialization
PAH
particle size
particulate matter
phytochemistry
public health
sampler
solar radiation
toxicology
urban atmosphere
wind direction
air pollution
animal cell
Article
cell cycle
controlled study
cytotoxicity
inflammation
macrophage
mouse
nonhuman
photooxidation
priority journal
Polycyclic aromatic hydrocarbons
PY - 2015
SP - 427-437
ST - Day and night variation in chemical composition and toxicological responses
of size segregated urban air PM samples in a high air pollution situation
T2 - Atmospheric Environment
TI - Day and night variation in chemical composition and toxicological responses
of size segregated urban air PM samples in a high air pollution situation
84942013194&doi=10.1016%2fj.atmosenv.2015.08.089&partnerID=40&md5=45397688f45fe0da8
01197076f2ddcb5
VL - 120
ID - 8674
ER -
TY - JOUR
AB - In the current study for the first time, silver nanoparticles (AgNPs) were
biosynthesized by reducing agents from hot water extract of Allium ampeloprasum, an
antibacterial and anti-inflammatory edible plant. UV–vis. spectroscopy, Fourier
transform infrared spectroscopy, dynamic light scattering, X-ray diffractometric,
and transmission electron microscopy (TEM) analyses have been applied to confirm
the formation of biosynthesized AgNPs. Total phenol content and antioxidant
activities of AgNPs and extract together with their antibacterial and cytotoxic
properties, were evaluated. According to TEM, AgNPs were spherical with a diameter
of 8–50 nm. Total phenolic compounds were 15.58 μg/mL, and 10.94 μg/mL at a
concentration of 150 μg/mL for the A. ampeloprasum extract and the biosynthesized
AgNPs, respectively. Biosynthesized AgNPs showed significant antioxidant activity
(81%) as compared to A. ampeloprasum extract (32%) and were active on multi-drug
resistant P. aeruginosa. Besides, the cytotoxic activity response was also
demonstrated that AgNPs were more potent than the A. ampeloprasum extract and
showed high activity against Hela cell line with an IC50 value of less than 25
µg/mL. In conclusion, AgNPs synthesized by A. ampeloprasum extract with excellent
antioxidant and antibacterial effects and acceptable cytotoxicity on cervical
cancer cells have the potential to be used in biological applications. © 2020
Society of Powder Technology Japan
AN - rayyan-553780651
AU - Jalilian, F.
AU - Chahardoli, A.
AU - Sadrjavadi, K.
AU - Fattahi, A.
AU - Shokoohinia, Y.
DO - 10.1016/j.apt.2020.01.011
IS - 3
KW - Allium ampeloprasum
Antibacterial
Antioxidant
Cytotoxicity
Antioxidants
Cell culture
Drug delivery
Light scattering
Metal nanoparticles
Phenols
Reducing agents
Biological applications
Cervical cancer cells
Total phenol contents
Total phenolic compounds
Plants (botany)
PY - 2020
SP - 1323-1332
ST - Green synthesized silver nanoparticle from Allium ampeloprasum aqueous
extract: Characterization, antioxidant activities, antibacterial and cytotoxicity
effects
T2 - Advanced Powder Technology
TI - Green synthesized silver nanoparticle from Allium ampeloprasum aqueous
extract: Characterization, antioxidant activities, antibacterial and cytotoxicity
effects
85078306969&doi=10.1016%2fj.apt.2020.01.011&partnerID=40&md5=f4318fa3775ff98709bdde
dd40c4eddb
VL - 31
ID - 8675
ER -
TY - JOUR
AB - Background There is growing interest in the role of microbes in the
pathogenesis of coronary atherosclerosis but most of the evidence has been
seroepidemiologic. It would be useful to know more about the cytology and histology
of coronary lesions containing clearly depicted microbes. Objective To define
carefully the assorted abnormalities apparent in the coronary arteries of
individuals dying with Whipple's disease. Methods Myocardial tissue from 12 cases
of Whipple's disease was studied by light microscopy. Slides were stained routinely
(in sequence) with either the periodic-acid-Schiff (PAS) or Goldner-trichrome
method and some with Gomori methenimine silver. Cardiac slides with PAS-positive
bacilli were compared to lesions in jejunal lamina propria. Results There were
abundant sites of coronary arterial damage associated with presence of Whipple
bacilli, more in the tunica media than in intima and adventitia. Bacilli in the
arterial lesions were identical to those in lamina propria. Medial lesions were
often associated with a fibroproliferative 'atheroma'. Both intracellular and
extracellular bacilli were found. Most lesions were devoid of inflammation, but
some sites exhibited either florid arteritis or dense scarring. Arteries that were
scarred or inflamed exhibited only a few bacilli. There was an apparent affinity of
bacilli for the nuclei in medial smooth muscle cells and in nearby ventricular
myocytes. Apoptosis (TUNEL-positive) was present in medial smooth muscle cells,
endothelial cells, and ventricular myocytes. Conclusions There is a wide spectrum
of coronary abnormalities in Whipple's disease. It would be useful to know how
often the Whipple bacillus is a part of the total pathogen burden in coronary
disease. Coron Artery Dis 12:115-125 (C) 2001 Lippincott Williams & Wilkins.
AN - rayyan-553780652
AU - James, T. N.
DO - 10.1097/00019501-200103000-00005
IS - 2
PY - 2001
SN - 0954-6928 1473-5830
SP - 115-125
ST - On the wide spectrum of abnormalities in the coronary arteries of Whipple's
disease
T2 - CORONARY ARTERY DISEASE
TI - On the wide spectrum of abnormalities in the coronary arteries of Whipple's
disease
VL - 12
Y2 - 3
ID - 8676
ER -
TY - JOUR
AB - Gold nanoparticles (Au NPs) are used in diagnostic and therapeutic
applications together with a variety of industrial purposes and in many biomedical
sectors with potential risks to human health. The present study aimed to the
histological, histochemical, and ultrastructural alterations induced by Au NPS in
vital organs. Healthy male Wistar Albino rats (Rattus norvegicus) were subjected to
20 injections of 10-nm Au NPs at a daily dose of 2 mg/kg. Liver, kidney, heart, and
lung biopsies from control and Au NPs-treated rats under study were subjected to
histological and histochemical examinations. In comparison with the control rats,
the renal tissue of Au NPs-treated rats demonstrated glomerular congestion,
interstitial inflammatory cell infiltration, renal tubular hydropic degeneration,
cloudy swelling, necrosis, and hyaline cast precipitation. In addition, Au NPs
induced the following hepatic alterations: hepatocyte cytolysis, cytoplasmic
vacuolation, hydropic degeneration, and nuclear alterations together with
sinusoidal dilatation. Moreover, the hearts of the treated rats demonstrated
myocarditis, cardiac congestion, hyalinosis, cardiomyocyte hydropic degeneration,
myofiber disarray and cardiac congestion. The lungs of Au NPs-treated rats also
exhibited the following pulmonary alterations: alectasis, emphysema, inflammatory
cell inflammation, thickened alveolar walls, pulmonary interstitial edema,
congestion, hypersensitivity, fibrocyte proliferation, and honeycombing. In
conclusion, exposure to Au NPs induced histological, histochemical and
ultrastructural alterations in the vital organs that may alter the function of
these organs. Additional efforts are needed for better understanding the potential
risks of Au NPs to human health.
AN - rayyan-553780655
AU - Jarrar, Q.
AU - Al-Doaiss, A.
AU - Jarrar, B. M.
AU - Alshehri, M.
DO - 10.1177/07482337221133881
IS - 12
PY - 2022
SN - 0748-2337 1477-0393
SP - 789-800
ST - On the toxicity of gold nanoparticles: Histological, histochemical and
ultrastructural alterations
T2 - TOXICOLOGY AND INDUSTRIAL HEALTH
TI - On the toxicity of gold nanoparticles: Histological, histochemical and
ultrastructural alterations
VL - 38
Y2 - 12
ID - 8679
ER -
TY - JOUR
AB - Metallic nanoparticles (NPs) are widely used in medical preparations. The
present study aims to find out the influence of widely used five metallic NPs on
the expression of major hepatic drug-metabolizing enzyme (DME) genes. Six groups of
BALB/C mice, 7 mice each, were exposed to: Gold NPs, silver NPs, copper oxide NPs,
silicon dioxide NPs and zinc oxide NPs, for 21 days. Liver biopsies from all mice
were subjected to mouse cyp3a11, cyp2c29, ugt2b1 and interleukin-6 (il6) gene
expression quantification using real-time polymerase chain reaction, in addition to
inflammatory cell infiltration examination. All tested NPs caused a sharp and
significant (ANOVA, p value <0.05) downregulation in the expression of DME genes,
with the highest influence was observed in mice exposed to copper oxide NPs.
Additionally, all NPs induced hepatic inflammation and upregulated the expression
of il6 gene, which were inversely correlated with the expression of DMEs. It is
concluded that all tested NPs downregulated the expression of DME genes, with the
highest influence exhibited by copper oxide NPs, in correlation with inflammation
and il6 gene induction in the liver. Further studies are needed to find out the
effect of anti-inflammatory compounds against the alterations induced by metallic
NPs exposure on hepatic DMEs. © 2020 Elsevier B.V.
AN - rayyan-553780656
AU - Jarrar, Y.
AU - Al-Doaiss, A.
AU - Alfaifi, M.
AU - Shati, A.
AU - Al-Kahtani, M.
AU - Jarrar, B.
DO - 10.1016/j.etap.2020.103449
KW - Drug metabolizing enzymes
Gene expression
Interleukin 6
Liver
Nanobiology
Nanoparticles
Animals
Cytochrome P-450 CYP2C9
Cytochrome P-450 CYP3A
Down-Regulation
Environmental Pollutants
Gene Expression
Inflammation
Interleukin-6
Male
Metabolic Clearance Rate
Metal Nanoparticles
Mice, Inbred BALB C
cytochrome P450 3A11
drug metabolizing enzyme
gold nanoparticle
interleukin 6
metal nanoparticle
silica nanoparticle
silver nanoparticle
cytochrome P450 2C9
cytochrome P450 3A
cytochrome P450 3A4, mouse
interleukin-6, mouse
adult
animal cell
animal experiment
animal tissue
Article
Bagg albino mouse
cell infiltration
controlled study
cyp2c29 gene
cyp3a11 gene
down regulation
exposure
gene expression
hepatitis
il6 gene
inflammatory cell
liver biopsy
male
mouse
nonhuman
priority journal
toxicity
ugt2b1 gene
upregulation
animal
drug effect
enzymology
genetics
immunology
inflammation
liver
metabolic clearance rate
pathology
pharmacokinetics
pollutant
PY - 2020
ST - The influence of five metallic nanoparticles on the expression of major drug-
metabolizing enzyme genes with correlation of inflammation in mouse livers
T2 - Environmental Toxicology and Pharmacology
TI - The influence of five metallic nanoparticles on the expression of major drug-
metabolizing enzyme genes with correlation of inflammation in mouse livers
85086942302&doi=10.1016%2fj.etap.2020.103449&partnerID=40&md5=954d47809e76f5690cdaa
88486bf8fa5
VL - 80
ID - 8680
ER -
TY - JOUR
AB - Biosynthesis of zinc oxide nanoparticles (ZnO-NPs) was achieved by utilizing
the reducing and capping potential of leaf, stem and callus aqueous extracts of
Mussaenda frondosa.The bioreduced ZnO-NPs were characterized using powder X-ray
diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis spectroscopy), scanning
electron microscopy (SEM), energy dispersive spectroscopy (EDS), fourier transform
infrared spectroscopy (FTIR) and dynamic light scattering (DLS) techniques. UV-
visible spectra of ZnO-NPs showed a strong absorption peak at 370, 376 and 373 nm
corresponding to the band gap energy of 3.33, 3.27 and 3.30 eV for ZnO-NPs obtained
from leaf (L-ZnO-NP), stem (S-ZnO-NP) and callus (C-ZnO-NP) aqueous extracts,
respectively. XRD analysis confirmed the formation of hexagonal wurtzite structures
having an average grain size between 5 and 20 nm in diameter. FTIR spectra revealed
the presence of stretching vibrations of -O-H, C-H, C-N, C = O groups involved in
reduction and stabilization of nanoparticles. SEM images recognize the presence of
spongy, spherical, porous agglomerated nanoparticles. DLS analysis and zeta
potential values validated the stability of ZnO-NPs. The present investigation puts
light on the photocatalytic activity and biological (antioxidant, anti-
inflammatory, antidiabetic, antimicrobial, anticancerous) applications of ZnO-NPs.
The current study is an attempt to describe an effective, simple and eco-friendly
method of ZnO-NP synthesis and to evaluate its potential for various industrial and
medical applications.
AN - rayyan-553780657
AU - Jayappa, M. D.
AU - Ramaiah, C. K.
AU - Kumar, M. A. P.
AU - Suresh, D.
AU - Prabhu, A.
AU - Devasya, R. P.
AU - Sheikh, S.
DO - 10.1007/s13204-020-01382-2
IS - 8
KW - Zinc
PY - 2020
SN - 2190-5509 2190-5517
SP - 3057-3074
ST - Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro
grown callus of Mussaenda frondosa L.: characterization and their applications
T2 - APPLIED NANOSCIENCE
TI - Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro
grown callus of Mussaenda frondosa L.: characterization and their applications
VL - 10
Y2 - 8
ID - 8681
ER -
TY - JOUR
AB - Background: The field of medicine and synthetic drug development have
advanced rapidly over the past few decades. However, research on alternative
medicine, such as phytochemicals cannot be ignored. The main reason for prominent
curiosity about phytochemicals stems from the belief that the usage of natural
compounds are safer and have lesser detrimental side effects. Objective: The aim of
the present review was to discuss in detail several phytochemicals that have been
studied or are being studied in the context of various neurological disorders,
including depression, Alzheimer’s disease, Huntington’s disease and even
neuroinflammatory disorders, such as encephalitis. Methods: The potential roles of
phytochemicals in treating or managing symptoms associated with neurological
disorders have been included in this article. All data included in this paper have
been pooled from various databases, including Google Scholar, PubMed, Science
Direct, Springer, and Wiley Online Library. Results: Phytochemicals have been
widely studied for their therapeutic properties associated with neurological
disorders. Using various experimental techniques for both in vivo and in vitro
experiments, studies have shown that phytochemicals do have antioxidant, anti-
inflammatory and neuroprotective activities, which play major roles in the
treatment of neurological diseases. Conclusion: Even though there has been
compelling evidence of the therapeutic role of phytochemicals, further research is
still required to evaluate the safety and efficacy of these medicines. Using
previously published papers as the foundation for additional research, such as
preclinical studies and clinical trials, phytochemicals can become a safer
alternative to synthetic drugs for treating a spectrum of neurological diseases. ©
2023, Bentham Science Publishers. All rights reserved.
AN - rayyan-553780658
AU - Jayaraman, M.
AU - Dutta, P.
AU - Krishnan, S.
AU - Arora, K.
AU - Sivakumar, D.
AU - Raghavendran, H. R. B.
DO - 10.2174/1871527321666220701153926
IS - 9
KW - alternative medicine
cancer
medicinal plants
neuro-inflammatory disorders
neuro-psychiatric
neurological
Phytochemicals
alkaloid
amine oxidase (flavin containing) isoenzyme A
antioxidant
apigenin
ataxin 3
berberine
betulic acid
borneol
brain derived neurotrophic factor
calcitonin gene related peptide
camphor
cannabidiol
carotenoid
carvacrol
catalase
catechin
chalcone
citalopram
cocaine
corticosteroid
corticosterone
corticotropin
crocin
curcumin
cyclooxygenase 2
deutetrabenazine
diosgenin
dopamine
endothelin 1
Fas ligand
ferulic acid
flavonoid
fluoxetine
fluticasone propionate
furocoumarin
galanin
gallic acid
gibberellin
glutathione
herbaceous agent
interleukin 17
jatrorrhizine
lactulose
licochalcone A
liposome
luteolin
lycopene
magnolol
malathion
mangiferin
mitochondrial DNA
myelin oligodendrocyte glycoprotein
natural product
neuropeptide Y
noradrenalin
oleanolic acid
oxindole
palmatine
parkin
parthenolide
pentetrazole
phenytoin
phytochemical
phytol
phytosterol
piperine
plant extract
polyglutamine
polyphenol
prion protein
probenecid
prostaglandin E2
pyridine derivative
quercetin
quetiapine
resveratrol
risperidone
ropinirole
saponin
sesquiterpene
silver nanoparticle
stigmasterol
survival motor neuron protein
tachykinin
terpene
tetrabenazine
thymol
thymoquinone
transcription factor FOXP3
triptolide
ursolic acid
valproic acid
virulence factor
Acanthamoeba castellanii
Achillea
Acinetobacter baumannii
aconite
allopathy
Alphavirus
Alzheimer disease
amygdala
amyotrophic lateral sclerosis
Andrographis paniculata
anticonvulsant activity
antidepressant activity
anxiety
apoptosis
aromatherapy
Artemisia capillaris
Asteraceae
astrocyte
astrocytosis
ataxia
Bacopa monnieri
bacterium adherence
bioinformatics
black cumin
blood brain barrier
bradykinesia
brain depth stimulation
brain ischemia
Bupleurum
Caenorhabditis elegans
callus
cancer prevention
Candida albicans
Cannabis sativa
cardiovascular disease
cell infiltration
cell proliferation
Centella asiatica
chorea
coculture
cognition
cognitive defect
Coptis chinensis
coriander
Crocus sativus
cytokine production
cytotoxicity
demyelinating disease
demyelination
depression
discriminant analysis
DNA damage
drug design
drug safety
electric shock
encephalitis
epigenetics
Escherichia coli
Euterpe oleracea
Fabaceae
fatigue
flower color
forced swim test
gene expression
genetic counseling
genotype
Ginkgo biloba
ginseng
gooseberry
Haemophilus influenzae
headache
hemiparesis
Heracleum
herbal medicine
hippocampus
human
Huntington chorea
Hypericaceae
hyperlipidemia
hypothalamus hypophysis adrenal system
hypoxia
in vitro study
insect vector
intellectual impairment
Lantana camara
lipid peroxidation
Listeria monocytogenes
liver microsome
locomotion
Magnolia officinalis
major depression
malignant neoplasm
medicinal plant
meningoencephalitis
mental disease
microglia
migraine
mitochondrial biogenesis
molecular docking
Moringa oleifera
multiple myeloma
multiple sclerosis
muscle atrophy
myelination
myocarditis
Neisseria meningitidis
nerve degeneration
nervousness
neurofibrillary tangle
neurologic disease
neuroprotection
neurotoxicity
nonhuman
Ocimum tenuiflorum
oligodendroglia
optic neuritis
orchitis
oxidative stress
paresthesia
Parkinson disease
parkinsonism
parotitis
Passiflora
phagocytosis
phonophobia
Phyllanthus emblica
phytochemistry
posttraumatic stress disorder
prevalence
Primula
prion disease
protein aggregation
protein misfolding
quality of life
regulatory T lymphocyte
remyelinization
Review
risk assessment
rosemary
schizophrenia
seizure
signal transduction
Solanum nigrum
spinal muscular atrophy
spinocerebellar degeneration
splicing defect
Streptococcus pneumoniae
Terminalia bellirica
Tinospora cordifolia
traumatic brain injury
trinucleotide repeat
ultra performance liquid chromatography
upregulation
Veratrum
virus replication
watermelon
Withania somnifera
Wnt signaling
wound healing
zebra fish
Nervous System Diseases
PY - 2023
SP - 1275-1301
ST - Emerging Promise of Phytochemicals in Ameliorating Neurological Disorders
T2 - CNS and Neurological Disorders - Drug Targets
TI - Emerging Promise of Phytochemicals in Ameliorating Neurological Disorders
85166393840&doi=10.2174%2f1871527321666220701153926&partnerID=40&md5=3a19c38a334210
7505b45ebea31b5049
VL - 22
ID - 8682
ER -
TY - JOUR
AB - Inhalation of engineered nanoparticles (NP) poses a still unknown risk.
Individuals with chronic lung diseases are expected to be more vulnerable to
adverse effects of NP than normal subjects, due to altered respiratory structures
and functions. Realistic and dose-controlled aerosol exposures were performed using
the deposition chamber NACIVT. Well-differentiated normal and cystic fibrosis (CF)
human bronchial epithelia (HBE) with established air-liquid interface and the human
bronchial epithelial cell line BEAS-2B were exposed to spark-generated silver and
carbon nanoaerosols (20 nm diameter) at three different doses. Necrotic and
apoptotic cell death, pro-inflammatory response, epithelial function and morphology
were assessed within 24 h after aerosol exposure. NP exposure resulted in
significantly higher necrosis in CF than normal HBE and BEAS-2B cells. Before and
after NP treatment, CF HBE had higher caspase-3 activity and secreted more IL-6 and
MCP-1 than normal HBE. Differentiated HBE had higher baseline secretion of IL-8 and
less caspase-3 activity and MCP-1 secretion compared to BEAS-2B cells. These
biomarkers increased moderately in response to NP exposure, except for MCP-1, which
was reduced in HBE after AgNP treatment. No functional and structural alterations
of the epithelia were observed in response to NP exposure. Significant differences
between cell models suggest that more than one and fully differentiated HBE should
be used in future toxicity studies of NP in vitro.Our findings support
epidemiologic evidence that subjects with chronic airway diseases are more
vulnerable to adverse effects of particulate air pollution. Thus, this sub-
population needs to be included in nano-toxicity studies. © 2015 Informa UK Ltd.
All rights reserved: reproduction in whole or part not permitted.
AN - rayyan-553780662
AU - Jeannet, N.
AU - Fierz, M.
AU - Schneider, S.
AU - Künzi, L.
AU - Baumlin, N.
AU - Salathe, M.
AU - Burtscher, H.
AU - Geiser, M.
DO - 10.3109/17435390.2015.1049233
IS - 3
KW - Air'liquid interface
airways
cell death
NACIVT
pro-inflammatory cytokines
Aerosols
Carbon
Cell Death
Cell Proliferation
Cells, Cultured
Cystic Fibrosis
Epithelial Cells
Humans
Nanoparticles
Particulate Matter
Respiratory Mucosa
Silver
carbon nanoparticle
caspase 3
interleukin 6
interleukin 8
silver nanoparticle
aerosol
autacoid
carbon
nanoparticle
particulate matter
silver
acute toxicity
air pollution
Article
cell culture
ciliated epithelium
dose response
enzyme activity
epithelial lining fluid
human
human cell
lung fibrosis
neutrophil
nonhuman
priority journal
respiratory epithelium
cell proliferation
chemistry
cystic fibrosis
cytology
drug effects
epithelium cell
metabolism
pathology
physiology
respiratory mucosa
toxicity
Humanities
Humanism
PY - 2016
SP - 279-291
ST - Acute toxicity of silver and carbon nanoaerosols to normal and cystic
fibrosis human bronchial epithelial cells
T2 - Nanotoxicology
TI - Acute toxicity of silver and carbon nanoaerosols to normal and cystic
fibrosis human bronchial epithelial cells
84959268343&doi=10.3109%2f17435390.2015.1049233&partnerID=40&md5=0f76fc6b74d89bbbfc
844637fcd58dd6
VL - 10
ID - 8686
ER -
TY - JOUR
AB - An ongoing need for new cancer therapeutics exists, especially ones that
specifically home and target triple-negative breast cancer. Because triple-negative
breast cancer express low or are devoid of estrogen, progesterone, or Her2/Neu
receptors, another target must be used for advanced drug delivery strategies. Here,
we engineered a nanodrug delivery system consisting of silver-coated gold nanorods
(AuNR/Ag) targeting epithelial cell adhesion/activating molecule (EpCAM) and loaded
with doxorubicin. This nanodrug system, AuNR/Ag/Dox-EpCAM, was found to
specifically target EpCAM-expressing tumors compared to low EpCAM-expressing
tumors. Namely, the nanodrug had an effective dose (ED50) of 3 mu M in inhibiting
4T1 cell viability and an ED50 of 110 mu M for MDA-MD-231 cells. Flow cytometry
data indicated that 4T1 cells, on average, express two orders of magnitude more
EpCAM than MDA-MD-231 cells, which correlates with our ED50 findings. Moreover, due
to the silver coating, the AuNR/Ag can be detected simultaneously by surface-
enhanced Raman spectroscopy and photoacoustic microscopy. Analysis by these imaging
detection techniques as well as by inductively coupled plasma mass spectrometry
showed that the targeted nanodrug system was taken up by EpCAM-expressing cells and
tumors at significantly higher rates than untargeted nanoparticles (p < 0.05).
Thus, this approach establishes a plasmonically active nanodrug theranostic for
triple-negative breast cancer and, potentially, a delivery platform with improved
multimodal imaging capability for other clinically relevant chemotherapeutics with
dose-limiting toxicities, such as platinum-based or taxane-based therapies.
AN - rayyan-553780664
AU - Jenkins, S. V.
AU - Nima, Z. A.
AU - Vang, K. B.
AU - Kannarpady, G.
AU - Nedosekin, D. A.
AU - Zharov, V. P.
AU - Griffin, R. J.
AU - Biris, A. S.
AU - Dings, R. P. M.
DO - 10.1038/s41698-017-0030-1
KW - Breast Neoplasms
Triple Negative Breast Neoplasms
PY - 2017
SN - 2397-768X
ST - Triple-negative breast cancer targeting and killing by EpCAM-directed,
plasmonically active nanodrug systems
T2 - NPJ PRECISION ONCOLOGY
TI - Triple-negative breast cancer targeting and killing by EpCAM-directed,
plasmonically active nanodrug systems
VL - 1
Y2 - 9 y3 - 1
ID - 8687
ER -
TY - JOUR
AB - Since its first use in 1917, sulphur mustard (SM) has been used virtually
exclusively as a weapon of war. SM is a volatile liquid that damages any tissue it
contacts as a vapour or liquid. SM primarily damages the skin, eyes and lungs
producing massive inflammation culminating in the characteristic blistering of the
skin which classifies SM as a vesicant. Several mechanisms of action at the
cellular level have been proposed for SM, but none has ever been convincingly
linked to the production of blisters or vesication. First aid for those
contaminated with liquid SM consists of the rapid removal (within a few minutes) of
liquid from the surface of the skin, as once penetrated into the stratum corneum it
is very difficult to remove. In the absence of a mechanistically based specific
therapy, SM skin injury is normally treated in a similar way to thermal and
chemical burns, which it resembles pathologically. Effective therapy consist of
treating the inflammation and where necessary removal of the dead eschar to
facilitate healing. Post surgical care comprises the use of one of a number of
available dressings used in thermal burn care and antibiotic creams should
infection be present. © 2013 Elsevier B.V.
AN - rayyan-553780665
AU - Jenner, J.
AU - Graham, S. J.
DO - 10.1016/j.cbi.2013.10.015
IS - 3
KW - Adjunct therapies
Anti-inflammatories
Chemical weapons
Dressings
Sulphur mustard
Surgical treatment
Animals
Bandages
Chemical Warfare Agents
Erythema
Humans
Mustard Gas
Skin
Skin Diseases
acetylcysteine
chlorpromazine
dexamethasone
diclofenac
dimercaprol
doxycycline
hydrocortisone
indometacin
methylprednisolone
mustard gas
nicotinamide
octyl homovanillamide
olvanil
proteinase inhibitor
retro olvanil
silibinin
unclassified drug
adhesive dressing
alginate dressing
article
cytotoxicity
debridement
erbium YAG laser
foam dressing
human
hydrocolloid dressing
hydrogel dressing
nonhuman
pathology
silver donating dressing
skin decontamination
skin incision
skin injury
skin surgery
supplementation
vacuum assisted closure
wound dressing
wound healing
PY - 2013
SP - 491-495
ST - Treatment of sulphur mustard skin injury
TI - Treatment of sulphur mustard skin injury
84890127101&doi=10.1016%2fj.cbi.2013.10.015&partnerID=40&md5=de15eb3bef35b60833640b
59bf6207e5
VL - 206
ID - 8688
ER -
TY - JOUR
AB - The aim of the present study was to systematically review studies
investigating antibacterial implant abutment surfaces or coatings, which may
suppress bacterial growth to prevent plaque-induced peri-implant inflammatory
disease. Data were collected after identification of case, assay/laboratory
procedure, predicate/reference standard and outcome (CAPO). Seven hundred and
twenty (720) records were identified through data base searching. After screening
nine publications fulfilled inclusion criteria and were included. The following
surfaces/coatings showed antibacterial properties: Electrochemical surface
modification of titanium by the anodic spark deposition tech-nique; doxycycline
coating by cathodic polarization; silver coating by DC plasma sputter; titanium
nitride; zirconium nitride and microwave assistant nano silver coating. Since the
current state of the literature is rather descriptive, a meta-analysis was not
performed. While several abutment coatings showed to have antibacterial capacity,
some of them also influenced the behavior of investigated human cells. None of the
studies investigated the long-term effect of surface modifications. Since surface
changes are the main contributing factor in the development of antibacterial
effects, the biodegradation behavior must be characterized to understand its
durability. To date there is no effective structure, material or strategy to avoid
periimplant inflammation used as clinical routine. Furthermore, clinical studies
are scarce. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780666
AU - Jennes, M. E.
AU - Naumann, M.
AU - Peroz, S.
AU - Beuer, F.
AU - Schmidt, F.
IS - 11
KW - Abutment
Antibacterial coating
Implant
Peri-implantitis
Prevention
silver nanoparticle
Article
bacterial viability
biocompatibility
bone mineralization
bone regeneration
cytotoxicity
dispersity
electrochemical analysis
electrospinning
fracture healing
inflammation
intestine metaplasia
macrophage
MTT assay
nonhuman
osseointegration
periimplantitis
periodontitis
phase contrast microscopy
Streptococcus mutans
Streptococcus salivarius
surface property
systematic review
tooth disease
tooth plaque
PY - 2021
ST - Antibacterial effects of modified implant abutment surfaces for the
prevention of peri-implantitis—a systematic review
T2 - Antibiotics
TI - Antibacterial effects of modified implant abutment surfaces for the
prevention of peri-implantitis—a systematic review
85118947561&doi=10.3390%2fantibiotics10111350&partnerID=40&md5=e8d9c7b94a70ecf1bcaa
3838ed9ca8e9
VL - 10
ID - 8689
ER -
TY - JOUR
AB - The measurement of nanoparticles (NPs) in a biological matrix is essential in
various toxicity studies. However, the current knowledge has limitations in
differentiating particulate and ionic forms and further identification of their
biotransformation. Herein, we evaluate the biotransformation and differential lung
clearance kinetics of particulate and ionic forms using PEGylated silver NPs (AgNP-
PEGs; 47.51 nm) and PEGylated gold NPs (AuNP-PEGs; 11.76 nm). At 0, 3, and 6 h and
1, 3, 7, and 14 days after a single pharyngeal aspiration in mice at 25 μg/mouse,
half of the lung is digested by proteinase K (PK) to separate particulates and
ions, and the other half is subjected to the acid digestion method for comparison.
The quantitative and qualitative evaluation of lung clearance kinetics suggests
that AgNP-PEGs are quickly dissolved and transformed into insoluble silver sulfide
(Ag2S), which shows a fast-clearing early phase (0 −6 h; particle T1/2: 4.8 h) and
slow-clearing late phase (1 −14 days; particle T1/2: 13.20 days). In contrast,
AuNP-PEGs were scarcely cleared or biotransformed in the lungs for 14 days. The
lung clearance kinetics of AgNPs and biotransformation shown in this study can be
informed by the PK digestion method and cannot be obtained using the acid digestion
method. © 2023 Elsevier B.V.
AN - rayyan-553780667
AU - Jeon, S.
AU - Lee, W. S.
AU - Song, K. S.
AU - Jeong, J.
AU - Lee, S.
AU - Kim, S.
AU - Kim, G.
AU - Kim, J. S.
AU - Cho, W. S.
DO - 10.1016/j.jhazmat.2023.131223
KW - Dissolution
Inhalation
Lung burden
Particokinetics
Toxicokinetics
Animals
Biotransformation
Ions
Lung
Metal Nanoparticles
Mice
Particle Size
Silver
Bioconversion
Biological organs
Kinetics
Particles (particulate matter)
Silver compounds
Sulfur compounds
dissolved oxygen
ion
isoflurane
proteinase K
silver nanoparticle
silver sulfide
sulfide
unclassified drug
macrogol
metal nanoparticle
silver
Acid digestion methods
Ionic forms
Particle kinetics
Particokinetic
Particulates
Pegylated
Proteinase K
acid digestion
biotransformation
nanoparticle
reaction kinetics
toxicity
animal experiment
animal model
animal tissue
Article
blood vessel permeability
comparative study
controlled study
dosimetry
female
hydrodynamics
inductively coupled plasma mass spectrometry
inflammatory cell
innate immunity
lung burden
lung clearance
lung parenchyma
macrophage
morphological trait
mouse
neutrophil
nonhuman
particle size
pathogenesis
PEGylation
phagolysosome
pneumonia
animal
lung
metabolism
Mammals
Sulfides
PY - 2023
ST - Differential particle and ion kinetics of silver nanoparticles in the lungs
and biotransformation to insoluble silver sulfide
T2 - Journal of Hazardous Materials
TI - Differential particle and ion kinetics of silver nanoparticles in the lungs
and biotransformation to insoluble silver sulfide
85150437173&doi=10.1016%2fj.jhazmat.2023.131223&partnerID=40&md5=a575d0371db1cffc38
b978b39e32a9a7
VL - 452
ID - 8690
ER -
TY - JOUR
AB - The increased interest in nanomedicine and its applicability for a wide range
of biological functions demands the search for raw materials to create
nanomaterials. Recent trends have focused on the use of green chemistry to
synthesize metal and metal-oxide nanoparticles. Bioactive chemicals have been found
in a variety of marine organisms, including invertebrates, marine mammals, fish,
algae, plankton, fungi, and bacteria. These marine-derived active chemicals have
been widely used for various biological properties. Marine-derived materials,
either whole extracts or pure components, are employed in the synthesis of
nanoparticles due to their ease of availability, low cost of production,
biocompatibility, and low cytotoxicity toward eukaryotic cells. These marine-
derived nanomaterials have been employed to treat infectious diseases caused by
bacteria, fungi, and viruses as well as treat non-infectious diseases, such as
tumors, cancer, inflammatory responses, and diabetes, and support wound healing.
Furthermore, several polymeric materials derived from the marine, such as chitosan
and alginate, are exploited as nanocarriers in drug delivery. Moreover, a variety
of pure bioactive compounds have been loaded onto polymeric nanocarriers and
employed to treat infectious and non-infectious diseases. The current review is
focused on a thorough overview of nanoparticle synthesis and its biological
applications made from their entire extracts or pure chemicals derived from marine
sources.
AN - rayyan-553780668
AU - Jeong, G. J.
AU - Khan, S.
AU - Tabassum, N.
AU - Khan, F.
AU - Kim, Y. M.
DO - 10.3390/md20080527
IS - 8
KW - Drug Synergism
Military Personnel
PY - 2022
SN - 1660-3397
ST - Marine-Bioinspired Nanoparticles as Potential Drugs for Multiple Biological
Roles
T2 - MARINE DRUGS
TI - Marine-Bioinspired Nanoparticles as Potential Drugs for Multiple Biological
Roles
VL - 20
Y2 - 8
ID - 8691
ER -
TY - JOUR
AB - Overexpression of pro-inflammatory cytokines, including tumour necrosis
factor alpha (TNF alpha), has been implicated in the pathogenesis of anaemia of
inflammation. TNF alpha suppresses erythroid colony formation via both direct and
indirect effects on haematopoietic progenitors, often involving activation of
nuclear factor (NF-kappa B signalling resulting in downregulation of transcription
factors critical for erythropoiesis. There is a dearth of effective and safe
therapies for many patients with inflammatory anaemia. Resveratrol is a flavanol
found in red wine grapes that possesses potent anti-inflammatory properties, but
studies of its impact on human erythropoiesis have proven contradictory. We
investigated whether resveratrol ameliorates TNF alpha-mediated suppression of
erythropoiesis in human CD34(+) haematopoietic progenitors. We found that
resveratrol partially reverses the erythroid suppressive effects of TNF alpha,
leading to significant recovery in burst forming unit-erythroid colony formation in
human CD34(+) cells. CD34(+) cells preincubated with resveratrol for 72 h in the
presence of TNF alpha inhibited NF-kappa B activation via decreased NF-kappa B
nuclear localization without altering total NF-kappa B protein levels and
independent of I kappa B degradation. Resveratrol also significantly restored the
baseline expression of erythroid transcription factors NFE2 and the GATA1/GATA2
ratio in CD34(+) cells treated with TNF alpha. In conclusion, resveratrol may
inhibit TNF alpha-mediated NF-kappa B activation and promote erythropoiesis in
primary human CD34(+) cells.
AN - rayyan-553780669
AU - Jeong, J. Y.
AU - Silver, M.
AU - Parnes, A.
AU - Nikiforow, S.
AU - Berliner, N.
AU - Vanasse, G. J.
DO - 10.1111/j.1365-2141.2011.08800.x
IS - 1
KW - Humanities
Humanism
Humans
PY - 2011
SN - 0007-1048
SP - 93-101
ST - Resveratrol ameliorates TNF alpha-mediated suppression of erythropoiesis in
human CD34(+) cells via modulation of NF-kappa B signalling
T2 - BRITISH JOURNAL OF HAEMATOLOGY
TI - Resveratrol ameliorates TNF alpha-mediated suppression of erythropoiesis in
human CD34(+) cells via modulation of NF-kappa B signalling
VL - 155
Y2 - 10
ID - 8692
ER -
TY - JOUR
AB - ObjectiveNeurodegeneration induced by inflammatory stress in multiple
sclerosis (MS) leads to long-term neurological disabilities that are not amenable
to current immunomodulatory therapies. Methods and ResultsHere, we report that
neuronal downregulation of Splicing factor 3b subunit 2 (SF3B2), a component of U2
small nuclear ribonucleoprotein (snRNP), preserves retinal ganglion cell (RGC)
survival and axonal integrity in experimental autoimmune encephalomyelitis (EAE)-
induced mice. By employing an in vitro system recapitulating the inflammatory
environment of MS lesion, we show that when SF3B2 levels are downregulated, cell
viability and axon integrity are preserved in cortical neurons against inflammatory
toxicity. Notably, knockdown of SF3B2 suppresses the expression of injury-response
and necroptosis genes and prevents activation of Sterile Alpha and TIR Motif
Containing 1 (Sarm1), a key enzyme that mediates programmed axon degeneration.
InterpretationTogether, these findings suggest that the downregulation of SF3B2 is
a novel potential therapeutic target to prevent secondary neurodegeneration in MS.
AN - rayyan-553780670
AU - Jeong, Y. E.
AU - Rajbhandari, L.
AU - Kim, B. W.
AU - Venkatesan, A.
AU - Hoke, A.
DO - 10.1002/acn3.51717
IS - 2
KW - Multiple Sclerosis
PY - 2023
SN - 2328-9503
SP - 246-265
ST - Downregulation of SF3B2 protects CNS neurons in models of multiple sclerosis
T2 - ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
TI - Downregulation of SF3B2 protects CNS neurons in models of multiple sclerosis
VL - 10
Y2 - 2
ID - 8693
ER -
TY - JOUR
AB - Cratoxylum formosum Dyer is a medicinal plant widely found in Asia and
commonly consumed for food and folk medicine. It is rich in phenolic compounds. The
present study utilized water crude extract of C. formosum leaves to synthesize zinc
oxide nanoparticles (ZnO NPs) by green synthesis. The synthesized ZnO NPs with the
average electronic band gap similar to 3 eV were obtained and found to either have
spherical shape or sheet-like structures depending on synthesis process and
concentration of crude extract. Higher concentration of C. formosum extract also
eliminates impurity of Zn(OH)(2) during the synthesis. Results from an agar disk
diffusion assay demonstrated that all synthesized ZnO samples inhibited growth of
Gram-positive bacteria, Bacillus subtilis and Staphylococcus epidermidis and Gram-
negative bacterium, Escherichia coli. Furthermore, all synthesized ZnO demonstrated
potent anti-cancer activity against non-melanoma skin cancer cells (A431) and the
intermediary of cancerous keratinocytes (HaCaT) without affecting normal cell lines
(Vero). In addition, we observed that the ZnO nanosheet offered stronger
cytotoxicity effects against A431 than spherical shaped ZnO particles. Analysis of
RNA-sequencing data revealed that synthesized ZnO nanosheets altered the number of
genes in pathways involved in cancer and MAPK signaling pathways in A431 cells.
Several isoforms of metallothionein transcripts were upregulated including
transcripts involved in inflammatory responses whereas transcripts promoted cell
proliferation and apoptosis were downregulated. Therefore, these studies firstly
reported potential usage of the green-synthesized ZnO nanosheets from C. formosum
extract for development of antibacterial substances or anticancer drugs.
AN - rayyan-553780671
AU - Jevapatarakul, D.
AU - T-Thienprasert, J.
AU - Payungporn, S.
AU - Chavalit, T.
AU - Khamwut, A.
AU - T-Thienprasert, N. P.
DO - 10.1016/j.biopha.2020.110552
KW - Skin Neoplasms
Gene Expression
PY - 2020
SN - 0753-3322 1950-6007
ST - Utilization of Cratoxylum formosum crude extract for synthesis of ZnO
nanosheets: Characterization, biological activities and effects on gene expression
of nonmelanoma skin cancer cell
T2 - BIOMEDICINE & PHARMACOTHERAPY
TI - Utilization of Cratoxylum formosum crude extract for synthesis of ZnO
nanosheets: Characterization, biological activities and effects on gene expression
of nonmelanoma skin cancer cell
VL - 130
Y2 - 10
ID - 8694
ER -
TY - JOUR
AB - Disease-associated nucleic acids, such as DNAs and miRNAs, are important
biomarkers for the diagnosis, prognosis and treatment guidance of human diseases.
Therefore, the accurate and sensitive detection of nucleic acid is of great
significance for the early diagnosis of diseases. DNA-scaffolded silver nanocluster
(DNA-Ag NC) is a new type of probe with good photostability and low toxicity that
has been widely used in biomedical analysis. In this work, a new universal sensing
platform based on target triggered labeling luminescent DNA-Ag NC for disease-
related nucleic acids detection was constructed. The assembled split DNA fragment
pair (C4AC4T and C3GT4) could be used as a template to develop a bright green
fluorescent Ag NC. According to this phenomenon, we devised two probe sequences DNA
1 and DNA 2, which could hybridize to the same one target and contained a different
split fragment of Ag NC’ scaffold. The target compelled the split fragments close
to each other through base pairing with DNA 1 and DNA 2, thus quantification of the
target could be achieved through measuring green fluorescence of Ag NC that
produced by assembled scaffold in ternary hybrid products. We applied this platform
successfully for miR-362, a potential biomarker of inflammatory bowel diseases
(IBD), or HIV-related DNA (hDNA) detection, achieving the detection limits of 6.5
nM and 1.7 nM, respectively. Both of the assays showed excellent reproducibility,
selectivity and potential applications in human serum samples. In summary, an
economic and convenient universal platform was developed for disease-associated
nucleic acid detection. © 2021 Elsevier B.V.
AN - rayyan-553780677
AU - Jia, Z.
AU - Tu, K.
AU - Xu, Q.
AU - Gao, W.
AU - Liu, C.
AU - Fang, B.
AU - Zhang, M.
DO - 10.1016/j.aca.2021.338734
KW - Biosensor
DNA-sliver nanocluster
Fluorescence probe
Nucleic acid
Biosensing Techniques
DNA
Humans
Limit of Detection
Metal Nanoparticles
Nucleic Acids
Reproducibility of Results
Silver
Biomarkers
Biomolecules
Diagnosis
Diseases
DNA sequences
Fluorescence
Nanoclusters
Probes
Scaffolds
biological marker
DNA fragment
fluorescent dye
gold nanoparticle
graphene oxide
green fluorescent protein
microRNA
microRNA 141
microRNA 21
microRNA 223
microRNA 362
nanomaterial
nucleic acid
oligonucleotide
unclassified drug
metal nanoparticle
silver
Ag$++$
Diagnoses of disease
Early diagnosis
Fluorescence probes
Human disease
Nucleic acid detection
Sensing platforms
Sensitive detection
Article
base pairing
colorimetry
DNA hybridization
high resolution transmission electron microscopy
human
Human immunodeficiency virus
human tissue
limit of detection
native polyacrylamide gel electrophoresis
nucleic acid analysis
particle size
prognosis
reproducibility
spectrofluorometry
zeta potential
genetic procedures
genetics
Biosensors
DNA (Cytosine-5-)-Methyltransferase
DNA, B-Form
DNA, A-Form
PY - 2021
ST - A novel disease-associated nucleic acid sensing platform based on split DNA-
scaffolded sliver nanocluster
T2 - Analytica Chimica Acta
TI - A novel disease-associated nucleic acid sensing platform based on split DNA-
scaffolded sliver nanocluster
85107979349&doi=10.1016%2fj.aca.2021.338734&partnerID=40&md5=92b2eabf2284fe2e543f65
aabd5579c2
VL - 1175
ID - 8700
ER -
TY - JOUR
AB - OBJECTIVE: Inflammatory bowel disease (IBD) is characterized by chronic
intestinal inflammation as a result of an exaggerated T-cell response. Cytotoxic T
lymphocyte antigen-4 (CTLA-4) expressed mainly on activated T cells, inhibits T
cell activation by combining B(7) through competing CD(28) and maintains immune
system homeostasis. Polymorphisms in the CTLA-4 gene are known to be associated
with several autoimmune diseases, but no studies related to IBD. The aim of the
study is to investigate an association between CTLA-4 gene microsatellite
polymorphisms and IBD. METHODS: Unrelated 68 Chinese Han patients with IBD (54
ulcerative colitis and 14 Crohn's disease) and 140 healthy controls were studied.
The (AT)n repeat sequence in the 3' untranslated region of exon 4 were amplified by
allele-specific PCR. The amplified products were electrophorosised by 12%
polyacrylamid gel and followed by silver staining. RESULTS: Eighteen alleles of
CTLA-4 microsatellite were found in Chinese patients and healthy individuals. Long
allele, 122bp was apparently increased in patients with ulcerative colitis compared
with healthy controls (7.4% vs 0.3%, P = 0.0002/Pc = Sig, OR = 22.32, 95% CI: 2.76
- 180.80). CONCLUSION: CTLA-4 gene microsatellite polymorphism was strongly
associated with ulcerative colitis in Chinese Han patients in Hubei province.
AN - rayyan-553782387
AU - Jiang, Y.
AU - Xia, B.
IS - 3
J2 - Zhonghua Nei Ke Za Zhi
KW - Adult
Antigens, CD
Antigens, Differentiation/*genetics
CTLA-4 Antigen
Female
Humans
Inflammatory Bowel Diseases/*genetics
Male
*Microsatellite Repeats
*Polymorphism, Genetic
T-Lymphocytes, Cytotoxic
LA - chi
N1 - Department of Internal Medicine, Wuhan University Zhongnan Hospital, Wuhan
430071, China.
PY - 2004
SN - 0578-1426 (Print)
SP - 191-4
ST - [Association between the cytotoxic T lymphocyte antigen-4 gene microsatellite
polymorphism and inflammatory bowel diseases in the Chinese]
T2 - Zhonghua nei ke za zhi
TI - [Association between the cytotoxic T lymphocyte antigen-4 gene microsatellite
polymorphism and inflammatory bowel diseases in the Chinese]
VL - 43
Y2 - 3
ID - 10294
ER -
TY - COMP
AB - OBJECTIVE: To investigate the effects of Fuzheng Huayu Recipe on renal
interstitial fibrosis in rats induced by the toxic substances. METHODS: Forty
Wistar rats were divided into 3 groups, including the normal group, model control
and Fuzheng Huayu Recipe treated group. The renal fibrotic model was induced with
intraperitoneal injection of dimethylnitrosamine (DMN) for 4 weeks and oral
administration of food containing heavy metals such as Hg, etc for 8 weeks. From
the 5th week, the rats were orally administered Fuzheng Huayu Recipe for 4 weeks.
The renal tissue was stained with HE and periodic acid-silver metheramine,
respectively. The serum creatinine and urea nitrogen were assayed with the kits,
and the renal hydroxyproline (Hyp) contents in the homogenate of kidney tissue were
measured with HCl hydrolysis. RESULTS: In the model group, the renal tubule was
swollen, and the tubular epithelial cells were degenerated, necrosed and detached,
with the infiltration of inflammatory cells; the tubular interstitium was expanded,
characterized with the deposition of large collagens; the tubular basement membrane
became thicker and wrinkled; and the renal Hyp content and serum creatinine and
urea nitrogen levels increased remarkably as compared to the normal group. After
the treatment with Fuzheng Huayu Recipe, the renal tubular inflammation and
interstitial collagen deposition were alleviated, and the serum creatinine and urea
nitrogen levels decreased significantly as compared to the model group. CONCLUSION:
Administration of DMN and heavy metals can induce the renal interstitial fibrosis
and impair the renal function. Fuzheng Huayu Recipe can improve the impaired renal
functions and reverse the renal interstitial fibrosis.
AN - rayyan-553782302
AU - Jiang, Z. H.
AU - Cui, H. Y.
AU - Liu, C. H.
AU - Liu, C.
AU - Liu, P.
AU - Xu, L. M.
AU - Hu, Y. Y.
AU - Yuan, J. L.
AU - Li, F. H.
CY - China
DO - 10.3736/jcim20040514
ET - 5
J2 - Zhong Xi Yi Jie He Xue Bao
KW - Animals
Creatinine/blood
Dimethylnitrosamine/toxicity
Drugs, Chinese Herbal/*therapeutic use
Fibrosis
Hydroxyproline/analysis
Kidney/*drug effects/pathology/physiopathology
Male
Nephritis, Interstitial/chemically induced/drug therapy
Rats
Rats, Wistar
Renal Insufficiency/chemically induced/drug therapy
LA - chi
N1 - Institute of Liver Diseases, Shanghai University of Traditional Chinese
Medicine, Shanghai 201203, China. chenghai_liu@yahoo.com.cn
PY - 2004
SN - 1672-1977 (Print)
SP - 358-60
ST - [Effects of Fuzheng Huayu recipe on renal toxic interstitial fibrosis in
rats]
T2 - Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
TI - [Effects of Fuzheng Huayu recipe on renal toxic interstitial fibrosis in
rats]
VL - 2
Y2 - 9
ID - 10210
ER -
TY - JOUR
AB - Objective： To investigate the intervention effect of Danggui Buxuetang on
oxidative stress and inflammatory response in diabetic kidney disease （DKD） rats
from its improvement of podocyte mitochondrial dysfunction. Method：SD rats were
randomly divided into the control group and modeling group， and the ones in the
latter group rats were fed a high-glucose and high-fat diet and then
intraperitoneally injected with a small dose of streptozotocin（STZ）for inducing
type 2 diabetes. The successfully modeled rats were randomized into the model
group，high-and low-dose（1.44 and 0.72 g·kg-1）Danggui Buxuetang groups，and
irbesartan（0.017 g·kg-1）group and gavaged with the corresponding drugs，while
those in the normal and model groups with an equal volume of normal saline. After
20 weeks of drug intervention，the urinary microalbumin-tourine creatinine ratio
（UACR） and serum malondialdehyde （MDA） content and manganese superoxide
dismutase（MnSOD）activity in each group were measured. The pathological changes in
renal tissue were observed by Masson trichrome staining，and periodic acid-silver
metheramine（PASM）staining，followed by the observation of ultrastructural changes
in podocytes under the transmission electron microscope（TEM）. The expression
level of reactive oxygen species（ROS）in rat kidney tissue was detected using a
fluorescent probe dihydroethidium （DHE）. The protein expression levels of
peroxisome proliferator-activated receptor γ coactivator-1α（PGC-1α），nucleotide-
binding domain like receptor protein 3（NLRP3），and Wilms tumor protein-1（WT-
1）were measured by immunohistochemistry（IHC），and the expression levels of
NLRP3， interleukin-1β（IL-1β），and WT-1 in podocytes by
immunofluorescence（IF）assay. The mRNA expression levels of PGC-1α and NLRP3 in
the renal tissues were determined by real-time fluorescence quantitative polymerase
chain reaction（Real-time PCR），and the protein expression levels of PGC-
1α，MnSOD，NLRP3，and IL-1β were assayed by Western blot. Result： Compared with the
normal group，the model group exhibited elevated UACR and MDA content，weakened
MnSOD activity（P<0.01），glomerular hypertrophy，thickened basement membrane，
mesangial hyperplasia， increased extracellular matrix， K-W nodules， podocyte
mitochondrial swelling， disordered mitochondrial cristae， foot process fusion or
loss， vacuolization， increased ROS （P<0.01），enhanced NLRP3 and IL-1β but
diminished WT-1 expression in podocytes，down-regulated PGC-1α mRNA
expression（P<0.01）and PGC-1α and MnSOD protein expression（P<0.01），and up-
regulated NLRP3 mRNA expression and NLRP3 and IL-1β protein expression（P<0.01）.
Compared with the model group， Danggui Buxuetang high-dose group significantly
decreased UACR and MDA，enhanced MnSOD activity （P<0.05，P<0.01），improved renal
histopathology and podocyte mitochondrial ultrastructure，decreased ROS
（P<0.05，P<0.01）and NLRP3 and IL-1β expression in podocytes，enhanced WT-1
expression in podocytes，upregulated the mRNA and protein levels of PGC-1α and
MnSOD，and down-regulated the mRNA and protein levels of NLRP3 and IL-
1β（P<0.05，P<0.01）. Conclusion： Danggui Buxuetang alleviates oxidative stress，
·32· reduces inflammatory response，protects kidney，and delays the progression of
DKD possibly by improving the mitochondrial dysfunction in podocytes of DKD rats. ©
2022, China Academy of Chinese Medical Sciences Institute of Chinese Materia
Medica. All rights reserved.
AN - rayyan-553780681
AU - Jin, H. C.
AU - Qiang, J. W.
AU - Zhang, G. W.
AU - Liang, S. R.
AU - Guo, D. Z.
DO - 10.13422/j.cnki.syfjx.20212442
IS - 3
KW - Danggui Buxuetang
diabetic kidney disease （DKD）
inflammatory response
mitochondrial dysfunction
podocytes；oxidative stress
adegramotide
Chinese drug
cryopyrin
Danggui Buxue Tang
interleukin 1beta
irbesartan
malonaldehyde
manganese superoxide dismutase
peroxisome proliferator activated receptor gamma coactivator 1alpha
streptozocin
unclassified drug
albumin to creatinine ratio
animal experiment
animal model
animal tissue
apoptosis
Article
controlled study
drug megadose
enzyme activity
fluorescence quantitative polymerase chain reaction
gene expression
histopathology
inflammation
lipid diet
low drug dose
nonhuman
oxidative stress
podocyte
protein expression
rat
Western blotting
Kidney Diseases
Kidney
Oxidative Stress
Diabetic Nephropathies
Rats
Inflammation
PY - 2022
SP - 31-40
ST - Danggui Buxuetang Alleviates Oxidative Stress and Inflammation in Diabetic
Kidney Disease Rats by Improving Mitochondrial Dysfunction of Podocytes
T2 - Chinese Journal of Experimental Traditional Medical Formulae
TI - Danggui Buxuetang Alleviates Oxidative Stress and Inflammation in Diabetic
Kidney Disease Rats by Improving Mitochondrial Dysfunction of Podocytes
85129696156&doi=10.13422%2fj.cnki.syfjx.20212442&partnerID=40&md5=ea68017d59bbbca24
d269d3d87dc7509
VL - 28
ID - 8704
ER -
TY - JOUR
AB - Mast cells play critical roles in allergic disorders such as atopic
dermatitis and allergic asthma. The aim of this study was to investigate the anti-
inflammatory and anti-asthmatic activities of 1,6-O,O-diacetylbritannilactone
(OODBL) isolated from Inula japonica Thunb. (I. japonica) in a murine asthma model
and bone marrow-derived mast cells (BMMCs). In an ovalbumin-induced asthma model,
OODBL administration attenuated the airway hyper-responsiveness induced by
aerosolized methacholine and serum IgE level in asthmatic mice. In vitro system, we
found that OODBL reduced leukotriene C4 production and degranulation through the
suppression of cytosolic phospholipase A2 phosphorylation and phospholipase Cγ-
mediated Ca2+ influx in IgE/antigen-stimulated BMMCs. Taken together, OODBL may
have therapeutic potential in the treatment of allergic diseases such as asthma. ©
2017 Informa UK Limited, trading as Taylor & Francis Group.
AN - rayyan-553780682
AU - Jin, M.
AU - Kim, S.
AU - Qin, N.
AU - Chen, X.
AU - Ji, N.
AU - Tang, S. A.
AU - Kong, D.
AU - Lee, E.
AU - Duan, H.
DO - 10.1080/08923973.2017.1318911
IS - 4
KW - asthma
inflammation
leukotriene C4
OODBL
Animals
Anti-Allergic Agents
Anti-Asthmatic Agents
Asthma
Bone Marrow Cells
Cell Degranulation
Dermatitis, Atopic
Female
Immunoglobulin E
Lactones
Leukotriene C4
Mast Cells
Mice
Mice, Inbred BALB C
Ovalbumin
Phosphorylation
Respiratory Hypersensitivity
Sesquiterpenes
Signal Transduction
1,6 o,o diacetylbritannilactone
antiasthmatic agent
beta n acetylhexosaminidase
calcium
dexamethasone
immunoglobulin E
membrane phospholipid
methacholine
montelukast
ovalbumin
phospholipase A2
phospholipase C gamma
silver
unclassified drug
antiallergic agent
lactone
O, O-diacetylbritannilactone
sesquiterpene
animal cell
animal experiment
animal model
anti asthmatic activity
Article
basophil degranulation
bone marrow derived mast cell
calcium cell level
calcium transport
cell activation
cell viability
controlled study
cytotoxicity
drug activity
enzyme phosphorylation
female
IC50
immunoglobulin blood level
in vitro study
Inula
Inula japonica
male
mast cell
mouse
MTS assay
nonhuman
priority journal
animal
atopic dermatitis
Bagg albino mouse
bone marrow cell
chemically induced
degranulation
disease model
drug effects
metabolism
phosphorylation
respiratory tract allergy
signal transduction
PY - 2017
SP - 173-179
ST - 1,6-O,O-Diacetylbritannilactone suppresses activation of mast cell and airway
hyper-responsiveness
T2 - Immunopharmacology and Immunotoxicology
TI - 1,6-O,O-Diacetylbritannilactone suppresses activation of mast cell and airway
hyper-responsiveness
85018186234&doi=10.1080%2f08923973.2017.1318911&partnerID=40&md5=9dd31abfd570be669b
8c57a4f170a97b
VL - 39
ID - 8705
ER -
TY - JOUR
AB - This review is concerned with evaluating the toxicity associated with human
exposure to silver and gold nanoparticles (NPs), due to the relative abundance of
toxicity data available for these particles, when compared to other metal
particulates. This has allowed knowledge on the current understanding of the field
to be gained, and has demonstrated where gaps in knowledge are. It is anticipated
that evaluating the hazards associated with silver and gold particles will
ultimately enable risk assessments to be completed, by combining this information
with knowledge on the level of human exposure. The quantity of available hazard
information for metals is greatest for silver particulates, due to its widespread
inclusion within a number of diverse products (including clothes and wound
dressings), which primarily arises from its antibacterial behaviour. Gold has been
used on numerous occasions to assess the biodistribution and cellular uptake of NPs
following exposure. Inflammatory, oxidative, genotoxic, and cytotoxic consequences
are associated with silver particulate exposure, and are inherently linked. The
primary site of gold and silver particulate accumulation has been consistently
demonstrated to be the liver, and it is therefore relevant that a number of in
vitro investigations have focused on this potential target organ. However, in
general there is a lack of in vivo and in vitro toxicity information that allows
correlations between the findings to be made. Instead a focus on the tissue
distribution of particles following exposure is evident within the available
literature, which can be useful in directing appropriate in vitro experimentation
by revealing potential target sites of toxicity. The experimental design has the
potential to impact on the toxicological observations, and in particular the use of
excessively high particle concentrations has been observed. As witnessed for other
particle types, gold and silver particle sizes are influential in dictating the
observed toxicity, with smaller particles exhibiting a greater response than their
larger counterparts, and this is likely to be driven by differences in particle
surface area, when administered at an equal-mass dose. A major obstacle, at
present, is deciphering whether the responses related to silver nanoparticulate
exposure derive from their small size, or particle dissolution contributes to the
observed toxicity. Alternatively, a combination of both may be responsible, as the
release of ions would be expected to be greater for smaller particles. © 2010
Informa UK Ltd.
AN - rayyan-553780684
AU - Hutchison, G.
AU - Christensen, F. M.
AU - Peters, S.
AU - Hankin, S.
AU - Stone, V.
DO - 10.3109/10408440903453074
IS - 4
KW - Gold
Metals
Nanoparticle
Nanotoxicology
Silver
Dust
Humans
Nanoparticles
Particle Size
Particulate Matter
Risk Assessment
Silver Compounds
Tissue Distribution
amoxicillin
gold nanoparticle
metronidazole
nanoparticle
silver nanoparticle
sulfadiazine silver
argyria
arthritis
burn
clinical trial
controlled study
cytotoxicity
diabetes mellitus
drug absorption
drug distribution
drug effect
drug transport
genotoxicity
human
inflammation
intestine ulcer
liver toxicity
lung toxicity
nanotoxicology
neoplasm
nonhuman
oxidative stress
particle size
review
risk assessment
skin discoloration
tissue distribution
toxicity
toxicity testing
wound dressing
PY - 2010
SP - 328-346
ST - A review of the in vivo and in vitro toxicity of silver and gold
particulates: Particle attributes and biological mechanisms responsible for the
observed toxicity
T2 - Critical Reviews in Toxicology
TI - A review of the in vivo and in vitro toxicity of silver and gold
particulates: Particle attributes and biological mechanisms responsible for the
observed toxicity
77949780454&doi=10.3109%2f10408440903453074&partnerID=40&md5=93f061be04dc41114d6316
5eabf8a5ed
VL - 40
ID - 8707
ER -
TY - JOUR
AB - Ultra-fine grained biodegradable Mg-based Mg1Zn1Mn0.3 Zr - HA and
Mg4Y5.5Dy0.5 Zr - 45S5 Bioglass composites have shown great medical potential. Two
types of these Mg-based biomaterials subjected to different treatments were tested
and as shown earlier they are biocompatible. The aim of the study is to determine
how much culture media incubated with these ultra-fine trained Mg-based composites
can cause inflammatory reactions and /or periodontal cell death. The incubation of
composites in the medium releases metal ions into the solution. It can be assumed
that this process is permanent and also occurs in the human body. The results have
shown that the effect of proinflammatory IL-6 and TNF-[Formula presented] cytokines
results in the strongest production of the acute phase proteins in the first day on
the Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. % Ag HF-treated biocomposite after immersion for
2 h in 40 % HF and then the fastest decrease in these processes on the third day.
In turn, the inflammatory process induced on the Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. %
Ag biomaterial, in BAX / BCL ratio assessment, is the strongest on the third day
and maintains a significantly high level on the following day, which, at the same
time, confirms its persistence and development. In addition, these results confirm
the successively generated necrotic processes. Ions can induce inflammatory
reactions, which in the case of the implant may take a long time, which results in
the loss of the implant. Even if the material is biocompatible in rapid in-vitro
tests, it can induce inflammation in the body after some time due to the release of
ions. Not every treatment improves the material's properties in terms of subsequent
safety. [Formula presented] © 2019
AN - rayyan-553780686
AU - Jurczyk, M. U.
AU - Żurawski, J.
AU - Wirstlein, P. K.
AU - Kowalski, K.
AU - Jurczyk, M.
DO - 10.1016/j.micron.2019.102796
KW - Biomaterials
Cytokines: IL-6 and TNF-α
Inflammatory cells
Mg
Cells, Cultured
Ceramics
Glass
Humans
Inflammation
Interleukin-6
Magnesium
Magnesium Compounds
Materials Testing
Osteoblasts
Periodontium
Surface Properties
Biocompatibility
Cell death
Dysprosium alloys
Manganese alloys
Metal ions
Proteins
Silver
Zinc alloys
Zirconium
bioactive glass 45S5
biomaterial
glass
IL6 protein, human
interleukin 6
magnesium
magnesium derivative
Acute phase proteins
Cytokines
Different treatments
Inflammatory process
Mg based composites
Ultra-fine-grained
biosynthesis
cell culture
ceramics
cytology
drug effect
human
inflammation
materials testing
osteoblast
periodontium
pharmacology
procedures
surface property
Magnesium alloys
PY - 2020
ST - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
T2 - Micron
TI - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
85076014504&doi=10.1016%2fj.micron.2019.102796&partnerID=40&md5=cc127a234db0df5ceaa
609f319702eda
VL - 129
ID - 8709
ER -
TY - JOUR
AB - Ultra-fine grained biodegradable Mg-based Mg1Zn1Mn0.3 Zr - HA and
Mg4Y5.5Dy0.5 Zr - 45S5 Bioglass composites have shown great medical potential. Two
types of these Mg-based biomaterials subjected to different treatments were tested
and as shown earlier they are biocompatible. The aim of the study is to determine
how much culture media incubated with these ultra-fine trained Mgbased composites
can cause inflammatory reactions and /or periodontal cell death. The incubation of
composites in the medium releases metal ions into the solution. It can be assumed
that this process is permanent and also occurs in the human body. The results have
shown that the effect of proinflammatory IL-6 and TNF-e cytokines results in the
strongest production of the acute phase proteins in the first day on the
Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. % Ag HF-treated biocomposite after immersion for 2 h
in 40 % HF and then the fastest decrease in these processes on the third day. In
turn, the inflammatory process induced on the Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. % Ag
biomaterial, in BAX / BCL ratio assessment, is the strongest on the third day and
maintains a significantly high level on the following day, which, at the same time,
confirms its persistence and development. In addition, these results confirm the
successively generated necrotic processes. Ions can induce inflammatory reactions,
which in the case of the implant may take a long time, which results in the loss of
the implant. Even if the material is biocompatible in rapid in-vitro tests, it can
induce inflammation in the body after some time due to the release of ions. Not
every treatment improves the material's properties in terms of subsequent safety.
AN - rayyan-553780687
AU - Jurczyk, M. U.
AU - Zurawski, J.
AU - Wirstlein, P. K.
AU - Kowalski, K.
AU - Jurczyk, M.
DO - 10.1016/j.micron.2019.102796
PY - 2020
SN - 0968-4328 1878-4291
ST - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
T2 - MICRON
TI - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
VL - 129
Y2 - 2
ID - 8710
ER -
TY - JOUR
AB - Factors involved in wound healing and their interdependence are not yet fully
understood; nevertheless, new prospects for therapy to favor speedy and optimal
healing are emerging. Reports about wound healing modulation by local application
of simple and natural agents abound even in the recent literature, however, most
are anecdotal and lack solid scientific evidence. We describe the effect of silver
sulfadiazine and moist exposed burn ointment (MEBO), a recently described burn
ointment of herbal origin, on mast cells and several wound healing cytokines (bFGF,
IL-1, TGF-beta, and NGF) in the rabbit experimental burn model. The results
demonstrate that various inflammatory cells, growth factors and cytokines present
in the wound bed may be modulated by application of local agents with drastic
effects on their expression dynamics with characteristic temporal and spatial
regulation and changes in the expression pattern. Such data are likely to be
important for the development of novel strategies for wound healing since they shed
some light on the potential formulations of temporally and combinatory optimized
therapeutic regimens. (C) 2006 Elsevier Ltd and ISBI. All rights reserved.
AN - rayyan-553780688
AU - Jurjus, A.
AU - Atiyeh, B. S.
AU - Abdallah, I. M.
AU - Jurjus, R. A.
AU - Hayek, S. N.
AU - Abou Jaoude, M.
AU - Gerges, A.
AU - Tohme, R. A.
DO - 10.1016/j.burns.2006.10.406
IS - 7
KW - Wound Healing
Burns
PY - 2007
SN - 0305-4179 1879-1409
SP - 892-907
ST - Pharmacological modulation of wound healing in experimental burns
T2 - BURNS
TI - Pharmacological modulation of wound healing in experimental burns
VL - 33
Y2 - 11
ID - 8711
ER -
TY - JOUR
AB - The objective of this review is to form a short compilation of phytochemical
screening, pharmaceutical and pharmacological profile of the plant Cochlospermum
religiosum. Although the plant is of importance and is widely used in traditional
system of medicine, a review article based on the phytochemical and pharmacological
screening of Cochlospermum religiosum is not upto date not reported. The various
histochemical studies revealed that this plant contains numerous primary and
secondary metabolites as its constituents such as flavanoids, steroids, tannins,
glycosides, alkaloids, phenols, starch grains and crystals. The gum Katira obtained
from the stem bark of Cochlospermum religiosum has wide variety of applications in
pharmaceutical industry as adjuvant in colon targeted drug delivery formulations
like Azathioprine, as a matrix coating polymers, suspending agent and sustain
release adjuvant for nimusulide and Etodalac formulations respectively. The silver
nanoparticles biosynthesized from this plant are found to possess antimicrobial
activity against various pathogenic microbes. Moreover, many research studies have
been conducted to prove the plant's potential as antimicrobial agents. The
bioactive secondary metabolite Myricetin which has wide array of biochemical
properties, such as antineoplastic, anti-carcinogenic, antioxidant activity and
anti-inflammatory effects has been identified and isolated from leaf and callus by
using different techniques such as IR spectra and HPTLC. This review focuses on the
phytochemistry, pharmaceutical and pharmacological actions of Cochlospermum
religiosum.
AN - rayyan-553780689
AU - Jyothi, Y.
AU - Sangeetha, D.
IS - 5
KW - Cochlospermum religiosum
Gum Katira
Myrcetin
Yello silk tree
alkaloid
Cochlospermum religiosum extract
flavanoid
glycoside
phenol
plant extract
silver nanoparticle
steroid
tannin
unclassified drug
Alstonia
Bacillus subtilis
bark
callus (plant)
cell viability
drug cytotoxicity
drug isolation
drug mechanism
drug screening
Enterobacter aerogenes
Escherichia coli
histochemistry
in vitro study
liver protection
Micrococcus
nonhuman
phytochemistry
plant leaf
plant stem
Proteus vulgaris
Review
Xanthomonas axonopodis
Xanthomonas oryzae
Bixaceae
PY - 2016
SP - 272-277
ST - Cochlospermum religiosum (Linn): A phytopharmacological review
T2 - Research Journal of Pharmaceutical, Biological and Chemical Sciences
TI - Cochlospermum religiosum (Linn): A phytopharmacological review
84987750969&partnerID=40&md5=25e3df5b805d54308b527d4b611fa7d5
VL - 7
ID - 8712
ER -
TY - JOUR
AB - Objective. To study the current Russian and foreign literature dedicated to
the problem of application of organometallic compounds immobilized on drug delivery
in the treatment of purulent-inflammatory disease of the skin and soft tissues.
Methods. The modern Russian and foreign literature, available in the Pubmed,
Medline, Springer, Scopus, e-LIBRARY databases were reviewed according to the
problems of purulent-inflammatory diseases, skin and soft tissue infections, the
integrated approach to the treatment of purulent-inflammatory diseases, synthesis,
immobilized organometallic compounds. Results. The observational study of the
specific recent achievements in the modification of antimicrobial biomaterials is
presented. Metal ions have a broad range of antimicrobial activity (especially on
proliferation and remodeling), possess by bacteriostatic and bactericidal effect,
demonstrate multiple inhibitory effects against bacterial strains and have been
proven effective in improving wound healing in all its phases. Natural products and
especially biologically active metals such as silver, copper, zinc and germanium,
are believed to be an alternative for the development of perspective biomaterials
with antimicrobial properties. In recent years, new approach for the production and
application of therapeutic and diagnostic drugs based on the immobilization or
grafting of drug substances on polymer carriers has been developed. At present,
namely the immobilized compounds that have opened the way to the creation of
prolonged-action drugs with low toxicity and allergenicity. Conclusion. Template
synthesis of new organometallic drug compounds is considered to be a promising
direction in the wound infection treatment, which requires further experimental and
clinical study. © 2021 Vitebsk State Medical University. All rights reserved.
AN - rayyan-553780690
AU - Kadomtsevа, A. V.
AU - Zarubenko, P. A.
AU - Loginova, L. B.
DO - 10.18484/2305-0047.2021.3.334
IS - 3
KW - Biometals
Immobilized compounds
Nanoparticles
Organometallic frameworks
Purulent-inflammatory processes
Synthesis
Skin Diseases
PY - 2021
SP - 334-346
ST - The role of immobilized metal-organic compounds in the complex treatment of
purulent-inflammatory disease of skin and soft tissues
T2 - Novosti Khirurgii
TI - The role of immobilized metal-organic compounds in the complex treatment of
purulent-inflammatory disease of skin and soft tissues
85111759564&doi=10.18484%2f2305-
0047.2021.3.334&partnerID=40&md5=5fcfa443a09b6ff8471e9f5d4ab619fe
VL - 29
ID - 8713
ER -
TY - JOUR
AB - To study acute lung toxicity of various doses of colloidal silver
nanoparticles (Ag-NPs), mice were intratracheally instilled with 0, 10, 100, 1000
or 10,000 ppm of Ag-NPs. Histopathology, autometallography (AMG) and
immunohistochemistry were determined at 1, 3, 7 and 15 days post-
exposure.Instillation of 100, 1,000 and 10,000 ppm Ag-NPs produced moderate to
severe necrotizing bronchitis and alveolitiswith hypertrophy and hyperplasia of
alveolar epithelial cells. The severity of the pulmonary inflammation and damage
increased in a dose-dependent manner. Concomitant lamininimmunohistochemical
findings generally correlated with pulmonary lesions. Interleukin 1- beta (IL-1β)
and tumor necrotic factor-alpha (TNF-α) positive immunostaining were found in the
inflammatory lesions in lungs of treated animals. Superoxide dismutase (SOD) and
metallothionine (MT) expression occurred in particle laden AMs and lung epithelial
cells, which correlated with inflammatory sites and particle aggregated areas. AMG
gains were found inparticle laden AMs, alveolar epithelial cells and macrophages in
hilarlymph nodes. These findings suggest that instillation of AgNPs causes acute
lung inflammation and tissue damage in a concentration-dependent manner. IL-1β and
TNF-α may involve in the pathogenesis of the acute lung toxicity. Oxidative stress
may underlie the lung tissue injury. Moreover, the expression of MT in tissues
responded to AgNPs accumulation.
AN - rayyan-553780691
AU - Kaewamatawong, T.
AU - Banlunara, W.
AU - Maneewattanapinyo, P.
AU - Thammacharoen, C.
AU - Ekgasit, S.
IS - 3
KW - Acute
Colloidal silver nanoparticles
Lung toxicity
Mouse
Mice
PY - 2013
SP - 383-390
ST - Acute pulmonary toxicity caused by single intratracheal instillation of
various doses of colloidal silver nanoparticles in mice: Pathological changes,
particle bioaccumulation and metallothionien protein expression
T2 - Thai Journal of Veterinary Medicine
TI - Acute pulmonary toxicity caused by single intratracheal instillation of
various doses of colloidal silver nanoparticles in mice: Pathological changes,
particle bioaccumulation and metallothionien protein expression
84897930059&partnerID=40&md5=d9e3b100c4c3f2ee6323e4bcae74fc3b
VL - 43
ID - 8714
ER -
TY - JOUR
AB - Morphological changes of liver and spleen under the impact of dextran-
polyacrylamide polymers and their effects as carriers of silver and gold
nanoparticles. Kaleinikova O.M., Kurovska V.O., Byelinska I.V., Kutsevol N.V.,
Blashkiv T. V. The possibility of usage of polymer nanocomposites is being
intensively studied today with a purpose of their application in medicine,
espessialy in oncology. At the experimental stage it is important to determine the
mechanisms of the influence of such compounds on the bod V and their own possible
undesirable effects. Aim - to study the effect of the treatment with maximal doses
of the dextran-polyacrylamide polymers and their effect as carriers of silver and
gold nanoparticles on the spleen and liver. Histological examination of
micropreparations of the spleen and liver by the standard method with hematoxylin-
eosin staining was made. As a result of the treatment with nonionic (D-g-PAA) and
anionic (D-g-PAA (PE» polymer matrices, changes which occurred in the spleen
indicate an increase in the production of all blood cells. These phenomena were
absent when silver and gold nanoparticles were included in the matrix. In the
liver, treatment with D-g-PAA and D-g-PAA (PE) caused a disorder of hepatic
circulation, focal infiltration by inflammatory cells and death of liepatocytes by
necrosis. The addition of nanoparticles triggered other mechanisms of alteration,
which manifested themselves in excessive accumulation of glycogen, fatty
infiltration ofhepatocytes, and cell death, mainly through apoptosis. However;
along with this, sigfis of an incomplete regenerative response of the liver were
revealed. Morphological changes caused by the treatment with maximal doses of the
tested substances indicate their toxic effect, especially on the liver. Further
researches are needed to establish the optimal doses and the frequency of their
administration, which can be used for therapeutic purposes, including the
interaction of studied polymers with blood cells. © 2023 Dnipro State Medical
University. All rights reserved.
AN - rayyan-553780692
AU - Kaleinikova, O. M.
AU - Kurovska, V. O.
AU - Byelinska, I. V.
AU - Kutsevol, N. V.
AU - Blashkiv, T. V.
DO - 10.26641/2307-0404.2023.1.275855
IS - 1
KW - dextran-polyacrylamide polymers
liver
silver and gold nanoparticles
spleen
toxicity
Liver
Polymerization
Polymers
Dextrans
Spleen
PY - 2023
SP - 28-36
ST - MORPHOLOGICAL CHANGES OF SPLEEN AND LIVER UNDER THE IMPACT OF DEXTRAN-
POLYACRYLAMIDE POLYMERS AND THEIR EFFECTS AS CARRIERS OF SILVER AND GOLD
NANOPARTICLES
T2 - Medicni Perspektivi
TI - MORPHOLOGICAL CHANGES OF SPLEEN AND LIVER UNDER THE IMPACT OF DEXTRAN-
POLYACRYLAMIDE POLYMERS AND THEIR EFFECTS AS CARRIERS OF SILVER AND GOLD
NANOPARTICLES
85160039576&doi=10.26641%2f2307-
0404.2023.1.275855&partnerID=40&md5=fb2b442345c61836239a2ab7be222b3b
VL - 28
ID - 8715
ER -
TY - COMP
AB - In addition to toxic reactions to dental materials, some individuals may
develop or exhibit hypersensitivity reactions to leachable components. An
experimental model combining the guinea pig maximization test for induction of
hypersensitivity and the subcutaneous implantation of dental cements is described.
Guinea pigs immunized with AH 26, an epoxy-bisphenol resin, showed an increased
tissue response to AH26 implants. Guinea pigs immunized with zinc oxide-eugenol did
not show a similarly increased response, possibly because of an anti-inflammatory
effect of eugenol. The experimental model may prove useful in predicting the effect
of leachable allergens from dental materials in sensitized individuals.
AN - rayyan-553782230
AU - Kallus, T.
AU - Hensten-Pettersen, A.
AU - Mjör, I. A.
CY - United States
DO - 10.1002/jbm.820170503
ET - 5
J2 - J Biomed Mater Res
KW - *Allergens
Animals
Bismuth/adverse effects
Dental Materials/*adverse effects/immunology
Drug Combinations/adverse effects
*Epoxy Resins
Evaluation Studies as Topic
Female
Guinea Pigs
Hypersensitivity/*etiology/pathology
Immunization
Methenamine/adverse effects
Root Canal Filling Materials
Silver/adverse effects
Skin Tests
Titanium/adverse effects
Zinc Oxide-Eugenol Cement/adverse effects
Allergens
LA - eng
PY - 1983
SN - 0021-9304 (Print)
SP - 741-55
ST - Tissue response to allergenic leachables from dental materials
T2 - Journal of biomedical materials research
TI - Tissue response to allergenic leachables from dental materials
VL - 17
Y2 - 9
ID - 10140
ER -
TY - JOUR
AB - Cancer is an abnormal growth of cells due to the uncontrolled division of the
cells and it is the second leading cause of death globally. Immune system
malfunction, inflammatory diseases, microbial infection, and oxidative damage are
other causes for death. This prompted us to search for non-traditional materials
that can be used as anti-cancer, anti-microbial, anti-oxidant and anti-inflammatory
agents. Chitosan as a non-toxic, biodegradable, biopolymer with powerful biological
activity was grafted with acidified 2-aminothiophenol (2-ATH) using aqueous
chemical oxidative copolymerization in presence of ammonium persulphate (APS) as an
oxidant at room temperature. The prepared polymeric samples were assembled on
silver nanoparticles (AgNPs). The prepared polymeric structure nano-composites were
verified by infrared, ultraviolet-visible spectroscopy, X-ray diffraction,
thermogravimetric analysis scanning electron microscopy and transmission electron
microscopy. The prepared polymeric samples and their composites with AgNPs were
screened for their biological activities as anti-cancer properties, anti-microbial,
anti-oxidant and anti-inflammatory. The obtained data reveal that chitosan-gr-poly
(2-aminothiophenol) (chitosan-gr-p2-ATH) has the most potent anti-oxidant and anti-
cancer effects against HepG2 while the composites of p2-ATH with AgNPs and
chitosan-gr-p2-ATH with AgNPs have the most potent anti-inflammatory properties.
AN - rayyan-553782139
AU - Kamel, E. M.
AU - Ahmed, O. M.
AU - Abd El-Salam, H. M.
DO - 10.1016/j.ijbiomac.2020.09.140
J2 - Int J Biol Macromol
KW - Ammonium Sulfate/chemistry/pharmacology
Chitosan/*chemistry/therapeutic use
Humans
Immune System/drug effects
Metal Nanoparticles/*chemistry/therapeutic use
Neoplasms/drug therapy
Oxidation-Reduction/drug effects
Polymers/*chemistry/therapeutic use
Silver/chemistry
Polymerization
Polymers
LA - eng
N1 - Department of Chemistry, Faculty of Science, Polymer Research Laboratory,
Beni-Suef University, 62514 Beni-Suef City, Egypt.; Physiology Division, Zoology
Department, Faculty of Science, Beni-Suef University, Beni-Suef, P.O.Box 62521,
Egypt.; Department of Chemistry, Faculty of Science, Polymer Research Laboratory,
Beni-Suef University, 62514 Beni-Suef City, Egypt. Electronic address:
orhanafy011246@science.bsu.edu.eg.
PY - 2020
SP - 2649-2659
ST - Fabrication of facile polymeric nanocomposites based on chitosan-gr-P2-
aminothiophenol for biomedical applications
T2 - International journal of biological macromolecules
TI - Fabrication of facile polymeric nanocomposites based on chitosan-gr-P2-
aminothiophenol for biomedical applications
VL - 165
Y2 - 12 y3 - 15
ID - 10050
ER -
TY - JOUR
AB - The analgesic, anti-pyretic and anti-inflammatory effects of FI-302, Ar-(3-
pip-eridinopropyl)-4-methyl-6-trifluoromethyl-furo[3,2-ft]indole-2-carboxamide, a
newly synthesized tricyclic compound, were investigated in comparison with those of
nonsteroidal anti-inflammatory drugs (NSAIDs). FI-302 showed potent analgesic and
antipyretic effects in the various models used. FI-302 showed an inhibitory effect
on acute inflammatory response such as carrageenin-induced edema, but did n ot show
any effect on chronic inflammatory response such as cotton pellet granuloma. These
effects of FI-302 were about 2 to 7 times more potent than those of non-a cidic
NSAIDs such as mepirizole and tiaramide. Moreover, FI-302 had little or no
ulcerogenic activity. From these results, it is conceivable that the spectrum of FI
-302's activities is similar to those of non-acidic NSAIDs such as mepirizole ana
tiaramide. These results suggest that FI-302 is a new non-ulcerogenic anti-inflamm
atory compound, and should be suitably applicable for clinical purposes. © 1985,
The Pharmaceutical Society of Japan. All rights reserved.
AN - rayyan-553780693
AU - Kameyama, T.
AU - Amanuma, F.
AU - Okuyama, S.
AU - Higuchi, S.
DO - 10.1248/bpb1978.8.477
IS - 6
KW - analgesic effect
anti-inflammatory effect
antipyretic effect
iV-(3-piperidinopropyl)-4-methyl-6-trifluoromethyl-furo[3,2- b]-indole-2-
carboxamide
ulcerogenic activity
Animals
Capillary Permeability
Edema
Erythema
Granuloma
Guinea Pigs
Indoles
Lethal Dose 50
Male
Mice
Rats
Stomach Ulcer
4 methyl 6 trifluoromethylfuro[3,2 b]indole 2 [n (3 piperidinopropyl)]carboxamide
acetic acid
acetylcholine
adjuvant
aminophenazone
epirizole
flufenamic acid
histamine
ibuprofen
indometacin
mefenamic acid
new drug
phenylbutazone
phenylquinone
silver nitrate
tiaramide
unclassified drug
analgesia
animal experiment
animal model
article
biological model
dose response
drug comparison
drug efficacy
drug mechanism
drug response
drug screening
drug toxicity
fever
inflammation
intoxication
intradermal drug administration
intraperitoneal drug administration
mouse
nervous system
nonhuman
rat
soft tissue
stomach
subcutaneous drug administration
ulcerogenesis
yeast
Animal Shells
PY - 1985
SP - 477-486
ST - Pharmacological studies of furo [3,2- b] indole derivatives. i. analgesic,
anti-pyretic and antiinflammatory effects of fi-302, n- (3-piperidino-propyd-4-
methyl-6-trifluoromethyl-furo [3,2-b] indole-2-carboxamide, in experimental animals
T2 - Journal of Pharmacobio-Dynamics
TI - Pharmacological studies of furo [3,2- b] indole derivatives. i. analgesic,
anti-pyretic and antiinflammatory effects of fi-302, n- (3-piperidino-propyd-4-
methyl-6-trifluoromethyl-furo [3,2-b] indole-2-carboxamide, in experimental animals
0021796022&doi=10.1248%2fbpb1978.8.477&partnerID=40&md5=0b30f4dc0008e33a4e8c76a3e60
91ed6
VL - 8
ID - 8716
ER -
TY - JOUR
AB - Chronic inflammatory conditions can negatively impact intestinal barrier
function and affect the epithelium's interaction with nano-sized materials. We
demonstrate the application of a Caco-2/THP-1 co-culture mimicking the intestine in
healthy (i.e. stable) or inflamed state in nanotoxicological research. The co-
cultures were exposed to non-toxic concentrations of silver nanoparticles (AgNPs)
or silver nitrate (AgNO3) for 24 h. The barrier integrity and cytokine release as
well as necrotic and apoptotic cell death were investigated. AgNPs and AgNO3 most
strongly affected the inflamed co-culture. Higher concentrations of AgNPs induced a
significant increase in barrier integrity in the inflamed but not the stable co-
culture. Necrotic and apoptotic cell death was detected in both conditions but were
significantly more pronounced in the inflamed condition. The exposure to AgNO3
affected barrier integrity in all experimental set-ups, but caused nuclear
condensation only in the Caco-2 monoculture and the inflamed co-culture. AgNPs
reduced the release of monocyte chemoattractant protein-1 in the stable model.
Clear differences were observed in the effects of AgNPs and AgNO3 in relation to
the model's health status. The results suggest an increased vulnerability of the
inflamed epithelial barrier towards AgNPs underlining the importance to consider
the intestinal health status in the safety assessment of nanomaterials.
AN - rayyan-553780695
AU - Kampfer, A. A. M.
AU - Urban, P.
AU - La Spina, R.
AU - Jimenez, I. O.
AU - Kanase, N.
AU - Stone, V.
AU - Kinsner-Ovaskainen, A.
DO - 10.1016/j.tiv.2019.104738
KW - Inflammation
Intestines
PY - 2020
SN - 0887-2333
ST - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
Application of an in vitro co-culture model of the healthy and inflamed intestine
TI - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
VL - 63
Y2 - 3
ID - 8718
ER -
TY - JOUR
AB - Chronic inflammatory conditions can negatively impact intestinal barrier
function and affect the epithelium's interaction with nano-sized materials. We
demonstrate the application of a Caco-2/THP-1 co-culture mimicking the intestine in
healthy (i.e. stable) or inflamed state in nanotoxicological research. The co-
cultures were exposed to non-toxic concentrations of silver nanoparticles (AgNPs)
or silver nitrate (AgNO(3)) for 24 h. The barrier integrity and cytokine release as
well as necrotic and apoptotic cell death were investigated. AgNPs and AgNO(3) most
strongly affected the inflamed co-culture. Higher concentrations of AgNPs induced a
significant increase in barrier integrity in the inflamed but not the stable co-
culture. Necrotic and apoptotic cell death was detected in both conditions but were
significantly more pronounced in the inflamed condition. The exposure to AgNO(3)
affected barrier integrity in all experimental set-ups, but caused nuclear
condensation only in the Caco-2 monoculture and the inflamed co-culture. AgNPs
reduced the release of monocyte chemoattractant protein-1 in the stable model.
Clear differences were observed in the effects of AgNPs and AgNO(3) in relation to
the model's health status. The results suggest an increased vulnerability of the
inflamed epithelial barrier towards AgNPs underlining the importance to consider
the intestinal health status in the safety assessment of nanomaterials.
AN - rayyan-553782155
AU - Kämpfer, A. A. M.
AU - Urbán, P.
AU - La Spina, R.
AU - Jiménez, I. O.
AU - Kanase, N.
AU - Stone, V.
AU - Kinsner-Ovaskainen, A.
DO - 10.1016/j.tiv.2019.104738
KW - Caco-2 Cells
Humans
Inflammation
Intestines
Silver/*toxicity
Silver Nitrate/*toxicity
THP-1 Cells
LA - eng
N1 - European Commission, Joint Research Centre (JRC), Ispra, Italy; Nano-Safety
Research Group, School of Engineering and Physical Sciences, Heriot-Watt
University, Edinburgh EH14 4AS, United Kingdom.; European Commission, Joint
Research Centre (JRC), Ispra, Italy.; European Commission, Joint Research Centre
(JRC), Ispra, Italy.; European Commission, Joint Research Centre (JRC), Ispra,
Italy.; Nano-Safety Research Group, School of Engineering and Physical Sciences,
Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom.; Nano-Safety Research
Group, School of Engineering and Physical Sciences, Heriot-Watt University,
Edinburgh EH14 4AS, United Kingdom.; European Commission, Joint Research Centre
(JRC), Ispra, Italy. Electronic address: agnieszka.kinsner-ovaskainen@ec.europa.eu.
PY - 2020
SP - 104738
ST - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
BIBRA
TI - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
VL - 63
Y2 - 3
ID - 10066
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely used nanoparticles and they are
mainly used in antibacterial and personal care products. In this study, we
evaluated the effect of AgNPs on cell death induction in the murine dendritic cell
line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability
and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-
dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-
dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial
membrane potential. The activation of the intracellular signal transduction pathway
was also observed in cells cultured with AgNPs. Taken together, our data
demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS
generation. This study provides important information about the safety of AgNPs
that may help in guiding the development of nanotechnology applications.
AN - rayyan-553780697
AU - Kang, K.
AU - Jung, H.
AU - Lim, J. S.
DO - 10.4062/biomolther.2012.20.4.399
IS - 4
KW - Cell Death
PY - 2012
SN - 1976-9148 2005-4483
SP - 399-405
ST - Cell Death by Polyvinylpyrrolidine-Coated Silver Nanoparticles is Mediated by
ROS-Dependent Signaling
T2 - BIOMOLECULES & THERAPEUTICS
TI - Cell Death by Polyvinylpyrrolidine-Coated Silver Nanoparticles is Mediated by
ROS-Dependent Signaling
VL - 20
Y2 - 7 y3 - 31
ID - 8720
ER -
TY - JOUR
AB - Nanocrystalline silver (Ag) and Ag containing nanostructure synthesized using
various methods have been studied for their antimicrobial, wound healing, and anti-
inflammatory efficacy. Among these, crystalline silver chloride (AgCl)
nanostructures exhibit desirable properties for biological and biomedical
applications. However, most of them are synthesized using hazardous agents and
organic solvents, which has been limited for application in the biological field. A
simple and environmentally friendly method was demonstrated for AgCl nanoparticles
stabilized with chitosan oligomer (CHI-AgCl NPs) as both a resource of Cl ions and
stabilizing agent with expectations of synergistic effects. The CHI-AgCl NPs
stabilized by the chitosan oligomer had spherical morphology with a mean diameter
of 42 ± 15 nm. Ag ions precipitated as AgCl in presence of Cl ions, which remained
in the protonated amine group after HCl hydrolysis of the chitosan. Moreover, much
of the amine and hydroxyl group bound to the AgCl NPs for growth and stabilization.
These nanoparticles were characterized via various spectroscopic techniques,
including UV–Vis spectrophotometry, X-ray photoelectron spectrometry, X-ray
diffractometry, and transmission electron microscopy. © 2014, Springer
Science+Business Media New York.
AN - rayyan-553780698
AU - Kang, Y. O.
AU - Lee, T. S.
AU - Park, W. H.
DO - 10.1007/s10856-014-5294-1
IS - 12
Apoptosis
Bacterial Physiological Phenomena
Cell Survival
Chitosan
Excipients
Materials Testing
Metal Nanoparticles
Particle Size
Silver Compounds
Surface Properties
Biohazards
Chlorine
Chlorine compounds
Green Synthesis
Ions
Microorganisms
Nanocrystals
Nanoparticles
Oligomers
Tissue regeneration
X ray diffraction analysis
X rays
antiinfective agent
chitosan
chloride ion
hydroxyl group
nanoparticle
oligomer
silver chloride
silver chloride nanoparticle
unclassified drug
excipient
metal nanoparticle
silver derivative
Nanocrystalline silver
Silver chloride nanoparticles
Spectroscopic technique
Spherical morphologies
VIS spectrophotometry
X-ray photoelectron spectrometries
absorption
Article
controlled study
decomposition
drug synthesis
Escherichia coli
green chemistry
hydrolysis
nonhuman
proton transport
reaction time
spectrophotometry
thermostability
X ray photoelectron spectroscopy
apoptosis
bacterial phenomena and functions
cell survival
chemistry
dose response
drug effects
materials testing
particle size
physiology
procedures
surface property
synthesis
ultrastructure
Silver halides
PY - 2014
SP - 2629-2638
ST - Green synthesis and antimicrobial activity of silver chloride nanoparticles
stabilized with chitosan oligomer
T2 - Journal of Materials Science: Materials in Medicine
TI - Green synthesis and antimicrobial activity of silver chloride nanoparticles
stabilized with chitosan oligomer
84912151045&doi=10.1007%2fs10856-014-5294-
1&partnerID=40&md5=36422bd3023f57ccf5d49e275c6667aa
VL - 25
ID - 8721
ER -
TY - JOUR
AB - OBJECTIVE: The hypothesis that sleeve gastrectomy (SG) is not associated with
an increase in mucosal colorectal cancer (CRC) biomarkers, unlike Roux-en-Y gastric
bypass (RYGB), was tested. DESIGN AND METHODS: Rectal mucosa, blood, and urine were
obtained from morbidly obese patients (n = 23) before and after (median 28 months)
SG, as well as from nonobese controls (n = 20). Rectal epithelial cell mitosis and
apoptosis, crypt size/fission, and pro-inflammatory gene expression were measured,
as well as systemic inflammatory biomarkers, including C-reactive protein (CRP).
RESULTS: The mean pre-operative body mass index in SG patients was 65.7 kg/m2 (24.7
kg/m2 in controls). Mean excess weight loss post-SG was 38.2%. There was a
significant increase in mitosis frequency, crypt size, and crypt fission (all P <
0.01) in SG patients versus controls, as well as evidence of a chronic inflammatory
state (raised CRP and mononuclear cell p65 NFκB binding), but there was no
significant change in these biomarkers after SG, except CRP reduction. Macrophage
migration inhibitory factor mRNA levels were increased by 39% post-SG (P = 0.038).
CONCLUSIONS: Mucosal biomarkers of CRC risk do not increase at 6 months following
SG, unlike RYGB. Biomarkers of rectal crypt proliferation and systemic inflammation
are increased in morbidly obese patients compared with controls.
AN - rayyan-553782411
AU - Kant, P.
AU - Perry, S. L.
AU - Dexter, S. P.
AU - Race, A. D.
AU - Loadman, P. M.
AU - Hull, M. A.
DO - 10.1002/oby.20493
IS - 1
KW - Adolescent
Adult
Aged
Body Mass Index
C-Reactive Protein/metabolism
Cell Proliferation
Colorectal Neoplasms/*surgery
Female
Gastrectomy/*methods
Gastric Bypass/*methods
Humans
Inflammation
Interleukin-6/blood
Leukocytes, Mononuclear/metabolism
Macrophage Migration-Inhibitory Factors/genetics/metabolism
Male
Middle Aged
Obesity, Morbid/blood/*surgery
RNA, Messenger/genetics/metabolism
Treatment Outcome
Tumor Necrosis Factor-alpha/blood
Weight Loss
Young Adult
Biological Markers
Gastrectomy
LA - eng
PY - 2014
SP - 202-10
ST - Mucosal biomarkers of colorectal cancer risk do not increase at 6 months
following sleeve gastrectomy, unlike gastric bypass
TI - Mucosal biomarkers of colorectal cancer risk do not increase at 6 months
following sleeve gastrectomy, unlike gastric bypass
VL - 22
Y2 - 1
ID - 10318
ER -
TY - JOUR
AB - Development of novel antidiabetic agents using various organic compounds and
biomolecules has been in practice for a longtime. Recently, nanomaterials are also
being used in antidiabetic studies for their unique properties such as small size,
biocompatibility and ability to penetrate cell membrane for carrying drugs. Herein,
in vivo antidiabetic activity of gold nanoparticles (AuNPs) synthesized using the
antidiabetic potent plant Gymnema sylvestre R. Br on wistar albino rats has been
evaluated. The formation of AuNPs and their morphology were confirmed using
spectroscopic and microscopic analyses, respectively. The treatment of AuNPs has
shown significant reduction in blood glucose level on diabetic rats. AuNPs were
also tested for its anti-inflammatory effect by estimating the serum levels of
tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and high-sensitive C-
reactive protein (CRP). (C) 2014 Elsevier B.V. All rights reserved.
AN - rayyan-553780701
AU - Karthick, V.
AU - Kumar, V. G.
AU - Dhas, T. S.
AU - Singaravelu, G.
AU - Sadiq, A. M.
AU - Govindaraju, K.
DO - 10.1016/j.colsurfb.2014.07.022
KW - Rats
PY - 2014
SN - 0927-7765 1873-4367
SP - 505-511
ST - Effect of biologically synthesized gold nanoparticles on alloxan-induced
diabetic rats-An in vivo approach
TI - Effect of biologically synthesized gold nanoparticles on alloxan-induced
diabetic rats-An in vivo approach
VL - 122
Y2 - 10 y3 - 1
ID - 8724
ER -
TY - JOUR
AB - Nanoparticles (NPs) are currently being investigated along with the use of
biodegradable polymer containing active agents in many areas of medicine for
targeted applications. The present study was aimed to synthesize novel compound
Benzodioxane midst piperazine (BP) and characterization of a BP decorated chitosan
silver nanoparticles (BP*C@AgNPs) and shown effective against hazardous pathogens,
and also having anti-inflammatory property. It was further evaluated for molecular
docking proofs, and toxicity. The BP*C@AgNPs had spherical shape with size of 36.6
nm with wide biocidal activity against hazardous Gram-positive and Gram-negative
bacteria with excellent inhibition at 100 μg/mL for S. aureus (10.08 ± 0.05 mm
ZOI), and E. coli (10.03 ± 0.04 mm ZOI) compared to antibiotic Streptomycin. The
anti-inflammatory activity exhibited IC50 value of 71.61 ± 1.05 μg/mL for
BP*C@AgNPs compared to indomethacin (IC50 = 40.15 ± 1.21 μg/mL). Also, the docking
study of BP showed excellent score for COX1 and DNA gyrase. This in silico study
confirmed the achieved efficacy of BP, with less toxicity against normal PMBCs in
vitro and in vivo studies. This study concludes that, the novel synthesized
BP*C@AgNPs had excellent biocidal property and as anti-inflammatory candidate
revealed by docking studies, it confirms BP*C@AgNPs for first-class therapeutic
applications in the area of medicinal nanotechnology for the coming days. © 2017
AN - rayyan-553780702
AU - Karthik, C. S.
AU - Manukumar, H. M.
AU - Ananda, A. P.
AU - Nagashree, S.
AU - Rakesh, K. P.
AU - Mallesha, L.
AU - Qin, H. L.
AU - Umesha, S.
AU - Mallu, P.
AU - Krishnamurthy, N. B.
DO - 10.1016/j.ijbiomac.2017.12.045
Chitosan
Molecular-docking
Piperazine
Toxicity
Animals
Bacteria
Metal Nanoparticles
Mice
Molecular Conformation
Molecular Docking Simulation
Molecular Dynamics Simulation
Oxidative Stress
Piperazines
Silver
X-Ray Diffraction
benzodiazepine
chitosan
cyclooxygenase 1 inhibitor
gyrase inhibitor
indometacin
piperazine
silver nanoparticle
streptomycin
stromal cell derived factor 1
unclassified drug
[4(4 methoxybenzoyl)piperazin 1 yl] (2,3-dihydrobenzo[b][1,4]dioxin 3 yl)methanone
antiinfective agent
metal nanoparticle
piperazine derivative
silver
animal cell
animal experiment
Article
Bacillus cereus
biosafety
computer model
controlled study
cytotoxicity
drug efficacy
drug synthesis
Escherichia coli
IC50
in vitro study
in vivo study
molecular docking
mouse
nanomedicine
nonhuman
particle size
peripheral blood mononuclear cell
Salmonella enterica serovar Typhimurium
Shigella flexneri
animal
bacterium
chemistry
conformation
drug effect
molecular dynamics
oxidative stress
ultrastructure
X ray diffraction
PY - 2018
SP - 489-502
ST - Synthesis of novel benzodioxane midst piperazine moiety decorated chitosan
silver nanoparticle against biohazard pathogens and as potential anti-inflammatory
candidate: A molecular docking studies
TI - Synthesis of novel benzodioxane midst piperazine moiety decorated chitosan
silver nanoparticle against biohazard pathogens and as potential anti-inflammatory
candidate: A molecular docking studies
85037647743&doi=10.1016%2fj.ijbiomac.2017.12.045&partnerID=40&md5=e8c1c8987e33ad732
f6dd912d619301a
VL - 108
ID - 8725
ER -
TY - JOUR
AB - Natural products are important leads in drug discovery. The search for
effective plant-derived agents or their synthetic analogues has continued to be of
interest to biologists and chemists for a long time. Herein, we have synthesized a
novel compound, P1C, and P1C-Tit*CAgNPs from chitosan; P1C is a precursor and an
anti-inflammatory candidate, which has been validated by molecular docking studies.
The synthesized P1C-Tit*CAgNPs showed monodisperse, spherical, and cationic nature
and antioxidant properties, protecting destabilization of the erythrocyte membrane
by the azo compound 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH); the
involvement of NPs as a protective agent for biomolecules, such as DNA and protein,
followed by the treatment of NPs with AAPH was confirmed. The inhibition of
cellular damage and leakage of cellular inflammatory agents was confirmed by AFM,
SEM, TEM, SDS-PAGE, LDH, and PLA2 enzyme inhibition via in vitro studies. The anti-
inflammatory property of P1C was further validated by in silico molecular docking
studies and showed that, the P1C best pose aligned to PLA2 compared to standard
drug. The significant anticancer property of P1C-Tit*CAgNPs was confirmed against
MCF7, U373, and C6 cancer cell lines. Thus, the present study highlights the
synthesized P1C in P1C-Tit*CAgNPs as a target PLA2-specific anti-inflammatory
candidate, and further tuning of small and development-functionalized nanoparticles
has a great future in medicine; hence, their clinical applications are warranted.
AN - rayyan-553780703
AU - Karthik, C. S.
AU - Manukumar, H. M.
AU - Sandeep, S.
AU - Sudarshan, B. L.
AU - Nagashree, S.
AU - Mallesha, L.
AU - Rakesh, K. P.
AU - Sanjay, K. R.
AU - Mallu, P.
AU - Qin, H. L.
DO - 10.1039/c7md00628d
IS - 4
KW - Piperazines
PY - 2018
SN - 2040-2503 2040-2511
SP - 713-724
ST - Development of piperazine-1-carbothioamide chitosan silver nanoparticles
(P1C-Tit*CAgNPs) as a promising anti-inflammatory candidate: a molecular docking
validation
T2 - MEDCHEMCOMM
TI - Development of piperazine-1-carbothioamide chitosan silver nanoparticles
(P1C-Tit*CAgNPs) as a promising anti-inflammatory candidate: a molecular docking
validation
VL - 9
Y2 - 4 y3 - 1
ID - 8726
ER -
TY - JOUR
AB - Apoptosis induction is a common therapeutic approach. However, many cancer
cells are resistant to apoptotic death and alternative cell death pathways
including pyroptosis and necroptosis need to be triggered. At the same time, danger
signals that include HMGB1 and HSP70 can be secreted/released by damaged cancer
cells that boost antitumor immunity. We studied the cytotoxic effects of AgAu NPs,
Ag NPs and Au NPs with regard to the programmed cell death (apoptosis, necroptosis,
pyroptosis) and the secretion/release of HSP70 and HMGB1. Cancer cell lines were
incubated with 30, 40 and 50 µg/mL of AgAu NPs, Ag NPs and Au NPs. Cytotoxicity was
estimated using the MTS assay, and mRNA fold change of CASP1, CASP3, BCL-2, ZPB1,
HMGB1, HSP70, CXCL8, CSF1, CCL20, NLRP3, IL-1β and IL-18 was used to investigate
the associated programmed cell death. Extracellular levels of HMGB1 and IL-1β were
investigated using the ELISA technique. The nanoparticles showed a dose dependent
toxicity. Pyroptosis was triggered for LNCaP and MDA-MB-231 cells, and necroptosis
for MDA-MB-231 cells. HCT116 cells experience apoptotic death and show increased
levels of extracellular HMGB1. Our results suggest that in a manner dependent of
the cellular microenvironment, AgAu NPs trigger mixed programmed cell death in P53
deficient MDA-MB-231 cells, while they also trigger IL-1β release in MDA-MB-231 and
LNCaP cells and release of HMGB1 in HCT116 cells. © 2022 by the authors. Licensee
AN - rayyan-553780706
AU - Katifelis, H.
AU - Nikou, M. P.
AU - Mukha, I.
AU - Vityuk, N.
AU - Lagopati, N.
AU - Piperi, C.
AU - Farooqi, A. A.
AU - Pippa, N.
AU - Efstathopoulos, E. P.
AU - Gazouli, M.
DO - 10.3390/cancers14061546
IS - 6
KW - Apoptosis
Bimetallic nanoparticles
Gold
Necroptosis
Pyroptosis
Silver
caspase 3
complementary DNA
cryopyrin
gold nanoparticle
interleukin 18
interleukin 1beta
interleukin 1beta converting enzyme
interleukin 8
messenger RNA
protein bcl 2
protein p53
silver nanoparticle
tryptophan
zinc binding protein
apoptosis
Article
cancer cell
cancer cell line
cell culture
cell death
cell line
cell viability
cytotoxicity
fibroblast
GBM2 cell line
gene expression
HCT 116 cell line
HEK293 cell line
human
human cell
immunity
inflammation
Li-Fraumeni syndrome
LNCaP C4-2B cell line
LNCaP cell line
malignant neoplasm
MCF-7 cell line
MTS assay
necroptosis
pyroptosis
RNA extraction
Humanities
Humanism
Humans
Aged
Aging
PY - 2022
ST - Ag/Au Bimetallic Nanoparticles Trigger Different Cell Death Pathways and
Affect Damage Associated Molecular Pattern Release in Human Cell Lines
T2 - Cancers
TI - Ag/Au Bimetallic Nanoparticles Trigger Different Cell Death Pathways and
Affect Damage Associated Molecular Pattern Release in Human Cell Lines
85126549212&doi=10.3390%2fcancers14061546&partnerID=40&md5=f1004ede8b4bd9acea12b5b2
90530a36
VL - 14
ID - 8729
ER -
TY - JOUR
AB - The hydrazones of nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac
and ibuprofen are synthesized with aldehydes of pyridine and imidazole and are
characterized by H-1, C-13 and mass spectroscopy. Cu(II) complexes of hydrazones
constructed from these ligands possess square planar geometry for bidentate
diclofenac-hydrazone and tridentate ibuprofen-hydrazone conjugates with [Cu(L)(2)]
and [Cu(L)Cl] compositions, respectively. The observed irreversible Cu(II)/Cu(I)
redox couple in the range of +0.20 to +0.61 V is due to the substantial distortion
in the square-planar geometry. ESR studies indicate the appreciably covalent
character within M-L bonding due to extensive delocalization of electron from d (x)
(2) (--) (y) (2) orbital. The hydrazone-NSAID conjugates exhibit substantial
cytotoxicity against A-549, HCT-116 and MDA-MB-231 cancer cell lines with
ibuprofen-imidazole-hydrazone ligand possessing the lowest IC50 (2.26 mu M) amongst
the synthesized NSAID-conjugates. Interestingly, its Cu(II) complex also displays
excellent anticancer activity against MDA-MB- 231 with IC50 value of 3.58 mu M.
Such a feature may be ascribed to the synergistic association of Cu(II)-NSAID-
hydrazone linkage. Thus, conjugation of NSAID with hydrazone and its complexation
with a bioactive metal ion may be regarded as a potential strategy for designing of
non-platinum anticancer agents.
AN - rayyan-553780708
AU - Kaur, J.
AU - Chikate, T.
AU - Bandyopadhyay, P.
AU - Basu, S.
AU - Chikate, R.
DO - 10.1080/00958972.2020.1843160
IS - 23
KW - Hydrazones
PY - 2020
SN - 0095-8972 1029-0389
SP - 3186-3202
ST - Cu(II) complexes of hydrazones-NSAID conjugates: synthesis, characterization
and anticancer activity
T2 - JOURNAL OF COORDINATION CHEMISTRY
TI - Cu(II) complexes of hydrazones-NSAID conjugates: synthesis, characterization
and anticancer activity
VL - 73
Y2 - 11 y3 - 11
ID - 8731
ER -
TY - CONF
AN - rayyan-553780709
AU - Kawamori, T.
AU - Nakatsugi, S.
AU - Ohta, T.
AU - Sugimura, T.
AU - Wakabayashi, K.
DA - 2002
DO - 10.1007/978-1-4615-0193-0_57
KW - Animals
Anticarcinogenic Agents
Carcinogens
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors
Female
Imidazoles
Isoenzymes
Mammary Neoplasms, Animal
Prostaglandin-Endoperoxide Synthases
Rats
Sulfonamides
Animalia
2 amino 1 methyl 6 phenylimidazo[4,5 b]pyridine
cyclooxygenase 2 inhibitor
cytokine
DNA fragment
growth factor
lipopolysaccharide
nimesulide
prostaglandin E2
2 amino 1 methyl 6 phenylimidazo(4,5 b)pyridine
2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
carcinogen
cyclooxygenase 2
imidazole derivative
isoenzyme
prostaglandin synthase
prostaglandin synthase inhibitor
sulfonamide
animal experiment
animal model
animal tissue
apoptosis
body weight
breast carcinogenesis
breast carcinoma
cell proliferation
chemoprophylaxis
conference paper
controlled study
drug mechanism
drug selectivity
enzyme activity
enzyme induction
female
fibroblast
hormone determination
inflammation
macrophage
mitogenesis
nonhuman
priority journal
protein expression
protein function
rat
silver staining
tumor growth
Western blotting
animal
article
chemically induced disorder
drug effect
experimental neoplasm
metabolism
pathology
Chemoprevention
SP - 371-376
ST - Chemopreventive effects of nimesulide, a selective cyclooxygenase-2
inhibitor, against PHIP-induced mammary carcinogenesis
T2 - Advances in Experimental Medicine and Biology
TI - Chemopreventive effects of nimesulide, a selective cyclooxygenase-2
inhibitor, against PHIP-induced mammary carcinogenesis
0142226855&doi=10.1007%2f978-1-4615-0193-
0_57&partnerID=40&md5=0c5e44b59de0a0b61e5199d3d8ee2556
VL - 507
ID - 8732
ER -
TY - JOUR
AB - The interest in the application of plant extracts as modifiers of polymers
intended for biomedical purposes is constantly increasing. The therapeutical
properties of the licorice root, including its anti-inflammatory and antibacterial
activity, make this plant particularly promising. The same applies to silver
nanoparticles showing antibacterial properties. Thus the main purpose of the
research was to design hydrogel dressings containing both licorice root extract and
nanosilver so as to obtain a system promoting wound regeneration processes by
preventing infection and inflammation within the wound. The first step included the
preparation of the plant extract via the solid-liquid extraction using the Soxhlet
extractor and the synthesis of silver nanoparticles by the chemical reduction of
silver ions using a sodium borohydride as a reducing agent. Subsequently, hydrogels
were synthesized via photopolymerization and subjected to studies aiming at
characterizing their sorption properties, surface morphology via scanning electron
microscopy, and their impact on simulated physiological liquids supported by
defining these liquids' influence on hydrogels' structures by FT-IR spectroscopy.
Next, the tensile strength of hydrogels and their percentage elongation were
determined. Performed studies also allowed for determining the hydrogels'
wettability and free surface energies. Finally, the cytotoxicity of hydrogels
towards L929 murine fibroblasts via the MTT reduction assay was also verified. It
was demonstrated that developed materials showed stability in simulated
physiological liquids. Moreover, hydrogels were characterized by high elasticity
(percentage elongation within the range of 24-29%), and their surfaces were
hydrophilic (wetting angles below 90 degrees). Hydrogels containing both licorice
extract and nanosilver showed smooth and homogeneous surfaces. Importantly,
cytotoxic properties towards L929 murine fibroblasts were excluded; thus, developed
materials seem to have great potential for application as innovative dressings.
AN - rayyan-553780710
AU - Kedzierska, M.
AU - Bankosz, M.
AU - Drabczyk, A.
AU - Kudlacik-Kramarczyk, S.
AU - Jamrozy, M.
AU - Potemski, P.
DO - 10.3390/ijms24010217
IS - 1
KW - Hydrogel
Hydrogels
Bandages
PY - 2023
SN - 1422-0067
ST - Silver Nanoparticles and Glycyrrhiza glabra (Licorice) Root Extract as
Modifying Agents of Hydrogels Designed as Innovative Dressings
TI - Silver Nanoparticles and Glycyrrhiza glabra (Licorice) Root Extract as
Modifying Agents of Hydrogels Designed as Innovative Dressings
VL - 24
Y2 - 1
ID - 8733
ER -
TY - JOUR
AB - The interest in magnetic nanoparticles is constantly growing, which is due to
their unique properties, of which the most useful is the possibility of directing
their movement via an external magnetic field. Thus, applications may be found for
them as carriers in targeted drug delivery. These nanomaterials usually form a core
in a core–shell structure, and a shell may be formed via various compounds. Here,
nanosilver-shelled iron oxide magnetic nanoparticles were developed. Various
reaction media and various Arabic gum (stabilizer) solution concentrations were
investigated to verify those that were most beneficial one in limiting their
agglomeration as much as possible. The essential oil of lavender was proposed as a
component of such a medium; it was used alone or in combination with distilled
water as a solvent of the stabilizer. The particle size was characterized by
dynamic light scattering (DLS), the chemical structure was characterized via FT-IR
spectroscopy, the crystallinity was characterized by X-ray diffraction (XRD), and
the surface morphology and elemental composition were verified via the SEM-EDS
technique. Moreover, UV-Vis spectrophotometry was used to verify the presence of
the shell made of nanosilver. Importantly, the particles’ pro-inflammatory activity
and cytotoxicity towards L929 murine fibroblasts were also characterized. It was
demonstrated that a 3% stabilizer solution provided a preparation of Fe3O4@Ag
particles, but its stabilizing effect was not sufficient, as a suspension with
micrometric particles was obtained; thus it was necessary to apply 4 h of
sonication for their crushing. Next, the oil/water reaction medium was verified as
beneficial in terms of nanoparticle formation. In such reaction conditions, the
formation of particle agglomerates was strongly limited, and after 15 min of
sonication a suspension containing only nanoparticles was obtained. The presence of
a nanosilver shell was confirmed spectrophotometrically via XRD and SEM-EDS
techniques. Importantly, the developed nanomaterials showed no cytotoxicity towards
murine fibroblasts and no pro-inflammatory activity. © 2022 by the authors.
Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780711
AU - Kędzierska, M.
AU - Drabczyk, A.
AU - Jamroży, M.
AU - Kudłacik-Kramarczyk, S.
AU - Głąb, M.
AU - Potemski, P.
AU - Tyliszczak, B.
DO - 10.3390/ma15124050
IS - 12
KW - arabic gum
colloidal stability
core–shell nanostructures
iron oxide magnetic nanoparticles
pro-inflammatory activity
ultrasound-assisted agglomerate crushing
Cell culture
Cell proliferation
Crystallinity
Cytotoxicity
Dynamic light scattering
Magnetite
Morphology
Particle size
Particle size analysis
Sols
Sonication
Surface morphology
Suspensions (fluids)
Ultrasonic applications
X ray diffraction
Arabic gums
Colloidal Stability
Core shell nano structures
Core-shell nanostructures
Inflammatory activity
Iron oxide magnetic nanoparticle
Nano silver
Pro-inflammatory activity
Stabiliser
Ultrasound-assisted agglomerate crushing
Oxalic Acid
Iron
Norisoprenoids
PY - 2022
ST - Iron Oxide Magnetic Nanoparticles with a Shell Made from Nanosilver—Synthesis
Methodology and Characterization of Physicochemical and Biological Properties
T2 - Materials
TI - Iron Oxide Magnetic Nanoparticles with a Shell Made from Nanosilver—Synthesis
Methodology and Characterization of Physicochemical and Biological Properties
85132132518&doi=10.3390%2fma15124050&partnerID=40&md5=ab9a386c5ccc6ec29c4e74b682906
be0
VL - 15
ID - 8734
ER -
TY - JOUR
AB - Chronic non-healing wounds represent a substantial economic burden to
healthcare systems and cause a considerable reduction in quality of life for those
affected. Approximately 0.5-2% of the population in developed countries are
projected to experience a chronic wound in their lifetime, necessitating further
developments in the area of wound care materials. The use of aerogels for wound
healing applications has increased due to their high exudate absorbency and ability
to incorporate therapeutic substances, amongst them trace metals, to promote wound-
healing. This study evaluates the swelling behavior of Ca-Zn-Ag-loaded alginate
aerogels and their metal release upon incubation in human sweat or wound fluid
substitutes. All aerogels show excellent liquid uptake from any of the formulas and
high liquid holding capacities. Calcium is only marginally released into the
swelling solvents, thus remaining as alginate bridging component aiding the
absorption and fast transfer of liquids into the aerogel network. The zinc transfer
quota is similar to those observed for common wound dressings in human and animal
injury models. With respect to the immune regulatory function of zinc, cell culture
studies show a high availability and anti-inflammatory activity of aerogel released
Zn-species in RAW 264.7 macrophages. For silver, the balance between antibacterial
effectiveness versus cytotoxicity remains a significant challenge for which the
alginate aerogels need to be improved in the future. An increased knowledge of the
transformations that alginate aerogels undergo in the course of the fabrication as
well as during wound fluid exposure is necessary when aiming to create advanced,
tissue-compatible aerogel products.
AN - rayyan-553780713
AU - Keil, C.
AU - Hubner, C.
AU - Richter, C.
AU - Lier, S.
AU - Barthel, L.
AU - Meyer, V.
AU - Subrahmanyam, R.
AU - Gurikov, P.
AU - Smirnova, I.
AU - Haase, H.
DO - 10.3390/polym12112741
IS - 11
KW - Humanities
Humanism
Humans
Wound Healing
Body Fluids
Macrophages
PY - 2020
SN - 2073-4360
ST - Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior
in Simulated Human Body Fluids and Effect on Macrophages
T2 - POLYMERS
TI - Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior
VL - 12
Y2 - 11
ID - 8736
ER -
TY - JOUR
AB - Chronic non-healing wounds represent a substantial economic burden to
healthcare systems and cause a considerable reduction in quality of life for those
affected. Approximately 0.5-2% of the population in developed countries are
projected to experience a chronic wound in their lifetime, necessitating further
developments in the area of wound care materials. The use of aerogels for wound
healing applications has increased due to their high exudate absorbency and ability
to incorporate therapeutic substances, amongst them trace metals, to promote wound-
healing. This study evaluates the swelling behavior of Ca-Zn-Ag-loaded alginate
aerogels and their metal release upon incubation in human sweat or wound fluid
substitutes. All aerogels show excellent liquid uptake from any of the formulas and
high liquid holding capacities. Calcium is only marginally released into the
swelling solvents, thus remaining as alginate bridging component aiding the
absorption and fast transfer of liquids into the aerogel network. The zinc transfer
quota is similar to those observed for common wound dressings in human and animal
injury models. With respect to the immune regulatory function of zinc, cell culture
studies show a high availability and anti-inflammatory activity of aerogel released
Zn-species in RAW 264.7 macrophages. For silver, the balance between antibacterial
effectiveness versus cytotoxicity remains a significant challenge for which the
alginate aerogels need to be improved in the future. An increased knowledge of the
transformations that alginate aerogels undergo in the course of the fabrication as
well as during wound fluid exposure is necessary when aiming to create advanced,
tissue-compatible aerogel products.
AN - rayyan-553782308
AU - Keil, C.
AU - Hübner, C.
AU - Richter, C.
AU - Lier, S.
AU - Barthel, L.
AU - Meyer, V.
AU - Subrahmanyam, R.
AU - Gurikov, P.
AU - Smirnova, I.
AU - Haase, H.
DO - 10.3390/polym12112741
IS - 11
J2 - Polymers (Basel)
KW - Humanities
Humanism
Humans
Wound Healing
Body Fluids
Macrophages
LA - eng
N1 - Department Food Chemistry and Toxicology, Institute of Food Technology and
Food Chemistry, TU Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.;
Department Food Chemistry and Toxicology, Institute of Food Technology and Food
Chemistry, TU Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.; Department
Food Chemistry and Toxicology, Institute of Food Technology and Food Chemistry, TU
Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.; Department Food Chemistry
and Toxicology, Institute of Food Technology and Food Chemistry, TU Berlin, Straße
des 17. Juni 135, 10623 Berlin, Germany.; Applied and Molecular Microbiology,
Institute of Biotechnology, TU Berlin, Straße des 17. Juni 135, 10623 Berlin,
Germany.; Applied and Molecular Microbiology, Institute of Biotechnology, TU
Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.; Institute of Thermal
Separation Processes, Hamburg University of Technology, Eißendorfer Straße 38,
21073 Hamburg, Germany.; Laboratory for Development and Modelling of Novel
Nanoporous Materials, Hamburg University of Technology, Eißendorfer Straße 38,
21073 Hamburg, Germany.; Institute of Thermal Separation Processes, Hamburg
University of Technology, Eißendorfer Straße 38, 21073 Hamburg, Germany.;
Department Food Chemistry and Toxicology, Institute of Food Technology and Food
Chemistry, TU Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
PY - 2020
ST - Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior
T2 - Polymers
TI - Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior
VL - 12
Y2 - 11 y3 - 18
ID - 10216
ER -
TY - JOUR
AB - Engineered nanoparticles are increasingly used in medical applications and
day-to-day consumer products, leading to concerns about the potential environmental
and human health impacts. Silver nanoparticles are particularly prevalent because
of their use as antibacterial agents in many commonly available products.
Nanoparticles (NPs) are believed to accumulate, often preferentially, in the liver.
This study therefore investigates the effect of a silver NP (20 nm) on the liver,
and in particular, the role of Kupffer cells (KCs; resident liver macrophages) in
the overall inflammatory response in the organ. Cytokine expression in the normal
liver was measured in terms of IL2, IL4, TNF-alpha, IFN-gamma and IL10 released
from the organ with significant up-regulation of TNF-alpha and IL10 being observed.
For livers in which the KC population was specifically targeted and destroyed this
cytokine increase was significantly decreased in comparison to the normal tissue.
IL10 was secreted at approximately three times the concentration of TNF-alpha in
all the test cases. The high levels of IL10 released from the normal tissue in
comparison to the KC depleted livers suggest that the cytokine may help to protect
against a pro-inflammatory response to these Ag NPs. This may indicate a
potentially important role for KCs in the anti-inflammatory response and suggests
that tolerance to the Ag NPs is favoured over a fully activated immune response. In
addition, albumin production was measured as an indicator of hepatic function. It
was noted that the liver function was unaffected by the Ag NPs.
AN - rayyan-553780714
AU - Chauche, C.
AU - Balharry, D.
AU - Brown, D. M.
AU - Kanase, N.
AU - Boczkowski, J.
AU - Lanone, S.
AU - Stone, V.
DO - 10.3109/17435390.2013.866284
PY - 2014
SN - 1743-5390 1743-5404
SP - 149-154
ST - The role of Kupffer cells in the hepatic response to silver nanoparticles
T2 - NANOTOXICOLOGY
TI - The role of Kupffer cells in the hepatic response to silver nanoparticles
VL - 8
Y2 - 8
ID - 8737
ER -
TY - JOUR
AB - The use of hepatocyte cell lines as a replacement for animal models have been
heavily criticised mainly due to low expression of metabolism enzymes. This study
compares primary human hepatocytes with the C3A cell line and with respect to their
response to a panel of nanomaterials (NMs; two ZnO, two MWCNTs, one Ag and one
positively functionalised TiO2). The cell line was very comparable with the primary
hepatocytes with regards to their cytotoxic response to the NMs (Ag > uncoated ZnO
> coated ZnO). The LC50 was not attained in the presence of the MWCNTs and the TiO2
NMs. All NMs significantly increased IL-8 production, with no change in levels of
TNF-α and IL-6. Albumin production was measured as an indicator of hepatic
function. The authors found no change in levels of albumin with the exception of
the coated ZnO NM at the LC50 concentration. NM uptake was similar for both the
primary hepatocytes and C3A cells as investigated by TEM. Meanwhile, the authors
confirmed greater levels of CYP450 activity in untreated primary cells. This study
demonstrates that the C3A cell line is a good model for investigating NM-induced
hepatocyte responses with respect to uptake, cytotoxicity, pro-inflammatory
cytokine production and albumin production. © Informa UK, Ltd.
AN - rayyan-553780715
AU - Gaiser, B. K.
AU - Ward, M. B.
AU - Stone, V.
DO - 10.3109/17435390.2012.734341
IS - 7
KW - Cytokine production
Cytotoxicity
Liver
Nanomaterial uptake
Albumins
Animal Testing Alternatives
Cell Line, Tumor
Cell Survival
Cytokines
Endocytosis
Hepatocytes
Humans
Lethal Dose 50
Nanoparticles
Particle Size
Surface Properties
Toxicity Tests
albumin
interleukin 6
interleukin 8
nanomaterial
silver
titanium dioxide
zinc oxide
article
cancer cell culture
cell viability
controlled study
cytokine production
cytotoxicity
human
human cell
in vitro study
LC 50
liver cell
liver function
nanotoxicology
priority journal
Humanities
Humanism
Cell Line
PY - 2013
SP - 1255-1271
ST - Primary human hepatocytes versus hepatic cell line: Assessing their
suitability for in vitro nanotoxicology
T2 - Nanotoxicology
TI - Primary human hepatocytes versus hepatic cell line: Assessing their
suitability for in vitro nanotoxicology
84885145308&doi=10.3109%2f17435390.2012.734341&partnerID=40&md5=fa21a15c424fa31bfde
87d6a6c28b5f4
VL - 7
ID - 8738
ER -
TY - JOUR
AB - Silver (Ag) nanoparticles (NPs) are currently among one of the most widely
used nanomaterials. This in turn, implies an increased risk of human and
environmental exposure. Alcohol abuse is a global issue with millions of people in
the general population affected by the associated adverse effects. The excessive
consumption of alcohol is a prominent cause of chronic liver disease which manifest
in multiple disorders. In this study, the adverse health effects of Ag NP exposure
were investigated in models of alcoholic hepatic disease in vitro and in vivo. The
data showed that Ag NP induced hepatic health effects were aggravated in the
alcohol pretreated mice in comparison to controls with regards to an organ specific
inflammatory response, changes in blood biochemistry, acute phase response and
hepatic pathology. In addition, alcoholic disease influenced the organ's ability
for recovery post-NP challenge. Additionally, it is demonstrated that the in vivo
data correlated well with in vitro findings where ethanol pretreatment of
hepatocytes resulted in significantly increased inflammatory response post-Ag NP
exposure. To the best of our knowledge this is the first study of its kind to
investigate nano-sized material-induced hepatic pathology in models representative
of susceptible individuals (those with pre-existing alcohol liver disease) within
the population. This is an area of research in the field of nanotoxicology, and in
particular with regard to NP risk assessment that is almost entirely overlooked.
AN - rayyan-553782306
AU - Roursgaard, M.
AU - Loft, S.
AU - Møller, P.
DO - 10.1093/toxsci/kfx080
IS - 1
J2 - Toxicol Sci
KW - Acute-Phase Reaction
Animals
Antioxidants/metabolism
Biomarkers/blood
Ethanol/*administration & dosage
Female
Glutathione/metabolism
Hep G2 Cells
Humans
Interleukin-8/biosynthesis
Liver/*drug effects/metabolism
Liver Diseases, Alcoholic/pathology
Mice
Silver/*chemistry
Alcoholics
LA - eng
N1 - Department of Public Health, Section of Environmental Health, University of
Copenhagen, Copenhagen, Denmark.; National Research Centre for the Working
Environment, Copenhagen, Denmark.; Department of Public Health, Section of
Environmental Health, University of Copenhagen, Copenhagen, Denmark.; Department of
Public Health, Section of Environmental Health, University of Copenhagen,
Copenhagen, Denmark.; Department of Public Health, Section of Environmental Health,
University of Copenhagen, Copenhagen, Denmark.
PY - 2017
SP - 176-187
ST - Hepatic Hazard Assessment of Silver Nanoparticle Exposure in Healthy and
Chronically Alcohol Fed Mice
T2 - Toxicological sciences : an official journal of the Society of Toxicology
TI - Hepatic Hazard Assessment of Silver Nanoparticle Exposure in Healthy and
Chronically Alcohol Fed Mice
VL - 158
Y2 - 7 y3 - 1
ID - 10214
ER -
TY - JOUR
AB - Effects on the liver C3A cell line treated with a panel of engineered
nanomaterials (NMs) consisting of two zinc oxide particles (ZnO; coated 100 nm and
uncoated 130 nm), two multi-walled carbon nanotubes (MWCNTs), one silver (Ag < 20
nm), one 7 nm anatase, two rutile TiO2 nanoparticles (10 and 94 nm) and two
derivatives with positive and negative covalent functionalisation of the 10 nm
rutile were evaluated. The silver particles elicited the greatest level of
cytotoxicity (24 h LC50-2 μg/cm2). The silver was followed by the uncoated ZnO (24
h LC50-7.5 μg/cm2) and coated ZnO (24 h LC50-15 μg/cm2) particles with respect to
cytotoxicity. The ZnO NMs were found to be about 50-60% soluble which could account
for their toxicity. By contrast, the Ag was <1% soluble. The LC50 was not attained
in the presence of any of the other engineered NMs (up to 80 μg/cm2). All NMs
significantly increased IL-8 production. Meanwhile, no significant change in TNF-α,
IL-6 or CRP was detected. Urea and albumin production were measured as indicators
of hepatic function. These markers were only altered by the coated and uncoated
ZnO, which significantly decreased albumin production. © 2013 Informa UK, Ltd.
AN - rayyan-553780716
AU - Pojana, G.
AU - Gaiser, B. K.
AU - Birkedal, R.
AU - Bilaničová, D.
AU - Wallin, H.
AU - Jensen, K. A.
AU - Sellergren, B.
AU - Hutchison, G. R.
AU - Marcomini, A.
AU - Stone, V.
DO - 10.3109/17435390.2011.653416
IS - 3
KW - Albumin
IL-8
Inflammation
Liver
Urea
Albumins
Biological Markers
Cell Line, Tumor
Cell Survival
Cytokines
Hepatocytes
Humans
Nanostructures
Silver
Titanium
Zinc Oxide
albumin
biological marker
C reactive protein
interleukin 6
interleukin 8
nanomaterial
silver nanoparticle
titanium dioxide
urea
zinc oxide
article
cell viability
controlled study
cytokine production
cytokine release
cytopathogenic effect
human
human cell
in vitro study
LC 50
liver cell
liver function test
material coating
nanoengineering
priority journal
protein synthesis
solubility
Cell Line
PY - 2013
SP - 301-313
ST - In vitro assessment of engineered nanomaterials using a hepatocyte cell line:
Cytotoxicity, pro-inflammatory cytokines and functional markers
T2 - Nanotoxicology
TI - In vitro assessment of engineered nanomaterials using a hepatocyte cell line:
Cytotoxicity, pro-inflammatory cytokines and functional markers
84876037765&doi=10.3109%2f17435390.2011.653416&partnerID=40&md5=b0ad60bb40d50f6f974
026de9eac8d06
VL - 7
ID - 8739
ER -
TY - JOUR
uncoated 130 nm), two multiwalled carbon nanotubes (MWCNTs), one silver (Ag < 20
cytotoxicity (24 h LC50 - 2 mu g/cm(2)). The silver was followed by the uncoated
ZnO (24 h LC50 - 7.5 mu g/cm(2)) and coated ZnO (24 h LC50 - 15 mu g/cm(2))
particles with respect to cytotoxicity. The ZnO NMs were found to be about 50-60%
soluble which could account for their toxicity. By contrast, the Ag was <1%
soluble. The LC50 was not attained in the presence of any of the other engineered
NMs (up to 80 mu g/cm(2)). All NMs significantly increased IL-8 production.
Meanwhile, no significant change in TNF-alpha, IL-6 or CRP was detected. Urea and
albumin production were measured as indicators of hepatic function. These markers
were only altered by the coated and uncoated ZnO, which significantly decreased
albumin production.
AN - rayyan-553780717
AU - Pojana, G.
AU - Gaiser, B. K.
AU - Birkedal, R.
AU - Bilanicova, D.
AU - Wallin, H.
AU - Jensen, K. A.
AU - Sellergren, B.
AU - Marcomini, A.
AU - Stone, V.
DO - 10.3109/17435390.2011.653416
IS - 3
KW - Cell Line
PY - 2013
SN - 1743-5390 1743-5404
SP - 301-313
cytotoxicity, pro-inflammatory cytokines and functional markers
T2 - NANOTOXICOLOGY
VL - 7
Y2 - 5
ID - 8740
ER -
TY - JOUR
uncoated 130 nm), two multi-walled carbon nanotubes (MWCNTs), one silver (Ag < 20
cytotoxicity (24 h LC50 - 2 µg/cm(2)). The silver was followed by the uncoated ZnO
(24 h LC50 - 7.5 µg/cm(2)) and coated ZnO (24 h LC50 - 15 µg/cm(2)) particles with
respect to cytotoxicity. The ZnO NMs were found to be about 50-60% soluble which
could account for their toxicity. By contrast, the Ag was <1% soluble. The LC50 was
not attained in the presence of any of the other engineered NMs (up to 80
µg/cm(2)). All NMs significantly increased IL-8 production. Meanwhile, no
significant change in TNF-α, IL-6 or CRP was detected. Urea and albumin production
were measured as indicators of hepatic function. These markers were only altered by
the coated and uncoated ZnO, which significantly decreased albumin production.
AN - rayyan-553782041
AU - Pojana, G.
AU - Gaiser, B. K.
AU - Birkedal, R.
AU - Bilanicová, D.
AU - Wallin, H.
AU - Jensen, K. A.
AU - Sellergren, B.
AU - Marcomini, A.
AU - Stone, V.
DO - 10.3109/17435390.2011.653416
IS - 3
J2 - Nanotoxicology
KW - Albumins/metabolism
Biomarkers/metabolism
Cell Line, Tumor
Cytokines/*metabolism
Hepatocytes/cytology/*drug effects/metabolism
Humans
Nanostructures/*toxicity
Silver/toxicity
Titanium/toxicity
Urea/metabolism
Zinc Oxide/toxicity
Cell Line
LA - eng
N1 - Heriot-Watt University, School of Life Sciences, John Muir Building,
Edinburgh, UK. Ak435@hw.ac.uk
PY - 2013
SP - 301-13
T2 - Nanotoxicology
VL - 7
Y2 - 5
ID - 9955
ER -
TY - JOUR
AB - The liver is one of the most important multi-functional organs in the human
body. Amongst various crucial functions, it is the main detoxification center and
predominantly implicated in the clearance of xenobiotics potentially including
particulates that reach this organ. It is now well established that a significant
quantity of injected, ingested or inhaled nanomaterials (NMs) translocate from
primary exposure sites and accumulate in liver. This review aimed to summarize and
discuss the progress made in the field of hepatic nanotoxicology, and crucially
highlight knowledge gaps that still exist. Key considerations include In vivo
studies clearly demonstrate that low-solubility NMs predominantly accumulate in the
liver macrophages the Kupffer cells (KC), rather than hepatocytes. KCs lining the
liver sinusoids are the first cell type that comes in contact with NMs in vivo.
Further, these macrophages govern overall inflammatory responses in a healthy
liver. Therefore, interaction with of NM with KCs in vitro appears to be very
important. Many acute in vivo studies demonstrated signs of toxicity induced by a
variety of NMs. However, acute studies may not be that meaningful due to liver's
unique and unparalleled ability to regenerate. In almost all investigations where a
recovery period was included, the healthy liver was able to recover from NM
challenge. This organ's ability to regenerate cannot be reproduced in vitro.
However, recommendations and evidence is offered for the design of more
physiologically relevant in vitro models. Models of hepatic disease enhance the NM-
induced hepatotoxicity. The review offers a number of important suggestions for the
future of hepatic nanotoxicology study design. This is of great significance as its
findings are highly relevant due to the development of more advanced in vitro, and
in silico models aiming to improve physiologically relevant toxicological testing
strategies and bridging the gap between in vitro and in vivo experimentation.
AN - rayyan-553780718
AU - Powell, L. G.
AU - Stone, V.
DO - 10.1080/10937404.2020.1751756
IS - 4
PY - 2020
SN - 1093-7404 1521-6950
SP - 137-176
ST - A review of hepatic nanotoxicology - summation of recent findings and
considerations for the next generation of study designs
T2 - JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART B-CRITICAL REVIEWS
TI - A review of hepatic nanotoxicology - summation of recent findings and
considerations for the next generation of study designs
VL - 23
Y2 - 5 y3 - 18
ID - 8741
ER -
TY - JOUR
AB - BACKGROUND: It has been shown that nanomaterials (NMs) are able to
translocate to secondary tissues one of the important being the kidneys. Oxidative
stress has been implicated as a possible mechanism for NM toxicity, hence effects
on the human renal proximal tubule epithelial cells (HK-2) treated with a panel of
engineered nanomaterials (NMs) consisting of two zinc oxide particles (ZnO - coated
- NM 110 and uncoated - NM 111), two multi walled carbon nanotubes (MWCNT) (NM 400
and NM 402), one silver (NM 300) and five TiO2 NMs (NM 101, NRCWE 001, 002, 003 and
004) were evaluated. METHODS: In order to assess the toxicological impact of the
engineered NMs on HK-2 cells - WST-1 cytotoxicity assay, FACSArray, HE oxidation
and the comet assays were utilised. For statistical analysis, the experimental
values were compared to their corresponding controls using an ANOVA with Tukey's
multiple comparison. RESULTS: We found the two ZnO NMs (24 hr LC50 - 2.5 μg/cm2)
and silver NM (24 hr LC50 - 10 μg/cm2) were highly cytotoxic to the cells. The LC50
was not attained in the presence of any of the other engineered nanomaterials (up
to 80 μg/cm2). All nanomaterials significantly increased IL8 and IL6 production.
Meanwhile no significant change in TNF-α or MCP-1 was detectable. The most notable
increase in ROS was noted following treatment with the Ag and the two ZnO NMs.
Finally, genotoxicity was measured at sub-lethal concentrations. We found a small
but significant increase in DNA damage following exposure to seven of the ten NMs
investigated (NM 111, NRCWE 001 and NRCWE 003 being the exception) with this
increase being most visible following exposure to Ag and the positively charged
TiO2. CONCLUSIONS: While the NMs could be categorised as low and highly cytotoxic,
sub-lethal effects such as cytokine production and genotoxicity were observed with
some of the low toxicity materials.
AN - rayyan-553782007
AU - Vranic, S.
AU - Boland, S.
AU - Moreau, K.
AU - Baeza-Squiban, A.
AU - Gaiser, B. K.
AU - Andrzejczuk, L. A.
AU - Stone, V.
DO - 10.1186/1471-2369-14-96
J2 - BMC Nephrol
KW - Animals
Biomedical Engineering/*methods
Cattle
Cell Line
Cell Line, Transformed
Cytotoxins/chemistry/*toxicity
DNA Damage/*drug effects/physiology
Humans
*Inflammation Mediators/metabolism
Kidney/drug effects/metabolism
Nanostructures/chemistry/*toxicity
Oxidative Stress/*drug effects/physiology
Reactive Oxygen Species/metabolism
Humanities
Humanism
Oxidative Stress
LA - eng
N1 - Heriot-Watt University, School of Life Sciences, Edinburgh, EH14 4AS, UK.
A.Kermanizadeh@hw.ac.uk
PY - 2013
SP - 96
ST - An in vitro assessment of panel of engineered nanomaterials using a human
renal cell line: cytotoxicity, pro-inflammatory response, oxidative stress and
genotoxicity
T2 - BMC nephrology
TI - An in vitro assessment of panel of engineered nanomaterials using a human
renal cell line: cytotoxicity, pro-inflammatory response, oxidative stress and
genotoxicity
VL - 14
Y2 - 4 y3 - 25
ID - 9923
ER -
TY - JOUR
AB - Healing of equine distal limb wound represents a challenging problem and
occupies a vital place in everyday pathology. Silver nanoparticles (AgNPs) are
attractive materials for many researchers because of their anti-inflammatory,
antibacterial, and wound-healing properties; as such, this study was performed to
investigate the healing efficacy of AgNPs on second-intention wound healing in five
clinically healthy donkeys as compared with silver sulfadiazine. Wound healing was
evaluated clinically (healing rate [%], healing time [days], epithelialization rate
[%]), whereas the antimicrobial effect of AgNPs was evaluated by comparing with
amoxicillin/metronidazole mixture. In addition, angiogenesis, re-epithelialization,
cellular invasion, and scar width were characterized by histopathology and the
immunohistochemical localization of transforming growth factor beta 1 (TGF beta 1)
was assessed, along with the ultrastructure of the AgNPs-treated wounds.
Accelerated wound healing, better cosmetic appearance, and efficient antimicrobial
effect were achieved with AgNPs dressing. Superiority of AgNPs in wound healing was
approved histopathologically by the early granulation tissue formation, higher
epithelialization (%), and reduced scar width. Positive TGF beta 1 immunoreactivity
with macrophage, fibroblast, and endothelial localization was reported during the
inflammatory and the proliferative phase; however, it has reduced during the
remolding phase producing a faster scar-free wound healing. The cytocompatibility
of the AgNPs with fibroblast was demonstrated by the integrity of the cellular and
nuclear membrane with nonfragmented nucleoli, as detected by the ultrastructural
examination. It could be concluded that AgNPs represent a step forward and a
promising alternative dressing in wound healing in donkeys through its anti-
inflammatory, antimicrobial, and favorable cosmetic properties, in addition to its
role in regulation of TGF beta 1 production. (c) 2017 Elsevier Inc. All rights
reserved.
AN - rayyan-553780722
AU - Khafaga, A. F.
AU - Abu-Ahmed, H. M.
AU - El-Khamary, A. N.
AU - Elmehasseb, I. M.
AU - Shaheen, H. M.
DO - 10.1016/j.jevs.2017.11.013
KW - Wound Healing
PY - 2018
SN - 0737-0806 1542-7412
SP - 76-87
ST - Enhancement of Equid Distal Limb Wounds Healing by Topical Application of
T2 - JOURNAL OF EQUINE VETERINARY SCIENCE
TI - Enhancement of Equid Distal Limb Wounds Healing by Topical Application of
VL - 61
Y2 - 2
ID - 8744
ER -
TY - JOUR
AB - Most studies have used in vitro systems to test inflammatory responses of
nanoparticles; these may not reflect the real biological response of body organs.
In fact, certain nanoparticles have provoked opposite effects under in vitro and in
vivo conditions. Current understanding of the biocompatibility of gold
nanoparticles is controversial. We studied the acute (1 day) and sub-chronic (5
days) effects of gold nanoparticles (10 and 50 nm in diameter) on expression of
interleukin-1 beta (IL-1 beta), IL-6 and tumor necrosis factor alpha (TNF-alpha) in
rat liver. Real-time PCR analysis showed that gold nanoparticles of both sizes
significantly increased cytokine gene expression on day 1; this had subsided by day
5. The 50-nm gold nanoparticle produced more severe inflammation than the smaller
gold nanoparticle. These findings indicate a possible biocompatibility of medium-
sized gold nanoparticles, as they caused only a transient increase in
proinflammatory cytokines, followed by normalization during sub-chronic repeated
exposure.
AN - rayyan-553780727
AU - Khan, H. A.
AU - Abdelhalim, M. A. K.
AU - Alhomida, A. S.
AU - Al Ayed, M. S.
DO - 10.4238/2013.November.22.12
IS - 4
KW - Rats
Gene Expression
PY - 2013
SN - 1676-5680
SP - 5851-5857
ST - Transient increase in IL-1 beta, IL-6 and TNF-alpha gene expression in rat
liver exposed to gold nanoparticles
T2 - GENETICS AND MOLECULAR RESEARCH
TI - Transient increase in IL-1 beta, IL-6 and TNF-alpha gene expression in rat
liver exposed to gold nanoparticles
VL - 12
ID - 8749
ER -
TY - JOUR
AB - Aquatic contamination with silver nanoparticles (Ag-NPs) can cause toxicity
in target organisms that are directly exposed to them. Oxidative stress and tissue
alterations are potential endpoints of Ag-NPs toxicity. We determined whether
garlic oil can ameliorate the effects of toxicity induced by amine-coated and
spherical Ag-NPs in 100 ± 5 g freshwater rohu Labeo rohita. The stock fish were
placed in tanks with continuous water supply. There were three treatments and a
control group, each with three replicates comprising five fish each. The treatments
were: garlic only, Ag-NPs only, and garlic plus Ag-NPs. The Ag-NPs (30 mg/L) was
supplied in water, whereas garlic oil (50 µl/kg) was given to the fish through a
prepared feed. The treatment duration was 28 days. In the control and garlic oil
treatment there were no observed alterations in fish. The Ag-NP treatment initiated
inflammation and necrosis in hepatic parenchyma, which resulted in venous
congestion. Garlic oil treatment reduced vascular congestion in hepatic tissues.
Ag-NPs also induced cellular vacuolization and the build-up of a yellow pigment.
Inflammatory gill filament cartilage and lamellae were observed, as were swelling
and collapse of lamellae, accumulation of macrophages, and necrosis and fusion of
secondary lamellae in gill sections of Ag-NP-treated fish. Garlic oil ameliorated
these changes, although some alterations like fused secondary lamellae, separation
between the epithelium and lamellae, and accumulation of macrophages were still
recorded in gill sections of garlic-treated fish. Garlic oil increased the activity
of antioxidants (superoxide dismutase, catalase, glutathione S-transferase and
glutathione) and caused a reduction in lipid peroxidation and/or MDA content.
Furthermore, garlic oil treatment also improved measured hematologic parameters.
This study confirms the role of garlic as a natural antioxidant for the
amelioration of oxidative stress and histological and hemotologic changes in fish
exposed to Ag-NPs. © 2020, Japanese Society of Fisheries Science.
AN - rayyan-553780728
AU - Khan, M. S.
AU - Qureshi, N. A.
AU - Jabeen, F.
AU - Wajid, M.
AU - Sabri, S.
AU - Shakir, M.
DO - 10.1007/s12562-020-01403-7
IS - 2
KW - Allium sativum
Colloidal silver
Free radical scavenging
Garlic oil
Nanoparticles
Nanopollution
Oxidative stress
Silver toxicity
Oxidative Stress
Garlic
PY - 2020
SP - 255-269
ST - The role of garlic oil in the amelioration of oxidative stress and tissue
damage in rohu Labeo rohita treated with silver nanoparticles
T2 - Fisheries Science
TI - The role of garlic oil in the amelioration of oxidative stress and tissue
damage in rohu Labeo rohita treated with silver nanoparticles
85080939614&doi=10.1007%2fs12562-020-01403-
7&partnerID=40&md5=0897e8b3d24c0182ca77bcb74e53d806
VL - 86
ID - 8750
ER -
TY - JOUR
AB - The development of multifunctional biomaterials for wound dressings,
bandages, tissue scaffolds, etc., has received massive attention in the biomedical
field in the last few years. The advanced progress in tissue engineering is the
fabrication of smart biomaterials mimicking extracellular matrix (ECM) of human
tissue, such as nanofibrous meshes with a micro and nanoporous structure. Silk
fibroin (SF) nanofiber is an ideal candidate as a vehicle/substitute material for
biomedical utilization due to its several unique properties, including high
biocompatibility, biodegradability, and deficient inflammatory reactions. These
characteristics make SF a favorable matrix for therapeutic agents. However,
unmodified SF could be oxidized quickly, exhibit hydrophobicity, making it
inappropriate for biological applications, and inclined to microbial attacks,
lessening its applicability. Intensive research is going on to extend the
capabilities of SF from hydrophobic material to hydrophilic, from the filament to
film, sheet, and scaffolds, and even from soft material to super-rigid material.
The researchers have traversed multiple approaches to remodel SF nanofibers to
improve the utilization of SF. The threat of infections is inevitable when using SF
as wound healing dressing elements or artificial grafts in tissue engineering
applications. To confer antimicrobial property to SF, the use of silver
nanoparticles and other silver composites is one of the strategies. Silver is a
natural antimicrobial agent and has been used since ancient times. Researchers have
incorporated silver in the SF nanofibers to impart antimicrobial properties and
improve their applications. This review describes the extraction process of SF from
silkworms using different methods and their comparative analysis. The
electrospinning of SF nanofibers and the important parameters that need to be
considered during the fabrication of electrospun SF nanofibers has also been
discussed. Furthermore, this review has focused on the current progress in
improvising SF nanofiber utilizing silver nanoparticles for antibacterial and wound
healing applications. © 2022 Elsevier Ltd
AN - rayyan-553780729
AU - Khan, R. S.
AU - Rather, A. H.
AU - Wani, T. U.
AU - Rather, S. U.
AU - Abdal-hay, A.
AU - Sheikh, F. A.
DO - 10.1016/j.mtcomm.2022.103914
KW - Electrospinning
Nanofiber
Silk fibroin
Tissue engineering
Wound healing
Biocompatibility
Biodegradability
Hydrophobicity
Metal nanoparticles
Microorganisms
Scaffolds (biology)
Tissue
Anti-microbial properties
Biomedical fields
Extracellular matrices
Smart biomaterials
Tissue scaffolds
Tissues engineerings
Wound dressings
Nanofibers
Wound Healing
PY - 2022
ST - A comparative review on silk fibroin nanofibers encasing the silver
nanoparticles as antimicrobial agents for wound healing applications
T2 - Materials Today Communications
TI - A comparative review on silk fibroin nanofibers encasing the silver
nanoparticles as antimicrobial agents for wound healing applications
85133933992&doi=10.1016%2fj.mtcomm.2022.103914&partnerID=40&md5=891537f9a5aac03c887
de155edb82016
VL - 32
ID - 8751
ER -
TY - JOUR
AB - Nanoparticles are emerging as a useful tool for a wide variety of biomedical,
consumer and instrumental applications that include drug delivery systems,
biosensors and environmental sensors. In particular, nanoparticles have been shown
to offer greater specificity with enhanced bioavailability and less detrimental
side effects as compared to the existing conventional therapies in nanomedicine.
Hence, bionanotechnology has been receiving immense attention in recent years.
However, despite the extensive use of nanoparticles today, there is still a limited
understanding of nanoparticle-mediated toxicity. Both in vivo and in vitro studies
have shown that nanoparticles are closely associated with toxicity by increasing
intracellular reactive oxygen species (ROS) levels and/or the levels of pro-
inflammatory mediators. The homeostatic redox state of the host becomes disrupted
upon ROS induction by nanoparticles. Nanoparticles are also known to up-regulate
the transcription of various pro-inflammatory genes, including tumor necrosis
factor-alpha and IL (interleukins)-1, IL-6 and IL-8, by activating nuclear factor-
kappa B (NF-kappa B) signaling. These sequential molecular and cellular events are
known to cause oxidative stress, followed by severe cellular genotoxicity and then
programmed cell death. However, the exact molecular mechanisms underlying
nanotoxicity are not fully understood. This lack of knowledge is a significant
impediment in the use of nanoparticles in vivo. In this review, we will provide an
assessment of signaling pathways that are involved in the nanoparticle-induced
oxidative stress and propose possible strategies to circumvent nanotoxicity.
AN - rayyan-553780730
AU - Khanna, P.
AU - Ong, C.
AU - Bay, B. H.
AU - Baeg, G. H.
DO - 10.3390/nano5031163
IS - 3
Inflammation
PY - 2015
SN - 2079-4991
SP - 1163-1180
ST - Nanotoxicity: An Interplay of Oxidative Stress, Inflammation and Cell Death
T2 - NANOMATERIALS
TI - Nanotoxicity: An Interplay of Oxidative Stress, Inflammation and Cell Death
VL - 5
Y2 - 9
ID - 8752
ER -
TY - JOUR
AB - Nanotechnology has vast applications in almost all fields of science and
technology. The use of medicinal plants for the synthesis of metallic nanoparticles
has gained much attention nowadays. In the current research work, six medicinal
plants were used for the synthesis of gold nanoparticles (AuNPs) and iron
nanoparticles (FeNPs). The synthesized nanoparticles were characterized by
different techniques including UV-visible spectrophotometry, scanning electron
microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Furthermore,
the activities of green synthesized nanoparticles were screened in vitro using, for
example, antibacterial, antioxidant, cytotoxic, and DNA protection assays. Both
FeNPs and AuNPs had spherical shapes with an average size less than 50 nm and were
found to have good antimicrobial and nontoxic effects. Furthermore, FeNPs from
Ficus microcarpa demonstrated high drug loading efficiency (65%) as compared to an
anti-inflammatory drug (diclofenac potassium, DFP). We also evaluated the drug
delivery potential, as well as anti-inflammatory and anticoagulant properties, of
nanoparticles in vivo. Interestingly, AuNPs of Syzygium cumini exhibited strong
anti-inflammatory potential as compared to DFP and diclofenac loaded FeNPs of Ficus
microcarpa. The results suggest potential pharmacological applications of biogenic
synthesized AuNPs and FeNPs which can be explored further. The study revealed that
the green synthesized AuNPs and FeNPs provide a promising approach for the
synthesis of drug-loaded nanoparticles and consequently in the field of targeted
drug delivery.
AN - rayyan-553780731
AU - Khanzada, B.
AU - Akthar, N.
AU - Bhatti, M. Z.
AU - Ismail, H.
AU - Alqarni, M.
AU - Mirza, B.
AU - Mostafa-Hedeab, G.
AU - Batiha, G. E. S.
DO - 10.1155/2021/1581444
KW - Oxalic Acid
Iron
Norisoprenoids
PY - 2021
SN - 2090-9063 2090-9071
ST - Green Synthesis of Gold and Iron Nanoparticles for Targeted Delivery: An In
Vitro and In Vivo Study
T2 - JOURNAL OF CHEMISTRY
TI - Green Synthesis of Gold and Iron Nanoparticles for Targeted Delivery: An In
Vitro and In Vivo Study
VL - 2021
Y2 - 11 y3 - 23
ID - 8753
ER -
TY - JOUR
AB - Introduction: Herbs are excellent sources of medicinal substances, and their
curative abilities have been recognized to treat many ailments and are used for
example as antioxidants, analgesics, anti-inflammatories, antipyretics, and many
other medicinal uses. The properties of natural compounds and their health effects
have been studied extensively, especially those that originate from plant sources
such as ginger. The ginger plant contains many chemical compounds, such as 6-
gingerol, which is characterized by containing active groups such as carbonyl and
hydroxide, which can be attached to metal molecules. This is what was done in this
study, where the formation of complexes with a group of metals was studied and
their effect on cancer cells was investigated. These complexes will open new
horizons for further study of medicinal uses. Methods: The synthesis of gingerol-
metal complexes was carried out by conjugating gingerol molecules with Ag, Au, Cd,
Co, Cu, Ni, and Zn metal ions. The extracted gingerol was transferred to culture
tubes and deionized water-DMSO were added followed by sonication. The tubes were
incubated at 90°C for two days as well as the control sample. The samples were then
filtered and the complex solutions were transferred into new tubes for further
studies. Different characterization techniques such as FT-IR, UVvis spectroscopy,
FESEM, and EDX are used to confirm the formation of the complexes. The in vitro of
the complexes was tested by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-
carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay against the
human colorectal cancer cell lines HCT116 and HT29 which exhibited strong
cytotoxicity. Results: The gingerol-metal complexes showed an enhancement as an
anticancer agent compared to the control. The in vitro anticancer activity showed
that the Ag-gingerol complex showed the most activity among the other complexes.
Discussion: Gingerol-metal complexes can inhibit cancer cells, noting that the
potency of the complex depends on the type of metal used. © 2023 Khdary et al.
AN - rayyan-553780732
AU - Khdary, N. H.
AU - Alangari, A. A.
AU - Katubi, K. M.
AU - Alanazi, M.
AU - Alhassan, A.
AU - Alzahrani, S. D.
AU - Khan, Z.
AU - Alanazi, I. O.
DO - 10.2147/CMAR.S391546
KW - Colon cancer
Ginger
Gingerol
Gingerol-complexes
Tumor cell line
analgesic agent
antioxidant
antipyretic agent
cadmium
carbonyl derivative
cobalt
copper
gingerol
gold
hydroxide
metal complex
natural product
nickel
silver
zinc
Article
cell viability
colon cancer cell line
controlled study
cytotoxicity
drug synthesis
elemental analysis
HCT 116 cell line
herb
HT-29 cell line
human
human cell
IC50
in vitro study
MTS assay
Humanities
Humanism
Humans
Colonic Neoplasms
Cell Line
PY - 2023
SP - 87-98
ST - Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human
Colon Cancer Cell Line
T2 - Cancer Management and Research
TI - Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human
Colon Cancer Cell Line
85148702475&doi=10.2147%2fCMAR.S391546&partnerID=40&md5=7f5ee89c182c394b3548ad1d169
ef269
VL - 15
ID - 8754
ER -
TY - JOUR
AB - Toxic chemicals are used in traditional nanoparticle synthesis processes,
resulting in environmental toxicity. As a result, we must switch to "green
synthesis." Previous studies have shown that Silver nanoparticles reinforced with
Amla extract have excellent antimicrobial properties. As a result, this research
was carried out to determine the anti-inflammatory and antioxidant properties of
Silver. Since Silver induces cell wall lysis due to intracellular material leakage,
it is an excellent antimicrobial agent and was chosen to make a nanoparticle. Aim:
Aim of the study was phyto assisted preparation of Silver nanoparticles from Amla
fruit and evaluation of its anti-inflammatory and antioxidant properties. Material
and methods: Anti-inflammatory and antioxidant properties of the nanoparticle were
assessed using Bovine Serum Albumin (BSA) and DPPH Assay respectively at 10 mu L,
20 mu L, 30 mu L, 40 mu L, 50 mu L. Results: Values for anti-inflammatory property
of nanoparticles were higher than the standard values at 40 mu L, 50 mu L
concentrations. Percentage of inhibition was highest at 40 mu L (86%) and 50 mu L
(84.6%). The values for antioxidant property of nanoparticles were found to be
higher than the standard values at concentrations except at 40 mu L, 50 mu L.
Percentage of inhibition was highest at 20 mu L (86.2%) Conclusion: Within the
limits of the study it can be concluded that Silver nanoparticles have exceptional
antiinflammatory and antioxidant properties and further can be incorporated in
dental material or can be used to coat suture materials to improve their
properties.
AN - rayyan-553780733
AU - Khullar, D.
AU - Ahmed, N.
IS - 8
KW - Antioxidants
PY - 2022
SN - 2347-2545 2347-2367
SP - 47-+
ST - Green Synthesis, Characterization and Assessment of Anti-inflammatory and
Antioxidant Properties of Silver Nanoparticles Prepared using Amla Extracts-An In-
vitro Study
T2 - JOURNAL OF RESEARCH IN MEDICAL AND DENTAL SCIENCE
TI - Green Synthesis, Characterization and Assessment of Anti-inflammatory and
Antioxidant Properties of Silver Nanoparticles Prepared using Amla Extracts-An In-
vitro Study
VL - 10
ID - 8755
ER -
TY - JOUR
AB - Hemorrhage in mice results in decreased ATP levels in the jejunum, lung,
kidney, heart, and brain but not in liver tissue lysates, albeit at variable levels
and time kinetics. The decreased protein expression and activity of pyruvate
dehydrogenase ( PDH) accounted for the hemorrhage-induced ATP loss. Treatment with
geldanamycin (GA; 1 mu g/g body wt), a known inducer of heat shock protein (HSP)70,
inhibited the hemorrhage-induced ATP loss in the jejunum, lung, heart, kidney, and
brain. GA was found to increase PDH protein, preserve PDH enzymatic activity, and
inhibit mucosal injury in jejunum tissues. GA-induced HSP70i was found to form
complexes with PDH protein. HSP70 gene transfer into intestinal epithelial cells
promoted PDH and ATP levels, whereas HSP70 short interfering RNA limited them. We
conclude that agents able to increase the expression of HSP70 and PDH may be of
value in reducing pathology resulting from hemorrhage-associated ATP loss.
AN - rayyan-553780734
AU - Kiang, J. G.
AU - Bowman, P. D.
AU - Lu, X. Y.
AU - Li, Y. S.
AU - Ding, X. Z.
AU - Zhao, B. T.
AU - Juang, Y. T.
AU - Atkins, J. L.
AU - Tsokos, G. C.
DO - 10.1152/ajpgi.00397.2005
IS - 1
KW - Pyruvic Acid
Pyruvates
PY - 2006
SN - 0193-1857 1522-1547
SP - G117-G127
ST - Geldanamycin prevents hemorrhage-induced ATP loss by overexpressing inducible
HSP70 and activating pyruvate dehydrogenase
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
TI - Geldanamycin prevents hemorrhage-induced ATP loss by overexpressing inducible
HSP70 and activating pyruvate dehydrogenase
VL - 291
Y2 - 7
ID - 8756
ER -
TY - JOUR
AB - Soft tissue trauma and hemorrhage (T-H) diminishes various aspects of liver
function, while it increases hepatic nitrate/nitrite, inducible nitric oxide
synthase (iNOS), and endothelin-1 levels. Treatment with androstenediol (AED)
inhibits the T-H-induced alterations of the above parameters. We sought to identify
the molecular events underlying the beneficial effect of AED. Exposure of rats to
T-H significantly increased the caspase-3 activity and protein, whereas treatment
with AED significantly limited these increases. AED treatment also suppressed the
T-H-induced increase in iNOS by effectively altering the levels of key
transcription factors involved in the regulation of iNOS expression.
Immunoprecipitation and immunoblotting analyses indicate that T-H increased
apoptosome formation, and AED treatment significantly decreased it. Modulating the
iNOS protein by transfecting cells with iNOS gene or small interfering RNA further
confirmed the correlation between iNOS and caspase-3. Our data indicate that AED
limits caspase-3 expression by suppressing the expression of transcription factors
involved in the production of iNOS, resulting in decreased apoptosome. AED can
potentially be a useful adjuvant for limiting liver apoptosis following T-H shock.
AN - rayyan-553780735
AU - Kiang, J. G.
AU - Peckham, R. M.
AU - Duke, L. E.
AU - Shimizu, T.
AU - Chaudry, I. H.
AU - Tsokos, G. C.
DO - 10.1152/japplphysiol.00919.2006
IS - 3
KW - Nitric Oxide Synthase
Nitric Oxide Synthase Type II
Caspases
Androstenediol
Androstenediols
PY - 2007
SN - 8750-7587 1522-1601
SP - 933-941
ST - Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3
by downregulating the inducible nitric oxide synthase pathway
T2 - JOURNAL OF APPLIED PHYSIOLOGY
TI - Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3
by downregulating the inducible nitric oxide synthase pathway
VL - 102
Y2 - 3
ID - 8757
ER -
TY - JOUR
AB - Today nanosciences are experiencing massive investment worldwide although
research on toxicological aspect of these nano-sized particles has just begun and
to date, no clear guidelines exist to quantify the effects. In the present study,
we focus on silver nanoparticles, which represent one of the most widely
investigated nanoparticles. The present data indicate that silver nanoparticles
seem to cross the cellular membrane of various tissues in Sprague Dawley rat and,
therefore, might have an influence on cell physiology and function. Rats are
exposed via oral administration and intravenous injection with commercial silver
nanoparticles. Three types of silver nanoparticels are used in this study: 1) Type
I, particle size 50-90 nm with no dispersant, 2) Type II, particle size 1-10 nm
dispersed with several amino acids. After 4 weeks exposure we examined the clinical
indicators from blood and also analyzed histological changes in various tissues,
including liver, kidney, and lung, to investigate the histopathological changes.
The concentration of 2 indicators, total cholesterol and creatinine were changed
with statistical importance. Also, lymphocytes/granulocyte ratio was significantly
increased by silver nanoparticles. The histological change had accordance with the
change of clinical indicators. The inflammatory symptoms were observed in liver
tissue and it lead to the result that the hematologic/lymphocytic disorder, not
hepatic disorder, would be related to silver nanoparticle toxicity. Finally we
propose 4 genes as size independent genomic biomarkers and 10 genes as
representative biomarkers for histopathological and clinical changes for silver
nanoparticle exposure. © 2009 The Korean Society of Environmental Risk Assessment
and Health Science and Springer.
AN - rayyan-553780739
AU - Kim, E.
AU - Maeng, J. H.
AU - Lee, D. H.
AU - Kim, J. M.
DO - 10.1007/BF03216458
IS - 1
KW - Biomarker
Silver nanoparticle
Toxicogenomics
Biological Markers
PY - 2009
SP - 8-16
ST - Correlation of biomarkers and histological responses in manufactured silver
nanoparticle toxicity
T2 - Toxicology and Environmental Health Sciences
TI - Correlation of biomarkers and histological responses in manufactured silver
nanoparticle toxicity
84873250628&doi=10.1007%2fBF03216458&partnerID=40&md5=d13fb4cbbaa5d27b99aebe5f400e1
918
VL - 1
ID - 8761
ER -
TY - JOUR
AB - Mast cells are responsible for IgE-mediated allergic responses through the
secretion of various inflammatory cytokines and mediators. Therefore, the
pharmacological regulation of mast cell activation is an important goal in the
development of novel anti-allergic drugs. In this study, we found that spiraeoside
(SP) inhibits mast cell activation and allergic responses in vivo. SP dose-
dependently inhibited the degranulation induced by IgE-antigen (Ag) stimulation in
RBL-2H3 mast cells without cytotoxic effects. At the molecular level, SP reduced
the Ag-induced phosphorylation and subsequent activation of phospholipase C-γ2
(PLC-γ2). Moreover, SP inhibited the phosphorylation of spleen tyrosine kinase
(Syk), linker for activation of T cells (LAT), and downstream MAPKs, such as
ERK1/2, p38, and JNK, eventually attenuating expression of TNF-κ and IL-4. Finally,
we found that SP significantly inhibited IgE-mediated passive cutaneous anaphylaxis
(PCA) in mice. Taken together, our results strongly suggest that SP suppresses IgE-
mediated mast cell activation and allergic responses by inhibiting Lyn-induced PLC-
γ2/MAPK signaling in mast cells.
AN - rayyan-553780742
AU - Kim, J. K.
AU - Seo, Y. K.
AU - Park, S.
AU - Park, S. A.
AU - Lim, S.
AU - Lee, S.
AU - Kwon, O.
AU - Seo, J. K.
AU - Choi, U. K.
AU - Ryu, S. H.
AU - Suh, P. G.
DO - 10.1139/bcb-2014-0055
IS - 3
KW - allergic response
degranulation
Lyn/Syk kinase
MAPK
mast cells
PLC-γ
Spiraeoside
Animals
Cell Line
Cytokines
Immunoglobulin E
Male
Mast Cells
Mice, Inbred BALB C
Passive Cutaneous Anaphylaxis
Phospholipase C gamma
Phosphorylation
Quercetin
Rats
Signal Transduction
src-Family Kinases
Mus
Amino acids
Cells
Chemical activation
Cytology
Cytotoxicity
Enzymes
Silver
calcium ion
immunoglobulin E
Janus kinase
LAT protein
mitogen activated protein kinase
phospholipase C gamma
protein kinase Syk
RNA
silver
spiraeoside
cytokine
protein tyrosine kinase
quercetin
Allergic response
Degranulation
Mast cells
allergic reaction
allergy
animal cell
animal experiment
Article
cell viability
drug effect
enzyme activation
leukemia cell line
mast cell
mast cell leukemia
mouse
nonhuman
passive skin anaphylaxis
rat
Western blotting
analogs and derivatives
animal
Bagg albino mouse
cell line
drug effects
immunology
male
metabolism
phosphorylation
signal transduction
Cell signaling
Type C Phospholipases
Phospholipases
PY - 2015
SP - 227-235
ST - Spiraeoside inhibits mast cells activation and IgE-mediated allergic
responses by suppressing phospholipase C-γ-mediated signaling
T2 - Biochemistry and Cell Biology
TI - Spiraeoside inhibits mast cells activation and IgE-mediated allergic
responses by suppressing phospholipase C-γ-mediated signaling
84946240688&doi=10.1139%2fbcb-2014-
0055&partnerID=40&md5=91a144efccb17ff7a20e5c70d86dbcc6
VL - 93
ID - 8764
ER -
TY - JOUR
AB - Resistance to tumor necrosis factor-related apoptosis-inducing ligand
(TRAIL)-induced apoptosis has been reported in some cancer cells, including AGS
human gastric adenocarcinoma cells. Hizikia fusiforme is a commonly used brown
seaweed species in Korea that possesses potent antibacterial, antifungal, and anti-
inflammatory activities. In this study, we demonstrated that treatment with TRAIL
in combination with subtoxic concentrations of ethyl alcohol extract of H.
fusiforme (EAHF) sensitized TRAIL-resistant AGS cells to TRAIL-mediated apoptosis.
Combined treatment with EAHF and TRAIL increased chromatin condensation, DNA
fragmentation, and sub-G1-phase DNA content. The restored sensitivity to TRAIL-
induced apoptosis appeared to be correlated with the modulation of Bcl-2 family
proteins and activation of caspases, which resulted in the cleavage of poly(ADP-
ribose) polymerase. Taken together, the use of EAHF in combination with TRAIL may
be an effective and selective anticancer strategy via suppressing the resistance to
TRAIL-induced apoptosis in some tumor cell lines, including AGS cells.
AN - rayyan-553780746
AU - Kim, T. Y.
AU - Jin, C. Y.
AU - Kim, G. Y.
AU - Choi, I. W.
AU - Jeong, Y. K.
AU - Nam, T. J.
AU - Kim, S. K.
AU - Choi, Y. H.
DO - 10.1089/jmf.2008.1114
IS - 4
KW - Humanities
Humanism
Humans
Ethanol
Apoptosis
Adenocarcinoma
PY - 2009
SN - 1096-620X 1557-7600
SP - 782-787
ST - Ethyl Alcohol Extracts of Hizikia fusiforme Sensitize AGS Human Gastric
Adenocarcinoma Cells to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-
Mediated Apoptosis
T2 - JOURNAL OF MEDICINAL FOOD
TI - Ethyl Alcohol Extracts of Hizikia fusiforme Sensitize AGS Human Gastric
Adenocarcinoma Cells to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-
Mediated Apoptosis
VL - 12
Y2 - 8
ID - 8768
ER -
TY - JOUR
AB - Nano-silver (Ag) with antimicrobial activity is by far the most
commercialized nano-compound. The hazards associated with human exposure to
nanosized-silver should be investigated to facilitate the risk assessment process.
Recent studies have shown that inflammatory, oxidative, genotoxic, and cytotoxic
consequences are associated with silver particulate exposure, and are inherently
linked. In the present study, the cytotoxicity and genotoxicity of nano-silver were
investigated using the dye exclusion assay, the comet assay, and the mouse lymphoma
thymidine kinase (tk+/-) gene mutation assay (MLA). IC20 values of nano-silver in
L5178Y cells were determined the concentration of 3,769.53 μg/mL and 1,796.88 μg/mL
with and without S-9, respectively. And in BEAS-2B cell, IC20 values were
calculated to 1,171.88 μg/mL and 761.72 μg/mL with and without S-9, respectively.
From these results, nano-silver was more cytotoxic in absence of S-9 metabolic
activation system and at the BEAS-2B cells. In the comet assay, L5178Y cells and
BEAS-2B cells were treated with nano-silver which significantly increased >2-fold
tail moment with and without S-9. However, the mutant frequencies in the nano-
silver treated L5178Y cells were slightly increased but not significant compared to
the vehicle controls with and without S-9. The results of this study indicate that
nano-silver can cause primary DNA damage and cytotoxicity but not mutagenicity in
cultured mammalian cells. © 2010 The Korean Society of Toxicogenomics and
Toxicoproteomics and Springer Netherlands.
AN - rayyan-553780747
AU - Kim, Y. J.
AU - Yang, S. I.
AU - Ryu, J. C.
DO - 10.1007/s13273-010-0018-1
IS - 2
KW - BEAS-2B cell
Comet assay
Cytotoxicity
Gene mutation assay (MLA)
L5178Y cell
Nano-silver
Cell Line
PY - 2010
SP - 119-125
ST - Cytotoxicity and genotoxicity of nano-silver in mammalian cell lines
T2 - Molecular and Cellular Toxicology
TI - Cytotoxicity and genotoxicity of nano-silver in mammalian cell lines
77956565849&doi=10.1007%2fs13273-010-0018-
1&partnerID=40&md5=610ed7548509a52a3952125eef03c1bf
VL - 6
ID - 8769
ER -
TY - JOUR
AB - In the present study, aqueous leaf extract of Strobilanthes cordifolia
J.R.I.Wood was combined with silver nitrate to synthesis silver nanoparticles
(AgNPs). The AgNPs were characterized using visible spectroscopy (UV), X-ray
diffraction (XRD), Fourier transform infrared spectrophotometer (FTIR), scanning
electron microscope (SEM), energy dispersive X-ray (EDaX), particle size analysis,
and transmission electron microscope (TEM). The UV spectrum absorption peak
occurred at 438 nm. The FTIR analysis of the AgNPs indicated the presence of
functional groups such as aldehyde, alkenes, and carboxylic acids. The crystalline
structure of AgNPs was confirmed by XRD. The AgNPs have a spherical shape according
to SEM. The AgNPs components composition was confirmed by EDaX. The particle size
distribution of AgNPs is monodispersion in the range at 42.54 nm. TEM demonstrated
the AgNPs size to be between 11.35 and 34.90 nm. The AgNPs exhibited good
antibacterial property against Escherichia coli and Staphylococcus aureus. The
antioxidant activity of the AgNPs was represented by increased DPPH, ABTS, and H2O2
activities.The antidiabetic activity of the AgNPs was indicated by the inhibition
of α-amylase and α-glycosidase and anti-inflammatory highest albumin denaturation
and HRBC membrane stabilization properties. Further, the AgNPs also significantly
inhibited the MCF-7 cell lines. These results clearly suggest that the synthesized
AgNPs using S. cordifolia leaves could have several potential biomedical
applications. © 2022 International Union of Biochemistry and Molecular Biology,
Inc.
AN - rayyan-553780748
AU - Kirubakaran, D.
AU - Selvam, K.
AU - Prakash, P.
AU - Manimegalai, P.
AU - Shivakumar, M. S.
AU - SenthilNathan, S.
DO - 10.1002/bab.2406
IS - 2
KW - anti-inflammatory
antibacterial
antidiabetic
antioxidant
antiproliferative
characterization
green synthesis
Metal Nanoparticles
Plant Extracts
Silver
Wood
X-Ray Diffraction
Absorption spectroscopy
Cell culture
Escherichia coli
Metal nanoparticles
Particle size
Silver compounds
Ultraviolet spectroscopy
X ray diffraction
albumin
aldehyde
alkene
alpha glycosidase
amylase
ascorbic acid
carboxylic acid
glycosidase
intestine enzyme
silver nanoparticle
silver nitrate
unclassified drug
antiinfective agent
metal nanoparticle
plant extract
silver
Anti-inflammatories
Anti-proliferative
Antibacterials
Antidiabetic
Characterization
Fourier transform infrared spectrophotometers
Green synthesis
Scanning electrons
Visible spectroscopy
X- ray diffractions
Acanthaceae
Article
biological activity
biosynthesis
chemical analysis
controlled study
crystal structure
cytotoxicity assay
denaturation
drug absorption
drug synthesis
human
human cell
hydrogen peroxide scavenging assay
MCF-7 cell line
membrane stabilization
nanotechnology
optical spectroscopy
particle size
plant leaf
Strobilanthes cordifolia
wood
zeta potential
chemistry
Antioxidants
PY - 2023
SP - 870-884
ST - Preparation and characterization of biogenic silver nanoparticles using
Strobilanthes cordifolia (Vahl) J.R.I. Wood leaves and its biological applications
T2 - Biotechnology and Applied Biochemistry
TI - Preparation and characterization of biogenic silver nanoparticles using
Strobilanthes cordifolia (Vahl) J.R.I. Wood leaves and its biological applications
85138739928&doi=10.1002%2fbab.2406&partnerID=40&md5=9bb28a3022caf3efd884d789ec3a37f
8
VL - 70
ID - 8770
ER -
TY - JOUR
AB - Silver oxide nanoparticles (AgO-NPs) antioxidant, anti-cancer, anti-
microbial, and tissue repair properties. Gouty arthritis is the inflammation of
tissues and joints caused by the deposition of monosodium urate crystals. In this
experiment, we investigated the anti-hyperuricemic effectiveness of different
concentrations of AgO-NPs in mice. The present study aimed to investigate the
effect of administration of AgO-NPs in monosodium urate (MSU)-induced gouty mice
for the very first time. Monosodium urate (MSU) crystals were administered
intraperitoneal for gout induction, followed by 5, 10, and 20 mu g/mL doses of AgO-
NPs for 2 weeks. The positive control was provided with the commercially available
drug allopurinol to compare the effects of AgO-NPs and allopurinol. The main
purpose of the study was to investigate the effectiveness of the nanoparticles in
comparison with commercially available drugs. AgO-NPs have been shown to improve
the condition of gouty arthritis by reducing significantly (P < 0.001) increased
levels of ALT, AST, and total bilirubin. The total protein estimation results
showed significant improvement at concentration of 20 mu g/mL of AgO-NPs. The lipid
profile results showed that high concentration (20 mu g/mL) of AgO-NPs decrease the
lipid content significantly as compared to control. It was concluded from this
study that the antioxidant, anti-inflammatory, and antilipidemic properties of AgO-
NPs may improve the hyperuricemic condition in gouty arthritis mice.
AN - rayyan-553780749
AU - Kiyani, M. M.
AU - Moghul, N. B.
AU - Javed, A.
AU - Butt, M. A.
AU - Abbas, H. B.
AU - Rehman, H.
AU - Rajput, T. A.
AU - Bokhari, S. A. I.
DO - 10.1007/s12011-021-02960-3
IS - 8
KW - Mice
PY - 2022
SN - 0163-4984 1559-0720
SP - 3677-3687
ST - In Vivo Effects of Orally Administered Different Concentrations of Silver
Oxide Nanoparticles in Hyperuricemic Mice
TI - In Vivo Effects of Orally Administered Different Concentrations of Silver
Oxide Nanoparticles in Hyperuricemic Mice
VL - 200
Y2 - 8
ID - 8771
ER -
TY - JOUR
AB - The number of patients suffering from chronic wound healing disorders in
Germany alone is estimated to be 2.5-4 million. Therapy related expenses reach 5-8
billion Euros annually. This number is partially caused by costly dressing changes
due to non-standardized approaches and the application of non-evidence-based
topical wound therapies. The purpose of this paper is to elucidate a
straightforward principle for the management of chronic wounds, and to review the
available evidence for the particular therapy options. The T.I.M.E.-principle
(Tissue management, Inflammation and infection control, Moisture balance,
Epithelial [edge] advancement) was chosen as a systematic strategy for wound bed
preparation. Literature was retrieved from the PubMed and Cochrane Library
databases and subjected to selective analysis. Topical wound management should be
carried out according to a standardized principle and should further be
synchronized to the phases of wound healing. Despite the broad implementation of
these products in clinical practice, often no benefit exists in the rate of
healing, when evaluated in meta-analyses or systematic reviews. This insufficient
evidence is additionally limited by varying study designs. In case of non-
superiority, the results suggest to prefer relatively inexpensive wound dressings
over expensive alternatives. Arbitrary endpoints to prove the effectiveness of
wound dressings, contribute to the random use of such therapies. Defining rational
endpoints for future studies as well as the deployment of structured therapy
strategies will be essential for the economical and evidence-based management of
chronic wounds. © The Authors | Journal compilation © Blackwell Verlag GmbH,
Berlin.
AN - rayyan-553780750
AU - Klein, S.
AU - Schreml, S.
AU - Dolderer, J.
AU - Gehmert, S.
AU - Niederbichler, A.
AU - Landthaler, M.
AU - Prantl, L.
DO - 10.1111/ddg.12138
IS - 9
KW - Administration, Topical
Bandages
Combined Modality Therapy
Debridement
Evidence-Based Medicine
Humans
Skin
Soft Tissue Injuries
Wound Healing
alginic acid
antiinfective agent
cadexomer iodine
calcium alginate
carboxymethylcellulose
elase
iodine
iruxol
iruxolum mono
natural product
octenidine
polyhexanide
povidone iodine
silver
streptodornase plus streptokinase
unclassified drug
angiogenesis
antisepsis
artificial skin
autolytic debridement
bacterial colonization
bacterial infection
biocompatibility
chronic inflammation
chronic vein insufficiency
chronic wound
clinical effectiveness
clinical practice
Cochrane Library
colony forming unit
compression bandage
compression therapy
debridement
decubitus
Diptera
drug efficacy
drug induced disease
epithelization
evidence based medicine
fibroblast
foam dressing
granulation tissue
hemosiderosis
honey
human
hydrocolloid
hydrogel
hypoxemia
immunopathology
infection control
infection risk
ionizing radiation
keratinocyte
lavage
lipodermatosclerosis
Lucilia sericata
maggot therapy
malignant transformation
Manuka honey
marjolin ulcer
mechanical debridement
medical literature
Medline
meta analysis (topic)
metabolic disorder
moisture
neoplasm
pathophysiology
peripheral occlusive artery disease
plastic surgery
purpura
review
skin epithelium
skin ulcer
systematic review (topic)
tissue injury
tissue pressure
topical treatment
wound care
wound dressing
wound healing
wound infection
PY - 2013
SP - 819-829
ST - Evidence-based topical management of chronic wounds according to the T.I.M.E.
principle
T2 - JDDG - Journal of the German Society of Dermatology
TI - Evidence-based topical management of chronic wounds according to the T.I.M.E.
principle
84883487301&doi=10.1111%2fddg.12138&partnerID=40&md5=b5c17bcedeff9d71714319737c6c50
16
VL - 11
ID - 8772
ER -
TY - JOUR
AN - rayyan-553782421
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AU - Yu, H.
AU - Yu, J. J.
AU - Yu, J. T.
AU - Yu, J.
AU - Yu, L.
AU - Yu, W. H.
AU - Yu, X. F.
AU - Yu, Z.
AU - Yuan, J.
AU - Yuan, Z. M.
AU - Yue, B. Y.
AU - Yue, J.
AU - Yue, Z.
AU - Zacks, D. N.
AU - Zacksenhaus, E.
AU - Zaffaroni, N.
AU - Zaglia, T.
AU - Zakeri, Z.
AU - Zecchini, V.
AU - Zeng, J.
AU - Zeng, M.
AU - Zeng, Q.
AU - Zervos, A. S.
AU - Zhang, D. D.
AU - Zhang, F.
AU - Zhang, G.
AU - Zhang, G. C.
AU - Zhang, H.
AU - Zhang, J.
AU - Zhang, J. P.
AU - Zhang, L.
AU - Zhang, M. Y.
AU - Zhang, X.
AU - Zhang, X. D.
AU - Zhang, Y.
AU - Zhao, M.
AU - Zhao, W. L.
AU - Zhao, X.
AU - Zhao, Y. G.
AU - Zhao, Y.
AU - Zhao, Y. X.
AU - Zhao, Z.
AU - Zhao, Z. J.
AU - Zheng, D.
AU - Zheng, X. L.
AU - Zheng, X.
AU - Zhivotovsky, B.
AU - Zhong, Q.
AU - Zhou, G. Z.
AU - Zhou, G.
AU - Zhou, H.
AU - Zhou, S. F.
AU - Zhou, X. J.
AU - Zhu, H.
AU - Zhu, W. G.
AU - Zhu, W.
AU - Zhu, X. F.
AU - Zhu, Y.
AU - Zhuang, S. M.
AU - Zhuang, X.
AU - Ziparo, E.
AU - Zois, C. E.
AU - Zoladek, T.
AU - Zong, W. X.
AU - Zorzano, A.
AU - Zughaier, S. M.
DO - 10.1080/15548627.2015.1100356
IS - 1
J2 - Autophagy
KW - Animals
*Autophagy/physiology
Biological Assay/methods/*standards
Computer Simulation
Humans
LA - eng
N1 - boc University of Michigan , Department of Molecular , Cellular, and
Developmental Biology , Ann Arbor , MI , USA.; bog University of Michigan, Life
Sciences Institute , Ann Arbor , MI , USA.; afh National Institute on Aging,
National Institutes of Health, Biomedical Research Center, RNA Regulation Section,
Laboratory of Genetics , Baltimore , MD , USA.; auo Tokyo Medical University ,
Department of Biochemistry , Tokyo , Japan.; bot University of Minnesota ,
Department of Lab Medicine and Pathology , Minneapolis , MN , USA.; pi Hebrew
University of Jerusalem, Faculty of Agriculture, Food, and Environment,
Biochemistry and Food Science , Rehovot , Israel.; adi MRC Harwell, Mammalian
Genetics Unit , Oxfordshire , UK.; bqf University of Occupational and Environmental
Health School of Medicine , Department of Neurology , Fukuoka , Japan.; ble
University of Iowa , Department of Internal Medicine , Iowa City , IA , USA.; be
Beatson Institute for Cancer Research, University of Glasgow , Glasgow , UK.; bxe
University of Toronto, Molecular Structure and Function, Research Institute,
Hospital for Sick Children , Toronto, ON , Canada.; bjm University of Florida ,
Department of Applied Physiology and Kinesiology , Gainesville , FL , USA.; zx Los
Angeles Biomedical Research Institute at Harbor-UCLA Medical Center , Torrance ,
CA.; bo Ben-Gurion University of the Negev and Mental Health Center , Department of
Clinical Biochemistry and Pharmacology and Psychiatry Research Unit , Beer-Sheva ,
Israel.; bia University of Colorado Denver, Skaggs School of Pharmacy and
Pharmaceutical Sciences , Department of Pharmaceutical Sciences , Aurora , CO ,
USA.; bgc University of California San Francisco , Department of Neurological
Surgery , San Francisco , CA , USA.; brf University of Palermo , Dipartimento di
Scienze e Tecnologie Biologiche , Chimiche e Farmaceutiche (STEBICEF) , Palermo ,
Italy.; yn KU Leuven, Laboratory for Cell Death Research and Therapy , Department
of Cellular and Molecular Medicine , Campus Gasthuisberg , Leuven , Belgium.; bwj
University of Texas, Medical Branch , Department of Pathology , Galveston , TX ,
USA.; add Icahn School of Medicine at Mount Sinai , Department of Otolaryngology ,
Tisch Cancer Institute at Mount Sinai , New York , NY , USA.; adf Icahn School of
Medicine at Mount Sinai, Division of Hematology and Oncology , Department of
Medicine , New York , NY , USA.; ck Broad Institute of MIT and Harvard ,
Cambridge , MA , USA.; pf Harvard University , Department of Statistics , Cambridge
, MA , USA.; azx Université Paris Diderot, Sorbonne Paris Cité, Centre Epigénétique
et Destin Cellulaire, UMR 7216, Centre National de la Recherche Scientifique CNRS ,
Paris , France.; byh University of Vienna , Department of Chromosome Biology , Max
F. Perutz Laboratories , Vienna , Austria.; ape Sidra Medical and Research Centre ,
Doha , Qatar.; bap University College Dublin, School of Chemical and Bioprocess
Engineering , Dublin , Ireland.; awt Universidad de Oviedo , Departamento de
Biología Funcional , Oviedo , Spain.; my Georgetown University, Lombardi
Comprehensive Cancer Center , Departments of Oncology and Pathology , Washington,
DC , USA.; uo IRCCS-Istituto di Ricerche Farmacologiche Mario Negri , Department of
Neuroscience , Milan , Italy.; sw Institut Pasteur , Department of Immunology ,
Paris , France.; dx Centro de Biologia Molecular "Severo Ochoa" (UAM/CSIC), Centro
de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas
(CIBERNED) , Madrid , Spain.; art Technische Universität München, II. Medizinische
Klinik, Klinikum rechts der Isar , Munich , Germany.; atb The Scripps Research
Institute , Department of Immunology and Microbial Science , La Jolla , CA , USA.;
xr KERBASQUE, Basque Foundation for Sciences , Bilbao , Spain.; ahd Neurogenomiks ,
Neurosciences Department , Faculty of Medicine and Odontology, University of Basque
, Leioa , Spain.; gx Cleveland Clinic , Department of Cancer Biology , Cleveland ,
OH , USA.; td Institute of Biomedical Investigation (INIBIC), Aging, Inflamation
and Regenerative Medicine , Coruña , Spain.; atp Thomas Jefferson University ,
Department of Biochemistry and Molecular Biology , Philadelphia , PA , USA.; tx
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) ,
Departamento de Biotecnología , Madrid , Spain.; afp National Institutes of Health,
National Heart, Lung, and Blood Institute , Bethesda , MD , USA.; ati The Wistar
Institute , Philadelphia , PA , USA.; beo University of Buenos Aires, Institute of
Biochemistry and Biophysics, School of Pharmacy and Biochemistry , Buenos Aires ,
Argentina.; baq University College London , Department of Clinical Neurosciences ,
London , UK.; dc CEA/DSV/12;BM, INSERM U1169, Gene Therapy for Neurodegenerative
Diseases , Fontenay-aux-Roses Cedex , France.; agb National Research Council (CNR),
Institute of Translational Pharmacology (IFT) , Rome , Italy.; ait Osaka University
Graduate School of Dentistry , Department of Preventive Dentistry , Osaka , Japan.;
bgt University of Camerino, School of Biosciences and Veterinary Medicine ,
Camerino , Italy.; bdb University of Barcelona, School of Medicine, Campus
Bellvitge , Hospitalet del Llobregat , Spain.; acb Medical Research Council (MRC),
Toxicology Unit , Leicester , UK.; aih Ohio State University , Department of
Microbial Infection and Immunity , Columbus , OH , USA.; cbi Wayne State
University, School of Medicine , Department of Pathology , Karmanos Cancer
Institute , Detroit , MI , USA.; aax Massachusetts Institute of Technology, Koch
Institute for Integrative Cancer Research , Cambridge , MA , USA.; bgd University
of California San Francisco , Department of Neurology , San Francisco , CA , USA.;
ke Emory University, School of Medicine, Division of Digestive Diseases , Atlanta ,
GA , USA.; f Aarhus University , Department of Molecular Biology and Genetics ,
Aarhus , Denmark.; cay Washington University in St. Louis, School of Medicine ,
Department of Ophthalmology and Visual Sciences , St. Louis , MO , USA.; blz
University of Leicester , Department of Cancer Studies , Leicester , UK.; sh INSERM
UMR1037, Centre de Recherches en Cancérologie de Toulouse , Toulouse , France.; byy
University of Zaragoza , Department of Biochemistry and Molecular and Cell
Biology , Faculty of Sciences , Zaragoza , Spain.; bf Beckman Research Institute,
City of Hope , Department of Molecular Pharmacology , Duarte , CA , USA.; nx
Griffith University, Menzies Health Institute Queensland , Australia.; aez National
Institute for Infectious Diseases "L. Spallanzani" IRCCS , Rome , Italy.; afz
Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Germany.;
bpw University of Niigata , Department of Neurosurgery , Brain Research Institute ,
Niigata , Japan.; bye University of Valencia , Department of Pharmacology ,
Valencia , Spain.; ber University of Calabria , Department of Pharmacy , Health and
Nutritional Sciences , Arcavacata di Rende (Cosenza) , Italy.; btd University of
Rome "Tor Vergata" , Department of Biology , Rome , Italy.; kf Emory University,
School of Medicine , Emory Vaccine Center and Department of Microbiology and
Immunology , Atlanta , GA , USA.; cbr Weizmann Institute of Science , Department of
Molecular Genetics , Rehovot , Israel.; qn IATA-CSIC, Institute of Agrochemistry
and Food Technology , Paterna (Valencia) , Spain.; vs Jikei University School of
Medicine, Divison of Respiratory Disease , Department of Internal Medicine ,
Tokyo , Japan.; bdp University of Bern, Division of Pediatric Hematology/Oncology ,
Department of Clinical Research , Bern , Switzerland.; z Albert Einstein College of
Medicine , Department of Developmental and Molecular Biology , Institute for Aging
Studies , Bronx , NY , USA.; auc Tohoku University, Graduate School of Life
Sciences, Sendai , Miyagi , Japan.; bog University of Michigan, Life Sciences
Institute , Ann Arbor , MI , USA.; bvf University of Sunderland , Department of
Pharmacy , Health and Wellbeing, Faculty of Applied Sciences , Sunderland , UK.;
bpe University of Namur, Laboratory of Biochemistry and Cell iology (URBC), Namur
Research Institute for Life Sciences (NARILIS) , Namur , Belgium.; ccn York
College/The City University of New York , Department of Biology , Jamaica , NY ,
USA.; yy Kyoto University, Graduate School of Medicine, Medical Innocation Center
(TMK project) , Kyoto , Japan.; buz University of Southern California, Keck School
of Medicine, Neurology and Pathology , Los Angeles , CA , USA.; bsu University of
Quebec at Trois-Rivieres , Department of Biology and Medicine , Trois-Rivieres,
Quebec , Canada.; adn Nagasaki University Graduate School of Biomedical Sciences ,
Department of Molecular Microbiology and Immunology , Nagasaki , Japan.; ne Georgia
Regents University, Medical College of Georgia , Augusta , GA , USA.; aak Macquarie
University , Department of Biomedical Sciences , Faculty of Medicine and Health
Sciences , Sydney , NSW , Australia.; aqm Stanford University, School of Medicine,
Departments of Radiation Oncology and Genetics , Stanford , CA , USA.; bps
University of Nice, INSERM U1065, C3M , Nice , France.; no Goethe University
Medical School, Experimental Neurology , Frankfurt am Main , Germany.; bnk
University of Maryland, School of Medicine , Department of Pharmacology , Baltimore
, MD , USA.; bxp University of Turin , Department of Clinical and Biological
Sciences , Turin , TO , Italy.; amo San Paolo Hospital Medical School, Unit of
Obstetrics and Gynecology , Milano , Italy.; bom University of Milan , Department
of Health Sciences , Milan , Italy.; mz Georgetown University, Lombardi
Comprehensive Cancer Center , Washington, DC , USA.; arv Tel Aviv University ,
Department of Human Molecular Genetics and Biochemistry , Sackler School of
Medicine , Tel Aviv , Israel.;
bxj University of Toyama, Division of Natural Drug Discovery, Institute of Natural
Medicine , Toyama , Japan.; on Hampton University , Department of Pharmaceutical
Sciences , School of Pharmacy , Hampton , VA , USA.; uv Istituto Giannina Gaslini,
UOC Medical Genetics , Genova , Italy.; ab Albert Einstein College of Medicine ,
Department of Molecular Pharmacology , Bronx , NY , USA.; bvi University of
Sydney , Department of Pathology and Bosch Institute , Sydney, New South Wales ,
Australia.; za Kyungpook National University , Department of Physiology , School of
Medicine , Jung-gu, Daegu , Korea.; os Hanyang University, College of Pharmacy ,
Ansan , Korea.; cck Yonsei University, College of Medicine, Severance Biomedical
Science Institute , Seoul , Korea.; bnm University of Massachusetts, Medical School
, Department of Molecular , Cell and Cancer Biology , Worcester , MA , USA.; q Ajou
University, College of Pharmacy , Gyeonggido , Korea.; azp Université Montpellier
2, Institut des Sciences de l'Evolution - UMR CNRS 5554 , Montpellier, Languedoc-
Roussillon , France.; b A. Mickiewicz University, Department of General Botany,
Institute of Experimental Biology, Faculty of Biology , Poznań , Poland.; cl Brown
University , Department of Ecology and Evolutionary Biology , Providence , RI ,
USA.; yq Kunming University of Science and Technology, Medical School , Kunmimg,
Yunnan , China.; aqo State Key Laboratory of Kidney Diseases, National Clinical
Research Center for Kidney Diseases , Department of Nephrology , Chinese PLA
General Hospital, Chinese PLA Institute of Nephrology , Beijing , China.; agz INCI,
CNRS UPR3212, Institut des Neurosciences Cellulaires and Intégratives ,
Strasbourg , France.; re Indian Institute of Science , Department of Microbiology
and Cell Biology , Bangalore , India.; bxz University of Urbino "Carlo Bo" ,
Department of Biomolecular Sciences , Urbino , Italy.; ld Federico II University,
Telethon Institute of Genetics and Medicine (TIGEM) , Department of Medical and
Translational Sciences , Naples , Italy.; bmu University of Malta , Department of
Physiology and Biochemistry , Faculty of Medicine and Surgery , Msida , Malta.; ie
CSIR, Indian Institute of Chemical Technology, Biomaterials Group , Hyderabad ,
India.; anl Semmelweis University , Department of Medical Chemistry , Molecular
Biology and Pathobioch mistry , Budapest , Hungary.; ccc Xuzhou Medical College ,
Department of Pathology , Xuzhou, Jiangsu , China.; azk Université du Québec à
Montréal , Département des Sciences Biologiques and Centre de Recherche BioMed ,
Montréal, Québec , Canada.; bye University of Valencia , Department of Pharmacology
, Valencia , Spain.; qy IMIM-Hospital del Mar CIBERES, Pompeu Fabra University,
Barcelona Biomedical Research Park , Respiratory Medicine Department , Lung Cancer
and Muscle Research Group , Barcelona , Spain.; ali Rice University , Department of
BioSciences , Houston , TX , USA.; hn Columbia University , Department of
Medicine , New York , NY , USA.; ud Iowa State University , Department of
Genetics , Development and Cell Biology , Ames , IA , USA.; anf Scientific
Institute IRCCS Eugenio Medea, Laboratory of Molecular Biology , Bosisio Parini,
Lecco , Italy.; avy University of Texas, MD Anderson Cancer Center , Department of
Experimental Therapeutics , Houston , TX , USA.; bqd University of North Texas
Health Science Center , Department of Molecular and Medical Genetics , Fort Worth ,
TX , USA.; bhr University of Coimbra , Coimbra , Portugal.; ayj Université
Catholique de Louvain (UCL), Institut des Sciences de la Vie , Louvain-la-Neuve ,
Belgium.; akh Polytechnic University of Marche , Department of Clinical Science ,
Faculty of Medicine , Ancona , Italy.; cbe Washington University, School of
Medicine, Departments of Obstetrics and Gynecology, and Pathology and Immunology ,
St. Louis , MO , USA.; buk University of South Carolina Upstate , Department of
Biology , Division of Natural Sciences and Engineering , Spartanburg, SC.; bih
University of Colorado, School of Medicine , Department of Pharmacology , Aurora ,
CO , USA.; bgs University of Cambridge, Division of Virology , Department of
Pathology , Cambridge , UK.; azh Université de Sherbrooke , Department of Anatomy
and Cell Biology , Faculty of Medicine and Health Sciences , Sherbrooke, QC ,
Canada.; av Atlanta Department of Veterans Affairs Medical Center , Decatur , GA.;
kh Emory University, Division of Endocrinology, Metabolism, and Lipids , Department
of Medicine , Atlanta , GA , USA.; lx Friedrich-Alexander-University Erlangen-
Nürnberg , Department of Medicine 1 , Erlangen , Germany.; bii University of
Colorado, School of Medicine, Division of Infectious Diseases , Aurora , CO , USA.;
abu McMaster University , Department of Biology , Hamilton, Ontario , Canada.; ko
Ernst-Moritz-Arndt University, Institute of Pharmacy , Greifswald , Germany.; aqq
State University of New York, College of Nanoscale Science and Engineering , Albany
, NY , USA.; bbo University Medical Center of the Johannes Gutenberg-University,
Institute for Pathobiochemistry , Mainz , Germany.; acc Medical School Goethe
University, Institute of Biochemistry II , Frankfurt , Germany.; op Hannover
Medical School , Department for Clinical Immunology and Rheumotology , Hannover ,
Germany.; bjo University of Florida , Department of Surgery , Gainesville , FL ,
USA.; z Albert Einstein College of Medicine , Department of Developmental and
Molecular Biology , Institute for Aging Studies , Bronx , NY , USA.; rv INSERM
U1081, CNRS UMR7284, Institute of Research on Cancer and Ageing of Nice (IRCAN) ,
Nice , France.; ana Sapienza University of Rome , Department of Clinical and
Molecular Medicine , Rome , Italy.; sc INSERM U862, Neurocentre Magendie , Bordeaux
, France.; aad Luxembourg Institute of Health and Centre Hospitalier de
Luxembourg , Luxembourg.; aku Queen Mary University of London, Blizard Institute,
Centre for Cell Biology and Cutaneous Research , London , UK.; baw University
Hospital Cologne, CECAD Research Center , Cologne , Germany.; bck University of
Amsterdam, Laboratory of Experimental Virology, Center for Infection and Immunity
Amsterdam (CINIMA), Academic Medical Center (AMC) , Amsterdam , The Netherlands.;
aal Magna Graecia University , Department of Health Sciences , Catanzaro , Italy.;
bog University of Michigan, Life Sciences Institute , Ann Arbor , MI , USA.; azb
Université de M ntréal , Department of Medicine , Montréal, Quebec , Canada.; bth
University of Rome "Tor Vergata" , Department of Experimental Medicine and
Surgery , Rome , Italy.; adk MRC Laboratory of Molecular Biology , Cambridge , UK.;
jl Duke University, Nicholas School of the Environment , Durham , NC , USA.; ayy
Université de Montpellier, DIMNP, UMR 5235, CNRS , Montpellier , France.; brh
University of Parma , Department of Biomedicine , Biotechnology and Translational
Research , Parma , Italy.; aic Northwestern University, Division of
Hematology/Oncology , Chicago , IL , USA.; akk Post Graduate Institute of Medical
Education and Research (PGIMER) , Department of Biophysics , Chandigarh , India.;
aff National Institute of Technology Rourkela , Department of Life Science ,
Rourkela, Odisha , India.; aro Technical University Munich, Institute of Human
Genetics , Munich, Bavaria , Germany.; sz Institute for Integrative Biology of the
Cell, Université Paris-Saclay , Gif-sur-Yvette , France.; azz Université Paris-Est
Créteil , Créteil , France.; bac Université Paris-Sud, CEA, CNRS , Paris , France.;
hb CNRS UM, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé ,
Montpellier , France.; kl Eötvös Loránd University , Department of Genetics ,
Budapest , Hungary.; axn Universidade Federal de São Paulo (UNIFESP) , Departamento
de Farmacologia , Escola Paulista de Medicina , São Paulo, SP , Brazil.; ml
Genentech Inc. , Department of Neuroscience , South San Francisco , CA , USA.; aqj
Stanford University , Department of Microbiology and Immunology , Stanford , CA ,
USA.; apt Sorbonne Universités, UPMC Univ Paris 06, INSERM UMRS974, CNRS FRE 3617,
Center for Research in Myology , Paris , France.; avj UCL Cancer Institute , London
, UK.; afk National Institutes of Health, Cell Biology Section, Neurogenetics
Branch, National Institute of Neurological Disorders and Stroke , Bethesda , MD ,
USA.; asm The Feinstein Institute for Medical Research, Laboratory of Developmental
Erythropoiesis , Manhasset , NY.; ben University of Buenos Aires, IDEHU-CONICET,
Faculty of Pharmacy and Biochemistry , Buenos Aires , Argentina.; bqr University of
Oslo, Institute of Basic Medical Sciences , Oslo , Norway.; aww Universidad de
Salamanca, Campus Unamuno, Instituto de Biologia Molecular y Celular del Cancer
(IBMCC), Centro de Investigacion del Cancer , Salamanca , Spain.; bdh University of
Bayreuth, Cell Biology , Bayreuth , Germany.; bbs University Medical Centre Utrecht
, Laboratory of Translational Immunology and Department of Pediatric Immunology ,
Utrecht , The Netherlands.; bro University of Pennsylvania , Department of
Biochemistry , SDM , Philadelphia , PA , USA.; jz Emory University , Department of
Cell Biology , Atlanta , GA , USA.; ka Emory University , Department of Hematology
and Medical Oncology , Atlanta , GA , USA.; cai INSPE, Institute of Experimental
Neurology, Division of Neuroscience , San Raffaele Scientific Institute, Milan ,
Italy.; zq Linköping University, Experimental Pathology , Department of Clinical
and Experimental Medicine , Faculty of Health Sciences , Linköping , Sweden.; brc
University of Padova , Department of Molecular Medicine , Padova , Italy.; ahm New
York University , Department of Psychiatry , New York NY ; and Center for Dementia
Research, Nathan S. Kline Institute , Orangeburg , NY , USA.; wj Johns Hopkins,
Bloomberg School of Public Health , Department of Biochemistry
and Molecular Biology and Johns Hopkins Malaria Research Institute , Baltimore ,
MD , USA.; axh Universidade de Santiago de Compostela , Departamento Farmacoloxía ,
Facultade de Veterinaria , Lugo , Spain.; azw Université Paris Descartes-Sorbonne
Paris Cité, Institut Necker Enfants-Malades (INEM), INSERM U1151-CNRS UMR 8253 ,
Paris , France.; bba University Hospital La Coruña , Microbiology Department , La
Coruña , Spain.; amx Sapienza University of Rome, DAHFMO-Section of Histology ,
Rome , Italy.; aye Université Bordeaux Segalen, Institut de Biochimie et Génétique
Cellulaires, CNRS UMR 5095 , Bordeaux , France ; buc University of Sherbrooke,
Faculté de Médecine et des Sciences de la Santé , Department of
Medicine/Gastroenterology Division , Sherbrooke, Québec , Canada.; rm Indiana
University School of Medicine , Department of Ophthalmology , Indianapolis , IN ,
USA.; bvb University of Southern California, The Saban Research Institute,
Developmental Neuroscience Program, Children's Hospital Los Angeles , Los Angeles ,
CA , USA.; ec Centro de Investigaciones Biológicas (CSIC) , Department of Cellular
and Molecular Biology , Madrid , Spain.; ays Université de Franche-Comté, UFR
Sciences et Techniques, Laboratoire de Biochimie , Besançon , France.; aqw Swedish
University of Agricultural Sciences and Linnean Center for Plant Biology,
Department of Chemistry and Biotechnology, Uppsala BioCenter, Uppsala, Sweden.; nj
German Cancer Research Center (DKFZ), Systems Biology of Cell Death Mechanisms ,
Heidelberg , Germany.; ra Imperial College London, National Heart and Lung
Institute , London , UK.; bxu University of Udine , Dipartimento di Scienze Mediche
e Biologiche , Udine , Italy.; mo Georg-August-Universität Göttingen , Department
of Molecular Microbiology and Genetics , Institute of Microbiology and Genetics ,
Göttingen , Germany.; km Centre de Recherche des Cordeliers, Equipe 11 labellisée
par la Ligue Nationale contre le Cancer , Paris , France.; oc Gustave Roussy Cancer
Campus , Villejuif , France.; sn INSERM, U1138 , Paris , France.; azv Université
Paris Descartes/Paris V , Paris , France.; lj Florida Atlantic University, Schmidt
College of Medicine , Department of Biomedical Sciences , Boca Raton , FL , USA.;
byt University of Wisconsin, School of Medicine and Public Health , Department of
Cell and Regenerative Biology , Carbone Cancer Center , Madison , WI , USA.; rv
INSERM U1081, CNRS UMR7284, Institute of Research on Cancer and Ageing of Nice
(IRCAN) , Nice , France.; bvo University of Tennessee Health Science Center ,
Department of Physiology , Memphis , TN , USA.; dt UMR 1324 INRA, 6265 CNRS,
Université de Bourgogne Franche-Comté, Centre des Sciences du Goût et de
l'Alimentation, Dijon , France.; brb University of Padova , Department of Biology ,
Padova , Italy.; axk Universidade de São Paulo, Instituto do Cancer do Estado de
São Paulo, Faculdade de Medicina , São Paulo, SP , Brazil.; xg Karolinska
Institute, Cancer Center Karolinska , Department of Oncology-Pathology ,
Stockholm , Sweden.; bsz University of Rochester Medical Center , Department of
Anesthesiology , Rochester , NY , USA.; mn Genentech Inc., Immunology and
Infectious Diseases , South San Francisco , CA , USA.; blz University of
Leicester , Department of Cancer Studies , Leicester , UK.; rl Indiana University
School of Medicine , Department of Microbiology and Immunology , Indianapolis ,
IN , USA.; rr INRA, UMR 1019 Nutrition Humaine , Centre de Clermont Theix, Saint
Genès Champanelle , France.; aym Université Clermont 1, UFR Médecine, UMR1019
Nutrition Humaine , Clermont-Ferrand , France.; btu University of São Paulo, School
of Physical Education and Sport, Cellular and Molecular Exercise Physiology
Laboratory , São Paulo , Brazil.; qd Hospital for Sick Children, Toronto , ON ,
Canada.; lc Federico II University , Department of Translational Medicine ,
Naples , Italy.; asa Telethon Institute of Genetics and Medicine (TIGEM) ,
Pozzuoli, Naples , Italy.; ada Monash University, School of Biological Sciences ,
Melbourne , Victoria , Australia.; bpi University of Nebraska Medical Center ,
Omaha , NE , USA.; bqy University of Oxford, Acute Stroke Programme, Radcliffe
Department of Medicine , Oxford , UK.; amh Sabanci University, Molecular Biology,
Genetics and Bioengineering Program , Istanbul , Turkey.; dn Centre Antoine
Lacassagne , Nice , France.; sk INSERM, U1081-UMR CNRS 7284 , Nice , France.; bpu
University of Nice-Sophia Antipolis, Institute for Research on Cancer and Aging of
Nice (IRCAN) , Nice , France.; bpx University of North Carolina , Department of
Genetics , Chapel Hill , NC , USA.; yo KU Leuven, Laboratory of Molecular and
Cellular Signaling , Department of Cellular and Molecular Medicine , Leuven ,
Belgium.; bdn University of Belgrade, School of Medicine, Institute of Histology
and Embryology , Belgrade , Serbia.; azd Université de Montréal, Faculty of
Pharmacy , Montréal, Québec , Canada.; hh Columbia University Medical Center ,
Department of Neurology , New York , NY , USA.; hi Columbia University Medical
Center , Department of Pathology and Cell Biology , New York , NY , USA.; boh
University of Michigan, Molecular and Behavioral Neuroscience Institute ,
Departments of Computational Medicine and Bioinformatics , Psychiatry, and Human
Genetics , Ann Arbor , MI , USA.; bdq University of Bern, Institute of Biochemistry
and Molecular Medicine , Bern , Switzerland.; bxk University of Trento, The
Microsoft Research, Centre for Computational and Systems Biology (COSBI) , Rovereto
, TN , Italy.; ahl New York University School of Medicine, Skirball Institute ,
Department of Microbiology , New York , NY , USA.; dh Center of Experimental
Medicine, Institute for Clinical and Experimental Medicine , Prague , Czech
Republic.; x Albert Einstein College of Medicine , Bronx , NY , USA.; cbs Wenzhou
Medical University, School of Optometry and Ophthalmology and Eye Hospital ,
Wenzhou, Zhejiang , China.; amd Rutgers University, The State University of New
Jersey , Department of Cell Biology and Neuroscience , Piscataway , NJ , USA.; bye
University of Valencia , Department of Pharmacology , Valencia , Spain.; ayq
Université de Bordeaux, UMR 5095, CNRS, Institut de Biochimie et génétique
Cellulaires , Bordeaux , France.; bmj University of London , RVC Department of
Comparative Biomedical Sciences , UCL Consortium for Mitochondrial Research ,
London , UK.; bfq University of California San Diego , Department of Pediatrics ,
La Jolla , CA , USA.; ava Trinity College Dublin, Smurfit Institute of Genetics ,
Dublin , Ireland.; uj IRCCS Santa Lucia Foundation , Department of Experimental
Neurosciences , Rome , Italy.; btd University of Rome "Tor Vergata" , Department of
Biology , Rome , Italy.; bpa University of Montpellier, UMR 866, Dynamique
Musculaire et Métabolisme , Montpellier , France.; bxg University of
Toronto/Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital , Department
of Obstetrics and Gynecology , Toronto, Ontario , Canada.; un IRCCS-Istituto di
Ricerche Farmacologiche Mario Negri , Department of Molecular Biochemistry and
Pharmacology , Milan , Italy.; dl Central South University , Department of
Pediatrics , Xiangya Hospital , Changsha, Hunan , China.; bkr University of Idaho,
Plant, Soil, and Entomological Sciences , Moscow , ID , USA.; ank Second University
of Naples , Department of Biochemistry , Biophysics and General Pathology ,
Naples , Italy.; anc Sapienza University of Rome , Department of Molecular Medicine
, Rome , Italy.; bhs University of Coimbra, Faculty of Medicine, Center for
Neuroscience and Cell Biology , Coimbra , Portugal.; gz Cleveland Clinic, Taussig
Cancer Institute , Cleveland , OH , USA.; agp National University of Ireland,
Apoptosis Research Centre , Galway , Ireland.; cw Case Western Reserve University,
Molecular Biology and Microbiology , Cleveland , OH , USA.; bxz University of
Urbino "Carlo Bo" , Department of Biomolecular Sciences , Urbino , Italy.; avs Umeå
University , Department of Medical Biochemistry and Biophysics , Umeå , Sweden.;
bke University of Graz, Institute of Molecular Biosciences, BioTechMed Graz ,
Graz , Austria.; kz Federal University of Rio de Janeiro, Insititute of
Microbiology , Department of Immunology , Rio de Janeiro , Brazil.; aki Polytechnic
University of Marche , Department of Life and Environmental Sciences , Ancona ,
Italy.; axr Universita' degli Studi di Modena e Reggio Emilia , Dipartimento di
Scienze Biomediche , Metaboliche e Neuroscienze , Modena , Italy.; dp Centre de
Recherche en Cancérologie de Marseille (CRCM), INSERM U1068, CNRS UMR 7258, Aix-
Marseille Université , Institut Paoli-Calmette, Parc Scientifique et Technologique
de Luminy, Marseille , France.; ahp ewcastle University, Campus for Ageing and
Vitality, Institute for Cell and Molecular Biosciences and Institute for Ageing ,
Newcastle upon Tyne , UK.; axx Universitat Autònoma de Barcelona , Department of
Cell Biology , Physiology and Immunology, Institut de Neurociències , Barcelona ,
Spain.; apx Spanish Council for Scientific Research, Institute for Advanced
Chemistry of Catalonia , Department of Biomedicinal Chemistry , Barcelona , Spain.;
ll Fondazione IRCCS Istituto Nazionale dei Tumori , Department of Experimental
Oncology and Molecular Medicine , Milan , Italy.; bdf University of Basel ,
Biozentrum, Basel, BS , Switzerland.; hf Colonia Ciudad Universitaria ,
Neurodevelopment and Physiology Department , Neuroscience Division, Instituto de
Fisiologia Celular, UNAM , Mexico , DF , Mexico.; bru University of Pisa,
Interdepartmental Research Centre on Biology and Pathology of Aging , Pisa ,
Italy.; uv Istituto Giannina Gaslini, UOC Medical Genetics , Genova , Italy.; is
Danish Cancer Society Research Center , Unit of Cell Stress and Survival (CSS) ,
Copenhagen , Denmark.; ui IRCCS Santa Lucia Foundation
, Rome , Italy.; btl University of Rome "Tor Vergata" , Department of Biology,
Rome , Italy.; qq Icahn School of Medicine at Mount Sinai , Department of
Pharmacology and Systems Therapeutics , New York , NY , USA.; gq CIBERNED, ISCIII,
Unidad Asociada Neurodeath , Madrid , Spain.; awg Universidad de Castilla-La Mancha
, Albacete , Spain.; axw Università Vita-Salute San Raffaele , Milan , Italy.; caj
San Raffaele Scientific Institute , Milan , Italy.; qb Hôpital Kirchberg,
Laboratoire de Biologie Moléculaire et Cellulaire du Cancer , Luxembourg.; bxt
University of Tuscia , Department for Innovation in Biological , Agro-food and
Forest Systems (DIBAF) , Viterbo , Italy.; bdv University of Bologna , Dipartimento
di Scienze Biomediche e Neuromotorie , Bologna , Italy.; byz University of Zürich ,
Department of Radiation Oncology , Zurich , Switzerland.; ge Chonbuk National
University , Department of Pharmacology , Medical School , Chonbuk , Korea.; xj
Karolinska Institute , Department of Physiology and Pharmacology , Stockholm ,
Sweden.; xb Kaohsiung Medical University Hospital , Department of Pathology ,
Kaohsiung City , Taiwan.; xd Kaohsiung Medical University, Faculty of Medicine ,
Department of Pathology , Kaohsiung City , Taiwan.; bet University of Calcutta ,
Department of Biotechnology , Dr.B.C. Guha Centre for Genetic Engineering and
Biotechnology , Kolkata , WB , India.; bio University of Crete, School of
Medicine , Department of Infectious Diseases , Heraklion, Crete , Greece.; aqx
Strathclyde Institute of Pharmacy and Biomedical Sciences , Glasgow , UK.; fy
Chinese University of Hong Kong , Department of Anaesthesia and Intensive Care ,
Shatin, NT, Hong Kong.; alo Roswell Park Cancer Institute , Department of
Pharmacology and Therapeutics , Buffalo , NY , USA.; ub International Center for
Genetic Engineering and Biotechnology, Immunology Group , New Delhi , India.; ael
National Cheng Kung University , Department of Microbiology and Immunology ,
College of Medicine , Tainan , Taiwan.; bko University of Hong Kong, Laboratory of
Neurodegenerative Diseases , School of Biomedical Sciences , LKS Faculty of
Medicine , Hong Kong , China.; mg Geisel School of Medicine at Dartmouth ,
Department of Biochemistry , Hanover , NH , USA.; bcd University of Alabama at
Birmingham, Division of Molecular and Cellular Pathology , Department of
Pathology , Birmingham , AL , USA.; bul University of South Carolina, Environmental
Health and Disease Laboratory , Department of Environmental Health Sciences ,
Columbia , SC , USA.; tg Institute of Life Sciences , Bhubaneshwar , Odisa ,
India.; bj Beijing Institute of Pharmacology and Toxicology, State Key Laboratory
of Toxicology and Medical Countermeasures , Beijing , China.; avo UCL Institute of
Ophthalmology , London , UK.; bpo University of New South Wales, Inflammation and
Infection Research Centre, School of Medical Sciences , Sydney, NSW , Australia.;
ari Taichung Veterans Genera Hospital , Department of Medical Research , Taichung
City , Taiwan.; vz Jinshan Hospital of Fudan University , Department of Urology ,
Shanghai , China.; bll University of Kentucky, College of Medicine , Department of
Pharmacology and Nutritional Sciences , Lexington , KY , USA.; i Academia Sinica,
Institute of Biological Chemistry , Taipei , Taiwan.; aol Shanghai Jiao Tong
University, School of Medicine, Key Laboratory of Cell Differentiation and
Apoptosis of Chinese Ministry of Education , Shanghai , China.; aok Shanghai Jiao
Tong University, School of Medicine , Department of Pharmacology and Chemical
Biology , Shanghai , China.; bff University of California Irvine , Department of
Neurosurgery , Irvine , CA , USA.; nf Georgia Regents University, Medical College
of Georgia , Department of Cellular Biology and Anatomy , Augusta , GA , USA.; ng
Georgia Regents University, Medical College of Georgia , Department of Medicine ,
Augusta , GA , USA.; io Dalian Medical University , Department of Food Nutrition
and Safety , Dalian , China.; cbx Wuhan University, College of Life Science, State
Key Laboratory of Virology , Wuhan, Hubei , China.; brc University of Padova ,
Department of Molecular Medicine , Padova , Italy.; blj University of Kansas
Medical Center , Department of Pharmacology , Toxicology and Therapeutics , Kansas
City , KS , USA.; fp Chinese Academy of Sciences, Institute of Zoology , Beijing ,
China.; eh Chang Gung University, College of Medicine , Department of Neurology ,
Kaohsiung Chang Gung Memorial Hospital , Kaohsiung , Taiwan.; lm Food and Drug
Administration (FDA), Division of Biochemical Toxicology, National Center for
Toxicological Research (NCTR) , Jefferson , AR , USA.; j Academia Sinica, Institute
of Biomedical Sciences , Taipei , Taiwan.; ccs Zhejiang University , Department of
Food Science and Nutrition , Hangzhou , China.; aew National Ilan University ,
Department of Biotechnology and Animal Science , Yilan City , Taiwan.; akq Qilu
Hospital of Shandong University, Cardiology , Jinan, Shandong , China.; apu South
China Normal University, College of Biophotonics , Guangdong , China.; aqo State
Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney
Diseases , Department of Nephrology , Chinese PLA General Hospital, Chinese PLA
Institute of Nephrology , Beijing , China.; arm Tamkang University , Department of
Chemistry , Tamsui, New Taipei City , Taiwan.; avb Tsinghua University, School of
Life Sciences , Beijing , China.; bcq University of Arizona , Department of
Pharmacology and Toxicology , College of Pharmacy , Tucson , AZ , USA.; ajq Peking
University , Department of Immunology , Beijing , China.; ajs Peking University,
Health Science Center, Center for Human Disease Genomics , Beijing , China.; cdc
Zhengzhou University Affiliated Cancer Hospital , Zhengzhou , China.; aaj Mackay
Memorial Hospital , Department of Radiation Oncology , Taipei , Taiwan.; ara Sun
Yat-Sen University, Key Laboratory of Gene Engineering of the Ministry of
Education, School of Life Science , Guangzhou , China.; atl Third Military Medical
University , Department of Neurosurgery , Southwest Hospital , Shapingba District,
Chongqing , China.; ma Fudan University, Cancer Center , Department of Integrative
Oncology , Shanghai , China.; ccq Zhejiang University, Deparment of Pharmacology,
College of Pharmaceutical Sciences , Hangzhou, Zhejiang , China.; bml University of
Louisville , Department of Biochemistry and Molecular Genetics , Louisville , KY ,
USA.; gb Chinese University of Hong Kong, School of Biomedical Sciences, Faculty of
Medicine , Shatin, NT, Hong Kong.; bnl University of Maryland, School of Medicine,
Institute of Human Virology , Baltimore , MD , USA.; aeh National Cancer Center,
Division of Cancer Biology, Research Institute , Gyeonggi , Korea.; brp University
of Pennsylvania , Department of Microbiology , Philadelphia , PA , USA.; bmm
University of Louisville , Department of Medicine (Hem-Onc) , Louisville , KY ,
USA.; aek National Cheng Kung University, College of Medicine , Department of
Pharmacology and Institute of Basic Medical Sciences , Tainan , Taiwan.; azg
Université de Rennes-1, Oncogenesis, stress, Signaling" (OSS), ERL 440 INSERM,
Centre de Lutte Contre le Cancer Eugene Marquis , Rennes , France.; ej Chang Gung
University , Department of Biochemistry , College of Medicine , Taoyuan , Taiwan.;
yf Korea University , Department of Life Science and Biotechnology , Seoul ,
Korea.; kv European Institute of Oncology (IEO) , Department of Experimental
Oncology , Milan , Italy.; ajv Pennsylvania State University, College of Medicine ,
Department of Cellular and Molecular Physiology , Hershey , PA , USA.; brf
University of Palermo , Dipartimento di Scienze e Tecnologie Biologiche , Chimiche
e Farmaceutiche (STEBICEF) , Palermo , Italy.; ia Consiglio Nazionale delle
Ricerche, Core Research Laboratory , Siena , Italy.; uu Istituto di Fisiologia
Clinica , Siena , Italy.; ve Istituto Toscano Tumori , Siena , Italy.; afc National
Institute of Gastoenterology, Laboratory of Experimental Immunopathology ,
Castellana Grotte (BA) , Italy.; kd Emory University, School of Medicine ,
Department of Pharmacology , Atlanta , GA , USA.; awk Universidad de Chile,
Advanced Center for Chronic Diseases (ACCDiS) , Santiago , Chile.; agt National
University of Singapore , Department of Pharmacy , Singapore.; yz Kyung Hee
University, Graduate School of East-West Medical Science , Seoul , Korea.; xz
Konkuk University , Department of Animal Biotechnology , Seoul , Korea.; akt Queen
Elizabeth Hospital , Department of Clinical Oncology , Kowloon, Hong Kong.; da
Catholic University of Korea, College of Pharmacy , Bucheon , Korea.; anx Seoul St.
Mary's Hospital , Department of Internal Medicine , Seoul, Korea.; cbo Weill
Cornell Medical College , New York , NY , USA.; yf Korea University , Department of
Life Science and Biotechnology , Seoul , Korea.; nw Graduate School of Hallym
University , Chuncheon, Kangwon-do , Korea.; oj Hallym University , Department of
Biomedical Gerontology , Chuncheon, Kangwon-do, Korea; and Anyang, Gyeonggi-do ,
Korea.; zi Laboratory of Cellular Aging and Neurodegeneration, Ilsong Institute of
Life Science, Anyang , Gyeonggi-do , Korea.; bhc University of Chicago , Department
of Pathology , Chicago , IL , USA.; cbm Weill Cornell Medical College, Division of
Nephrology and Hypertension, Joan and Sanford I . Weill Department of Medicine ,
New York , NY , USA.; ccj Yonsei University, College of Medicine, Corneal Dystrophy
Research Institute ; and Department of Ophthalmology , Seoul , Korea.; ov Harbor-
UCLA Medical Center and Los Angeles Biomedical Research Institute, Division of
Medical Genetics , Department of Pediatrics , Torrance , CA , USA.; oe Gustave
Roussy Institute , Villejuif , France.; bhn University of Cincinnati , Cincinnati ,
OH , USA.; bvl University of Tartu
, Department of Pharmacology , Tartu , Estonia.; aep National Chung Hsing
University, Graduate Institute of Biomedical Sciences , Taichung , Taiwan.; abb Max
Planck Institute of Biophysical Chemistry , Department of Molecular Cell Biology ,
Göttingen , Germany.; bsj University of Pittsburgh, School of Medicine , Department
of Pathology and Center for Neuroscience , Pittsburgh , PA , USA.; aeu National
Health Research Institutes, Immunology Research Center , Miaoli , Taiwan.; yg Korea
University, Department of Biotechnology, College of Life Sciences and Biotechnology
, Seoul , Korea.; yb Konkuk University School of Medicine , Department of
Ophthalmology , Seoul , Korea.; akp Pusan National University , Department of
Biological Sciences , Busan , Korea.; asx The Institute of Cancer Research, Cancer
Research UK Cancer Imaging Centre, Division of Radiotherapy and Imaging , Sutton,
Surrey , UK.; r Ajou University, School of Medicine , Department of Microbiology ,
Gyeonggi-do , Korea.; it Danish Cancer Society Research Center, Unit of Cell Stress
and Survival , Copenhagen , Denmark.; bpg University of Naples Federico II ,
Department of Veterinary Medicine and Animal Production , Naples , Italy.; ahb
Nencki Institute of Experimental Biology, Neurobiology Center, Laboratory of
Molecular Neurobiology , Warsaw , Poland.; btd University of Rome "Tor Vergata" ,
Department of Biology , Rome , Italy.; anb Sapienza University of Rome , Department
of Experimental Medicine , Rome , Italy.; bmd KU Leuven-University of Leuven,
Center for Human Genetics; VIB Center for the Biology of Disease , Leuven ,
Belgium.; bmh University of Liverpool, Cellular and Molecular Physiology, Institute
of Translational Medicine , Liverpool , UK.; bda University of Barcelona ,
Department of Biochemistry and Molecular Genetics , Hospital Clínic, IDIBAPS-
CIBERehd , Barcelona , Spain.; blw University of Lausanne , Department of
Fundamental Neurosciences , Faculty of Biology and Medicine , Lausanne ,
Switzerland.; mw Georgetown University Medical Center , Department of Oncology ,
Washington, DC , USA.; ane Scientific Institute IRCCS Eugenio Medea , Bosisio
Parini , Italy.; bat University Hospital "Luigi Sacco", Università di Milano, Unit
of Clinical Pharmacology, National Research Council-Institute of Neuroscience ,
Department of Biomedical and Clinical Sciences "Luigi Sacco" , Milano , Italy.; bpm
University of New Mexico , Department of Pathology and Cancer Research and
Treatment Center , Albuquerque , NM , USA.; azn Universite Libre de Bruxelles, ULB
Center for Diabetes Research , Brussels , Belgium.; vb Istituto Superiore di Sanità
, Department of Infectious , Parasitic and Immunomediated Diseases , Rome , Italy.;
bdc University of Bari 'Aldo Moro' , Department of Basic Medical Sciences ,
Neurosciences and Organs of Senses , Bari , Italy.; azw Université Paris Descartes-
Sorbonne Paris Cité, Institut Necker Enfants-Malades (INEM), INSERM U1151-CNRS UMR
8253 , Paris , France.; jk Duke University, Medical Center , Department of
Molecular Genetics and Microbiology , Durham , NC , USA.; bfy University of
California San Diego, Moores Cancer Center , La Jolla , CA , USA.; ia Consiglio
Nazionale delle Ricerche, Core Research Laboratory , Siena , Italy.; uu Istituto di
Fisiologia Clinica , Siena , Italy.; ve Istituto Toscano Tumori , Siena , Italy.; y
Albert Einstein College of Medicine , Department of Developmental and Molecular
Biology , Bronx , NY , USA.; ds Centre for Research in Agricultural Genomics (CSIC-
IRTA-UAB-UB) , Bellaterra , Catalonia , Spain.; sv Institut Pasteur, CNRS, URA2578,
Unité Macrophages et Développement de l'Immunité , Département de Biologie du
Développement et des Cellules Souches , Paris , France.; bri University of Pavia ,
Department of Biology and Biotechnology , Pavia , Italy.; vf Italian National
Institute of Health , Department of Technology and Health, Rome , Italy.; cas Wake
Forest University , Department of Surgery , Hypertension and Vascular Research
Center, Wake Forest Comprehensive Cancer Center , Winston-Salem , NC , USA.; bve
University of Strathclyde, Strathclyde Institute of Pharmacy and Biomedical
Sciences , Glasgow , UK.; cav Washington State University Vancouver, School of
Molecular Biosciences , Vancouver , WA , USA.; baq University College London ,
Department of Clinical Neurosciences , London , UK.; wc Johns Hopkins University,
Bloomberg School of Public Health, Malaria Research Institute , Department of
Molecular Microbiology and Immunology , Baltimore , MD , USA.; bai University
"Magna Graecia" of Catanzaro , Department of Health Sciences , Catanzaro , Italy.;
rq INMI-IRCCS "L. Spallanzani" , Rome , Italy.; btl University of Rome "Tor
Vergata" , Department of Biology, Rome , Italy.; bhf University of Chile, Advanced
Center for Chronic Diseases (ACCDiS), Division of Cardiovascular Diseases, Faculty
of Medicine , Santiago , Chile.; iq Danish Cancer Society Research Center, Cell
Death and Metabolism Unit, Center for Autophagy, Recycling and Disease , Copenhagen
, Denmark.; bua University of Sevilla, Instituto de Biomedicina de Sevilla (IBIS) ,
Oral Medicine Department , Sevilla , Spain.; awo Universidad de Córdoba, Campus de
Excelencia Agroalimentario (ceiA3), Departamento de Genética , Córdoba , Spain.; sm
INSERM, U1127, CNRS, UMR 7225 , Paris , France.; apq Sorbonne Universités, UMR
S1127 , Paris , France.; boy University of Modena and Reggio Emilia, School of
Medicine , Department of Surgery , Medicine, Dentistry and Morphological Sciences ,
Modena , Italy.; bxq University of Turin , Department of Clinical and Biological
Sciences , Unit of Experimental Medicine and Clinical Pathology , Turin , Italy.;
bfj University of California Los Angeles, Larry Hillblom Islet Research Center,
David Geffen School of Medicine , Los Angeles , CA , USA.; aas Massachusetts
General Hospital and Harvard Medical School, Center for Human Genetic Research and
Department of Neurology , Boston , MA , USA.; ajj Oviedo University, Morphology and
Cellular Biology Department , Oviedo , Spain.; ut IRO, Institute for Research in
Ophthalmology , Sion , Switzerland.; blx University of Lausanne , Department of
Ophthalmology , Lausanne , Switzerland.; axa Universidad de Valparaíso, Instituto
de Biología, Facultad de Ciencias , Valparaíso , Chile.; ama Rutgers University ,
Department of Cell Biology and Neuroscience , Piscataway , NJ , USA.; ach Medical
University of South Carolina, Biochemistry and Molecular Biology , Charleston ,
SC , USA.; bgb University of California San Francisco , Department of Microbiology
and Immunology , San Francisco , CA , USA.; bbw University of Aberdeen, Division of
Applied Medicine , Aberdeen , UK.; brz University of Pittsburgh , Department of
Microbiology and Molecular Genetics , Pittsburgh , PA , USA.; buu University of
Southampton, Cancer Sciences , Southampton , UK.; blo University of Kentucky ,
Department of Pharmacology and Nutritional Sciences , Lexington , KY , USA.; bxs
University of Turin , Turin , Italy.; awz Universidad de Sevilla, Instituto de
Bioquímica Vegetal y Fotosíntesis, CSIC , Sevilla , Spain.; awk Universidad de
Chile, Advanced Center for Chronic Diseases (ACCDiS) , Santiago , Chile.; ul IRCCS,
"C. Mondino" National Neurological Institute, Experimental Neurobiology Lab , Pavia
, Italy.; bhp University of Coimbra, Center for Neuroscience and Cell Biology and
Faculty of Pharmacy , Coimbra , Portugal.; z Albert Einstein College of Medicine ,
Department of Developmental and Molecular Biology , Institute for Aging Studies ,
Bronx , NY , USA.; awc Universidad Autonoma de Madrid, Departamento de Biologia
Molecular , Madrid , Spain.; bui University of South Carolina School of Medicine ,
Department of Cell Biology and Anatomy , Columbia , SC , USA.; aky Queen Mary
University of London, Centre for Haemato-Oncology, Barts Cancer Institute ,
London , UK.; aa Albert Einstein College of Medicine , Department of Medicine ,
Bronx , NY , USA.; bhl University of Cincinnati College of Medicine , Department of
Cancer Biology , Cincinnati , OH , USA.; can University of Nevada School of
Medicine, Department of Pharmacology, Reno, NV, USA.; baz University Hospital
Jena , Department of General , Visceral and Vascular Surgery, Experimental
Transplantation Surgery , Jena , Germany.; adt Nanjing Medical University, Center
for Kidney Disease, 2nd Affiliated Hospital , Jiangsu , China.; asq The First
Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic
Surgery , Department of General Surgery , Harbin , China.; cae Virginia
Commonwealth University, Massey Cancer Center , Department of Medicine , Richmond ,
VA , USA.; bvp University of Texas at Austin, College of Pharmacy, Division of
Medicinal Chemistry , Austin , TX , USA.; brb University of Padova , Department of
Biology , Padova , Italy.; ayn Université d'Auvergne, M2iSH "Microbes, Intestine,
Inflammation, Susceptibility of the Host", UMR 1071 INSERM, Centre Biomédical de
Recherche et Valorisation, Faculté de Médecine , Clermont-Ferrand , France.; uk
IRCCS Santa Lucia Foundation , Rome , Italy.; gf Christian Albrechts University,
Institut für Biochemie , Kiel , Germany.; ayn Université d'Auvergne, M2iSH
"Microbes, Intestine, Inflammation, Susceptibility of the Host", UMR 1071 INSERM,
Centre Biomédical de Recherche et Valorisation, Faculté de Médecine , Clermont-
Ferrand , France.; ajn Paris Diderot University, Sorbonne Paris Cité, INSERM,
CNRS , Paris , France.; bcc University of Alabama at Birmingham , Department of
Pathology , Center for Free Radical Biology , Birmingham , AL , USA.; gw Cleveland
Clinic , Cleveland , OH , USA.; gt Cincinnati Children's Hospital Medical Center,
Division of Oncology , Cincinnati , OH , USA.; avf Tulane University Health
Sciences Center , Department of Pathology and Laboratory Medicine , New Orleans ,
LA , USA.;
ih Curtin University, School of Pharmacy , Bentley , Australia.; h Alberystwyth
University, Institute of Biological, Environmental and Rural Sciences , Penglais ,
Aberystwyth , Wales , UK.; bgz University of Cape Town, Redox Laboratory ,
Department of Human Biology , Cape Town , South Africa.; hs Complutense University,
Instituto de Investigaciones Sanitarias San Carlos (IdISSC) , Department of
Biochemistry and Molecular Biology I , School of Biology , Madrid , Spain.; bno
University of Massachusetts, Medical School, Howard Hughes Medical Institute ,
Worcester , MA , USA.; wf Johns Hopkins University, School of Medicine ,
Departments of Neurology , Neuroscience and Pharmacology and Molecular Sciences ;
Neuroregeneration Program, Institute for Cell Engineering , Baltimore , MD , USA.;
wh Johns Hopkins University, School of Medicine, Neuroregeneration and Stem Cell
Programs, Institute for Cell Engineering , Department of Neurology , Department of
Physiology , Baltimore , MD , USA.; btz University of Sevilla , Department of Cell
Biology , Sevilla , Spain.; rb Imperial College London, Neurogenetics Group,
Division of Brain Sciences , London , UK.; asj Texas A&M University , Department of
Microbial Pathogenesis and Immunology , Texas A&M Health Science Center , Bryan ,
TX , USA.; asl Texas A&M University, The Norman Borlaug Center , College Station ,
TX , USA.; ee Centro de Pesquisas Aggeu Magalhães/FIOCRUZ-PE , Departamento de
Microbiologia , Recife , PE , Brazil.; ami SaBio, Instituto de Investigación en
Recursos Cinegéticos IREC-CSIC-UCLM-JCCM , Ciudad Real , Spain.; bpg University of
Naples Federico II , Department of Veterinary Medicine and Animal Production ,
Naples , Italy.; asa Telethon Institute of Genetics and Medicine (TIGEM) ,
Pozzuoli, Naples , Italy.; bcm University of Antwerp, Laboratory of
Physiopharmacology, Wilrijk , Antwerp , Belgium.; xg Karolinska Institute, Cancer
Center Karolinska , Department of Oncology-Pathology , Stockholm , Sweden.; bxz
University of Urbino "Carlo Bo" , Department of Biomolecular Sciences , Urbino ,
Italy.; all Rio de Janeiro Federal University, Instituto de Biofísica Carlos Chagas
Filho , Rio de Janeiro , Brazil.; brt University of Pisa , Department of
Translational Research and New Technologies in Medicine and Surgery , Pisa ,
Italy.; ir Danish Cancer Society Research Center, Cell Stress and Survival Unit ,
Copenhagen , Denmark.; bgi University of California San Francisco, School of
Medicine , Department of Pathology , San Francisco , CA , USA.; kq ETH Zurich,
Institute of Biochemistry , Zurich , Switzerland.; yk KU Leuven , Department of
Abdominal Transplant Surgery , Leuven , Belgium.; bbh University Hospitals Leuven ,
Department of Microbiology and Immunology , Laboratory of Abdominal Transplantation
, Leuven , Belgium.; agp National University of Ireland, Apoptosis Research
Centre , Galway , Ireland.; ayp Université de Bordeaux, Institut des Maladies
Neurodégénératives, CNRS UMR 5293 , Bordeaux , France.; bud University of Siena ,
Department of Molecular and Developmental Medicine , Siena , Italy.; amc Rutgers
University, New Jersey Medical School , Department of Cell Biology and Molecular
Medicine , Newark , NJ , USA.; ayr Université de Franche-Comté, UFR Sciences et
Techniques EA3922/SFR IBCT FED 4234, Estrogènes, Expression Génique et Pathologies
du Système Nerveux Central , Besançon , France.; bns University of Melbourne ,
Department of Physiology , Parkville , Australia.; ayk Université Catholique de
Louvain (UCL), Institute of Neuroscience , Louvain-la-Neuve , Belgium.; bog Unive
sity of Michigan, Life Sciences Institute , Ann Arbor , MI , USA.; asb Temasek Life
Sciences Laboratory , Singapore.; bjr University of Freiburg , Department of
Dermatology , Medical Center, Center for Biological Systems Analysis (ZBSA) ,
Freiburg , Germany.; blt University of La Réunion, CYROI, IRG Immunopathology and
Infection Research Grouping , Reunion , France.; bzw Virginia Commonwealth
University , Department of Biochemistry and Molecular Biology , Richmond , VA ,
USA.; bpz University of North Carolina, Lineberger Comprehensive Cancer Center ,
Chapel Hill , NC , USA.; bpn University of New Mexico, Health Sciences Center ,
Department of Molecular Genetics and Microbiology , Albuquerque , NM , USA.; kt ETH
Zürich, LFW D 18.1 , Zürich , Switzerland.; bad Université Paris-Sud, INSERM 1030,
Gustave Roussy Cancer Campus , Paris , France.; asi Texas A&M University ,
Department of Biochemistry and Biophysics , College Station , TX , USA.; acx Monash
University, Clayton Campus , Department of Biochemistry and Molecular Biology ,
Melbourne , Victoria , Australia.; btd University of Rome "Tor Vergata" ,
Department of Biology , Rome , Italy.; btj University of Rome "Tor Vergata" ,
Department of System Medicine , Rome , Italy.; amz Sapienza University of Rome ,
Department of Biochemical Sciences "A. Rossi Fanelli" , Rome , Italy.; ew
Children's Hospital , Department of Neurology , Boston , MA , USA.; asa Telethon
Institute of Genetics and Medicine (TIGEM) , Pozzuoli, Naples , Italy.; awo
Universidad de Córdoba, Campus de Excelencia Agroalimentario (ceiA3), Departamento
de Genética , Córdoba , Spain.; anb Sapienza University of Rome , Department of
Experimental Medicine , Rome , Italy.; cbb Washington University, School of
Medicine , Department of Developmental Biology , St. Louis , MO , USA.; awl
Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas , Santos Dumont
, Santiago de Chile.; bcg University of Alcala , Department of System Biology ,
Biochemistry and Molecular Biology Unit, School of Medicine , Madrid , Spain.; amt
Sanford Burnham Prebys Medical Discovery Institute , La Jolla , CA , USA.; awa
Universidad Autónoma de Madrid, Centro de Biología Molecular Severo Ochoa,
CIBERER , Madrid , Spain.; bup University of South Florida, Byrd Alzheimer's
Institute , Tampa , FL , USA.; bln University of Kentucky , Department of Molecular
and Cellular Biochemistry , Lexington , KY , USA.; anu Seoul National University ,
College of Pharmacy , Seoul , Korea.; biz University of Edinburgh, Easter Bush, The
Roslin Insitute , Midlothian , UK.; nq Goethe University School of Medicine,
Institute of Biochemistry II and Buchmann Institute for Molecular Life Sciences ,
Frankfurt am Main , Germany.; bfc University of California Davis , Department of
Plant Biology and the Genome Center , College of Biological Sciences , Davis , CA ,
USA.; aov Shanghai Veterinary Research Institute , Shanghai , China.; blj
University of Kansas Medical Center , Department of Pharmacology , Toxicology and
Therapeutics , Kansas City , KS , USA.; bcr University of Arkansas for Medical
Sciences , Department of Cardiology , Little Rock , AR , USA.; btn University of
Salento , Department of Biological and Environmental Science and Technology , Lecce
, Italy.; bjc University of Erlangen-Nuremberg , Department of Internal Medicine
3 , Erlangen , Germany.; cbg Washington University, School of Medicine, John
Cochran VA Medical Center, Center for Cardiovascular Research , St. Louis , MO ,
USA.; ayo Université de Bordeaux, INSERM U916, Institut Bergonié , Bordeaux cedex ,
France.; aeb NAS of Ukraine , Department of Molecular Genetics and Biotechnology ,
Institute of Cell Biology , Lviv , Ukraine.; bgx University of Canterbury,
Biomolecular Interaction Centre, School of Biological Sciences , Christchurch , New
Zealand.; np Goethe University of Frankfurt, Institute of Biophysical Chemistry ,
Frankfurt am Main , Germany.; bpl University of New Mexico , Department of Internal
Medicine , Albuquerque , NM , USA.; bae Université Paris-Sud, Institut Gusta e
Roussy, CNRS UMR 8126 , Villejuif , France.; byg University of Verona , Department
of Neurological, Biomedical and Movement Sciences , Verona , Italy.; rj Indiana
University School of Medicine , Department of Biochemistry and Molecular Biology ,
Indianapolis , IN , USA.; bvr University of Texas , Southwestern Medical Center,
Department of Internal Medicine , Center for Autophagy Research, Dallas , TX ,
USA.; nf Georgia Regents University, Medical College of Georgia , Department of
Cellular Biology and Anatomy , Augusta , GA , USA.; bph University of Nebraska
Medical Center , Department of Internal Medicine , Omaha , NE , USA.; bzj VA
Nebraska-Western Iowa Health Care System , Omaha , NE , USA.; amm San Diego State
University , Department of Biology and Center for Microbial Sciences , San Diego ,
CA , USA.; bah University "G. dAnnunzio" , Department of Medical , Oral and
Biotechnological Sciences , Chieti , Italy.; cax Washington University in St.
Louis, School of Medicine , Department of Internal Medicine , St. Louis , MO ,
USA.; by Bogomoletz Institute of Physiology, National Academy of Sciences Ukraine ,
General and Molecular Pathophysiology Department , Kiev , Ukraine.; bcu University
of Arkansas, Center of Excellence for Poultry Science , Fayetteville , AR , USA.;
arx Tel Aviv University, Oncogenetic Laboratory, Meir Medical Center, Kfar Saba and
Sackler Faculty of Medicine , Tel Aviv , Israel.; bi Beijing Anzhen Hospital,
Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel
Diseases , Beijing , China.; afb National Institute of Biological Sciences ,
Beijing , China.; me Fujian Provincial Hospital, Department of Urology , Fuzhou ,
China.; aqr Stellenbosch University , Department of Physiological Sciences ,
Stellenbosch , South Africa.; akw Queen Mary University of London, Blizard
Institute , Department of Neuroscience and Trauma , London , UK.; alx Rush
University Medical Center , Department of Anatomy and Cell Biology , Chicago , IL ,
USA.; aim Ohio State University, DHLRI , Department of Medicine , Columbus , OH ,
USA.; rg Indian Institute of Technology Guwahati , Department of Biosciences and
Bioengineering , Guwahati , Assam , India.; bas University
College London, UCL Consortium for Mitochondrial Research and Department of Cell
and Developmental Biology , London , UK.; bbd University Hospital of Lausanne,
Service and Central Laboratory of Hematology , Lausanne , Switzerland.; bbk
University Lille, INSERM, CHU Lille, Institut Pasteur de Lille, U1011, EGID , Lille
, France.; si INSERM UMRS 1166, Unité de Recherche sur les Maladies
Cardiovasculaires, du Métabolisme et de la Nutrition , Paris , France.; lu Freiburg
University, Center for Biological Systems Analysis (ZBSA), Core Facility Proteomics
, Freiburg , Germany.; bnz University of Michigan , Department of Cell and
Developmental Biology , Ann Arbor , MI , USA.; ct Cardiff University, Institute of
Cancer and Genetics , Cardiff , Wales, UK.; bji University of Florida, College of
Medicine , Department of Anatomy and Cell Biology , Gainesville , FL , USA.; azw
Université Paris Descartes-Sorbonne Paris Cité, Institut Necker Enfants-Malades
(INEM), INSERM U1151-CNRS UMR 8253 , Paris , France.; ry INSERM U1118, Mécanismes
Centraux et Périphétiques de la Neurodégénérescence , Strasbourg , France.; azi
Université de Strasbourg, Faculté de Médecine, UMRS 1118 , Strasbourg , France.; se
INSERM U916, Université de Bordeaux, Institut Européen de Chimie et Biologie ,
Pessac , France.; abt McGill University, Montreal Neurological Institute ,
Montreal, QC , Canada.; ayg Université Bordeaux, CNRS, Institut de Biochimie et
Génétique Cellulaires, UMR 5095 , Bordeaux , France.; bzk VA Pittsburgh Health
System, University of Pittsburgh Medical Center , Pittsburgh , PA , USA.; cd
Brandeis University , Department of Biology , Waltham , MA , USA.; alw Ruprecht-
Karls-University Heidelberg, Division of Pediatric Neurology , Department of
Pediatrics , Heidelberg University Hospital , Heidelberg , Germany.; st Institut
Pasteur, CNRS URA2582 , Cell Biology and Infection Department , Membrane Traffic
and Cell Division Lab , Paris , France.; acn Medical University of Vienna ,
Department of Dermatology , Vienna , Austria.; bhy University of Colorado Denver ,
Boulder , CO , USA.; bfe University of California Irvine , Department of
Developmental and Cell Biology , Irvine , CA , USA.; bhx University of Cologne,
Medical Faculty, Center for Biochemistry , Cologne , Germany.; bke University of
Graz, Institute of Molecular Biosciences, BioTechMed Graz , Graz , Austria.; cbq
Weizmann Institute of Science , Department of Chemical Biology , Rehovot , Israel.;
bb Baylor College of Medicine , Department of Medicine , Houston , TX , USA.; ajz
Laboratory of Translational Oncology and Experimental Cancer Therapeutics,
Department of Hematology/Oncology and Molecular Therapeutics Program, Fox Chase
Cancer Center, Philadelphia, PA, USA.; aaf Luxembourg Institute of Health,
Department of Oncology, Luxembourg City , Luxembourg.; cbp Weizmann Institute of
Science , Department of Biological Chemistry , Rehovot , Israel.; arv Tel Aviv
University , Department of Human Molecular Genetics and Biochemistry , Sackler
School of Medicine , Tel Aviv , Israel.; byx University of York , Department of
Biology , Heslington, York , UK.; brj University of Pavia , Department of Health
Sciences , Pavia , Italy.; bxf University of Toronto, Sunnybrook Research
Institute, Sunnybrook Health Sciences Centre , Toronto, Ontario , Canada.; bqs
University of Oslo, Oslo University Hospital, Centre for Molecular Medicine Norway,
Nordic EMBL Partnership , Oslo , Norway.; aqr Stellenbosch University , Department
of Physiological Sciences , Stellenbosch , South Africa.; arp Technion-Israel
Institute of Technology, The Rappaport Faculty of Medicine and Research Institute ,
Department of Biochemistry , Haifa , Israel.; ajf Oslo University Hospital,
Institute for Microbiology , Oslo , Norway.; alu Ruhr University Bochum, Medical
Faculty, System Biochemistry , Bochum , Germany.; zk Latvian Biomedical Research
and Study Centre , Riga , Latvia.; bvn University of Tasmania, School of Health
Sciences , Launceston , Tasmania.; bkp University of Houston, College of Pharmacy,
Pharmacological and Pharmaceutical Sciences , Houston , TX , USA.; bmi University
of Ljubljana, Institute of Cell Biology, Faculty of Medicine , Ljubljana ,
Slovenia.; co C.S.I.C./U.A.M., Instituto de Investigaciones Biomédicas Alberto Sols
, Madrid , Spain.; bkj University of Helsinki , Department of Biosciences ,
Helsinki , Finland.; hb CNRS UM, Centre d'études d'agents Pathogènes et
Biotechnologies pour la Santé , Montpellier , France.; ec Centro de Investigaciones
Biológicas (CSIC) , Department of Cellular and Molecular Biology , Madrid , Spain.;
bka University of Glasgow, Institute of Infection, Immunity and Inflammation ,
Glasgow , UK.; bhu University of Cologne , Department of Dermatology , Cologne ,
Germany.; apx Spanish Council for Scientific Research, Institute for Advanced
Chemistry of Catalonia , Department of Biomedicinal Chemistry , Barcelona , Spain.;
amy Sapienza University of Rome , DAHFMO-Section of Anatomy , Rome , Italy.; um
IRCCS, Istituto Dermopatico dell'Immacolata , Rome , Italy.; bvc University of
Southern Denmark, Villum Center for Bioanalytical Sciences , Department of
Biochemistry and Molecular Biology , Odense , Denmark.; anb Sapienza University of
Rome , Department of Experimental Medicine , Rome , Italy.; zc Olivia Newton-John
Cancer Research Institute , Melbourne , Victoria , Australia.; ze La Trobe
University , Department of Chemistry and Physics , Melbourne , Victoria ,
Australia.; zf La Trobe University, School of Cancer Medicine , Melbourne ,
Victoria , Australia.; fk Chinese Academy of Sciences, Division of Physical Biology
and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute
of Applied Physics , Shanghai , China.; bui University of South Carolina School of
Medicine , Department of Cell Biology and Anatomy , Columbia , SC , USA.; bsc
University of Pittsburgh , Department of Surgery , Pittsburgh , PA , USA.; bne
University of Maryland, School of Medicine, Center for Biomedical Engineering and
Technology , Department of Physiology , Baltimore , MD , USA.; xu King's College
London , Department of Basic and Clinical Neuroscience , IoPPN , London , UK.; bdz
University of Brescia , Department of Molecular and Translational Medicine ,
Brescia , Italy.; iq Danish Cancer Society Research Center, Cell Death and
Metabolism Unit, Center for Autophagy, Recycling and Disease , Copenhagen ,
Denmark.; ayw Université de Lyon, INSERM, U 1111, Centre International de Recherche
en Infectiologie (CIRI), Ecole Normale Supérieure de Lyon, CNRS, UMR 5308 , Lyon ,
France.; rs INRA, UMR866 Dynamique Musculaire et Métabolisme , Montpellier ,
France.; aza Université de Montpellier , Montpellier , France.; agq National
University of Ireland, Pharmacology and Therapeutics , Galway , Ireland.; btk
University of Rome "Tor Vergata" , Department of Systems Medicine , Rome , Italy.;
ads Nanchang University, Institute of Life Science , Nanchang , China.; afl
National Institutes of Health, Experimental Transplantation and Immunology Branch,
National Cancer Institute , Bethesda , MD , USA.; atl Third Military Medical
University , Department of Neurosurgery , Southwest Hospital , Shapingba District,
Chongqing , China.; bkn University of Hong Kong , Hong Kong , China.; boc
University of Michigan , Department of Molecular , Cellular, and Developmental
Biology , Ann Arbor , MI , USA.; bog University of Michigan, Life Sciences
Institute , Ann Arbor , MI , USA.; cay Washington University in St. Louis, School
of Medicine , Department of Ophthalmology and Visual Sciences , St. Louis , MO ,
USA.; awu Universidad de Oviedo, Instituto Universitario de Oncología ,
Departamento de Bioquímica y Biología Molecular , Oviedo , Spain.; aws Universidad
de Navarra, Centro de Investigacion Medica Aplicada , Pamplona , Spain.; tr
Instituto de Investigaciones Biomedicas de Barcelona, CSIC-IDIBAPS and Centro de
Investigacion en Red en enfermedades hepáticas y digestivas, CIBEREHD, ISCIII ,
Barcelona , Spain.; bva University of Southern California, Research ALPD and
Cirrhosis Center, Keck School of Medicine , Los Angeles , CA , USA.; awr
Universidad de León, Área de Biología Celular, Instituto de Biomedicina , León ,
Spain.; abh Mayo Clinic, Schulze Center for Novel Therapeutics, Division of
Oncology Research , Department of Oncology , Rochester , MN , USA.; ayi Université
Catholique de Louvain (UCL), Institut de Recherche Expérimentale et Clinique (IREC)
, Brussels , Belgium.; uh IRCCS San Raffaele Pisana, Laboratory of Skeletal Muscle
Development and Metabolism , Rome , Italy.; bgw University of Campinas , Department
of Biochemistry and Tissue Biology , Campinas, São Paulo , Brazil.; biq University
of Debrecen , Debrecen , Hungary.; amn San Diego State University , Department of
Biology , San Diego , CA , USA.; abe Mayo Clinic , Department of Neuroscience ,
Jacksonville , FL , USA.; axc Universidad Federal do Rio Grande do Sul (UFRGS) ,
Department of Biophysics and Center of Biotechnology , Porto Alegre , Brazil.; ir
Danish Cancer Society Research Center, Cell Stress and Survival Unit , Copenhagen ,
Denmark.; btd University of Rome "Tor Vergata" , Department of Biology , Rome ,
Italy.; aez National Institute for Infectious Diseases "L. Spallanzani" IRCCS ,
Rome , Italy.; bto University of Salento , Department of Biological and
Environmental Sciences and Technologies (DiSTeBA) , Lecce , Italy.; aqs Stephen A.
Wynn Institute for Vision Research , Iowa City , IA , USA.; blg University of
Iowa , Department of Ophthalmology and Visual Sciences , Iowa City , IA , USA.; bgh
University of California San Francisco, Departments of Neurology and Physiology ;
Gladstone Institute of Neurological Disease , San Francisco , CA , USA.; afp
National Institutes of Health, National Heart, Lung, and Blood Institute , Bethesda
, MD , USA.; ss
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Department of Chemistry,
Portici (Naples), Italy.; bpg University of Na les Federico II , Department of
Veterinary Medicine and Animal Production , Naples , Italy.; cab Virginia
Commonwealth University, Institute of Molecular Medicine, Massey Cancer Center,
Virginia Commonwealth University, School of Medicine , Department of Human and
Molecular Genetics , Richmond , VA , USA.; aln Rockefeller University , New York ,
NY , USA.; bdv University of Bologna , Dipartimento di Scienze Biomediche e
Neuromotorie , Bologna , Italy.; az Babraham Institute, Signalling Program ,
Cambridge , UK.; bpg University of Naples Federico II , Department of Veterinary
Medicine and Animal Production , Naples , Italy.; bgn University of Cambridge,
Cambridge Institute for Medical Research , Cambridge , UK.; ll Fondazione IRCCS
Istituto Nazionale dei Tumori , Department of Experimental Oncology and Molecular
Medicine , Milan , Italy.; axs Università del Piemonte Orientale "A. Avogadro" ,
Dipartimento di Scienze della Salute , Novara , Italy.; abs McGill University,
McGill Parkinson Program , Department of Neurology and Neurosurgery , Montreal,
QC , Canada.; ug IRCCS Neuromed , Pozzilli, IS , Italy.; brt University of Pisa ,
Department of Translational Research and New Technologies in Medicine and Surgery ,
Pisa , Italy.; bal University Clinic Heidelberg , Department of Experimental
Surgery , Heidelberg , Germany.; asa Telethon Institute of Genetics and Medicine
(TIGEM) , Pozzuoli, Naples , Italy.; bpj University of Nebraska-Lincoln, Redox
Biology Center and School of Veterinary Medicine and Biomedical Sciences ,
Lincoln , NE , USA.; do Centre de Recherche du CHU de Québec, Faculty of Pharmacy ,
Québec , Canada.; azm Université Laval, Neurosciences Axis , Québec , Canada.; sq
Institut de Cancérologie de Lorraine , Vandoeuvre-Lès-Nancy Cedex , France.; bij
University of Copenhagen, Biotech Research and Innovative Center (BRIC) ,
Copenhagen , Denmark.; aft National Institutes of Health, NIAID, Laboratory of
Systems Biology , Bethesda , MD , USA.; blq University of Kiel , Department of
Cardiology , Kiel , Germany.; ayv Université de Lyon, Faculty of Medicine , Saint
Etienne , France.; adh MRC Cancer Unit, University of Cambridge, Hutchison/MRC
Research Centre , Cambridge , UK.; adg Icahn School of Medicine at Mount Sinai,
Division of Liver Diseases , New York , NY , USA.; qf Houston Methodist Research
Institute, Genomic Medicine Program , Houston , TX , USA.; bkq University of
Houston , Department of Biology and Biochemistry , Center for Nuclear Receptors and
Cell Signaling , Houston , TX , USA.; ala Queen's University of Belfast, Centre for
Experimental Medicine , Belfast , UK.; awq Universidad de Extremadura, Centro de
Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED) ,
Departamento de Bioquímica y Biología Molecular y Genética , Facultad de Enfermería
y Terapia Ocupacional , Cáceres , Spain.; bwd University of Texas, MD Anderson
Cancer Center , Department of Neuro-Oncology , Houston , TX , USA.; wn Juntendo
University, Graduate School of Medicine , Department of Metabolism and
Endocrinology , Tokyo , Japan.; afe National Institute of Neuroscience, National
Center of Neurology and Psychiatry , Department of Degenerative Neurological
Diseases , Tokyo , Japan.; as Asahikawa Medical University, Division of
Gastroenterology and Hematology/Oncology , Department of Medicine , Hokkaido ,
Japan.; aty Tohoku University , Department of Developmental Biology and
Neurosciences , Graduate School of Life Sciences, Sendai , Miyagi , Japan.; ns
Goethe University, Institute for Experimental Cancer Research in Pediatrics ,
Frankfurt , Germany.; uf IRCCS Casa Sollievo della Sofferenza, Medical Genetics
Unit , San Giovanni Rotondo (FG) , Italy.; acg Medical University of Silesia ,
Department of Pharmacology , Katowice , Poland.; oq Hannover Medical School ,
Department of Biochemistry , Hannover , Germany.; ayl Université Catholique de
Louvain (UCL), Laboratory of Cell Physiology , Brussels , Belgium.; cau Warsaw
University of Life Sciences (SGGW) , Department of Physiological Sciences , Faculty
of Veterinary Medicine , Warsaw , Poland.; u Al Jalila Foundation Research Centre ,
Dubai , UAE.; avi UAE University, Cell Signaling Laboratory , Department of
Biochemistry , College of Medicine and Health Sciences , Al Ain, Abu Dhabi , UAE.;
ath The Weizmann Institute of Science , Department of Plant Sciences , Rehovot ,
Israel.; tx Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
(INIA) , Departamento de Biotecnología , Madrid , Spain.; hr Complejo Hospitalario
Universitario de Albacete, Unidad de Neuropsicofarmacología , Albacete , Spain.;
bbn University Medical Center Hamburg-Eppendorf, Institute of Neuropathology ,
Hamburg , Germany.; km Centre de Recherche des Cordeliers, Equipe 11 labellisée par
la Ligue Nationale contre le Cancer , Paris , France.; oc Gustave Roussy Cancer
Campus , Villejuif , France.; sn INSERM, U1138 , Paris , France.; azv Université
Paris Descartes/Paris V , Paris , France.; bxz University of Urbino "Carlo Bo" ,
Department of Biomolecular Sciences , Urbino , Italy.; app Sorbonne Universités,
CNRS, UPMC, Univ Paris 06, UMR 7622, IBPS , Paris , France.; bja University of
Edinburgh, Edinburgh Cancer Research Centre , Edinburgh , UK.; qr Icahn School of
Medicine at Mount Sinai , Departments of Neurology and Psychiatry , Center for
Cognitive Health, Mount Sinai Alzheimer's Disease Research Center , New York , NY ,
USA.; vj James J. Peters VA Medical Center , Bronx , NY , USA.; bfh University of
California Irvine , Irvine , CA , USA.; bbx University of Adelaide, Alzheimer's
Disease Genetics Laboratory , Adelaide , Australia.; biv University of Dundee, MRC
Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences , Dundee ,
UK.; bjv University of Geneva, School of Medicine , Department of Pathology and
Immunology , Geneva , Switzerland.; bnn University of Massachusetts, Medical School
, Department of Neurology , Worcester , MA , USA.; ass The Fourth Military Medical
University, School of Basic Medical Sciences , Department of Physiology , Xi'an ,
China.; aoq Shanghai Jiao Tong University, State Key Laboratory of Oncogenes and
Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital,
School of Medicine , Shanghai , China.; bhf University of Chile, Advanced Center
for Chronic Diseases (ACCDiS), Division of Cardiovascular Diseases, Faculty of
Medicine , Santiago , Chile.; tn Instituto de Biología Molecular y Celular de
Rosario (IBR-CONICET) , Rosario , Argentina.; ae Albert Einstein College of
Medicine, Departments of Medicine (Endocrinology) and Molecular Pharmacology ,
Bronx , NY , USA.; tv Instituto de Investigaciones Biomédicas de Barcelona IIBB-
CSIC , Liver Unit, Hospital Clinic de Barcelona-IDIBAPS and CIBEREHD, Barcelona ,
Spain.; bmb University of Leuven, Campus Gasthuisberg , Department of Cellular and
Molecular Medicine , Laboratory for Cell Death Research and Therapy , Leuven ,
Belgium.; ak All India Institute of Medical Sciences , Department of
Gastroenterology , New Delhi , India.; awb Universidad Autónoma de Madrid, Centro
Nacional de Biotecnología (CNB-CSIC), Centro de Biología Molecular Severo Ochoa,
Departamento de Biología Molecular , Madrid , Spain.; abc Max Planck Institute of
Psychiatry, Translational Research in Psychiatry , Munich , Germany.; boc
Institute , Ann Arbor , MI , USA.; p Aix-Marseille Université, U2M, Centre
d'Immunologie de Marseille-Luminy , Marseille , France.; hd CNRS, UMR 7280 ,
Marseille , France.; sl INSERM, U1104 , Marseille , France.; dq Centre de Recherche
en Cancérologie de Nantes-Angers, CNRS UMR6299, INSERM U892 , Nantes , France.; ad
Albert Einstein College of Medicine, Departments of Biochemistry and of Medicine ,
Bronx , NY , USA.; bex University of California Berkeley, Howard Hughes Medical
Institute , Department of Molecular and Cell Biology , Berkeley , CA , USA.; lf
First Hospital of Jilin University , Department of Neurosurg ry , Changchun ,
China.; aoj Shanghai Jiao Tong University, School of Medicine , Department of
Biochemistry and Molecular Biology , Shanghai , China.; aem National Cheng Kung
University , Department of Pharmacology , Tainan , Taiwan.; if CSS-Mendel
Institute, Neurogenetics Unit , Rome , Italy.; axt Università del Piemonte
Orientale , Novara , Italy.; ox Harvard Medical School , Boston , MA , USA.; du
Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des
Plantes, Unité Propre de Recherche , Strasbourg , France.; bqs University of Oslo,
Oslo University Hospital, Centre for Molecular Medicine Norway, Nordic EMBL
Partnership , Oslo , Norway.; bgf University of California San Francisco ,
Department of Pharmaceutical Chemistry , San Francisco , CA , USA.; caf Virginia
Commonwealth University, Massey Cancer Center , Richmond , VA , USA.; bmz
University of Manitoba , Department of Human Anatomy and Cell Science , Winnipeg,
Manitoba , Canada.; o Aix-Marseille Université, CNRS UMR 7278, IRD198, INSERM
U1095, Medicine Faculty , Marseille , France.; al All India Institute of Medical
Sciences , Department of Physiology , New Delhi , India.; vd Istituto Superiore di
Sanità , Rome , Italy.; amp San Raffaele Institute, Dept. of Therapeutic Research
and Medicine Evaluation , Sulmona, L'Aquila , Italy.; akh Polytechnic University of
Marche , Department of Clinical Science , Faculty of Medicine , Ancona , Italy.;
amx Sapienza University of Rome, DAHFMO-Section of Histology , Rome , Italy.; iv
Democritus University of Thrace , Department of Pathology , Alexandroupolis ,
Greece.;
bqw University of Ottawa , Department of Cellular and Molecular Medicine , Ottawa,
Ontario , Canada.; box University of Modena and Reggio Emilia , Department of
Surgery , Medicine, Dentistry and Morphological Sciences , Modena , Italy.; bmy
University of Manitoba, CancerCare Manitoba, Manitoba Institute of Cell Biology ,
Departments of Biochemistry and Medical Genetics and Immunology , Winnipeg,
Manitoba , Canada.; blw University of Lausanne , Department of Fundamental
Neurosciences , Faculty of Biology and Medicine , Lausanne , Switzerland.; asc
Temple University, Sbarro Institute for Cancer Research and Molecular Medicine,
Center for Biotechnology, College of Science and Technology , Philadelphia , PA ,
USA.; btb University of Siena, Department of Medicine, Surgery & Neuroscience,
Siena, Italy.; bma University of Leicester , Department of Genetics , Leicester ,
UK.; ux Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa , Pisa ,
Italy.; cak VU University Medical Center , Department of Medical Oncology ,
Amsterdam , The Netherlands.; bxa University of Toronto , Department of Laboratory
Medicine and Pathobiology , Toronto, Ontario , Canada.; no Goethe University
Medical School, Experimental Neurology , Frankfurt am Main , Germany.; rw Centre
Scientifique de Monaco, Biomedical Department, Monaco, Principality of Monaco.; rx
University of Nice Sophia Antipolis, Institute of Research on Cancer and Ageing of
Nice, CNRS UMR 7284/INSERM U1081 , Nice , France.; gx Cleveland Clinic , Department
of Cancer Biology , Cleveland , OH , USA.; awm Universidad de Chile, Facultad de
Ciencias , Departamento de Biología , Centro de Regulación del Genoma , Santiago ,
Chile.; bej University of British Columbia , Department of Urological Sciences ,
Vancouver, BC , Canada.; bpb University of Montpellier, UMR 5554 , Montpellier ,
France.; bjw University of Georgia, College of Veterinary Medicine , Department of
Biosciences and Diagnostic Imaging , Athens , GA , USA.; bzh US Food and Drug
Administration, National Center for Toxicology Research, Division of Microbiology ,
Jefferson , AR , USA.; axi Faculdade de Ciências Farmacêuticas de Ribeirão Preto,
Universidade de São Paulo (FCFRP, USP) , São Paulo , Brazil.; afa National
Institute for Infectious Diseases , Department of Epidemiology and Preclinical
Research , Translational Research Unit , Rome , Italy.; ahi New York Medical
College , Department of Medicine, Pharmacology, and Physiolog , Valhalla , NY ,
USA.; bfb University of California Davis , Department of Neurobiology , Physiology,
and Behavior , Davis , CA , USA.; it Danish Cancer Society Research Center, Unit of
Cell Stress and Survival , Copenhagen , Denmark.; bry University of Pittsburgh ,
Department of Critical Care Medicine , Center for Critical Care Nephrology,
Clinical Research Investigation and Systems Modeling of Acute Illness (CRISMA)
Center , Pittsburgh , PA , USA.; bwd University of Texas, MD Anderson Cancer Center
, Department of Neuro-Oncology , Houston , TX , USA.; awq Universidad de
Extremadura, Centro de Investigación Biomédica en Red sobre Enfermedades
Neurodegenerativas (CIBERNED) , Departamento de Bioquímica y Biología Molecular y
Genética , Facultad de Enfermería y Terapia Ocupacional , Cáceres , Spain.; bts
University of São Paulo, Ribeirão Preto Medical School , Department of Biochemistry
and Immunology , Ribeirão Preto, São Paulo , Brazil.; jx Ege University, Faculty of
Science , Department of Biology , Bornova , Izmir , Turkey.; ady Nankai University,
College of Life Sciences , Tianjin , China.; ur IRCM, INSERM, U896, Institut de
Recherche en Cancérologie de Montpellier , Montpellier , France.; avz Universidad
Austral de Chile , Department of Physiology , Valdivia , Chile.; brm University of
Pennsylvania, Center for Cell and Molecular Therapy, The Children Hospital of
Philadelphia , Department of Neurology , Perelman School of Medicine , Philadelphia
, PA , USA.; go CIBER de Enfermedades Raras (CIBERER) , Valencia , Spain.; akm
Program in Rare and Genetic Diseases, Centro de Investigación Príncipe Felipe
(CIPF), IBV/CSIC Associated Unit at CIPF , Valencia , Spain.; awq Universidad de
Genética , Facultad de Enfermería y Terapia Ocupacional , Cáceres , Spain.; bcf
University of Alberta , Department of Biochemistry , Edmonton, Alberta , Canada.;
bya University of Utah School of Medicine , Department of Biochemistry , Salt Lake
City , UT , USA.; avw Uniformed Services University of the Health Sciences ,
Department of Anesthesiology , Bethesda , MD , USA.; bwz University of Toronto ,
Department of Cell and Systems Biology , Toronto, Ontario , Canada.; agp National
University of Ireland, Apoptosis Research Centre , Galway , Ireland.; ci British
Columbia Cancer Agency , Genome Sciences Centre , Vancouver, BC , Canada.; apf
Simon Fraser University, Department of Molecular Biology and Biochemistry ,
Burnaby, BC , Canada.; aat Massachusetts General Hospital and Harvard Medical
School, Cutaneous Biology Research Center , Charlestown , MA.; yw Kyoto
University , Department of Botany , Kyoto , Japan.; hx Consejo Superior de
Investigaciones Científicas (CSIC), Instituto de Bioquímica Vegetal y
Fotosíntesis , Sevilla , Spain.; dd Cedars-Sinai Heart Institute, Barbra Streisand
Women's Heart Center , Los Angeles , CA , USA.; arz Tel Aviv University, Sackler
Faculty of Medicine , Tel Aviv , Israel.; amh Sabanci University, Molecular
Biology, Genetics and Bioengineering Program , Istanbul , Turkey.; n Aix Marseille
Université, CNRS, IBDM, UMR 7288 , Campus de Luminy, Marseille , France.; aay Max
Planck Institute for Biology of Ageing , Cologne , Germany.; asc Temple University,
Sbarro Institute for Cancer Research and Molecular Medicine, Center for
Biotechnology, College of Science and Technology , Philadelphia , PA , USA.; nx
Griffith University, Menzies Health Institute Queensland , Australia.; bzz Virginia
Commonwealth University , Department of Internal Medicine , Richmond , VA , USA.;
blu University of L'Aquila , Department of Biotechnological and Applied Clinical
Sciences , Division of Radiotherapy and Radiobiology , L'Aquila , Italy.; aqe St.
Jude Children's Research Hospital , Memphis , TN , USA.; beb University of Bristol,
School of Cellular and Molecular Medicine , Bristol , UK.; alq Royal Military
College, Chemistry and Chemical Engineering, Kingston , N , Canada.; uw Istituto
Italiano di Tecnologia , Department of Drug Discovery and Development , Laboratory
of Molecular Medicine , Genoa , Italy.; azj Université de Strasbourg/CNRS UPR3572,
Immunopathologie et Chimie Thérapeutique, IBMC , Strasbourg , France.; blc
University of Iowa, Children's Hospital , Iowa City , IA , USA.; ams Sanford
Burnham Medical Research Institute, Cell Death and Survival Networks Program , La
Jolla , CA , USA.; acm Medical University of Vienna , Department of Dermatology ,
CD Lab - Skin Aging , Vienna , Austria.; nq Goethe University School of Medicine,
Institute of Biochemistry II and Buchmann Institute for Molecular Life Sciences ,
Frankfurt am Main , Germany.; brc University of Padova , Department of Molecular
Medicine , Padova , Italy.; nl German Institute of Human Nutrition , Department of
Molecular Toxicology , Nuthetal , Germany.; bhl University of Cincinnati College of
Medicine , Department of Cancer Biology , Cincinnati , OH , USA.; bfr University of
California San Diego , Department of Pharmacology and Moores Cancer Center , La
Jolla , CA , USA.; bvc University of Southern Denmark, Villum Center for
Bioanalytical Sciences , Department of Biochemistry and Molecular Biology ,
Odense , Denmark.; awe Universidad Complutense, School of Pharmacy , Madrid ,
Spain; and CIBER de Diabetes y Enfermedades Metab olicas Asociadas (CIBERDEM),
Instituto de Salud Carlos III, Madrid, Spain.; gy Cleveland Clinic , Department of
Cellular and Molecular Medicine , Cleveland , OH , USA.; yj KU Leuven , Clinical
Division and Laboratory of Intensive Care Medicine , Department Cellular and
Molecular Medicine , Leuven , Belgium.; aus Tongji University School of Medicine ,
Department of Gastroenterology , Shanghai Tenth People's Hospital , Shanghai ,
China.; lm Food and Drug Administration (FDA), Division of Biochemical Toxicology,
National Center for Toxicological Research (NCTR) , Jefferson , AR , USA.; avp UCLA
David Geffen School of Medicine, Brain Research Institute , Los Angeles , CA ,
USA.; vr Jiangsu University, School of Pharmacy , Zhenjiang, Jiangsu , China.; atf
The Third Affiliated Hospital of Guangzhou Medical University , Department of
Clinical Laboratory Medicine , Guangzhou, Guangdong , China.; bxn University of
Tübingen, Center for Plant Molecular Biology (ZMBP) , Department of Plant
Biochemistry , Tübingen , Germany.; bga University of California San Diego, Skaggs
School of Pharmacy and Pharmaceutical Sciences , La Jolla , CA , USA.; bvi
University of Sydney , Department of Pathology and Bosch Institute , Sydney, New
South Wales , Australia.; lt The Francis Crick Institute, Mill Hill Laboratory ,
London , UK.; nv Graduate School of Cancer Science and Policy , Department of
System Cancer Science , Goyang , Korea.; bdx University of Bonn, Institute for Cell
Biology , Bonn , Germany.; cd Brandeis University , Department of Biology , Waltham
, MA , USA.; auf Tokai University School of Medicine , Department of Molecular Life
Sciences , Kanagawa , Japan.; bp Bernhard Nocht Institute for Tropical Medicine ,
Hamburg , Germany.; gr Cincinnati Children's Hospital Medical Center, Division of
Clinical Pharmacology , Cincinnati , OH , USA.; bna University of Manitoba ,
Department of Physiology and Pathophysiology , Winnipeg, Manitoba , Canada.; ni
German Cancer Research Center (DKFZ),
Lysosomal Systems Biology , Heidelberg , Germany.; alj RIKEN Brain Science
Institute, Laboratory for Developmental Neurobiology , Saitama , Japan.; azw
Université Paris Descartes-Sorbonne Paris Cité, Institut Necker Enfants-Malades
(INEM), INSERM U1151-CNRS UMR 8253 , Paris , France.; nt Goethe University,
Institute for Molecular Biosciences, Molecular Developmental Biology , Frankfurt ,
Hesse , Germany.; aix Osaka University , Department of Genetics , Graduate School
of Medicine , Osaka , Japan.; bpf University of Namur, Research Unit in Molecular
Physiology (URPhyM) , Namur , Belgium.; aor University of Sharjah, College of
Medicine, United Arab Emirates.; bqz University of Oxford, CRUK/MRC Oxford
Institute for Radiat on Oncology , Oxford , UK.; ccx Zhejiang University, Institute
of Pharmacology, Toxicology and Biochemical Pharmaceutics , Hangzhou , China.; cda
Zhejiang University, Sir Run Run Shaw Hospital, College of Medicine , Hangzhou,
Zhejiang , China.; wk Johns Hopkins, School of Medicine, Wilmer Eye Institute ,
Baltimore , MD , USA.; afw National Institutes of Health, NIDDK, LCMB , Bethesda ,
MD , USA.; amt Sanford Burnham Prebys Medical Discovery Institute , La Jolla , CA ,
USA.; bqg University of Occupational and Environmental Health , Third Department of
Internal Medicine , Kitakyushu , Japan.; bdj University of Belgrade, Institute for
Biological Research "Sinisa Stankovic" , Belgrade , Serbia.; pb Harvard Medical
School , Department of Cell Biology , Boston , MA , USA.; xs King Saud University,
College of Science , Department of Zoology , Riyadh , Saudi Arabia.; ajk Oxford
University, Department of Oncology , Weatherall Institute of Molecular Medicine,
John Radcliffe Hospital, Molecular Oncology Laboratories , Oxford , UK.; acw Monash
University, Centre for Inflammatory Diseases, Lupus Research Laboratory , Clayton ,
Victoria , Australia.; bju University of Geneva , Department of Cellular Physiology
and Metabolism , Geneva , Switzerland.; bay University Hospital Freiburg ,
Department of Medicine II , Freiburg , Germany.; wt Kagoshima University, Graduate
School of Medical and Dental Sciences, Division of Human Pathology , Department of
Oncology , Course of Advanced Therapeutics , Kagoshima , Japan.; mt George
Washington University , Department of Anatomy and Regenerative Biology ,
Washington, DC , USA.; mu George Washington University, Flow Cytometry Core
Facility , Washington, DC , USA.; aia Northwestern University , Department of Cell
and Molecular Biology , Feinberg School of Medicine , Chicago , IL , USA.; ags
National University of Singapore , Department of Biological Sciences , Singapore.;
atk Third Military Medical University , Department of Biochemistry and Molecular
Biology , Chongqing , China.; apd Sichuan University, West China Hospital, State
Key Labortary of Biotherapy , Sichuan , China.; vv Jinan University, Anti-stress
and Health Center, College of Pharmacy , Guangzhou , China.; vw Jinan University ,
Department of Immunobiology , College of Life Science and Technology , Guangzhou ,
China.; ji Duke University, Medical Center , Department of Immunology , Durham , NC
, USA.; bhb University of Chicago , Department of Medicine , Section of Dermatology
, Chicago , IL , USA.; atg The Walter and Eliza Hall Institute of Medical Research,
Development and Cancer Division , Parkville , VIC , Australia.; azb Université de
Montréal , Department of Medicine , Montréal, Quebec , Canada.; afx National
Institutes of Health, Rocky Mountain Laboratories, NIAID, Coxiella Pathogenesis
Section , Hamilton , MT , USA.; bkb University of Glasgow, Wolfson Wohl Cancer
Research Centre, MVLS, Institute of Cancer Sciences , Glasgow , UK.; bqt University
of Osnabrueck, Division of Microbiology , Osnabrueck , Germany.; cg Brigham and
Women's Hospital, Harvard Medical School , Boston , MA , USA.; caw Washington State
University, School of Molecular Biosciences , Pullman , WA , USA.; aij Ohio State
University , Department of Molecular Genetics , Columbus , OH , USA.; axj
Universidade de São Paulo , Departamento de Parasitología , Instituto de Ciências
Biomédicas , São Paulo , Brazil.; lg FISABIO, Hospital Dr. Peset , Valencia ,
Spain.; hs Complutense University, Instituto de Investigaciones Sanitarias San
Carlos (IdISSC) , Department of Biochemistry and Molecular Biology I , School of
Biology , Madrid , Spain.; bge FONDAP Center for Geroscience, Brain Health and
Metabolism, Santiago, Chile.; bhj University of Chile, Institute of Biomedical
Sciences, Center for Molecular Studies of the Cell, Program of Cellular, Molecular
Biology and Biomedical Neuroscience Institute, Faculty of Medicine , Santiago ,
Chile.; bwn University of Texas, Southwestern Medical Center , Dallas , TX , USA.;
auv Tottori University, Research Center for Bioscience nd Technology , Yonago ,
Japan.; hw Consejo Superior de Investigaciones Científicas (CSIC), Institute of
Parasitology and Biomedicine López-Neyra , Granada , Spain.; bmn University of
Louisville , Department of Medicine , Institute of Molecular Cardiology, Diabetes
and Obesity Center , Louisville , KY , USA.; bwr University of Texas, Southwestern
Medical Center, Medicine and Molecular Biology , Dallas , TX.; nb Georgia Regents
University , Department of Neurology , Augusta , GA , USA.; nc Georgia Regents
University , Department of Orthopaedic Surgery , Augusta , GA , USA.; nd Georgia
Regents University, Institute for Regenerative and Reparative Medicine , Augusta ,
GA , USA.; nf Georgia Regents University, Medical College of Georgia , Department
of Cellular Biology and Anatomy , Augusta , GA , USA.; xm Kawasaki Medical School ,
Department of Hepatology and Pancreatology , Kurashiki, Okayama , Japan.; arh
Swedish University of Agricultural Sciences and Linnean Center for Plant Biology ,
Department of Plant Biology , Uppsala BioCenter , Uppsala , Sweden.; bpt University
of Nice-Sophia Antipolis, INSERM U1081, CNRS 7284, Faculty of Medicine , Nice ,
France.; nk German Center for Neurodegenerative Diseases (DZNE) , Munich ,
Germany.; ars Technische Universität München , Department of Neurology , Munich ,
Germany.; bdx University of Bonn, Institute for Cell Biology , Bonn , Germany.; w
Albert Einstein Cancer Center , New York , NY , USA.; ccg Yeshiva University , New
York , NY , USA.; rp Inje University , Department of Rehabilitation Science,
College of Biomedical Science & Engineering, u-Healthcare & Anti-aging Research
Center (u-HARC), Gimhae , Korea.; cco York University, School of Kinesiology and
Health Science , Toronto, Ontario , Canada.; cam VU University Medical Center ,
Department of Pathology , Amsterdam , The Netherlands.; bhv University of Cologne,
Institute for Genetics, CECAD Research Center , Cologne , Germany.; blk University
of Kaohsiung Medical University , Department of Physiology , Faculty of Medicine,
College of Medicine , Kaohsiung , Taiwan.; ek Chang Gung University , Department of
Biomedical Sciences , College of Medicine , Taoyuan , Taiwan.; agk National Taiwan
University, Institute of Molecular Medicine, College of Medicine , Taipei ,
Taiwan.; ao Anhui University of Science and Technology , Department of Immunology
and Medical Inspection , Huainan , Anhui , China.; bku University of Illinois at
Chicago, Departments of Anesthesiology and Pharmacology , Chicago , IL , USA.; akn
Providence Portland Medical Center, Earle A. Chiles Research Institute , Portland ,
OR , USA.; fa China Agricultural University , Department of Nutrition and Food
Safety , Beijing , China.; bwo University of Texas, Southwestern Medical Center ,
Department of Internal Medicine , Center for Mineral Metabolism and Clinical
Research , Dallas , TX , USA.; agm National Tsing Hua University , Department of
Chemical Engineering , Hsinchu , Taiwan.; fg Chinese Academy of Medical Sciences
and Peking Union Medical College, Molecular Immunology and Cancer Pharmacology
Group, State Key Laboratory of Bioactive Substance and Function of Natural
Medicines, Institute of Materia Medica , Beijing , China.; fg Chinese Academy of
Medical Sciences and Peking Union Medical College, Molecular Immunology and Cancer
Pharmacology Group, State Key Laboratory of Bioactive Substance and Function of
Natural Medicines, Institute of Materia Medica , Beijing , China.; boi University
of Michigan, Neurosurgery , Ann Arbor , MI , USA.; apc Sichuan University, State
Key Laboratory of Biotherapy/Collaborative Innovation Center of Biotherapy; West
China Hospital , Chengdu , China.; em Chang Jung Christian University , Department
of Bioscience Technology , Tainan , Taiwan.; agi National Taiwan University ,
Department of Urology , College of Medicine , Taipei , Taiwan.; el Chang Gung
University, Molecular Regulation and Bioinformatics Laboratory , Department of
Parasitology , Taoyuan , Taiwan.; zy Louisiana State University Health Sciences
Center , Department of Bio hemistry and Molecular Biology , Shreveport , LA , USA.;
aoo Shanghai Jiao Tong University, School of Medicine, Shanghai Institute of
Immunology , Shanghai , China.; agg National Taiwan University , Department of Life
Science , Taipei , Taiwan.; aom Shanghai Jiao Tong University, School of Medicine,
Renji Hospital , Shanghai , China.; qp Icahn School of Medicine at Mount Sinai ,
Department of Neuroscience , New York , NY , USA.; v Albany Medical College, Center
for Neuropharmacology and Neuroscience , Albany , NY , USA.; ai Albert Ludwigs
University, Renal Division , Freiburg , Germany.; bx BIOSS Centre for Biological
Signalling Studies , Freiburg , Germany.; bbl University Medical Center Freiburg ,
Freiburg , Germany.; bls University of Kiel, Institute of Human Nutrition and Food
Science , Kiel , Germany.; ant Seoul National University , Department of Biological
Sciences , Seoul , Korea.; bkk University of Helsinki , Department of Physiology ,
Faculty of Medicine , Helsinki
, Finland.; blh University of Jyväskylä , Department of Biology of Physical
Activity , Jyväskylä , Finland.; gk Chungnam National University, School of
Medicine , Department of Pharmacology , Daejeon , Korea.; bew University of
California Berkeley , Department of Molecular and Cell Biology , Berkeley , CA ,
USA.; apz St. Anna Kinderkrebsforschung, Children's Cancer Research Institute,
Immunological Diagnostics , Vienna, Austria.; abk McGill University , Department of
Critical Care , Montreal, Quebec , Canada.; abp McGill University, Health Centre ,
Department of Medicine , Montreal, Quebec , Canada.; afr National Institutes of
Health, National Institute of Environmental Health Sciences, Clinical Research
Program , Research Triangle Park , NC , USA.; at Asan Medical Center, Asan
Institute for Life Sciences , Seoul , Korea.; bhc University of Chicago ,
Department of Pathology , Chicago , IL , USA.; aoy Shin Kong Wu Ho-Su Memorial
Hospital , Department of Urology , Taipei , Taiwan.; aur Tokyo Women's Medical
University , Department of Endocrinology and Hypertension , Tokyo , Japan.; wl
Juntendo University , Department of Research for Parkinson's Disease , Tokyo ,
Japan.; bow University of Modena and Reggio Emilia , Department of Life Sciences ,
Modena , Italy.; ar Asahi University, School of Dentistry , Department of Oral
Microbiology , Division of Oral Infections and Health Sciences , Mizuho , Gifu ,
Japan.; ar Asahi University, School of Dentistry , Department of Oral
Microbiology , Division of Oral Infections and Health Sciences , Mizuho , Gifu ,
Japan.; btp University of Salerno , Department of Pharmacy , Fisciano, Salerno ,
Italy.; dp Centre de Recherche en Cancérologie de Marseille (CRCM), INSERM U1068,
CNRS UMR 7258, Aix-Marseille Université , Institut Paoli-Calmette, Parc
Scientifique et Technologique de Luminy, Marseille , France.; atq Thomas Jefferson
University , Department of Pathology , Anatomy and Cell Biology , Philadelphia , PA
, USA.; py The Hong Kong University of Science and Technology, Clear Water Bay ,
Kowloon, Hong Kong.; pc Harvard Medical School, Laboratory of Comparative
Immunology, Center for the Study of Inflammatory Bowel Disease, Massachusetts
General Hospital Research Institute , Boston , MA , USA.; ed Centro de
Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Universidad
Nacional de Córdoba , Departamento de Bioquímica Clínica , Facultad de Ciencias
Químicas , Córdoba , Argentina.; aiu Osaka University Graduate School of Medicine ,
Department of Nephrology , Osaka , Japan.; baj University Belgrade, School of
Medicine , Belgrade , Serbia.; ev Children's Hospital of Philadelphia, Research
Institute , Philadelphia , PA , USA.; akd Perelman School of Medicine at the
University of Pennsylvania , Departments of Pediatrics and Systems Pharmacology and
Translational Therapeutics , Philadelphia , PA , USA.; bsn University of
Pittsburgh, Vascular Medicine Institute , Pittsburgh , PA , USA.; th Institute of
Microbial Technology (IMTECH), Cell Biology and Immunology Division , Chandigarh ,
India.; au Tohoku University, Graduate School of Agricultural Sciences , Sendai ,
Japan.; xp Keio University, Graduate School of Pharmaceutical Sciences , Department
of Biochemistry , Tokyo , Japan.; air Oregon State University , Department of
Pharmaceutical Sciences , College of Pharmacy , Corvallis , OR , USA.; axs
Università del Piemonte Orientale "A. Avogadro" , Dipartimento di Scienze della
Salute , Novara , Italy.; adq Nagoya University School of Medicine , Nagoya ,
Japan.; aru Technische Universität München, Plant Systems Biology , Freising ,
Germany.; bij University of Copenhagen, Biotech Research and Innovative Center
(BRIC) , Copenhagen , Denmark.; jm Duke-NUS Graduate Medical School, Cancer and
Stem Cell Biology Program , Singapore.; et Chiba University , Department of
Nanobiology , Chiba , Japan.; bzx Virginia Commonwealth University , Department of
Human and Molecular Genetics , Richmond , VA , USA.; on Hampton University ,
Department of Pharmaceutical Sciences , School of Pharmacy , Hampton , VA , USA.;
dy Centro de Biologia Molecular "Severo Ochoa" (UAM/CSIC), Consejo Superior de
Investigaciones Científicas, Universidad Autónoma de Madrid , Department of Cell
Biology and Immunology , Madrid , Spain.; amk Saitama Medical University, Saitama
Medical Center , Department of General Thoracic Surgery , Saitama , Japan.; km
Centre de Recherche des Cordeliers, Equipe 11 labellisée par la Ligue Nationale
contre le Cancer , Paris , France.; oc Gustave Roussy Cancer Campus , Villejuif ,
France.; sn INSERM, U1138 , Paris , France.; azv Université Paris Descartes/Paris V
, Paris , France.; iq Danish Cancer Society Research Center, Cell Death and
Metabolism Unit, Center for Autophagy, Recycling and Disease , Copenhagen ,
Denmark.; aqu Stony Brook University , Department of Molecular Genetics and
Microbiology , Stony Brook , NY , USA.; bkc University of Göttingen, Department of
Geobiology , Göttingen , Germany.; bni University of Maryland, School of Medicine ,
Department of Microbiology and Immunology , Baltimore , MD , USA.; aj All India
Institute of Medical Sciences , Department of Anatomy , New Delhi , India.; avl UCL
Institute of Child Health and Great Ormond Street Hospital for Children NHS
Foundation Trust , London , UK.; bvt University of Texas, Health Science Center at
Houston , Department of Pathology and Laboratory Medicine , Houston , TX , USA.;
aje Oslo University Hospital , Department of Molecular Cell Biology , Institute for
Cancer Research , Oslo , Norway.; bqk University of Oslo, Centre for Cancer
Biomedicine , Oslo , Norway.; aef National Brain Research Centre , Manesar, Gurgaon
, India.; xo Keimyung University, School of Medicine, Division of Gastroenterology
and Hepatology , Department of Internal Medicine , Daegu , Korea.; bib University
of Colorado, Denver ; and Denver VAMC , Denver , CO , USA.; aae Luxembourg
Institute of Health, Laboratory of Experimental Hemato-Oncology , Department of
Oncology , Luxembourg City , Luxembourg.; btu University of São Paulo, School of
Physical Education and Sport, Cellular and Molecular Exercise Physiology Laboratory
, São Paulo , Brazil.; bvi University of Sydney , Department of Pathology and Bosch
Institute , Sydney, New South Wales , Australia.; azh Université de Sherbrooke ,
Department of Anatomy and Cell Biology , Faculty of Medicine and Health Sciences ,
Sherbrooke, QC , Canada.; ep Charité - Universitätsmedizin Berlin , Department of
Neuropathology , Campus Charité Mitte , Berlin , Germany.; anp Seoul National
University College of Medicine , Department of Physiology and Biomedical Sciences ,
Seoul , Korea.; e Aarhus University , Department of Clinical Medicine , Aarhus ,
Denmark.; gi Chungbuk National University, College of Veterinary Medicine ,
Cheongju, Chungbuk , Korea.; li Florida Atlantic University , Department of
Biological Sciences , Jupiter , FL , USA.; mb Cancer Institute, Fudan University
Shanghai Cancer Center , Collaborative Innovation Center of Cancer Medicine,
Department of Oncology , Shanghai Medical College , Fudan University, Shanghai ,
China.; bwd University of Texas, MD Anderson Cancer Center , Department of Neuro-
Oncology , Houston , TX , USA.; asp The First Affiliated Hospital of Harbin Medical
University , Department of General Surgery , Harbin, Heilongjiang Province ,
China.; gd Chinese University of Hong Kong, School of Life Science, Centre for Cell
and Developmental Biology and State Key Laboratory of Agrobiotechnology , Sha Tin,
Hong Kong.; adu Nanjing Medical University , Department of Neurology , Nanjing
First Hospital , Nanjing , China.; bek University of British Columbia, Medical
Genetics, and BC Cancer Agency, Terry Fox Laboratory , Vancouver, BC , Canada.; fu
Chinese Academy of Sciences, State Key Laboratory of Mycology, Institute of
Microbiology , Beijing , China.; adb Mossakowski Medical Research Centre, Polish
Academy of Sciences, Electron Microscopy Platform , Warsaw , Poland.; dg Center for
Dementia Research, Nathan S. Kline Institute , Orangeburg , NY , USA.; ahn New York
University , Department of Psychiatry , New York , NY , USA.; lw Freshwater
Aquaculture Collaborative Innovation Center of Hubei Province , Wuhan , China.; qj
Huazhong Agricultural University , Department of Aquatic Animal Medicine , College
of Fisheries , Wuhan , China.; awv Universidad de Salamanca, Campus Miguel de
Unamuno , Departamento de Microbiología y Genética , Salamanca , Spain.; fu Chinese
Academy of Sciences, State Key Laboratory of Mycology, Institute of Microbiology ,
Beijing , China.; cct Zhejiang University , Hangzhou , China.; bpr University of
Newcastle, School of Medicine and Public Health , Callaghan, NSW , Australia.; boc
Institute , Ann Arbor , MI , USA.; amf Rutgers University-Robert Wood Johnson
Medical School , Pharmacology Department , Piscataway , NJ , USA.; but University
of South Florida , Department of Pharmaceutical Sciences , College of Pharmacy,
Byrd Alzheimer's Institute , Tampa , FL , USA.; gl Chungnam National University,
School of Medicine, Infection Signaling Network Research Center , Daejeon , Korea.;
bxm University of Tromsø - The Arctic University of Norway, Molecular Cancer
Research Group, Institute of Medical Biology , Tromsø , Norway.; brw University of
Pittsburgh Cancer Institute , Pittsburgh , PA , USA.; bsz University of Rochester
Medical Center , Department of Anesthesiology , Rochester , NY , USA.; bee
University of British Columbia , Department of Cellular and Physiological
Sciences , Vancouver, BC , Canada.; bwf University of Texas, MD Anderson Cancer
Center , Houston , TX , USA.; te Institute of Cancer Research, Divisions
of Molecular Pathology and Cancer Therapeutics , London , UK.; alc Radboud
University Nijmegen Medical Center , Department of Internal Medicine , Nijmegen ,
The Netherlands.; awh Universidad de Castilla-La Mancha, Facultad de Medicina ,
Departamento Ciencias Medicas , Albacete , Spain.; bjk University of Florida ,
Department of Aging and Geriatric Research , Gainesville , FL , USA.; xg Karolinska
Institute, Cancer Center Karolinska , Department of Oncology-Pathology ,
Stockholm , Sweden.; bst University of Pretoria , Department of Physiology ,
Pretoria, Gauteng , South Africa.; mc Fudan University , Department of Biosynthesis
, Key Laboratory of Smart Drug Delivery, Ministry of Education, School of
Pharmacy , Shanghai , China.; aqv Stony Brook University , Department of
Pathology , Stony Brook , NY , USA.; agf National Taiwan University , Department of
Life Science , Institute of Molecular and Cellular Biology , Taipei , Taiwan.; bci
University of Amsterdam , Department of Cellbiology and Histology , Academic
Medical Center , Amsterdam , The Netherlands.; kj Eötvös Loránd University ,
Department of Anatomy , Cell and Developmental Biology , Budapest , Hungary.; anr
Seoul National University Hospital , Department of Internal Medicine , Seoul ,
Korea.; bux University of Southern California, Keck School of Medicine , Department
of Molecular icrobiology and Immunology , Los Angeles , CA , USA.; ant Seoul
National University , Department of Biological Sciences , Seoul , Korea.; kx
Evelina's Children Hospital, Guy's and St. Thomas' Hospital NHS Foundation Trust ,
Department of Paediatric Neurology , Neuromuscular Service , London , UK.; xu
King's College London , Department of Basic and Clinical Neuroscience , IoPPN ,
London , UK.; xv King's College, Randall Division of Cell and Molecular Biophysics,
Muscle Signalling Section , London , UK.; ri Indiana University School of Medicine,
Biochemistry and Molecular Biology , Denver , CO , USA.; afy National Jewish Health
, Denver , CO , USA.; aag Maastricht University, Maastricht Radiation Oncology
(MaastRO) Lab, GROW - School for Oncology and Developmental Biology , Maastricht ,
The Netherlands.; bpp University of New South Wales, School of Biotechnology and
Biomolecular Sciences , Sydney, NSW , Australia.; biy University of Eastern
Finland, Kuopio University Hospital , Department of Ophthalmology , Kuopio ,
Finland.; bvm University of Tartu, Institute of Biomedicine and Translational
Medicine , Tartu , Estonia.; afe National Institute of Neuroscience, National
Center of Neurology and Psychiatry , Department of Degenerative Neurological
Diseases , Tokyo , Japan.; avt UMR 1280 , Nantes , France.; zo Linköping University
, Department of Clinical and Experimental Medicine , Linköping , Sweden.; boj
University of Michigan, Ophthalmology and Visual Sciences, Kellogg Eye Center , Ann
Arbor , MI , USA.; bgj University of California San Francisco, UCSF Diabetes Center
, Department of Cell and Tissue Biology , San Francisco , CA , USA.; oh Hadassah
Hebrew University Medical Center , Department of Neurology , Jerusalem , Israel.;
auw Translational Health Science and Technology Institute, Vaccine and Infectious
Disease Research Centre , Faridabad , India.; bni University of Maryland, School of
Medicine , Department of Microbiology and Immunology , Baltimore , MD , USA.; aey
National Institute for Basic Biology, Sokendai , Okazaki , Japan.; iw Democritus
University of Thrace, Laboratory of Molecular Hematology , Alexandroupolis ,
Greece.; xk Karolinska Institute, Institute of Environmental Medicine , Stockholm ,
Sweden.; bcx University of Groningen, Department of Cell Biology, Groningen, The
Netherlands.; cbi Wayne State University, School of Medicine , Department of
Pathology , Karmanos Cancer Institute , Detroit , MI , USA.; oy Harvard Medical
School, Brigham and Women's Hospital , Department of Genetics , Division of
Genetics , Boston , MA , USA.; qg Howard Hughes Medical Institute , Boston , MA ,
USA.; bsb University of Pittsburgh , Department of Surgery , Hillman Cancer
Center , Pittsburgh , PA , USA.; oi Hallym University , Department of Anatomy and
Neurobiology , College of Medicine , Kangwon-Do , Korea.; ahs Niigata University
Graduate School of Medical and Dental Sciences, Laboratory of Biosignaling ,
Niigata , Japan.; aqb St. Jude Children's Research Hospital , Department of
Immunology , Memphis , TN , USA.; aue Tohoku University School of Medicine ,
Department of Orthopaedic Surgery , Miyagi , Japan.; uc Iowa State University ,
Department of Biomedical Science , Iowa Center for Advanced Neurotoxiclogy , Ames ,
IA , USA.; lj Florida Atlantic University, Schmidt College of Medicine , Department
of Biomedical Sciences , Boca Raton , FL , USA.; ql Hudson Institute of Medical
Research, Centre for Innate Immunity and Infectious Diseases, Clayton , Melbourne ,
Victoria , Australia.; jb Department of Medical Chemistry , Molecular Biology and
Pathobiochemistry , Budapest , Hungary.; act Merck Research Laboratories , Rahway ,
NJ , USA.; bou University of Minnesota , Department of Neuroscience , Minneapolis ,
MN , USA.; vi Jadavpur University, Life Science and Biotechnology, Kolkata , West
Bengal , India.; bgl University of Cambridge, Addenbrooke's Hospital , Department
of Medicine , Cambridge , UK.; y Albert Einstein College of Medicine , Department
of Developmental and Molecular Biology , Bronx , NY , USA.; bqb University f North
Carolina, Microbiology and Immunology , Chapel Hill , NC , USA.; bsg University of
Pittsburgh, School of Medicine , Department of Anesthesiology , Pittsburgh , PA ,
USA.; bsh University of Pittsburgh, School of Medicine , Department of Critical
Care Medicine , Pittsburgh , PA , USA.; ei Chang Gung University , Department of
Biochemistry and Molecular Biology and Graduate Institute of Biomedical Sciences ,
College of Medicine , Taoyuan County , Taiwan.; aou Shanghai University of
Traditional Chinese Medicine , Department of Biochemistry , Shanghai , China.; afn
National Institutes of Health, Laboratory of Immunoregulation, National Institute
of Allergy and Infectious Diseases , Bethesda , MD , USA.; aip Oregon Health and
Science University, Casey Eye Institute , Portland , OR , USA.; bky University of
Illinois at Urbana-Champaign , Department of Molecular and Integrative Physiology ,
Urbana , IL , USA.; bzs Vanderbilt University, School of Medicine , Department of
Molecular Physiology and Biophysics , Nashville , TN , USA.; rz INSERM U1138 ,
Paris , France.; aby Medical Center of the Johannes Gutenberg University , Mainz ,
Germany.; wb John Wayne Cancer Institute , Department of Neurosciences , Santa
Monica , CA , USA.; cbk Wayne State University, School of Medicine , Detroit , MI ,
USA.; bar University College London, MRC Laboratory for Molecular Cell Biology ,
London , UK.; bts University of São Paulo, Ribeirão Preto Medical School ,
Department of Biochemistry and Immunology , Ribeirão Preto, São Paulo , Brazil.; rn
Indiana University School of Medicine , Department of Pathology and Laboratory
Medicine , Indianapolis , IN , USA.; xf Karlsruhe Institute of Technology,
Institute of Toxicology and Genetics , Karlsruhe , Germany.; bzv Venus Medicine
Research Center (VMRC) , Baddi , Himachal Pradesh , India.; bzh US Food and Drug
Administration, National Center for Toxicology Research, Division of Microbiology ,
Jefferson , AR , USA.; avx Uniformed Services University of the Health Sciences,
Radiation Combined Injury Program, Armed Forces Radiobiology Research Institute ,
Bethesda , MD , USA.; bfu University of California San Diego, Division of
Biological Sciences , La Jolla , CA , USA.; pu Hokkaido University, Faculty of
Pharmaceutical Sciences , Sapporo , Japan.; hj Columbia University Medical Center ,
New York , NY , USA.; yd Korea Cancer Center Hospital , Department of Internal
Medicine , Seoul , Korea.; of Gyeongsang National University School of Medicine ,
Department of Biochemistry and Convergence Medical Science and Institute of Health
Sciences , JinJu , Korea.; bor University of Minnesota , Department of Biochemistry
, Molecular Biology and Biophysics , Minneapolis , MN , USA.; ccj Yonsei
University, College of Medicine, Corneal Dystrophy Research Institute ; and
Department of Ophthalmology , Seoul , Korea.; jc Dong-A University, College of
Medicine and Mitochondria Hub Regulation Center , Department of Anatomy and Cell
Biology , Busan , Korea.; cbw Wonkwang University , Department of Dental
Pharmacology , School of Dentistry , Chonbuk , Korea.; bjp University of Florida ,
Gainesville , FL , USA.; ann Seoul National University College of Medicine ,
Department of Advanced Education for Clinician-Scientists (AECS) , Seoul , Korea.;
ano Seoul National University College of Medicine , Department of Ophthalmology ,
Seoul , Korea.; bxx University of Ulsan College of Medicine, Asan Medical Center ,
Department of Surgery , Seoul , Korea.; ann Seoul National University College of
Medicine , Department of Advanced Education for Clinician-Scientists (AECS) , Seoul
, Korea.; ano Seoul National University College of Medicine , Department of
Ophthalmology , Seoul , Korea.; bzq Vanderbilt University Medical Center ,
Department of Pediatric Surgery , Nashville , TN , USA.; bnu University of Miami,
Miller School of Medicine , Department of Molecular and Cellular Pharmacology ,
Miami , FL , USA.; jd Dong-Eui University , Department of Chemistry , Busan ,
Korea.; bxd University of Toronto, Hospital for Sick Children , Toro to, Ontario ,
Canada.; bxw University of Ulsan College of Medicine, Asan Medical Center ,
Department of Biochemistry and Molecular Biology , Seoul , Korea.; aeg National
Cancer Center, Cancer Cell and Molecular Biology Branch, Division of Cancer
Biology, Research Institute , Goyang , Korea.; ok Hallym University , Department of
Microbiology , College
of Medicine , Chuncheon, Gangwon , Korea.; bce University of Alabama , Department
of Chemical and Biological Engineering , Tuscaloosa , AL , USA.; cbr Weizmann
Institute of Science , Department of Molecular Genetics , Rehovot , Israel.; oz
Harvard Medical School, Dana Farber Cancer Institute , Boston , MA , USA.; bpn
University of New Mexico, Health Sciences Center , Department of Molecular Genetics
and Microbiology , Albuquerque , NM , USA.; bub University of Sheffield ,
Department of Biomedical Sciences , Sheffield , UK.; aqj Stanford University ,
Department of Microbiology and Immunology , Stanford , CA , USA.; acs Merck KGaA,
RandD Merck Serono , Darmstadt , Germany.; bnb University of Manitoba, Institute of
Cardiovascular Sciences, College of Medicine, Faculty of Health Sciences ,
Winnipeg, Manitoba , Canada.; atc The Scripps Research Institute , Department of
Metabolism and Aging , Jupiter , FL , USA.; aua Tohoku University, Division of
Biomedical Engineering for Health and Welfare , Sendai , Japan.; bww University of
Tokyo , Department of Biotechnology , Tokyo , Japan.; aqg St. Marianna University
School of Medicine , Department of Ophthalmology , Kawasaki, Kanagawa , Japan.; ado
Nagasaki University , Department of Molecular Microbiology and Immunology ,
Graduate School of Biomedical Sciences , Nagasaki , Japan.; alv Ruhr University
Bochum, University Hospital Bergmannsheil , Department of Neurology , Heimer
Institute for Muscle Research , Bochum , Germany.; bcq University of Arizona ,
Department of Pharmacology and Toxicology , College of Pharmacy , Tucson , AZ ,
USA.; no Goethe University Medical School, Experimental Neurology , Frankfurt am
Main , Germany.; bax University Hospital Erlangen, Friedrich-Alexander-Universität
Erlangen-Nürnberg (FAU) , Erlangen , Germany.; aze Université de Montréal,
Institute for Research in Immunology and Cancer , Montréal, Québec , Canada.; ea
Centro de Investigación Príncipe Felipe , Valencia , Spain.; nt Goethe University,
Institute for Molecular Biosciences, Molecular Developmental Biology , Frankfurt ,
Hesse , Germany.; gm Chung-Shan Medical University, Institute of Medicine ,
Taichung , Taiwan.; ahh New York Institute of Technology , Department of Biomedical
Sciences , College of Osteopathic Medicine , Old Westbury , NY , USA.; bbt
University Medicine Göttingen , Department of Neurology , Göttingen , Germany.; boz
University of Montpellier, INRA, UMR 866, Dynamique Musculaire et Métabolisme ,
Montpellier , France.; cbu Westfälische Wilhelms-Universität Münster, Albert-
Schweitzer-Campus 1, Institute of Experimental Musculoskeletal Medicine , Münster ,
Germany.; ol Hallym University, Ilsong Institute of Life Science , Chuncheon ,
Korea.; ks ETH Zurich, Institute of Molecular Systems Biology , Zurich ,
Switzerland.; bke University of Graz, Institute of Molecular Biosciences,
BioTechMed Graz , Graz , Austria.; wm Juntendo University, Graduate School of
Medicine , Department of Cell Biology and Neuroscience , Tokyo , Japan.; aht
Niigata University, School of Medicine , Department of Biochemistry , Niigata ,
Japan.; ws Juntendo University , Tokyo , Japan.; atw Tianjin Medical University,
School of Pharmaceutical Sciences , Tianjin , China.; aes National Fisheries
Research and Development Institute (NFRDI) , Busan , Korea.; bcv University of
Athens , Department of Cell Biology and Biophysics , Faculty of Biology , Athens ,
Greece.; agy Nationwide Children's Hospital, Center for Microbial Pathogenesis ,
Columbus , OH , USA.; asu The Genome Analysis Centre (TGAC), Institute of Food
Research, Gut Health and Food Safety Programme , Norwich , UK.; pp Helsinki
University, Central Hospital, Medical Faculty, Division of Child Ps chiatry ,
Helsinki , Finland.; ahp Newcastle University, Campus for Ageing and Vitality,
Institute for Cell and Molecular Biosciences and Institute for Ageing , Newcastle
upon Tyne , UK.; bwf University of Texas, MD Anderson Cancer Center , Houston ,
TX , USA.; asb Temasek Life Sciences Laboratory , Singapore.; iy Democritus
University of Thrace, School of Medicine , Alexandroupolis , Greece.; brk
University of Pennsylvania Perelman School of Medicine , Department of Radiation
Oncology , Philadelphia , PA , USA.; kj Eötvös Loránd University , Department of
Anatomy , Cell and Developmental Biology , Budapest , Hungary.; kl Eötvös Loránd
University , Department of Genetics , Budapest , Hungary.; kp ETH Zurich ,
Department of Biology , Institute of Molecular Health Sciences , Zurich ,
Switzerland.; wx Kanazawa Medical University, Diabetology and Endocrinology ,
Ishikawa , Japan.; byi University of Vienna, Max F. Perutz Laboratories , Vienna ,
Austria.; aid Northwestern University, Feinberg School of Medicine , Department of
Neurology , Chicago , IL , USA.; bwq University of Texas, Southwestern Medical
Center , Department of Neuroscience , Dallas , TX.; bdk University of Belgrade,
Institute of Histology and Embryology, School of Medicine , Belgrade , Serbia.; kr
ETH Zurich, Institute of Molecular Health Sciences , Zurich , Switzerland.; he
CNRS, UMR 5534 , Villeurbanne , France.; ayu Université de Lyon, Lyon France; and
Centre de Génétique et de Physiologie Moléculaire et Cellulaire, Université Lyon 1,
Villeurbanne , France.; mr Georg-August-University Göttingen, Institute of Cellular
Biochemistry , Göttingen , Germany.; bwy University of Toledo , Department of
Biological Sciences , Toledo , OH , USA.; bar University College London, MRC
Laboratory for Molecular Cell Biology , London , UK.; od Gustave Roussy
Comprehensive Cancer Center , Villejuif , France.; qa Hôpital Européen Georges
Pompidou, AP-HP , Paris , France.; sj INSERM, Cordeliers Research Cancer , Paris ,
France.; azr Université Paris Descartes, Apoptosis, Cancer and Immunity Laboratory,
Team 11, Equipe Labellisée Ligue contre le Cancer and Cell Biology and Metabolomics
Platforms , Paris , France.; bco University of Arizona College of Medicine, Barrow
Neurological Institute, Phoenix Children's Hospital , Department of Child Health ,
Phoenix , AZ , USA.; bms University of Luxembourg, Luxembourg Center for Systems
Biomedicine , Luxembourg.; ay Babraham Institute , Cambridge , UK.; auq Tokyo
University of Science , Department of Applied Biological Science and Imaging
Frontier Center , Noda, Chiba , Japan.; blq University of Kiel , Department of
Cardiology , Kiel , Germany.; bvy University of Texas, Health Science Center at San
Antonio , Department of Urology , San Antonio , TX , USA.; boc University of
Michigan , Department of Molecular , Cellular, and Developmental Biology , Ann
Arbor , MI , USA.; bmq University of Louisville, School of Medicine , Department of
Anatomical Sciences and Neurobiology , Louisville , KY , USA.; bwt University of
the District of Columbia, Cancer Research Laboratory , Washington, DC , USA.; ub
International Center for Genetic Engineering and Biotechnology, Immunology Group ,
New Delhi , India.; lo George Washington University, Department of Biochemistry and
Molecular Medicine, Washington, DC , USA.; bug University of South Australia and SA
Pathology, Centre for Cancer Biology, Adelaide , SA , Australia.; aqc St. Jude
Children's Research Hospital , Department of Pathology , Memphis , TN , USA.; aet
National Health Research Institutes , Institute of Molecular and Genomic Medicine,
Miaoli , Taiwan.; bey University of California Davis, Cancer Center , Davis , CA ,
USA.; and Sapporo Medical University School of Medicine , Department of
Pharmacology , Sapporo , Japan.; hh Columbia University Medical Center , Department
of Neurology , New York , NY , USA.; aii Ohio State University , Department of
Molecular and Cellular Biochemistry , Columbus , OH , USA.; zp Linköping University
, Department of Medical and Health Sciences , Lin öping , Sweden.; acy Monash
University , Department of Biochemistry and Molecular Biology , Victoria ,
Australia.; acz Monash University , Department of Microbiology , Victoria ,
Australia.; xn Keimyung University , Daegu , Korea.; anw Seoul National University,
Protein Metabolism Medical Research Center and Department of Biomedical Sciences ,
College of Medicine , Seoul , Korea.; bio University of Crete, School of Medicine ,
Department of Infectious Diseases , Heraklion, Crete , Greece.; amr Sanford
Consortium for Regenerative Medicine , La Jolla , CA , USA.; bft University of
California San Diego, Departments of Cellular and Molecular Medicine,
Neurosciences, and Pediatrics, Division of Biological Sciences Institute for
Genomic Medicine , La Jolla , CA , USA.; su Center for Infection and Immunity of
Lille, Institut Pasteur de Lille, CNRS, INSERM, Lille Regional University Hospital
Centre, Lille University , Lille , France.; ro Indiana University School of
Medicine, Richard L. Roudebush VA Medical Center, Division of Pulmonary, Critical
Care, Sleep and Occupational Medicine , Indianapolis , IN , USA.; bys University of
Wisconsin , Department of Ophthalmology and Visual Sciences , McPherson Eye
Research Institute , Madison , WI , USA.; bwl University of Texas, Medical School
at Houston, Division of Cardiovascular Medicine , Department of Medicine ,
Houston , TX , USA.; bxl University of Tromsø - The Arctic University of Norway ,
Department of Medical Biology , Tromsø , Norway.; bbk University Lille, INSERM, CHU
Lille, Institut Pasteur de Lille, U1011, EGID , Lille , France.; bcn University of
Arizona Cancer Center , Department of Medicine , Tucson , AZ , USA.; bvi University
of Sydney , Department of Pathology and Bosch Institute , Sydney, New South Wales ,
Australia.; bea University of Bristol, School of Biochemistry , Bristol , UK.; ll
Fondazione IRCCS Istituto Nazionale dei Tumori , Department of Experimental
Oncology and Molecular Medicine , Milan , Italy.; ayh Université Bourgogne Franche-
Comté, Agrosup Dijon, UMR PAM, Équipe Vin, Aliment, Microbiologie,
Stress , Dijon , France.; cm Brown University , Department of Molecular Biology ,
Cell Biology and Biochemistry , Providence , RI , USA.; sg INSERM U964, CNRS
UMR7104, Université de Strasbourg , Department of Translational Medecine , Institut
de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC) , Illkirch , France.;
arn Tampere University Hospital , Department of Gastroenterology and Alimentary
Tract Surgery , Tampere , Finland.; byk University of Virginia , Department of Cell
Biology , Charlottesville , VA , USA.; awk Universidad de Chile, Advanced Center
for Chronic Diseases (ACCDiS) , Santiago , Chile.; bwp University of Texas,
Southwestern Medical Center , Department of Internal Medicine , Dallas , TX.; arq
Technion-Israel Institute of Technology, Unit of Anatomy and Cell Biology, The Ruth
and Bruce Rappaport Faculty of Medicine , Haifa , Israel.; cf Brigham and Women's
Hospital, Ann Romney Center for Neurologic Diseases , Department of Neurology ,
Harvard Medical School , Boston , MA , USA.; aai Macau University of Science and
Technology, State Key Laboratory of Quality Research in Chinese Medicine , Macau ,
China.; px Hong Kong Polytechnic University , Department of Health Technology and
Informatics , Faculty of Health and Social Sciences , Kowloon, Hong Kong.; bxd
University of Toronto, Hospital for Sick Children , Toronto, Ontario , Canada.; bqe
University of Nottingham, School of Life Sciences , Nottingham , UK.; hz Consejo
Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca,
Experimental Therapeutics and Translational Oncology Program, Instituto de Biología
Molecular y Celular del Cáncer , Salamanca , Spain.; to Instituto de Investigación
Biomédica de Salamanca (IBSAL) , Hospital Universitario de Salamanca, Salamanca ,
Spain.; qu ICM, Institut de Recherche en Cancérologie de Montpellier ,
Montpellier , France.; sd INSERM U896 , Montpellier , France.; sr Institut du
Cancer de Montpellier , Montpellier , France.; bfk University of California
Riverside , Department of Cell Biology and Neuroscience , Riverside , CA , USA.;
azy Université Paris Diderot, Unité Biologie Fonctionnelle et Adaptative - CNRS UMR
8251 , Paris , France.; bab Université Paris-Sud, CEA, CNRS, Institute for
Integrative Biology of the Cell , Gif-sur-Yvette Cedex , France.; aam Mahidol
University , Department of Anatomy , Faculty of Science , Bangkok , Thailand.; sx
Institut Pasteur, INSERM, Biology of Infection Unit , Paris , France.; ans Seoul
National University, College of Pharmacy and Research Institute of Pharmaceutical
Science , Seoul , Korea.; fb China Medical University , Department of
Microbiology , Taichung , Taiwan.; zc Olivia Newton-John Cancer Research
Institute , Melbourne , Victoria , Australia.; ze La Trobe University , Department
of Chemistry and Physics , Melbourne , Victoria , Australia.; zf La Trobe
University, School of Cancer Medicine , Melbourne , Victoria , Australia.; wv KAIST
, Department of Biological Sciences , Daejon , Korea.; yc Konkuk University, School
of Medicine , Department of Anatomy , Seoul , Korea.; age National Taiwan
University , Department of Life Science and Center for Biotechnology , Taipei ,
Taiwan.; yf Korea University , Department of Life Science and Biotechnology , Seoul
, Korea.; bfm University of California San Diego , Department of Medicine , La
Jolla , CA , USA.; ahm New York University , Department of Psychiatry , New York NY
; and Center for Dementia Research, Nathan S. Kline Institute , Orangeburg , NY ,
USA.; bob University of Michigan , Department of Molecular and Integrative
Physiology , Ann Arbor , MI , USA.; rd Incheon National University, Division of
Life Siences , Incheon , Korea.; arf Yonsei University College of Medicine,
Severans Biomedical Science Institute and Department of Internal Medicine , Seoul ,
Korea.; cce Yale University School of Medicine, Section of Pulmonary, Critical Care
and Sleep Medicine , New Haven , CT , USA.; aat Massachusetts General Hospital and
Harvard Medical School, Cutaneous Biology Research Center , Charlestown , MA.; anq
Seoul National University College of Medicine, Neuroscience Research Institute ,
Department of Medicine , Seoul , Korea.; aev National Health Research Institutes,
Institute of Biotechnology and Pharmaceutical Research , Miaoli County , Taiwan.;
xa Kansas State University, Division of Biology , Manhattan , KS , USA.; db
Catholic University of Korea , Seoul , Korea.; kq ETH Zurich, Institute of
Biochemistry , Zurich , Switzerland.; ku ETH Zurich, ScopeM (Scientific Center for
Optical and Electron Microscopy) , Zurich , Switzerland.; ye Korea University ,
Department of Biotechnology , BK21-PLUS Graduate School of Life Sciences and
Biotechnology , Seoul , Korea.; jf Duke University , Department of Medicine , Human
Vaccine Institute , Durham , NC , USA.; es Ditmanson Medical Foundation Chia-Yi
Christian Hospital, Center for Translational Medicine , Chiayi City , Taiwan.; bsc
University of Pittsburgh , Department of Surgery , Pittsburgh , PA , USA.; afo
National Institutes of Health, National Cancer Institute, Urologic Oncology
Branch , Bethesda , MD , USA.; bjq University of Florida, Institute on Aging ,
Gainesville , FL , USA.; cj British Columbia Cancer Agency, Terry Fox Laboratory ,
Vancouver, BC , Canada.; bab Université Paris-Sud, CEA, CNRS, Institute for
Integrative Biology of the Cell , Gif-sur-Yvette Cedex , France.; avd Tsinghua
University, Zhou Pei-Yuan Center for Applied Mathematics , Beijing , China.; ly
Fudan University Shanghai Medical College , Department of Biochemistry and
Molecular Biology , School of Basic Medical Sciences, Institute of Biomedical
Sciences , Shanghai , China.; mh Geisel School of Medicine at Dartmouth ,
Department of Microbiology and Immunology , Lebanon , NH , USA.; og Hadassah Hebrew
University Medical Center, Endocrinology and Metabolism Service , Department of
Medicine , Jerusalem , Israel.; bir University of Debrecen, Faculty of Pharmacy ,
Department of Pharmacology , Debrecen , Hungary.; dv Centre National de la R
cherche Scientifique, Sorbonne Universités UPMC Univ Paris 06, UMR 8226,
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de
Biologie Physico-Chimique , Paris , France.; acl Medical University of South
Carolina, Departments of Drug Discovery and Biomedical Sciences , and Biochemistry
and Molecular Biology , Charleston , SC , USA.; bkg University of Heidelberg,
Center for Molecular Biology , Heidelberg , Germany.; qc Hôpital Paul Brousse -
Hôpitaux Universitaires Paris-Sud, Biochimie et Oncogénétique , Villejuif ,
France.; ny Guangzhou Medical University , Department of Human Anatomy , School of
Basic Science , Guangzhou, Guangdong , China.; axc Universidad Federal do Rio
Grande do Sul (UFRGS) , Department of Biophysics and Center of Biotechnology ,
Porto Alegre , Brazil.; brt University of Pisa , Department of Translational
Research and New Technologies in Medicine and Surgery , Pisa , Italy.; abf Mayo
Clinic, Division of Nephrology and Hypertension , Rochester , MN , USA.; btd
University of Rome "Tor Vergata" , Department of Biology , Rome , Italy.; akc
Perelman School of Medicine at the University of Pennsylvania , Department of
Genetics , Philadelphia , PA , USA.; bjy University of Glasgow, Cancer Research UK
Beatson Institute , Glasgow , UK.; bjz University of Glasgow, Institute of Cancer
Sciences , Glasgow , UK.; qh Howard Hughes Medical Institute , Dallas , TX.; bvr
University of Texas , Southwestern Medical Center, Department of Internal
Medicine , Center for Autophagy Research, Dallas , TX , USA.; avm UCL Institute of
Neurology , Department of Molecular Neuroscience , London , UK.; bsy University of
Reading, School of Pharmacy, Whiteknights , Reading , UK.; bzd University Paul
Sabatier, INSERM U1048 , Toulouse , France.; bmp University of Louisville, James
Graham Brown Cancer Center , Department of Medicine , Department of Pharmacology
and Toxicology , Louisville , KY , USA.; byq Washington University in St Louis,
Department of Biology, St. Louis, MO , USA.; ags National University of Singapore ,
Department of Biological Sciences , Singapore.; bzr Vanderbilt University ,
Department of Neurology , Nashville , TN , USA.; fe Chinese Academy of Medical
Sciences and Peking Union Medical College, Institute of Medicinal Biotechnology ,
Beijing , China.; kd Emory University, School of Medicine , Department of
Pharmacology , Atlanta , GA , USA.; pw Hong Kong Baptist University, School of
Chinese Medicine , Kowloon Tong, Hong Kong.; aqz Sun Yat-Sen University ,
Department of Pharmacology and Toxicology , School of Pharmaceutical Sciences ,
Guangzhou , China.; fj Chinese Academy of Sciences, Division of Medical Physics,
Institute of Modern Physics , Lanzhou, Gansu Province , China.; abl McGill
University , Department of Neuroscience , Montreal Neurological Institute ,
Montreal, QC , Canada.; fq Chinese Academy of Sciences, Key Laboratory of
Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology,
Shanghai Institutes for Biological Sciences , Shanghai , China.; cq Capital Medical
University, Center for Medical Genetics, Beijing Children's Hospital , Beijing ,
China.; fv Chinese Academy of Sciences, State Key Laboratory of Stem Cell and
Reproductive Biology, Institute of Zoology , Beijing , China.; jr East China Normal
University , Shanghai , China.; ang Second Hospital of Lanzhou University, Key
Laboratory of Digestive System Tumors , Gansu , China.; atk Third Military Medical
University , Department of Biochemistry and Molecular Biology , Chongqing , China.;
bux University of Southern California, Keck School of Medicine , Department of
Molecular Microbiology and Immunology , Los Angeles , CA , USA.; ahh New York
Institute of Technology , Department of Biomedical Sciences , College of
Osteopathic Medicine
, Old Westbury , NY , USA.; apv Southern Medical University , Department of
Cardiology , Nanfang Hospital , Guangzhou , China.; bhp University of Coimbra,
Center for Neuroscience and Cell Biology and Faculty of Pharmacy , Coimbra ,
Portugal.; acf Medical University o Lodz , Department of Molecular Pathology and
Neuropathology , Lodz , Poland.; dg Center for Dementia Research, Nathan S. Kline
Institute , Orangeburg , NY , USA.; bnx University of Michigan Medical School ,
Department of Pathology , Ann Arbor , MI , USA.; ya Konkuk University , Department
of Veterinary Medicine , Seoul , Korea.; aga National Neuroscience Institute ,
Singapore.; agv National University of Singapore , Department of Physiology , Yong
Loo Lin School of Medicine , Singapore.; gj Chungnam National University, School of
Medicine , Department of Biochemistry , Infection Signaling Network Research
Center, Cancer Research Institute , Daejeon , Korea.; bsp University of Porto,
Cancer Drug Resistance Group, IPATIMUP - Institute of Molecular Pathology and
Immunology , Porto , Portugal.; bsr University of Porto , Department of Pathology
and Oncology , Faculty of Medicine , Porto , Portugal.; bss University of Porto,
i3S-Instituto de Investigação e Inovação em Saúde , Porto , Portugal.; xc Kaohsiung
Medical University , Graduate Institute of Medicine , Kaohsiung , Taiwan.; agd
National Sun Yat-Sen University, Department of Biological Sciences , Kaohsiung ,
Taiwan.; ark Taipei Medical University , Department of Microbiology and
Immunology , Institute of Medical Sciences , Taipei , Taiwan.; apn Soochow
University, School of Pharmaceutical Science , Department of Pharmacology ,
Laboratory of Aging and Nervous Diseases , Su Zhou, Jiangsu Province , China.; hk
Columbia University, College of Physicians and Surgeons , Department of
Pediatrics , New York , NY , USA.; ccu Zhejiang University, Institute of
Agriculture and Biotechnology , Hangzhou , China.; mm Genentech Inc. , Department
of Translational Oncology , South San Francisco , CA , USA.; ej Chang Gung
University , Department of Biochemistry , College of Medicine , Taoyuan , Taiwan.;
aik Ohio State University , Department of Surgery , Davis Heart and Lung Research
Institute , Columbus , OH , USA.; cby Xiamen University, School of Life Sciences ,
Fujian , China.; agh National Taiwan University , Department of Pharmacology ,
College of Medicine , Taipei , Taiwan.; ael National Cheng Kung University ,
Department of Microbiology and Immunology , College of Medicine , Tainan , Taiwan.;
aaa Lovelace Respiratory Research Institute, Molecular Biology and Lung Cancer
Program , Albuquerque , NM , USA.; avq UFRJ, Instituto de Biofisica Carlos Chagas
Filho , Rio de Janeiro , Brazil.; bki University of Helsinki, Biomedicum , Helsinki
, Finland.; bnq Cell Signalling and Cell Death Division, and University of
Melbourne, Walter and Eliza Hall Institute of Medical Research , Department of
Medical Biology , Parkville , Victoria , Australia.; bkd University of Göttingen ,
Department of Neurology , Göttingen , Germany.; gg Christian-Albrechts-University
of Kiel , Department of Nephrology and Hypertension , Kiel , Germany.; ms George
Mason University , Manassas , VA , USA.; bng University of Maryland, School of
Medicine , Department of Anesthesiology , Baltimore , MD , USA.; bld University of
Iowa , Department of Health and Human Physiology , Iowa City , IA , USA.; bmv
University of Manchester, Breakthrough Breast Cancer Research Unit, Manchester
Centre for Cellular Metabolism , UK.; jh Duke University , Department of
Ophthalmology , Durham , NC , USA.; apb Sichuan University, State Key Laboratory of
Biotherapy/Collaborative Innovation Center of Biotherapy, West China Hospital ,
Chengdu , China.; ani Second Military Medical University , Department of
Pharmacology , Shanghai , China.; apk Soochow University , Department of
Neurology , Second Affiliated Hospital of Soochow University and Institute of
Neuroscience , Suzhou , China.; bok University of Michigan, School of Dentistry ,
Department of Biologic and Materials Sciences , Ann Arbor , MI , USA.; aeq National
Chung Hsing University, Institute of Molecular Biology , Taichung , Taiwan.; ex
China Academy of Chinese Medical Sciences, Institute of Basic Medical Sciences of
Xiyuan Hospital , Beijing , China.; bfw University of Californi San Diego, Division
of Biological Sciences, Section of Molecular Biology , La Jolla , CA , USA.; ef
Chang Gung Memorial Hospital , Department of Pathology , Chiayi , Taiwan.; apa
Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of Ministry
of Education, College of Life Science , Chengdu, Sichuan , China.; asf Texas A&M
Health Science Center, Institute of Biosciences and Technology , Houston , TX ,
USA.; aai Macau University of Science and Technology, State Key Laboratory of
Quality Research in Chinese Medicine , Macau , China.; ip Dalian Medical
University, Institute of Cancer Stem Cell , Dalian , China.; aso The First
Affiliated Hospital of Anhui Medical University , Department of Pulmonary , Anhui
Geriatric Institute , Anhui , China.; ate The Second Hospital Affiliated to
Guangzhou Medical University, Guangzhou Institute of Cardiovascular Disease ,
Guangzhou, Guangdong Province , China.; apw Southern Medical University, School of
Pharmaceutical Sciences, Guangzhou , Guangdong , China.; ccz Zhejiang University,
School of Medicine , Department of Biochemistry , Hangzhou, Zhejiang , China.; bwb
University of Texas, MD Anderson Cancer Center , Department of Genitourinary
Medical Oncology , Houston , TX , USA.; aob Shandong University, School of Life
Sciences , Jinan , China.; ccu Zhejiang University, Institute of Agriculture and
Biotechnology , Hangzhou , China.; adv Nanjing University School of Medicine,
Jinling Hospital , Department of Neurology , Nanjing , China.; boc University of
Arbor , MI , USA.; bog University of Michigan, Life Sciences Institute , Ann
Arbor , MI , USA.; lw Freshwater Aquaculture Collaborative Innovation Center of
Hubei Province , Wuhan , China.; qj Huazhong Agricultural University , Department
of Aquatic Animal Medicine , College of Fisheries , Wuhan , China.; bvr University
of Texas , Southwestern Medical Center, Department of Internal Medicine , Center
for Autophagy Research, Dallas , TX , USA.; avb Tsinghua University, School of Life
Sciences , Beijing , China.; qk Huazhong University of Science and Technology ,
Department of Biomedical Engineering , College of Life Science and Technology ,
Wuhan, Hubei , China.; atv Tianjin Medical University , Department of Immunology ,
Tianjin Key Laboratory of Medical Epigenetics , Tianjin , China.; lk Florida
International University , Department of Dietetics and Nutrition , Miami , FL ,
USA.; bak University Bourgogne Franche Comté, EA 7270/INSERM , Dijon , France.; agp
National University of Ireland, Apoptosis Research Centre , Galway , Ireland.; cbf
Washington University, School of Medicine, Division of Endocrinology, Metabolism
and Lipid Research , Department of Medicine , St. Louis , MO , USA.; agp National
University of Ireland, Apoptosis Research Centre , Galway , Ireland.; na Georgia
Regents University, Cancer Center , Department of Medicine , Augusta , GA , USA.;
agw National University of Singapore, Yong Loo Lin School of Medicine , Department
of Biochemistry , Singapore.; ki Emory University, Winship Cancer Institute ,
Department of Hematology and Medical Oncology , Atlanta , GA , USA.; aqr
Stellenbosch University , Department of Physiological Sciences , Stellenbosch ,
South Africa.; awu Universidad de Oviedo, Instituto Universitario de Oncología ,
Departamento de Bioquímica y Biología Molecular , Oviedo , Spain.; bda University
of Barcelona , Department of Biochemistry and Molecular Genetics , Hospital Clínic,
IDIBAPS-CIBERehd , Barcelona , Spain.; hs Complutense University, Instituto de
Investigaciones Sanitarias San Carlos (IdISSC) , Department of Biochemistry and
Molecular Biology I , School of Biology , Madrid , Spain.; bwa University of Texas,
MD Anderson Cancer Center , Department of Bioinformatics and Computational
Biology , Houston , TX , USA.; bwg University of Texas, MD Anderson Cancer Center,
The Proteomics and Metabolomics Core Facility , Houston , TX , USA.; kj Eötvös
Loránd University , Department of Anatomy , Cell and Dev lopmental Biology ,
Budapest , Hungary.; zn Medical University of Silesia, ENT Department, School of
Medicine, Katowice, Poland.; bsd University of Pittsburgh , Department of Surgery ,
University of Pittsburgh Cancer Institute , Pittsburgh , PA , USA.; ahq Newcastle
University, The Medical School, Institute of Cellular Medicine , Newcastle upon
Tyne , UK.; bsi University of Pittsburgh, School of Medicine , Department of
Immunology , Pittsburgh , PA , USA.; ato Thomas Jefferson University Hospitals ,
Department of Radiation Oncology , Philadelphia , PA , USA.; bmt University of
Macau, State Key Lab of Quality Research in Chinese Medicine, Institute of Chinese
Medical Sciences , Macao , China.; am Alpert Medical School of Brown University,
Vascular Research Laboratory, Providence Veterans Affairs Medical Center ,
Department of Medicine , Providence , RI , USA.; cca Xi'an Jiaotong University
Health Science Center , Department of Biochemistry and Molecular Biology , School
of Basic Medical Sciences , Shaanxi , China.; ake Pfizer Inc., Drug Safety Research
and Development , San Diego , CA , USA.; gu City University of Hong Kong ,
Department of Biomedical Sciences , Kowloon Tong, Hong Kong , China.; ru INSERM
U1065, C3M, Team 2 , Nice , France.; bez University of California Davis ,
Department of Medical Microbiology and Immunology , School of Medicine , Davis , CA
, USA.; bvd University of St Andrews, School of Medicine , St Andrews, Fife
, UK.; boo Life and Health Sciences Research Institute (ICVS), School of Health
Sciences, University of Minho, Braga, Portugal.; boq ICVS/3B's - PT Government
Associate Laboratory, Braga/Guimarães, Portugal.; df Cedars-Sinai Medical Center,
VAGLAHS-UCLA, Pancreatic Research Group , Los Angeles , CA , USA.; bnx University
of Michigan Medical School , Department of Pathology , Ann Arbor , MI , USA.; aux
Trev and Joyce Deeley Research Centre; and University of Victoria, BC Cancer
Agency; and Department of Biochemistry and Microbiology , Victoria, BC , Canada.;
bij University of Copenhagen, Biotech Research and Innovative Center (BRIC) ,
Copenhagen , Denmark.; beg University of British Columbia , Department of Pathology
and Laboratory Medicine , James Hogg Research Centre , Vancouver, BC , Canada.; bll
University of Kentucky, College of Medicine , Department of Pharmacology and
Nutritional Sciences , Lexington , KY , USA.; akf Plymouth University, Peninsula
School of Medicine and Dentistry , Plymouth , UK.; brf University of Palermo ,
Dipartimento di Scienze e Tecnologie Biologiche , Chimiche e Farmaceutiche
(STEBICEF) , Palermo , Italy.; ala Queen's University of Belfast, Centre for
Experimental Medicine , Belfast , UK.; aik Ohio State University , Department of
Surgery , Davis Heart and Lung Research Institute , Columbus , OH , USA.; aoi
Shanghai Jiao Tong University, School of Medicine, Center for Reproductive
Medicine, Renji Hospital , Shanghai , China.; asp The First Affiliated Hospital of
Harbin Medical University , Department of General Surgery , Harbin, Heilongjiang
Province , China.; atu Tianjin Medical University , Department of Biochemistry and
Molecular Biology , School of Basic Medical Sciences, Tianjin Key Laboratory of
Medical Epigenetics , Tianjin , China.; bts University of São Paulo, Ribeirão Preto
Medical School , Department of Biochemistry and Immunology , Ribeirão Preto, São
Paulo , Brazil.; btr University of São Paulo, Institute of Biomedical Science ,
Department of Cell and Developmental Biology , São Paulo, SP , Brazil.; ac Albert
Einstein College of Medicine , Department of Pathology , Bronx , NY , USA.; ue Iowa
State University, Roy J. Carver Department of Biochemistry, Biophysics, and
Molecular Biology , Ames , IA , USA.; bzo Van Andel Research Institute, Laboratory
of Systems Biology , Grand Rapids , MI , USA.; bhe University of Chicago , The Ben
May Department for Cancer Research , Chicago , IL , USA.; ccd Yale University
School of Medicine , Department of Microbial Pathogenesis and Howard Hughes Medical
Institute , New Haven , CT , USA.; bct University of Arka sas for Medical
Sciences , Department of Pharmacology/Toxicology , Little Rock , AR , USA.; bke
University of Graz, Institute of Molecular Biosciences, BioTechMed Graz , Graz ,
Austria.; asd Temple University, School of Medicine , Department of Biochemistry ;
and Center for Translational Medicine , Philadelphia , PA , USA.; y Albert Einstein
College of Medicine , Department of Developmental and Molecular Biology , Bronx ,
NY , USA.; cv Case Western Reserve University , Department of Ophthalmology and
Visual Sciences , Cleveland , OH , USA.; bwx University of Tokyo, Institute of
Molecular and Cellular Biosciences , Tokyo , Japan.; bjn University of Florida ,
Department of Pediatrics/Genetics and Metabolism , Gainesville , FL , USA.; bud
University of Siena , Department of Molecular and Developmental Medicine , Siena ,
Italy.; yn KU Leuven, Laboratory for Cell Death Research and Therapy , Department
of Cellular and Molecular Medicine , Campus Gasthuisberg , Leuven , Belgium.; awd
Universidad Autónoma de Madrid , Departamento de Biología , Madrid , Spain.; bnp
University of Medicine and Dentistry of New Jersey, Cellular and Molecular
Signaling , Newark , NJ , USA.; rh Indian Institute of Technology Kharagpur ,
Department of Biotechnology , Kharagpur , India.; cah San Raffaele Scientific
Institute, European Institute for Research in Cystic Fibrosis , Milan , Italy.; avv
UMRS 1138, Centre de Recherche des Cordeliers , Paris , France.; alx Rush
University Medical Center , Department of Anatomy and Cell Biology , Chicago , IL ,
USA.; ace Medical University of Graz, Institute of Molecular Biology and
Biochemistry, Centre of Molecular Medicine , Graz , Austria.; vd Istituto Superiore
di Sanità , Rome , Italy.; amp San Raffaele Institute, Dept. of Therapeutic
Research and Medicine Evaluation , Sulmona, L'Aquila , Italy.; aiq Oregon Health
and Science University, Knight Cardiovascular Institute , Portland , OR , USA.; oe
Gustave Roussy Institute , Villejuif , France.; bnv University of Miami, Miller
School of Medicine, Sylvester Comprehensive Cancer Center , Miami , FL , USA.; btv
University of Science and Technology of China , Anhui , China.; pa Harvard Medical
School, Dana Farber Cancer Institute and Beth Israel Deaconess Medical Center ,
Department of Radiation Oncology , Boston , MA , USA.; jp DZNE, German Center for
Neurodegenerative Diseases, and CAESAR Research Center , Bonn , Germany.; bpn
University of New Mexico, Health Sciences Center , Department of Molecular Genetics
and Microbiology , Albuquerque , NM , USA.; caj San Raffaele Scientific Institute ,
Milan , Italy.; avu UMR CNRS 5286, INSERM 1052, Cancer Research Center of Lyon ,
Lyon , France.; avn UCL Institute of Neurology , London , UK.; bsx University of
Reading, School of Pharmacy , Reading , UK.; aau Massachusetts General Hospital and
Harvard Medical School , Department of Molecular Biology ; Department of Genetics ,
Boston , MA , USA.; kc Emory University, School of Medicine , Department of
Pharmacology and Neurology , Atlanta , GA , USA.; arb Sun Yat-Sen University,
School of Chemistry and Chemical Engineering , Guangzhou , China.; asn The
Feinstein Institute for Medical Research, North Shore LIJ Health System, Litwin-
Zucker Research Center for the Study of Alzheimer's Disease , New York , NY , USA.;
bom University of Milan , Department of Health Sciences , Milan , Italy.; ajm Paris
Descartes University-Sorbonne Paris Cité, Imagine Institute , Paris , France.; anj
Second University of Naples , Department of Biochemistry and Biophysics , Naples ,
Italy.; bgi University of California San Francisco, School of Medicine , Department
of Pathology , San Francisco , CA , USA.; anm Semmelweis University, Institute of
Human Physiology and Clinical Experimental Research , Budapest , Hungary.; bbp
University Medical Center Utrecht , Department of Cell Biology , Groningen , The
Netherlands.; buy University of Southern California, Keck School of Medicine, Eli
and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research ,
Department of Cell and Neurobiolo y , Los Angeles , CA , USA.; uq IRCE, Institut
d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) , Barcelona , Spain.; ha
CNR, Institute of Cell Biology and Neurobiology and IRCCS Santa Lucia Foundation ,
Rome , Italy.; bqx University of Oviedo , Department of Animal Physiology , Faculty
of Medicine, Campus del Cristo , Oviedo , Spain.; bdl University of Belgrade,
Institute of Medical and Clinical Biochemistry, Faculty of Medicine , Belgrade ,
Serbia.; bli University of Kansas and University of Kansas Cancer Center ,
Departments of Molecular Biosciences and Radiation Oncology , Lawrence , KS , USA.;
bdu University of Bologna , Department of Biomedical and Neuromotor Sciences ,
Bologna , Italy.; byi University of Vienna, Max F. Perutz Laboratories , Vienna ,
Austria.; bzo Van Andel Research Institute, Laboratory of Systems Biology , Grand
Rapids , MI , USA.; auy Trinity College Dublin , Department of Genetics , The
Smurfit Institute , Dublin , Ireland.; yl KU Leuven , Department of Cellular and
Molecular Medicine , Leuven , Belgium.; dz Centro de Biologia Molecular "Severo
Ochoa" (UAM/CSIC) , Department of Virology and Microbiology , Madrid , Spain.; tp
Instituto de Investigaciones Biomédicas Alberto Sols, CSIC/UAM, Centro de
Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd) ,
Madrid , Spain.; bpb University of Montpellier, UMR 5554 , Montpellier , France.;
bcm University of Antwerp, Laboratory of Physiopharmacology, Wilrijk , Antwerp ,
Belgium.; bbj National Institute of Environmental Health Sciences, Immunity,
Inflammation, and Disease Laboratory, Research Triangle Park, NC, USA.; af Albert
Einstein College of Medicine, Departments of Medicine and Molecular Pharmacology ,
Bronx , NY , USA.; ats Thomas Jefferson University , Philadelphia , PA , USA.; eb
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco,
AC, Unidad de Biotecnología Médica y Farmacéutica , Guadalajara , Jalisco ,
México.; bzm Vall d'Hebron Research Institute-CIBERNED, Neurodegenerative Diseases
Research Group , Barcelona , Spain.; amw São Paulo University , Biochemistry
Department ; and Santo Amaro University, Life Sciences , São Paulo , Brazil.; t
Akita University, Graduate School of Medicine , Akita , Japan.; bhd University of
Chicago, Pritzker School of Medicine , Department of Neurology , Chicago , IL ,
USA.; up IRCCS-MultiMedica , Milan , Italy.; axu Università di Salerno ,
Dipartimento di Medicina e Chirurgia , Baronissi, Salerno , Italy.; vc Istituto
Superiore di Sanità , Department of Therapeutic Research and Medicine , Evaluation
Section of Cell Aging, Degeneration and Gender Medicine , Rome , Italy.; aqj
Stanford University , Department of Microbiology and Immunology , Stanford , CA ,
USA.; ys Kyoto Prefectural University of Medicine , Department of Cardiovascular
Medicine , Graduate School of Medical Science , Kyoto , Japan.; cch Yokohama City
University Graduate School of Medicine , Department of Human Genetics , Yokohama ,
Japan.; xx Kobe University, Graduate School of Medicine , Department of Orthopaedic
Surgery , Hyogo , Japan.; et Chiba University , Department of Nanobiology
, Chiba , Japan.; ais Osaka Prefecture University, Graduate School of Life and
Environmental Science , Osaka , Japan.; afg National Institute on Aging, Intramural
Research Program, Laboratory of Neurosciences , Baltimore , MD , USA.; ahf
Neurounion Biomedical Foundation , Santiago , Chile.; bge FONDAP Center for
Geroscience, Brain Health and Metabolism, Santiago, Chile.; bhh University of
Chile, Biomedical Neuroscience Institute , Santiago , Chile.; cag Vita-Salute San
Raffaele University, San Raffaele Scientific Institute, Autoimmunity and Vascular
Inflammation Unit , Milan , Italy.; bos University of Minnesota , Department of
Genetics , Cell Biology and Development , Minneapolis , MN , USA.; blv University
of Lausanne , Department of Biochemistry , Epalinges , Switzerland.; abm McGill
University , Department of Pharmacology and Therapeutics , Montreal, Quebec ,
Canada.; aw Austral U iversity-CONICET, Gene and Cell Therapy Laboratory , Pilar ,
Buenos Aires , Argentina.; dg Center for Dementia Research, Nathan S. Kline
Institute , Orangeburg , NY , USA.; cb Boston University , Department of Biology ,
Boston , MA , USA.; ij Dalhousie University , Department of Microbiology and
Immunology , Halifax, Nova Scotia , Canada.; xi Karolinska Institute , Department
of Microbiology , Tumor and Cell Biology , Stockholm , Sweden.; byt University of
Wisconsin, School of Medicine and Public Health , Department of Cell and
Regenerative Biology , Carbone Cancer Center , Madison , WI , USA.; ban University
College Cork, Cork Cancer Research Centre, BioSciences Institute, Co. Cork ,
Ireland.; jg Duke University , Department of Molecular Genetics and Microbiology ,
Durham , NC , USA.; bjz University of Glasgow, Institute of Cancer Sciences ,
Glasgow , UK.; sb INSERM U830, Stress and Cancer Laboratory, Institut Curie , Paris
, France.; bch University of Amsterdam, Academic Medical Center, Laboratory of
Experimental Oncology and Radiobiology , Amsterdam, North Holland , The
Netherlands.; asa Telethon Institute of Genetics and Medicine (TIGEM) , Pozzuoli,
Naples , Italy.; bvj University of Szeged , Department of Medical Microbiology and
Immunobiology , Szeged, Csongrád , Hungary.; azw Université Paris Descartes-
Sorbonne Paris Cité, Institut Necker Enfants-Malades (INEM), INSERM U1151-CNRS UMR
8253 , Paris , France.; bcr University of Arkansas for Medical Sciences ,
Department of Cardiology , Little Rock , AR , USA.; btw University of Science and
Technology of China, CAS Key Laboratory of Innate Immunity and Chronic Disease,
School of Lifesciences , Hefei, Anhui , China.; akw Queen Mary University of
London, Blizard Institute , Department of Neuroscience and Trauma , London , UK.;
bcj University of Amsterdam , Department of Medical Biochemistry , Academic Medical
Center , Amsterdam , The Netherlands.; gv City University of New York , Department
of Biology , Queens College and The Graduate Center , Flushing , NY , USA.; adm MRC
Toxicology Unit , Leicester , UK.; bti University of Rome "Tor Vergata" ,
Department of Surgery and Experimental Medicine , Rome , Italy.; btf University of
Rome "Tor Vergata" , Department of Chemistry , Rome , Italy.; axl Universidade
Estadual do Norte Fluminense Darcy Ribeiro, Centro de Biociencias e Biotecnologia,
Lab Biologia Celular e Tecidual, Setor de Toxicologia Celular, Campos dos
Goytacazes , Rio de Janeiro , Brazil.; qe Hospital Universitario Ramón y Cajal,
CIBERNED , Neurobiology Department , Madrid , Spain.; bxb University of Toronto ,
Department of Molecular Genetics , Toronto, Ontario , Canada.; nn Girona Biomedical
Research Institute (IDIBGI), Catalan Institute of Oncology (ICO) , Catalonia ,
Spain.; qo iBET, Instituto de Biologia Experimental e Tecnológica , Oeiras ,
Portugal.; tt Instituto de Tecnologia Química e Biológica António Xavier,
Universidade Nova de Lisboa , Oeiras , Portugal.; cca Xi'an Jiaotong University
Health Science Center , Department of Biochemistry and Molecular Biology , School
of Basic Medical Sciences , Shaanxi , China.; aod Shanghai Institute of Materia
Medica, Division of Antitumor Pharmacology , Shanghai , China.; im Dalian Medical
University, Cancer Center, Institute of Cancer Stem Cell , Dalian, Liaoning
Province , China.; btk University of Rome "Tor Vergata" , Department of Systems
Medicine , Rome , Italy.; atr Thomas Jefferson University , Department of
Pathology, Anatomy, and Cell Biology , Sydney Kimmel Medical College , Philadelphia
, PA , USA.; ua Instituto Oswaldo Cruz, FIOCRUZ, Laboratório de Biologia Celular ,
Rio de Janeiro , Brazil.; oq Hannover Medical School , Department of Biochemistry ,
Hannover , Germany.; di Center of Investigation and Advanced Studies, Cinvestav-IPN
, Mexico City , Mexico.; uf IRCCS Casa Sollievo della Sofferenza, Medical Genetics
Unit , San Giovanni Rotondo (FG) , Italy.; uy Istituto Ortopedico Rizzoli IOR-
IRCCS, Laboratory of Musculoskeletal Cell Biology , Bologna , Italy.; bvi
University of Sydney , Department f Pathology and Bosch Institute , Sydney, New
South Wales , Australia.; bla University of Innsbruck, Institute for Biomedical
Aging Research , Innsbruck , Austria.; vf Italian National Institute of Health ,
Department of Technology and Health, Rome , Italy.; jl Duke University, Nicholas
School of the Environment , Durham , NC , USA.; atn Third Military Medical
University, Research Center for Nutrition and Food Safety, Institute of Military
Preventive Medicine , Chongqing , China.; ani Second Military Medical University ,
Department of Pharmacology , Shanghai , China.; uf IRCCS Casa Sollievo della
Sofferenza, Medical Genetics Unit , San Giovanni Rotondo (FG) , Italy.; bt
Biochimie et Physiologie Moléculaire des Plantes, UMR5004 CNRS/INRA/UM2/SupAgro,
Institut de Biologie Intégrative des Plantes , Montpellier , France.; bpe
University of Namur, Laboratory of Biochemistry and Cell Biology (URBC), Namur
Research Institute for Life Sciences (NARILIS) , Namur , Belgium.; adc Icahn School
of Medicine at Mount Sinai , Department of Medicine , New York , NY , USA.; alr
Royal North Shore Hospital, Cardiovascular and Hormonal Research Laboratory, Royal
North Shore Hospital and Kolling Institute, Sydney , NSW , Australia.; bvg
University of Sydney , Department of Cardiology , Sydney, NSW , Australia.; acx
Monash University, Clayton Campus , Department of Biochemistry and Molecular
Biology , Melbourne , Victoria , Australia.; alj RIKEN Brain Science Institute,
Laboratory for Developmental Neurobiology , Saitama , Japan.; axw Università Vita-
Salute San Raffaele , Milan , Italy.; caj San Raffaele Scientific Institute , Milan
, Italy.; bgp University of Cambridge , Department of BIochemistry , Cambridge ,
UK.; bwe University of Texas, MD Anderson Cancer Center , Department of Systems
Biology , Houston , TX , USA.; ajg University of Oslo and Oslo University Hospital,
Prostate Cancer Research Group, Centre for Molecular Medicine (Norway) , Oslo ,
Norway.; bqn University of Oslo , Department of Molecular Oncology , Department of
Urology , Oslo , Norway.; bqq Centre for Cancer Research and Cell Biology, Queen's
University Belfast, Lisburn Road, Belfast, UK.; bax University Hospital Erlangen,
Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) , Erlangen , Germany.; asw
The Hospital for Sick Children , Department of Paediatrics , Toronto, Ontario ,
Canada.; bjt University of Fribourg , Department of Medicine , Division of
Physiology , Fribourg , Switzerland.; aly Russian Academy of Sciences, Kazan
Institute of Biochemistry and Biophysics , Kazan, Tatarstan , Russia.; aqw Swedish
University of Agricultural Sciences and Linnean Center for Plant Biology,
Department of Chemistry and Biotechnology, Uppsala BioCenter, Uppsala, Sweden.; bs
Bio21 Molecular Science and Biotechnology Institute , Department of Biochemistry
and Molecular Biology , Parkville , Victoria , Australia.; bol University of
Milan , Department of Experimental Oncology , European Institute of Oncology and
Department of Biosciences , Milan , Italy.; awx Instituto de Biomedicina de
Sevilla, Hospital Universitario Virgen del Rocío, Consejo Superior de
Investigaciones Científicas, Universidad de Sevilla , Sevilla , Spain.; brn
University of Pennsylvania , Department of Anatomy and Cell Biology ,
Philadelphia , PA , USA.; bfs University of California San Diego , Department of
Pharmacology , La Jolla , CA , USA.; auo Tokyo Medical University , Department of
Biochemistry , Tokyo , Japan.; bwv University of Tokyo , Department of Biochemistry
and Molecular Biology , Graduate School and Faculty of Medicine , Tokyo , Japan.;
agp National University of Ireland, Apoptosis Research Centre , Galway , Ireland.;
rv INSERM U1081, CNRS UMR7284, Institute of Research on Cancer and Ageing of Nice
(IRCAN) , Nice , France.; zo Linköping University , Department of Clinical and
Experimental Medicine , Linköping , Sweden.; tu Instituto Gulbenkian de Ciência ,
Oeiras , Portugal.; uk IRCCS Santa Lucia Foundation , Rome , Italy.; jt Institute
for Research in Biomedicine, Bellinzona, Switzerland Università Svizzera italiana,
Lugano , Switzerland.; ju Ecole Polytechnique Federale de Lausanne, Global Health
Institute, School of Life Sciences , Lausanne , Switzerland.; g Aarhus University,
Medical Research Laboratory, Institute for Clinical Medicine , Aarhus , Denmark.;
ayx Université de Lyon, UMR 5239 CNRS, Laboratory of Molecular Biology of the Cell,
Ecole Normale Supérieure de Lyon , Lyon , France.; hy Consejo Superior de
Investigaciones Científicas (CSIC), Universidad de Salamanca, Campus Miguel de
Unamuno, Instituto de Biología Molecular y Celular del Cáncer, Centro de
Investigación del Cáncer , Salamanca , Spain.; bre University of Padova, Venetian
Institute of Molecular Medicine , Department of Biomedical Science , Padova ,
Italy.; blf University of Iowa , Department of Medicine , Iowa City , IA , USA.;
bpg University of Naples Federico II , Department of Veterinary Medicine and
Animal Production , Naples , Italy.; ahe Neuroscience Research Institute , Santa
Barbara , CA , USA.; bgk University of California Santa Barbara , Department of
Molecular , Cellular, and Developmental Biology , Santa Barbara , CA , USA.; bzn
Van Andel Institute, Center for Neurodegenerative Science , Grand Rapids , MI ,
USA.; bjd University of Exeter Medical School, European Centre for Environment and
Human Health (ECEHH), Truro , Cornwall , UK.; awp Universidad de Costa Rica, CIET ,
San José , Costa Rica.; bho University of Coimbra, CNC - Center for Neuroscience
and Cell Biology and Faculty of Medicine , Coimbra , Portugal.; azw Université
Paris Descartes-Sorbonne Paris Cité, Institut Necker Enfants-Malades (INEM), INSERM
U1151-CNRS UMR 8253 , Paris , France.; anc Sapienza University of Rome , Department
of Molecular Medicine , Rome , Italy.; bzf University Roma Tre , Department of
Science , LIME, Rome , Italy.; bih University of Colorado, School of Medicine ,
Department of Pharmacology , Aurora , CO , USA.; aps Sorbonne Universités, UPMC
Univ Paris 06, INSERM U1135, CNRS ERL 8255, Center for Immunology and Microbial
Infections - CIMI-Paris , Paris , France.; aml Saitama University, Graduate School
of Science and Engineering , Saitama , Japan.; buh University of South Australia,
Early Origins of Adult Health Research Group, School of Pharmacy and Medical
Sciences, Sansom Institute for Health Research, Adelaide , SA , Australia.; amj
Saint Louis University School of Medicine , Department of Molecular Microbiology
and Immunology , St. Louis , MO , USA.; akj Pontificia Universidad Católica de
Chile , Physiology Department , Santiago , Chile.; amq Sanford Burnham Prebys NCI-
Cancer Center, Cell Death and Survival Networks Program , La Jolla , CA , USA.; bpl
University of New Mexico , Department of Internal Medicine , Albuquerque , NM ,
USA.; rc Imperial College London, Section of Microbiology, MRC Centre for Molecular
Bacteriology and Infection , London , UK.; aay Max Planck Institute for Biology of
Ageing , Cologne , Germany.; bme University of Liege , GIGA-Signal Transduction
Department , Protein Signalisation and Interaction Laboratory , Liège , Belgium.;
byw University of York, Centre for Immunology and Infection , Department of Biology
, Hull York Medical School , York , UK.; mv Georgetown University Medical Center ,
Department of Neuroscience , Washington, DC , USA.; bcw University of Athens,
Medical School , Second Department of Internal Medicine and Research Institute ,
Attikon University General Hospital , Athens , Greece.; byp University of Wisconsin
, Department of Dermatology , Madison , WI , USA.; bic University of Colorado ,
Department of Pediatrics , Center for Cancer and Blood Disorders , Aurora , CO ,
USA.; hc CNRS, Immunopathology and Therapeutic Chemistry, Institut de Biologie
Moléculaire et Cellulaire , Strasbourg , France.; tx Instituto Nacional de
Investigación y Tecnología Agraria y Alimentaria (INIA) , Departamento de
Biotecnología , Madrid , Spain.; bl Bellvitge Biomedical Research Institute
(IDIBELL), L'Hospitalet, Cell Death Regulation Group , Barcelona , Spain.; bzb
University of Zurich, Ins itute of Experimental Immunology , Zurich , Switzerland.;
atj The Wistar Institute, Program in Molecular and Cellular Oncogenesis ,
Philadelphia , PA , USA.; auz Trinity College Dublin, School of Biochemistry and
Immunology, Trinity Biomedical Sciences Institute , Dublin , Ireland.; mj Genentech
Inc. , Department of Cancer Immunology , South San Francisco , CA , USA.; cbe
Washington University, School of Medicine, Departments of Obstetrics and
Gynecology, and Pathology and Immunology , St. Louis , MO , USA.; bee University of
British Columbia , Department of Cellular and Physiological Sciences , Vancouver,
BC , Canada.; bgu University of Camerino, School of Pharmacy , Camerino , Italy.;
xj Karolinska Institute , Department of Physiology and Pharmacology , Stockholm ,
Sweden.; aui Tokyo Denki University, Division of Life Science and Engineering ,
Hatoyama, Hiki-gun, Saitama , Japan.; aei National Center of Neurology and
Psychiatry , Department of Degenerative Neurological Diseases , Kodaira, Tokyo ,
Japan.; yu Kyoto Sangyo University , Department of Life Sciences , Kyoto , Japan.;
ale Radboud University, Institute for Molecules and Materials , Department of
Molecular Materials , Nijmegen , The Netherlands.; kj Eötvös Loránd University ,
Department of Anatomy , Cell and Developmental Biology , Budapest , Hungary.; rk
Indiana University School of Medicine , Department of Dermatology , Indianapolis ,
IN , USA.; byv University of Wyoming, School of Pharmacy, College of Health
Sciences , Laramie , WY , USA.; atx Toho University, School of Medicine ,
Department of Biochemistry , Tokyo , Japan.; auk Tokyo Institute of Technology,
Graduate School of Bioscience and Biotechnology , Tokyo , Japan.; buq University of
South Florida , Department of Cell Biology , Microbiology, and Molecular Biology ,
Tampa , FL , USA.; asa Telethon Institute of Genetics and Medicine (TIGEM) ,
Pozzuoli, Naples , Italy.; asb Temasek Life Sciences Laboratory , Singapore.; aez
National Institute for Infectious Diseases "L. Spallanzani" IRCCS , Rome , Italy.;
pd Harvard Medical School, Neurology Residency Program, Brigham and Women's
Hospital and Massachusetts General Hospital , Boston , MA , USA.; bgo University of
Cambridge, Cancer Research UK Cambridge Institute, Li Ka Shing Centre , Cambridge ,
UK.; azw Université Paris Descartes-Sorbonne Paris Cité, Institut Necker Enfants-
Malades (INEM), INSERM U1151-CNRS UMR 8253 , Paris , France.; bzy Virginia
Commonwealth University , Department of Internal Medicine , Division of Pulmonary
Disease and Critical Care Medicine , Richmond , VA , USA.; btt University of São
Paulo, Ribeirão Preto Medical School , Department of Physiology , Ribeirão Preto,
São Paulo , Brazil.; bvv University of Texas, Health Science Center at San Antonio,
CTRC Institute for Drug Development , San Antonio , TX , USA.; bfv University of
California San Diego, Division of Biological Sciences, Section of Molecular Biology
, La Jolla, CA , USA.; bkw University of Illinois at Chicago, Deprtment of
Biochemistry and Molecular Genetics , Chicago , IL , USA.; atq Thomas Jefferson
University , Department of Pathology , Anatomy and Cell Biology , Philadelphia , PA
, USA.; bjg University of Ferrara , Department of Morphology , Surgery and
Experimental Medicine , Ferrara , Italy.; alc Radboud University Nijmegen Medical
Center , Department of Internal Medicine , Nijmegen , The Netherlands.; alb Radboud
University Nijmegen Medical Center , Department of Internal Medicine , Division of
Endocrinology , Nijmegen , The Netherlands.; bcz University of Aveiro/QOPNA ,
Department of Chemistry , Aveiro , Portugal.; ahg New York Blood Center, Lindsley
F. Kimball Research Institute , New York , NY , USA.; byl University of Warwick,
Life Sciences , Coventry , UK.; ayn Université d'Auvergne, M2iSH "Microbes,
Intestine, Inflammation, Susceptibility of the Host", UMR 1071 INSERM, Centre
Biomédical de Recherche et Valorisation, Faculté de Médecine , Clermont-Ferrand ,
France.; bxo University of Tübingen, Institute of Medical Genetics and Applied
Genomics , Tübingen , Germany.; sg INSERM U964, CNRS UMR7104, Université de
Strasbourg , Department of Translational Medecine , Institut de Génétique et de
Biologie Moléculaire et Cellulaire (IGBMC) , Illkirch , France.; s Akershus
University Hospital , Oslo , Norway.; bqo University of Oslo , Department of
Clinical Molecular Biology , Oslo , Norway.; xh Karolinska Institute, Center for
Alzheimer Research , Department of Neurobiology , Care Sciences and Society,
Division for Neurogeriatrics , Huddinge , Sweden.; zh Laboratory for Proteolytic
Neuroscience, RIKEN Brain Science Institute, Wako , Saitama , Japan.; aex National
Institute for Basic Biology , Department of Cell Biology , Okazaki , Japan.; aej
National Center of Neurology and Psychiatry , Department of Neuromuscular
Research , National Institute of Neuroscience , Tokyo , Japan.; awq Universidad de
Genética , Facultad de Enfermería y Terapia Ocupacional , Cáceres , Spain.; apl
Soochow University , Department of Pathogenic Biology , Suzhou, Jiangsu , China.;
aho New York University, Nathan Kline Institute , Orangeburg , NY , USA.; bnh
University of Maryland, School of Medicine , Department of Chemistry , Baltimore ,
MD , USA.; aiy Osaka University, Graduate School of Dentistry , Osaka , Japan.; bhw
University of Cologne, Institute of Biochemistry I, Medical Faculty , Koeln ,
Germany.; bst University of Pretoria , Department of Physiology , Pretoria, Gauteng
, South Africa.; xj Karolinska Institute , Department of Physiology and
Pharmacology , Stockholm , Sweden.; bcl University of Antwerp , Department of
Paediatric Oncology , Antwerp , Belgium.; bly Hakim Sabzevari University,
Department of Biology, Faculty of Basic Sciences, Sabzevar, Iran.; pe Harvard
Medical School, Ophthalmology , Boston , MA , USA.; bjj University of Florida,
College of Medicine , Department of Neuroscience , Gainesville , FL , USA.; aco
Medical University of Vienna, Internal Medicine I , Vienna , Austria.; wo Juntendo
University, Graduate School of Medicine , Department of Neuroscience for
Neurodegenerative Disorders , Tokyo , Japan.; bxn University of Tübingen, Center
for Plant Molecular Biology (ZMBP) , Department of Plant Biochemistry , Tübingen ,
Germany.; cu Cardiff University, Systems Immunity Research Institute , Cardiff ,
Wales, UK.; ban University College Cork, Cork Cancer Research Centre, BioSciences
Institute, Co. Cork , Ireland.; brl University
of Pennsylvania, Abramson Cancer Center , Philadelphia , PA , USA.; nh German
Cancer Research Center (DKFZ), Clinical Cooperation Unit (CCU) Pediatric Oncology ,
Heidelberg , Germany.; hv Consejo Superior de Investigaciones Científicas (CSIC),
Centro de Investigaciones Biológicas , Madrid , Spain.; afd National Institute of
Infectious Diseases , Department of Bacteriology I , Tokyo , Japan.; aci Medical
University of South Carolina , Department of Biochemistry and Molecular Biology ,
Hollings Cancer Center , Charleston , SC , USA.; aqh St. Marianna University School
of Medicine , Department of Physiology , Kanagawa , Japan.; cci Yonsei University,
College of Life Science and Biotechnology , Department of Systems Biology , Seoul ,
Korea.; yp Kumamoto University, Institute of Resource Development and Analysis ,
Kumamoto , Japan.; aug Tokushima Bunri University, Faculty of Pharmaceutical
Sciences at Kagawa Campus, Sanuki City , Kagawa , Japan.; akl Post Graduate
Institute of Medical Education and Research (PGIMER) , Department of Urology ,
Chandigarh , India.; aiz Osaka University, Graduate School of Frontier
Biosciences , Osaka , Japan.; ww Kanazawa Medical University , Department of
Medicine , Ishikawa , Japan.; ts Instituto de Parasitología y Biomedicina López
Neyra (IPBLN), CSIC , Granada , Spain.; zo Linköping University , Department of
Clinical and Experimental Medicine , Linköping , Sweden.; bil University of
Copenhagen , Department of Plant and Environmental Sciences , Sect on for Genetics
and Microbiology , Copenhagen , Denmark.; boc University of Michigan , Department
of Molecular , Cellular, and Developmental Biology , Ann Arbor , MI , USA.; bog
University of Michigan, Life Sciences Institute , Ann Arbor , MI , USA.; bfq
University of California San Diego , Department of Pediatrics , La Jolla , CA ,
USA.; azw Université Paris Descartes-Sorbonne Paris Cité, Institut Necker Enfants-
Malades (INEM), INSERM U1151-CNRS UMR 8253 , Paris , France.; bvj University of
Szeged , Department of Medical Microbiology and Immunobiology , Szeged, Csongrád ,
Hungary.; bws University of Virginia, Departments of Biology and Cell Biology,
Charlottesville, VA, USA.; byc University of Valencia , Departamento de Bioquimica
y Biologia Molecular , IATA-CSIC , Valencia , Spain.; byd University of Valencia ,
Departamento de Biotecnología , IATA-CSIC , Valencia , Spain.; byf University of
Valencia , Department of Physiology , Burjassot, Valencia , Spain.; uz Istituto
Superiore di Sanità , Department of Cell Biology and Neurosciences , Rome , Italy.;
acx Monash University, Clayton Campus , Department of Biochemistry and Molecular
Biology , Melbourne , Victoria , Australia.; bym University of Washington ,
Department of Pathology , Seattle , WA.; aum Tokyo Medical and Dental University ,
Department of Gastroenterology and Hepatology , Tokyo , Japan.; nt Goethe
University, Institute for Molecular Biosciences, Molecular Developmental Biology ,
Frankfurt , Hesse , Germany.; aiv Osaka University Graduate School of Medicine ,
Department of Pediatrics , Osaka , Japan.; xt King's College London, Cardiovascular
Division , London , UK.; bux University of Southern California, Keck School of
Medicine , Department of Molecular Microbiology and Immunology , Los Angeles , CA ,
USA.; bbr University Medical Centre Göttingen , Department of Neurodegeneration and
Restorative Research , Göttingen , Germany.; vw Jinan University , Department of
Immunobiology , College of Life Science and Technology , Guangzhou , China.; bsf
University of Pittsburgh, School of Dental Medicine , Department of Endodontics ,
Pittsburgh , PA , USA.; acr Memorial Sloan Kettering Cancer Center , New York ,
NY , USA.; bpk University of New Mexico, Comprehensive Cancer Center , Department
of Molecular Genetics and Microbiology , Albuquerque , NM , USA.; aib Northwestern
University , Department of Neurology , Feinberg School of Medicine , Chicago , IL ,
USA.; avy University of Texas, MD Anderson Cancer Center , Department of
Experimental Therapeutics , Houston , TX , USA.; azc Université de Montréal ,
Department of Pharmacology , Faculty of Medicine , Montreal, QC , Canada.; zg
Laboratory for Biomedical Neurosciences NSI/EOC, Neurodegeneration Group ,
Torricella-Taverne , Switzerland.; bso University of Poitiers, EA3808 Molecular
Targets and Therapeutics in Alzheimer's Disease , Poitiers , France.; rx University
of Nice Sophia Antipolis, Institute of Research on Cancer and Ageing of Nice, CNRS
UMR 7284/INSERM U1081 , Nice , France.; bpg University of Naples Federico II ,
Department of Veterinary Medicine and Animal Production , Naples , Italy.; adb
Mossakowski Medical Research Centre, Polish Academy of Sciences, Electron
Microscopy Platform , Warsaw , Poland.; cat Warsaw University of Life Sciences -
SGGW, Faculty of Veterinary Medicine , Department of Physiological Sciences ,
Warsaw , Poland.; bsk University of Pittsburgh, School of Medicine , Department of
Pediatrics , Pittsburgh , PA , USA.; agp National University of Ireland, Apoptosis
Research Centre , Galway , Ireland.; bez University of California Davis ,
Department of Medical Microbiology and Immunology , School of Medicine , Davis , CA
, USA.; er Charles University in Prague, Faculty of Science , Department of
Genetics and Microbiology , Prague , Czech Republic.; azo Université Lyon, Ecole
Normale Supérieure de Lyon , Lyon , France.; bls University of Kiel, Institute of
Human Nutrition and Food Science , Kiel , Germany.; sa INSERM U1147 , Paris ,
France.; byg University of Verona , Department of eurological, Biomedical and
Movement Sciences , Verona , Italy.; d Aarhus University, Department of Biomedicine
, Aarhus , Denmark.; btg University of Rome "Tor Vergata" , Department of Clinical
Sciences and Translational Medicine , Rome , Italy.; bri University of Pavia ,
Department of Biology and Biotechnology , Pavia , Italy.; z Albert Einstein College
of Medicine , Department of Developmental and Molecular Biology , Institute for
Aging Studies , Bronx , NY , USA.; cdb Zhejiang University, Sir Run Run Shaw
Hospital , Department of Medical Oncology , Hangzhou, Zhejiang , China.; agl
Department of Cardiology, Nanhai Hospital Affiliated to Southern Medical
University, Foshan, Guangdong Province, China.; xg Karolinska Institute, Cancer
Center Karolinska , Department of Oncology-Pathology , Stockholm , Sweden.; asj
Texas A&M University , Department of Microbial Pathogenesis and Immunology , Texas
A&M Health Science Center , Bryan , TX , USA.; asl Texas A&M University, The Norman
Borlaug Center , College Station , TX , USA.; ec Centro de Investigaciones
sy Institute for Clinical and Experimental Medicine, Centre for Experimental
Medicine , Department of Metabolism and Diabetes , Prague , Czech Republic.; awf
Universidad de Buenos Aires, Inmunología, Facultad de Farmacia y Bioquímica ,
Buenos Aires , Argentina.; aee National and Kapodistrian University of Athens ,
Department of Cell Biology and Biophysics , Faculty of Biology , Athens , Greece.;
bjh University of Florence , Department of Biology , Florence , Italy.; bct
University of Arkansas for Medical Sciences , Department of Pharmacology/Toxicology
, Little Rock , AR , USA.; axb IIS Aragon, Universidad de Zaragoza/Araid, Centro de
Investigación Biomédica de Aragón , Zaragoza , Spain.; bzu Vanderbilt University,
School of Medicine, Pathology Microbiology and Immunology , Nashville , TN , USA.;
ld Federico II University, Telethon Institute of Genetics and Medicine (TIGEM) ,
Department of Medical and Translational Sciences , Naples , Italy.; abz Medical
College of Wisconsin , Department of Biochemistry , Milwaukee , WI , USA.; ccm
Yonsei University, Division of Biological Science and Technology , Wonju , Korea.;
yh Korea University, Division of Life Sciences , Seoul , Korea.; bid University of
Colorado, HHMI , Department of Chemistry and Biochemistry , Aurora , CO , USA.; bkh
University of Heidelberg, Institute of Anatomy and Cell Biology , Heidelberg ,
Germany.; bxv University of Ulm, Institute of Applied Physiology , Ulm , Germany.;
yo KU Leuven, Laboratory of Molecular and Cellular Signaling , Department of
Cellular and Molecular Medicine , Leuven , Belgium.; boc University of Michigan ,
Department of Molecular , Cellular, and Developmental Biology , Ann Arbor , MI ,
USA.; bog University of Michigan, Life Sciences Institute , Ann Arbor , MI , USA.;
ayf Université Bordeaux Segalen, U1035 INSERM, Hématopoïèse Leucémique et Cibles
Thérapeutiques , Bordeaux , France.; amv Sanofi , Vitry Sur Seine , France.; il
Dalhousie University , Department of Pharmacology , Halifax, Nova Scotia , Canada.;
bbc University Hospital of Göttingen , Department of Nephrology and Rheumatology ,
Göttingen , Germany.; ahr New York-Presbyterian Hospital/Weill-Cornell Medical
Center , New York , NY , USA.; us IRCM, Institut de Recherche en Cancérologie de
Montpellier , Montpellier , France.; ayz Université de Montpellier, Institut
régional du Cancer de Montpellier, INSERM, U 1194 , Montpellier , France.; bum
University of South Dakota, Division of Basic Biomedical Sciences , Vermillion , SD
, USA.; abn McGill University , Goodman Cancer Research Centre and Department of
Biochemistry , Montreal, Quebec , Canada.; bbb University Hospital Muenster Albert-
Schweitzer-Campus, Internal Medicine D , Department of Nephrology , Hypertension
and Rheumatology , Münster , Germany.; ha CNR, Institute of Cell Biology and
Neurobiology and IRCCS Santa Lucia Foundation , Rome , Italy.; bhg P Catholic
University of Ch le, Advanced Center for Chronic Diseases (ACCDiS), Faculty of
Medicine , Santiago , Chile.; bjf University of Extremadura , Department of
Medicine , Faculty of Veterinary Medicine , Cáceres , Spain.; ec Centro de
Investigaciones Biológicas
(CSIC) , Department of Cellular and Molecular Biology , Madrid , Spain.; azt
Université Paris Descartes, Institut Necker-Enfants Malades, INSERM, U1151 ,
Paris , France.; dj College of Science, Central China Normal University , Wuhan ,
China.; bxq University of Turin , Department of Clinical and Biological Sciences ,
Unit of Experimental Medicine and Clinical Pathology , Turin , Italy.; tj Institute
of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) , Vienna ,
Austria.; dg Center for Dementia Research, Nathan S. Kline Institute , Orangeburg ,
NY , USA.; bnt Murdoch Childrens Research Institute, University of Melbourne ,
Department of Paediatrics , Royal Children's Hospital , Melbourne , Victoria ,
Australia.; lb Federal University of São Paulo , Department of Pharmacology ,
Paulista School of Medicine , São Paulo , Brazil.; bht University of Coimbra,
IBILI, Faculty of Medicine , Coimbra , Portugal.; ty Instituto Nacional de
Neurología y Neurocirugía, Neurochemistry Unit , Mexico City , Mexico.; awz
Universidad de Sevilla, Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC ,
Sevilla , Spain.; awr Universidad de León, Área de Biología Celular, Instituto de
Biomedicina , León , Spain.; hv Consejo Superior de Investigaciones Científicas
(CSIC), Centro de Investigaciones Biológicas , Madrid , Spain.; bzl Vall d'Hebron
Research Institute, Neurodegenerative Diseases Lab , Barcelona , Spain.; aqp State
University of New York, College of Medicine , Departments of Medicine ,
Microbiology and Immunology, Biochemistry and Molecular Biology , Syracuse , NY ,
USA.; bse University of Pittsburgh , Pittsburgh , PA , USA.; beq University of
Calabria , Department of Biology , Ecology and Earth Science, Laboratory of
Electron Microscopy , Cosenza , Italy.; ig Curtin University, School of Biomedical
Sciences , Perth , Australia.; ago National University Cancer Institute, National
University Health System , Singapore.; agv National University of Singapore ,
Department of Physiology , Yong Loo Lin School of Medicine , Singapore.; bix
University of Eastern Finland, Faculty of Health Science, School of
Pharmacy/Toxicology , Kuopio , Finland.; af Albert Einstein College of Medicine,
Departments of Medicine and Molecular Pharmacology , Bronx , NY , USA.; cak VU
University Medical Center , Department of Medical Oncology , Amsterdam , The
Netherlands.; bik University of Copenhagen , Department of Biology , Copenhagen ,
Denmark.; afy National Jewish Health , Denver , CO , USA.; abo McGill University,
Health Centre Research Institute, Meakins Christie Laboratories , Montreal,
Quebec , Canada.; aja Oslo University Hospital, Center for Eye Research , Oslo ,
Norway.; bqp University of Oslo , Department of Ophthalmology , Oslo , Norway.; bvk
University of Szeged , Department of Ophthalmology , Faculty of Medicine , Szeged ,
Hungary.; aha NCI/CCR, Basic Research Laboratory , Frederick , MD , USA.; btd
University of Rome "Tor Vergata" , Department of Biology , Rome , Italy.; uz
Istituto Superiore di Sanità , Department of Cell Biology and Neurosciences ,
Rome , Italy.; p Aix-Marseille Université, U2M, Centre d'Immunologie de Marseille-
Luminy , Marseille , France.; hd CNRS, UMR 7280 , Marseille , France.; sl INSERM,
U1104 , Marseille , France.; bcy University of Aveiro, Institute for Research in
Biomedicine - iBiMED, Aveiro Health Sciences Program , Aveiro , Portugal.; azq
Université Nice Sophia Antipolis, UMR E-4320TIRO-MATOs CEA/iBEB, Faculté de
Médecine , Nice , France.; km Centre de Recherche des Cordeliers, Equipe 11
labellisée par la Ligue Nationale contre le Cancer , Paris , France.; oc Gustave
Roussy Cancer Campus , Villejuif , France.; sn INSERM, U1138 , Paris , France.; azv
Université Paris Descartes/Paris V , Paris , France.; aww Universidad de Salamanca,
Campus Unamuno Instituto de Biologia Molecular y Celular del Cancer (IBMCC), Centro
de Investigacion del Cancer , Salamanca , Spain.; ec Centro de Investigaciones
tz Instituto Nacional de Neurología y Neurocirugía, Neuroimmunology and Neuro-
Oncology Unit , Mexico City , Mexico.; arw Tel Aviv University , Department of
Neurobiology , Tel-Aviv , Israel.; bow University of Modena and Reggio Emilia ,
Department of Life Sciences , Modena , Italy.; bjg University of Ferrara ,
Department of Morphology , Surgery and Experimental Medicine , Ferrara , Italy.; ah
Albert Einstein College of Medicine, Montefiore Medical Center , Bronx , NY , USA.;
bel University of British Columbia, Michael Smith Laboratories , Department of
Chemical and Biological Engineering , Vancouver, BC , Canada.; vl Jesse Brown VA
Medical Center , Department of Medicine , Chicago , IL , USA.; aie Northwestern
University, Robert H. Lurie Comprehensive Cancer Center , Chicago , IL , USA.; als
Ruhr University Bochum, Biochemie Intrazellulärer Transportprozesse , Bochum ,
Germany.; aql Stanford University, School of Medicine , Department of Pathology ,
Stanford , CA , USA.; mp Georg-August-Universität Göttingen, Institute of
Microbiology and Genetics , Department of Genetics of Eukaryotic Microorganisms ,
Göttingen , Germany.; bxh University of Toulouse, INSERM UMR 1037, Cancer Research
Center of Toulouse , Toulouse , France.; hq Comenius University in Bratislava ,
Department of Biochemistry , Faculty of Natural Sciences , Bratislava , Slovak
Republic.; axq Universita' degli Studi di Milano , Dipartimento di Scienze
Farmacologiche e Biomolecolari , Milan , Italy.; abq McGill University, Health
Centre Research Institute, Meakins-Christie Laboratories , Montreal, Quebec,
Canada.; bog University of Michigan, Life Sciences Institute , Ann Arbor , MI ,
USA.; mo Georg-August-Universität Göttingen , Department of Molecular Microbiology
and Genetics , Institute of Microbiology and Genetics , Göttingen , Germany.; bue
University of Silesia , Department of Animal Histology and Embryology , Katowice ,
Poland.; bzi USDA-Human Nutrition Research Center on Aging at Tufts University ,
Department of Neuroscience and Aging , Boston , MA , USA.; bra University of Oxford
, Nuffield Department of Obstetrics and Gynaecology , Oxford , UK.; bjm University
of Florida , Department of Applied Physiology and Kinesiology , Gainesville , FL ,
USA.; bfa University of California Davis , Department of Molecular and Cellular
Biology , Davis , CA , USA.; dw Centro Andaluz de Biología Molecular y Medicina
Regenerativa, Consejo Superior de Investigaciones Científicas , Sevilla , Spain.;
bar University College London, MRC Laboratory for Molecular Cell Biology , London ,
UK.; wa Johannes Gutenberg University Mainz, University Medical Center , Department
of Medical Microbiology and Hygiene , Mainz , Germany.; acx Monash University,
Clayton Campus , Department of Biochemistry and Molecular Biology , Melbourne ,
Victoria , Australia.; ayd Université Bordeaux Ségalen, Institut de Biochimie et de
Génétique Cellulaires, CNRS, UMR 5095 , Bordeaux , France.; bgy University of Cape
Town , Department of Human Biology , Cape Town, Western Province , South Africa.;
afv National Institutes of Health, NIDDK, Genetics of Development and Disease
Branch , Bethesda , MD , USA.; js Eberhard Karls University Tübingen, Interfaculty
Institute of Cell Biology , Tübingen , Germany.; aro Technical University Munich,
Institute of Human Genetics , Munich, Bavaria , Germany.; bip University of
Cyprus , Department of Biological Sciences , Bioinformatics Research Laboratory ,
Nicosia , Cyprus.; cbh Wayne State University, School of Medicine, Cardiovascular
Research Institute , Detroit , MI , USA.; afj National Institutes of Health, Cell
Biology and Physiology Center, National Heart, Lung, and Blood Institute , Bethesda
, MD , USA.; bie University of Colorado, School of Medicine, Anschutz Medical
Campus , Aurora , CO , USA.; byr University of W sconsin , Department of Medicine ,
Madison , WI , USA.; bm Bellvitge Biomedical Research Institute (IDIBELL),
Neurometabolic Diseases Laboratory , Barcelona , Spain.; gp CIBERER Spanish Network
for Rare Diseases , Madrid , Spain.; qv ICREA Catalan Institution for Research and
Advanced Studies , Catalonia , Spain.; bav University Hospital Center, University
of Lausanne, Clinic of Neonatology , Department of Pediatrics and Pediatric Surgery
, Lausanne , Switzerland.; blw University of Lausanne , Department of Fundamental
Neurosciences , Faculty of Biology and Medicine , Lausanne , Switzerland.; big
University of Colorado, School of Medicine , Department of Immunology and
Microbiology , Aurora , CO , USA.; cx Case Western Reserve University, School of
Medicine , Department of Physiology and Biophysics , Cleveland , OH , USA.; ccv
Zhejiang University, Institute of Hematology, First Affiliated Hospital, College of
Medicine , Hangzhou , China.; apo Soochow University, School of Pharmaceutical
Science , Department of Pharmacology , Suzhou , China.; aqd St. Jude Children's
Research Hospital , Department of Structural Biology , Memphis , TN , USA.; asb
Temasek Life Sciences Laboratory , Singapore.; byo University of Waterloo ,
Department of Kinesiology , Waterloo, Ontario , Canada.; bjx University of
Georgia , Department of Infectious Diseases , Athens , GA , USA.; afu National
Institutes of Health, NIAMS, Laboratory of Muscle Stem Cells and Gene Regulation ,
Bethesda , MD , USA.; bsa University of Pittsburgh , Department of Pathology ,
Pittsburgh , PA , USA.; qb Hôpital Kirchberg, Laboratoire de Biologie Moléculaire
et Cellulaire du Cancer , Luxembourg.; iu Dartmouth College , Department of
Chemistry , Hanover , NH , USA.; cad Virginia Commonwealth University, Massey
Cancer Center , Department of Internal Medicine , Richmond , VA , USA.; bia
University of Colorado Denver, Skaggs School of Pharmacy and Pharmaceutical
Sciences , Department of Pharmaceutical Sciences
, Aurora , CO , USA.; bbz University of Alabama at Birmingham , Department of Cell
, Developmental, and Integrative Biology (CDIB), Comprehensive Diabetes Center
(UCDC) , Birmingham , AL , USA.; bqi University of Oklahoma Health Sciences
Center , Department of Pathology , Oklahoma City , OK , USA.; bam University
Clinics, Institute of Cellular and Molecular Anatomy (Anatomie 3) , Frankfurt ,
Germany.; bvn University of Tasmania, School of Health Sciences , Launceston ,
Tasmania.; adk MRC Laboratory of Molecular Biology , Cambridge , UK.; bgr
University of Cambridge , Department of Medicine , Addenbrooke's Hospital ,
Cambridge , UK.; bvw University of Texas, Health Science Center at San Antonio ,
Department of Molecular Medicine , San Antonio , TX , USA.; avh U.S. Food and Drug
Administration, Center for Drug Evaluation and Research , Silver Spring , MD ,
USA.; bwi University of Texas, Medical Branch , Department of Nutrition and
Metabolism , Galveston , TX , USA.; pj Heidelberg University, Deutsches
Krebsforschungszentrum, Proteostasis in Neurodegenerative Disease (B180), CHS
Research Group at CellNetworks , Heidelberg , Germany.; wd Johns Hopkins
University, School of Medicine , Baltimore , MD , USA.; pz Hôpital Beaujon ,
Paris , France.; so INSERM, U970 , Paris , France.; ajl Paris Cardiovascular
Research Center - PARCC , Clichy , France.; azu Université Paris Descartes ,
Paris , France.; buj University of South Carolina School of Medicine , Department
of Pathology , Microbiology, and Immunology , Columbia , SC , USA.; cba Washington
University, School of Medicine, Cardiovascular Division , Department of Medicine ,
St. Louis , MO , USA.; cbd Washington University, School of Medicine , Department
of Pathology and Immunology , St. Louis , MO , USA.; byn University of Waterloo ,
Department of Biology , Waterloo, Ontario , Canada.; bbp University Medical Center
Utrecht , Department of Cell Biology , Groningen , The Netherlands.; alp Royal
College of Surgeons in Ireland , Department of Physiology and Medical Physics ,
Dublin , Ireland.; ayc Universitätsklinikum Düss ldorf, Institute for Biochemistry
and Molecular Biology I , Düsseldorf , Germany.; abc Max Planck Institute of
Psychiatry, Translational Research in Psychiatry , Munich , Germany.; ask Texas A&M
University, Texas A&M Health Science Center, College of Medicine, Institute of
Biosciences and Technology , Houston , TX , USA.; m Academic Medical Center,
University of Amsterdam , Department of Cell Biology and Histology , Amsterdam ,
The Netherlands.; byv University of Wyoming, School of Pharmacy, College of Health
Sciences , Laramie , WY , USA.; ajx Pennsylvania State University, College of
Medicine , Department of Pharmacology , Hershey , PA , USA.; bgq University of
Cambridge , Department of Medical Genetics , Cambridge Institute for Medical
Research , Cambridge , UK.; iz Denver VAMC , Denver , CO , USA.; bif University of
Colorado, School of Medicine , Aurora , CO , USA.; axp Universidal de Salamanca,
Campus Miguel de Unamuno , Departamento de Microbiologia y Genetica , Salamanca ,
Spain.; byu University of Wisconsin, School of Veterinary Medicine , Department of
Pathobiological Sciences , Madison , WI , USA.; aqf St. Louis University School of
Medicine , Department of Biochemistry and Molecular Biology , St. Louis , MO ,
USA.; bby University of Adelaide , Department of Genetics and Evolution , School of
Biological Sciences , Adelaide , SA , Australia.; bvi University of Sydney ,
Department of Pathology and Bosch Institute , Sydney, New South Wales , Australia.;
aps Sorbonne Universités, UPMC Univ Paris 06, INSERM U1135, CNRS ERL 8255, Center
for Immunology and Microbial Infections - CIMI-Paris , Paris , France.; bcp
University of Arizona , Department of Entomology , Tucson , AZ , USA.; zw Lorraine
University, CITHéFOR EA3452, Faculté de Pharmacie , Nancy , France.; ail Ohio State
University , Department of Veterinary Biosciences , College of Veterinary
Medicine , Columbus , OH , USA.; amu Sangamo Biosciences , Richmond , CA , USA.;
bls University of Kiel, Institute of Human Nutrition and Food Science , Kiel ,
Germany.; axv Università Politecnica delle Marche , Department of Clinical and
Molecular Sciences , Ancona , Italy.; iw Democritus University of Thrace,
Laboratory of Molecular Hematology , Alexandroupolis , Greece.; brg University of
Parma , Department of Biomedical , Biotechnological and Translational Sciences ,
Parma , Italy.; axm Universidade Federal de Minas Gerais, UFMG , Departamento de
Morfologia , Instituto de Ciências Biológicas , Belo Horizonte , Minas Gerais ,
Brazil.; rj Indiana University School of Medicine , Department of Biochemistry and
Molecular Biology , Indianapolis , IN , USA.; bhm University of Cincinnati,
Cincinnati Children's Hospital , Cincinnati , OH , USA.; bec University of British
Columbia , Department of Biochemistry and Molecular Biology , Vancouver, BC
Canada.; arr Technische Universität Braunschweig, Biozentrum , Braunschweig ,
Germany.; brf University of Palermo , Dipartimento di Scienze e Tecnologie
Biologiche , Chimiche e Farmaceutiche (STEBICEF) , Palermo , Italy.; biu University
of Dundee, Centre for Gene Regulation and Expression, College of Life Sciences ,
UK.; axg Universidade de Lisboa, Research Institute for Medicines (iMed.ULisboa),
Faculty of Pharmacy , Lisboa , Portugal.; ib BioCruces Health Research Institute,
Cruces University Hospital, Stem Cells and Cell Therapy Laboratory , Barakaldo ,
Spain.; ja Department of Cellular and Molecular Medicine , Center for Biological
Research and Center for Biomedical Network Research on Rare Diseases , Madrid ,
Spain.; ec Centro de Investigaciones Biológicas (CSIC) , Department of Cellular and
Molecular Biology , Madrid , Spain.; xa Kansas State University, Division of
Biology , Manhattan , KS , USA.; np Goethe University of Frankfurt, Institute of
Biophysical Chemistry , Frankfurt am Main , Germany.; bz Boise State University ,
Department of Biological Sciences, Boise , ID , USA.; ack Medical University of
South Carolina , Department of Ophthalmology , Charleston , SC , USA.; blb
University of Insubria , Department of Biotec nology and Life Sciences , Varese ,
Italy.; brs University of Perugia , Department of Experimental Medicine , Perugia ,
Italy.; axd Universidad Nacional de Cuyo (FCM-UNCUYO), Instituto de Histologia y
Embriologia (IHEM-CONICET), Facultad de Ciencias Medicas , Mendoza , Argentina.;
awo Universidad de Córdoba, Campus de Excelencia Agroalimentario (ceiA3),
Departamento de Genética , Córdoba , Spain.; aya Universitat de Barcelona,
L'Hospitalet de Llobregat , Departament de Ciències Fisiol∫giques II , Campus de
Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) , Barcelona ,
Spain.; ako Public Health England, Health Protection Services, Modelling and
Economics Unit, Colindale , London , UK.; ii Dalhousie University, Biochemistry and
Molecular Biology , Halifax, NS , Canada.; ik Dalhousie University , Department of
Pediatrics , Halifax, Nova Scotia , Canada.; blr University of Kiel, Institute of
Clinical Molecular Biology , Kiel , Germany.; bue University of Silesia ,
Department of Animal Histology and Embryology , Katowice , Poland.; bcb University
of Alabama at Birmingham , Department of Pathology , Birmingham , AL , USA.; sp
Institut d'Investigacions Biomèdiques August Pi I Sunyer (IDIBAPS) , Hemato-
oncology Department , Barcelona , Spain.; bze University Pierre et Marie Curie,
UMR8256/INSERM U-1164, Biological Adaptation and Ageing (B2A) , Paris , France.;
aag Maastricht University, Maastricht Radiation Oncology (MaastRO) Lab, GROW -
School for Oncology and Developmental Biology , Maastricht , The Netherlands.; bq
Beth Israel Deaconess Medical Center, Medical Genetics , Boston , MA , USA.; awy
Universidad de Sevilla, Instituto de Biomedicina de Sevilla , Sevilla , Spain.; bgm
University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's
Hospital , Department of Medical Genetics , Cambridge , UK.; blm University of
Kentucky , Department of Biology , Lexington , KY , USA.; bn Ben-Gurion
University , Department of Clinical Biochemistry and the National Institute of
Biotechnology in the Negev , Beer-Sheva , Israel.; eq Charles University in Prague,
Faculty of Medicine in Hradec Kralove , Department of Medical Biology and
Genetics , Hradec Kralove , Czech Republic.; aaq Mannheim University of Applied
Sciences, Institute of Molecular and Cell Biology , Mannheim , Germany.; bdf
University of Basel , Biozentrum, Basel, BS , Switzerland.; awo Universidad de
Córdoba, Campus de Excelencia Agroalimentario (ceiA3), Departamento de Genética ,
Córdoba , Spain.; ada Monash University, School of Biological Sciences ,
Melbourne , Victoria , Australia.; axq Universita' degli Studi di Milano ,
Dipartimento di Scienze Farmacologiche e Biomolecolari , Milan , Italy.; aio Ohio
University, Division of Physical Therapy , Athens , OH , USA.; agc National
Research Council, Institute of Food Sciences , Avellino , Italy.; asc Temple
University, Sbarro Institute for Cancer Research and Molecular Medicine, Center for
Biotechnology, College of Science and Technology , Philadelphia , PA , USA.; bes
University of Calabria , Department of Pharmacy , Health and Nutritional Sciences,
Section of Preclinical and Translational Pharmacology , Rende (Cosenza) , Italy.;
aje Oslo University Hospital , Department of Molecular Cell Biology , Institute for
Cancer Research , Oslo , Norway.; bqk University of Oslo, Centre for Cancer
Biomedicine , Oslo , Norway.; tc Institute of Biochemistry and Biophysics , Kazan ,
Russia.; bjy University of Glasgow, Cancer Research UK Beatson Institute ,
Glasgow , UK.; cbn Weill Cornell Medical College , Joan and Sanford I. Weill
Department of Medicine , New York , NY , USA.; cbv Whitehead Institute, HHMI and
Massachusetts Institute
of Technology , Cambridge , MA , USA.; hu Concordia University , Department of
Biology , Montreal , Canada.; abi McGill University , Department of Anatomy and
Cell Biology , Montreal , Canada.; kw European Molecular Biology Laboratory (EMBL),
Structural and Computational Biology Unit , Heidelberg , Germany.; afi National
Institutes of Health, Cardiovascular Bra ch, NHLB , Bethesda , MD , USA.; alz
Rutgers New Jersey Medical School , Department of Cell Biology and Molecular
Medicine , Newark , NJ , USA.; gf Christian Albrechts University, Institut für
Biochemie , Kiel , Germany.; ary Tel Aviv University, Sackler Faculty of Medicine ,
Department of Cell and Developmental Biology , Tel Aviv , Israel.; bvi University
of Sydney , Department of Pathology and Bosch Institute , Sydney, New South Wales ,
Australia.; bvx University of Texas, Health Science Center at San Antonio ,
Department of Pathology , San Antonio , TX , USA.; ahv Nippon Medical School ,
Department of Cardiovascular Medicine , Tokyo , Japan.; auh Tokushima University,
Division of Molecular Genetics, Institute for Enzyme Research , Tokushima , Japan.;
nz Gunma University Graduate School of Medicine , Department of Otolaryngology-Head
and Neck Surgery , Gunma , Japan.; auj Tokyo Institute of Technology, Frontier
Research Center , Yokohama , Japan.; anv Seoul National University , Department of
Plant Science , Seoul , Korea.; apy Spanish National Cancer Research Centre (CNIO),
Cell Division and Cancer Group , Madrid , Spain.; avk UCL Cancer Institute,
Samantha Dickson Brain Cancer Unit , London , UK.; bov University of Minnesota ,
Department of Surgery , Minneapolis , MN , USA.; bg Beckman Research Institute,
City of Hope , Department of Neuroscience , Irell and Manella Graduate School of
Biological Science , Duarte , CA , USA.; va Istituto Superiore di Sanità ,
Department of Haematology , Oncology and Molecular Medicine , Rome , Italy.; agp
National University of Ireland, Apoptosis Research Centre , Galway , Ireland.; brr
Laboratoire Européen Performance Santé Altitude, EA 4604, University of Perpignan
Via Domitia , Font-Romeu , France.; axe Universidad Pablo de Olavide, Centro
Andaluz de Biología del Desarrollo (CABD), Consejo Superior de Investigaciones
Científicas-Junta de Andalucía , Sevilla , Spain.; awi Universidad de Castilla-La
Mancha, Laboratorio de Oncología Molecular, Centro Regional de Investigaciones
Biomédicas , Albacete , Spain.; bre University of Padova, Venetian Institute of
Molecular Medicine , Department of Biomedical Science , Padova , Italy.; acp
MedImmune, Respiratory, Inflammation and Autoimmunity Research Department ,
Gaithersburg , MD , USA.; aax Massachusetts Institute of Technology, Koch Institute
for Integrative Cancer Research , Cambridge , MA , USA.; ag Albert Einstein College
of Medicine, Departments of Pathology, Microbiology and Immunology , New York ,
NY , USA.; bgv University of Camerino, School of Pharmacy, Section of Experimental
Medicine , Camerino, MC , Italy.; qo iBET, Instituto de Biologia Experimental e
Tecnológica , Oeiras , Portugal.; tt Instituto de Tecnologia Química e Biológica
António Xavier, Universidade Nova de Lisboa , Oeiras , Portugal.; vk Jawaharlal
Nehru University, School of Life Sciences , New Delhi , India.; bc Baylor College
of Medicine , Department of Molecular and Human Genetics , Houston , TX , USA.; bxd
University of Toronto, Hospital for Sick Children , Toronto, Ontario , Canada.; dg
Center for Dementia Research, Nathan S. Kline Institute , Orangeburg , NY , USA.;
bds University of Birmingham, Institute of Biomedical Research, Institute of Cancer
and Genomic Sciences, College of Medical and Dental Sciences , Edgbaston,
Birmingham , UK.; ph Health Research Institute Germans Trias i Pujol , Badalona ,
Spain.; bgg University of California San Francisco , Department of Surgery , San
Francisco , CA , USA.; eu Chiba University, Medical Mycology Research Center ,
Chiba , Japan.; wy Kanazawa University Graduate School of Medical Sciences ,
Department of Human Pathology , Kanazawa , Japan.; kj Eötvös Loránd University ,
Department of Anatomy , Cell and Developmental Biology , Budapest , Hungary.; ob
Gunma University, Laboratory of Molecular Traffic, Institute for Moleclualr and
Cellular Regulation , Gunma , Japan.; oa Gunma University, Laboratory of Molecular
Membrane Biology, Institute for Moleclualr and Cellular Regulation , Gunma ,
Japan.; bfn Uni ersity of California San Diego , Department of Medicine , San Diego
, CA , USA.; acu Miami VA Healthcare System and University of Miami Miller School
of Medicine, Oncology/Hematology , Miami , FL , USA.; cp Cambridge University ,
Department of Medicine , Cambridge , UK.; nr Goethe University, Institue of
Pharmacology and Toxicology , Frankfurt am Main , Germany.; alg Research Center
Borstel , Borstel , Germany.; bbg University Hospital Zürich, Division of
Gastroenterology and Hepatolog , Zürich , Switzerland.; bev University of Calgary,
Faculty of Veterinary Medicine , Calgary, AB , Canada.; bex University of
California Berkeley, Howard Hughes Medical Institute , Department of Molecular and
Cell Biology , Berkeley , CA , USA.; cao VU University, Medical Center , Academic
Medical Center, Department of Clinical Genetics and Alzheimer Center , Amsterdam ,
Netherlands.; cap Department of Genome Analysis , Amsterdam , Netherlands.; caq VU
University, Departments of Functional Genomics and Molecular and Cellular
Neuroscience, Center for Neurogenomics and Cognitive Research , Amsterdam ,
Netherlands.; vg IUF-Leibniz Research Institute for Environmental Medicine ,
Duesseldorf , Germany.; bte University of Rome "Tor Vergata" , Department of
Biomedicine and Prevention , Rome , Italy.; vn Jewish General Hospital , Department
of Neurology and Neurosurgery , Department of Medicine , Montreal, Quebec ,
Canada.; abr McGill University, Lady Davis Institute for Medical Research ,
Montreal, Quebec , Canada.; afq National Institutes of Health, National Institute
of Allergy and Infectious Disease, Cytokine Biology Section , Bethesda , MD , USA.;
bbq University Medical Centre Göttingen , Clinic for Neurology and Department of
Neuroimmunology , Göttingen , Germany.; po Systems-oriented Immunology and
Inflammation Research, Helmholtz Centre for Infection Research , Braunschweig ,
Germany.; aji Otto-von-Guericke-University Magdeburg, Institute of Molecular and
Clinical Immunology , Magdeburg , Germany.; alg Research Center Borstel , Borstel ,
Germany.; bbf University Hospital Ulm, Sektion Experimentelle Anaestesiologie , Ulm
, Germany.; z Albert Einstein College of Medicine , Department of Developmental and
Molecular Biology , Institute for Aging Studies , Bronx , NY , USA.; hj Columbia
University Medical Center , New York , NY , USA.; bzc University of Zürich,
Institute of Physiology , Zürich , Switzerland.; buw University of Southern
California , Department of Molecular Microbiology and Immunology , Keck School of
Medicine , Los Angeles , CA , USA.; ut IRO, Institute for Research in Ophthalmology
, Sion , Switzerland.; blx University of Lausanne , Department of Ophthalmology ,
Lausanne , Switzerland.; gf Christian Albrechts University, Institut für
Biochemie , Kiel , Germany.; pk Heidelberg University, Zentrum für Molekulare
Biologie der Universität Heidelberg (ZMBH) , Heidelberg , Germany.; abd Mayo Clinic
, Department of Biochemistry , Rochester , MN , USA.; ln Forschungszentrum Juelich,
ICS-6/Structural Biochemistry , Juelich , Germany.; ca Boston Children's Hospital,
F.M. Kirby Neuroscience Center , Boston , MA , USA.; ug IRCCS Neuromed , Pozzilli,
IS , Italy.; alz Rutgers New Jersey Medical School , Department of Cell Biology and
Molecular Medicine , Newark , NJ , USA.; btc University of Rome "Sapienza" ,
Department of Medical-Surgical Sciences and Biotechnologies , Latina , Italy.; amg
Rutgers University-Robert Wood Johnson Medical School, Rutgers Cancer Institute of
New Jersey , Piscataway , NJ , USA.; tk Institute of Molecular Genetics, National
Research Council , Pavia , Italy.; arg Sunnybrook Research Institute; and
University of Toronto , Department of Biochemistry , Toronto, Ontario , Canada.; pq
Helsinki University , Department of Medical Genetics , Helsinki , Finland.; bsp
University of Porto, Cancer Drug Resistance Group, IPATIMUP - Institute of
Molecular Pathology and Immunology , Porto , Portugal.; bsq University of Porto ,
Department of Biological Sciences , Faculty of Pharmacy , Porto , Portugal.; bss
University of Porto, i3S-Instituto de Investigação e Inovação em Saúde , Porto ,
Portugal.; acd Medical University of Graz, Division of Cardiology , Graz ,
Austria.; alh Rice University, Chemical and Biomolecular Engineering , Houston , TX
, USA.; ali Rice University , Department of BioSciences , Houston , TX , USA.; bkw
University of Illinois at Chicago, Deprtment of Biochemistry and Molecular Genetics
, Chicago , IL , USA.; bql University of Oslo, Centre for Molecular Medicine Norway
(NCMM) , Oslo , Norway.; ayb Universitat Politècnica de València, COMAV Institute ,
Valencia , Spain.; bhi University of Chile, Faculty of Medicine, ICBM, Molecular
and Clinical Pharmacology , Santiago , Chile.; de Cedars-Sinai Medical Center ,
Department of Medicine , Los Angeles , CA , USA.; je Drexel University, College of
Medicine , Department of Pathology , Philadelphia , PA , USA.; rt INRA, UR1067,
Nutrion Métabolisme Aquaculture , St-Pée-sur-Nivelle , France.; caz Washington
University , Department of Medicine , St. Louis , MO , USA.; wg Johns Hopkins
University, School of Medicine, Institute for Cell Engineering and McKusick-Nathans
Institute of Genetic Medicine , Baltimore , MD , USA.; bmo University of Louisville
, Department of Physiology , Louisville , KY , USA.; bza University of Zurich,
Epidemiology, Biostatistics and Prevention Institute , Zurich , Switzerland.;
ec Centro de Investigaciones Biológicas (CSIC) , Department of Cellular and
Molecular Biology , Madrid , Spain.; wd Johns Hopkins University, School of
Medicine , Baltimore , MD , USA.; wu Kagoshima University, The Near-Future
Locomoter Organ Medicine Creation Course, Graduate School of Medical and Dental
Sciences , Kagoshima , Japan.; jn Dulbecco Telethon Institute and Telethon
Institute of Genetics and Medicine (TIGEM) , Naples , Italy.; bcb University of
Alabama at Birmingham , Department of Pathology , Birmingham , AL , USA.; zs
Lombardi Comprehensive Cancer Center, Georgetown University Medical Center ,
Department of Oncology , Washington, DC , USA.; att Thomas Jefferson University,
Sidney Kimmel Medical College , Philadelphia , PA , USA.; bnc University of
Maryland , Department of Nutrition and Food Science , College Park , MD , USA.; kd
Emory University, School of Medicine , Department of Pharmacology , Atlanta , GA ,
USA.; k Academia Sinica, Institute of Molecular Biology , Taipei , Taiwan.; qm
Hungkuang University , Department of Physical Therapy , Taichung , Taiwan.; agv
National University of Singapore , Department of Physiology , Yong Loo Lin School
of Medicine , Singapore.; agr National University of Ireland, Regenerative Medicine
Institute , Galway , Ireland.; aon Shanghai Jiao Tong University, School of
Medicine, Shanghai Institute of Hypertension , Shanghai , China.; apm Soochow
University, School of Pharmaceutical Science , Department of Pharmacology and
Laboratory of Aging and Nervous Diseases , Suzhou , China.; cr Capital Normal
University , Beijing , China.; ahu NINDS, National Institutes of Health, Synaptic
Function Section , Bethesda , MD , USA.; cbt Western University , Department of
Obstetrics and Gynaecology , London, ON , Canada.; aqi St. Paul's Hospital, Centre
for Heart Lung Innovation , Vancouver, BC , Canada.; beh University of British
Columbia , Department of Pathology and Laboratory Medicine , Vancouver, BC ,
Canada.; bzg US Food and Drug Administration, National Center for Toxicological
Research, Division of Systems Biology , Jefferson , AR , USA.; btx University of
Science and Technology of China, School of Life Sciences, and Hefei National
Laboratory for Physical Sciences at Microscale , Hefei, Anhui , China.; ajv
Pennsylvania State University, College of Medicine , Department of Cellular and
Molecular Physiology , Hershey , PA , USA.; ccf Yamaguchi University, Joint Faculty
of Veterinary Medicine, Laboratory of Veterinary Hygiene , Yamaguchi , Japan.; aea
NARO Institute of Floricultural Science , Tsukuba , Japan.; bpd University of
Nagasaki, Molecular and Cellular Biology, Graduate School of Human Health Science ,
Nagasaki , Japan.; ae National Chung-Hsing University, Institute of Biomedical
Sciences, College of Life Sciences , Taichung , Taiwan.; arj Taipei Medical
University , Department of Biochemistry , College of Medicine , Taipei City ,
Taiwan.; vt Jikei University School of Medicine, Research Center for Medical
Sciences, Division of Gene Therapy , Tokyo , Japan.; aun Tokyo Medical and Dental
University, Medical Research Institute, Pathological Cell Biology , Tokyo , Japan.;
an Amorepacific Corporation RandD Center, Bioscience Research Institute ,
Gyeonggi , Korea.; ji Duke University, Medical Center , Department of Immunology ,
Durham , NC , USA.; xw Kobe University, Graduate School of Health Sciences,
Laboratory of Pathology, Division of Medical Biophysics , Hyogo , Japan.; aud
Tohoku University, Laboratory of Bioindustrial Genomics, Graduate School of
Agricultural Science , Miyagi , Japan.; yx Kyoto University , Department of
Cardiovascular Medicine , Kyoto , Japan.; zb Kyushu University , Department of
Surgery and Science , Fukuoka , Japan.; aah Maastricht University, Medical Centre,
NUTRIM , Department of Molecular Genetics , Maastricht , The Netherlands.; cc
Boston University , Department of Medicine , Boston , MA , USA.; abj McGill
University , Department of Biochemistry , Montreal, Quebec , Canada.; xe Kaohsiung
Veterans General Hospital , Department of Medical Education and Research ,
Kaohsiung , Taiwan.; bkv University of Illinois at Chicago, Departments of
Ophthalmology and Microbiology and Immunology , Chicago , IL , USA.; aed National
Academy of Sciences of Ukraine , Department of Biotechnology and Microbiology ,
Lviv , Ukraine.; btm University of Rzeszow, Institute of Cell Biology , Rzeszow ,
Poland.; km Centre de Recherche des Cordeliers, Equipe 11 labellisée par la Ligue
Nationale contre le Cancer , Paris , France.; oc Gustave Roussy Cancer Campus ,
Villejuif , France.; sn INSERM, U1138 , Paris , France.; azv Université Paris
Descartes/Paris V , Paris , France.; bfo University of California San Diego ,
Department of Pathology , La Jolla , CA , USA.; ahk New York University School of
Medicine, Departments of Neuroscience and Physiology, and Psychiatry , New York ,
NY , USA.; id CSIR - Centre for Cellular and Molecular Biology , Hyderabad ,
India.; acf Medical University of Lodz , Department of Molecular Pathology and
Neuropathology , Lodz , Poland.; btt University of São Paulo, Ribeirão Preto
Medical School , Department of Physiology , Ribeirão Preto, São Paulo , Brazil.;
bxh University of Toulouse, INSERM UMR 1037, Cancer Research Center of Toulouse ,
Toulouse , France.; bsk University of Pittsburgh, School of Medicine , Department
of Pediatrics , Pittsburgh , PA , USA.; avg U.S. Food and Drug Administration,
Center for Biologics Evaluation and Research , Silver Spring , MD , USA.; avr Ulm
University, Institute of Pharmacology of Natural Compounds and Clinical
Pharmacology , Ulm , Germany.; adj MRC Human Immunology Unit, Weatherall Institute
of Molecular Medicine and BRC Translational Immunology Lab, NDM , Oxford , UK.; bdr
University of Bern, Institute of Pharmacology , Bern , Switzerland.; bde University
of Bari 'Aldo Moro', Division of Medical Genetics, DIMO, School of Medicine ,
Bari , Italy.; ajm Paris Descartes University-Sorbonne Paris Cité, Imagine
Institute , Paris , France.; bqr University of Oslo, Institute of Basic Medical
Sciences , Oslo , Norway.; x Albert Einstein College of Medicine , Bronx , NY ,
USA.; brv University of Pittsburgh Cancer Institute, Hillman Cancer Center Research
Pavilion , Pittsburgh , PA , USA.; akl Post Graduate Institute of Medical Education
and Research (PGIMER) , Department of Urology , Chandigarh , India.; wi Johns
Hopkins University, School of Medicine, Wilmer Eye Institute , Baltimore , MD ,
USA.; ahw North Dakota State University , Department of Chemistry and
Biochemistry , Fargo , ND , USA.; abg Mayo Clinic , Rochester , MN , USA.; akg
Polish Academy of Sciences, Institute of Biochemistry and Biophysics , Warsaw ,
Poland.; dr Centre for Cellular and Molecular Biology, Council of cientific and
Industrial Research , Hyderabad , India.; aqr Stellenbosch University , Department
of Physiological Sciences , Stellenbosch , South Africa.; apr Sorbonne Universités,
University Pierre and Marie Curie, Paris 6, Brain and Spine Institute, INSERM
U1127, CNRS UMR722 , Paris , France.; px Hong Kong Polytechnic University ,
Department of Health Technology and Informatics , Faculty of Health and Social
Sciences , Kowloon, Hong Kong.; ix Democritus University of Thrace, Medical
School , Department of Pathology , Alexandroupolis , Greece.; mf Gdansk University
of Technology , Department of Pharmaceutical Technology and Biochemistry , Gdansk ,
Poland.; bqv University of Ottawa , Department of Cellular and Molecular Medicine ,
Faculty of Medicine , Ottawa, Ontario , Canada.; aar Masaryk University ,
Department of Biology , Faculty of Medicine , Brno , Czech Republic.; ahy
Northeastern University , Department of Bioengineering , Boston , MA , USA.; lb
Federal University of São Paulo , Department of Pharmacology , Paulista School of
Medicine , São Paulo , Brazil.; acv Moffitt Cancer Center , Department of Tumor
Biology , Tampa , FL , USA.; aao Mahidol University, Salaya Campus, Institute of
Molecular Biosciences , Nakorn Pathom , Thailand.; ym KU Leuven , Department of
Imaging and Pathology , Leuven , Belgium.; bnw University of Michigan Medical
School , Department of Internal Medicine , Ann Arbor , MI , USA.; aif Norwegian
Veterinary Institute , Oslo , Norway.; rf Indian Institute of Science, Microbiology
and Cell Biology , Bangalore , India.; ky Ewha W. University, Brain and Cognitive
Sciences/Pharmacy , Seoul , Korea.; qx IIT University, School of Biotechnology ,
Orissa , India.; ap Applied Genetic Technologies Corporation , Alachua , FL , USA.;
anz Shandong University , Department of Toxicology , Jinan, Shandong , China.; yh
Korea University, Division of Life Sciences , Seoul , Korea.; pw Hong Kong Baptist
University, School of Chinese Medicine , Kowloon Tong, Hong Kong.; vm Jewish
General Hospital, Bloomfield Centre for Research in Aging, Lady Davis Institute for
Medical Research , Montreal, Quebec , Canada.; zd La Trobe University , Department
of Biochemistry and Genetics , La Trobe Institute for Molecular Science , Melbourne
, Victoria , Australia.; abv MD Anderson Cancer Center , Department of Cancer
Biology , Houston , TX , USA.; abx MD Anderson Cancer Center , Department of
Gynecologic Oncology and Reproductive Medicine , Houston , TX , USA.; agu National
University of Singapore , Department of Physiology , Singapore.; bfz University of
California San Diego, School of Medicine , Department of Psychiatry , La Jolla , CA
, USA.; anb Sapienza University of Rome , Department of Experimental Medicine ,
Rome , Italy.; bmx University of Manchester, Institute of Cancer Sciences, Faculty
of Medical and Human Sciences , Manchester , UK.; car Wake Forest University ,
Department of Surgery and Cancer Biology , Winston-Salem , NC , USA.; aan
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani,
Thailand.; bop
University of Minho, Molecular and Environmental Biology Centre (CBMA)/Department
of Biology , Braga , Portugal.; zl Leiden University, Institute of Biology , Leiden
, The Netherlands.; ald Radboud University Nijmegen Medical Center , Department of
Radiation Oncology , Nijmegen , The Netherlands.; bdg University of Basel ,
Biozentrum, Basel , Switzerland.; bta University of Rochester Medical Center ,
Department of Pathology and Laboratory Medicine , Rochester , NY , USA.; lh
Flinders University, School of Biological Sciences, Bedford Park , South
Australia , Australia.; bfp University of California San Diego , Department of
Pediatrics , Division of Infectious Diseases , La Jolla , CA , USA.; eo Charité -
Universitätsmedizin Berlin , Department of Anesthesiology and Intensive Care
Medicine , Campus Charité Mitte and Campus Virchow-Klinikum , Berlin , Germany.;
abe Mayo Clinic , Department of Neuroscience , Jacksonville , FL , USA.; blp
University of Kentucky , Department of Toxicology and Cancer Biology, Lexington ,
KY , USA.; ce Brescia University , Department of Clinical and Experimental Sciences
, Brescia , Italy.; bmf University of Lille, INSERM UMR1011, Institut Pasteur de
Lille, EGID , Lille , France.; bsm University of Pittsburgh, School of Medicine ,
Department of Surgery , Division of Endocrine Surgery , Pittsburgh , PA , USA.; gs
Cincinnati Children's Hospital Medical Center, Division of Experimental Hematology
and Cancer Biology , Cincinnati , OH , USA.; bwp University of Texas, Southwestern
Medical Center , Department of Internal Medicine , Dallas , TX.; bdi University of
Bayreuth , Department of Biochemistry , Bayreuth , Germany.; bfl University of
California San Diego , Department of Cellular and Molecular Medicine , La Jolla ,
CA , USA.; bv Biomedical Research Foundation of the Academy of Athens, Laboratory
of Neurodegenerative Diseases , Athens , Attiki , Greece.; bfg University of
California Irvine , Department of Psychiatry and Human Behavior , Irvine , CA ,
USA.; avy University of Texas, MD Anderson Cancer Center , Department of
Experimental Therapeutics , Houston , TX , USA.; ajd Oslo University Hospital ,
Department of Biochemistry , Institute for Cancer Research , Oslo , Norway.; tm
Institute of Nuclear Chemistry and Technology, Centre for Radiobiology and
Biological Dosimetry , Dorodna , Poland.; zm Leidos Biomedical Research, Inc.,
Frederick National Laboratory for Cancer Research, Nanotechnology Characterization
Lab, Cancer Research Technology Program , Frederick , MD , USA.; jv Edinburgh
Napier University, School of Life, Sport and Social Sciences , Edinburgh , UK.; hl
Columbia University , Department of Biological Sciences , New York , NY , USA.; hm
Columbia University , Department of Chemistry , New York , NY , USA.; vh J. Stefan
Institute , Department of Biochemistry and Molecular and Structural Biology ,
Ljubljana , Slovenia.; aaz Max Planck Institute of Biochemistry, Group Maintenance
of Genome Stability , Martinsried , Germany.; pn Heinrich-Heine-University,
Institute of Molecular Medicine , Düsseldorf , Germany.; bkj University of Helsinki
, Department of Biosciences , Helsinki , Finland.; aee National and Kapodistrian
University of Athens , Department of Cell Biology and Biophysics , Faculty of
Biology , Athens , Greece.; bcv University of Athens , Department of Cell Biology
and Biophysics , Faculty of Biology , Athens , Greece.; bau University Hospital
Aachen , IZKF and Department of Internal Medicine III , Aachen , Germany.; ain Ohio
State University, The James Comprehensive Cancer Center . Department of Molecular
Virology , Immunology and Medical Genetics and Department of Surgery , Division of
Surgical Oncology , Columbus , OH , USA.; aqt Stockholm University , Department of
Neurochemistry , Stockholm , Sweden.; br Binghamton University, State University of
New York , Binghamton , NY , USA.; tl Institute of Molecular Pathology and Biology,
FMHS UO , Hradec Kralove , Czech Republic.; bkm University of Hong Kong, Division
of Oral and Maxillofacial Surgery, Faculty of Dentistry , Hong Kong.; vq Jiangsu
University , Department of Immunology , Zhenjiang, Jiangsu , China.; cy Case
Western Reserve University, School of Medicine, Division of Infectious Diseases and
HIV Medicine , Department of Medicine , Cleveland , OH , USA.; atd The Scripps
Research Institute , Department of Neuroscience , Jupiter , FL , USA.; bvh
University of Sydney , Department of Neurogenetics , Kolling Institute , St
Leonards, NSW , Australia.; om Hallym University, School of Medicine , Department
of Physiology , Chuncheon , Korea.; cdb Zhejiang University, Sir Run Run Shaw
Hospital , Department of Medical Oncology , Hangzhou, Zhejiang , China.; acn
Medical University of Vienna , Department of Dermatology , Vienna , Austria.; hh
Columbia University Medical Center , Department of Neurology , New York , NY ,
USA.; aut Tongji University, School of Life Science and Technology , Shanghai ,
China.; bwh University of Texas, Medical Branch , Department of Microbiology and
Immunology , Galveston , TX , USA ; bkx University of Illinois at Chicago, Division
of Gastroenterology and Hepatology , Department of Medicine , Chicago , IL , USA.;
kg Emory University, School of Medicine, Winship Cancer Institute , Atlanta , GA ,
USA.; adx Nanjing University, School of Life Sciences, State Key Laboratory of
Pharmaceutical Biotechnology , Nanjing, Jiangsu , China.; bof University of
Michigan , Department of Radiation Oncology , Division of Radiation and Cancer
Biology , Ann Arbor , MI , USA.; aov Shanghai Veterinary Research Institute ,
Shanghai , China.; aak Macquarie University , Department of Biomedical Sciences ,
Faculty of Medicine and Health Sciences , Sydney , NSW , Australia.; ga Chinese
University of Hong Kong, Institute of Digestive Diseases , Shatin, Hong Kong.; xq
Keio University, School of Medicine , Medical Education Center , Tokyo , Japan.;
bwu University of Tokyo, Bioimaging Center, Graduate School of Frontier Sciences ,
Chiba , Japan.; atz Tohoku University , Department of Neurology , Sendai , Japan.;
alk RIKEN Global Research Cluster, Glycometabolome Team, Systems Glycobiology
Research Group , Saitama , Japan.; mx Georgetown University , Department of
Pharmacology and Physiology , Washington, DC , USA.; boa University of Michigan ,
Department of Microbiology and Immunology , Ann Arbor , MI , USA.; zv University
College London Cancer Institute , London , UK.; aac Lund University, Biomedical
Centre , Department of Experimental Medical Science , Lund , Sweden.; dr Centre for
Cellular and Molecular Biology, Council of Scientific and Industrial Research ,
Hyderabad , India.; byx University of York , Department of Biology , Heslington,
York , UK.; bmk University of Louisiana at Monroe, School of Pharmacy , Monroe , LA
, USA.; kj Eötvös Loránd University , Department of Anatomy , Cell and
Developmental Biology , Budapest , Hungary.; agp National University of Ireland,
Apoptosis Research Centre , Galway , Ireland.; akv Queen Mary University of London,
Barts Cancer Institute, Center for Molecular Oncology , London , UK.; bwl
University of Texas, Medical School at Houston, Division of Cardiovascular Medicine
, Department of Medicine , Houston , TX , USA.; anb Sapienza University of Rome ,
Department of Experimental Medicine , Rome , Italy.; azl Université Grenoble-Alpes,
CEA-DSV-iRTSV-BGE-GenandChem, INSERM, U1038 , Grenoble , France.; bjy University of
Glasgow, Cancer Research UK Beatson Institute , Glasgow , UK.; kk Eötvös Loránd
University , Department of Biological Anthropology , Budapest , Hungary.; aka
Pennsylvania State University, College of Medicine , Hershey Cancer Institute and
Department of Pediatrics , Hershey , PA , USA.; kj Eötvös Loránd University ,
Department of Anatomy , Cell and Developmental Biology , Budapest , Hungary.; aq
Asahi University , Department of Internal Medicine , Gifu , Japan.; xl Kawasaki
Medical School , Department of General Internal Medicine 4 , Okayama , Japan.; bje
University of Exeter, School of Biosciences , Exeter , UK.; bxr University of
Turin, Neuroscience Institute Cavalieri Ottolenghi , Turin , Italy.; azs Université
Paris Descartes, Institut Cochin, Faculté de Médecine Sorbonne Paris Cité , Paris ,
France.; arb Sun Yat-Sen University, School of Chemistry and Chemical Engineering ,
Guangzhou , China.; aks Qingdao University , Department of Neurology , Qingdao
Municipal Hospital, School of Medicine , Qingdao, Shandong Province , China.; aap
Malaysian Institute of Pharmaceuticals and Nutraceuticals , Pulau Pinang ,
Malaysia.; bag Universiti Sains Malaysia, Advanced Medical and Dental Institute,
Ministry of Science, Technology and Innovation , Pulau Pinang , Malaysia.; buf
University of South Alabama, Mitchell Cancer Institute , Mobile , AL , USA.; a
A*STAR (Agency for Science, Technology and Research), Institute of Molecular and
Cell Biology , Singapore.; agx National University of Singapore, Department of
Microbiology and Immunology, Yong Loo Lin School of Medicine, National University
Health System (NUHS) , Singapore.; aup Tokyo Metropolitan Institute of Medical
Scien e, Laboratory of Protein Metabolism , Tokyo , Japan.; yr Kyoto Prefectural
University of Medicine , Department of Basic Geriatrics , Kyoto , Japan.; bsb
University of Pittsburgh , Department of Surgery , Hillman Cancer Center ,
Pittsburgh , PA , USA.; fw Chinese Academy of Sciences, State Key Laboratory of
Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental
Biology , Beijing , China.; hh Columbia University Medical Center , Department of
Neurology , New York , NY , USA.; wq Juntendo University, School of Medicine ,
Department of Cell Biology and Neuroscience , Tokyo , Japan.; ps Hirosaki
University Graduate School of Medicine , Hirosaki , Japan.; aav Massachusetts
General Hospital and Harvard
Medical School, Experimental Therapeutics and Molecular Imaging Laboratory,
Neuroscience Center , Charlestown , MA , USA.; bjf University of Extremadura ,
Department of Medicine , Faculty of Veterinary Medicine , Cáceres , Spain.; z
Albert Einstein College of Medicine , Department of Developmental and Molecular
Biology , Institute for Aging Studies , Bronx , NY , USA.; cl Brown University ,
Department of Ecology and Evolutionary Biology , Providence , RI , USA.; ade Icahn
School of Medicine at Mount Sinai , Department of Pharmacology and Systems
Therapeutics , New York , NY , USA.; lp Foundation for Research and Technology -
Hellas , Heraklion, Crete , Greece.; bim University of Crete , Department of Basic
Sciences , Faculty of Medicine , Heraklion, Crete , Greece.; bin University of
Crete, Institute of Molecular Biology and Biotechnology , Heraklion, Crete ,
Greece.; ave Tufts University, USDA Human Nutrition Research Center on Aging ,
Boston , MA , USA.; bdt University of Birmingham, Institute of Immunology and
Immunotherapy , Birmingham, West Midlands , UK.; ji Duke University, Medical Center
, Department of Immunology , Durham , NC , USA.; jj Duke University, Medical Center
, Department of Medicine , Durham , NC , USA.; jk Duke University, Medical Center ,
Department of Molecular Genetics and Microbiology , Durham , NC , USA.; jo Durham
VA Medical Center, GRECC , Durham , NC , USA.; aqa Howard Hughes Medical Institute,
St. Jude Children's Research Hospital, Cell and Molecular Biology , Memphis , TN ,
USA.; zr Liverpool School of Tropical Medicine , Department of Parasitology ,
Liverpool, Merseyside , UK.; aec Nasonova Research Institute of Rheumatology,
Immunology and Molecular Biology Laboratory , Moscow , Russia.; cs Cardiff
University, Heath Park, Institute of Cancer and Genetics , Cardiff , Wales , UK.;
sf INSERM U955, Faculté de Médecine de Créteil, UMR-S955 , Créteil , France.; baa
Université Paris-Est, Institut Mondor de Recherche Biomédicale , Paris , France.;
bmm University of Louisville , Department of Medicine (Hem-Onc) , Louisville , KY ,
USA.; gh Chulalongkorn University , Department of Clinical Chemistry , Faculty of
Allied Health Sciences , Bangkok , Thailand.; bvs University of Texas, Health
Science Center at Houston, Center for Human Genetics, Institute of Molecular
Medicine , Houston , TX , USA.; aca Medical College of Wisconsin , Department of
Pediatrics , Milwaukee , WI , USA.; bmg University of Limoges , Department of
Histology and Cell Biology , Limoges , France.; blb University of Insubria ,
Department of Biotechnology and Life Sciences , Varese , Italy.; bd Baylor
University Medical Center , Department of Internal Medicine , Division of
Gastroenterology, Baylor Research Institute , Dallas , TX.; bst University of
Pretoria , Department of Physiology , Pretoria, Gauteng , South Africa.; hg
Colorado Mesa University , Department of Biological Sciences , Grand Junction ,
CO , USA.; axc Universidad Federal do Rio Grande do Sul (UFRGS) , Department of
Biophysics and Center of Biotechnology , Porto Alegre , Brazil.; bph University of
Nebraska Medical Center , Department of Internal Medicine , Omaha , NE , USA.; bih
University of Colorado, School of Medicine , Department of Pharmacology , Aurora ,
CO , USA.; bew University of California Berkeley , Department of Molecular and Cell
iology , Berkeley , CA , USA.; or Hannover Medical School, Institute of Molecular
and Translational Therapeutic Strategies (IMTTS) , Hannover , Germany.; mr Georg-
August-University Göttingen, Institute of Cellular Biochemistry , Göttingen ,
Germany.; qz Imperial College London, MRC Centre for Molecular Bacteriology and
Infection , London , UK.; fq Chinese Academy of Sciences, Key Laboratory of
Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology,
Shanghai Institutes for Biological Sciences , Shanghai , China.; bdy University of
Bonn, Institute of Reconstructive Neurobiology , Bonn , Germany.; bfx University of
California San Diego, San Diego Center for Systems Biology , La Jolla , CA , USA.;
bpy University of North Carolina , Department of Microbiology-Immunology , Chapel
Hill , NC , USA.; bqa University of North Carolina, Lineberger Comprehensive Cancer
Center, Institute of Inflammatory Diseases, Center for Translational Immunology ,
Chapel Hill , NC , USA.; ht Concordia University , Biology Department , Montreal,
Quebec , Canada.; bei University of British Columbia , Department of Psychiatry ,
Vancouver, BC , Canada.; cac Virginia Commonwealth University, Internal Medicine,
VCU Pauley Heart Center , Richmond , VA , USA.; zu London Research Institute,
Cancer Research UK , London , UK.; vo Jewish General Hospital , Department of
Oncology , Montreal, Quebec , Canada.; abr McGill University, Lady Davis Institute
for Medical Research , Montreal, Quebec , Canada.; ajb Oslo University Hospital,
Centre for Cancer Biomedicine , Oslo , Norway.; asy The Norwegian Radium Hospital,
Faculty of Medicine , Oslo , Norway.; bqm University of Oslo , Department of
Biochemistry , Institute for Cancer Research , Oslo , Norway.; if CSS-Mendel
Institute, Neurogenetics Unit , Rome , Italy.; rz INSERM U1138 , Paris , France.;
ana Sapienza University of Rome , Department of Clinical and Molecular Medicine ,
Rome , Italy.; bmw University of Manchester, Faculty of Life Sciences ,
Manchester , UK.; bny University of Michigan , Ann Arbor , MI , USA.; bdm
University of Belgrade, School of Medicine , Belgrade , Serbia.; la Federal
University of Rio de Janeiro, Institute of Biophysics Carlos Chagas Filho,
Laboratory of Immunoreceptors and Signaling , Rio de Janeiro , Brazil.; tb
Institute of Advanced Chemistry of Catalonia, Spanish Research Council (IQAC-
CSIC) , Department of Biomedicinal Chemistry , Barcelona , Spain.; asg Texas A&M
University Health Science Center, Center for Translational Cancer Research,
Institute of Bioscience and Technology , Houston , TX , USA.; alf Regina Elena
National Cancer Institute, Experimental Chemotherapy Laboratory , Rome , Italy.;
awj Universidad de Chile, Advanced Center for Chronic Diseases (ACCDiS), Facultad
de Ciencias Químicas y Farmacéuticas , Santiago , Chile.; awn Universidad de Chile,
Instituto de Nutrición y Tecnología de los Alimentos (INTA) , Santiago , Chile.;
bcv University of Athens , Department of Cell Biology and Biophysics , Faculty of
Biology , Athens , Greece.; bav University Hospital Center, University of Lausanne,
Clinic of Neonatology , Department of Pediatrics and Pediatric Surgery , Lausanne ,
Switzerland.; aen National Cheng Kung University, Institute of Clinical Medicine ,
Tainan , Taiwan.; bdo University of Bern, Division of Experimental Pathology,
Institute of Pathology , Bern , Switzerland.; ej Chang Gung University , Department
of Biochemistry , College of Medicine , Taoyuan , Taiwan.; adp Nagasaki University,
Division of Dental Pharmacology, Graduate School of Biomedical Sciences ,
Nagasaki , Japan.; brx University of Pittsburgh Medical Center , Department of
Surgery , Pittsburgh , PA , USA.; bwk University of Texas, Medical School at
Houston , Department of Neurobiology and Anatomy , Houston , TX , USA.; aos
Shanghai Jiao Tong University, School of Medicine, Renji Hospital , Shanghai ,
China.; agm National Tsing Hua University , Department of Chemical Engineering ,
Hsinchu , Taiwan.; bdd University of Bari 'Aldo Moro' , Department of Biomedical
Scienc s and Clinical Oncology , Bari , Italy.; buv University of Southampton,
Centre for Biological Sciences, Highfield Campus , Southampton , UK.; vh J. Stefan
Institute , Department of Biochemistry and Molecular and Structural Biology ,
Ljubljana , Slovenia.; vh J. Stefan Institute , Department of Biochemistry and
Molecular and Structural Biology , Ljubljana , Slovenia.; bem University of British
Columbia, Michael Smith Laboratories , Vancouver, British Columbia , Canada.; ajc
Oslo University Hospital, Centre for Immune Regulation , Oslo , Norway.; bmr
University of Louisville, School of Medicine , Department of Physiology and
Biophysics , Louisville , KY , USA.; pv Hokkaido University, Research Faculty of
Agriculture , Sapporo , Japan.; wq Juntendo University, School of Medicine ,
Department of Cell Biology and Neuroscience , Tokyo , Japan.; ajh Otto-von-
Guericke-University Magdeburg , Department of General , Visceral and Vascular
Surgery , Magdeburg , Germany.; wp Juntendo University, Graduate School of
Medicine, Laboratory of Proteomics and Biomolecular Science , Tokyo , Japan.; alm
Ritsumeikan University , Department of Biotechnology , Shiga , Japan.; aig Obihiro
University of Agriculture and Veterinary Medicine, National Research Center for
Protozoan Diseases , Obihiro, Hokkaido , Japan.; bh Beechcroft, Fulbourn Hospital ,
Cambridge , UK.; bqu University of Osnabrueck, Fachbereich Biologie/Chemie ,
Osnabrueck , Germany.; lb Federal University of São Paulo , Department of
Pharmacology , Paulista School of Medicine , São Paulo , Brazil.; yv Kyoto Sangyo
University , Department of Molecular Biosciences , Faculty of Life Sciences , Kyoto
, Japan.; bur University of South Florida , Department of Molecular Medicine ,
Tampa , FL , USA.; dm Central University of Venezuela, Institute for Anatomy ,
Caracas , Venezuela.; qw IFOM - The FIRC Institute of Molecular Oncology , Milan ,
Italy.; bep University of Buenos Aires, National Council for Scientific and
Technical Research (CONICET), Institute for Biochemistry and Molecular Medicine ,
Department of Pathophysiology , School of Pharmacy and Biochemistry , Buenos
Aires , Argentina.; ti Institute of Microbiology ASCR, v.v.i. , Prague , Czech
Republic.; xj Karolinska Institute , Department of Physiology and Pharmacology ,
Stockholm , Sweden.; bpc University of Murcia-IMIB Virgen de la Arrixaca Hospital,
Human Anatomy and Psycobiology Department, Cell Therapy and Hematopoietic
Transplantation Unit , Murcia , Spain.; btq University of Salerno, Section of
Neurosciences , Department of Medicine and Surgery , Salerno , Italy.; azf
Université de Nantes, CRCNA, UMRINSERM 892/CNRS 6299 , Nantes , France.; tq
Instituto de Investigaciones Biomédicas Alberto Sols, CSIC/UAM , Madrid , Spain.;
yj KU Leuven , Clinical Division and Laboratory of Intensive Care Medicine ,
Department Cellular and Molecular Medicine , Leuven , Belgium.; yi KU Leuven and
VIB, Vesalius Research Center, Laboratory of Neurobiology , Leuven , Belgium.; l
Academic Medical Center , Department of Gastroenterology and Hepatology , Amsterdam
, The Netherlands.; ju Ecole Polytechnique Federale de Lausanne, Global Health
Institute, School of Life Sciences , Lausanne , Switzerland.; bkf University of
Groningen, Molecular Cell Biology , Groningen , The Netherlands.; kn Erasmus MC-
University Medical Center Rotterdam , Department of Surgery , Rotterdam , The
Netherlands.; bfd University of California Davis, Mann Laboratory , Department of
Plant Sciences , Davis , CA , USA.; cal VU University Medical Center , Department
of Molecular Cell Biology and Immunology , Amsterdam , The Netherlands.; asv The
Helen F. Graham Cancer Center , Newark , DE , USA.; bis University of Delaware ,
Department of Biological Sciences , Newark , DE , USA.; bit University of Delaware,
The Center for Translational Cancer Research , Newark , DE , USA.; bzt Vanderbilt
University, School of Medicine , Department of Pathology , Microbiology and
Immunology , Nashville , TN , USA.; mk Genentech Inc. , Department of Immunology ,
South San Francisco , CA , USA.; n Ghent University , Department of Biomedical
Molecular Biology , Inflammation Research Center, VIB, Methusalem Program , Gent ,
Belgium.; bbi University Hospitals Leuven , Department of Neurology , Leuven ,
Belgium.; bmc University of Leuven , Department of Neurosciences , Leuven ,
Belgium.; yj KU Leuven , Clinical Division and Laboratory of Intensive Care
Medicine , Department Cellular and Molecular Medicine , Leuven , Belgium.; ic CSIC-
UAM and CIBERER, Institute for Biomedical Research "Alberto Sols" , Madrid ,
Spain.; bsp University of Porto, Cancer Drug Resistance Group, IPATIMUP - Institute
of Molecular Pathology and Immunology , Porto , Portugal.; bsq University of
Porto , Department of Biological Sciences , Faculty of Pharmacy , Porto ,
Portugal.; bss University of Porto, i3S-Instituto de Investigação e Inovação em
Saúde , Porto , Portugal.; mq Georg-August-University Göttingen , Department of
Nephrology and Rheumatology , Göttingen , Germany.; pe Harvard Medical School,
Ophthalmology , Boston , MA , USA.; bhq University of Coimbra, CNC-Center for
Neuroscience and Cell Biology , Cantanhede , Portugal.; hs Complutense University,
Instituto de Investigaciones Sanitarias San Carlos (IdISSC) , Department of
Biochemistry and Molecular Biology I , School of Biology , Madrid , Spain.; bcv
University of Athens , Department of Cell Biology and Biophysics , Faculty of
Biology , Athens , Greece.; bnm University of Massachusetts, Medical School ,
Department of Molecular , Cell and Cancer Biology , Worcester , MA , USA.; kl
Eötvös Loránd University , Department of Genetics , Budapest , Hungary.; bbm
University Medical Center Groningen, University of Groningen , Department of
Hematology , Groningen , The Netherlands.; c Aarhus University Hospital, Department
of Nuclear Medicine and PET Center , Aarhus , Denmark.; bvz University of Texas,
Health Sciences Center-Houston (UTHSC) , Department of Integrative Biology and
Pharmacology , Houston , TX , USA.; pl Heinrich Heine University, Institute of
Clinical Chemistry and Laboratory Diagnostic, Medical Faculty , Duesseldorf ,
Germany.; vg IUF-Leibniz Research Institute for Environmental Medicine ,
Duesseldorf , Germany.; axy Universitat Autònoma de Barcelona, Institut de
Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular ,
Bellaterra (Barcelona) , Spain.; nu Gonçalo Moniz Research Center, Oswaldo Cruz
Foundation, FIOCRUZ-BA, Laboratory of Pathology and Biointervention , Salvador,
BA , Brazil.; ayt Université de Limoges, EA 3842, LHCP, Faculté de Médecine ,
Limoges , France.; cn Budapest University of Technology and Economics, Institute of
Enzymology, RCNC , HAS and Department of Applied Biotechnology , Budapest ,
Hungary.; abw MD Anderson Cancer Center , Department of Genomic Medicine ,
Houston , TX , USA.; bbv University Montpellier, UMR5235 , Montpellier , France.;
axo Universidade Nova de Lisboa, CEDOC, NOVA Medical School , Lisboa , Portugal.;
byq Washington University in St Louis, Department of Biology, St. Louis, MO , USA.;
bvu University of Texas, Health Science Center at Houston, School of Dentistry ,
Houston , TX , USA.; dk Central Michigan University, College of Medicine , Mt.
Pleasant , MI , USA.; ax Autonomous University of Barcelona (UAB) , Department of
Biochemistry and Molecular Biology , Barcelona , Spain.; cz Catalan Institution for
Research and Advanced Studies (ICREA) , Barcelona , Spain.; ahc Neurodegenerative
Diseases Research Group, Vall d'Hebron Research Institute-CIBERNED , Barcelona ,
Spain.; ami SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-
UCLM-JCCM , Ciudad Real , Spain.; se INSERM U916, Université de Bordeaux, Institut
Européen de Chimie et Biologie , Pessac , France.; z Albert Einstein College of
Medicine , Department of Developmental and Molecular Biology , Institute for Aging
Studies , Bronx , NY , USA.; bxi University of Toulouse, INSERM UMR 1048 , Toulouse
, France.; brd University of Padova , Department of Woman's and Child's Health ,
Laboratory of Oncohematology , Padova , Italy.; uk IRCCS Santa Lucia Foundation ,
Rome , Italy.; bon University of Milan, Istituto Auxologico Italiano , Department
of Clinical Sciences and Community Health , Milan , Italy.; brl University of
Pennsylvania, Abramson Cancer Center , Philadelphia , PA , USA.; xy Komarov
Botanical Institute RAS, Plant Ecological Physiology Laboratory , Saint
Petersburg , Russian Federation.; eo Charité - Universitätsmedizin Berlin ,
Department of Anesthesiology and Intensive Care Medicine , Campus Charité Mitte and
Campus Virchow-Klinikum , Berlin , Germany.; aab Ludwig-Maximilians-University
Munich , Department of Pharmacy , Munich , Germany.; bcs University of Arkansas for
Medical Sciences , Department of Microbiology and Immunology , Little Rock , AR ,
USA.; bpv University of Nice-Sophia Antipolis, IRCAN , Nice , France.; amt Sanford
Burnham Prebys Medical Discovery Institute , La Jolla , CA , USA.; aaw
Massachusetts General Hospital, Division of Infectious Disease , Boston , MA ,
USA.; bao University College Cork, School of Pharmacy , Department of Pharmacology
and Therapeutics , Cork , Ireland.; asf Texas A&M Health Science Center, Institute
of Biosciences and Technology , Houston , TX , USA.; bsv University of Queensland,
Australian Infectious Diseases Research Centre and School of Chemistry and
Molecular Biosciences , Brisbane , Queensland , Australia.; bdw University of
Bonn , Department of Neurology , Bonn , Germany.; au Asia University , Department
of Biotechnology , Taichung , Taiwan.; fc China Medical University, School of
Chinese Medicine , Taichung , Taiwan.; api Sixth Affiliated Hospital of Sun Yat-Sen
University, Gastrointestinal Institute , Department of Radiation Oncology ,
Guangzhou, Guangdong , China.; akr Qilu Hospital of Shandong University ,
Department of Traditional Chinese Medicine , Jinan , China.; cbe Washington
University, School of Medicine, Departments of Obstetrics and Gynecology, and
Pathology and Immunology , St. Louis , MO , USA.; eg Chang Gung University, Chang
Gung Memorial Hospital , Department of Cardiology , Internal Medicine , Taoyuan ,
Taiwan.; fo Chinese Academy of Sciences, Institute of Plant Physiology and Ecology,
Shanghai Institutes for Biological Sciences , Shanghai , China.; bhl University of
Cincinnati College of Medicine , Department of Cancer Biology , Cincinnati , OH ,
USA.; jq East China Normal University, School of Life Science , Shanghai , China.;
ot Harbin Medical University, College of Bioinformatics Science and Technology ,
Harbin, Heilongjiang , China.; ase Texas A&M Health Science Center, Center for
Cancer and Stem Cell Biology, Institute of Biosciences and Technology , Houston ,
TX , USA.; fn Chinese Academy of Sciences, Institute of Microbiology , Beijing ,
China.; apj Soochow University, College of Pharmaceutical Sciences , Jiangsu ,
China.; aot Shanghai Medical School of Fudan University , Department of Anatomy ,
Histology and Embryology , Shanghai , China.; ahx North Shore University Hospital ,
Department of Emergency Medicine , Manhasset , NY , USA.; ajw Pennsylvania State
University, College of Medicine , Department of Pediatrics , Hershey , PA , USA.;
bun University of South Dakota, Sanford School of Medicine, Division of Basic
Biomedical Sciences , Vermillion , SD , USA.; agn National Tsing Hua University,
Institute of Biotechnology, Institute of Systems Neuroscience , and Department of
Life Science , HsinChu City , Taiwan.; bbu University Montpellier 1, INSERM U1051 ,
Montpellier , France.; ey China Agricultural University, College of Animal Science
and Technology, State Key Laboratory of Animal Nutrition , Beijing , China.; jm
Duke-NUS Graduate Medical School, Cancer and Stem Cell Biology Program ,
Singapore.; lr Fourth Military Medical University , Department of Oral Anatomy and
Physiology and TMD , College of Stomatology , Xi'an , China.; agj National Taiwan
University, Graduate Institute of Brain and Mind Sciences, College of Medicine ,
Taipei , Taiwan.; ma Fudan University, Cancer Center , Department of Integrative
Oncology , Shanghai , China.; bwm University of exas, Southwestern Medical Center
at Dallas , Department of Dermatology , Dallas , TX.; fi Chinese
Academy of Sciences, CAS Key Laboratory of Infection and Immunity, Institute of
Biophysics , Beijing , China.; k Academia Sinica, Institute of Molecular Biology ,
Taipei , Taiwan.; aph Sir Runrun Shaw Hospital, Medical School of Zhejiang
University , Department of Medical Oncology , Hangzhou , China.; ccp Zhejiang
Cancer Hospital , Department of Medical Oncology , Hangzhou , China.; ccw Zhejiang
University, Institute of Insect Science , Hangzhou , China.; ch Brigham and Women's
Hospital, Harvard Medical School , Department of Neurosurgery , Boston MA.; buo
University of South Dakota , Vermillion , SD , USA.; avb Tsinghua University,
School of Life Sciences , Beijing , China.; akb Pennsylvania State University ,
Department of Biochemistry and Molecular Biology , Center for Eukaryotic Gene
Regulation , University Park , PA , USA.; bk Beijing Jishuitan Hospital ,
Department of Molecular Orthopedics , Beijing Institute of Traumatology and
Orthopedics , Beijing , China.; aeo National Cheng Kung University, Medical College
, Department of Environmental and Occupational Health , Tainan , Taiwan.; jp DZNE,
German Center for Neurodegenerative Diseases, and CAESAR Research Center , Bonn ,
Germany.; bkl University of Hong Kong , Department of Pharmacology and Pharmacy ,
Hong Kong , China.; bef University of British Columbia , Department of Medicine and
Brain Research Center , Vancouver, BC , Canada.; bxd University of Toronto,
Hospital for Sick Children , Toronto, Ontario , Canada.; en Changzheng Hospital,
The Second Military Medical University , Department of Cardiothoracic Surgery ,
Shanghai , China.; tw Instituto Leloir , Buenos Aires , Argentina.; bds University
of Birmingham, Institute of Biomedical Research, Institute of Cancer and Genomic
Sciences, College of Medical and Dental Sciences , Edgbaston, Birmingham , UK.; byb
University of Utah, School of Medicine , Department of Pathology , Salt Lake City ,
UT , USA.; akx Queen Mary University of London, Blizard Institute, Flow Cytometry
Core Facility , London , UK.; wn Juntendo University, Graduate School of Medicine ,
Department of Metabolism and Endocrinology , Tokyo , Japan.; yt Kyoto Prefectural
University of Medicine , Department of Basic Geriatrics, Kyoto , Japan.; aul Tokyo
Medical and Dental University, Center for Brain Integration Research, Bunkyo ,
Tokyo , Japan.; bhk University of Cincinnati College of Medicine, Cincinnati
Children's Research Foundation and Department of Pediatrics , Cincinnati , OH ,
USA.; bhz University of Colorado Denver, Division of Medical Oncology , Department
of Medicine , Aurora , CO , USA.; adw Nanjing University, Jiangsu Key Laboratory of
Molecular Medicine, Medical School and the State Key Laboratory of Pharmaceutical
Biotechnology , Nanjing, Jiangsu Province , China.; bbe University Hospital of
Muenster , Department of Internal Medicine D , Molecular Nephrology , Muenster ,
Germany.; cbc Washington University, School of Medicine , Department of Neurology ,
St. Louis , MO , USA.; lv Freie Universität Berlin, Institute of Pharmacy
(Pharmacology and Toxicology) , Berlin , Germany.; axf Universidade de Brasília ,
Departamento de Biologia Celular , Brasília, DF , Brazil.; btv University of
Science and Technology of China , Anhui , China.; boc University of Michigan ,
Department of Molecular , Cellular, and Developmental Biology , Ann Arbor , MI ,
USA.; bog University of Michigan, Life Sciences Institute , Ann Arbor , MI , USA.;
abv MD Anderson Cancer Center , Department of Cancer Biology , Houston , TX , USA.;
abx MD Anderson Cancer Center , Department of Gynecologic Oncology and Reproductive
Medicine , Houston , TX , USA.; bdh University of Bayreuth, Cell Biology , Bayreuth
, Germany.; aqn Stanford University, School of Medicine , Stanford , CA , USA.; bnr
University of Melbourne , Department of Pathology , Parkville , Victoria ,
Australia.; ame Rutgers University, The State University of New Jersey, Rutgers
Cancer Institute of New Jersey , N w Brunswick , NJ , USA.; atb The Scripps
Research Institute , Department of Immunology and Microbial Science , La Jolla , CA
, USA.; adl MRC Mitochondrial Biology Unit , Cambridge , UK.; baf Université Paris-
Sud, Université Paris-Saclay, UMR 8126CNRS, Institut Gustave Roussy , Villejuif ,
France.; aaq Mannheim University of Applied Sciences, Institute of Molecular and
Cell Biology , Mannheim , Germany.; l Academic Medical Center , Department of
Gastroenterology and Hepatology , Amsterdam , The Netherlands.; biw University of
East Anglia, Norwich Medical School , Norfolk , UK.; amt Sanford Burnham Prebys
Medical Discovery Institute , La Jolla , CA , USA.; bjb University of Edinburgh,
Edinburgh Cancer Research UK Centre, MRC Institute of Genetics and Molecular
Medicine , Edinburgh , UK.; ln Forschungszentrum Juelich, ICS-6/Structural
Biochemistry , Juelich , Germany.; pm Heinrich-Heine-University , Institut für
Physikalische Biologie, Duesseldorf , Germany.; bw Biomolecular Sciences and
Biotechnology Institute (GBB) , Groningen , The Netherlands.; bkf University of
Groningen, Molecular Cell Biology , Groningen , The Netherlands.; bzh US Food and
Drug Administration, National Center for Toxicology Research, Division of
Microbiology , Jefferson , AR , USA.; afm National Institutes of Health, Laboratory
of Clinical Infectious Diseases, National Institute of Allergy and Infectious
Diseases , Bethesda , MD , USA.; alt Ruhr University Bochum , Department of
Molecular Cell Biology , Institute of Biochemistry and Pathobiochemistry , Bochum ,
Germany.; cbl Weill Cornell Medical College , Department of Obstetrics and
Gynecology , New York , NY , USA.; bjl University of Florida , Department of Animal
Sciences , IFAS/College of Agriculture and Life Science , Gainesville , FL , USA.;
aba Max Planck Institute of Biochemistry, Molecular Membrane and Organelle
Biology , Martinsried , Germany.; bsw University of Queensland, Australian
Institute for Bioengineering and Nanotechnology (AIBN) , Brisbane , Australia.; adz
Nanyang Technological University, School of Biological Sciences , Singapore.; we
Johns Hopkins University, School of Medicine , Department of Physiology and Center
for Metabolism and Obesity Research , Baltimore , MD , USA.; wz Kanazawa
University, Cell-bionomics Unit and Laboratory of Molecular and Cellular Biology ,
Department of Biology , Faculty of Natural Systems, Institute of Science and
Engineering , Ishikawa , Japan.; aai Macau University of Science and Technology,
State Key Laboratory of Quality Research in Chinese Medicine , Macau , China.; ba
Baker IDI Heart and Diabetes Institute, Molecular Cardiology Laboratory , Melbourne
, Australia.; zj Lancaster University, Faculty of Health and Medicine, Division of
Biomedical and Life Sciences , Lancaster , UK.; zz Louisiana State University
Health Sciences Center, Neuroscience Center of Excellence , New Orleans , LA ,
USA.; qt Icahn School of Medicine at Mount Sinai , New York , NY , USA.; cbj Wayne
State University, School of Medicine , Departments of Oncology and Pathology ,
Detroit , MI , USA.; lz Fudan University Shanghai Medical College, Key Laboratory
of Molecular Virology , Shanghai , China.; bnf University of Maryland, School of
Medicine , Department of Anesthesiology and Center for Shock , Trauma and
Anesthesiology Research (STAR), National Study Center for Trauma and EMS ,
Baltimore , MD , USA.; bty University of Science and Technology of China, School of
Life Sciences , Hefei, Anhui , China.; bqc University of North Dakota , Department
of Biomedical Sciences , School of Medicine and Health Sciences , Grand Forks ,
ND , USA.; cbs Wenzhou Medical University, School of Optometry and Ophthalmology
and Eye Hospital , Wenzhou, Zhejiang , China.; fx Chinese University of Hong Kong ,
Department of Anaesthesia and Intensive Care , Hong Kong.; asq The First Affiliated
Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery ,
Department of General Surgery , Harbin , China.; ez China Agricultural University ,
Department of Animal Nutrition and Feed cience , Beijing , China.; bsp University
of Porto, Cancer Drug Resistance Group, IPATIMUP - Institute of Molecular Pathology
and Immunology , Porto , Portugal.; bss University of Porto, i3S-Instituto de
Investigação e Inovação em Saúde , Porto , Portugal.; ow Harvard Medical School and
Broad Institute , Boston , MA , USA.; fn Chinese Academy of Sciences, Institute of
Microbiology , Beijing , China.; bfi University of California Los Angeles ,
Department of Medicine , Los Angeles , CA , USA.; aog Shanghai Jiao Tong
University, School of Biomedical Engineering and Med-X Research Institute ,
Shanghai , China.; aoq Shanghai Jiao Tong University, State Key Laboratory of
Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji
Hospital, School of Medicine , Shanghai , China.; atf The Third Affiliated Hospital
of Guangzhou Medical University , Department of Clinical Laboratory Medicine ,
Guangzhou, Guangdong , China.; aoz Sichuan University, Aging Research Group, State
Key Lab for Biotherapy, West China Hospital , Chengdu , China.; anh Second Military
Medical University , Department of Cardiothoracic Surgery , Changzheng Hospital ,
Shanghai , China.; ard Sun Yat-Sen University, State Key Laboratory of Biocontrol,
School of Life Sciences , Guangzhou , China.; fm Chinese Academy of Sciences,
Institute of Hydrobiology , Wuhan, Hubei , China.; boe University of Michigan ,
Department of Radiation Oncology , Ann Arbor , MI , USA.; aoh Shanghai Jiao Tong
University, School of Life Sciences and Biotechnology , Shanghai , China.; bqj
University of Oklahoma, Health Sciences Center, Section of Molecular Medicine ,
Department of Medicine , Oklahoma City , OK , USA.; bu Biomedical Research
Foundation of the Academy of Athens, Center of Clinical, Experimental Surgery and
Translational
Research , Athens , Greece.; bjt University of Fribourg , Department of Medicine ,
Division of Physiology , Fribourg , Switzerland.; gc Chinese University of Hong
Kong, School of Chinese Medicine, Faculty of Medicine , Shatin, NT, Hong Kong.; aoo
Shanghai Jiao Tong University, School of Medicine, Shanghai Institute of Immunology
, Shanghai , China.; md Fudan University , Department of Neurosugery , Shanghai ,
China.; boc University of Michigan , Department of Molecular , Cellular, and
Developmental Biology , Ann Arbor , MI , USA.; ou Harbin Medical University ,
Department of Immunology , Heilongjiang Provincial Key Laboratory for Infection and
Immunity , Harbin , China.; bqh University of Oklahoma Health Sciences Center ,
Department of Medicine , Oklahoma City , OK , USA.; aox Shantou University Medical
College , Department of Biochemistry and Molecular Biology , Shantou , China.; nf
Georgia Regents University, Medical College of Georgia , Department of Cellular
Biology and Anatomy , Augusta , GA , USA.; ng Georgia Regents University, Medical
College of Georgia , Department of Medicine , Augusta , GA , USA.; bli University
of Kansas and University of Kansas Cancer Center , Departments of Molecular
Biosciences and Radiation Oncology , Lawrence , KS , USA.; abg Mayo Clinic ,
Rochester , MN , USA.; pb Harvard Medical School , Department of Cell Biology ,
Boston , MA , USA.; vu Jilin Medical University, Medical Research Laboratory ,
Jilin City, Jilin Province , China.; bca University of Alabama at Birmingham ,
Department of Medicine , Division of Hematology and Oncology, Comprehensive Cancer
Center , Birmingham , AL , USA.; boc University of Michigan , Department of
Molecular , Cellular, and Developmental Biology , Ann Arbor , MI , USA.; bog
University of Michigan, Life Sciences Institute , Ann Arbor , MI , USA.; qk
Huazhong University of Science and Technology , Department of Biomedical
Engineering , College of Life Science and Technology , Wuhan, Hubei , China.; pt
Hokkaido University Graduate School of Medicine , Department of Obstetrics and
Gynecology , Sapporo , Hokkaido , Japan.; ho Columbia University , Department of
Neurology , New York , NY , USA.; adr Nagoya University, Research Institute of
Environmental Medicine, Nagoya , Aichi , Japan.; wr Juntendo University, School of
Medicine , Department of Gastroenterology , Tokyo , Japan.; amb Rutgers University,
Molecular Biology and Biochemistry , Piscataway , NJ , USA.; aoa Shandong
University, School of Chemistry and Chemical Engineering, Jinan , Shandong ,
China.; vy Jining Medical University, Shandong Provincial Sino-US Cooperation
Research Center for Translational Medicine , Shandong , China.; qi Huazhong
Agricultural University, College of Animal Sciences and Technology, Wuhan , Hubei ,
China.; byj University of Virginia , Charlottesville , VA , USA.; apg Singapore Eye
Research Institute, Singapore National Eye Center , Singapore.; ahj New York
University Langone Medical Center, Nathan Kline Institute for Psychiatric
Research , Orangeburg , NY , USA.; ajy Pennsylvania State University, College of
Medicine , Department of Pharmacology , Pennsylvania State University Hershey
Cancer Institute , Hershey , PA , USA.; asr The Fourth Military Medical University,
Institute of Orthopaedics, Xijing Hospital , Xi'an, Shanxi , China.; dl Central
South University , Department of Pediatrics , Xiangya Hospital , Changsha, Hunan ,
China.; arl Taipei Medical University, Graduate Institute of Cancer Biology and
Drug Discovery, College of Medical Science and Technology , Taipei , Taiwan.; bnj
University of Maryland, School of Medicine , Department of Obstetrics , Gynecology
and Reproductive Sciences , Baltimore , MD , USA.; ast The Fourth Military Medical
University , Xi'an , China.; mi Geisinger Clinic, Weis Center for Research ,
Danville , PA , USA.; i Academia Sinica, Institute of Biological Chemistry , Taipei
, Taiwan.; vx Jinan University, Medical College, Division of Histology and
Embryology , Guangzhou, Guangdong , China.; oo Hangzhou Normal University ,
Department of Pharmacology , School of Medicine , Hangzhou , China.; aof Shanghai
Jiao Tong University , Department of Endocrinology and Metabolism , Affiliated
Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Key Laboratory of
Diabetes Mellitus, Shanghai Clinical Center for Diabetes , Shanghai , China.; aqk
Stanford University , Department of Radiation Oncology , Stanford , CA , USA.; bjs
University of Fribourg , Department of Medicine , Division of Physiology, Faculty
of Science , Fribourg , Switzerland.; k Academia Sinica, Institute of Molecular
Biology , Taipei , Taiwan.; afs National Institutes of Health, National Institute
on Aging, Biomedical Research Center, Laboratory of Neurosciences , Baltimore ,
MD , USA.; in Dalian Medical University , Department of Environmental and
Occupational Hygiene , Dalian , China.; fr Chinese Academy of Sciences, Shenzhen
Institutes of Advanced Technology , Guangdong , China.; boc University of
Arbor , MI , USA.; bog University of Michigan, Life Sciences Institute , Ann
Arbor , MI , USA.; ahz Northern Illinois University , Department of Biological
Sciences , DeKalb , IL , USA.; lq Fourth Military Medical University , Department
of Biochemistry and Molecular Biology , Xi'an , China.; jy Emory University ,
Department of Biology , Atlanta , GA , USA.; bxc University of Toronto, Hospital
for Sick Children Research Institute , Department of Physiology and Experimental
Medicine , Toronto , Canada.; acj Medical University of South Carolina , Department
of Cell and Molecular Pharmacology and Experimental Therapeutics , Charleston ,
SC , USA.; jw Edinburgh University, MRC Human Genetics Unit , Edinburgh , UK.; aoc
Shandong University, School of Medicine , Department of Pharmacology , Jinan,
Shandong Province , China.; atn Third Military Medical University, Research Center
for Nutrition and Food Safety, Institute of Military Preventive Medicine ,
Chongqing , China.; rn Indiana University School of Medicine , Department of
Pathology and Laboratory Medicine , Indianapolis , IN , USA.; bed University of
British Columbia , Department of Biochemistry and Molecular Biology , Vancouver,
British Columbia , Canada.; ccl Yonsei University , De artment of Biomedical
Engineering , College of Health Science , Seoul , Korea.; jc Dong-A University,
College of Medicine and Mitochondria Hub Regulation Center , Department of Anatomy
and Cell Biology , Busan , Korea.; bxy University of Ulsan College of Medicine ,
Department of Brain Science , Seoul , Korea.; acq Meiji University , Department of
Life Sciences , Kanagawa , Japan.; aiw Osaka University , Department of Genetics ,
Graduate School of Medicine, Laboratory of Intracellular Membrane Dynamics,
Graduate School of Frontier Biosciences , Osaka , Japan.; bwc University of Texas,
MD Anderson Cancer Center , Department of Hematopathology , Houston , TX , USA.; vp
Jiangsu Institute of Nuclear Medicine , Wuxi, Jiangsu , China.; cg Brigham and
Women's Hospital, Harvard Medical School , Boston , MA , USA.; aks Qingdao
University , Department of Neurology , Qingdao Municipal Hospital, School of
Medicine , Qingdao, Shandong Province , China.; fz Chinese University of Hong Kong,
Institute of Digestive Diseases , Department of Medicine and Therapeutics , State
Key Laboratory of Digestive Disease , Hong Kong.; avc Tsinghua University, State
Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University-
Peking University Joint Center for Life Sciences, School of Life Science ,
Beijing , China.; hp Columbia University, Taub Institute for Alzheimer's Disease
Research , Department of Pathology and Cell Biology , New York , NY , USA.; le
First Hospital of Jilin University , Changchun, Jilin , China.; atm Third Military
Medical University , Department of Occupational Health , Chongqing , China.; pb
Harvard Medical School , Department of Cell Biology , Boston , MA , USA.; pg
Harvard University, School of Public Health , Department of Genetics and Complex
Diseases , Boston , MA , USA.; bks University of Illinois at Chicago, College of
Medicine , Department of Ophthalmology and Visual Sciences , Chicago , IL , USA.;
gu City University of Hong Kong , Department of Biomedical Sciences , Kowloon Tong,
Hong Kong , China.; qs Icahn School of Medicine at Mount Sinai, Friedman Brain
Institute , New York , NY , USA.; bod University of Michigan , Department of
Ophthalmology and Visual Sciences , Ann Arbor , MI , USA.; auu Toronto General
Research Institute - University Health Network, Division of Advanced Diagnostics ,
Toronto, Ontario , Canada.; ll Fondazione IRCCS Istituto Nazionale dei Tumori ,
Department of Experimental Oncology and Molecular Medicine , Milan , Italy.; bre
University of Padova, Venetian Institute of Molecular Medicine , Department of
Biomedical Science , Padova , Italy.; akz Queens College of the City University of
New York , Department of Biology , Flushing , NY , USA.; adh MRC Cancer Unit,
University of Cambridge, Hutchison/MRC Research Centre , Cambridge , UK.; aqy Sun
Yat-Sen University , Department of Neurology and Stroke Center , The First
Affiliated Hospital , Guangzhou , China.; ata The People's Hospital of Hainan
Province, Medical Care Center, Haikou , Hainan , China.; a A*STAR (Agency for
Science, Technology and Research), Institute of Molecular and Cell Biology ,
Singapore.; bha University of Central Florida College of Medicine, Burnett School
of Biomedical Sciences , Orlando , FL , USA.; bcq University of Arizona ,
Department of Pharmacology and Toxicology , College of Pharmacy , Tucson , AZ ,
USA.; bzp Vancouver Prostate Centre , Vancouver, BC , Canada.; asz The People's
Hospital of Guangxi Zhuang Autonomous Region , Department of Gastroenterology
, Nanning , Guangxi , China.; bkz University of Illinois at Urbana-Champaign,
Institute for Genomic Biology , Urbana , IL , USA.; aow Shantou University Medical
College, Cancer Research Center, Shantou , Guangdong , China.; fl Chinese Academy
of Sciences, Institute of Biophysics, State Key Laboratory of Biomacromolecules ,
Beijing , China.; ajp Peking University First Hospital, Renal Division , Beijing ,
China.; fd Chinese Academy of Medical Sciences and Peking Union Medical College ,
Department of Physiology , Institute of Basic Medical Sciences , Beij ng , China.;
ajo Peking University First Hospital , Department of Internal Medicine , Beijing ,
China.; lq Fourth Military Medical University , Department of Biochemistry and
Molecular Biology , Xi'an , China.; ls Fourth Military Medical University ,
Department of Pulmonary Medicine , Xijing Hospital , Xi'an, Shaanxi Province ,
China.; ash Texas A&M University Health Science Center, Center for Translational
Cancer Research, Institute of Biosciences and Technology , Houston , TX , USA.; bcb
University of Alabama at Birmingham , Department of Pathology , Birmingham , AL ,
USA.; fe Chinese Academy of Medical Sciences and Peking Union Medical College,
Institute of Medicinal Biotechnology , Beijing , China.; vp Jiangsu Institute of
Nuclear Medicine , Wuxi, Jiangsu , China.; brq University of Pennsylvania ,
Department of Obstetrics and Gynecology ; Perelman School of Medicine ,
Philadelphia , PA , USA.; bsl University of Pittsburgh, School of Medicine ,
Department of Pharmacology and Chemical Biology , Pittsburgh , PA , USA.; ccy
Zhejiang University, Life Sciences Institute , Zhejiang , China.; fs Chinese
Academy of Sciences, South China Botanical Garden , Guangzhou , China.; ma Fudan
University, Cancer Center , Department of Integrative Oncology , Shanghai , China.;
bpq University of Newcastle, School of Biomedical Sciences and Pharmacy ,
Newcastle, NSW , Australia.; any Shandong Agricultural University, State Key
Laboratory of Crop Science , Tai'an , China.; bkp University of Houston, College of
Pharmacy, Pharmacological and Pharmaceutical Sciences , Houston , TX , USA.; bnd
University of Maryland , Department of Veterinary Medicine , College Park , MD ,
USA.; byv University of Wyoming, School of Pharmacy, College of Health Sciences ,
Laramie , WY , USA.; ccb Xijing Hospital, The Fourth Military Medical University ,
Xi'an , China.; btv University of Science and Technology of China , Anhui , China.;
cbz Xi'an Jiaotong University Health Center , Department of Pharmacology , Xi'an,
Shaanxi , China.; aop Shanghai Jiao Tong University, School of Medicine, State Key
Laboratory of Medical Genomics; Shanghai Institute of Hematology; Shanghai Rui Jin
Hospital , Shanghai , China.; aoo Shanghai Jiao Tong University, School of
Medicine, Shanghai Institute of Immunology , Shanghai , China.; ft Chinese Academy
of Sciences, State Key Laboratory of Biomacromolecules, Institute of Biophysics ,
Beijing , China.; ajt Peking University, Health Science Center , Department of
Biochemistry and Molecular Biology , Beijing , China.; bof University of Michigan ,
Department of Radiation Oncology , Division of Radiation and Cancer Biology , Ann
Arbor , MI , USA.; akr Qilu Hospital of Shandong University , Department of
Traditional Chinese Medicine , Jinan , China.; ff Chinese Academy of Medical
Sciences and Peking Union Medical College, MOH Key Laboratory of Systems Biology of
Pathogens, Institute of Pathogen Biology , Beijing , China.; bqi University of
Oklahoma Health Sciences Center , Department of Pathology , Oklahoma City , OK ,
USA.; fh Chinese Academy of Medical Sciences and Peking Union Medical College,
National Laboratory of Medical Molecular Biology, Institute of Basic Medical
Sciences , Beijing , China.; beu University of Calgary , Department of Biochemistry
and Molecular Biology , Libin Cardiovascular Institute of Alberta , Calgary, AB ,
Canada.; ccr Zhejiang University , Department of Biomedical Engineering , Qiushi
Academy for Advanced Studies , Hangzhou , China.; tf Institute of Environmental
Medicine, Division of Toxicology, Karolinska Institute , Stockholm , Sweden.; zt
Lomonosov Moscow State University, Faculty of Basic Medicine , Moscow , Russia.;
bvq University of Texas , Department of Biochemistry , Dallas , TX , USA.; bvr
University of Texas , Southwestern Medical Center, Department of Internal
Medicine , Center for Autophagy Research, Dallas , TX , USA.; pr Henan University
of Technology, College of Bioengineering , Zhengzhou, Henan Province , China.; bkt
University of Illinois at Chicago, College of Medicine , Department of Pedi trics ,
Chicago , IL , USA.; caa Virginia Commonwealth University , Department of
Microbiology and Immunology , Richmond , VA , USA.; bus University of South Florida
, Department of Pharmaceutical Science , Tampa , FL , USA.; ajp Peking University
First Hospital, Renal Division , Beijing , China.; ajr Peking University ,
Department of Medicine , Beijing , China.; aju Peking University, Institute of
Nephrology, Key Laboratory of Renal Disease, Ministry of Health of China, Key
Laboratory of Chronic Kidney Disease Prevention and Treatment, Ministry of
Education , Beijing , China.; aoe Shanghai Jiao Tong University, Bio-X Institutes ,
Shanghai , China.; aik Ohio State University , Department of Surgery , Davis Heart
and Lung Research Institute , Columbus , OH , USA.; ajt Peking University, Health
Science Center , Department of Biochemistry and Molecular Biology , Beijing ,
China.; cca Xi'an Jiaotong University Health Science Center , Department of
Biochemistry and Molecular Biology , School of Basic Medical Sciences , Shaanxi ,
China.; are Sun Yat-Sen University, State Key Laboratory of Oncology in South
China, Cancer Center , Guangzhou , China.; ey China Agricultural University,
College of Animal Science and Technology, State Key Laboratory of Animal
Nutrition , Beijing , China.; arc Sun Yat-Sen University, School of Life Sciences ,
Guangzhou , China.; gd Chinese University of Hong Kong, School of Life Science,
Centre for Cell and Developmental Biology and State Key Laboratory of
Agrobiotechnology , Sha Tin, Hong Kong.; amx Sapienza University of Rome, DAHFMO-
Section of Histology , Rome , Italy.; ajk Oxford University, Department of Oncology
, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Molecular
Oncology Laboratories , Oxford , UK.; akg Polish Academy of Sciences, Institute of
Biochemistry and Biophysics , Warsaw , Poland.; aqu Stony Brook University ,
Department of Molecular Genetics and Microbiology , Stony Brook , NY , USA.; gn
CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de
Salud Carlos III , Barcelona , Spain.; ta Institute for Research in Biomedicine
(IRB Barcelona) , The Barcelona Institute of Science and Technology, Barcelona ,
Spain.; axz Universitat de Barcelona , Departament de Bioquímica i Biologia
Molecular , Facultat de Biologia , Barcelona , Spain.; kb Emory University, School
of Medicine , Department of Microbiology and Immunology , Atlanta , GA , USA.
PY - 2016
SP - 1-222
ST - Guidelines for the use and interpretation of assays for monitoring autophagy
(3rd edition)
T2 - Autophagy
TI - Guidelines for the use and interpretation of assays for monitoring autophagy
(3rd edition)
VL - 12
ID - 10328
ER -
TY - JOUR
AB - To overcome obstacles to the regeneration of crushed dorsal root fibers at
the dorsal root entry zone, we have employed specially designed Millipore implants
coated with embryonic astrocytes to serve as a substrate for axonal growth. This
strategy was successful in promoting the growth of crushed dorsal root axons into
the grey matter of the adult mammalian spinal cord in a small number of animals.
Fiber ingrowth into the spinal cord was closely associated with the surface of the
polymer implant. In addition, unique terminal arbor malformations, not normally
present, were seen in several animals. A consistent finding was the presence of a
limited inflammatory response in regions immediately adjacent to the implant where
axons penetrate the spinal cord. Our findings suggest that providing the dorsal
root entry zone with an embryonic milieu can stimulate a limited amount of axonal
regeneration into the adult mammalian spinal cord.
AN - rayyan-553782265
AU - Kliot, M.
AU - Smith, G. M.
AU - Siegal, J. D.
AU - Silver, J.
DO - 10.1016/s0014-4886(05)80008-1
IS - 1
J2 - Exp Neurol
KW - Animals
Astrocytes/*physiology/transplantation
Axonal Transport
Female
Ganglia, Spinal/cytology/*physiology
Glial Fibrillary Acidic Protein/analysis
Horseradish Peroxidase
Male
Nerve Fibers/*physiology
*Nerve Regeneration
Rats
Spinal Cord/cytology/*physiology
Spinal Nerve Roots
Astrocytes
Polymerization
Polymers
Spinal Cord
LA - eng
N1 - Center for Neuroscience, Case Western Reserve University, School of Medicine,
Cleveland, Ohio 44106.
PY - 1990
SN - 0014-4886 (Print)
SP - 57-69
ST - Astrocyte-polymer implants promote regeneration of dorsal root fibers into
the adult mammalian spinal cord
T2 - Experimental neurology
TI - Astrocyte-polymer implants promote regeneration of dorsal root fibers into
the adult mammalian spinal cord
VL - 109
Y2 - 7
ID - 10175
ER -
TY - JOUR
AB - The plant extracts are known for their anti-inflammatory, antifungal,
antiviral and antibacterial properties. The use of plant extracts in the
preparation of bio-materials increases their biological application. In this
concern, herein reporting an eco-friendly procedure which is also a simple and cost
effective, for the synthesis of Zinc Oxide nanoparticles (ZnONPs) using Saussurea
lappa plant root (rhizome) extract as a fuel. The prepared nanoparticles were
confirmed using various characterization techniques. The Dynamic light scattering
data showed 123.5 nm particle size with -99.9 mv zeta potential which indicates
excellent stability of the particles. The peak at 541 cm(-1) in the IR spectrum is
assigned to the stretching frequency of the zinc-binding to oxygen. The X-ray
diffraction peaks confirm the close association with JCPDS Data Card No: 36-1451.
The FESEM data revealed a hexagonal wurtzite structure with a hexagonal shape of
synthesized ZnO nanoparticles. The antibacterial studies indicate the gram-negative
strains showed better inhibition activity than gram-positive strains. Among Fungal
strains, Aspergillus niger and flavus, Fusarium oxysporum, and Rhizopus oryzae
showed good inhibition activity at higher concentrations. The cytotoxic data
indicates the 5 mu g/mL of the ZnO particles showed cytotoxicity on the CHO cell
line and with IC50 value 3.164 +/- 0.8956 mu g/mL.
AN - rayyan-553780753
AU - Kolahalam, L. A.
AU - Prasad, K. R. S.
AU - Krishna, P. M.
AU - Supraja, N.
DO - 10.1016/j.heliyon.2021.e07265
IS - 6
KW - Cricetulus
Zinc
Cricetinae
Cell Line
PY - 2021
SN - 2405-8440
ST - Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles:
synthesis, characterization and its antibacterial, antifungal activities and
cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines
T2 - HELIYON
TI - Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles:
synthesis, characterization and its antibacterial, antifungal activities and
cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines
VL - 7
Y2 - 6
ID - 8775
ER -
TY - JOUR
AB - Introduction. The current direction in medicine is the creation of
immunobiological preparations to increase the effectiveness of specific
immunotherapy and immunoprophylaxis, containing immunomodulators in their
composition. These natural or synthetic substances can have a regulating effect on
the immune system. At present, water-soluble organic-inorganic polymeric materials
with nanoparticles of various chemical substances with bactericidal and
immunomodulating properties can serve as such promising compounds. The aim of the
work is to study the acute toxicity of polymer nanocomposites based on 1-vinil-
1,2,4-triazole copolymer with N-vinylpyrrolidone with silver, gold and selenium
nanoparticles and their effect on the functional state of immune system cells in
vitro. Materials and methods. The study of acute toxicity was performed on outbred
white mice. The activity of superoxide dismutase and glucose-6-phosphate
dehydrogenase were studied in guinea pig peritoneal macrophages. The study of
spontaneous and nanocomposite-induced production of pro- (interferon gamma and
tumor necrosis factor alpha) and anti-inflammatory (interleukin-4) cytokines by
blood cells was carried out using clinical material obtained from volunteers using
the ELISA method. Results. It has been established that nanocomposites with silver
and gold nanoparticles do not cause the death of white mice, their temperature
increase and body weight decrease. The average lethal dose for a nanocomposite with
selenium nanoparticles was determined as 1 gram per 1 kilogram of animal mass. It
was shown that the tested nanocomposites have a stimulating effect on the
production of cytokines by human blood cells in vitro. It was established that a
nanocomposite with selenium nanoparticles increases the activity of superoxide
dismutase and glucose-6-phosphate dehydrogenase. A comparative analysis of their
actions with the actions of commercial preparations of biological origin, with
immunomodulatory properties. Conclusion. The data obtained allow us to substantiate
the need for further research on the effects of nanocomposites based on 1-vinyl-
1,2,4-triazole copolymer with N-vinylpyrrolidone with silver, gold and selenium
nanoparticles on the macroorganism in both in vitro and in vivo conditions. © 2021
Acta Biomedica Scientifica. All rights reserved.
AN - rayyan-553780758
AU - Korytov, K. M.
AU - Dubrovina, V. I.
AU - Vityazeva, S. A.
AU - Pyatidesyatnikova, A. B.
AU - Voitkova, V. V.
AU - Prozorova, G. F.
AU - Pozdnyakov, A. S.
AU - Ivanova, A. A.
AU - Balakhonov, S. V.
DO - 10.29413/ABS.2019-4.3.13
IS - 3
KW - Acute toxicity
Blood cell
Cytokine
Glucose-6-phosphate dehydrogenase
Macrophage
Nanocomposite
Nanoparticle
Superoxide dismutase
Adjuvants, Immunologic
PY - 2019
SP - 102-109
ST - Assessment of toxic and immunoadjuvant properties of nanocomposites
T2 - Acta Biomedica Scientifica
TI - Assessment of toxic and immunoadjuvant properties of nanocomposites
85101560225&doi=10.29413%2fABS.2019-
4.3.13&partnerID=40&md5=201510333f9a21319c2ca0ec45a8962a
VL - 4
ID - 8780
ER -
TY - JOUR
AB - Purpose Previously, the family of S 100A proteins has been found to be
associated with inflammation and myelopoiesis and to be able to induce or support
myeloproliferation during chronic inflammation. Here, we studied the inflammatory
myeloid-related proteins Si 00A4, S 100A8, S 100A9 and S100Al2 in
myeloproliferative neoplasms (MPNs) in order to assess the involvement of chronic
inflammation in the pathogenesis of MPN. Methods We analyzed the S100A4, S100A8,
S100A9 and S100Al2 mRNA and protein levels in the bone marrow and circulation of
140 patients with MPN and 15 healthy controls using Western blotting, microarray-
based mRNA expression profiling and ELISA assays, respectively. In addition we
performed functional studies on the proliferation-related AKT and ERK1/2 signaling
pathways in MPN-derived granulocytes using Western blotting and proteomic analyses.
Results We found that the S100A mRNA levels were increased in MPN patient-derived
circulatory CD34(+ )cells, and that their protein expression levels were also
augmented in their granulocytes and bone marrow stroma cells, depending on the
JAK2V617F mutation allele burden. We also found that calreticulin (CALR) mutations
were related to reduced S100A8 plasma levels in primary myelofibrosis (PMF). The
S100A8 plasma levels were found to be increased in MPN, the S100A9 plasma levels in
PMF and essential thrombocythemia (ET), and the S100A12 plasma levels in
polycythemia vera (PV). These 5100A plasma levels showed a positive correlation
with the systemic inflammation marker IL-8, as well as with the numbers of
leukocytes and thrombocytes, depending on the JAK2V617F mutation status.
Additionally, we found that heterodimeric S100A8/9 can inhibit the AKT pathway in
MPN-derived granulocytes mediated by the Toll-like receptor 4 (TLR4), depending on
the CALR mutation status. Conversely, we found that blocking of the receptor for
advanced glycation end products (RAGE) increased the S100A8/9-mediated inhibition
of AKT signaling in the MPN-derived granulocytes. Moreover, we found that
heterodimeric S100A8/9 generally induced TLR4-mediated ERK1/2 dephosphorylation
proportionally to the JAK2V617F mutation allele burden. TLR4/RAGE blocking
prevented the S100A8/9-mediated inhibition of ERK1/2 phosphorylation in PV.
Conclusions From our data we conclude that the 5100A8 and S100A9 granulocyte and
plasma levels are increased in MPN patients, along with inflammation markers,
depending on their JAK2V617F mutation allele burden. We also found that SIO0A8/9-
mediated inhibition of the proliferation-related AKT and ERK1/2 signaling pathways
can be decreased by CALR mutationdependent TLR4 blocking and increased by RAGE
inhibition in MPN.
AN - rayyan-553780761
AU - Kovacic, M.
AU - Mitrovic-Ajtic, O.
AU - Beleslin-Cokic, B.
AU - Djikic, D.
AU - Suboticki, T.
AU - Diklic, M.
AU - Lekovic, D.
AU - Gotic, M.
AU - Mossuz, P.
AU - Cokic, V. P.
DO - 10.1007/s13402-018-0392-6
IS - 5
PY - 2018
SN - 2211-3428 2211-3436
SP - 541-553
ST - TLR4 and RAGE conversely mediate pro-inflammatory S100A8/9-mediated
inhibition of proliferation-linked signaling in myeloproliferative neoplasms
T2 - CELLULAR ONCOLOGY
TI - TLR4 and RAGE conversely mediate pro-inflammatory S100A8/9-mediated
inhibition of proliferation-linked signaling in myeloproliferative neoplasms
VL - 41
Y2 - 10
ID - 8783
ER -
TY - JOUR
AB - Silver nanoparticles have been used in a range of applications and although
they are already employed in medicine, there are new, promising possibilities for
their utilization. We investigated the potential of silver nanoparticles obtained
with the use of blackcurrant extract in vitro in the LPS-stimulated RAW264.7
macrophages and in vivo in the murine DSS-induced colitis model. The examined
formulations contained particles of 95 nm (Ag95) and 213 nm (Ag213) diameter. In
vitro, both formulations inhibited nitric oxide (NO) release. In vivo, the
preparations alleviated colitis as evidenced by a decreased macroscopic score and
myeloperoxidase activity (indicative of neutrophil infiltration). In both cases,
the nanoparticles of larger diameter showed better anti-inflammatory properties.
Although further tests are required, our results indicate a plausible new use of
silver nanoparticles in inflammatory bowel diseases. © 2020 Elsevier B.V.
AN - rayyan-553780763
AU - Krajewska, J. B.
AU - Długosz, O.
AU - Sałaga, M.
AU - Banach, M.
AU - Fichna, J.
DO - 10.1016/j.ijpharm.2020.119549
KW - Blackcurrant extract
Colitis
Inflammatory bowel diseases
RAW264.7 macrophages
Animals
Cell Survival
Lipopolysaccharides
Male
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Particle Size
Peroxidase
Plant Extracts
RAW 264.7 Cells
Ribes
Silver
Technology, Pharmaceutical
ascorbic acid
black currant extract
budesonide
myeloperoxidase
nitric oxide
silver nanoparticle
lipopolysaccharide
metal nanoparticle
peroxidase
plant extract
silver
animal cell
animal experiment
animal model
Article
controlled study
cytotoxicity
dextran sulfate sodium-induced colitis
enzyme activity
in vitro study
in vivo study
intestine
macrophage
male
mouse
neutrophil
nonhuman
priority journal
RAW 264.7 cell line
animal
Bagg albino mouse
cell survival
chemistry
colitis
disease model
metabolism
particle size
pharmaceutics
procedures
Nanoparticles
PY - 2020
ST - Silver nanoparticles based on blackcurrant extract show potent anti-
inflammatory effect in vitro and in DSS-induced colitis in mice
T2 - International Journal of Pharmaceutics
TI - Silver nanoparticles based on blackcurrant extract show potent anti-
inflammatory effect in vitro and in DSS-induced colitis in mice
85086734120&doi=10.1016%2fj.ijpharm.2020.119549&partnerID=40&md5=d7a9cc2197ae2d8373
ac02ac7cceeabe
VL - 585
ID - 8785
ER -
TY - JOUR
AB - The study was designed to examine the effects of silver AgNPs, 20 nm) and
titanium dioxide (Aeroxide® P25 TiO2NPs, 21 nm) nanoparticles on brain oxidative
stress parameters, its antioxidant potential and brain renin-angiotensin system
(RAS) in vivo. The analysis was performed 28 days after single dose injection of
TiO2NPs and AgNPs (10 or 5 mg/kg body weight, respectively). The AgNPs, but not
TiO2NPs, administration resulted in decreased lipid and cholesterol peroxidation.
Antioxidant enzymes gene expression and/or activity were changed differently for
TiO2NPs and AgNPs group. The TiO2NPs decreased aromatase gene expression, and
glutathione peroxidase and reductase activities. In AgNPs group the sodium
dismutase 1 and glutathione reductase mRNA levels were decreased as opposed to
their activities. Both NPs altered the expression of brain RAS genes
(angiotensinogen, renin, angiotensin I converting enzyme 1 and 2), but only TiO2NPs
caused similar changes on protein level. The expression of amyloid beta precursor
protein gene was not altered by any kind of injected NPs. The TiO2NPs were more
potent modulator of gene expression in the brain than AgNPs, despite the two times
lower dosage. These results suggest that AgNPs and TiO2NPs exposure may modulate
the brain function, but with different strength. © 2015 Elsevier Ltd.
AN - rayyan-553780764
AU - Krawczyńska, A.
AU - Lankoff, A.
AU - Herman, A. P.
AU - Oczkowski, M.
AU - Królikowski, T.
AU - Wilczak, J.
AU - Wojewódzka, M.
AU - Kruszewski, M.
DO - 10.1016/j.fct.2015.08.005
KW - Antioxidant enzymes
Brain
Inflammation
Nanoparticles
Oxidative stress
Renin-angiotensin system
Amyloid beta-Protein Precursor
Animals
Gene Expression Regulation, Enzymologic
Injections, Intravenous
Lipid Peroxidation
Male
Metal Nanoparticles
Nerve Tissue Proteins
Neuritis
Neurons
Oxidative Stress
Oxidoreductases
Particle Size
Rats, Wistar
Renin-Angiotensin System
Silver
Surface Properties
Titanium
Toxicity Tests
amyloid beta protein
angiotensinogen
aromatase
messenger RNA
oxidoreductase
renin
silver nanoparticle
sodium dismutase 1
unclassified drug
metal nanoparticle
nerve protein
silver
titanium
titanium dioxide
amyloid beta precursor protein gene
angiotensin I converting enzyme 1 gene
angiotensin I converting enzyme 2 gene
angiotensinogen gene
animal experiment
animal tissue
aromatase gene
Article
brain
controlled study
enzyme activity
genetic variability
in vivo study
inflammation
lipid peroxidation
male
neuromodulation
nonhuman
oncogene ras
oxidative stress
particle size
rat
renin angiotensin aldosterone system
renin gene
animal
antagonists and inhibitors
chemically induced
chemistry
comparative study
drug effects
enzymology
genetics
immunology
metabolism
nerve cell
neuritis
surface property
toxicity and intoxication
toxicity testing
Wistar rat
PY - 2015
SP - 96-105
ST - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
response and renin-angiotensin system in brain
TI - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
84958604797&doi=10.1016%2fj.fct.2015.08.005&partnerID=40&md5=fa2111e098521d7649ee63
a7cb0a5f0b
VL - 85
ID - 8786
ER -
TY - JOUR
AB - The study was designed to examine the effects of silver AgNPs, 20 nm) and
titanium dioxide (Aeroxide (R) P25 TiO(2)NPs, 21 nm) nanoparticles on brain
oxidative stress parameters, its antioxidant potential and brain renin-angiotensin
system (RAS) in vivo. The analysis was performed 28 days after single dose
injection of TiO(2)NPs and AgNPs (10 or 5 mg/kg body weight, respectively). The
AgNPs, but not TiO(2)NPs, administration resulted in decreased lipid and
cholesterol peroxidation. Antioxidant enzymes gene expression and/or activity were
changed differently for TiO(2)NPs and AgNPs group. The TiO(2)NPs decreased
aromatase gene expression, and glutathione peroxidase and reductase activities. In
AgNPs group the sodium dismutase 1 and glutathione reductase mRNA levels were
decreased as opposed to their activities. Both NPs altered the expression of brain
RAS genes (angiotensinogen, renin, angiotensin I converting enzyme 1 and 2), but
only TiO(2)NPs caused similar changes on protein level. The expression of amyloid
beta precursor protein gene was not altered by any kind of injected NPs. The
TiO(2)NPs were more potent modulator of gene expression in the brain than AgNPs,
despite the two times lower dosage. These results suggest that AgNPs and TiO(2)NPs
exposure may modulate the brain function, but with different strength. (C) 2015
AN - rayyan-553780765
AU - Krawczynska, A.
AU - Lankoff, A.
AU - Herman, A. P.
AU - Oczkowski, M.
AU - Krolikowski, T.
AU - Wilczak, J.
AU - Wojewodzka, M.
AU - Kruszewski, M.
DO - 10.1016/j.fct.2015.08.005
KW - Titanium
Brain
PY - 2015
SN - 0278-6915 1873-6351
SP - 96-105
ST - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
TI - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
VL - 85
Y2 - 11
ID - 8787
ER -
TY - JOUR
AB - Endophthalmitis is an inflammatory reaction of intraocular fluids or tissues.
Infectious endophthalmitis is one of the most serious complications of ophthalmic
surgery. Occasionally, infectious endophthalmitis depends on timely diagnosis and
institution of appropriate therapy. Recognition of the different clinical settings
in which endophthalmitis occurs and awareness of the highly variable presentation
it may have facilitate timely diagnosis. Biopsy of intraocular fluid/tissue is the
only method that permits reliable diagnosis and treatment. The different presenting
clinical settings, a rational approach to diagnosis (i.e., when, what, and how to
biopsy), and the treatment of infectious endophthalmitis are reviewed.
AN - rayyan-553780766
AU - Kresloff, M. S.
AU - Castellarin, A. A.
AU - Zarbin, M. A.
DO - 10.1016/S0039-6257(98)00036-8
IS - 3
KW - Acute postoperative endophthalmitis
Antibiotics
Chronic postoperative endophthalmitis
Endogenousendophthalmitis
Inflammation
Phacoanaphylactic endophthalmitis
Phacolytic glaucoma
Posttrabeculectomy endophthalmitis
Posttraumatic endophthalmitis
Sympathetic ophthalmia
Vitrectomy
Diagnostic Techniques, Ophthalmological
Endophthalmitis
Eye Infections
Humans
Ophthalmologic Surgical Procedures
Risk Factors
amikacin
aminoglycoside antibiotic agent
amphotericin b
antibiotic agent
bacitracin
beta lactam antibiotic
cefazolin
ceftazidime
cephalosporin derivative
chloramphenicol
cidofovir
ciprofloxacin
dexamethasone
gentamicin
heparin
hexachlorophene
low molecular weight heparin
metipranolol
neomycin
neosporin
ofloxacin
polysporin
povidone iodine
prednisone
rifabutin
silver protein
unindexed drug
vancomycin
antibiotic prophylaxis
bacterium culture
biopsy
clinical feature
clinical trial
drug hypersensitivity
endophthalmitis
eye photography
eye surgery
eye toxicity
fungus culture
human
infection
intravitreal drug administration
meta analysis
nonhuman
priority journal
review
risk factor
slit lamp
subconjunctival drug administration
topical drug administration
trabeculectomy
uveitis
vitrectomy
PY - 1998
SP - 193-224
ST - Endophthalmitis
T2 - Survey of Ophthalmology
TI - Endophthalmitis
0031737190&doi=10.1016%2fS0039-6257%2898%2900036-
8&partnerID=40&md5=feee3f2bb9fe1a9ae366e18084dd8f36
VL - 43
ID - 8788
ER -
TY - JOUR
AB - The sequence of biochemical and cellular responses restoring the integrity of
the subcutaneous tissue of the skin is termed as wound healing. Inflammatory
cytokine suppression and inflammatory transduction cas-cades are the major targets
for wound healing. Formulations for wound healing should promote neovasculariza-
tion and angiogenic pathways by increasing the expression of vascular endothelial
growth factor, fibroblast growth factor, and platelet-derived growth factor.
Medication used for wound healing promotes anti-inflammatory associated with anti-
bacterial action. In order to boost the effectiveness of current medical treat-
ments, the cutting-edge nanotechnology offers many novel therapies. This review
summarized and discussed wound healing, types of wounds, natural materials used for
wound healing, metallic nanoparticles and current nano drug delivery systems used
for wound healing with special emphasis on the angiogenesis role in the healing of
wounds. © 2020 Bentham Science Publishers.
AN - rayyan-553780768
AU - Krishnaswami, V.
AU - Raju, N. S.
AU - Alagarsamy, S.
AU - Kandasamy, R.
DO - 10.2174/1381612826666200701203432
IS - 36
KW - Angiogenesis
Healing
Nano-drug
Treatment
Wound
Humans
Neovascularization, Pathologic
Neovascularization, Physiologic
Platelet-Derived Growth Factor
Skin
Wound Healing
copper nanoparticle
gold nanoparticle
hydrogel
nanocarrier
nanocomposite
nanofiber
silica nanoparticle
silver nanoparticle
vasculotropin A
acute wound
Aegle marmelos
Aloe vera
angiogenesis
Article
Azadirachta indica
biocompatibility
blood vessel permeability
chronic wound
clinical evaluation
closed wound
drug formulation
Gmelina arborea
human
hypoxia
Moringa oleifera
nanoemulsion
Ocimum tenuiflorum
open wound
priority journal
tissue regeneration
wound
wound healing
neovascularization (pathology)
skin
PY - 2020
SP - 4591-4600
ST - Novel nanocarriers for the treatment of wound healing
T2 - Current Pharmaceutical Design
TI - Novel nanocarriers for the treatment of wound healing
85090844393&doi=10.2174%2f1381612826666200701203432&partnerID=40&md5=1cb170aabc5f60
621413fb74618301a5
VL - 26
ID - 8790
ER -
TY - JOUR
AB - Our aim was to evaluate effects of natural rubber membrane (NR) associated
with aqueous propolis extract (P) on the wound healing in rats burn model. The
membranes were characterized for wettability and pore presence after the membrane
preparation by casting the liquid solution in Petri dishes, at different
temperatures (27 and 60 °C). Male Wistar rats were divided into treatment groups
(n = 5 per group: control without treatment, silver sulfadiazine, NR, NRP) initiated
immediately after second-degree burn and maintained during 10 days. The NRP
membranes presented hydrophilic and porous characteristics. Macroscopic analysis
from lesions showed that all groups presented crust formation. At the end of the
experiment, the lesions treated with NRP membranes prepared at 60 °C had a higher
regression percentage indicating a faster wound healing. The biopsies showed that
the treatment with the NRP membranes induced a wound healing with collagen
production, angiogenesis, reepithelization, and a small number of inflammatory
cells, characterized by the crust detachment and the epithelium formation. In
conclusion, NRP membranes are promising as a dressing for the burns treatment since
were able to accelerate the healing process and tissue repair without the curative
switch.
AN - rayyan-553782368
AU - Krupp, T.
AU - Dos Santos, B. D.
AU - Gama, L. A.
AU - Silva, J. R.
AU - Arrais-Silva, W. W.
AU - de Souza, N. C.
AU - Américo, M. F.
AU - de Souza Souto, P. C.
DO - 10.1016/j.ijbiomac.2019.03.147
KW - Animals
*Biocompatible Materials/chemistry
Biomarkers
Biopsy
Burns/pathology/*therapy
*Membranes, Artificial
*Propolis/chemistry
Rats
*Rubber/chemistry
*Wound Healing
Wound Healing
Burns
LA - eng
N1 - Laboratório de Biologia Vascular e Histopatologia, Instituto de Ciências
Biológicas e da Saúde, Universidade Federal de Mato Grosso/UFMT, Barra do Garças,
Mato Grosso, Brazil.; Laboratório de Biologia Vascular e Histopatologia, Instituto
de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso/UFMT, Barra
do Garças, Mato Grosso, Brazil.; Laboratório de Fisiologia de Sistemas e
Toxicologia Reprodutiva, Instituto de Ciências Biológicas e da Saúde, Universidade
Federal de Mato Grosso/UFMT, Barra do Garças, Mato Grosso, Brazil.; Grupo de
Materiais Nanoestruturados, Instituto de Ciências Exatas e da Terra, Universidade
Federal de Mato Grosso/UFMT, Barra do Garças, Mato Grosso, Brazil.; Laboratório de
Parasitologia, Departamento de Morfologia, Centro de Ciências Biológicas e da
Saúde, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil.; Grupo de
Materiais Nanoestruturados, Instituto de Ciências Exatas e da Terra, Universidade
Federal de Mato Grosso/UFMT, Barra do Garças, Mato Grosso, Brazil.; Laboratório de
Fisiologia de Sistemas e Toxicologia Reprodutiva, Instituto de Ciências Biológicas
e da Saúde, Universidade Federal de Mato Grosso/UFMT, Barra do Garças, Mato Grosso,
Brazil.; Laboratório de Biologia Vascular e Histopatologia, Instituto de Ciências
Biológicas e da Saúde, Universidade Federal de Mato Grosso/UFMT, Barra do Garças,
Mato Grosso, Brazil. Electronic address: paulasouto@ufmt.br.
PY - 2019
SP - 980-988
ST - NATURAL RUBBER - PROPOLIS MEMBRANE IMPROVES WOUND HEALING IN SECOND-DEGREE
BURNING MODEL
TI - NATURAL RUBBER - PROPOLIS MEMBRANE IMPROVES WOUND HEALING IN SECOND-DEGREE
BURNING MODEL
VL - 131
Y2 - 6 y3 - 15
ID - 10275
ER -
TY - JOUR
AB - Death-associated protein kinase (DAPK) has pro-apoptotic functions and
participates in various apoptotic systems. DAPK acts as a tumor suppressor, and its
inactivation by promoter hypermethylation has been frequently observed in various
human cancers. As alterations of pro-apoptotic genes might cause instability in the
balance of cell-turnover during chronic inflammatory processes, epigenetic
silencing of DAPK might be involved in the carcinogenesis of ulcerative colitis-
associated carcinoma (UCC). To evaluate the role of DAPK in the inflammation-driven
carcinogenesis of ulcerative colitis (UC), we analyzed promoter hypermethylation
and protein expression of DAPK using methylation-specific PCR and
immunohistochemistry in 43 UCCs and paired UC-background mucosa, as well as in UC-
background mucosa of 50 patients without UCC. The frequency of methylation of DAPK
in UCCs was low (27.6%) compared to overall non-neoplastic UC-background mucosa
(48.3%; p=0.02) and sporadic colorectal carcinoma (57.4%, p=0.019). The difference
in the methylation frequency in UC-background mucosa in patients without UCC
(54.2%), compared to those with UCC (40.0%), was not significant (p=0.141).
Promoter methylation correlated significantly with decreased DAPK protein
expression (p<0.001) and severity of inflammatory activity (p=0.024). In
unmethylated UC-background mucosa, DAPK protein expression increased with activity
of UC-associated inflammation, suggesting a protective role of the pro-apoptotic
DAPK during the chronic inflammatory process of UC. Thus, inactivation of DAPK by
promoter hypermethylation might be crucial for accumulation of DNA damage in
inflamed mucosa of UC, and might therefore contribute to the initiation of the
neoplastic process and development of UC-associated carcinoma.
AN - rayyan-553782412
AU - Kuester, D.
AU - Guenther, T.
AU - Biesold, S.
AU - Hartmann, A.
AU - Bataille, F.
AU - Ruemmele, P.
AU - Peters, B.
AU - Meyer, F.
AU - Schubert, D.
AU - Bohr, U. R.
AU - Malfertheiner, P.
AU - Lippert, H.
AU - Silver, A. R.
AU - Roessner, A.
AU - Schneider-Stock, R.
DO - 10.1016/j.prp.2010.05.004
IS - 9
J2 - Pathol Res Pract
KW - Adenocarcinoma/enzymology/*genetics/pathology
Adult
Aged
Aged, 80 and over
Apoptosis Regulatory Proteins/*genetics
Calcium-Calmodulin-Dependent Protein Kinases/*genetics
Cell Transformation, Neoplastic/*genetics
Colitis, Ulcerative/complications/*genetics/pathology
Colonic Neoplasms/enzymology/*genetics/pathology
DNA Methylation
Death-Associated Protein Kinases
Female
Humans
Male
Middle Aged
Neoplasm Staging
Polymerase Chain Reaction
Promoter Regions, Genetic/genetics
Tissue Array Analysis
Young Adult
Methylation
Colitis
Colitis, Ulcerative
LA - eng
N1 - Department of Pathology, Hepatology and Infectious Diseases, Otto-von-
Guericke-University, 39120 Magdeburg, Germany. doerthe.kuester@med.ovgu.de
PY - 2010
SP - 616-24
ST - Aberrant methylation of DAPK in long-standing ulcerative colitis and
ulcerative colitis-associated carcinoma
T2 - Pathology, research and practice
TI - Aberrant methylation of DAPK in long-standing ulcerative colitis and
ulcerative colitis-associated carcinoma
VL - 206
Y2 - 9 y3 - 15
ID - 10319
ER -
TY - JOUR
AB - The article investigates hardness of a spiral of a nanotructural spiral
stent. The stent biological and biomechanical compatibility was examined in the
course of drainage of the native gullet of rats. The degree of microcirculation
disorders and inflammatory changes in the gullet wall depended on hardness of the
stent spiral.
AN - rayyan-553780771
AU - Kulikovskyi, V. F.
AU - Shkodkin, S. V.
AU - Kolobov, Y. R.
AU - Chramov, G. V.
AU - Miroshnichenko, O. V.
AU - Lyubushkin, A. V.
IS - 6
KW - Biological compatibility
Biomechanical compatibility
Drainage
Inflammation
Medical implant
Stent
Rattus
nanocoating
silver nanoparticle
animal experiment
animal model
animal tissue
Article
biocompatibility
biomechanics
cell infiltration
controlled study
elasticity
equipment design
hardness
mediastinitis
microangiopathy
muscle hypertrophy
muscle injury
nanostructural spiral stent
nonhuman
rat
stent
surgical drainage
thickness
vascular patency
Stents
PY - 2014
SP - 1457-1461
ST - Revisiting biological and biomechanical compatibility of surgical stents
TI - Revisiting biological and biomechanical compatibility of surgical stents
84911141479&partnerID=40&md5=9d1a9505551b3ec613b8341fb993f249
VL - 5
ID - 8793
ER -
TY - JOUR
AB - The mass casualties caused by the delta variant and the wave of the newer
“Omicron” variant of SARS-COV-2 in India have brought about great concern among
healthcare officials. The government and healthcare agencies are seeking effective
strategies to counter the pandemic. The application of nanotechnology and
repurposing of drugs are reported as promising approaches in the management of
COVID-19 disease. It has also immensely boomed the search for productive, re-
liable, cost-effective, and bio-assimilable alternative solutions. Since ancient
times, the traditional-ly employed Ayurvedic bhasmas have been used for diverse
infectious diseases, which are now employed as nanomedicine that could be applied
for managing COVID-19-related health anomalies. Like currently engineered metal
nanoparticles (NPs), the bhasma nanoparticles (BNPs) are also packed with unique
physicochemical properties, including multi-elemental nanocrystalline compo-sition,
size, shape, dissolution, surface charge, hydrophobicity, and multi-pathway
regulatory as well as modulatory effects. Because of these conformational and
configurational-based physico-chemical advantages, Bhasma NPs may have promising
potential to manage the COVID-19 pandemic and reduce the incidence of pneumonia-
like common lung infections in children as well as age-related inflammatory
diseases via immunomodulatory, anti-inflammatory, antiviral, and adju-vant-related
properties. © 2023 Bentham Science Publishers.
AN - rayyan-553780774
AU - Kumar, P.
AU - Jayakumar, R.
AU - Dash, M. K.
AU - Joshi, N.
DO - 10.2174/2215083808666220321145803
IS - 3
KW - Bhasma
gut microbiome
marana
nanocrystalline structure
nanoparticles
proinflammatory
transition metal
capsid protein
copper nanoparticle
cytokine
gold nanoparticle
interleukin 1beta
interleukin 2
interleukin 6
interleukin 8
iron oxide nanoparticle
metal nanoparticle
metalloprotein
nanoparticle
new drug
noradrenalin
RNA polymerase
silver nanoparticle
transition element
virus spike protein
zinc nanoparticle
adaptive immunity
antiviral activity
B lymphocyte
bioavailability
biocompatibility
biotransformation
CD4+ T lymphocyte
CD8+ T lymphocyte
child
coronavirus disease 2019
crystal structure
cytotoxicity
dendritic cell
diabetes mellitus
dissolution
elemental analysis
gene expression
half life time
homeostasis
human
humoral immunity
hydrophobicity
hyperlipidemia
incidence
inflammation
innate immunity
intestine flora
lipid peroxidation
lung injury
macrophage
metal poisoning
microbiome
nanotechnology
pandemic
particle size
pathogenesis
pharmacodynamics
physical chemistry
Review
Severe acute respiratory syndrome coronavirus 2
signal transduction
surface charge
T lymphocyte
thermodynamics
virus replication
wound healing
X ray diffraction
PY - 2023
SP - 28-43
ST - The Potential Impact of Ayurvedic Traditional Bhasma on SARS-CoV-2-Induced
Pathogenesis
T2 - Current Traditional Medicine
TI - The Potential Impact of Ayurvedic Traditional Bhasma on SARS-CoV-2-Induced
Pathogenesis
85148954485&doi=10.2174%2f2215083808666220321145803&partnerID=40&md5=7ed7c7724615cc
f9dc21603c305e81cb
VL - 9
ID - 8796
ER -
TY - JOUR
AB - Bio-neuronal led psychiatric abnormalities transpired by the loss of neuronal
structure and function (neurodegeneration), pro-inflammatory cytokines, microglial
dysfunction, altered neurotransmission, toxicants, serotonin deficiency, kynurenine
pathway, and excessively produced neurotoxic substances. These uncontrolled
happenings in the etiology of psychiatric disorders initiate further changes in
neurotransmitter metabolism, pathologic microglial, cell activation, and impaired
neuroplasticity. Inflammatory cytokines, the outcome of dysfunctional mitochondria,
dysregulation of the immune system, and under stress functions of the brain are
leading biochemical factors for depression and anxiety. Nanoscale drug delivery
platforms, inexpensive diagnostics using nanomaterials, nano-scale imaging
technologies, and ligand-conjugated nanocrystals used for elucidating the molecular
mechanisms and foremost cellular communications liable for such disorders are
highly capable features to study for efficient diagnosis and therapy of the mental
illness. These theranostic tools made up of multifunctional nanomaterials have the
potential for effective and accurate diagnosis, imaging of psychiatric disorders,
and are at the forefront of leading technologies in nanotheranostics openings field
as they can collectively and efficiently target the stimulated territories of the
cerebellum (cells and tissues) through molecular-scale interactions with higher
bioavailability, and bio-accessibility. Specifically, the nanoplatforms based
neurological changes are playing a significant role in the diagnosis of psychiatric
disorders and portraying the routes of functional restoration of mental disorders
by newer imaging tools at nano-level in all directions. Because of these
nanotherapeutic platforms, the molecules of nanomedicine can penetrate the Blood-
Brain Barrier with an increased half-life of drug molecules. The discoveries in
nanotheranostics and nanotherapeutics inbuilt unique multi-functionalities are
providing the best multiplicities of novel nanotherapeutic potentialities with no
toxicity concerns at the level of nano range. © The author(s).
AN - rayyan-553780777
AU - Kumar, R.
AU - Chhikara, B. S.
AU - Gulia, K.
AU - Chhillar, M.
DO - 10.7150/ntno.49619
IS - 3
KW - And kynurenine pathway
Microglial dysfunction
Nanotheranostics
Nanotherapeutics
Neurodegeneration
Psychiatric disorders
Blood-Brain Barrier
Humans
Inflammation
Mental Disorders
Nanomedicine
Precision Medicine
calcium channel blocking agent
catalase
corticosteroid
glutathione
gold nanoparticle
hydrogel
inflammasome
kynurenine
liposome
nanocarrier
nanocrystal
neurotransmitter
nitric oxide
prostaglandin E2
serotonin
silver nanoparticle
bioavailability
blood brain barrier
brain function
confusion
delusion
drug formulation
drug release
electrophysiology
hallucination
human
mental disease
nerve cell network
neuroapoptosis
neuroimaging
neuromodulation
neuroprotection
neuropsychiatry
neurotransmission
Review
suicidal ideation
theranostic nanomedicine
inflammation
nanomedicine
pathology
pathophysiology
PY - 2021
SP - 288-308
ST - Review of nanotheranostics for molecular mechanisms underlying psychiatric
disorders and commensurate nanotherapeutics for neuropsychiatry: The mind knockout
T2 - Nanotheranostics
TI - Review of nanotheranostics for molecular mechanisms underlying psychiatric
disorders and commensurate nanotherapeutics for neuropsychiatry: The mind knockout
85103146828&doi=10.7150%2fntno.49619&partnerID=40&md5=39aace7298e3838a51cc270c4c203
ccb
VL - 5
ID - 8799
ER -
TY - JOUR
AB - The increased biomedical applications of enzyme-mimicking nanoparticles, also
known as ‘nanozymes’ has necessitated their controlled design in terms of
physicochemical properties, and reduced toxicity. In this context, numerous
approaches have been established to manufacture nanozymes using eco-friendly
routes, however, controlled surfaces and suitable functionalization of nanozymes
need much attention to utilize their full potential. Here, serine synthesised
silver (Ag) nanozymes have been fabricated, and sequentially surface functionalized
with isonicotinic acid hydrazide (isoniazid), streptomycin, and phosphomolybdic
acid to control their nanozyme-mimicking nature, and impart biological
capabilities. Post-physicochemical characterization, all these functional Ag-
centred nanozymes were explored for their inherent peroxidase enzyme-like behavior,
and haemcompatibility. The cell viability and proliferation assessments towards
mouse RAW 264.7 macrophages confirmed the cytocompatible nature of Ag nanozymes.
Moreover, these nanozymes show anti-inflammatory potential by regulating the key
inflammatory cytokines IL-6, IL-β, and TNF-α. © 2022 Elsevier B.V.
AN - rayyan-553780778
AU - Kumawat, M.
AU - Madhyastha, H.
AU - Singh, M.
AU - Jain, D.
AU - Daima, H. K.
DO - 10.1016/j.colsurfa.2022.129294
KW - Inflammatory cytokines
Isonicotinic acid hydrazide/isoniazid
Phosphomolybdic acid
Serine
Silver nanozymes
Streptomycin
Amines
Amino acids
Macrophages
Physicochemical properties
Pyridine
Silver
interleukin 1beta
interleukin 6
isoniazid
molybdic acid
peroxidase
phosphomolybdic acid
serine
silver nanoparticle
streptomycin
unclassified drug
Cytokine expression
Isonicotinic acid
Isonicotinic acid hydrazide/isonicotinic acid hydrazide
Silver nanozyme
animal cell
Article
biocompatibility
cell proliferation
cell viability
controlled study
cytokine production
down regulation
drug cytotoxicity
homeostasis
hydrodynamics
in vitro study
macrophage
mouse
nanoengineering
nanofabrication
nonhuman
physical chemistry
RAW 264.7 cell line
surface charge
synthesis
zeta potential
Enzymes
PY - 2022
ST - Functional silver nanozymes regulate cell inflammatory cytokines expression
in mouse macrophages
T2 - Colloids and Surfaces A: Physicochemical and Engineering Aspects
TI - Functional silver nanozymes regulate cell inflammatory cytokines expression
in mouse macrophages
85132903506&doi=10.1016%2fj.colsurfa.2022.129294&partnerID=40&md5=cf2bbc62206317ea1
b153cdd4d0905bd
VL - 650
ID - 8800
ER -
TY - JOUR
AB - The smart design of nanoparticles with varying surfaces may open a new avenue
for potential biomedical applications. Consequently, several approaches have been
established for controlled synthesis to develop the unique physicochemical
properties of nanoparticles. However, many of the synthesis and functionalization
methods are chemical-based and might be toxic to limit the full potential of
nanoparticles. Here, curcumin (a plant-derived material) based synthesis of gold
(Au) nanoparticles, followed by the development of a suitable exterior corona using
isoniazid (INH, antibiotic), tyrosine (Tyr, amino acid), and quercetin (Qrc,
antioxidant), is reported. All these nanoparticles (Cur-Au, Cur-Au-INH, Cur-Au-Tyr,
and Cur-Au-Qrc) possess inherent peroxidase-mimicking natures depending on the
surface corona of respective nanoparticles, and they are found to be excellent
candidates for free radical scavenging action. The peroxidase-mimicking
nanoparticle interactions with red blood cells and mouse macrophages confirmed
their hemo- and biocompatible nature. Moreover, these surface-engineered Au
nanoparticles were found to be suitable in subsiding key pro-inflammatory cytokines
such as interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and
interleukin-1 beta (IL-1 beta). The inherent peroxidase-mimicking behavior and
anti-inflammatory potential without any significant toxicity of these nanoparticles
may open new prospects for nanomedicine.
AN - rayyan-553780780
AU - Kumawat, M.
AU - Madhyastha, H.
AU - Umapathi, A.
AU - Singh, M.
AU - Revaprasadu, N.
AU - Daima, H. K.
DO - 10.1021/acs.langmuir.1c03088
IS - 5
KW - Inflammation
PY - 2022
SN - 0743-7463 1520-5827
SP - 1877-1887
ST - Surface Engineered Peroxidase-Mimicking Gold Nanoparticles to Subside Cell
Inflammation
T2 - LANGMUIR
TI - Surface Engineered Peroxidase-Mimicking Gold Nanoparticles to Subside Cell
Inflammation
VL - 38
Y2 - 2 y3 - 8
ID - 8802
ER -
TY - JOUR
AB - BACKGROUND: Nanoscience and nanotechnology have resulted in the continuous
development of new nanomaterials with remarkable properties that make them
appealing for pharmaceutical applications. The biocompatibility of metallic
nanoparticles is of increasing interest for research scientists currently working
towards developing novel nano-based medicines, industrial chemicals, and antigens.
There is also a particular interest in using them to counter mutations that up-
regulate inflammation enhancers to produce a range of inflammation-related
pathologies. AIM: The following review discusses the anti-inflammatory mechanisms
of metallic bioconjugated (silver, gold, zinc oxide, titanium dioxide, and
selenium) nanoparticles. The current study focuses on nanoparticle manufacturing
technologies and the inflammatory response. METHODOLOGY: A thorough search was
conducted in several databases, including Scopus, Embase, Cochrane, and PubMed. The
search terms used included: Alzheimer's disease, mechanism of action,
neuroinflammation, the reaction of Mast cells to stress and neuroinflammation. The
study included all publications published in English. RESULTS: Green-synthesised
nanoparticles can suppress the NF-B and cyclooxygenase-2 pathways, preventing the
production of proinflammatory cytokines and ROS scavenging mechanisms. Metallic
nanoparticles with anti-inflammatory properties, such as stability and specific
targeting, have been briefly discussed. CONCLUSION: The current research focuses on
metallic nanoparticles employed as anti-inflammatory medication molecules, although
nanoparticles have applications in various areas (medicine, chemical engineering,
and agriculture). Nanoparticles have a large surface-to-volume ratio, which can
help them to penetrate cell membranes, and because of their solid ligand-binding
capabilities, nanoparticles have been used in the medical treatment of inflammatory
pathologies.
AN - rayyan-553781856
AU - Kurup, M.
AU - Kumar, M.
AU - Ramanathan, S.
AU - Chandra, M.
DO - 10.2174/1570163820666230718123544
J2 - Curr Drug Discov Technol
LA - eng
N1 - Department of Pharmacy, Vinayaka Missions College of Pharmacy, VMRF (DU),
Salem, Tamil Nadu, India.; Department of Pharmaceutical Chemistry, Vinayaka
Missions College of Pharmacy, VMRF (DU), Salem, Tamil Nadu, India.; Department of
Pharmacy, The Erode College of Pharmacy, Salem, Tamil Nadu, India.; Department of
Pharmacy, Vinayaka Missions College of Pharmacy, VMRF (DU), Salem, Tamil Nadu,
India.
PY - 2023
ST - The Biogenetic Synthesis of Metallic Nanoparticles and the Role They Play in
Anti-inflammatory Drug Treatment
T2 - Current drug discovery technologies
TI - The Biogenetic Synthesis of Metallic Nanoparticles and the Role They Play in
Anti-inflammatory Drug Treatment
Y2 - 7 y3 - 18
ID - 9781
ER -
TY - CHAP
AB - Nanoparticles are mainly synthesized by chemical methods that usually involve
toxic reactants as reducing agents that further produce toxic by-products, which in
turn are hazardous to the environment. During the last decade, the development of
eco-friendly alternative chemical methods based on microorganisms like bacteria or
fungi, or more recently biological molecules extracted from plants, have spurred a
lot of interest as it brings forward a solution to curb the production of toxic by-
products. The purpose of these investigations is to combine the natural biocidal
properties of nanoparticles with specific properties derived from plant extracts,
not limited to anti-inflammatory or anti-cancer properties. The development of
antibacterial/antifungal coatings based on nanoparticles exhibiting biocidal
properties offers a method to overpower multi-drug resistant bacteria. Metal oxide
nanoparticles (TiO 2 , ZnO, and MgO) and noble metal nanoparticles like Ag, Au, and
Pt have shown efficient biocidal properties against microorganisms. However, these
mechanisms are still not fully understood and moreover, depend on the type of
nanoparticles. Antibacterial activity of metal oxide nanoparticles is mainly
attributed to the production of reactive oxygen species whereas metal nanoparticles
interact with surface membranes and are capable of infiltrating into bacteria
whereupon they induce apoptosis. Here, the mechanism of preventing bacterial growth
is different from conventional antibiotics and offers a promising alternative. ©
2018 Elsevier Inc. All rights reserved.
AN - rayyan-553780782
AU - Küünal, S.
AU - Rauwel, P.
AU - Rauwel, E.
DO - 10.1016/B978-0-323-51254-1.00014-2
KW - Antibacterial coatings
Antifungal
Biocidal properties
Green synthesis
Noble metal nanoparticles
Plant extracts
Bacteria
Cell death
Drug delivery
Gold compounds
Inorganic coatings
Magnesia
Precious metals
Reducing agents
Silver compounds
TiO2 nanoparticles
Titanium dioxide
Zinc oxide
Anti-fungal
Metal nanoparticles
Plant Extracts
PY - 2018
SP - 411-446
ST - Plant extract mediated synthesis of nanoparticles
T2 - Emerging Applications of Nanoparticles and Architectural Nanostructures:
Current Prospects and Future Trends
TI - Plant extract mediated synthesis of nanoparticles
85054374489&doi=10.1016%2fB978-0-323-51254-1.00014-
2&partnerID=40&md5=2ab80e144b06620db4ca362565b2a3bf
ID - 8804
ER -
TY - JOUR
AB - Background: Continuous glucose measurements provide improved glycemic control
and may prevent hypoglycemia and long-term complications of diabetes. One of the
most promising techniques is the short-term implantation of electrochemical glucose
sensors in subcutis. However, the inflammatory reaction to these sensors may lead
to bioinstability of sensor measurements. The purpose of the present investigation
was to examine factors contributing to the observed subcutaneous inflammatory
reaction to an enzyme-based electrochemical glucose sensor for continuous glucose
measurements. The sensor biocompatibility was assessed in vitro and in vivo.
Methods: A toxicological assessment was performed on sensor materials and
leachables, and the endotoxin content of sensors was determined by a Limulus
amoebocyte lysate (LAL) test. Moreover, as a consequence of permanent penetration
of the skin by the sensor the role of bacterial migration to the tissue was
investigated. In vivo biocompatibility was investigated through histological
examination of implanted sensor membranes for 3 days in pigs. Additionally, the
effect of needle size and type (normal vs. inserter needle) on tissue trauma at
sensor insertion was evaluated, and the healing of subcutis was assessed
histologically from 3 to 14 days after removal of sensors. Results: The
toxicological assessment and the LAL test showed no concerns in a 3-day
implantation scenario, and bacterial migration to the subcutis could not be
detected. The histological examination showed that a reduction in needle size
reduced the extent of inflammation to very low levels, and that the different
sensor membranes showed similar extent and type of inflammation. Additionally, the
extent of subcutaneous tissue reaction after removal of sensors declined gradually
over time and returned to near-normal levels after 2 weeks. Conclusion: The
electrochemical enzyme-based glucose sensor for continuous glucose measurements in
subcutis is acceptable from a biocompatibility point of view. Reducing the inserter
needle in size reduces the trauma induced at sensor implantation to neglible
levels. Furthermore, the tissue reaction to the sensor returns to near-normal 2
weeks after the sensor has been removed following a 3-day implantation period. ©
Mary Ann Liebert, Inc.
AN - rayyan-553780783
AU - Kvist, P. H.
AU - Iburg, T.
AU - Aalbaek, B.
AU - Gerstenberg, M.
AU - Schoier, C.
AU - Kaastrup, P.
AU - Buch-Rasmussen, T.
AU - Hasselager, E.
AU - Jensen, H. E.
DO - 10.1089/dia.2006.8.546
IS - 5
KW - Animals
Blood Glucose
Electrochemistry
Electrodes, Implanted
Endotoxins
Female
Foreign-Body Reaction
Glucose Oxidase
Horseshoe Crabs
Materials Testing
Monitoring, Ambulatory
Subcutaneous Tissue
Swine
cellulose triacetate
dimeticone
enzyme
glucose
glucose oxidase
glutaraldehyde
platinum
polymer
silver
silver chloride
animal cell
animal experiment
animal model
animal tissue
article
biocompatibility
electrochemistry
electronic sensor
female
glucose transducer
hypoglycemia
in vitro study
in vivo study
inflammation
Limulus lysate test
nonhuman
priority journal
skin penetration
tissue reaction
wound healing
PY - 2006
SP - 546-559
ST - Biocompatibility of an enzyme-based, electrochemical glucose sensor for
short-term implantation in the subcutis
T2 - Diabetes Technology and Therapeutics
TI - Biocompatibility of an enzyme-based, electrochemical glucose sensor for
short-term implantation in the subcutis
33845673743&doi=10.1089%2fdia.2006.8.546&partnerID=40&md5=8058736920250098da20bde65
f6a42ef
VL - 8
ID - 8805
ER -
TY - JOUR
AB - Recently, palladium nanoparticles (Pd-NPs) have been shown to possess pro-
inflammatory activities. Herein, we investigated potential in vitro effects of Pd-
NPs (primary size of 1-10 nm) on the biology of neutrophils, key player cells in
inflammation. Also, the aim of this study was to evaluate the pro-inflammatory
activity of Pd-NPs using the murine air pouch model, a model previously proposed to
be used as a standard assay for testing in vivo pro-inflammatory effects of NPs.
Although the positive controls used in vitro give the expected results in all
biological functions tested, Pd-NPs do not affect the production of reactive oxygen
species and that of interleukin-1 beta (IL-1 beta), IL-6, and IL-8. Pd-NPs
moderately increase cellular adhesion of neutrophils onto human endothelial cells
and significantly increase the capacity of neutrophils to migrate and to delay
apoptosis. We conclude that Pd-NPs possess some pro-inflammatory activity in vitro
but do not attract leukocytes in vivo regardless of sex.
AN - rayyan-553780784
AU - Kwemo, P.
AU - Saafane, A.
AU - Vanharen, M.
AU - Durocher, I.
AU - Girard, D.
DO - 10.1007/s11051-020-05079-z
IS - 11
KW - Leukocytes
PY - 2020
SN - 1388-0764 1572-896X
ST - Impact of palladium nanoparticles (Pd-NPs) on the biology of neutrophils in
vitro and on leukocyte attraction in vivo
TI - Impact of palladium nanoparticles (Pd-NPs) on the biology of neutrophils in
vitro and on leukocyte attraction in vivo
VL - 22
Y2 - 11
ID - 8806
ER -
TY - JOUR
AB - Parkinson's disease (PD) is a disabling progressive neurodegenerative
disease. So far, PD's treatment remains symptomatic with no curative effects. Aside
from its blatant analgesic and antipyretic efficacy, recent studies highlighted the
endowed neuroprotective potentials of paracetamol (PCM). To this end: the present
study investigated: (1) Possible protective role of PCM against rotenone-induced
PD-like neurotoxicity in rats, and (2) the mechanisms underlying its
neuroprotective actions including cannabinoid receptors’ modulation. A dose-
response study was conducted using three doses of PCM (25, 50, and 100 mg/kg/day,
i.p.) and their effects on body weight changes, spontaneous locomotor activity,
rotarod test, tyrosine hydroxylase (TH) and α-synuclein expression, and striatal
dopamine (DA) content were evaluated. Results revealed that PCM (100 mg/kg/day,
i.p.) halted PD motor impairment, prevented rotenone-induced weight loss, restored
normal histological tissue structure, reversed rotenone-induced reduction in TH
expression and striatal DA content, and markedly decreased midbrain and striatal α-
synuclein expression in rotenone-treated rats. Accordingly, PCM (100 mg/kg/day,
i.p.) was selected for further mechanistic investigations, where it ameliorated
rotenone-induced oxidative stress, neuro-inflammation, apoptosis, and disturbed
cannabinoid receptors’ expression. In conclusion, our findings imply a multi-target
neuroprotective effect of PCM in PD which could be attributed to its antioxidant,
anti-inflammatory and anti-apoptotic activities, in addition to cannabinoid
receptors’ modulation. © 2021 Elsevier B.V.
AN - rayyan-553780787
AU - Labib, A. Y.
AU - Ammar, R. M.
AU - El-Naga, R. N.
AU - El-Bahy, A. A. Z.
AU - Tadros, M. G.
AU - Michel, H. E.
DO - 10.1016/j.intimp.2021.107431
KW - Cannabinoid receptors
Neurodegenerative diseases
Paracetamol
Parkinson's disease
Rotenone
Acetaminophen
alpha-Synuclein
Animals
Apoptosis
Corpus Striatum
Dopamine
Endocannabinoids
Male
Mesencephalon
Oxidative Stress
Parkinsonian Disorders
Rats, Wistar
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
alpha synuclein
cannabinoid 1 receptor
cannabinoid 2 receptor
cannabinoid receptor
caspase 3
cyclooxygenase 2
cytochrome c
dopamine
endocannabinoid
glutathione
malonaldehyde
messenger RNA
paracetamol
rotenone
silver chloride
tyrosine 3 monooxygenase
adult
animal experiment
animal model
animal tissue
antiapoptotic activity
apoptosis
Article
body weight change
body weight loss
chemoluminescence
controlled study
corpus striatum
drug efficacy
immunoreactivity
latency to fall
lipid peroxidation
locomotion
male
mesencephalon
motor coordination
motor dysfunction
neuroprotection
neurotoxicity
nonhuman
oxidative stress
Parkinson disease
priority journal
protein expression
rat
rotarod test
tissue structure
animal
drug effect
genetics
metabolism
parkinsonism
pathology
Wistar rat
Parkinson Disease
Rats
PY - 2021
ST - Mechanistic insights into the protective effect of paracetamol against
rotenone-induced Parkinson's disease in rats: Possible role of endocannabinoid
system modulation
T2 - International Immunopharmacology
TI - Mechanistic insights into the protective effect of paracetamol against
rotenone-induced Parkinson's disease in rats: Possible role of endocannabinoid
system modulation
85100794501&doi=10.1016%2fj.intimp.2021.107431&partnerID=40&md5=f22c067f76847ccc4ce
b53c4cdcbd124
VL - 94
ID - 8809
ER -
TY - JOUR
AB - Due to their distinctive physicochemical properties, platinum nanoparticles
(PtNPs) have emerged as a material of interest for a number of biomedical
therapeutics. However, in some instances NP exposure has been correlated to health
and safety concerns, including cytotoxicity, activation of cellular stress, and
modification to normal cell functionality. As PtNPs have induced differential
cellular responses in vitro, the goal of this study was to further characterize the
behavior and toxicological potential of PtNPs within a HepG2 liver model. This
study identified that a high PtNP dosage induced HepG2 cytotoxicity. However,
lower, subtoxic PtNP concentrations were able to elicit multiple stress responses,
secretion of proinflammatory cytokines, and modulation of insulin-like growth
factor-1 dependent signal transduction. Taken together, this work suggests that
PtNPs would not be overtly toxic for acute exposures, but sustained cellular
interactions might produce long term health consequences.
AN - rayyan-553780788
AU - Labrador-Rached, C. J.
AU - Browning, R. T.
AU - Braydich-Stolle, L. K.
AU - Comfort, K. K.
DO - 10.1155/2018/1367801
PY - 2018
SN - 1687-8191 1687-8205
ST - Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of
Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro
T2 - JOURNAL OF TOXICOLOGY
TI - Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of
Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro
VL - 2018
ID - 8810
ER -
TY - JOUR
AB - Approximately 95% of patients receiving radiotherapy (RI) will ultimately
develop radiation-induced dermatitis (RID) during or after the course of treatment,
with major consequences on quality of life and treatment outcomes. This paper
reviews the pathophysiology of RID and currently used topical products for the
prevention and treatment of RID. Although there is no consensus on the appropriate
management, recent evidence suggests that the use of topical products supports to
protect and promote tissue repair in patients with RID. Basic recommendations
include advice to wear loose clothing, using electric razors if necessary, and
avoiding cosmetic products, sun exposure or extreme temperatures. Based on
mechanisms involved and on the clinical characteristics of oncological patients,
the profile of the ideal topical product for addressing RID can be designed; it
should have limited risk of adverse events, systemic adsorption and drug-drug
interactions, should be characterized by multiple clinical activities, with a
special focus on localized pain, and should have a careful formulation as some
vehicles can block the RI beam. © 2020 Bioexcel Publishing LTD. All rights
reserved.
AN - rayyan-553780789
AU - Lacovelli, N. A.
AU - Torrente, Y.
AU - Ciuffreda, A.
AU - Guardamagna, V. A.
AU - Gentili, M.
AU - Giacomelli, L.
AU - Sacerdote, P.
DO - 10.7573/dic.2020-4-7
KW - Pain
Quality of life
Radiation-induced dermatitis
Radiotherapy
Skin toxicity
Topical treatment.
allantoin
Aloe vera extract
arginine
atorvastatin
Calendula extract
corticosteroid
epidermal growth factor
epigallocatechin gallate
fibroblast growth factor
gamma interferon inducible protein 10
glutamine
hyaluronic acid
Hypericum perforatum extract
interleukin 1alpha
interleukin 1beta
interleukin 6
interleukin 8
lanolin alcohol
mometasone furoate
neem oil
olive oil
recombinant granulocyte macrophage colony stimulating factor
sitosterol
steroid
sucralfate
sulfadiazine silver
unindexed drug
anus cancer
body mass
breast cancer
cancer radiotherapy
cytokine release
disease severity
genetic susceptibility
head and neck cancer
human
hygiene
incidence
larynx carcinoma
mouth carcinoma
nasopharynx carcinoma
nutritional status
pathophysiology
pharynx carcinoma
prevalence
psoriasis
quality of life
radiation dose
radiation exposure
radiation safety
rectum cancer
Review
risk factor
smoking
treatment duration
PY - 2020
ST - Topical treatment of radiation-induced dermatitis: Current issues and
potential solutions
T2 - Drugs in Context
TI - Topical treatment of radiation-induced dermatitis: Current issues and
potential solutions
85089454730&doi=10.7573%2fdic.2020-4-
7&partnerID=40&md5=e7ea4ef07abe61549f2129fca5e6400d
VL - 9
ID - 8811
ER -
TY - JOUR
AB - Inflammasome-derived cytokines, IL-1 beta and IL-18, and complement cascade
have been independently implicated in the pathogenesis of tuberculosis (TB)-immune
reconstitution inflammatory syndrome (TB-IRIS), a complication affecting HIV+
individuals starting antiretroviral therapy (ART). Although sublytic deposition of
the membrane attack complex (MAC) has been shown to promote NLRP3 inflammasome
activation, it is unknown whether these pathways may cooperatively contribute to
TB-IRIS. To evaluate the activation of inflammasome, peripheral blood mononuclear
cells (PBMCs) from HIV-TB co-infected patients prior to ART and at the IRIS or
equivalent timepoint were incubated with a probe used to assess active caspase-
1/4/5 followed by screening of ASC (apoptosis-associated speck-like protein
containing a CARD domain) specks as a readout of inflammasome activation by imaging
flow cytometry. We found higher numbers of monocytes showing spontaneous caspase-
1/4/5(+)ASC-speck formation in TB-IRIS compared to TB non-IRIS patients. Moreover,
numbers of caspase-1/4/5(+)ASC-speck(+) monocytes positively correlated with IL-1
beta/IL-18 plasma levels. Besides increased systemic levels of C1q and C5a, TB-IRIS
patients also showed elevated C1q and C3 deposition on monocyte cell surface,
suggesting aberrant classical complement activation. A clustering tSNE analysis
revealed TB-IRIS patients are enriched in a CD14(high)CD16(-) monocyte population
that undergoes MAC deposition and caspase-1/4/5 activation compared to TB non-IRIS
patients, suggesting complement-associated inflammasome activation during IRIS
events. Accordingly, PBMCs from patients were more sensitive to ex-vivo complement-
mediated IL-1 beta secretion than healthy control cells in a NLRP3-dependent
manner. Therefore, our data suggest complement-associated inflammasome activation
may fuel the dysregulated TB-IRIS systemic inflammatory cascade and targeting this
pathway may represent a novel therapeutic approach for IRIS or related inflammatory
syndromes. Author summary Tuberculosis (TB) associated-immune reconstitution
inflammatory syndrome (TB-IRIS) is a clinical complication affecting HIV+
individuals previously co-infected with Mycobacterium tuberculosis (Mtb), upon
antiretroviral therapy (ART) initiation. TB-IRIS is characterized by an exacerbated
inflammatory response and can be associated with high morbidity and mortality rates
in resource-limited countries with high TB prevalence. So far, there is no targeted
TB-IRIS therapy, and corticosteroids are frequently used to prevent or alleviate
IRIS related-symptoms. Here we found inflammasome activation (i.e.
caspase1/4/5(+)ASC speck complex formation) on circulating classical
CD14(high)CD16(-) monocytes may contribute to TB-IRIS immunopathology, since it
correlates with pro-inflammatory cytokine plasma levels and its decay is associated
with dampening in IRIS-related symptoms promoted by anti-inflammatory therapy. We
also found TB-IRIS monocytes display higher surface complement deposition, being
more sensitive to external complement-mediated NLRP3 inflammasome activation than
healthy control cells. In fact, complement MAC molecule C9 and caspase-1/4/5
activation were associated on classical monocytes in TB-IRIS patients, suggesting
complement-mediated inflammasome activation may lead to a positive feedback loop in
the inflammatory responses observed in TB-IRIS. Therefore, our findings support
that complement-NLRP3/ASC/caspase1/4/5 axis may be considered as a potential target
for host-directed therapy of TB-IRIS.
AN - rayyan-553780790
AU - Lage, S. L.
AU - Wong, C. S.
AU - Amaral, E. P.
AU - Sturdevant, D.
AU - Hsu, D. C.
AU - Rupert, A.
AU - Wilson, E. M. P.
AU - Qasba, S. S.
AU - Naqvi, N. S.
AU - Laidlaw, E.
AU - Lisco, A.
AU - Manion, M.
AU - Sereti, I.
DO - 10.1371/journal.ppat.1009435
IS - 3
KW - Monocytes
PY - 2021
SN - 1553-7366 1553-7374
ST - Classical complement and inflammasome activation converge in
CD14(high)CD16(-) monocytes in HIV associated TB-immune reconstitution inflammatory
syndrome
T2 - PLOS PATHOGENS
TI - Classical complement and inflammasome activation converge in
CD14(high)CD16(-) monocytes in HIV associated TB-immune reconstitution inflammatory
syndrome
VL - 17
Y2 - 3
ID - 8812
ER -
TY - JOUR
AB - It has been reported that increased levels and activity of the heme
oxygenase-1 (HO-1) protein ameliorate tissue injuries. In the present study, we
investigated the effects and mechanisms of action of gold nanoparticles (AuNPs) on
HO-1 protein expression in human vascular endothelial cells (ECs). The AuNPs
induced HO-1 protein and mRNA expression in a concentration-and time-dependent
manner. The induction was reduced by the thiol-containing antioxidants, including
N-acetylcysteine and glutathione, but not by the non-thiol-containing antioxidants
and inhibitors that block the enzymes for intracellular reactive oxygen species
generation. The AuNPs enhanced Nrf2 protein levels but did not affect Nrf2 mRNA
expression. In response to the AuNP treatment, the cytosolic Nrf2 translocated to
the nucleus, and, concomitantly, Bach1 exited the nucleus and its tyrosine
phosphorylation increased. The chromatin immunoprecipitation assay revealed that
the translocated Nrf2 bound to the antioxidant-response element located in the E2
enhancer region of the HO-1 gene promoter and acted as a transcription factor.
Although N-acetylcysteine inhibited the AuNP-induced Nrf2 nuclear translocation,
the AuNPs did not promote intracellular reactive oxygen species production or
endoplasmic reticulum stress in the ECs. Knockdown of Nrf2 expression by RNA
interference significantly inhibited AuNP-induced HO-1 expression at the protein
and mRNA levels. In summary, AuNPs enhance the levels and nuclear translocation of
the Nrf2 protein and Bach1 export/tyrosine phosphorylation, leading to Nrf2 binding
to the HO-1 E2 enhancer promoter region to drive HO-1 expression in ECs. This
study, together with our parallel findings, demonstrates that AuNPs can act as an
HO-1 inducer, which may partially contribute to their anti-inflammatory bioactivity
in human vascular ECs.
AN - rayyan-553780791
AU - Lai, T. H.
AU - Shieh, J. M.
AU - Tsou, C. J.
AU - Wu, W. B.
DO - 10.2147/IJN.S88514
KW - Humanities
Humanism
Humans
Heme Oxygenase (Decyclizing)
Heme
Nanoparticles
PY - 2015
SN - 1178-2013
SP - 5925-5939
ST - Gold nanoparticles induce heme oxygenase-1 expression through Nrf2 activation
and Bach1 export in human vascular endothelial cells
TI - Gold nanoparticles induce heme oxygenase-1 expression through Nrf2 activation
and Bach1 export in human vascular endothelial cells
VL - 10
ID - 8813
ER -
TY - JOUR
AB - Green synthesis is an eco-friendly approach of nanoparticles fabrication
gaining momentum among researches. Nanoparticles are used immensely, due to its
small size, physical properties, orientation, which can apparently change the
performance of any other material when in proximity. Antibacterial, antioxidant,
antifungal, antiviral, anti-inflammatory activities of several nanoparticles
capable of wound healing make it a appropriate module for wound dressing materials.
Silver nanoparticles (SNPs) are recognized as well established antimicrobial and
antiseptic agents, thus considering it as a promising candidate for wound healing
process and other applications. Here we report an effective and biological approach
of a novel thin film preparation based on polyvinyl alcohol (PVA) with SNPs
generated within matrix using leaf extract of Aloe vera as a bioreducing agent.
Successful incorporation of SNPs into the polymer matrix, which was confirmed by
NTA analysis, TEM, SEM. The characterization results revealed that SNPs were found
in range of 10-40 nm. Evaluation of antimicrobial activity on Escherichia coli
(ATCC 39403), Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (clinical
isolates), and Candida albicans (diploid fungus) using agar cup and disc diffusion
method confirmed effective performance of the PVA-SNPs film. Average ZOI was
observed as 3+2mm, 3+2mm, 4+1mm and 4+1mm respectively. Thin film produced is
highly biocompatible to HaCat and L929 cell lines for a defined amount and hence
can be used as wound dressing materials. The method used in this study is greener,
simple, rapid, and cost effective for producing a biocompatible film with
profoundly variable applications in food packaging and health care industries. ©
2002-2011 IEEE.
AN - rayyan-553780792
AU - Lakkakula, J.
AU - Divakaran, D.
AU - Srivastava, R.
AU - Ingle, P.
AU - Gade, A.
AU - Raut, R.
DO - 10.1109/TNB.2022.3208310
IS - 3
KW - Aloe Vera
green synthesis
polyvinyl alcohol (PVA)
silver nanoparticles (SNPs)
wound dressing
Metal Nanoparticles
Polyvinyl Alcohol
Silver
Algae
Biocompatibility
Cell culture
Escherichia coli
Film growth
Film preparation
Metal nanoparticles
Polyvinyl alcohols
Thin films
antiinfective agent
metal nanoparticle
polyvinyl alcohol
silver
<italic xmlns:ali="
Green products
Green synthesis
Medium
Polyvinyl alcohol
Silver nanoparticle
Vera</italic>
Wound
Wound dressings
Xmlns:mml="
Xmlns:xlink="
Xmlns:xsi="
RNA, Messenger
Polyglactin 910
Poly A-U
Poly I-C
PY - 2023
SP - 480-486
ST - In Situ Growth of Biocompatible Biogenic Silver Nanoparticles in Poly-Vinyl
Alcohol Thin Film Matrix
T2 - IEEE Transactions on Nanobioscience
TI - In Situ Growth of Biocompatible Biogenic Silver Nanoparticles in Poly-Vinyl
Alcohol Thin Film Matrix
85139434052&doi=10.1109%2fTNB.2022.3208310&partnerID=40&md5=405c18b72d879408b133917
bd6144cfc
VL - 22
ID - 8814
ER -
TY - JOUR
AB - Developing an efficient and cost-effective wound-healing substance to treat
wounds and regenerate skin is desperately needed in the current world. Antioxidant
substances are gaining interest in wound healing, and green-synthesized silver
nanoparticles have drawn considerable attention in biomedical applications due to
their efficient, cost-effective, and non-toxic nature. The present study evaluated
in vivo wound healing and antioxidant activities of silver nanoparticles from
Azadirachta indica (AAgNPs) and Catharanthus roseus (CAgNPs) leaf extracts in
BALB/c mice. We found rapid wound healing, higher collagen deposition, and
increased DNA and protein content in AAgNPs- and CAgNPs (1% w/w)-treated wounds
than in control and vehicle control wounds. Skin antioxidant enzyme activities
(SOD, catalase, GPx, GR) were significantly (p < 0.05) increased after 11 days
CAgNPs and AAgNPs treatment. Furthermore, the topical application of CAgNPs and
AAgNPs tends to suppress lipid peroxidation in wounded skin samples.
Histopathological images evidenced decreased scar width, epithelium restoration,
fine collagen deposition, and fewer inflammatory cells in CAgNPs and AAgNPs applied
wounds. In vitro, the free radical scavenging activity of CAgNPs and AAgNPs was
demonstrated by DPPH and ABTS radical scavenging assays. Our findings suggest that
silver nanoparticles prepared from C. roseus and A. indica leaf extracts increased
antioxidant status and improved the wound-healing process in mice. Therefore, these
silver nanoparticles could be potential natural antioxidants to treat wounds.
AN - rayyan-553781902
AU - Lakkim, V.
AU - Reddy, M. C.
AU - Lekkala, V. V. V.
AU - Lebaka, V. R.
AU - Korivi, M.
AU - Lomada, D.
IS - 5
J2 - Pharmaceutics
KW - Mice
Antioxidants
Wound Healing
LA - eng
N1 - Department of Genetics, Yogi Vemana University, Kadapa 516005, AP, India.;
Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa
516005, AP, India.; Department of Genetics, Yogi Vemana University, Kadapa 516005,
AP, India.; Department of Microbiology, Yogi Vemana University, Kadapa 516005, AP,
India.; Exercise and Metabolism Research Center, College of Physical Education and
Health Sciences, Zhejiang Normal University, Jinhua 321004, China.; Department of
Genetics, Yogi Vemana University, Kadapa 516005, AP, India.
PY - 2023
SN - 1999-4923 (Print)
ST - Antioxidant Efficacy of Green-Synthesized Silver Nanoparticles Promotes Wound
Healing in Mice
T2 - Pharmaceutics
TI - Antioxidant Efficacy of Green-Synthesized Silver Nanoparticles Promotes Wound
Healing in Mice
VL - 15
Y2 - 5 y3 - 17
ID - 9825
ER -
TY - JOUR
AB - BACKGROUND: In food toxicology, there is growing interest in studying the
impacts of foodborne nanoparticles (NPs, originating from food additives, food
supplements or food packaging) on the intestinal microbiome due to the important
and complex physiological roles of these microbial communities in host health.
Biocidal activities, as described over recent years for most inorganic and metal
NPs, could favour chronic changes in the composition and/or metabolic activities of
commensal bacteria (namely, intestinal dysbiosis) with consequences on immune
functions. Reciprocally, direct interactions of NPs with the immune system (e.g.,
inflammatory responses, adjuvant or immunosuppressive properties) may in turn have
effects on the gut microbiota. Many chronic diseases in humans are associated with
alterations along the microbiota-immune system axis, such as inflammatory bowel
diseases (IBD) (Crohn's disease and ulcerative colitis), metabolic disorders (e.g.,
obesity) or colorectal cancer (CRC). This raises the question of whether chronic
dietary exposure to inorganic NPs may be viewed as a risk factor facilitating
disease onset and/or progression. Deciphering the variety of effects along the
microbiota-immune axis may aid the understanding of how daily exposure to inorganic
NPs through various foodstuffs may potentially disturb the intricate dialogue
between gut commensals and immunity, hence increasing the vulnerability of the
host. In animal studies, dose levels and durations of oral treatment are key
factors for mimicking exposure conditions to which humans are or may be exposed
through the diet on a daily basis, and are needed for hazard identification and
risk assessment of foodborne NPs. This review summarizes relevant studies to
support the development of predictive toxicological models that account for the gut
microbiota-immune axis. CONCLUSIONS: The literature indicates that, in addition to
evoking immune dysfunctions in the gut, inorganic NPs exhibit a moderate to
extensive impact on intestinal microbiota composition and activity, highlighting a
recurrent signature that favours colonization of the intestine by pathobionts at
the expense of beneficial bacterial strains, as observed in IBD, CRC and obesity.
Considering the long-term exposure via food, the effects of NPs on the gut
microbiome should be considered in human health risk assessment, especially when a
nanomaterial exhibits antimicrobial properties.
AN - rayyan-553782298
AU - Lamas, B.
AU - Martins Breyner, N.
AU - Houdeau, E.
DO - 10.1186/s12989-020-00349-z
IS - 1
KW - Animals
*Food
Gastrointestinal Microbiome/*drug effects
Humans
Immune System/*drug effects
Immunity, Mucosal/drug effects
Inorganic Chemicals/chemistry/*toxicity
Intestinal Mucosa/drug effects
Metal Nanoparticles/chemistry/toxicity
Nanostructures/chemistry/*toxicity
Silicon Dioxide/chemistry/toxicity
LA - eng
N1 - INRAE Toxalim UMR 1331 (Research Center in Food Toxicology), Team
Endocrinology and Toxicology of the Intestinal Barrier, INRAE, Toulouse University,
ENVT, INP-Purpan, UPS, 180 Chemin de Tournefeuille, 31027, Toulouse cedex 3,
France. bruno.lamas@inrae.fr.; INRAE Toxalim UMR 1331 (Research Center in Food
Toxicology), Team Endocrinology and Toxicology of the Intestinal Barrier, INRAE,
Toulouse University, ENVT, INP-Purpan, UPS, 180 Chemin de Tournefeuille, 31027,
Toulouse cedex 3, France.; INRAE Toxalim UMR 1331 (Research Center in Food
Toxicology), Team Endocrinology and Toxicology of the Intestinal Barrier, INRAE,
Toulouse University, ENVT, INP-Purpan, UPS, 180 Chemin de Tournefeuille, 31027,
Toulouse cedex 3, France. eric.houdeau@inrae.fr.
PY - 2020
SP - 19
ST - Impacts of foodborne inorganic nanoparticles on the gut microbiota-immune
axis: potential consequences for host health
TI - Impacts of foodborne inorganic nanoparticles on the gut microbiota-immune
axis: potential consequences for host health
VL - 17
Y2 - 6 y3 - 1
ID - 10206
ER -
TY - JOUR
AB - Objectives: Using dental Ti implants has become a well-accepted and used
method for replacing missing dentition. It has become evident that in many cases
peri-implant inflammation develops. The objective was to create and evaluate the
antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can
help to prevent such processes if applied on the surface of dental implants.
Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron
Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II
procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1
cm(2) Grade 2 titanium samples. The antibacterial effect was evaluated by culturing
Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and
comparing the results to that of glass as control and of pure titanium samples.
Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved
that silver nanoparticles were present on the treated surfaces. The average
diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian
distribution, the the filling factor was 25%. Antibacterial evaluation revealed
that the nanoparticle covered samples had an antibacterial effect of 64.6% that was
statistically significant. Tests also proved that the nanoparticles are safely
anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a
silver nanoparticle layer can be an option to add antibacterial features to the
implant surface and to help in the prevention of peri-implant inflammatory
processes. Recent studies demonstrated that silver nanoparticles can induce
pathology in mammal cells, thus safe fixation of the particles is essential to
prevent them from getting into the circulation.
AN - rayyan-553780794
AU - Lampe, I.
AU - Beke, D.
AU - Biri, S.
AU - Csarnovics, I.
AU - Csik, A.
AU - Dombradi, Z.
AU - Hajdu, P.
AU - Hegedus, V.
AU - Racz, R.
AU - Varga, I.
AU - Hegedus, C.
DO - 10.2147/IJN.S197782
KW - Titanium
PY - 2019
SN - 1178-2013
SP - 4709-4721
ST - Investigation of silver nanoparticles on titanium surface created by ion
implantation technology
TI - Investigation of silver nanoparticles on titanium surface created by ion
VL - 14
ID - 8816
ER -
TY - JOUR
AB - Objectives: Using dental Ti implants has become a well-accepted and used
method for replacing missing dentition. It has become evident that in many cases
peri-implant inflammation develops. The objective was to create and evaluate the
antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can
help to prevent such processes if applied on the surface of dental implants.
Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron
Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II
procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1
cm(2) Grade 2 titanium samples. The antibacterial effect was evaluated by culturing
Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and
comparing the results to that of glass as control and of pure titanium samples.
Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved
that silver nanoparticles were present on the treated surfaces. The average
diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian
distribution, the the filling factor was 25%. Antibacterial evaluation revealed
that the nanoparticle covered samples had an antibacterial effect of 64.6% that was
statistically significant. Tests also proved that the nanoparticles are safely
anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a
silver nanoparticle layer can be an option to add antibacterial features to the
implant surface and to help in the prevention of peri-implant inflammatory
processes. Recent studies demonstrated that silver nanoparticles can induce
pathology in mammal cells, thus safe fixation of the particles is essential to
prevent them from getting into the circulation.
AN - rayyan-553781994
AU - Lampé, I.
AU - Beke, D.
AU - Biri, S.
AU - Csarnovics, I.
AU - Csik, A.
AU - Dombrádi, Z.
AU - Hajdu, P.
AU - Hegedűs, V.
AU - Rácz, R.
AU - Varga, I.
AU - Hegedűs, C.
DO - 10.2147/IJN.S197782
J2 - Int J Nanomedicine
KW - Animals
Coated Materials, Biocompatible/pharmacology
Dental Implantation/*methods
Ions
Metal Nanoparticles/*chemistry
Particle Size
Silver/*pharmacology
Stem Cells/cytology/drug effects/ultrastructure
Surface Properties
Titanium/*pharmacology
Titanium
LA - eng
N1 - Department of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry,
University of Debrecen, Debrecen, Hungary.; Department of Solid State Physics,
University of Debrecen, Debrecen, Hungary.; Hungarian Academy of Sciences,
Institute for Nuclear Research, Debrecen, Hungary.; Department of Experimental
Physics, Faculty of Science and Technology, University of Debrecen, Debrecen,
Hungary.; Hungarian Academy of Sciences, Institute for Nuclear Research, Debrecen,
Hungary.; Department of Medical Microbiology, Faculty of Medicine, University of
Debrecen, Debrecen, Hungary.; Hungarian Academy of Sciences, Institute for Nuclear
Research, Debrecen, Hungary.; Department of Orthodontics, Faculty of Dentistry,
University of Debrecen, Debrecen, Hungary.; Hungarian Academy of Sciences,
Institute for Nuclear Research, Debrecen, Hungary.; Department of Periodontology,
Faculty of Dentistry, University of Debrecen, Debrecen, Hungary.; Department of
Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of
Debrecen, Debrecen, Hungary.
PY - 2019
SP - 4709-4721
ST - Investigation of silver nanoparticles on titanium surface created by ion
T2 - International journal of nanomedicine
TI - Investigation of silver nanoparticles on titanium surface created by ion
VL - 14
ID - 9911
ER -
TY - JOUR
AB - Incisional wounds 15 mm long were induced surgically in the back skin of
young adult Wistar rats. They were sutured and used as an experimental model in the
therapeutic evaluation of daily applications of 0.5 mL of silver nitrate (SN) at
0.01, 0.1 or 1.0% w/v aqueous solution, or 0.5 g silver sulphadiazine (SSD) over a
10-day period. Control wounds received deionized water only. The silver
preparations were not toxic but SN did stain the hair and superficial layers of the
stratum corneum. The wounds remained microbiologically clean. Wounds exposed to SN
(0.1 or 1.0%) or SSD healed more rapidly than controls. From about the fourth day
of treatment, we noted a more rapid exteriorization of sutures, improved wound
closure and an earlier loss of scabs and wound debris. Silver treatment appeared to
reduce the inflammatory and granulation tissue phases of healing and enhance
epidermal repair. Silver from SN was deposited as silver sulphide in
extrafollicular hair shafts and superficial aspects of the skin and wound debris
but not at deeper levels. Silver uptake was four-fold higher in damaged skin than
in intact tissue. SSD was absorbed by intact and wounded skin but the silver did
not precipitate as silver sulphide and its localization in the tissue is not known.
Uptake of silver from SN or SSD was associated with changes in the concentrations
of zinc and calcium in the skin. Zinc levels were depressed during the inflammatory
and proliferative phases of healing and then increased. Zinc concentrations had
normalized by 10 days when wound healing was achieved. Calcium levels remained
higher than normal throughout the observation period. The mechanism of action of
silver in advancing wound healing in the rat is unclear. Its ability to reduce the
inflammatory and granulation phases of healing, and to invoke metallothionein
production and influence metal ion binding are possibly important.
AN - rayyan-553782345
AU - Lansdown, A. B.
AU - Sampson, B.
AU - Laupattarakasem, P.
AU - Vuttivirojana, A.
IS - 5
J2 - Br J Dermatol
KW - Animals
Male
Rats
Rats, Wistar
Silver Nitrate/pharmacokinetics/*pharmacology
Silver Sulfadiazine/pharmacokinetics/*pharmacology
Skin/*injuries/metabolism/pathology
Sutures
Time Factors
Acquired Immunodeficiency Syndrome
LA - eng
N1 - Department of Comparative Biology, Charing Cross and Westminster Medical
School, London, U.K.
PY - 1997
SN - 0007-0963 (Print)
SP - 728-35
ST - Silver aids healing in the sterile skin wound: experimental studies in the
laboratory rat
T2 - The British journal of dermatology
TI - Silver aids healing in the sterile skin wound: experimental studies in the
laboratory rat
VL - 137
Y2 - 11
ID - 10253
ER -
TY - JOUR
AB - Due to their characteristic physical, chemical and optical properties,
titanium dioxide and silver nanoparticles are attractive tools for use in a wide
range of applications. The use of nanoparticles for biological applications is,
however, dependent upon their biocompatibility with living cells. Because of the
importance of inflammation as a modulator of human health, the safe and efficacious
in vivo use of titanium dioxide and silver nanoparticles is inherently linked to a
favorable interaction with immune system cells. However, both titanium dioxide and
silver nanoparticles have demonstrated potential to exert immunomodulatory and
immunotoxic effects. Titanium dioxide and silver nanoparticles are readily
internalized by immune system cells, may accumulate in peripheral lymphoid organs,
and can influence multiple manifestations of immune cell activity. Although the
factors influencing the biocompatibility of titanium dioxide and silver
nanoparticles with immune system cells have not been fully elucidated, nanoparticle
core composition, size, concentration and the duration of cell exposure seem to be
important Because titanium dioxide and silver nanoparticles are widely utilized in
pharmaceutical, commercial and industrial products, it is vital that their effects
on human health and immune system function be more thoroughly evaluated. (C) 2015
AN - rayyan-553780795
AU - Lappas, C. M.
DO - 10.1016/j.fct.2015.05.015
KW - Titanium
PY - 2015
SN - 0278-6915 1873-6351
SP - 78-83
ST - The immunomodulatory effects of titanium dioxide and silver nanoparticles
TI - The immunomodulatory effects of titanium dioxide and silver nanoparticles
VL - 85
Y2 - 11
ID - 8817
ER -
TY - JOUR
AB - An in vitro cell culture approach was evaluated for its ability to provide
data pertinent to the assessment of skin irritation potential. The hypothesis of
this approach is that a direct toxic insult to the epidermal keratinocyte in vivo
may lead to release of inflammatory mediators, which are responsible for initiation
of a local primary skin irritant reaction. This paper presents data on the
cytotoxic potential of a number of structurally unrelated chemicals (chloroform, 2-
methoxyethanol, 2-butoxyethylacetate, toluene, 1- butanol, acetaldehyde, n-hexane,
sodium dodecyl sulfate, benzalkonium chloride, silver nitrate, dibutyltin
dichloride and tributyltin chloride). Cytotoxicity (neutral red uptake and
intracellular acid phosphatase activity) of a number of structurally unrelated
chemicals, representative of a wide range of skin irritation potential, was
evaluated in cultures of rat and human epidermal keratinocytes. The sensitivity of
human and rat keratinocytes to the test chemicals was very similar, irrespective of
the endpoint of cytotoxicity. The neutral red uptake assay appeared more generally
applicable to the diverse range of chemical structures represented in this study,
since not all test chemicals elicited an early increase in intracellular acid
phosphatase activity. The results were very encouraging, as a good correlation was
evident between cytotoxicity in rat keratinocytes and the degree of erythema and
oedema associated with an in vivo skin irritant response in rabbits. Keratinocyte
cytotoxicity data may provide an indication of the potential of a chemical to
induce a severe skin irritant reaction, or if a chemical is more likely to be a
marginal or non-irritant. However, the data illustrate that such assays appear
unable to discriminate correctly between more subtle classes of irritancy, such as
non-irritant, mild, moderate or severe. Available human in vivo skin irritation
data were insufficient to conclude which cell type is preferable for evaluation of
human skin irritation potential.
AN - rayyan-553780797
AU - Lawrence, J. N.
AU - Starkey, S.
AU - Dickson, F. M.
AU - Benford, D. J.
DO - 10.1016/0887-2333(96)00005-7
IS - 3
KW - Animalia
Oryctolagus cuniculus
2 methoxyethanol
acetaldehyde
acid phosphatase
benzalkonium chloride
butanol
chloroform
dibutyltin dichloride
dodecyl sulfate sodium
hexane
irritant agent
neutral red
silver nitrate
toluene
tributyltin chloride
animal cell
animal model
animal tissue
article
controlled study
drug cytotoxicity
edema
erythema
human
human cell
keratinocyte
mediator release
nonhuman
rabbit
rat
skin inflammation
skin irritation
skin toxicity
Humanities
Humanism
Humans
Rats
Keratinocytes
PY - 1996
SP - 331-340
ST - Use of human and rat keratinocyte cultures to assess skin irritation
potential
TI - Use of human and rat keratinocyte cultures to assess skin irritation
potential
0029944994&doi=10.1016%2f0887-2333%2896%2900005-
7&partnerID=40&md5=702ebfa98aa19323249574f616d9a65f
VL - 10
ID - 8819
ER -
TY - JOUR
AB - Implants and prostheses are widely used to replace damaged tissues or to
treat various diseases. However, besides the risk of bacterial or fungal infection,
an inflammatory response usually occurs. Here, recent progress in the field of
anti-inflammatory biomaterials is described. Different materials and approaches are
used to decrease the inflammatory response, including hydrogels, nanoparticles,
implant surface coating by polymers, and a variety of systems for anti-inflammatory
drug delivery. Complex multifunctional systems dealing with inflammation, microbial
infection, bone regeneration, or angiogenesis are also described. New promising
stimuli-responsive systems, such as pH- and temperature-responsive materials, are
also being developed that would enable an “intelligent” antiinflammatory response
when the inflammation occurs. Together, different approaches hold promise for
creation of novel multifunctional smart materials allowing better implant
integration and tissue regeneration. © 2020 Wiley-VCH GmbH
AN - rayyan-553780798
AU - Lebaudy, E.
AU - Fournel, S.
AU - Lavalle, P.
AU - Vrana, N. E.
AU - Gribova, V.
DO - 10.1002/adhm.202001373
IS - 1
KW - anti-inflammatory material designs
biomaterials
coatings
hydrogels
nanoparticles
Hydrogels
Nanoparticles
Polymers
Pathology
Tissue
Tissue regeneration
aceclofenac
alginic acid
alpha tocopherol
amfenac
ascorbic acid
benzoic acid
betamethasone
betamethasone dipropionate
biomaterial
carbomer
carboxymethylcellulose
catestatin
celecoxib
chitosan
chondroitin sulfate
citral
collagen
curcumin
cyclodextrin
dermatan sulfate
dexamethasone
diclofenac
dopamine
fibrin
fucoidin
gelatin
glycyrrhizic acid
heparin
hyaluronic acid
hydrogel
hydroxypropylcellulose
hydroxypropylmethylcellulose
ibuprofen
indometacin
interleukin 1
interleukin 10
interleukin 13
interleukin 1beta
interleukin 4
interleukin 6
interleukin 8
keratin
ketoprofen
levofloxacin
lidocaine
luteolin 7 glucoside
metal nanoparticle
microgel (material)
morin
Moringa oleifera extract
nanocage
nanocapsule
nanomaterial
nanoparticle
naringenin
nerolidol
pilocarpine
piroxicam
plasmid DNA
polyarginine
polycaprolactone
polyetheretherketone
polyethylene
polyglutamic acid
polylactide
polysaccharide
polystyrene
prednisolone
quercetin
resolvin D1
resveratrol
silk
silver nanoparticle
titanium
unclassified drug
polymer
Anti-inflammatories
Anti-inflammatory response
Microbial infections
Multifunctional systems
PH- and temperature-responsive
Stimuli-responsive systems
Aloe vera
arthritis
Article
biocompatibility
biodegradation
coating (procedure)
cytokine production
dermatitis
drug release
drug solubility
equipment design
honey
human
hydrophobicity
immune response
immunomodulation
implantation
inflammation
knee arthritis
macromolecule
macrophage
mouth injury
nanoencapsulation
nanofabrication
nonhuman
periodontitis
physical chemistry
porosity
priority journal
regenerative medicine
surface property
tissue engineering
tissue regeneration
wound healing
Drug delivery
PY - 2021
ST - Recent Advances in Antiinflammatory Material Design
TI - Recent Advances in Antiinflammatory Material Design
85092539540&doi=10.1002%2fadhm.202001373&partnerID=40&md5=b5199e0df15e0720bd514473b
d6f98da
VL - 10
ID - 8820
ER -
TY - JOUR
AB - Several inflammatory, infectious, and neoplastic conditions in HIV-infected
patients are distinctive or require a biopsy for diagnosis. Some differ subtly from
similar conditions seen in noninfected patients. The exanthem of acute HIV
infection cannot be diagnosed specifically on biopsy as its histologic appearance
is similar to that of other viral exanthemata. A condition that closely resembles
seborrheic dermatitis occurs in HIV-infected patients. Plasma cells, necrotic
keratinocytes, and leukocytoclasis may be present, in contrast to findings in
sporadic seborrheic dermatitis. Psoriasis and Reiter's disease also occur in HIV-
infected patients and can be specifically diagnosed as such. The category
"psoriasiform dermatitis of AIDS" thus seems to include several distinct entities
and not to be a single disease. Bacillary angiomatosis is a treatable infection
caused by a rickettsialike organism similar to Rochalimaea quintana, the agent of
trench fever. Cutaneous lesions are characterized by lobules of capillaries with
protuberant endothelial cells, neutrophils and their debris, and purplish-staining
clumps of organisms, which can be demonstrated with silver stains or electron
microscopy. An unusual reaction to atypical mycobacterial infection, in which
spindle-shaped macrophages are seen, resembles histoid leprosy. Viral skin diseases
that may challenge the dermatopathologist include unusual verrucous reactions to
chronic varicella-zoster infection and flat warts caused by the human
papillomavirus associated with epidermodysplasia verruciformis. Keratinocytes with
foamy basophilic cytoplasm may be a marker for one of these viruses, human
papillomavirus type 5. Neoplastic complications of HIV disease include Kaposi's
sarcoma and mycosis fungoides. The earliest lesions of the patch stage of Kaposi's
sarcoma show a slightly increased number of cells with small ovoid nuclei around
preexistent structures, accompanied, in some cases, by sparse infiltrates of
lymphocytes and plasma cells. Staining with antisera to type IV collagen may
highlight the vascular spaces in these early lesions. Later lesions that resemble
hemangiomas may also prove challenging and require level sections to demonstrate
the presence of spindle cells and eosinophilic globules. Although HIV is cytotoxic
to helper T cells, neoplastic proliferations of them may be seen in HIV-infected
patients. These cases of mycosis fungoides do not seem to differ from sporadically
occurring ones and occur in patients who seem not to be infected by HTLV-I.
AN - rayyan-553782402
AU - LeBoit, P. E.
IS - 1
J2 - Dermatol Clin
KW - HIV Infections/*complications
Humans
Lymphoma, AIDS-Related/pathology
Sarcoma, Kaposi/etiology/pathology
Skin/pathology
Skin Diseases/complications/*pathology
Skin Diseases, Infectious/pathology
Skin Neoplasms/etiology/pathology
HIV Infections
LA - eng
N1 - Department of Pathology, University of California School of Medicine, San
Francisco.
PY - 1992
SN - 0733-8635 (Print)
SP - 59-71
ST - Dermatopathologic findings in patients infected with HIV
T2 - Dermatologic clinics
TI - Dermatopathologic findings in patients infected with HIV
VL - 10
Y2 - 1
ID - 10309
ER -
TY - COMP
AB - The effect of silver sulfadiazine (SSD) on the proliferation of human dermal
fibroblast (HDF) was studied to determine the impact of the drug on the wound
healing process and dermal mechanical strength. Human dermal fibroblasts were
cultured to 80% confluency using DMEM with 10% FBS and viability of the cell was
estimated using neutral red assay. In addition, the 2nd degree burn wound was
prepared on the anterior part of rabbit ear skin and dressings containing SSD were
applied for 96 h. Presence of inflammatory cells and degree of re-epithelialization
were investigated in the wound. After 15 day of the induction of burn wounds, the
treated area was excised and dermal mechanical strength was quantitatively measured
with a constant speed tensiometer. SSD was found to be highly cyto-toxic in
cultured HDF cells. The topical application of SSD (2%) could control the infection
as evidenced by the lack of accumulation of inflammatory cells in histological
evaluation. Therefore, these observations suggested that the impairment of dermal
regeneration and decreased mechanical strength of dermal tissue was resulted from
the cyto-toxic effect of SSD on dermal cells. Since the decreased mechanical
strength may lead to reduction in resilience, toughness and maximum extension of
the tissue, the identification of optimum dose for SSD that limits infection while
minimizes the cyto-toxic effect may be clinically relevant.
AN - rayyan-553782074
AU - Lee, A. R.
AU - Moon, H. K.
CY - Korea (South)
DO - 10.1007/BF02980032
ET - 10
J2 - Arch Pharm Res
Animals
Biological Dressings
Burns/drug therapy/pathology
Cell Division/drug effects
Cells, Cultured
Collagen/therapeutic use
Dermis/cytology/drug effects/ultrastructure
Fibroblasts/*cytology/drug effects
Humans
Materials Testing
Rabbits
Silver Sulfadiazine/*administration & dosage/pharmacokinetics/therapeutic use
Treatment Outcome
Fibroblasts
LA - eng
N1 - College of Pharmacy, Duksung Womens University, Ssangmun-dong, Dobong-ku,
Seoul 132-714, Korea. aeri@duksung.ac.kr
PY - 2003
SN - 0253-6269 (Print)
SP - 855-60
ST - Effect of topically applied silver sulfadiazine on fibroblast cell
proliferation and biomechanical properties of the wound
T2 - Archives of pharmacal research
TI - Effect of topically applied silver sulfadiazine on fibroblast cell
proliferation and biomechanical properties of the wound
VL - 26
Y2 - 10
ID - 9987
ER -
TY - JOUR
AB - This study aimed to determine age-dependent alterations in serum cytokines,
peripheral blood mononuclear cell (PBMC) cytokine production, natural killer (NK)
cell activity, and urinary 8-epi-prostaglandin F(2α) (PGF(2α)). Nine hundred
eighty-seven healthy and nonobese subjects were divided into five age groups: 20-34
(group 1), 35-44 (group 2), 45-54 (group 3), 55-64 (group 4), and 65-80 (group 5)
years of age. After adjusting for BMI, sex, and smoking and drinking status, serum
interferon (IFN)-γ levels decreased in groups 3, 4, and 5 compared with those in
groups 1 and 2. Production of IFN-γ by unstimulated PBMCs was lower in the older
groups (groups 4 and 5) than in the younger groups (groups 1 and 3). Serum
interleukin (IL)-12 was lower in group 5 than in groups 1 and 2. In contrast, both
serum and PBMC IL-6 were higher in group 5 than in groups 1, 2, and 3. Urinary 8-
epi-PGF(2α) increased in group 3 compared with that in group 1 and further
increased in group 5. Multiple linear regression analysis revealed that serum IFN-γ
levels were negatively affected by age, and NK cell activity at a ratio of
E:T = 5:1 was positively affected by PBMC IFN-γ. This study shows the age-related
reductions in serum and PBMC IFN-γ and serum IL-12 and age-related increases in
serum and PBMC IL-6 and oxidative stress in healthy nonobese subjects.
Additionally, circulating IL-6 levels may be a better marker of the chronic low-
grade inflammatory activity associated with aging than systemic levels of high-
sensitivity C-reactive protein, TNF-α, and IL-1β.
AN - rayyan-553782317
AU - Lee, D. H.
AU - Kim, M.
AU - Lee, Y. J.
AU - Yoo, H. J.
AU - Lee, S. H.
AU - Lee, J. H.
DO - 10.1007/s12026-017-8940-0
IS - 5
J2 - Immunol Res
KW - Adult
Aged
Aged, 80 and over
Aging/*immunology
Cells, Cultured
Cytotoxicity, Immunologic
Dinoprost/*analogs & derivatives/urine
Humans
Interferon-gamma/*blood
Interleukin-12/*blood
Interleukin-6/*blood
Killer Cells, Natural/*immunology
Leukocytes, Mononuclear/*immunology
Middle Aged
Young Adult
Natural Killer T-Cells
Cytokines
LA - eng
N1 - Interdisciplinary Course of Science for Aging, Yonsei University, Seoul,
South Korea.; Research Center for Silver Science, Institute of Symbiotic Life-TECH,
Yonsei University, Seoul, South Korea.; Research Center for Silver Science,
Institute of Symbiotic Life-TECH, Yonsei University, Seoul, South Korea.; National
Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of
Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, South
Korea.; Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of
Human Ecology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South
Korea.; National Leading Research Laboratory of Clinical
Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human
Ecology, Yonsei University, Seoul, South Korea.; Department of Food and Nutrition,
Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, 50
Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.; Department of Family Practice,
National Health Insurance Corporation, Ilsan Hospital, Goyang, South Korea.;
Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei
University, Seoul, South Korea. jhleeb@yonsei.ac.kr.; National Leading Research
Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and
Nutrition, College of Human Ecology, Yonsei University, Seoul, South Korea.
jhleeb@yonsei.ac.kr.; Department of Food and Nutrition, Brain Korea 21 PLUS
Project, College of Human Ecology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu,
Seoul, 03722, South Korea. jhleeb@yonsei.ac.kr.
PY - 2017
SP - 1009-1016
ST - Age-dependent alterations in serum cytokines, peripheral blood mononuclear
cell cytokine production, natural killer cell activity, and prostaglandin F(2α)
T2 - Immunologic research
TI - Age-dependent alterations in serum cytokines, peripheral blood mononuclear
cell cytokine production, natural killer cell activity, and prostaglandin F(2α)
VL - 65
Y2 - 10
ID - 10225
ER -
TY - JOUR
AB - Cyclooxygenase-2 (COX-2) is a useful biomarker of the inflammatory potential
of biomaterials in vitro. In this study we investigate the effects of soluble
extracts from 3 selected root canal sealers (AH26, Sealapex, and N2 Universal) on
COX-2 mRNA expression in cultured murine macrophage cells. Root canal sealers and
the addition of lipopolysaccharide (LPS) both produced significant increases in
COX-2 mRNA expression in RAW 264.7 macrophages. In addition, both Sealapex and N2
Universal produced a synergistic 6- to 8-fold increase in COX-2 mRNA expression,
whereas AH26 did not demonstrate synergy with LPS. These results suggest that LPS
and certain root canal sealers have a synergistic effect on the inflammatory
responses of macrophages. Under the conditions of this in vitro study, the results
suggest that one potential mechanism of periapical inflammatory reactions might be
the synergistic effects of certain root canal sealers on LPS-induced COX-2
expression by macrophage cells.
AN - rayyan-553782260
AU - Lee, D. H.
AU - Kim, N. R.
AU - Lim, B. S.
AU - Lee, Y. K.
AU - Hwang, K. K.
AU - Yang, H. C.
DO - 10.1016/j.joen.2007.07.042
IS - 11
J2 - J Endod
KW - Animals
Bismuth/toxicity
Calcium Hydroxide/toxicity
Cell Line
Cell Survival/*drug effects
Cyclooxygenase 2/*biosynthesis
Drug Combinations
Drug Synergism
Macrophages/*drug effects/*enzymology
Mice
RNA, Messenger/biosynthesis
Salicylates/toxicity
Silver/toxicity
Titanium/toxicity
Macrophages
Prostaglandin-Endoperoxide Synthases
LA - eng
N1 - Department of Dental Biomaterials Science and Dental Research Institute,
School of Dentistry, Seoul National University, Seoul, Korea.
PY - 2007
SN - 0099-2399 (Print)
SP - 1329-33
ST - Effects of root canal sealers on lipopolysaccharide-induced expression of
cyclooxygenase-2 mrna in murine macrophage cells
TI - Effects of root canal sealers on lipopolysaccharide-induced expression of
cyclooxygenase-2 mrna in murine macrophage cells
VL - 33
Y2 - 11
ID - 10170
ER -
TY - JOUR
AB - The aim of this study was to investigate the impact of consuming dairy yogurt
supplemented with rhamnogalacturonan (RG), a polysaccharide from the peel of the
Korean citrus hallabong, on natural killer (NK) cell activity and circulating
cytokine levels. A randomized, double-blind, placebo-controlled study was conducted
on 120 nondiabetic and nonobese subjects. Over an eight-week period, the test group
consumed one pack (150 mL) of dairy yogurt containing 50 mg of probiotics and 100
mg of hallabong peel polysaccharide (60% RG) each day, whereas the placebo group
consumed the same product without the hallabong peel supplement. NK cell activity
(%) was measured based on the ratios of the effector cells (E; peripheral blood
mononuclear cells, PBMCs) from each participant relative to the target cells (T;
K562 cells) at E : T ratios of 10 : 1, 5 : 1, 2.5 : 1, or 1.25 : 1. NK cell
activities under all assay conditions and interleukin (IL)-12 and interferon (IFN)-
gamma levels were significantly increased in the test group at eight weeks compared
to the baseline values, whereas the placebo group showed a significant increase
only in NK cell activity at E : T = 1.25 : 1. The test group had significantly
greater increases in the changes in serum NK cell activity at the E : T ratios of
10 : 1, 5 : 1, and 2.5 : 1 and in the increases in IL-12 and IFN-gamma levels than
were observed in the placebo group, after adjusting for baseline values. After
eight weeks of treatment, significant reductions were found in IL-6 and IL-1 beta
levels in both the placebo and test groups. The daily consumption of dairy yogurt
supplemented with RG, a polysaccharide from the peel of the Korean citrus
hallabong, enhanced NK cell function and attenuated pro-inflammatory cytokine
levels.
AN - rayyan-553780803
AU - Lee, M. H.
AU - Kim, M.
AU - Kwak, J. H.
AU - Chang, D. H.
AU - Yu, W. K.
AU - Lee, S. H.
AU - Lee, J. H.
DO - 10.1039/c5fo01103e
IS - 6
Citrus
PY - 2016
SN - 2042-6496 2042-650X
SP - 2833-2839
ST - Consumption of dairy yogurt with the polysaccharide rhamnogalacturonan from
the peel of the Korean citrus hallabong enhances immune function and attenuates the
T2 - FOOD & FUNCTION
TI - Consumption of dairy yogurt with the polysaccharide rhamnogalacturonan from
the peel of the Korean citrus hallabong enhances immune function and attenuates the
VL - 7
ID - 8825
ER -
TY - JOUR
AB - Alzheimer disease (AD) is a neurodegenerative disorder characterized by
excessive accumulation of amyloid-beta peptide (Aβ) and progressive loss of
neurons. Therefore, the inhibition of Aβ-induced neurotoxicity is a potential
therapeutic approach for the treatment of AD. Ecklonia cava is an edible brown
seaweed, which has been recognized as a rich source of bioactive derivatives,
mainly phlorotannins. In this study, phlorotannins including eckol, dieckol, 8,8'-
bieckol were used as potential neuroprotective candidates for their anti-apoptotic
and anti-inflammatory effects against Aβ(25-35)-induced damage in PC12 cells. Among
the tested compounds, dieckol showed the highest effect in both suppressing
intracellular oxidative stress and mitochondrial dysfunction and activation of
caspase family. Three phlorotannins were found to inhibit TNF-α, IL-1β and PGE₂
production at the protein levels. These result showed that the anti-inflammatory
properties of our compounds are related to the down-regulation of proinflammatory
enzymes, iNOS and COX-2, through the negative regulation of the NF-κB pathway in
Aβ(25-35)-stimulated PC12 cells. Especially, dieckol showed the strong anti-
inflammatory effects via suppression of p38, ERK and JNK. However, 8,8'-bieckol
markedly decreased the phosphorylation of p38 and JNK and eckol suppressed the
activation of p38. Therefore, the results of this study indicated that dieckol from
E. cava might be applied as a drug candidate for the development of new generation
therapeutic agents against AD.
AN - rayyan-553782107
AU - Lee, S.
AU - Youn, K.
AU - Kim, D. H.
AU - Ahn, M. R.
AU - Yoon, E.
AU - Kim, O. Y.
AU - Jun, M.
DO - 10.3390/md17010007
IS - 1
J2 - Mar Drugs
KW - Alzheimer Disease/*drug therapy/pathology
Amyloid beta-Peptides/*antagonists & inhibitors
Animals
Apoptosis/drug effects
Benzofurans/*pharmacology/therapeutic use
Cyclooxygenase 2/metabolism
Dioxins/*pharmacology/therapeutic use
Down-Regulation
Drug Evaluation, Preclinical
MAP Kinase Signaling System/drug effects
Neuroprotective Agents/*pharmacology/therapeutic use
Nitric Oxide Synthase Type II/metabolism
PC12 Cells
Peptide Fragments/*antagonists & inhibitors
Phaeophyta/*chemistry
Rats
Seaweed/*chemistry
LA - eng
N1 - Department of Food Science and Nutrition, College of Health Sciences, Dong-A
University, 37, Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, Korea.
lse2340@naver.com.; Center for Silver-Targeted Biomaterials, Brain Busan 21 Plus
Program, Graduate School, Dong-A University, Nakdong-daero 550 beon-gil, Saha-gu,
Busan 49315, Korea. lse2340@naver.com.; Department of Food Science and Nutrition,
College of Health Sciences, Dong-A University, 37, Nakdong-daero 550 beon-gil,
Saha-gu, Busan 49315, Korea. kjyoun@dau.ac.kr.; Department of Medicinal
Biotechnology, College of Health Sciences, Dong-A University, 37, Nakdong-daero 550
beon-gil, Saha-gu, Busan 49315, Korea. mose79@dau.ac.kr.; Institute of Convergence
Bio-Health, Dong-A University, Busan 49315, Korea. mose79@dau.ac.kr.; Department of
Food Science and Nutrition, College of Health Sciences, Dong-A University, 37,
Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, Korea. mokuren@dau.ac.kr.; Center
for Silver-Targeted Biomaterials, Brain Busan 21 Plus Program, Graduate School,
Dong-A University, Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, Korea.
mokuren@dau.ac.kr.; Department of Food Science and Nutrition, College of Health
Sciences, Dong-A University, 37, Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315,
Korea. ejyoon@dau.ac.kr.; Department of Food Science and Nutrition, College of
Health Sciences, Dong-A University, 37, Nakdong-daero 550 beon-gil, Saha-gu, Busan
49315, Korea. oykim@dau.ac.kr.; Center for Silver-Targeted Biomaterials, Brain
Busan 21 Plus Program, Graduate School, Dong-A University, Nakdong-daero 550 beon-
gil, Saha-gu, Busan 49315, Korea. oykim@dau.ac.kr.; Department of Food Science and
Nutrition, College of Health Sciences, Dong-A University, 37, Nakdong-daero 550
beon-gil, Saha-gu, Busan 49315, Korea. mjun@dau.ac.kr.; Center for Silver-Targeted
Biomaterials, Brain Busan 21 Plus Program, Graduate School, Dong-A University,
Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, Korea. mjun@dau.ac.kr.; Institute
of Convergence Bio-Health, Dong-A University, Busan 49315, Korea. mjun@dau.ac.kr.
PY - 2018
ST - Anti-Neuroinflammatory Property of Phlorotannins from Ecklonia cava on Aβ(25-
35)-Induced Damage in PC12 Cells
T2 - Marine drugs
TI - Anti-Neuroinflammatory Property of Phlorotannins from Ecklonia cava on Aβ(25-
35)-Induced Damage in PC12 Cells
VL - 17
Y2 - 12 y3 - 22
ID - 10018
ER -
TY - JOUR
AB - Clinical management of Clostridium difficile infection is still far from
satisfactory as bacterial spores are resistant to many chemical agents and physical
treatments. Certain types of nanoparticles have been demonstrated to exhibit anti-
microbial efficacy even in multi-drug resistance bacteria. However, most of these
studies failed to show biocompatibility to the mammalian host cells and no study
has revealed in vivo efficacy in C. difficile infection animal models. The spores
treated with 500 mu g/mL Fe3-delta O4 nanoparticles for 20 minutes, 64% of the
spores were inhibited from transforming into vegetative cells, which was close to
the results of the sodium hypochlorite-treated positive control. By cryo-electron
micro-tomography, we demonstrated that Fe3-delta O4 nanoparticles bind on spore
surfaces and reduce the dipicolinic acid (DPA) released by the spores. In a C.
difficile infection animal model, the inflammatory level triple decreased in mice
with colonic C. difficile spores treated with Fe3-delta O4 nanoparticles.
Histopathological analysis showed a decreased intense neutrophil accumulation in
the colon tissue of the Fe3-delta O4 nanoparticle-treated mice. Fe3-delta O4
nanoparticles, which had no influence on gut microbiota and apparent side effects
in vivo, were efficacious inhibitors of C. difficile spore germination by attacking
its surface and might become clinically feasible for prophylaxis and therapy.
AN - rayyan-553780808
AU - Lee, W. T.
AU - Wu, Y. N.
AU - Chen, Y. H.
AU - Wu, S. R.
AU - Shih, T. M.
AU - Li, T. J.
AU - Yang, L. X.
AU - Yeh, C. S.
AU - Tsai, P. J.
AU - Shieh, D. B.
DO - 10.1038/s41598-017-08387-y
KW - Oxalic Acid
Inflammation
Iron
Norisoprenoids
PY - 2017
SN - 2045-2322
ST - Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore
Germination and Attenuated Local and Systemic Inflammation
TI - Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore
Germination and Attenuated Local and Systemic Inflammation
VL - 7
Y2 - 8 y3 - 15
ID - 8829
ER -
TY - JOUR
AB - Transgenic mice expressing a mutated human Cu/Zn superoxide dismutase (SOD1)
gene develop a motor neuron disease similar to familial amyotrophic lateral
sclerosis (FALS). While the histopathology and the inflammatory reactions in the
spinal cord of these mice are well described, their spatiotemporal extension into
brain areas and the relationship between degenerative and inflammatory events
remain obscure. In the present study, we investigated the time course and extent of
degenerative changes and inflammatory reactions in the CNS during progression of
the disease in a transgenic FALS model, the SOD1-G93A mouse with histological and
immunohistochemical methods. Compared to non-transgenic littermates, the SOD1-G93A
transgenics developed widespread degeneration in both motor and extra-motor regions
up to telencephalic regions, including the cerebral cortex but sparing distinct
regions like the striatum and hippocampus. We provide evidence that these
degenerative processes are accompanied by intense inflammatory reactions in the
brain, which spatiotemporally correlate with degeneration and comprise besides
strong astro- and microgliotic reactions also an influx of peripheral immune cells
such as T-lymphocytes and dendritic cells. Both degeneration and inflammatory
reactions spread caudocranially, starting at 2 months in the spinal cord and
reaching the telencephalon at 5 months of age. Since the corticospinal tract lacked
any signs of degeneration, we conclude that the upper and the lower motor neurons
degenerate independently of each other. (c) 2006 Elsevier B.V. All rights reserved.
AN - rayyan-553780811
AU - Leichsenring, A.
AU - Linnartz, B.
AU - Zhu, X. R.
AU - Lubbert, H.
AU - Stichel, C. C.
DO - 10.1016/j.brainres.2006.04.029
KW - Motor Neuron Disease
Amyotrophic Lateral Sclerosis
Mice
PY - 2006
SN - 0006-8993 1872-6240
SP - 180-195
ST - Ascending neuropathology in the CNS of a mutant SOD1 mouse model of
T2 - BRAIN RESEARCH
TI - Ascending neuropathology in the CNS of a mutant SOD1 mouse model of
VL - 1096
Y2 - 6 y3 - 22
ID - 8832
ER -
TY - JOUR
AB - Metals are components of a variety of biomaterials used in orthopedic and
dental appliances; however, their biocompatibility with the surrounding tissues is
not completely understood. Monocytes are important immune cells that respond to
inflammatory stimuli by rapidly producing a variety of inflammatory proteins.
Regulation of this response often involves activation of the transcription factor
NFκB. The current study was designed to determine whether monocyte activation of
NFκB in response to bacterial lipopolysaccharide (LPS) is affected by pretreatment
with metal ions. Concentrations of metal ions that affected cell number after 24 h
of exposure were first determined. Then THP-1 human monocytes were cultured for 2 h
in media containing metal ions at concentrations below levels that altered cell
growth. Parallel cultures were treated with 10 μg/mL Escherichia coli LPS, and all
samples were cultured an additional 2 h. Nuclear proteins were extracted and
normalized amounts were incubated with [32p]-end-labeled NFκB consensus
oligonucleotide. NFκB-DNA complexes were identified and quantified by
electrophoretic mobility shift analysis. The extent of NFκB-DNA complex formation
after metal ion pretreatment with or without LPS induction was compared to no
treatment or LPS-only treated controls. Finally, LPS-induced IL1β secretion was
measured from palladium-treated and control cells. Concentrations were identified
for each metal ion (Ag+, Co 2+ Cu2+, Hg2+, Ni2+, and Pd 2+) that did not reduce
cell number after 24 h of exposure (ranging from 5 μM for Ag+ and Hg2+ to 200 μM
for Ni 2+). Exposures of 2 h at these concentrations did not alter cell morphology,
staining with trypan blue, or cell number. LPS exposure had no effect on cell
number with or without metal ions after 2 h. When metal treatment alone was
assessed, none of the metal ions had a significant effect on NFκB-DNA binding.
However, pretreatment with Co2+, Ni 2+, Ag1+, Hg2+, and Pd2+ significantly
decreased NFκB-DNA binding by 40-70% versus LPS alone. Only Cu2+ had no effect on
LPS-induced NFκB-DNA complex formation. Pd2+ lowered, but did not abolish, IL1β
secretion at concentrations comparable to those that altered NFκB-DNA binding.
These results suggest that many commonly used metals alter monocyte function at
concentrations that are not overtly toxic, and that protein levels controlled in
part by NFκB also may be altered. © 2003 Wiley Periodicals, Inc.
AN - rayyan-553780813
AU - Lewis, J. B.
AU - Wataha, J. C.
AU - Randol, T. M.
AU - McCloud, V. V.
AU - Lockwood, P. E.
DO - 10.1002/jbm.a.10169
IS - 3
KW - Dental materials
Heavy metals
Inflammation
NFκB
Biocompatibility
Biomaterials
Cell culture
Cells
Flammability
Heavy ions
Immunology
bacterium lipopolysaccharide
biomaterial
cobalt
copper ion
heavy metal
interleukin 1beta
lipopolysaccharide
mercury
metal ion
nickel
nuclear protein
oligonucleotide
palladium
phosphorus 32
protein
silver
trypan blue
Metal ions
article
biocompatibility
bone prosthesis
cell activation
cell count
cell function
cell growth
cell structure
controlled study
cytokine release
DNA protein complex
Escherichia coli
gel mobility shift assay
human
human cell
inflammation
monocyte
tooth filling
Polysaccharides
Monocytes
Lipopolysaccharides
PY - 2003
SP - 868-875
ST - Metal ions alter lipopolysaccharide-induced NFκB binding in monocytes
T2 - Journal of Biomedical Materials Research - Part A
TI - Metal ions alter lipopolysaccharide-induced NFκB binding in monocytes
0346754910&doi=10.1002%2fjbm.a.10169&partnerID=40&md5=f0542b5b6ce3633f0ef1b747f0876
243
VL - 67
ID - 8834
ER -
TY - JOUR
AB - Aim: White tip silver needle, a slightly fermented white tea, is abundant in
flavonoids, and it has great significance in terms of
D-galactose/lipopolysaccharide-induced aging in mice. Methods: We analyzed the
antioxidant capacity of white tip silver needle flavonoids (WTSNF) in vitro,
assessed the effects of WTSNF on organ indexes, pathological changes, liver
function indexes, biochemical indicators, molecular biological indicators, and
genes related to oxidation and inflammation. Results: Ultra-high performance liquid
chromatography-tandem mass spectrometry results showed that WTSNF contained
baicalin, kaempferol, kaempferide, quercetin, isorhamnetin, lespenephryl, and
rutin. WTSNF showed strong scavenging ability for both 1,1-diphenyl-2-
picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)
diammonium salt (ABTS) free radicals. Pathological analysis results showed that
WTSNF reduced liver, kidney, and lung damage in mice with induced aging. In the
serum and liver tissue, WTSNF effectively increased the antioxidant-related levels
of superoxide dismutase, catalase, glutathione peroxidase, glutathione, and total
antioxidant capacity and reduced the levels of aspartate aminotransferase, alanine
aminotransferase, malondialdehyde and nitric oxide. WTSNF also reduced the
inflammation-related levels of interleukin-6, interleukin-1 beta, tumor necrosis
factor alpha (TNF alpha), and interferon gamma (IFN-gamma) and increased the levels
of interleukin-10 and interleukin-12. Furthermore, WTSNF upregulated the mRNA
expression levels of cupro-zinc superoxide dismutase, manganese superoxide
dismutase, catalase, glutathione peroxidase, interleukin-10, neuronal nitric oxide
synthase, endothelial nitric oxide synthase, nuclear factor erythroid 2-related
factor, heme oxygenase 1, NAD(P)H dehydrogenase [quinone] 1, nuclear factor of
kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (I kappa B-
alpha), and thioredoxin, while it downregulated the mRNA expression levels of
interleukin-6, interleukin-18, interleukin-1 beta, TNF alpha, IFN-gamma, inducible
nitric oxide synthase, cyclooxygenase-2, and nuclear factor kappa-light chain-
enhancer of activated B cells (NF-kappa B). Conclusion: WTSNF is a high-quality
natural product with antioxidative and anti-inflammatory properties that can
inhibits D-galactose/lipopolysaccharide-induced aging in mice.
AN - rayyan-553780814
AU - Li, C.
AU - He, J. C.
AU - Yang, Y.
AU - Gou, Y. T.
AU - Wang, Z. Y.
AU - Chen, H.
AU - Zhao, X.
DO - 10.2147/DDDT.S304885
KW - European Continental Ancestry Group
Flavonoids
Fermentation
PY - 2021
SN - 1177-8881
SP - 1441-1457
ST - White Tip Silver Needle (Slightly Fermented White Tea) Flavonoids Help
Prevent Aging via Antioxidative and Anti-Inflammatory Effects
T2 - DRUG DESIGN DEVELOPMENT AND THERAPY
TI - White Tip Silver Needle (Slightly Fermented White Tea) Flavonoids Help
Prevent Aging via Antioxidative and Anti-Inflammatory Effects
VL - 15
ID - 8835
ER -
TY - JOUR
AB - A model to examine the in vivo relationship of acute phase serum amyloid A
(SAA) to spleen cholesterol mobilisation was devised. Reticuloendothelial cells in
vivo were loaded with a known quantity of cholesterol (1.5 mg) by infusing
fragmented red blood cell membranes, which consist of approximately 50% cholesterol
by dry weight. Following infusion, 7% of the infused cholesterol was in the spleen
and significantly increased (by 35%) spleen cholesterol concentration above the
baseline. An acute inflammatory reaction was induced by the subcutaneous injection
of AgNO(3) which also raised spleen cholesterol values, but not significantly. Both
treatments were also administered together and the increase in spleen cholesterol
concentration after 1 h was equivalent to the sum of the individual treatments. In
all the treatment groups, the spleen cholesterol concentration and the plasma SAA
values were then followed over a period of 24 h. In all treatment groups the spleen
cholesterol values fell to baseline values primarily between 18 and 24 h which
coincided with significantly raised levels of plasma SAA. In the case of the dual
treatment, between 4 and 18 h, SAA increased from 92.1 +/- 12.3 to 478 +/- 58.3
microg/ml, respectively and depletion of spleen cholesterol occurred gradually
reaching baseline values after 24 h. The significant flux of cholesterol though the
spleen raises the distinct possibility that the spleen is much more involved in
cholesterol metabolism than previously appreciated. Furthermore, the speed with
which plasma SAA increases following the infusion of fragmented red blood cell
membranes and the role that SAA plays in cholesterol mobilisation raise issues that
may be relevant to alterations in plasma acute phase protein and lipid parameters
in patients undergoing transfusions or suffering from hemolytic disorders.
AN - rayyan-553782242
AU - Li, C.
AU - Kisilevsky, R.
DO - 10.1080/13506120802525210
IS - 4
J2 - Amyloid
KW - Animals
Biological Transport, Active/drug effects
Cholesterol/*metabolism
Erythrocyte Membrane/metabolism
Female
Hemolysis/drug effects/*physiology
Humans
Mice
Models, Biological
Serum Amyloid A Protein/*metabolism
Spleen/drug effects/*metabolism
LA - eng
N1 - Department of Pathology and Molecular Medicine, Queens University, Kingston,
Ontario, Canada.
PY - 2008
SP - 246-54
ST - Serum amyloid A, in vivo splenic cholesterol export and its potential
implications in hemolytic disorders
T2 - Amyloid : the international journal of experimental and clinical
investigation : the official journal of the International Society of Amyloidosis
TI - Serum amyloid A, in vivo splenic cholesterol export and its potential
implications in hemolytic disorders
VL - 15
Y2 - 12
ID - 10152
ER -
TY - JOUR
AB - The two major causes for implant failure are postoperative infection and poor
osteogenesis. Initial period of osteointegration is regulated by immunocytes and
osteogenic-related cells resulting in inflammatory response and tissue healing. The
healing phase can be influenced by various environmental factors and biological
cascade effect. To synthetically orchestrate bone-promoting factors on biomaterial
surface, built is a dual delivery system coated on a titanium surface (abbreviated
as AH-Sr-AgNPs). The results show that this programmed delivery system can release
Ag(+) and Sr(2+) in a temporal-spatial manner to clear pathogens and activate
preosteoblast differentiation partially through manipulating the polarization of
macrophages. Both in vitro and in vivo assays show that AH-Sr-AgNPs-modified
surface renders a microenvironment adverse for bacterial survival and favorable for
macrophage polarization (M2), which further promotes the differentiation of
preosteoblasts. Infected New Zealand rabbit femoral metaphysis defect model is used
to confirm the osteogenic property of AH-Sr-AgNPs implants through micro-CT,
histological, and histomorphometric analyses. These findings demonstrate that the
programmed surface with dual delivery of Sr(2+) and Ag(+) has the potential of
achieving an enhanced osteogenic outcome through favorable immunoregulation.
AN - rayyan-553782226
AU - Li, D.
AU - Li, Y.
AU - Shrestha, A.
AU - Wang, S.
AU - Wu, Q.
AU - Li, L.
AU - Guan, C.
AU - Wang, C.
AU - Fu, T.
AU - Liu, W.
AU - Huang, Y.
AU - Ji, P.
AU - Chen, T.
DO - 10.1002/adhm.201900002
IS - 11
J2 - Adv Healthc Mater
KW - Animals
*Bone and Bones/metabolism/microbiology/pathology
*Coated Materials, Biocompatible/chemistry/pharmacology
Drug Implants/chemistry/pharmacology
Female
Infections/*drug therapy/metabolism/pathology
Mice
Osseointegration/drug effects
Osteogenesis
RAW 264.7 Cells
Rabbits
*Silver/chemistry/pharmacology
*Strontium/chemistry/pharmacology
Surface Properties
*Titanium/chemistry/pharmacology
Program Evaluation
Titanium
Durapatite
Bone and Bones
LA - eng
N1 - Stomatological Hospital of Chongqing Medical University, Chongqing Key
Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key
Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, 401147,
P. R. China.; Stomatological Hospital of Chongqing Medical University, Chongqing
Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key
P. R. China.; Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6,
Canada.; Stomatological Hospital of Chongqing Medical University, Chongqing Key
P. R. China.; Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing, 314001, P.
R. China.; Stomatological Hospital of Chongqing Medical University, Chongqing Key
P. R. China.
PY - 2019
SP - e1900002
ST - Effects of Programmed Local Delivery from a Micro/Nano-Hierarchical Surface
on Titanium Implant on Infection Clearance and Osteogenic Induction in an Infected
Bone Defect
T2 - Advanced healthcare materials
TI - Effects of Programmed Local Delivery from a Micro/Nano-Hierarchical Surface
on Titanium Implant on Infection Clearance and Osteogenic Induction in an Infected
Bone Defect
VL - 8
Y2 - 6
ID - 10136
ER -
TY - JOUR
AB - BACKGROUND: An ideal repairing material characterizes by both great
biocompatibility and osteogenesis ability. Any biomaterials should meet excellent
biological security and biocompatibility prior to clinical application. OBJECTIVE:
To evaluate the biocompatibility and the biological security of the TiO2-Ag-nano-
hydroxyapatite/polyamide66 (TiO 2-Ag-nHA/PA66) composite. DESIGN, TIME AND SETTING:
A randomized controlled duplicated design was performed in Chongqing Medical
University from July 2008 to July 2009. MATERIALS: A total of 40 Kunming mice aged
3 weeks and of clean grade and 32 healthy adult New Zealand rabbits were provided
by Experimental Animal Center of Chongqing Medical University. TiO 2-Ag-nHA/PA66
composite powder (10 g), TiO2-Ag-nHA/PA66 composites (n=32, 5 mm × 25 mm), and
TiO2-Ag-nHA/PA66 composites (n=32, 3 mm × 5 mm) were provided by Research Center of
Nano-Biomaterials of Sichuan University. METHODS: General toxicity test: Forty mice
were randomly divided into experimental and control groups. Composite powder was
used to prepare leaching liquor which was treated on experimental mice by an
intraperitoneal injection. While, an equal amount saline was inserted into the
control mice. Intramuscular implanting test: The 16 rabbits were randomly divided
into experimental and control groups. Two TiO 2-Ag-nHA/PA66 composite (5 mm × 25
mm) were implanted into left and right erector spinae, respectively. A similar
surgery with the exception of implantation was performed on the control rabbits.
Intrabony implanting test: The resting 16 rabbits were implanted one
TiO2-Ag-nHA/PA66 composite (3 mm × 5 mm) into left and right lateral epicondyle,
respectively. Hemolytic test: A 8-mL anti-coagulation blood was obtained and added
with composite powder (0.1, 0.15, and 0.2 g). MAIN OUTCOME MEASURES:
Biocompatibility and biological security of TiO2-Ag-nHA/PA66 composite. RESULTS:
General toxicity test demonstrated that mice in the two groups had good activity
and eating, normal breathing, stable body mass, no paralysis, convulsion, and
death. Intramuscular implanting test and intrabony implanting test showed that
there was no significant difference in levels of alanine aminotransferase,
aspartate transaminase, urea nitrogen, and creatinine and leukocyte numbers between
the two groups before implantation and at day 4, 1 week, and 2 weeks after
implantation (P > 0.05). In particular, there was no significant difference in the
experimental group at varying time points (P > 0.05). Intramuscular implanting test
indicated that tissue sections in the experimental group displayed coated tissue of
materials. The inflammatory variation in the experimental group was generally
similar to that in the control group. Intrabony implanting test indicated that
tissue sections in the experimental group displayed new bone formation. Hemolytic
test suggested that hemolytic ratio of three varying concentrations of TiO2-Ag-
nHA/PA66 composites was less than 5%, which met the normal criterion. CONCLUSION:
TiO2-Ag-nHAPA66 composite has a good biocompatibility and biological security.
AN - rayyan-553780816
AU - Li, G. Z.
AU - Jiang, D. M.
AU - Tan, Z. J.
AU - Lu, M. P.
AU - Kuang, S. R.
AU - Peng, C.
AU - Guo, Z. P.
DO - 10.3969/j.issn.1673-8225.2009.47.005
IS - 47
KW - Animalia
Mus
Oryctolagus cuniculus
polyamide 66
titanium dioxide
titanium dioxide silver nano hydroxyapatite
unclassified drug
aged
article
biocompatibility
breathing
convulsion
death
eating
mouse
nonhuman
rabbit
tissue section
toxicity testing
Durapatite
PY - 2009
SP - 9231-9235
ST - Biocompatibility and biological security of the TiO2-Ag-nano-
hydroxyapatite/polyamide66 composite
T2 - Journal of Clinical Rehabilitative Tissue Engineering Research
TI - Biocompatibility and biological security of the TiO2-Ag-nano-
hydroxyapatite/polyamide66 composite
77953717248&doi=10.3969%2fj.issn.1673-
8225.2009.47.005&partnerID=40&md5=b4d7ccc43266229a1181e7566401f3f5
VL - 13
ID - 8837
ER -
TY - JOUR
AB - Lenalidomide is a potent immunomodulatory agent capable of downregulating
proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) and upregulating
anti-inflammatory cytokines. Lenalidomide has been shown to elicit cardiovascular
effects, although its impact on cardiac function remains obscure. This study was
designed to examine the effect of lenalidomide on cardiac contractile function in
ob/ob obese mice. C57BL lean and ob/ob obese mice were given lenalidomide (50
mg/kg/day, p.o.) for 3 days. Body fat composition was assessed by dual-energy X-ray
absorptiometry. Cardiomyocyte contractile and intracellular Ca(2+) properties were
evaluated. Expression of TNF-α, interleukin-6 (IL-6), Fas, Fas ligand (FasL), the
short-chain fatty acid receptor GPR41, the NFκB regulator IκB, endoplasmic
reticulum (ER) stress, the apoptotic protein markers Bax, Bcl-2, caspase-8, tBid,
cytosolic cytochrome C, and caspase-12; and the stress signaling molecules p38 and
extracellular signal-regulated kinase (ERK) were evaluated by western blot. ob/ob
mice displayed elevated serum TNF-α and IL-6 levels, fat composition and glucose
intolerance, the effects of which except glucose intolerance and fat composition
were attenuated by lenalidomide. Cardiomyocytes from ob/ob mice exhibited depressed
peak shortening (PS) and maximal velocity of shortening/relengthening, prolonged
time-to-PS and time-to-90% relengthening as well as intracellular Ca(2+)
mishandling, which were ablated by lenalidomide. Western blot analysis revealed
elevated levels of TNF-α, IL-6, Fas, Bip, Bax, caspase-8, tBid, cleaved caspase-3
caspase-12, cytochrome C, phosphorylation of p38, and ERK in ob/ob mouse hearts,
the effects of which with the exception of Bip, Bax, and caspase-12 were alleviated
by lenalidomide. Taken together, these data suggest that lenalidomide is protective
against obesity-induced cardiomyopathy possibly through antagonism of cytokine/Fas-
induced activation of stress signaling and apoptosis.
AN - rayyan-553782159
AU - Li, L.
AU - Hua, Y.
AU - Dong, M.
AU - Li, Q.
AU - Smith, D. T.
AU - Yuan, M.
AU - Jones, K. R.
AU - Ren, J.
DO - 10.1038/oby.2012.106
IS - 11
KW - Absorptiometry, Photon
Animals
Blotting, Western
Caspases, Effector/*drug effects
Cells, Cultured
Endoplasmic Reticulum/drug effects
Fas Ligand Protein/drug effects
Lenalidomide
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Myocardial Contraction/*drug effects
Myocytes, Cardiac/*drug effects/metabolism
Obesity/*drug therapy/metabolism/physiopathology
Signal Transduction
Thalidomide/*analogs & derivatives/pharmacology
Tumor Necrosis Factor-alpha/*drug effects
Myocytes, Cardiac
Obesity
LA - eng
N1 - Department of Pharmacology, Xinjiang Medical University, Urumqi, Xinjiang, PR
China.
PY - 2012
SP - 2174-85
ST - Short-term lenalidomide (Revlimid) administration ameliorates cardiomyocyte
contractile dysfunction in ob/ob obese mice
TI - Short-term lenalidomide (Revlimid) administration ameliorates cardiomyocyte
contractile dysfunction in ob/ob obese mice
VL - 20
Y2 - 11
ID - 10070
ER -
TY - JOUR
AB - Background. Silver nanoparticles (AgNPs) have been widely used in many
commercial products due to their excellent antibacterial ability. The AgNPs are
released into the environment, gradually accumulate in the ocean, and may affect
animals at high trophic levels, such as cetaceans and humans, via the food chain.
Hence, the negative health impacts caused by AgNPs in cetaceans are of concern.
Cytokines play a major role in the modulation of immune system and can be
classified into two types: Th1 and Th2. Th1/Th2 balance can be evaluated by the
ratios of their polarizing cytokines (i.e., interferon [IFN]-γ/Interleukin [IL]-4),
and animals with imbalanced Th1/Th2 response may become more susceptible to certain
kinds of infection. Therefore, the present study evaluated the in vitro cytokine
responses of cetacean peripheral blood mononuclear cells (cPBMCs) to 20 nm citrate-
AgNPs (C-AgNP20) by quantitative reverse transcriptase polymerase chain reaction
(qRT-PCR). Methods. Blood samples were collected from six captive common bottlenose
dolphins (Tursiops truncatus). The cPBMCs were isolated and utilized for evaluating
the in vitro cytokine responses. The cytokines evaluated included IL-2, IL-4, IL-
10, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. The geometric
means of two housekeeping genes (HKGs), glyceraldehyde 3-phosphate dehydrogenase
(GAPDH) and β2-microglobulin (B2M), of each sample were determined and used to
Normalize the mRNA expression levels of target genes. Results. The ratio of late
apoptotic/necrotic cells of cPBMCs significantly increased with or without
concanavalin A (ConA) stimulation after 24 h of 10 µg/ml C-AgNP20 treatment. At 4 h
of culture, the mRNA expression level of IL-10 was significantly decreased with 1
µg/ml C-AgNP20 treatment. At 24 h of culture with 1 µg/ml C-AgNP20, the mRNA
expression levels of all cytokines were significantly decreased, with the
exceptions of IL-4 and IL-10. The IFN-γ/IL-4 ratio was significantly decreased at
24 h of culture with 1 µg/ml C-AgNP20 treatment, and the IL-12/IL-4 ratio was
significantly decreased at 4 or 24 h of culture with 0.1 or 1 µg/ml C-AgNP20
treatment, respectively. Furthermore, the mRNA expression level of TNF-α was
significantly decreased by 1 µg/ml C-AgNP20 after 24 h of culture. Discussion. The
present study demonstrated that the sublethal dose of C-AgNP20 (≤1 µg/ml) had an
inhibitory effect on the cytokine mRNA expression levels of cPBMCs with the
evidence of Th2 cytokine bias and significantly decreased the mRNA expression level
of TNF-α. Th2 cytokine bias is associated with enhanced immunity against parasites
but decreased immunity to intracellular microorganisms. TNF-α is a contributing
factor for the inflammatory response against the infection of intracellular
pathogens. In summary, our data indicate that C-AgNP20 suppresses the cellular
immune response and thereby increases the susceptibility of cetaceans to infection
by intracellular microorganisms. © 2018 Li et al.
AN - rayyan-553780827
AU - Li, W. T.
AU - Wang, L. Y.
AU - Chang, H. W.
AU - Yang, W. C.
AU - Lo, C.
AU - Pang, V. F.
AU - Chen, M. H.
AU - Jeng, C. R.
DO - 10.7717/peerj.5432
IS - 9
KW - Cetacean
Cytokine
Immunotoxicity
QRT-PCR
Silver nanoparticles (AgNPs)
Th2 bias
beta 2 microglobulin
gamma interferon
interleukin 10
interleukin 12
interleukin 2
interleukin 4
messenger RNA
silver nanoparticle
animal cell
animal experiment
animal model
animal tissue
apoptosis assay
Article
blood biochemistry
blood cell count
blood sampling
bottlenose dolphin
cell migration assay
controlled study
cytokine response
cytotoxicity
flow cytometry
gene expression
housekeeping gene
immune response
macrophage
nonhuman
oxidative stress
peripheral blood mononuclear cell
pore size distribution
receptor down regulation
T lymphocyte
Th1 Th2 balance
Th2 cell
zeta potential
Cytokines
PY - 2018
ST - Th2 cytokine bias induced by silver nanoparticles in peripheral blood
mononuclear cells of common bottlenose dolphins (Tursiops truncatus)
T2 - PeerJ
TI - Th2 cytokine bias induced by silver nanoparticles in peripheral blood
mononuclear cells of common bottlenose dolphins (Tursiops truncatus)
85053391175&doi=10.7717%2fpeerj.5432&partnerID=40&md5=2f109415887ea750ead34c38efcff
2f4
VL - 2018
ID - 8848
ER -
TY - JOUR
AB - Bacterial infection is one of the most critical obstacles in wound healing,
and severe bacterial infections can lead to inflammatory conditions and delay the
healing process. Herein, a novel hydrogel based on polyvinyl alcohol (PVA), agar,
and silk-AgNPs was prepared using a straightforward one-pot physical cross-linking
method. The in situ synthesis of AgNPs in hydrogels exploited the reducibility of
tyrosine (Tyr tyrosine) in silk fibroin, which endowed the hydrogels with
outstanding antibacterial qualities. In addition, the strong hydrogen bond cross-
linked networks of agar and the crystallites formed by PVA as the physical cross-
linked double network of the hydrogel gave it excellent mechanical stability. The
PVA/agar/SF-AgNPs (PASA) hydrogels exhibited excellent water absorption, porosity,
and significant antibacterial effects against Escherichia coli (E. coli) and
Staphylococcus aureus (S. aureus). Furthermore, in vivo experimental results
confirmed that the PASA hydrogel significantly promoted wound repair and skin
tissue reconstruction by reducing inflammation and promoting collagen deposition.
Immunofluorescence staining showed that the PASA hydrogel enhanced CD31 expression
to promote angiogenesis while decreasing CD68 expression to reduce inflammation.
Overall, the novel PASA hydrogel showed great potential for bacterial infection
wound management. © 2023
AN - rayyan-553780828
AU - Li, W.
AU - Wu, Z.
AU - Zhao, J.
AU - Jiang, M.
AU - Yuan, L.
AU - Guo, Y.
AU - Li, S.
AU - Hu, L.
AU - Xie, X.
AU - Zhang, Y.
AU - Tao, G.
AU - Cai, R.
DO - 10.1016/j.ijbiomac.2023.125652
KW - Antibacterial activity
Dual physically cross-linked hydrogels
Infected wound repair
Mechanical stability
Wound dressing
Algae
Amino acids
Biomechanics
Escherichia coli
Hydrogen bonds
Pathology
Polysaccharides
Polyvinyl alcohols
Repair
agar
CD68 antigen
collagen
hydrogel
polyvinyl alcohol
silk
silk fibroin
silver nanoparticle
tyrosine
Cross-linked hydrogels
Dual physically cross-linked hydrogel
Infected wounds
Inflammatory conditions
Wound dressings
Wound healing
Wound repair
angiogenesis
animal cell
animal experiment
animal model
animal tissue
Article
biocompatibility
compressive strength
controlled study
cross linking
hemolysis
human
human cell
HUVEC cell line
hydrogen bond
hydrophilicity
in vitro study
in vivo study
inflammation
Leporidae
male
mouse
nanofabrication
NCTC clone 929 cell line
nonhuman
one pot synthesis
porosity
protein expression
rat
skin fibroblast
skin injury
stress strain relationship
tensile strength
therapy effect
umbilical vein endothelial cell
water absorption
wound healing
wound infection
Young modulus
Hydrogels
Wound Healing
Polyvinyls
Alcoholics
PY - 2023
ST - Fabrication of dual physically cross-linked polyvinyl alcohol/agar hydrogels
with mechanical stability and antibacterial activity for wound healing
TI - Fabrication of dual physically cross-linked polyvinyl alcohol/agar hydrogels
with mechanical stability and antibacterial activity for wound healing
85165116916&doi=10.1016%2fj.ijbiomac.2023.125652&partnerID=40&md5=f029be98ea901d7c7
77f9d7ed6696039
VL - 247
ID - 8849
ER -
TY - JOUR
AB - The functional activities of gold nanoparticles (AuNPs) on biological systems
depend on their physical-chemical properties and their surface functionalizations.
Within a biological environment and depending on their surface characteristics, NPs
can adsorb biomolecules (mostly proteins) present in the microenvironment, thereby
forming a dynamic biomolecular corona on the surface. The presence of this
biocorona changes the physicalchemical and functional properties of the NPs and how
it interacts with cells. Here, we show that primary human epidermal keratinocytes
(HER) exposed in culture to branched polyethyleneimine (BPEI)-AuNPs, but not to
lipoic acid (LA)-AuNPs, show potent particle uptake, decreased cell viability and
enhanced production of inflammatory factors, while the presence of a human plasma-
derived biocorona decreased NPs uptake and rescued cells from BPEI-AuNP-induced
cell death. The mechanistic study revealed that the intracellular oxidative level
greatly increased after the BPEI-AuNPs treatment, and the transcriptomic analysis
showed that the dominant modulated pathways were related to oxidative stress and an
antioxidant response. The stress level measured by flow cytometry also showed a
significant decrease in the presence of a biocorona. Further anaylsis discovered
that nuclear factor erythroid-2 related factor (Nrf2), a major regulator of anti-
oxidant and anti-inflammatory genes, as the key factor related to the AuNPs induced
oxidative stress and inflammation. This study provides futher understanding into
the mechanisms on how NPs-induced cellular stress and reveals the protective
effects of a biocorona on inflammatory responses in HER at the molecular level,
which provides important insights into the biological responses of AuNPs and their
biocorona.
AN - rayyan-553780829
AU - Li, X. J.
AU - Li, D. J.
AU - Zhang, G. F.
AU - Zeng, Y. Q.
AU - Monteiro-Riviere, N. A.
AU - Chang, Y. Z.
AU - Li, Y.
DO - 10.1016/j.toxlet.2022.08.009
KW - Humanities
Humanism
Humans
Keratinocytes
PY - 2022
SN - 0378-4274 1879-3169
SP - 34-42
ST - Biocorona modulates the inflammatory response induced by gold nanoparticles
in human epidermal keratinocytes
TI - Biocorona modulates the inflammatory response induced by gold nanoparticles
in human epidermal keratinocytes
VL - 369
Y2 - 10 y3 - 1
ID - 8850
ER -
TY - JOUR
AB - Recently gold nanomaterials have been widely applied in the biomedical field,
but their biosafety is still controversial. We immobilized small gold nanoparticles
(AuNPs) on a large silica substrate to form silica-gold core-shell materials
(SiO2@AuNPs) via classical seed-mediated growth. In vitro, 500 nm-SiO2@AuNPs could
promote the proliferation of mouse embryonic fibroblast cells (NIH/3T3). The
results of transmission electron microscope (TEM) showed that the vast majority of
particles did not enter cells and that the morphology of microtubules experienced
no change as observed in the confocal microscope images. The mechanism may be that
the large silica substrate kept AuNPs outside the cells and the nanosize concavo-
convex gold shell facilitated to cell adhesion, resulting in the proliferation. In
vivo, a cutaneous full-thickness excisional wound rat model was applied to assess
the healing efficiency of 500 nm-SiO2@AuNPs. The results indicated that SiO2@AuNPs
could promote wound healing, which was potentially related to the anti-inflammatory
and antioxidation of AuNPs. The pathological finding showed that the healing levels
of SiO2@AuNPs were significantly better than those of the control groups. Our study
may provide insight into the application of silica-gold core-shell materials in the
treatment of cutaneous wounds. (C) 2015 Elsevier Inc. All rights reserved.
AN - rayyan-553780830
AU - Li, X. Q.
AU - Wang, H. F.
AU - Rong, H. L.
AU - Li, W. H.
AU - Luo, Y.
AU - Tian, K.
AU - Quan, D. Q.
AU - Wang, Y. G.
AU - Jiang, L.
DO - 10.1016/j.jcis.2014.12.084
KW - Wound Healing
PY - 2015
SN - 0021-9797 1095-7103
SP - 312-319
ST - Effect of composite SiO2@AuNPs on wound healing: In vitro and vivo studies
T2 - JOURNAL OF COLLOID AND INTERFACE SCIENCE
TI - Effect of composite SiO2@AuNPs on wound healing: In vitro and vivo studies
VL - 445
Y2 - 5 y3 - 1
ID - 8851
ER -
TY - JOUR
AB - The sequence of p38MAPK in silver carp (Hm-p38a) was cloned and sequenced.
Additionally, the acute toxicity of crude microcystins (MCs) on silver carp and
induction expression of Hm-p38a by MCs exposure were also determined in this study.
The results reveal that the length of Hm-p38a is 2418 bp and it contains a 1086 bp
open reading frame. Hm-p38a could encode 361 amino acids. Sequence analysis
indicates that Hm-p38a contains the conserved structures of Thr-Gly-Tyr motif and
substrate binding site Ala-Thr-Arg-Trp, and it is highly conserved in fish.
Phylogenetic analysis reveals that Hm-p38a is more closely related to fish than
mammals and it belongs to p38 alpha subfamily. Moreover, Hm-p38a in silver carp is
constitutively expressed in all examined tissues. In addition, our results indicate
that MCs exposure significantly promotes the transcription of Hm-p38a in fish liver
or kidney, suggesting that mitogen-activated protein kinase should also be the
signal pathway of MCs hepatotoxicity in fish. (C) 2016 Wiley Periodicals, Inc.
AN - rayyan-553780831
AU - Li, X. Y.
AU - Ma, J. G.
AU - Li, Y. Y.
DO - 10.1002/jbt.21781
IS - 5
KW - Kidney
Liver
PY - 2016
SN - 1095-6670 1099-0461
SP - 224-231
ST - Molecular Cloning and Expression Determination of p38 MAPK from the Liver and
Kidney of Silver Carp
T2 - JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
TI - Molecular Cloning and Expression Determination of p38 MAPK from the Liver and
Kidney of Silver Carp
VL - 30
Y2 - 5
ID - 8852
ER -
TY - JOUR
AB - Microcystins produced by some cyanobacteria can cause damages to the liver
and kidneys of aquatic animals. In the natural water with cyanobacterial blooms,
silver carp may suffer from the most serious affect of the bloom due to their
filtering these cyanobacteria and ingesting them as food. In the present study,
silver carp was exposed to microcystin-LR by using the method of intraperitoneal
injection first to determine the acute toxicity of microcystin-LR on silver carp
and then to determine the activity of inflammatory protein and content of
inflammatory factors from the serum of silver carp following a subacute exposure of
microcystin-LR at doses of 104.9 μg kg –1 (1/5 of LD 50 ) or 262.1 μg kg –1 (1/2 of
LD 50 ). The results showed that MC-LR exposure increased fish liver index and
promoted the activities of fish serum alanine aminotransferase (ALT) and aspartate
aminotransferase (AST), indicating the hepatotoxicity of MC-LR on the fish.
Moreover, MC-LR exposure also increased the number of leukocytes, complement C3
level, lysozyme activity (at the first 9 h of exposure), and the contents of
cytokines TNF-α IL-1β and IFN-γ in fish serum. In addition, a significant increase
in IgM level was observed in the serum and head kidney of silver carp following MC-
LR exposure. This result suggests that semi-lethal doses of MC-LR exposure is not
only hepatotoxic but also immunotoxic to silver carp. © 2018 Elsevier Inc.
AN - rayyan-553780832
AU - Li, X.
AU - Li, J.
AU - Meng, F.
AU - Yao, L.
DO - 10.1016/j.ecoenv.2018.10.110
KW - Acute hepatotoxicity
Immunotoxicity
Microcystin-LR
Silver carp
Alanine Transaminase
Animals
Aspartate Aminotransferases
Carps
Cytokines
Head Kidney
Immunoglobulin M
Leukocyte Count
Liver
Microcystins
Animalia
Cyanobacteria
Hypophthalmichthys nobilis
complement component C3
gamma interferon
immunoglobulin M
interleukin 1beta
lysozyme
microcystin LR
autacoid
cytokine
microcystin
cyanobacterium
cyprinid
enzyme activity
immune response
pollution exposure
toxicity
adaptive immunity
animal cell
animal experiment
animal model
animal tissue
Article
carp
complement blood level
controlled study
head kidney
immunoglobulin blood level
immunotoxicity
innate immunity
LD50
leukocyte count
liver toxicity
nonhuman
protein content
silver carp
animal
blood
drug effect
immunology
liver
metabolism
PY - 2019
SP - 28-32
ST - Hepatotoxicity and immunotoxicity of MC-LR on silver carp
TI - Hepatotoxicity and immunotoxicity of MC-LR on silver carp
85056165094&doi=10.1016%2fj.ecoenv.2018.10.110&partnerID=40&md5=04af276cc17e72c9895
459693b18476c
VL - 169
ID - 8853
ER -
TY - JOUR
AB - Recent study suggested that the presence of phytochemicals in food could
interact with nanoparticles (NPs) and consequently reduce the toxicity of NPs,
which has been attributed to the antioxidant properties of phytochemicals. In this
study, we investigated the interactions between ZnO NPs and two flavonoids
baicalein (Ba) or baicalin (Bn) as well as the influence of the interactions on the
toxicity of ZnO NPs to Caco-2 cells. The antioxidant properties of Ba and Bn were
confirmed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-azino-bis(3-
ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, with Ba being stronger.
However, the presence of Ba or Bn did not significantly affect cytotoxicity,
intracellular superoxide or release of inflammatory cytokines of Caco-2 cells after
ZnO NP exposure. When Ba was present, the cellular viability of Caco-2 cells after
exposure to ZnO NPs was slightly increased, associated with a modest decrease of
intracellular Zn ions, but these effects were not statistically different. Ba was
more effective than Bn at changing the hydrodynamic sizes, Zeta potential and UV-
Vis spectra of ZnO NPs, which indicated that Ba might increase the colloidal
stability of NPs. Taken together, the results of the present study indicated that
the anti-oxidative phytochemical Ba might only modestly protected Caco-2 cells from
the exposure to ZnO NPs associated with an insignificant reduction of the
accumulation of intracellular Zn ions. These results also indicated that when
assessing the combined effects of NPs and phytochemicals to cells lining
gastrointestinal tract, it might be necessary to evaluate the changes of colloidal
stability of NPs altered by phytochemicals.
AN - rayyan-553780834
AU - Li, Y. N.
AU - Zhang, C.
AU - Liu, L. L.
AU - Gong, Y.
AU - Xie, Y. X.
AU - Cao, Y.
DO - 10.1080/15376516.2017.1376023
IS - 3
PY - 2018
SN - 1537-6516 1537-6524
SP - 167-176
ST - The effects of baicalein or baicalin on the colloidal stability of ZnO
nanoparticles (NPs) and toxicity of NPs to Caco-2 cells
T2 - TOXICOLOGY MECHANISMS AND METHODS
TI - The effects of baicalein or baicalin on the colloidal stability of ZnO
nanoparticles (NPs) and toxicity of NPs to Caco-2 cells
VL - 28
ID - 8855
ER -
TY - JOUR
AB - The possibility that nanomaterials could perturb the normal course of an
inflammatory response is a key issue when assessing nanoimmunosafety. The
alteration of the normal progress of an inflammatory response may have pathological
consequences, since inflammation is a major defensive mechanism and its efficiency
maintains the body's health. The immunosafety of engineered nanoparticles at
nontoxic concentrations was investigated with the use of a human primary monocyte-
based in vitro system, which reproduces in a simplified fashion the full course of
the physiological inflammatory response, from initiation and development to
resolution. The kinetics of expression and production of inflammatory and anti-
inflammatory cytokines and the proteomic profiles were used for describing the
inflammatory defensive response. We assessed the ability of gold and silver
nanoparticles to trigger inflammation and to interfere with the course of an
ongoing defensive reaction. While neither nanoparticle type was able to directly
activate monocytes, silver nanoparticles could exacerbate the inflammatory response
of monocytes but did not interfere with the resolution of the inflammatory
reaction. These findings support the use of human primary monocyte-based in vitro
assays for realistically investigating the effects of engineered nanopartides on
human innate immune responses, in order to predict the immunological risk of
nanomaterials and implement safe nanoparticle-based applications.
AN - rayyan-553780837
AU - Li, Y.
AU - Italiani, P.
AU - Casals, E.
AU - Valkenborg, D.
AU - Mertens, I.
AU - Baggerman, G.
AU - Nelissen, I.
AU - Puntes, V. F.
AU - Boraschi, D.
DO - 10.1021/acsami.6b06278
IS - 42
KW - Humanities
Humanism
Humans
Monocytes
PY - 2016
SN - 1944-8244 1944-8252
SP - 28437-28447
ST - Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro
Model Based on Human Primary Monocytes
T2 - ACS APPLIED MATERIALS & INTERFACES
TI - Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro
Model Based on Human Primary Monocytes
VL - 8
Y2 - 10 y3 - 26
ID - 8857
ER -
TY - JOUR
AB - Due to the abuse of antibiotics and the emergence of multidrug resistant
microorganisms, medical devices, and related biomaterials are at high risk of
microbial infection during use, placing a heavy burden on patients and healthcare
systems. Metal-phenolic networks (MPNs), an emerging organic-inorganic hybrid
network system developed gradually in recent years, have exhibited excellent
multifunctional properties such as anti-inflammatory, antioxidant, and
antibacterial properties by making use of the coordination between phenolic ligands
and metal ions. Further, MPNs have received widespread attention in antimicrobial
infections due to their facile synthesis process, excellent biocompatibility, and
excellent antimicrobial properties brought about by polyphenols and metal ions. In
this review, different categories of biomaterials based on MPNs (nanoparticles,
coatings, capsules, hydrogels) and their fabrication strategies are summarized, and
recent research advances in their antimicrobial applications in biomedical fields
(e.g., skin repair, bone regeneration, medical devices, etc.) are highlighted.
AN - rayyan-553780838
AU - Li, Y.
AU - Miao, Y.
AU - Yang, L. N.
AU - Zhao, Y. T.
AU - Wu, K. K.
AU - Lu, Z. H.
AU - Hu, Z. Q.
AU - Guo, J. S.
DO - 10.1002/advs.202202684
IS - 27
KW - Phenols
Phenol
Metals
PY - 2022
SN - 2198-3844
ST - Recent Advances in the Development and Antimicrobial Applications of Metal-
Phenolic Networks
T2 - ADVANCED SCIENCE
TI - Recent Advances in the Development and Antimicrobial Applications of Metal-
Phenolic Networks
VL - 9
Y2 - 9
ID - 8858
ER -
TY - JOUR
AB - Implant-associated infections are a major factor contributing to graft
failure. Preventing infection by inhibiting bacterial adhesion to implants is
critical for successful orthopedic surgery. In this work, we fabricated a novel
implant material and evaluated its performance in terms of inhibiting bacterial
growth and adhesion. Micro/nano-structured titanium (MNT) was prepared by the
micro-arc oxidization. Silver nanoparticles (AgNPs) with an average diameter of 10
nm were synthesized via a chemical reduction method and immobilized onto MNT to
generate an AgNP-decorated MNT (AgMN). It showed high antibacterial efficacy
against both Escherichia coli and Staphylococcus aureus without cytotoxicity to
NIH/3T3 fibroblast-like cells. AgMN implanted in rats inhibited S. aureus growth
and adhesion and elicited a milder inflammatory response than MNT. Moreover,
implanted AgMN had no adverse effect on the morphology and structure of attached
host cells after 1 day. These results indicate that using AgMN as an implant
material can reduce the risk of infection without toxic effects to the host.
AN - rayyan-553781845
AU - Li, Y.
AU - Tan, J.
AU - Liu, Z.
AU - Wang, G.
AU - Zhao, C.
AU - Duan, K.
AU - Weng, J.
AU - Feng, B.
DO - 10.1166/jbn.2018.2482
IS - 4
J2 - J Biomed Nanotechnol
KW - Animals
Mice
NIH 3T3 Cells
Rats
Silver
Titanium
LA - eng
PY - 2018
SN - 1550-7033 (Print)
SP - 675-687
ST - Antibacterial Activity and Cyto-/Tissue-Compatibility of Micro-/Nano-
Structured Titanium Decorated with Silver Nanoparticles
T2 - Journal of biomedical nanotechnology
TI - Antibacterial Activity and Cyto-/Tissue-Compatibility of Micro-/Nano-
Structured Titanium Decorated with Silver Nanoparticles
VL - 14
Y2 - 4 y3 - 1
ID - 9771
ER -
TY - JOUR
AB - Due to the increasing applications of nanomaterials and nanotechnology,
potential danger of nanoparticle exposure has become a critical issue. However,
recent nanotoxicity studies have mainly focused on the health risks to healthy
adult population. The nanotoxicity effects on susceptible populations (such as
pregnant, neonate, diseased, and aged populations) have been overlooked. Due to the
alterations in physiological structures and functions in susceptible populations,
they often suffer more damage from the same exposure. Thus, it is urgent to
understand the effects of nanoparticle exposure on these populations. In order to
fill this gap, the potential effects of nanoparticles to pregnant females, neonate,
diseased, and aged population, as well as the possible underlying mechanisms are
reviewed in this article. Investigations show that responses from susceptible
population to nanoparticle exposure are often more severe. Reduced protection
mechanism, compromised immunity, and impaired self-repair ability in these
susceptible populations may contribute to the aggravated toxicity effects. This
review will help minimize adverse effects of nanoparticles to susceptible
population in future nanotechnology applications. © 2014 by the authors; licensee
AN - rayyan-553780841
AU - Li, Y.
AU - Zhang, Y.
AU - Yan, B.
DO - 10.3390/ijms15033671
IS - 3
KW - Aged population
Disease
Health risk
Nanotoxicity
Susceptible populations
Aged
Female
Humans
Infant, Newborn
Nanoparticles
Pregnancy
Pregnancy Complications
Respiration Disorders
Risk Assessment
Risk Factors
cadmium oxide
carbon black nanoparticle
carbon nanotube
chylomicron
gold nanoparticle
immunoglobulin E
immunoglobulin G1
interleukin 10
interleukin 13
interleukin 1beta
interleukin 4
interleukin 5
nanomaterial
nanoparticle
platelet derived growth factor AA
platinum nanoparticle
polystyrene
silver nanoparticle
titanium dioxide
unclassified drug
very low density lipoprotein
apoptosis
asthma
biocompatibility
chronic respiratory tract disease
DNA damage
embryo development
fetus development
fetus malformation
hepatitis
human
immunoreactivity
inflammation
nanotechnology
nanotoxicity
neurotoxicity
nonalcoholic fatty liver
nonhuman
oxidative stress
phagocytosis
placenta disorder
pregnancy
protein degradation
review
thrombosis
toxicity
aged
female
newborn
procedures
risk assessment
risk factor
PY - 2014
SP - 3671-3697
ST - Nanotoxicity overview: Nano-threat to susceptible populations
TI - Nanotoxicity overview: Nano-threat to susceptible populations
84895436939&doi=10.3390%2fijms15033671&partnerID=40&md5=3639ec01ab26287a34f815bd69e
57622
VL - 15
ID - 8860
ER -
TY - JOUR
AB - Diffuse axonal injury (DAI) is one of the most common and severe pathological
consequences of traumatic brain injury (TBI). The molecular mechanism of DAI is
highly complicated and still elusive, yet a clear understanding is crucial for the
diagnosis, treatment, and prognosis of DAI. In our study, we used rats to establish
a DAI model and applied isobaric tags for relative and absolute quantitation
(iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS)
analysis to identify differentially expressed proteins (DEPs) in the corpus
callosum. As a result, a total of 514 proteins showed differential expression
between the injury groups and the control. Among these DEPs, 14 common DEPs were
present at all seven time points postinjury (1, 3, 6, 12, 24, 48, and 72 h). Next,
bioinformatic analysis was performed to elucidate the pathogenesis of DAI, which
was found to possibly involve calcium ion–regulatory proteins (e.g., calsenilin and
ryanodine receptor 2), cytoskeleton organization (e.g., peripherin, NFL, NFM, and
NFH), apoptotic processes (e.g., calsenilin and protein kinase C delta type), and
inflammatory response proteins (e.g., complement C3 and C-reactive protein).
Moreover, peripherin and calsenilin were successfully confirmed by western blotting
to be significantly upregulated during DAI, and immunohistochemical (IHC) analysis
revealed that their expression increased and could be observed in axons after
injury, thus indicating their potential as DAI biomarkers. Our experiments not only
provide insight into the molecular mechanisms of axonal injury in rats during DAI
but also give clinicians and pathologists important reference data for the
diagnosis of DAI. Our findings may expand the list of DAI biomarkers and improve
the postmortem diagnostic rate of DAI. © 2019 Elsevier Inc.
AN - rayyan-553780844
AU - Liang, Y.
AU - Tong, F.
AU - Zhang, L.
AU - Zhu, L.
AU - Li, W.
AU - Huang, W.
AU - Zhao, S.
AU - He, G.
AU - Zhou, Y.
DO - 10.1016/j.brainresbull.2019.09.004
KW - Biomarkers
Diffuse axonal injury
iTRAQ
Proteomics
Rat
Animals
Axons
Brain
Brain Injuries, Traumatic
Chromatography, Liquid
Computational Biology
Corpus Callosum
Diffuse Axonal Injury
Female
Prognosis
Rats
Tandem Mass Spectrometry
biological marker
calcium ion
calpain
calsenilin
peripherin
regulator protein
ribonucleotide
Alzheimer disease
animal experiment
animal model
apoptosis
Article
axon
bioinformatics
biological phenomena and functions concerning the entire organism
brain stem
calcium signaling
cell fractionation
cell organelle
clinician
controlled study
corpus allatum
corpus callosum
cytoskeleton
cytosol
diffuse axonal injury
enzyme activation
extracellular space
hierarchical clustering
Huntington chorea
inflammation
intracellular signaling
mitochondrion
nonhuman
oxidative phosphorylation
Parkinson disease
pathogenesis
pathologist
pathophysiology
priority journal
prognosis
protein degradation
protein expression
protein protein interaction
proteomics
rat
silver staining
swelling
torsion
varicosis
Western blotting
white matter
animal
biology
brain
female
liquid chromatography
metabolism
pathology
procedures
Sprague Dawley rat
tandem mass spectrometry
Proteome
PY - 2019
SP - 289-304
ST - iTRAQ-based proteomic analysis discovers potential biomarkers of diffuse
axonal injury in rats
T2 - Brain Research Bulletin
TI - iTRAQ-based proteomic analysis discovers potential biomarkers of diffuse
85072765169&doi=10.1016%2fj.brainresbull.2019.09.004&partnerID=40&md5=cfc97dc8b62c7
60850f6338e9ee8f400
VL - 153
ID - 8863
ER -
TY - JOUR
AB - Background: The Lonicera japonica has been used as natural and healthy drink
for its anti-inflammatory effect and pleasant odor in China and Taiwan.Methods: 2D
electrophoresis was used to analyze the proteins involved in photoactivated
Lonicera japonica-induced CH27 cell apoptosis. The fluorescent dyes MitoTracker Red
CMXRos, calcein AM and JC-1 were used to elucidate mitochondrial function. The
protein expression was performed by Western blotting. Fluorescent image of
endoplasmic reticulum was accomplished by using ER-Tracker Green. This study used
fluorescent dye CM-H2DCFDA to detect intracellular generation of reactive oxygen
species.Results: The identified proteins can be classified into three major groups,
which include proteins involved in mitochondrial function, cytoskeleton-related
proteins and proteins associated with endoplasmic reticulum (ER) stress.
Photoactivated Lonicera japonica caused a significant effect on the mitochondrial
function and ER stress in CH27 cells. The reactive oxygen species producing was
found to be involved in photoactivated Lonicera japonica-induced CH27 cell
apoptosis.Conclusion: Mitochondria and endoplasmic reticulum are the integral
targets in photoactivated Lonicera japonica-induced CH27 cell apoptosis. We also
demonstrated that ethyl acetate fraction of Lonicera japonica extracts caused
photocytotoxicity in a dose-dependent manner in CH27 cells. This could explain the
fact that the ethyl acetate fraction of Lonicera japonica extracts may contain
compounds which exhibit the photosensitizing activity in CH27 cells. © 2013 Liao et
al.; licensee BioMed Central Ltd.
AN - rayyan-553780845
AU - Liao, J. C.
AU - Chang, W. T.
AU - Lan, Y. H.
AU - Hour, M. J.
AU - Lee, H. Z.
DO - 10.1186/1472-6882-13-244
KW - 2D electrophoresis
Endoplasmic reticulum chaperones
Human lung squamous carcinoma CH27 cells
Lonicera japonica
Mitochondrial chaperones
Photocytotoxicity
Cell Line, Tumor
Cell Survival
Endoplasmic Reticulum Stress
Humans
Lonicera
Lung Neoplasms
Mitochondria
Molecular Chaperones
Oxidative Stress
Photochemical Processes
Plant Extracts
Proteome
Proteomics
calcein
chaperone
fluorescent dye
Lonicera japonica extract
apoptosis
article
controlled study
cytoskeleton
endoplasmic reticulum
endoplasmic reticulum stress
lung squamous cell carcinoma
mitochondrion
nonhuman
nucleotide sequence
photoactivation
photosensitization
phototoxicity
protein analysis
protein expression
proteomics
silver staining
two dimensional gel electrophoresis
Western blotting
Humanities
Humanism
Lung
PY - 2013
ST - Application of proteomics to identify the target molecules involved in
Lonicera japonica-induced photokilling in human lung cancer CH27 cells
T2 - BMC Complementary and Alternative Medicine
TI - Application of proteomics to identify the target molecules involved in
Lonicera japonica-induced photokilling in human lung cancer CH27 cells
84884970889&doi=10.1186%2f1472-6882-13-
244&partnerID=40&md5=e8f408336ceef5b56517da95d937f2bb
VL - 13
ID - 8864
ER -
TY - JOUR
AB - Detection of chemical reactions in living cells is critical in understanding
physiological metabolic processes in the context of nanomedicine. Carbon monoxide
(CO) is one of the important gaseous signaling molecules. Surface-enhanced Raman
spectroscopy (SERS)-based CO-releasing nanoparticles (CORN) is utilized to
investigate the chemical reaction of CO delivery in live cells. Using SERS CORN,
carbonyl dissociation from CORN-Ag-CpW(CO)3to CORN-Ag-CpW(CO)2in live cells is
observed. The subsequent irreversible degradation to CO-free CORN is a consequence
of oxidative stress in cells. This observation affirms the step transition of CORN-
Ag-CpW(CO)3in cellular: CORN-Ag-CpW(CO)3first proceedsviaa direct loss of one CO
followed by a oxidative decomposition giving rise to CORN-Ag-WO3and as well as the
release of one equivalents of CO. Importantly, the decarbonylation process can be
correlated with the level of inflammatory biomarkers. For the first time, we
provide unambiguous evidence for the steps transition of CO-release mechanism in
cellular. © The Royal Society of Chemistry 2020.
AN - rayyan-553780846
AU - Liao, P. H.
AU - Tseng, C. Y.
AU - Ke, Z. Y.
AU - Hsieh, C. L.
AU - Kong, K. V.
DO - 10.1039/d0cc01297a
IS - 35
KW - Animals
Carbon Monoxide
Cell Line
Cytokines
Humans
Metal Nanoparticles
Mice
Silver
Spectrum Analysis, Raman
Tungsten
Carbon monoxide
Cells
Chemical detection
Degradation
Raman spectroscopy
carbon monoxide
carbon monoxide releasing nanoparticle
carbonyl derivative
doxorubicin
interleukin 1beta
interleukin 6
nanoparticle
silver
tungsten derivative
tungsten tricarbonyl complex
unclassified drug
cytokine
metal nanoparticle
tungsten
Detection of chemicals
Irreversible degradation
Metabolic process
Oxidative decomposition
Release mechanism
Signaling molecules
Step transitions
Surface enhanced Raman spectroscopy
animal cell
Article
cell viability
chemical bond
chemical reaction
controlled study
cytokine release
cytotoxicity
decarbonylation
dissociation
fluorescence imaging
human
human cell
mouse
nonhuman
oxidation
oxidative decomposition
oxidative stress
Raman spectrometry
RAW 264.7 cell line
skin fibroblast
animal
cell line
metabolism
Cytology
PY - 2020
SP - 4852-4855
ST - Operando characterization of chemical reactions in single living cells using
SERS
T2 - Chemical Communications
TI - Operando characterization of chemical reactions in single living cells using
SERS
85084192850&doi=10.1039%2fd0cc01297a&partnerID=40&md5=406183ea810a7ee0246ee2b292409
c62
VL - 56
ID - 8865
ER -
TY - JOUR
AB - OBJECTIVE: This study aimed to investigate the effects of liraglutide on
the body weight set point (BWSP) in diet-induced obese rats and to determine the
relationship between BWSP and hypothalamic arcuate nucleus (ARC) microglial
activation. METHODS: Diet-induced obesity (DIO) rats were divided into three
groups: continuous high-fat diet (HFD) plus saline, HFD with liraglutide, and HFD
with liraglutide pair feeding. Body weight, BWSP, inflammatory cytokines,
suppressor of cytokine signaling 3, orexigenic/anorexigenic proteins, apoptosis,
and microglia in the ARC were assessed. The effect of liraglutide on the Notch-1
signaling pathway and its relationships with nuclear factor-κB and p38 mitogen-
activated protein kinase were also investigated in a lipopolysaccharide (LPS)-
induced microglia activation model. RESULTS: Liraglutide reduced BWSP; reversed
adverse changes in hypothalamic inflammation, suppressor of cytokine signaling 3,
and apoptosis; and diminished microgliosis in DIO rats. The BWSP showed a linear
correlation with ARC microglial density. Liraglutide inhibited LPS-induced M1
microglial polarization and promoted microglial polarization to the M2 phenotype,
diminishing inflammatory cytokine expression. Liraglutide inhibited Notch-1
signaling pathway activation and decreased nuclear factor-κB and p38 mitogen-
activated protein kinase pathway activation in LPS-stimulated microglia.
CONCLUSIONS: Liraglutide can reduce BWSP in DIO rats. There is a linear correlation
between hypothalamic microgliosis and BWSP. Liraglutide reduces excessive
microglial activation and inflammation, which may contribute to BWSP reduction.
AN - rayyan-553782385
AU - Liao, T.
AU - Zhang, S. L.
AU - Yuan, X.
AU - Mo, W. Q.
AU - Wei, F.
AU - Zhao, S. N.
AU - Yang, W.
AU - Liu, H.
AU - Rong, X.
DO - 10.1002/oby.22666
IS - 1
KW - Animals
Diet, High-Fat/*adverse effects
Humans
Hypoglycemic Agents/pharmacology/*therapeutic use
Hypothalamus/*drug effects/pathology
Liraglutide/pharmacology/*therapeutic use
Male
Microglia/*drug effects
Obesity/*drug therapy
Rats
Body Weight
Microglia
LA - eng
N1 - Department of Geriatric Endocrinology, The First Affiliated Hospital of
Guangxi Medical University, Nanning, China.; Department of Geriatric Endocrinology,
The First Affiliated Hospital of Guangxi Medical University, Nanning, China.;
Department of Geriatric Endocrinology, The First Affiliated Hospital of Guangxi
Medical University, Nanning, China.; Department of Geriatric Endocrinology, The
First Affiliated Hospital of Guangxi Medical University, Nanning, China.;
Medical University, Nanning, China.
PY - 2020
SP - 122-131
ST - Liraglutide Lowers Body Weight Set Point in DIO Rats and its Relationship
with Hypothalamic Microglia Activation
TI - Liraglutide Lowers Body Weight Set Point in DIO Rats and its Relationship
with Hypothalamic Microglia Activation
VL - 28
Y2 - 1
ID - 10292
ER -
TY - JOUR
AB - Urinary tract infections accounts for over 40% of all nosocomial infections,
and almost all these infections are associated with indwelling catheters. The
acquisition of urinary tract infections following urinary bladder catheterizations
are associated with nearly a threefold increase in mortality among hospitalized
patients. The economic impact of nosocomial urinary infections is difficult to
assess. An estimate of the cost of these infections have shown that patients with
hospital-acquired urinary tract infections secondary to indwelling catheters, spent
an average of 2.4 additional days in the hospital. Bearing this in mind, even a
marginal decrease in urinary tract infections may be cost-effective. In two
randomized prospective clinical studies we have shown that coating urinary
catheters with silver significantly reduces the infection rate during short-term
catheterization (less than 7 days). We also showed that silver coating of urinary
catheters prevented adherence and growth of Pseudomonas aeruginosa on the catheter
material. Another risk from using indwelling catheters is an inflammatory reaction
of the urethral mucosa leading to stricture formation. Several aetiological factors
whereby indwelling catheters may cause a urethral stricture have been discussed.
During the last years much attention has been paid to the catheter material as
such, especially latex, and its role in stricture formation. Urinary catheters are
made from a variety of materials combined with different chemicals. It seems as if
these chemical substances can dissolve from the catheter material, causing
inflammatory reactions. Using a cell culture technique with a mouse fibroblast cell
line (BALBc/3T3), and an animal model with implantation of catheter material into
the urethra, we assessed both in vitro cytotoxicity (IC50) and inflammatory
reactions in vivo from different catheter materials. The studies confirmed that
especially latex materials do not have both cytotoxic effects and cause
considerable inflammation within the urethral mucosa. By coating the catheters with
silver, the cytotoxicity could be significantly reduced as compared with pure latex
and hydrogel coated latex catheters. Several studies have demonstrated a cytotoxic
effect from catheter materials, indicating that this may be of importance in
urethral inflammation. However, the exact mechanisms behind this phenomenon is not
known. In an attempt to explain the inflammatory reaction within the urethra
secondary to an indwelling catheter, we investigated the influence of the nervous
system on experimentally induced urethral inflammation. Our findings indicate that
an important part in catheter induced inflammation is played by neurogenic
reactions.
AN - rayyan-553780847
AU - Liedberg, H.
KW - Animals
Bacterial Adhesion
Cross Infection
Female
Humans
Hydrogel
Latex
Polytetrafluoroethylene
Rats
Silicones
Silver
Urethritis
Urinary Catheterization
Urinary Tract Infections
latex
macrogol derivative
politef
silicone derivative
silver
animal
article
bacterium adherence
catheterization
cross infection
female
human
hydrogel
instrumentation
rat
rat strain
urethritis
Urinary Tract
PY - 1989
SP - 1-43
ST - Catheter induced urethral inflammatory reaction and urinary tract infection.
An experimental and clinical study
T2 - Scandinavian journal of urology and nephrology. Supplementum
TI - Catheter induced urethral inflammatory reaction and urinary tract infection.
An experimental and clinical study
0024809594&partnerID=40&md5=6c6caeadfb5a98801a649e3a8f458ad0
VL - 124
ID - 8866
ER -
TY - JOUR
AB - Because of the limited information on size-dependent particle-mediated
effects, the present study was conducted to determine if the changes in induced
protein expression between 5 nm silver nanoparticles and 100 nm particles after
exposure to sub-lethal concentrations. A total of 28,000 cDNA profiles were
screened using 5 nm silver nanoparticles and 100 nm silver nanoparticles in a
macrophage cell line. Based on results obtained from cDNA microarray we also
assessed protein levels of hemeoxygenase-1 (HO-1), heat shock protein-70 (HSP-70)
and interleukin-8 (IL-8), which were shown to significantly increase. Together with
results obtained using N-acetylcystein (NAC), we were able to clearly show that low
level and early stage exposure to 5 nm silver nanoparticles, but not 100 nm,
induces expression of IL-8 as well as stress genes against reactive oxygen species
(ROS). Therefore, we provide important data to understand and identify the early
effects of silver nanoparticles on the immune system. (C) 2012 Elsevier Ltd. All
rights reserved.
AN - rayyan-553780848
AU - Lim, D. H.
AU - Jang, J.
AU - Kim, S.
AU - Kang, T.
AU - Lee, K.
AU - Choi, I. H.
IS - 18
KW - Humanities
Humanism
Humans
Macrophages
PY - 2012
SN - 0142-9612 1878-5905
SP - 4690-4699
ST - The effects of sub-lethal concentrations of silver nanoparticles on
inflammatory and stress genes in human macrophages using cDNA microarray analysis
T2 - BIOMATERIALS
TI - The effects of sub-lethal concentrations of silver nanoparticles on
inflammatory and stress genes in human macrophages using cDNA microarray analysis
VL - 33
Y2 - 6
ID - 8867
ER -
TY - JOUR
AB - Inflenza A viruses (IAVs) are highly transmissible and pathogenic
Orthomyxoviruses, which have led to worldwide outbreaks and seasonal pandemics of
acute respiratory diseases, causing serious threats to public health. Currently
used anti-influenza drugs may cause neurological side effects, and they are
increasingly less effective against mutant strains. To help prevent the spread of
IAVs, in this work, we have developed quercetin-derived carbonized nanogels
(CNGsQur) that display potent viral inhibitory, antioxidative, and anti-
inflammatory activities. The antiviral CNGsQur were synthesized by mild
carbonization of quercetin (Qur), which successfully preserved their antioxidative
and anti-inflammatory properties while also contributed enhanced properties, such
as water solubility, viral binding, and biocompatibility. Antiviral assays of co-
treatment, pre-treatment, and post-treatment indicate that CNGsQur interacts with
the virion, revealing that the major antiviral mechanism resulting in the
inhibition of the virus is by their attachment on the cell surface. Among them, the
selectivity index (SI) of CNGsQur270 (>857.1) clearly indicated its great potential
for clinical application in IAVs inhibition, which was much higher than that of
pristine quercetin (63.7) and other clinical drugs (4-81). Compared with quercetin
at the same dose, the combined effects of viral inhibition, antioxidative and anti-
inflammatory activities impart the superior therapeutic effects of CNGsQur270
aerosol inhalation in the treatment of IAVs infection, as evidenced by a mouse
model. These CNGsQur effectively prevent the spread of IAVs and suppress virus -
induced inflammation while also exhibiting good in vivo biocompatibility. CNGsQur
shows much promise as a clinical therapeutic agent against infection by IVAs. (c)
2022 Elsevier Inc. All rights reserved.
AN - rayyan-553780853
AU - Lin, H. Y.
AU - Zeng, Y. T.
AU - Lin, C. J.
AU - Harroun, S. G.
AU - Anand, A.
AU - Chang, L.
AU - Wu, C. J.
AU - Lin, H. J.
AU - Huang, C. C.
DO - 10.1016/j.jcis.2022.04.124
KW - Orthomyxoviridae
Influenza, Human
Antiviral Agents
PY - 2022
SN - 0021-9797 1095-7103
SP - 481-493
ST - Partial carbonization of quercetin boosts the antiviral activity against H1N1
influenza A virus
T2 - JOURNAL OF COLLOID AND INTERFACE SCIENCE
TI - Partial carbonization of quercetin boosts the antiviral activity against H1N1
influenza A virus
VL - 622
Y2 - 9 y3 - 15
ID - 8872
ER -
TY - JOUR
AB - Background: Alzheimer disease (AD) is the most common type of dementia which
is becoming a primary problem in the present society, but it lacks effective
treatment methods and means of AD. Tanshinone IIA (Tan IIA) has been reported to
have neuroprotective effects to restrain the Aβ25-35-mediated apoptosis. However,
few studies try to understand how Aβ1-42 affects hyperphosphorylation of tau and
how Tan IIA regulates this process at the molecular level. Methods: Fifty male
Sprague-Dawley rats were randomly divided into 5 groups and infused through the
lateral ventricle with Aβ1-42 except the control group. Then the rats were treated
with Tan IIA through intragastric administration for 4 weeks. After the ability of
learning and memory being measured, histomorphological examination and Western blot
were used to detect the possible mechanism in the AD-associated model rats.
Results: We observed that Aβ1-42 infusion could induce spatial learning and memory
deficits in rats. Simultaneously, Aβ1-42 also could reduce the neuron in cornu
ammonis 1 and dentate gyrus of hippocampus, as well as increase the levels of
cleaved caspase 3, hyperphosphorylated tau at the sites Ser396, Ser404, and Thr205
with enhancing staining of black granules in brain. We also found that Aβ1-42 could
increase the activity of extracellular signal-regulated protein kinase (ERK) and
glycogen synthase kinase-3β (GSK-3β). Meanwhile, these phenomena could be
ameliorated when Tan IIA was used. Conclusion: We concluded that Tan IIA might have
neuroprotective effect and improving learning and memory ability to be a viable
candidate in AD therapy with mechanisms involving the ERK and GSK-3β signal
pathway. © The Author(s) 2019.
AN - rayyan-553780858
AU - Lin, L.
AU - Jadoon, S. S.
AU - Liu, S. Z.
AU - Zhang, R. Y.
AU - Li, F.
AU - Zhang, M. Y.
AU - Ai-Hua, T.
AU - You, Q. Y.
AU - Wang, P.
DO - 10.1177/0891988719837373
IS - 3
KW - Alzheimer disease
extracellular signal-regulated protein kinase
glycogen synthase kinase-3β
hyperphosphorylated tau protein
spatial learning and memory deficits
tanshinones IIA
Abietanes
Animals
Glycogen Synthase Kinase 3 beta
Humans
Male
MAP Kinase Signaling System
Memory Disorders
Rats
Spatial Learning
amyloid beta protein[1-42]
caspase 3
glycogen synthase kinase 3beta
tanshinone IIA
tau protein
tanshinone
amnesia
animal experiment
animal model
animal tissue
Article
brain slice
cell loss
cognitive defect
controlled study
drug mechanism
hippocampal CA1 region
hippocampus
histology
male
MAPK signaling
memory consolidation
Morris water maze test
nerve degeneration
nerve fiber
neuroapoptosis
Nissl staining
nonhuman
phosphorylation
priority journal
protein expression
rat
silver staining
spatial learning
Western blotting
animal
disease model
drug effect
human
memory disorder
pharmacology
Sprague Dawley rat
Memory
PY - 2019
SP - 152-163
ST - Tanshinone IIA Ameliorates Spatial Learning and Memory Deficits by Inhibiting
the Activity of ERK and GSK-3β
T2 - Journal of Geriatric Psychiatry and Neurology
TI - Tanshinone IIA Ameliorates Spatial Learning and Memory Deficits by Inhibiting
the Activity of ERK and GSK-3β
85063339362&doi=10.1177%2f0891988719837373&partnerID=40&md5=c00f834bd68c9e57818339f
ff502ad0c
VL - 32
ID - 8877
ER -
TY - JOUR
AB - The employment of nanoparticles has markedly increased in recent years for
different applications such as aerospace technology, electronics, biology, and
medicine. The exposure of nanoparticles to humans is inevitable nowadays.
Neutrophils act as the predominant phagocytic cells for first-line defense to be
recruited to an inflammatory site against xenobiotics. It is important to explore
how neutrophils interact with nanoparticles to elicit immune responses. Different
types of nanoparticles have been studied to reveal a potential interaction to
neutrophils in vitro and in vivo. These mainly include metallic nanoparticles,
polymeric nanoparticles, silica nanoparticles, lipid nanoparticles, and fullerenes.
A number of investigations have reported the toxicity of nanoparticles induced by
neutrophil activation. In this review we discuss data demonstrating recently
recognized aspects of inflammation induced by overwhelmed neutrophils after
nanoparticle treatment. Besides the dark side of the nanoparticles, some
therapeutic nanoparticles are developed and beneficial in treating neutrophil-
related diseases such as acute lung injury, vascular inflammation, and bacterial
infection. Some nanoparticles can recruit neutrophils around tumor sites for
immunotherapy. We also summarize how nanoparticles actively target neutrophils with
therapeutic aims. This review provides a broad introduction to nanoparticle
interplay with neutrophils and discusses in vitro and in vivo studies in which they
have been evaluated.
AN - rayyan-553780859
AU - Lin, M. H.
AU - Lin, C. F.
AU - Yang, S. C.
AU - Hung, C. F.
AU - Fang, J. Y.
DO - 10.1166/jbn.2018.2459
IS - 1
PY - 2018
SN - 1550-7033 1550-7041
SP - 66-85
ST - The Interplay Between Nanoparticles and Neutrophils
TI - The Interplay Between Nanoparticles and Neutrophils
VL - 14
Y2 - 1
ID - 8878
ER -
TY - JOUR
AB - The bacterial exopolysaccharide Curdlan has a unique collagen-like triple
helical structure and immune-modulation activities. Although there have been
several types of Curdlan gels reported for antibacterial or wound healing purposes,
none of them exhibit favorable mechanical properties for clinically applicable
wound healing materials. Herein, we present a two-step approach for preparing Ag-
embedded Curdlan hydrogels that are highly soft but are very stretchable compared
with common polysaccharide-based hydrogels. Ag ions were first reduced in a diluted
Curdlan solution to form AgNP-decorated triple helices. Then, the aqueous solution
consisting of Curdlan/Ag nanoparticles was mixed with a dimethyl sulfoxide solution
consisting of a high concentration of Curdlan. This mixing triggered the
conformation transformation of Curdlan random coils into triple helices, and then
the helices were further packed into semicrystalline nanofibrils of ∼20 nm in
diameter. Due to the presence of semicrystalline fibrils, this novel Curdlan
hydrogel exhibits a fracture strain of ∼350% and fracture stress of ∼0.2 MPa at a
water content of ∼97%. This nanofibril hydrogel supported the attachment,
spreading, and growth of fibroblasts and effectively inhibited the growth of Gram-
negative Escherichia coli and Gram-positive Staphylococcus aureus. Moreover, the
hydrogels downregulated NO production and proinflammatory gene expression levels in
lipopolysaccharide (LPS)-stimulated macrophages but did not change the anti-
inflammatory gene expression levels in IL-4-stimulated macrophages. In an animal
study, these hydrogels accelerated wound healing in a bacteria-infected mice skin
wound model. These results validate the further development of Curdlan/AgNPs
nanofibril hydrogels in clinical wound management.
AN - rayyan-553781808
AU - Lin, M.
AU - Long, H.
AU - Liang, M.
AU - Chu, B.
AU - Ren, Z.
AU - Zhou, P.
AU - Wu, C.
AU - Liu, Z.
AU - Wang, Y.
DO - 10.1021/acsami.1c06603
IS - 31
J2 - ACS Appl Mater Interfaces
KW - Animals
Anti-Bacterial Agents/chemistry/*therapeutic use/toxicity
Anti-Inflammatory Agents/chemistry/*therapeutic use/toxicity
Carbohydrate Conformation/drug effects
Hydrogels/chemistry/*therapeutic use/toxicity
Male
Metal Nanoparticles/chemistry/therapeutic use/toxicity
Mice
Mice, Inbred BALB C
NIH 3T3 Cells
Nanofibers/chemistry/*therapeutic use/toxicity
Silver/chemistry/*therapeutic use/toxicity
Skin/pathology
Staphylococcal Skin Infections/drug therapy/pathology
Tensile Strength
Wound Healing/drug effects
beta-Glucans/chemistry/*therapeutic use/toxicity
LA - eng
N1 - Guangdong Provincial Key Laboratory of Bioengineering Medicine, National
Engineering Research Center of Genetic Medicine, Institute of Biomedicine, College
of Life Science and Technology, Jinan University, Guangzhou 510632, People's
Republic of China.; Guangdong Provincial Key Laboratory of Bioengineering Medicine,
National Engineering Research Center of Genetic Medicine, Institute of Biomedicine,
College of Life Science and Technology, Jinan University, Guangzhou 510632,
People's Republic of China.; Guangdong Provincial Key Laboratory of Bioengineering
Medicine, National Engineering Research Center of Genetic Medicine, Institute of
Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou
510632, People's Republic of China.; Key Laboratory of Biomedical Materials and
Implant Devices, Research Institute of Tsinghua University in Shenzhen, Shenzhen
518057, P.R. China.; Guangdong Provincial Key Laboratory of Bioengineering
Medicine, National Engineering Research Center of Genetic Medicine, Institute of
Biomedicine, College of Life Science and Technology, Jinan University, Guangzhou
510632, People's Republic of China.; Guangdong Provincial Key Laboratory of
Bioengineering Medicine, National Engineering Research Center of Genetic Medicine,
Institute of Biomedicine, College of Life Science and Technology, Jinan University,
Guangzhou 510632, People's Republic of China.; Guangdong Provincial Key Laboratory
of Bioengineering Medicine, National Engineering Research Center of Genetic
Medicine, Institute of Biomedicine, College of Life Science and Technology, Jinan
University, Guangzhou 510632, People's Republic of China.; Guangdong Provincial Key
Laboratory of Bioengineering Medicine, National Engineering Research Center of
Genetic Medicine, Institute of Biomedicine, College of Life Science and Technology,
Jinan University, Guangzhou 510632, People's Republic of China.; Guangdong
Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research
Center of Genetic Medicine, Institute of Biomedicine, College of Life Science and
Technology, Jinan University, Guangzhou 510632, People's Republic of China.
PY - 2021
SP - 36747-36756
ST - Antifracture, Antibacterial, and Anti-inflammatory Hydrogels Consisting of
Silver-Embedded Curdlan Nanofibrils
T2 - ACS applied materials & interfaces
TI - Antifracture, Antibacterial, and Anti-inflammatory Hydrogels Consisting of
Silver-Embedded Curdlan Nanofibrils
VL - 13
Y2 - 8 y3 - 11
ID - 9738
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) are well known to interact with cells, leading to
different cell behaviors such as cell proliferation and differentiation capacity.
Biocompatibility and biological functions enhanced by nanomedicine are the most
concerning factors in clinical approaches. In the present research, AuNP solutions
were prepared at concentrations of 1.25, 2.5, 5 and 10 ppm for biocompatibility
investigations. Ultraviolet-visible spectroscopy was applied to identify the
presence of AuNPs under the various concentrations. Dynamic Light Scattering assay
was used for the characterization of the size of the AuNPs. The shape of the AuNPs
was observed through a Scanning Electron Microscope. Afterward, the mesenchymal
stem cells (MSCs) were treated with a differentiation concentration of AuNP
solutions in order to measure the biocompatibility of the nanoparticles. Our
results demonstrate that AuNPs at 1.25 and 2.5 ppm could significantly enhance MSC
proliferation, decrease reactive oxygen species (ROS) generation and attenuate
platelet/monocyte activation. Furthermore, the MSC morphology was observed in the
presence of filopodia and lamellipodia while being incubated with 1.25 and 2.5 ppm
AuNPs, indicating that the adhesion ability was enhanced by the nanoparticles. The
expression of matrix metalloproteinase (MMP-2/9) in MSCs was found to be more
highly expressed under 1.25 and 2.5 ppm AuNP treatment, relating to better cell
migrating ability. Additionally, the cell apoptosis of MSCs investigated with
Annexin-V/PI double staining assay and the Fluorescence Activated Cell Sorting
(FACS) method demonstrated the lower population of apoptotic cells in 1.25 and 2.5
ppm AuNP treatments, as compared to high concentrations of AuNPs. Additionally,
results from a Western blotting assay explored the possibility that the anti-
apoptotic proteins Cyclin-D1 and Bcl-2 were remarkably expressed. Meanwhile, real-
time PCR analysis demonstrated that the 1.25 and 2.5 ppm AuNP solutions induced a
lower expression of inflammatory cytokines (TNF-alpha, IL-1 beta, IFN-gamma, IL-6
and IL-8). According to the tests performed on an animal model, AuNP 1.25 and 2.5
ppm treatments exhibited the better biocompatibility performance, including anti-
inflammation and endothelialization. In brief, 1.25 and 2.5 ppm of AuNP solution
was verified to strengthen the biological functions of MSCs, and thus suggests that
AuNPs become the biocompatibility nanomedicine for regeneration research.
AN - rayyan-553780861
AU - Lin, R. H.
AU - Lee, H. T.
AU - Yeh, C. A.
AU - Yang, Y. C.
AU - Shen, C. C.
AU - Chang, K. B.
AU - Liu, B. S.
AU - Hsieh, H. H.
AU - Wang, H. M. D.
AU - Hung, H. S.
DO - 10.3390/ijms24010005
IS - 1
PY - 2023
SN - 1422-0067
ST - Favorable Biological Performance Regarding the Interaction between Gold
Nanoparticles and Mesenchymal Stem Cells
TI - Favorable Biological Performance Regarding the Interaction between Gold
Nanoparticles and Mesenchymal Stem Cells
VL - 24
Y2 - 1
ID - 8879
ER -
TY - JOUR
AB - Objective. Currently, infections due to foreign-body reactions caused by
bacteria or implant materials at the wound site are one of the major reasons for
the failure of guided tissue regeneration (GTR) and guided bone regeneration (GBR)
in clinical applications. The purpose of this study was to develop regeneration
membranes with localized cobalt ion release to reduce infection and inflammation by
polycaprolactone (PCL)/cobalt-substituted hydroxyapatite (CoHA). Methods. The PCL
composite membrane containing 20 wt% CoHA powders was prepared by solvent casting.
The surface morphology, crystal structure, chemical composition and thermal
properties of PCL composite membranes were characterized. The biocompatibility,
osteogenic differentiation and antibacterial properties of composite membrane were
also investigated. Then, in biodegradability was assessed by immersing phosphate
buffer solution (PBS) for 6 months. Results. Physicochemical analyses revealed that
CoHA is evenly mixed in the membranes and assistance reduce the crystallinity of
PCL for getting more degradation amounts than PCL membrane. Osteoblast cells
culture on the membrane showed that the CoHA significantly increases cell
proliferation and found the calcium deposition production increased over 90%
compared with PCL after 7 days of culture. A good antibacterial effect was achieved
by the addition of CoHA powder. The results were confirmed by 2.4 times reduction
of proliferation of Escherichia coli (E. coli) seeded on the composite membrane
after 24 h. Immersing in PBS for 6 months indicated that PCL-CoHA composite
membrane has improved biodegradation and can continuously remove free radicals to
reduce the inflammatory response. Significance. The PCL-CoHA composite membrane
with suitable releasing of cobalt ion can be considered as a potential choice for
bone tissue regeneration. (C) 2019 The Academy of Dental Materials. Published by
Elsevier Inc. All rights reserved.
AN - rayyan-553780862
AU - Lin, W. C.
AU - Yao, C. M.
AU - Huang, T. Y.
AU - Cheng, S. J.
AU - Tang, C. M.
DO - 10.1016/j.dental.2019.02.023
IS - 5
KW - Durapatite
PY - 2019
SN - 0109-5641 1879-0097
SP - 751-762
ST - Long-term in vitro degradation behavior and biocompatibility of
polycaprolactone/cobalt-substituted hydroxyapatite composite for bone tissue
engineering
T2 - DENTAL MATERIALS
TI - Long-term in vitro degradation behavior and biocompatibility of
polycaprolactone/cobalt-substituted hydroxyapatite composite for bone tissue
engineering
VL - 35
Y2 - 5
ID - 8880
ER -
TY - JOUR
AB - Diabetic mellitus is one of the leading causes of chronic wounds and remains
a challenging issue to be resolved. Herein, a hydrogel with conformal tissue
adhesivity, skin-like conductivity, robust mechanical characteristics, as well as
active antibacterial function is developed. In this hydrogel, silver nanoparticles
decorated polypyrrole nanotubes (AgPPy) and cobalt ions (Co2+) are introduced into
an in situ polymerized poly(acrylic acid) (PAA) and branched poly(ethylenimine)
(PEI) network (PPCA hydrogel). The PPCA hydrogel provides active antibacterial
function through synergic effects from protonated PEI and AgPPy nanotubes, with a
tissue-like mechanical property (approximate to 16.8 +/- 4.5 kPa) and skin-like
electrical conductivity (approximate to 0.048 S m(-1)). The tensile and shear
adhesive strength (approximate to 15.88 and approximate to 12.76 kPa, respectively)
of the PPCA hydrogel is about two- to threefold better than that of fibrin glue. In
vitro studies show the PPCA hydrogel is highly effective against both gram-positive
and gram-negative bacteria. In vivo results demonstrate that the PPCA hydrogel
promotes diabetic wounds with accelerated healing, with notable inflammatory
reduction and prominent angiogenesis regeneration. These results suggest the PPCA
hydrogel provide a promising approach to promote diabetic wound healing.
AN - rayyan-553780863
AU - Lin, Z. C.
AU - Fan, D. H.
AU - Li, G. J.
AU - He, L. M.
AU - Qin, X. Y.
AU - Zhao, B.
AU - Wang, Q.
AU - Liang, W. L.
DO - 10.1002/mabi.202200349
IS - 2
KW - Wound Healing
PY - 2023
SN - 1616-5187 1616-5195
ST - Antibacterial, Adhesive, and Conductive Hydrogel for Diabetic Wound Healing
T2 - MACROMOLECULAR BIOSCIENCE
TI - Antibacterial, Adhesive, and Conductive Hydrogel for Diabetic Wound Healing
VL - 23
Y2 - 2
ID - 8881
ER -
TY - JOUR
AB - The concentration and distribution Of trace and major elements in cells are
of great interest in cell biology. PIXE can provide elemental concentrations in the
bulk of cells or organelles as other bulk techniques such as atomic absorption
spectrophotometry and nuclear activation analysis. Supplementary information,
perhaps more exciting, on the intracellular distributions of trace elements can be
provided using nuclear microscopy. Intracellular distributions of trace elements in
normal and malignant cells are presented. The toxicity of mercury and cadmium can
be prevented by supplementation of the essential trace element selenium. Some
results from an experimental animal model are discussed. The intercellular
distribution of major and trace elements in isolated blood cells, as revealed by
nuclear microscopy, provides useful clinical information. Examples are given
concerning inflammatory connective-tissue diseases and the chronic fatigue
syndrome.
AN - rayyan-553780864
AU - Lindh, U.
DO - 10.1016/0168-583X(93)95553-H
IS - 1
KW - Trace Elements
Nuclear Microscopy
PY - 1993
SN - 0168-583X 1872-9584
SP - 261-267
ST - NUCLEAR MICROSCOPY IN TRACE-ELEMENT BIOLOGY - FROM CELLULAR STUDIES TO THE
CLINIC
T2 - NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS
WITH MATERIALS AND ATOMS
TI - NUCLEAR MICROSCOPY IN TRACE-ELEMENT BIOLOGY - FROM CELLULAR STUDIES TO THE
CLINIC
VL - 77
Y2 - 5
ID - 8882
ER -
TY - JOUR
AB - A 13-year-old Thoroughbred/Oldenburg gelding presented as a tertiary referral
for a 6-year history of immune-mediated keratitis (IMMK) after the development of
corneal and limbal nodules in the presence of medical therapy. A keratectomy
extending to the limbus was performed to remove the affected cornea and limbal
nodules. Two corneal-limbal samples were submitted for histopathology and initially
diagnosed as marked lympho-histiocytic stromal keratitis with epithelial
hyperplasia, keratinisation and keratinocyte apoptosis consistent with IMMK.
Additional testing with immunohistochemical staining for CD3, CD20, PAX5 and IBA1
revealed large, atypical B-lymphocytes on a background of histiocytes and mature T-
lymphocytes, with concern for T-cell-rich large B-cell lymphoma. PCR antigen
receptor rearrangement clonality testing was also performed and was diagnostic for
large B-cell lymphoma. Polymerase chain reaction (PCR) for equine herpesvirus 5
(EHV-5) was positive. Following keratectomy, a short course of neomycin/polymyxin
B/dexamethasone ophthalmic ointment was prescribed; no other treatment nor adjunct
therapy was performed. Eighteen months post-operation, the case remains visual and
comfortable on no medications. © 2022 EVJ Ltd.
AN - rayyan-553780865
AU - Lisankis, A. P.
AU - Beavis, B. B.
AU - Weigt, A. K.
AU - Nunnery, C.
DO - 10.1111/eve.13725
IS - 5
KW - B-cell lymphoma
equine herpesvirus 5
horse
immune-mediated keratitis
neoplastic transformation
atropine
CD20 antigen
CD3 antigen
detomidine
dexamethasone plus neomycin plus polymyxin B
diclofenac
flunixin meglumine
lidocaine
lymphocyte antigen receptor
mepivacaine
ofloxacin
proxymetacaine
sulfadiazine
sulfadiazine silver
tacrolimus
transcription factor PAX5
trimethoprim
xylazine
animal tissue
apoptosis
Article
B cell lymphoma
B lymphocyte
blepharospasm
conjunctival hyperemia
cornea limbus
edema
epithelium hyperplasia
Equid herpesvirus 1
equid herpesvirus 5
gelding
histiocyte
histopathology
immune mediated keratitis
keratectomy
keratinocyte
keratitis
nonhuman
Oldenburg Burnout Inventory
remission
T lymphocyte
tertiary care center
Thoroughbred horse
ulcer
Lymphoma, T-Cell
Lymphoma, B-Cell
PY - 2023
SP - e346-e351
ST - A case of malignant transformation of equine immune-mediated keratitis to B-
cell lymphoma
T2 - Equine Veterinary Education
TI - A case of malignant transformation of equine immune-mediated keratitis to B-
cell lymphoma
85141703716&doi=10.1111%2feve.13725&partnerID=40&md5=b3b0615acafbd86492f396e51ec9aa
fa
VL - 35
ID - 8883
ER -
TY - JOUR
AB - Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are
severe adverse drug reactions, characterized by a low incidence but high mortality,
initially described as separate entities, but today considered variants of the same
pathologic process and differing only for severity. The majority of cases appear to
be related to idiosyncratic drug reactions. The drugs most commonly involved are:
antibiotics such as sulfonamides, β-lactam, tetracyclines and quinolones;
anticonvulsants such as phenytoin, phenobarbital and carbamazapine; antiretroviral
drugs; nonsteroidal anti-inflammatory drugs, allopurinol. There is common agreement
to consider TEN as the manifestation of a disregulated immune reaction against
epithelial cells. During the first stages of TEN, apoptosis mediates keratinocyte
death and the pivotal role of Fas-FasL pathway activation during TEN is undoubted.
T cell cytotoxicity, demonstrated during TEN, has been shown to be mediated by the
perforin-granzyme pathway. It seems, also, clear that a peculiar cytokine pattern
plays an important role in TEN pathogenesis. The cutaneous findings result in an
acute macular erythematous rash with bullae. These lesions rapidly exhibit
Nikolsky's sign and a separation of large sheets of epidermis from the dermis and a
subsequent localised shedding develops rapidly, which can become very extensive.
When feasible, admission in burn or intensive care unit, positioning the patients
in air-fluidised beds, is universally considered crucial in TEN treatment. The
prompt withdrawal of the suspected drug, fluid and electrolyte replacement and
topical wound care are the first line of therapy. The use of corticosteroids has
been abandoned and the role of immunosuppressants, despite some success, is not
well defined and is not considered as a standard. A trial comparing thalidomide
versus placebo in TEN patients was suspended because mortality rate increased in
the treated group. Infliximab, a chimeric monoclonal antibody to TNF-α, has been
administered to a patient, in single infusion, with a favourable outcome.
Plasmapheresis is reported to lead to some success in TEN treatment, with
improvement of clinical conditions and high percentage of survival. Different
authors reported good results in terms of decreasing mortality and morbidity or
improving clinical conditions of the use of human intravenous immunoglobulins
(IVIGs). Regardless, the true utility of this treatment remains controversial. In
2005, the authors (ML and RC), dealing with a number of severe TEN cases, proposed
a new protocol based on the combination of these last two techniques reporting
their preliminary results in the treatment of severe TEN patients. © 2009 Elsevier
Ltd and ISBI.
AN - rayyan-553780866
AU - Lissia, M.
AU - Mulas, P.
AU - Bulla, A.
AU - Rubino, C.
DO - 10.1016/j.burns.2009.06.213
IS - 2
KW - Drug reactions
IVIG
Lyell's disease
Plasmapheresis
SCORTEN
Stevens-Johnson's syndrome
Epidermal Necrolysis, Toxic
Humans
Immunosuppressive Agents
Skin
biobrane
corticosteroid
cyclophosphamide
cyclosporin
immunoglobulin
infliximab
methylprednisolone
sulfadiazine silver
sulfonamide
thalidomide
tumor necrosis factor alpha antibody
antibiotic therapy
burn
clinical trial
Crohn disease
drug hypersensitivity
graft rejection
graft versus host reaction
human
immunopathology
plasmapheresis
review
skin injury
Stevens Johnson syndrome
total parenteral nutrition
toxic epidermal necrolysis
wound care
wound dressing
wound infection
Stevens-Johnson Syndrome
PY - 2010
SP - 152-163
ST - Toxic epidermal necrolysis (Lyell's disease)
T2 - Burns
TI - Toxic epidermal necrolysis (Lyell's disease)
76349090218&doi=10.1016%2fj.burns.2009.06.213&partnerID=40&md5=5f1da0ed1d6d21ed8c41
2f5bab150434
VL - 36
ID - 8884
ER -
TY - JOUR
AB - Group 3 innate lymphoid cells (ILC3) promote lymphoid organogenesis and
potentiate immune responses against bacterial infection. However, how ILC3 cells
are developed and maintained is still unclear. Here, we show that carboxypeptidase
CCP2 is highly expressed in common helper-like innate lymphoid progenitors, the
progenitor of innate lymphoid cells, and CCP2 deficiency increases ILC3 numbers.
Interleukin-7 receptor subunit alpha (IL-7Rα) is identified as a substrate of CCP2
for deglutamylation, and IL-7Rα polyglutamylation is catalyzed by polyglutamylases
TTLL4 and TTLL13 in common helper-like innate lymphoid progenitors. IL-7Rα
polyglutamylation triggers STAT5 activation to initiate transcription factor Sall3
expression in common helper-like innate lymphoid progenitors, which drives ILC3
cell differentiation. Moreover, Ttll4 -/- or Ttll13 -/- mice have reduced IL-7Rα
polyglutamylation and Sall3 expression in common helper-like innate lymphoid
progenitors. Importantly, mice with IL-7Rα E446A mutation have reduced Sall3
expression and ILC3 population. Thus, polyglutamylation and deglutamylation of IL-
7Rα tightly controls the development and effector functions of ILC3s. © 2017 The
Author(s).
AN - rayyan-553780867
AU - Liu, B.
AU - Ye, B.
AU - Zhu, X.
AU - Huang, G.
AU - Yang, L.
AU - Zhu, P.
AU - Du, Y.
AU - Wu, J.
AU - Meng, S.
AU - Tian, Y.
AU - Fan, Z.
DO - 10.1038/s41467-017-00235-x
IS - 1
KW - Animals
Glutamic Acid
Granzymes
Homeodomain Proteins
Immunity, Innate
Lymphocytes
Mice
Peptide Synthases
Receptors, Interleukin-7
Transcription Factors
Mus
carboxypeptidase
carboxypeptidase CCP2
interleukin 22
interleukin 23
interleukin 7
interleukin 7 receptor alpha
messenger RNA
short hairpin RNA
STAT3 protein
STAT5 protein
transcription factor sall3
unclassified drug
glutamic acid
granzyme
Gzmc protein, mouse
homeodomain protein
interleukin 7 receptor
interleukin-7 receptor, alpha chain
peptide synthase
Sall3 protein, mouse
bacterial disease
cells and cell components
enzyme activity
gene expression
immune response
infectivity
mutation
rodent
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bacterial load
catalysis
cell count
cell lysate
cell maturation
chromatin immunoprecipitation
comparative study
controlled study
feeder cell
female
flow cytometry
gel mobility shift assay
genetic transfection
group 3 innate lymphoid cell
immunoblotting
immunofluorescence test
in vitro study
inflammatory cell
luciferase assay
lymphoid cell
mouse
nonhuman
promoter region
protein expression
sequence analysis
silver staining
animal
cytology
genetics
immunology
innate immunity
lymphocyte
metabolism
physiology
Transcriptional Activation
PY - 2017
ST - IL-7Rα glutamylation and activation of transcription factor Sall3 promote
group 3 ILC development
T2 - Nature Communications
TI - IL-7Rα glutamylation and activation of transcription factor Sall3 promote
group 3 ILC development
85027219970&doi=10.1038%2fs41467-017-00235-
x&partnerID=40&md5=4ff2cb0e6595e942e9ae4a59628afd08
VL - 8
ID - 8885
ER -
TY - JOUR
AB - Background: PD-1/PD-L1 immune checkpoint inhibitors are currently the most
commonly utilized agents in clinical practice, which elicit an immunostimulatory
response to combat malignancies. However, all these inhibitors are currently
administered via injection using antibody-based therapies, while there is a growing
need for oral alternatives. Methods: This study has developed and synthesized
exosome-wrapped gold–peptide nanocomplexes with low immunogenicity, which can
target PD-L1 and activate antitumor immunity in vivo through oral absorption. The
SuperPDL1exo was characterized by transmission electron microscopy (TEM), dynamic
light scattering (DLS), Fourier transform infrared (FTIR), X-ray photoelectron
spectroscopy (XPS), and gel silver staining. The transmembrane ability of
SuperPDL1exo was evaluated by flow cytometry and immunofluorescence. Cell viability
was determined using the Cell Counting Kit-8 (CCK-8) assay. ELISA experiments were
conducted to detect serum and tissue inflammatory factors, as well as serum
biochemical indicators. Tissue sections were stained with H&E for the evaluation of
the safety of SuperPDL1exo. An MC38 colon cancer model was established in
immunocompetent C56BL/6 mice to evaluate the effects of SuperPDL1exo on tumor
growth in vivo. Immunohistochemistry (IHC) staining was performed to detect
cytotoxicity factors such as perforin and granzymes. Results: First, SuperPDL1 was
successfully synthesized, and milk exosome membranes were encapsulated through
ultrasound, repeated freeze–thaw cycles, and extrusion, resulting in the synthesis
of SuperPDL1exo. Multiple characterization results confirmed the successful
synthesis of SuperPDL1exo nanoparticles. Furthermore, our data demonstrated that
SuperPDL1exo exhibited excellent colloidal stability and superior cell
transmembrane ability. In vitro and in vivo experiments revealed that SuperPDL1exo
did not cause damage to multiple systemic organs, demonstrating its good
biocompatibility. Finally, in the MC38 colon cancer mouse model, it was discovered
that SuperPDL1exo could inhibit the progression of colon cancer, and this tumor-
suppressive effect was mediated through the activation of tumor-specific cytotoxic
T lymphocyte (CTL)-related immune responses. Conclusion: This study has
successfully designed and synthesized an oral nanotherapeutic, SuperPDL1exo, which
demonstrates small particle size, excellent colloidal stability, transmembrane
ability in tumor cells, and biocompatibility. In vivo experiments have shown that
it effectively activates T-cell immunity and exerts antitumor effects. Copyright ©
2023 Liu, Wang, You, Ma, Sun, She, He and Yang.
AN - rayyan-553780869
AU - Liu, D.
AU - Wang, J.
AU - You, W.
AU - Ma, F.
AU - Sun, Q.
AU - She, J.
AU - He, W.
AU - Yang, G.
DO - 10.3389/fimmu.2023.1228581
KW - anti-tumor
immunotherapy
milk exosome
peptide
supramolecular nanospheres
Animals
B7-H1 Antigen
Cell Line, Tumor
Colonic Neoplasms
Immunotherapy
Mice
Peptides
Programmed Cell Death 1 Receptor
d peptide
fluorescein isothiocyanate
granzyme
nanoparticle
nanosphere
perforin
programmed death 1 ligand 1
unclassified drug
absorption
animal experiment
animal model
animal tissue
apoptosis
Article
biocompatibility
cancer growth
cancer immunotherapy
cell infiltration
cell viability
cellular immunity
clinical practice
colon cancer
colorectal cancer
confocal laser scanning microscopy
cytotoxic T lymphocyte
cytotoxicity
encapsulation
enzyme activity
excitation
exosome
flow cytometry
Fourier transform
immune response
immunofluorescence
immunogenicity
immunostimulation
in vivo study
milk
mouse
nanomedicine
nonhuman
oral absorption
particle size
silver staining
synthesis
T lymphocyte
tissue section
tumor growth
tumor immunity
tumor volume
ultrasound
zeta potential
animal
colon tumor
metabolism
procedures
tumor cell line
Endothelin-1
Doxazosin
PY - 2023
ST - A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction
for tumor immunotherapy
TI - A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction
for tumor immunotherapy
85166045120&doi=10.3389%2ffimmu.2023.1228581&partnerID=40&md5=8e77e417aac5649493e5d
f8c00b17d04
VL - 14
ID - 8886
ER -
TY - JOUR
AB - Manufactured zinc oxide nanoparticles (Nano-ZnO) are being used increasingly
in many fields owing to their excellent physicochemical properties. Consequently,
biosecurity has become a growing concern for human health and the environment. In
the present study. Nano-ZnO neurotoxidty was investigated in vivo and in vitro. in
vivo results showed that Nano-ZnO particles delivered through intranasal
instillation were translocated to the brain, specifically deposited in the
olfactory bulb, hippocampus, striatum, and cerebral cortex, and caused
ultrastructural changes, oxidative damage, inflammatory responses, and
histopathological damages there, which may be important for inducing Nano-ZnO
neurotoxidty. Further in vitro studies on PC12 cell line illustrated that exposure
to Nano-ZnO for 6 h affected cell morphology, decreased cell viability, increased
lactate dehydrogenase and oxidative stress activity levels, impaired mitochondrial
function, and disturbed the cell cycle. In addition, Nano-ZnO could destroy
neuronal structure by affecting cytoskeleton proteins (tubulin-alpha, tubulin-beta
and NF-H), resulting in the interruption of connection between nerve cells, which
lead to nervous system function damage. Meanwhile, Nano-ZnO could induce neuronal
repair and regeneration disorders by affecting the growth-related protein GAP43 and
delayed neurotoxidty by affecting the calcitun/caldum-regulated kinase
(CAMK2A/CAMK2B protein) signaling pathway. (C) 2019 Elsevier B.V. All rights
reserved.
AN - rayyan-553780870
AU - Liu, H. L.
AU - Yang, H. L.
AU - Fang, Y. J.
AU - Li, K.
AU - Tian, L.
AU - Li, X. H.
AU - Zhang, W.
AU - Tan, Y. Z.
AU - Lai, W. Q.
AU - Bian, L. P.
AU - Lin, B. C.
AU - Xi, Z. G.
DO - 10.1016/j.scitotenv.2019.135809
KW - Brain
Biological Markers
Zinc
PY - 2020
SN - 0048-9697 1879-1026
ST - Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional
brain regions and dopaminergic neurons
T2 - SCIENCE OF THE TOTAL ENVIRONMENT
TI - Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional
brain regions and dopaminergic neurons
VL - 705
Y2 - 2 y3 - 25
ID - 8887
ER -
TY - JOUR
AB - Zinc oxide, titanium dioxide and silver nanoparticles are used as
sterilization materials to enhance the performance of disinfectants. Here, the
toxicological effects on the liver, spleen, thymus gland, immune function and
inflammatory responses in rats induced by these nanoparticles were investigated
after intratracheal instillation in male Wistar rats. Moreover, the relationships
between the particle size, particle crystalline structure, chemical composition,
chemical stability and toxicological effects of these typical nanoparticles in rats
were explored. Exposure to nanoparticles increased the oxidative stress level in
peripheral blood and the homogenates of the liver, spleen and thymus as well as
disorders in regulating the cytokine network and blood cell count in the peripheral
blood. Furthermore, the histopathological study revealed that pulmonary exposure to
nanoparticles produced persistent, progressive liver inflammatory responses and
cell necrosis, while no observable damage was found in the kidney, thymus gland or
spleen tissue from the experimental groups. Our results demonstrate that oxidative
stress might be important for inducing the toxic effects of these nanoparticles,
and three nanoparticles can influence the immune function of rats. A comparative
analysis of the toxic effects of nanomaterials demonstrated significant
differences. Nano-ZnO induced the most significant toxicity, whereas nano-TiO2
induced the least. Particle composition and chemical stability probably played a
primary role in the toxicological effects of different nanoparticles. © 2015 The
AN - rayyan-553780871
AU - Liu, H. L.
AU - Yang, H. L.
AU - Lin, B. C.
AU - Zhang, W.
AU - Tian, L.
AU - Zhang, H. S.
AU - Xi, Z. G.
DO - 10.1039/c4tx00154k
IS - 2
KW - Rattus
Rattus norvegicus
silver nanoparticle
titanium dioxide
zinc oxide
animal experiment
Article
blood cell count
cell death
controlled study
crystal structure
double antibody sandwich enzyme linked immunosorbent assay
erythrocyte count
histopathology
immune response
inflammation
instrument sterilization
kidney
leukocyte count
liver homogenate
male
nonhuman
oxidative stress
particle size
priority journal
randomized controlled trial
rat
thymus
toxicity testing
Wistar rat
Rats
Oxidative Stress
Inflammation
PY - 2015
SP - 486-493
ST - Toxic effect comparison of three typical sterilization nanoparticles on
oxidative stress and immune inflammation response in rats
T2 - Toxicology Research
TI - Toxic effect comparison of three typical sterilization nanoparticles on
oxidative stress and immune inflammation response in rats
84923870106&doi=10.1039%2fc4tx00154k&partnerID=40&md5=4e1c4f1e8c5fd2cfeaa5666b970c3
b08
VL - 4
ID - 8888
ER -
TY - JOUR
AB - Silver, zinc oxide, and titanium dioxide nanoparticles are used as
sterilisation materials to enhance the performance of disinfectants. We
investigated the respiratory tract immune toxicity (" immunotoxicity" ) of these
nanoparticles in vivo and in vitro, and we explored the relationships between
particle size, particle shape, chemical composition, chemical stability and the
toxicological effects of these typical nanoparticles in rats. In vivo, the rats
were exposed to nanoparticles by intratracheal instillation. Exposure to
nanoparticles caused an increase in oxidative injury to the lungs and disorders in
regulating the cytokine network, which were detected in the bronchoalveolar lavage
fluid, suggesting that oxidative stress might be important for inducing the
respiratory immunotoxicity of nanoparticles. In vitro, the phagocytic function of
alveolar macrophages (AMs) was dose-dependently reduced by nanoparticles, and ZnO
nanoparticles induced greater cytotoxicity than the silver and titanium-dioxide
nanoparticles, which were coincident with the results of multiple measurements,
such as a cell viability assay by WST-8 and LDH measurements. Comparative analyses
demonstrated that particle composition and chemical stability most likely had a
primary role in the biological effects of different nanoparticles. © 2013 Elsevier
B.V.
AN - rayyan-553780872
AU - Liu, H.
AU - Yang, D.
AU - Yang, H.
AU - Zhang, H.
AU - Zhang, W.
AU - Fang, Y.
AU - Lin, Z.
AU - Tian, L.
AU - Lin, B.
AU - Yan, J.
AU - Xi, Z.
DO - 10.1016/j.jhazmat.2013.01.046
IS - 1
KW - Comparative study
Respiratory tract immune toxicity
Titanium dioxide nanoparticles
Zinc oxide nanoparticles
Animals
Cell Survival
Cytokines
Glutathione
Macrophages, Alveolar
Malondialdehyde
Metal Nanoparticles
Nitric Oxide
Phagocytosis
Rats
Rats, Wistar
Respiratory System
Silver
Sterilization
Tetrazolium Salts
Titanium
Zinc Oxide
Nanoparticles
Oxides
Titanium dioxide
Toxicity
Zinc oxide
disinfectant agent
interleukin 1
interleukin 6
nanoparticle
silver nanoparticle
tetrazolium
titanium dioxide
unclassified drug
zinc oxide
Comparative studies
Immune toxicities
comparative study
disinfection
immune response
injury
nanotechnology
particle size
pollution exposure
respiratory disease
rodent
silver
sterilization
titanium
toxicity
toxicology
zinc
animal cell
animal experiment
animal model
article
cell viability
controlled study
cytotoxicity
exposure
immunotoxicity
in vitro study
in vivo study
instrument sterilization
lung lavage
measurement
nonhuman
oxidative stress
phagocyte
rat
respiratory tract disease
respiratory tract immune toxicity
Zinc
PY - 2013
SP - 478-486
ST - Comparative study of respiratory tract immune toxicity induced by three
sterilisation nanoparticles: Silver, Zinc oxide and titanium dioxide
T2 - Journal of Hazardous Materials
TI - Comparative study of respiratory tract immune toxicity induced by three
sterilisation nanoparticles: Silver, Zinc oxide and titanium dioxide
84873725133&doi=10.1016%2fj.jhazmat.2013.01.046&partnerID=40&md5=84fc7599aca3511691
cfbb8a26f5b895
VL - 248
ID - 8889
ER -
TY - JOUR
AB - Purpose: Radiation recall dermatitis (RRD) is a rare complication that occurs
after completion of radiation therapy (RT) and initiation of a precipitating agent,
most commonly chemotherapeutic medications. Various theories attempt to explain the
mechanism, including activation of the body's inflammatory pathways through
nonimmune activation. Likewise, radiation-induced organizing pneumonia (RIOP) is an
infrequent but potentially life-threatening complication of RT that, while not
fully understood, is suspected to be partly an autoimmune reaction. Patient: We
present the case of a 71-year-old female with a history of type 2 diabetes
mellitus, hypothyroidism, interstitial cystitis, and osteoarthritis who presented
with clinical stage T1N0M0 ER+/PR-/HER2- invasive ductal carcinoma of the lower
outer quadrant of the left breast, for which she underwent left segmental
mastectomy and sentinel lymph node biopsy followed by completion axillary lymph
node dissection. Her final pathologic stage was T1N1M0. Result: The patient
developed RRD and later RIOP following receipt of radiation and chemotherapy, which
resolved with steroid administration. Conclusions: The rarity of both RRD and RIOP
occurring in a patient, as in our case, suggests a shared pathophysiology behind
these two complications. As both reactions involve some degree of inflammation and
respond to corticosteroids, it seems likely that the etiologies of RRD and RIOP lie
within the inflammatory pathway. However, further investigation should evaluate the
frequency, duration, and triggering of concomitant RRD and RIOP. © 2020 S. Karger
AG. All rights reserved.
AN - rayyan-553780873
AU - Liu, I. C.
AU - Giap, F.
AU - Mailhot-Vega, R. B.
AU - Bradley, J. A.
AU - Mendenhall, N. P.
AU - Okunieff, P.
AU - Lu, L.
AU - Jantz, M. A.
AU - Daily, K.
AU - Spiguel, L.
AU - Lockney, N. A.
DO - 10.1159/000508493
IS - 2
KW - Dermatitis
Radiation recall
Radiation toxicity
Radiation-induced organizing pneumonia
azithromycin
benzonatate
cefalexin
cotrimoxazole
cyclophosphamide
docetaxel
letrozole
miaderm
paracetamol
prednisone
radioprotective agent
sulfadiazine silver
adjuvant chemotherapy
aged
Article
breast cancer-related lymphedema
breast carcinoma
breast discomfort
breast disease
breast radiotherapy
breast tenderness
bronchoscopy
burning sensation
cancer staging
case report
clinical article
computed tomographic angiography
coughing
drug dose increase
drug dose reduction
drug withdrawal
dyspnea
eosinophilia
erythema
fatigue
female
fever
follow up
heat
human
human tissue
hyperglycemia
hypothyroidism
insomnia
interstitial cystitis
intraductal carcinoma
leukocyte differential count
lung biopsy
lung lavage
lymph node dissection
lymph vessel metastasis
lymphocytopenia
monocytosis
multiple cycle treatment
organizing pneumonia
osteoarthritis
oxygen supply
partial mastectomy
physiotherapy
priority journal
radiation dose
radiation pneumonia
sentinel lymph node biopsy
thorax radiography
Radiotherapy
Pneumonia
PY - 2020
SP - 875-882
ST - Concomitant Radiation Recall Dermatitis and Organizing Pneumonia following
Breast Radiotherapy: A Case Report
T2 - Case Reports in Oncology
TI - Concomitant Radiation Recall Dermatitis and Organizing Pneumonia following
Breast Radiotherapy: A Case Report
85089155124&doi=10.1159%2f000508493&partnerID=40&md5=689212058e401e9611d757c266bce1
f1
VL - 13
ID - 8890
ER -
TY - JOUR
AB - Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the
potential exposure of workers, consumers, and scientists to these particles has
increased. This potential for exposure has attracted extensive attention in the
science community. Many studies have examined the toxicological profile of ZnO NPs
in the immune system, digestive system, however, information regarding the toxicity
of ZnO NPs in the nervous system is scarce. In this study, we detected the
cytotoxicity of two types of ZnO NPs of various sizes - ZnOa NPs and ZnOb NPs - and
we characterized the shedding ability of zinc ions within culture medium and the
cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-
induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis
and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnOb
NPs induced more significant toxic responses than ZnOa NPs in SHSY5Y cells. In
conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent
manner, which can probably be attributed to their ion-shedding ability.
AN - rayyan-553780874
AU - Liu, J.
AU - Kang, Y. Y.
AU - Yin, S. H.
AU - Song, B.
AU - Wei, L. M.
AU - Chen, L. J.
AU - Shao, L. Q.
DO - 10.2147/IJN.S149070
KW - Humanities
Humanism
Humans
Zinc
Neuroblastoma
Zinc Oxide
PY - 2017
SN - 1178-2013
SP - 8085-8099
ST - Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y
cells in a size-dependent manner
TI - Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y
cells in a size-dependent manner
VL - 12
ID - 8891
ER -
TY - JOUR
AB - The use of contact lenses for the early treatment of bacterial or fungal
keratitis has become a new research focus. Two main requirements of the therapeutic
contact lenses are antimicrobial ability and visible light transmittance. Silver
nanoparticles (AgNPs), as a nonspecific antimicrobial component, have been loaded
onto contact lenses for the treatment of bacterial and fungal keratitis. Recently,
it was reported that, via a simple immersion method, AgNPs can be synthesized and
fixed onto the surface of polydopamine (PDA)-coated materials. However, in this
study, we found that the above traditional method has the disadvantages of poor
AgNP loading and low visible light transmittance, which could be induced by a
limited amount of phenolic hydroxyl groups on and second oxidation of the PDA
coating, respectively. To overcome these disadvantages, in this paper, we provided
a facile and novel method to robustly bind multilayer-AgNPs on contact lens
surfaces by using dopamine as a reducing agent and bioglue. In comparing with the
monolayer-AgNP-loaded contact lenses fabricated by the traditional method, the
multilayer-AgNP-loaded contact lenses had excellent antimicrobial ability and
better visible light transmittance. Moreover, the multilayer-AgNP-loaded contact
lens had low cytotoxicity to human corneal epithelial cells and anti-inflammation
properties. Furthermore, the shortcoming of decreasing visible light transmittance
induced by excess adherence of AgNPs on the multilayer-AgNP-loaded contact lens was
alleviated by decreasing the size of AgNPs through altering the concentration of
dopamine and AgNO(3). Contact lenses loaded with small AgNPs (Ag@PDA-2.5, diameter
≈ 25-50 nm) had approximately the same Ag(+) release and antimicrobial abilities,
but significantly better visible light transmittance and anti-inflammatory
properties than the contact lenses loaded with large AgNPs (Ag@PDA-5, diameter ≈
50-75 nm). After that, in vivo testing indicated the promising therapeutic strategy
of multilayer-AgNP-loaded contact lenses (Ag@PDA-2.5) for early bacterial keratitis
and fungal keratitis. In addition, PDA coating could provide reactive sites to
immobilize other biomolecules or drugs on this multilayer-AgNP-loaded contact lens
for further combination therapies in treating bacterial or fungal keratitis.
Finally, the stability of the visible light transmittance of the multilayer-AgNP-
loaded contact lens was detected. The visible light transmittance of Ag@PDA-2.5 was
weakened after being cultured with an extremely high concentration of bacteria,
while it was stable in the moderate work environment. Though PDA coating had been
wildly used to modify implantation devices, however, few studies about PDA coating
modified contact lenses have been reported so far. Therefore, this research also
provides an important basis for using PDA coating to modify a therapeutic contact
lens.
AN - rayyan-553782372
AU - Liu, X.
AU - Chen, J.
AU - Qu, C.
AU - Bo, G.
AU - Jiang, L.
AU - Zhao, H.
AU - Zhang, J.
AU - Lin, Y.
AU - Hua, Y.
AU - Yang, P.
AU - Huang, N.
AU - Yang, Z.
DO - 10.1021/acsbiomaterials.7b00977
IS - 5
J2 - ACS Biomater Sci Eng
LA - eng
N1 - Institute of Biomaterials and Surface Engineering, Key Lab for Advanced
Technologies of Materials, Ministry of Education, Southwest Jiaotong University,
No. 111 of the North First Section of Second Ring Road, Chengdu, CN 610031, China.;
Sichuan Key Laboratory for Disease Gene Study, Sichuan Academy of Medical Sciences
& Sichuan Provincial People's Hospital, University of Electronic Science and
Technology of China, No. 32 of the West Second Section of First Ring Road, Chengdu,
CN 610072, China.; Department of Laboratory Medicine, Sichuan Academy of Medical
Sciences and Sichuan Provincial People's Hospital, University of Electronic Science
and Technology of China, No. 32 of the West Second Section of First Ring Road,
Chengdu, CN 610072, China.; Institute of Biomaterials and Surface Engineering, Key
Lab for Advanced Technologies of Materials, Ministry of Education, Southwest
Jiaotong University, No. 111 of the North First Section of Second Ring Road,
Chengdu, CN 610031, China.; Department of Ophthalmology, Sichuan Academy of Medical
Sciences & Sichuan Provincial People's Hospital, University of Electronic Science
Chengdu, CN 610072, China.; Sichuan Key Laboratory for Disease Gene Study, Sichuan
Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of
Electronic Science and Technology of China, No. 32 of the West Second Section of
First Ring Road, Chengdu, CN 610072, China.; Department of Laboratory Medicine,
Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital,
University of Electronic Science and Technology of China, No. 32 of the West Second
Section of First Ring Road, Chengdu, CN 610072, China.; Institute of Biomaterials
and Surface Engineering, Key Lab for Advanced Technologies of Materials, Ministry
of Education, Southwest Jiaotong University, No. 111 of the North First Section of
Second Ring Road, Chengdu, CN 610031, China.; School of Medicine, University of
Electronic Science and Technology of China, No. 2006, Xiyuan Ave West Hi-Tech Zone,
Chengdu, CN 611731, China.; Department of Ophthalmology, Sichuan Academy of Medical
Sciences & Sichuan Provincial People's Hospital, University of Electronic Science
Chengdu, CN 610072, China.; Department of Laboratory Medicine, Sichuan Academy of
Medical Sciences and Sichuan Provincial People's Hospital, University of Electronic
Science and Technology of China, No. 32 of the West Second Section of First Ring
Road, Chengdu, CN 610072, China.; Department of Laboratory Medicine, Sichuan
Academy of Medical Sciences and Sichuan Provincial People's Hospital, University of
Electronic Science and Technology of China, No. 32 of the West Second Section of
First Ring Road, Chengdu, CN 610072, China.; Institute of Biomaterials and Surface
Engineering, Key Lab for Advanced Technologies of Materials, Ministry of Education,
Southwest Jiaotong University, No. 111 of the North First Section of Second Ring
Road, Chengdu, CN 610031, China.; Institute of Biomaterials and Surface
Engineering, Key Lab for Advanced Technologies of Materials, Ministry of Education,
Southwest Jiaotong University, No. 111 of the North First Section of Second Ring
Road, Chengdu, CN 610031, China.; Sichuan Key Laboratory for Disease Gene Study,
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,
University of Electronic Science and Technology of China, No. 32 of the West Second
Section of First Ring Road, Chengdu, CN 610072, China.
PY - 2018
SP - 1568-1579
ST - A Mussel-Inspired Facile Method to Prepare Multilayer-AgNP-Loaded Contact
Lens for Early Treatment of Bacterial and Fungal Keratitis
T2 - ACS biomaterials science & engineering
TI - A Mussel-Inspired Facile Method to Prepare Multilayer-AgNP-Loaded Contact
Lens for Early Treatment of Bacterial and Fungal Keratitis
VL - 4
Y2 - 5 y3 - 14
ID - 10279
ER -
TY - JOUR
AB - The secretome secreted by stem cells and bioactive material has emerged as a
promising therapeutic choice for traumatic brain injury (TBI). We aimed to
determine the effect of 3D-printed collagen/chitosan/secretome derived from human
umbilical cord blood mesenchymal stem cells scaffolds (3D-CC-ST) on the injured
tissue regeneration process. 3D-CC-ST was performed using 3D printing technology at
a low temperature (-20°C), and the physical properties and degeneration rate were
measured. The utilization of low temperature contributed to a higher
cytocompatibility of fabricating porous 3D architectures that provide a homogeneous
distribution of cells. Immediately after the establishment of the canine TBI model,
3D-CC-ST and 3D-CC (3D-printed collagen/chitosan scaffolds) were implanted into the
cavity of TBI. Following implantation of scaffolds, neurological examination and
motor evoked potential detection were performed to analyze locomotor function
recovery. Histological and immunofluorescence staining were performed to evaluate
neuro-regeneration. The group treated with 3D-CC-ST had good performance of
behavior functions. Implanting 3D-CC-ST significantly reduced the cavity area,
facilitated the regeneration of nerve fibers and vessel reconstruction, and
promoted endogenous neuronal differentiation and synapse formation after TBI. The
implantation of 3D-CC-ST also markedly reduced cell apoptosis and regulated the
level of systemic inflammatory factors after TBI. © 2022 The Author(s). Published
by Oxford University Press.
AN - rayyan-553780878
AU - Liu, X.
AU - Zhang, G.
AU - Wei, P.
AU - Zhong, L.
AU - Chen, Y.
AU - Zhang, J.
AU - Chen, X.
AU - Zhou, L.
DO - 10.1093/rb/rbac043
KW - canines
chitosan
collagen
low temperature extrusion 3D printing
secretome
3D modeling
Brain
Cell culture
Cell death
Collagen
Image reconstruction
Neurons
Scaffolds (biology)
Stem cells
Temperature
Three dimensional computer graphics
Tissue regeneration
cell protein
interleukin 10
interleukin 6
microtubule associated protein 2
tuj 1 protein
unclassified drug
3-D printing
3D-printing
Canine
Chitosan scaffold
Low temperature extrusion
Low temperature extrusion 3d printing
Lows-temperatures
Neural-networks
Secretome
Traumatic Brain Injuries
animal experiment
animal model
animal tissue
Article
brain histology
brain tissue
canine model
cell adhesion
cell proliferation
cell survival
cell viability
controlled study
female
human
human cell
immunofluorescence
implantation
in vitro study
in vivo study
locomotion
low temperature
Masson staining
motor evoked potential
MTT assay
myelin sheath
nerve cell differentiation
nerve fiber regeneration
neuroapoptosis
nonhuman
phase contrast microscopy
porosity
remyelinization
silver staining
stereomicroscopy
synapse
three dimensional printing
tissue engineering
tissue regeneration
umbilical cord mesenchymal stem cell
water absorption
Young modulus
3D printers
Neural Networks (Computer)
Nerve Net
Brain Injuries
PY - 2022
ST - Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs
scaffolds for efficient neural network reconstruction in canines with traumatic
brain injury
T2 - Regenerative Biomaterials
TI - Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs
scaffolds for efficient neural network reconstruction in canines with traumatic
brain injury
85139950094&doi=10.1093%2frb
%2frbac043&partnerID=40&md5=95b1dfea38e6a5140f37c84ef6c38475
VL - 9
ID - 8892
ER -
TY - JOUR
AB - The treatment of diabetic wounds remains a global challenge. Compared with
traditional wound dressings, there are higher requirements of antibacterial, anti-
inflammatory and pro-angiogenic effects in diabetic wound dressings. Furthermore,
it is desirable for dressings to self-adapt to wounds with different morphologies
without extra processes and stably (suitable adhesive and self-healing abilities)
provide a conducive environment for wound healing. Herein, we construct an
injectable and self-healing hydrogel through the combination of chitosan (CS) and
metal ions to efficiently improve infected and diabetic wound healing. Benefiting
from the amino and hydroxy groups, the CS molecular chains are cross-linked with
silver ions (Ag(+)) and copper ions (Cu(2+)) to promote the formation of the CS-Ag-
Cu hydrogel, which releases Ag(+) (an antibacterial agent) and Cu(2+) (an
angiogenic agent) over a prolonged period. Moreover, the hydrogel possesses
appropriate adhesive ability, good water absorption ability, antibacterial
capability and biocompatibility according to in vitro investigations. In vivo
experimental results further prove that the CS-Ag-Cu hydrogel can dramatically
accelerate tissue repair in a Staphylococcus aureus (S. aureus)-infected skin
incision model in normal rats and diabetic wounds.
AN - rayyan-553782336
AU - Liu, X.
AU - Zhou, S.
AU - Cai, B.
AU - Wang, Y.
AU - Deng, D.
AU - Wang, X.
DO - 10.1039/d2bm00224h
IS - 13
J2 - Biomater Sci
KW - Adhesives/pharmacology
Animals
*Chitosan/pharmacology
*Diabetes Mellitus
Hydrogels/pharmacology
Rats
Wound Healing
LA - eng
N1 - College of Chemistry, Nanchang University, Nanchang, Jiangxi, 330088, P.R.
China. wangxiaolei@ncu.edu.cn.; The National Engineering Research Center for
Bioengineering Drugs and the Technologies, Institute of Translational Medicine,
Nanchang University, Nanchang, Jiangxi, 330088, P.R. China.; College of Chemistry
and Chemical Engineering, Jinggangshan University, Ji'an, Jiangxi, 343009, P.R.
China.; The National Engineering Research Center for Bioengineering Drugs and the
Technologies, Institute of Translational Medicine, Nanchang University, Nanchang,
Jiangxi, 330088, P.R. China.; The National Engineering Research Center for
Bioengineering Drugs and the Technologies, Institute of Translational Medicine,
Nanchang University, Nanchang, Jiangxi, 330088, P.R. China.; The National
Engineering Research Center for Bioengineering Drugs and the Technologies,
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi,
330088, P.R. China.; Affiliated Eye Hospital of Nanchang University, Nanchang
University, Nanchang, Jiangxi, 330006, P.R. China.; College of Chemistry, Nanchang
University, Nanchang, Jiangxi, 330088, P.R. China. wangxiaolei@ncu.edu.cn.; The
National Engineering Research Center for Bioengineering Drugs and the Technologies,
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi,
330088, P.R. China.; College of Chemistry, Nanchang University, Nanchang, Jiangxi,
330088, P.R. China. wangxiaolei@ncu.edu.cn.; The National Engineering Research
Center for Bioengineering Drugs and the Technologies, Institute of Translational
Medicine, Nanchang University, Nanchang, Jiangxi, 330088, P.R. China.
PY - 2022
SP - 3480-3492
ST - An injectable and self-healing hydrogel with antibacterial and angiogenic
properties for diabetic wound healing
T2 - Biomaterials science
TI - An injectable and self-healing hydrogel with antibacterial and angiogenic
properties for diabetic wound healing
VL - 10
Y2 - 6 y3 - 28
ID - 10244
ER -
TY - JOUR
AB - The formulation and characterization of novel cardiovascular supplements and
drugs without any side effects are in both developed and developing countries
research priority. Myocardial ischemia-reperfusion causes to an inflammatory
response that leads more damage to apoptosis. Chronic and acute immune responses
elicited by myocardial ischemia is in the heart functional deterioration. In this
study, we formulated a modern cardioprotective protective drug by gold
nanoparticles containing Silybum marianum on isoproterenol-induced myocardial
ischemia mice by investigating the PPAR-gamma/NF-kappa B pathway. Determining the
antioxidant capacities of gold nanoparticles was done with the conventional free
radical scavenging test, i.e., DPPH in the presence of butylated hydroxytoluene as
the positive control. The gold nanoparticles were characterized by standard
physicochemical techniques, including FT-IR, FE-SEM, UV-Vis, and TEM. The
nanoparticles inhibited half of the DPPH molecules in the concentration of 72 mu
g/mL. In the in vivo design, to induce myocardial ischemia in C57BL/6 mice,
isoproterenol (40 mg/kg) was administered. The mice were randomly divided into five
groups: (1) control; (2) isoproterenol; (3-5) isoproterenol + gold nanoparticles at
different doses (10, 20 and 40 mu g/ml) and timings. After treatment, cardiac
function was evaluated by histochemical and biochemical analysis. Gold
nanoparticles treatment decreased the inflammatory milieu in the myocardial
ischemia mice heart, thereby blocking the proinflammatory cytokines upregulation
(IL-1 ss, TNF-alpha and IL-6). Also, gold nanoparticles treatment significantly
ameliorate ventricular wall ischemia, reduces the mortality incidence, and inhibits
the myocardial injury markers levels. In addition, gold nanoparticles
administration significantly prevents the typical ST segment depression. Treatment
with gold nanoparticles significantly suppresses the inflammation cytokines
expression and decrease cell death. The gold nanoparticles beneficial effects is
related to the normalization in PPAR-gamma and PPAR-gamma/NF-kappa B/I kappa B-
alpha/IKK alpha/ss phosphorylation gene expression. Gold nanoparticles exert
cardioprotective properties against isoproterenol-induced myocardial ischemia in
mice, which may associated to the PPAR-gamma activation and NF-kappa B signaling
inhibition.
AN - rayyan-553780880
AU - Liu, Y.
AU - Guo, J.
AU - Zhou, Z.
AU - Wu, Q. K.
AU - Jin, X.
AU - Wang, T.
DO - 10.1016/j.inoche.2023.110902
KW - Myocardial Ischemia
PY - 2023
SN - 1387-7003 1879-0259
ST - Protective properties of nanoparticles green-synthesized by plant on
myocardial ischemia
T2 - INORGANIC CHEMISTRY COMMUNICATIONS
TI - Protective properties of nanoparticles green-synthesized by plant on
myocardial ischemia
VL - 154
Y2 - 8
ID - 8893
ER -
TY - JOUR
AB - Background: Gold nanoparticles (AuNPs) have potential applications in the
treatment and diagnosis process, which are attributed to their biocompatibility and
high efficiency of drug delivery. In the current study, we utilized an extract of
Euphrasia o/ficinalis, a traditional folk medicine, to synthesize gold
nanoparticles (EO-AuNPs), and investigated their anti-inflammatory effects on
lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Materials and methods:
The AuNPs were synthesized from an ethanol extract of E. officinalis leaves and
characterized using several analytical techniques. Anti-inflammatory activities of
EO-AuNPs were detected by a model of LPS-induced upregulation of inflammatory
mediators and cytokines including nitric oxide (NO), inducible nitric oxide
synthase (iNOS), tumor necrosis factor-alpha (TNF-alpha), IL-1 beta, and IL-6 in
RAW 264.7 cells. The activation of nuclear factor (NF)-kappa B and Janus
kinase/signal transducer and activators of transcription (JAK/STAT) signaling
pathways was investigated by Western blot. Results: The results confirmed the
successful synthesis of AuNPs by E. officinalis. Transmission electron microscopy
images showed obvious uptake of EO-AuNPs and internalization into intracellular
membrane-bound compartments, resembling endosomes and lysosomes by RAW 264.7 cells.
Cell viability assays showed that EO-AuNPs exhibited little cytotoxicity in RAW
264.7 cells at 100 mu g/mL concentration after 24 hours. EO-AuNPs significantly
suppressed the LPS-induced release of NO, TNF-alpha, IL-1 beta, and IL-6 as well as
the expression of the iNOS gene and protein in RAW 264.7 cells. Further experiments
demonstrated that pretreatment with EO-AuNPs significantly reduced the
phosphorylation and degradation of inhibitor kappa B-alpha and inhibited the
nuclear translocation of NF-kappa B p65. In addition, EO-AuNPs suppressed LPS-
stimulated inflammation by blocking the activation of JAK/STAT pathway. Conclusion:
The synthesized EO-AuNPs showed anti-inflammatory activity in LPS-induced RAW 264.7
cells, suggesting they may be potential candidates for treating inflammatory-
mediated diseases.
AN - rayyan-553780881
AU - Liu, Y.
AU - Kim, S.
AU - Kim, Y. J.
AU - Perumalsamy, H.
AU - Lee, S.
AU - Hwang, E.
AU - Yi, T. H.
DO - 10.2147/IJN.S199781
KW - NF-kappa B
Macrophages
Inflammation
Lipopolysaccharides
PY - 2019
SN - 1178-2013
SP - 2945-2959
ST - Green synthesis of gold nanoparticles using Euphrasia officinalis leaf
extract to inhibit lipopolysaccharide-induced inflammation through NF-kappa B and
JAK/STAT pathways in RAW 264.7 macrophages
TI - Green synthesis of gold nanoparticles using Euphrasia officinalis leaf
extract to inhibit lipopolysaccharide-induced inflammation through NF-kappa B and
JAK/STAT pathways in RAW 264.7 macrophages
VL - 14
ID - 8894
ER -
TY - JOUR
AB - As a widely applied nanomaterial, silver nanomaterials (AgNMs) have increased
public concern about their potential adverse biological effects. However, there are
few related researches on the long-term toxicity, especially on the reversibility
of AgNMs in vivo. In the current study, this issue was tackled by exploring liver
damage after an intravenous injection of silver nanorods with golden cores
(Au@AgNRs) and its potential recovery in a relatively long term (8 w). After the
administration of Au@AgNRs into rats, Ag was found to be rapidly cleared from blood
within 10 min and mainly accumulated in liver as well as spleen until 8 w. All
detected parameters almost displayed a two-stage response to Au@AgNRs
administration, including biological markers, histological changes and metabolic
variations. For the short-term (2 w) responses, some toxicological parameters
(hematological changes, cytokines, liver damages etc.) significantly changed
compared to control and AuNRs group. However, after a 6-week recovery, all
abovementioned changes mostly returned to the normal levels in the Au@AgNRs group.
These indicated that after a lengthy period, acute bioeffects elicited by AgNMs
could be followed by the adaptive recovery, which will provide a novel and valuable
toxicity mechanism of AgNMs for potential biomedical applications of AgNMs.
AN - rayyan-553780882
AU - Liu, Y.
AU - Wen, H. R.
AU - Wu, X. C.
AU - Wu, M. Y.
AU - Liu, L.
AU - Wang, J. H.
AU - Huo, G. T.
AU - Lyu, J. J.
AU - Xie, L. M.
AU - Dan, M.
DO - 10.3390/nano11102656
IS - 10
KW - Rats
Liver
Tocopherols
PY - 2021
SN - 2079-4991
ST - The Bio-Persistence of Reversible Inflammatory, Histological Changes and
Metabolic Profile Alterations in Rat Livers after Silver/Gold Nanorod
Administration
T2 - NANOMATERIALS
TI - The Bio-Persistence of Reversible Inflammatory, Histological Changes and
Metabolic Profile Alterations in Rat Livers after Silver/Gold Nanorod
Administration
VL - 11
Y2 - 10
ID - 8895
ER -
TY - JOUR
AB - BACKGROUND: Neuroinflammation plays an important role in the onset and
progression of neurodegenerative diseases such as Alzheimer's disease.
Lipopolysaccharide (LPS, endotoxin) levels are higher in the brains of Alzheimer's
disease patients and are associated with neuroinflammation and cognitive decline,
while neural cholinergic signaling controls inflammation. This study aimed to
examine the efficacy of galantamine, a clinically approved cholinergic agent, in
alleviating LPS-induced neuroinflammation and cognitive decline as well as the
associated mechanism. METHODS: Mice were treated with galantamine (4 mg/kg,
intraperitoneal injection) for 14 days prior to LPS exposure
(intracerebroventricular injection). Cognitive tests were performed, including the
Morris water maze and step-through tests. mRNA expression of the microglial marker
(CD11b), astrocytic marker (GFAP), and pro-inflammatory cytokines (IL-1β, IL-6, and
TNF-α) were examined in the hippocampus by quantitative RT-PCR. The inflammatory
signaling molecule, nuclear factor-kappa B (NF-κB p65), and synapse-associated
proteins (synaptophysin, SYN, and postsynaptic density protein 95, PSD-95) were
examined in the hippocampus by western blotting. Furthermore, NF-κB p65 levels in
microglial cells and hippocampal neurons were examined in response to LPS and
galantamine. RESULTS: Galantamine treatment prevented LPS-induced deficits in
spatial learning and memory as well as memory acquisition of the passive avoidance
response. Galantamine decreased the expression of microglia and astrocyte markers
(CD11b and GFAP), pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α), and NF-κB
p65 in the hippocampus of LPS-exposed mice. Furthermore, galantamine ameliorated
LPS-induced loss of synapse-associated proteins (SYN and PSD-95) in the
hippocampus. In the in vitro study, LPS increased NF-κB p65 levels in microglia
(BV-2 cells); the supernatant of LPS-stimulated microglia (Mi-sup), but not LPS,
decreased the viability of hippocampal neuronal cells (HT-22 cells) and increased
NF-κB p65 levels as well as expression of pro-inflammatory cytokines (IL-1β, IL-6)
in HT-22 cells. Importantly, galantamine reduced the inflammatory response not only
in the BV-2 microglia cell line, but also in the HT-22 hippocampal neuronal cell
line. CONCLUSIONS: These findings indicate that galantamine could be a promising
treatment to improve endotoxin-induced cognitive decline and neuroinflammation in
neurodegenerative diseases.
AN - rayyan-553782279
AU - Liu, Y.
AU - Zhang, Y.
AU - Zheng, X.
AU - Fang, T.
AU - Yang, X.
AU - Luo, X.
AU - Guo, A.
AU - Newell, K. A.
AU - Huang, X. F.
AU - Yu, Y.
DO - 10.1186/s12974-018-1141-5
IS - 1
J2 - J Neuroinflammation
KW - Animals
*Cognition Disorders/chemically induced/drug therapy/pathology
Cytokines/genetics/metabolism
Galantamine/*therapeutic use
Hippocampus/*drug effects/ultrastructure
Inflammation/*chemically induced/drug therapy
Male
Maze Learning/drug effects
Memory/drug effects
Mice, Inbred Strains
Microglia/drug effects/metabolism
Nerve Tissue Proteins/genetics/metabolism
Neuronal Plasticity/*drug effects
Nootropic Agents/*therapeutic use
RNA, Messenger/metabolism
Silver Staining
Mice
Galantamine
Inflammation
Lipopolysaccharides
Cognition
LA - eng
N1 - Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogen
Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
cbpeliuyinew@163.com.; Jiangsu Key Laboratory of New Drug Research and Clinical
Pharmacy, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
cbpeliuyinew@163.com.; Illawarra Health and Medical Research Institute, School of
Medicine, University of Wollongong, Wollongong, NSW, 2522, Australia.
cbpeliuyinew@163.com.; Jiangsu Key Laboratory of New Drug Research and Clinical
Pharmacy, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.; Jiangsu Key
Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University,
Xuzhou, 221004, Jiangsu, China.; Jiangsu Key Laboratory of New Drug Research and
Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.;
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical
University, Xuzhou, 221004, Jiangsu, China.; Jiangsu Key Laboratory of New Drug
Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221004, Jiangsu,
China.; Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou
Medical University, Xuzhou, 221004, Jiangsu, China.; Illawarra Health and Medical
Research Institute, School of Medicine, University of Wollongong, Wollongong, NSW,
2522, Australia.; Jiangsu Key Laboratory of Immunity and Metabolism, Department of
Pathogen Biology and Immunology, Xuzhou Medical University, Xuzhou, 221004,
Jiangsu, China.; Illawarra Health and Medical Research Institute, School of
Medicine, University of Wollongong, Wollongong, NSW, 2522, Australia.; Jiangsu Key
Laboratory of Immunity and Metabolism, Department of Pathogen Biology and
Immunology, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.
yinghua@uow.edu.au.
PY - 2018
SP - 112
ST - Galantamine improves cognition, hippocampal inflammation, and synaptic
plasticity impairments induced by lipopolysaccharide in mice
T2 - Journal of neuroinflammation
TI - Galantamine improves cognition, hippocampal inflammation, and synaptic
plasticity impairments induced by lipopolysaccharide in mice
VL - 15
Y2 - 4 y3 - 18
ID - 10189
ER -
TY - JOUR
AB - A facile and general strategy for preparing uniform and multifunctional
polyphenol-based colloidal particles through amine-catalyzed polymerization-induced
self-assembly is described. The size and interfacial adhesion of polyphenol spheres
can be easily controlled over a wide range via adjusting the concentration of the
cosolvent and monomer. Moreover, the polyphenol spheres showed excellent thermal
and chemical stability and highly active properties and could efficiently deplete
the reactive oxygen species (ROS), which are helpful for in vivo ROS regulation for
inflammatory therapeutic. The accessible and versatile method provides a feasible
way for the rational engineering of multifunctional polyphenol spheres, which have
great potential in many fields. © 2021 American Chemical Society.
AN - rayyan-553780883
AU - Liu, Z.
AU - Yu, W.
AU - Sheng, W.
AU - Li, R.
AU - Guo, H.
AU - Feng, X.
AU - Li, Q.
AU - Wang, R.
AU - Li, W.
AU - Jia, X.
DO - 10.1021/acs.biomac.1c01158
IS - 1
KW - Amines
Catalysis
Polymerization
Polyphenols
Chemical stability
Self assembly
4 nitrophenol
amine
catechol
dextran sulfate
gold nanoparticle
mesalazine
monomer
phenol
phloroglucinol
poly(phloroglucinol)
polyphenol
pyrogallol
Schiff base
self assembled nanoparticle
silver nanoparticle
unclassified drug
Catalyzed polymerization
Colloidal particle
Controllable synthesis
Cosolvents
In-vivo
Interfacial adhesions
Property
Thermal and chemical stabilities
animal experiment
animal model
animal tissue
Article
controlled study
cross linking
cytotoxicity
drug stability
EC50
histology
hydrogen bond
male
Michael addition
mouse
nonhuman
polymerization
thermostability
ulcerative colitis
ultrasound assisted extraction
zeta potential
catalysis
Spheres
Polymers
Amination
PY - 2022
SP - 140-149
ST - Controllable Synthesis of Polyphenol Spheres via Amine-Catalyzed
Polymerization-Induced Self-Assembly
T2 - Biomacromolecules
TI - Controllable Synthesis of Polyphenol Spheres via Amine-Catalyzed
Polymerization-Induced Self-Assembly
85122008165&doi=10.1021%2facs.biomac.1c01158&partnerID=40&md5=709b6b94af362ab1b1e53
27e3eeb1c85
VL - 23
ID - 8896
ER -
TY - JOUR
AB - Background: With the continued integration of engineered nanomaterials (ENMs)
into everyday applications, it is important to understand their potential for
inducing adverse human health effects. However, standard in vitro hazard
characterisation approaches suffer limitations for evaluating ENM and so it is
imperative to determine these potential hazards under more physiologically relevant
and realistic exposure scenarios in target organ systems, to minimise the necessity
for in vivo testing. The aim of this study was to determine if acute (24 h) and
prolonged (120 h) exposures to five ENMs (TiO2, ZnO, Ag, BaSO4 and CeO2) would have
a significantly different toxicological outcome (cytotoxicity, (pro-)inflammatory
and genotoxic response) upon 3D human HepG2 liver spheroids. In addition, this
study evaluated whether a more realistic, prolonged fractionated and repeated ENM
dosing regime induces a significantly different toxicity outcome in liver spheroids
as compared to a single, bolus prolonged exposure. Results: Whilst it was found
that the five ENMs did not impede liver functionality (e.g. albumin and urea
production), induce cytotoxicity or an IL-8 (pro-)inflammatory response, all were
found to cause significant genotoxicity following acute exposure. Most
statistically significant genotoxic responses were not dose-dependent, with the
exception of TiO2. Interestingly, the DNA damage effects observed following acute
exposures, were not mirrored in the prolonged exposures, where only 0.2–5.0 µg/mL
of ZnO ENMs were found to elicit significant (p ≤ 0.05) genotoxicity. When
fractionated, repeated exposure regimes were performed with the test ENMs, no
significant (p ≥ 0.05) difference was observed when compared to the single, bolus
exposure regime. There was < 5.0% cytotoxicity observed across all exposures, and
the mean difference in IL-8 cytokine release and genotoxicity between exposure
regimes was 3.425 pg/mL and 0.181%, respectively. Conclusion: In conclusion, whilst
there was no difference between a single, bolus or fractionated, repeated ENM
prolonged exposure regimes upon the toxicological output of 3D HepG2 liver
spheroids, there was a difference between acute and prolonged exposures. This study
highlights the importance of evaluating more realistic ENM exposures, thereby
providing a future in vitro approach to better support ENM hazard assessment in a
routine and easily accessible manner.[Figure not available: see fulltext.] © 2021,
The Author(s).
AN - rayyan-553780885
AU - Llewellyn, S. V.
AU - Conway, G. E.
AU - Zanoni, I.
AU - Jørgensen, A. K.
AU - Shah, U. K.
AU - Seleci, D. A.
AU - Keller, J. G.
AU - Kim, J. W.
AU - Wohlleben, W.
AU - Jensen, K. A.
AU - Costa, A.
AU - Jenkins, G. J. S.
AU - Clift, M. J. D.
AU - Doak, S. H.
DO - 10.1186/s12951-021-00938-w
IS - 1
KW - Engineered nanomaterials
Genotoxicity
In vitro liver models
Nanotoxicology
Physiologically relevant exposure
Albumins
Cell Proliferation
Cytokines
DNA Damage
Hep G2 Cells
Humans
Liver
Mutagenicity Tests
Nanostructures
Particle Size
Urea
Barite
Barium sulfate
Cerium oxide
Cytotoxicity
Hazards
Silver compounds
Titanium dioxide
Zinc oxide
5,5 dimethyl 1 pyrroline 1 oxide
aflatoxin B1
albumin
alcohol
barium sulfate
bovine serum albumin
bromocresol green
carbon dioxide
carcinogen
cerium oxide
copper
edetic acid
glucose
glutamine
gold
helium
horseradish peroxidase
hydroxylamine
interleukin 6
interleukin 8
nanomaterial
nitric acid
nitrogen
nitrone
oxygen
phosphate buffered saline
polysorbate 20
potassium chloride
silicon
silver
streptavidin
streptomycin
superoxide
titanium dioxide
trypsin
urea
water
zinc oxide
albuminoid
cytokine
Genotoxic response
Hazard Assessment
Human health effects
Potential hazards
Realistic exposure scenario
Urea production
Article
cell culture
cell suspension
cytokinesis
cytotoxicity
DNA damage
drug exposure
electrophoretic mobility
fetal bovine serum
genotoxicity
Hep-G2 cell line
hydrophilicity
hydrophobicity
incubation time
liver
micronucleus test
nonhuman
particle size
physical chemistry
relative humidity
trypan blue assay
ultrasound
vacuum
wavelength dispersive X ray fluorescence spectroscopy
zeta potential
cell proliferation
chemical phenomena
drug effect
human
metabolism
mutagen testing
pathology
Oxide minerals
Humanities
Humanism
PY - 2021
ST - Understanding the impact of more realistic low-dose, prolonged engineered
nanomaterial exposure on genotoxicity using 3D models of the human liver
T2 - Journal of Nanobiotechnology
TI - Understanding the impact of more realistic low-dose, prolonged engineered
nanomaterial exposure on genotoxicity using 3D models of the human liver
85109028240&doi=10.1186%2fs12951-021-00938-
w&partnerID=40&md5=706c1738d37d6ec48fb74b817830a215
VL - 19
ID - 8898
ER -
TY - JOUR
AB - OBJECTIVE: To characterize the pathological features of right ventricular
dysplasia (RVD). DESIGN: Retrospective morphological case study. SETTING: Three
referral-based university medical centres. PATIENTS: Thirteen subjects (one female)
aged 16 to 55 years including 10 necropsy hearts from sudden deaths out of
hospital, one explant heart and two partial right ventricular resections from
patients with intractable ventricular tachycardia. MAIN RESULTS: Most hearts showed
hypertrophy and localized or generalized dilatation of the right ventricle.
Transillumination revealed myocardial thinning of variable configuration usually
conforming to regions of dilatation. Common sites of involvement were apex,
infundibular region and posterobasal wall. Histologically, focal or extensive
segments of right ventricular myocardium were absent or replaced. Three patterns
were found: right ventricle markedly thinned, epicardium and endocardium
contiguous, virtually no intervening tissue; wall normal thickness or thinned,
myocardium almost totally replaced by fat; and wall normal or thin, myocardium
largely replaced by fat with scattered residual myocardial cells and fibrous tissue
(the predominant pattern). Endocardial fibrosis was present in eight cases and
focal mononuclear cell infiltrates in 10. Electron microscopy in two cases showed
nonspecific findings. CONCLUSIONS: RVD has gross and microscopic features which
permit its recognition. While a majority of cases are likely congenital (genetic or
acquired in utero), the possibility of postnatally acquired conditions
(inflammatory, toxic, ischemic) inducing RVD must be explored. The incidence and
importance of RVD as a cause of sudden death can only be assessed by continued
systematic and detailed studies of patients with recurrent ventricular tachycardia
and of hearts, especially from sudden death victims. Although uncommon, RVD should
be considered in the differential diagnosis of arrhythmia and sudden death by both
clinicians and pathologists.
AN - rayyan-553782250
AU - Lobo, F. V.
AU - Heggtveit, H. A.
AU - Butany, J.
AU - Silver, M. D.
AU - Edwards, J. E.
IS - 3
J2 - Can J Cardiol
KW - Adolescent
Adult
Cardiomyopathies/pathology
Death, Sudden, Cardiac/*pathology
Dilatation, Pathologic
Endocardium/pathology
Endomyocardial Fibrosis/pathology
Female
Heart Defects, Congenital/*pathology
Heart Ventricles/*abnormalities/pathology
Humans
Male
Middle Aged
Myocardium/*pathology
Retrospective Studies
Tachycardia/pathology
LA - eng
N1 - Department of Pathology, Hamilton Civic Hospitals, Ontario.
PY - 1992
SN - 0828-282X (Print)
SP - 261-8
ST - Right ventricular dysplasia: morphological findings in 13 cases
T2 - The Canadian journal of cardiology
TI - Right ventricular dysplasia: morphological findings in 13 cases
VL - 8
Y2 - 4
ID - 10160
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly used in commercial and medical
applications. However, AgNPs may induce toxicity, extracellular matrix (ECM)
changes and inflammatory responses. Fibroblasts are key players in remodeling
processes and major producers of the ECM. The aims of this study were to explore
the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung
slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory
responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different
sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 µg/mL. Changes in
synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1.
Ag10 and Ag75 affected cell viability, with significantly reduced metabolic
activities at 10 µg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly
increased procollagen I synthesis and release of IL-8, prostaglandin E2, RANTES and
eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data
indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with
altered procollagen and proinflammatory cytokine secretion in fibroblasts over
time. Hence, careful characterizations of AgNPs are of importance, and future
studies should include timepoints beyond 24 h. © 2020 by the authors. Licensee
AN - rayyan-553780888
AU - Löfdahl, A.
AU - Jern, A.
AU - Flyman, S.
AU - Kåredal, M.
AU - Larsson-Callerfelt, A. K.
DO - 10.3390/nano10091868
IS - 9
KW - Cytokines
Extracellular matrix
Growth factors
Human lung fibroblasts
Precision-cut lung slices
Procollagen
Toxicity
Humanities
Humanism
Humans
Cell Survival
Lung
Nanoparticles
PY - 2020
SP - 1-12
ST - Silver nanoparticles alter cell viability ex vivo and in vitro and induce
proinflammatory effects in human lung fibroblasts
T2 - Nanomaterials
TI - Silver nanoparticles alter cell viability ex vivo and in vitro and induce
proinflammatory effects in human lung fibroblasts
85091518379&doi=10.3390%2fnano10091868&partnerID=40&md5=aa5b5c2033cd66266e2845a9ca4
ecf69
VL - 10
ID - 8901
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly used in commercial and medical
applications. However, AgNPs may induce toxicity, extracellular matrix (ECM)
changes and inflammatory responses. Fibroblasts are key players in remodeling
processes and major producers of the ECM. The aims of this study were to explore
the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung
slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory
responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different
sizes, 10 nm and 75 nm, at concentrations 2 mu g/mL and 10 mu g/mL. Changes in
synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1.
Ag10 and Ag75 affected cell viability, with significantly reduced metabolic
activities at 10 mu g/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly
increased procollagen I synthesis and release of IL-8, prostaglandin E-2, RANTES
and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our
data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro
with altered procollagen and proinflammatory cytokine secretion in fibroblasts over
time. Hence, careful characterizations of AgNPs are of importance, and future
studies should include timepoints beyond 24 h.
AN - rayyan-553780889
AU - Lofdahl, A.
AU - Jern, A.
AU - Flyman, S.
AU - Karedal, M.
AU - Larsson-Callerfelt, A. K.
DO - 10.3390/nano10091868
IS - 9
KW - Humanities
Humanism
Humans
Cell Survival
Lung
Nanoparticles
PY - 2020
SN - 2079-4991
ST - Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce
Proinflammatory Effects in Human Lung Fibroblasts
T2 - NANOMATERIALS
TI - Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce
Proinflammatory Effects in Human Lung Fibroblasts
VL - 10
Y2 - 9
ID - 8902
ER -
TY - JOUR
AB - It is unknown whether the flavonoid rutin can protect the silver catfish
liver in response to exposure to a known stressor, such as the prophylactic usage
of the antimicrobial agent oxytetracycline. Thus, the current study aimed to assess
the effect of rutin incorporation into the silver catfish diet formulation on
oxytetracycline-induced liver oxidative stress and apoptosis. Fish were split into
four groups as follows: control, rutin (1.5 g kg diet−1), oxytetracycline (0.1 g kg
diet−1) and rutin+oxytetracycline (1.5 g kg diet−1 and 0.1 g kg diet−1,
respectively). After two weeks of feeding with the different diets (standard,
rutin-, oxytetracycline and rutin+oxytetracycline-added diets), fish were
euthanized to collect the liver. Although the rutin-added diet was unable to
recover glutathione peroxidase activity, ascorbic acid and reduced glutathione
(GSH) levels, which were depleted due to oxytetracycline consumption, it markedly
diminished the oxidized glutathione (GSSG) content, thus decreasing the GSSG to GSH
ratio, an important index of oxidative stress. It also increased glutathione
reductase and markedly augmented glucose-6-phosphate dehydrogenase activities,
which were declined after oxytetracycline ingestion. Furthermore, the rutin-added
diet reestablished superoxide dismutase and catalase activities and reduced lipid
peroxidation, nitric oxide and superoxide anion levels as well, all changes
resulting from oxytetracycline consumption. Finally, it also prevented
oxytetracycline-induced apoptosis through increasing heat shock protein 70 and
markedly decreasing high mobility group box 1 and, consequently, reducing cleaved
caspase-3 protein levels. Therefore, in conclusion, the incorporation of this
flavonoid to the silver catfish diet protected the liver against oxytetracycline-
induced liver oxidative stress and apoptosis. © 2020 Elsevier Inc.
AN - rayyan-553780892
AU - Londero, É P.
AU - Bressan, C. A.
AU - Pês, T. S.
AU - Saccol, E. M. H.
AU - Finamor, I. A.
DO - 10.1016/j.cbpc.2020.108848
KW - Antimicrobial
Fish
Flavonoid
Prophylactic
Stressor
Animal Feed
Animals
Antioxidants
Apoptosis
Biomarkers
Catfishes
Liver
Oxidative Stress
Oxytetracycline
Rutin
ascorbic acid
caspase 3
catalase
glucose 6 phosphate dehydrogenase
glutathione
glutathione disulfide
lipid hydroperoxide
nitric oxide
oxytetracycline
rutoside
superoxide
thiobarbituric acid reactive substance
antiinfective agent
antioxidant
biological marker
animal experiment
animal tissue
apoptosis
Article
catfish
controlled study
lipid peroxidation
liver protection
nonhuman
oxidative stress
priority journal
protein cleavage
tissue homogenate
animal
animal food
drug effect
liver
metabolism
pathology
PY - 2021
ST - Rutin-added diet protects silver catfish liver against oxytetracycline-
induced oxidative stress and apoptosis
T2 - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
TI - Rutin-added diet protects silver catfish liver against oxytetracycline-
induced oxidative stress and apoptosis
85089953226&doi=10.1016%2fj.cbpc.2020.108848&partnerID=40&md5=35ce70f4612598a47e6b4
79ba82545fb
VL - 239
ID - 8905
ER -
TY - JOUR
AB - Concerns about the bioaccumulation and toxicity of gold nanoparticles inside
humans have recently risen. HT-29 and HepG2 cell lines and Wistar rats were exposed
to 10, 30 or 60 nm gold nanoparticles to determine their tissue distribution,
subcellular location and deleterious effects. Cell viability, ROS production and
DNA damage were evaluated in vitro. Lipid peroxidation and protein carbonylation
were determined in liver. ICP-MS measurements showed the presence of gold in
intestine, kidney, liver, spleen, feces and urine. Subcellular locations of gold
nanoparticles were observed in colon cells and liver samples by transmission
electron microscopy. Inflammatory markers in liver and biochemical parameters in
plasma were measured to assess the inflammatory status and presence of tissue
damage. The size of the nanoparticles determined differences in the biodistribution
and the excretion route. The smallest nanoparticles showed more deleterious
effects, confirmed by their location inside the cell nucleus and the higher DNA
damage. (c) 2017 Elsevier Inc. All rights reserved.
AN - rayyan-553780893
AU - Lopez-Chaves, C.
AU - Soto-Alvaredo, J.
AU - Montes-Bayon, M.
AU - Bettmer, J.
AU - Llopis, J.
AU - Sanchez-Gonzalez, C.
DO - 10.1016/j.nano.2017.08.011
IS - 1
PY - 2018
SN - 1549-9634 1549-9642
SP - 1-12
ST - Gold nanoparticles: Distribution, bioaccumulation and toxicity. In vitro and
in vivo studies
TI - Gold nanoparticles: Distribution, bioaccumulation and toxicity. In vitro and
in vivo studies
VL - 14
Y2 - 1
ID - 8906
ER -
TY - JOUR
AB - Background: Animal models remain at that time a reference tool to predict
potential pulmonary adverse effects of nanomaterials in humans. However, in a
context of reduction of the number of animals used in experimentation, there is a
need for reliable alternatives. In vitro models using lung cells represent relevant
alternatives to assess potential nanomaterial acute toxicity by inhalation,
particularly since advanced in vitro methods and models have been developed.
Nevertheless, the ability of in vitro experiments to replace animal experimentation
for predicting potential acute pulmonary toxicity in human still needs to be
carefully assessed. The aim of the study was to evaluate the differences existing
between the in vivo and the in vitro approaches for the prediction of nanomaterial
toxicity and to find advanced methods to enhance in vitro predictivity. For this
purpose, rats or pneumocytes in co-culture with macrophages were exposed to the
same poorly soluble and poorly toxic TiO2 and CeO2 nanomaterials, by the
respiratory route in vivo or using more or less advanced methodologies in vitro.
After 24 h of exposure, biological responses were assessed focusing on pro-
inflammatory effects and quantitative comparisons were performed between the in
vivo and in vitro methods, using compatible dose metrics. Results: For each dose
metric used (mass/alveolar surface or mass/macrophage), we observed that the most
realistic in vitro exposure method, the air-liquid interface method, was the most
predictive of in vivo effects regarding biological activation levels. We also noted
less differences between in vivo and in vitro results when doses were normalized by
the number of macrophages rather than by the alveolar surface. Lastly, although we
observed similarities in the nanomaterial ranking using in vivo and in vitro
approaches, the quality of the data-set was insufficient to provide clear ranking
comparisons. Conclusions: We showed that advanced methods could be used to enhance
in vitro experiments ability to predict potential acute pulmonary toxicity in vivo.
Moreover, we showed that the timing of the dose delivery could be controlled to
enhance the predictivity. Further studies should be necessary to assess if air-
liquid interface provide more reliable ranking of nanomaterials than submerged
methods.
AN - rayyan-553780894
AU - Loret, T.
AU - Rogerieux, F.
AU - Trouiller, B.
AU - Braun, A.
AU - Egles, C.
AU - Lacroix, G.
DO - 10.1186/s12989-018-0260-6
PY - 2018
SN - 1743-8977
ST - Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly
soluble nanomaterials by using advanced in vitro methods
TI - Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly
soluble nanomaterials by using advanced in vitro methods
VL - 15
Y2 - 6 y3 - 4
ID - 8907
ER -
TY - JOUR
AB - Background: Sepsis is a syndrome of physiological, pathological and
biochemical abnormalities caused by infection. Although the mortality rate is lower
than before, many survivors have persistent infection, which means sepsis calls for
new treatment. After infection, inflammatory mediators were largely released into
the blood, leading to multiple organ dysfunction. Therefore, anti-infection and
anti-inflammation are critical issues in sepsis management. Results: Here, we
successfully constructed a novel nanometer drug loading system for sepsis
management, FZ/MER-AgMOF@Bm. The nanoparticles were modified with LPS-treated bone
marrow mesenchymal stem cell (BMSC) membrane, and silver metal organic framework
(AgMOF) was used as the nanocore for loading FPS-ZM1 and meropenem which was
delivery to the infectious microenvironments (IMEs) to exert dual anti-inflammatory
and antibacterial effects. FZ/MER-AgMOF@Bm effectively alleviated excessive
inflammatory response and eliminated bacteria. FZ/MER-AgMOF@Bm also played an anti-
inflammatory role by promoting the polarization of macrophages to M2. When sepsis
induced by cecal ligation and puncture (CLP) challenged mice was treated, FZ/MER-
AgMOF@Bm could not only reduce the levels of pro-inflammatory factors and lung
injury, but also help to improve hypothermia caused by septic shock and prolong
survival time. Conclusions: Together, the nanoparticles played a role in combined
anti-inflammatory and antimicrobial properties, alleviating cytokine storm and
protecting vital organ functions, could be a potential new strategy for sepsis
management. © 2023, The Author(s).
AN - rayyan-553780898
AU - Lu, L.
AU - Quan, L.
AU - Li, J.
AU - Yuan, J.
AU - Nie, X.
AU - Huang, X.
AU - Dong, H.
AU - Su, Y.
AU - Huang, Y.
AU - Kou, Q.
AU - Liu, L.
AU - Liu, H.
AU - Zhou, X.
AU - Gui, R.
AU - Gu, L.
DO - 10.1186/s12951-023-01913-3
IS - 1
KW - Antibacterial
Cytokine storm
Metal-organic framework
sepsis
Animals
Cell Membrane
Macrophages
Mice
Nanoparticles
Sepsis
Bone
Cell culture
Mammals
Metal nanoparticles
Microorganisms
Organometallics
Stem cells
lipopolysaccharide
meropenem
nanocarrier
silver
silver metal organic framework
unclassified drug
antiinfective agent
nanoparticle
Anti-inflammatory and antimicrobial properties
Antibacterials
Cytokines
Functionalized nanoparticles
Metalorganic frameworks (MOFs)
Mortality rate
Stem-cell
animal cell
animal experiment
animal model
animal tissue
Article
bacterium
biocompatibility
bioengineering
bone marrow mesenchymal stem cell
cecal ligation and puncture-induced sepsis
cell membrane
controlled study
cytokine storm
disease management
drug dosage form comparison
drug loading system
drug safety
hypothermia
in vitro study
in vivo study
infection
inflammation
lung injury
M2 macrophage
macrophage
microenvironment
mouse
nonhuman
polarization
septic shock
survival time
animal
disease model
metabolism
Storms
Stem Cells
PY - 2023
ST - Bioengineered stem cell membrane functionalized nanoparticles combine anti-
inflammatory and antimicrobial properties for sepsis treatment
TI - Bioengineered stem cell membrane functionalized nanoparticles combine anti-
inflammatory and antimicrobial properties for sepsis treatment
85160377269&doi=10.1186%2fs12951-023-01913-
3&partnerID=40&md5=08b59414091e30f35696125a4e73191f
VL - 21
ID - 8911
ER -
TY - JOUR
AB - In recent years, the immune-potentiating effects of some widely used
chemotherapeutic agents have been increasingly appreciated. This provides a
rationale for combining conventional chemotherapy with immunotherapy strategies to
achieve durable therapeutic benefits. Previous studies have implicated the
immunomodulatory effects of melphalan, an alkylating agent commonly used to treat
multiple myeloma, but the underlying mechanisms remain obscure. In the present
study, we investigated the impact of melphalan on endogenous immune cells as well
as adoptively transferred tumor-specific CD4+ T cells in tumor-bearing mice. We
showed that melphalan treatment resulted in a rapid burst of inflammatory cytokines
and chemokines during the cellular recovery phase after melphalan-induced
myelodepletion and leukodepletion. After melphalan treatment, tumor cells exhibited
characteristics of immunogenic cell death, including membrane translocation of the
endoplasmic reticulum-resident calreticulin and extracellular release of high-
mobility group box 1. Additionally, there was enhanced tumor Ag uptake by dendritic
cells in the tumor-draining lymph node. Consistent with these immunomodulatory
effects, melphalan treatment of tumor-bearing mice led to the activation of the
endogenous CD8+ T cells and, more importantly, effectively drove the clonal
expansion and effector differentiation of adoptively transferred tumor-specific
CD4+ T cells. Notably, the combination of melphalan and CD4+ T cell adoptive cell
therapy was more efficacious than either treatment alone in prolonging the survival
of mice with advanced B cell lymphomas or colorectal tumors. These findings provide
mechanistic insights into melphalan's immunostimulatory effects and demonstrate the
therapeutic potential of combining melphalan with adoptive cell therapy utilizing
antitumor CD4+ T cells. Copyright © 2015 by The American Association of
Immunologists, Inc.
AN - rayyan-553780900
AU - Lu, X.
AU - Ding, Z. C.
AU - Cao, Y.
AU - Liu, C.
AU - Habtetsion, T.
AU - Yu, M.
AU - Lemos, H.
AU - Salman, H.
AU - Xu, H.
AU - Mellor, A. L.
AU - Zhou, G.
DO - 10.4049/jimmunol.1401894
IS - 4
KW - Animals
Antineoplastic Agents, Alkylating
Blotting, Western
CD4-Positive T-Lymphocytes
Combined Modality Therapy
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunotherapy, Adoptive
Melphalan
Mice
Mice, Inbred BALB C
Mice, Transgenic
Neoplasms, Experimental
calreticulin
CXCL11 chemokine
gamma interferon
interleukin 10
interleukin 18
interleukin 1beta
interleukin 22
interleukin 27
interleukin 5
melphalan
silver
alkylating agent
adoptive immunotherapy
adoptive transfer
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
B cell lymphoma
bone marrow depression
CD4+ T lymphocyte
CD8+ T lymphocyte
cell expansion
clonal variation
colorectal carcinoma
controlled study
cytokine release
dendritic cell
drug mechanism
immunogenicity
immunomodulation
leukopenia
mouse
nonhuman
protein localization
animal
Bagg albino mouse
flow cytometry
multimodality cancer therapy
procedures
transgenic mouse
transplantation
Western blotting
Immunotherapy
PY - 2015
SP - 2011-2021
ST - Alkylating agent melphalan augments the efficacy of adoptive immunotherapy
using tumor-specific CD4+ T cells
T2 - Journal of Immunology
TI - Alkylating agent melphalan augments the efficacy of adoptive immunotherapy
using tumor-specific CD4+ T cells
84922552740&doi=10.4049%2fjimmunol.1401894&partnerID=40&md5=9f293dc305ce793e08fc132
b0b2044fe
VL - 194
ID - 8913
ER -
TY - JOUR
AB - AA amyloidosis is a systemic disease that develops secondary to chronic
inflammatory diseases Macrophages are often found in the vicinity of amyloid
deposits and considered to play a role in both formation and degradation of amyloid
fibrils. In spleen reside at least three types of macrophages, red pulp macrophages
(RPM), marginal zone macrophages (MZM), metallophilic marginal zone macrophages
(MMZM). MMZM and MZM are located in the marginal zone and express a unique
collection of scavenger receptors that are involved in the uptake of blood-born
particles. The murine AA amyloid model that resembles the human form of the disease
has been used to study amyloid effects on different macrophage populations. Amyloid
was induced by intravenous injection of amyloid enhancing factor and subcutaneous
injections of silver nitrate and macrophages were identified with specific
antibodies. We show that MZMs are highly sensitive to amyloid and decrease in
number progressively with increasing amyloid load. Total area of MMZMs is
unaffected by amyloid but cells are activated and migrate into the white pulp. In a
group of mice spleen macrophages were depleted by an intravenous injection of
clodronate filled liposomes. Subsequent injections of AEF and silver nitrate showed
a sustained amyloid development. RPMs that constitute the majority of macrophages
in spleen, appear insensitive to amyloid and do not participate in amyloid
formation. © 2013 Lundmark et al.
AN - rayyan-553780901
AU - Lundmark, K.
AU - Shariatpanahi, A. V.
AU - Westermark, G. T.
DO - 10.1371/journal.pone.0079104
IS - 11
KW - Amyloidosis
Animals
Animals, Outbred Strains
Female
Humans
Macrophages
Mice
Spleen
amyloid enhancing factor
clodronic acid
enzyme
liposome
silver nitrate
unclassified drug
amyloidosis
animal cell
animal experiment
animal model
animal tissue
article
cell population
controlled study
cytotoxicity
drug mechanism
female
macrophage
macrophage function
marginal zone macrophage
metallophilic marginal zone macrophage
mouse
nonhuman
red pulp macrophage
regulatory mechanism
sensitivity analysis
spleen cell
Amyloid
PY - 2013
ST - Depletion of spleen macrophages delays AA amyloid development: A study
performed in the rapid mouse model of aa amyloidosis
T2 - PLoS ONE
TI - Depletion of spleen macrophages delays AA amyloid development: A study
performed in the rapid mouse model of aa amyloidosis
84893374758&doi=10.1371%2fjournal.pone.0079104&partnerID=40&md5=510bc74a9be9fad68c3
f534682fdf946
VL - 8
ID - 8914
ER -
TY - JOUR
AB - Hyaluronic acid (HA) is increasingly used for a number of medical device
applications. Since the chemical structure of HA is identical no matter its
bacterial or animal origin, it should be the ideal biomaterial. However, short term
transient inflammatory reactions are common, while rare long-term adverse events
may correlate with subclinical chronic inflammation. Concern has been raised that
low molecular weight components or degradation fragments from implanted HA may
directly stimulate inflammatory reactions. This study examined a panel of HA
molecular weights from the unitary disaccharide up to 1.7 x 10(6) Dalton lengths,
in which endotoxin was assayed at a very low level (less than 0.03 EU/mg). The
murine cell line RAW 264.7, rat splenocytes, and rat adherent differentiated
primary macrophages were assayed for nitric oxide production under a variety of
inflammatory conditions plus or minus HA. Under the highest inflammatory states,
nitric oxide production was mildly suppressed by HMW-HA while slightly augmented by
LMW-HA at mg/mL concentrations. However, at micromolar concentrations fragments
below 5000 Daltons, thought to have drug-like qualities, were without effect. These
data support the hypothesis that if endotoxin is reduced to an extremely low level,
LMW-HA may not directly provoke normal tissue macrophage-mediated inflammatory
reactions.
AN - rayyan-553782175
AU - Lyle, D. B.
AU - Breger, J. C.
AU - Baeva, L. F.
AU - Durfor, C. N.
AU - Wang, N. S.
AU - Langone, J. J.
DO - 10.1002/jbm.a.32760
IS - 3
J2 - J Biomed Mater Res A
KW - Animals
Biocompatible Materials/chemistry/pharmacology
Cell Line
Humans
Hyaluronic Acid/*chemistry/immunology/*pharmacology
Interferon-gamma/immunology
Lipopolysaccharides/immunology
Macrophages/cytology/*drug effects/*metabolism
Materials Testing
Mice
Molecular Weight
Nitric Oxide/*biosynthesis
Rats
Spleen/cytology
Macrophages
Nitric Oxide
LA - eng
N1 - Center for Devices and Radiological Health, Office of Science and Engineering
Laboratories, FDA, Silver Spring, Maryland 20993-002, USA.
PY - 2010
SP - 893-904
ST - Low molecular weight hyaluronic acid effects on murine macrophage nitric
oxide production
T2 - Journal of biomedical materials research. Part A
TI - Low molecular weight hyaluronic acid effects on murine macrophage nitric
oxide production
VL - 94
Y2 - 9 y3 - 1
ID - 10086
ER -
TY - JOUR
AB - Inflammatory reactions to biomaterials may include macrophage-mediated
generation of nitric oxide (NO), which may harm patient tissue or potentially
interfere with proper function of an implanted device. RAW 264.7 cells were grown
in culture and treated at various times with lipopolysaccharide (LPS, endotoxin),
murine recombinant gamma-interferon (mrIFN-gamma), and different preparations of
hyaluronic acid (HA). Increase in fluorescence of 2,3-diaminonaphthalene (DAN)
allowed for detection of initial (24 h or less) NO inflammatory responses of RAW
264.7 to LPS from E. coli O26:B6. By looking at early time points, mrIFN-gamma
augmentation of the LPS effect was observed, simulating a complex immune reaction.
Activation through nuclear factor-kappaB (NF-kappaB), was confirmed in this system
by parthenolide inhibition of LPS stimulation. Stimulation of RAW 264.7 by
different HA preparations resulted in NO responses that correlated with the amount
of LPS present. In the presence of mrIFN-gamma, a significant inflammatory reaction
to HA was observed when the concentration of contaminating LPS was as low as 0.15
EU/mL. NO production in the presence of mrIFN-gamma by RAW 264.7 may serve as a
convenient in vitro system to routinely screen biomaterials for potentially harmful
macrophage-mediated inflammation whereby the safety of implanted medical devices
might be compromised.
AN - rayyan-553782232
AU - Lyle, D. B.
AU - Durfor, C. N.
AU - Breger, J. C.
AU - Langone, J. J.
DO - 10.1002/jbm.a.32060
IS - 1
KW - 2-Naphthylamine/analogs & derivatives/pharmacology
Animals
Anti-Inflammatory Agents, Non-Steroidal/pharmacology
Biocompatible Materials/*adverse effects/pharmacology
Cell Line
Humans
Hyaluronic Acid/pharmacology
Inflammation/*immunology
Interferon-gamma/immunology/pharmacology
Lipopolysaccharides/immunology/pharmacology
Macrophages/cytology/drug effects/immunology
Materials Testing
Mice
Nitric Oxide/*immunology
Nitrites/metabolism
Sesquiterpenes/pharmacology
Inflammation
Nitric Oxide
Mass Screening
LA - eng
N1 - Division of Biology, Office of Science and Engineering Laboratories, Center
for Devices and Radiological Health, FDA,10903 New Hampshire Ave, Silver Spring,
Maryland 20993-0002, USA. dan.lyle@fda.hhs.gov
PY - 2009
SP - 82-93
ST - Screening biomaterials for stimulation of nitric oxide-mediated inflammation
TI - Screening biomaterials for stimulation of nitric oxide-mediated inflammation
VL - 90
Y2 - 7
ID - 10142
ER -
TY - JOUR
AB - Chronic inflammation may compromise function of implanted encapsulated
islets. Increased purity of alginate used for encapsulation prolongs encapsulated
graft function, correlating with decreased presence of impurities like bacterial
endotoxin. Limits for endotoxin contamination in biomaterials based on indirect
inhibition of function of embedded cells have yet to be established. In a coculture
system with RAW 264.7 monocyte/macrophage cells in the presence of 50 ng/mL murine
recombinant gamma-interferon (mrIFN-gamma), the insulin response to glucose
challenge of both rat and pig unencapsulated islets was prevented by endotoxin
(LPS) in the medium down to 0.3 EU/mL (LOEL), but not 0.06 EU/mL (NOEL). Evaluation
of nitrite concentrations in supernatants revealed that pig islets were more
resistant to LPS-stimulated macrophage mediators than rat islets. Encapsulation in
highly purified alginate produced little change in observed inhibitory effects of
macrophage-generated nitric oxide (NO) toward islet function. Chemically released
NO was much less effective in inhibiting insulin responsiveness to glucose
challenge than was coculture of islets with LPS and mrIFN-gamma-stimulated RAW
264.7. These results taken together with other data suggest that an upper limit of
0.3 EU/mL LPS within the encapsulating alginate will not impair the function of
implanted encapsulated islets by toxic concentrations of macrophage-mediated
inflammatory agents.
AN - rayyan-553782318
AU - Lyle, D. B.
AU - Langone, J. J.
DO - 10.1002/jbm.a.32351
IS - 4
KW - Animals
Endotoxins/*pharmacology
Glucose/pharmacology
Inflammation Mediators/*pharmacology
Insulin/*biosynthesis
Interferon-gamma/pharmacology
Islets of Langerhans/*drug effects/*metabolism
Macrophages/*drug effects/*metabolism
Mice
Nitroso Compounds/pharmacology
Rats
Sus scrofa
Macrophages
Insulin Resistance
LA - eng
N1 - Center for Devices and Radiological Health, Office of Science and Engineering
Laboratories, FDA, 10903 New Hampshire Ave, Silver Spring, Maryland 20993-002, USA.
dan.lyle@fda.hhs.gov
PY - 2009
SP - 1221-38
ST - Sensitivity of insulin production from encapsulated islets to endotoxin-
stimulated macrophage inflammatory mediators
TI - Sensitivity of insulin production from encapsulated islets to endotoxin-
stimulated macrophage inflammatory mediators
VL - 91
Y2 - 12 y3 - 15
ID - 10226
ER -
TY - JOUR
AB - Mast cells (MCs) play critical roles in allergic reactions and modulating the
activation of MCs could be an effective strategy to treat allergic diseases, which
cause a rapidly increasing threat to the public health. Herein, we described that
Magnolin, a major component from Flos magnoliae could inhibit IgE-dependent MCs
activation. We found Magnolin inhibited IgE/Ag-induced calcium mobilization,
degranulation, and cytokines release in LAD2 cells. Magnolin was also found to
attenuate IgE/Ag-induced mice paw swelling in a dose-dependent manner. Further
mechanistic studies suggested a possible anti-allergic and anti-inflammatory
effects of Magnolin in IgE/Ag-induced anaphylactic reactions. Thereby, Magnolin
could be a potential therapeutic agent for preventing mast cell-related immediate
and delayed allergic diseases. © 2019 Elsevier B.V.
AN - rayyan-553780905
AU - Ma, P.
AU - Che, D.
AU - Zhao, T.
AU - Zhang, Y.
AU - Li, C.
AU - An, H.
AU - Zhang, T.
AU - He, H.
DO - 10.1016/j.intimp.2019.105867
KW - Anti-anaphylactic
Calcium activation
Degranulation
Magnolin
Mast cells
Anaphylaxis
Animals
Anti-Allergic Agents
Antigens
Calcium
Cell Line
Cell Survival
Cytokines
Edema
Histamine
Humans
Hypersensitivity
Immunoglobulin E
Lignans
Male
Mast Cells
Mice, Inbred BALB C
Mitogen-Activated Protein Kinases
Peptide Hydrolases
antiallergic agent
immunoglobulin E
magnolin
silver
unclassified drug
antigen
calcium
cytokine
histamine
lignan
peptide hydrolase
adult
allergy
anaphylaxis
animal experiment
animal model
Article
calcium mobilization
cytokine release
cytotoxicity
degranulation
downstream processing
in vitro study
in vivo study
LAD 2 cell line
male
mouse
nonhuman
paw edema
priority journal
signal transduction
animal
Bagg albino mouse
cell line
cell survival
drug effect
edema
human
hypersensitivity
immunology
mast cell
metabolism
PY - 2019
ST - Magnolin inhibits IgE/Ag-induced allergy in vivo and in vitro
TI - Magnolin inhibits IgE/Ag-induced allergy in vivo and in vitro
85071974644&doi=10.1016%2fj.intimp.2019.105867&partnerID=40&md5=955207d76bae2678a3d
73784187f98ad
VL - 76
ID - 8918
ER -
TY - JOUR
AB - In this study, different phenolic extracts were obtained from the jaboticaba
skin meal (JSM), whose phenolic compounds were characterized and their
antibacterial activities were assessed. Moreover, the activity of lyophilized
ethanolic extract of jaboticaba skin (EEJS) on wound healing was analyzed in rats.
The JSM phenolic extracts were obtained in four ways: aqueous, methanolic,
ethanolic, and acetone extracts. The phenolic compounds were characterized in these
extracts by high-performance liquid chromatography, and their antibacterial
activities were evaluated. The in vivo experiment was divided into four groups and
received the following treatments: G1—silver sulfadiazine (positive control); G2—
EEJS at 10%; G3—EEJS at 5%, and G4—EEJS at 2.5%. The aqueous extract did not
inhibit the growing of any bacterium. The ethanolic, acetone, and methanolic
extracts inhibited the growing of all bacteria tested at the concentrations of
1.25%, 2.50%, and 5.00%, respectively. The ethanolic extract was the one that
showed the highest bacterial inhibition potential and the highest contents of
phenolic compounds, especially of catechin, epicatechin gallate, and epicatechin.
The G3 and G4 treatments presented faster wound healing compared to the G1 one, as
it promoted a less intense inflammatory reaction and full closure of the wounds at
an accelerated rate. © 2018 John Wiley & Sons A/S
AN - rayyan-553780906
AU - Machado, G. H. A.
AU - Marques, T. R.
AU - de Carvalho, T. C. L.
AU - Duarte, A. C.
AU - de Oliveira, F. C.
AU - Gonçalves, M. C.
AU - Piccoli, R. H.
AU - Corrêa, A. D.
DO - 10.1111/cbdd.13198
IS - 1
KW - antimicrobial
HPLC
Plinia jaboticaba
wound healing
Animals
Anthocyanins
Fruit
Male
Myrtaceae
Phenols
Plant Extracts
Rats
Rats, Wistar
Skin
Wound Healing
acetone
alcohol
catechin
coumaric acid
cyanidin chloride
delphinidin
epicatechin
epicatechin gallate
ferulic acid
flavonoid
gallic acid
jaboticaba skin meal extract
malvidin chloride
methanol
para coumaric acid
phenol derivative
plant extract
sulfadimethoxine
syringic acid
tannin derivative
unclassified drug
anthocyanin
antiinfective agent
animal cell
animal experiment
animal model
animal tissue
Article
bioassay
chromatography
cytotoxicity test
erythrocyte membrane
flow rate
fruit
hemolysis
histology
in vitro study
in vivo study
nonhuman
priority journal
rat
retention time
secondary infection
skin injury
Wistar rat
wound closure
animal
chemistry
drug effect
male
metabolism
pathology
skin
Phenol
PY - 2018
SP - 1333-1343
ST - Antibacterial activity and in vivo wound healing potential of phenolic
extracts from jaboticaba skin
T2 - Chemical Biology and Drug Design
TI - Antibacterial activity and in vivo wound healing potential of phenolic
extracts from jaboticaba skin
85049054431&doi=10.1111%2fcbdd.13198&partnerID=40&md5=c444c1d4ffcc36a88cc4768350106
1ac
VL - 92
ID - 8919
ER -
TY - JOUR
AB - Alzheimer's disease (AD) is multifactorial with unclear etiopathology. Due to
the complexity of AD, many attempted single therapy treatments, like Aβ
immunization, have generally failed. Therefore, there is a need for drugs with
multiple benefits. Naturally occurring phytochemicals with neuroprotective, anti-
amyloidogenic, antioxidative, and anti-inflammatory properties could be a possible
way out. In this study, the effect of Moringa oleifera (MO), a naturally occurring
plant with high antioxidative, anti-inflammatory, and neuroprotective effects, was
evaluated on hyperhomocysteinemia (HHcy) induced AD-like pathology in rats.
Homocysteine (Hcy) injection for 14 days was used to induce AD-like pathology.
Simultaneous MO extract gavage followed the injection as a preventive treatment or,
after injection completion, MO gavage was performed for another 14 days as a
curative treatment. MO was found to not only prevent but also rescue the oxidative
stress and cognitive impairments induced by Hcy treatment. Moreover, MO recovered
the decreased synaptic proteins PSD93, PSD95, Synapsin 1 and Synaptophysin, and
improved neurodegeneration. Interestingly, MO decreased the Hyc-induced tau
hyperphosphorylation at different sites including S-199, T-231, S-396, and S-404,
and at the same time decreased Aβ production through downregulation of BACE1. These
effects in HHcy rats were accompanied by a decrease in calpain activity under MO
treatment, supporting that calpain activation might be involved in AD pathogenesis
in HHcy rats. Taken together, our data, for the first time, provided evidence that
MO alleviates tau hyperphosphorylation and Aβ pathology in a HHcy AD rat model.
This and previous other studies support MO as a good candidate for, and could
provide new insights into, the treatment of AD and other tauopathies.
AN - rayyan-553782258
AU - Mahaman, Y. A. R.
AU - Huang, F.
AU - Wu, M.
AU - Wang, Y.
AU - Wei, Z.
AU - Bao, J.
AU - Salissou, M. T. M.
AU - Ke, D.
AU - Wang, Q.
AU - Liu, R.
AU - Wang, J. Z.
AU - Zhang, B.
AU - Chen, D.
AU - Wang, X.
DO - 10.3233/JAD-180091
IS - 3
J2 - J Alzheimers Dis
KW - Alzheimer Disease/chemically induced/*drug therapy/*pathology
Amyloid Precursor Protein Secretases/metabolism
Amyloid beta-Peptides/metabolism
Amyloid beta-Protein Precursor/metabolism
Animals
Aspartic Acid Endopeptidases/metabolism
Brain/drug effects/metabolism
*Cognition Disorders/chemically induced/drug therapy
Homocysteine/*toxicity
Male
Maze Learning/drug effects
*Moringa oleifera
Nerve Tissue Proteins/metabolism
Neurons/pathology/ultrastructure
Peptide Fragments/metabolism
Phosphorylation/drug effects
Plant Extracts/*therapeutic use
Rats
Silver Staining
tau Proteins/metabolism
Alzheimer Disease
Homocysteine
Moringa
Moringa oleifera
LA - eng
N1 - Department of Pathophysiology, School of Basic Medicine, Key Laboratory of
Education Ministry of China for Neurological Disorders, Tongji Medical College,
Huazhong University of Science and Technology, Wuhan, China.; Department of
Pathophysiology, School of Basic Medicine, Key Laboratory of Education Ministry of
China for Neurological Disorders, Tongji Medical College, Huazhong University of
Science and Technology, Wuhan, China.; Department of Pathophysiology, School of
Basic Medicine, Key Laboratory of Education Ministry of China for Neurological
Disorders, Tongji Medical College, Huazhong University of Science and Technology,
Wuhan, China.; Department of Pathophysiology, School of Basic Medicine, Key
Laboratory of Education Ministry of China for Neurological Disorders, Tongji
Medical College, Huazhong University of Science and Technology, Wuhan, China.;
Department of Pathophysiology, School of Basic Medicine, Key Laboratory of
Wuhan, China.; Department of Pathophysiology, School of Basic Medicine, Key
Laboratory of Education Ministry of China for Neurological Disorders, Tongji
Medical College, Huazhong University of Science and Technology, Wuhan, China.;
Department of Pathophysiology, School of Basic Medicine, Key Laboratory of
Wuhan, China.; Co-innovation Center of Neuroregeneration, Nantong University,
Nantong, China.; Department of Genetics and Genomic Sciences, Icahn School of
Medicine at Mount Sinai, New York, NY, USA.; School of Public Health, Wuhan
University of Science and Technology, Wuhan, China.; Department of Pathophysiology,
School of Basic Medicine, Key Laboratory of Education Ministry of China for
Neurological Disorders, Tongji Medical College, Huazhong University of Science and
Technology, Wuhan, China.; Co-innovation Center of Neuroregeneration, Nantong
University, Nantong, China.
PY - 2018
SP - 1141-1159
ST - Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like
Pathology and Cognitive Impairments
T2 - Journal of Alzheimer's disease : JAD
TI - Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like
Pathology and Cognitive Impairments
VL - 63
ID - 10168
ER -
TY - JOUR
AB - Background: Hepatotoxicity was one of the major side effects associated with
doxorubicin treatment in cancer chemotherapy. The synthesized silver nanoparticles
(AgNPs) from natural products such as algae especially green algae is one of the
favorable means to minimize the deleterious effects of the chemotherapy. Thus, this
study aimed to evaluate the preventive role of AgNPs synthesized by Ulva fasciata
(U. fasciata) against doxorubicin-induced hepatotoxicity and oxidative stress in
the liver of male Wistar rats. Materials and Methods: In the present study, the
green macroalga U. fasciata ethanolic extract was used as reducing agents to reduce
Ag ions to Ag-0. Doxorubicin-injected male Wistar rats were concomitantly treated
with U. fasciata ethanolic extract and AgNPs synthesized by U. fasciata extract
(AgNPs/U. fasciata) 3 times/week by oral gavage for 6 weeks. Results: The results
showed that male Wistar rats injected with doxorubicin showed a significant
increase in ALT, ALP and GGT activities and total bilirubin level as well as a
reduction in the serum albumin level. The concurrent treatments of doxorubicin-
injected rats with U. fasciata ethanolic extract and AgNPs/U. fasciata
significantly abrogate these alterations. The altered levels of tumor biomarkers
CA19.9 and AFP as well as pro-inflammatory cytokine, TNF-alpha, and anti-
inflammatory cytokine, IL-4, in doxorubicin-injected animals were significantly
ameliorated by concurrent treatment with U. fasciata and AgNPs/U. fasciata.
Moreover, the elevated mRNA expression of p53 significantly decreased by treatment.
In association, the doxorubicin-induced deleterious histological changes
represented by severe hydropic degenerative changes, steatosis, inflammatory cell
infiltration, Kupffer cell proliferation and apoptosis were remarkably improved by
concurrent treatment with U. fasciata extract and AgNPs/U. fasciata which was more
potent. Conclusion: Based on results of this study, it can be concluded that U.
fasciata extract and AgNPs/U. fasciata counteracts doxorubicin-induced toxicity by
suppression of inflammation, oxidative stress and apoptosis. AgNPs/U. fasciata was
the most potent in improving hepatocyte integrity and liver histological
architecture.
AN - rayyan-553780914
AU - Mahmoud, A. M.
AU - Ahmed, O. M.
AU - Mohamed, I. B.
AU - Soliman, H. A.
AU - Mohamed, B. M.
DO - 10.9734/JPRI/2021/v33i24A31429
IS - 24
KW - Rats
Rats, Wistar
Doxorubicin
PY - 2021
SN - 2456-9119
SP - 24-48
ST - The Preventive Effects and Mode of Actions of Ulva Fasciata Synthesized
Silver Nanoparticles in Doxorubicin-Induced Hepatotoxicity in Wistar Rats
TI - The Preventive Effects and Mode of Actions of Ulva Fasciata Synthesized
Silver Nanoparticles in Doxorubicin-Induced Hepatotoxicity in Wistar Rats
VL - 33
ID - 8927
ER -
TY - JOUR
AB - Progressive accumulation of misfolded amyloid proteins in intracellular and
extracellular spaces is one of the principal reasons for synaptic damage and
impairment of neuronal communication in several neurodegenerative diseases.
Effective treatments for these diseases are still lacking but remain the focus of
much active investigation. Despite testing several synthesized compounds, small
molecules, and drugs over the past few decades, very few of them can inhibit
aggregation of amyloid proteins and lessen their neurotoxic effects. Recently, the
natural polyphenol curcumin (Cur) has been shown to be a promising anti-amyloid,
anti-inflammatory and neuroprotective agent for several neurodegenerative diseases.
Because of its pleotropic actions on the central nervous system, including
preferential binding to amyloid proteins, Cur is being touted as a promising
treatment for age-related brain diseases. Here, we focus on molecular targeting of
Cur to reduce amyloid burden, rescue neuronal damage, and restore normal cognitive
and sensory motor functions in different animal models of neurodegenerative
diseases. We specifically highlight Cur as a potential treatment for Alzheimer’s,
Parkinson’s, Huntington’s, and prion diseases. In addition, we discuss the major
issues and limitations of using Cur for treating these diseases, along with ways of
circumventing those shortcomings. Finally, we provide specific recommendations for
optimal dosing with Cur for treating neurological diseases. © 2018 by the authors.
Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780915
AU - Maiti, P.
AU - Dunbar, G. L.
DO - 10.3390/ijms19061637
IS - 6
KW - Amyloidosis
Anti-amyloid
Curcumin
Molecular chaperones
Natural polyphenol
Neurodegenerative diseases
Neuroinflammation
Age Factors
Aging
Amyloid
Animals
Biological Products
Humans
Nanomedicine
Nanotechnology
Nerve Tissue
Neurodegenerative Diseases
Polyphenols
Signal Transduction
4,4' [(1 phenyl 1h pyrazole 3,5 diyl)diethene 2,1 diyl]bis(2 methoxyphenol)
adenine
alpha turmerone
amyloid protein
beta turmerone
calcium calmodulin dependent protein kinase
chitosan
copper
curcumin
curcuminoid
cyclodextrin
cytochrome
gold nanoparticle
guanine
hexahydrocurcumin
iron
monoamine oxidase inhibitor
octahydrocurcumin
phosphoprotein DARPP 32
piperine
polyphenol
polysaccharide
silver nanoparticle
tau interferon
tetrahydrocurcumin
turmerone
unclassified drug
unindexed drug
zinc
amyloid
biological product
age
allergy
apoptosis
biosynthesis
blood brain barrier
blood clotting disorder
brain hemorrhage
capillary endothelial cell
chest tightness
cholestasis
dermatitis
diarrhea
fluorescence
gastrointestinal symptom
gene expression
gene overexpression
lipid peroxidation assay
molecular biology
multiphoton microscopy
nausea
nervous system development
neuropathology
neurotoxicity
oligomerization
oxidative stress
pH
phagocytosis
protein aggregation
protein misfolding
rash
Review
signal transduction
synaptogenesis
temperature
aging
amyloidosis
animal
chemistry
dose response
drug effect
human
metabolism
nanomedicine
nanotechnology
nervous tissue
pathology
PY - 2018
ST - Use of curcumin, a natural polyphenol for targeting molecular pathways in
treating age-related neurodegenerative diseases
TI - Use of curcumin, a natural polyphenol for targeting molecular pathways in
treating age-related neurodegenerative diseases
85047960181&doi=10.3390%2fijms19061637&partnerID=40&md5=ce1805a6903f528e3f0c086d0f5
9e596
VL - 19
ID - 8928
ER -
TY - JOUR
AB - The phytocomponent conjugated silver nanoparticles (AgNPs) have been
extensively explored for various therapeutic applications such as antimicrobial,
antioxidant, anticancer, anti-inflammatory, antidiabetic and anticoagulant effects.
The bio-conjugation of Ag-based nanomaterial with plant extracts reduces their
toxicity to biological systems and enhances their therapeutic effectiveness. The
diversity of phytochemicals or capping agents provided by the plant extracts and
the small size and large surface area of AgNPs permits maximum adsorption of these
capping agents onto their surfaces that further promote the therapeutic performance
of phytoconjugated AgNPs in various biomedical applications. The mechanistic action
involved in antimicrobial and anticancer functions of AgNPs is mainly dependent on
the induction of reactive oxygen species (ROS) resulting in cellular apoptosis and
necrosis. This review summarizes the recent studies of various plant extract
assisted synthesis of AgNPs, potential biomedical applications with the possible
mechanism of action and major shortcomings affecting their therapeutic efficacy. ©
2021
AN - rayyan-553780916
AU - Majeed, M.
AU - Hakeem, K. R.
AU - Rehman, R. U.
DO - 10.1016/j.chemosphere.2021.132527
KW - Antimicrobial
Bio-conjugation
Efficacy
Nanomaterial
Phytochemicals
Therapeutic
Toxicity
Antioxidants
Metal Nanoparticles
Plant Extracts
Silver
Trachinotus falcatus
Cell death
Metal nanoparticles
Microorganisms
Plant extracts
plant extract
silver nanoparticle
antiinfective agent
antioxidant
metal nanoparticle
silver
Anti-inflammatories
Capping agent
Phytochemical
Phytocomponents
Synergistic effect
Therapeutic Application
anticoagulant
nanoparticle
anticoagulation
Article
drug efficacy
drug potentiation
human
nonhuman
synthesis
PY - 2022
ST - Synergistic effect of plant extract coupled silver nanoparticles in various
therapeutic applications- present insights and bottlenecks
T2 - Chemosphere
TI - Synergistic effect of plant extract coupled silver nanoparticles in various
therapeutic applications- present insights and bottlenecks
85116855608&doi=10.1016%2fj.chemosphere.2021.132527&partnerID=40&md5=e689c1d2182735
6cc941618e8ecbe90a
VL - 288
ID - 8929
ER -
TY - JOUR
AB - Noble metal nanoparticles, especially silver due to its antimicrobial
properties, are amongst the most widely used types of nanomaterials, and so the
possibility of an organism's exposure to them is relatively high. Excluding
injections, there are three natural routes they can accidentally enter the body the
skin, lungs and the alimentary tract. Research on rodents indicate that after
inhalation, injection or oral administration silver, gold and copper nanoparticles
can easily enter the systemic circulation and reach the internal organs.
Particularly vulnerable to the harmful effects of nanoparticles are organs with an
extended reticuloendothelial system, such as the spleen, where the accumulation of
nanoparticles occurs. It is well proved that metallic nanoparticles are easily
absorbed by macrophages located in lymphoid tissues but cannot be destroyed inside
the cells. They remain unchanged in phagosomes and chronically stimulate the cells
to pro-inflammatory cytokine production. They can also interact with other cell
types present in the local environment, e.g. lymphocytes, which can lead to an
inadequate immunological response of the organism. Many authors have described the
pro-inflammatory effect of noble metal nanoparticles, both local to the application
site and generalized. What is more, silver nanoparticles were able to disturb the
Th1/Th2 balance or even cause an allergic response of the organism. The beneficial
impact of silver nanoparticles on the immune response occurs only when they were
applied externally in the form of dressings or ointments in the treatment of
wounded or inflamed skin. In such cases nanosilver exhibited immunoregulatory
properties accelerating the healing. An explanation for this mode of action may be
the fact of relatively poor skin penetration by nanoparticles, limiting their
effect to the local tissues only.
AN - rayyan-553780917
AU - Malaczewska, J.
IS - 4
KW - Animals
Animal Shells
Immune System
PY - 2014
SN - 0025-8628
SP - 204-208
ST - Impact of noble metal nanoparticles on the immune system of animals
T2 - MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
TI - Impact of noble metal nanoparticles on the immune system of animals
VL - 70
Y2 - 4
ID - 8930
ER -
TY - JOUR
AB - An increasing number of applications of silver nanoparticles in industry,
medicine and everyday life means that the risk of exposure of the human organism to
their potential harmful influence is growing. This study has sought to assess the
effect of 28-day alimentary administration of different concentrations (0.25, 2.5
and 25 ppm) of a commercial silver nanocolloid on the proliferative activity and
synthesis of cytokines by mouse splenocytes. All of the analyzed doses of the
colloid had a significant, albeit different, effect on the activity of splenocytes.
At the lowest dose, a significant decrease in the proliferation of T cells and more
intensive synthesis of pro-inflammatory cytokines, both by non-stimulated and LPS-
stimulated cells, was observed. The intermediate dose, on the other hand,
stimulated proliferation of B cells while producing a pro-inflammatory effect
regarding the synthesis of cytokines. Finally, the highest dose decreased the
synthesis of cytoldnes by non-stimulated cells, but after LPS stimulation, through
the strong activation of the IL-10 synthesis, it raised the proliferation of B
cells and decreased the synthesis of pro-inflammatory cytokines. The results
suggest that silver nanoparticles administered orally have an easy access to the
peripheral organs of the immune system, such as the spleen, but the effect of long-
term exposure of this organ to the effect of silver nanocolloid depends on several
factors, including the dose of nanoparticles, and seems as difficult to predict.
AN - rayyan-553780918
AU - Malaczewska, J.
DO - 10.2478/pjvs-2014-0004
IS - 1
Mice
Cytokines
PY - 2014
SN - 1505-1773 2300-2557
SP - 27-35
ST - The splenocyte proliferative response and cytokine secretion in mice after
28-day oral administration of silver nanocolloid
T2 - POLISH JOURNAL OF VETERINARY SCIENCES
TI - The splenocyte proliferative response and cytokine secretion in mice after
28-day oral administration of silver nanocolloid
VL - 17
ID - 8931
ER -
TY - JOUR
AB - Nanostructured Ag@SiO2-Penicillin was synthesized from high-purity Ag0 NPs
with a mean particle size of about 10 nm produced by electromagnetic levitation gas
condensation (ELGC) method. The silver and penicillin contents of the synthesized
nano-antibiotic were about 34 wt% and 2.5 wt% respectively, as determined by ICP-
OES and TGA analyses. The antibacterial properties and synergistic effects of
nanostructured Ag@SiO2 and Ag@SiO2–Penicillin on killing the Methicillin-
susceptible S. aureus (MSSA) and Methicillin-resistant S. aureus (MRSA) bacteria
were also examined. The nanoparticles were characterized by X-ray diffraction (XRD)
and transmission electron microscopy (TEM). Ag@SiO2-Penicillin NPs showed an
outstanding antibacterial activity compared to Penicillin and Ag@SiO2 NPs. The
Fractional inhibitory concentration (FIC) indexes were 0.54 and 0.52 against MSSA
and MRSA bacteria respectively, illustrating the synergistic effects of Ag@SiO2-
Penicillin NPs. In addition, Ag@SiO2-Penicillin NPs showed promising dose-dependent
cytotoxicity effects indicating the protective effects emanating from anti-
inflammatory properties of penicillin. © 2019 Elsevier B.V.
AN - rayyan-553780920
AU - Malekzadeh, M.
AU - Yeung, K. L.
AU - Halali, M.
AU - Chang, Q.
DO - 10.1016/j.msec.2019.04.083
KW - Penicillin
Silica
Synergistic effect
Cell Death
Cell Survival
Electromagnetic Phenomena
Humans
Nanostructures
Nanotechnology
Particle Size
Penicillins
Silicon Dioxide
Silver
Static Electricity
Thermogravimetry
X-Ray Diffraction
Bacteria
Drug products
Electromagnetic propulsion
Levitation melting
Nanoparticles
Particle size
nanomaterial
silicon dioxide
silver
Anti-inflammatories
Cytotoxicity effects
Electromagnetic levitation
Inhibitory concentration
cell death
cell survival
chemistry
drug effect
electromagnetism
human
nanotechnology
particle size
procedures
static electricity
synthesis
thermogravimetry
ultrastructure
X ray diffraction
Silver compounds
Penicillin V
Penicillin G
PY - 2019
SP - 616-622
ST - Synthesis of nanostructured Ag@SiO2-Penicillin from high purity Ag NPs
prepared by electromagnetic levitation melting process
TI - Synthesis of nanostructured Ag@SiO2-Penicillin from high purity Ag NPs
prepared by electromagnetic levitation melting process
85065037195&doi=10.1016%2fj.msec.2019.04.083&partnerID=40&md5=21d553929bd524d06b048
667786aac97
VL - 102
ID - 8933
ER -
TY - JOUR
AB - Aim of the study The present study was aimed to evaluate the anti-arthritic
effects of silver nanoparticles synthesised using Piper nigrum extract and to
further establish its mechanism of action in a rat model of adjuvant induced
arthritis (AA). Materials and methods Adjuvant arthritis was induced by injecting
complete Freund's adjuvant (0.1 mL) into the left hind paw of 36 albino Wistar rats
(n = 6). Silver nanoparticles stabilised with Piper nigrum extract (25 and
50 mg/kg). Commercial silver nanoparticles (50 mg/kg) and methotrexate (0.1 mg/kg)
were administered by intraperitoneal route from day 11 to day 22 on alternate days.
Results It was found that treatment with silver nanoparticles stabilised with Piper
nigrum (S-AgNPs) significantly reduced the paw edema and alleviated the
histopathological changes of cell infiltration, synovial hyperplasia, bone and
cartilage destruction. Furthermore, the phytostabilised silver nanoparticles (S-
AgNPs) inhibited the protein expression of NF-kβ p65 and TNF-α as evidenced by
immunohistochemistry analysis. Conclusion Our current findings suggest that silver
nanoparticles stabilised with Piper nigrum extract (S-AgNPs) have potent anti-
arthritic activity which is mediated by inhibition of TNF-α and suppression of pro-
inflammatory cytokines that are secreted in response to activated transcription
factors of NF-kβ. © 2016 Elsevier B.V.
AN - rayyan-553780921
AU - Mani, A.
AU - Vasanthi, C.
AU - Gopal, V.
AU - Chellathai, D.
DO - 10.1016/j.intimp.2016.10.013
KW - Adjuvant arthritis
NF-kβ
Piper nigrum
TNF-α
Animals
Drug Stability
Edema
Foot Joints
Freund's Adjuvant
Fruit
Lymph Nodes
Male
Metal Nanoparticles
Phytotherapy
Piper
Plant Extracts
Rats, Wistar
Silver
Transcription Factor RelA
black pepper extract
creatinine
Freund adjuvant
methotrexate
silver nanoparticle
transcription factor RelA
urea
metal nanoparticle
plant extract
silver
adjuvant arthritis
alkaline phosphatase blood level
animal cell
animal experiment
animal model
animal tissue
antiarthritic activity
Article
bone erosion
cartilage degeneration
cell hyperplasia
cell infiltration
controlled study
drug mechanism
drug synthesis
hind paw
histopathology
hyperplasia
liver toxicity
male
nonhuman
particle size
paw edema
priority journal
protein expression
rat
zeta potential
animal
chemistry
drug effects
drug stability
edema
foot joint
fruit
lymph node
metabolism
pathology
phytotherapy
Piper (plant)
Wistar rat
Rats
PY - 2016
SP - 17-23
ST - Role of phyto-stabilised silver nanoparticles in suppressing adjuvant induced
arthritis in rats
TI - Role of phyto-stabilised silver nanoparticles in suppressing adjuvant induced
arthritis in rats
84992364820&doi=10.1016%2fj.intimp.2016.10.013&partnerID=40&md5=a227ae49074dd35326f
0df080efe6399
VL - 41
ID - 8934
ER -
TY - JOUR
AB - Biologically inspired synthesis of nanoparticles was found to be more
attractive in metal nanoparticle synthesis. The present study reported an in-situ
biogenic synthesis of silver nanoparticles (AgNPs) using Solanum trilobatum aqueous
leaf extract. On this basis, the aqueous leaf extract of S. trilobatum acted as a
reducing agent and stabilizing agent to synthesize highly stable AgNPs at ambient
temperature. Eventually, the synthesized and stabilized AgNPs surface plasmon
resonance was near 430 nm through a UV-visible (UV-vis) spectrophotometer. Here,
the stability of the silver colloids monitored through zeta potential and mean
particle size was evaluated through diffraction light scattering (DLF). Further,
the average particle size was found to be 27.6 nm and spherical, confirmed with
transmission electron microscopy (TEM). Also, colloidal AgNPs and aqueous extract
are found to be rich sources of antioxidants and exhibit higher free radical
scavenging ability. Thus, efficient inhibition with COX1 and COX2 enzymes and the
protective effect with human red blood cell (HRBC) membrane stability showed
significant results. These features are promising, suggesting the possibility of
the AgNPs to be useful to disease-modifying for treating inflammatory disorders and
associated complications. (C) 2021 The Author(s). Published by Elsevier B.V. on
behalf of King Saud University.
AN - rayyan-553780923
AU - Manimegalai, S.
AU - Rajeswari, V. D.
AU - Parameswari, R.
AU - Nicoletti, M.
AU - Alarifi, S.
AU - Govindarajan, M.
DO - 10.1016/j.sjbs.2021.11.048
IS - 4
PY - 2022
SN - 1319-562X 2213-7106
SP - 2131-2137
ST - Green synthesis, characterization and biological activity of Solanum
trilobatum-mediated silver nanoparticles
TI - Green synthesis, characterization and biological activity of Solanum
trilobatum-mediated silver nanoparticles
VL - 29
Y2 - 4
ID - 8936
ER -
TY - JOUR
AB - The primary objective of the current investigation was the biosynthesis of
Phy-AgNPs by the endophytic fungus Phyllosticta owaniana (extracted from Abrus
precatorius) and the evaluation of the secondary metabolites from the ethyl acetate
extract of P. owaniana cultivated by submerged fermentation. Utilizing
bioanalytical strategies, Phy-AgNPs were characterized. The UV–visible
spectrophotometer analysis revealed an absorption spectrum with a peak at 420 nm,
thus validating the Phy-AgNPs synthesis. The FTIR analysis revealed peaks
correlating to various potential functional groups, suggesting that Phy-AgNPs have
been reduced and capped. SEM-EDAX and HR-TEM analyses demonstrated the spherical
shape of Phy-AgNPs, and the 3 keV EDAX analysis confirmed the existence of silver
atoms. XRD analyses showed the Phy-AgNPs crystalline structure. The size and the
stability of synthesized Phy-AgNPs (65.81 nm) were measured by DLS and Zeta
potential studies. While the ethyl acetate extract was analyzed with GC–MS and FTIR
for secondary metabolites. The synthesized Phy-AgNPs showed effective antibacterial
activity against Pseudomonas aeruginosa (15.1 ± 0.17 mm, 10 mg/mL), while the
antifungal activity of Phy-AgNPs inhibited the growth of Candida albicans extremely
efficiently (12.16 ± 0.28 mm, 10 mg/mL). Phy-AgNPs were evaluated for a variety of
biomedical properties in which they showed significant activity. In a cell
viability assay using the MTT assay, Phy-AgNPs exhibited a cytotoxic impact of up
to 30.67% and 34.53% when 200 µg/mL were detected. In both in vitro and in vivo
anti-inflammatory examinations, nanoparticles (NPs) exhibited a significant anti-
inflammatory effect. These findings support the pharmaceutical and biomedical
properties of the synthesized Phy-AgNPs. © 2023, The Author(s), under exclusive
licence to Springer-Verlag GmbH Germany, part of Springer Nature.
AN - rayyan-553780924
AU - Manjunatha, D.
AU - Megha, G. T.
AU - Nagaraju, S.
AU - Akarsh, S.
AU - Nandish, G.
AU - Sowmya, H. V.
AU - Thippeswamy, B.
DO - 10.1007/s00203-023-03549-1
IS - 5
KW - Antimicrobial properties
Endophytic fungus
GC–MS
Phy-AgNPs
Phyllosticta owaniana
Fungi
Metal Nanoparticles
Plant Extracts
Silver
amoxicillin
ampicillin
ascorbic acid
cefpodoxime
chloramphenicol
ciprofloxacin
cisplatin
diclofenac
fluconazole
functional group
fungal extract
gentamicin
imipenem
silver nanoparticle
silver nitrate
streptomycin
antiinfective agent
metal nanoparticle
plant extract
silver
Abrus precatorius
absorption spectroscopy
animal experiment
animal model
animal tissue
antifungal activity
Article
Aspergillus brasiliensis
Aspergillus flavus
bacterial growth
bacterium culture
biosynthesis
Candida albicans
carrageenan-induced paw edema
cell viability
controlled study
crystal structure
cytotoxicity
drug synthesis
endophytic fungus
Escherichia coli
evaluation study
female
hemolysis
high resolution transmission electron microscopy
histopathology
human
human cell
in vitro study
in vivo study
membrane stabilization
molecular fingerprinting
mouse
MTT assay
muscle hypotonia
nonhuman
particle size
protein denaturation
secondary metabolism
submerged fermentation
X ray diffraction
zeta potential
zone of inhibition
chemistry
fungus
PY - 2023
ST - Eco-friendly synthesized silver nanoparticles from endophytic fungus
Phyllosticta owaniana: KUMBMDBT-32 and evaluation of biomedical properties
T2 - Archives of Microbiology
TI - Eco-friendly synthesized silver nanoparticles from endophytic fungus
Phyllosticta owaniana: KUMBMDBT-32 and evaluation of biomedical properties
85159542280&doi=10.1007%2fs00203-023-03549-
1&partnerID=40&md5=f2833c74308d018dca22aa48e31a4307
VL - 205
ID - 8937
ER -
TY - JOUR
AB - Nanoparticles (NP) are highly applicable in a variety of technological and
biomedical fields because of their unique physicochemical properties. The increased
development and utilization of NP has amplified human exposure and raised concerns
regarding their potential to generate toxicity. The biological impacts of NP
exposures have been shown to be dependent on aerodynamic size, chemical
composition, and the route of exposure (oral, dermal, intravenous, and inhalation),
while recent research has demonstrated the cardiovascular (CV) system as an
important site of toxicity. Proposed mechanisms responsible for these effects
include inflammation, oxidative stress, autonomic dysregulation, and direct
interactions of NP with CV cells. Specifically, NP have been shown to impact
vascular endothelial cell (EC) integrity, which may disrupt the dynamic endothelial
regulation of vascular tone, possibly altering systemic vascular resistance and
impairing the appropriate distribution of blood flow throughout the circulation.
Cardiac consequences of NP-induced toxicity include disruption of heart rate and
electrical activity via catecholamine release, increased susceptibility to
ischemia/reperfusion injury, and modified baroreceptor control of cardiac function.
These and other CV outcomes likely contribute to adverse health effects promoting
myocardial infarction, hypertension, cardiac arrhythmias, and thrombosis. This
review will assess the current knowledge regarding the principle sites of CV
toxicity following NP exposure. Furthermore, we will propose mechanisms
contributing to altered CV function and hypothesize possible outcomes resulting in
decrements in human health. © 2012 Wiley Periodicals, Inc.
AN - rayyan-553780925
AU - Mann, E. E.
AU - Thompson, L. C.
AU - Wingard, C. J.
DO - 10.1002/wnan.1194
IS - 6
KW - Animals
Cardiovascular Diseases
Cardiovascular Physiological Phenomena
Cardiovascular System
Humans
Inhalation Exposure
Nanoparticles
Blood pressure
Diseases
Endothelial cells
advanced glycation end product receptor
catecholamine
cerium oxide
G protein coupled receptor
interleukin 1beta
interleukin 6
iron oxide
messenger RNA
nanoparticle
nickel complex
nitric oxide
prostaglandin E2
prostaglandin synthase
silicon dioxide
silver nanoparticle
thromboxane A2
titanium dioxide
zinc oxide
Adverse health effects
Aerodynamic sizes
Biological impacts
Biomedical fields
Blood flow
Cardiac arrhythmia
Cardiac functions
Cardio-pulmonary function
Chemical compositions
Direct interactions
Electrical activities
Heart rates
Human exposures
Human health
Ischemia/reperfusion
Myocardial Infarction
Vascular endothelial cells
Vascular tones
article
autonomic dysfunction
blood flow
blood vessel tone
cardiopulmonary function
cardiotoxicity
catecholamine release
cytotoxicity
electric activity
exposure
health hazard
heart arrhythmia
heart muscle ischemia
heart rate
human
hypertension
inflammation
lung toxicity
nonhuman
outcome assessment
oxidative stress
particle size
pressoreceptor
priority journal
reperfusion injury
thrombosis
vascular endothelium
vascular resistance
vascular smooth muscle
Toxicity
PY - 2012
SP - 691-702
ST - Changes in cardiopulmonary function induced by nanoparticles
T2 - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
TI - Changes in cardiopulmonary function induced by nanoparticles
84867698123&doi=10.1002%2fwnan.1194&partnerID=40&md5=5dc35bb5ba9ee2fe791d9c164fb535
c3
VL - 4
ID - 8938
ER -
TY - JOUR
AB - Nanoparticles (NPs) are tiny materials used in a wide range of industrial and
medical applications. Titanium dioxide (TiO2) is a type of nanoparticle that is
widely used in paints, pigments, and cosmetics; however, little is known about the
impact of TiO2 on human health and the environment. Therefore, considerable
research has focused on characterizing the potential toxicity of nanoparticles such
as TiO2 and on understanding the mechanism of TiO2 NP-induced nanotoxicity through
the evaluation of biomarkers. Uncoated TiO2 NPs tend to aggregate in aqueous media,
and these aggregates decrease cell viability and induce expression of stress-
related genes, such as those encoding interleukin-6 (IL-6) and heat shock protein
70B' (HSP70B'), indicating that TiO2 NPs induce inflammatory and heat shock
responses. In order to reduce their toxicity, we conjugated TiO2 NPs with
polyethylene glycol (PEG) to eliminate aggregation. Our findings indicate that
modifying TiO2 NPs with PEG reduces their cytotoxicity and reduces the induction of
stress-related genes. Our results also suggest that TiO2 NP-induced effects on
cytotoxicity and gene expression vary depending upon the cell type and surface
modification.
AN - rayyan-553780926
AU - Mano, S. S.
AU - Kanehira, K.
AU - Sonezaki, S.
AU - Taniguchi, A.
DO - 10.3390/ijms13033703
IS - 3
KW - Humanities
Humanism
Humans
Glycols
Gene Expression
Cell Line
PY - 2012
SN - 1422-0067
SP - 3703-3717
ST - Effect of Polyethylene Glycol Modification of TiO2 Nanoparticles on
Cytotoxicity and Gene Expressions in Human Cell Lines
TI - Effect of Polyethylene Glycol Modification of TiO2 Nanoparticles on
Cytotoxicity and Gene Expressions in Human Cell Lines
VL - 13
Y2 - 3
ID - 8939
ER -
TY - JOUR
AB - Nano metal oxides have been proposed as alternatives to silver (Ag)
nanoparticles (NPs) for antibacterial coatings. Here, cotton and polyester-cotton
fabrics were sonochemically coated with zinc oxide (ZnO) and copper oxide (CuO)
NPs. By varying the reaction solvent (water or ethanol), NPs with different sizes
and shapes were synthesized. The cytotoxic and pro-inflammatory effects of studied
NPs were investigated in vitro in human alveolar epithelial A549 and macrophage-
like THP1 cells. To understand the potential respiratory impact of the NPs, the
coated textiles were subjected to the abrasion tests, and the released airborne
particles were measured. A very small amount of the studied metal oxides NPs was
released from abrasion of the textiles coated by the ethanol-based sonochemical
process. The release from the water-based coating was comparably higher. Lung and
immune cells viability decreased after 24 h of exposure only at the highest studied
NPs concentration (100 mu g/mL). Different from the ZnO NPs, both formulations of
CuO NPs induced IL-8 release in the lung epithelial cells already at subtoxic
concentrations (1-10 mu g/mL) but not in immune cells. All of the studied NPs did
not induce IL-6 release by the lung and immune cells. Calculations revealed that
the exposures of the NPs to human lung due to the abrasion of the textiles were
lower or comparable to the minimum doses in the cell viability tests (0.1 mu g/mL),
at which acute cytotoxicity was not observed. The results alleviate the concerns
regarding the potential risk of these metal oxide NPs in their applications for the
textile coating and provide insight for the safe-by-design approach.
AN - rayyan-553780929
AU - Mantecca, P.
AU - Kasemets, K.
AU - Deokar, A.
AU - Perelshtein, L.
AU - Gedanken, A.
AU - Bahk, Y. K.
AU - Kianfar, B.
AU - Wang, J.
DO - 10.1021/acs.est.7b02390
IS - 16
KW - Nanoparticles
PY - 2017
SN - 0013-936X 1520-5851
SP - 9305-9317
ST - Airborne Nanoparticle Release and Toxicological Risk from Metal-Oxide-Coated
Textiles: Toward a Multiscale Safe-by-Design Approach
T2 - ENVIRONMENTAL SCIENCE & TECHNOLOGY
TI - Airborne Nanoparticle Release and Toxicological Risk from Metal-Oxide-Coated
Textiles: Toward a Multiscale Safe-by-Design Approach
VL - 51
Y2 - 8 y3 - 15
ID - 8941
ER -
TY - JOUR
AB - Background:This investigation aimed to evaluate the occurrence of some
apoptotic features induced by Leptospira interrogans serovar Icterohaemorrhagiae
infection in young BALB/c mice during 2, 4, 7, 10, 14 and 21 days post-infection
(dpi).Methods:The animals were euthanized and lung, liver and kidneys were
harvested to histopathology analysis and immunohistochemistry to caspase-3 antigen
detection was performed.Results:Chromatin condensation in kidney and liver tissues,
but not in lung tissue, was observed. Caspase-3 reactive cells, mainly
characterized as renal epithelial cells, were detected in the days 14 and 21 at
high levels when compared to days 2,4 and 7 (p = 0.025; p <0.05). Lung sections
revealed caspase-3 labeled alveolar cells in 10 and 14 days post-infection was
higher than observed at 7 days (p = 0.0497; p < 0.05). Liver sections demonstrated
reactive cells at a highest level at 14 and 21 days post-infection when comparison
to 2,4, 7 and 10 days (p = 0.0069; p<0.05).Conclusions:Our results suggest that
infection of L interrogans induce in kidney, liver and lung an activation of
apoptosis mediated by caspase-3 dependent pathway in later phases of infectious
process.(AU)
AN - rayyan-553780930
AU - Marinho, Márcia
AU - Táparo, Cilene Vidovix
AU - Oliveira Júnior, Itamar S.
AU - Perri, Silvia Helena Venturoli
AU - Cardoso, Tereza Cristina
DO - 10.1186/s40409-015-0022-y
KW - Caspases
Leptospirosis
Mice
Programmed cell death
Apoptosis
LA - en
PY - 2015
SN - 1678-9199
SP - 22-22
ST - Tissue apoptosis in mice infected with Leptospira interrogans serovar
Icterohaemorrhagiae
T2 - J. venom. anim. toxins incl. trop. dis
TI - Tissue apoptosis in mice infected with Leptospira interrogans serovar
Icterohaemorrhagiae
UR - https://www.scielo.br/j/jvatitd/a/MHJhLZNZdn7Mb3p7rnCzGRb/?format=pdf〈=en
VL - 21
ID - 8942
ER -
TY - JOUR
AB - Recent studies demonstrated that inhibitors of pro-inflammatory molecular
cascades triggered by rabies infection in the central nervous system (CNS) can
enhance survival in mouse model and that certain antiviral compounds interfere with
rabies virus replication in vitro. In this study different combinations of
therapeutics were tested to evaluate their effect on survival in rabies-infected
mice, as well as on viral load in the CNS. C57Bl/6 mice were infected with Silver-
haired bat rabies virus (SHBRV)-18 at virus dose approaching LD50 and LD100. In one
experimental group daily treatments were initiated 4 h before-, in other groups 48
or 96 h after challenge. In the first experiment therapeutic combination contained
inhibitors of tumour necrosis factor-α (infliximab), caspase-1 (Ac-YVAD-cmk), and a
multikinase inhibitor (sorafenib). In the treated groups there was a notable but
not significant increase of survival compared to the virus infected, non-treated
mice. The addition of human rabies immunoglobulins (HRIG) to the combination in the
second experiment almost completely prevented mortality in the pre-exposure
treatment group along with a significant reduction of viral titres in the CNS.
Post-exposure treatments also greatly improved survival rates. As part of the
combination with immunomodulatory compounds, HRIG had a higher impact on survival
than alone. In the third experiment the combination was further supplemented with
type-I interferons, ribavirin and favipiravir (T-705). As a blood-brain barrier
opener, mannitol was also administered. This treatment was unable to prevent lethal
consequences of SHBRV-18 infection; furthermore, it caused toxicity in treated
mice, presumably due to interaction among the components. In all experiments, viral
loads in the CNS were similar in mice that succumbed to rabies regardless of
treatment. According to the findings, inhibitors of detrimental host response to
rabies combined with antibodies can be considered among the possible therapeutic
and post-exposure options in human rabies cases. © 2018 Elsevier Ltd
AN - rayyan-553780931
AU - Marosi, A.
AU - Dufkova, L.
AU - Forró, B.
AU - Felde, O.
AU - Erdélyi, K.
AU - Širmarová, J.
AU - Palus, M.
AU - Hönig, V.
AU - Salát, J.
AU - Tikos, R.
AU - Gyuranecz, M.
AU - Růžek, D.
AU - Martina, B.
AU - Koraka, P.
AU - Osterhaus, A. D. M. E.
AU - Bakonyi, T.
DO - 10.1016/j.vaccine.2018.05.066
IS - 33
KW - Combination
HRIG
Infliximab
Rabies virus
Sorafenib
Survival
Animals
Antibodies, Viral
Antiviral Agents
Body Weight
Female
Immunoglobulins
Kaplan-Meier Estimate
Mice
Mice, Inbred C57BL
Rabies
Real-Time Polymerase Chain Reaction
Virus Replication
acetyltyrosylvalylalanylaspartic acid chloromethylketone
alpha interferon
antivirus agent
beta interferon
favipiravir
infliximab
interferon
rabies immunoglobulin
ribavirin
sorafenib
unclassified drug
immunoglobulin
virus antibody
animal experiment
animal model
animal tissue
Article
blood brain barrier
C57BL 6 mouse
combination drug therapy
controlled study
drug antagonism
female
immune response
immunomodulation
LD100
LD50
monotherapy
mortality
mouse
nonhuman
priority journal
rabies
survival rate
virus load
animal
body weight
C57BL mouse
disease model
drug effect
immunology
Kaplan Meier method
pathogenicity
virus replication
Humanities
Humanism
Humans
Immunoglobulin D
Immunoglobulin M
Immunoglobulin A
Immunoglobulin G
Immunoglobulin E
PY - 2019
SP - 4724-4735
ST - Combination therapy of rabies-infected mice with inhibitors of pro-
inflammatory host response, antiviral compounds and human rabies immunoglobulin
T2 - Vaccine
TI - Combination therapy of rabies-infected mice with inhibitors of pro-
inflammatory host response, antiviral compounds and human rabies immunoglobulin
85047466499&doi=10.1016%2fj.vaccine.2018.05.066&partnerID=40&md5=c9094d4a0a7042bb3f
0bd48529cc96a0
VL - 37
ID - 8943
ER -
TY - JOUR
AB - PURPOSE: To evaluate intestinal inflammatory and apoptotic processes after
intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic
saline. METHODS: It was allocated into four groups (n=6), 24 male Wistar rats (200
to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80
min: IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV);
PTX group (Pentoxifylline, 30mg/kg-IV); HS+PTX group (Hypertonic Saline and
Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion,
ileal fragments were removed and stained on hematoxylin-eosin and histochemical
studies for COX-2, Bcl-2 and cleaved caspase-3. RESULTS: The values of sO2 were
higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of
reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40
minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098).
Morphologic tissue injuries showed higher grades on IR group versus other groups:
HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed
lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower
expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR).
CONCLUSION: The combined use of pentoxifylline and hypertonic saline offers best
results on inflammatory and apoptotic inhibitory aspects after intestinal
ischemia/reperfusion. .
AN - rayyan-553780932
AU - Marques, Geraldo Magela Nogueira
AU - Rasslan, Roberto
AU - Belon, Alessandro Rodrigo
AU - Carvalho, Juliana Gonçalves
AU - Felice Neto, Raphael
AU - Rasslan, Samir
AU - Utiyama, Edivaldo Massazo
AU - Montero, Edna Frasson de Souza
DO - 10.1590/S0102-86502014001800007
IS - 11
KW - Apoptosis
Inflammation
Ischemia
Pentoxifylline
Rats
Reperfusion
Saline Solution, Hypertonic
LA - en
PY - 2014
SN - 0102-8650
SP - 735-741
ST - Pentoxifylline associated to hypertonic saline solution attenuates
inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats
T2 - Acta cir. bras
TI - Pentoxifylline associated to hypertonic saline solution attenuates
inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats
86502014001100735
VL - 29
Y2 - 11 y3 - 1
ID - 8944
ER -
TY - JOUR
AB - Background Indian-origin rhesus (InR) are preferred for research, but strict
export restrictions continue to limit their use. Chinese-origin rhesus (ChR),
although easier to procure, are genetically distinct from InR and differ in their
immune response to infectious agents, such as the Simian Immunodeficiency Virus.
The most advanced malaria vaccine, RTS,S (GlaxoSmithKline), is based on the
circumsporozoite protein (CSP) of Plasmodium falciparum. The efficacy of RTS,S
vaccine in the field remains low and short-lived; efforts are underway to improve
CSP-based vaccines. Rhesus models can accelerate preclinical down-selection of the
next generation of malaria vaccines. This study was used to determine if the safety
and immunogenicity outcomes following vaccination with a CSP vaccine would differ
in the InR and ChR models, given the genetic differences between the two sub-
populations of rhesus. Methods The FMP013 vaccine, was composed of nearly full-
length soluble P. falciparum CSP produced in Escherichia coli and was adjuvanted
with the Army liposomal formulation (ALFQ). Three doses of the vaccine were
administered in InR and ChR (n = 6) at 1-month intervals and the antibody and T
cell responses were assessed. Results Local and systemic toxicity profile of FMP013
vaccine in InR and ChR were similar and they revealed that the FMP013 vaccine was
safe and caused only mild and transient inflammatory adverse reactions. Following
the first 2 vaccines, there was a slower acquisition of antibodies to the CSP
repeat region in ChR. However after the 3rd vaccination the titers in the two
models were comparable. The ChR group repeat-specific antibodies had higher avidity
and ChR group showed higher inhibition of liver stage development activity compared
to InR. There was no difference in T-cell responses to the FMP013 vaccine between
the two models. Conclusions A difference in the quality of serological responses
was detected between the two sub-populations of rhesus. However, both models
confirmed that FMP013/ALFQ vaccine was safe, highly immunogenic, elicited
functional antibodies and T-cell responses. Overall, the data suggests that rhesus
of Indian and Chinese origins can be interchangeably used to compare the safety and
immunogenicity of next-generation of malaria vaccines and adjuvants.
AN - rayyan-553780933
AU - Martin, M. L.
AU - Bitzer, A. A.
AU - Schrader, A.
AU - Bergmann-Leitner, E. S.
AU - Soto, K.
AU - Zou, X. Y.
AU - Beck, Z.
AU - Matyas, G. R.
AU - Dutta, S.
DO - 10.1186/s12936-019-3014-5
IS - 1
KW - Vaccination
Macaca mulatta
Malaria
Vaccines
PY - 2019
SN - 1475-2875
ST - Comparison of immunogenicity and safety outcomes of a malaria vaccine
FMP013/ALFQ in rhesus macaques (Macaca mulatta) of Indian and Chinese origin
T2 - MALARIA JOURNAL
TI - Comparison of immunogenicity and safety outcomes of a malaria vaccine
FMP013/ALFQ in rhesus macaques (Macaca mulatta) of Indian and Chinese origin
VL - 18
Y2 - 11 y3 - 27
ID - 8945
ER -
TY - JOUR
AB - AIM: Infections associated with medical devices are an important cause of
morbidity and mortality. Microorganisms are responsible for catheter infections
that may then result in the local or systemic dissemination of the microorganism
into the bloodstream. The aim of this study was to evaluate the antimicrobial
activity of silver nanoparticles (AgNPs) embedded in polyurethane plastics,
commonly used for catheter fabrication. MATERIALS & METHODS: AgNPs in the range of
25-30 nm were synthesized and embedded in polyurethane plastics at different
concentrations. The antimicrobial activities of these plastics were tested against
the three pathogenic microorganisms, Escherichia coli, Staphylococcus epidermidis
and Candida albicans, frequently associated with catheter infections. The
cytotoxicity of the plastics was evaluated on human-derived macrophages using
propidium iodide and the secretion of the pro- and anti-inflammatory cytokines IL-
6, IL-10 and TNF-a was measured using ELISA. RESULTS: A significant reduction of 6-
to 7-log in the number of bacteria was measured, while a reduction of 90% was
measured in the case of C. albicans. Neither cytotoxic effect on macrophages nor
immunological response was observed. CONCLUSION: Plastics embedded with AgNPs have
great potential to limit microbial colonization of implanted medical devices.
AN - rayyan-553781836
AU - Martínez-Gutiérrez, F.
AU - Guajardo-Pacheco, J. M.
AU - Noriega-Trevino, M. E.
AU - Thi, E. P.
AU - Reiner, N.
AU - Orrantia, E.
AU - Av-Gay, Y.
AU - Ruiz, F.
AU - Bach, H.
DO - 10.2217/fmb.13.5
IS - 3
J2 - Future Microbiol
KW - Anti-Infective Agents/*pharmacology
Apoptosis
Candida albicans/drug effects
Cell Line
Enzyme-Linked Immunosorbent Assay
Humans
Macrophages/drug effects
Nanoparticles/*toxicity
Polyurethanes/*pharmacology/*toxicity
Silver/*pharmacology/*toxicity
Staphylococcus epidermidis/drug effects
Tumor Necrosis Factor-alpha/metabolism
LA - eng
N1 - Department of Medicine, Division of Infectious Diseases, University of
British Columbia, Vancouver, BC, Canada.
PY - 2013
SP - 403-11
ST - Antimicrobial activity, cytotoxicity and inflammatory response of novel
plastics embedded with silver nanoparticles
T2 - Future microbiology
TI - Antimicrobial activity, cytotoxicity and inflammatory response of novel
plastics embedded with silver nanoparticles
VL - 8
Y2 - 3
ID - 9763
ER -
TY - JOUR
AB - The incorporation of nanoparticles (NPs) in industrial and biomedical
applications has increased significantly in recent years, yet their hazardous and
toxic effects have not been studied extensively. Here, we studied the effects of 24
nm silver NPs (AgNPs) on a panel of bacteria isolated from medical devices used in
a hospital intensive care unit. The cytotoxic effects were evaluated in macrophages
and the expression of the inflammatory cytokines IL-6, IL-10 and TNF-α were
quantified. The effects of NPs on coagulation were tested in vitro in plasma-based
assays. We demonstrated that 24 nm AgNPs were effective in suppressing the growth
of clinically relevant bacteria with moderate to high levels of antibiotic
resistance. The NPs had a moderate inhibitory effect when coagulation was initiated
through the intrinsic pathway. However, these NPs are cytotoxic to macrophages and
are able to elicit an inflammatory response. Thus, beneficial and potential harmful
effects of 24 nm AgNPs on biomedical devices must be weighed in further studies in
vivo. From the Clinical Editor: The authors of this study demonstrate that gallic
acid reduced 24 nm Ag NPs are effective in suppressing growth of clinically
relevant antibiotic resistant bacteria. However, these NPs also exhibit cytotoxic
properties to macrophages and may trigger an inflammatory response. Thus, the
balance of beneficial and potential harmful effects must be weighed carefully in
further studies.
AN - rayyan-553781809
AU - Martínez-Gutierrez, F.
AU - Thi, E. P.
AU - Silverman, J. M.
AU - de Oliveira, C. C.
AU - Svensson, S. L.
AU - Vanden Hoek, A.
AU - Sánchez, E. M.
AU - Reiner, N. E.
AU - Gaynor, E. C.
AU - Pryzdial, E. L.
AU - Conway, E. M.
AU - Orrantia, E.
AU - Ruiz, F.
AU - Av-Gay, Y.
AU - Bach, H.
DO - 10.1016/j.nano.2011.06.014
IS - 3
J2 - Nanomedicine
KW - Anti-Bacterial Agents/*pharmacology
Bacteria/drug effects
Blood Coagulation/*drug effects
Cell Death/drug effects
Cell Line
Humans
Inflammation/*pathology
Light
Macrophages/cytology/drug effects
Metal Nanoparticles/*toxicity/ultrastructure
Particle Size
Scattering, Radiation
Silver/*pharmacology/*toxicity
LA - eng
N1 - Department of Medicine, Division of Infectious Diseases, University of
British Columbia, Vancouver, BC, Canada; Facultad de Ciencias Químicas, UASLP,
Álvaro Obregón 64, San Luis Potosí, México; Centro de Investigaciones de Materiales
Avanzados, Chihuahua, México.
PY - 2012
SP - 328-36
ST - Antibacterial activity, inflammatory response, coagulation and cytotoxicity
effects of silver nanoparticles
T2 - Nanomedicine : nanotechnology, biology, and medicine
TI - Antibacterial activity, inflammatory response, coagulation and cytotoxicity
effects of silver nanoparticles
VL - 8
Y2 - 4
ID - 9739
ER -
TY - JOUR
AB - Biomaterials are often used in orthopedic surgery like cavity fillings.
However, related complications often require long-term systemic antibiotics, device
removal, and extended rehabilitation. Hydroxyapatite/silver (HA/Ag) composites have
been proposed as implantation biomaterials owing to the osteogenic properties of
hydroxyapatite and to the antimicrobial efficiency of silver. Nevertheless, higher
silver concentrations induce cytotoxic effects. The aim of this study was to
synthesize and characterize HA/Ag nanocomposites that will allow us to use lower
concentrations of silver nanoparticles with better antimicrobial efficiency and
anti-inflammatory properties. The characterization of HA/Ag was performed by
scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction,
Fourier-transform infrared spectra, X-ray photoelectron spectroscopy, and laser
diffraction. Bioactivity was evaluated under a simulated body fluid. The viability
of osteoblast like-cells (MG-63) was determined by MTT (3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyl-2H-tetrazolium bromide) and the antimicrobial activity was
evaluated by the standard McFarland method. The detection of nitric oxide was
measured by a colorimetric assay and the inflammatory cytokines by flow cytometry.
We obtained particulate composites of calcium phosphates identified as
hydroxyapatite and silver nanoparticles. The bioactivity of the HA/Ag
nanocomposites on SFB was confirmed by apatite formations. The viability of MG-63
cells was not affected. We also found antimicrobial activity against Escherichia
coli, Staphylococcus aureus, and Candida albicans owing to the presence of silver
nanoparticles at non-cytotoxic concentrations. HA/Ag reduced the release of nitric
oxide and decreased the secretion of IL-1 and TNF-α in cells stimulated with
Lipopolysaccharide (LPS). In conclusion, the inflammatory and antimicrobial
capacity of the HA/Ag nanocomposites, as well as its bioactivity and low
cytotoxicity make it a candidate as an implantation biomaterial for bone tissues
engineering and clinical practices in orthopedic, oral and maxillofacial surgery. ©
The Author(s) 2019.
AN - rayyan-553780938
AU - Martínez-Sanmiguel, J. J.
AU - G Zarate-Triviño, D.
AU - Hernandez-Delgadillo, R.
AU - Giraldo-Betancur, A. L.
AU - Pineda-Aguilar, N.
AU - Galindo-Rodríguez, S. A.
AU - Franco-Molina, M. A.
AU - Hernández-Martínez, S. P.
AU - Rodríguez-Padilla, C.
DO - 10.1177/0885328219835995
IS - 10
KW - biocompatibility
biomaterial
composite materials
Nitric oxide
tissue engineering and bone regeneration
Bone Regeneration
Candida albicans
Candidiasis
Cell Line
Durapatite
Escherichia coli
Humans
Nanocomposites
Silver
Biocompatibility
Biomaterials
Bone
Calcium phosphate
Composite materials
Energy dispersive spectroscopy
Hydroxyapatite
Metal nanoparticles
Phosphate minerals
Photoelectron spectroscopy
Silver compounds
Surgery
Tissue
Tissue regeneration
calcium phosphate
hydroxyapatite
interleukin 1
lipopolysaccharide
nitric oxide
silver nanoparticle
antiinfective agent
nanocomposite
silver
Bone regeneration
Fourier transform infrared spectra
Inflammatory cytokines
Oral and maxillofacial surgeries
Osteoblast-like cells
Particulate composites
Release of nitric oxide
Article
cell viability
controlled study
cytokine release
cytotoxicity
MG-63 cell line
nonhuman
osteoblast
particle size
powder
priority journal
synthesis
bacterial infection
bone regeneration
candidiasis
cell line
chemistry
drug effect
human
ultrastructure
Bioactivity
PY - 2019
SP - 1314-1326
ST - Anti-inflammatory and antimicrobial activity of bioactive
hydroxyapatite/silver nanocomposites
T2 - Journal of Biomaterials Applications
TI - Anti-inflammatory and antimicrobial activity of bioactive
hydroxyapatite/silver nanocomposites
85066163675&doi=10.1177%2f0885328219835995&partnerID=40&md5=b57e1e95ad12e1db7234161
4b617f941
VL - 33
ID - 8947
ER -
TY - JOUR
AB - ABSTRACT BACKGROUND: Serotonin (5-HT) is present in the epithelial
enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons.
The 5-HT is involved in regulating motility, secretion, gut sensation, immune
system and inflammation. OBJECTIVE: Evaluate the effects of diabetes and quercetin
supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal
mucosa of streptozotocin-induced diabetic rats (STZ-rats). METHODS: Twenty-four
male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic
supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented
with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and
fixated in Zamboni's solution for 18 h. After obtaining cryosections,
immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of
5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and
crypts were performed. RESULTS: The diabetic condition displayed an increase of the
number of 5-HT-IR cells in villi and crypts, while decreased number of these cells
was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals,
an increased density of apoptotic cells in epithelial villi and crypts of the
jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed
in lamina propria. Possibly, quercetin supplementation slightly suppressed the
apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the
opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin
supplementation on healthy animals promoted few changes of serotoninergic function
and apoptotic stimuli. CONCLUSION: These results suggest that quercetin
supplementation mostly improved the serotonergic function affected by diabetes
maybe due to antioxidant and anti-inflammatory properties of quercetin. RESUMO
CONTEXTO: A serotonina (5-HT) está presente nas células epiteliais enterocromafins
(CE), nos mastócitos da lâmina própria e nos neurônios entéricos. A 5-HT está
envolvida na regulação da motilidade, secreção, nocepção intestinal, sistema
imunológico e inflamação. Objetivo: Avaliar os efeitos do diabetes e da
suplementação de quercetina sobre a função serotoninérgica e a perda celular por
apoptose na mucosa jejunal de ratos diabéticos induzidos por estreptozotocina
(ratos STZ). MÉTODOS: Vinte e quatro ratos Wistar machos foram divididos em quatro
grupos: normoglicêmico (C), normoglicêmico suplementado com quercetina 40 mg/dia
(Q), diabético (D) e diabético suplementado com quercetina 40 mg/dia (DQ). Após 120
dias, o jejuno foi coletado e fixado na solução de Zamboni por 18 horas. Após a
obtenção de cortes em criostato, a imuno-histoquímica foi realizada para marcar 5-
HT e caspase-3. A quantificação de células imunorreativas (IR) à 5-HT e caspase-3
foram realizadas na lâmina própria, vilosidades e criptas. RESULTADOS: A condição
diabética ocasionou um aumento do número de células 5-HT-IR nas vilosidades e
criptas, enquanto que na lâmina própria houve uma redução dessas células, no jejuno
de ratos STZ. Nos animais diabéticos, foi observada uma densidade aumentada de
células apoptóticas no epitélio do jejuno, tanto nas vilosidades quanto nas
criptas, por outro lado um número reduzido de células caspase-3-IR foi observado na
lâmina própria. Possivelmente, a suplementação de quercetina suprimiu ligeiramente
os fenômenos de apoptose no epitélio de vilosidades e criptas do jejuno de ratos
STZ, no entanto, o efeito oposto foi observado nas células 5-HT-IR da lâmina
própria. A suplementação com quercetina em animais saudáveis promoveu poucas
alterações na função serotoninérgica e nos estímulos apoptóticos. CONCLUSÃO: Estes
resultados sugerem que a suplementação de quercetina melhorou principalmente a
função serotoninérgica afetada pelo diabetes, talvez devido às propriedades
antioxidantes e anti-inflamatórias da quercetina.
AN - rayyan-553780940
AU - Martins-Perles, Juliana Vanessa Colombo
AU - Zignani, Isabela
AU - Souza, Sara Raquel Garcia de
AU - Frez, Flávia Cristina Vieira
AU - Bossolani, Gleison Daion Piovezana
AU - Zanoni, Jacqueline Nelisis
DO - 10.1590/s0004-2803.201900000-81
IS - 4
KW - Apoptose
Apoptosis
Diabetes mellitus
Intestinal mucosa
Mucosa intestinal
Quercetin
Quercetina
Ratos
Rats
Serotonin
Serotonina
Caspases
LA - en
PY - 2019
SN - 0004-2803
SP - 405-411
ST - A suplementação com quercetina previne mudanças em células imunorreativas à
serotonina e caspase-3 do jejuno de ratos diabéticos
T2 - Arq. gastroenterol
TI - A suplementação com quercetina previne mudanças em células imunorreativas à
serotonina e caspase-3 do jejuno de ratos diabéticos
28032019000400405
VL - 56
Y2 - 10 y3 - 1
ID - 8948
ER -
TY - JOUR
AB - In this contribution, the great potential of surface enhanced Raman
spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte
molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme
activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in
lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine
catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being
able to determine the TPMT activity before starting a treatment using 6-
mercaptopurine, an optimized dosage can be applied to each patient and serious
toxicity appearing within thiopurine treatment will be prevented.
AN - rayyan-553780942
AU - Marz, A.
AU - Monch, B.
AU - Rosch, P.
AU - Kiehntopf, M.
AU - Henkel, T.
AU - Popp, J.
DO - 10.1007/s00216-011-4811-z
IS - 9
KW - Methyltransferases
Erythrocytes
PY - 2011
SN - 1618-2642
SP - 2755-2761
ST - Detection of thiopurine methyltransferase activity in lysed red blood cells
by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)
T2 - ANALYTICAL AND BIOANALYTICAL CHEMISTRY
TI - Detection of thiopurine methyltransferase activity in lysed red blood cells
by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)
VL - 400
Y2 - 7
ID - 8950
ER -
TY - JOUR
AB - To elucidate the mechanism of neuronal death in Alzheimer's disease, we
investigated the effects of overexpression of wild-type Alzheimer amyloid precursor
protein (APP) on neuronal cells and glial cells in vivo. When an APP695-expressing
adenovirus was injected into the dorsal hippocampal region, a number of neurons in
remote areas were positively stained with anti-APP monoclonal antibody, and
underwent severe degeneration from 3 to 7 days after viral inoculation. Most
degenerating neurons were immunopositive with both APP and activated caspase-3, but
some neurons that expressed activated caspase-3 were not expressing APP from 7 to
14 days after virus injection. In the neighborhood of the degenerating neurons,
activated microglia/macrophages, which were identified by the phenotypic marker
C3bi receptor (CD11b/c; OX-42), were observed, and some of them appeared to
phagocytose the caspase-3-immunopositive degenerating neurons. In addition to
microglia/macrophages, infiltrating leukocytes expressing CD45 or CD4 were also
detected. These results suggest that the increased accumulation of APP induced not
only caspase-3-mediated death machinery, but also inflammatory responses including
microglial activation. These inflammatory responses might cause further
neurodegeneration through the alternative pathway that might activate the caspase-
3-mediated death machinery without APP expression.
AN - rayyan-553782224
AU - Masumura, M.
AU - Hata, R.
AU - Nishimura, I.
AU - Uetsuki, T.
AU - Sawada, T.
AU - Yoshikawa, K.
DO - 10.1016/s0169-328x(00)00163-7
IS - 2
J2 - Brain Res Mol Brain Res
KW - Adenoviridae/*genetics
Alzheimer Disease/immunology/metabolism
Amyloid beta-Protein Precursor/*genetics
Animals
Apoptosis/immunology
Benzimidazoles
Caspase 3
Caspases/*metabolism
Cell Count
Encephalitis/enzymology
Fluorescent Dyes
Gene Expression Regulation, Viral
*Genetic Vectors
Hippocampus/cytology/enzymology/*immunology
Macrophages/immunology
Male
Microglia/immunology
Nerve Degeneration/immunology/metabolism
Neurons/cytology/enzymology/immunology
Rats
Rats, Wistar
Caspases
LA - eng
N1 - BF Research Institute, c/o National Cardiovascular Center, 7-1, 5-Chome,
Fujishiro-dai, Suita, 565-0873, Osaka, Japan. masumura@silver.ocn.ne.jp
PY - 2000
SN - 0169-328X (Print)
SP - 219-27
ST - Caspase-3 activation and inflammatory responses in rat hippocampus inoculated
with a recombinant adenovirus expressing the Alzheimer amyloid precursor protein
T2 - Brain research. Molecular brain research
TI - Caspase-3 activation and inflammatory responses in rat hippocampus inoculated
with a recombinant adenovirus expressing the Alzheimer amyloid precursor protein
VL - 80
Y2 - 9 y3 - 15
ID - 10134
ER -
TY - JOUR
AB - An endothelial cell growth-suppressing factor (EGSF) was purified from the
serum-free conditioned medium of the mouse P388D1 culture in the presence of
carboxymethylated curdlan. The purified EGSF showed two bands corresponding to the
molecular masses of 55 and 63 kDa by silver staining on a SDS-polyacrylamide gel
under reducing conditions. This factor strongly suppressed the proliferation of
endothelial cells from bovine artery, human umbilical vein, and human dermal vas
capillare and this suppression was observed to be reversible. We found that EGSF
was a potent chemoattractant for macrophages and neutrophils. EGSF mediated the
adhesion of neutrophils to BAEs and transendothelial migration of neutrophils.
Macrophages stimulated by EGSF produced nitrite in a dose-dependent manner. EGSF
did not affect the proliferation of T lymphocytes. These findings suggest that EGSF
acts not only as a potent inhibitor for the growth of endothelial cells but also an
activator for macrophages and neutrophils. Thus EGSF plays a role in an
inflammatory response in the endothelium.
AN - rayyan-553780943
AU - Matsunaga, T.
AU - Usui, S.
AU - Ukai, S.
AU - Kiho, T.
AU - Hirano, K.
DO - 10.1271/bbb.63.1228
IS - 7
KW - Macrophages
Macrophage Activation
PY - 1999
SN - 0916-8451 1347-6947
SP - 1228-1237
ST - Activation of macrophages and neutrophils by an endothelium growth
suppressing factor
T2 - BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
TI - Activation of macrophages and neutrophils by an endothelium growth
suppressing factor
VL - 63
Y2 - 7
ID - 8951
ER -
TY - JOUR
AB - INTRODUCTION: Administration of the NO inhibitor Nwð-nitro-L-arginine methyl
ester (NAME) and a high-salt diet (HS) promotes severe albuminuria and renal
injury, which regresses upon discontinuation of treatments. OBJECTIVE: We
investigated whether these changes reappear after reinstitution of HS, and whether
they are prevented by treatment with the antilymphocyte agent mycophenolate mofetil
(MMF) or the AT-1 receptor blocker losartan (L). Adult male Munich-Wistar rats
received NAME and HS. A control Group (C) received only HS. After 20 days, rats
receiving HS and NAME exhibited severe hypertension and albuminuria. After a 30-day
recovery period, hypertension was attenuated and albuminuria had virtually
disappeared. MATERIAL AND METHODS: Rats were then distributed among the following
groups: HS, receiving HS; NS, receiving a normal salt (NS) diet; HS-MMF, receiving
HS and MMF; HS-LOS, receiving HS and L; HS-HDZ, receiving HS and hydralazine (HDZ).
Sixty days later, NS rats showed only slight albuminuria and renal damage or
inflammation. In contrast, HS rats developed severe hypertension, marked
glomerulosclerosis with interstitial expansion and renal infiltration by
macrophages and angiotensin II-positive cells. The group treated with losartan had
lowered blood pressure and a lack of albuminuria or renal injury. MMF provided
similar protection without altering blood pressure, suggesting a nonhemodynamic
effect, a hypothesis reinforced by the finding that HDZ lowered blood pressure
without preventing renal injury. RESULTS: These results indicate that treatment
with HS and NAME predisposes to the development of hypertension and renal injury
upon salt overload, characterizing a new model of chronic nephropathy. CONCLUSION:
The response to MMF or L, but not HDZ, suggests a key role for inflammatory rather
than hemodynamic factors. INTRODUÇÃO: A administração de Nômega-nitro-L-arginina
metiléster (NAME), um inibidor da produção de NO, com dieta rica em sal (HS)
promove albuminúria e dano renal graves, reversíveis ao interromperem-se os
tratamentos. OBJETIVO: Investigamos se tais alterações recrudescem ao reinstituir-
se a HS e se são prevenidas pelo micofenolato mofetil (MMF), um agente
antilinfócito, ou losartan, um bloqueador do receptor AT-1. MATERIAL E MÉTODOS:
Ratos Münich-Wistar machos adultos receberam NAME e HS. Um grupo controle (C)
recebeu apenas HS. Após 20 dias, os ratos que receberam HS e NAME exibiam
hipertensão e albuminúria graves. Após recuperação de 30 dias, a hipertensão
atenuou-se e a albuminúria praticamente desapareceu. Formaram-se então os grupos:
HS, recebendo HS; NS, recebendo dieta normal em sal (NS); HS-MMF, recebendo HS e
MMF; HS-LOS, recebendo HS e losartan; HS-HDZ, recebendo HS e hidralazina. Após
sessenta dias os ratos NS tinham albuminúria e dano/inflamação renal apenas
discretos. Já os ratos HS desenvolveram hipertensão e glomerulosclerose acentuadas,
expansão intersticial e infiltração renal por macrófagos e células positivas para
angiotensina II. Losartan baixou a pressão arterial e preveniu albuminúria e lesão
renal. MMF proporcionou proteção semelhante sem alteração pressórica, sugerindo a
ação de mecanismos não hemodinâmicos, hipótese reforçada pelo achado de que a HDZ
baixou a pressão arterial sem prevenir a nefropatia. RESULTADOS: Esses resultados
indicam que o tratamento com HS e NAME predispõe ao desenvolvimento de hipertensão
e lesão renal induzidos por excesso de sal, caracterizando um novo modelo de
nefropatia crônica. CONCLUSÃO: A resposta ao MMF ou losartan, mas não à
hidralazina, sugere o predomínio de fatores inflamatórios.
AN - rayyan-553780944
AU - Mattar, Ana Lúcia
AU - Machado, Flávia Gomes
AU - Fujihara, Clarice Kazue
AU - Malheiros, Denise Maria Avancini Costa
AU - Zatz, Roberto
IS - 6
KW - AT-1 receptor blocker
AT-1 receptor bloqueador
Angiotensin II
Angiotensina
Chronic kidney disease
Doença crônica do rim
Excesso de sal
Micofenolato mofetil
Mycophenolate mofetil
Salt overload
Nitric Oxide
LA - en
PY - 2007
SN - 1807-5932
SP - 749-756
ST - Persistent hypertension and progressive renal injury induced by salt overload
after short term nitric oxide inhibition
T2 - Clinics
TI - Persistent hypertension and progressive renal injury induced by salt overload
after short term nitric oxide inhibition
59322007000600015
VL - 62
ID - 8952
ER -
TY - JOUR
AB - Visceral leishmaniasis (VL) is caused by the protozoan parasite Leishmania
donovani There are no vaccines and available drugs against leishmaniasis are toxic.
Immunomodulators that specifically boost the anti-microbial activities of the
immune cells could alleviate several of these limitations. Therefore, finding novel
immunomodulators for VL therapy is a pressing need. This study is aimed to evaluate
the immunomodulatory role of leptin, an adipocyte-derived hormone capable of
regulating the immune response, in L. donovani-infected mice. We observed that
recombinant leptin treatment reduced splenic parasite burden compared with non-
treated infected normal mice. Decrease in parasite burden correlated with an
induction of innate immune response in antigen-presenting cells that showed an
increase in nitric oxide, enhanced pro-inflammatory cytokine (interferon gamma
[IFNγ], interleukin12 [IL]12, and IL1β) response in the splenocytes, indicating
host-protecting Th1 response mediated by leptin. Moreover, in infected normal mice,
leptin treatment induced IFNγ production from both CD4(+) and CD8(+) T cells,
compared with non-treated infected mice. Alternatively, leptin-deficient (Ob/Ob)
mice had higher splenic and liver parasite burden compared with the infected normal
mice. However, leptin treatment failed to reduce the splenic parasite burden and
improve a host-protective cytokine response in these mice. In addition, in contrast
to dendritic cells (DCs) from a normal mouse, Ob/Ob mouse-derived DCs showed a
defect in the induction of innate immune response on Leishmania infection that
could not be reversed by leptin treatment. Therefore, our findings reveal that
leptin has a differential immunomodulatory effect in controlling VL in normal and
Ob/Ob mice.
AN - rayyan-553782222
AU - Maurya, R.
AU - Ismail, N.
AU - Dagur, P. K.
AU - Joshi, A. B.
AU - Razdan, K.
AU - McCoy, J. P.
AU - Ascher, J.
AU - Dey, R.
AU - Nakhasi, H. L.
DO - 10.4269/ajtmh.15-0804
IS - 1
J2 - Am J Trop Med Hyg
KW - Animals
CD4-Positive T-Lymphocytes/immunology
CD8-Positive T-Lymphocytes/immunology
Female
Immunity, Innate
Immunologic Factors/*pharmacology
Interferon-gamma/immunology
Interleukin-12/immunology
Interleukin-1beta/immunology
Leishmania donovani/drug effects
Leishmaniasis, Visceral/blood/*immunology
Leptin/blood/deficiency/*pharmacology
Mice
Mice, Inbred C57BL
Mice, Obese
Recombinant Proteins/pharmacology
Spleen/drug effects/immunology/parasitology
Leptin
LA - eng
N1 - Department of Animal Biology, School of Life Science, University of
Hyderabad, Hyderabad, India.; Division of Emerging and Transfusion Transmitted
Diseases, Center for Biologics Evaluation and Research, Food and Drug
Administration, Silver Spring, Maryland.; Division of Emerging and Transfusion
Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug
Administration, Silver Spring, Maryland.; Division of Emerging and Transfusion
Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug
Administration, Silver Spring, Maryland.; Flow Cytometry Core, National Heart,
Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland.;
Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics
Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland.;
Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics
Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland.;
Flow Cytometry Core, National Heart, Lung, and Blood Institute, National Institutes
of Health, Bethesda, Maryland.; Division of Veterinary Services, Center for
Maryland.; Division of Emerging and Transfusion Transmitted Diseases, Center for
Maryland.; Division of Emerging and Transfusion Transmitted Diseases, Center for
Maryland.
PY - 2016
SP - 109-119
ST - Differential Role of Leptin as an Immunomodulator in Controlling Visceral
Leishmaniasis in Normal and Leptin-Deficient Mice
T2 - The American journal of tropical medicine and hygiene
TI - Differential Role of Leptin as an Immunomodulator in Controlling Visceral
Leishmaniasis in Normal and Leptin-Deficient Mice
VL - 95
Y2 - 7 y3 - 6
ID - 10132
ER -
TY - JOUR
AB - The skin, acting as the outer protection of the human body, is most
vulnerable to injury. Wound healing can often be impaired, leading to chronic,
hard-to-heal wounds. For this reason, searching for the most effective dressings
that can significantly enhance the wound healing process is necessary. In this
regard, silk fibroin, a protein derived from silk fibres that has excellent
properties, is noteworthy. Silk fibroin is highly biocompatible and biodegradable.
It can easily make various dressings, which can be loaded with additional
substances to improve healing. Dressings based on silk fibroin have anti-
inflammatory, pro-angiogenic properties and significantly accelerate skin wound
healing, even compared to commercially available wound dressings. Animal studies
confirm the beneficial influence of silk fibroin in wound healing. Clinical
research focusing on fibroin dressings is also promising. These properties make
silk fibroin a remarkable natural material for creating innovative, simple, and
effective dressings for skin wound healing. In this review, we summarise the
application of silk fibroin biomaterials as wound dressings in full-thickness,
burn, and diabetic wounds in preclinical and clinical settings. © 2022 by the
authors.
AN - rayyan-553780945
AU - Mazurek, Ł
AU - Szudzik, M.
AU - Rybka, M.
AU - Konop, M.
DO - 10.3390/biom12121852
IS - 12
KW - burn wounds
diabetic wounds
silk biomaterials
silk fibroin
skin wounds
wound healing
biomaterial
colony stimulating factor 1
hydrogel
protein kinase B
silver nanoparticle
somatomedin C
biocompatibility
burn
cell proliferation
diabetes mellitus
diabetic wound
epithelization
histology
human
neutrophil
nonhuman
preclinical study
Review
skin injury
systematic review
thickness
Wound Healing
Silk
Skin
PY - 2022
ST - Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing
T2 - Biomolecules
TI - Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing
85144692159&doi=10.3390%2fbiom12121852&partnerID=40&md5=077a060394c1ca0642004526f2f
488ed
VL - 12
ID - 8953
ER -
TY - JOUR
AB - The objective of this study was to determine the effect of a nonionic silver
nanocolloid administered orally for 7 or 14 days at three concentration levels (25
ppm, 2.5 ppm, and 0.25 ppm) on the phagocytic activity and mitogenic response of
splenocytes and selected cytokine serum levels (IL-1beta, IL-6, IL-10, IL-12 p70,
TNF-alpha) in NMRI mice at the early stage of experimental endotoxemia induced with
single 30 microg/mouse dose of bacterial LPS. Regardless of the period of
administration, silver nanoparticles enhanced the production of proinflammatory
cytokines and anti-inflammatory cytokine IL-10, and they inhibited IL-12 p70 levels
in response to LPS challenge. The studied nanoparticles' effect on splenocyte
activity was determined by the period of administration. After 7 days of use,
silver nanoparticles enhanced the phagocytic activity, and doses of 2.5 ppm
stimulated the mitogenic response of splenocytes. After 14 days of administration,
silver nanoparticles lowered the phagocytic activity regardless of the dose
applied. Although the results obtained are ambiguous, they suggest that silver
nanoparticles administered via the alimentary tract are more likely to increase an
inflammatory response of an organism than offer protection after LPS challenge.
AN - rayyan-553782018
AU - Małaczewska, J.
DO - 10.2478/v10181-011-0089-5
IS - 4
J2 - Pol J Vet Sci
KW - Animals
Cytokines/*blood/metabolism
Drug Administration Schedule
Endotoxemia/chemically induced/*drug therapy
Male
Metal Nanoparticles/administration & dosage/chemistry/*therapeutic use
Mice
Silver/administration & dosage/chemistry/*therapeutic use
Spleen/*cytology/drug effects
Cytokines
LA - eng
N1 - Department of Microbiology and Clinical Immunology, Faculty of Veterinary
Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn,
Poland. j.malaczewska7@wp.pl
PY - 2011
SN - 1505-1773 (Print)
SP - 597-604
ST - The effect of silver nanoparticles on splenocyte activity and selected
cytokine levels in the mouse serum at early stage of experimental endotoxemia
T2 - Polish journal of veterinary sciences
TI - The effect of silver nanoparticles on splenocyte activity and selected
cytokine levels in the mouse serum at early stage of experimental endotoxemia
VL - 14
ID - 9934
ER -
TY - COMP
AB - OBJECTIVE: To explore the transcriptome of epicardial adipose tissue (EAT) as
compared to subcutaneous adipose tissue (SAT) and its modifications in a small
number of patients with coronary artery disease (CAD) versus valvulopathy. METHODS:
SAT and EAT samples were obtained during elective cardiothoracic surgeries. The
transcriptome of EAT was evaluated, as compared to SAT, using an unbiased, whole-
genome approach in subjects with CAD (n = 6) and without CAD (n = 5), where the
patients without CAD had cardiac valvulopathy. RESULTS: Relative to SAT, EAT is a
highly inflammatory tissue enriched with genes involved in endothelial function,
coagulation, immune signaling, potassium transport, and apoptosis. EAT is lacking
in expression of genes involved in protein metabolism, tranforming growth factor-
beta (TGF-beta) signaling, and oxidative stress. Although underpowered, in subjects
with severe CAD, there is an expression trend suggesting widespread downregulation
of EAT encompassing a diverse group of gene sets related to intracellular
trafficking, proliferation/transcription regulation, protein catabolism, innate
immunity/lectin pathway, and ER stress. CONCLUSIONS: The EAT transcriptome is
unique when compared to SAT. In the setting of CAD versus valvulopathy, there is
possible alteration of the EAT transcriptome with gene suppression. This pilot
study explores the transcriptome of EAT in CAD and valvulopathy, providing new
insight into its physiologic and pathophysiologic roles.
AN - rayyan-553782152
AU - McAninch, E. A.
AU - Fonseca, T. L.
AU - Poggioli, R.
AU - Panos, A. L.
AU - Salerno, T. A.
AU - Deng, Y.
AU - Li, Y.
AU - Bianco, A. C.
AU - Iacobellis, G.
CY - United States
DO - 10.1002/oby.21059
ET - 6
KW - Adipose Tissue/*metabolism
Coronary Artery Disease/genetics/*metabolism
Female
Humans
Male
Pericardium/*metabolism
Pilot Projects
*Transcriptome
Transcriptome
Adipose Tissue
Tocopherols
LA - eng
N1 - Department of Medicine, Division of Endocrinology and Metabolism, Rush
University Medical Center, Chicago, Illinois, USA.; Department of Medicine,
Division of Endocrinology and Metabolism, Rush University Medical Center, Chicago,
Illinois, USA.; Department of Medicine, Division of Endocrinology, Diabetes and
Metabolism, University of Miami Miller School of Medicine, Miami, Florida, USA.;
Department of Surgery, Division of Thoracic and Cardiac Surgery, University of
Miami Miller School of Medicine, Miami, Florida, USA.; Department of Surgery,
Division of Thoracic and Cardiac Surgery, University of Miami Miller School of
Medicine, Miami, Florida, USA.; Department of Medicine, Rush University Medical
Center, Chicago, Illinois, USA.; Department of Medicine, Rush University Medical
Center, Chicago, Illinois, USA.; Department of Medicine, Division of Endocrinology
and Metabolism, Rush University Medical Center, Chicago, Illinois, USA.; Department
of Medicine, Division of Endocrinology, Diabetes and Metabolism, University of
Miami Miller School of Medicine, Miami, Florida, USA.
PY - 2015
SP - 1267-78
ST - Epicardial adipose tissue has a unique transcriptome modified in severe
coronary artery disease
TI - Epicardial adipose tissue has a unique transcriptome modified in severe
coronary artery disease
VL - 23
Y2 - 6
ID - 10063
ER -
TY - JOUR
AB - During the 1991 GulfWar, U.S. service members were exposed to depleted
uranium (DU) through friendly-fire incidents involving DU munitions and vehicles
protected by DU armor. Routes of exposure to DU involved inhalation of soluble and
insoluble DU oxide particles, wound contamination, and retained embedded DU metal
fragments that continue to oxidize in situ and release DU to the systemic
circulation. A biennial health surveillance program established for this group of
Veterans by the U. S. Department of Veterans Affairs has shown continuously
elevated urine DU concentrations in the subset of veterans with embedded fragments
for over 20 years. While the 2011 assessment was comprehensive, few clinically
significant U-related health effects were observed. This report is focused on
health outcomes associated with two primary target organs of concern for long term
effects of this combat-related exposure to DU. Renal biomarkers showed minimal DU-
related effects on proximal tubule function and cytotoxicity, but significant
biomarker results were observed when urine concentrations of multiple metals also
found in fragments were examined together. Pulmonary tests and questionnaire
results indicate that pulmonary function after 20 y remains within the clinical
normal range. Imaging of DU embedded fragment-associated tissue for signs of
inflammatory or proliferative reactions possibly associated with foreign body
transformation or with local alpha emissions from DU was also conducted using PET-
CT and ultrasound. These imaging tools may be helpful in guiding decisions
regarding removal of fragments. Health Phys. 104(4):347Y361; 2013
AN - rayyan-553780948
AU - McDiarmid, M. A.
AU - Gaitens, J. M.
AU - Hines, S.
AU - Breyer, R.
AU - Wong-You-Cheong, J. J.
AU - Engelhardt, S. M.
AU - Oliver, M.
AU - Gucer, P.
AU - Kane, R.
AU - Cernich, A.
AU - Kaup, B.
AU - Hoover, D.
AU - Gaspari, A. A.
AU - Liu, J.
AU - Harberts, E.
AU - Brown, L.
AU - Centeno, J. A.
AU - Gray, P. J.
AU - Xu, H. N.
AU - Squibb, K. S.
DO - 10.1097/HP.0b013e31827b1740
IS - 4
KW - Biological Assay
War
PY - 2013
SN - 0017-9078 1538-5159
SP - 347-361
ST - THE GULF WAR DEPLETED URANIUM COHORT AT 20 YEARS: BIOASSAY RESULTS AND NOVEL
APPROACHES TO FRAGMENT SURVEILLANCE
T2 - HEALTH PHYSICS
TI - THE GULF WAR DEPLETED URANIUM COHORT AT 20 YEARS: BIOASSAY RESULTS AND NOVEL
APPROACHES TO FRAGMENT SURVEILLANCE
VL - 104
Y2 - 4
ID - 8956
ER -
TY - JOUR
AB - Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been
associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-
thickness human-derived oral mucosa model employed for biocompatibility testing of
base-metal dental alloys would provide insights into the mechanisms of Ni-induced
toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto
Alloderm™ and maintained until full thickness was achieved prior to Ni-Cr and
cobalt-chromium (Co-Cr) alloy disc exposure (2-72 h). Biocompatibility assessment
involved histological analyses with cell viability measurements, oxidative stress
responses, inflammatory cytokine expression and cellular toxicity analyses.
Inductively coupled plasma mass spectrometry analysis determined elemental ion
release levels. We detected adverse morphology with significant reductions in cell
viability, significant increases in oxidative stress, inflammatory cytokine
expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models
compared with untreated oral mucosal models, and adverse effects were increased for
the Ni-Cr alloy that leached the most Ni. Co-Cr demonstrated significantly enhanced
biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-
derived full-thickness oral mucosal model discriminated between dental alloys and
provided insights into the mechanisms of Ni-induced toxicity, highlighting
potential clinical relevance. © 2011 Acta Materialia Inc. Published by Elsevier
Ltd. All rights reserved.
AN - rayyan-553780949
AU - McGinley, E. L.
AU - Moran, G. P.
AU - Fleming, G. J. P.
DO - 10.1016/j.actbio.2011.08.017
IS - 1
Dental casting alloys
ICP-MS
Inflammation
Oxidative stress
Binary alloys
Cell culture
Cobalt alloys
Cytotoxicity
Dental alloys
Inductively coupled plasma mass spectrometry
Nickel alloys
Silver alloys
chromium
cobalt
cytokine
nickel
Base metals
Biocompatibility testing
Cellular toxicities
Cytokine expression
Fixed prosthodontics
Keratinocytes
Nickel-Chromium alloys
Oral mucosa
article
bare metal stent
biocompatibility
cell viability
controlled study
cytotoxicity
denture
fibroblast
histology
human
human tissue
keratinocyte
mass spectrometry
mouth mucosa
normal human
oxidative stress
priority journal
protein expression
semen analysis
Chromium alloys
Humanities
Humanism
Humans
Stomatitis
PY - 2012
SP - 432-438
ST - Base-metal dental casting alloy biocompatibility assessment using a human-
derived three-dimensional oral mucosal model
T2 - Acta Biomaterialia
TI - Base-metal dental casting alloy biocompatibility assessment using a human-
derived three-dimensional oral mucosal model
80255130971&doi=10.1016%2fj.actbio.2011.08.017&partnerID=40&md5=a1d1ec499eecb7031b1
a199308b4e9a4
VL - 8
ID - 8957
ER -
TY - JOUR
AB - History shows that metal-based drugs and remedies have been known and used
since very ancient times. For example, silver was employed in the treatment of
wounds and ulcers according to the Greek physician Hippocrates, but its
antimicrobial properties had probably been recognized long before because was used
to make vessels for storing liquids in pure form. The ancient Egyptians also knew
how to sterilize water with copper. The medical use of gold can be dated back to
2500 B.C. in China. However, the new era of metal-based medicine started almost 50
years ago when cisplatin was shown to inhibit cellular division in Escherichia
coli, thereby leading to the first studies of its antitumor activity in rats and
its assessment as one of the most powerful drugs for use against different types of
cancer, although many other novel metal-based drugs are promising and they are
attracting growing attention in modern clinical medicine. Gold salts and arsenic
compounds have been in use for decades in the treatment of rheumatoid arthritis and
syphilis, respectively, but studies of cisplatin have definitely shifted the
attention of researchers to the pool of transition "heavy" metals as potential
therapeutic agents. Rhodium, iridium, palladium, osmium, and the other so-called
noble elements have been the subjects of intensive investigations, thereby leading
to the production of a series of complex compounds with remarkable anticancer
activities, as well as antirheumatic, antimalarial, and antimicrobial drugs. The
number of published studies in this field is huge and they have already been the
subjects of careful review. In this review, we provide a detailed account of the
latest results (2010-2013) and their potential uses in the cure of severe diseases.
AN - rayyan-553780950
AU - Medici, S.
AU - Peana, M.
AU - Nurchi, V. M.
AU - Lachowicz, J. I.
AU - Crisponi, G.
AU - Zoroddu, M. A.
DO - 10.1016/j.ccr.2014.08.002
KW - Metals
PY - 2015
SN - 0010-8545 1873-3840
SP - 329-350
ST - Noble metals in medicine: Latest advances
T2 - COORDINATION CHEMISTRY REVIEWS
TI - Noble metals in medicine: Latest advances
VL - 284
Y2 - 2 y3 - 1
ID - 8958
ER -
TY - JOUR
AB - Silver has no biological role, and it is particularly toxic to lower
organisms. Although several silver formulations employed in medicine in the past
century are prescribed and sold to treat certain medical conditions, most of the
compounds, including those showing outstanding properties as antimicrobial or
anticancer agents, are still in early stages of assessment, that is, in vitro
studies, and may not make it to clinical trials. Unlike other heavy metals, there
is no evidence that silver is a cumulative poison, but its levels can build up in
the body tissues after prolonged exposure leading to undesired effects. In this
review, we deal with the journey of silver in medicine going from the alternative
or do-it-yourself drug to scientific evidence related to its uses. The many
controversies push scientists to move toward a more comprehensive understanding of
the mechanisms involved.
AN - rayyan-553781780
AU - Medici, S.
AU - Peana, M.
AU - Nurchi, V. M.
AU - Zoroddu, M. A.
DO - 10.1021/acs.jmedchem.8b01439
IS - 13
J2 - J Med Chem
KW - Animals
Anti-Infective Agents/pharmacology/therapeutic use/toxicity
Anti-Inflammatory Agents/pharmacology/therapeutic use/toxicity
Antineoplastic Agents/pharmacology/therapeutic use/toxicity
Coordination Complexes/pharmacology/therapeutic use/toxicity
Fungi/drug effects
Humans
Metal Nanoparticles/chemistry/therapeutic use/toxicity
Silver/*pharmacology/*therapeutic use/toxicity
Viruses/drug effects
LA - eng
N1 - Department of Chemistry and Pharmacy , University of Sassari , 07100
Sassari , Italy.; Department of Chemistry and Pharmacy , University of Sassari ,
07100 Sassari , Italy.; Department of Life and Environmental Sciences , University
of Cagliari , 09042 Cagliari , Italy.; Department of Chemistry and Pharmacy ,
University of Sassari , 07100 Sassari , Italy.
PY - 2019
SP - 5923-5943
ST - Medical Uses of Silver: History, Myths, and Scientific Evidence
T2 - Journal of medicinal chemistry
TI - Medical Uses of Silver: History, Myths, and Scientific Evidence
VL - 62
Y2 - 7 y3 - 11
ID - 9711
ER -
TY - JOUR
AB - Heavy metals have different adverse impacts on different life stages of fish
species with attempts to use natural antioxidants to counteract their effects. So,
the present study investigated the potential protective effects of Amphora
coffeaeformis extract against arsenic-induced hemato-biochemical alterations in
African catfish, Clarias gariepinus. The fish exposed to sub-lethal concentrations
of arsenic; 19.2 and 38.3 mg/L (1/8 and 1/4 of 96h-LC50 value, 153.17 mg/L) for 15
days. The main effect of arsenic was recorded in some blood parameters such as
RBC's count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and
white blood cells. As for biochemical parameters, the main effect of arsenic was
significant for alkaline phosphatase, glucose, uric acid, creatinine, albumin,
globulin, and albumin/globulin. Also, the residue of arsenic in fish muscles showed
significant effects. The majority of these arsenic-induced parameters were improved
with dietary supplements of the diatom A. coffeaeformis. So, Amphora extract can be
used as detoxification factor on fishes induced by arsenic due to its biologically
active components providing protections like antioxidant, antiviral, antibacterial,
and anti-inflammatory. Besides, they have excellent contents of proteins and
carbohydrates which are supposed to enhance the effect of these compounds.
AN - rayyan-553780953
AU - Mekkawy, I. A.
AU - Mahmoud, U. M.
AU - Moneeb, R. H.
AU - Sayed, Aedh
DO - 10.3389/fmars.2020.00191
PY - 2020
SN - 2296-7745
ST - Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato-
Biochemical Alterations of African Catfish (Clarias gariepinus)
T2 - FRONTIERS IN MARINE SCIENCE
TI - Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato-
Biochemical Alterations of African Catfish (Clarias gariepinus)
VL - 7
Y2 - 3 y3 - 31
ID - 8961
ER -
TY - JOUR
AB - Metal and metal oxide nanoparticles (NPs) are promising antibacterial agents.
They have a broad antimicrobial activity against both Gram-positive and Gram-
negative bacteria, viruses, and protozoans. The use of NPs reduces the possibility
of the microbial resistance development. This review briefly shows the general
mechanisms and the main factors of antibacterial activity of NPs. In this article,
a comprehensive review of the recent researches in the field of new antimicrobial
agents with superior long-term bactericidal activity and low toxicity is provided.
The review gives the examples of synthesis of double and triple nanocomposites
based on following oxides: CuO, ZnO, Fe3O4, Ag2O, MnO2, etc. including metal and
nonmetal doped nanocomposites (for example with Ag, Ce, Cr, Mn, Nd, Co, Sn, Fe, N,
F, etc.). Compared with bactericidal action of individual oxides, the
nanocomposites demonstrate superior antibacterial activity and have synergistic
effects. For example, the antimicrobial activity of ZnO against both Gram-positive
and Gram-negative bacteria was increased by -100% by formation of triple
nanocomposites ZnO-MnO2-Cu2O or ZnO-Ag2O-Ag2S. Similar effect was showed for Ce-
doped ZnO and Zn-doped CuO. The present article also provides the examples of
nanocomposites containing NPs and organic (chitosan, cellulose,
polyvinylpyrrolidone, biopolymers, etc.) or inorganic materials with special
structure (graphene oxide, TiO2 nanotubes, silica) which demonstrate controlled
release and longterm antibacterial activity. All of the considered nanocomposites
and their combinations have a pronounced long-term antimicrobial effect including
against antibiotic-resistant strains. They are able to prevent the formation of
microbial biofilms on biotic and abiotic surfaces, have low toxicity to eukaryotic
cells, demonstrate anti-inflammatory and woundhealing properties in compositions
with polymers (sodium alginate, collagen, polyvinylpyrrolidone, etc.). The use of
nanoscale systems can solve several important practical problems at the same time:
saving of long-term antimicrobial activities while reducing the number of
compounds, creation of new antimicrobial agents with low toxicity and reduced
environmental impact, development of new biocidal materials, including new coatings
for effective antimicrobial protection of medical devices.
AN - rayyan-553780954
AU - Meleshko, A. A.
AU - Afinogenova, A. G.
AU - Afinogenov, G. E.
AU - Spiridonova, A. A.
AU - Tolstoy, V. P.
DO - 10.15789/2220-7619-AIA-1512
IS - 4
PY - 2020
SN - 2220-7619 2313-7398
SP - 639-654
ST - ANTIBACTERIAL INORGANIC AGENTS: EFFICIENCY OF USING MULTICOMPONENT SYSTEMS
T2 - INFEKTSIYA I IMMUNITET
TI - ANTIBACTERIAL INORGANIC AGENTS: EFFICIENCY OF USING MULTICOMPONENT SYSTEMS
VL - 10
Y2 - 10
ID - 8962
ER -
TY - JOUR
AB - The secretome of human amniotic fluid stem cells (AFSCs) has great potential
as a therapeutic agent in regenerative medicine. However, it must be produced in a
clinically compliant manner before it can be used in humans. In this study, we
developed a means of producing a biologically active secretome from AFSCs that is
free of all exogenous molecules. We demonstrate that the full secretome is capable
of promoting stem cell proliferation, migration, and protection of cells against
senescence. Furthermore, it has significant anti-inflammatory properties. Most
importantly, we show that it promotes tissue regeneration in a model of muscle
damage. We then demonstrate that the secretome contains extracellular vesicles
(EVs) that harbor much, but not all, of the biological activity of the whole
secretome. Proteomic characterization of the EV and free secretome fraction shows
the presence of numerous molecules specific to each fraction that could be key
regulators of tissue regeneration. Intriguingly, we show that the EVs only contain
miRNA and not mRNA. This suggests that tissue regeneration in the host is mediated
by the action of EVs modifying existing, rather than imposing new, signaling
pathways. The EVs harbor significant anti-inflammatory activity as well as promote
angiogenesis, the latter may be the mechanistic explanation for their ability to
promote muscle regeneration after cardiotoxin injury. © Copyright 2017, Mary Ann
Liebert, Inc. 2017.
AN - rayyan-553780955
AU - Mellows, B.
AU - Mitchell, R.
AU - Antonioli, M.
AU - Kretz, O.
AU - Chambers, D.
AU - Zeuner, M. T.
AU - Denecke, B.
AU - Musante, L.
AU - Ramachandra, D. L.
AU - Debacq-Chainiaux, F.
AU - Holthofer, H.
AU - Joch, B.
AU - Ray, S.
AU - Widera, D.
AU - David, A. L.
AU - Huber, T. B.
AU - Dengjel, J.
AU - De Coppi, P.
AU - Patel, K.
DO - 10.1089/scd.2017.0089
IS - 18
KW - miRNA
muscle
regeneration
secretome
Amniotic Fluid
Animals
Cell Line
Cells, Cultured
Embryonic Stem Cells
Extracellular Vesicles
Humans
Male
Mice
Mice, Inbred C57BL
MicroRNAs
Muscle, Skeletal
Proteome
Regeneration
ABCE1 protein
ABCF1 protein
ABR protein
AP2A1 protein
beta galactosidase
C73 protein
CD34 antigen
CD45 antigen
CYFIP1 protein
CYFIP2 protein
DNM1 protein
DNM2 protein
DNM3 protein
heat shock protein
Hermes antigen
hexokinase 1
histone deacetylase 1
histone deacetylase 2
luciferase
microRNA
microRNA 125b 5p
microRNA 1273 3p
microRNA 3196
microRNA 762
myosin heavy chain
nucleic acid
protein
Thy 1 antigen
unclassified drug
proteome
acute disease
adipogenesis
amniotic fluid stem cell
angiogenesis
animal experiment
animal model
apoptosis
Article
autophagy
biological activity
bone development
capillary density
cell count
cell function
cell migration
cell proliferation
cell protection
cell stress
chondrogenesis
controlled study
enzyme activation
enzyme activity
exosome
female
human
human cell
in vivo study
male
mouse
muscle injury
muscle regeneration
nonhuman
priority journal
senescence
signal transduction
silver staining
amnion fluid
animal
C57BL mouse
cell culture
cell line
cytology
embryonic stem cell
genetics
metabolism
physiology
skeletal muscle
transplantation
PY - 2017
SP - 1316-1333
ST - Protein and Molecular Characterization of a Clinically Compliant Amniotic
Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating
Muscle Regeneration Through Enhancement of Angiogenesis
T2 - Stem Cells and Development
TI - Protein and Molecular Characterization of a Clinically Compliant Amniotic
Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating
Muscle Regeneration Through Enhancement of Angiogenesis
85029223185&doi=10.1089%2fscd.2017.0089&partnerID=40&md5=41235926ea1391cb02b6f6f148
c57ed2
VL - 26
ID - 8963
ER -
TY - CONF
AB - The aim of this study was to assess whether thermal trauma induced oxidative
stress altered the balance between oxidant and antioxidant systems in the blood of
burn wound rats in the absence and presence of silver nanoparticles and S-
nitrosoglutathione, GSNO. Free silver nanoparticles, free GSNO and silver
nanoparticles + GSNO had no cytotoxic effects. Under anesthesia, the shaved dorsum
of the rats was exposed to 90°C (burn group) water bath. Studied compounds were
administered topically immediately and at 28 days after the burn injury, four times
a day. Silver nanoparticles and silver nanoparticles + GSNO were no toxic in vitro
and in vivo. There were no significant differences in the levels of urea,
creatinine, aminotransferases and hematological parameters, in control-burn groups
(free silver nanoparticles) and treated-burn groups (free GSNO or silver
nanoparticles + GSNO). There were no differences in lipid peroxidation and in the
levels of protein carbonyls and glutathione, used as oxidative stress markers. A
little inflammatory cell response, papillary dermis vascularization, fibroblasts
differentiated into contractile myofibroblasts and the presence of a large amount
of extracellular matrix were evidenced in treated groups following skin injury.
These results indicate that silver nanoparticles and GSNO may provide an effective
action on wound healing.
AN - rayyan-553780956
AU - Melo, P. S.
AU - Marcato, P. D.
AU - Huber, S. C.
AU - Ferreira, I. R.
AU - Paula, L. B. De
AU - Almeida, A. B. A.
AU - Durán, N.
AU - Torsoni, S.
AU - Seabra, A. B.
AU - Alves, O. L.
DA - 2011
DO - 10.1088/1742-6596/304/1/012027
KW - Cell culture
Cytotoxicity
Fibroblasts
Metal nanoparticles
Nanoparticles
Oxidative stress
Rats
Tissue
Urea
Antioxidant systems
Extracellular matrices
Hematological parameters
Inflammatory cells
Lipid peroxidation
Oxidative stress markers
S-nitrosoglutathione
Silver
ST - Nanoparticles in treatment of thermal injured rats: Is it safe?
T2 - Journal of Physics: Conference Series
TI - Nanoparticles in treatment of thermal injured rats: Is it safe?
80052049054&doi=10.1088%2f1742-
6596%2f304%2f1%2f012027&partnerID=40&md5=de624b3394e778bf782221d5a40e8e53
VL - 304
ID - 8964
ER -
TY - JOUR
AB - The experimental use of poly (alcohol-vinyl) (PVA) as a skin curative is
increasing widely. However, the use of this hydrogel is challenging due to its
favorable properties for microbiota growth. The association with silver
nanoparticles (AgNPs) as an antimicrobial agent turns the match for PVA as a
dressing, as it focuses on creating a physical barrier to avoid wound dehydration.
When associated with extracellular components, such as the collagen matrix, the
device obtained can create the desired biological conditions to act as a skin
substitute. This study aimed to analyze the anti-microbiological activity and the
in vitro and in vivo responses of a bilaminar device of PVA containing AgNPs
associated with a membrane of collagen-hyaluronic acid (col-HA). Additionally,
mesenchymal stem cells were cultured in the device to evaluate in vitro responses
and in vivo immunomodulatory and healing behavior. The device morphology revealed a
porous pattern that favored water retention and in vitro cell adhesion. Controlled
wounds in the dorsal back of rat skins revealed a striking skin remodeling with new
epidermis fulfilling all previously injured areas after 14 and 28 days. No
infections or significant inflammations were observed, despite increased
angiogenesis, and no fibrosis-markers were identified as compared to controls.
Although few antibacterial activities were obtained, the addition of AgNPs
prevented fungal growth. All results demonstrated that the combination of the
components used here as a dermal device, chosen according to previous miscellany
studies of low/mid-cost biomaterials, can promote skin protection avoiding
infections and dehydration, minimize the typical wound inflammatory responses, and
favor the cellular healing responses, features that give rise to further clinical
trials of the device here developed
AN - rayyan-553780957
AU - Mendes, D.
AU - Hausen, M. A.
AU - Asami, J.
AU - Higa, A. M.
AU - Leite, F. L.
AU - Mambrini, G. P.
AU - Rossi, A. L.
AU - Komatsu, D.
AU - Duek, E. A. D.
IS - 6
KW - Polyvinyls
Alcoholics
Collagen
PY - 2021
SN - 2079-6382
ST - A New Dermal Substitute Containing Polyvinyl Alcohol with Silver
Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches
T2 - ANTIBIOTICS-BASEL
TI - A New Dermal Substitute Containing Polyvinyl Alcohol with Silver
Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches
VL - 10
Y2 - 6
ID - 8965
ER -
TY - JOUR
AB - In the present study, the effects of 10- or 100-nm silica oxide (SiO2) NPs on
human peripheral blood mononuclear cells (PBMC) were examined. Cytotoxic effects
and oxidative stress effects, including glutathione (GSH) depletion, the formation
of protein radical species, and proinflammatory cytokine responses, were measured.
PBMC exposed to 10-nm NP concentrations from 50 to 4,000 ppm showed concentration-
response increases in cell death; whereas, for 100-nm NPs, PBMC viability was not
lost at <500 ppm. Interestingly, 10-nm NPs were more cytotoxic and induced more
oxidative stress than 100-nm NPs. Immunoelectron micrographs show the cellular
distribution of GSH and NPs. As expected based on the viability data, the 10-nm NPs
disturbed cell morphology to a greater extent than did the 100-nm NPs. Antibody to
the radical scavenger, 5, 5-dimethyl-1-pyrroline N-oxide (DMPO), was used for
Western blot analysis of proteins with radicals; more DMPO proteins were found
after exposure to 10-nmNPs than 100-nm NPs. Examination of cytokines (TNF-alpha,
IL-1ra, IL-6, IL-8, IL-1 beta, and IFN-gamma) indicated that different ratios of
cytokines were expressed and released after exposure to 10- and 100-nm NPs. IL-1
beta production was enhanced by 10- and 100-nm NPs;, the cytotoxicity of the NPs
was associated with an increase in the IL-1 beta/IL-6 ratio and 100-nm NPs at
concentrations that did not induce loss of cell viability enhanced IL-1 beta and
IL-6 to an extent similar to phytohemagglutinin (PHA), a T cell mitogen. In
conclusion, our results indicate that SiO2 NPs trigger a cytokine inflammatory
response and induce oxidative stress in vitro, and NPs of the same chemistry, but
of different sizes, demonstrate differences in their intracellular distribution and
immunomodulatory properties, especially with regard to IL-1 beta and IL-6
expression.
AN - rayyan-553780958
AU - Mendoza, A.
AU - Torres-Hernandez, J. A.
AU - Ault, J. G.
AU - Pedersen-Lane, J. H.
AU - Gao, D. H.
AU - Lawrence, D. A.
DO - 10.1007/s12192-014-0502-y
IS - 6
KW - Humanities
Humanism
Humans
Oxidative Stress
Inflammation
PY - 2014
SN - 1355-8145 1466-1268
SP - 777-790
ST - Silica nanoparticles induce oxidative stress and inflammation of human
peripheral blood mononuclear cells
T2 - CELL STRESS & CHAPERONES
TI - Silica nanoparticles induce oxidative stress and inflammation of human
peripheral blood mononuclear cells
VL - 19
Y2 - 11
ID - 8966
ER -
TY - JOUR
AB - The ability of pathogenic bacteria to develop resistance mechanisms to avoid
the antimicrobial potential of antibiotics has become an increasing problem for the
healthcare system. The search for more effective and selective antimicrobial
materials, though not harmful to mammalian cells, seems imperative. Herein we
propose the use of gold-chitosan nanocomposites as effective bactericidal materials
avoiding damage to human cells. Nanocomposites were obtained by taking advantage of
the reductive and stabilizing action of chitosan solutions on two different gold
precursor concentrations. The resulting nanocomposites were added at different
final concentrations to a coculture model formed by Gram-positive (Staphylococcus
aureus) or Gram-negative (Escherichia coli) bacteria and human macrophages. Gold
chitosan colloids exhibited superior bactericidal ability against both bacterial
models without showing cytotoxicity on human cells at the concentrations tested.
Morphological and in vitro viability studies supported the feasibility of the
infection model here described to test novel bactericidal nanomaterials. Flow
cytometry and scanning electron microscopy analyses pointed to the disruption of
the bacterial wall as the lethal mechanism. Data obtained in the present study
suggest that gold chitosan nanocomposites are powerful and promising nanomaterials
for reducing bacteria associated infections, respecting the integrity of mammalian
cells, and displaying high selectivity against the studied bacteria.
AN - rayyan-553780959
AU - Mendoza, G.
AU - Regiel-Futyra, A.
AU - Andreu, V.
AU - Sebastian, V.
AU - Kyziol, A.
AU - Stochel, G.
AU - Arruebo, M.
DO - 10.1021/acsami.6b15123
IS - 21
KW - Humanities
Humanism
Humans
Macrophages
Bacteria
PY - 2017
SN - 1944-8244 1944-8252
SP - 17693-17701
ST - Bactericidal Effect of Gold-Chitosan Nanocomposites in Coculture Models of
Pathogenic Bacteria and Human Macrophages
TI - Bactericidal Effect of Gold-Chitosan Nanocomposites in Coculture Models of
Pathogenic Bacteria and Human Macrophages
VL - 9
Y2 - 5 y3 - 31
ID - 8967
ER -
TY - JOUR
AB - Nanotechnology is emerging as a promising modality for cancer treatment;
however, in the realm of cancer prevention, its full utility has yet to be
determined. Here, we discuss the potential of integrating nanotechnology in cancer
prevention to augment early diagnosis, precision targeting, and controlled release
of chemopreventive agents, reduced toxicity, risk/response assessment, and
personalized point-of-care monitoring. Cancer is a multistep, progressive disease;
the functional and acquired characteristics of the early precancer phenotype are
intrinsically different from those of a more advanced anaplastic or invasive
malignancy. Therefore, applying nanotechnology to precancers is likely to be far
more challenging than applying it to established disease. Frank cancers are more
readily identifiable through imaging and biomarker and histopathologic assessment
than their precancerous precursors. In addition, prevention subjects routinely have
more rigorous intervention criteria than therapy subjects. Any nanopreventive agent
developed to prevent sporadic cancers found in the general population must exhibit
a very low risk of serious side effects. In contrast, a greater risk of side
effects might be more acceptable in subjects at high risk for cancer. Using
nanotechnology to prevent cancer is an aspirational goal, but clearly identifying
the intermediate objectives and potential barriers is an essential first step in
this exciting journey. ©2014 American Association for Cancer Research.
AN - rayyan-553780960
AU - Menter, D. G.
AU - Patterson, S. L.
AU - Logsdon, C. D.
AU - Kopetz, S.
AU - Sood, A. K.
AU - Hawk, E. T.
DO - 10.1158/1940-6207.CAPR-14-0079
IS - 10
KW - Anti-Inflammatory Agents
Anticarcinogenic Agents
Chemoprevention
Humans
Immune System
Liposomes
Nanomedicine
Nanotechnology
Neoplasms
Phenotype
Risk Assessment
RNA Interference
antiinfective agent
asparaginase macrogol
biological marker
celecoxib
chitosan
cisplatin
cytarabine
daunorubicin
doxorubicin
drug carrier
fluorouracil
gold nanoparticle
ingenol mebutate
liposome
nanotube
nucleotide
paclitaxel
polymer
prostate specific membrane antigen
quantum dot
raloxifene
silicon dioxide
silver nanoparticle
small interfering RNA
tamoxifen
unindexed drug
Wart virus vaccine
biomaterial
brain tumor
breast metastasis
cancer prevention
cancer risk
cancer therapy
chemoprophylaxis
diagnostic accuracy
drug response
early diagnosis
histopathology
human
immunomodulation
nanoencapsulation
nanomedicine
nanotechnology
non small cell lung cancer
nonhuman
particle size
phenotype
precancer
Review
risk assessment
surface charge
chemistry
immune system
procedures
RNA interference
PY - 2014
SP - 973-992
ST - Convergence of nanotechnology and cancer prevention: Are we there yet?
T2 - Cancer Prevention Research
TI - Convergence of nanotechnology and cancer prevention: Are we there yet?
84907526151&doi=10.1158%2f1940-6207.CAPR-14-
0079&partnerID=40&md5=295985f10c4409ea84cf94503f6f0348
VL - 7
ID - 8968
ER -
TY - JOUR
AB - Psoriasis is a chronic Th1/Th17 lymphocytes-mediated inflammatory skin
disease, in which epidermal keratinocytes exhibit a peculiar senescent state,
resistance to apoptosis and the acquisition of senescence-associated secretory
phenotype (SASP). SASP consists of the release of soluble factors, including
IGFBPs, that exert extracellular and intracellular functions in IGF-dependent or
independent manner. In this report, we investigated the expression and function of
IGFBP2 in senescent keratinocytes isolated from the skin of patients with plaque
psoriasis. We found that IGFBP2 is aberrantly expressed and released by these cells
in vivo, as well as in vitro in keratinocyte cultures undergoing progressive
senescence, and it associates with the cyclin-dependent kinase inhibitors p21 and
p16 expression. For the first time, we provide evidence for a dual action of IGFBP2
in psoriatic keratinocytes during growth and senescence processes. While
extracellular IGFBP2 counter-regulates IGF-induced keratinocyte hyper-
proliferation, intracellular IGFBP2 inhibits apoptosis by interacting with p21 and
protecting it from ubiquitin-dependent degradation. Indeed, we found that
cytoplasmic p21 sustains anti-apoptotic processes, by inhibiting pro-caspase 3
cleavage and JNK phosphorylation in senescent psoriatic keratinocytes. As a
consequence, abrogation of p21, as well as that of IGFBP2, found to stabilize
cytoplasmic p21 levels, lead to the restoration of apoptosis mechanisms in
psoriatic keratinocytes, commonly observed in healthy cells.
AN - rayyan-553780961
AU - Mercurio, L.
AU - Lulli, D.
AU - Mascia, F.
AU - Dellambra, E.
AU - Scarponi, C.
AU - Morelli, M.
AU - Valente, C.
AU - Carbone, M. L.
AU - Pallotta, S.
AU - Girolomoni, G.
AU - Albanesi, C.
AU - Pastore, S.
AU - Madonna, S.
DO - 10.18632/aging.103045
IS - 8
KW - Insulin-Like Growth Factor I
Somatomedins
Proto-Oncogene Proteins p21(ras)
PY - 2020
SN - 1945-4589
SP - 6823-6851
ST - Intracellular Insulin-like growth factor binding protein 2 (IGFBP2)
contributes to the senescence of keratinocytes in psoriasis by stabilizing
cytoplasmic p21
T2 - AGING-US
TI - Intracellular Insulin-like growth factor binding protein 2 (IGFBP2)
contributes to the senescence of keratinocytes in psoriasis by stabilizing
cytoplasmic p21
VL - 12
Y2 - 4 y3 - 30
ID - 8969
ER -
TY - JOUR
AB - Apoptosis is a permanent and dynamic physiological process by which an
organism eliminates the undesirable cells without causing an inflammatory response.
The objective of this work was to study the expression of FAS, DR4 and other
members of the TNF-R1 superfamily extrinsic route apoptotic receptors the DNA
fragmentation and the cellular apoptosis in placental samples at the early, mid and
late pregnancy on +/- 30, +/- 55 and +/- 114 gestational days, respectively. We
used placental histological sections of samples fixed in buffered saline
formaldehyde. Immunohistochemical techniques were performed to detect the apoptotic
receptors, whereas the DNA fragmentation was detected by TUNEL reaction and
apoptotic cellular ultrastructure was detected by TEM conventional techniques.
Apoptosis related receptors were immunolocalized in the early pig gestation and
correlated with apoptosis, suggesting a role in the cellular remodelling of the
placenta. At gestation day 55, apoptosis might be correlated to FAS route, but not
by DR4-mediating pathway. At the end of gestation, increased apoptosis and both
receptors markers were detected showing cellular death due to the extrinsic route
through FAS and DR4 receptors. In conclusion, the immunolocalization of FAS and TNF
R-1 receptors along the pig placental development correlates with TUNEL reaction
and with apoptotic ultrastructure observed by TEM and seems to occur through
different pathways along gestation. La apoptosis es un proceso fisiológico,
dinámico y permanente a través del cual un organismo elimina células indeseables
sin provocar una respuesta inflamatoria. El objetivo del presente trabajo fue
estudiar la expresión de los receptores de la vía extrínseca de apoptosis, FAS, DR4
y otros miembros de la superfamilia TNF-R1, la fragmentación del ADN y la apoptosis
celular a través de TEM, en muestras placentarias del inicio, la mitad y el final
de la gestación, hacia el día +/- 30, +/- 55 y +/- 114 de preñez, respectivamente.
Se realizaron cortes histológicos de las muestras placentarias fijadas en formol
tamponado. Para la detección de los receptores de apoptosis se realizaron técnicas
inmunohistoquímicas, para el estudio de la fragmentación del ADN se utilizó el
ensayo TUNEL y para el análisis de la ultraestructura celular apoptótica la técnica
convencional de TEM. La inmunolocalización de los receptores de muerte celular al
inicio de la preñez porcina sugiere el rol de la apoptosis en la remodelación
celular placentaria. Hacia el día 55 de preñez, la apoptosis detectada ocurriría
únicamente a través de la vía del receptor FAS, no del receptor DR4. Al final de la
gestación, se detectó un incremento de la apoptosis y la expresión de ambos
receptores, indicando que la muerte celular a través de la vía de señalización
extrínseca estaría inducida por los receptores FAS y DR4. En conclusión, la
inmunolocalización de los receptores FAS y otros miembros del TNF-R1, los
resultados de TUNEL y la ultraestructura celular apoptótica observada en la
placentación porcina, indican que la apoptosis detectada ocurre por diferentes vías
de inducción a lo largo de la gestación.
AN - rayyan-553780962
AU - Merkis, C.
AU - Cristofolini, A.
AU - Sanchis, E.
AU - Koncurat, M.
DO - 10.1590/S0717-95022010000300026
IS - 3
KW - Apoptosis receptors
DR4 and TNF-R1 family
DR4 y superfamilia TNF-R1
FAS/CD95
Pig placenta
Placenta porcina
Receptors de apoptosis
Antigens, CD95
LA - en
PY - 2010
SN - 0717-9367
SP - 829-834
ST - Expression of death cellular receptors FAS/CD95 and DR4 during porcine
placentation
TI - Expression of death cellular receptors FAS/CD95 and DR4 during porcine
placentation
95022010000300026
VL - 28
Y2 - 9 y3 - 1
ID - 8970
ER -
TY - COMP
AB - An in vitro method for the cytotoxicity testing of endodontic materials is
described which aims to simulate the clinical situation. Materials can be tested in
the presence or absence of a compacted layer of dentine chips mimicking the
periapical dentine plug. A total of twelve materials were tested. In the absence of
dentine, Kloroperka, Biocalex, Diaket and Endomethasone were slightly cytotoxic;
AH26 with and without silver, Sealapex, Tubliseal and Kerr's pulp canal sealer were
moderately cytotoxic, while Forfenan, Spad and Kri paste were strongly cytotoxic.
In the presence of dentine the cytotoxicity of these materials was considerably
reduced, with the exception of Endomethasone, Forfenan, Spad and Kri paste. The
method provides a satisfactory alternative to implantation testing and is an
inexpensive and reproducible test system in which dentine can be incorporated.
AN - rayyan-553782213
AU - Meryon, S. D.
AU - Brook, A. M.
CY - England
DO - 10.1111/j.1365-2591.1990.tb00101.x
ET - 4
J2 - Int Endod J
Balsams
Bismuth
Calcium Hydroxide
Cells, Cultured
Dental Cements/*toxicity
Dentin/drug effects
Dexamethasone
Drug Combinations
*Epoxy Resins
Formaldehyde
Gutta-Percha
*Hydrocortisone
Methacrylates
Methenamine
Polymers
Polyvinyls
Resorcinols
*Salicylates
Silver
Thymol/analogs & derivatives
Titanium
Zinc Oxide
*Zinc Oxide-Eugenol Cement
LA - eng
N1 - Dental School, University of Birmingham, UK.
PY - 1990
SN - 0143-2885 (Print)
SP - 203-10
ST - In vitro comparison of the cytotoxicity of twelve endodontic materials using
a new technique
T2 - International endodontic journal
TI - In vitro comparison of the cytotoxicity of twelve endodontic materials using
a new technique
VL - 23
Y2 - 7
ID - 10123
ER -
TY - JOUR
AB - Background: Nanosilver particles of which antibacterial and antifungal
properties have been shown in various in vitro and in vivo studies are used in many
medical and dental fields for the prevention of infection. In this study, it is
intended to evaluate the biocompatibility of nanosilver-coated brackets. Methods:
Nanosilver coating process was applied to the standard orthodontic brackets by a
physical vapor deposition system. Brackets were coated with nanosilver particles of
1 mu thickness. A total of 12 Wistar Albino rats were included in the study (six)
and control (six) groups. For the study and control groups, four nanosilver-coated
and four standard brackets were aseptically implanted subcutaneously in the dorsal
region of each rat. The brackets were removed with the surrounding tissues on days
7, 14, 30, and 60. The specimens were evaluated for inflammatory response. Results:
No significant difference was found in terms of tissue reaction between the study
and control groups. On day 7, randomly distributed brown-black granules were seen
in the granulation tissue adjacent to the bracket in the study group. These foreign
particles continued along the bracket cavity in a few samples, but the inflammatory
response was insignificant between the groups. Mast cell count was found to be
significantly smaller only on day 7 in the study group than in the control group.
Conclusions: Nanosilver-coated orthodontic brackets were found to be similar with
the standard type concerning inflammation. Further researches are needed with
regard to the assessment of the brown-black granules, especially on the deposition
of the vessel walls.
AN - rayyan-553780964
AU - Metin-Gursoy, G.
AU - Taner, L.
AU - Baris, E.
DO - 10.1186/s40510-016-0152-y
PY - 2016
SN - 2196-1042
ST - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
T2 - PROGRESS IN ORTHODONTICS
TI - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
VL - 17
Y2 - 12 y3 - 5
ID - 8972
ER -
TY - JOUR
AB - BACKGROUND: Nanosilver particles of which antibacterial and antifungal
properties have been shown in various in vitro and in vivo studies are used in many
medical and dental fields for the prevention of infection. In this study, it is
intended to evaluate the biocompatibility of nanosilver-coated brackets. METHODS:
Nanosilver coating process was applied to the standard orthodontic brackets by a
physical vapor deposition system. Brackets were coated with nanosilver particles of
1 μ thickness. A total of 12 Wistar Albino rats were included in the study (six)
and control (six) groups. For the study and control groups, four nanosilver-coated
and four standard brackets were aseptically implanted subcutaneously in the dorsal
region of each rat. The brackets were removed with the surrounding tissues on days
7, 14, 30, and 60. The specimens were evaluated for inflammatory response. RESULTS:
No significant difference was found in terms of tissue reaction between the study
and control groups. On day 7, randomly distributed brown-black granules were seen
in the granulation tissue adjacent to the bracket in the study group. These foreign
particles continued along the bracket cavity in a few samples, but the inflammatory
response was insignificant between the groups. Mast cell count was found to be
significantly smaller only on day 7 in the study group than in the control group.
CONCLUSIONS: Nanosilver-coated orthodontic brackets were found to be similar with
the standard type concerning inflammation. Further researches are needed with
regard to the assessment of the brown-black granules, especially on the deposition
of the vessel walls.
AN - rayyan-553782129
AU - Metin-Gürsoy, G.
AU - Taner, L.
AU - Barış, E.
DO - 10.1186/s40510-016-0152-y
IS - 1
J2 - Prog Orthod
KW - Animals
Coated Materials, Biocompatible/*chemistry/*pharmacology
Dental Alloys/chemistry
Dental Implants
Epithelium/drug effects/pathology
Female
Inflammation
Lymphocytes/drug effects
Mandible
Mast Cells/drug effects/pathology
*Materials Testing
Metal Nanoparticles/chemistry
Models, Animal
Orthodontic Appliance Design/instrumentation
*Orthodontic Brackets
Plasma Cells/drug effects/pathology
Rats
Rats, Wistar
Silver Compounds/chemistry/pharmacology
Surface Properties
Time Factors
LA - eng
N1 - Department of Orthodontics, Faculty of Dentistry, Gazi University, Bişkek
cad. 1. Sok. No: 4 06510, Emek, Ankara, Turkey. gamgursoy@gmail.com.; Department of
Orthodontics, Faculty of Dentistry, Gazi University, Bişkek cad. 1. Sok. No: 4
06510, Emek, Ankara, Turkey.; Department of Oral Pathology, Faculty of Dentistry,
Gazi University, Bişkek cad. 1. Sok. No: 4 06510, Emek, Ankara, Turkey.
PY - 2016
SP - 39
ST - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
T2 - Progress in orthodontics
TI - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
VL - 17
Y2 - 12
ID - 10040
ER -
TY - JOUR
AB - Ginsenoside compound K (CK) has been shown to exhibit anti-inflammatory
properties. In this study, to encourage biomedical applications of biosynthesized
gold nanoparticles (AuNPs) with anti-inflammatory effects, AuNPs loaded with
ginsenoside compound K were prepared using a self-assembly technique with chitosan
as the carrier. Optimal conditions for chitosan-ginsenoside CK-gold nanoparticles
(CS-CK-AuNPs) formation were monitored using UV-Vis absorption spectroscopy. The
physicochemical characterization of CS-CK-AuNPs was performed using FE-TEM, FE-SEM,
XRD, DLS, FTIR and NMR techniques. In the stability test, CS-CK-AuNPs did not show
any significant changes up to 4 weeks. Fluorescence imaging demonstrated that CS-
CK-AuNPs promoted cellular uptake in vitro, but did not exhibit significant
cytotoxicity at concentrations below 40 mu g/mL. Additionally, the CS-CK-AuNPs
inhibited NO production, and reduced the expression and secretion of inflammatory
cytokines (IL-1 beta, IL-6, and TNF-alpha) via inhibition of the nuclear factor-
kappaB (NF-kappa B) pathway in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
Thus, CS-CK-AuNPs are novel candidates for developing antiinflammatory agent. This
study also confirms the superiority of chitosan AuNPs as oral delivery vehicles for
inflammation-related diseases.
AN - rayyan-553780965
AU - Mi, X. J.
AU - Choi, H. S.
AU - Park, H. R.
AU - Kim, Y. J.
DO - 10.1016/j.ijbiomac.2022.05.177
PY - 2022
SN - 0141-8130 1879-0003
SP - 247-258
ST - Structural characterization and anti-inflammatory properties of green
synthesized chitosan/compound K-gold nanoparticles
T2 - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
TI - Structural characterization and anti-inflammatory properties of green
synthesized chitosan/compound K-gold nanoparticles
VL - 213
Y2 - 7 y3 - 31
ID - 8973
ER -
TY - JOUR
AB - For decades, nanomedicines have been reported as a potential means to
overcome the limitations of conventional drug delivery systems by reducing side
effects, toxicity and the non-ideal pharmacokinetic behaviour typically exhibited
by small molecule drugs. However, upon administration many nanoparticles prompt
induction of host inflammatory responses due to recognition and uptake by
macrophages, eliminating up to 95% of the administered dose. While significant
advances in nanoparticle engineering and consequent therapeutic efficacy have been
made, it is becoming clear that nanoparticle recognition by the mononuclear
phagocyte system (MPS) poses an impassable junction in the current framework of
nanoparticle development. Hence, this has negative consequences on the clinical
translation of nanotechnology with respect to therapeutic efficacy, systemic
toxicity and economic benefit. In order to improve the translation of nanomedicines
from bench-to-bedside, there is a requirement to either modify nanomedicines in
terms of how they interact with intrinsic processes in the body, or modulate the
body to be more accommodating for nanomedicine treatments. Here we provide an
overview of the current standard for design elements of nanoparticles, as well as
factors to consider when producing nanomedicines that have minimal MPS-nanoparticle
interactions; we explore this landscape across the cellular to tissue and organ
levels. Further, rather than designing materials to suit the body, a growing
research niche involves modulating biological responses to administered
nanomaterials. We here discuss how developing strategic methods of MPS 'pre-
conditioning' with small molecule or biological drugs, as well as implementing
strategic dosing regimens, such as 'decoy' nanoparticles, is essential to
increasing nanoparticle therapeutic efficacy. By adopting such a perspective, we
hope to highlight the increasing trends in research dedicated to improving
nanomedicine translation, and subsequently making a positive clinical impact.
AN - rayyan-553780969
AU - Mills, J. A.
AU - Liu, F. F.
AU - Jarrett, T. R.
AU - Fletcher, N. L.
AU - Thurecht, K. J.
DO - 10.1039/d2bm00181k
IS - 12
KW - Phagocytes
Nanoparticles
PY - 2022
SN - 2047-4830 2047-4849
SP - 3029-3053
ST - Nanoparticle based medicines: approaches for evading and manipulating the
mononuclear phagocyte system and potential for clinical translation
T2 - BIOMATERIALS SCIENCE
TI - Nanoparticle based medicines: approaches for evading and manipulating the
mononuclear phagocyte system and potential for clinical translation
VL - 10
Y2 - 6 y3 - 14
ID - 8977
ER -
TY - JOUR
AB - Emerging nanoscience allows us to take advantage of the improved evolutionary
components and apply today's advanced characterization and fabrication techniques
to solve environmental and biological problems. Despite the promise that
nanotechnology will improve our lives, the potential risks of technology remain
largely uncertain. The lack of information on bio-impacts and the absence of
consistent standards are the limitations of using metal-based nanoparticles (mNPs)
for existing applications. To analyze the role played by the mNPs physicochemical
characteristics and tactics to protect live beings, the field of nanotoxicology
nowadays is focused on collecting and analyzing data from in vitro and in vivo
investigations. The degree of reactive oxygen species (ROS) and oxidative stress
caused by material nanoparticles (NPs) depends on many factors, such as size,
shape, chemical composition, etc. These characteristics enable NPs to enter cells
and interact with biological macromolecules and cell organelles, resulting in
oxidative damage, an inflammatory response, the development of mitochondrial
dysfunction, damage to genetic material, or cytotoxic effects. This report explored
the mechanisms and cellular signaling cascades of mNPs-induced oxidative stress and
the relevant health consequences.
AN - rayyan-553780973
AU - Min, Y. H.
AU - Suminda, G. G. D.
AU - Heo, Y.
AU - Kim, M.
AU - Ghosh, M.
AU - Son, Y. O.
DO - 10.3390/antiox12030703
IS - 3
PY - 2023
SN - 2076-3921
ST - Metal-Based Nanoparticles and Their Relevant Consequences on Cytotoxicity
Cascade and Induced Oxidative Stress
T2 - ANTIOXIDANTS
TI - Metal-Based Nanoparticles and Their Relevant Consequences on Cytotoxicity
Cascade and Induced Oxidative Stress
VL - 12
Y2 - 3
ID - 8981
ER -
TY - CHAP
AB - Nanotechnology has emerged as a key player in various disciplines of science
and technology. Some of the most exciting applications are in the field of
biomedicine—for theranostics (for combined diagnostic and therapeutic purposes) and
for exploration of biological systems. A detailed understanding of the molecular
interactions between nanoparticles and biological nanomachinery—macromolecules,
membranes, and intracellular organelles—is crucial for obtaining adequate
information on mechanisms of action of nanomaterials and a perspective on the long-
term effects of these materials and their possible toxicological outcomes. This
chapter focuses on the toxicological aspects of nanoparticles and carbon nanotubes
on animals, humans, plants, and aquatic life. Generally, the harmful effects of
nanoparticles arise from the combination of various factors, two of which are
particularly important: (a) the high surface area and (b) the intrinsic toxicity of
the surface. In contrast with conventional particles of larger mean diameter,
nanoparticles under 100nm can potentially be more toxic to the lung (portal of
entry), can redistribute from their site of deposition, can escape from the normal
phagocytic defenses, and can modify the structure of proteins. Therefore,
nanoparticles can activate inflammatory and immunologic responses and may affect
the normal tissue function. CNT, in the context of toxicology, can be classified as
“nanoparticles” due to their nanoscale dimensions; therefore, unexpected
toxicological effects upon contact with biological systems may be induced. © 2019
AN - rayyan-553780974
AU - Mishra, R.
AU - Militky, J.
AU - Arumugam, V.
DO - 10.1016/B978-0-08-102609-0.00008-0
KW - Antibacterial nanoparticles
Carbon nanotubes
Inhalation
Life-cycle assessment
MWCNT
Nanoparticles
Toxicology
PY - 2018
SP - 355-385
ST - Nanorisks and nanohazards
T2 - Nanotechnology in Textiles: Theory and Application
TI - Nanorisks and nanohazards
85142090051&doi=10.1016%2fB978-0-08-102609-0.00008-
0&partnerID=40&md5=4a45e0051aecb7a8cf6f5e6d5fcf3c15
ID - 8982
ER -
TY - JOUR
AB - Copper oxide nanoparticles with different shapes were used to examine the
effect of shape on the various physicochemical properties (reactivity, aggregation,
suspension stability) and to examine the behaviour by which CuO nanoparticles
exhibit their biological response towards alveolar type-I cells. The different
shapes examined in this study include spherical-, rod- and spindle-shaped platelet
particles. In vitro dissolution studies (7 days) in 1 mM NaNO3 matrix showed a
marked difference in dissolved Cu release between the nanoparticles. However, in
serum-free cell-culture media (exposure media to cells), the particles' dissolution
was found to be significantly enhanced with close to complete dissolution reported
for all particle types. Biological studies showed both shape and size of the CuO
nanoparticles tested to have a significant effect on TT-1 cell viability and
release of pro-inflammatory cytokines IL-6 and IL-8. This study shows a complex
interplay between particulate and dissolved species triggering the biological
response. Upon immediate exposure of CuO nanoparticles of different shapes, the
particulate form contributes towards the toxicity. However, for any biological
response observed over and beyond a period of 24 h, the dissolved fraction becomes
significant.
AN - rayyan-553780977
AU - Misra, S. K.
AU - Nuseibeh, S.
AU - Dybowska, A.
AU - Berhanu, D.
AU - Tetley, T. D.
AU - Valsami-Jones, E.
DO - 10.3109/17435390.2013.796017
IS - 4
PY - 2014
SN - 1743-5390 1743-5404
SP - 422-432
ST - Comparative study using spheres, rods and spindle-shaped nanoplatelets on
dispersion stability, dissolution and toxicity of CuO nanomaterials
T2 - NANOTOXICOLOGY
TI - Comparative study using spheres, rods and spindle-shaped nanoplatelets on
dispersion stability, dissolution and toxicity of CuO nanomaterials
VL - 8
Y2 - 6
ID - 8985
ER -
TY - JOUR
AB - Nanoparticles(NPs) are of particle sizes lesser than 100nm and are insoluble
the field which deal with the handling of these particles is coined as
"Nanotechnology" as their particle size is smaller, they can penetrate easily
therefore they are applied in various medical fields, drug delivery and in
dentistry as they have antimicrobial property, reduces friction, anti-inflammatory
and antioxidant property. Many studies have been done to evaluate its application
and its cytotoxicity by varying its concentration and various studies have been
done to evaluate its physical property. Therefore, it is of interest to describe
concepts of nanoparticles, mode of action, tissue reaction and its application in
orthodontics.
AN - rayyan-553780978
AU - Missier, M. S.
AU - Ramakrishnan, M.
AU - Paulpandian, S. D. S.
AU - Pringle, J.
DO - 10.6026/97320630019014
IS - 1
PY - 2023
SN - 0973-8894 0973-2063
SP - 14-18
ST - Application of nanoparticles in Dentistry
T2 - BIOINFORMATION
TI - Application of nanoparticles in Dentistry
VL - 19
Y2 - 1
ID - 8986
ER -
TY - CHAP
AB - The health effects of silver nanoparticles (AgNPs) have not been well
investigated, despite AgNPs now being widely used in consumer products. We
introduce living environment, analysis, metabolic behavior, toxicity, and human
health effect of AgNPs in comparison to silver nitrate (AgNO3). The American
Conference of Governmental Industrial Hygienists (ACGIH) has established separate
threshold limit values (TLV) for metallic silver (0.1 mg/m3) and soluble compounds
of silver (0.01 mg/m3). Argyria and argyrosis are chronic disorders of skin
microvessels and eyes in humans, and these disorders reportedly develop following
extended oral and inhalational exposure to Ag. In mammals, AgNO3 and AgNPs
increased the number of the total cells, neutrophils, and pro-inflammatory cytokine
production "IL-1β," and these were distributed in the lung, kidney, and liver. The
amount of Ag in the metallothionein (MT)-bound form was related in cellular
behavior and toxicity of AgNPs and AgNO3. The cytotoxic effect of AgNPs is a simple
function of neither the number nor total surface area. Although the effect may vary
among the cell types and the culture conditions, AgNPs were transported to
lysosomes and only gradually dissolved in mammals, causing milder inflammatory
stimulation. © Springer Japan 2016. All rights are reserved.
AN - rayyan-553780979
AU - Miyayama, T.
AU - Arai, Y.
AU - Hirano, S.
DO - 10.1007/978-4-431-55732-6_7
KW - Lysosome
Macrophages
Metallothionein
Silver ion
Silver nanoparticle
PY - 2015
SP - 137-147
ST - Health effects of silver nanoparticles and silver ions
T2 - Biological Effects of Fibrous and Particulate Substances
TI - Health effects of silver nanoparticles and silver ions
84956475031&doi=10.1007%2f978-4-431-55732-
6_7&partnerID=40&md5=5b4738c2cb67b83dd127ca292445b0ff
ID - 8987
ER -
TY - JOUR
AB - This study consisted of developing a dressing loaded with silver (Ag) and
ibuprofen (IBU) that provides a dual therapy, antibacterial and antalgic, intended
for infected painful wounds. There-fore, non-woven polyethyleneterephtalate (PET)
textiles nonwovens were pre-treated by cyclodextrin crosslinked with citric acid by
a pad/dry/cure process. Then, textiles were impregnated in silver so-lution
followed by a thermal treatment and were then coated by Layer-by-Layer (L-b-L)
deposition of a polyelectrolyte multilayer (PEM) system consisting of anionic
water-soluble poly(betacyclodextrin citrate) (PCD) and cationic chitosan. Finally,
ibuprofen lysinate (IBU-L) was loaded on the PEM coating. We demonstrated the
complexation of IBU with native βCD and PCD by phase solubility diagram and1 H NMR.
PEM system allowed complete IBU-L release in 6 h in PBS pH 7.4 batch (USP IV). On
the other hand, microbiological tests demonstrated that loaded silver induced
bacterial reduction of 4 Log10 against S. aureus and E. coli and tests revealed
that ibuprofen lysinate loading did not interfere with the antibacterial properties
of the dressing. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553780982
AU - Mogrovejo-Valdivia, A.
AU - Maton, M.
AU - Garcia-Fernandez, M. J.
AU - Tabary, N.
AU - Chai, F.
AU - Neut, C.
AU - Martel, B.
AU - Blanchemain, N.
IS - 7
Antibacterial
Cyclodextrins
Drug delivery system
Layer by layer
Wound dressing
antibiotic agent
antiinfective agent
chitosan
citric acid
cyclodextrin
ibuprofen
ibuprofen lysine
polycaprolactone
polyelectrolyte
polyethylene terephthalate
silver
silver nanoparticle
silver sulfate
unclassified drug
water
Article
biological activity
cell viability
chemical reaction kinetics
colony forming unit
complex formation
controlled study
cross linking
cytotoxicity
diffusion
disk diffusion
drug absorption
drug activity
drug release
drug solubility
echography
electrospinning
encapsulation
Escherichia coli
in vitro study
isotherm
isothermal amplification
layer by layer deposition
mathematical analysis
microbiological examination
nonhuman
pH
solubility
ultraviolet microscopy
wound healing
zone of inhibition
PY - 2021
ST - In vitro microbiological and drug release of silver/ibuprofen loaded wound
dressing designed for the treatment of chronically infected painful wounds
T2 - Antibiotics
TI - In vitro microbiological and drug release of silver/ibuprofen loaded wound
dressing designed for the treatment of chronically infected painful wounds
85110279038&doi=10.3390%2fantibiotics10070805&partnerID=40&md5=9014356407e6e2eb7403
f6c0b262f120
VL - 10
ID - 8990
ER -
TY - JOUR
AB - Engineered nanoparticles (ENP), which could be composed of inorganic metals,
metal oxides, metalloids, organic biodegradable and inorganic biocompatible
polymers, are being used as carriers for vaccine and drug delivery. There is also
increasing interest in their application as delivery agents for the treatment of a
variety of lung diseases. Although many studies have shown ENP can be effectively
and safely used to enhance the delivery of drugs and vaccines in the periphery,
there is concern that some ENP could promote inflammation, with unknown
consequences for lung immune homeostasis. In this study, we review research on the
effects of ENP on lung immunity, focusing on recent studies using diverse animal
models of human lung disease. We summarize how the inflammatory and immune response
to ENP is influenced by the diverse biophysical and chemical characteristics of the
particles including composition, size and mode of delivery. We further discuss
newly described unexpected beneficial properties of ENP administered into the lung,
where biocompatible polystyrene or silver nanoparticles can by themselves decrease
susceptibility to allergic airways inflammation. Increasing our understanding of
the differential effects of diverse types of nanoparticles on pulmonary immune
homeostasis, particularly previously underappreciated beneficial outcomes, supports
rational ENP translation into novel therapeutics for prevention and/or treatment of
inflammatory lung disorders. © 2014 Informa Healthcare USA, Inc. All rights
reserved: reproduction in whole or part not permitted.
AN - rayyan-553780984
AU - Mohamud, R.
AU - Xiang, S. D.
AU - Selomulya, C.
AU - Rolland, J. M.
AU - O'Hehir, R. E.
AU - Hardy, C. L.
AU - Plebanski, M.
DO - 10.3109/03602532.2013.859688
IS - 2
KW - Animal models
Delivery vehicles
Immune response
Inflammation
Lung
Particles
Toxicity
Vaccine
Adaptive Immunity
Animals
Drug Carriers
Homeostasis
Humans
Immunity, Innate
Nanoparticles
Particle Size
Pneumonia
Surface Properties
carbon nanotube
drug vehicle
gold nanoparticle
iron oxide
liposome
nanoparticle
polysaccharide
polystyrene
silicon dioxide
silver nanoparticle
titanium dioxide
zinc oxide
drug carrier
adaptive immunity
biocompatibility
complement activation
dendritic cell
homeostasis
human
immune response
immunity
inflammation
innate immunity
lung function
nonhuman
physical chemistry
pneumonia
review
animal
chemistry
drug effects
immunology
lung
particle size
surface property
PY - 2014
SP - 176-190
ST - The effects of engineered nanoparticles on pulmonary immune homeostasis
T2 - Drug Metabolism Reviews
TI - The effects of engineered nanoparticles on pulmonary immune homeostasis
84899439295&doi=10.3109%2f03602532.2013.859688&partnerID=40&md5=9be799d6f4f04f0c138
cdb9ddf2bcb45
VL - 46
ID - 8992
ER -
TY - JOUR
AB - Prostate cancer is one of the most common malignancies among men worldwide.
The main aim of the present work was to clarify the advantages of a nanoformulation
of ayurvedic herbal plants. Specifically, we assessed the improved anticancer
activity of Leucas aspera nanoparticles compared with methanolic crude extract in
PC3 prostate cancer cells and normal cells. L. aspera is a plant that is used in
ayurveda due to the antirheumatic, antipyretic, anti-inflammatory, antibacterial,
anticancer, and cytotoxic activities. Nanoparticles of L. aspera were prepared from
plant methanolic extracts. Cytotoxic effect was studied in the normal and prostate
cancer cells. Size and morphology of the formulated nanoparticles was assessed
using dynamic light scattering and scanning electron microscopy. In vitro
cytotoxicity of L. aspera nanoparticles for PC3 cells was concentration- and time-
dependent. In vitro hemolysis assay, cellular uptake studies, cell aggregation
studies, and cell migration assay established the anticancerous activity of L.
aspera in prostate cancer.
AN - rayyan-553780985
AU - Mohan, A.
AU - Nair, S. V.
AU - Lakshmanan, V. K.
DO - 10.1007/s12010-016-2291-5
IS - 4
KW - Prostatic Neoplasms
PY - 2017
SN - 0273-2289 1559-0291
SP - 1388-1400
ST - Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration
Retarded in Prostate Cancer Cells
T2 - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
TI - Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration
Retarded in Prostate Cancer Cells
VL - 181
Y2 - 4
ID - 8993
ER -
TY - JOUR
AB - The present study deals with the biosynthesis of palladium nanoparticles
(PdNPs) using Agaricus bisporus and exploring its potential biological
applications. The synthesized PdNPs were characterized by UV-visible, FTIR and XRD
techniques. Microscopic analyses revealed the triangular (SEM and AFM) and
spherical (TEM) morphologies of the nanoparticles with nanosize dimension ranging
from 13 to 18 nm. The surface charge of the PdNPs were identified with the help of
zeta potential and found to be negatively charged (- 24.3 mV). The PdNPs exhibited
good antioxidant effect against DPPH free radicals with maximum radical scavenging
activity of 77% using 50 mu g/ml. FTIR spectra of the final DPPH solution depicted
sharp intense signals at 1018 cm(-1) (polysaccharides) and 3342 cm(-1) (phenolic
acids) evidencing the role of these bio functional groups in neutralizing the free
radicals. Antibacterial assay revealed that PdNPs exhibited enhanced growth
inhibition effect against gram positive bacteria (S. auerus; S. pyrogens; B.
subtilis) than gram negative bacterial pathogens (E. aerogenes; K. pneumoniae; P.
vulgaris). Anti-inflammatory activity performed with RBC cells showing 87% of
activity for biosynthesized PdNPs. MTT assay demonstrated that PdNPs exhibited
excellent cytotoxic effect against PK13 cell lines. Maximum growth inhibition of
79% was observed for the maximum dose (50 mu g/ml) with IC50 value of 26.1 mu g/ml.
AN - rayyan-553780986
AU - Mohana, S.
AU - Sumathi, S.
DO - 10.1007/s10876-019-01652-2
IS - 2
PY - 2020
SN - 1040-7278 1572-8862
SP - 391-400
ST - Multi-Functional Biological Effects of Palladium Nanoparticles Synthesized
Using Agaricus bisporus
T2 - JOURNAL OF CLUSTER SCIENCE
TI - Multi-Functional Biological Effects of Palladium Nanoparticles Synthesized
Using Agaricus bisporus
VL - 31
Y2 - 3
ID - 8994
ER -
TY - JOUR
AB - BACKGROUND Zinc oxide nanoparticles play a vital role in diagnostics,
biomolecular detection, and microelectronics. Several conventional methods are used
for synthesis of zinc oxide nanoparticles. But, toxic chemicals are required as
capping agents to maintain stability, thus leading to toxicity in the environment.
Thus, we need to shift to "green synthesis". Hence, this study was conducted to
assess the cytotoxicity, antiinflammatory, and antioxidant activity of zinc oxide
nanoparticles reinforced with clove and cinnamon. METHODS Cytotoxic effect, anti-
inflammatory activity, antioxidant activity of zinc oxide nanoparticles reinforced
with clove and cinnamon extract were assessed using Brine Shrimp Assay, Bovine
Serum Albumin (BSA) and DPPH Assay respectively at 5 mu L, 10 mu L, 20 mu L, 30 mu
L, 50 mu L. RESULTS As the concentration increased, the cytotoxicity of the
nanoparticles increased. Values for anti-inflammatory property of nanoparticles was
higher than the standard values at all concentrations. Percentage of inhibition was
highest at 40 mu L (91.1%) and 50 mu L (90.5%). The values for antioxidant property
of nanoparticles was found to be higher than the standard values at all
concentrations except at 50 mu L. Percentage of inhibition was highest at 20 mu L
(86.2%). CONCLUSIONS Zinc oxide nanoparticles reinforced with clove and cinnamon
extract have a potential as an anti-cancer, anti-inflammatory and antioxidant agent
and can be used as an alternative to commercially available products.
AN - rayyan-553780987
AU - Mohapatra, S.
AU - Leelavathi, L.
AU - Sri Sakthi, D.
AU - Jayashri, P.
DO - 10.14260/jemds/2020/405
IS - 25
KW - Zinc
Antioxidants
PY - 2020
SN - 2278-4748 2278-4802
SP - 1859-1864
ST - Assessment of Cytotoxicity, Anti-Inflammatory and Antioxidant Activity of
Zinc Oxide Nanoparticles Synthesized Using Clove and Cinnamon Formulation - An In-
Vitro Study
T2 - JOURNAL OF EVOLUTION OF MEDICAL AND DENTAL SCIENCES-JEMDS
TI - Assessment of Cytotoxicity, Anti-Inflammatory and Antioxidant Activity of
Zinc Oxide Nanoparticles Synthesized Using Clove and Cinnamon Formulation - An In-
Vitro Study
VL - 9
Y2 - 6 y3 - 22
ID - 8995
ER -
TY - JOUR
AB - Silver was widely used in medicine to treat bacterial infections in the 19th
and early 20th century, up until the discovery and development of the first modern
antibiotics in the 1940s, which were markedly more effective. Since then, every new
antibiotic introduced to the clinic has led to an associated development of drug
resistance. Today, the threat of extensive bacterial resistance to antibiotics has
reignited interest in alternative strategies to treat infectious diseases, with
silver regaining well-deserved renewed attention. Silver ions are highly disruptive
to bacterial integrity and biochemical function, with comparatively minimal
toxicity to mammalian cells. This review focuses on the antimicrobial properties of
silver and their use in synergistic combination therapy with traditional antibiotic
drugs.
AN - rayyan-553780989
AU - Mohler, J. S.
AU - Sim, W.
AU - Blaskovich, M. A. T.
AU - Cooper, M. A.
AU - Ziora, Z. M.
DO - 10.1016/j.biotechadv.2018.05.004
IS - 5
PY - 2018
SN - 0734-9750 1873-1899
SP - 1391-1411
ST - Silver bullets: A new lustre on an old antimicrobial agent
T2 - BIOTECHNOLOGY ADVANCES
TI - Silver bullets: A new lustre on an old antimicrobial agent
VL - 36
Y2 - 9
ID - 8996
ER -
TY - JOUR
AB - BACKGROUND: Burn is the most devastating condition in emergency medicine
leading to chronic disabilities. This study aimed to compare the effect of
Lithospermum officinale, silver sulfadiazine and alpha ointments on healing of burn
wounds in rat. METHODS: Ninety-five rats were divided into 5 groups. Group 1 just
underwent burn injury, and groups 2-5 received alpha ointment, silver sulfadiazine
(SSD), gel base and L. officinale extract, respectively. A hot plate was used for
induction of a standard 3(rd) degree burn wound. Burn wounds were macroscopically
and microscopically evaluated on days 7(th), 14(th) and 21(st) after burn
induction. RESULTS: A decrease in the number of inflammatory cells was noted when
L. officinale and SSD were applied while the most inflammatory response was seen
after administration of alpha ointment. The number of macrophages alone decreased
after burn injury, while the frequency was the most when L. officinale and alpha
ointment were applied. Re-epithelialization, angiogenesis and formation of
granulation tissue were the best in relation to L. officinale and alpha ointment
while, the worst results belonged to burn injury group and SSD regarding
granulation tissue formation. Considering histological assessment, the best results
were observed for scoring of inflammation, re-epithelialization, angiogenesis,
formation of granulation tissue and number of macrophage when L. officinale and
alpha ointment were used after burn injury. CONCLUSION: It can be concluded that
topical application of L. officinale as a non-toxic, inexpensive and easy to
produce herbal can lead to a rapid epithelialization and wound healing and these
findings can be added to the literature on burn wound healing.
AN - rayyan-553782338
AU - Mohtasham Amiri, Z.
AU - Tanideh, N.
AU - Seddighi, A.
AU - Mokhtari, M.
AU - Amini, M.
AU - Shakouri Partovi, A.
AU - Manafi, A.
AU - Hashemi, S. S.
AU - Mehrabani, D.
IS - 3
J2 - World J Plast Surg
KW - Rats
LA - eng
N1 - Guilan Road Trauma Research Center, Department of Community Medicine, School
of Medicine, Guilan University of Medical Sciences, Rasht, Iran.; Stem Cell and
Transgenic Technology Research Center, Shiraz University of Medical Sciences,
Shiraz, Iran.; Department of Pharmacology, School of Medicine, Shiraz University of
Medical Sciences, Shiraz, Iran.; Department of Genetics, Tehran Branch, Islamic
Azad University, Tehran, Iran.; Department of Pathology, School of Medicine, Shiraz
University of Medical Sciences, Shiraz, Iran.; Minimal Invasive Laparascopy
Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.; Guilan Road
Trauma Research Center, Department of Community Medicine, School of Medicine,
Guilan University of Medical Sciences, Rasht, Iran.; Iran University of Medical
Sciences, Tehran, Iran.; Burn and Wound Healing Research Center, Shiraz University
of Medical Sciences, Shiraz, Iran.; Stem Cell and Transgenic Technology Research
Center, Shiraz University of Medical Sciences, Shiraz, Iran.
PY - 2017
SN - 2228-7914 (Print)
SP - 313-318
ST - The Effect of Lithospermum officinale, Silver Sulfadiazine and Alpha
Ointments in Healing of Burn Wound Injuries in Rat
T2 - World journal of plastic surgery
TI - The Effect of Lithospermum officinale, Silver Sulfadiazine and Alpha
Ointments in Healing of Burn Wound Injuries in Rat
VL - 6
Y2 - 9
ID - 10246
ER -
TY - CONF
AN - rayyan-553780991
AU - Moldwin, R. M.
AU - Sant, G. R.
DA - 2002
DO - 10.1097/00003081-200203000-00027
KW - Administration, Intravesical
Amitriptyline
Antidepressive Agents, Tricyclic
Autoimmunity
Capsaicin
Cystectomy
Cystitis, Interstitial
Dimethyl Sulfoxide
Humans
Pentosan Sulfuric Polyester
Solvents
Urinary Diversion
Urodynamics
amitriptyline
anesthetic agent
anticonvulsive agent
BCG vaccine
belladonna alkaloid
bupivacaine
capsaicin
carbamazepine
cimetidine
cystistat
dimethyl sulfoxide
gabapentin
gentamicin
heparin
hyaluronic acid
hydroxyzine
hydroxyzine embonate
lidocaine
narcotic agent
oxychlorosene
paroxetine
pentosan polysulfate
resiniferatoxin
serotonin uptake inhibitor
silver nitrate
triamcinolone
venlafaxine
wcs 90
autoimmunity
bladder distension
bladder epithelium
conference paper
constipation
cystectomy
diet restriction
dyspepsia
fatigue
heart palpitation
human
infection
inflammation
interstitial cystitis
laser surgery
liver toxicity
mast cell
neurogenic bladder
neuromodulation
pathophysiology
respiration depression
self care
sexual dysfunction
treatment planning
urinalysis
urinary diversion
urine retention
urticaria
weight gain
xerostomia
Cystitis
SP - 259-272
ST - Interstitial cystitis: A pathophysiology and treatment update
T2 - Clinical Obstetrics and Gynecology
TI - Interstitial cystitis: A pathophysiology and treatment update
0036193697&doi=10.1097%2f00003081-200203000-
00027&partnerID=40&md5=22c1d378958217de4e48082ee3713b6c
VL - 45
ID - 8997
ER -
TY - JOUR
AB - Hidradenitis suppurativa (HS) is a chronic, recurrent, and inflammatory skin
disease characterized by painful, deep-seated, nodules, abscesses, and sinus tracts
in sensitive areas of the body, including axillary, inguinal, and anogenital
regions. Antibiotics represent the first-line pharmacological treatment of HS
because of their anti-inflammatory properties and antimicrobial effects. This
narrative review summarizes the most significant current issues on the role of
systemic antibiotics in the management of HS, critically analyzing the main limits
of their use (antibiotic resistance and toxicity). Although, in the last decades,
several cytokines have been implicated in the pathomechanism of HS and the research
on the use of novel biologic agents in HS has been intensified, antibiotics remain
a valid therapeutic approach. Future challenges regarding antibiotic therapy in HS
comprise their use in association with biologics in the management of acute flare
or as a bridge therapy to surgery. © 2023 by the authors.
AN - rayyan-553780992
AU - Molinelli, E.
AU - De Simoni, E.
AU - Candelora, M.
AU - Sapigni, C.
AU - Brisigotti, V.
AU - Rizzetto, G.
AU - Offidani, A.
AU - Simonetti, O.
IS - 6
KW - antibiotic resistance
antibiotics
clindamycin
dalbavancin
ertapenem
guidelines
hidradenitis suppurativa rifampicin
tetracycline
adalimumab
ampicillin
antibiotic agent
azithromycin
biological product
C reactive protein
carbapenem
ceftriaxone
chloramphenicol
ciprofloxacin
clarithromycin
colchicine
colistin
corticosteroid
cytokine
daptomycin
DNA topoisomerase (ATP hydrolysing)
doxycycline
epidermal growth factor receptor
erythromycin
etretin
gelatinase B
interleukin 12
interleukin 17
interleukin 6
interleukin 8
lincosamide
linezolid
macrolide
meropenem
metronidazole
minocycline
moxifloxacin
oxazolidinone derivative
rifampicin
silver nanoparticle
teicoplanin
tigecycline
transcriptome
trimethoprim
vancomycin
vasculotropin
abscess
anemia
antibiotic therapy
antifungal activity
bacterial load
biofilm
body mass
bridging therapy
cholestasis
dermatitis
Dermatology Life Quality Index
disease activity
disease free survival
disk diffusion
dysbiosis
dyspepsia
erythema
headache
human
inflammation
latent tuberculosis
liver injury
methicillin susceptible Staphylococcus aureus
neurotoxicity
nonhuman
pain intensity
perineum
pharmacogenomics
practice guideline
protein synthesis
pruritus
pseudomembranous colitis
psoriasis
quality of life
Review
skin disease
Streptococcus pyogenes
suppurative hidradenitis
systemic therapy
Th17 cell
PY - 2023
ST - Systemic Antibiotic Therapy in Hidradenitis Suppurativa: A Review on
Treatment Landscape and Current Issues
T2 - Antibiotics
TI - Systemic Antibiotic Therapy in Hidradenitis Suppurativa: A Review on
Treatment Landscape and Current Issues
85163743020&doi=10.3390%2fantibiotics12060978&partnerID=40&md5=90a06b994ccda40b5912
ebf6521f8867
VL - 12
ID - 8998
ER -
TY - JOUR
AB - From earlier studies using amalgam of known physical and sealing properties
it was concluded that the initially high mercury content was the main reason for
pulpal changes and that clinical leakage had been less important. The purpose of
the present investigation was to study how a possible sealing insufficiency may
contribute to the pulpal changes beneath restorations of silver amalgam. The
influence of bacteria at the tooth/filling interface upon pulpal changes was also
studied. The material consisted of 21 contralateral pairs of premolars.
Intermediate depth cavities were prepared in 13 pairs and deep cavities in 8 pairs.
All cavities were filled with silver amalgam by the wet technique. The restoration
in one tooth of each pair was sealed off by glued metal foil protected by a
cemented orthodontic band. After 1 week the teeth were extracted and examined
histologically. In the pulp of teeth with restorations exposed to the oral
environment, the frequency of dilated capillaries and inflammatory cells in the
odontoblast - cell-rich zone boundary, associated with the dentinal tubules
involved in the cavity preparation, was insignificantly higher statistically than
in those with seal-off restorations. A few scattered bacteria were found on the
cavity wall in some cases. It was concluded that, in the short run, the pulp
reaction was influenced by sealing insufficiency, which may develop during the
experimental period as a result of external factors such as tooth deformation
and/or changes in temperature. No influence from bacteria on the cavity wall could
be established.
AN - rayyan-553782375
AU - Möller, B.
IS - 2
J2 - Swed Dent J
KW - Dental Amalgam/*toxicity
Dental Pulp/*drug effects/microbiology/pathology
Dental Restoration, Permanent/*standards
Humans
Pulpitis/*etiology/pathology
Humanities
Humanism
Dental Pulp
LA - eng
PY - 1979
SN - 0347-9994 (Print)
SP - 33-8
ST - Reaction of the human dental pulp to silver amalgam restorations. The effect
of enclosing amalgam of initially high plasticity in intermediate depth or deep
cavities
T2 - Swedish dental journal
TI - Reaction of the human dental pulp to silver amalgam restorations. The effect
of enclosing amalgam of initially high plasticity in intermediate depth or deep
cavities
VL - 3
ID - 10282
ER -
TY - JOUR
AB - Purpose: This article details the application of pristine nitrogen and sulfur
doped carbon quantum dots (CQDs) as a novel fluorescence biosensor for the
detection of glutathione. The second object of this study is to evaluate reduction
of cellular nitric oxide in microglial cells. Methods: Microwave assisted
hydrothermal method was used for the fabrication of CQDs. Unlike conventional
methods which utilize metallic or transition metal coating over CQDs for the
fabrication of fluorescence switch on/off probes, our simple yet efficient CQDs
itself performed as a biosensor that is both selective and sensitive towards
glutathione (GSH). Particle size analyzer, scanning electron microscope, atomic
force microscopy, high-performance X-ray photoelectron spectroscopy, fourier-
transform infrared spectroscopy were used for physicochemical characterization of
developed CQDs. Photoluminescence properties of CQDs were analyzed using
photoluminescence spectroscope for glutathione detection. Furthermore, microglial
cells were used to evaluate reduction of cellular nitric oxide. Results: The
developed biosensor was able to detect GSH within a short time of 2 min. Hemolysis
assay confirmed negligible red blood cell lysis even at a higher concentration of
0.2 mg/mL. Furthermore, the developed CQDs demonstrated enhanced cellular uptake,
which resulted in generating fluorescence from the BV-2 microglial
cells. Interestingly, the developed CQDs were able to mitigate the secretion of
toxic pro-inflammatory cytokine, nitric oxide (NO) from the lipopolysaccharide
(LPS) insulted BV-2 microglial cells. A 50% reduction in the secretion of NO was
observed after treating with CQDs in the LPS treated BV-2 cells. Conclusion: These
novel fluorescent CQDs with low manufacturing costs, high selectivity and
sensitivity towards GSH and shorter detection time manifest them as a promising
nanomaterial for diverse biomedical applications. © 2020, The Korean Society of
Pharmaceutical Sciences and Technology.
AN - rayyan-553780995
AU - Mondal, J.
AU - Revuri, V.
AU - Choochana, P.
AU - Ganesan, P.
AU - Kang, W. J.
AU - Lee, Y. K.
DO - 10.1007/s40005-019-00466-8
IS - 2
KW - Biosensor
Carbon quantum dots
Glutathione
Neuroinflammation
Nitric oxide
acetylcholine
carbon
cystamine
cystine
glutathione
gold nanoparticle
graphene oxide
lead
lipopolysaccharide
nitric oxide
nitrogen
quantum dot
silver nanoparticle
sulfur
zinc
Article
cell viability
controlled study
cytotoxicity
hemolysis assay
human
human cell
limit of detection
microglia
MTT assay
oxidation reduction potential
oxidative stress
particle size
photoluminescence
priority journal
spectral sensitivity
spectrofluorometry
X ray diffraction
PY - 2020
SP - 209-218
ST - Sulfur and nitrogen doped carbon quantum dots for detection of glutathione
and reduction of cellular nitric oxide in microglial cells
T2 - Journal of Pharmaceutical Investigation
TI - Sulfur and nitrogen doped carbon quantum dots for detection of glutathione
and reduction of cellular nitric oxide in microglial cells
85078160710&doi=10.1007%2fs40005-019-00466-
8&partnerID=40&md5=1faff647c476857a964ca16c42b1dd65
VL - 50
ID - 8999
ER -
TY - JOUR
AB - The aim of this study was to evaluate the antibacterial activity and
cytotoxic effect of the compound SIAB-GV3, a new formulation presenting as an
aqueous suspension of silicon dioxide (SiO2) functionalized with silver (Ag+)
nanoparticles and benzalkonium. The product is formulated as an adjuvant for the
treatment of inflammatory diseases of the oral cavity. This study demonstrates that
SIAB-GV3 possesses strong antimicrobial activity against most of the common oral
pathogens, in particular against Streptococcus pyogenes, Porphyromonas gingivalis
and Aggregatibacter actinimycetemcomitans. The cytotoxic effect against human
embryo lung fibroblasts (HELF cells) was evaluated at different times, from 1 h to
168 h, using concentrations of SIAB-GV3 ranging from 50 mg/ml to 0.0008 mg/ml. At
the concentration of 10 mg/ml, commonly used in clinical practice, the compound
results cytotoxic after about 2 hours, this time being much longer than the
typical time of local application, which is no more than 10 minutes. In conclusion,
our findings suggest that SIAB-GV3 has a good antibacterial activity against the
most common oral pathogens even at very low concentrations and a low cytotoxic
activity, thanks to the synergistic effects of Ag nanoparticles, silicon dioxide
and benzalkonium.
AN - rayyan-553782114
AU - Monzillo, V.
AU - Dalla Valle, C.
AU - Corbella, M.
AU - Percivalle, E.
AU - Sassera, D.
AU - Scevola, D.
AU - Marone, P.
IS - 4
J2 - New Microbiol
KW - Anti-Bacterial Agents/*pharmacology/toxicity
Bacteria/*drug effects
Cell Line
Fibroblasts/drug effects
Humans
Silicon Dioxide/pharmacology/toxicity
Silver/*pharmacology/toxicity
LA - eng
N1 - Department of Infectious Diseases, Fondazione IRCCS Policlinico San Matteo,
Pavia, Italy.
PY - 2014
SN - 1121-7138 (Print)
SP - 535-41
ST - Antibacterial activity and cytotoxic effect of SIAB-GV3
T2 - The new microbiologica
TI - Antibacterial activity and cytotoxic effect of SIAB-GV3
VL - 37
Y2 - 10
ID - 10025
ER -
TY - GEN
AB - Embora a terapia fotodinâmica venha sendo utilizada como uma ferramenta útil
nos últimos 30 anos em oncologia, poucos estudos clínicos em odontologia têm sido
conduzidos. A terapia fotodinâmica (TFD) utiliza fotossensibilizantes atóxicos e
seletivos que são administrados nas células alvo seguida de aplicação local de luz
visível, produzindo espécies reativas de oxigênio capazes de ocasionar morte
celular por apoptose ou necrose, de afetar a vascularidade local, além de exercer
importantes efeitos no sistema imune. Novas gerações de fármacos
fotossensibilizantes, como as ftalocianinas nanoparticuladas tem apresentado
excelentes resultados na atividade antitumoral e antibacteriana. Neste contexto, o
presente trabalho realizou o primeiro protocolo clínico de aplicação local da
nanoemulsão de cloroalumínio ftalocianina (AlClFc) seguida de irradiação em gengiva
de humanos, e analisou descritiva e comparativamente, por meio de imunoistoquímica,
a expressão de RANK, RANKL, OPG e VEGF em um modelo split-mouth. Oito voluntários
saudáveis com indicação clínica de exodontia foram incluídos no estudo. Sete dias
antes da exodontia, foi aplicado na gengiva dos participantes, 5µM de nanoemulsão
de AlClFc seguida de irradiação com laser diodo (660nm, 7J/cm2), o lado
contralateral foi utilizado como controle. Os espécimes teciduais foram removidos
sete dias após a TFD e subdivididos em dois grupos (grupo teste e grupo controle)
para análise histológica e imunoistoquímica. Os pacientes foram monitorados no dia
aplicação, 7, 14 e 30 dias após a terapia para avaliação de efeitos adversos da
terapia. Alterações vasculares foram observadas nas amostras gengivais que
receberam a TFD. Áreas de edema e congestão vascular, além de intensa
vascularização foram visualizadas. Adicionalmente, focos de calcificação distrófica
em região subepitelial foram visualizados nos espécimes do grupo teste. Os
resultados demonstraram um padrão similar dos escores de imunomarcação de RANK,
RANKL e VEGF entre os grupos teste e controle, não havendo diferença estatística
significante (p=0.317, p=0.777, p=0.814, respectivamente). RANK e RANKL exibiram
imunomarcação fraca ou ausente na maioria dos espécimes analisados. Não houve
imunomarcação para a OPG. O VEGF mostrou imunomarcação moderada a forte nos
espécimes do grupo teste. Adicionalmente, o estudo clínico mostrou que a terapia
foi bem tolerada por todos os pacientes. Os efeitos adversos foram de curta duração
e totalmente reversíveis. Tomados em conjunto, os resultados apresentados neste
estudo mostraram que o protocolo utilizado por nós, mediado por nanoemulsão
contendo AlClFc, é seguro para aplicação clínica em tecido gengival e, sugerem uma
forte imunomarcação para o VEGF após a terapia. (AU) Although photodynamic therapy
have been used as a useful tool over the past 30 years in oncology, few clinical
trials have been conducted in dentistry. Photodynamic therapy (PDT) uses non-toxic
photosensitizers and selective which are administered in target cells followed by
local application of visible light, producing reactive oxygen species capable of
causing cell death by apoptosis or necrosis, injured the local vasculature, and
exert important effects on the immune system. New generations of photosensitizing
agents, such as nanoparticulate phthalocyanines, has shown excellent results in
antitumor and antibacterial activity . In this context, the present work
constitutes the first clinical protocol of local application of nanoemulsion
chloro-aluminum phthalocyanine (AlClFc) followed by irradiation in human gingiva,
and analyzed descriptively and comparatively , by means of immunohistochemistry ,
the expression of RANK , RANKL , OPG and VEGF in a split -mouth model. Eight
healthy volunteers with clinical indication for extraction were included in the
study . Seven days before the extraction, was injected in the gingiva of
participants, 5µM of nanoemulsion AlClFc followed by irradiation with diode laser
(660nm, 7J/cm2 ), he contralateral side was used as control. Tissue specimens were
removed seven days after the TFD is performed. Tissues sample were divided into two
groups (test and control groups) for histological and immunohistochemical analysis.
Patients were monitored at days, 0, 7, 14 and 30 to assess adverse effects of the
therapy. Vascular alterations were seen in gingival samples that received PDT.
Areas of edema and vascular congestion, and intense vascularization were viewed .
Additionally, dystrophic calcification in subepithelial region were observed in the
test group. The results showed a similar pattern of immunostaining scores of RANK,
RANKL and VEGF between the test and control groups, with no statistically
significant difference (p = 0.317, p = 0.777, p = 0.814, respectively). RANK and
RANKL exhibited weak or absent immunostaining in most specimens analyzed. There was
no immunostaining for OPG. VEGF showed moderate to strong immunostaining in
specimens from the test group. In addition, the clinical study showed that therapy
was well tolerated by all patients. Adverse effects were short-time and completely
reversible. Taken together, the results presented in this study showed that PDT
mediated by nanoemulsion containing AlClPc is safe for clinical application in
gingival tissue and suggests that a strong immunostaining for VEGF after therapy.
(AU)
AN - rayyan-553781000
AU - Moraes, Maiara de
KW - Fator A de crescimento do endotélio vascular
Fotoquimioterapia
Fármacos fotossensibilizantes
Ligante RANK
Osteoprotegerin
Osteoprotegerina
Photochemotherapy
Photosensitizing agents
RANK ligand
Vascular endothelial growth factor A
LA - pt
PY - 2014
SP - 128-128
ST - Análise imunoistoquímica após terapia fotodinâmica com cloro-alumínio
ftalocianina em tecidos gengivais de humanos
TI - Análise imunoistoquímica após terapia fotodinâmica com cloro-alumínio
ftalocianina em tecidos gengivais de humanos
UR - https://pesquisa.bvsalud.org/portal/resource/pt/biblio-867010
Y2 - 8 y3 - 1
ID - 9000
ER -
TY - JOUR
AB - Elevated levels of corticosteroids and stress play key roles in the
pathophysiology of affective disorders. Corticosterone (CORT)-treated rats have
emerged as a pharmacological model of depression-like behaviors. Previous studies
have shown that CORT administration induces neuronal atrophy in the CA3 subfield of
the hippocampus and laminae II/III of the prefrontal cortex. However, little
attention has been given to other limbic structures such as the amygdala and the
nucleus accumbens (NAcc). We investigated here whether 3 weeks of CORT
administration in rats causes dendritic remodeling and spine density reorganization
in the basolateral amygdala and pyramidal neurons of the CA1 subfield of the
hippocampus as well as in spiny medium neurons of NAcc. Quantitative morphological
analysis revealed retracted neuronal arborizations and modified configuration of
length depending on branch order in medium spiny neurons of the NAcc of CORT-
treated animals. Moreover, distal dendritic sections of the NAcc showed massive
reductions in the number of spines caused by the CORT treatment. This treatment
also induced a reduction in total dendritic length specific to fourth and sixth
branch orders of pyramidal CA1 hippocampal neurons. These neurons also showed
decreased branching and diminished number of spines. Finally, pyramidal neurons of
the basolateral amygdala were apparently not significantly affected by the CORT
treatment. Taken together, these data show for the first time neuronal
morphological alterations in the NAcc in the CORT model of depression-like
behaviors. Our results also add further information about the morphological
reorganization occurring in CORT-sensitive regions of the limbic system.
AN - rayyan-553782244
AU - Morales-Medina, J. C.
AU - Sanchez, F.
AU - Flores, G.
AU - Dumont, Y.
AU - Quirion, R.
DO - 10.1016/j.jchemneu.2009.05.009
IS - 4
J2 - J Chem Neuroanat
KW - Amygdala/drug effects/metabolism/pathology
Animals
Anti-Inflammatory Agents/metabolism/toxicity
Cell Shape/drug effects/physiology
Corticosterone/metabolism/*toxicity
Dendrites/drug effects/metabolism/pathology
Dendritic Spines/drug effects/metabolism/pathology
Drug Administration Schedule
Hippocampus/drug effects/metabolism/pathology
Limbic System/drug effects/metabolism/*pathology
Male
Neural Pathways/drug effects/metabolism/pathology
Neuronal Plasticity/drug effects/*physiology
Neurons/drug effects/metabolism/*pathology
Nucleus Accumbens/drug effects/metabolism/pathology
Pyramidal Cells/drug effects/metabolism/pathology
Rats
Silver Staining
Stress, Psychological/metabolism/*pathology/physiopathology
Nucleus Accumbens
Hippocampus
Amygdala
LA - eng
N1 - Dept of Neurology & Neurosurgery, McGill University, Montréal, QC, Canada.
PY - 2009
SP - 266-72
ST - Morphological reorganization after repeated corticosterone administration in
the hippocampus, nucleus accumbens and amygdala in the rat
T2 - Journal of chemical neuroanatomy
TI - Morphological reorganization after repeated corticosterone administration in
the hippocampus, nucleus accumbens and amygdala in the rat
VL - 38
Y2 - 12
ID - 10154
ER -
TY - JOUR
AB - Bacterial endotoxins are a component of particulate matter (PM) with
anticipated health implications, yet we know little about how host reception of
endotoxin through toll-like receptor 4 (TLR4) is affected by its association with
other PM components. Subsequently, we investigated the relationship between
endotoxin concentration (recombinant Factor C (rFC) assay) and host recognition
(HEK Blue-TLR4 NF-kB reporter cell line based assay) in various compositions of
urban PM, including road traffic, industrial and urban green land use classes.
While the assays did not correlate strongly between each other, the TLR4 reporter
cell line was found to be better correlated to the IL-8 response of PM.
Furthermore, the ability of the quantified endotoxin (rFC assay) to stimulate the
TLR4/MD-2 complex was significantly affected by the urban land use class, where
traffic locations were found to be significantly higher in bioactive endotoxin than
the industrial and green locations. We subsequently turned our attention to PM
composition and characterized the samples based on transition metal content
(through ICP-MS). The effect of nickel and cobalt – previously reported to activate
the hTLR4/MD-2 complex – was found to be negligible in comparison to that of iron.
Here, the addition of iron as a factor significantly improved the regression model
between the two endotoxin assays, explaining 77% of the variation of the TLR4
stimulation and excluding the significant effect of land use class. Moreover, the
effect of iron proved to be more than a correlation, since dosing LPS with Fe2+ led
to an increase up to 64% in TLR4 stimulation, while Fe2+ without LPS was unable to
stimulate a response. This study shows that endotoxin quantification assays (such
as the rFC assay) may not always correspond to human biological recognition of
endotoxin in urban PM, while its toxicity can be synergistically influenced by the
associated PM composition. Although endotoxins are typically quantified in ambient
air, its toxicity is significantly influenced by the associated PM composition
(e.g. as shown in this study with iron, one of the most abundant transition metals
found in urban PM). © 2018
AN - rayyan-553781002
AU - Moretti, S.
AU - Smets, W.
AU - Hofman, J.
AU - Mubiana, K. V.
AU - Oerlemans, E.
AU - Vandenheuvel, D.
AU - Samson, R.
AU - Blust, R.
AU - Lebeer, S.
DO - 10.1016/j.envpol.2018.09.148
KW - Inflammation
Lipopolysaccharide
Particulate matter
TLR4
Transition metals
Biological Assay
Cell Line
Endotoxins
Humans
Interleukin-8
Metals
Particulate Matter
Transition Elements
Cell culture
Iron
Land use
Regression analysis
Toxicity
aluminum
arsenic
cadmium
calcium
chloride
chromium
cobalt
copper
endotoxin
interleukin 8
iron
lead
lipopolysaccharide
manganese
nickel
protein MD 2
silver
transition element
zinc
metal
TLR4 protein, human
Bacterial endotoxins
Biological recognition
Lipopolysaccharides
Quantification assays
bacterium
particulate matter
polysaccharide
protein
toxin
Article
cell assay
human
industrial area
land use
traffic
bioassay
cell line
drug effect
metabolism
statistics and numerical data
toxicity
Assays
Humanities
Humanism
PY - 2019
SP - 118-126
ST - Human inflammatory response of endotoxin affected by particulate matter-bound
transition metals
T2 - Environmental Pollution
TI - Human inflammatory response of endotoxin affected by particulate matter-bound
transition metals
85054748150&doi=10.1016%2fj.envpol.2018.09.148&partnerID=40&md5=e602dc4a5dad70aa5ba
a59e7bd782b81
VL - 244
ID - 9001
ER -
TY - JOUR
AB - Nanoparticles can interact with the complement system and modulate the
inflammatory response. The effect of these interactions on the complement activity
strongly depends on physicochemical properties of nanoparticles. The interactions
of silver nanoparticles with serum proteins (particularly with the complement
system components) have the potential to significantly affect the antibacterial
activity of serum, with serious implications for human health. The aim of the study
was to assess the influence of graphite oxide (GO) nanocomposites (GO, GO‐
PcZr(Lys)2‐Ag, GO‐Ag, GO‐PcZr(Lys)2) on the antibacterial activity of normal human
serum (NHS), serum activity against bacteria isolated from alveoli treated with
nanocomposites, and nanocomposite sensitivity of bacteria exposed to serum in vitro
(using normal human serum). Additionally, the in vivo cytotoxic effect of the GO
compounds was determined with application of a Galleria mellonella larvae model.
GO‐PcZr(Lys)2, without IR irradiation enhance the antimicrobial efficacy of the
human serum. IR irradiation enhances bactericidal activity of serum in the case of
the GO‐PcZr(Lys)2‐Ag sample. Bacteria exposed to nanocomposites become more
sensitive to the action of serum. Bacteria exposed to serum become more sensitive
to the GO‐Ag sample. None of the tested GO nanocomposites displayed a cytotoxicity
towards larvae. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553781004
AU - Morka, K. D.
AU - Wernecki, M.
AU - Kędziora, A.
AU - Książczyk, M.
AU - Dudek, B.
AU - Gerasymchuk, Y.
AU - Lukowiak, A.
AU - Bystroń, J.
AU - Bugla‐płoskońska, G.
DO - 10.3390/ijms22147386
IS - 14
KW - Bactericidal action of serum
E. coli
GO nanocomposites
Photoactivity
Animals
Cell Survival
Escherichia coli
Graphite
Humans
Infrared Rays
Larva
Lepidoptera
Metal Nanoparticles
Nanocomposites
Oxides
Serum
Silver
graphite
nanocomposite
oxide
phthalocyanine
silver nanoparticle
zirconium derivative
antiinfective agent
metal nanoparticle
silver
animal experiment
Article
bacterial survival
controlled study
cytotoxicity
Galleria mellonella
human
in vitro study
in vivo study
larva
lung alveolus
nonhuman
serum bactericidal activity
animal
cell survival
chemistry
drug effect
infrared radiation
microbiology
radiation response
serum
PY - 2021
ST - The impact of graphite oxide nanocomposites on the antibacterial activity of
serum
TI - The impact of graphite oxide nanocomposites on the antibacterial activity of
serum
85109333277&doi=10.3390%2fijms22147386&partnerID=40&md5=359defe7bcff01b0fb5403d7322
22d97
VL - 22
ID - 9002
ER -
TY - JOUR
AB - In this study, we explored the effects of a longer term application, up to
12 weeks, of photobiomodulation in normal, naïve macaque monkeys. Monkeys (n = 5)
were implanted intracranially with an optical fibre device delivering
photobiomodulation (red light, 670 nm) to a midline midbrain region. Animals were
then aldehyde-fixed and their brains were processed for immunohistochemistry. In
general, our results showed that longer term intracranial application of
photobiomodulation had no adverse effects on the surrounding brain parenchyma or on
the nearby dopaminergic cell system. We found no evidence for photobiomodulation
generating an inflammatory glial response or neuronal degeneration near the implant
site; further, photobiomodulation did not induce an abnormal activation or
mitochondrial stress in nearby cells, nor did it cause an abnormal arrangement of
the surrounding vasculature (endothelial basement membrane). Finally, because of
our interest in Parkinson’s disease, we noted that photobiomodulation had no impact
on the number of midbrain dopaminergic cells and the density of their terminations
in the striatum. In summary, we found no histological basis for any major biosafety
concerns associated with photobiomodulation delivered by our intracranial approach
and our findings set a key template for progress onto clinical trial on patients
with Parkinson’s disease. © 2017, Springer-Verlag GmbH Germany.
AN - rayyan-553781005
AU - Moro, C.
AU - Torres, N.
AU - Arvanitakis, K.
AU - Cullen, K.
AU - Chabrol, C.
AU - Agay, D.
AU - Darlot, F.
AU - Benabid, A. L.
AU - Mitrofanis, J.
DO - 10.1007/s00221-017-5048-7
IS - 10
KW - 670 nm
Behaviour
Macaque monkeys
Striatum
Substantia nigra
Tyrosine hydroxylase
Animals
Corpus Striatum
Dopaminergic Neurons
Low-Level Light Therapy
Macaca fascicularis
Mesencephalon
Optical Fibers
chaperonin 60
IBA1 protein
protein
protein fos
unclassified drug
glass fiber
adult
animal cell
animal tissue
antibody labeling
Article
basement membrane
cell damage
controlled study
corpus striatum
cytoarchitecture
endothelial basement membrane
immunoreactivity
mesencephalon
microglia
nerve cell degeneration
Nissl staining
nonhuman
parenchyma
Parkinson disease
photobiomodulation
priority journal
silver impregnation
thalamus
adverse device effect
animal
devices
dopaminergic nerve cell
Humanities
Humanism
Humans
Primates
PY - 2017
SP - 3081-3092
ST - No evidence for toxicity after long-term photobiomodulation in normal non-
human primates
T2 - Experimental Brain Research
TI - No evidence for toxicity after long-term photobiomodulation in normal non-
human primates
85025812162&doi=10.1007%2fs00221-017-5048-
7&partnerID=40&md5=afa24af1b72ce9ac938d5cdcc0ac6246
VL - 235
ID - 9003
ER -
TY - JOUR
AB - Background: Nanosilver possesses antiviral, antibacterial, anti-inflammatory,
anti-angio-genesis, antiplatelet, and anticancer properties. The development of
disinfectants, inactivated vac-cines, and combined etiotropic and immunomodulation
therapy against respiratory viral infections, including COVID-19, remains urgent.
Aim: Our goal was to determine the SARS-CoV-2 molecular targets (genomic RNA and
the structural virion proteins S and N) for silver-containing nanomateri-als.
Methods: SARS-CoV-2 gene cloning, purification of S2 and N recombinant proteins,
viral RNA isolation from patients’ blood samples, reverse transcription with
quantitative real-time PCR ((RT)2-PCR), ELISA, and multiplex immunofluorescent
analysis with magnetic beads (xMAP) for detection of 17 inflammation markers.
Results: Fluorescent Ag nanoclusters (NCs) less than 2 nm with a few recovered
silver atoms, citrate coated Ag nanoparticles (NPs) with diameters of 20–120 nm,
and nanoconjugates of 50–150 nm consisting of Ag NPs with different protein
envelopes were con-structed from AgNO3 and analyzed by means of transmission
electron microscopy (TEM), atomic force microscopy (AFM), ultraviolet-visible light
absorption, and fluorescent spectroscopy. SARS-CoV-2 RNA isolated from COVID-19
patients’ blood samples was completely cleaved with the artificial RNase complex
compound Li+[Ag+2Cys2−(OH−)2(NH3)2] (Ag-2S), whereas other Ag-contain-ing materials
provided partial RNA degradation only. Treatment of the SARS-CoV-2 S2 and N re-
combinant antigens with AgNO3 and Ag NPs inhibited their binding with specific
polyclonal anti-bodies, as shown by ELISA. Fluorescent Ag NCs with albumin or
immunoglobulins, Ag-2S com-plex, and nanoconjugates of Ag NPs with protein shells
had no effect on the interaction between coronavirus recombinant antigens and
antibodies. Reduced production of a majority of the 17 inflammation biomarkers
after treatment of three human cell lines with nanosilver was demonstrated by xMAP.
Conclusion: The antiviral properties of the silver nanomaterials against SARS-CoV-2
coronavirus differed. The small-molecular-weight artificial RNase Ag-2Sprovided
exhaustive RNA destruction but could not bind with the SARS-CoV-2 recombinant
antigens. On the contrary, Ag+ ions and Ag NPs interacted with the SARS-CoV-2
recombinant antigens N and S but were less efficient at performing viral RNA
cleavage. One should note that SARS-CoV-2 RNA was more stable than MS2 phage RNA.
The isolated RNA of both the MS2 phage and SARS-CoV-2 were more de-gradable than
the MS2 phage and coronavirus particles in patients’ blood, due to the protection
with structural proteins. To reduce the risk of the virus resistance, a combined
treatment of Ag-2S with Ag NPs could be used. To prevent cytokine storm during the
early stages of respiratory infections with RNA-containing viruses, nanoconjugates
of Ag NPs with surface proteins could be used. © 2022 by the authors. Licensee
AN - rayyan-553781010
AU - Morozova, O. V.
AU - Manuvera, V. A.
AU - Grishchechkin, A. E.
AU - Barinov, N. A.
AU - Shevlyagina, N. V.
AU - Zhukhovitsky, V. G.
AU - Lazarev, V. N.
AU - Klinov, D. V.
DO - 10.3390/v14050902
IS - 5
KW - beta-coronavirus
ELISA
nanosilver
RNA-containing bacteriophage MS2
RT2-PCR
xMAP
Antiviral Agents
Cations
COVID-19
Cystine
Humans
Inflammation
Metal Nanoparticles
Nanoconjugates
Ribonucleases
RNA, Viral
SARS-CoV-2
Silver
Virion
coronavirus nucleocapsid protein
gamma interferon
interleukin 10
interleukin 12p70
interleukin 13
interleukin 17
interleukin 1beta
interleukin 2
interleukin 4
interleukin 5
interleukin 6
interleukin 7
interleukin 8
metal complex
nanoconjugate
silver
silver cystine complex
silver nanocluster
silver nanoparticle
silver nitrate
unclassified drug
virus RNA
vitronectin
antivirus agent
cation
cystine
metal nanoparticle
recombinant protein
ribonuclease
adult
analytic method
Article
controlled study
cytokine storm
cytotoxicity
female
fluorescent spectroscopy
HEp-2 cell line
HT-29 cell line
human
human cell
human tissue
immunofluorescence
major clinical study
male
molecular cloning
MTT assay
multiplex immunofluorescence
nasopharyngeal swab
particle size
plasmid
protein purification
real time reverse transcription polymerase chain reaction
RNA isolation
Sanger sequencing
virion
chemistry
genetics
inflammation
PY - 2022
ST - Targeting of Silver Cations, Silver-Cystine Complexes, Ag Nanoclusters, and
Nanoparticles towards SARS-CoV-2 RNA and Recombinant Virion Proteins
T2 - Viruses
TI - Targeting of Silver Cations, Silver-Cystine Complexes, Ag Nanoclusters, and
Nanoparticles towards SARS-CoV-2 RNA and Recombinant Virion Proteins
85129859965&doi=10.3390%2fv14050902&partnerID=40&md5=56109e4ea70eceb17ce216bc79b986
aa
VL - 14
ID - 9005
ER -
TY - JOUR
AB - E-cigarettes utilize a wide range of flavoring chemicals with respiratory
health effects that are not well understood. In this study, we used pulmonary-
associated cell lines to assess the in vitro cytotoxic effects of 30 flavoring
chemicals. Human bronchial epithelial cells (BEAS-2B) and both naive and activated
macrophages (THP-1) were treated with 10, 100, and 1000 mu M of flavoring chemicals
and analyzed for changes in viability, cell membrane damage, reactive oxygen
species (ROS) production, and inflammatory cytokine release. Viability was
unaffected for all chemicals at the 10 and 100 mu M concentrations. At 1000 mu M,
the greatest reductions in viability were seen with decanal, hexanal, nonanal,
cinnamaldehyde, eugenol, vanillin, alpha-pinene, and limonene. High amounts of ROS
were elicited by vanillin, ethyl maltol, and the diketones (2,3-pentanedione, 2,3-
heptanedione, and 2,3-hexanedione) from both cell lines. Naive THP-1 cells produced
significantly elevated levels of IL-1 beta, IL-8, and TNF-alpha when exposed to
ethyl maltol and hexanal. Activated THP-1 cells released increased IL-1 beta and
TNF-alpha when exposed to ethyl maltol, but many flavoring chemicals had an
apparent suppressive effect on inflammatory cytokines released by activated
macrophages, some with varying degrees of accompanying cytotoxicity. The diketones,
L-carvone, and linalool suppressed cytokine release in the absence of cytotoxicity.
These findings provide insight into lung cell cytotoxicity and inflammatory
cytokine release in response to flavorings commonly used in e-cigarettes.
AN - rayyan-553781012
AU - Morris, A. M.
AU - Leonard, S. S.
AU - Fowles, J. R.
AU - Boots, T. E.
AU - Mnatsakanova, A.
AU - Attfield, K. R.
DO - 10.3390/ijerph182111107
IS - 21
KW - Humanities
Humanism
Humans
Epithelial Cells
PY - 2021
SN - 1660-4601
ST - Effects of E-Cigarette Flavoring Chemicals on Human Macrophages and Bronchial
Epithelial Cells
TI - Effects of E-Cigarette Flavoring Chemicals on Human Macrophages and Bronchial
Epithelial Cells
VL - 18
Y2 - 11
ID - 9006
ER -
TY - JOUR
AB - Respiratory syncytial virus (RSV) is an important etiological agent of
respiratory infection in children for which no specific treatment option is
available. The RSV virion contains two surface glycoproteins (F and G) that are
vital for the initial phases of infection, making them critical targets for RSV
therapeutics. Recent studies have identified the broad-spectrum antiviral
properties of silver nanoparticles (AgNPs) against respiratory pathogens, such as
adenovirus, parainfluenza, and influenza. AgNPs achieve this by attaching to viral
glycoproteins, blocking entry into the host cell. The objective of this study was
to evaluate the antiviral and immunomodulatory effects of AgNPs in RSV infection.
Herein we demonstrate AgNP-mediated reduction in RSV replication, both in
epithelial cell lines and in experimentally infected BALB/c mice. Marked reduction
in pro-inflammatory cytokines (i.e., IL-1α, IL-6, TNF-α) and pro-inflammatory
chemokines (i.e., CCL2, CCL3, CCL5) was also observed. Conversely, CXCL1, G-CSF,
and GM-CSF were increased in RSV-infected mice treated with AgNPs, consistent with
an increase of neutrophil recruitment and activation in the lung tissue. Following
experimental antibody-dependent depletion of neutrophils, the antiviral effect of
AgNPs in mice treated was ablated. To our knowledge, this is the first in vivo
report demonstrating antiviral activity of AgNPs during RSV infection. © 2019 by
the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553781014
AU - Morris, D.
AU - Ansar, M.
AU - Speshock, J.
AU - Ivanciuc, T.
AU - Qu, Y.
AU - Casola, A.
AU - Garofalo, R.
DO - 10.3390/v11080732
IS - 8
KW - AgNP
Anti-Ly6G
Antiviral
Epithelial cells
G-CSF
GM-CSF
KC
Neutrophils
Respiratory syncytial virus
Animals
Antiviral Agents
Cell Line
Cytokines
Epithelial Cells
Female
Humans
Immunologic Factors
Leukocyte Count
Lung
Metal Nanoparticles
Mice
Neutrophil Infiltration
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Human
Silver
Virus Replication
alpha interferon
beta interferon
CXCL1 chemokine
elastase
interleukin 10
interleukin 12p40
interleukin 12p70
interleukin 13
interleukin 1alpha
interleukin 6
interleukin 8
interleukin 9
povidone
protein
RANTES
silver nanoparticle
antivirus agent
cytokine
immunologic factor
metal nanoparticle
silver
A-549 cell line
airway obstruction
animal experiment
animal model
animal tissue
antiviral activity
Article
body plethysmography
body weight loss
colorimetry
controlled study
cytotoxicity test
enzyme activity
female
HEp-2 cell line
human
human cell
immunomodulation
leukocyte activation
mouse
neutrophil
nonhuman
peak expiratory flow
peak inspiratory flow
respiratory syncytial virus infection
viral plaque assay
virus replication
animal
cell line
chemistry
cytology
disease model
drug effect
epithelium cell
Human respiratory syncytial virus
immunology
leukocyte count
lung
metabolism
pathology
virology
PY - 2019
ST - Antiviral and immunomodulatory activity of silver nanoparticles in
experimental rsv infection
T2 - Viruses
TI - Antiviral and immunomodulatory activity of silver nanoparticles in
experimental rsv infection
85070476559&doi=10.3390%2fv11080732&partnerID=40&md5=c6c9b588c7cd8465fe017350c1c423
4b
VL - 11
ID - 9007
ER -
TY - JOUR
AB - Nanotechnology is a growing science that may provide several new applications
for medicine, food preservation, diagnostic technologies, and sanitation. Despite
its beneficial applications, there are several questions related to the safety of
nanomaterials for human use. The development of nanotechnology is associated with
some concerns because of the increased risk of carcinogenesis following exposure to
nanomaterials. The increased levels of reactive oxygen species (ROS) that are due
to exposure to nanoparticles (NPs) are primarily responsible for the genotoxicity
of metal NPs. Not all, but most metal NPs are able to directly produce free
radicals through the release of metal ions and through interactions with water
molecules. Furthermore, the increased production of free radicals and the cell
death caused by metal NPs can stimulate reduction/oxidation (redox) reactions,
leading to the continuous endogenous production of ROS in a positive feedback loop.
The overexpression of inflammatory mediators, such as NF-kB and STATs, the
mitochondrial malfunction and the increased intracellular calcium levels mediate
the chronic oxidative stress that occurs after exposure to metal NPs. In this
paper, we review the genotoxicity of different types of metal NPs and the redox
mechanisms that amplify the toxicity of these NPs. © 2019
AN - rayyan-553781017
AU - Mortezaee, K.
AU - Najafi, M.
AU - Samadian, H.
AU - Barabadi, H.
AU - Azarnezhad, A.
AU - Ahmadi, A.
DO - 10.1016/j.cbi.2019.108814
KW - DNA damage
Genotoxicity
Metal nanoparticles
Nanotechnology
Oxidative stress
Redox
Animals
Calcium
DNA Damage
Food Additives
Humans
Metal Nanoparticles
Neoplasms
Oxidation-Reduction
Oxidative Stress
aluminum nanoparticle
cobalt nanoparticle
copper nanoparticle
cosmetic
food additive
gold nanoparticle
iron nanoparticle
nanoparticle
nickel nanoparticle
palladium nanoparticle
silver nanoparticle
unclassified drug
calcium
metal nanoparticle
antifungal activity
calcium homeostasis
cancer risk
cancer therapy
carcinogenesis
cytotoxicity
genotoxicity
human
inflammation
ion transport
lotion
molecular imaging
nonhuman
oxidation reduction reaction
Review
teratogenesis
animal
chemistry
drug effect
metabolism
neoplasm
oxidative stress
Metals
PY - 2019
ST - Redox interactions and genotoxicity of metal-based nanoparticles: A
comprehensive review
TI - Redox interactions and genotoxicity of metal-based nanoparticles: A
comprehensive review
85071976190&doi=10.1016%2fj.cbi.2019.108814&partnerID=40&md5=98b7bd457c778d2f2d05d2
782ecead36
VL - 312
ID - 9008
ER -
TY - JOUR
AB - Hydroxyapatite has been extensively used in tissue engineering due to its
osteogenic potency, but its present toxicological facts are relatively
insufficient. Here, the possible gastric toxicity of hydroxyapatite nanoparticles
was evaluated biochemically to determine oxidant and antioxidant parameters in
rats' stomach tissues. At results, hydroxyapatite nanoparticles have declined
stomach antioxidant enzymes and reduced glutathione level, while an induction in
lipid peroxidation and nitric oxide has been observed. Furthermore, DNA oxidation
was analyzed by the suppression of toll-like receptors 2, nuclear factor-kappa B
and Forkhead box P3 gene expression and also 8-Oxo-2'-deoxyguanosine level as a
genotoxicity indicator. Various pro-inflammatory gene products have been identified
that intercede a vital role in proliferation and apoptosis suppression, among these
products: tumor suppressor p53, tumor necrosis factor-alpha and interliukin-6.
Moreover, the hydroxyapatite-treated group revealed wide histological alterations
and significant elevation in the number of proliferating cell nuclear antigen-
positive cells, which has been observed in the mucosal layer of the small
intestine, and these alterations are an indication of small intestine injury, while
the appearance of chitosan and curcumin nanoparticles in the combination group
showed improvement in all the above parameters with inhibition of toxic-oxidant
parameters and activation of antioxidant parameters.
AN - rayyan-553781019
AU - Mosa, I. F.
AU - Abd, H. H.
AU - Abuzreda, A.
AU - Assaf, N.
AU - Yousif, A. B.
DO - 10.1093/toxres/tfaa054
IS - 4
KW - Rats
Durapatite
PY - 2020
SN - 2045-452X 2045-4538
SP - 493-508
ST - Bio-evaluation of the role of chitosan and curcumin nanoparticles in
ameliorating genotoxicity and inflammatory responses in rats' gastric tissue
followed hydroxyapatite nanoparticles' oral uptake
T2 - TOXICOLOGY RESEARCH
TI - Bio-evaluation of the role of chitosan and curcumin nanoparticles in
ameliorating genotoxicity and inflammatory responses in rats' gastric tissue
followed hydroxyapatite nanoparticles' oral uptake
VL - 9
Y2 - 7
ID - 9009
ER -
TY - JOUR
AB - This study was designed to investigate the effectiveness of hybridized,
three-dimensional (3D) collagen implants in repair of experimentally-induced tendon
defects in rabbits. Seventy-five mature New Zealand albino rabbits were divided
into treated (n = 50) and control (n = 20) groups. The left Achilles tendon was
completely transected and 2 cm excised. In treated animals defects were filled with
hybridized collagen implants and repaired with sutures. In control rabbits tendon
defects were sutured similarly but the gap was left untreated. Changes in injured
and normal contralateral tendons were assessed weekly by ultrasonography. Among the
treated animals, small pilot groups were euthanized at 5, 10, 15, 20, 30, 40 (n = 5
at each time interval) and the remainder (n = 20) at 60 days post-injury. All control
animals were euthanized at 60 days. Tendon lesions of all animals were examined
morphologically and histologically immediately after death. Those of the
experimental groups (n = 20 for each) were examined for gross pathological,
histopathological and ultrastructural changes together with dry matter content at
60 days post-injury, as were the normal, contralateral tendons of both groups. In
comparison with healing lesions of control animals, the treated tendons showed
greater numbers of mature tenoblasts and tenocytes, minimal peritendinous adhesions
and oedema, together with greater echogenicity, homogeneity and fibril alignment.
Fewer chronic inflammatory cells were present in treated than control tendons.
Hybridized collagen implants acted as scaffolds for tenoblasts and longitudinally-
orientated newly-formed collagen fibrils, which encouraged tendon repair with
homogeneous, well-organized highly aligned scar tissue that was histologically and
ultrastructurally more mature than in untreated controls. Copyright © 2016 John
Wiley & Sons, Ltd.
AN - rayyan-553782377
AU - Moshiri, A.
AU - Oryan, A.
AU - Meimandi-Parizi, A.
AU - Silver, I. A.
AU - Tanideh, N.
AU - Golestani, N.
DO - 10.1002/term.1740
IS - 6
J2 - J Tissue Eng Regen Med
KW - *Absorbable Implants
Animals
*Bioprosthesis
Nanostructures/*chemistry
Rabbits
*Tendon Injuries/metabolism/pathology/surgery
Tendons/*metabolism/pathology
Tissue Scaffolds/*chemistry
Collagen
Tendons
Tendinopathy
LA - eng
N1 - Division of Surgery and Radiology, Department of Clinical Sciences, School of
Veterinary Medicine, Shiraz University, Iran.; Department of Pathology, School of
Veterinary Medicine, Shiraz University, Iran.; Division of Surgery and Radiology,
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University,
Iran.; Centre for Comparative and Clinical Anatomy, School of Veterinary Science,
Southwell Street, Bristol, UK.; Centre for Stem Cell Research and Transgenic
Animals, Shiraz University of Medical Sciences, Iran.; Division of Surgery and
Radiology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz
University, Iran.
PY - 2016
SP - 451-65
ST - Effectiveness of hybridized nano- and microstructure biodegradable,
biocompatible, collagen-based, three-dimensional bioimplants in repair of a large
tendon-defect model in rabbits
T2 - Journal of tissue engineering and regenerative medicine
TI - Effectiveness of hybridized nano- and microstructure biodegradable,
biocompatible, collagen-based, three-dimensional bioimplants in repair of a large
tendon-defect model in rabbits
VL - 10
Y2 - 6
ID - 10284
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are among the most widely used metal-based
nanomaterials (NMs) and their applications in different products, also as
antibacterial additives, are increasing. In the present manuscript, according to an
adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly
developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and
AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and
coated with polyvinylpyrrolidone-PVP). Cell viability, inflammatory response,
reactive oxygen species, oxidative DNA damage, cell cycle, and cell-particle
interactions were analyzed in the alveolar in vitro model, A549 cells. The results
show a different toxicity pattern of the novel Ag NPs compared to reference NPs and
that between the two novel NPs, the AgHEC solution is the one with the lower
toxicity and to be further developed within the SbD framework.
AN - rayyan-553781025
AU - Motta, G.
AU - Gualtieri, M.
AU - Saibene, M.
AU - Bengalli, R.
AU - Brigliadori, A.
AU - Carriere, M.
AU - Mantecca, P.
DO - 10.3390/toxics11020195
IS - 2
PY - 2023
SN - 2305-6304
ST - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
Pathway-Driven Approach on Different Silver Nanoparticles: Assessment of Acute
Responses in A549 Cells
T2 - TOXICS
TI - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
VL - 11
Y2 - 2
ID - 9012
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are among the most widely used metal-based
nanomaterials (NMs) and their applications in different products, also as
antibacterial additives, are increasing. In the present manuscript, according to an
adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly
developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and
AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and
coated with polyvinylpyrrolidone-PVP). Cell viability, inflammatory response,
reactive oxygen species, oxidative DNA damage, cell cycle, and cell-particle
interactions were analyzed in the alveolar in vitro model, A549 cells. The results
show a different toxicity pattern of the novel Ag NPs compared to reference NPs and
that between the two novel NPs, the AgHEC solution is the one with the lower
toxicity and to be further developed within the SbD framework.
AN - rayyan-553781988
AU - Motta, G.
AU - Gualtieri, M.
AU - Saibene, M.
AU - Bengalli, R.
AU - Brigliadori, A.
AU - Carrière, M.
AU - Mantecca, P.
DO - 10.3390/toxics11020195
IS - 2
J2 - Toxics
LA - eng
N1 - Department of Biotechnology and Biosciences, University of Milano-Bicocca,
Piazza della Scienza 2, 20126 Milan, Italy.; Research Centre POLARIS, Department of
Earth and Environmental Sciences, University of Milano-Bicocca, 20126 Milan,
Italy.; Research Centre POLARIS, Department of Earth and Environmental Sciences,
University of Milano-Bicocca, 20126 Milan, Italy.; Department of Earth and
Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126
Milan, Italy.; Research Centre POLARIS, Department of Earth and Environmental
Sciences, University of Milano-Bicocca, 20126 Milan, Italy.; Department of Earth
and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1,
20126 Milan, Italy.; Research Centre POLARIS, Department of Earth and Environmental
Sciences, University of Milano-Bicocca, 20126 Milan, Italy.; Department of Earth
and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1,
20126 Milan, Italy.; National Research Council of Italy, Institute of Science,
Technology and Sustainability for Ceramics (CNR-ISSMC former CNR-ISTEC), Via
Granarolo 64, 48018 Faenza, Italy.; Univ. Grenoble-Alpes, CEA, CNRS, IRIG, SyMMES,
CIBEST, 38000 Grenoble, France.; Research Centre POLARIS, Department of Earth and
Environmental Sciences, University of Milano-Bicocca, 20126 Milan, Italy.;
Department of Earth and Environmental Sciences, University of Milano-Bicocca,
Piazza della Scienza 1, 20126 Milan, Italy.
PY - 2023
ST - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
T2 - Toxics
TI - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
VL - 11
Y2 - 2 y3 - 20
ID - 9905
ER -
TY - JOUR
AB - Zinc nanostructures (ZnONSs) have attracted much attention due to their
morphological, physicochemical, and electrical properties, which were entailed for
various biomedical applications such as cancer and diabetes treatment, anti-
inflammatory activity, drug delivery. ZnONS play an important role in inducing
cellular apoptosis, triggering excess reactive oxygen species (ROS) production, and
releasing zinc ions due to their inherent nature and specific shape. Therefore,
several new synthetic organometallic method has been developed to prepare ZnO
crystalline nanostructures with controlled size and shape. Zinc oxide
nanostructures' crystal size and shape can be controlled by simply changing the
physical synthesis condition such as microwave irradiation time, reaction
temperature, and TEA concentration at reflux. Physicochemical properties which are
determined by the shape and size of ZnO nanostructures, directly affect their
biological applications. These nanostructures can decompose the cell membrane and
accumulate in the cytoplasm, which leads to apoptosis or cell death. In this study,
we reviewed the various synthesis methods which affect the nano shapes of zinc
particles, and physicochemical properties of zinc nanostructures that determined
the shape of zinc nanomaterials. Also, we mentioned some macromolecules that
controlled their physicochemical properties in a green and biological approaches.
In addition, we present the recent progress of ZnONSs in the biomedical fields,
which will help centralize biomedical fields and assist their future research
development.
AN - rayyan-553781026
AU - Mousavi, S. M.
AU - Behbudi, G.
AU - Gholami, A.
AU - Hashemi, S. A.
AU - Nejad, Z. M.
AU - Bahrani, S.
AU - Chiang, W. H.
AU - Wei, L. C.
AU - Omidifar, N.
DO - 10.1186/s40824-022-00252-y
IS - 1
KW - Zinc
PY - 2022
SN - 1226-4601 2055-7124
ST - Shape-controlled synthesis of zinc nanostructures mediating macromolecules
for biomedical applications
T2 - BIOMATERIALS RESEARCH
TI - Shape-controlled synthesis of zinc nanostructures mediating macromolecules
for biomedical applications
VL - 26
Y2 - 2 y3 - 2
ID - 9013
ER -
TY - JOUR
AB - Levamisole is a veterinary anti-helminthic used to treat several autoimmune
conditions but also commonly utilized as an additive in cocaine distribution.
Toxicity resulting in agranulocytosis and cutaneous necrosis in association with
cocaine use is an infrequently described phenomenon of an emerging problem.
Although levamisole is found extensively in the cocaine supply of the United
States, relatively few cases of necrotic skin lesions associated with intranasal
use have been reported. The skin necrosis secondary to levamisole toxicity is
characterized by variable findings on biopsy, ranging from leukocytoclastic
vasculitis to occlusive vasculopathy. The following case describes a 54-year-old
male who developed fever, agranulocytosis, p-ANCA autoantibodies and extensive skin
necrosis following heavy intranasal cocaine use. Necrosis of greater than 50% of
the patient's total body surface area resulted and was followed by thorough wound
debridement. Copyright © 2011 Journal of Drugs in Dermatology.
AN - rayyan-553781028
AU - Mouzakis, J.
AU - Somboonwit, C.
AU - Lakshmi, S.
AU - Rumbak, M.
AU - Sinnott, J.
AU - Cherpelis, B.
AU - Keshishian, J.
IS - 10
KW - Agranulocytosis
Antibodies, Antineutrophil Cytoplasmic
Cocaine
Cocaine-Related Disorders
Drug Contamination
Fever
Humans
Levamisole
Male
Middle Aged
Necrosis
Neutropenia
Skin
United States
bacitracin plus polymyxin B
beta glucan
cardiolipin antibody
cefepime
cocaine
doxycycline
fibrinogen
fluconazole
immunoglobulin M
levamisole
methylprednisolone
neutrophil cytoplasmic antibody
phospholipid
sulfadiazine silver
sulfamethoxazole
trimethoprim
adult
agranulocytosis
arm
article
back
blood culture
body surface
cannabis smoking
case report
cheek
chill
debridement
ear
erythema
erythrocyte sedimentation rate
face
fever
human
leg swelling
leukocyte count
lip
lymphadenopathy
malaise
male
mononuclear cell
neutropenia
neutrophil
neutrophil count
night sweat
nose
partial thromboplastin time
prothrombin time
skin biopsy
skin necrosis
thrombocyte count
trunk
urinalysis
vein thrombosis
PY - 2011
SP - 1204-1207
ST - Levamisole induced necrosis of the skin and neutropenia following intranasal
cocaine use: A newly recognized syndrome
T2 - Journal of Drugs in Dermatology
TI - Levamisole induced necrosis of the skin and neutropenia following intranasal
cocaine use: A newly recognized syndrome
80054718590&partnerID=40&md5=e7f1c6e7322c9a33cb4970a2f15c04ab
VL - 10
ID - 9014
ER -
TY - JOUR
AB - INTRODUCTION: Honey and chitosan have shown antimicrobial and wound healing
effects. As a biocompatible and biodegradable biomaterial, chitosan has shown
antimicrobial capabilities. OBJECTIVE: In this study, the effects of the
incorporation of high molecular weight chitosan hydrogel on antibacterial,
antifungal, and wound healing properties of honey were investigated. MATERIALS AND
METHODS: The minimum inhibitory concentration of chitosan and honey were examined
in pure and 3:1, 1:1, and 1:3 (v/v) compound ratios for Staphylococcus aureus,
Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In
addition, the inflammatory, granulation and fibrotic tissue formation,
reepithelialization indices, and wound shrinkage effects of each treatment were
evaluated and compared with saline and silver sulfadiazine. RESULTS: Chitosan/honey
1:3 was found to be effective against all 5 aforementioned germs. Honey,
chitosan/honey 1:1, and chitosan/honey 1:3 showed faster wound healing and
shrinkage effects. CONCLUSIONS: Incorporation of chitosan hydrogel into honey can
substantially enhance its antimicrobial and wound healing effects. Chitosan-
hydrogel/honey (1:3) is an optimal wound dressing formulation with ample
antimicrobial and healing properties.
AN - rayyan-553782405
AU - Movaffagh, J.
AU - Fazly Bazzaz, B. S.
AU - Yazdi, A. T.
AU - Sajadi-Tabassi, A.
AU - Azizzadeh, M.
AU - Najafi, E.
AU - Amiri, N.
AU - Taghanaki, H. B.
AU - Ebrahimzadeh, M. H.
AU - Moradi, A.
IS - 9
J2 - Wounds
KW - Animals
Anti-Infective Agents/*pharmacology
Bandages
Candida albicans/drug effects/isolation & purification
Chitosan/*pharmacology
*Disease Models, Animal
Escherichia coli/drug effects/isolation & purification
Honey
Hydrogels/*pharmacology
Pseudomonas aeruginosa/drug effects/isolation & purification
Rats
Staphylococcus aureus/drug effects/isolation & purification
Wounds and Injuries/*drug therapy/microbiology
Wound Healing
LA - eng
N1 - Pharmacological Research Center of Medicinal Plants, Mashhad University of
Medical Sciences, Mashhad, Iran.; Pharmacological Research Center of Medicinal
Plants, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of
Pathology, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.;
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical
Sciences, Mashhad, Iran.; Department of Clinical Sciences, Faculty of Veterinary
Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.; Pharmacological Research
Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.;
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical
Sciences, Mashhad, Iran.; Department of Chinese and Complementary Medicine, Faculty
of Persian and Complementary Medicine, Mashhad University of Medical Sciences,
Mashhad, Iran.; Orthopedic Research Center, Mashhad University of Medical Sciences,
Mashhad, Iran.; Orthopedic Research Center, Mashhad University of Medical Sciences,
Mashhad, Iran; Clinical Research Unit, Qaem Hospital, Faculty of Medicine, Mashhad
University of Medical Sciences.
PY - 2019
SP - 228-235
ST - Wound Healing and Antimicrobial Effects of Chitosan-hydrogel/Honey Compounds
in a Rat Full-thickness Wound Model
T2 - Wounds : a compendium of clinical research and practice
TI - Wound Healing and Antimicrobial Effects of Chitosan-hydrogel/Honey Compounds
in a Rat Full-thickness Wound Model
VL - 31
Y2 - 9
ID - 10312
ER -
TY - JOUR
AB - Pneumonia is a common but serious infectious disease, and is the sixth
leading cause for death. The foreign pathogens such as viruses, fungi, and bacteria
establish an inflammation response after interaction with lung, leading to the
filling of bronchioles and alveoli with fluids. Although the pharmacotherapies have
shown their great effectiveness to combat pathogens, advanced methods are under
developing to treat complicated cases such as virus-infection and lung inflammation
or acute lung injury (ALI). The inflammation modulation nanoparticles (NPs) can
effectively suppress immune cells and inhibit inflammatory molecules in the lung
site, and thereby alleviate pneumonia and ALI. In this review, the pathological
inflammatory microenvironments in pneumonia, which are instructive for the design
of biomaterials therapy, are summarized. The focus is then paid to the
inflammation-modulating NPs that modulate the inflammatory cells, cytokines and
chemokines, and microenvironments of pneumonia for better therapeutic effects. This
article is categorized under: Therapeutic Approaches and Drug Discovery >
Nanomedicine for Respiratory Disease
AN - rayyan-553781030
AU - Muhammad, W.
AU - Zhai, Z. H.
AU - Wang, S. Q.
AU - Gao, C. Y.
DO - 10.1002/wnan.1763
IS - 2
KW - Inflammation
Pneumonia
PY - 2022
SN - 1939-5116 1939-0041
ST - Inflammation-modulating nanoparticles for pneumonia therapy
T2 - WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
TI - Inflammation-modulating nanoparticles for pneumonia therapy
VL - 14
Y2 - 3
ID - 9015
ER -
TY - JOUR
AB - Graphene-based 2D nanomaterials exhibit unique physicochemical, electric, and
optical properties that facilitate applications in a wide range of fields including
material science, electronics, and biotechnology. Recent studies have shown that
graphene oxide (GO) and reduced graphene oxide (rGO) exhibit antimicrobial effects
on bacteria and viruses. While the bactericidal activity of graphene-based
nanomaterials is related to mechanical and oxidative damage to bacterial membranes,
their antiviral activity has been less explored. Currently available experimental
data are limited and suggest mechanical disruption of viral particles prior to
infection. In this study, the antiviral properties of reduced GO-based
nanocomposites decorated with Ag nanoparticles (rGO-Ag) are evidenced against human
immunodeficiency virus-1 pseudovirus used as an enveloped virus model. By combining
biochemical and original single virus imaging approaches, it is shown that rGO-Ag
induces peroxidation of pseudoviral lipid membrane and that consequent alteration
of membrane properties leads to a reduction in cell entry. In addition, rGO-Ag is
found to be efficiently internalized in the host cell leading to the elevated
expression of pro-inflammatory cytokines. Altogether, the presented results shed
new light on the mechanisms of rGO-Ag antiviral properties and confirm the high
potential of graphene derivatives as an antimicrobial material for biomedical
applications.
AN - rayyan-553781031
AU - Mukherjee, S.
AU - Bytesnikova, Z.
AU - Martin, S.
AU - Svec, P.
AU - Ridoskova, A.
AU - Pekarkova, J.
AU - Seguin, C.
AU - Weickert, J. L.
AU - Messaddeq, N.
AU - Mely, Y.
AU - Richtera, L.
AU - Anton, H.
AU - Adam, V.
DO - 10.1002/admi.202201996
IS - 6
PY - 2023
SN - 2196-7350
ST - Silver Nanoparticle-Decorated Reduced Graphene Oxide Nanomaterials Exert
Membrane Stress and Induce Immune Response to Inhibit the Early Phase of HIV-1
Infection
T2 - ADVANCED MATERIALS INTERFACES
TI - Silver Nanoparticle-Decorated Reduced Graphene Oxide Nanomaterials Exert
Membrane Stress and Induce Immune Response to Inhibit the Early Phase of HIV-1
Infection
VL - 10
Y2 - 2
ID - 9016
ER -
TY - JOUR
AB - The aim of this study was to synthesize L-cysteine stabilized silver
nanoparticles of 2 nm in size and determine their impact on cell viability. Silver
particles of this size range stabilized with L-cysteine woule be novel, as a
similar study by Mandal et al. on cysteine capped silver nanoparticles achieved
particles of only 4.4±0.3 nm. Currently, methods to synthesize and separate 2 nm
silver particles effectively are limited. Silver nanoparticles are an ideal
candidate for biological applications due to its natural anti-bacterial, anti-
inflammatory, and anti-platelet properties. For the 1st time, L-cysteine capped
silver nanoparticles 2±1 nm in diameter were synthesized and successfully separated
that also showed a non-significant impact on MCDK cell viability. Synthesis of the
silver nanoparticles was achieved via a reduction of AgNO 3 and NaBH 4. Capping was
achieved via a thiolate linkage between the amino acid and the silver particles
upon addition of L-cysteine to the colloid solution. Particles were stable for up
to 24 hours after which aggregates can be detected. Cell viability was determined
via a trypan blue stain for dead cells, and showed comparable levels of cell death
between control and nanoparticle treated cells. This study adds further evidence to
the role that particle size and surface charge play in overall cell viability from
silver nanoparticles. These characteristics can lead to the design of silver
nanoparticles that maintain clinical functionality while minimizing toxic effects.
© 2012 American Scientific Publishers All rights reserved.
AN - rayyan-553781034
AU - Mukherjee, S.
AU - Menegazzo, N.
AU - Booksh, K.
AU - Dhurjati, P.
AU - Smorodin, V.
AU - Nohe, A.
DO - 10.1166/asl.2012.2036
KW - Cysteine
Nanoparticles
Silver
Synthesis
Toxicity
Cell Survival
PY - 2012
SP - 26-33
ST - Synthesis of L-cysteine stabilized silver nanoparticles and their effects on
cell viability
T2 - Advanced Science Letters
TI - Synthesis of L-cysteine stabilized silver nanoparticles and their effects on
cell viability
84861500214&doi=10.1166%2fasl.2012.2036&partnerID=40&md5=137c2a464662b7d537d8e4ac21
a70713
VL - 6
ID - 9017
ER -
TY - JOUR
AB - The purpose of this study was to evaluate the biocompatibility, encrustation
and biodegradation properties of silver nitrate and ofloxacine blended
caprolactone-L-lactide copolymer coated self-reinforced poly-L-lactic acid (SR-
PLLA) urospirals in situ in the male rabbit urethra. SR-PLLA urospirals coated with
10% by weight silver nitrate or 5% by weight ofloxacine blended copolymer or pure
copolymer were inserted into the posterior urethra of 18 male rabbits. No
prophylactic antibiotics were given. The animals were sacrificed 1 or 6 months
after insertion. Urethral tissue reactions were histologically scored
semiquantitavely and the appearence of the stents assessed using scanning electron
microscopy. The biodegradation time of SR-PLLA stents was remarkably reduced by the
caprolactone coating. Silver nitrate and ofloxacine blended copolymer coated
urospirals caused less tissue reaction than urospirals with a pure copolymer
coating. Silver nitrate coating effectively prevented biofilm formation and stent
encrustation. Silver nitrate and ofloxacine blended copolymer coated SR-PLLA
urospirals had good biocompatibility properties in rabbit urethra. In particular,
coating with silver nitrate may provide possibilities of preventing bacterial
adhesion to bioabsorbable stents.
AN - rayyan-553781036
AU - Multanen, M.
AU - Tammela, T. L. J.
AU - Laurila, M.
AU - Seppälä, J.
AU - Välimaa, T.
AU - Törmälä, P.
AU - Talja, M.
DO - 10.1007/s00240-002-0252-7
IS - 4
KW - Bioabsorbable
Biocompatibility
Encrustation
Urethral stent
Animals
Anti-Infective Agents, Urinary
Biodegradation, Environmental
Biofilms
Coated Materials, Biocompatible
Crystallization
Male
Ofloxacin
Polyesters
Rabbits
Silver Nitrate
Stents
Urethra
ofloxacin
polylactic acid
silver nitrate
animal experiment
animal model
article
biocompatibility
biodegradability
biodegradation
controlled study
drug coating
drug effect
infection rate
inflammatory cell
nonhuman
priority journal
rabbit
stent
tissue reaction
RNA, Messenger
Polyglactin 910
Lactic Acid
Poly I-C
Poly A-U
PY - 2002
SP - 227-232
ST - Biocompatibility, encrustation and biodegradation of ofloxacine and silver
nitrate coated poly-L-lactic acid stents in rabbit urethra
T2 - Urological Research
TI - Biocompatibility, encrustation and biodegradation of ofloxacine and silver
nitrate coated poly-L-lactic acid stents in rabbit urethra
0036920067&doi=10.1007%2fs00240-002-0252-
7&partnerID=40&md5=bb962613329c8e61640998e1673fbdf4
VL - 30
ID - 9018
ER -
TY - JOUR
AB - Human biodistribution, bioprocessing and possible toxicity of nanoscale
silver receive increasing health assessment. We prospectively studied commercial
10- and 32-ppm nanoscale silver particle solutions in a single-blind, controlled,
cross-over, intent-to-treat, design. Healthy subjects (n = 60) underwent metabolic,
blood counts, urinalysis, sputum induction, and chest and abdomen magnetic
resonance imaging. Silver serum and urine content were determined. No clinically
important changes in metabolic, hematologic, or urinalysis measures were
identified. No morphological changes were detected in the lungs, heart or abdominal
organs. No significant changes were noted in pulmonary reactive oxygen species or
pro-inflammatory cytokine generation. In vivo oral exposure to these commercial
nanoscale silver particle solutions does not prompt clinically important changes in
human metabolic, hematologic, urine, physical findings or imaging morphology.
Further study of increasing time exposure and dosing of silver nanoparticulate
silver, and observation of additional organ systems are warranted to assert human
toxicity thresholds. From the Clinical Editor: In this study, the effects of
commercially available nanoparticles were studied in healthy volunteers, concluding
no detectable toxicity with the utilized comprehensive assays and tests. As the
authors rightfully state, further studies are definitely warranted. Studies like
this are much needed for the more widespread application of nanomedicine. © 2014
Elsevier Inc.
AN - rayyan-553781038
AU - Munger, M. A.
AU - Radwanski, P.
AU - Hadlock, G. C.
AU - Stoddard, G.
AU - Shaaban, A.
AU - Falconer, J.
AU - Grainger, D. W.
AU - Deering-Rice, C. E.
DO - 10.1016/j.nano.2013.06.010
IS - 1
KW - Biological activity nanoparticles
Nanotechnology
Nanotoxicology oral ingestion
Safety research
Adult
Aged
Blood Cell Count
Female
Heart
Humans
Lung
Magnetic Resonance Imaging
Male
Metal Nanoparticles
Middle Aged
Radiography, Thoracic
Silver
Sputum
Urinalysis
Bioactivity
Body fluids
Magnetic resonance imaging
Metabolism
Nanoparticles
Toxicity
silver nanoparticle
Health assessments
Healthy volunteers
Morphological changes
Nano particulates
Oral ingestion
abdomen
article
blood cell count
heart
human
in vivo study
intention to treat analysis
lung
nanotoxicology
normal human
organ systems
prospective study
sputum
urinalysis
Humanities
Humanism
PY - 2014
SP - 1-9
ST - In vivo human time-exposure study of orally dosed commercial silver
nanoparticles
T2 - Nanomedicine: Nanotechnology, Biology, and Medicine
TI - In vivo human time-exposure study of orally dosed commercial silver
nanoparticles
84890978504&doi=10.1016%2fj.nano.2013.06.010&partnerID=40&md5=8dc240f33a59229119add
46f7d9feff9
VL - 10
ID - 9019
ER -
TY - JOUR
AB - Calvaria from 6-day old mice labelled 4 days previously with 45CaCl2 were
divided into test and control halves and each half cultured separately in vitro.
Eluents from four endodontic materials, endomethasone, zinc oxide/eugenol, AH26 and
gutta-percha were added separately to the culture medium of each test half. After
24 and 48 h culturing periods, the 45CaCl2 in the media and calvaria was measured
by a standard liquid scintillation counting technique and a resorption ratio
between test and control halves was computed. This ratio, based on cell mediated
resorption, was an indication of toxicity of soluble components of each endodontic
material. In accordance with the literature, endomethasone was found, with our
method, to be the most toxic and gutta-percha the least toxic of the materials
tested. This shows that our model can be used to test the toxicity of other
biomaterials to bone cells in vitro.
AN - rayyan-553782239
AU - Murphy, W. M.
DO - 10.1016/0142-9612(85)90106-1
IS - 6
J2 - Biomaterials
Animals
Bismuth/pharmacology
Bone and Bones/*drug effects/physiology
Dexamethasone/pharmacology
Drug Combinations/pharmacology
*Epoxy Resins
Formaldehyde/pharmacology
Gutta-Percha/pharmacology
*Hydrocortisone
In Vitro Techniques
Methenamine/pharmacology
Mice
Root Canal Filling Materials/*pharmacology
Silver/pharmacology
Thymol/analogs & derivatives/pharmacology
Titanium/pharmacology
Zinc Oxide-Eugenol Cement/pharmacology
Bone and Bones
LA - eng
PY - 1985
SN - 0142-9612 (Print)
SP - 427-30
ST - An in vitro model for testing biocompatibility of endodontic materials to
bone
T2 - Biomaterials
TI - An in vitro model for testing biocompatibility of endodontic materials to
bone
VL - 6
Y2 - 11
ID - 10149
ER -
TY - JOUR
AB - The use of nanoparticles (NPs) for developing new therapeutic strategies in a
variety of diseases is gaining increasing attention. However, NPs could possess
undesired effects, including pro-inflammatory activities. Despite the fact that
several studies reported that NPs may induce or exacerbate eosinophilic
inflammation in vivo in rodents, the information regarding the direct interaction
between NPs and human eosinophils is lacking. In the present study, we test the
possibility that NPs could alter the capacity of human eosinophils to adhere onto a
cellular substratum. Using a panel of NPs, we found that several were able to
increase the adhesion of human eosinophil onto endothelial EA.hy926 cells. Among
them, TiO2 NPs were the most potent and we therefore pursue this study with these
NPs. TiO2 NPs were found to increase the adhesion of eosinophils in a concentration
dependent fashion. TiO2 NPs did not alter the cell surface expression of a panel of
cellular adhesion molecules, but CD29. Indeed, a weak to moderate, but significant,
decrease of CD29 was observed after 30 min but returned to normal levels after 90
min. TiO2 NPs were found to activate Akt, one important target of phosphoinositide
3-kinase (PI3K). However, despite the fact that cells were fully responsive to the
cytokine GM-CSF activating both Ala and Erk-1/2, TiO2 NPs did not activate Erk-1/2.
Using a pharmacological approach with the PI3K/Akt inhibitor, wortmannin, the
ability of TiO2 NPs to activate Akt was drastically inhibited and, further, their
capacity to increase adhesion of eosinophils was reversed. This study provides
insights into the effects of NPs on the biology of human eosinophils indicating
that as other agents, NPs, namely TiO2 NPs, can induce intracellular events
associated with a cellular function, adhesion.
AN - rayyan-553781071
AU - Murphy-Marion, M.
AU - Girard, D.
DO - 10.1016/j.imbio.2017.10.030
IS - 2
KW - Humanities
Humanism
Humans
Titanium
PY - 2018
SN - 0171-2985 1878-3279
SP - 162-170
ST - Titanium dioxide nanoparticles induce human eosinophil adhesion onto
endothelial EA.hy926 cells via activation of phosphoinositide 3-kinase/Akt cell
signalling pathway (Withdrawn Publication)
T2 - IMMUNOBIOLOGY
TI - Titanium dioxide nanoparticles induce human eosinophil adhesion onto
endothelial EA.hy926 cells via activation of phosphoinositide 3-kinase/Akt cell
signalling pathway (Withdrawn Publication)
VL - 223
Y2 - 2
ID - 9024
ER -
TY - JOUR
AB - The development and use of nanoparticles have alerted toxicologists and
regulators to issues of safety testing. By analogy with ambient air particles, it
can be expected that small doses are associated with a small increase in risk of
cardiovascular diseases, possibly through oxidative stress and inflammatory
pathways. We have assessed the effect of exposure to particulate matter on
progression of atherosclerosis and vasomotor function in humans, animals, and ex
vivo experimental systems. The type of particles that have been tested in these
systems encompass TiO2, carbon black, fullerene C60, single-walled carbon
nanotubes, ambient air particles, and diesel exhaust particles. Exposure to ambient
air particles is associated with accelerated progression of atherosclerosis and
vasomotor dysfunction in both healthy and susceptible animal models and humans at
risk of developing cardiovascular diseases. The vasomotor dysfunction includes
increased vasoconstriction as well as reduced endothelium-dependent vasodilatation;
endothelium-independent vasodilatation is often unaffected indicating mainly
endothelial dysfunction. Pulmonary exposure to TiO2, carbon black, and engineered
nanoparticles generate vasomotor dysfunction; the effect size is similar to that
generated by combustion-derived particles, although the effect could depend on the
exposure period and the administered dose, route, and mode. The relative risk
associated with exposure to nanoparticles may be small compared to some traditional
risk factors for cardiovascular diseases, but superimposed on these and possible
exposure to large parts of the population it is a significant public health
concern. Overall, assessment of vasomotor dysfunction and progression of
atherosclerosis are promising tools for understanding the effects of particulate
matter. © 2011 Informa Healthcare USA, Inc.
AN - rayyan-553781073
AU - Møller, P.
AU - Mikkelsen, L.
AU - Vesterdal, L. K.
AU - Folkmann, J. K.
AU - Forchhammer, L.
AU - Roursgaard, M.
AU - Danielsen, P. H.
AU - Loft, S.
DO - 10.3109/10408444.2010.533152
IS - 4
KW - Air pollution
atherosclerosis
endothelial dysfunction
nanoparticles
Air Pollutants
Animals
Atherosclerosis
Blood Vessels
Disease Progression
Humans
Nanoparticles
Particulate Matter
Vasoconstriction
Vasodilation
Vasomotor System
Animalia
1,1 diethyl 2 hydroxy 2 nitrosohydrazine
15 hydroxy 11alpha,9alpha epoxymethanoprosta 5,13 dienoic acid
3,3 bis(2 aminoethyl) 1 hydroxy 2 oxotriazene
adrenergic receptor blocking agent
apolipoprotein E
black carbon
calcimycin
cyclo(dextro tryptophyl dextro aspartylprolyl dextro valylleucyl)
fullerene
glyceryl trinitrate
hydrazine derivative
n (2,6 dimethylpiperidinocarbonyl) 4 methylleucyl dextro (1
methoxycarbonyltryptophanyl) dextro norleucine
nanoparticle
nitroprusside sodium
ozone
phenylephrine
silver nanoparticle
titanium dioxide
unclassified drug
vasodilator agent
verapamil
air pollution
ambient air
blood flow
blood vessel
cell proliferation
combustion
disease course
ecotoxicity
endothelium
ex vivo study
forearm blood flow
health hazard
human
inhalation
intracoronary infusion
lung
lung toxicity
nonhuman
particle size
particulate matter
public health
review
risk assessment
risk factor
shear stress
vasoconstriction
vasodilatation
vasomotor disorder
PY - 2011
SP - 339-368
ST - Hazard identification of particulate matter on vasomotor dysfunction and
progression of atherosclerosis
T2 - Critical Reviews in Toxicology
TI - Hazard identification of particulate matter on vasomotor dysfunction and
progression of atherosclerosis
79953134999&doi=10.3109%2f10408444.2010.533152&partnerID=40&md5=73077ada14eb5fc6a11
682bda22f124f
VL - 41
ID - 9026
ER -
TY - JOUR
AB - Silver is known to be a potent bactericidal agent. It has a proinflammatory
effect by inducing inflammatory cytokines and reactive oxygen species. Silver can
also have a direct cytotoxic effect on immune cells and can cause endothelial cell
injury.(1) At low levels, silver is thought to be nontoxic. It only deposits in the
skin and causes a silver-blue discoloration called argyria. Lately, there has been
an increase in the use of silver nanoparticles as antimicrobial agents or via
alternative medicine, which reignited interest in further studying silver toxicity.
In the CNS, silver can disrupt the blood-brain barrier and be toxic to neurons and
astrocytes.(1) There have been case reports of silver toxicity associated with
seizures, cortical basal degeneration, and psychosis.(2-4) On the other hand, the
effect of silver on the peripheral nervous system remains poorly explored. In this
report, we present a case of a peripheral neuropathy associated with argyria with
detailed clinical, laboratory, and histopathologic findings.
AN - rayyan-553781075
AU - Naddaf, E.
AU - Dyck, P. J.
AU - Jannetto, P. J.
AU - Murray, D. L.
AU - Dyck, P. J. B.
DO - 10.1212/WNL.0000000000007048
IS - 10
KW - Peripheral Nervous System Diseases
PY - 2019
SN - 0028-3878 1526-632X
SP - 481-483
ST - Peripheral neuropathy associated with silver toxicity
T2 - NEUROLOGY
TI - Peripheral neuropathy associated with silver toxicity
VL - 92
Y2 - 3 y3 - 5
ID - 9028
ER -
TY - JOUR
AB - Solutions containing Ag0 nanoclusters, Ag+1, and higher oxidation state
silver, generated from nanocrystalline silver dressings, were anti-inflammatory
against porcine skin inflammation. The dressings have clinically-demonstrated
broad-spectrum antimicrobial activity, suggesting application of nanosilver
solutions in treating pulmonary infection. Nanosilver solutions were tested for
antimicrobial efficacy; against HSV-1 and SARS-CoV-2; and nebulized in rats with
acute pneumonia. Patients with pneumonia (ventilated), fungal sinusitis, burns plus
COVID-19, and two non-hospitalized patients with COVID-19 received nebulized
nanosilver solution. Nanosilver solutions demonstrated pH-dependent antimicrobial
efficacy; reduced infection and inflammation without evidence of lung toxicity in
the rat model; and inactivated HSV-1 and SARS-CoV-2. Pneumonia patients had rapidly
reduced pulmonary symptoms, recovering pre-illness respiratory function. Fungal
sinusitis-related inflammation decreased immediately with infection clearance
within 21 days. Non-hospitalized patients with COVID-19 experienced rapid symptom
remission. Nanosilver solutions, due to anti-inflammatory, antiviral, and
antimicrobial activity, may be effective for treating respiratory inflammation and
infections caused by viruses and/or microbes. © 2023
AN - rayyan-553781076
AU - Nadworny, P. L.
AU - Hickerson, W. L.
AU - Holley-Harrison, H. D.
AU - Bloom, D. C.
AU - Grams, T. R.
AU - Edwards, T. G.
AU - Schultz, G. S.
AU - Burrell, R. E.
DO - 10.1016/j.nano.2023.102654
Antimicrobial
Antiviral
SARS-CoV-2
Animals
COVID-19
Inflammation
Pneumonia
Rats
Silver
Sinusitis
Swine
Fungi
Nanocrystals
Pathology
antiinfective agent
antivirus agent
colloidal silver
gelatinase A
gelatinase B
halothane
silver nanoparticle
silver nitrate
steroid
tobramycin
silver
Ag +
Anti-inflammatories
Antivirals
High oxidation state
Nano silver
Porcine skin
Silver dressing
adolescent
adult
allergic contact dermatitis
analysis of variance
animal experiment
animal model
animal tissue
apoptosis
Article
atomic absorption
bacterial pneumonia
bronchoscopy
burn
burn patient
cell infiltration
clinical evaluation
contact dermatitis
controlled study
coronavirus disease 2019
cream
dissolution
edema
epithelium cell
erythema
fungal sinusitis
guinea pig model
Human alphaherpesvirus 1
in vitro study
inflammatory cell
inoculation
interstitial pneumonia
multidrug resistant pneumonia
nebulization
neutrophil
nonhuman
off label drug use
oxygen consumption
pathogen clearance
pig
plaque forming unit
pneumonia
polymorphonuclear cell
rat
remission
thorax radiography
total body surface area
upper respiratory tract infection
virus inactivation
virus strain
animal
complication
inflammation
sinusitis
Coronavirus
Drug Resistance
Drug Resistance, Multiple
PY - 2023
ST - Treatment of infection and inflammation associated with COVID-19, multi-drug
resistant pneumonia and fungal sinusitis by nebulizing a nanosilver solution
TI - Treatment of infection and inflammation associated with COVID-19, multi-drug
resistant pneumonia and fungal sinusitis by nebulizing a nanosilver solution
85146655457&doi=10.1016%2fj.nano.2023.102654&partnerID=40&md5=1ace74f4a00f77748138d
1631b746aa3
VL - 48
ID - 9029
ER -
TY - JOUR
AB - The anti-inflammatory activity of nanocrystalline silver was examined using a
porcine model of contact dermatitis. Inflammation was induced with
dinitrochlorobenzene and then treated daily with nanocrystalline silver dressings,
0.5% silver nitrate, or saline. Erythema, edema, and histological data showed that
nanocrystalline silver-treated pigs had near-normal skin after 72 hours, while
other treatment groups remained inflamed. The decreased inflammation in the
nanocrystalline silver-treated group was associated with increased inflammatory
cell apoptosis, a decreased expression of proinflammatory cytokines, and decreased
gelatinase activity. Silver nitrate treatments induced apoptosis in all cell types,
including keratinocytes, resulting in delayed wound healing. These results
demonstrate that nanocrystalline silver had a direct anti-inflammatory effect in
the porcine contact dermatitis model that improved the overall outcome of the
healing process. These data offer support that a species of silver (e. g., Ag-0)
that is uniquely associated with nanocrystalline silver may be responsible for the
anti-inflammatory activity and improvement in healing. (C) 2008 Elsevier Inc. All
rights reserved.
AN - rayyan-553781079
AU - Wang, J. F.
AU - Tredget, E. E.
AU - Burrell, R. E.
DO - 10.1016/j.nano.2008.04.006
IS - 3
KW - Dermatitis, Contact
PY - 2008
SN - 1549-9634 1549-9642
SP - 241-251
ST - Anti-inflammatory activity of nanocrystalline silver in a porcine contact
dermatitis model
TI - Anti-inflammatory activity of nanocrystalline silver in a porcine contact
dermatitis model
VL - 4
Y2 - 9
ID - 9032
ER -
TY - JOUR
AB - Background. Nanocrystalline silver dressings have anti-inflammatory activity,
unlike solutions containing Ag+ only, which may be due to dissolution of multiple
silver species. These dressings can only be used to treat surfaces. Thus, silver-
containing solutions with nanocrystalline silver properties could be valuable for
treating hard-to-dress surfaces and inflammatory conditions of the lungs and
bowels. This study tested nanocrystalline silver-derived solutions for anti-
inflammatory activity. Methods. Inflammation was induced on porcine backs using
dinitrochlorobenzene. Negative and positive controls were treated with distilled
water. Experimental groups were treated with solutions generated by dissolving
nanocrystalline silver in distilled water adjusted to starting pHs of 4 (using
CO2), 5.6 (as is), 7, and 9 (using Ca(OH)2). Solution samples were analyzed for
total silver. Daily imaging, biopsying, erythema and oedema scoring, and treatments
were performed for three days. Biopsies were processed for histology,
immunohistochemistry (for IL-4, IL-8, IL-10, TNF-, EGF, KGF, KGF-2, and apoptotic
cells), and zymography (MMP-2 and -9). One-way ANOVAs with Tukey-Kramer post tests
were used for statistical analyses. Results. Animals treated with pH 7 and 9
solutions showed clear visual improvements. pH 9 solutions resulted in the most
significant reductions in erythema and oedema scores. pH 4 and 7 solutions also
reduced oedema scores. Histologically, all treatment groups demonstrated enhanced
re-epithelialisation, with decreased inflammation. At 24 h, pMMP-2 expression was
significantly lowered with pH 5.6 and 9 treatments, as was aMMP-2 expression with
pH 9 treatments. In general, treatment with silver-containing solutions resulted in
decreased TNF- and IL-8 expression, with increased IL-4, EGF, KGF, and KGF-2
expression. At 24 h, apoptotic cells were detected mostly in the dermis with pH 4
and 9 treatments, nowhere with pH 5.6, and in both the epidermis and dermis with pH
7. Solution anti-inflammatory activity did not correlate with total silver content,
as pH 4 solutions contained significantly more silver than all others. Conclusions.
Nanocrystalline silver-derived solutions appear to have anti-inflammatory/pro-
healing activity, particularly with a starting pH of 9. Solutions generated
differently may have varying concentrations of different silver species, only some
of which are anti-inflammatory. Nanocrystalline silver-derived solutions show
promise for a variety of anti-inflammatory treatment applications. © 2010 Nadworny
et al; licensee BioMed Central Ltd.
AN - rayyan-553781081
AU - Wang, J.
AU - Tredget, E. E.
AU - Burrell, R. E.
DO - 10.1186/1476-9255-7-13
KW - Animalia
Sus
Acticoat
dermatological agent
gelatinase A
gelatinase B
interleukin 10
interleukin 4
interleukin 8
nanocrystal
silver derivative
unclassified drug
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
contact dermatitis
controlled study
dermis
edema
epidermis
erythema
histopathology
imaging system
nonhuman
pH
protein expression
skin biopsy
swine
wound dressing
zymography
Dermatitis, Contact
PY - 2010
ST - Anti-inflammatory activity of nanocrystalline silver-derived solutions in
porcine contact dermatitis
T2 - Journal of Inflammation
TI - Anti-inflammatory activity of nanocrystalline silver-derived solutions in
porcine contact dermatitis
77649305432&doi=10.1186%2f1476-9255-7-
13&partnerID=40&md5=f13610b12b7591b0899f56740923dab6
VL - 7
ID - 9033
ER -
TY - JOUR
AB - In the present study, for the first time, biomimetization of hydroxyapatite
(HA) with Azadirachta indica (AI) was proposed and established its antioxidant,
antibacterial, and anti-inflammatory potential on lipopolysaccharide (LPS). The
ethanolic extract of AI was found rich with phenolics and flavonoids, and
determined their concentration as 8.98 +/- 1.41 mg gallic acid equivalents/g and
5.46 +/- 0.84 mg catechin equivalents/g, respectively. The HA was prepared by sol-
gel method from calcium nitrate tetrahydrate and orthophosphoric acid, and
successfully biomimetization was performed with ethanolic extract of AI. The FTIR
analysis settled that as-synthesized HA-AI composite was comprised of both HA and
AI. The XRD pattern and Zeta potential revealed that the HA-AI composite was
crystalline and negative in charge (-24.0 mV). The average-size distribution,
shape, and size of the HA-AI composite was determined as 238.90 d.nm, spherical,
and 117.90 nm from size distribution, SEM, and HR-TEM analysis, respectively. The
SEM-EDX concluded that the HA-AI composite was comprised of elements of HA as well
as AI. The HA-AI composite presented potential antioxidant activity and its EC50
values (dose required to inhibit about half of the radicals) for ABTS and DPPH
assays were determined as 115.72 +/- 2.33 and 128.51 +/- 1.04 mu g/ml,
respectively. The HA-AI composite showed potent antibacterial activity, and minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) towards
S. aureus (ATCC 700699) and E. coli (ATCC 10536) were correspondingly determined as
266.7 +/- 28.87 and 600.0 +/- 50.0 mu g/ml, and 400.0 +/- 86.6 and 816.7 +/- 76.38
mu g/ml. Most importantly, HA-AI composite presented the potential anti-
inflammatory response toward lipopolysaccharide (LPS) in RAW 264.7 cells. The dose
of 250 mu g/ml of HA-AI composite has shown optimum protection against LPS-induced
stress (1 mu g/ml) by scavenging oxidants and regulating mitochondrial membrane
potential (MMP), inflammatory and apoptotic factors. Thus, this study concluded
that the impartation of potential biofunctional features to HA from plant sources
through biomimetic approach is much beneficial and could find potential application
in dentistry and orthopedic.
AN - rayyan-553781082
AU - Nagaraj, A.
AU - Samiappan, S.
DO - 10.3389/fmicb.2019.01757
PY - 2019
SN - 1664-302X
ST - Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to
Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-
inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells
T2 - FRONTIERS IN MICROBIOLOGY
TI - Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to
Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-
inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells
VL - 10
Y2 - 8 y3 - 7
ID - 9034
ER -
TY - JOUR
AB - The current study investigates the hepatotoxic effects of two acute doses of
silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on African catfish (Clarias
garepinus) using biochemical, histopathological, and histochemical changes and the
determination of silver in liver tissue as biomarkers. AgNPs-induced impacts were
recorded in some of these characteristics based on their size (20 and 40 nm) and
their concentration (10 and 100 μg/L). Concentrations of liver enzymes (Aspartic
aminotransferase; AST, Alanine aminotransferase; ALT), alkaline phosphatase (ALP),
total lipids (Tl), Glucose (Glu) and Ag-concentration in liver tissue exhibited a
significant increase under stress in all exposed groups compared to the control
group. The total proteins (Tp), albumin (Al), and globulin (Gl) concentrations
exhibited significantly decrease in all treated groups compared to the control
group. At tissue and cell levels, histopathological changes were observed. These
changes include proliferation of hepatocytes, infiltrations of inflammatory cells,
pyknotic nuclei, cytoplasmic vaculation, melanomacrophages aggregation, dilation in
the blood vessel, hepatic necrosis, rupture of the wall of the central vein, and
apoptotic cells in the liver of AgNPs-exposed fish. As well as the depletion of
glycogen content in the liver (feeble magenta coloration) was observed. The size
and number of melanomacrophage centers (MMCs) in liver tissue showed highly
significant difference in all exposed groups compared to the control group.
Recovery period for 15 days led to improved most alterations in the biochemical,
histopathological, and histochemical parameters induced by AgNPs and AgNO3. In
conclusion, one can assume liver sensitivity of C. garepinus for AgNPs and the
recovery period is a must. © 2020
AN - rayyan-553781084
AU - Naguib, M.
AU - Mahmoud, U. M.
AU - Mekkawy, I. A.
AU - Sayed, A. E. D. H.
DO - 10.1016/j.toxrep.2020.01.002
KW - AgNPs
Biochemical
Clarias gariepinus
Hepatocyte
Histochemical
Histopathological
MMCs
albumin
globulin
silver nanoparticle
silver nitrate
animal tissue
Article
behavior assessment
cell aggregation
cell count
cell infiltration
cell proliferation
cell size
controlled study
drug analysis
drug determination
drug exposure
glucose level
histochemistry
histopathology
lipid blood level
liver injury
liver necrosis
liver tissue
liver toxicity
macrophage
melanomacrophage
morphology
mortality
nonhuman
nutritional deficiency
particle size
priority journal
vasodilatation
PY - 2020
SP - 133-141
ST - Hepatotoxic effects of silver nanoparticles on Clarias gariepinus;
Biochemical, histopathological, and histochemical studies
T2 - Toxicology Reports
TI - Hepatotoxic effects of silver nanoparticles on Clarias gariepinus;
Biochemical, histopathological, and histochemical studies
85077744804&doi=10.1016%2fj.toxrep.2020.01.002&partnerID=40&md5=2f7ad86d1bd17b4143c
41c49fe0a19c7
VL - 7
ID - 9036
ER -
TY - JOUR
AB - Plant intervened synthesis of nanoparticles has attracted an ample amount of
attention because this method is facile, cost-efficient, and eco-friendly. The
present study aims to synthesize AgNPs using Caesalpinia bonducella leaf extract by
employing a green chemistry approach and was characterized. UV-visible spectroscopy
revealed characteristic absorption maxima at 434 nm. FT-IR studies affirmed
presence of phytoconstituents that supports role of metabolites as a reducing and
capping agent. TEM and FESEM analysis reveal nanoparticles are spherical in shape
and EDS confirmed presence of elemental signature of silver at 3 KeV. XRD disclosed
crystalline nature of AgNPs with crystalline size of 15.17 nm and Zeta potential at
-30 mV indicates good stability. AgNPs showed good anti-inflammatory potential.
Anti-cancer activity of AgNPs revealed potential efficacy against MCF-7 cell lines
with 24 h IC50 value of 25 mu g/mL. Conferring results, present reports that the
biological synthesis method was found highly beneficial in fabrication
nanoparticles in medicinal applications.
AN - rayyan-553781085
AU - Naik, J. R.
AU - David, M.
DO - 10.1080/24701556.2021.2025093
PY - 2022
SN - 2470-1556 2470-1564
ST - Green synthesis of silver nanoparticles using Caesalpinia bonducella leaf
extract: characterization and evaluation of in vitro anti-inflammatory and anti-
cancer activities
TI - Green synthesis of silver nanoparticles using Caesalpinia bonducella leaf
extract: characterization and evaluation of in vitro anti-inflammatory and anti-
cancer activities
Y2 - 1 y3 - 3
ID - 9037
ER -
TY - JOUR
AB - Anti-inflammatory, analgesic, antipyretic, and gastrointestinal ulcerogenic
activities of 2-(8-methyl-10,11-dihydro-11-oxodibenz[b,f]oxepin-2-yl)propionic acid
(AD-1590), a new non-steroidal anti-inflammatory drug, were compared with
indomethacin (INN: indometacin) and other non-steroidal anti-inflammatory drugs
(NSAID) in experimental animals. 1. AD-1590 demonstrated potent inhibitory activity
on acute and subacute inflammation such as carrageenin hind paw edema (oral ED50 =
1.35 mg/kg), acetic acid-induced increased vascular permeability (0.205 mg/kg). UV-
erythema (0.295 mg/kg) and felt pellet-induced granuloma formation (1.7 mg/kg), and
its potency was on the whole 2 to 3 times that of indomethacin. 2. Oral analgesic
ED50-values of AD-1590 were 0.245, 8.32 and 13.9 mg/kg in the writhing tests, and
2.45 mg/kg in the silver nitrate-induced arthritic pain test. Analgesic potency of
AD-1590 was on the whole comparable to that of indomethacin. 3. Against the pyrexia
caused by two kinds of pyrogens (yeast and adjuvant). AD-1590 exerted a strong
antipyretic action at oral doses as low as 0.02 to 0.1 mg/kg, and its potency (ED50
= 0.0210 and 0.0406 mg/kg) was 8.7 to 11 times that of indomethacin. AD-1590
displayed the anti-pyretic activity at low doses which were widely different from
its anti-inflammatory and analgesic effective dose. The body temperature was not
affected by 20 mg/kg p.o. of AD-1590 in afebrile animals. AD-1590 was the strongest
antipyretic drug among 10 NSAID tested. 4. In rats, AD-1590 produced
gastrointestinal ulcer similar to indomethacin, and its gastric ulcerogenicity
(SUD50 = 13.8 mg/kg p.o.) was about one-half that of indomethacin. The activity of
AD-1590 in the fecal occult bleeding test in beagle dogs was weaker than that of
indomethacin. 5. The potency of AD-1590 (IC50 = 0.78 μmol/l) as a prostaglandin
synthetase inhibitor was about 2.7 times that of indomethacin in the in vitro test.
6. The safety index (SUD50/ED50) of AD-1590 was higher than that of indomethacin,
extremely high (the index = 657 and 340) in the antipyretic activity. Acute lethal
toxicity of AD-1590 (oral LD50 = 147 mg/kg in rats, 500 mg/kg in mice) was about
1/8 and 1/24 that of indomethacin. From these results, it was suggested that AD-
1590 had extraordinarily potent antipyretic activity, potent anti-inflammatory
activity superior to indomethacin, analgesic activity equivalent to indomethacin,
and a wide safety margin.
AN - rayyan-553781086
AU - Nakamura, H.
AU - Yokoyama, Y.
AU - Motoyoshi, S.
AU - Ishii, K.
AU - Imazu, C.
AU - Seto, Y.
AU - Kadokawa, T.
AU - Shimizu, M.
IS - 11
KW - Animals
Capillary Permeability
Dibenzoxepins
Erythema
Female
Fever
Guinea Pigs
Inflammation
Male
Mice
Pain
Peptic Ulcer
Pleurisy
Prostaglandins
Rats
acetic acid
adjuvant
alclofenac
arachidonic acid c 14
bermoprofen
carrageenan
clidanac
diclofenac
epirizole
flufenamic acid
ibuprofen
indometacin
kaolin
ketoprofen
mefenamic acid
naproxen
new drug
phenylbutazone
phenylquinone
radioisotope
silver nitrate
tiaramide
tolmetin
unclassified drug
analgesia
animal cell
animal experiment
animal model
arthritis
article
body temperature
digestive system
dog
dose response
drug comparison
drug mechanism
drug response
drug safety
drug screening
drug toxicity
edema
fever
guinea pig
intoxication
intraperitoneal drug administration
kidney
mouse
nonhuman
peripheral nervous system
prostaglandin synthetase inhibition
rat
subcutaneous drug administration
subcutaneous tissue
ulcer
yeast
Propionates
Diethylpropion
PY - 1983
SP - 1555-1569
ST - The pharmacological profile of 2-(8-methyl-10,11-dihydro-11-
oxodibenz[b,f]oxepin-2-yl)propionic acid (AD-1590), a new non-steroidal anti-
inflammatory agent with potent antipyretic activity
T2 - Arzneimittel-Forschung/Drug Research
TI - The pharmacological profile of 2-(8-methyl-10,11-dihydro-11-
oxodibenz[b,f]oxepin-2-yl)propionic acid (AD-1590), a new non-steroidal anti-
inflammatory agent with potent antipyretic activity
0021034919&partnerID=40&md5=b783d59f76704110baf4e9b0a23ec3be
VL - 33
ID - 9038
ER -
TY - JOUR
AB - Starting from the proligand 2-[(6-methylpyridin-2-yl)] imidazo[1,5-a]
pyridin-4-ylium hexafluorophosphate(1 center dot PF6), three new complexes, viz.
[Au(1)(2)] [PF6] (2), [1/2AuCl(2), 1/2AuCl(4)](-) [(1H)](+) (3), and [Au(1)Cl-3]
(4), have been synthesized and characterized employing different spectroscopic
methods. [Au(1)Cl-3] (4) has been synthesized by the disproportionation process.
During the transformation of 2 to 4, the annelated proligand stabilizes both Au(I)
and Au(III), and the isolation of the intermediate (3) confirms the conversion of
Au(I) - Au(III) through the disproportionation pathway. The solid state structures
of 2, 3 and 4 have been determined. Linear geometry was observed in 2, whereas
complex 4 adopted square-planar geometry. Gold complexes 2 and 4 have been
subjected to growth inhibitory studies. Complex 2 induced apoptosis in HepG2 cells
along with increased expression of proteins involved in the mitochondrial death
pathway, suggesting that apoptosis may occur via the mitochondrial death pathway.
AN - rayyan-553781090
AU - Nandy, A.
AU - Samanta, T.
AU - Mallick, S.
AU - Mitra, P.
AU - Seth, S. K.
AU - Das Saha, K.
AU - Al-Deyabe, S. S.
AU - Dinda, J.
DO - 10.1039/c5nj02979a
IS - 7
PY - 2016
SN - 1144-0546 1369-9261
SP - 6289-6298
ST - Synthesis of gold(III) <- gold(I)-NHC through disproportionation: the role of
gold(I)-NHC in the induction of apoptosis in HepG2 cells
TI - Synthesis of gold(III) <- gold(I)-NHC through disproportionation: the role of
gold(I)-NHC in the induction of apoptosis in HepG2 cells
VL - 40
Y2 - 7 y3 - 1
ID - 9042
ER -
TY - JOUR
AB - Wound healing, a complex biological process, has attained a lot of attention
as dermatologists are primarily interested in stimulated wound closure without
formation of scar or a faint scar. The recent upsurgence of nanotechnology has
provided novel therapeutic materials in the form of silver and gold nanoparticles
which accelerate the wound healing process. The effect of formulated nanoparticles
using Coleus forskohlii root extract (green synthesized) has been tried out for
ameliorating full thickness excision wounds in albino Wistar male rats. The
evaluation of in vivo activity of nanoparticles in wound healing was carried out on
open wounds made by excision on the dorsal sides of albino Wistar rats under
anesthesia, and the healing of the wounds was assessed. Histological aspects of the
healing process were studied by a HE (Hematoxylin and Eosin) staining method to
assess various degrees of re-epithelialization and the linear alignment of the
granulation tissue whereas Van Gieson's histochemical staining was performed to
observe collagen fibers. The healing action shown by the formulated nanoparticles
was remarkable during the early stages of wound healing, which resulted in the
substantial reduction of the whole healing period. Topical application of
formulated gold nanoparticles was found to be more effective in suppressing
inflammation and stimulating re-epithelialization compared to silver nanoparticles
during the healing process. The results throw light on the amelioration of excision
wounds using nanoparticles which could be a novel therapeutic way of improving
wound healing in clinical practice. The mechanism of advanced healing action of
both types of nanoparticles could be due to their antimicrobial, antioxidant and
anti-inflammatory properties. (C) 2016 Elsevier B.V. All rights reserved.
AN - rayyan-553781091
AU - Naraginti, S.
AU - Kumari, P. L.
AU - Das, R. K.
AU - Sivakumar, A.
AU - Patil, S. H.
AU - Andhalkar, V. V.
DO - 10.1016/j.msec.2016.01.069
KW - Rats
Rats, Wistar
PY - 2016
SN - 0928-4931 1873-0191
SP - 293-300
ST - Amelioration of excision wounds by topical application of green synthesized,
formulated silver and gold nanoparticles in albino Wistar rats
TI - Amelioration of excision wounds by topical application of green synthesized,
formulated silver and gold nanoparticles in albino Wistar rats
VL - 62
Y2 - 5 y3 - 1
ID - 9043
ER -
TY - JOUR
AB - Colorectal cancer (CRC) progression is a complex process, with an interplay
of multiple molecular signaling pathways. Cyclooxygenase-2 (COX-2) and NF-κB are
important hallmark proteins responsible for the transition from inflammation to
colon cancer. Due to the adverse effects of chemotherapeutic drugs, there is an
imperative need to develop new drugs, and recently, nanoparticles found to be
promising strategy in tumor detection, prevention and treatment of cancer.
Biosynthesis of silver nanoparticles (AgNPs) was achieved with the help of
Eucalyptus globulus L. leaf extract. Using a typical XRD pattern, NanoSight and TEM
technique, the size and shape of the biogenic AgNPs were determined as ~ 20 nm and
spherical. The cytotoxicity study exhibited a dose-dependent effect against HCT116
cells, with an inhibitory concentration (IC50) of 1.152 µg/ml. In addition, AgNPs
effectively inhibited the proliferation, colony formation, with increased ROS
production compared to untreated cells. Further, mechanistic analysis revealed that
AgNPs arrested the cell cycle, downregulated the expression of antiapoptotic,
inflammatory, stem cell markers, and upregulated the apoptotic genes in HCT116
cells. In conclusion, for the first time, we report the green synthesis of AgNPs
using E. globulus leaf extract that has potential anticancer activity with dual
inhibitory action on COX-2 and NF-κB expression. © 2021, The Author(s), under
exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
AN - rayyan-553781092
AU - Narasimha, V. R.
AU - Latha, T. S.
AU - Pallu, R.
AU - Panati, K.
AU - Narala, V. R.
DO - 10.1007/s10876-021-02143-z
IS - 5
KW - Anticancer activity
Colorectal cancer
Nanomedicine
Toxicity
Colonic Neoplasms
PY - 2022
SP - 2215-2231
ST - Anticancer Activities of Biogenic Silver Nanoparticles Targeting Apoptosis
and Inflammatory Pathways in Colon Cancer Cells
T2 - Journal of Cluster Science
TI - Anticancer Activities of Biogenic Silver Nanoparticles Targeting Apoptosis
and Inflammatory Pathways in Colon Cancer Cells
85112761273&doi=10.1007%2fs10876-021-02143-
z&partnerID=40&md5=37e710f06a4b8cdb2251181d51dee939
VL - 33
ID - 9044
ER -
TY - JOUR
AB - The green-mediated synthesis of copper nanoparticles is of great interest in
nanotechnology and is regarded as a low-cost and environmentally beneficial method.
Herein, Emilia sonchifolia leaf extract was employed as a reducing and capping
agent for the green production of copper nanoparticles. In this work, we focused on
the in vivo and in vitro biological studies of copper nanoparticles, which were
evaluated in zebrafish (Danio rerio) embryos. The biological effects from the in
vitro studies of the copper nanoparticles included cytotoxicity (in human cells)
and anti-diabetic, anti-inflammatory, and antibacterial activity. Furthermore, the
effectiveness of the greenly produced copper nanoparticles for photocatalysis was
also evaluated, and then SEM-EDX, FTIR, XRD, TGA and UV-vis spectroscopy were used
to characterise the copper nanoparticles. The results of the toxicity test on
zebrafish embryos demonstrated that the green-produced copper nanoparticles had a
significantly low harmful effect. According to the results, the copper
nanoparticles showed dose-dependent cytotoxicity against human keratinocytes
(HaCaT) and human breast cancer cells (MCF-7), which was higher than that of the
Emilia sonchifolia leaf extract. The green copper nanoparticles also demonstrated
more potent anti-inflammatory, antibacterial and anti-diabetic properties. In the
photocatalytic experiment, the produced copper nanoparticles successfully degraded
the organic methylene blue dye. Thus, it can be concluded that copper nanoparticles
can be employed for drug administration in both in vitro and in vivo settings in
biomedical applications. Additionally, as catalysts, these copper nanoparticles can
be employed for the removal of organic dyes.
AN - rayyan-553781093
AU - Narayanan, V. P. S.
AU - Kathirason, S. G.
AU - Elango, P.
AU - Subramanian, R.
AU - Sivagurusundar, R.
AU - Gurusamy, A.
DO - 10.1039/d3ra00454f
IS - 24
KW - Copper
PY - 2023
SN - 2046-2069
SP - 16724-16740
ST - Emilia sonchifolia leaf extract-mediated green synthesis, characterization,
in vitro biological activities, photocatalytic degradation and in vivo Danio rerio
embryo toxicity of copper nanoparticles
T2 - RSC ADVANCES
TI - Emilia sonchifolia leaf extract-mediated green synthesis, characterization,
in vitro biological activities, photocatalytic degradation and in vivo Danio rerio
embryo toxicity of copper nanoparticles
VL - 13
Y2 - 5 y3 - 30
ID - 9045
ER -
TY - JOUR
AB - Background: Fruits are an important dietary component, which supply vitamins,
minerals, as well as dietary fiber. In addition, they are rich sources of various
biological and pharmacologically active compounds. Among these, temperate fruits
are well studied for their pharmacological potentials, whereas tropical/subtropical
fruits are less explored for their health impacts. In India, most of the consumed
fruits are either tropical or subtropical. Objectives: This mini review aims to
provide a health impact of major tropical and sub-tropical fruits of India,
emphasizing their anticancer efficacy. In addition, the identified bioactive
components from these fruits exhibiting anticancer efficacy are also discussed
along with the patent literature published. Methods: The literature was collected
from various repositories, including NCBI, ScienceDirect, Eurekaselect, and Web of
Science; literature from predatory journals was omitted during the process. Patent
literature was collected from google patents and similar patent databases. Results:
Tropical fruits are rich sources of various nutrients and bioactive components
including polyphenols, flavonoids, anthocyanin, etc. By virtue of these
biomolecules, tropical fruits have been shown to interfere with various steps in
carcinogenesis, metastasis, and drug resistance. Their mode of action is either by
activation of apoptosis, regulation of cell cycle, inhibition of cell survival and
proliferation pathways, increased lipid trafficking or inhibiting inflammatory
pathways. Several molecules and combinations have been patented for their
anticancer and chemoprotective properties. Conclusion: Overall, the present
concludes that Indian tropical/ subtropical fruits are nutritionally and
pharmacologically active and may serve as a source of novel anticancer agents in
the future. © 2022 Bentham Science Publishers.
AN - rayyan-553781094
AU - Narayanankutty, A.
DO - 10.2174/1574892816666211130165200
IS - 2
bioactive compounds
cancer
degenerative diseases
Indian tropical fruits
Fruit
Humans
Nutritive Value
Patents as Topic
Plant Extracts
Polyphenols
acetogenin
Ananas comosus extract
Annona Squamosa extract
annonacin
anthocyanin
artocarpanone
artocarpin
Artocarpus altilis extract
Artocarpus heterophyllus extract
ascorbic acid
benzyl glucosinolate
beta carotene
bullatacin
caffeic acid
carbohydrate
Carica papaya extract
carotenoid
catechin
chlorogenic acid
coumaric acid
cyanidin
cyanomaclurin
cycloartocarpin
epicatechin
epigallocatechin
ferulic acid
gallic acid
Garcinia cambogia extract
gigantecin
guava extract
Hermes antigen
jacalin
malvidin chloride
Mangifera indica extract
mangiferin
mangostin
mangsterol
Manilkara zapota extract
moracin
Morinda citrifolia extract
motrilin
Musa acuminata cancer
Musa paradisiaca extract
myricetin
Nephelium litchi extract
nicotinamide
petunidin
plant extract
polyketide
polyphenol
pomegranate extract
protein
pterostilbene
pyrogallol
riboflavin
Semen coicis extract
silver nanoparticle
sinecatechins
Solanum nigrum extract
squamocin
syringic acid
Syzygium cumini extract
thiamine
unclassified drug
uvaricin
vanillic acid
vimentin
Zihiphud jujube extract
A-549 cell line
angiogenesis
Article
ascites hepatoma
ascites tumor
ash
breast cancer
breast cancer cell line
Caco-2 cell line
cancer growth
cancer stem cell
cancer therapy
carcinogenesis
cell cycle
cell cycle arrest
cell proliferation
cell survival
colon cancer
colon cell line
drug activity
drug mechanism
endometrium
endotoxemia
fruit
H22 cell line
HCT 116 cell line
Hep-G2 cell line
HT-29 cell line
human
India
leukemia cell
liver cell line
lung cell line
MCF-7 cell line
medicinal plant
melanoma cell
metastasis
nonhuman
nutritional value
oxidative stress
pharyngeal cell line
phytochemistry
prostate cell line
RNA sequence
skin cancer
stomach cancer
subtropical fruit
tropical fruit
patent
PY - 2022
SP - 124-135
ST - Pharmacological Potentials and Nutritional Values of Tropical and Subtropical
Fruits of India: Emphasis on their Anticancer Bioactive Components
T2 - Recent Patents on Anti-Cancer Drug Discovery
TI - Pharmacological Potentials and Nutritional Values of Tropical and Subtropical
Fruits of India: Emphasis on their Anticancer Bioactive Components
85129191905&doi=10.2174%2f1574892816666211130165200&partnerID=40&md5=ad71b8f8ef9827
b0810f720bbb1b0d96
VL - 17
ID - 9046
ER -
TY - JOUR
AB - Recently, green nanotechnology got great attention due to their reliable,
sustainable, and eco-friendly synthesis protocols. The green nanoparticles (GNPs)
are preferred over chemically synthesized nanoparticles owing to less destructive
effects associated with the synthesis procedures as well as therapeutic
involvement. In this review, we have discussed the applications of GNPs in
inflammation-mediated disorders, with special emphasis on cancer, initiated due to
oxidative stress and inflammatory cascade. Real-time mechanism based studies on
GNPs have suggested their anticancer effects through inducing apoptosis, inhibiting
angiogenesis, tissue invasion metastasis, reduced replicative capabilities in
addition to target specific different signaling molecules and cascades involved in
the development or progression of cancer. Moreover, the association of GNPs with
the inhibition or induction of autophagy for the management of cancer has also been
discussed. A large number of studies showed the GNPs have multifunctional
biomedical properties of theranostic prominence. Therefore, the development of GNPs
with naturally established systems could upsurge their definite applications as
biomedicines including target specific destruction of the cancerous cells. © 2022
Elsevier Ltd
AN - rayyan-553781095
AU - Naseer, F.
AU - Ahmed, M.
AU - Majid, A.
AU - Kamal, W.
AU - Phull, A. R.
DO - 10.1016/j.semcancer.2022.06.014
KW - Autophagy
Cancer
Green nanoparticles
Inflammation
Molecular mechanism
Nanomedicine
Apoptosis
Gold
Humans
Metal Nanoparticles
Neoplasms
ABC transporter
antiinfective agent
cyclin dependent kinase inhibitor
green nanoparticle
microRNA
nanoparticle
silver nanoparticle
tumor necrosis factor receptor associated factor 2
unclassified drug
vasculotropin
gold
metal nanoparticle
anemia
angiogenesis
antifungal activity
apoptosis
Aspergillus flavus
autophagy (cellular)
biomedicine
carcinogenesis
CD8+ T lymphocyte
cell cycle arrest
cell migration
cell proliferation
cell survival
constipation
DNA methylation
DNA replication
drug safety
Fusarium oxysporum
gene therapy
green chemistry
HT-1080 cell line
incubation time
MCF-7 cell line
metastasis
mitochondrial permeability
nanomedicine
nonhuman
oxidative stress
Parkinson disease
phagocytosis
Pi3K/Akt signaling
Review
Stenotrophomonas maltophilia
systematic review
tea
chemistry
human
neoplasm
Nanoparticles
PY - 2022
SP - 310-324
ST - Green nanoparticles as multifunctional nanomedicines: Insights into anti-
inflammatory effects, growth signaling and apoptosis mechanism in cancer
T2 - Seminars in Cancer Biology
TI - Green nanoparticles as multifunctional nanomedicines: Insights into anti-
inflammatory effects, growth signaling and apoptosis mechanism in cancer
85133851288&doi=10.1016%2fj.semcancer.2022.06.014&partnerID=40&md5=d747450dc00dafae
0132a076bed4cc5d
VL - 86
ID - 9047
ER -
TY - JOUR
AB - Oxidative stress is a destructive phenomenon that affects various cell
structures including membranes, proteins, lipoproteins, lipids, and DNA. Oxidative
stress and inflammation owing to lifestyle changes may lead to serious diseases
such as Cancers, Gout, and Arthritis etc. These disorders can be prevented using
different therapeutic strategies including nanomedicine. Biosynthesized gold
nanoparticles (GNPs) because of their anti-inflammatory and antioxidant
bioactivities can be key player in reversal of these ailments. This study was
carried out to evaluate the anti-inflammatory and antioxidant potential of bio
fabricated GNPs with Sarcococca saligna (S. saligna) extract on injured human
adipose-derived Mesenchymal stem cells (hADMSCs). GNPs were characterized by
ultraviolet-visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), x-
ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and energy
dispersive x-ray (EDS). Phytochemical screening of biosynthesized GNPs exhibited a
significant release of polyphenols, that is, total phenolic content (TPC) and total
flavonoid content (TFC). GNPs priming amended the in vitro injury caused by
Monosodium Iodoacetate (MIA) as exhibited by improved cell viability, wound closure
response and superoxide dismutase activity (SOD). The anti-inflammatory conduct
assessed through NF-kappa B pathway and other associated inflammatory markers
reported down-regulation of TNF-alpha (0.644 +/- 0.045), IL-1 beta (0.694 +/-
0.147) and IL-6 (0.622 +/- 0.112), apoptosis causing genes like Caspase-3 (0.734
+/- 0.13) and BAX (0.830 +/- 0.12), NF-kappa B pathway, p65 (0.672 +/- 0.084) and
p105 (0.539 +/- 0.083) associated genes. High SOD activity (95 +/- 5.25%) revealed
by treated hADMSCs with GNPs also supported the antioxidant role of GNPs in vitro
model. This study concludes that S. saligna bio fabricated GNPs priming may improve
the therapeutic potential of hADMSCs against chronic inflammatory problems by
regulating NF-kappa B pathway.
AN - rayyan-553781096
AU - Naseer, N.
AU - Mustafa, M. M.
AU - Latief, N.
AU - Fazal, N.
AU - Tariq, M.
AU - Afreen, A.
AU - Yaqub, F.
AU - Riazuddin, S.
DO - 10.1002/jbm.b.35303
KW - Humanities
Humanism
Humans
Oxidative Stress
PY - 2023
SN - 1552-4973 1552-4981
ST - Sarcococca saligna fabricated gold nanoparticles alleviated in vitro
oxidative stress and inflammation in human adipose-derived stem cells
TI - Sarcococca saligna fabricated gold nanoparticles alleviated in vitro
oxidative stress and inflammation in human adipose-derived stem cells
Y2 - 8 y3 - 10
ID - 9048
ER -
TY - JOUR
AB - Hepatitis C virus is a major causative agent of chronic liver disease. Viral
genotype, mutations, virus-host interaction, expression of viral proteins and host
immune-reaction are important factors in the pathogenesis of HCV infection. Precise
pathogenesis and perpetuation of hepatocellular injury in hepatitis C viral
infection remain unclear. Proposed mechanisms include direct viropathic effect, the
host immune response mediated through cytotoxic T lymphocytes, both viropathic and
cytopathic effects, and macrophages/monocytes. Apoptosis occurs both in acute or
chronic hepatitis and has been suggested to be mediated through Fas antigen. In HCV
infection, Fas expression is up-regulated in the liver cells in line with the
severity of liver inflammation. When HCV-specific T cells migrate into hepatocytes
and recognize the viral antigen via the T cell receptor, they become activated and
express Fas ligand that transduces the apoptotic death signal to Fas-bearing
hepatocytes resulting in their destruction. Thus, the Fas system plays an important
role in liver cell injury by HCV infection. Possible inducers of apoptosis in
hepatitis C include cytokines, especially tumor necrosis factor-alpha (TNF-alpha),
released by inflammatory cells, and acting through TNF and other cytokine
receptors.
AN - rayyan-553781097
AU - Nasir, A.
AU - Arora, H. S.
AU - Kaiser, H. E.
IS - 1
KW - Apoptosis
PY - 2000
SN - 0258-851X
SP - 297-300
ST - Apoptosis and pathogenesis of viral hepatitis C - An update
T2 - IN VIVO
TI - Apoptosis and pathogenesis of viral hepatitis C - An update
VL - 14
Y2 - 1
ID - 9049
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have become increasingly popular in the
biomedical field over the last few decades due to its proven antibacterial
property. Previous scientific studies have reported that one of the major organs
responsible for detoxification of AgNPs is the liver. The liver is also the primary
organ responsible for secretion of angiotensinogen (AGT), a key signaling molecule
involved in the renin-angiotensin system (RAS), which plays an important role in
maintaining cardiac output and vascular pressure. The aim of this study was to
assess any potential changes in the RAS-associated gene signaling, inflammatory
response, and hepatocellular toxicity resulting from AgNP exposure. To do this, 6-
week-old, male Wistar rats were exposed to a subacute inhalation exposure of AgNP
(200 ppb/days over 4 h/days exposure, for 5 d) and their livers were analyzed for
alterations in RAS components, inflammation, and oxidative stress. Real time qPCR
analysis showed that AgNP-exposure resulted in a significant increase in hepatic
AGT, angiotensin converting enzyme (ACE)-1, and ACE-2 mRNA expression. Expression
of inflammatory markers interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-
α were also upregulated with AgNP-exposure, compared to controls. Furthermore AgNP-
exposure mediated a significant increase in hepatic expression of catalase, and
superoxide dismutase, and oxidative stress, as assessed via 8-Oxo-2′-deoxyguanosine
staining. Increased oxidative stress was associated with increased
monocyte/macrophage-2 staining in the liver of AgNP-exposed rats. Such findings
indicate that subacute inhalation exposure to AgNPs mediate increased hepatic RAS
signaling, associated with inflammation, macrophage infiltration, and oxidative
stress. © 2021 Wiley Periodicals LLC.
AN - rayyan-553781098
AU - Nayek, S.
AU - Lund, A. K.
AU - Verbeck, G. F.
DO - 10.1002/tox.23412
IS - 3
KW - Animals
Inflammation
Inhalation Exposure
Male
Metal Nanoparticles
Oxidative Stress
Rats
Rats, Wistar
Silver
Cell death
Detoxification
Enzymes
Macrophages
Metal nanoparticles
Oxidative stress
Pathology
Signaling
8 hydroxydeoxyguanosine
angiotensin converting enzyme 1
angiotensin converting enzyme 2
catalase
cytochrome P450 1A1
dipeptidyl carboxypeptidase
interleukin 1beta
interleukin 6
messenger RNA
silver nanoparticle
unclassified drug
metal nanoparticle
silver
Biomedical fields
Hepatocellular
Hepatocellular toxicity
Inhalation exposure
Renin-angiotensin system
Scientific studies
Signaling molecules
Wistar rat
detoxification
induced response
nanoparticle
oxidative stress
pollution exposure
rodent
animal experiment
animal model
animal tissue
Article
cell infiltration
controlled study
DNA damage
gene expression
immune response
inflammation
liver fibrosis
liver toxicity
macrophage
male
monocyte
nonhuman
rat
receptor upregulation
renin angiotensin aldosterone system
signal transduction
adverse event
animal
exposure
Toxicity
PY - 2022
SP - 457-467
ST - Inhalation exposure to silver nanoparticles induces hepatic inflammation and
oxidative stress, associated with altered renin-angiotensin system signaling, in
Wistar rats
T2 - Environmental Toxicology
TI - Inhalation exposure to silver nanoparticles induces hepatic inflammation and
oxidative stress, associated with altered renin-angiotensin system signaling, in
Wistar rats
85119180312&doi=10.1002%2ftox.23412&partnerID=40&md5=c7e2789f1055d72c5d78c85d370944
21
VL - 37
ID - 9050
ER -
TY - JOUR
AB - Currently, metal nanostructures are widely used in medical, microbiological,
and veterinary practice. Silver nanoparticles are especially promising as
antimicrobial agents, becauseno published data regarding antimicrobial resistance
are available. Whiledeveloping preparations based on metal nanoparticles, an
important remainingissue is the choice of a stabilizer, introduction of which
during the synthesis ensures the preservation of structures at the nanoscale range,
and, consequently, relevant main characteristics, including biocidal properties.
The object of the study was to investigate silver nanoparticle aqueous dispersions
stabilized by natural and synthetic polymeric compounds. Routine strains of Gram-
positive and Gram-negative bacteria were used as experimental models: S. aureus 209
P, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 3177 (O-form), Klebsiella
pneumoniae ATCC 31488, obtained from the Scientific Centre for Expert Evaluation of
Medicinal Products. The antimicrobial activity of diverse variants of silver
nanoparticle aqueous dispersions was assessed by serial dilution platingon dense
nutrient medium. In this work, we examined no effect of silver nanoparticles
without stabilizers, because their absence led to rapid agglomeration of
nanostructures and loss of nanoscale characteristics. The highest sensitivity of
Gram-positive and Gram-negative bacteria was foundto the action of ansilver
nanoparticle aqueous dispersions stabilized by polyazolidinammoniumand modified
with iodine hydrate ions. Drug working concentrations ranging from 0.5 to 3% had a
bactericidal effect against pathogens of purulent-inflammatory diseases, and the
minimum working concentration of 0.125% led to decreased colony-forming units by
20-57% for diverse bacterial strains. Silver nanoparticles stabilized with sodium
dodecyl sulfate showed high efficiency against the studied test strainsprobably due
to the high toxicity of the stabilizer used as was previously established during a
comprehensive safety assessment using biotest objects and cell cultures. In this
regard, its use as a component of antimicrobial preparations is not preferred. The
results of the studies showed that among the variants of silver nanoparticle
aqueous dispersions, preparations stabilized with polyvinyl alcohol and
polyazolidinammonium modified with iodine hydrate ions are the most promising for
use in biomedical practice, because they demonstrate a high level of antibacterial
activity against both Gram-positive and Gram-negative bacteria as causative agents
of purulent-inflammatory diseases and a low toxicity level. This allows us to
recommend them as safe and effective antimicrobial components indisinfectants, as
well as antiseptic preparations for prevention and treatment of skin and soft
tissue infectious diseases. © 2022 Saint Petersburg Pasteur Institute. All rights
reserved.
AN - rayyan-553781099
AU - Nechaeva, O. V.
AU - Shulgina, T. A.
AU - Zubova, K. V.
AU - Glinskaya, E. V.
AU - Bespalova, N. V.
AU - Darin, N. I.
AU - Tichomirova, E. I.
AU - Afinogenova, A. G.
DO - 10.15789/2220-7619-AAO-1937
IS - 4
KW - antibiotic resistance
aqueous dispersions
causative agents of purulent-inflammatory diseases
polyazolidinammonium
polymers
ammonium derivative
iodine
nanomaterial
polymer
polyvinyl alcohol
pyrrole derivative
silver nanoparticle
aqueous solution
Article
bacterial strain
bacterium culture
dilution
Escherichia coli
hydration
infectious agent
inflammatory disease
nonhuman
nutrient
Proteus mirabilis
risk assessment
skin infection
soft tissue
soft tissue infection
PY - 2022
SP - 755-764
ST - ANTIMICROBIAL ACTIVITY of AQUEOUS DISPERSIONS of SILVER NANOPARTICLES against
PATHOGENS of PURULENT-INFLAMMATORY DISEASES
T2 - Russian Journal of Infection and Immunity
TI - ANTIMICROBIAL ACTIVITY of AQUEOUS DISPERSIONS of SILVER NANOPARTICLES against
PATHOGENS of PURULENT-INFLAMMATORY DISEASES
85140017238&doi=10.15789%2f2220-7619-AAO-
1937&partnerID=40&md5=0a3cbb3b8505355cc1e4c0a3a06dfb47
VL - 12
ID - 9051
ER -
TY - JOUR
AB - The biogenic manufacture of nanoparticles utilising endophytic fungus is an
eco-friendly, cost-effective, and secure alternative to constructing chemical
methods. The prime focus of the study was to fabricate ZnONPs using the biomass
filtrate of endophytic Xylaria arbuscula isolated from Blumea axillaris Linn. and
to evaluate their biological properties. The characterisation of the biosynthesized
ZnO-NPs was done utilising both spectroscopic and microscopic methods. The
bioinspired NPs showed a surface plasmon peak at 370 nm; SEM and TEM micrographs
illustrated the hexagonal organisation; XRD spectra proved the crystalline phase as
hexagonal wurtzite; EDX analysis confirmed the presence of zinc and oxygen atoms;
and the zeta potential analysis proved the stability of ZnONPs. In addition, they
also demonstrated significant concentration-dependent inhibition of antimicrobial,
antioxidant, anti-inflammatory, and antidiabetic potential in comparison with the
reference drugs. In vitro cytotoxicity and wound healing potential of ZnONPs were
examined in L929 cell lines, illustrating that they accelerated the wound healing
process by roughly 95.37 & PLUSMN; 1.12% after a 24-h exposure to ZnONPs. The
photocatalytic activity of the ZnONPs was examined by degrading the methylene blue
dye under solar irradiation. In conclusion, our outcomes showed that
mycosynthesized ZnONPs possessed potent bioactivity and could be an excellent
choice for biomedical applications.
AN - rayyan-553781100
AU - Nehru, L.
AU - Kandasamy, G. D.
AU - Sekar, V.
AU - Alshehri, M. A.
AU - Panneerselvam, C.
AU - Alasmari, A.
AU - Kathirvel, P.
DO - 10.1080/21691401.2023.2232654
IS - 1
PY - 2023
SN - 2169-1401 2169-141X
SP - 318-333
ST - Green synthesis of ZnO-NPs using endophytic fungal extract of Xylaria
arbuscula from Blumea axillaris and its biological applications
T2 - ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
TI - Green synthesis of ZnO-NPs using endophytic fungal extract of Xylaria
arbuscula from Blumea axillaris and its biological applications
VL - 51
Y2 - 12 y3 - 31
ID - 9052
ER -
TY - JOUR
AB - The objective of this study is to provide a novel synthetic approach for the
manufacture of wound-healing materials using covalently cross-linked alginate
fibers loaded with silver nanoparticles. Alginate fibers are prepared by wet-
spinning in a CaCl2 precipitation bath. Using this same approach, calcium cross-
links in alginate fibers are replaced by chemical cross-links that involve hydroxyl
groups for subsequent cross-linking by glutaraldehyde. The cross-linked fibers
become highly swollen in aqueous solution due to the presence of carboxyl
functional groups, and retain their mechanical stability in physiological fluids
owing to the stabilized network of covalent bonds. Alginate fibers can then be
loaded with silver ions via the ion-exchange reaction. Silver ions are reduced to
yield 11 nm silver nanoparticles incorporated in the polymer gel. This method
provides a convenient platform to incorporate silver nanoparticles into alginate
fibers in controlled concentrations while retaining the mechanical and swelling
properties of the alginate fibers. Our study suggests that the silver nanoparticles
loaded fibers may be easily applied in a wound healing paradigm and promote the
repair process though the promotion of fibroblast migration to the wound area,
reduction of the inflammatory phase, and the increased epidermal thickness in the
repaired wound area, thereby improving the overall quality and speed of healing. ©
2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AN - rayyan-553781101
AU - Neibert, K.
AU - Gopishetty, V.
AU - Grigoryev, A.
AU - Tokarev, I.
AU - Al-Hajaj, N.
AU - Vorstenbosch, J.
AU - Philip, A.
AU - Minko, S.
AU - Maysinger, D.
DO - 10.1002/adhm.201200075
IS - 5
KW - Absorbable Implants
Animals
Drug Carriers
Hydrogels
Metal Nanoparticles
Mice
Mice, Nude
Silver
Skin
Treatment Outcome
Wound Healing
Wounds, Penetrating
alginic acid
calcium ion
glutaraldehyde
silver nanoparticle
animal cell
animal experiment
animal model
aqueous solution
article
biodegradability
cell migration
cell viability
controlled study
covalent bond
cross linking
cytotoxicity test
drug effect
drug formulation
drug mechanism
drug stability
fibroblast migration
hydrogel dressing
in vitro study
in vivo study
ion exchange
mouse
nonhuman
particle size
pH
precipitation
priority journal
signal transduction
wound healing
Hydrogel
PY - 2012
SP - 621-630
ST - Wound-healing with mechanically robust and biodegradable hydrogel fibers
loaded with silver nanoparticles
TI - Wound-healing with mechanically robust and biodegradable hydrogel fibers
loaded with silver nanoparticles
84879608534&doi=10.1002%2fadhm.201200075&partnerID=40&md5=cc3a3c88837110415bfda82a1
d06f2eb
VL - 1
ID - 9053
ER -
TY - JOUR
AB - PP2A, a trimeric Serine/Threonine Protein Phosphatase 2A highly expressed in
brain, is a master regulator of cellular functions. Reduction in PP2A activity has
been linked to progression of microglial mediated neuroinflammatory diseases.
Inflammatory conditions are characterized by increased population of CD86(+ve) M1
cells and a therapeutic strategy to polarize microglial cells towards CD206(+ve) M2
cells is the need of hour. In this paper we analyzed A: whether the level of PP2A
is altered in CD86(+ve) cells, B: whether FTY720, a known modulator of PP2A, is
able to restore the level of PP2A in inflamed CD86(+ve) cells. Results revealed
that PP2A activity was significantly diminished in inflamed cells but the
surprising observation was the cell viability of only 35.99% upon FTY720 treatment
in inflamed cells lacking basal PP2A activity. A sharp increase at mRNA level of
CD95 and ASK-1 indicated that apoptosis occurred in these cells through CD95/ASK-
1/JNK pathway. Importantly, flow cytometric analysis revealed apoptosis of not only
CD86(+ve) cells but also CD206(+ve) cells. Previous studies have reported that
FTY720 polarizes microglial cells towards M2 states; however apoptosis of M2 cells
was not studied. As western blot analysis revealed that FTY720 failed to completely
restore PP2A, another PP2A modulator, Memantine, was used for co-treatment. Upon
co-treatment, the level of PP2A was completely restored and also viability of
microglial cells was significantly improved with a significant reduction in
apoptosis of M2 cells. These findings suggest that co-treatment strategy may prove
beneficial to balance M1/M2 microglial population, thereby improving neuronal
functions.
AN - rayyan-553781102
AU - Nematullah, M.
AU - Hoda, M. N.
AU - Nimker, S.
AU - Khan, F.
DO - 10.1016/j.taap.2020.115294
PY - 2020
SN - 0041-008X 1096-0333
ST - Restoration of PP2A levels in inflamed microglial cells: Important for
neuroprotective M2 microglial viability
TI - Restoration of PP2A levels in inflamed microglial cells: Important for
neuroprotective M2 microglial viability
VL - 409
Y2 - 12 y3 - 15
ID - 9054
ER -
TY - JOUR
AB - Wound dressings with silver have been shown to be cytotoxic in vitro.
However, the extrapolation of this cytotoxicity to clinical settings is unclear. We
applied dressings with various forms of silver on porcine skin ex vivo and
investigated silver penetration and DNA damage. We assessed antimicrobial efficacy,
cytotoxicity to skin cells, and immune response induced by the dressings. All
dressings elevated the DNA damage marker gamma -H(2)AX and the expression of
stress-related genes in explanted skin relative to control. This corresponded with
the amount of silver in the skin. The dressings reduced viability, induced
oxidative stress and DNA damage in skin cells, and induced the production of pro-
inflammatory IL-6 by monocytes. The oxidative burst and viability of activated
neutrophils decreased. The amount of silver released into the culture medium varied
among the dressings and correlated with in vitro toxicity. However, antimicrobial
efficiencies did not correlate strongly with the amount of silver released from the
dressings. Antimicrobial efficiency and toxicity are driven by the form of silver
and the construction of dressings and not only by the silver concentration. The
damaging effects of silver dressings in ex vivo skin highlight the importance of
thorough in vivo investigation of silver dressing toxicity.
AN - rayyan-553781105
AU - Nesporova, K.
AU - Pavlik, V.
AU - Safrankova, B.
AU - Vagnerova, H.
AU - Odraska, P.
AU - Zidek, O.
AU - Cisarova, N.
AU - Skoroplyas, S.
AU - Kubala, L.
AU - Velebny, V.
DO - 10.1038/s41598-020-72249-3
IS - 1
PY - 2020
SN - 2045-2322
ST - Effects of wound dressings containing silver on skin and immune cells
TI - Effects of wound dressings containing silver on skin and immune cells
VL - 10
Y2 - 9 y3 - 16
ID - 9057
ER -
TY - JOUR
AB - Wound dressings with silver have been shown to be cytotoxic in vitro.
However, the extrapolation of this cytotoxicity to clinical settings is unclear. We
applied dressings with various forms of silver on porcine skin ex vivo and
investigated silver penetration and DNA damage. We assessed antimicrobial efficacy,
cytotoxicity to skin cells, and immune response induced by the dressings. All
dressings elevated the DNA damage marker γ-H(2)AX and the expression of stress-
related genes in explanted skin relative to control. This corresponded with the
amount of silver in the skin. The dressings reduced viability, induced oxidative
stress and DNA damage in skin cells, and induced the production of pro-inflammatory
IL-6 by monocytes. The oxidative burst and viability of activated neutrophils
decreased. The amount of silver released into the culture medium varied among the
dressings and correlated with in vitro toxicity. However, antimicrobial
efficiencies did not correlate strongly with the amount of silver released from the
dressings. Antimicrobial efficiency and toxicity are driven by the form of silver
and the construction of dressings and not only by the silver concentration. The
damaging effects of silver dressings in ex vivo skin highlight the importance of
thorough in vivo investigation of silver dressing toxicity.
AN - rayyan-553781823
AU - Nešporová, K.
AU - Pavlík, V.
AU - Šafránková, B.
AU - Vágnerová, H.
AU - Odráška, P.
AU - Žídek, O.
AU - Císařová, N.
AU - Skoroplyas, S.
AU - Kubala, L.
AU - Velebný, V.
DO - 10.1038/s41598-020-72249-3
IS - 1
J2 - Sci Rep
KW - Animals
Bandages/*adverse effects
Cell Line
*DNA Damage
Humans
Silver/*toxicity
Skin/chemistry/*cytology/drug effects
Swine
Tissue Culture Techniques
Wound Infection
LA - eng
N1 - Contipro a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic.
kristina.nesporova@contipro.com.; Contipro a.s., Dolni Dobrouc 401, 56102, Dolni
Dobrouc, Czech Republic.; Third Faculty of Medicine, Charles University, Prague,
Czech Republic.; Contipro a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech
Republic.; Contipro a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic.;
Contipro a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic.; Contipro
a.s., Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic.; Contipro a.s.,
Dolni Dobrouc 401, 56102, Dolni Dobrouc, Czech Republic.; Faculty of Natural
Sciences, Charles University, Prague, Czech Republic.; Department of Biophysics of
Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno,
Czech Republic.; Department of Experimental Biology, Faculty of Science, Masaryk
University, Brno, Czech Republic.; Department of Biophysics of Immune System,
Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.;
Department of Experimental Biology, Faculty of Science, Masaryk University, Brno,
Czech Republic.; International Clinical Research Center, St. Anne's University
Hospital, Brno, Czech Republic.; Contipro a.s., Dolni Dobrouc 401, 56102, Dolni
Dobrouc, Czech Republic.
PY - 2020
SP - 15216
ST - Effects of wound dressings containing silver on skin and immune cells
TI - Effects of wound dressings containing silver on skin and immune cells
VL - 10
Y2 - 9 y3 - 16
ID - 9750
ER -
TY - JOUR
AB - Chronic wounds have emerged as a major cause of mortality, especially in
patients with diabetes and other pathologies. Statistics indicate that chronic
wounds affect around 6.5 million patients annually, with wound care and management
incurring huge economic costs. Growing incidence of chronic wounds and associated
pathologies along with the limitations of current therapies have established a
strong need for novel and innovative approaches to accelerate wound healing.
Conventionally, chronic wounds are addressed using various FDA-approved silver-
based formulations and other biomaterials. However, the toxicity associated with
these conventional approaches, along with the increased frequency of chronic wound
cases, makes the development of alternative therapies for effective wound healing
necessary. Recently, researchers have investigated the design and development of
nanoparticles, especially inorganic metal nanoparticles, as promising candidates
for addressing various pathological conditions, including wound healing. Several
research groups, including ours, have designed numerous metal nanoparticles
(including silver, gold, zinc oxide, cerium oxide, terbium hydroxide, silica,
titanium oxide, copper) and demonstrated their wound-healing properties using in
vitro and in vivo models. The rise of nanotechnology-based platforms in wound
healing is evidenced by the tremendous impact and number of publications observed
in recent years, which has emphasized the robust potential of inorganic
nanomedicine for addressing wounds. Therefore, the importance of these inorganic
nanomaterial-based interventions for wound-healing applications needs to be
emphasized to inform and encourage scientists and young researchers globally to
engage with this expanding area of biology and medicine. In this review article, we
mainly focus on highlighting the role of inorganic nanomaterials and nanomaterial-
based approaches for wound healing and tissue regeneration, along with their
mechanistic properties, clinical status, challenges, and future directions. © The
AN - rayyan-553781106
AU - Nethi, S. K.
AU - Das, S.
AU - Patra, C. R.
AU - Mukherjee, S.
DO - 10.1039/c9bm00423h
IS - 7
KW - Animals
Humans
Nanostructures
Nanotechnology
Signal Transduction
Wound Healing
Cerium oxide
Gold compounds
Pathology
Silica
Terbium compounds
Tissue regeneration
Titanium oxides
Zinc oxide
antiinfective agent
copper nanoparticle
glucocorticoid
gold nanoparticle
graphene oxide
nanomaterial
nanoparticle
nonmetallic inorganic nanoparticle
silica nanoparticle
silver nanoparticle
super paramagnetic iron oxide nanoparticle
terbium hydroxide nanoparticle
unclassified drug
biomaterial
Biology and medicine
Conventional approach
Design and Development
Innovative approaches
Inorganic nanomaterials
Mechanistic properties
Pathological conditions
angiogenesis
cell engineering
heart surgery
hemostasis
hemostasis phase
human
hyperbaric oxygen therapy
inflammatory phase
maturation phase
nanomedicine
nanotechnology
nerve regeneration
nonhuman
priority journal
proliferative phase
Review
tissue regeneration
vascular surgery
wound healing
animal
chemistry
drug effect
procedures
signal transduction
Metal nanoparticles
PY - 2019
SP - 2652-2674
ST - Recent advances in inorganic nanomaterials for wound-healing applications
T2 - Biomaterials Science
TI - Recent advances in inorganic nanomaterials for wound-healing applications
85067938096&doi=10.1039%2fc9bm00423h&partnerID=40&md5=e49f8d782dc1604dffe46cab695d1
040
VL - 7
ID - 9058
ER -
TY - JOUR
AN - rayyan-553782253
AU - Neuwirth, R. S.
AU - Richart, R. M.
AU - Taylor, H. C., Jr.
IS - 1
J2 - Obstet Gynecol
KW - *Acrylic Resins
Animals
Female
Fibroblasts
Granuloma/chemically induced
Haplorhini
Methods
Oviducts/*drug effects
Pregnancy
*Silver Nitrate
*Sterilization, Reproductive
*Zinc
LA - eng
PY - 1971
SN - 0029-7844 (Print)
SP - 51-4
ST - Chemical induction of tubal blockade in the monkey
T2 - Obstetrics and gynecology
TI - Chemical induction of tubal blockade in the monkey
VL - 38
Y2 - 7
ID - 10163
ER -
TY - JOUR
AB - Cerium oxide nanoparticle (CeO-NP) was synthesized using Origanum majorana L.
leaf extract and characterized using particle size analyzer, transmission electron
microscopy (TEM), field emission scanning electron microscopy (FESEM), X-ray
diffraction (XRD) and Fourier transform infrared (FTIR). The antioxidant properties
and cytotoxic effects of CeO-NP in human breast carcinoma cells (MDA-MB-231 cell
line) and human umbilical vein endothelial cells (HUVEC) as normal cells were
evaluated. To determine the probable molecular mechanism of action of CeO-NP on
cellular redox and anti-inflammatory potential, the expressions of antioxidant-
related genes catalase (CAT), superoxide dismutase (SOD) in HUVEC cell line were
also analyzed. The results indicated that spherically shaped nanoparticles with a
size of 10-70 nm bound to functional phenolic and flavonoids from O. majorana L.
leaf extract. The green synthesized CeO-NP showed antioxidant activity by free
radical scavenging activity against DPPH and ABTS free radicals. The antioxidant
activity was significantly (p < 0.001) lower than that of Butylated hydroxyanisole
(BHA) as a reference antioxidant. The obtained results elucidated that CeO-NP
possessed cytotoxicity. The cytotoxic effects of CeO-NP were higher against MDA-MB-
231 cancer cells compared to HUVEC normal cells. In addition, this NP was capable
to enhance the expression of CAT and SOD as main antioxidant-related genes.
Consequently, the higher cytotoxic effects of CeO-NP against breast cancer compared
to normal cells indicated the potential use of this NP as anti-cancer agent.
However, more research on its cytotoxicity against other cancer cells and
mechanisms in which this NP exert its anti-cancer properties should be performed.
AN - rayyan-553781108
AU - Nezhad, S. A.
AU - Es-haghi, A.
AU - Tabrizi, M. H.
DO - 10.1002/aoc.5314
IS - 2
PY - 2020
SN - 0268-2605 1099-0739
ST - Green synthesis of cerium oxide nanoparticle using Origanum majorana L. leaf
extract, its characterization and biological activities
T2 - APPLIED ORGANOMETALLIC CHEMISTRY
TI - Green synthesis of cerium oxide nanoparticle using Origanum majorana L. leaf
extract, its characterization and biological activities
VL - 34
Y2 - 2
ID - 9059
ER -
TY - JOUR
AB - Since about 70% of commercial biopharmaceutical products have been produced
in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for
biopharmaceuticals production. Meanwhile, sialic acid terminals were reported to
affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity
efficacy of IgG antibodies. Taking these findings together, we aimed to establish
CHO cell lines that highly produce sialic acid terminals by overexpressing two N-
acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-
acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate
reductase/methotrexate gene amplification method. Indeed, the number of total
sialic acid terminal glycan structures increased tremendously, by 12-fold compared
to the wild type in total protein extracts. With the methotrexate supplementation,
a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated
structures of glycoforms in total proteins. Interestingly, immunoglobulin G, used
as the model protein in CHOmt17-100, showed about 53% sialylated structures in its
glycoforms. These resultant sialylated glycans exhibited more than approximately
14.5 times increase as compared to that of the wild type. Moreover, the resultant
glycan structures mostly had N-acetylneuraminic acid terminals, while N-
glycolylneuraminic acid terminal composition remained less than 5% as compared to
the wild type. Engineered antibodies derived from CHO cell lines that produce high
levels of sialic acid will contribute to the examination of glycoforms’ efficacy
and usefulness toward bio-better products. © 2020, Springer Nature B.V.
AN - rayyan-553781112
AU - Nguyen, T. S.
AU - Misaki, R.
AU - Ohashi, T.
AU - Fujiyama, K.
DO - 10.1007/s10616-020-00381-z
IS - 3
KW - Antibody
Chinese hamster ovary (CHO) cell
Glycosylation
IgG
Sialyltransferase
UDP-GlcNAc 2-epimerase/ManNAc kinase
dihydrofolate reductase
glycan
immunoglobulin G
methotrexate
n acetylman nosamine kinase
n acetylneuraminic acid
phosphotransferase
recombinant antibody
sialic acid
sialyltransferase
unclassified drug
uridine diphosphate n acetylglucosamine 2 epimerase
animal cell
antibody dependent cellular cytotoxicity
Article
cell culture
cell density
cell viability
CHO cell line
comparative study
controlled study
female
gene amplification
internal ribosome entry site
methotrexate-resistant cell line
nonhuman
protein glycosylation
sialylation
silver staining
Cricetulus
Cricetinae
Ovary
PY - 2020
SP - 343-355
ST - Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary
cells
T2 - Cytotechnology
TI - Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary
cells
85080972492&doi=10.1007%2fs10616-020-00381-
z&partnerID=40&md5=044b5a2ad715309579f1b0ee77c9368e
VL - 72
ID - 9063
ER -
TY - JOUR
AB - 3D printing provides numerous opportunities for designing tissue engineering
constructs with intricate porosity, geometry and favourable mechanical properties
and has the potential to revolutionize medical treatments. However, an often-
encountered restriction is the selection of materials suitable for utilization in
3D printing, not all of which have appropriate biocompatibility properties. In this
work, fused deposition modeling was employed to fabricate 3D PCL constructs without
the use of any solvent. Plasma deposition was used to modify the surface of the
scaffolds, followed by immobilization of silver nanoparticles. The physico-chemical
and mechanical analyses demonstrated that the scaffolds retained their porosity and
mechanical integrity. The mechanical properties evaluated by the nanoindentation
technique demonstrated an increase in reduced modulus to 1.87 ± 0.012 GPa for PCL
scaffolds functionalized with silver nanoparticles for 24 hours. We also showed
complete prevention of colonization by medically relevant pathogens. The modified
scaffolds had good biocompatibility. The immune response studies in the culture of
macrophages confirmed a reduction in the level of expression of pro-inflammatory
cytokines which is a key requirement for successful wound healing. The in vivo
studies on Sprague Dawley rats indicated enhanced angiogenesis and the absence of
foreign body reaction for scaffolds functionalized with silver nanoparticles for 6
hours. The 3D printing approach presented in this study provides new sustainable
opportunities that can be adopted for designing biomaterial constructs with
enhanced biological properties. © 2021 The Royal Society of Chemistry.
AN - rayyan-553781116
AU - Ninan, N.
AU - Joseph, B.
AU - Visalakshan, R. M.
AU - Bright, R.
AU - Denoual, C.
AU - Zilm, P.
AU - Dalvi, Y. B.
AU - Priya, P. V.
AU - Mathew, A.
AU - Grohens, Y.
AU - Kalarikkal, N.
AU - Vasilev, K.
AU - Thomas, S.
DO - 10.1039/d1ma00444a
IS - 20
PY - 2021
SP - 6620-6630
ST - Plasma assisted design of biocompatible 3D printed PCL/silver nanoparticle
scaffolds: In vitro and in vivo analyses
T2 - Materials Advances
TI - Plasma assisted design of biocompatible 3D printed PCL/silver nanoparticle
scaffolds: In vitro and in vivo analyses
85117684440&doi=10.1039%2fd1ma00444a&partnerID=40&md5=d507c6d0b3d616e2c25ae85584919
55d
VL - 2
ID - 9067
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) are gaining a lot of attention in recent decades
from researchers due to their unique optoelectronic properties and their
significance in the field of biomedicine. Keeping this in view, our research work
was designed to investigate gold nanoparticles obtained by using a fungal
endophytic strain Chaetomium globosum, isolated from Vitex negundo which showed
significant activity on enzyme inhibition. In the present study, the fungal isolate
C. globosum was characterized using HPLC and LC-MS. A novel compound Catechin was
matched with standard Catechin. Further, the endophyte C. globosum extract was
utilized to synthesize gold nanoparticles (CgAuNPs) which was analysed by UV-
visible spectroscopy. The CgAuNPs exhibited wine red color and the absorption peak
appeared at 542 nm confirming the formation of the AuNPs. Further, Fourier
Transmission Infrared Spectroscopy (FTIR) was performed to confirm the various
functional groups present in mycosynthesized CgAuNPs. FTIR analysis demonstrated
the presence of amines, flavonoids, as well as the presence of amide I linkage
which possibly reduces Au+ to Au-0. The synthesized CgAuNPs exhibited potential
cytotoxicity against HeLa cells in a dose dependent manner. Further, CgAuNPs
demonstrated significant antiinflammatory activity. Overall, the present work
provides insights into the design of nano delivery and may be applied for clinical
studies in future.
AN - rayyan-553781117
AU - Ningaraju, S.
AU - Munawer, U.
AU - Raghavendra, V. B.
AU - Balaji, K. S.
AU - Melappa, G.
AU - Brindhadevi, K.
AU - Pugazhendhi, A.
DO - 10.1016/j.ab.2020.113970
KW - Chaetomium
PY - 2021
SN - 0003-2697 1096-0309
ST - Chaetomium globosum extract mediated gold nanoparticle synthesis and potent
anti-inflammatory activity
T2 - ANALYTICAL BIOCHEMISTRY
TI - Chaetomium globosum extract mediated gold nanoparticle synthesis and potent
anti-inflammatory activity
VL - 612
Y2 - 1 y3 - 1
ID - 9068
ER -
TY - JOUR
AB - SUMMARY: Skeletal muscle injury is an acute inflammatory condition caused by
an inflammatory response. To reduce inflammatory cell infiltration and relieve
skeletal muscle injury, efficient treatment is urgently needed. Nitric oxide is a
free radical molecule reported to have anti-inflammatory effects. In this study, we
showed that NO could inhibit the inflammatory response of C2C12 cells in vitro and
protect rat skeletal muscle injury from notexin in vivo. NO synthase inhibitor (L-
NG-Nitroarginine Methyl Este?L-NAME) and NO donor (sodium nitroprusside dehydrate ?
SNP) were used to explore the vital role of lipopolysaccharides (LPSs) in LPS-
stimulated C2C12 myoblasts.The expression of IL-18 and IL-1b was upregulated by L-
NAME and downregulated by SNP, as indicated by the ELISA results. NO can reduce
ASC, Caspase-1, and NLRP3 mRNA and protein levels. Furthermore, NO was detected in
the rat model. The results of immunohistochemical staining showed that the
production of DMD decreased. We conducted qRT-PCR and western blotting to detect
the expression of Jo-1, Mi-2, TLR2, and TLR4 on day 6 post injury following
treatment with L-NAME and SNP. The expression of Jo-1, Mi-2, TLR2, and TLR4 was
upregulated by L-NAME and significantly reversed by SNP. NO can alleviate C2C12
cell inflammatory responses and protect rat skeletal muscle injury from notexin.
RESUMEN: La lesión del músculo esquelético es una afección inflamatoria aguda
causada por una respuesta inflamatoria. Para reducir la infiltración de células
inflamatorias y aliviar la lesión del músculo esquelético es necesario un
tratamiento eficaz. El óxido nítrico es una molécula de radicales libres que tiene
efectos antiinflamatorios. En este estudio, demostramos que el ON podría inhibir la
respuesta inflamatoria de las células C2C12 in vitro y proteger la lesión del
músculo esquelético de rata de la notexina in vivo. El inhibidor de ON sintasa (L-
NG-nitroarginina metil este, L-NAME) y el donante de ON (nitroprusiato de sodio
deshidratado, SNP) se utilizaron para explorar el papel vital de los
lipopolisacáridos (LPS) en los mioblastos C2C12 estimulados por LPS. La expresión
de IL- 18 e IL-1b fue regulada positivamente por L-NAME y regulada negativamente
por SNP, como indican los resultados de ELISA. El ON puede reducir los niveles de
proteína y ARNm de ASC, Caspasa-1 y NLRP3. Además, se detectó ON en el modelo de
rata. Los resultados de la tinción inmunohistoquímica mostraron que disminuyó la
producción de DMD. Realizamos qRT-PCR y transferencia Western para detectar la
expresión de Jo-1, Mi-2, TLR2 y TLR4 el día 6 después de la lesión después del
tratamiento con L-NAME y SNP. La expresión de Jo-1, Mi-2, TLR2 y TLR4 fue regulada
positivamente por L- NAME y significativamente revertida por SNP. El ON puede
aliviar las respuestas inflamatorias de las células C2C12 en ratas, y proteger la
lesión del músculo esquelético de la notexina.
AN - rayyan-553781120
AU - Niu, Shu-liang
AU - Jin, Xiu-Fang
AU - Yang, Tao
AU - Chen, Zhi-Hui
AU - Wang, Guang-Dong
AU - Liang, Ying
AU - Yan, Jie
DO - 10.4067/S0717-95022022000100251
IS - 1
KW - C2C12
Inflamación
Inflamasoma
Inflammasome
Inflammation
Lesión muscular
Muscle injury
Nitric oxide
Óxido nítrico
LA - en
PY - 2022
SN - 0717-9367
SP - 251-260
ST - Effects of nitric oxide on C2C12 cell inflammatory responses and notexin-
induced muscle injury
TI - Effects of nitric oxide on C2C12 cell inflammatory responses and notexin-
induced muscle injury
VL - 40
Y2 - 2
ID - 9071
ER -
TY - JOUR
AB - Aim: The aim of the study is to synthesis Hybanthus enneaspermus mediated
silver nanoparticles and to evaluate the cytotoxic and antioxidant potential.
Introduction: Hybanthus enneaspermus (F. Muell) belongs to the family Violaceae.
The plant Hybanthus enneaspermus is reported to have antimicrobial, anticonvulsant,
nephroprotective, hepatoprotective, anti-inflammatory, aphrodisiac, aldose
reductase inhibitory and free radical scavenging activities. Ag NPs produce
reactive oxygen species and free radicals causing apoptosis (cell death) thereby
preventing their replication. Many researchers have correlated the plants
antioxidant potential with their phenolic constituents. Antioxidants have
resistance against oxidative stress by scavenging free radicals, thereby preventing
many diseases. Materials and Methods: Cytotoxic activity: Brine shrimp eggs were
obtained from the new aqua laboratory. Filtered artificial seawater was prepared,
then shrimp eggs were added into the dark side of the chamber while there was a
lamp above the other side to attract the hatched shrimp. Two days were allowed for
shrimp to mature, after two days the shrimp Larva was ready. 10 brine shrimps were
added accordingly to the silver nanoparticle. It added 5, 10, 15, 20 micrometer and
it was left for 24 hrs and observation was made. Antioxidant activity: 50%
methanol, DPPH solution and Hybanthus enneaspermus mediated silver nanoparticles
were added in 5 test tubes ranging from 10-50 microliters and kept in a dark place
for 10 minutes and the reading was recorded using photometry. Results and
Discussion: In the cytotoxic activity as the concentration of the silver
nanoparticle increases the percentage of lethality of brine shrimps also increases.
In the antioxidant activity as the concentrations of silver nanoparticles increases
the percentage of inhibition also increases. Conclusion: With the help of the study
we can conclude that silver nanoparticles of Hybanthus enneaspermus possess both
cytotoxic and antioxidant activity. © 2020 Society for Biology and Biotechnology.
All rights reserved.
AN - rayyan-553781121
AU - Nivethitha, S.
AU - Arivarasu, L.
IS - 31
KW - Antioxidant activity
BSLA
Cytotoxic activity
DPPH
Hybanthus enneaspermus
Silver nanoparticle
Antioxidants
PY - 2020
SP - 104-110
ST - Cytotoxic and antioxidant potential of hybanthus enneaspermus mediated silver
nanoparticle
T2 - Plant Cell Biotechnology and Molecular Biology
TI - Cytotoxic and antioxidant potential of hybanthus enneaspermus mediated silver
nanoparticle
85092937779&partnerID=40&md5=571fd96d16430eb730de86ea0d12867a
VL - 21
ID - 9072
ER -
TY - JOUR
AB - BACKGROUND: Particulate matter (PM) is one of the six criteria pollutant
classes for which National Ambient Air Quality Standards have been set by the
United States Environmental Protection Agency. Exposures to PM have been correlated
with increased cardio-pulmonary morbidity and mortality. Butadiene soot (BDS),
generated from the incomplete combustion of 1,3-butadiene (BD), is both a model PM
mixture and a real-life example of a petrochemical product of incomplete
combustion. There are numerous events, including wildfires, accidents at refineries
and tank car explosions that result in sub-acute exposure to high levels of
airborne particles, with the people exposed facing serious health problems. These
real-life events highlight the need to investigate the health effects induced by
short-term exposure to elevated levels of PM, as well as to assess whether, and if
so, how well these adverse effects are resolved over time. In the present study, we
investigated the extent of recovery of mouse lungs 10 days after inhalation
exposures to environmentally-relevant levels of BDS aerosols had ended. METHODS:
Female BALB/c mice exposed to either HEPA-filtered air or to BDS (5 mg/m(3) in HEPA
filtered air, 4 h/day, 21 consecutive days) were sacrificed immediately, or 10 days
after the final BDS exposure. Bronchoalveolar lavage fluid (BALF) was collected for
cytology and cytokine analysis. Lung proteins and RNA were extracted for protein
and gene expression analysis. Lung histopathology evaluation also was performed.
RESULTS: Sub-acute exposures of mice to hydrocarbon-rich ultrafine particles
induced: (1) BALF neutrophil elevation; (2) lung mucosal inflammation, and (3)
increased BALF IL-1β concentration; with all three outcomes returning to baseline
levels 10 days post-exposure. In contrast, (4) lung connective tissue inflammation
persisted 10 days post-exposure; (5) we detected time-dependent up-regulation of
biotransformation and oxidative stress genes, with incomplete return to baseline
levels; and (6) we observed persistent particle alveolar load following 10 days of
recovery. CONCLUSION: These data show that 10 days after a 21-day exposure to 5
mg/m(3) of BDS has ended, incomplete lung recovery promotes a pro-
biotransformation, pro-oxidant, and pro-inflammatory milieu, which may be a
starting point for potential long-term cardio-pulmonary effects.
AN - rayyan-553782417
AU - Noël, A.
AU - Xiao, R.
AU - Perveen, Z.
AU - Zaman, H. M.
AU - Rouse, R. L.
AU - Paulsen, D. B.
AU - Penn, A. L.
DO - 10.1186/s12989-016-0122-z
KW - Administration, Inhalation
Aerosols
Animals
Bronchoalveolar Lavage Fluid/chemistry
Butadienes/administration & dosage/*toxicity
Environmental Pollutants/administration & dosage/*toxicity
Female
Gene Regulatory Networks
Lung/*drug effects/metabolism/pathology
Mice, Inbred BALB C
Neutrophil Infiltration/drug effects
Particle Size
Pneumonia/*chemically induced/genetics/metabolism/pathology
Recovery of Function
Risk Assessment
Soot/administration & dosage/*toxicity
Time Factors
Mice
Lung
LA - eng
N1 - Department of Comparative Biomedical Sciences, School of Veterinary Medicine,
Louisiana State University, Skip Bertman Dr., Baton Rouge, LA, 70803, USA.;
Department of Anesthesiology, Columbia University Medical Center, New York, NY,
USA.; Department of Comparative Biomedical Sciences, School of Veterinary Medicine,
Louisiana State University, Skip Bertman Dr., Baton Rouge, LA, 70803, USA.;
Department of Comparative Biomedical Sciences, School of Veterinary Medicine,
Louisiana State University, Skip Bertman Dr., Baton Rouge, LA, 70803, USA.; United
States Food and Drug Administration, Silver Spring, MD, USA.; Department of
Pathobiological Sciences, School of Veterinary Medicine, Louisiana State
University, Baton Rouge, LA, 70803, USA.; Department of Comparative Biomedical
Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman
Dr., Baton Rouge, LA, 70803, USA. apenn1@lsu.edu.
PY - 2016
SP - 10
ST - Incomplete lung recovery following sub-acute inhalation of combustion-derived
ultrafine particles in mice
TI - Incomplete lung recovery following sub-acute inhalation of combustion-derived
ultrafine particles in mice
VL - 13
Y2 - 2 y3 - 24
ID - 10324
ER -
TY - JOUR
AB - In recent years, research on silver nanoparticles (AgNPs) has attracted
considerable interest among scientists because of, among other things, their
alternative application to well-known medical agents with antibacterial properties.
The size of the silver nanoparticles ranges from 1 to 100 nm. In this paper, we
review the progress of research on AgNPs with respect to the synthesis,
applications, and toxicological safety of AgNPs, and the issue of in vivo and in
vitro research on silver nanoparticles. AgNPs' synthesis methods include physical,
chemical, and biological routes, as well as "green synthesis". The content of this
article covers issues related to the disadvantages of physical and chemical
methods, which are expensive and can also have toxicity. This review pays special
attention to AgNP biosafety concerns, such as potential toxicity to cells, tissues,
and organs.
AN - rayyan-553781122
AU - Noga, M.
AU - Milan, J.
AU - Frydrych, A.
AU - Jurowski, K.
DO - 10.3390/ijms24065133
IS - 6
PY - 2023
SN - 1422-0067
ST - Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver
Nanoparticles (AgNPs)-Critical Review: State of the Art
TI - Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver
Nanoparticles (AgNPs)-Critical Review: State of the Art
VL - 24
Y2 - 3
ID - 9073
ER -
TY - JOUR
AB - Engineered nanomaterials are emerging functional materials with
technologically interesting properties and a wide range of promising applications,
such as drug delivery devices, medical imaging and diagnostics, and various other
industrial products. However, concerns have been expressed about the risks of such
materials and whether they can cause adverse effects. Studies of the potential
hazards of nanomaterials have been widely performed using cell models and a range
of in vitro approaches. In the present review, we provide a comprehensive and
critical literature overview on current in vitro toxicity test methods that have
been applied to determine the mechanisms underlying the cytotoxic effects induced
by the nanostructures. The small size, surface charge, hydrophobicity and high
adsorption capacity of nanomaterial allow for specific interactions within cell
membrane and subcellular organelles, which in turn could lead to cytotoxicity
through a range of different mechanisms. Finally, aggregating the given information
on the relationships of nanomaterial cytotoxic responses with an understanding of
its structure and physicochemical properties may promote the design of biologically
safe nanostructures.
AN - rayyan-553781123
AU - Nogueira, D. R.
AU - Mitjans, M.
AU - Rolim, C. M. B.
AU - Vinardell, M. P.
DO - 10.3390/nano4020454
IS - 2
PY - 2014
SN - 2079-4991
SP - 454-484
ST - Mechanisms Underlying Cytotoxicity Induced by Engineered Nanomaterials: A
Review of In Vitro Studies
T2 - NANOMATERIALS
TI - Mechanisms Underlying Cytotoxicity Induced by Engineered Nanomaterials: A
Review of In Vitro Studies
VL - 4
Y2 - 6
ID - 9074
ER -
TY - JOUR
AB - Due to rapidly increasing resistance development against conventional
antibiotics, finding novel approaches for the treatment of infections has emerged
as a key health issue. Antimicrobial peptides (AMPs) have attracted interest in
this context, and there is by now a considerable literature on the identification
such peptides, as well as on their optimization to reach potent antimicrobial and
anti-inflammatory effects at simultaneously low toxicity against human cells. In
comparison, delivery systems for antimicrobial peptides have attracted considerably
less interest. However, such delivery systems are likely to play a key role in the
development of potent and safe AMP based therapeutics, e.g., through reducing
chemical or biological degradation of AMPs either in the formulation or after
administration, by reducing adverse side-effects, by controlling AMP release rate,
by promoting biofilm penetration, or through achieving co-localization with
intracellular pathogens. Here, an overview is provided of the current understanding
of delivery systems for antimicrobial peptides, with special focus on AMP-carrier
interactions, as well as consequences of these interactions for antimicrobial and
related biological effects of AMP-containing formulations. (C) 2017 Elsevier B.V.
AN - rayyan-553781124
AU - Nordstrom, R.
AU - Malmsten, M.
DO - 10.1016/j.cis.2017.01.005
KW - C-Peptide
Peptide T
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Peptide PHI
Peptides
PY - 2017
SN - 0001-8686 1873-3727
SP - 17-34
ST - Delivery systems for antimicrobial peptides
T2 - ADVANCES IN COLLOID AND INTERFACE SCIENCE
TI - Delivery systems for antimicrobial peptides
VL - 242
Y2 - 4
ID - 9075
ER -
TY - JOUR
AB - With recent progress in the manufacture and applications of nickel oxide
nanoparticles (NiO NPs), concerns about their adverse effects are increasing.
Hesperidin (HSP) is a citrus flavonoid that has a potent anti-inflammatory,
antioxidant and free radical scavenging activities. This study aims to investigate
the protective effect of HSP against testicular and spermatological damages induced
by NiO NPs in male rats. Forty rats were randomly and equally divided into four
groups: control, NiO NPs, HSP and NiO NPs + HSP. NiO NPs (100 mg/kg) and/or HSP
(100 mg/kg) were given daily by gavage for 60 days. Exposure to NiO NPs induced
marked reproductive toxicity in male rats that was manifested by increased sperm
abnormalities and deterioration of sperm motility, count and viability. NiO NPs
also increased lipid peroxidation and negatively affected the cellular antioxidant
defense system in the testis of rats. The level of serum testosterone hormone was
increased in NiO NPs-exposed rats. qPCR showed a marked downregulation in
expression of steroidogenesis-related genes (CYP11A1, HSD3B and STAR) and a
significant upregulation in expression of apoptosis-related gene (caspase-9) in
testicular tissue of rats. Various pathological lesions and an increase in the
number of PCNA-positive immune-reactive cells were also noticed in the testis of
NiO NPs-exposed rats. Co-administration of HSP significantly ameliorated most of
the NiO NPs-induced testicular damages and improved male fertility in rats.
AN - rayyan-553781125
AU - Noshy, P. A.
AU - Khalaf, A. A. A.
AU - Ibrahim, M. A.
AU - Mekkawy, A. M.
AU - Abdelrahman, R. E.
AU - Farghali, A.
AU - Tammam, A. A.
AU - Zaki, A. R.
DO - 10.1016/j.tox.2022.153208
KW - Nickel
Rats
PY - 2022
SN - 0300-483X 1879-3185
ST - Alterations in reproductive parameters and steroid biosynthesis induced by
nickel oxide nanoparticles in male rats: The ameliorative effect of hesperidin
T2 - TOXICOLOGY
TI - Alterations in reproductive parameters and steroid biosynthesis induced by
nickel oxide nanoparticles in male rats: The ameliorative effect of hesperidin
VL - 473
Y2 - 5 y3 - 15
ID - 9076
ER -
TY - JOUR
AB - Studies have demonstrated that the prolonged use of corticoids can delay the
healing process, affecting re-epithelialization, neovascularization and collagen
synthesis. As the fins of teleost fish contain a large amount of collagen, the aim
of the present study was to investigate the effect of dexamethasone (anti-
inflammatory and glucocorticoid steroid widely used in the treatment of rheumatic
diseases) during the regeneration process in the caudal fin of specimens of carp
(Cyprinus carpio). For such, two glass aquaria were used – one for a group of fish
treated with dexamethasone (Henrifarma) in a 20 mg/L concentration and the other
for the control group. The caudal fins were amputated transversally and fish
remained in their respective aquaria until regeneration occurred. Samples of
regenerating fins were collected on days 1, 2, 4, 6, 8 and 10 after amputation. The
fins in the control group regenerated normally and grew within the expected in time
course. The fins in the group treated with dexamethasone were significantly smaller
in comparison to the control group at every evaluation time. Thus, it was possible
to verify that, at this concentration of dexamethasone, the regeneration of the
caudal fins was delayed, but not completely inhibited. The results show that the
caudal fin is a good model for histological studies on regeneration and the action
of drug toxicity, but it’s also of great importance the interaction with further
studies for a better knowledge and understanding of all the changes in all the
phases. Estudos mostram que corticóides usados por longos períodos podem atrasar o
processo de cicatrização, influenciando na reepitelização, na neovascularização e
na síntese do colágeno. Os constituintes das nadadeiras dos peixes teleósteos
contêm grande quantidade de colágeno e assim o objetivo do presente trabalho foi
estudar o efeito da dexametasona (um antiinflamatório e glicocorticóide esteróide
bastante utilizado no tratamento de doenças reumáticas) durante o processo
regenerativo das nadadeiras caudais das carpas (Cyprinus carpio). Para isso, foram
montados dois aquários de vidro, um para o grupo controle e outro para o grupo
tratado com a dexametasona (Henrifarma) na concentração de 20mg/L. Os peixes
distribuídos nesses aquários tiveram suas nadadeiras caudais amputadas
transversalmente e permaneceram nos respectivos aquários para que ocorresse a
regeneração. Foram feitas coletas das nadadeiras em regeneração em intervalos de 1,
2, 4, 6, 8 e 10 dias após a amputação. Foi observado que nos peixes do grupo
controle, as nadadeiras regeneraram normalmente e cresceram o esperado em cada
intervalo de tempo. No entanto, foi verificado que nos peixes do grupo tratado com
dexametasona, em cada intervalo analisado, as nadadeiras regeneradas dos peixes
expostos à droga eram menores que a medida das nadadeiras dos peixes do grupo
controle. Assim, foi possível verificar que, nessa concentração de dexametasona, a
regeneração das nadadeiras caudais foi mais lenta, mas não ocorreu a total inibição
da regeneração. Dessa forma, os resultados comprovam que a nadadeira caudal é um
bom modelo para estudos histológicos sobre a regeneração e a ação da toxicidade de
drogas, mas, também, é de grande importância a interação com estudos mais
aprofundados para se conhecer e compreender melhor todas as alterações em todas as
fases.
AN - rayyan-553781126
AU - Ochandio, B. S.
AU - Bechara, I. J.
AU - Parise-Maltempi, P. P.
DO - 10.1590/1519-6984.16813
IS - 2
KW - Caudal fin
Collagen
Colágeno
Dexametasona
Dexamethasone
Fish
Nadadeira caudal
Peixe
Regeneração tecidual
Tissue regeneration
Regeneration
LA - en
PY - 2015
SN - 1519-6984
SP - 442-450
ST - Dexamethasone action on caudal fin regeneration of carp Cyprinus carpio
(Linnaeus, 1758)
T2 - Braz. j. biol
TI - Dexamethasone action on caudal fin regeneration of carp Cyprinus carpio
(Linnaeus, 1758)
69842015000200027
VL - 75
Y2 - 5 y3 - 1
ID - 9077
ER -
TY - JOUR
AB - Peri-implantitis is an inflammatory disease with a relevant focus on the
long-term success of dental implants and implant-supported prostheses. The present
study focuses on the antibacterial effect of the silver nanoparticle and
investigated the suppression of dental plaque adhesion on implant abutment and/or
superstructure by micro-wave assistant nanosilver coating in vivo and in vitro.
Nanosilver coating on pure titanium was prepared by microwave-assisted synthesis,
and characterized by scanning electron microscopy and energy-dispersive X-ray
spectroscopy. In vitro studies were conducted to analyze biocompatibility using MTS
assay and fluorescence microscopy with human gingival fibroblasts to evaluate
antibacterial activity. During the in vivo study, nanosilver coating was applied to
the healing abutments, and the prevention of plaque accumulation on nanosilver
coating was confirmed by a split-mouth randomized clinical trial. The aggregation
of nano-sized particles was found on the titanium surface with an antibacterial
effect. The coating had no cytotoxic effect on human gingival fibroblasts. The
result of the clinical trial showed that the coating suppressed the dental plaque
adhesion on the healing abutments. Nanosilver coating is a promising material with
antibacterial properties and can be used for implant abutments and prostheses for
preventing peri-implantitis. © 2020 by the authors.
AN - rayyan-553781127
AU - Odatsu, T.
AU - Kuroshima, S.
AU - Sato, M.
AU - Takase, K.
AU - Valanezhad, A.
AU - Naito, M.
AU - Sawase, T.
IS - 6
KW - Antibacterial
Microwave synthesis
Nanosilver
Peri-implantitis
nanocoating
silver nanoparticle
titanium
adult
aged
Article
biocompatibility
cell adhesion
cell proliferation
clinical article
colony forming unit
controlled study
female
fibroblast
human
human cell
male
microwave assisted synthesis
microwave radiation
MTS assay
priority journal
radiological procedures
randomized controlled trial
tooth plaque
PY - 2020
SP - 1-11
ST - Antibacterial properties of nano-ag coating on healing abutment: An in vitro
and clinical study
T2 - Antibiotics
TI - Antibacterial properties of nano-ag coating on healing abutment: An in vitro
and clinical study
85090605696&doi=10.3390%2fantibiotics9060347&partnerID=40&md5=fcc39e19478a50aa6c62a
d6758c0c98d
VL - 9
ID - 9078
ER -
TY - JOUR
AB - Chronic inflammatory wounds pose therapeutic challenges in the biomedical
field. Polymeric nanofibrous matrices provide extracellular-matrix-like structures
to facilitate wound healing; however, wound infection and the subsequent
accumulation of reactive oxygen species (ROS) delay healing. Therefore, we herein
developed electrospun nanofibers (NFs), composed of chitosan-stabilized Prussian
blue (PBChi) nanoparticles (NPs) and poly(vinyl alcohol) (PVA), with ROS scavenging
activity to impart antioxidant and wound healing properties. The PBChi NPs were
prepared using chitosan with different molecular weights, and their weight ratio
with respect to PVA was optimized to yield PBChi-NP-coated PVA NFs with well-
defined NF structures. In situ and in vitro antioxidant activity assays showed that
the PBChi/PVA NFs could effectively remove ROS. Particularly, PBChi/PVA NFs with a
lower chitosan molecular weight exhibited greater antioxidant activity. The
hydroxyl radical scavenging activity of PBChi10k/PVA NFs was 60.4 %, approximately
two-fold higher than that of PBChi100k/PVA NFs. Further, at the concentration of 10
mu g/mL, they could significantly lower the in vitro ROS level by up to 50.7 %. The
NFs caused no significant reduction in cell viability, owing to the excellent
biocompatibility of PVA with PBChi NPs. Treatment using PBChi/PVA NFs led to faster
cell proliferation in in vitro scratch wounds, reducing their size from 202 to 162
mu m. The PBChi/PVA NFs possess notable antioxidant and cell proliferation
properties as ROS-scavenging wound dressings.
AN - rayyan-553781128
AU - Oh, H.
AU - Son, D.
AU - Lee, J. S.
AU - Kim, M.
AU - Sung, D. K. Y.
AU - Lee, H. K.
AU - Il Choi, W.
DO - 10.1016/j.ijbiomac.2022.08.033
KW - Wound Healing
Oxygenators
PY - 2022
SN - 0141-8130 1879-0003
SP - 835-843
ST - Reactive oxygen species scavenging nanofibers with chitosan-stabilized
Prussian blue nanoparticles for enhanced wound healing efficacy
TI - Reactive oxygen species scavenging nanofibers with chitosan-stabilized
Prussian blue nanoparticles for enhanced wound healing efficacy
VL - 219
Y2 - 10 y3 - 31
ID - 9079
ER -
TY - JOUR
AB - Despite the dangers associated with the increased use of codeine drugs,
limited researches have addressed the specific effects of emzolyn codeine on the
lung. The aim of this study was to assess the histological effects of emzolyn
codeine cough syrup on the lung of Wistar rats and its oxidative stress. Twenty one
(21) Wistar rats were divided into 3 groups labeled T1, T2 and T3. Group T1 served
as control and was given distilled water and diet for 42 days, group T2 was treated
with 0.1 mg/g bodyweight emzolyn codeine cough syrup for 21 days while group T3 was
treated with 0.1 mg/g bodyweight emzolyn codeine cough syrup for 42 days. At the
end of the duration, the wistar rats were sacrificed under anaesthesia and the
lungs were collected after dissection and transferred into 10% buffered formalin.
Sections of the lungs were obtained and processed for histological studies using
Hematoxylin and Eosin stain, Periodic acid Schiff's solution, Phosphotungstic acid
Haematoxylin stain and Methanamine Silver stains. Results from the study suggested
that acute and chronic exposure to emzolyn codeine cough syrup produced significant
(P<0.05) decrease in body weight, edematous aveolar space with marked type 11
pneumocyte,marked hypertrophy (H) of the septa and marked inflammatory cells. The
levels of total antioxidant status (TAS) was also determined using standard
spectrophotometric techniques. The mean MDA of the exposed groups were
significantly higher while the mean levels of SOD, GPx, CAT, and GSH were
significantly lower than the control group. In conclusion, this study confirmed the
risk of increased oxidative stress, pulmonary toxicity and decreased body weight
due to emzolyn codeine cough syrup administration. Thus, indiscriminately and
prolong use emzolyn codeine drug should be avoided and antioxidant supplements are
advised as a prophylactic supportive therapy for adequate measures in preventing
development of oxidative stress-associated complications among exposed individuals.
AN - rayyan-553781129
AU - Okorie, N.
AU - Obeagu, E. I.
AU - Nnamani, A. D.
AU - Ude, U. A.
AU - Agada, U. N.
AU - Obi, I. A.
AU - Ibiam, G. A.
DO - 10.9734/JPRI/2021/v33i49B33359
IS - 49
KW - Biological Markers
Oxidative Stress
Lung
Codeine
Cough
PY - 2021
SN - 2456-9119
SP - 228-240
ST - Histopathological Effect of Emzolyn Codein Cough Syrup on Lungs and Its
Oxidative Stress Biomarkers
TI - Histopathological Effect of Emzolyn Codein Cough Syrup on Lungs and Its
Oxidative Stress Biomarkers
VL - 33
ID - 9080
ER -
TY - JOUR
AB - Zinc oxide particles were synthesized in various sizes and shapes, i.e.,
spheres of 40-nm, 200-nm, and 500-nm diameter and rods of 40 center dot 100 nm(2)
and 100 center dot 400 nm(2) (all PVP-stabilized and well dispersed in water and
cell culture medium). Crystallographically, the particles consisted of the
hexagonal wurtzite phase with a primary crystallite size of 20 to 100 nm. The
particles showed a slow dissolution in water and cell culture medium (both neutral;
about 10% after 5 days) but dissolved within about 1 h in two different simulated
lysosomal media (pH 4.5 to 4.8). Cells relevant for respiratory exposure (NR8383
rat alveolar macrophages) were exposed to these particles in vitro. Viability,
apoptosis, and cell activation (generation of reactive oxygen species, ROS, release
of cytokines) were investigated in an in vitro lung cell model with respect to the
migration of inflammatory cells. All particle types were rapidly taken up by the
cells, leading to an increased intracellular zinc ion concentration. The
nanoparticles were more cytotoxic than the microparticles and comparable with
dissolved zinc acetate. All particles induced cell apoptosis, unlike dissolved zinc
acetate, indicating a particle-related mechanism. Microparticles induced a stronger
formation of reactive oxygen species than smaller particles probably due to higher
sedimentation (cell-to-particle contact) of microparticles in contrast to
nanoparticles. The effect of particle types on the cytokine release was weak and
mainly resulted in a decrease as shown by a protein microarray. In the particle-
induced cell migration assay (PICMA), all particles had a lower effect than
dissolved zinc acetate. In conclusion, the biological effects of zinc oxide
particles in the sub-toxic range are caused by zinc ions after intracellular
dissolution, by cell-to-particle contacts, and by the uptake of zinc oxide
particles into cells. Graphical headlights center dot The cytotoxicity of zinc
oxide particles is mainly due to the intracellular release of zinc ions. center dot
The size and shape of zinc oxide micro- and nanoparticles has only small effects on
lung cells in the sub-toxic range. center dot Zinc oxide particles are rapidly
taken up by cells, regardless of their size and shape. center dot Zinc oxide
particles rapidly dissolve after cellular uptake in endolysosomes.
AN - rayyan-553781130
AU - Olejnik, M.
AU - Kersting, M.
AU - Rosenkranz, N.
AU - Loza, K.
AU - Breisch, M.
AU - Rostek, A.
AU - Prymak, O.
AU - Schurmeyer, L.
AU - Westphal, G.
AU - Koller, M.
AU - Bunger, J.
AU - Epple, M.
AU - Sengstock, C.
DO - 10.1007/s10565-020-09571-z
IS - 4
PY - 2021
SN - 0742-2091 1573-6822
SP - 573-593
ST - Cell-biological effects of zinc oxide spheres and rods from the nano- to the
microscale at sub-toxic levels
T2 - CELL BIOLOGY AND TOXICOLOGY
TI - Cell-biological effects of zinc oxide spheres and rods from the nano- to the
microscale at sub-toxic levels
VL - 37
Y2 - 8
ID - 9081
ER -
TY - JOUR
AB - ABSTRACT Diabetes mellitus (DM) is a disease associated with delayed wound
healing of oral ulcers by increased expression of proinflammatory cytokines and
cellular apoptosis. Objective to evaluate the influence of Tumor Necrosis Factor
alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or
triamcinolone. Material and Methods Wistar male rats (210.0±4.2 g) were divided
into five groups: negative control group (NCG) without diabetes; positive control
group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract
(normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG).
Traumatic ulcers were performed on all animals that received topical triamcinolone,
chamomile extract or saline 12/12 hours for ten days. Results On days five and ten
the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL
assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG
(p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day,
however, there was no healing on the TG (p=0.5127) group. The TNF-α expression
showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and
DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant
reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and
in the epithelium only in CDG (p=0.0320). Conclusions Chamomile extract can
optimize the healing of traumatic oral ulcers in diabetic rats through the
reduction of apoptosis in the epithelium and TNF-α expression.
AN - rayyan-553781132
AU - Oliveira, Bruna Vasconcelos
AU - Barros Silva, Paulo Goberlânio
AU - Nojosa, Jacqueline de Santiago
AU - Brizeno, Luiz André Cavalcante
AU - Ferreira, Jamile Magalhães
AU - Sousa, Fabrício Bitú
AU - Mota, Mário Rogério Lima
AU - Alves, Ana Paula Negreiros Nunes
DO - 10.1590/1678-775720150481
IS - 3
KW - Chamomile
Diabetes mellitus
Matricaria
Oral ulcer
Triamcinolone
Rats
LA - en
PY - 2016
SN - 1678-7757
SP - 278-290
ST - TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria
recutita L. extract and triamcinolone on oral ulcer in diabetic rats
T2 - J. appl. oral sci
TI - TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria
recutita L. extract and triamcinolone on oral ulcer in diabetic rats
77572016000300278
VL - 24
Y2 - 6 y3 - 1
ID - 9083
ER -
TY - GEN
AB - Os nódulos de tireóide são frequentes no mundo todo, principalmente entre as
mulheres. A preocupação maior no seu estudo é afastar a presença de neoplasia
maligna, que corresponde a aproximadamente 10% de todos os casos. Alguns
procedimentos médicos têm sido usados para se chegar ao diagnóstico pré-operatório
dos nódulos tireoideanos, dentre os quais a cintilografia, a ultrassonografia e,
mais recentemente, a punção aspirativa com agulha fina... Na tentativa de colaborar
para o esclarecimento desse tema, estudamos 102 amostras de tireóide arquivadas no
banco de tumores do Hospital AC Camargo, formando com elas dois grupos de
pacientes, um com carcinoma e tireoidite (70 pacientes) e outro, somente com
carcinoma (32 pacientes). Tendo em vista a importância da caracterização objetiva
do fenômeno inflamatório, subclassificamos, por parâmetros histológicos, a
tireoidite em leve, moderada e intensa. Foi realizado estudo imunoistoquímico para
avaliação de 16 proteínas relacionadas à presença de células indiferenciadas (p63),
à via das MAPKs (Ras, AKT-1e ERK1/2), às moléculas de adesão (E-caderina e CD44), à
ativação da via de sinalização Wnt (beta-catenina), à via do receptor de morte
(Fas-L e caspase 8), às moléculas ligadas à indução de interleucinas (iNOS e COX-
2), aos fatores de crescimento e diferenciação celulares (galectina 3 e VEGF) e aos
índices de proliferação celular e apoptose (Ki-67, caspase 3 clivada e Fas). Além
disso, fizemos a pesquisa da mutação V600E do gene BRAF por pirossequenciamento e a
pesquisa dos rearranjos cromossômicos RET/PTC1 e RET/PTC3 por RT-PCR. Os resultados
evidenciaram diferenças estatisticamente significativas na expressão de Ras,
ERK1/2, CD44, COX-2 e Fas entre os grupos com e sem tireoidite. Essas diferenças
também validaram a subclassificação histológica para a intensidade da tireoidite,
ao demonstrar que quanto mais intensa ela se apresentou, maior foi a expressão
imunoistoquímica dessas proteínas. Thyroid nodules are common throughout the world,
mainly in women. The principal focus of their study is to exclude the possibility
of a malignant neoplasm, which is found in approximately 10% of all cases. Various
medical procedures, such as scintillography, ultrasonography, and, more recently,
fine needle aspiration biopsy are used pre-operatively to diagnose thyroid nodules.
However, the definitive diagnosis is still the pathologic diagnosis, often preceded
by examination of multiple frozen sections. One of the limiting factors of this
method is the presence of autoimmune thyroiditis, as the morphological tissue
alterations associated with this inflammation cause problems in the macroscopic and
microscopic evaluation of the tissue. Not only during the frozen section analysis,
but also during the definitive evaluation, the association between thyroiditis and
papillary carcinoma is a challenge, as there is still no consensus about the nature
of this association. In an attempt to help clarify this situation, we studied
samples of thyroid tissue from 102 patients, collected from the archives of the
Tumor Bank of the AC Camargo Hospital, divided into two groups; the first group
consisted of 70 patients with both carcinoma and thyroiditis, while the second
group of 32 patients had only carcinoma. Due to the importance of an objective
characterization of the inflammatory process, specifically in this research,
histological parameters were used to sub classify the thyroiditis as mild, moderate
or severe. We used immunihistochemical methods to study 16 proteins related to: the
presence of undifferentiated cells (p63); the MAPK pathway (Ras, AKT-1 and ERK1/2);
adhesion molecules (E-caderin and CD44); the Wnt signal activation pathway (beta-
catenin); the death receptor pathway (Fas-L and caspase 8); molecules associated
with induction of interleukins (iNOS and COX-2); factors of growth and cellular
differentiation (Galactin 3 and VEGF); and indices of cellular proliferation and
apoptosis (Ki67, activated caspase 3 and Fas). As we l, we used pyrosequencing to
study the V600E mutation of the BRAF gene, and RT-PCR to evaluate rearrangements of
chromosomes RET/PTC1 and RET/PTC3. Our results showed statistically significant
differences between the groups with and without thyroiditis in the expression of
Ras, ERK 1/2, CD44, COX-2 and Fas. These results also validated the histological
sub classification used to grade the intensity of the thyroiditis; the more intense
the thyroiditis, the greater was the immunihistochemical expression of these
proteins.
AN - rayyan-553781133
AU - Oliveira, Paulo Roberto Grimaldi
LA - pt
PY - 2009
SP - 137-137
ST - Carcinoma papilífero da tireóide: estudo comparativo entre os casos usuais e
aqueles associados à tireoidite autoimune
TI - Carcinoma papilífero da tireóide: estudo comparativo entre os casos usuais e
aqueles associados à tireoidite autoimune
UR - http://accamargo.phlnet.com.br/Doutorado/2009/PGrimaldi/PGrimaldi.pdf
ID - 9084
ER -
TY - JOUR
AB - Zinc (Zn) has emerged as a promising bioresorbable stent material because of
its satisfactory corrosion behavior and excellent biocompatibility. However, for
load-bearing implant applications, alloying is required to boost its mechanical
properties as pure Zn exhibits poor strength. Unfortunately, an increase in
inflammation relative to pure Zn is a commonly observed side effect of Zn alloys.
Consequently, the development of a Zn-based alloy that can simultaneously feature
improved mechanical properties and suppress inflammatory responses is a big
challenge. Here, a bioresorbable, biocompatible Zn-Ag-based quinary alloy was
comprehensively evaluated in vivo, in comparison to reference materials. The
inflammatory and smooth muscle cellular response was characterized and correlated
to metrics of neointimal (NI) growth. We found that implantation of the quinary
alloy was associated with significantly improved inflammatory activities relative
to the reference materials. Additionally, we found that inflammation, but not
smooth muscle cell hyperplasia, significantly correlates to NI growth for Zn
alloys. The results suggest that inflammation is the main driver of NI growth for
Zn-based alloys and that the quinary Zn-Ag-Mn-Zr-Cu alloy may impart inflammation-
resistance properties to arterial implants. © 2020 American Chemical Society.
AN - rayyan-553781134
AU - Oliver, A. A.
AU - Guillory, R. J.
AU - Flom, K. L.
AU - Morath, L. M.
AU - Kolesar, T. M.
AU - Mostaed, E.
AU - Sikora-Jasinska, M.
AU - Drelich, J. W.
AU - Goldman, J.
DO - 10.1021/acsabm.0c00740
IS - 10
biodegradable stent
inflammation
neointima
zinc alloy
Biocompatibility
Cells
Copper alloys
Corrosive effects
Manganese alloys
Mechanical properties
Metal implants
Muscle
Pathology
Silver alloys
Zircaloy
Cellular response
Corrosion behavior
Inflammatory activity
Reference material
Resistance properties
Smooth muscle cells
Zn-based alloys
Zinc alloys
Inflammation
PY - 2020
SP - 6779-6789
ST - Analysis of Vascular Inflammation against Bioresorbable Zn-Ag-Based Alloys
T2 - ACS Applied Bio Materials
TI - Analysis of Vascular Inflammation against Bioresorbable Zn-Ag-Based Alloys
85096334801&doi=10.1021%2facsabm.0c00740&partnerID=40&md5=a632335302fba9d67d11f17fa
a589e47
VL - 3
ID - 9085
ER -
TY - JOUR
AB - Silver nanoparticles are well received in the cosmeceutical industry due to
their broad spectrum of pharmacology applications. Research on the therapeutic
properties exhibited by silver nanoparticles revealed that the antimicrobial and
anti-inflammatory properties are the main attraction in the establishment of
nanocosmeceutical products whereby their mechanisms of action are reviewed in this
paper. In addition, studies on other uses of silver nanoparticles acknowledged that
the particles act as antifungal agents in nail polishes and pigments in coloured
beauty products such as lipsticks and eye shadows. Despite the extensive use of
silver nanoparticles in the cosmetic line, there are still limited resources on the
mechanism of actions and the effect of the particles on the bio-functionality of
the body. The safety of silver nanoparticles could be comprehended from their skin
penetration ability and toxicity to the human body in which it could be justified
that both features are mainly influenced by the morphology of the particles and the
method of application. This article summarizes exclusively on the synthesis of
silver nanoparticles, the biomedical mechanisms and applications as well the
limitations with respect to skin penetration ability and toxicity effects which
will contribute significantly to the vast research on the association of
nanotechnology and cosmetics. (C) 2022 Published by Elsevier B.V. on behalf of King
Saud University.
AN - rayyan-553781140
AU - Ong, W. T. J.
AU - Nyam, K. L.
DO - 10.1016/j.sjbs.2022.01.035
IS - 4
PY - 2022
SN - 1319-562X 2213-7106
SP - 2085-2094
ST - Evaluation of silver nanoparticles in cosmeceutical and potential biosafety
complications
TI - Evaluation of silver nanoparticles in cosmeceutical and potential biosafety
complications
VL - 29
Y2 - 4
ID - 9091
ER -
TY - JOUR
AB - Purpose: To investigate the effects of melatonin on antioxidant capacity,
inflammation and apoptotic cell death (through expression of cleaved-caspase 3) in
lung tissue samples of diabetic rats. Methods: Thirty male Sprague-Dawley rats were
randomly divided into three groups. Group 1 (control group) was made up of healthy
rats. Group 2 (diabetes group) received streptozotocin at a dose of 50 mg/kg/day
for 5 days.Group 3 (diabetes plus melatonin group) received streptozotocin at a
dose of 50 mg/kg/day for 5 days and then they received melatonin at a dose of 20
mg/kg/day between 28thand 35thdays of the study. Results: Tissue MDA and MPO levels
were found to be significantly higher in diabetes group compared to control group
(p<0.05) whilst administration of melatonin was found to significantly lower this
increase down to normal levels (p<0.05). Bronchus associated lymphoid tissue (BALT)
was more severe in diabetics whereas administration of melatonin alleviated this
hyperplasia. Cleaved caspase 3 activity was severe in hyperplastic BALT in diabetic
rats however in lowered down to moderate level when melatonin was administered.
Conclusion: The melatonin caused an increase in antioxidant capacity and decreased
the expression of cleaved-caspase 3.
AN - rayyan-553781141
AU - Onk, Didem
AU - Onk, Oruç Alper
AU - Erol, Hüseyin Serkan
AU - Özkaraca, Mustafa
AU - Çomakl
AU - Selim
AU - Ayazo
AU - lu, Tülin Akarsu
AU - Kuyrukluy
AU - ld
AU - z, Ufuk
AU - Ünver, Süheyla
DO - 10.1590/s0102-865020180040000009
IS - 4
KW - Lung
Melatonin
Rats.
Rats
Inflammation
Antioxidants
LA - en
PY - 2018
SN - 0102-8650
SP - 375-385
ST - Effect of melatonin on antioxidant capacity, inflammation and apoptotic cell
death in lung tissue of diabetic rats
T2 - Acta cir. bras
TI - Effect of melatonin on antioxidant capacity, inflammation and apoptotic cell
death in lung tissue of diabetic rats
86502018000400375
VL - 33
Y2 - 4 y3 - 1
ID - 9092
ER -
TY - CHAP
AB - Since the advances in nanotechnology of a few decades ago, numerous
nanoparticles (NPs) have been manufactured and used throughout the world. NPs have
varied technological applications in electronics, photonics, medical, garments,
sporting goods, agricultural products and clean energy. Because the amount of NPs
to which humans are exposed is likely increasing, the health effects of NP exposure
and their underlying mechanism are being investigated. In order to utilize NPs more
safely, the means by which their negative effects are reduced must be discovered.
Previous studies have reported that various types of NPs, such as carbon black,
carbon nanotubes, silver, nickel, silica, silicon, and various metal oxides induce
reactive oxygen species (ROS), which cause oxidative stress and cell damage, to all
life forms (plants to mammals) regardless of species. For example, when single-wall
carbon nanotubes are intratracheally administered to rats (0.2 and 2.0 mg/kg/day,
once every three days, a total of 14 instillations), ROS in the lung are increased
and the exacerbation of allergic asthma is induced. Intravenous injection of
single-wall carbon nanotube suspension (6.25 and 12.5 mg/kg/day for 9 consecutive
days) induces oxidative stress in the brain and decreases locomotor activity in
mice. These reports demonstrate that the generation of ROS is one of the most
important factors in the mechanism of NP health effects. These studies also showed
the possibility of protective effects of antioxidants such as vitamin C (ascorbic
acid) and vitamin E (tocopherol) on NP-induced oxidative stress. The depletion of
antioxidants exacerbated the inflammatory response to NP exposure. Because
antioxidants reduce the effects of NPs via ROS elimination, these studies will
contribute to the safe and practical use of NPs. Some evidence suggests that
oxidative stress may be a starting point for the main mechanism underlying the NP
toxicity. According to the hierarchical oxidative stress hypothesis, the lowest
level of oxidative stress is associated with cytoprotective responses, such as the
induction of antioxidant and detoxification enzymes. If the level of protection
fails, the oxidative stress will lead to proinflammatory effects. Further
escalation will trigger disturbances in mitochondrial function, resulting in
cellular apoptosis or necrosis. Both endogenous and dietary antioxidants can be
considered as a first line of defense against the generation of ROS due to NP
exposure. Accordingly, it is highly possible that antioxidants may act as a
protective agent against damage induced by various types of NPs through their
downregulation of the oxidative stress level. © 2015 Nova Science Publishers, Inc.
AN - rayyan-553781142
AU - Onoda, A.
AU - Umezawa, M.
AU - Takeda, K.
KW - Antioxidant
Ascorbic acid
Nanoparticle
Omega-3 polyunsaturated fatty acid
Oxidative stress
α-tocopherol
Antioxidants
PY - 2015
SP - 197-210
ST - The potential protective effect of antioxidants on nanoparticle toxicity
T2 - PM2.5: Role of Oxidative Stress in Health Effects and Prevention Strategy
TI - The potential protective effect of antioxidants on nanoparticle toxicity
84956814695&partnerID=40&md5=46bc66952b2ebe7c2b20490da3565683
ID - 9093
ER -
TY - JOUR
AB - Background: With the increasing use of nanomaterials, the need for methods
and assays to examine their immunosafety is becoming urgent, in particular for
nanomaterials that are deliberately administered to human subjects (as in the case
of nanomedicines). To obtain reliable results, standardised in vitro
immunotoxicological tests should be used to determine the effects of engineered
nanoparticles on human immune responses. However, before assays can be
standardised, it is important that suitable methods are established and
validated.Results: In a collaborative work between European laboratories, existing
immunological and toxicological in vitro assays were tested and compared for their
suitability to test effects of nanoparticles on immune responses. The prototypical
nanoparticles used were metal (oxide) particles, either custom-generated by wet
synthesis or commercially available as powders. Several problems and challenges
were encountered during assay validation, ranging from particle agglomeration in
biological media and optical interference with assay systems, to chemical
immunotoxicity of solvents and contamination with endotoxin.Conclusion: The
problems that were encountered in the immunological assay systems used in this
study, such as chemical or endotoxin contamination and optical interference caused
by the dense material, significantly affected the data obtained. These problems
have to be solved to enable the development of reliable assays for the assessment
of nano-immunosafety. © 2011 Oostingh et al; licensee BioMed Central Ltd.
AN - rayyan-553781143
AU - Oostingh, G. J.
AU - Casals, E.
AU - Italiani, P.
AU - Colognato, R.
AU - Stritzinger, R.
AU - Ponti, J.
AU - Pfaller, T.
AU - Kohl, Y.
AU - Ooms, D.
AU - Favilli, F.
AU - Leppens, H.
AU - Lucchesi, D.
AU - Rossi, F.
AU - Nelissen, I.
AU - Thielecke, H.
AU - Puntes, V. F.
AU - Duschl, A.
AU - Boraschi, D.
DO - 10.1186/1743-8977-8-8
KW - Animals
Biological Assay
Cells
Cells, Cultured
Humans
Immunologic Factors
Interleukin-8
Metal Nanoparticles
Promoter Regions, Genetic
Solvents
adenylate kinase
cerium oxide
cobalt oxide
cytokine
endotoxin
gold
iron oxide
metal
metal oxide
nanoparticle
RNA
silver
silver oxide
solvent
unclassified drug
biomaterial
immunologic factor
interleukin 8
metal nanoparticle
article
biocompatibility
cell activation
cell assay
cell damage
cell proliferation
cell strain CACO 2
cell viability
contamination
controlled study
cytokine production
enzyme assay
enzyme release
gene expression
genetic transfection
genotoxicity
human
human cell
human cell culture
immune response
immunomodulation
immunotoxicity
in vitro study
inflammatory cell
monocyte
optics
powder
priority journal
promoter region
reliability
RNA extraction
safety
toxicity testing
validation study
animal
bioassay
cell
cell culture
chemistry
cytology
genetics
immunology
metabolism
methodology
reproducibility
standard
Humanities
Humanism
PY - 2011
ST - Problems and challenges in the development and validation of human cell-based
assays to determine nanoparticle-induced immunomodulatory effects
TI - Problems and challenges in the development and validation of human cell-based
assays to determine nanoparticle-induced immunomodulatory effects
79651474507&doi=10.1186%2f1743-8977-8-
8&partnerID=40&md5=b0613732505a7d252457840c59ba2a67
VL - 8
ID - 9094
ER -
TY - JOUR
AB - BACKGROUND: The exact mechanism of knee osteoarthritis (OA)-associated pain
is unclear, whereas mixed evidence of inflammatory pain and neuropathic pain has
been noted. We aimed to investigate pain-related sensory innervation in a
monoiodoacetate (MIA)-induced model of OA. METHODS: Sixty of seventy female Sprague
Dawley rats of six week-old underwent intra-articular MIA and fluorogold (FG)
retrograde neurotracer injection into their right (ipsilateral) knee, while their
left knees were treated with FG in saline as a control (contralateral knee). Other
rats were treated with FG only bilaterally, and used as controls. Rats were
evaluated for tactile allodynia using von Frey hairs. Proinflammatory mediators in
the knee soft tissues, including tumor necrosis factor (TNF)-α, interleukin (IL)-6,
and nerve growth factor (NGF), were quantified using ELISAs to evaluate
inflammation in the knee after 1, 4, 7, 14, 21, and 28 days post injection. Dorsal
root ganglia (DRG) were immunostained for three molecules after 7, 14, 21, and 28
days post injection: calcitonin gene-related peptide (CGRP), a marker of
inflammatory pain; and activating transcription factor-3 (ATF3) and growth
associated protein-43 (GAP43), as markers for nerve injury and regenerating axons.
The distribution of microglia in the spinal cord were also evaluated, because they
have been reported to increase in neuropathic pain states. These evaluations were
performed up to 28 days postinjection. P < 0.05 was considered significant.
RESULTS: Progressive tactile allodynia and elevated cytokine concentrations were
observed in ipsilateral knees. CGRP-immunoreactive (-ir) ipsilateral DRG neurons
significantly increased, peaking at 14 days postinjection, while expression of FG-
labeled ATF3-ir or ATF3-ir GAP43-ir DRG neurons significantly increased in a time-
dependent manner. Significant proliferation of microglia were found with time in
the ipsilateral dorsal horn. CONCLUSIONS: Pain-related characteristics in a MIA-
induced rat OA model can originate from an inflammatory pain state induced by the
local inflammation initiated by inflammatory cytokines, and that state will be
followed by gradual initiation of neuronal injury, which may induce the neuropathic
pain state.
AN - rayyan-553782310
AU - Orita, S.
AU - Ishikawa, T.
AU - Miyagi, M.
AU - Ochiai, N.
AU - Inoue, G.
AU - Eguchi, Y.
AU - Kamoda, H.
AU - Arai, G.
AU - Toyone, T.
AU - Aoki, Y.
AU - Kubo, T.
AU - Takahashi, K.
AU - Ohtori, S.
DO - 10.1186/1471-2474-12-134
J2 - BMC Musculoskelet Disord
KW - Animals
Arthralgia/etiology/*pathology/physiopathology
Chronic Disease
Disease Progression
Female
Inflammation/chemically induced/pathology/physiopathology
Inflammation Mediators/*toxicity
Iodoacetic Acid/*toxicity
Longitudinal Studies
Nerve Degeneration/etiology/pathology/physiopathology
Osteoarthritis, Knee/complications/*pathology/physiopathology
Peripheral Nervous System Diseases/etiology/pathology/physiopathology
Rats
Sensory Receptor Cells/metabolism/*pathology
Osteoarthritis
LA - eng
N1 - Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba
University, Chiba, Japan. sumihisa@silver.email.ne.jp
PY - 2011
SP - 134
ST - Pain-related sensory innervation in monoiodoacetate-induced osteoarthritis in
rat knees that gradually develops neuronal injury in addition to inflammatory pain
T2 - BMC musculoskeletal disorders
TI - Pain-related sensory innervation in monoiodoacetate-induced osteoarthritis in
rat knees that gradually develops neuronal injury in addition to inflammatory pain
VL - 12
Y2 - 6 y3 - 16
ID - 10218
ER -
TY - JOUR
AB - The interaction between silver nanoparticles and herpesviruses is attracting
great interest due to their antiviral activity and possibility to use as
microbicides for oral and anogenital herpes. In this work, we demonstrate that
tannic acid modified silver nanoparticles sized 13 nm, 33 nm and 46 nm are capable
of reducing HSV-2 infectivity both in vitro and in vivo. The antiviral activity of
tannic acid modified silver nanoparticles was size-related, required direct
interaction and blocked virus attachment, penetration and further spread. All
tested tannic acid modified silver nanoparticles reduced both infection and
inflammatory reaction in the mouse model of HSV-2 infection when used at infection
or for a post-infection treatment. Smaller-sized nanoparticles induced production
of cytokines and chemokines important for anti-viral response. The corresponding
control buffers with tannic acid showed inferior antiviral effects in vitro and
were ineffective in blocking in vivo infection. Our results show that tannic acid
modified silver nanoparticles are good candidates for microbicides used in
treatment of herpesvirus infections.
AN - rayyan-553781147
AU - Orlowski, P.
AU - Tomaszewska, E.
AU - Gniadek, M.
AU - Baska, P.
AU - Nowakowska, J.
AU - Sokolowska, J.
AU - Nowak, Z.
AU - Donten, M.
AU - Celichowski, G.
AU - Grobelny, J.
AU - Krzyzowska, M.
DO - 10.1371/journal.pone.0104113
IS - 8
KW - Herpes Simplex
PY - 2014
SN - 1932-6203
ST - Tannic Acid Modified Silver Nanoparticles Show Antiviral Activity in Herpes
Simplex Virus Type 2 Infection
T2 - PLOS ONE
TI - Tannic Acid Modified Silver Nanoparticles Show Antiviral Activity in Herpes
Simplex Virus Type 2 Infection
VL - 9
Y2 - 8 y3 - 12
ID - 9098
ER -
TY - JOUR
AB - INTRODUCTION: Silver nanoparticles (AgNPs) have been shown to promote wound
healing and to exhibit antimicrobial properties against a broad range of bacteria.
In our previous study, we prepared tannic acid (TA)-modified AgNPs showing a good
toxicological profile and immunomodulatory properties useful for potential dermal
applications. METHODS: In this study, in vitro scratch assay, antimicrobial tests,
modified lymph node assay as well as a mouse splint wound model were used to access
the wound healing potential of TA-modified and unmodified AgNPs. RESULTS: TA-
modified but not unmodified AgNPs exhibited effective antibacterial activity
against Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli and
stimulated migration of keratinocytes in vitro. The tests using the mouse splint
wound model showed that TA-modified 33 and 46 nm AgNPs promoted better wound
closure, epithelialization, angiogenesis and formation of the granulation tissue.
Additionally, AgNPs elicited expression of VEGF-α, PDGF-β and TGF-β1 cytokines
involved in wound healing more efficiently in comparison to control and TA-treated
wounds. However, both the lymph node assay and the wound model showed that TA-
modified AgNPs sized 13 nm can elicit strong inflammatory response not only during
wound healing but also when applied to the damaged skin. CONCLUSION: TA-modified
AgNPs sized >26 nm promote wound healing better than TA-modified or unmodified
AgNPs. These findings suggest that TA-modified AgNPs sized >26 nm may have a
promising application in wound management.
AN - rayyan-553781997
AU - Orlowski, P.
AU - Zmigrodzka, M.
AU - Tomaszewska, E.
AU - Ranoszek-Soliwoda, K.
AU - Czupryn, M.
AU - Antos-Bielska, M.
AU - Szemraj, J.
AU - Celichowski, G.
AU - Grobelny, J.
AU - Krzyzowska, M.
DO - 10.2147/IJN.S154797
KW - Animals
Cell Line
Dermis/pathology
Dynamic Light Scattering
Endocytosis/drug effects
Female
Fibroblasts/drug effects/metabolism
Humans
Inflammation/pathology
Keratinocytes/drug effects/metabolism
Metal Nanoparticles/*chemistry/toxicity/ultrastructure
Mice
Mice, Inbred C57BL
Monocytes/drug effects/metabolism
Neovascularization, Physiologic/drug effects
*Particle Size
RAW 264.7 Cells
Silver/*chemistry
Tannins/*chemistry
*Wound Healing
Wound Healing
LA - eng
N1 - Military Institute of Hygiene and Epidemiology, Warsaw, Poland.; Department
of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw
University of Life Sciences (WULS-SGGW), Warsaw, Poland.; Department of Materials
Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lodz, Poland.;
Department of Materials Technology and Chemistry, Faculty of Chemistry, University
of Lodz, Lodz, Poland.; Military Institute of Hygiene and Epidemiology, Warsaw,
Poland.; Military Institute of Hygiene and Epidemiology, Warsaw, Poland.;
Bionanopark, Lodz, Poland.; Department of Materials Technology and Chemistry,
Faculty of Chemistry, University of Lodz, Lodz, Poland.; Department of Materials
Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lodz, Poland.;
Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
PY - 2018
SP - 991-1007
ST - Tannic acid-modified silver nanoparticles for wound healing: the importance
of size
TI - Tannic acid-modified silver nanoparticles for wound healing: the importance
of size
VL - 13
ID - 9914
ER -
TY - JOUR
AB - BackgroundSilver ions from silver nanoparticles (AgNP) or AgNPs themselves
itself that are ingested from consumer health care products or indirectly from
absorbed food contact material can interact with the gastrointestinal tract (GIT).
The permeability of the GIT is strictly regulated to maintain barrier function and
proper nutrient absorption. The single layer intestinal epithelium adheres and
communicates actively to neighboring cells and the extracellular matrix through
different cell junctions. In the current study, we hypothesized that oral exposure
to AgNPs may alter the intestinal permeability and expression of genes controlling
cell junctions. Changes in cell junction gene expression in the ileum of male and
female rats administered different sizes of AgNP for 13-weeks were assessed using
qPCR.ResultsThe results of this study indicate that AgNPs have an altering effect
on cell junctions that are known to dictate intestinal permeability. mRNA
expression of genes representing tight junction (Cldn1, Cldn5, Cldn6, Cldn10 and
Pecam1), focal adhesion (Cav1, Cav2, and Itgb2), adherens junction (Pvrl1, Notch1,
and Notch2), and hemidesmosome (Dst) groups were upregulated significantly in
females treated with 10nm AgNP, while no change or downregulation of same genes was
detected in male animals. In addition, a higher concentration of pro-inflammatory
cytokine, TNF-, was noticed in AgNP-treated female animals as compared to
controls.ConclusionsThis study proposes that interaction of silver with GIT could
potentially initiate an inflammatory process that could lead to changes in the
gastrointestinal permeability and/or nutrient deficiencies in sex-specific manner.
Fully understanding the mechanistic consequences of oral AgNP exposure may lead to
stricter regulation for the commercial usage of AgNPs and/or improved clinical
therapy in the future.
AN - rayyan-553781149
AU - Orr, S. E.
AU - Gokulan, K.
AU - Boudreau, M.
AU - Cerniglia, C. E.
AU - Khare, S.
DO - 10.1186/s12951-019-0499-6
KW - Rats
Permeability
Gene Expression
RNA, Messenger
PY - 2019
SN - 1477-3155
ST - Alteration in the mRNA expression of genes associated with gastrointestinal
permeability and ileal TNF- secretion due to the exposure of silver nanoparticles
in Sprague-Dawley rats
TI - Alteration in the mRNA expression of genes associated with gastrointestinal
permeability and ileal TNF- secretion due to the exposure of silver nanoparticles
in Sprague-Dawley rats
VL - 17
Y2 - 5 y3 - 13
ID - 9100
ER -
TY - JOUR
AB - This study investigated the effects of topical application of chitosan-capped
silver nanoparticles (Ch/AgNPs) on burn wound healing. The chitosan-capped silver
nanoparticles were synthesized in one step from the silver nitrate, sodium
borohydride, and chitosan and were characterized using transmission electron
microscopy, fourier transform infrared spectroscopy, and X-ray diffraction methods.
The antioxidant assay was performed to evaluate the scavenging rate. The effects of
Ch/AgNPs on burn wound healing was also evaluated by histopathological, molecular,
and biochemical evaluations after 7, 14 and 28 days of treatment in a rat model. In
comparison to the negative control and silver sulfadiazine groups, the Ch/AgNPs
treated wounds exhibited significantly lower inflammatory reaction as determined by
the reduced level of interleukin-1 beta (IL-1 beta) and neutrophil counts.
Treatment by Ch/AgNPs also significantly enhanced re-epithelialization, so that
complete epithelialization was achieved in the lesions of the animals of this
group, at the 7th day post-wounding. Rapid reepithelialization, improved
granulation tissue formation, reduced IL-1 beta expression, mild inflammation, and
increased transforming growth factor-beta 1 and basic fibroblast growth factor, at
7 days post-wounding, are convincing reasons to confirm this idea that Ch/AgNPs are
effective in speeding up the wound healing stages. Our histopathological findings
are in agreement with the molecular and biochemical results and strongly
demonstrate that Ch/AgNPs stimulate burn wound healing by decreasing the length of
repair phases. Therefore, on the basis of our findings, Ch/AgNPs can be a promising
candidate in stimulating wound repair and regeneration.
AN - rayyan-553781151
AU - Oryan, A.
AU - Alemzadeh, E.
AU - Tashkhourian, J.
AU - Ana, S. F. N.
DO - 10.1016/j.carbpol.2018.07.077
KW - Burns
PY - 2018
SN - 0144-8617 1879-1344
SP - 82-92
ST - Topical delivery of chitosan-capped silver nanoparticles speeds up healing in
burn wounds: A preclinical study
T2 - CARBOHYDRATE POLYMERS
TI - Topical delivery of chitosan-capped silver nanoparticles speeds up healing in
burn wounds: A preclinical study
VL - 200
Y2 - 11 y3 - 15
ID - 9101
ER -
TY - JOUR
AB - Environmental research has been significantly enriched by genomic and
proteomic methodologies. Particularly in the atmospheric pollution studies, where a
variety of environmental hazards act simultaneously and frequently in close
interrelationship, only such integrative methods can provide a comprehensive
analysis. Nanoparticles are a heterogeneous class of particles, but their health
effects have also common aspects. The scope of the article is to present the
contribution of genomics and proteomics in extending the knowledge about the
biological effects of atmospheric nanoparticles in humans: how they interact with
genes and genes' expression, how they affect translation and cell proteome and how
they interfere with epigenetic mechanisms. Different methods, with advantages and
disadvantages, and main contribution are described. Most studies underline the
oxidative stress and the inflammatory mechanisms induced by nanoparticles at the
bronchial epithelium level and at the systemic level. Oxidative mechanism is
investigated with genomic technics and confirmed in proteomic studies and in
epigenetic ones. These techniques have higher sensitivity in identifying parameters
related to cytoxicity of different human cells than other biological measurements
and an increasing role in evaluating environmental management and interventions.
AN - rayyan-553781153
AU - Otelea, M.
AU - Rascu, A.
DO - 10.30638/eemj.2015.243
IS - 10
KW - Genome
Genomics
Proteomics
Proteome
PY - 2015
SN - 1582-9596 1843-3707
SP - 2283-2291
ST - GENOMICS AND PROTEOMICS TECHNIQUES IN NANOPARTICLES STUDIES - NEW APPROACH IN
ENVIRONMENTAL RESEARCH
T2 - ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL
TI - GENOMICS AND PROTEOMICS TECHNIQUES IN NANOPARTICLES STUDIES - NEW APPROACH IN
ENVIRONMENTAL RESEARCH
VL - 14
Y2 - 10
ID - 9103
ER -
TY - JOUR
AB - After a biomaterial is implanted in a bone defect area, the immune response
and bacterial infection affect the success of bone regeneration. In this study, we
describe the development of a promising therapeutic approach to accelerate bone
regeneration via combining osteoimmunomodulatory and antibacterial activities.
Herein, we fabricated a nanosilver/halloysite nanotubes/gelatin methacrylate
(nAg/HNTs/GelMA) hybrid hydrogel and evaluated its osteoimmunomodulatory and
antibacterial properties in vitro and in vivo. The nAg/HNTs/GelMA hybrid hydrogel
had good biocompatibility with human periodontal ligament stem cells (hPDLSCs) and
macrophages. Moreover, the nAg/HNTs/GelMA hybrid hydrogel modulated inflammatory
cytokines secreted by macrophages and enhanced the osteogenic differentiation of
hPDLSCs in an inflammatory environment. In addition, nAg/HNTs/GelMA hybrid hydrogel
inhibited the growth of Gram-positive and Gram-negative bacteria in vitro and in
vivo. Compared with HNTs/GelMA hydrogel, the nAg/HNTs/GelMA hybrid hydrogel better
modulated the osteoimmune microenvironment and eliminated bacterial infection.
Thus, this hybrid hydrogel combining osteoimmunomodulatory with antibacterial
activities is a promising biomaterial for bone regeneration in defect areas.
AN - rayyan-553781154
AU - Ou, Q. M.
AU - Huang, K. Q.
AU - Fu, C. Q.
AU - Huang, C. L.
AU - Fang, Y. F.
AU - Gu, Z. P.
AU - Wu, J.
AU - Wang, Y.
DO - 10.1016/j.cej.2019.123019
KW - Methacrylates
Bone Regeneration
Bone and Bones
Regeneration
PY - 2020
SN - 1385-8947 1873-3212
ST - Nanosilver-incorporated halloysite nanotubes/gelatin methacrylate hybrid
hydrogel with osteoimmunomodulatory and antibacterial activity for bone
regeneration
T2 - CHEMICAL ENGINEERING JOURNAL
TI - Nanosilver-incorporated halloysite nanotubes/gelatin methacrylate hybrid
hydrogel with osteoimmunomodulatory and antibacterial activity for bone
regeneration
VL - 382
Y2 - 2 y3 - 15
ID - 9104
ER -
TY - JOUR
AB - Major problems with biomedical devices in particular implants located in
nonsterile environments concern: (i) excessive immune response to the implant, (ii)
development of bacterial biofilms, and (iii) yeast and fungi infections. An
original multifunctional coating that addresses all these issues concomitantly is
developed. A new exponentially growing polyelectrolyte multilayer film based on
polyarginine (PAR) and hyaluronic acid (HA) is designed. The films have a strong
inhibitory effect on the production of inflammatory cytokines released by human
primary macrophage subpopulations. This could reduce potential chronic inflammatory
reaction following implantation. Next, it is shown that PAR, due to its positive
charges, has an antimicrobial activity in film format against Staphylococcus aureus
for 24 h. In order to have a long-term antimicrobial activity, a precursor
nanoscale silver coating is deposited on the surface before adding the PAR/HA
films. Moreover, the PAR/HA films can be easily further functionalized by embedding
antimicrobial peptides, like catestatin (CAT), a natural host defense peptide. This
PAR/HA+CAT film proves to be effective as an antimicrobial coating against yeast
and fungi and its cytocompatibility is also assessed. Finally, this all-in-one
system constitutes an original strategy to limit inflammation and prevents
bacteria, yeast, and fungi infections.
AN - rayyan-553782235
AU - Özçelik, H.
AU - Vrana, N. E.
AU - Gudima, A.
AU - Riabov, V.
AU - Gratchev, A.
AU - Haikel, Y.
AU - Metz-Boutigue, M. H.
AU - Carradò, A.
AU - Faerber, J.
AU - Roland, T.
AU - Klüter, H.
AU - Kzhyshkowska, J.
AU - Schaaf, P.
AU - Lavalle, P.
DO - 10.1002/adhm.201500546
IS - 13
J2 - Adv Healthc Mater
KW - Anti-Infective Agents/*chemistry/pharmacology
Aspergillus fumigatus/drug effects
Cells, Cultured
Chromogranin A/chemistry/pharmacology
Coated Materials, Biocompatible/*chemistry/pharmacology
Human Umbilical Vein Endothelial Cells
Humans
Hyaluronic Acid/*chemistry
Immobilized Proteins/chemistry/pharmacology
Interferon-gamma/metabolism
Macrophages/cytology/drug effects/immunology
Microscopy, Fluorescence
Nanostructures/chemistry
Peptide Fragments/chemistry/pharmacology
Peptides/*chemistry
Silver/chemistry
Surface Properties
LA - eng
N1 - Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121,
11 rue Humann, 67085, Strasbourg, France.; Faculté de Chirurgie Dentaire,
Université de Strasbourg, 1 Place de l'Hôpital, 67000, Strasbourg, France.;
Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11
rue Humann, 67085, Strasbourg, France.; Protip SAS, 8 Place de l'Hôpital, 67000,
Strasbourg, France.; Institute of Transfusion Medicine and Immunology, Medical
Faculty Mannheim, University of Heidelberg, Theodor-Kutzer Uber 1-3, 68167,
Mannheim, Germany.; Institute of Transfusion Medicine and Immunology, Medical
Mannheim, Germany.; Laboratory for Translational Cellular and Molecular
Biomedicine, Tomsk State University, 36 Lenin Prospekt, Tomsk, 634050, Russia.;
Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11
rue Humann, 67085, Strasbourg, France.; Faculté de Chirurgie Dentaire, Université
de Strasbourg, 1 Place de l'Hôpital, 67000, Strasbourg, France.; Institut National
de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11 rue Humann, 67085,
Strasbourg, France.; Faculté de Chirurgie Dentaire, Université de Strasbourg, 1
Place de l'Hôpital, 67000, Strasbourg, France.; Institut de Physique et Chimie des
Matériaux de Strasbourg, UMR 7054 CNRS, 23 rue du Loess, Strasbourg Cedex 2,
Strasbourg, 67034, France.; Institut de Physique et Chimie des Matériaux de
Strasbourg, UMR 7054 CNRS, 23 rue du Loess, Strasbourg Cedex 2, Strasbourg, 67034,
France.; Institut Charles Sadron, CNRS UPR 22, Strasbourg, 67034, France.;
Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim,
University of Heidelberg, Theodor-Kutzer Uber 1-3, 68167, Mannheim, Germany.; Red
Cross Blood Service Baden-Württemberg - Hessen, Friedrich-Ebert Str. 107, D-68167,
Mannheim, Germany.; Laboratory for Translational Cellular and Molecular
Biomedicine, Tomsk State University, 36 Lenin Prospekt, Tomsk, 634050, Russia.; Red
Cross Blood Service Baden-Württemberg - Hessen, Friedrich-Ebert Str. 107, D-68167,
Mannheim, Germany.; Institut National de la Santé et de la Recherche Médicale,
INSERM Unité 1121, 11 rue Humann, 67085, Strasbourg, France.; Institut Charles
Sadron, CNRS UPR 22, 23 rue du Lœss, 67034, Strasbourg, France.; Institut National
de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11 rue Humann, 67085,
Strasbourg, France.; Faculté de Chirurgie Dentaire, Université de Strasbourg, 1
Place de l'Hôpital, 67000, Strasbourg, France.
PY - 2015
SP - 2026-36
ST - Harnessing the multifunctionality in nature: a bioactive agent release system
with self-antimicrobial and immunomodulatory properties
T2 - Advanced healthcare materials
TI - Harnessing the multifunctionality in nature: a bioactive agent release system
with self-antimicrobial and immunomodulatory properties
VL - 4
Y2 - 9 y3 - 16
ID - 10145
ER -
TY - JOUR
AB - Quercetin is a plant origin phytochemical with several pharmaceutical
activities such as antioxidant, immunomodulatory, and anti-inflammatory effects.
However, consumption of quercetin is limited due to its low aqueous solubility and
poor bioavailability. The aim of the present study was to synthesize silver and
gold nanoparticles of quercetin with a view to improve its aqueous phase solubility
and investigate the effects on LPS-induced neuroinflammation in BV-2 microglial
cells. The average size of silver and gold-quercetin nanoparticles was 53 and
27 nm, respectively. Absorption peaks in the UV-Vis spectra were observed at 555
and 405 nm for gold and silver-quercetin nanoparticles, respectively. The particle
size and mapping of silver and gold-quercetin nanoparticles were also determined
using a STEM detector. The inflammatory stimulation of the BV-2 cells with LPS
caused an elevated release of proinflammatory prostaglandin, E2, nitric oxide (NO),
upregulated cyclooxygenase-2, inducible NO synthase mRNA, and protein levels, which
were markedly inhibited by the pretreatment with gold-quercetin nanoparticles
(highly soluble in water) without causing any cytotoxic effects. The findings of
the present study suggest that the potential of gold-quercetin nanoparticles are
much better than quercetin and that gold-quercetin nanoparticles might provide
protection against inflammatory neurodegenerative disease via suppression of acute
microglial activation.
AN - rayyan-553781894
AU - Ozdal, Z. D.
AU - Sahmetlioglu, E.
AU - Narin, I.
AU - Cumaoglu, A.
DO - 10.1007/s13205-019-1739-z
IS - 6
J2 - 3 Biotech
LA - eng
N1 - 1Faculty of Pharmacy, Erciyes University, Kayseri, Turkey. ISNI: 0000 0001
2331 2603. GRID: grid.411739.9; 2Nanotechnology Research Center, Erciyes
University, Kayseri, Turkey. ISNI: 0000 0001 2331 2603. GRID: grid.411739.9; Safiye
Çıkrıkçıoğlu Vocational School, Kayseri University, Kayseri, Turkey.; 4Department
of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey.
ISNI: 0000 0001 2331 2603. GRID: grid.411739.9; 5Department of Biochemistry,
Faculty of Pharmacy, Erciyes University, Kayseri, Turkey. ISNI: 0000 0001 2331
2603. GRID: grid.411739.9
PY - 2019
SN - 2190-572X (Print)
SP - 212
ST - Synthesis of gold and silver nanoparticles using flavonoid quercetin and
their effects on lipopolysaccharide induced inflammatory response in microglial
cells
T2 - 3 Biotech
TI - Synthesis of gold and silver nanoparticles using flavonoid quercetin and
their effects on lipopolysaccharide induced inflammatory response in microglial
cells
VL - 9
Y2 - 6
ID - 9817
ER -
TY - JOUR
AB - The effective control of microbial and metabolically derived biological
toxins which negatively impact physical health remains a key challenge for the 21st
century. 2-Dimensional graphene and MXene nanomaterials are relatively new
additions to the field of biomedical materials with superior external surface areas
suited to adsorptive remediation of biological toxins. However, relatively little
is known about their physiological interactions with biological systems and, to
date, no comparative biological studies have been done. This study compares
titanium carbide MXene (Ti3C2Tx) in multilayered and delaminated forms with
graphene variants to assess the impact of variable physical properties on cellular
inflammatory response to endotoxin stimulus. No significant impact on cell
metabolism or induction of inflammatory pathways leading to cell death was
observed. No significant increase in markers of blood cell activation and
haemolysis occurred. Whilst graphene nanoplatelets (GNP), graphene oxide (GO) and
Ti3C2Tx showed insignificant antibacterial activity towards Escherichia coli,
silver nanoparticle-modified GO (GO-Ag) induced bacterial cell death and at a lower
dose than silver nanoparticles. All nanomaterials significantly reduced bacterial
endotoxin induced THP-1 monocyte IL-8, IL-6 and TNF-α cytokine production by >99%,
>99% and >80% respectively, compared to control groups. This study suggests the
utility of these nanomaterials as adsorbents in blood contacting medical device
applications for removal of inflammatory cytokines linked to poor outcome in
patients with life-threatening infection. © The Royal Society of Chemistry.
AN - rayyan-553781157
AU - Ozulumba, T.
AU - Ingavle, G.
AU - Gogotsi, Y.
AU - Sandeman, S.
DO - 10.1039/d0bm01953d
IS - 5
KW - Graphite
Humans
Inflammation
Metal Nanoparticles
Silver
Titanium
Biomedical materials
Blood
Cell death
Escherichia coli
Graphene Nanoplatelets
Metabolism
Metal nanoparticles
Titanium carbide
caspase 3
deionized water
endotoxin
functional group
graphene
graphene oxide
interleukin 6
interleukin 8
mxene
octoxinol
silver nanoparticle
titanium carbide
unclassified drug
graphite
metal nanoparticle
silver
titanium
Bacterial endotoxins
Cytokine production
Life-threatening infections
Physiological interactions
Variable physical properties
adsorption
apoptosis
Article
bacterial cell
cell death
cell interaction
cell metabolism
cell viability
comparative study
controlled study
cytokine production
cytolysis
cytotoxicity
death
DNA fragmentation
fluorescence
hemolysis
hydrodynamics
inflammation
membrane damage
mitochondrial membrane
nonhuman
oxidation
oxidative stress
particle size
pH
priority journal
surface area
surface charge
surface property
THP-1 cell line
thrombocyte activation
ultrasound
human
Graphene
PY - 2021
SP - 1805-1815
ST - Moderating cellular inflammation using 2-dimensional titanium carbide MXene
and graphene variants
T2 - Biomaterials Science
TI - Moderating cellular inflammation using 2-dimensional titanium carbide MXene
and graphene variants
85102476795&doi=10.1039%2fd0bm01953d&partnerID=40&md5=8f0ca4cccbe578ff1fc9924c4b062
482
VL - 9
ID - 9107
ER -
TY - JOUR
AB - Diagnosis of cancer and photothermal therapy using optoelectronic properties
of noble metal nanoparticles (NPs) has established a new therapeutic approach for
treating cancer. Here we address the intrinsic properties of noble metal NPs (gold
and silver) as well as the mechanism of their potential antitumor activity. For
this, the study addresses the functional characterization of tumor associated
macrophages (TAMs) isolated from murine fibrosarcoma induced by a chemical
carcinogen, 3-methylcholanthrene (MCA). We have previously shown antitumor activity
of both gold nanoparticles (AuNPs) and silver nanoparticle (AgNPs) in vivo in a
murine fibrosarcoma model. In the present study, it has been seen that AuNPs and
AgNPs modulate the reactive oxygen species (ROS) and reactive nitrogen species
(RNS) production, suppressing the antioxidant system of cells (TAMs). Moreover, the
antioxidant-mimetic action of these NPs maintain the ROS and RNS levels in TAMs
which act as second messengers to activate the proinflammatory signaling cascades.
Thus, while there is a downregulation of tumor necrosis factor-α (TNF-α) and
Interleukin-10 (IL-10) in the TAMs, the proinflammatory cytokine Interleukin-12
(IL-12) is upregulated resulting in a polarization of TAMs from M2 (anti-
inflammatory) to M1 (pro-inflammatory) nature.
AN - rayyan-553782149
AU - Pal, R.
AU - Chakraborty, B.
AU - Nath, A.
AU - Singh, L. M.
AU - Ali, M.
AU - Rahman, D. S.
AU - Ghosh, S. K.
AU - Basu, A.
AU - Bhattacharya, S.
AU - Baral, R.
AU - Sengupta, M.
DO - 10.1016/j.intimp.2016.06.006
J2 - Int Immunopharmacol
KW - Animals
Cells, Cultured
Fibrosarcoma/chemically induced/*immunology
Gold/chemistry
Macrophages/*physiology
Male
Metal Nanoparticles/*administration & dosage/chemistry
Methylcholanthrene/toxicity
Mice
Oxidative Stress
Phenotype
Silver/chemistry
Macrophages
Metal Nanoparticles
LA - eng
N1 - Department of Biotechnology, Assam University, Silchar, Assam, India,
788011.; Department of Biotechnology, Assam University, Silchar, Assam, India,
788011.; Department of Chemistry, Assam University, Silchar, Assam, India, 788011.;
Department of Chemistry, Assam University, Silchar, Assam, India, 788011.;
Department of Chemistry, Assam University, Silchar, Assam, India, 788011.;
Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute,
Kolkata, India, 700026.; Department of Cancer Chemoprevention, Chittaranjan
National Cancer Institute, Kolkata, India, 700026.; Department of Immunoregulation
and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India,
788011. Electronic address: senguptamahuya35@gmail.com.
PY - 2016
SP - 332-41
ST - Noble metal nanoparticle-induced oxidative stress modulates tumor associated
macrophages (TAMs) from an M2 to M1 phenotype: An in vitro approach
T2 - International immunopharmacology
TI - Noble metal nanoparticle-induced oxidative stress modulates tumor associated
macrophages (TAMs) from an M2 to M1 phenotype: An in vitro approach
VL - 38
Y2 - 9
ID - 10060
ER -
TY - JOUR
AB - The novel coronavirus disease (COVID-19) pandemic has grasped the entire
world due to its high rate of spread with serious public health concern. The
scientific community has applied all possible therapeutic strategies to defeat the
virus, still the situation is not in control. So, as a fresh approach, the
"phytonanoparticles" can be used as a powerful gadget against COVID-19 because it
can be formulated to perform directly concerning the infection, enhancing drug
delivery system or by the way of stimulating the immunity of the patient. The plant
extract bioactive can offer its antioxidant, anti-inflammatory, immunomodulatory
effect for prophylaxis and treatment of SARS-CoV-2. Selective drug targeting of
these plant compounds is needed for augmenting drug stability, solubility,
increasing drug half-lives in the blood and reducing adverse effects in non-target
organs. Green nano-based drugs use plant extract as a bioreduction and capping
agent at room temperature. The green nanoparticles can be aimed to decrease the
oxidative stress and systemic inflammation of COVID-19 with increased activity and
lesser toxicity to normal cells. This review work summarises the antiviral,
immunomodulatory, antiinflammatory potential of green nanoparticles biosynthesised
with plant derived molecules with advanced delivery systems which has a possibility
to act as efficient potential remedy against coronavirus. This review discusses the
scientific explorations of phytonanoparticles which can protect human lives from
the devastation of SARS-CoV-2 because of its enhanced anticoronavirus biological
activity.
AN - rayyan-553781158
AU - Palai, S.
AU - Patra, R.
AU - Dehuri, M.
DO - 10.21276/ap.covid19.2021.10.1.20
IS - 1
PY - 2021
SN - 2393-9885 2278-9839
SP - S222-S230
ST - Phytonanoparticles and COVID-19
T2 - ANNALS OF PHYTOMEDICINE-AN INTERNATIONAL JOURNAL
TI - Phytonanoparticles and COVID-19
VL - 10
Y2 - 1
ID - 9108
ER -
TY - JOUR
AB - OBJECTIVE: Dystrophin, the missing or defective protein in Duchenne muscular
dystrophy, is expressed not only in muscle cells but also in vascular endothelial
cells (ECs). In this study, we assessed the effects of dystrophin deficiency on the
angiogenic capacities of ECs. APPROACH AND RESULTS: We isolated vascular ECs from
mdx mice, the murine equivalent of Duchenne muscular dystrophy in humans, and wild-
type controls, and we found that mdx-derived ECs have impaired angiogenic
properties, in terms of migration, proliferation, and tube formation. They also
undergo increased apoptosis in vitro compared with wild-type cells and have
increased senescence-associated β-galactosidase activity. Mdx-derived ECs also
display reduced ability to support myoblast proliferation when cocultured with
satellite cell-derived primary myoblasts. These endothelial defects are mirrored by
systemic impairment of angiogenesis in vivo, both on induction of ischemia,
stimulation with growth factors in the corneal model and matrigel plug assays, and
tumor growth. We also found that dystrophin forms a complex with endothelial NO
synthase and caveolin-1 in ECs, and that NO production and cGMP formation are
compromised in ECs isolated from mdx mice. Interestingly, treatment with aspirin
enhances production of both cGMP and NO in dystrophic ECs, whereas low-dose aspirin
improves the dystrophic phenotype of mdx mice in vivo, in terms of resistance to
physical exercise, muscle fiber permeability, and capillary density. CONCLUSIONS:
These findings demonstrate that impaired angiogenesis is a novel player and
potential therapeutic target in Duchenne muscular dystrophy.
AN - rayyan-553782223
AU - Palladino, M.
AU - Gatto, I.
AU - Neri, V.
AU - Straino, S.
AU - Smith, R. C.
AU - Silver, M.
AU - Gaetani, E.
AU - Marcantoni, M.
AU - Giarretta, I.
AU - Stigliano, E.
AU - Capogrossi, M.
AU - Hlatky, L.
AU - Landolfi, R.
AU - Pola, R.
DO - 10.1161/ATVBAHA.112.301172
IS - 12
J2 - Arterioscler Thromb Vasc Biol
KW - Animals
Apoptosis
Aspirin/pharmacology
Carcinoma, Lewis Lung/blood supply/metabolism/pathology
Caveolin 1/metabolism
Cell Movement
Cell Proliferation
Cells, Cultured
Cellular Senescence
Corneal Neovascularization/metabolism/pathology/physiopathology
Cyclic GMP/metabolism
Dystrophin/genetics/*metabolism
Endothelial Cells/drug effects/*metabolism/pathology
Endothelium, Vascular/drug effects/*metabolism/pathology/physiopathology
Ischemia/metabolism/pathology/physiopathology
Mice
Mice, Inbred mdx
Muscular Dystrophy, Duchenne/drug
therapy/genetics/*metabolism/pathology/physiopathology
Mutation
Myoblasts, Skeletal/metabolism/pathology
*Neovascularization, Physiologic/drug effects
Nitric Oxide/metabolism
Nitric Oxide Synthase Type III/metabolism
Time Factors
Muscular Dystrophies
Endothelium, Vascular
LA - eng
N1 - From the Division of Cardiovascular Research, Department of Medicine (M.P.,
V.N., R.C.S., M.S., R.P.), and Center of Cancer Systems Biology (R.C.S., L.H.,
R.P.), St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston,
MA; Laboratory of Vascular Biology and Genetics and Department of Medicine, A.
Gemelli University Hospital, Catholic University School of Medicine, Rome, Italy
(M.P., I.G., V.N., E.G., M.M., I.G., E.S., R.L., R.P.); and Laboratory of Vascular
Pathology, IDI Research Institute, Rome, Italy (S.S., M.C.).
PY - 2013
SP - 2867-76
ST - Angiogenic impairment of the vascular endothelium: a novel mechanism and
potential therapeutic target in muscular dystrophy
T2 - Arteriosclerosis, thrombosis, and vascular biology
TI - Angiogenic impairment of the vascular endothelium: a novel mechanism and
potential therapeutic target in muscular dystrophy
VL - 33
Y2 - 12
ID - 10133
ER -
TY - JOUR
AB - As the knowledge about the interferences of nanomaterials on human staminal
cells are scarce and contradictory, we undertook a comparative multidisciplinary
study based on the size effect of zero-valent iron, cobalt, and nickel
microparticles (MPs) and nanoparticles (NPs) using human adipose stem cells (hASCs)
as a model, and evaluating cytotoxicity, morphology, cellular uptake, and gene
expression. Our results suggested that the medium did not influence the cell
sensitivity but, surprisingly, the iron microparticles (FeMPs) resulted in being
toxic. These data were supported by modifications in mRNA expression of some genes
implicated in the inflammatory response. Microscopic analysis confirmed that NPs,
mainly internalized by endocytosis, persist in the vesicles without any apparent
cell damage. Conversely, MPs are not internalized, and the effects on hASCs have to
be ascribed to the release of ions in the culture medium, or to the reduced oxygen
and nutrient exchange efficiency due to the presence of MP agglomerating around the
cells. Notwithstanding the results depicting a heterogeneous scene that does not
allow drawing a general conclusion, this work reiterates the importance of
comparative investigations on MPs, NPs, and corresponding ions, and the need to
continue the thorough verification of NP and MP innocuousness to ensure unaffected
stem cell physiology and differentiation.
AN - rayyan-553781160
AU - Palombella, S.
AU - Pirrone, C.
AU - Rossi, F.
AU - Armenia, I.
AU - Cherubino, M.
AU - Valdatta, L.
AU - Raspanti, M.
AU - Bernardini, G.
AU - Gornati, R.
DO - 10.3390/nano7080212
IS - 8
KW - Metals
PY - 2017
SN - 2079-4991
ST - Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model
T2 - NANOMATERIALS
TI - Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model
VL - 7
Y2 - 8
ID - 9109
ER -
TY - JOUR
AB - Background: The causes of most arthropathies, osteoarthritis, and connective
tissue disorders remain unknown, but exposure to toxic metals could play a part in
their pathogenesis. Human exposure to mercury is common, so to determine whether
mercury could be affecting joints, bones, and connective tissues we used a
histochemical method to determine the cellular uptake of mercury in mice. Whole
neonatal mice were examined since this allowed histological assessment of mercury
in joint, bone, and connective tissue cells. Materials and Methods: Pregnant mice
were exposed to a non-toxic dose of 0.5 mg/m(3) of mercury vapor for 4 h a day on
gestational days 14-18. Neonates were sacrificed at postnatal day 1, fixed in
formalin, and transverse blocks of the body were processed for paraffin embedding.
Seven micrometer sections were stained for inorganic mercury using silver nitrate
autometallography, either alone or combined with CD44 immunostaining to detect
progenitor cells. Control neonates were not exposed to mercury during gestation.
Results: Uptake of mercury was marked in synovial cells, articular chondrocytes,
and periosteal and tracheal cartilage cells. Mercury was seen in fibroblasts in the
dermis, aorta, esophagus and striated muscle, some of which were CD44-positive
progenitor cells, and in the endothelial cells of small blood vessels. Mercury was
also present in renal tubules and liver periportal cells. Conclusions: Mercury is
taken up selectively by cells that are predominantly affected in rheumatoid
arthritis and osteoarthritis. In addition, fibroblasts in several organs often
involved in multisystem connective tissue disorders take up mercury. Mercury
provokes the autoimmune, inflammatory, genetic, and epigenetic changes that have
been described in a range of arthropathies and bone and connective tissue
disorders. These findings support the hypothesis that mercury exposure could
trigger some of these disorders, particularly in people with a genetic
susceptibility to autoimmunity.
AN - rayyan-553782283
AU - Pamphlett, R.
AU - Kum Jew, S.
DO - 10.3389/fmed.2019.00168
J2 - Front Med (Lausanne)
KW - Connective Tissue
LA - eng
N1 - Discipline of Pathology, Brain and Mind Centre, Sydney Medical School, The
University of Sydney, Sydney, NSW, Australia.; Department of Neuropathology, Royal
Prince Alfred Hospital, Sydney, NSW, Australia.; Discipline of Pathology, Brain and
Mind Centre, Sydney Medical School, The University of Sydney, Sydney, NSW,
Australia.
PY - 2019
SN - 2296-858X (Print)
SP - 168
ST - Mercury Is Taken Up Selectively by Cells Involved in Joint, Bone, and
Connective Tissue Disorders
T2 - Frontiers in medicine
TI - Mercury Is Taken Up Selectively by Cells Involved in Joint, Bone, and
Connective Tissue Disorders
VL - 6
ID - 10193
ER -
TY - JOUR
AB - Slow wound healing caused by bacterial infection is a critical clinical
challenge. Moreover, finding common preservatives that fulfill current medical
requirements has proved difficult. Therefore, we designed a multi-functional wound
dressing based on the hydrogen-bonded assembly of polyvinyl alcohol (PVA)/polyhexa-
methylene biguanide (PHMB)/platelet-rich plasma (PRP). The hydrogels were prepared
by a simple and environmentally friendly procedure by repeated freeze-thaw cycles
without a chemical crosslinking agent. Characterization studies revealed the
outstanding stability of these composite hydrogels. Bacteriostatic ring experiments
revealed that the PHMB-containing hydrogel possessed strong antibacterial effects.
The hydrogel with PRP showed low cytotoxicity and significantly stimulated the
proliferation of L929 fibroblasts, according to live/dead cell staining and 3-(4,5-
dimethylthiazol-2-yl)- 5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2 H-
tetrazolium (MTS) testing results. The PVA/PHMB/PRP composite dressing reduced
inflammatory response, promoted epithelization, enhanced collagen production and
vascular regeneration, and restored skin appendages in vivo. These results indicate
that the PVA/PHMB/PRP wound dressing is a promising biological material with
synergistic antibacterial and wound-healing functions.
AN - rayyan-553781161
AU - Pan, L. F.
AU - Li, C. H.
AU - Wang, Z. C.
AU - Yang, L. H.
AU - Zhang, L. B.
DO - 10.1016/j.bej.2022.108626
KW - Bandages
Blood Platelets
Biguanides
PY - 2022
SN - 1369-703X 1873-295X
ST - Preparation of an antibacterial dressing for simultaneous delivery of
polyhexamethylene biguanide and platelet-rich plasma, and evaluation of the
dressing's ability to promote infected skin repair
T2 - BIOCHEMICAL ENGINEERING JOURNAL
TI - Preparation of an antibacterial dressing for simultaneous delivery of
polyhexamethylene biguanide and platelet-rich plasma, and evaluation of the
dressing's ability to promote infected skin repair
VL - 187
Y2 - 11
ID - 9110
ER -
TY - JOUR
AB - Objective: To study the dynamic expression and distribution of high mobility
group box 1 (HMGB-1) in diffuse axonal injury (DAI) in rats and to clarify its
involvement in the inflammatory reaction after DAI in rats, in order to provide new
targets for the clinical treatment of DAI. Methods: A DAI model was established
using a coronal rotation device and evaluated by HE, Glees-Marsland silver
staining, and Mallory phosphotungstic acid hematoxylin staining.
Immunohistochemistry, Western blot and RT-PCR were used to detect the expression
and distribution of HMGB-1 in the cortex of DAI rats at 6 h, 1 d, 3 d and 7 d. And
TUNEL was used to examine the apoptosis of neurons in DAI rats. Results:
Immunohistochemical results showed that at 6 h and 1 d after DAI, the number of
HMGB-1-positive cells decreased, but at 3 and 7 d it began to increase. Western
blot also showed that during the early stage after DAI (6 h and 1 d), the level of
HMGB-1 protein in the cortex was significantly lower than that in the control
group, but at the late stage (3 and 7 d) after DAI it significantly increased
compared with that in the control group until 7 d. RT-PCR showed that at 6 h after
DAI there was no significant increase in the level of HMGB-1mRNA, but at 1 d there
was a slight increase compared with the control group; at 3 and 7 d, it showed an
obvious significance. TUNEL staining indicated that the significant neuronal
apoptosis appeared as early as 6 h after DAI, and reached the peak at 3 d; it
started to decrease at 7 d but still remained at a relatively high level.
Conclusion: The dynamic expression and distribution of HMGB-1 showed significant
changes with the time course after DAI in rats. They decreased at the early stage
but increased at the late stage. At the early stage, HMGB-1 is mainly passively
released by the necrotic neurons, and at the late stage it may be actively secreted
by the active inflammatory cells. HMGB-1 may mediate the post-DAI neural cell
apoptosis by inducing the inflammatory reaction. ©, 2015, Journal of Xi'an Jiaotong
University (Medical Sciences). All right reserved.
AN - rayyan-553781162
AU - Pang, H. G.
AU - Song, J. N.
AU - Li, D. D.
AU - Sun, P.
AU - Zhao, Y. L.
AU - Huang, T. Q.
AU - Zhai, H. C.
AU - An, J. Y.
DO - 10.7652/jdyxb201503004
IS - 3
KW - Apoptosis
Diffuse axonal injury (DAI)
High mobility group box 1
Traumatic brain injury
eosin
hematoxylin
high mobility group protein
messenger RNA
phosphotungstic acid
animal experiment
animal model
animal tissue
apoptosis
Article
axonal injury
brain cortex
control group
controlled study
disease course
nerve cell
nonhuman
protein expression
rat
staining
Western blotting
Rats
Axons
PY - 2015
SP - 304-309
ST - Dynamic expression and distribution of high mobility group box 1 in diffuse
T2 - Journal of Xi'an Jiaotong University (Medical Sciences)
TI - Dynamic expression and distribution of high mobility group box 1 in diffuse
84929618205&doi=10.7652%2fjdyxb201503004&partnerID=40&md5=cf41dbe8670d87a46573ccb3b
5279292
VL - 36
ID - 9111
ER -
TY - JOUR
AB - Bioactive glasses (BGs) have been a focus of research for over five decades
for several biomedical applications. Although their use in bone substitution and
bone tissue regeneration has gained important attention, recent developments have
also seen the expansion of BG applications to the field of soft tissue engineering.
Hard and soft tissue repair therapies can benefit from the biological activity of
metallic ions released from BGs. These metallic ions are incorporated in the BG
network not only for their biological therapeutic effects but also in many cases
for influencing the structure and processability of the glass and to impart extra
functional properties. The "classical" elements in silicate BG compositions are
silicon (Si), phosphorous (P), calcium (Ca), sodium (Na), and potassium (K). In
addition, other well-recognized biologically active ions have been incorporated in
BGs to provide osteogenic, angiogenic, anti-inflammatory, and antibacterial effects
such as zinc (Zn), magnesium (Mg), silver (Ag), strontium (Sr), gallium (Ga),
fluorine (F), iron (Fe), cobalt (Co), boron (B), lithium (Li), titanium (Ti), and
copper (Cu). More recently, rare earth and other elements considered less common
or, some of them, even "exotic" for biomedical applications, have found room as
doping elements in BGs to enhance their biological and physical properties. For
example, barium (Ba), bismuth (Bi), chlorine (Cl), chromium (Cr), dysprosium (Dy),
europium (Eu), gadolinium (Gd), ytterbium (Yb), thulium (Tm), germanium (Ge), gold
(Au), holmium (Ho), iodine (I), lanthanum (La), manganese (Mn), molybdenum (Mo),
nickel (Ni), niobium (Nb), nitrogen (N), palladium (Pd), rubidium (Rb), samarium
(Sm), selenium (Se), tantalum (Ta), tellurium (Te), terbium (Tb), erbium (Er), tin
(Sn), tungsten (W), vanadium (V), yttrium (Y) as well as zirconium (Zr) have been
included in BGs. These ions have been found to be particularly interesting for
enhancing the biological performance of doped BGs in novel compositions for tissue
repair (both hard and soft tissue) and for providing, in some cases, extra
functionalities to the BG, for example fluorescence, luminescence, radiation
shielding, anti-inflammatory, and antibacterial properties. This review summarizes
the influence of incorporating such less-common elements in BGs with focus on
tissue engineering applications, usually exploiting the bioactivity of the BG in
combination with other functional properties imparted by the presence of the added
elements.
AN - rayyan-553782285
AU - Pantulap, U.
AU - Arango-Ospina, M.
AU - Boccaccini, A. R.
DO - 10.1007/s10856-021-06626-3
IS - 1
J2 - J Mater Sci Mater Med
KW - Animals
Biophysical Phenomena/drug effects
Bone Regeneration/drug effects/physiology
Bone Substitutes/chemical synthesis/chemistry/pharmacology
Ceramics/*chemistry/*pharmacology
Coated Materials, Biocompatible/*chemical synthesis/chemistry
Equipment Design/methods/*trends
Humans
Ions
Osteogenesis/drug effects
Eyeglasses
LA - eng
N1 - Department of Materials Science and Engineering, Institute of Biomaterials,
University of Erlangen-Nuremberg, 91058, Erlangen, Germany.; Department of
Materials Science and Engineering, Institute of Biomaterials, University of
Erlangen-Nuremberg, 91058, Erlangen, Germany.; Department of Materials Science and
Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058,
Erlangen, Germany. aldo.boccaccini@ww.uni-erlangen.de.
PY - 2021
SP - 3
ST - Bioactive glasses incorporating less-common ions to improve biological and
physical properties
T2 - Journal of materials science. Materials in medicine
TI - Bioactive glasses incorporating less-common ions to improve biological and
physical properties
VL - 33
Y2 - 12 y3 - 23
ID - 10195
ER -
TY - JOUR
AB - Isohexenylnaphthazarins (IHN), commonly known as Alkannins and Shikonins
(A/S), are lipophilic red pigments. They are found in the outer surface of the
roots of at least a hundred and fifty species that belong to the genera Alkanna,
Lithospermum, Echium, Onosma, Anchusa and Cynoglossum of the Boraginaceae family.
The chiral pairs A/S are potent pharmaceutical substances with a well-established
and wide spectrum of wound healing, antimicrobial, anti-inflammatory, antioxidant,
anticancer and antithrombotic biological activity. For organic chemists uninitiated
in the chemistry of quinones, the structures of alkannin (1) and shikonin (2) may
look misleading simple. However, in spite of great efforts over many years by
several research groups worldwide, a much needed viable synthetic route to these
enantiomers has remained elusive until very recently. The value of A/S motivated
biotechnologists to develop the world's first manufacturing process utilizing plant
cell cultures. The research in this area has provided a wealth of knowledge to the
field of biotechnology. In addition, great insights into the biosynthesis of these
natural products and to our understanding of plant secondary metabolism in general,
has been gained from this work. The last years there has been extensive scientific
research in many areas throughout the disciplines of chemistry and biology and more
specifically in cancer chemotherapy and a number of papers have appeared in the
literature. Significant research has been conducted on A/S effectiveness on several
tumors and on their mechanism of anticancer action. The aim of this paper was to
review the recent advances in chemistry, biology, biotechnology and biosynthesis of
alkannins and shikonins. © 2006 Bentham Science Publishers Ltd.
AN - rayyan-553781163
AU - Papageorgiou, V. P.
AU - Assimopoulou, A. N.
AU - Samanidou, V. F.
AU - Papadoyannis, I. N.
DO - 10.2174/138527206778742704
IS - 16
KW - Alkannins Biosynthesis
Antimicrobial
Antitumor
Bacterial gene ubiC
DNA Topoisomerases
Shikonin derivatives
(3 hydroxyisovaleryl)shikonin
acetylalkannin
acetylshikonin
alkannin
angelylalkannin
angelylshikonin
anhydroalkannin
arnebin 1
arnebin 2
arnebin 3
arnebin 4
arnebin 5
arnebin 6
cycloarnebin 7
deoxyshikonin
fluconazole
haloacetylshikonin derivative
hydrocortisone
isobutylalkannin
isobutylshikonin
meticillin
olive oil
plant extract
povidone iodine
propionylshikonin
shikonin derivative
sulfadiazine silver
teracrylalkannin
unclassified drug
unindexed drug
vancomycin
apoptosis
arthritis
bacterial strain
biological activity
biotechnology
cancer inhibition
drug effect
drug efficacy
drug indication
drug isolation
drug mechanism
drug research
drug structure
enantioselectivity
enzyme inhibition
epithelization
Escherichia coli
human
molecular biology
nonhuman
oligomerization
plant cell culture
polymerization
review
wound healing
Biotechnology
PY - 2006
SP - 2123-2142
ST - Recent advances in chemistry, biology and biotechnology of Alkannins and
Shikonins
T2 - Current Organic Chemistry
TI - Recent advances in chemistry, biology and biotechnology of Alkannins and
Shikonins
33750556630&doi=10.2174%2f138527206778742704&partnerID=40&md5=c69a1bb8c935104885841
fb5eec9f19b
VL - 10
ID - 9112
ER -
TY - JOUR
AB - Tendon injuries are commonly met in the emergency department. Unfortunately,
tendon tissue has limited regeneration potential and usually the consequent
formation of scar tissue causes inferior mechanical properties. Nanoparticles could
be used in different way to improve tendon healing and regeneration, ranging from
scaffolds manufacturing (increasing the strength and endurance or anti-adhesions,
anti-microbial, and anti-inflammatory properties) to gene therapy. This paper aims
to summarize the most relevant studies showing the potential application of
nanoparticles for tendon tissue regeneration. © 2016 Parchi, Vittorio, Andreani,
Battistini, Piolanti, Marchetti, Poggetti and Lisanti.
AN - rayyan-553781164
AU - Parchi, P. D.
AU - Vittorio, O.
AU - Andreani, L.
AU - Battistini, P.
AU - Piolanti, N.
AU - Marchetti, S.
AU - Poggetti, A.
AU - Lisanti, M.
DO - 10.3389/fnagi.2016.00202
KW - Gold nanoparticles
Nanoparticles
Scaffold
Tendon injuries
cellulose
chitosan
dextran
gold nanoparticle
ibuprofen
mesoporous silica nanoparticle
microRNA
nanocrystal
nanoparticle
polyglactin
polylactide
silver nanoparticle
achilles tendon
antiadhesion activity
bacterium adherence
biocompatibility
biotechnological production
cell labeling
cell viability
drug activity
drug release
fiber
human
nanotechnology
nonhuman
nonviral gene delivery system
Short Survey
stem cell
tendon
tendon injury
tissue engineering
tissue regeneration
Tendons
Regeneration
Tendinopathy
PY - 2016
ST - Nanoparticles for tendon healing and regeneration: Literature review
T2 - Frontiers in Aging Neuroscience
TI - Nanoparticles for tendon healing and regeneration: Literature review
84990062648&doi=10.3389%2ffnagi.2016.00202&partnerID=40&md5=476c4a06443ddf704705d64
ee34f5ec0
VL - 8
ID - 9113
ER -
TY - JOUR
AB - Natural bioflavonoids are an essential component of dietary supplements
possessing antimicrobial properties. Many of the bioflavonoids have resulted in
positive antitumor, anticancer, antibacterial, antifungal, anti-inflammatory
properties, but the efficacy remains low due to toxicity at the molecular level
whereas antiviral property limits to negative. The synergistic link between
nanoscience and flavonoid chemistry enhances the epidemiological properties of
flavonoid and also diminish the antimicrobial resistivity (AMR) by forming their
hybrid nanocomposites. Nanochemistry uses various nanocomposite and nano materials
for biosensing the flavonoids and their delivery as a drug. The quercetin flavonoid
and its derivatives such as rutin, and myricetin are used for sensing and drug
delivery. Quercetin with 15Carbon-5Hydroxyl chemical scaffold has been explored for
a few decades for the development of hybrid nanocomposite and nanomaterial with
metallic as well as organic nano co-composites. This quercetin flavonoid based
hybrid nanocomposites seemed to show a significant effect on In vitro and some
animal model processes along with attenuating lipid peroxidation, platelet
aggregation, and capillary permeability actions. This review mainly focused on the
hybrid nanoscience of quercetin bioflavonoid and its application in numerous
biological, material fields with a future perspective. (c) 2020 Published by
Elsevier B.V. on behalf of King Saud University. This is an open access article
under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - rayyan-553781165
AU - Parhi, B.
AU - Bharatiya, D.
AU - Swain, S. K.
DO - 10.1016/j.jsps.2020.10.017
IS - 12
KW - Flavonoids
PY - 2020
SN - 1319-0164 2213-7475
SP - 1719-1732
ST - Application of quercetin flavonoid based hybrid nanocomposites: A review
T2 - SAUDI PHARMACEUTICAL JOURNAL
TI - Application of quercetin flavonoid based hybrid nanocomposites: A review
VL - 28
Y2 - 12
ID - 9114
ER -
TY - JOUR
AB - OBJECTIVE: To evaluate the consequences of plasma from individuals with
obesity on parameters associated with immunosenescence in unrelated healthy
peripheral blood mononuclear cells (PBMC). METHODS: Freshly isolated PBMC were
incubated in media supplemented with 10% of plasma from individuals with obesity or
control subjects for the first 4 hours of 24 to 120 hours of culture. RESULTS:
Plasma from individuals with obesity modulated the phenotype of healthy PBMC,
leading to a higher rate of apoptosis, lower amounts of phospho-γH2AX and -p53, and
mitochondrial dysfunction. After 120 hours, there was a higher secretion of
inflammatory cytokines IL-1β and IL-8. CD8(+) T lymphocytes presented decreased
expression of CD28, which is associated with the immunosenescent phenotype. CD14(+)
macrophages showed increased expression of CD80 and CD206, suggesting a modulation
in the activation of macrophages. CONCLUSIONS: These results demonstrate that
chronic systemic inflammation observed in obesity induces dysfunctional features in
PBMC that are consistent with premature immunosenescence.
AN - rayyan-553782207
AU - Parisi, M. M.
AU - Grun, L. K.
AU - Lavandoski, P.
AU - Alves, L. B.
AU - Bristot, I. J.
AU - Mattiello, R.
AU - Mottin, C. C.
AU - Klamt, F.
AU - Jones, M. H.
AU - Padoin, A. V.
AU - Guma, F. C. R.
AU - Barbé-Tuana, F. M.
DO - 10.1002/oby.21888
IS - 9
KW - Adult
Apoptosis
CD8-Positive T-Lymphocytes/physiology
Culture Media
Female
Humans
*Immunosenescence
Inflammation/*etiology
Leukocytes, Mononuclear/*physiology
Macrophages
Male
Obesity/*blood
Serum
Signal Transduction/*physiology
Inflammation
LA - eng
N1 - Laboratory of Molecular Biology and Bioinformatics, Department of
Biochemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.;
Postgraduate Program of Biological Sciences: Biochemistry, Universidade Federal do
Rio Grande do Sul, Porto Alegre, Brazil.; Laboratory of Molecular Biology and
Bioinformatics, Department of Biochemistry, Universidade Federal do Rio Grande do
Sul, Porto Alegre, Brazil.; Postgraduate Program of Biological Sciences:
Laboratory of Molecular Biology and Bioinformatics, Department of Biochemistry,
Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.; Center of Obesity
and Metabolic Syndrome, Hospital São Lucas, Pontifícia Universidade Católica do Rio
Grande do Sul, Porto Alegre, Brazil.; Postgraduate Program of Biological Sciences:
Laboratory of Cellular Biochemistry, Department of Biochemistry, Universidade
Federal do Rio Grande do Sul, Porto Alegre, Brazil.; Biomedical Research Institute,
Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.;
Center of Obesity and Metabolic Syndrome, Hospital São Lucas, Pontifícia
Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.; Postgraduate
Program of Biological Sciences: Biochemistry, Universidade Federal do Rio Grande do
Sul, Porto Alegre, Brazil.; Laboratory of Cellular Biochemistry, Department of
Biomedical Research Institute, Pontifícia Universidade Católica do Rio Grande do
Sul, Porto Alegre, Brazil.; Center of Obesity and Metabolic Syndrome, Hospital São
Lucas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre,
Brazil.; Laboratory of Biochemistry and Cellular Biology of Lipids, Department of
Biochemistry, ICBS/Universidade Federal do Rio Grande do Sul, Porto Alegre,
Brazil.; Laboratory of Molecular Biology and Bioinformatics, Department of
Postgraduate Program of Biological Sciences: Biochemistry, Universidade Federal do
Rio Grande do Sul, Porto Alegre, Brazil.
PY - 2017
SP - 1523-1531
ST - Immunosenescence Induced by Plasma from Individuals with Obesity Caused Cell
Signaling Dysfunction and Inflammation
TI - Immunosenescence Induced by Plasma from Individuals with Obesity Caused Cell
Signaling Dysfunction and Inflammation
VL - 25
Y2 - 9
ID - 10117
ER -
TY - JOUR
AB - Little attention has been paid to the toxicity of silver amalgam fillings,
which have been used over the centuries in Dentistry. Amalgam particles may
accidentally and/or traumatically be embedded into the submucosal tissue during
placement of a restoration and perpetuate in such area. This article presents a
case of amalgam tattoo and investigates whether it is related to the patient's
repeated episodes of sinusitis. The patient was a 46-year-old woman with a 2 mm
diameter radiopaque lesion in the right oral mucosa detected on a panoramic
radiograph and presented as a black macula clinically. A complete surgical
resection was carried out. The histopathological examination revealed deposits of
dark-brownish pigments lining the submucosal tissue with adjacent lymphocytic
inflammatory infiltrate and multinucleated giant cells phagocyting pigments. There
was a negative staining for both iron and melanin. One year after lesion removal,
the patient reported that the sinusitis crises had ceased after repeated episodes
for years. It may be speculated that the inflammatory process related to amalgam
tattoo seems to lead to a local immune response that causes sinusitis because it
enhances the human leukocyte antigen DR (HLA-DR) tissue expression.
AN - rayyan-553781166
AU - Parizi, J. L. S.
AU - Nai, G. A.
DO - 10.1590/S1678-77572010000100016
IS - 1
KW - Amalgam
Dental restorative material
HLA-DR
Sinusitis
Tattoo
Tattooing
PY - 2010
SP - 100-104
ST - Amalgam tattoo: A cause of sinusitis?
T2 - Journal of Applied Oral Science
TI - Amalgam tattoo: A cause of sinusitis?
77950887646&doi=10.1590%2fS1678-
77572010000100016&partnerID=40&md5=93bbdbacc6d7ab2c8c09334366194090
VL - 18
ID - 9115
ER -
TY - JOUR
AB - Objectives/Hypothesis: To determine the resorption rate and biocompatibility
characteristics of novel cross-linked hydrogel ventilation tubes and varied
formulations of polyester ventilation tubes in a Chinchilla model. Study Design:
Animal Study. Methods: Three cross-linked glycosaminoglycan hydrogel ventilation
tubes fabricated by cross-linking thiol-modified chondroitin sulfate or thiol-
modified carboxymethylated hyaluronic acid, four different polyester ventilation
tubes (poly L-lactide [PLA], 50/50 poly D,L-lactide-co-glycolide [PLGA], and
silver-impregnated versions of PLA and PLGA tubes) were placed into the tympanic
membranes of chinchillas. Commercially available fluoroplastic ventilation tubes
were placed in the contralateral ear of each animal to serve as a control.
Integrity of the tubes was assessed by weekly otoscopy. Biocompatibility was
assessed by auditory brainstem response, by otoscopic and histologic examination of
the tympanic membrane at the tube site. Results: The hydrogel tubes had very short
resorption times that expanded and enlarged the myringotomy site. PLGA and silver-
coated PLGA tubes maintained their integrity in the tympanic membrane for similar
durations of 18.9 ± 6.4 days and 21.0 ± 6.0 days, respectively. The silver-coated
PLGA tubes had lower neutrophil and fibrosis scores than PLGA tubes. PLA tubes
demonstrated equivalent findings to commercially available nonresorbable tubes with
respect to otoscopic findings, auditory brainstem response thresholds, and
histologic inflammatory scores. Conclusions: Resorbable polyester pressure
equalization tubes demonstrate predictable resorption behavior and similar
biocompatibility characteristics when compared with nonresorbable tubes. Silver
modification may confer some stability to PLGA tubes. Hydrogel tubes have very
short resorption times, tend to enlarge the myringotomy site, and show greater
inflammatory changes. Copyright © 2013 The American Laryngological, Rhinological,
and Otological Society, Inc.
AN - rayyan-553781168
AU - Park, A. H.
AU - Hoyt, D.
AU - Britt, D.
AU - Chase, S.
AU - Tansavatdi, K.
AU - Hunter, L.
AU - McGill, L.
AU - Sheng, X.
AU - Skardal, A.
AU - Prestwich, G. D.
DO - 10.1002/lary.23712
IS - 4
KW - chinchilla
hyaluronic acid
polyester
Resorbable ear ventilation tubes
Absorbable Implants
Animals
Chinchilla
Hydrogel
Middle Ear Ventilation
Models, Animal
Polyesters
Tympanic Membrane
chondroitin sulfate
cross linked glycosaminoglycan hydrogel
glycosaminoglycan
polyglactin
polylactide
thiol
unclassified drug
analytical parameters
animal experiment
animal tissue
article
auditory brainstem response threshold
biocompatibility
controlled study
eardrum
fibrosis
histologic inflammatory score
histopathology
medical parameters
neutrophil count
nonhuman
otoscopy
pressure equalization tube
priority journal
resorption rate
sensory system parameters
silver impregnation
tube
Ventilators, Mechanical
PY - 2013
SP - 1043-1048
ST - Cross-linked hydrogel and polyester resorbable ventilation tubes in a
Chinchilla model
T2 - Laryngoscope
TI - Cross-linked hydrogel and polyester resorbable ventilation tubes in a
Chinchilla model
84875459066&doi=10.1002%2flary.23712&partnerID=40&md5=4351b5e9357444641139b16bda0bc
2f3
VL - 123
ID - 9116
ER -
TY - JOUR
AB - In this study, we identified the toxic effects of sheet-type titania (TNS),
which are being developed as a material for UV-blocking glass, comparing with P25,
a benchmark control for titania, in MH-S cells, a mouse alveolar macrophage cell
line. After 24 h exposure, the TNS-exposed cells formed large vacuoles while the
P25-exposed ones did not. The decreased levels of cell viability were similar
between the P25 and TNS groups, but ATP production was clearly lower in cells
exposed to the TNS. P25 decreased the expression of calnexin protein, an
endoplasmic reticulum (ER) membrane marker, and increased the number of cells
generating ROS in a dose dependent manner. Meanwhile, TNS dilated the ER and
mitochondria and increased the secretion of NO and pro-inflammatory cytokines, but
not of ROS. Subsequently, we studied the molecular response following TNS-induced
vacuolization. TNS started to form vacuoles in the cytosol since 20 min after
exposure, and the expression of the mitochondria function-related genes were down-
regulated the most in the cells exposed for 1 h. After 24 h exposure, the number of
apoptotic cells and the relative levels of BAX to Bcl-2 increased. The expression
of SOD1 protein, but not of SOD2, also dose-dependently increased with an increase
in caspase-8 activity. Additionally, the MAPK pathway was significantly activated,
even though the expression of p-EGFR did not change significantly. Furthermore, the
number of apoptotic cells increased rapidly with time and with the inhibition of
vacuole formation. Taken together, we suggest that P25 and TNS may target different
organelles. In addition, TNS, but not P25, induced paraptosis accompanied by
apoptosis in MH-S cells, and the formation of the cytoplasmic vacuoles allowed
delay apoptosis following TNS exposure. (C) 2014 Elsevier Ireland Ltd. All rights
reserved.
AN - rayyan-553781171
AU - Park, E. J.
AU - Lee, S. Y.
AU - Lee, G. H.
AU - Kim, D. W.
AU - Kim, Y.
AU - Cho, M. H.
AU - Kim, J. H.
DO - 10.1016/j.toxlet.2014.07.027
IS - 1
PY - 2014
SN - 0378-4274 1879-3169
SP - 69-79
ST - Sheet-type titania, but not P25, induced paraptosis accompanying apoptosis in
murine alveolar macrophage cells
TI - Sheet-type titania, but not P25, induced paraptosis accompanying apoptosis in
murine alveolar macrophage cells
VL - 230
Y2 - 10 y3 - 1
ID - 9119
ER -
TY - JOUR
AB - Commercialized dressing materials with or without silver have played a
passive role in early-phase wound healing, protecting the skin defects from
infections, absorbing exudate, and preventing dehydration. Chitosan (CTS)-based
sponges have been developed in pure or hybrid forms for accelerating wound healing,
but their wound-healing capabilities have not been extensively compared with widely
used commercial dressing materials, providing limited information in a practical
aspect. In this study, we have developed CTS-silica (CTS-Si) hybrid sponges with
water absorption, flexibility, and mechanical behavior similar to those of CTS
sponges. In vitro and in vivo tests were performed to compare the CTS-Si sponges
with three commercial dressing materials [gauze, polyurethane (PU), and silver-
containing hydrofiber (HF-Ag)] in addition to CTS sponges. Both in vitro and in
vivo tests showed that CTS-Si sponges promoted fibroblast proliferation, leading to
accelerated collagen synthesis, whereas the CTS sponges did not exhibit significant
differences in fibroblast proliferation and collagen synthesis from gauze, PU, and
HF-Ag sponges. In case of CTS-Si, the inflammatory cells were actively recruited to
the wound by the influence of the released silicon ions from CTS-Si sponges, which,
in return, led to an enhanced secretion of growth factors, particularly TGF- during
the early stage. The higher level of TGF- likely improved the proliferation of
fibroblasts, and as a result, collagen synthesis by fibroblasts became remarkably
productive, thereby increasing collagen density at the wound site. Therefore, the
CTS-Si hybrid sponges have considerable potential as a wound-dressing material for
accelerating wound healing. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res
Part B: Appl Biomater, 105B: 1828-1839, 2017.
AN - rayyan-553781174
AU - Park, J. U.
AU - Jung, H. D.
AU - Song, E. H.
AU - Choi, T. H.
AU - Kim, H. E.
AU - Song, J.
AU - Kim, S.
DO - 10.1002/jbm.b.33711
IS - 7
KW - Wound Healing
PY - 2017
SN - 1552-4973 1552-4981
SP - 1828-1839
ST - The accelerating effect of chitosan-silica hybrid dressing materials on the
early phase of wound healing
TI - The accelerating effect of chitosan-silica hybrid dressing materials on the
early phase of wound healing
VL - 105
Y2 - 10
ID - 9122
ER -
TY - JOUR
AB - The biological response to four well-characterized amorphous silica
nanoparticles was investigated in RAW 264.7 macrophages in view of their potential
application as drug carriers to sites of inflammation. All silica nanoparticles-
induced cell membrane damage, reduced metabolic activity, generated ROS and
released various cytokines, but to different extents. Two silica nanoparticles of
34 nm (A and B) with different zetapotentials were more cytotoxic than (aggregated)
11 and 248 nm nanoparticles, while cytokines were mostly induced by the
(aggregated) 11 nm and only one of the 34 nm nanoparticles (34A). The results
indicate that specific silica nanoparticles may have counterproductive effects, for
example when used as carriers of anti-inflammatory drugs. The physicochemical
properties determining the response of nanoparticles vary for different responses,
implying that a screening approach for the safe development of nanoparticles needs
to consider the role of combinations of (dynamic) physicochemical properties and
needs to include multiple toxicity endpoints.
AN - rayyan-553781176
AU - Park, Mvdz
AU - Lynch, I.
AU - Ramirez-Garcia, S.
AU - Dawson, K. A.
AU - de la Fonteyne, L.
AU - Gremmer, E.
AU - Slob, W.
AU - Briede, J. J.
AU - Elsaesser, A.
AU - Howard, C. V.
AU - van Loveren, H.
AU - de Jong, W. H.
DO - 10.1007/s11051-011-0586-6
IS - 12
KW - Macrophages
PY - 2011
SN - 1388-0764 1572-896X
SP - 6775-6787
ST - In vitro evaluation of cytotoxic and inflammatory properties of silica
nanoparticles of different sizes in murine RAW 264.7 macrophages
TI - In vitro evaluation of cytotoxic and inflammatory properties of silica
nanoparticles of different sizes in murine RAW 264.7 macrophages
VL - 13
Y2 - 12
ID - 9124
ER -
TY - JOUR
AB - In the current study, we analyzed the impact of antenatal betamethasone on
macroscopic cerebellar development, Purkinje cell morphology and the expression of
the neuroprotective protein calbindin-D28k. Pregnant rats (Sprague-Dawley) were
randomly divided into two experimental groups: control (CONT) and betamethasone-
treated (BET). At gestational day 20 (G20), BET dams were subcutaneously injected
with a solution of 0.17 mg kg⁻¹ of betamethasone, while CONT animals received a
similar volume of saline. At postnatal days 22 (P22) and P52, BET and CONT
offspring were behaviorally evaluated, and the cerebella were histologically and
immunohistochemically processed. Animals that were prenatally treated with a single
course of betamethasone exhibited long-lasting behavioral changes consistent with
anxiety-like behavior in the open-field test, together with (1) reduced cerebellar
weight and volume, (2) Purkinje cell dendritic atrophy, and (3) an overexpression
of calbindin-D28k. The current results indicate that an experimental single course
of betamethasone in pregnant rats produces long-lasting anxiety-like behaviors,
together with macroscopic and microscopic cerebellar alterations.
AN - rayyan-553782228
AU - Pascual, R.
AU - Valencia, M.
AU - Larrea, S.
AU - Bustamante, C.
IS - 4
J2 - Acta Neurobiol Exp (Wars)
KW - Age Factors
Animals
Animals, Newborn
Anti-Inflammatory Agents/*toxicity
Betamethasone/*toxicity
Calbindin 1/*metabolism
*Cerebellum/drug effects/embryology/growth & development
Developmental Disabilities/*chemically induced
Embryo, Mammalian
Female
Gestational Age
Male
Organ Size/drug effects
Pregnancy
Purkinje Cells/*drug effects/pathology/ultrastructure
Rats
Silver Staining
LA - eng
N1 - Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias,
Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile,
rodrigo.pascual@ucv.cl.
PY - 2014
SP - 415-23
ST - Single course of antenatal betamethasone produces delayed changes in
morphology and calbindin-D28k expression in a rat's cerebellar Purkinje cells
T2 - Acta neurobiologiae experimentalis
TI - Single course of antenatal betamethasone produces delayed changes in
morphology and calbindin-D28k expression in a rat's cerebellar Purkinje cells
VL - 74
ID - 10138
ER -
TY - JOUR
AB - OBJECTIVE: Colchicine is known to reduce inflammation and improve endothelial
cell function and atherosclerosis in obesity, but there is little knowledge of the
specific circulating leukocyte populations that are modulated by colchicine.
METHODS: A secondary analysis of a double-blind randomized controlled trial of
colchicine 0.6 mg or placebo twice daily for 3 months on circulating leukocyte
populations and regulation of the immune secretome in 35 adults with obesity was
performed. RESULTS: Colchicine altered multiple innate immune cell populations,
including dendritic cells and lymphoid progenitor cells, monocytes, and natural
killer cells when compared with placebo. Among all subjects and within the
colchicine group, changes in natural killer cells were significantly positively
associated with reductions in biomarkers of inflammation, including cyclooxygenase
2, pulmonary surfactant-associated protein D, myeloperoxidase, proteinase 3,
interleukin-16, and resistin. Changes in dendritic cells were positively correlated
with changes in serum heart-type fatty acid-binding protein concentrations.
Additionally, colchicine treatment reduced cluster of differentiation (CD) CD4+ T
effector cells and CD8+ T cytotoxic cells. Conversely, colchicine increased CD4+
and CD8+ T central memory cells and activated CD38(High) CD8+ T cells. Changes in
CD4+ T effector cells were associated with changes in serum heart-type fatty acid-
binding protein. CONCLUSIONS: In adults with obesity, colchicine significantly
affects circulating leukocyte populations involved in both innate and adaptive
immune systems along with the associated inflammatory secretome.
AN - rayyan-553782351
AU - Patel, T. P.
AU - Levine, J. A.
AU - Elizondo, D. M.
AU - Arner, B. E.
AU - Jain, A.
AU - Saxena, A.
AU - Lopez-Ocasio, M.
AU - Dagur, P. K.
AU - Famuyiwa, O.
AU - Gupta, S.
AU - Sarrafan-Chaharsoughi, Z.
AU - Biancotto, A.
AU - McCoy, J. P.
AU - Demidowich, A. P.
AU - Yanovski, J. A.
DO - 10.1002/oby.23632
IS - 2
KW - Adult
Humans
*Colchicine/pharmacology/therapeutic use
*Leukocytes, Mononuclear
Obesity/complications
Inflammation/metabolism
Fatty Acid-Binding Proteins/therapeutic use
Humanities
Humanism
Colchicine
LA - eng
N1 - Section on Growth and Obesity, Division of Intramural Research, Eunice
Kennedy Shriver National Institute of Child Health and Human Development, National
Institutes of Health, Bethesda, Maryland, USA.; Section on Growth and Obesity,
Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child
Health and Human Development, National Institutes of Health, Bethesda, Maryland,
USA.; Section on Growth and Obesity, Division of Intramural Research, Eunice
Institutes of Health, Bethesda, Maryland, USA.; Section on Growth and Obesity,
Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child
Health and Human Development, National Institutes of Health, Bethesda, Maryland,
USA.; Section on Growth and Obesity, Division of Intramural Research, Eunice
Institutes of Health, Bethesda, Maryland, USA.; Flow Cytometry Core, National
Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda,
Maryland, USA.; Flow Cytometry Core, National Heart, Lung, and Blood Institute,
National Institutes of Health, Bethesda, Maryland, USA.; Flow Cytometry Core,
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda,
Maryland, USA.; Section on Growth and Obesity, Division of Intramural Research,
Eunice Kennedy Shriver National Institute of Child Health and Human Development,
National Institutes of Health, Bethesda, Maryland, USA.; Section on Growth and
Obesity, Division of Intramural Research, Eunice Kennedy Shriver National Institute
of Child Health and Human Development, National Institutes of Health, Bethesda,
National Institutes of Health, Bethesda, Maryland, USA.; Center for Human
Immunology, National Institute of Allergy and Infectious Diseases, National
Institutes of Health, Bethesda, Maryland, USA.; Flow Cytometry Core, National
Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda,
National Institutes of Health, Bethesda, Maryland, USA.; Johns Hopkins Community
Physicians at Howard County General Hospital, Johns Hopkins Medicine, Columbia,
National Institutes of Health, Bethesda, Maryland, USA.
PY - 2023
SP - 466-478
ST - Immunomodulatory effects of colchicine on peripheral blood mononuclear cell
subpopulations in human obesity: Data from a randomized controlled trial
TI - Immunomodulatory effects of colchicine on peripheral blood mononuclear cell
subpopulations in human obesity: Data from a randomized controlled trial
VL - 31
Y2 - 2
ID - 10259
ER -
TY - JOUR
AB - Green nanotechnology is an ever-evolving field of research and development
due to an environmentally friendly approach. The present study highlights the green
synthesis using Pancratium parvum bulb extract for the biosynthesis of gold
nanoparticles and enlightens its thorough characterization with biomedical
applications. Here, the biogenic gold nanoparticles (AuNPs) were optimized for
maximum and rapid synthesis and characterized by employing several spectroscopic
and imaging techniques. Further, the synthesized particles were used to study anti-
oxidant, anti-inflammatory, chorioallantoic membrane assay (CAM), anti-
acetylcholinesterase enzyme assay, and binding kinetics study, comprising toxicity
study on Vero cell lines. The findings concluded that statistically optimized
parameters exhibited optimum synthesis of AuNPs with promising anti-oxidant, anti-
inflammatory properties, and potential acetylcholinesterase inhibition as a remedy
for Alzheimer's disease. In addition, surface plasmon resonance (SPR) studies
revealed good affinity kinetics of immobilized acetylcholinesterase enzyme on
sensor chip with KD value 1.449 x 10(-6) M. Concurrently, the effect of AuNPs was
evaluated on Vero cell lines, which exhibited the viability of cells at higher
concentration, and CAM assay did not demonstrate angiogenicity. Thus, this said
approach is rapid with potential medical applications.
AN - rayyan-553781177
AU - Patil, D. N.
AU - Patil, P. J.
AU - Rane, M. R.
AU - Yadav, S. R.
AU - Bapat, V. A.
AU - Vyavahare, G. D.
AU - Jadhav, J. P.
DO - 10.1007/s40089-021-00335-z
IS - 3
PY - 2021
SN - 2008-9295 2228-5326
SP - 215-232
ST - Response surface methodology-based optimization of Pancratium parvum Dalzell-
mediated synthesis of gold nanoparticles with potential biomedical applications
T2 - INTERNATIONAL NANO LETTERS
TI - Response surface methodology-based optimization of Pancratium parvum Dalzell-
mediated synthesis of gold nanoparticles with potential biomedical applications
VL - 11
Y2 - 9
ID - 9125
ER -
TY - JOUR
AB - Prolonged inflammation and infection are the major factors that promote
chronicity of the wound. Despite the substantial advancements in therapeutic
modalities, the treatment of chronic wounds still represents a major clinical
challenge. Conventional remedies are associated with several complications like
drug resistance, sys-temic toxicity, and serious side effects that need to be
addressed. This necessitates the development of safe and alternative pro-healing
agents for efficient wound repair. In this scenario, the application of
phytoconstituents has garnered much attention due to their safety profile and cost-
effectiveness. They enhance the wound repair process by modulating various
signaling pathways like TGF-beta, NF kappa B, Nrf2, MAPK, etc, and promote cell
pro-liferation, migration, differentiation, angiogenesis, and inhibit inflammation.
The topical route of drug admin-istration is the most preferable method of drug
delivery for the effective management of chronic wounds. Currently, much emphasis
has been given to the formulation of different phytoconstituents-based topical
delivery systems like hydrogels, films, foam, sponges, fibers, nanoformulations,
etc. These topical formulations improve the pharmacokinetic and physicochemical
features of phytoconstituents. The main emphasis of the present re-view is to
discuss the therapeutic potential, pharmacological importance, and current clinical
status of various phytoconstituents-based topical formulations as well as their
associated molecular mechanisms in chronic wound management.
AN - rayyan-553781178
AU - Pattnaik, S.
AU - Mohanty, S.
AU - Sahoo, S. K.
AU - Mohanty, C.
DO - 10.1016/j.jddst.2023.104546
PY - 2023
SN - 1773-2247 2588-8943
ST - A mechanistic perspective on the role of phytoconstituents-based
pharmacotherapeutics and their topical formulations in chronic wound management
T2 - JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
TI - A mechanistic perspective on the role of phytoconstituents-based
pharmacotherapeutics and their topical formulations in chronic wound management
VL - 84
Y2 - 6
ID - 9126
ER -
TY - JOUR
AB - Aim: Zinc oxide nanoparticles can be multidimensionally used for treatment,
targeting and diagnosis of lung, breast, skin and many other cancer or tumors.
Coriander oleoresin is derived from dried seeds of Coriandrum sativum. Common uses
of coriander include its application as a diuretic, an antidepressant and a
memoryenhancing compound. Also, it has been shown to exhibit great antioxidative
capacity due to its polyphenolic compounds and further antidiabetic and
antimutagenic effects. The aim of the present study was to evaluate the
antibacterial activity and cytotoxic activity of coriander oleoresin mediated ZnO
nanoparticles. Materials and methods: In the present investigation, coriander
oleoresin mediated synthesis of ZnO nanoparticles was carried out initially and
then confirmed by UV-Visible spectroscopy. The antibacterial activity of the
synthesis ZnO nanoparticles was carried out using agar well diffusion method.
Different concentrations of zinc nanoparticles were tested against Streptococcus
mutans, lactobacillus and Candida albicans. Results: The zinc nanoparticles
prepared from coriander oleoresin showed good anti-inflammatory activity. The zinc
nanoparticles were analysed using UV- Visible spectroscopy and were found to be at
an absorbance range of 300 to 700nm. The peak was found to be a maximum of 350 nm.
The synthesised nanoparticles showed maximum anti-bacterial activity with 9 mm at
150 μL concentration. The study did not exhibit any cytotoxic activity. Conclusion:
In the present study, it was observed that coriander oleoresin mediated zinc
nanoparticles had antibacterial property and also did not exhibit any cytotoxic
activity. Green synthesis of Zinc nanoparticles using coriander oleoresin was of
low cost and was convenient to carry out. Hence, coriander oleoresin mediated zinc
nanoparticles may be used for the control of oral pathogens in large scales. ©
2020, Advanced Scientific Research. All rights reserved.
AN - rayyan-553781180
AU - Paul, R.
AU - Roy, A.
AU - Thangavelu, L.
DO - 10.31838/ijpr/2020.SP1.411
KW - Green synthesis
Nanoparticles
Oleoresin
Oral pathogens
ZnO nanoparticles
aciclovir
amoxicillin
antidepressant agent
antidiabetic agent
antioxidant
diuretic agent
nanoparticle
plant extract
polyphenol derivative
silver nanoparticle
zinc
zinc nanoparticle
agar diffusion
agar dilution
antifungal activity
antimutagenic activity
Article
Bacillus subtilis
bacteriophage
breast
Candida albicans
cell viability
controlled study
coriander
cytotoxicity
drug synthesis
Escherichia coli
infectious agent
Lactobacillus
LC50
lung
malignant neoplasm
MTT assay
nonhuman
phytochemistry
shrimp
skin
Streptococcus mutans
zone of inhibition
Zinc
PY - 2020
SP - 3057-3062
ST - Cytotoxic effect and antibacterial activity of coriander oleoresin mediated
zinc oxide nanoparticles
T2 - International Journal of Pharmaceutical Research
TI - Cytotoxic effect and antibacterial activity of coriander oleoresin mediated
zinc oxide nanoparticles
85104899497&doi=10.31838%2fijpr
%2f2020.SP1.411&partnerID=40&md5=7a24a446c92485d13cc1289ac3538367
VL - 12
ID - 9128
ER -
TY - JOUR
AB - Although a variety of nanoparticles (NPs) functionalized with amphotericin B,
an antifungal agent widely used in the clinic, have been studied in the last years
their cytotoxicity profile remains elusive. Here we show that human endothelial
cells take up high amounts of silica nanoparticles (SNPs) conjugated with
amphotericin B (AmB) (SNP-AmB) (65.4 +/- 12.4 pg of Si per cell) through
macropinocytosis while human fibroblasts internalize relatively low amounts (2.3
+/- 0.4 pg of Si per cell) because of their low capacity for macropinocytosis. We
further show that concentrations of SNP-AmB and SNP up to 400 mu g/mL do not
substantially affect fibroblasts. In contrast, endothelial cells are sensitive to
low concentrations of NPs (above 10 mu g/mL), in particular to SNP-AmB. This is
because of their capacity to internalize high concentration of NPs and high
sensitivity of their membrane to the effects of AmB. Low-moderate concentrations of
SNP-AmB (up to 100 mu g/mL) induce the production of reactive oxygen species (ROS),
LDH release, high expression of pro-inflammatory cytokines and chemokines (IL-8,
IL-6, G-CSF, CCL4, IL-1 beta and CSF2) and high expression of heat shock proteins
(HSPs) at gene and protein levels. High concentrations of SNP-AmB (above 100 mu
g/mL) disturb membrane integrity and kill rapidly human cells (60% after 5 h). This
effect is higher in SNP-AmB than in SNP. (C) 2013 Elsevier Ltd. All rights
reserved.
AN - rayyan-553781181
AU - Paulo, C. S. O.
AU - Lino, M. M.
AU - Matos, A. A.
AU - Ferreira, L. S.
IS - 21
KW - Humanities
Humanism
Humans
Heat-Shock Proteins
Amphotericin B
PY - 2013
SN - 0142-9612 1878-5905
SP - 5281-5293
ST - Differential internalization of amphotericin B - Conjugated nanoparticles in
human cells and the expression of heat shock protein 70
T2 - BIOMATERIALS
TI - Differential internalization of amphotericin B - Conjugated nanoparticles in
human cells and the expression of heat shock protein 70
VL - 34
Y2 - 7
ID - 9129
ER -
TY - JOUR
AB - Alongside physiochemical properties (PCP), it has been suggested that the
protein corona of nanoparticles (NPs) plays a crucial role in the response of
immune cells to NPs. However, due to the great variety of NPs, target cells, and
exposure protocols, there is still no clear relationship between PCP, protein
corona composition, and the immunotoxicity of NPs. In this study, we correlated PCP
and the protein corona composition of NPs to the THP-1 macrophage response,
focusing on selected toxicological endpoints: cell viability, reactive oxygen
species (ROS), and cytokine secretion. We analyzed seven commonly used engineered
NPs (SiO2, silver, and TiO2) and magnetic NPs. We show that with the exception of
silver NPs, all of the tested TiO2 types and SiO2 exhibited moderate toxicities and
a transient inflammatory response that was observed as an increase in ROS, IL-8,
and/or IL-1 beta cytokine secretion. We observed a strong correlation between the
size of the NPs in media and IL-1 beta secretion. The induction of IL-1 beta
secretion was completely blunted in NLR family pyrin domain containing 3 (NLRP3)
knockout THP-1 cells, indicating activation of the inflammasome. The correlations
analysis also implicated the association of specific NP corona proteins with the
induction of cytokine secretion. This study provides new insights toward a better
understanding of the relationships between PCP, protein corona, and the
inflammatory response of macrophages for different engineered NPs, to which we are
exposed on a daily basis.
AN - rayyan-553781182
AU - Pavlin, M.
AU - Lojk, J.
AU - Strojan, K.
AU - Hafner-Bratkovic, I.
AU - Jerala, R.
AU - Leonardi, A.
AU - Krizaj, I.
AU - Drnovsek, N.
AU - Novak, S.
AU - Veranic, P.
AU - Bregar, V. B.
DO - 10.3390/ijms23116197
IS - 11
KW - Macrophages
PY - 2022
SN - 1422-0067
ST - The Relevance of Physico-Chemical Properties and Protein Corona for
Evaluation of Nanoparticles Immunotoxicity-In Vitro Correlation Analysis on THP-1
Macrophages
TI - The Relevance of Physico-Chemical Properties and Protein Corona for
Macrophages
VL - 23
Y2 - 6
ID - 9130
ER -
TY - JOUR
AB - Alongside physiochemical properties (PCP), it has been suggested that the
protein corona of nanoparticles (NPs) plays a crucial role in the response of
immune cells to NPs. However, due to the great variety of NPs, target cells, and
exposure protocols, there is still no clear relationship between PCP, protein
corona composition, and the immunotoxicity of NPs. In this study, we correlated PCP
and the protein corona composition of NPs to the THP-1 macrophage response,
focusing on selected toxicological endpoints: cell viability, reactive oxygen
species (ROS), and cytokine secretion. We analyzed seven commonly used engineered
NPs (SiO(2), silver, and TiO(2)) and magnetic NPs. We show that with the exception
of silver NPs, all of the tested TiO(2) types and SiO(2) exhibited moderate
toxicities and a transient inflammatory response that was observed as an increase
in ROS, IL-8, and/or IL-1β cytokine secretion. We observed a strong correlation
between the size of the NPs in media and IL-1β secretion. The induction of IL-1β
secretion was completely blunted in NLR family pyrin domain containing 3 (NLRP3)
knockout THP-1 cells, indicating activation of the inflammasome. The correlations
analysis also implicated the association of specific NP corona proteins with the
induction of cytokine secretion. This study provides new insights toward a better
understanding of the relationships between PCP, protein corona, and the
inflammatory response of macrophages for different engineered NPs, to which we are
exposed on a daily basis.
AN - rayyan-553782116
AU - Pavlin, M.
AU - Lojk, J.
AU - Strojan, K.
AU - Hafner-Bratkovič, I.
AU - Jerala, R.
AU - Leonardi, A.
AU - Križaj, I.
AU - Drnovšek, N.
AU - Novak, S.
AU - Veranič, P.
AU - Bregar, V. B.
DO - 10.3390/ijms23116197
IS - 11
J2 - Int J Mol Sci
KW - Inflammasomes/metabolism
Macrophages/metabolism
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
*Nanoparticles/chemistry/toxicity
*Protein Corona/metabolism
Silicon Dioxide/metabolism/toxicity
Silver/metabolism/toxicity
Macrophages
LA - eng
N1 - Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov
trg 2, SI-1000 Ljubljana, Slovenia.; Group for Nano and Biotechnological
Application, Faculty of Electrical Engineering, University of Ljubljana, Tržaška
25, SI-1000 Ljubljana, Slovenia.; Group for Nano and Biotechnological Application,
Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000
Ljubljana, Slovenia.; Group for Nano and Biotechnological Application, Faculty of
Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000 Ljubljana,
Slovenia.; Department of Synthetic Biology and Immunology, National Institute of
Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.; EN-FIST Centre of
Excellence, Trg Osvobodilne fronte 13, SI-1000 Ljubljana, Slovenia.; Department of
Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19,
SI-1000 Ljubljana, Slovenia.; EN-FIST Centre of Excellence, Trg Osvobodilne fronte
13, SI-1000 Ljubljana, Slovenia.; Department of Molecular and Biomedical Sciences,
Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.; Department of
Molecular and Biomedical Sciences, Jožef Stefan Institute, Jamova 39, SI-1000
Ljubljana, Slovenia.; Department for Nanostructured Materials, Jožef Stefan
Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.; Department for Nanostructured
Materials, Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.;
Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Vrazov trg
2, SI-1000 Ljubljana, Slovenia.; Group for Nano and Biotechnological Application,
Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000
Ljubljana, Slovenia.
PY - 2022
ST - The Relevance of Physico-Chemical Properties and Protein Corona for
Macrophages
TI - The Relevance of Physico-Chemical Properties and Protein Corona for
Macrophages
VL - 23
Y2 - 5 y3 - 31
ID - 10027
ER -
TY - JOUR
AB - The skin represents our first defense against physical, mechanical, or
biological damage. However, its regeneration potential depends on the degree of the
wound and the systemic state. For large acute or chronic wounds, specialized
treatments are required to stimulate regeneration and avoid scarring. The use of
nanotechnology represents a promising tool to promote wound healing, not only by
developing antibacterial nanomaterials to prevent infection, but also by developing
nanomaterials capable of enhancing cell proliferation, extracellular matrix
deposition, and angiogenesis, as well as regulating inflammation. Nanotechnology
offers a new and wide field of application for materials, due to the unique
properties of nanomaterials that normally differ from, or are enhanced compared to,
bulk materials. Nanomaterial properties changed mainly due to its larger contact
area, which normally results in better optical, catalytic, and biodistribution
properties. Due to their physical–chemical characteristics, nanoparticles can be
tailored in different ways to be delivered and act at the site of injury, improving
wound healing. Nanoparticles can be incorporated into different substrates to
generate antibacterial coverages, they can be functionalized and tailored to be
used as vectors for delivering signaling molecules (growth factors), or their
properties such as size, crystallinity, surface chemistry, and shape can be
modified to elicit different biological effects. The present work is aimed at
resuming the advances of the last decade in nanoparticles used to improve wound
healing based on their antimicrobial activity, cell proliferation enhancement,
angiogenesis promotion, regulation of extracellular matrix deposition, and anti-
inflammatory modulation. Graphical abstract: [Figure not available: see fulltext.].
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.
AN - rayyan-553781185
AU - Pérez-Díaz, M. A.
AU - Prado-Prone, G.
AU - Díaz-Ballesteros, A.
AU - González-Torres, M.
AU - Silva-Bermudez, P.
AU - Sánchez-Sánchez, R.
DO - 10.1007/s11051-023-05675-9
IS - 2
KW - Antibacterial
Cell proliferation
Inflammation
Nanobiomedicine
Nanoparticles
Wound healing
Crystallinity
Deposition
Pathology
Surface chemistry
Tissue regeneration
alginic acid
carbomer
chitosan
cycline
cytokine
dimethyloxalylglycine
gelatin
hexagonal boron nitride
hyaluronic acid
hydrogel
interleukin 1
metal nanoparticle
nanocomposite
nanofiber
nanomaterial
nanoparticle
Pluronic 127
polycaprolactone
polylactic acid nanoparticles
polymyxin
pyridoxine
sericin
serpin A1
silica nanoparticle
silver nanoparticle
sonic hedgehog protein
titanium oxide nanoparticle
tumor necrosis factor alpha receptor
unclassified drug
vasculotropin receptor
Angiogenesis
Antibacterials
Chronic wounds
Extracellular matrix deposition
Mechanical
Property
Wound healing process
3T3 cell line
angiogenesis
angiogenesis improvement
antiinflammatory modulation
apoptosis
bacterial infection control
biodistribution
blood vessel density
cell proliferation enhancement
cell infiltration
cell proliferation
cell viability
chorioallantoic membrane assay
colony forming unit
conductive property
cytotoxicity
Enterobacter cloacae
epithelization
Escherichia coli
extracellular matrix deposition
fibroblast
fibroblast 3T3 cell line
granulation tissue
HaCat cell line
human
immune response
keratinization
keratinocyte
mammal cell
nanobiomedicine
nanotechnology
neonatal human dermal fibroblast
NIH 3T3 cell line
nonhuman
Review
tissue integrity
tubular structure
wound closure
wound healing
zone of inhibition
Wound Healing
PY - 2023
ST - Nanoparticle and nanomaterial involvement during the wound healing process:
an update in the field
TI - Nanoparticle and nanomaterial involvement during the wound healing process:
an update in the field
85146876433&doi=10.1007%2fs11051-023-05675-
9&partnerID=40&md5=c21a083cfb64c54db2fbfe3749df4b69
VL - 25
ID - 9133
ER -
TY - JOUR
AB - The purpose of this study was to compare the intraosseous biocompatibility of
Dyract, a new hydrophilic glass-ionomer cement, to that of Super EBA. Twenty-four
New Zealand rabbits were anesthetized, one leg was shaved, the femur exposed, and
two holes were drilled through the cortical plate, The materials were loaded into
silicone carriers and inserted into the femur. Half of the rabbits were killed 4
weeks after implantation and the other half at 12 weeks and the femurs were
prepared using standard histological procedures, The tissue reactions were graded
from none to severe, At 4 weeks both materials showed slight to moderate reactions,
characterized by the presence of fibrous tissue interposition and inflammatory
cells. At 12 weeks, bone healing had occurred, despite the persistence of some
fibrous tissue interposition, and the reactions were classified as slight. At both
observation periods, statistical analysis failed to show any difference between the
two materials indicating that Dyract and Super EBA had similar intraosseous
biocompatibility.
AN - rayyan-553781186
AU - Pertot, W. J.
AU - Stephan, G.
AU - Tardieu, C.
AU - Proust, J. P.
DO - 10.1016/S0099-2399(97)80413-X
IS - 5
PY - 1997
SN - 0099-2399 1878-3554
SP - 315-319
ST - Comparison of the intraosseous biocompatibility of dyract and super EBA
T2 - JOURNAL OF ENDODONTICS
TI - Comparison of the intraosseous biocompatibility of dyract and super EBA
VL - 23
Y2 - 5
ID - 9134
ER -
TY - JOUR
AB - The surface topographies of nanoporous anodic aluminum oxide (AAO) and
titanium dioxide (TiO2) membranes have been shown to modulate cell response in
orthopedic and skin wound repair applications. In this study, we: (1) demonstrate
an improved atomic layer deposition (ALD) method for coating the porous structures
of 20, 100, and 200 nm pore diameter AAO with nanometer-thick layers of TiO2 and
(2) evaluate the effects of uncoated AAO and TiO2-coated AAO on cellular responses.
The TiO2 coatings were deposited on the AAO membranes without compromising the
openings of the nanoscale pores. The 20 nm TiO2-coated membranes showed the highest
amount of initial protein adsorption via the micro bicinchoninic acid (micro-BOA)
assay; all of the TiO2-coated membranes showed slightly higher protein adsorption
than the uncoated control materials. Cell viability, proliferation, and
inflammatory responses on the TiO2-coated AAO membranes showed no adverse outcomes.
For all of the tested surfaces, normal increases in proliferation (DNA content) of
L929 fibroblasts were observed over from 4 hours to 72 hours. No increases in TNF-
alpha production were seen in RAW 264.7 macrophages grown on TiO2-coated AAO
membranes compared to uncoated AAO membranes and tissue culture polystyrene (TOPS)
surfaces. Both uncoated AAO membranes and TiO2-coated AAO membranes showed no
significant effects on cell growth and inflammatory responses. The results suggest
that TiO2-coated AAO may serve as a reasonable prototype material for the
development of nanostructured wound repair devices and orthopedic implants.
AN - rayyan-553781187
AU - Petrochenko, P. E.
AU - Kumar, G.
AU - Fu, W. J.
AU - Zhang, Q.
AU - Zheng, J. W.
AU - Liang, C. D.
AU - Goering, P. L.
AU - Narayan, R. J.
DO - 10.1166/jbn.2015.2169
IS - 12
KW - Titanium
Aluminum Oxide
PY - 2015
SN - 1550-7033 1550-7041
SP - 2275-2285
ST - Nanoporous Aluminum Oxide Membranes Coated with Atomic Layer Deposition-Grown
Titanium Dioxide for Biomedical Applications: An In Vitro Evaluation
TI - Nanoporous Aluminum Oxide Membranes Coated with Atomic Layer Deposition-Grown
Titanium Dioxide for Biomedical Applications: An In Vitro Evaluation
VL - 11
Y2 - 12
ID - 9135
ER -
TY - JOUR
AB - The chronic toxicity test has been conducted for twenty-eight days to
characterize the hepatic expression levels of eight stress-related genes after
exposing Medaka to two doses of silver nitrate or a silver nanoparticle (Ag-NP)
using real time RT-PCR analysis. This extends our previously published work to
include three additional biomarkers and three later time points. In comparing with
the control, the significant induction of MT and GST genes in livers of the fish
exposed to 1μg/l Ag-NPs was observed at various time points during the test period.
The Orla C3-1 (Medaka) gene was slightly induced only with 1μg/l Ag-NPs at 7-day
exposure while the suppression of p53 and HSP70 was recorded in all exposures at
the end of the test. The gene encoding transferrin was repressed at day 21 by both
silver types and at every exposure dosage. These results revealed that the Ag-NPs
increase metal detoxification, oxidative and inflammatory stress, and finally
stimulate immune responses in Medaka. The conspicuous induction of choriogenin L
and vitellogenin 1 in male fish exposed to Ag-NPs, especially at 7- and 21-day,
compared with the exposures of AgNO 3 or control was the first attempt to examine
estrogenic effects of Ag-NPs. © 2011 Elsevier Inc.
AN - rayyan-553781188
AU - Pham, C. H.
AU - Yi, J.
AU - Gu, M. B.
DO - 10.1016/j.ecoenv.2011.11.034
KW - Biomarker
Chronic toxicity
Medaka
Nanotoxicity
Real-time PCR
Silver nanoparticle
Animals
Biological Markers
Estrogens
Gene Expression
Liver
Male
Metal Nanoparticles
Oryzias
Reverse Transcriptase Polymerase Chain Reaction
Silver
Vitellogenins
Water Pollutants, Chemical
Oryzias latipes
Oryziinae
protein p53
silver nanoparticle
silver nitrate
transferrin
unclassified drug
vitellogenin
vitellogenin 1
biomarker
estrogenic compound
fish
gene
nitrate
silver
toxicity test
animal cell
article
biomarker gene
chronic toxicity
controlled study
cytotoxicity test
detoxification
gene expression
gene location
gene repression
GST gene
immune response
immunostimulation
inflammation
liver
male
marker gene
mt gene
nonhuman
nucleotide sequence
Orla C3 1 gene
oxidative stress
PY - 2012
SP - 239-245
ST - Biomarker gene response in male Medaka (Oryzias latipes) chronically exposed
to silver nanoparticle
TI - Biomarker gene response in male Medaka (Oryzias latipes) chronically exposed
to silver nanoparticle
84857444151&doi=10.1016%2fj.ecoenv.2011.11.034&partnerID=40&md5=cd099d9e933dd775848
6103675a0b625
VL - 78
ID - 9136
ER -
TY - JOUR
AB - Plant secondary metabolites such as flavonoids demonstrate high degrees of
antioxidant, anti-inflammatory, and anticancer activities. Among flavonoids,
quercetin plays an important role in inflammation by downregulating the level of
various cytokines. Thereby, in this work, onion (Allium cepa) peel was successfully
utilized for the synthesis of gold nano-bioconjugates acting as a natural
therapeutic drug. In this process, crude onion peel extract was first divided into
different fractionates, namely, ethyl acetate, butanol, methanol, and water, and
they were subjected to various preliminary studies of antioxidant activities. The
ethyl acetate fractionate shows high antioxidant activities in all the assays. The
bioactive components were identified and found to contain a high amount of
quercetin as confirmed by liquid chromatography with tandem mass spectrometry and
high-performance liquid chromatogrpahy. Three gold nano-bioconjugates were prepared
with different concentrations of the ethyl acetate fractionate. Various biochemical
anti-inflammatory assays were carried out and compared with the active ethyl
acetate fraction of the onion peel drug (OPD). The cytotoxicity of the nano-
bioconjugate system and the OPD was checked in the myoblast L6 cell line from
skeletal muscle tissues to evaluate the toxicity. All the three nano-bioconjugates
A, B, and E demonstrated high percentages of cell viability, viz., 73.07, 72.3, and
69.15%, respectively, at their highest concentration of 200 mu g/mL. The OPD also
showed 88.56% cell viability with no toxic effects in the myoblast L6 cell line
from skeletal muscle tissues. The reactive oxygen species reduction of
nanobioconjugate B showed a marked reduction of 76.77% at a maximum concentration
of 200 mu g/mL, whereas the OPD showed 68.17%. Hence, through this work, a cheap
source of nano-bioconjugates is developed, which can act as a potent antioxidant
and anti-inflammatory agent and are more active in comparison to the OPD alone.
AN - rayyan-553781189
AU - Phukan, K.
AU - Devi, R.
AU - Chowdhury, D.
DO - 10.1021/acsomega.1c00861
IS - 28
KW - Antioxidants
PY - 2021
SN - 2470-1343
SP - 17811-17823
ST - Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and
Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
T2 - ACS OMEGA
TI - Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and
Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
VL - 6
Y2 - 7 y3 - 20
ID - 9137
ER -
TY - JOUR
AB - Two gold(I) complexes featuring 1,2,4-triazole derived carbenes ([AuCl(Cy-
tazy)] and [AuCl(Bn-tazy)]) have been successfully synthesized via a silver-carbene
transfer pathway. Subsequent metal oxidations of using PhICl2 resulted in gold(III)
complexes [AuCl3(Cy-tazy)] and [AuCl3(Bn-tazy)], respectively. All the complexes
have been characterized by elemental analysis, multinuclear NMR spectroscopy, (ESI)
mass spectrometry, single crystal X-ray diffraction and DFT calculations. In
addition, all the four gold complexes exhibit high antiproliferative activities
against KB, MCF-7 and HT-29 cancerous cell lines. The most active compound,
[AuCl(Bn-tazy)], is also potent to Hela and leukemia (HL-60) cells but shows lower
degree of cytotoxicity toward non-cancerous human embryonic kidney cells (HEK-293).
Our preliminary studies also reveal that the two gold(III) complexes are capable of
stabilizing red blood cell membrane and suppressing the NO production in RAW264.7
macrophages induced by lipopolysaccharides, suggesting their potential anti-
inflammatory activities. Interestingly, there have hardly been any reports on anti-
inflammatory activities of gold(III) complexes of N-heterocyclic carbenes. © 2023
Elsevier B.V.
AN - rayyan-553781190
AU - Phung, H. T. T.
AU - Vu, H. M.
AU - Ly, M. Q. H.
AU - Nguyen, H. H.
AU - Nguyen, T. H.
AU - Luong, H. T. T.
AU - Nguyen, V. H.
DO - 10.1016/j.inoche.2023.110898
KW - Triazoles
PY - 2023
ST - Gold(I) and gold(III) complexes of 1,2,4-triazole-derived N-heterocyclic
carbenes: Synthesis, characterization, in-vitro anticancer and anti-inflammatory
studies
T2 - Inorganic Chemistry Communications
TI - Gold(I) and gold(III) complexes of 1,2,4-triazole-derived N-heterocyclic
carbenes: Synthesis, characterization, in-vitro anticancer and anti-inflammatory
studies
85161650795&doi=10.1016%2fj.inoche.2023.110898&partnerID=40&md5=99ad38014423b9e0605
0b82019272067
VL - 154
ID - 9138
ER -
TY - JOUR
AB - Objective. To evaluate the tissue responses to implants of Ketac Silver and
Super EBA cement in the guinea pig mandible. Study design. Sixteen guinea pigs were
used for 2 experimental periods of 4 and 12 weeks. Both materials were placed in
Teflon applicators and implanted into surgically prepared sites in the mandible. A
histologic examination for reaction to the material occurred after the animals were
killed and processed. Results. After 4 weeks, minimal inflammatory reactions were
observed in Ketac Silver implants, whereas the Super EBA implants showed minimal to
moderate inflammation. Localized foreign body reaction was present in areas of
fragmented small particles of Ketac Silver. At 12-weeks observation, no
inflammatory reactions were present around either material. Direct bone contact was
observed in 1 Ketac Silver implant. Conclusions. Ketac Silver and Super EBA cement
elicited mild reactions under the conditions of this model. From a biologic point
of view, these 2 materials may offer equal utility in endodontic surgery.
AN - rayyan-553781196
AU - Pissiotis, E.
AU - Spangberg, L.
DO - 10.1067/moe.2000.105173
IS - 5
KW - Zinc
PY - 2000
SN - 1079-2104 1528-395X
SP - 623-629
ST - Reaction of bony tissue to implanted silver glass ionomer and a reinforced
zinc oxide-eugenol cement
T2 - ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
TI - Reaction of bony tissue to implanted silver glass ionomer and a reinforced
VL - 89
Y2 - 5
ID - 9144
ER -
TY - JOUR
AB - OBJECTIVE: To evaluate the tissue responses to implants of Ketac Silver and
Super EBA cement in the guinea pig mandible. STUDY DESIGN: Sixteen guinea pigs were
used for 2 experimental periods of 4 and 12 weeks. Both materials were placed in
Teflon applicators and implanted into surgically prepared sites in the mandible. A
histologic examination for reaction to the material occurred after the animals were
killed and processed. RESULTS: After 4 weeks, minimal inflammatory reactions were
observed in Ketac Silver implants, whereas the Super EBA implants showed minimal to
moderate inflammation. Localized foreign body reaction was present in areas of
fragmented small particles of Ketac Silver. At 12-weeks observation, no
inflammatory reactions were present around either material. Direct bone contact was
observed in 1 Ketac Silver implant. CONCLUSIONS: Ketac Silver and Super EBA cement
elicited mild reactions under the conditions of this model. From a biologic point
of view, these 2 materials may offer equal utility in endodontic surgery.
AN - rayyan-553782118
AU - Pissiotis, E.
AU - Spângberg, L.
DO - 10.1067/moe.2000.105173
IS - 5
J2 - Oral Surg Oral Med Oral Pathol Oral Radiol Endod
KW - Animals
Bone and Bones/*drug effects
Cermet Cements/*toxicity
Dentin-Bonding Agents/*toxicity
Guinea Pigs
Male
Mandible/drug effects
Zinc
LA - eng
N1 - Department of Endodontology, School of Dental Medicine, University of
Connecticut Health Center, Framington, Connecticut, USA.
PY - 2000
SN - 1079-2104 (Print)
SP - 623-9
ST - Reaction of bony tissue to implanted silver glass ionomer and a reinforced
T2 - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
TI - Reaction of bony tissue to implanted silver glass ionomer and a reinforced
VL - 89
Y2 - 5
ID - 10029
ER -
TY - JOUR
AB - Background: Poplar leaf-buds (Populi gemmae) are used traditionally as anti-
inflammatory agents to the treatment of skin injuries or cough. They differ in
their diverse chemical composition and different types of activities, whose
mechanisms are not fully recognized. Purpose: Evaluation and comparison of anti-
inflammatory activity of leaf-buds extracts from Populus nigra, P. x berolinensis
and P. lasiocarpa and flavanones - pinocembrin and pinostrobin towards human
gingival fibroblasts (HGF-1) pro-inflammatory stimulated by silver nanoparticles
(AgNPs). Determination of antioxidant activity associated with anti-inflammatory
properties by means of bioautographic TLC tests. Methods: Phytochemical analysis
was performed by TLC and videodensitometry analysis. The extracts were standardized
on the pinocembrin and pinostrobin content. Bioautography was performed using 2,2-
diphenyl-1-picrylhydrazyl (DPPH) and riboflavin-light blue tetrazolium chloride
(riboflavin-light-NBT) radicals to assess the extracts and both flavanones radical
scavenging properties as well as potential inhibition of xanthine oxidase (XO)
activity. The protective effects of poplar buds extract and flavanones -
pinocembrin and pinostrobin on HGF-1 line exposured to AgNPs were investigated by
analysis of interleukin 6 (IL-6) and interleukin IL-1 beta (IL-1 beta) level
measured by ELISA kit. The messenger ribonucleic acid (mRNA) of both cytokines was
determined by real-time quantitative PCR. The involvement of cyclooxygenase 2
protein (COX-2) was studied using Western blot analysis. Results: The presence of
several flavanones and phenolic acids, which have radical scavenging properties,
was revealed in all of the bud poplar extracts analyzed. Treatment with particular
flavanones or extracts from buds of P. x berolinensis and P. nigra decreased the
IL-6 and IL-1 beta release in HGF-1 cells and down-regulation of mRNA for both
cytokines was observed. The COX-2 protein expression was demonstrated for
pinocembrin and P. x berolinensis buds. These effects were not observed for buds
from P. lasiocarpa not containing of flavonoids. Conclusion: The potential
protective role of pinocembrin and pinostrobin and extracts from buds P. nigra and
P. x berolinensis against AgNPs induced inflammation and cytotoxicity in HGF-1
cells is disclosed. In addition, the antioxidant properties of poplar bud extracts
have been demonstrated. P. x berolinensis buds showed the highest activity in both
the in vitro model and in the bioautographic tests.
AN - rayyan-553781198
AU - Poblocka-Olech, L.
AU - Inkielewicz-Stepniak, I.
AU - Krauze-Baranowska, M.
DO - 10.1016/j.phymed.2018.08.015
KW - Humanities
Humanism
Humans
Antioxidants
PY - 2019
SN - 0944-7113
SP - 1-9
ST - Anti-inflammatory and antioxidative effects of the buds from different
species of Populus in human gingival fibroblast cells: Role of bioflavanones
T2 - PHYTOMEDICINE
TI - Anti-inflammatory and antioxidative effects of the buds from different
species of Populus in human gingival fibroblast cells: Role of bioflavanones
VL - 56
Y2 - 3 y3 - 15
ID - 9146
ER -
TY - JOUR
AB - Nanotechnology has led to the development of numerous new systems for drug
delivery into the target tissue, as well as novel methods that may be useful in the
treatment of liver fibrosis. Inorganic and organic nanoparticles (NPs) are
currently used in medical investigations and in the treatment of liver diseases,
with adverse reactions observed in some cases. A revised treatment procedure
involving NPs is necessary to develop future drug delivery systems having minimal
noxious effects on the hepatic cells that take up and metabolize these particles in
a different manner, in order to find the medication that is capable of blocking and
even reversing fibrosis in an inflamed liver. In addition, the administered
medication should not induce systemic responses against the NPs used in the
treatment. © 2020, Polish Physiological Society. All rights reserved.
AN - rayyan-553781207
AU - Pop, D. D. Z.
AU - Mitrea, D. R.
AU - Suciu, S.
AU - Clichici, S. V.
DO - 10.26402/jpp.2020.6.01
IS - 6
KW - Liver fibrosis
Nanoparticle delivery systems
Nanoparticles
Nanotherapy
Nonalcoholic fatty liver disease
Nonalcoholic steatohepatitis
Stellate cells
Therapeutic nanomaterials
actin
albumin
antioxidant loaded nanoparticle
calcium phosphate nanoparticle
capecitabine
carbon nanotube nanoparticle
carboxymethylcellulose docetaxel conjugated nanoparticle
cisplatin
collagen
curcumin
curcumin loaded nanoparticle
desmin
dexamethasone loaded liposome
doxorubicin
fullerene nanoparticle
glucose transporter
glycyrrhizic acid
gold nanoparticle
graphene nanoparticle
Hermes antigen
hyaluronic acid micelle
inorganic nanoparticle
interleukin 6
lipid nanoparticle
macrogol
manganese oxide nanoparticle
metal nanoparticle
microRNA loaded nanoparticle
nanoencapsulated carvacrol nanoparticle
nanomaterial
naringenin loaded nanoparticle
organic anionic transporter
organic nanoparticle
peroxisome proliferator activated receptor gamma
procollagen
quercetin loaded nanoparticle
resveratrol
resveratrol loaded nanoparticle
silibinin
silica oxide nanoparticle
silver nanoparticle
silymarin
silymarin encapsulated nanoparticle
silymarin loaded liposome
sodium dependent multivitamin transporter
Sorafenib loaded poly actic co glycolic acid nanoparticle
unclassified drug
virus vector
Vitamin A coupled liposome
Article
biocompatibility
biomineralization
blood brain barrier
epithelium cell
hepatic stellate cell
hepatitis
human
hydrophobicity
inflammatory cell
Kupffer cell
lipid peroxidation
liver fibrosis
long term care
macrophage
nanoemulsion
nanotechnology
nonhuman
oxidative stress
particle size
phagocytosis
pinocytosis
self microemulsifying drug delivery system
SH-SY5Y cell line
Liver Cirrhosis
Liver
Nanostructures
PY - 2020
ST - Nanostructure-based therapies for liver fibrosis
T2 - Journal of Physiology and Pharmacology
TI - Nanostructure-based therapies for liver fibrosis
85103860053&doi=10.26402%2fjpp.2020.6.01&partnerID=40&md5=b1b84f6e9e92b2140a11f0605
c66ff82
VL - 71
ID - 9154
ER -
TY - JOUR
AB - Anecdotal reports in the press and epidemiological studies suggest that
deployment to Iraq and Afghanistan may be associated with respiratory diseases and
symptoms in U.S. military personnel and veterans. Exposures during military
operations were complex, but virtually all service members were exposed to high
levels of respirable, geogenic dust. Inhalation of other dusts has been shown to be
associated with adverse health effects, but the pulmonary toxicity of ambient dust
from Iraq has not been previously studied. The relative toxicity of Camp Victory
dust was evaluated by comparing it to particulate matter from northern Kuwait, a
standard U.S. urban dust, and crystalline silica using a single intratracheal
instillation in rats. Lung histology, protein levels, and cell counts were
evaluated in the bronchoalveolar lavage fluid 1-150 d later. The Iraq dust provoked
an early significant, acute inflammatory response. However, the level of
inflammation in response to the Iraq dust, U.S. urban dust, and Kuwait dust rapidly
declined and was nearly at control levels by the end of the study At later times,
animals exposed to the Iraq, U.S. urban, or Kuwait dusts showed increased small
airway remodeling and emphysema compared to silica-exposed and control animals
without evidence of fibrosis or premalignant changes. The severity and persistence
of pulmonary toxicity of these three dusts from the Middle East resemble those of a
U.S. urban dust and are less than those of silica. Therefore, Iraq dust exposure is
not highly toxic, but similar to other poorly soluble low-toxicity dusts.
AN - rayyan-553781208
AU - Porter, K. L.
AU - Green, F. H. Y.
AU - Harley, R. A.
AU - Vallyathan, V.
AU - Castranova, V.
AU - Waldron, N. R.
AU - Leonard, S. S.
AU - Nelson, D. E.
AU - Lewis, J. A.
AU - Jackson, D. A.
DO - 10.1080/15287394.2015.1072611
IS - 23
PY - 2015
SN - 1528-7394 1087-2620
SP - 1385-1408
ST - EVALUATION OF THE PULMONARY TOXICITY OF AMBIENT PARTICULATE MATTER FROM CAMP
VICTORY, IRAQ
T2 - JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
TI - EVALUATION OF THE PULMONARY TOXICITY OF AMBIENT PARTICULATE MATTER FROM CAMP
VICTORY, IRAQ
VL - 78
Y2 - 12 y3 - 17
ID - 9155
ER -
TY - JOUR
AB - Metals enriched in ambient air fine particulate matter (PM2.5) are thought to
contribute to the pathogenesis of PM2.5-induced inflammatory lung diseases. An
important mechanism involved in metal-induced lung injury involves increased
oxidative stress due to generation of reactive oxygen species. The redox sensitive
transcription factor, nuclear factor kappa B (NF-kappa B) converts extracellular
oxidative stress signals into changes in expression of genes associated with
diverse cellular activities. The purpose of this study was to determine the
mechanism by which exposure to Fe or Se, at environmentally relevant
concentrations, leads to an increased release of chemokines by cultured human lung
epithelial cells (A549). We tested the hypothesis that NF-kappa B signaling pathway
is involved in the metal induced IL-8 and MCP-1 release by Fe and Se. Exposure to
Fe or Se induced an enhanced release of chemokines at 6 and 24 h, and mediated
nuclear translocation of NF-kappa B. Levels of chemokines in response to Fe were
significantly suppressed in the presence of BMS-345541, a specific inhibitor of NF-
kappa B. Similar effects were seen in response to Se, indicating the involvement of
NF-kappa B in the metal-induced chemokine release, while not affecting the AP-1 c-
Jun-DNA binding activity. Overall, results indicate that both Fe and Se, at ambient
levels, possess the potential for inducing lung inflammation via an oxidative
stress pathway in lung epithelial cells.
AN - rayyan-553781209
AU - Potnis, P. A.
AU - Mitkus, R.
AU - Elnabawi, A.
AU - Squibb, K.
AU - Powell, J. L.
DO - 10.1039/c3tx50012h
IS - 4
Selenium
NF-kappa B
Lung
Oxalic Acid
Norisoprenoids
Iron
PY - 2013
SN - 2045-452X 2045-4538
SP - 259-269
ST - Role of NF-kappa B in the oxidative stress-induced lung inflammatory response
to iron and selenium at ambient levels
T2 - TOXICOLOGY RESEARCH
TI - Role of NF-kappa B in the oxidative stress-induced lung inflammatory response
to iron and selenium at ambient levels
VL - 2
ID - 9156
ER -
TY - JOUR
AB - Increasing use of engineered nanomaterials (ENM) in consumer products and
commercial applications has helped drive a rise in research related to the
environmental health and safety (EHS) of these materials. Within the cacophony of
information on ENM EHS to date are data indicating that these materials may be
neurotoxic in adult animals. Evidence of elevated inflammatory responses, increased
oxidative stress levels, alterations in neuronal function, and changes in cell
morphology in adult animals suggests that ENM exposure during development could
elicit developmental neurotoxicity (DNT), especially considering the greater
vulnerability of the developing brain to some toxic insults. In this review, we
examine current findings related to developmental neurotoxic effects of ENM in the
context of identifying research gaps for future risk assessments. The basic risk
assessment paradigm is presented, with an emphasis on problem formulation and
assessments of exposure, hazard, and dose response for DNT. Limited evidence
suggests that in utero and postpartum exposures are possible, while fewer than 10
animal studies have evaluated DNT, with results indicating changes in synaptic
plasticity, gene expression, and neurobehavior. Based on the available information,
we use current testing guidelines to highlight research gaps that may inform ENM
research efforts to develop data for higher throughput methods and future risk
assessments for DNT. Although the available evidence is not strong enough to reach
conclusions about DNT risk from ENM exposure, the data indicate that consideration
of ENM developmental neurotoxic potential is warranted. © Published by Oxford
University Press on behalf of the Society of Toxicology 2013.
AN - rayyan-553781210
AU - Powers, C. M.
AU - Bale, A. S.
AU - Kraft, A. D.
AU - Makris, S. L.
AU - Trecki, J.
AU - Cowden, J.
AU - Hotchkiss, A.
AU - Gillespie, P. A.
DO - 10.1093/toxsci/kft109
IS - 2
KW - Developmental neurotoxicity
Risk assessment research
Animals
Humans
Nanostructures
Nervous System
Risk Assessment
developmental neurotoxicity
engineered nanomaterials
risk assessment research.
copper
iron
magnetite
manganese
nanomaterial
polystyrene
povidone
silver
article
behavior change
blood brain barrier
blood placenta barrier
brain weight
developmental toxicity
gene expression
growth
health hazard
hearing
human
in vitro study
in vivo study
inflammation
learning
memory
motor performance
nanoengineering
nerve cell plasticity
neuropathology
neurotoxicity
nonhuman
planning
practice guideline
prenatal exposure
puerperium
risk assessment
startle reflex
survival
toxicity testing
Humanities
Humanism
PY - 2013
SP - 225-242
ST - Developmental neurotoxicity of engineered nanomaterials: Identifying research
needs to support human health risk assessment
T2 - Toxicological Sciences
TI - Developmental neurotoxicity of engineered nanomaterials: Identifying research
needs to support human health risk assessment
84880693748&doi=10.1093%2ftoxsci
%2fkft109&partnerID=40&md5=73e6f92110e4f75124825230563b1fb1
VL - 134
ID - 9157
ER -
TY - JOUR
AB - Nano silver particle (NSPs), is one of the most attractive nano materials,
and has been widely used in a range of biomedical applications, including
diagnosis, treatment, drug delivery, medical device coating, and for personal
health care. Silver nanoparticles can be synthesized by various methods but citrate
reduced and stabilized particles gain an advantage over other methods because of
its morphology, stable nature, cost effective and easy scale-up nature. In the
present study, the silver nanoparticle was synthesized by reducing silver salt with
sodium citrate that showed the characteristic peak at 410 nm by means of UV-Visible
spectroscopy. Antibacterial activity was performed against S.aureus and
P.aeruginosa, and S.aureusand was found to be more susceptible. Characterized
colloidal silver nanoparticles were used for the coating process. Coating on copper
plate was achieved by Dip method, and characterized using SEM, EDAX and FTIR.
Resulting EDAX and SEM images showed the morphology of the coated surface with
their elemental composition. FTIR interferogram showed the characteristic
functional groups at 1290.09 cm(-1) and 1483.68cm(-1) responsible for the reduction
of silver salt into silver nanoparticles. Antimicrobial activity was performed for
the coated metal against the Trichomonasvaginalis, a sexual transmitted pathogen
that attribute to pelvic inflammatory disease (PID).The inhibition diameter is of
about 31mm, thereby considering the fabricated nano composite to be effective
against the disease causing microbial species. Invitro cytotoxicity studies in the
Vero cell line show 81% viability with no significant morphological alterations.
Hence, the fabricated nano composite could be a future benchmark for implant
functionalization.
AN - rayyan-553781211
AU - Prabasheela, B.
AU - Sakithya, V.
DO - 10.22376/ijpbs/lpr.2020.10.5.L127-137
IS - 5
KW - Copper
PY - 2020
SN - 2250-0480
SP - L127-L137
ST - Fabrication of Silver Nanocomposite on Copper Metal- A Potential Strategy to
Alleviate Pelvic Inflammatory Disease
T2 - INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH
TI - Fabrication of Silver Nanocomposite on Copper Metal- A Potential Strategy to
Alleviate Pelvic Inflammatory Disease
VL - 10
Y2 - 12
ID - 9158
ER -
TY - JOUR
AB - The biosynthesis of silver nanoparticles using plant extracts is a safe and
efficient method. Human periodontal stem cells (hPDLSCs) can promote periodontal
tissue formation for regenerative therapy. The current study aimed to biosynthesize
silver nanoparticles (AgNPs) using stem bark extracts from Oroxylum indicum (L)
Kurz (OI) as a reducing agent (OI/AgNPs) and investigate the biological properties
of OI/AgNPs on human periodontal ligament stem cells (hPDLSCs). The OI/AgNPs were
biosynthesized and characterized using UV–vis spectrophotometry (UV‒vis), Fourier
transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission
electron microscopy (TEM) and zeta potential analyses. The hPDLSCs were stimulated
with hydrogen peroxide (H2O2, H2O2-hPDLSCs) and bacterial lipopolysaccharide (LPS,
LPS-hPDLSCs) and encapsulated in the hydrogel. After that, the levels of cell
viability, interleukin-1beta (IL-1b), alkaline phosphatase (ALP) activity, and
calcium content were measured and compared with quercetin (QT) as a reference
flavonoid. The OI/AgNPs were stable spherical nanoparticles with a size range of
21.49 ± 0.32 nm, and biosynthesis enhanced the antioxidant and biological effects
of the OI/AgNPs. The accumulation of OI/AgNPs in the cytoplasm of hPDLSCs with high
levels of cell viability was demonstrated. OI/AgNPs increased the cell growth of
H2O2-hPDLSCs and decreased the levels of IL-1b secretion from LPS-hPDLSCs. The
OI/AgNPs increased the ALP activity and calcium content of the hPDLSCs. In
conclusion, the biosynthesized OI/AgNPs were noncytotoxic and could protect hPDLSCs
against oxidative stress and inflammatory stimuli and promote osteoblastic
differentiation; thus, they are applicable for the regenerative treatment of
peri-implantitis. © 2022 The Author(s)
AN - rayyan-553781214
AU - Prapaipittayakhun, J.
AU - Boonyuen, S.
AU - Zheng, A. L. T.
AU - Apinyauppatham, K.
AU - Arpornmaeklong, P.
DO - 10.1016/j.onano.2022.100117
KW - Anti-inflammatory agent
Cell differentiation
Cell encapsulation
Flavonoids
Multifunctional nanoparticle
Somatic adult stem cells
bacterium lipopolysaccharide
biological product
calcium
flavonoid
hydrogen peroxide
interleukin 1beta
Oroxylum indicum extract
plant extract
quercetin
silver nanoparticle
unclassified drug
Article
bioassay
biological activity
bone characteristics and functions
cell encapsulation
cell growth
cell isolation
cell viability
controlled study
cytotoxicity
encapsulation
flow cytometry
human
human cell
IC50
Oroxylum
osteogenic differentiation
oxidative stress
particle size
periodontal ligament stem cell
physical chemistry
protein expression
stem cell expansion
total flavonoid content assay
X ray diffraction
zeta potential
Humanities
Humanism
Humans
Periodontal Ligament
PY - 2023
ST - Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on
human periodontal ligament stem cells
T2 - OpenNano
TI - Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on
human periodontal ligament stem cells
85144519025&doi=10.1016%2fj.onano.2022.100117&partnerID=40&md5=c1e3597a38c4a24b0f28
b17ee2797848
VL - 9
ID - 9161
ER -
TY - JOUR
AB - We showed previously that exposure of human lung cells (BEAS-2B) to TiO2
nanoparticles (nano-TiO2) produced micronuclei (MN) only when the final
concentration of protein in the cell-culture medium was at least 1%. Nanoparticles
localize in the liver; thus, we exposed human liver cells (HepG2) to nano-TiO2 and
found the same requirement for MN induction. Nano-TiO2 also formed small
agglomerates in medium containing as little as 1% protein and caused cellular
interaction as measured by side scatter by flow cytometry and DNA damage (comet
assay) in HepG2 cells. Nano-TiO2 also increased the activity of the inflammatory
factor NFkB but not of AP1 in a reporter-gene HepG2 cell line. Suspension of nano-
TiO2 in medium containing 0.1% protein was sufficient for induction of MN by the
nanoparticles in either BEAS-2B or HepG2 cells as long the final concentration of
protein in the cell-culture medium was at least 1%. Environ. Mol. Mutagen. 55:336-
342, 2014. (c) 2014 Wiley Periodicals, Inc.
AN - rayyan-553781216
AU - Prasad, R. Y.
AU - Simmons, S. O.
AU - Killius, M. G.
AU - Zucker, R. M.
AU - Kligerman, A. D.
AU - Blackman, C. F.
AU - Fry, R. C.
AU - DeMarini, D. M.
DO - 10.1002/em.21848
IS - 4
KW - Culture Media
Titanium
Cells, Cultured
PY - 2014
SN - 0893-6692 1098-2280
SP - 336-342
ST - Cellular Interactions and Biological Responses to Titanium Dioxide
Nanoparticles in HepG2 and BEAS-2B Cells: Role of Cell Culture Media
T2 - ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
TI - Cellular Interactions and Biological Responses to Titanium Dioxide
Nanoparticles in HepG2 and BEAS-2B Cells: Role of Cell Culture Media
VL - 55
Y2 - 5
ID - 9163
ER -
TY - JOUR
AB - Injectable, drug-releasing hydrogel scaffolds with multifunctional properties
including hemostasis and anti-bacterial activity are essential for successful wound
healing; however, designing ideal materials is still challenging. Herein, we
demonstrate the fabrication of a biodegradable, temperature-pH dual responsive
supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl
caprolactam) (AG/PVCL) through free radical polymerization and the subsequent
chemical and ionic cross-linking. A natural therapeutic molecule, tannic acid (TA)-
incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound
healing efficiency were studied. In the AG/PVCL-TA system, TA acts as a therapeutic
molecule and also substitutes as an effective gelation binder. Notably, the
polyphenol-arm structure and diverse bonding abilities of TA can hold polymer
chains through multiple bonding and co-ordinate cross-linking, which were vital in
the formation of the mechanically robust AG/PVCL-TA. The SHG formation was
successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a
sustained manner under physiological temperature-pH conditions. AG/PVCL-TA
displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and
cell proliferation activity towards the 3T3 fibroblast cell line. The wound healing
performance of AG/PVCL-TA was further confirmed in skin excision wound models,
which demonstrated the potential application of AG/PVCL-TA for skin regeneration
and rapid wound healing.
AN - rayyan-553781217
AU - Preman, N. K.
AU - Priya, S. E. S.
AU - Prabhu, A.
AU - Shaikh, S. B.
AU - Vipin, C.
AU - Barki, R. R.
AU - Bhandary, Y. P.
AU - Rekha, P. D.
AU - Johnson, R. P.
DO - 10.1039/d0tb01468k
IS - 37
KW - Wound Healing
Hemostasis
Infection Control
PY - 2020
SN - 2050-750X 2050-7518
SP - 8585-8598
ST - Bioresponsive supramolecular hydrogels for hemostasis, infection control and
accelerated dermal wound healing
TI - Bioresponsive supramolecular hydrogels for hemostasis, infection control and
accelerated dermal wound healing
VL - 8
Y2 - 10 y3 - 7
ID - 9164
ER -
TY - JOUR
AB - This study aimed to investigate the antioxidant, anti-inflammatory and
biosorption properties of starch nanocrystals (SNC). The characterization of
synthesized SNC was done using various analytical techniques like microscopic and
spectroscopic analysis. The antioxidant property was determined using DPPH (2,2-
diphenyl-1-picrylhydrazyl) assay and metal ion chelating assay. SNC showed the
highest scavenging activity of 70.03 +/- 0.74% at 100 mu g/mL concentration.
Protein denaturation assay and proteinase inhibitory assay depicted the anti-
inflammatory property of SNC. The results revealed that the maximum inhibition
activity was found at 100 mu g/mL with 72.71% inhibition. The maximum removal
efficiency was found to be 83.42% at pH 2.0 with 0.15 g biosorbent. As the pH
increases, biosorption capacity of SNC were reduced from 8.17 to 6.30 mg/g and the
efficiency of the dye removal was decreased from 80.95 to 36.01%. The shape of
synthesized SNC was spherical nanoplatelets and it shows agglomeration. The
Langmuir isotherm model is best suited for the biosorption experiments with the R2
value of 0.986. SNC were subjected to cytotoxic and phytotoxic evaluation. Cell
viability and phytotoxic assays proves the non-toxic nature of the SNC.
AN - rayyan-553781218
AU - Priyan, V. V.
AU - Shahnaz, T.
AU - Kunnumakkara, A. B.
AU - Rana, V.
AU - Saravanan, M.
AU - Narayanasamy, S.
DO - 10.1007/s10876-020-01905-5
IS - 5
KW - Antioxidants
PY - 2021
SN - 1040-7278 1572-8862
SP - 1419-1430
ST - Antioxidant, Anti-inflammatory and Biosorption Properties of Starch
Nanocrystals In Vitro Study: Cytotoxic and Phytotoxic Evaluation
TI - Antioxidant, Anti-inflammatory and Biosorption Properties of Starch
Nanocrystals In Vitro Study: Cytotoxic and Phytotoxic Evaluation
VL - 32
Y2 - 9
ID - 9165
ER -
TY - JOUR
AB - ABSTRACT Objectives To describe acute and sub acute aspects of histological
and immunohistochemical response to PP implant in a rat subcutaneous model based on
objective methods. Materials and Methods Thirty rats had a PP mesh subcutaneously
implanted and the same dissection on the other side of abdomen but without mesh
(sham). The animals were euthanized after 4 and 30 days. Six slides were prepared
using the tissue removed: one stained with hematoxylin-eosin (inflammation
assessment); one unstained (birefringence evaluation) and four slides for
immunohistochemical processing: IL-1 and TNF-α (pro-inflammatory cytokines), MMP-2
(collagen metabolism) and CD-31 (angiogenesis). The area of inflammation, the
birefringence index, the area of immunoreactivity and the number of vessels were
objectively measured. Results A larger area of inflammatory reaction was observed
in PP compared to sham on the 4th and on the 30th day (p=0.0002). After 4 days, PP
presented higher TNF (p=0.0001) immunoreactivity than sham and no differences were
observed in MMP-2 (p=0.06) and IL-1 (p=0.08). After 30 days, a reduction of IL-1
(p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed.
Moreover, area of MMP-2 immunoreactivity decreased over time for PP group
(p=0.018). Birefringence index and vessel counting showed no differences between PP
and sham (p=0.27 and p=0.58, respectively). Conclusions The implantation of
monofilament and macroporous polypropylene in the subcutaneous of rats resulted in
increased inflammatory activity and higher TNF production in the early post implant
phase. After 30 days, PP has similar cytokines immunoreactivity, vessel density and
extracellular matrix organization.
AN - rayyan-553781219
AU - Prudente, Alessandro
AU - Fávaro, Wágner José
AU - Latuf Filho, Paulo
AU - Riccetto, Cássio Luis Zanettini
DO - 10.1590/S1677-5538.IBJU.2015.0289
IS - 3
KW - Foreign body reaction
Graft versus host reaction
Pelvic organ prolapse
Polypropylenes
Urinary incontinence
Rats
LA - en
PY - 2016
SN - 1677-5538
SP - 585-593
ST - Host inflammatory response to polypropylene implants: insights from a
quantitative immunohistochemical and birefringence analysis in a rat subcutaneous
model
T2 - Int. braz. j. urol
TI - Host inflammatory response to polypropylene implants: insights from a
quantitative immunohistochemical and birefringence analysis in a rat subcutaneous
model
55382016000300585
VL - 42
Y2 - 6 y3 - 1
ID - 9166
ER -
TY - JOUR
AB - Dental implants with proper antibacterial ability as well as ideal
osseointegration are being actively pursued. The antimicrobial ability of titanium
implants can be significantly enhanced via modification with silver nanoparticles
(Ag NPs). However, the high mobility of Ag NPs results in their potential
cytotoxicity. The silver plasma immersion ion-implantation (Ag-PIII) technique may
remedy the defect. Accordingly, Ag-PIII technique was employed in this study in an
attempt to reduce the mobility of Ag NPs and enhance osseointegration of
sandblasted and acid-etched (SLA) dental implants. Briefly, 48 dental implants,
divided equally into one control and three test groups (further treated by Ag-PIII
technique with three different implantation parameters), were inserted in the
mandibles of six Labrador dogs. Scanning electron microscopy, X-ray photoelectron
spectroscopy, and inductively coupled plasma optical emission spectrometry were
used to investigate the surface topography, chemical states, and silver release of
SLA- and Ag-PIII-treated titanium dental implants. The implant stability quotient
examination, Microcomputed tomography evaluation, histological observations, and
histomorphometric analysis were performed to assess the osseointegration effect in
vivo. The results demonstrated that normal soft tissue healing around dental
implants was observed in all the groups, whereas the implant stability quotient
values in Ag-PIII groups were higher than that in the SLA group. In addition, all
the Ag-PIII groups, compared to the SLA-group, exhibited enhanced new bone
formation, bone mineral density, and trabecular pattern. With regard to osteogenic
indicators, the implants treated with Ag-PIII for 30 minutes and 60 minutes, with
the diameter of the Ag NPs ranging from 5-25 nm, were better than those treated
with Ag-PIII for 90 minutes, with the Ag NPs diameter out of that range. These
results suggest that Ag-PIII technique can reduce the mobility of Ag NPs and
enhance the osseointegration of SLA surfaces and have the potential for future use.
AN - rayyan-553781223
AU - Qiao, S. C.
AU - Cao, H. L.
AU - Zhao, X.
AU - Lo, H. W.
AU - Zhuang, L. F.
AU - Gu, Y. X.
AU - Shi, J. Y.
AU - Liu, X. Y.
AU - Lai, H.
DO - 10.2147/IJN.S73467
KW - Dogs
Animals
Titanium
Dental Implantation
Dental Implants
Aged
Aging
Animal Shells
PY - 2015
SN - 1178-2013
SP - 653-664
ST - Ag-plasma modification enhances bone apposition around titanium dental
implants: an animal study in Labrador dogs
TI - Ag-plasma modification enhances bone apposition around titanium dental
implants: an animal study in Labrador dogs
VL - 10
ID - 9170
ER -
TY - JOUR
AB - Macrophages are becoming increasingly significant in the progression of
atherosclerosis (AS). Molecular imaging of macrophages may improve the detection
and characterization of AS. In this study, dendrimer-entrapped gold nanoparticles
(Au DENPs) with polyethylene glycol (PEG) and fluorescein isothiocyanate (FI)
coatings were designed, tested, and applied as contrast agents for the enhanced
computed tomography (CT) imaging of macrophages in atherosclerotic lesions. Cell
counting kit-8 assay, fluorescence microscopy, silver staining, and transmission
electron microscopy revealed that the FI-functionalized Au DENPs are noncytotoxic
at high concentrations (3.0 μM) and can be efficiently taken up by murine
macrophages in vitro. These nanoparticles were administered to apolipoprotein E
knockout mice as AS models, which demonstrated that the macrophage burden in
atherosclerotic areas can be tracked noninvasively and dynamically three-
dimensionally in live animals using micro-CT. Our findings suggest that the
designed PEGylated gold nanoparticles are promising biocompatible nanoprobes for
the CT imaging of macrophages in atherosclerotic lesions and will provide new
insights into the pathophysiology of AS and other concerned inflammatory diseases.
AN - rayyan-553782220
AU - Qin, J.
AU - Peng, C.
AU - Zhao, B.
AU - Ye, K.
AU - Yuan, F.
AU - Peng, Z.
AU - Yang, X.
AU - Huang, L.
AU - Jiang, M.
AU - Zhao, Q.
AU - Tang, G.
AU - Lu, X.
DO - 10.2147/IJN.S72819
KW - Animals
Atherosclerosis/*pathology
Cell Line
Erythrocytes/drug effects
Fluorescein-5-isothiocyanate/chemistry/pharmacokinetics/toxicity
Gold/*chemistry/pharmacokinetics/toxicity
Hemolysis/drug effects
Humans
Macrophages/*chemistry/cytology
Metal Nanoparticles/*chemistry/toxicity
Mice
Molecular Imaging/*methods
Polyethylene Glycols/chemistry
Tissue Distribution
Tomography, X-Ray Computed/*methods
Macrophages
LA - eng
N1 - Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated
to Shanghai JiaoTong University, School of Medicine, Shanghai, People's Republic of
China.; Department of Radiology, Shanghai Tenth People's Hospital Affiliated to
Tongji University, School of Medicine, Shanghai, People's Republic of China.;
Department of Radiology, Shanghai Tenth People's Hospital Affiliated to Tongji
University, School of Medicine, Shanghai, People's Republic of China.; Department
of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai
JiaoTong University, School of Medicine, Shanghai, People's Republic of China.;
Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to
Shanghai JiaoTong University, School of Medicine, Shanghai, People's Republic of
China.; Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated
China.; Department of Orthopaedics, Shanghai First People's Hospital, School of
Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.;
Department of Radiology, Shanghai Tenth People's Hospital Affiliated to Tongji
University, School of Medicine, Shanghai, People's Republic of China.; Department
of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai
JiaoTong University, School of Medicine, Shanghai, People's Republic of China ;
Vascular Center of Shanghai JiaoTong University, Shanghai, People's Republic of
China.
PY - 2014
SP - 5575-90
ST - Noninvasive detection of macrophages in atherosclerotic lesions by computed
tomography enhanced with PEGylated gold nanoparticles
TI - Noninvasive detection of macrophages in atherosclerotic lesions by computed
tomography enhanced with PEGylated gold nanoparticles
VL - 9
ID - 10130
ER -
TY - JOUR
AB - Diabetic wounds are one of the most challenging public health issues of the
21st century due to their inadequate vascular supply, bacterial infections, high
levels of oxidative stress, and abnormalities in antioxidant defenses, whereas
there is no effective treatment for diabetic wounds. Due to the distinct properties
of nanoparticles, such as their small particle size, elevated cellular uptake, low
cytotoxicity, antibacterial activity, good biocompatibility, and biodegradability.
The application of nanoparticles has been widely used in the treatment of diabetic
wound healing due to their superior anti-inflammatory, antibacterial, and
antioxidant activities. These nanoparticles can also be loaded with various agents,
such as organic molecules (eg, exosomes, small molecule compounds, etc.), inorganic
molecules (metals, nonmetals, etc.), or complexed with various biomaterials, such
as smart hydrogels (HG), chitosan (CS), and hyaluronic acid (HA), to augment their
therapeutic potential in diabetic wounds. This paper reviews the therapeutic
potential and future perspective of nanoparticles in the treatment of diabetic
wounds. Together, nanoparticles represent a promising strategy in the treatment of
diabetic wound healing. The future direction may be to develop novel nanoparticles
with multiple effects that not only act in wound healing at all stages of diabetes
but also provide a stable physiological environment throughout the wound-healing
process. © 2022 Qin et al.
AN - rayyan-553781225
AU - Qin, W.
AU - Wu, Y.
AU - Liu, J.
AU - Yuan, X.
AU - Gao, J.
DO - 10.2147/IJN.S386585
KW - biomaterials
diabetic complications
diabetic wound
nanoparticles
wound healing
Antioxidants
Chitosan
Diabetes Mellitus
Humans
Hydrogels
Nanoparticles
Wound Healing
botulinum toxin
Ceria Nanoparticle
chitosan
copper nanoparticle
Ganglioside monosialic acid 3
gelatinase A
gelatinase B
gold nanoparticle
heme oxygenase 1
heparin
hyaluronic acid
hydrogel
hypertensive factor
hypoxia inducible factor 1
hypoxia inducible factor 1alpha
interleukin 10
interleukin 12
interleukin 6
interleukin 8
methylcellulose
nanoparticle
nitric oxide
phosphatidylinositol 3 kinase
platelet derived growth factor B
polycaprolactone
polyethyleneimine
protein kinase B
pullulan
quercetin
Rubidium Nanoparticle
silica nanoparticle
silver nanoparticle
somatomedin C
stromal cell derived growth factor 1
transforming growth factor alpha
unclassified drug
vasculotropin
vasculotropin A
vasculotropin receptor 2
antiinfective agent
antioxidant
angiogenesis
bacterial infection
biocompatibility
biodegradability
cell migration
cell proliferation
cytokine production
cytokine release
cytotoxicity
diabetes mellitus
exosome
human
nonhuman
oxidative stress
protein expression
Review
signal transduction
vascular disease
PY - 2022
SP - 6007-6029
ST - A Comprehensive Review of the Application of Nanoparticles in Diabetic Wound
Healing: Therapeutic Potential and Future Perspectives
T2 - International Journal of Nanomedicine
TI - A Comprehensive Review of the Application of Nanoparticles in Diabetic Wound
Healing: Therapeutic Potential and Future Perspectives
85144584615&doi=10.2147%2fIJN.S386585&partnerID=40&md5=744e9631b75e443643dd450627ee
a2d4
VL - 17
ID - 9172
ER -
TY - JOUR
AB - BACKGROUND: Epidemiological studies have shown that exposure to ambient fine
particulate matter with aerodynamic diameter less than or equal to 2.5 μm (PM2:5 )
correlates with a decrease in sperm count, but the biological mechanism remains
elusive. OBJECTIVES: This study tested whether hypothalamic inflammation, an
emerging pathophysiological mediator, mediates the development of lower epididymal
sperm count due to PM2:5 exposure. METHODS: Inhibitor jB kinase 2 (IKK2) was
conditionally knocked out either in all neurons or subtypes of hypothalamic neurons
of mice. Effects of concentrated ambient PM2:5 (CAP) exposure on hypothalamic
inflammation, the hypothalamic–pituitary–gonadal (HPG) axis, and epididymal sperm
count of these mouse models were then assessed. Furthermore, to test whether
hypothalamic inflammation is sufficient to decrease sperm production, we
overexpressed constitutively active IKK2 (IKK2ca) either in all neurons or subtypes
of hypothalamic neurons and assessed hypothalamic inflam-mation, the HPG axis, and
sperm production of these overexpression mouse models. RESULTS: CAP-exposed wild-
type control mice vs. filtered air (FA)-exposed wild-type control mice had a higher
expression of hypothalamic inflammatory markers, lower functional indexes of the
HPG axis, and a lower epididymal sperm count. In contrast, all these measurements
for CAP-vs. FA-exposed mice deficient of IKK2 in all neurons were comparable. We
also found that overexpression of IKK2ca in either all neurons or pro-
opiomelanocortin (POMC) neurons only, but not in Agouti-related protein (AgRP)
neurons only, resulted in lower functional indexes of the HPG axis and a lower
epididymal sperm count. Moreover, we showed that CAP-vs. FA-exposed mice deficient
of IKK2 in POMC neurons had a comparable expression of hypothalamic inflammatory
markers, comparable functional indexes of the HPG axis, and a comparable epididymal
sperm count. DISCUSSION: This mouse model study shows a causal role of IKK2 of POMC
neurons in the development of lower epididymal sperm count due to PM2:5 exposure,
providing a mechanistic insight into this emerging pathogenesis.
https://doi.org/10.1289/EHP8868. © 2021, Public Health Services, US Dept of Health
and Human Services. All rights reserved.
AN - rayyan-553781228
AU - Qiu, L.
AU - Chen, M.
AU - Wang, X.
AU - Chen, S.
AU - Ying, Z.
DO - 10.1289/EHP8868
IS - 9
KW - Animals
Hypothalamus
Male
Mice
Neurons
Particulate Matter
Pro-Opiomelanocortin
Spermatogenesis
3(or 17)beta hydroxysteroid dehydrogenase
agouti related protein
aluminum
androgen receptor
barium
bromine
cadmium
calcium
cerium
cholesterol monooxygenase (side chain cleaving)
chromium
copper
cre recombinase
cytochrome P450
cytochrome P450 family 17 alpha
erbium
estradiol
follitropin
gold
gonadorelin
I kappa B kinase beta
interleukin 1beta
interleukin 6
iridium
iron
kappa B Kinase 2 inhibitor
lead
luteinizing hormone
lutetium
magnesium
manganese
mercury
messenger RNA
molybdenum
nestin
nickel
pentobarbital
phosphorus
platinum
potassium
praseodymium
proopiomelanocortin
protein inhibitor
rubidium
selenium
sex hormone binding globulin
silicon
silver
sodium
steroidogenic acute regulatory protein
strontium
testosterone
testosterone 17beta dehydrogenase
thallium
tin
titanium
tungsten
unclassified drug
vanadium
zinc
zirconium
adult
aerodynamics
animal cell
animal experiment
animal model
animal tissue
antigen retrieval
Article
chemiluminescence immunoassay
controlled study
environmental factor
epidemiological monitoring
gene overexpression
germ cell
hypothalamic inflammation
hypothalamus hypophysis system
hypothalamus nucleus
male
mass spectrometry
mouse
nerve cell culture
nerve cell differentiation
nonhuman
particulate matter
protein expression
semen analysis
seminiferous tubule
Sertoli cell
sperm count
spermatocele
spermatogenesis
spermatogonium
testis weight
versatile aerosol concentration enrichment system
animal
hypothalamus
metabolism
nerve cell
toxicity
PY - 2021
ST - Pm2:5 exposure of mice during spermatogenesis: A role of inhibitor jb kinase
2 in pro-opiomelanocortin neurons
T2 - Environmental Health Perspectives
TI - Pm2:5 exposure of mice during spermatogenesis: A role of inhibitor jb kinase
2 in pro-opiomelanocortin neurons
85115376790&doi=10.1289%2fEHP8868&partnerID=40&md5=112f04965cd7ce7b60b4bf0159e82aa4
VL - 129
ID - 9175
ER -
TY - GEN
AB - Os eventos histológicos no fígado foram quantificados, usando técnica de
imunomarcação para avaliar 53 amostras hepáticas provenientes de viscerotomia de
pacientes vitimados por febre amarela silvestre. A análise quantitativa dos eventos
demonstrou amplo predomínio do componente apoptótico sobre a necrose. O infiltrado
inflamatório é desproporcional em intensidade à morte dos hepatócitos. Houve
predomínio de linfócitos TCD4+, ocorrendo em menor proporção linfócitos TCD8+,
linfócitos B, células NK e apresentadoras de antígenos (S100). A expressão
citocínica foi de perfil Th1, com expressão de TNF-a, IFN-g e acompanhada de
intensa imunomarcação para TGF-b. Frente aos achados acreditamos que a predileção
pela localização médio zonal das lesões do fígado poderia decorrer de fenômenos de
hipóxia por hipofluxo secundária à vasculopatia sistêmica e associada ao efeito
citopático viral.In this work, the histological events in the liver were
quantified, using a immunomarking technique to evaluate 53 hepatic samples from the
viscerotomy of patients with the sylvatic form of yellow fever. Quantitative
analysis of the events demonstrated an ample prevalence of an apoptotic component
in the necrosis. The intensity of the inflammatory infiltration is disproportionate
to the death of the hepatocytes. There is a prevalence of TCD4+ lymphocytes, with a
smaller proportion of TCD8+ lymphocytes, B lymphocytes, NK cells and antigen-
presenting cells (S100). The cytokine expression presented a profile of Th1, with
expression of TNF-a, IFN-g and accompanied by intense immunomarking by TGF-b. Faced
with these findings, we consider that the predilection for the midzonal location of
the lesions in the liver could arise from the phenomena of hypoxia secondary to
systemic vasculopathy associated to the viral cytopathic effect...
AN - rayyan-553781229
AU - Quaresma, Juarez Antônio Simões
LA - pt
PY - 2003
SP - [162]-[162]
ST - Arboviroses da Amazônia: estudo do mecanismo de morte celular e da resposta
fenotípica do hospedeiro na febre amarela
TI - Arboviroses da Amazônia: estudo do mecanismo de morte celular e da resposta
fenotípica do hospedeiro na febre amarela
ID - 9176
ER -
TY - JOUR
AB - Tissue engineered vascular grafts (TEVGs) are beginning to achieve clinical
success and hold promise as a source of grafting material when donor grafts are
unsuitable or unavailable. Significant technological advances have generated small-
diameter TEVGs that are mechanically stable and promote functional remodeling by
regenerating host cells. However, developing a biocompatible blood-contacting
surface remains a major challenge. The TEVG luminal surface must avoid negative
inflammatory responses and thrombogenesis immediately upon implantation and promote
endothelialization. The surface has therefore become a primary focus for research
and development efforts. The current state of TEVGs is herein reviewed with an
emphasis on the blood-contacting surface. General vascular physiology and
developmental challenges and strategies are briefly described, followed by an
overview of the materials currently employed in TEVGs. The use of biodegradable
materials and stem cells requires careful control of graft composition, degradation
behavior, and cell recruitment ability to ensure that a physiologically relevant
vessel structure is ultimately achieved. The establishment of a stable monolayer of
endothelial cells and the quiescence of smooth muscle cells are critical to the
maintenance of patency. Several strategies to modify blood-contacting surfaces to
resist thrombosis and control cellular recruitment are reviewed, including coatings
of biomimetic peptides and heparin. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA,
Weinheim
AN - rayyan-553781230
AU - Radke, D.
AU - Jia, W.
AU - Sharma, D.
AU - Fena, K.
AU - Wang, G.
AU - Goldman, J.
AU - Zhao, F.
DO - 10.1002/adhm.201701461
IS - 15
KW - blood-contacting surfaces
endothelialization
surface modification
vascular engineering
vascular grafts
Animals
Blood Vessel Prosthesis
Blood Vessel Prosthesis Implantation
Collagen
Humans
Swine
Tissue Engineering
Tissue Scaffolds
Biocompatibility
Biomimetics
Cytology
Endothelial cells
Grafts
Muscle
Stem cells
Surface treatment
Tissue engineering
argatroban
CD133 antigen
CD34 antibody
CD59 antigen
collagen
complement component C1s inhibitor
complement factor H
complement factor I
decay accelerating factor
elastin
heparin
membrane cofactor protein
nitric oxide
politef
polycaprolactone
polyethylene terephthalate
polyglactin
polyglycolic acid
polylactic acid
silk fibroin
silver nanoparticle
thrombin
thrombomodulin
tropoelastin
Biodegra-dable materials
Contacting surfaces
Endothelialization
Research and development
Technological advances
Tissue engineered vascular grafts
Vascular engineering
Vascular grafts
biocompatibility
biodegradation
blood clotting
blood flow
blood pressure
blood vessel tone
bone marrow derived mononuclear cell
cell activation
cell engineering
colony forming cell
dynamic cell seeding
electrostatic cell seeding
endothelial colony forming cell
endothelium cell
enzyme activation
graft rejection
hemostasis
human
in vitro study
inflammation
magnetic cell seeding
nonhuman
priority journal
protein secretion
Review
static cell seeding
thrombocyte
thrombosis
tissue engineering
animal
blood vessel prosthesis
blood vessel transplantation
chemistry
pig
procedures
tissue scaffold
Blood
PY - 2018
ST - Tissue Engineering at the Blood-Contacting Surface: A Review of Challenges
and Strategies in Vascular Graft Development
TI - Tissue Engineering at the Blood-Contacting Surface: A Review of Challenges
and Strategies in Vascular Graft Development
85046403857&doi=10.1002%2fadhm.201701461&partnerID=40&md5=29eacdb603676b92c2cc1b4d7
9165dc9
VL - 7
ID - 9177
ER -
TY - JOUR
AB - Introduction: Silver nanomaterials; the engineered nanomaterials; have many
industrial applications. They are used in manufacture of cosmetic and daily used
products as food and clothes. Nanoparticles can pass through different biological
body barriers, accumulate in the female reproductive organs as uterus and ovaries
and exert their toxicity. Aim of the Work: To evaluate the effect of silver
nanoparticles on the histological structure of the endometrium using different
histological and immunohistochemical techniques. Materials and Methods: Thirty-six
adult female albino rats were divided into three equal groups; group I (control
group), group II and group III. Silver nanoparticles were administered daily for 2
weeks at dose 30mg /kg and 300mg/kg respectively orally to group II and III. The
size of the used nanoparticles is 20 nm. Specimens of uterus were taken to be
processed for examination by light microscope (haematoxylin and eosin, Masson
trichrome and TNF- alpha immunohistochemical staining) and transmission electron
microscope. Results: AgNps-treated animals showed stratification, cytoplasmic
vacuolation, discontinuation and desquamation in endometrial epithelial cells. The
lamina propria showed cellular infiltration (inflammatory reaction), empty spaces
and increase the deposition of collagen fibers (fibrosis). Ultrastructurally, they
showed focal loss of the apical microvilli of the endometrial epithelial cells,
apical cytoplasmic vacuoles, swollen, distorted and irregularly arranged
mitochondria and large amount of secondary lysosomes and autophagic vacuoles. Focal
separation of the epithelial cells with destruction of the intercellular junctions
was seen. The lamina propria showed accumulation of collagen fibres and
eosinophilic infiltration. Immunohistochemical study revealed highly significant
increase in the TNF- alpha immunoexpression particularly in group III. All these
changes were more severe in animals with high dose than those of low dose.
Conclusion: Silver nanoparticles induce structural changes in a dose dependent
manner in the endometrium. So, it should be given cautiously to females to avoid
uterine damage. EJH copyright © 2022. All rights served.
AN - rayyan-553781231
AU - Ragab, A. M. H.
AU - Ragab, M.
AU - Tawfik, S. M.
DO - 10.21608/ejh.2021.74346.1472
IS - 3
KW - Endometrium rat
proestrous phase
Rats
Endometrium
PY - 2022
SP - 756-773
ST - Histological and Immunohistochemical Alterations Induced by Exposure to
Different Doses of Silver Nanoparticles in the Endometrium of Adult Albino Rat
TI - Histological and Immunohistochemical Alterations Induced by Exposure to
Different Doses of Silver Nanoparticles in the Endometrium of Adult Albino Rat
85144770662&doi=10.21608%2fejh.2021.74346.1472&partnerID=40&md5=ff8a9ee4a52b936fd11
3505d178547f8
VL - 45
ID - 9178
ER -
TY - JOUR
AB - Bisphenol A ( BPA ) is chemical compound found in the environment and used in
the manufacturing of plastic, which known as deleterious part of different body
parts. Salvia officinalis ( SO ) which is perennial round shrub in the family of
Labiatae /Lamiaceae. It’s has potent antioxidant and anti-inflammatory effects. The
present study was conducted to evaluate the protective role of SO as nanoparticles
against harmful effect of BPA in female rats. Eighteen adult female rats were used
and divided equally and randomly into three groups as following: first group
received DMSO orally and served as control group while second and third groups were
gavaged either with BPA alone or in combination with SOSNP respectively, all the
treatments extend 30 days. The result revealed harm effect of BPA by significantly
increase the level of estrogen, AST, ALT, ALP, urea and creatinine with
significantly reduction in the FSH and LH. SOSNPs show ameliorative effect on all
above parameters. © 2020, Indian Journal of Forensic Medicine and Toxicology. All
rights reserved.
AN - rayyan-553781235
AU - Raheem, H. A.
AU - Mousa, R. F.
AU - Hassan, A. H.
IS - 2
KW - Bisphenol A
FSH
LH
Salvia officinalis
4,4' isopropylidenediphenol
antioxidant
creatinine
dimethyl sulfoxide
estrogen
follitropin
luteinizing hormone
Salvia officinalis extract
Salvia officinalis extract silver nanoparticle
silver nanoparticle
unclassified drug
urea
adult
animal experiment
animal model
animal tissue
Article
blood sampling
cell damage
controlled study
female
kidney function
liver function
nephrotoxicity
nonhuman
oxidative stress
rat
toxicity
Rats
PY - 2020
SP - 852-857
ST - Evaluation protective role of salvia officinalis silver nanoparticles against
toxic effect of bisphenol a in female rats
T2 - Indian Journal of Forensic Medicine and Toxicology
TI - Evaluation protective role of salvia officinalis silver nanoparticles against
toxic effect of bisphenol a in female rats
85087394103&partnerID=40&md5=853392813e22edb34ffab74f15a4df07
VL - 14
ID - 9182
ER -
TY - JOUR
AB - Hypericum perforatum is native to parts of Europe and Asia but has spread
worldwide as a cosmopolitan invasive weed, including to temperate regions of India,
China, Canada, Africa, and the United States. The aim of this study was to overview
its therapeutic effects. This review article was carried out by searching studies
in PubMed, Medline, Web of Science, and Iran Medex databases. The initial search
strategy identified about 98 references. In this study, 42 studies was accepted for
further screening and met all our inclusion criteria [in English, full text,
therapeutic effects of Hypericum perforatum and dated mainly from the year 1987 to
2016.The search terms were "Hypericum perforatum", lemon balm, "therapeutic
properties", "pharmacological effects". It is commonly used for antimicrobial
effect, neuroprotective effect, anti-depressive effect, antioxidant effect,
menopause, dental practice, anti-inflammatory, wound healing effect, anti-cancer
effect, anti-herpes effect, phototoxicological effect. Hypericum perforatum is
widely used for therapeutic and non-therapeutic purposes that trigger its
significant value. Various combinations and numerous medicinal properties of its
extract, oil, and leaves demand further and more studies about the other useful and
unknown properties of this multipurpose plant.
AN - rayyan-553781236
AU - Rahimi, R.
AU - Kiani, S.
IS - 9
KW - Alternative and complementary medicine
Hypericum perforatum
Pharmacognosy
Phytochemicals
Therapeutic effects
alpha tocopherol
antidepressant agent
Arnica montana extract
hyperforin
hypericin
Hypericum perforatum extract
neem oil
placebo
quercetin
sertraline
sulfadiazine silver
actinic keratosis
antidepressant activity
Article
basal cell carcinoma
Bowen disease
burn
depression
desquamation
drug efficacy
experimental surgical wound
head and neck cancer
Herpes simplex virus 1
Herpes simplex virus 2
human
major depression
menopausal syndrome
neuroprotection
nonhuman
phototoxicity
plant leaf
skin toxicity
tooth pain
wound healing
Hypericum
PY - 2016
SP - 237-241
ST - Chemical compound and therapeutic effects of Hypericum perforatum
T2 - Der Pharmacia Lettre
TI - Chemical compound and therapeutic effects of Hypericum perforatum
84976274997&partnerID=40&md5=542ad34e1e43b934a547af6d67c810d9
VL - 8
ID - 9183
ER -
TY - JOUR
AB - M2 polarization of macrophages is predominant in case of tumors and some
other infectious diseases for disease progression. Repolarization of the M2
phenotype to the M1 state may be required to cure diseases. Hence, it is of great
interest to find out a material that would repolarize the M2 phenotype to the M1
state. Herein, the arabinogalactan protein from Andrographis paniculata (ApAGP) was
used to prepare a silver nanoparticle-ApAGP (SNP-ApAGP) bioconjugate, which was
characterized via UV-vis spectroscopy, zeta potential analysis, FT-IR spectroscopy,
and HR-TEM. Studies suggest that SNP-ApAGP (2.5 mu g mL(-1)) up-regulates ROS
generation, NO generation, and pro-inflammatory cytokine release (IL-12, IFN-gamma,
TNF-alpha, and IL-6). SNP-ApAGP also down-regulates the arginase-1 activity and
anti-inflammatory cytokine release (IL-4 & IL-10) in M0, M1, and M2-polarized
peritoneal macrophages in vitro. Therefore, SNP-ApAGP induces M1 polarization in M0
macrophages, enhances the proinflammatory activity of the M1 phenotype, and can
also repolarize M2 macrophages into the M1 phenotype. Therefore, SNP-ApAGP could be
used for treating various infectious diseases and cancers where repolarization of
M2 macrophages may be required to cure the disease.
AN - rayyan-553781237
AU - Raja, M. R. C.
AU - Kumar, V. V.
AU - Srinivasan, V.
AU - Selvaraj, S.
AU - Radhakrishnan, N.
AU - Mukundan, R.
AU - Raghunandan, S.
AU - Anthony, S. P.
AU - Mahapatra, S. K.
DO - 10.1039/c6tb02095j
IS - 19
KW - Macrophages
PY - 2017
SN - 2050-750X 2050-7518
SP - 3511-3520
ST - ApAGP-fabricated silver nanoparticles induce amendment of murine macrophage
polarization
TI - ApAGP-fabricated silver nanoparticles induce amendment of murine macrophage
polarization
VL - 5
Y2 - 5 y3 - 21
ID - 9184
ER -
TY - JOUR
AB - Apoptosis, a physiological mechanism of highly orchestrated cell death, can
be initiated by extracellular and intracellular mechanisms that trigger a complex
machinery of proapoptotic proteases and mitochondrial changes, leading to the
activation of specific endonucleases and DNA fragmentation. The present study was
undertaken to elucidate a mechanism underlying the inhibitory effect of
biosynthesised silver nanoparticle on TNF-α induced NF-κB nuclear translocation in
prostate cancer PC- 3 cells. The cell cycle analysis of Prostate cancer PC-3 cells
was examined by flow cytometry by using annexin V-FITC/PI staining. Effect of
silver nanoparticles in oxidative stress ROS, Effect of biosynthesized silver
nanoparticle on apoptosis in human prostate cancer cell line and apoptotic
induction of TNF-α and NF-κB expression was studied by Flow cytometry in Prostate
cancer PC-3 cell line. From the results it was observed that biosynthesized silver
nanoparticle inhibits the cellular growth of human prostate cancer PC-3 cells and
induces apoptosis. The ROS levels generated in response to silver nanoparticles
were significantly higher in treated PC-3 cells than the control. The result
indicates that cell death is mediated by ROS production, which might alter the
cellular redox status, and it is a potential reason for cell death. Apoptosis of
the silver nanoparticle treated PC-3 cells was accompanied by a reduction in the
percentage of cells in G0/G1 phase and an increase in the percentage of G2/M phase
cells, indicating cell cycle arrest at G2/M phase, and transcription factor NF-κB
plays an essential role in inflammation and cancer. The activation of NF-κB in
response to inflammatory cytokine such as TNF-α promotes nuclear migration to
enable DNA-binding activity and facilitate target genes expression. © 2014 Society
for Industrial and Applied Mathematics and American Statistical Association.
AN - rayyan-553781238
AU - Rajathi, K.
AU - Leneeygreen, K. B.
AU - Suja, S.
DO - 10.37285/ijpsn.2021.14.2.5
IS - 2
KW - Apoptosis
Flow cytometry
NF-kB
Prostate cancer
Silver nanoparticle
TNF
lipocortin 5
propidium iodide
silver nanoparticle
antiapoptotic activity
apoptosis
Article
biosynthesis
cancer inhibition
cell growth
cell migration
controlled study
cytotoxicity
DNA binding
DNA content
DNA fragmentation
drug effect
drug mechanism
flow cytometry
fluorescence intensity
G2 phase cell cycle checkpoint
gene translocation
human
human cell
oxidative stress
PC-3 [Human prostate carcinoma] cell line
percentage of cells in G0/G1 phase
percentage of cells in G2/M phase
priority journal
proapoptotic activity
prostate cancer
protein expression
treatment response
Prostatic Neoplasms
Prostate
PY - 2021
SP - 5396-5405
ST - Mechanism underlying the inhibitory effect of biosynthesized silver
nanoparticle on TNF-α induced NF-κB nuclear translocation in prostate cancer cells
T2 - International Journal of Pharmaceutical Sciences and Nanotechnology
TI - Mechanism underlying the inhibitory effect of biosynthesized silver
nanoparticle on TNF-α induced NF-κB nuclear translocation in prostate cancer cells
85108918316&doi=10.37285%2fijpsn.2021.14.2.5&partnerID=40&md5=a24089d82a59c60082625
9c61bac00be
VL - 14
ID - 9185
ER -
TY - JOUR
AB - Objective Delonix regia(L.) (Caesalpiniaceae) is one of the plants that have
been used for centuries to prevent cancer.D. regiahas been described as a strong
anti-inflammatory, antibacterial, and analgesic. Also, the cytotoxicity of this
plant has been proved due to its flavonoid compounds. Besides, silver (Ag)
nanoparticles have been proved to be promising in cancer treatment. Methods The
synergistic effect of medicinal plant extracts with silver antibacterial
nanoparticles on cancer cell inhibition was investigated. Cytotoxic effect ofD.
regiaextract with silver NPs on MCF-7 and Panc-1 cancer cells was investigated by
cell viability analysis, morphological changes, apoptosis induction, and TUNEL
assay. Results Results showed the cells treated withD. regiaextract containing
AgNPs exhibited a considerable reduction in viability percent in comparison with
control cells. Analysis of MTT demonstrated that the IC(50)value ofD. regiaextract
and AgNPs on both cell lines observed in 0.5 mg/mL for 24 h. The results
demonstrated that the maximum inhibition of both cell lines growth was observed in
1.5 mg/L and 0.2 mg/L in the presence withD. regiaand AgNPs, respectively. Also,
morphological characteristics of cells indicated thatD. regiaextract and silver NPs
induce cell death by apoptosis and it is confirmed by TUNEL assay. Conclusion
Interestingly,D. regiaextract and Ag NPs had no significant cytotoxicity against
normal cells. It can be concluded thatDelonix regiaplant and silver nanoparticles
have an inhibitory potential on two cancer cell lines, but in the use of synergism
ofD. regiaextract and AgNPs, the induction of apoptosis in MCF-7 and Panc-1 was
increased. Interestingly, in normal cells, no significant negative effect was
observed as control.
AN - rayyan-553781241
AU - Rajiri, M. S.
AU - Aminsalehi, M.
AU - Shahbandeh, M.
AU - Maleki, A.
AU - Jonoubi, P.
AU - Rad, A. C.
DO - 10.1007/s13530-020-00067-1
IS - 1
PY - 2021
SN - 2005-9752 2233-7784
SP - 45-56
ST - Anticancer and therapeutic potential ofDelonix regiaextract and silver
nanoparticles (AgNPs) against pancreatic (Panc-1) and breast (MCF-7) cancer cell
T2 - TOXICOLOGY AND ENVIRONMENTAL HEALTH SCIENCES
TI - Anticancer and therapeutic potential ofDelonix regiaextract and silver
nanoparticles (AgNPs) against pancreatic (Panc-1) and breast (MCF-7) cancer cell
VL - 13
Y2 - 3
ID - 9188
ER -
TY - JOUR
AB - The response of a murine macrophage cell line exposed to a library of seven
metal and metal oxide nanoparticles was evaluated via High Throughput Screening
(HTS) assay employing luciferase-reporters for ten independent toxicity-related
signaling pathways. Similarities of toxicity response among the nanoparticles were
identified via Self-Organizing Map (SOM) analysis. This analysis, applied to the
HTS data, quantified the significance of the signaling pathway responses (SPRs) of
the cell population exposed to nanomaterials relative to a population of untreated
cells, using the Strictly Standardized Mean Difference (SSMD). Given the high
dimensionality of the data and relatively small data set, the validity of the SOM
clusters was established via a consensus clustering technique. Analysis of the SPR
signatures revealed two cluster groups corresponding to (i) sublethal pro-
inflammatory responses to Al 2O3, Au, Ag, SiO2 nanoparticles possibly related to
ROS generation, and (ii) lethal genotoxic responses due to exposure to ZnO and Pt
nanoparticles at a concentration range of 25-100 μg/mL at 12 h exposure. In
addition to identifying and visualizing clusters and quantifying similarity
measures, the SOM approach can aid in developing predictive quantitative-structure
relations; however, this would require significantly larger data sets generated
from combinatorial libraries of engineered nanoparticles. © 2011 American Chemical
Society.
AN - rayyan-553781243
AU - Rallo, R.
AU - France, B.
AU - Liu, R.
AU - Nair, S.
AU - George, S.
AU - Damoiseaux, R.
AU - Giralt, F.
AU - Nel, A.
AU - Bradley, K.
AU - Cohen, Y.
DO - 10.1021/es103606x
IS - 4
KW - Animals
Cell Line
Luciferases
Macrophages
Metal Nanoparticles
Mice
Nanostructures
Oxides
Signal Transduction
Murinae
Cell culture
Cell proliferation
Conformal mapping
Metallic compounds
Metals
Nanoparticles
Pigments
Platinum
Population statistics
Signaling
Silicon compounds
Toxicity
Zinc oxide
aluminum oxide
gold
metal nanoparticle
metal oxide nanoparticle
platinum
silicon dioxide
silver
unclassified drug
zinc oxide
luciferase
nanomaterial
oxide
Cell populations
Cell signaling
Combinatorial library
Concentration ranges
Consensus clustering
Data sets
Genotoxic
High dimensionality
High-throughput screening
Murine macrophages
Nano-materials
Pt nanoparticles
Self organizing
Signaling pathways
Similarity measure
Small data set
ZnO
cluster analysis
data set
metal
nanotechnology
toxicity
animal cell
article
audiovisual equipment
cell communication
cell population
controlled study
cytotoxicity
genotoxicity
high throughput screening
macrophage
nonhuman
self organizing map analysis
signal transduction
animal
cell line
chemistry
drug effects
metabolism
mouse
Metal analysis
PY - 2011
SP - 1695-1702
ST - Self-organizing map analysis of toxicity-related cell signaling pathways for
metal and metal oxide nanoparticles
T2 - Environmental Science and Technology
TI - Self-organizing map analysis of toxicity-related cell signaling pathways for
metal and metal oxide nanoparticles
79951650762&doi=10.1021%2fes103606x&partnerID=40&md5=edded015d77f3fd65eaf399d3c22d3
5d
VL - 45
ID - 9190
ER -
TY - JOUR
AB - Methotrexate (MTX) is an antifolate metabolite that is used in the treatment
of various autoimmune diseases, malignancies, and inflammatory disorders. In
addition to the well-characterized side effects such as hepatotoxicity and
myelosuppression, it can also rarely cause a variety of cutaneous manifestations
due to acute toxicity. We are presenting case series of three patients of MTX
toxicity. All three cases presented with acute ulceration and pain over the
psoriatic plaques in addition to mucosal involvement. They were all given
injectable folinic acid. Two out of the three patients died and one of them
recovered. Although low-dose MTX appears to be relatively safe, acute MTX toxicity
is a life-threatening emergency that can occur for which greater awareness of this
condition is needed for its prevention, early diagnosis, and management. © 2022
Journal of the Egyptian Women's Dermatologic Society.
AN - rayyan-553781245
AU - Ramesh, H.
AU - Kanathur, S.
AU - Loganathan, E.
AU - Somashekhar, S.
DO - 10.4103/jewd.jewd_47_21
IS - 2
KW - methotrexate toxicity
overdosing
psoriasis
antibiotic agent
folinic acid
methotrexate
sulfadiazine silver
acute toxicity
adult
Article
bleeding
blood cell count
case report
clinical article
erosion
fever
human
jaundice
low drug dose
male
middle aged
mouth lesion
septic shock
skin care
skin examination
thrombocyte transfusion
ulcer
ulcer healing
Psoriasis
Methotrexate
PY - 2022
SP - 137-140
ST - Acute methotrexate toxicity in patients with psoriasis: case series
T2 - Journal of the Egyptian Women's Dermatologic Society
TI - Acute methotrexate toxicity in patients with psoriasis: case series
85130867339&doi=10.4103%2fjewd.jewd_47_21&partnerID=40&md5=4d1f75f1eb8881b9f0315ae2
7b15af54
VL - 19
ID - 9192
ER -
TY - JOUR
AB - Ten materials used as root canal sealers were evaluated experimentally. These
materials were (1) zinc oxide and eugenol, (2) AH-26, (3) Diaket, (4) Proco-Sol
radiopaque silver cement, (5) Proco-Sol nonstaining root canal cement, (6) Kerr
sealer, (7) Kloroperka, (8) N2, (9) N2 Medical, and (10) Mynol root canal sealer.
Four methods of testing were utilized, as follows: 1. A. Animal study, in which the
subcutaneous connective tissue of the rat was observed for inflammatory response to
implants of the materials tested. 2. B. Tissue-culture toxicity study in which
varying dilutions of the test materials were placed on tissue cultures of HeLa
cells and the degree of toxicity noted. 3. C. A bacteriologic study wherein the
degree of growth inhibition of the test materials to known organisms was measured.
4. D. A selected-material animal study in which four of the test materials were
placed in the conjunctival sac of the eye of the rabbit. This structure was then
observed for degree of inflammation. The materials were found to be fairly
consistent from study to study. Some variables were noted, but for the most part
the materials exhibited a fairly uniform response within each test group when all
methods were compared. © 1964.
AN - rayyan-553781247
AU - Rappaport, H. M.
AU - Lilly, G. E.
AU - Kapsimalis, P.
DO - 10.1016/0030-4220(64)90480-3
IS - 6
KW - Bacteria
Dental Materials
Inflammation
Pathology
Pharmacology
Rabbits
Rats
Research
Root Canal Therapy
Toxicology
Zinc Oxide-Eugenol Cement
BACTERIA
DENTAL MATERIALS
EXPERIMENTAL LAB STUDY
INFLAMMATION
PATHOLOGY
PHARMACOLOGY
RABBITS
RATS
ROOT CANAL THERAPY
TOXICOLOGIC REPORT
ZINC OXIDE-EUGENOL CEMENT
biomedical and dental materials
zinc oxide eugenol
article
bacterium
endodontics
inflammation
pathology
pharmacology
rabbit
rat
research
toxicology
PY - 1964
SP - 785-802
ST - Toxicity of endodontic filling materials
T2 - Oral Surgery, Oral Medicine, Oral Pathology
TI - Toxicity of endodontic filling materials
0008579430&doi=10.1016%2f0030-4220%2864%2990480-
3&partnerID=40&md5=646205536a602a51ebb660e33d7b2b99
VL - 18
ID - 9194
ER -
TY - JOUR
AB - Hepatocellular carcinoma is the most ordinary type of liver cancer and most
commonly appears in a patient with chronic viral hepatitis and with cirrhosis.
Lipopolysaccharide motivates the hepatocyte cells and increases the production
inducible cytokine with the high production of nitric oxide and reactive oxygen
species. In this study, the rate change iNOS searched by adding lipopolysaccharide
and the effect of inhibiting this inflammatory enzyme by L-NAME and combined effect
of this in HepG2 cancer cells was assessed. Salmonella enteritidis
lipopolysaccharide is extracted by methanol-chloroform method and silver stained
SDS-PAGE electrophoresis bands. Four treatment groups of Human liver hepatocellular
carcinoma cell lines stimulated with 100ng/ml lipopolysaccharide, 500 mu M L-NAME
as inhibitor and were incubated for 12 and 24 hours. Variables including both
increase and decrease inflammatory factor, iNOS, was assayed. The obtained results
showed that initial activity of iNOS in not stimulated in HepG2 cell, 0.902U/ml
after stimulation with lipopolysaccharide for 12 and 24hours (0.491U/ml) and
(0.433U/ml) decreased and the effect of this inhibitor was also studied. The data
showed that reducing iNOS in Human liver hepatocellular carcinoma cell lines
correlated with the cell density and duration of incubation with LPS. Inhibition of
enzyme associated with inflammation, with inhibitor substances; L-NAME, observed as
well as lipopolysaccharide. We can potentially design drugs to treat a variety
disease and cancer to use.
AN - rayyan-553781248
AU - Rastegar, H.
AU - Mirzaei, A.
AU - Ashtiani, H. R. A.
AU - Hedayati, M.
AU - Ahmadi, S.
IS - 1
KW - Humanities
Humanism
Humans
Nitric Oxide Synthase
PY - 2012
SN - 0973-7510 2581-690X
SP - 39-45
ST - The Effect of Extracted Bacterial Salmonella enteritidis Lipopolysaccharide
on Inducible Nitric Oxide Synthase in Human Liver Hepatocellular Carcinoma Cell
Lines in Induction and Inhibition Conditions
T2 - JOURNAL OF PURE AND APPLIED MICROBIOLOGY
TI - The Effect of Extracted Bacterial Salmonella enteritidis Lipopolysaccharide
on Inducible Nitric Oxide Synthase in Human Liver Hepatocellular Carcinoma Cell
Lines in Induction and Inhibition Conditions
VL - 6
Y2 - 3
ID - 9195
ER -
TY - JOUR
AB - Bioftlm formation is one of the main obstacles that occur during in vivo
implantation, which compromises the implant functionality and patients' health.
This is the inspiration for the development of novel implant materials that contain
broad-spectrum antimicrobial activity, including antibacterial and antifungal, and
enable the local release of antimicrobial agents. Here, multifunctional calcium
phosphate-ionic liquid (IL) materials, possessing antimicrobial and
repair/regeneration features plus injectability, are proposed as implants in
minimally invasive surgery. This approach was based on the loading of 1-alkyl-3-
alkylimidazolium chloride ionic liquids (ILs) (C(n)MImCl (n = 4, 10, 16) and (C-10)
(2)MImCl) during the in situ sol-gel synthesis of calcium phosphates (CaP) and
study of their effects on CaP crystallization and biological properties. Physical,
morphological, and biological investigations were performed to evaluate the
bionanocomposites' properties. The IL N-alkyl chain length influenced the
crystallization of CaP and, consequently, the biological properties, which afforded
bionanocomposites (when loaded with C(16)MImCl or (C-10)(2)MImCl) that, (i) inhibit
both in vitro bacterial and fungal growth; (ii) reduce the in vitro inflammatory
response; (iii) induce osteogenic differentiation in the basal medium of human
mesenchymal stem cells; and (iv) are injectable. This will enable the design of
multifunctional injectable implants with antimicrobial, anti-inflammatory, and
regenerative properties to be used in minimally invasive surgery of bone and
maxillofacial defects.
AN - rayyan-553781249
AU - Raucci, M. G.
AU - Fasolino, I.
AU - Pastore, S. G.
AU - Soriente, A.
AU - Capeletti, L. B.
AU - Dessuy, M. B.
AU - Giannini, C.
AU - Schrekker, H. S.
AU - Ambrosio, L.
DO - 10.1021/acsami.8b12696
IS - 49
KW - Calcium Phosphates
PY - 2018
SN - 1944-8244
SP - 42766-42776
ST - Antimicrobial Imidazolium Ionic Liquids for the Development of Minimal
Invasive Calcium Phosphate-Based Bionanocomposites
TI - Antimicrobial Imidazolium Ionic Liquids for the Development of Minimal
Invasive Calcium Phosphate-Based Bionanocomposites
VL - 10
Y2 - 12 y3 - 12
ID - 9196
ER -
TY - JOUR
AB - Rheumatoid arthritis (RA) is a progressive, inflammatory, autoimmune,
symmetrical polyarticular arthritis. It is characterized by synovial infiltration
and activation of several types of immune cells, culminating in their apoptosis and
antibody generation against "altered" autoantigens. β2-microglobulin (β2m)-
associated heavy chains (HCs) of HLA antigens, also known as closed conformers
(Face-1), undergo "alteration" during activation of immune cells, resulting in β2m-
free structural variants, including monomeric open conformers (Face-2) that are
capable of dimerizing as either homodimers (Face-3) or as heterodimers (Face-4).
β2m-free HCs uncover the cryptic epitopes that can elicit antibodies (Abs). We
report here the levels of IgM and IgG Abs against both β2m and HCs of HLA-E, HLA-F,
and HLA-G in 74 RA patients receiving immunosuppressive drugs. Anti-β2m IgM was
present in 20 of 74 patients, whereas anti-β2m IgG was found in only 8 patients.
Abs against β2m would be expected if Abs were generated against β2m-associated HLA
HCs. The majority of patients were devoid of either anti-β2m IgM or IgG but had Abs
against HCs of different HLA-Ib molecules. The paucity of anti-β2m Abs in this
cohort of patients suggests that Abs were developed against β2m-free HLA HCs, such
as Face-2, Face-3, and Face-4. While 63 of 68 patients had IgG Abs against anti-
HLA-F HCs, 36 and 50 patients showed IgG Ab reactivity against HLA-E and anti-HLA-G
HCs, respectively. Evidently, anti-HLA-F HC Abs are the most predominant anti-HLA-
Ib HC IgG Abs in RA patients. The incidence and intensity of Abs against HLA-E,
HLA-F, and HLA-G in the normal control group were much higher than those observed
in RA patients. Evidently, the lower level of Abs in RA patients points to the
impact of the immunosuppressive drugs on these patients. These results underscore
the need for further studies to unravel the nature of HLA-F variants on activated
immune cells and synoviocytes of RA patients.
AN - rayyan-553782418
AU - Ravindranath, M. H.
AU - Ravindranath, N. M.
AU - Amato-Menker, C. J.
AU - El Hilali, F.
AU - Selvan, S. R.
AU - Filippone, E. J.
AU - Morales-Buenrostro, L. E.
DO - 10.3390/antib12020026
IS - 2
J2 - Antibodies (Basel)
KW - HLA-G Antigens
Antibodies, Heterophile
LA - eng
N1 - Department of Hematology and Oncology, Children's Hospital, Los Angeles, CA
90027, USA.; Emeritus Research Scientist, Terasaki Foundation Laboratory, Santa
Monica, CA 90064, USA.; Norris Dental Science Center, Herman Ostrow School of
Dentistry, University of Southern California, Los Angeles, CA 90089, USA.;
Department of Microbiology, Immunology, and Cell Biology, School of Medicine, West
Virginia University, Morgantown, WV 26506, USA.; Medico-Surgical, Biomedicine and
Infectiology Research Laboratory, The Faculty of Medicine and Pharmacy of Laayoune
& Agadir, Ibnou Zohr University, Agadir 80000, Morocco.; Division of Immunology and
Hematology Devices, OHT 7: Office of In Vitro Diagnostics, Office of Product
Evaluation and Quality, Center for Devices and Radiological Health, Food and Drug
Administration (FDA), Silver Spring, MD 20993, USA.; Division of Nephrology,
Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University,
Philadelphia, PA 19145, USA.; Department of Nephrology and Mineral Metabolism,
Institute of Nacional Medical Sciences and Nutrition Salvador Zubirán, Vasco de
Quiroga 15, Sección XVI, Mexico City 14000, Mexico.
PY - 2023
ST - Antibodies for β2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and
HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease
Progression in Rheumatoid Arthritis Patients under Treatment
T2 - Antibodies (Basel, Switzerland)
TI - Antibodies for β2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and
HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease
Progression in Rheumatoid Arthritis Patients under Treatment
VL - 12
Y2 - 3 y3 - 31
ID - 10325
ER -
TY - COMP
AB - OBJECTIVES: The "gold standard" in surgical treatment of stress urinary
incontinence (SUI) is sling operation with polypropylene tape appliance under the
mid urethra. There are two types of polypropylene tape which are the most popular
nowadays. These two tapes are differently knitted so they have different
biomechanical features. The TVT tape is monofilament, rarely knitted and highly
elastic but the IVS mesh is multifilament, densly knitted and has only little
possibility to stretch. The aim of our study was to assess the tissue reaction to
the mono-(TVT) and multifilament (IVS) tapes. MATERIAL AND METHODS: The 10 mm x 10
mm pieces of TVT and IVS tapes were implanted inlay the fascia of musculus
abdominis rectus of 14 rat females (2 groups of 7 animals). The tapes with the
margin of surrounding fascia were taken off after 6 weeks of healing. All samples
were fixed in 10% Formaldehyde in phosphate buffered saline and embedded in
paraffin. Four micron tissue sections were stained with hematoxylin and eosin, the
reticulin silver impregnation stain according to Gomori (for collagen type III) and
periodic acid Schiff and alcian blue (for proteoglycan). RESULTS: In all sections
filaments visible as elipsoids were surrounded by resorptive granulation with large
multinucleated giant cells like around "foreign body". The diameter of
monofilaments was about 150 microns. The connective tissue in the vicinity of mesh
was rich of inflammatory cells like histiocytes, lymphocytes, a few
polymorphonuclear leucocytes as well as adipocytes and fibroblasts. The large
multinucleated giant cells adjacent to monofilaments were relatively bigger than
these cells around multifilaments. Moreover, this granulation tissue has a lot of
new blood vessels and collagenous fibrous tissue. The multifilaments were about 40
microns in diameter. The inflammatory granulation infiltrated aggregates of
multifilaments. This tissue had only few inflammatory cells in comparison to tissue
around monofilaments. The large multinucleated giant cells apposed to the mesh were
small and collagen created thicker, more compacted bundles. CONCLUSION: The
multifilament polypropylene tape induces weaker inflammatory tissue reaction than
monofilament mesh. The thicker and more compacted collagen bundles are created
around multifilaments so the natural tensile strength of the surrounding tissue is
probably higher.
AN - rayyan-553782309
AU - Rechberger, T.
AU - Wróbel, A.
AU - Adamiak, A.
AU - Skomra, D.
AU - Korobowicz, E.
AU - Tomaszewski, J.
AU - Czuczwar, M.
AU - Skorupski, P.
CY - Poland
ET - 9
J2 - Ginekol Pol
KW - Animals
Biocompatible Materials/standards
Female
Implants, Experimental/*standards
Polypropylenes/*standards
Rats
Surgical Mesh/*standards
Time Factors
Treatment Outcome
Ultrasonography
Urinary Incontinence, Stress/*diagnostic imaging/pathology/*surgery
Urinary Incontinence
Urinary Incontinence, Stress
ADP Ribose Transferases
LA - pol
N1 - II Katedry i Kliniki Ginekologii AM w Lublinie.
PY - 2003
SN - 0017-0011 (Print)
SP - 1008-13
ST - [Tissue reaction to polypropylene mono-or multi-filament tapes used in
surgical techniques of stress urinary incontinence treatment]
T2 - Ginekologia polska
TI - [Tissue reaction to polypropylene mono-or multi-filament tapes used in
surgical techniques of stress urinary incontinence treatment]
VL - 74
Y2 - 9
ID - 10217
ER -
TY - JOUR
AB - The clinical features Of the maternal syndrome of pre-eclampsia can be
explained by generalised maternal endothelial cell dysfunction, which is a part of
a more global maternal systemic inflammatory response. There is growing evidence
that these effects are associated with the shedding of cellular debris, including
syncytiotrophoblast microparticles (STBM), cell-free DNA and mRNA, from the surface
of the placenta (syncytiotrophoblast) into the maternal circulation. The increased
shedding of this debris seen in pre-eclampsia is believed to be caused by placental
ischaemia, reperfusion and oxidative stress. This, labour and subsequent placental
study was carried out to determine whether uterine contractions during labour and
subsequent placental separation lead to an acute increase in the increase of
placental debt is into the maternal circulation. To assess the effects of labour,
samples were tal(en from 10 normal pregnant (NP) and 10 pre-eclamptic (PF) women at
varied time points. Similarly to assess the effects of placental delivery, plasma
samples were taken From 10 Nil and 10 I'll women Undergoing elective caesarean
section. There was a significant increase in the shedding of STBM in pre-eclampsia
which was not seen in normal pregnancy and there was a small rise in STBM levels at
placental separation in both normal pregnant and pre-eclamptic women undergoing
caesarean section, but the differences were not significant. However, levels of
placental cell-free corticotrophin releasing hormone mRNA were significantly
increased ill labour in both normal pregnancy and pre-eclampsia and were still high
24 h after delivery in the pre-eclamptic women. There was no significant increase
in fetal or total DNA in labour, but the overall levels of total DNA (maternal and
fetal) was increased ill labour In pre-eclampsia compared to normal labour. The
enhanced shedding of STBM and CRH mRNA in pre-eclampsia labour may have a role in
cases of postpartum worsening of pre-eclampsia. (C) 2008 Elsevier Ltd. All rights
reserved.
AN - rayyan-553781255
AU - Reddy, A.
AU - Zhong, X. Y.
AU - Rusterholz, C.
AU - Hahn, S.
AU - Holzgreve, W.
AU - Redman, C. W. G.
AU - Sargent, I. L.
DO - 10.1016/j.placenta.2008.08.018
IS - 11
KW - Pregnancy
Pre-Eclampsia
PY - 2008
SN - 0143-4004 1532-3102
SP - 942-949
ST - The Effect of Labour and Placental Separation on the Shedding of
Syncytiotrophoblast Microparticles, Cell-free DNA and mRNA in Normal Pregnancy and
Pre-eclampsia
T2 - PLACENTA
TI - The Effect of Labour and Placental Separation on the Shedding of
Syncytiotrophoblast Microparticles, Cell-free DNA and mRNA in Normal Pregnancy and
Pre-eclampsia
VL - 29
Y2 - 11
ID - 9201
ER -
TY - JOUR
AB - Production of nanoscale materials often requires the use of toxic chemicals
and complex synthetic procedures. A new scaffold has been explored for in situ
synthesis of nanomaterials that utilizes natural biological systems in the form of
plants, bacteria, fungi, algae and redox-imbalanced mammalian cells and systems.
The latter approach has become popular in recent years and has shown some promising
results in bioimaging of cancer, as well as inflammatory and neurodegenerative
maladies. Biosynthesis of nanoclusters in redox-imbalanced mammalian cells is
facile, cost-effective and environmentally friendly with higher biocompatibility
and target specificity and lower adverse effects than traditional synthetic
approaches. Herein, we describe recent advances in mammalian green in situ
biosynthesis for biomedical applications, especially in cancer and
neurodegenerative disease theranostics.
AN - rayyan-553781256
AU - Rehman, F. U.
AU - Jiang, H.
AU - Selke, M.
AU - Wang, X. M.
DO - 10.1039/c8tb01955j
IS - 41
PY - 2018
SN - 2050-750X 2050-7518
SP - 6501-6514
ST - Mammalian cells: a unique scaffold for in situ biosynthesis of metallic
nanomaterials and biomedical applications
TI - Mammalian cells: a unique scaffold for in situ biosynthesis of metallic
nanomaterials and biomedical applications
VL - 6
Y2 - 11 y3 - 7
ID - 9202
ER -
TY - JOUR
AB - Cardiac mast cells proliferate in cardiovascular diseases. In myocardial
ischemia, mast cell mediators contribute to coronary vasoconstriction, arrhythmias,
leukocyte recruitment, and tissue injury and repair. Arrhythmic dysfunction,
coronary vasoconstriction, and contractile failure are also characteristic of
cardiac anaphylaxis. In coronary atherosclerosis, mast cell mediators facilitate
cholesterol accumulation and plaque destabilization. In cardiac failure, mast cell
chymase causes myocyte apoptosis and fibroblast proliferation, leading to
ventricular dysfunction. Chymase and tryptase also contribute to fibrosis in
cardiomyopathies and myocarditis. In addition, mast cell tumor necrosis factor-
alpha promotes myocardial remodeling. Cardiac remodeling and hypertrophy in end-
stage hypertension are also induced by mast cell mediators and proteases. We
recently discovered that cardiac mast cells contain and release renin, which
initiates local angiotensin formation. Angiotensin causes coronary
vasoconstriction, arrhythmias, fibrosis, apoptosis, and endothelin release, all
demonstrated mechanisms of mast-cell-associated cardiac disease. The effects of
angiotensin are further amplified by the release of norepinephrine from cardiac
sympathetic nerves. Our discovery of renin in cardiac mast cells and its release in
pathophysiological conditions uncovers an important new pathway in the development
of mast-cell-associated heart diseases. Several steps in this novel pathway may
constitute future therapeutic targets.
AN - rayyan-553781257
AU - Reid, A. C.
AU - Silver, R. B.
AU - Levi, R.
DO - 10.1111/j.1600-065X.2007.00514.x
KW - Mast Cells
PY - 2007
SN - 0105-2896 1600-065X
SP - 123-140
ST - Renin: at the heart of the mast cell
T2 - IMMUNOLOGICAL REVIEWS
TI - Renin: at the heart of the mast cell
VL - 217
Y2 - 6
ID - 9203
ER -
TY - JOUR
AB - Background: It is well known that most neurodegenerative diseases are
associated with microglia-mediated inflammation. Our previous research demonstrates
that the CD40 signaling is critically involved in microglia-related immune
responses in the brain. For example, it is well known that the activation of the
signal transducer and activator of transcription (STAT) signaling pathway plays a
central role in interferon-gamma (IFN-gamma)-induced microglial CD40 expression. We
and others have previously reported that microglial CD40 expression is
significantly induced by IFN-gamma and amyloid-beta (A beta) peptide. Recent
studies have shown that certain flavonoids possess antiinflammatory and
neuroprotective properties distinct from their well-known anti-oxidant effects. In
particular, flavonoids, apigenin and luteolin have been found to be effective CD40
immunomodulators. Methods: Cultured microglia, both N9 and primary derived lines,
were treated with flavonoids in the presence of IFN-gamma and/or CD40 ligation to
assess any anti-inflammatory effects and/or mechanisms. CD40 expression on
microglia was analyzed by fluorescence activated cell sorting (FACS). Anti-
inflammatory effects and mechanisms were confirmed by ELISA for interlekin-6 (IL-6)
and TNF-alpha, lactate dehydrogenase (LDH) assay, and STAT1 Western blotting.
Results: Apigenin and luteolin concentration-dependently suppressed IFN-gamma-
induced CD40 expression. Apigenin and luteolin also suppressed microglial TNF-alpha
and IL-6 production stimulated by IFN-gamma challenge in the presence of CD40
ligation. In addition, apigenin and luteolin markedly inhibited IFN-gamma- induced
phosphorylation of STAT1 with little impact on cell survival. Conclusion: Our
findings provide further support for apigenin and luteolin's anti-inflammatory
effects and suggest that these flavonoids may have neuroprotective/disease-
modifying properties in various neurodegenerative disorders, including Alzheimer's
disease (AD).
AN - rayyan-553781258
AU - Rezai-Zadeh, K.
AU - Ehrhart, J.
AU - Bai, Y.
AU - Sanberg, P. R.
AU - Bickford, P.
AU - Tan, J.
AU - Shytle, R. D.
DO - 10.1186/1742-2094-5-41
PY - 2008
SN - 1742-2094
ST - Apigenin and luteolin modulate microglial activation via inhibition of STAT1-
induced CD40 expression
T2 - JOURNAL OF NEUROINFLAMMATION
TI - Apigenin and luteolin modulate microglial activation via inhibition of STAT1-
induced CD40 expression
VL - 5
Y2 - 9 y3 - 25
ID - 9204
ER -
TY - JOUR
AB - Mast cells (MC) are immune cells located next to the intestinal epithelium
with regulatory function in maintaining the homeostasis of the mucosal barrier. We
have investigated MC activities in colon inflammation and cancer in mice either
wild-type (WT) or MC-deficient (Kit(W-sh)) reconstituted or not with bone marrow-
derived MCs. Colitis was chemically induced with dextran sodium sulfate (DSS).
Tumors were induced by administering azoxymethane (AOM) intraperitoneally before
DSS. Following DSS withdrawal, Kit(W-sh) mice showed reduced weight gain and
impaired tissue repair compared with their WT littermates or Kit(W-sh) mice
reconstituted with bone marrow-derived MCs. MCs were localized in areas of mucosal
healing rather than damaged areas where they degraded IL33, an alarmin released by
epithelial cells during tissue damage. Kit(W-sh) mice reconstituted with MC
deficient for mouse mast cell protease 4 did not restore normal mucosal healing or
reduce efficiently inflammation after DSS withdrawal. In contrast with MCs
recruited during inflammation-associated wound healing, MCs adjacent to transformed
epithelial cells acquired a protumorigenic profile. In AOM- and DSS-treated WT
mice, high MC density correlated with high-grade carcinomas. In similarly treated
Kit(W-sh) mice, tumors were less extended and displayed lower histologic grade. Our
results indicate that the interaction of MCs with epithelial cells is dependent on
the inflammatory stage, and on the activation of the tissue repair program.
Selective targeting of MCs for prevention or treatment of inflammation-associated
colon cancer should be timely pondered to allow tissue repair at premalignant
stages or to reduce aggressiveness at the tumor stage.
AN - rayyan-553781892
AU - Rigoni, A.
AU - Bongiovanni, L.
AU - Burocchi, A.
AU - Sangaletti, S.
AU - Danelli, L.
AU - Guarnotta, C.
AU - Lewis, A.
AU - Rizzo, A.
AU - Silver, A. R.
AU - Tripodo, C.
AU - Colombo, M. P.
DO - 10.1158/0008-5472.CAN-14-3767
IS - 18
J2 - Cancer Res
KW - Animals
Animals, Congenic
Azoxymethane/toxicity
Carcinoma/chemically induced/*immunology/pathology
Cell Count
Cell Transformation, Neoplastic/immunology
Cells, Cultured
Colitis/chemically induced/*immunology/pathology
Colonic Neoplasms/chemically induced/*immunology/pathology
Dextran Sulfate/toxicity
Epithelial Cells/pathology
Humans
Inflammatory Bowel Diseases/pathology
Interleukin-1 Receptor-Like 1 Protein
Interleukin-33/physiology
Intestinal Mucosa/*physiology
Mast Cells/*physiology/transplantation
Mice
Mice, Inbred C57BL
Mice, Knockout
Models, Biological
Proto-Oncogene Proteins c-kit/deficiency/genetics
Receptors, Interleukin/physiology
Regeneration/*immunology
Serine Endopeptidases/deficiency
Species Specificity
Specific Pathogen-Free Organisms
Mast Cells
LA - eng
N1 - Molecular Immunology Unit, Department of Experimental Oncology and Molecular
Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.; Tumor
Immunology Unit, Department of Health Sciences, University of Palermo, Palermo,
Italy.; Molecular Immunology Unit, Department of Experimental Oncology and
Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.;
Molecular Immunology Unit, Department of Experimental Oncology and Molecular
Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.; Inserm
UMRS-1149, Paris; Université Paris Diderot, Sorbonne 7 Paris Cite; Laboratoire
d'excellence INFLAMEX, Paris, France.; Tumor Immunology Unit, Department of Health
Sciences, University of Palermo, Palermo, Italy.; Colorectal Cancer Genetics,
Centre for Digestive Diseases, Blizard Institute, Barts and the London School of
Medicine and Dentistry, Whitechapel, London, United Kingdom.; Human Pathology
Section, Ospedali Riuniti Villa Sofia-Cervello, Palermo, Italy.; Colorectal Cancer
Genetics, Centre for Digestive Diseases, Blizard Institute, Barts and the London
School of Medicine and Dentistry, Whitechapel, London, United Kingdom.; Tumor
Immunology Unit, Department of Health Sciences, University of Palermo, Palermo,
Italy.; Molecular Immunology Unit, Department of Experimental Oncology and
Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
mario.colombo@istitutotumori.mi.it.
PY - 2015
SP - 3760-70
ST - Mast Cells Infiltrating Inflamed or Transformed Gut Alternatively Sustain
Mucosal Healing or Tumor Growth
T2 - Cancer research
TI - Mast Cells Infiltrating Inflamed or Transformed Gut Alternatively Sustain
Mucosal Healing or Tumor Growth
VL - 75
Y2 - 9 y3 - 15
ID - 9815
ER -
TY - JOUR
AB - Objectives: Infliximab, an anti-tumor necrosis factor (TNF) monoclonal
antibody, might exert some of its long-term therapeutic effects in Crohn's disease
(CD) by interacting directly with cells of the immune system such as monocytes and
T lymphocytes via membrane TNF and by inducing apoptosis. Accordingly, the effects
of infliximab on monocyte apoptosis and down-regulation of proinflammatory
cytokines (reverse signaling) were assessed. Methods: To assess apoptosis,
monocytes from healthy individuals (controls) and CD patients were incubated in the
presence or absence of infliximab or the apoptotic agent gliotoxin for 24 hours.
Annexin V staining and the terminal deoxynucleotidyl transferase (TdT)-mediated
dUTP-FITC nick end labeling assay were used to measure early and late apoptosis. To
measure the effects of infliximab on reverse signaling, monocytes from healthy
individuals pretreated in vitro with infliximab were stimulated with
lipopolysaccharide or staphylococcal enterotoxin A, and the induction of the
proinflammatory cytokines, TNF-alpha, interleukin (IL)-1beta, IL-6, and IL-8 was
measured by reverse transcription polymerase chain reaction. The effect of in vivo
infliximab treatment of monocytes was similarly determined by comparing the
responses of monocytes from CD patients before and immediately after infliximab
infusion. Results: Infliximab did not induce apoptosis of monocytes from either
healthy individuals or CD patients but rather stabilized them. However, monocytes
from healthy individuals treated with infliximab in vitro, or from CD patients
infused with infliximab, produced significantly less TNF and other proinflammatory
cytokines when stimulated with the bacterial products lipopolysaccharide and
staphylococcal enterotoxin A. Conclusions: Apoptosis of monocytes is not
responsible for the therapeutic effects of infliximab. However, some of the
therapeutic effects of infliximab may be caused by its ability to down-regulate
proinflammatory cytokines production by monocytes exposed to bacterial antigens.
AN - rayyan-553781259
AU - Ringheanu, M.
AU - Daum, F.
AU - Markowitz, J.
AU - Levine, J.
AU - Katz, S.
AU - Lin, X. Y.
AU - Silver, J.
DO - 10.1097/00054725-200411000-00015
IS - 6
KW - Crohn Disease
Monocytes
Apoptosis
PY - 2004
SN - 1078-0998 1536-4844
SP - 801-810
ST - Effects of infliximab on apoptosis and reverse signaling of monocytes from
healthy individuals and patients with Crohn's disease
T2 - INFLAMMATORY BOWEL DISEASES
TI - Effects of infliximab on apoptosis and reverse signaling of monocytes from
healthy individuals and patients with Crohn's disease
VL - 10
Y2 - 11
ID - 9205
ER -
TY - JOUR
AB - In our current study, we synthesized silver nanoparticles (AgNPs) from an
aqueous seed extract of Nigella sativa. The seed extract contains phytochemical
compounds including phenols, terpenoids, and flavonoids that may act as reducing
agents and are able to convert metal ions to metal nanoparticles. The formation of
synthesized AgNPs was characterized using UV-visible spectroscopy, Fourier
transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and
energy dispersive analysis of X-rays (EDX). The efficacy of N-AgNPs against human
breast cancer (MCF-7) cells was tested. The synthesized AgNPs displayed dose-
dependent cytotoxicity (1–200 µg/mL) against MCF-7 cells. Morphological alterations
of the cells also appeared as bright field images. Treatment of synthesized AgNPs
altered the expression of Bax and Bcl-2 (apoptotic proteins) and COX-2
(inflammatory marker) in MCF-7 cells. To our knowledge, this is the first report
demonstrating that N-AgNPs from Nigella sativa can induce apoptosis in MCF-7 cells.
© 2019 by Begell House, Inc.
AN - rayyan-553781261
AU - Rohini, B.
AU - Akther, T.
AU - Waseem, M.
AU - Khan, J.
AU - Kashif, M.
AU - Hemalatha, S.
DO - 10.1615/JEnvironPatholToxicolOncol.2019027318
IS - 2
KW - AgNPs
Apoptosis
Breast cancer
Mitochondria
Breast Neoplasms
Humans
MCF-7 Cells
Metal Nanoparticles
Nigella sativa
Plant Extracts
Seeds
Silver
cyclooxygenase 2
plant extract
protein Bax
protein bcl 2
silver nanoparticle
metal nanoparticle
silver
Article
black cumin
breast cancer
drug cytotoxicity
human
human cell
in vitro study
MCF-7 cell line
priority journal
apoptosis
breast tumor
chemistry
drug effect
plant seed
PY - 2019
SP - 185-194
ST - AgNPs from nigella sativa control breast cancer: An in vitro study
T2 - Journal of Environmental Pathology, Toxicology and Oncology
TI - AgNPs from nigella sativa control breast cancer: An in vitro study
85069809028&doi=10.1615%2fJEnvironPatholToxicolOncol.2019027318&partnerID=40&md5=55
4cd86d970d1e1fd76324c911ef8685
VL - 38
ID - 9207
ER -
TY - JOUR
AB - Despite the fact that the association of Helicobacter pylori with an
increased risk of gastric cancer is well documented, the exact mechanisms of this
association have not been elucidated. Our aim was to shed some light on these
mechanisms by studying the relationship of H. pylori CagA status to gastric cell
proliferation and apoptosis, since both play an important role in gastrointestinal
epithelial cell turnover and carcinogenesis. We studied fifty patients [32 men, 18
women, median age 39.5 years (range 18-67)], referred for upper gastrointestinal
endoscopy, from whom antral biopsies were taken. On biopsy specimens gastritis was
estimated by scoring the severity of inflammatory infiltrate, and the presence of
atrophy and intestinal metaplasia were also noted. The gastric cell proliferation
index (PI) was estimated by AgNOR staining, the epithelial apoptotic index (AI) was
measured by special staining for apoptosis, and CagA status was determined
serologically by immunoblotting the sera of patients against H. pylori antigens.
Thirty-eight (76%) of the 50 patients were H. pylori (positive) and 12 (24%) H.
pylori (negative). Among the 38 H. pylori(+) patients, 28 (73.6%) were CagA(+) and
10 (24.6%) CagA(-). In the H. pylori CagA(+) and CagA(-) groups, the PI values
[median (ranges)] were 5 (4-7) and 3.7 (3.5-5.5), respectively (P < 0.05). In
addition the difference in PI between the H. pylori CagA(+) and H. pylori(-) groups
was highly significant (P < 0.001). Concerning apoptosis, in the H. pylori CagA(+)
and CagA(-) groups, the values for AI were 1 (1-30) and 5.5 (1-35), respectively (P
< 0.05). In addition, the difference in AI between the H. pylori CagA(-) and H.
pylori(-) groups, was significant (P < 0.05). We conclude that H. pylori CagA(+)
strains induce increased gastric cell proliferation, which is not accompanied by a
parallel increase in apoptosis. This might explain the increased risk for gastric
carcinoma that is associated with infection by H. pylori CagA(+) strains.
AN - rayyan-553781262
AU - Rokkas, T.
AU - Ladas, S.
AU - Liatsos, C.
AU - Petridou, E.
AU - Papatheodorou, G.
AU - Theocharis, S.
AU - Karameris, A.
AU - Raptis, S.
DO - 10.1023/A:1026636803101
IS - 3
KW - Apoptosis
CagA Status
Gastric carcinogenesis
Helicobacter pylori infection
Proliferation
adult
aged
apoptosis
article
cancer risk
cell proliferation
clinical article
female
gastritis
Helicobacter pylori
human
male
priority journal
silver staining
stomach biopsy
stomach cancer
stomach carcinogenesis
PY - 1999
SP - 487-493
ST - Relationship of Helicobacter pylori CagA status to gastric cell proliferation
and apoptosis
T2 - Digestive Diseases and Sciences
TI - Relationship of Helicobacter pylori CagA status to gastric cell proliferation
and apoptosis
0032987485&doi=10.1023%2fA
%3a1026636803101&partnerID=40&md5=0e8fde5130c2d7c686dead8dba4e58a3
VL - 44
ID - 9208
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are useful to a wide range of consumer's and
medical products, due to their antimicrobial and anti-inflammatory activities.
AgNPs have been used to prevent the microbial colonization, therefore decreasing
the risk of infection, on implantable devices, tumor prostheses, bone cement and
surgical instruments. However, the putative toxicity of AgNPs to bone cells is
still poorly understood. Therefore, this study aimed to contribute to enlighten the
role of ionic silver release of small sized NPs on the biological outcomes of bone
cells, in particular to what concerns to induction of cytotoxic and genotoxic
effects. To achieve that goal osteoblast-like MG-63 cells were exposed to well
characterized PVP coated AgNPs of two different primary sizes (10 nm and 20 nm) and
evaluated after 24 and 48 h. Our results showed that, the smaller sized AgNPs (10
nm) are more reactive and prone to form large aggregates, being therefore mandatory
to provide a careful characterization of the particles, before the toxicity
assessment. We also demonstrate that for short period exposures (up to 48 h) ionic
silver (from AgNO3) is more toxic than the corresponding dose of AgNP. However,
when assessing longer term exposures by the clonogenic assay, we demonstrated the
inverse effect, the AgNPs turn out being more toxic, completely inhibiting plate
efficiency. Therefore, AgNPs toxicity cannot be attributed to the dissociated Ag+
alone. Also, when comparing size-dependent effects, we demonstrate that AgNP20 were
found to induce a cell cycle arrest at G0/G1 and apoptosis, while AgNP10 did not
induce a cytostatic effect, but rather induced necrosis. Finally, combining the
chemical and toxicological profiles of both AgNP sizes, we hypothesize that the
size dependent AgNP toxicity may be associated in part with the NPs interference
with the cell membranes and consequent uptake/adsorption processes. (C) 2016
Elsevier Ireland Ltd. All rights reserved.
AN - rayyan-553781265
AU - Rosario, F.
AU - Hoet, P.
AU - Santos, C.
AU - Oliveira, H.
DO - 10.1016/j.tox.2016.08.020
KW - Cell Death
PY - 2016
SN - 0300-483X
SP - 103-115
ST - Death and cell cycle progression are differently conditioned by the AgNP size
in osteoblast-like cells
T2 - TOXICOLOGY
TI - Death and cell cycle progression are differently conditioned by the AgNP size
VL - 368
Y2 - 8 y3 - 10
ID - 9211
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are useful to a wide range of consumer's and
medical products, due to their antimicrobial and anti-inflammatory activities.
AgNPs have been used to prevent the microbial colonization, therefore decreasing
the risk of infection, on implantable devices, tumor prostheses, bone cement and
surgical instruments. However, the putative toxicity of AgNPs to bone cells is
still poorly understood. Therefore, this study aimed to contribute to enlighten the
role of ionic silver release of small sized NPs on the biological outcomes of bone
cells, in particular to what concerns to induction of cytotoxic and genotoxic
effects. To achieve that goal osteoblast-like MG-63 cells were exposed to well
characterized PVP coated AgNPs of two different primary sizes (10nm and 20nm) and
evaluated after 24 and 48h. Our results showed that, the smaller sized AgNPs (10nm)
are more reactive and prone to form large aggregates, being therefore mandatory to
provide a careful characterization of the particles, before the toxicity
assessment. We also demonstrate that for short period exposures (up to 48h) ionic
silver (from AgNO(3)) is more toxic than the corresponding dose of AgNP. However,
when assessing longer term exposures by the clonogenic assay, we demonstrated the
inverse effect, the AgNPs turn out being more toxic, completely inhibiting plate
efficiency. Therefore, AgNPs toxicity cannot be attributed to the dissociated Ag(+)
alone. Also, when comparing size-dependent effects, we demonstrate that AgNP20 were
found to induce a cell cycle arrest at G0/G1 and apoptosis, while AgNP10 did not
induce a cytostatic effect, but rather induced necrosis. Finally, combining the
chemical and toxicological profiles of both AgNP sizes, we hypothesize that the
size dependent AgNP toxicity may be associated in part with the NPs interference
with the cell membranes and consequent uptake/adsorption processes.
AN - rayyan-553781882
AU - Rosário, F.
AU - Hoet, P.
AU - Santos, C.
AU - Oliveira, H.
DO - 10.1016/j.tox.2016.08.020
J2 - Toxicology
KW - Apoptosis/drug effects
Bone and Bones/cytology/drug effects
Cell Cycle/*drug effects
Cell Cycle Checkpoints/drug effects
Cell Line, Tumor
DNA Damage
Humans
Necrosis/chemically induced/pathology
Osteoblasts/cytology/*drug effects
Particle Size
Silver/*toxicity
Cell Death
LA - eng
N1 - CESAM & Department of Biology, University of Aveiro, 3810-193 Aveiro,
Portugal.; Occupational and Environmental Toxicology, KU Leuven, Leuven, Belgium.;
Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo
Alegre, Porto, Portugal. Electronic address: csantos@fc.up.pt.; CESAM & Department
of Biology, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address:
holiveira@ua.pt.
PY - 2016
SP - 103-115
ST - Death and cell cycle progression are differently conditioned by the AgNP size
T2 - Toxicology
TI - Death and cell cycle progression are differently conditioned by the AgNP size
VL - 368
Y2 - 8 y3 - 10
ID - 9806
ER -
TY - JOUR
AB - Glucagon Like Peptide-1 (GLP-1) drugs are currently used to treat type-2
diabetes. Safety concerns for increased risk of pancreatitis and pancreatic ductal
metaplasia have accompanied these drugs. High fat diet (HFD) is a type-2 diabetes
risk factor that may affect the response to GLP-1 drug treatment. The objective of
the present study was to investigate the effects of diet and GLP-1 based drugs on
the exocrine pancreas in mice. Experiments were designed in a mouse model of
insulin resistance created by feeding a HFD or standard diet (STD) for 6 weeks. The
GLP-1 drugs, sitagliptin (SIT) and exenatide (EXE) were administered once daily for
additional 6 weeks in both mice fed HFD or STD. The results showed that body
weight, blood glucose levels, and serum levels of pro-inflammatory cytokines (TNF
alpha, IL-1 beta, and KC) were significantly greater in HFD mice than in STD mice
regardless of GLP-1 drug treatment. The semi-quantitative grading showed that
pancreatic changes were significantly greater in EXE and SIT-treated mice compared
to control and that HFD exacerbated spontaneous exocrine pancreatic changes seen in
saline-treated mice on a standard diet. Exocrine pancreatic changes identified in
this study included acinar cell injury (hypertrophy, autophagy, apoptosis,
necrosis, and atrophy), vascular injury, interstitial edema and inflammation, fat
necrosis, and duct changes. These findings support HFD as a risk factor to
increased susceptibility/severity for acute pancreatitis and indicate that GLP-1
drugs cause pancreatic injury that can be exacerbated in a HFD environment.
Published by Elsevier Inc.
AN - rayyan-553781266
AU - Rouse, R.
AU - Xu, L.
AU - Stewart, S.
AU - Zhang, J.
DO - 10.1016/j.taap.2014.01.021
IS - 2
KW - Mice
PY - 2014
SN - 0041-008X 1096-0333
SP - 104-114
ST - High fat diet and GLP-1 drugs induce pancreatic injury in mice
TI - High fat diet and GLP-1 drugs induce pancreatic injury in mice
VL - 276
Y2 - 4 y3 - 15
ID - 9212
ER -
TY - COMP
AB - Liver microphysiological systems (MPSs) are promising models for predicting
hepatic drug effects. Yet, after a decade since their introduction, MPSs are not
routinely used in drug development due to lack of criteria for ensuring
reproducibility of results. We characterized the feasibility of a liver MPS to
yield reproducible outcomes of experiments assaying drug toxicity, metabolism, and
intracellular accumulation. The ability of the liver MPS to reproduce hepatotoxic
effects was assessed using trovafloxacin, which increased lactate dehydrogenase
(LDH) release and reduced cytochrome P450 3A4 (CYP3A4) activity. These observations
were made in two test sites and with different batches of Kupffer cells. Upon
culturing equivalent hepatocytes in the MPS, spheroids, and sandwich cultures,
differences between culture formats were detected in CYP3A4 activity and albumin
production. Cells in all culture formats exhibited different sensitivities to
hepatotoxicant exposure. Hepatocytes in the MPS were more functionally stable than
those of other culture platforms, as CYP3A4 activity and albumin secretion remained
prominent for greater than 18 days in culture, whereas functional decline occurred
earlier in spheroids (12 days) and sandwich cultures (7 days). The MPS was also
demonstrated to be suitable for metabolism studies, where CYP3A4 activity,
troglitazone metabolites, diclofenac clearance, and intracellular accumulation of
chloroquine were quantified. To ensure reproducibility between studies with the
MPS, the combined use of LDH and CYP3A4 assays were implemented as quality control
metrics. Overall results indicated that the liver MPS can be used reproducibly in
general drug evaluation applications. Study outcomes led to general considerations
and recommendations for using liver MPSs. Study Highlights WHAT IS THE CURRENT
KNOWLEDGE ON THE TOPIC? Microphysiological systems (MPSs) have been designed to
recreate organ- or tissue-specific characteristics of extracellular
microenvironments that enhance the physiological relevance of cells in culture.
Liver MPSs enable long-lasting and stable culture of hepatic cells by culturing
them in three-dimensions and exposing them to fluid flow. WHAT QUESTION DID THIS
STUDY ADDRESS? What is the functional performance relative to other cell culture
platforms and the reproducibility of a liver MPS for assessing drug development and
evaluation questions, such as toxicity, metabolism, and pharmacokinetics? WHAT DOES
THIS STUDY ADD TO OUR KNOWLEDGE? The liver MPS systematically detected the toxicity
of trovafloxacin. When compared with spheroids and sandwich cultures, this system
had a more stable function and different sensitivity to troglitazone, tamoxifen,
and digoxin. Quantifying phase II metabolism of troglitazone and intracellular
accumulation of chloroquine demonstrated the potential use of the liver MPS for
studying drug metabolism and pharmacokinetics. Quality control criteria for
assessing chip function were key for reliably using the liver MPS. HOW MIGHT THIS
CHANGE CLINICAL PHARMACOLOGY OR TRANSLATIONAL SCIENCE? Due to its functional
robustness and physiological relevance (3D culture, cells expose to fluid flow and
co-culture of different cell types), the liver MPS can, in a reproducible manner:
(i) detect inflammatory-induced drug toxicity, as demonstrated with trovafloxacin,
(ii) detect the toxicity of other drugs, such as troglitazone, tamoxifen, and
digoxin, with different effects than those detected in spheroids and sandwich
cultures, (iii) enable studies of hepatic function that rely on prolonged cellular
activity, and (iv) detect phase II metabolites and drug accumulation to potentially
support the interpretation of clinical data. The integration of MPSs in drug
development will be facilitated by careful evaluation of performance and
reproducibility as performed in this study.
AN - rayyan-553782194
AU - Rubiano, A.
AU - Indapurkar, A.
AU - Yokosawa, R.
AU - Miedzik, A.
AU - Rosenzweig, B.
AU - Arefin, A.
AU - Moulin, C. M.
AU - Dame, K.
AU - Hartman, N.
AU - Volpe, D. A.
AU - Matta, M. K.
AU - Hughes, D. J.
AU - Strauss, D. G.
AU - Kostrzewski, T.
AU - Ribeiro, A. J. S.
CY - United States
DO - 10.1111/cts.12969
ET - 3
J2 - Clin Transl Sci
Cytochrome P-450 CYP3A/metabolism
Hepatocytes/drug effects/metabolism
Humans
Lab-On-A-Chip Devices
Liver/cytology/*drug effects/metabolism
Models, Biological
Primary Cell Culture/instrumentation/*methods
Spheroids, Cellular
Toxicity Tests/instrumentation/*methods
Liver
LA - eng
N1 - Center for Drug Evaluation and Research, Office of Translational Sciences,
Office of Clinical Pharmacology, Division of Applied Regulatory Science, US Food
and Drug Administration, Silver Spring, Maryland, USA.; Center for Drug Evaluation
and Research, Office of Translational Sciences, Office of Clinical Pharmacology,
Division of Applied Regulatory Science, US Food and Drug Administration, Silver
Spring, Maryland, USA.; Center for Drug Evaluation and Research, Office of
Translational Sciences, Office of Clinical Pharmacology, Division of Applied
Regulatory Science, US Food and Drug Administration, Silver Spring, Maryland, USA.;
CN Bio Innovations Limited, Cambridge, UK.; Center for Drug Evaluation and
Research, Office of Translational Sciences, Office of Clinical Pharmacology,
Center for Drug Evaluation and Research, Office of Translational Sciences, Office
of Clinical Pharmacology, Division of Applied Regulatory Science, US Food and Drug
Administration, Silver Spring, Maryland, USA.; Center for Drug Evaluation and
Center for Drug Evaluation and Research, Office of Translational Sciences, Office
of Clinical Pharmacology, Division of Applied Regulatory Science, US Food and Drug
Spring, Maryland, USA.; CN Bio Innovations Limited, Cambridge, UK.; Center for Drug
Evaluation and Research, Office of Translational Sciences, Office of Clinical
Pharmacology, Division of Applied Regulatory Science, US Food and Drug
Research, Office of Translational Sciences, Office of Clinical Pharmacology, US
Food and Drug Administration, Silver Spring, Maryland, USA.; CN Bio Innovations
Limited, Cambridge, UK.; Center for Drug Evaluation and Research, Office of
Regulatory Science, US Food and Drug Administration, Silver Spring, Maryland, USA.
PY - 2021
SP - 1049-1061
ST - Characterizing the reproducibility in using a liver microphysiological system
for assaying drug toxicity, metabolism, and accumulation
T2 - Clinical and translational science
TI - Characterizing the reproducibility in using a liver microphysiological system
for assaying drug toxicity, metabolism, and accumulation
VL - 14
Y2 - 5
ID - 10104
ER -
TY - JOUR
AB - A method is described, by which retrograde root filling with a composite
resin can be performed. The cavity design is a slightly concave dissection of the
apical part of the root, which is treated with the bonding agent Gluma followed by
an application of Retroplast. Retroplast is a chemically curable composite
containing silver for radiopacity and aerosil to obtain a suitable consistency.
Endodontically treated teeth with a eugenol-containing root canal sealer did not
affect the strength of the bond between Retroplast and apical dentin. A tight seal
between the composite and the cavity surface was observed by light and SEM
microscopy, and histology of tissue surrounding fillings placed in monkeys revealed
absence of inflammatory cells around the filling and a close contact between
filling and fibroblasts with collagenous fibers. In some cases, cementum and
Sharpey's fibers formed in contact with the filling. Fillings placed in humans
performed successfully in most cases, and the main causes of failure were either
inadequate hemostasis during filling, or root fracture unnoticed by the time of
filling. The retrograde technique promises a new treatment principle, with a root
canal effectively sealed and the periapical ligament restored after apicoectomy.
AN - rayyan-553782313
AU - Rud, J.
AU - Munksgaard, E. C.
AU - Andreasen, J. O.
AU - Rud, V.
AU - Asmussen, E.
DO - 10.1111/j.1600-9657.1991.tb00195.x
IS - 3
J2 - Endod Dent Traumatol
KW - Adolescent
Adult
Aged
Animals
Chlorocebus aethiops
*Composite Resins
*Dental Bonding
Dentin/ultrastructure
Dry Socket/etiology
Female
*Glutaral
Humans
Male
Materials Testing
Middle Aged
*Polymethacrylic Acids
Retrograde Obturation/*methods
*Root Canal Filling Materials
Silver
Tensile Strength
LA - eng
N1 - Department of Dental Materials and Technology, Royal Dental College,
Copenhagen.
PY - 1991
SN - 0109-2502 (Print)
SP - 118-25
ST - Retrograde root filling with composite and a dentin-bonding agent. 1
T2 - Endodontics & dental traumatology
TI - Retrograde root filling with composite and a dentin-bonding agent. 1
VL - 7
Y2 - 6
ID - 10221
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) have been increasingly incorporated into food-
related and hygiene products for their unique antimicrobial and preservative
properties. The consequent oral exposure may then result in unpredicted harmful
effects in the gastrointestinal tract (GIT), which should be considered in the risk
assessment and risk management of these materials. In the present study, the toxic
effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-
relevant cells (Caco-2 cell line as a model of human intestinal cells, and
neutrophils as a model of the intestinal inflammatory response). This study also
evaluated the putative protective action of dietary flavonoids against such harmful
effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced
Caco-2 cell death by apoptosis with concomitant production of nitric oxide,
irrespective of the size. It was also observed that AgNP induced human neutrophil
oxidative burst. Interestingly, some flavonoids, namely quercetin and
quercetagetin, prevented the deleterious effects of AgNP in both cell types.
Overall, the data of the present study provide a first insight into the promising
protective role of flavonoids against the potentially toxic effects of AgNP at the
intestinal level.
AN - rayyan-553781269
AU - Rufino, A. T.
AU - Ramalho, A.
AU - Sousa, A.
AU - de Oliveira, Jmpf
AU - Freitas, P.
AU - Gomez, M. A. G.
AU - Pineiro-Redondo, Y.
AU - Rivas, J.
AU - Carvalho, F.
AU - Fernandes, E.
AU - Freitas, M.
DO - 10.3390/molecules26216610
IS - 21
KW - Flavonoids
PY - 2021
SN - 1420-3049
ST - Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of
T2 - MOLECULES
TI - Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of
VL - 26
Y2 - 11
ID - 9215
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) have been increasingly incorporated into food-
related and hygiene products for their unique antimicrobial and preservative
properties. The consequent oral exposure may then result in unpredicted harmful
effects in the gastrointestinal tract (GIT), which should be considered in the risk
assessment and risk management of these materials. In the present study, the toxic
effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-
relevant cells (Caco-2 cell line as a model of human intestinal cells, and
neutrophils as a model of the intestinal inflammatory response). This study also
evaluated the putative protective action of dietary flavonoids against such harmful
effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced
Caco-2 cell death by apoptosis with concomitant production of nitric oxide,
irrespective of the size. It was also observed that AgNP induced human neutrophil
oxidative burst. Interestingly, some flavonoids, namely quercetin and
quercetagetin, prevented the deleterious effects of AgNP in both cell types.
Overall, the data of the present study provide a first insight into the promising
protective role of flavonoids against the potentially toxic effects of AgNP at the
intestinal level.
AN - rayyan-553781854
AU - Rufino, A. T.
AU - Ramalho, A.
AU - Sousa, A.
AU - de Oliveira, Jmpf
AU - Freitas, P.
AU - Gómez, M. A. G.
AU - Piñeiro-Redondo, Y.
AU - Rivas, J.
AU - Carvalho, F.
AU - Fernandes, E.
AU - Freitas, M.
DO - 10.3390/molecules26216610
IS - 21
J2 - Molecules
KW - Apoptosis/drug effects
Caco-2 Cells
Flavonoids/chemistry/*pharmacology
Humans
Inflammation/*drug therapy/metabolism
Intestinal Mucosa/*drug effects/metabolism
Particle Size
Protective Agents/chemistry/*pharmacology
Flavonoids
LA - eng
N1 - LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical
Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.;
LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences,
Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.; LAQV,
REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty
of Pharmacy, University of Porto, 4050-313 Porto, Portugal.; LAQV, REQUIMTE,
Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of
Pharmacy, University of Porto, 4050-313 Porto, Portugal.; International Iberian
Nanotechnology Laboratory, 4715-330 Braga, Portugal.; Nanotechnology and Magnetism
Lab-NANOMAG, Department of Applied Physics, Universidade de Santiago de Compostela,
15782 Santiago de Compostela, Spain.; Nanotechnology and Magnetism Lab-NANOMAG,
Department of Applied Physics, Universidade de Santiago de Compostela, 15782
Santiago de Compostela, Spain.; Nanotechnology and Magnetism Lab-NANOMAG,
Department of Applied Physics, Universidade de Santiago de Compostela, 15782
Santiago de Compostela, Spain.; UCIBIO, REQUIMTE, Laboratory of Toxicology,
Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-
313 Porto, Portugal.; LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department
of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto,
Portugal.; LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical
Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
PY - 2021
ST - Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of
TI - Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of
VL - 26
Y2 - 10 y3 - 31
ID - 9780
ER -
TY - JOUR
AB - Biodegradable polymers of natural origin are ideal for the development of
processes in tissue engineering due to their immunogenic potential and ability to
interact with living tissues. However, some synthetic polymers have been developed
in recent years for use in tissue engineering, such as Poly-epsilon-caprolactone.
The nanotechnology and the electrospinning process are perceived to produce
biomaterials in the form of nanofibers with diverse unique properties.
Biocompatibility tests of poly-epsilon-caprolactone nanofibers embedded with
hydroxyapatite and alumina nanoparticles manufactured by means of the
electrospinning technique were carried out in Wistar rats to be used as oral
dressings. Hydroxyapatite as a material is used because of its great compatibility,
bioactivity, and osteoconductive properties. The PCL, PCL-HA, PCL-alpha-Al2O3, and
PCL-HA-alpha-Al2O3 nanofibers obtained in the process were characterized by
infrared spectroscopy and scanning electron microscopy. The nanofibers had an
average diameter of (840 +/- 230) nm. The nanofiber implants were placed and tested
at 2, 4, and 6 weeks in the subcutaneous tissue of the rats to give a chronic
inflammatory infiltrate, characteristic foreign body reaction, which decreased
slightly at 6 weeks with the addition of hydroxyapatite and alumina ceramic
particles. The biocompatibility test showed a foreign body reaction that produces a
layer of collagen and fibroblasts. Tissue loss and necrosis were not observed due
to the coating of the material, but a slight decrease in the inflammatory
infiltrate occurred in the last evaluation period, which is indicative of the
beginning of the acceptance of the tested materials by the organism.
AN - rayyan-553781270
AU - Ruiz-Ramirez, L. R.
AU - Alvarez-Ortega, O.
AU - Donohue-Cornejo, A.
AU - Espinosa-Cristobal, L. F.
AU - Farias-Mancilla, J. R.
AU - Martinez-Perez, C. A.
AU - Reyes-Lopez, S. Y.
DO - 10.3390/polym14112130
IS - 11
KW - Poly(A)-Binding Protein II
RNA, Messenger
Poly I-C
Poly A-U
Poly G
Poly T
Polylysine
Rats
Polyglactin 910
PY - 2022
SN - 2073-4360
ST - Poly-epsilon-Caprolactone-Hydroxyapatite-Alumina (PCL-HA-alpha-Al2O3)
Electrospun Nanofibers in Wistar Rats
T2 - POLYMERS
TI - Poly-epsilon-Caprolactone-Hydroxyapatite-Alumina (PCL-HA-alpha-Al2O3)
Electrospun Nanofibers in Wistar Rats
VL - 14
Y2 - 6
ID - 9216
ER -
TY - JOUR
AB - Iron oxide nanoparticles (Fe3O4 NPs) are important for different medical
applications. However, potential toxicity has been reported and several parameters
must still be studied to reach highest therapeutic efficacy with minimal undesired
effects. Inflammation is one of the most reported undesired effects of NP exposure
in a variety of inflammatory models and conflicting data exist regarding whether
Fe3O4 NPs possess pro-or anti-inflammatory activities. The aim of this study was to
determine the direct effect of Fe3O4 NPs on the biology of neutrophil, a key player
cell in inflammation. Freshly isolated human neutrophils were incubated in vitro
with Fe3O4 NPs, and several functions have been studied. Using transmission
electronic microscopy, Fe3O4 NPs were found to be ingested by neutrophils. These
NPs do not induce a respiratory burst by themselves, but they increase the ability
of neutrophils to adhere onto human endothelial cells as well as enhance
phagocytosis. An antibody array approach revealed that Fe3O4 NPs induce the
production of some cytokines, including the chemokine IL-8 (CXCL8), which was
confirmed by ELISA. Fe(3)O(4)NPs were found to delay spontaneous neutrophil
apoptosis regardless of sex of the donor. Using a pharmacological approach, we
demonstrate that Fe3O4 NPs delay apoptosis by a de novo protein synthesis-dependent
mechanism and via different cell signalling pathways. The data indicate that Fe3O4
NPs can alter the biology of human neutrophils and that they possess some pro -
inflammatory effects, particularly based on their capacity to delay apoptosis and
to induce the production of pro-inflammatory cytokines. Therefore, Fe3O4 NPs can
regulate inflammation by targeting human neutrophil functions.
AN - rayyan-553781272
AU - Saafane, A.
AU - Girard, D.
DO - 10.1016/j.cbi.2022.110053
KW - Humanities
Humanism
Humans
Oxalic Acid
Iron
Norisoprenoids
PY - 2022
SN - 0009-2797 1872-7786
ST - Interaction between iron oxide nanoparticles (Fe3O4 NPs) and human
neutrophils: Evidence that Fe3O4 NPs possess some pro-inflammatory activities
T2 - CHEMICO-BIOLOGICAL INTERACTIONS
TI - Interaction between iron oxide nanoparticles (Fe3O4 NPs) and human
neutrophils: Evidence that Fe3O4 NPs possess some pro-inflammatory activities
VL - 365
Y2 - 9 y3 - 25
ID - 9218
ER -
TY - JOUR
AB - Nanotechnology has been gaining more and more momentum lately and the
potential use of nanomaterials such as nanoparticles (NPs) continues to grow in a
variety of activity sectors. Among the NPs, iron oxide nanoparticles (IONs) have
retained an increasing interest from the scientific community and industrials due
to their superparamagnetic properties allowing their use in many fields, including
medicine. However, some undesired effects of IONs and potential risk for human
health are becoming increasingly reported in several studies. Although many in vivo
studies reported that IONs induce immunotoxicity in different animal models, it is
not clear how IONs can alter the biology of primary human immune cells. In this
article, we will review the works that have been done regarding the interaction
between IONs and primary immune cells. This review also outlines the importance of
using primary immune cells in risk assessment of NPs as a reliable strategy for
encouraging non-animal studies approaches, to determine risks that might affect the
human immune system following different exposure scenarios. Taken all together, the
reported observations help to get a more global picture on how IONs alter the human
immune system especially the fact that inflammation, known to involve several
immune cell types, is frequently reported as an undesired effect of IONs. © 2023
AN - rayyan-553781273
AU - Saafane, A.
AU - Girard, D.
DO - 10.1016/j.tiv.2023.105635
KW - In vitro
Iron oxide nanoparticles
Nanotoxicology
Primary human immune cells
Humans
Inflammation
Ions
Magnetic Iron Oxide Nanoparticles
Nanoparticles
caspase 3
caspase 9
catalase
CD63 antigen
CD69 antigen
CD71 antigen
CXCL1 chemokine
ferritin
ferritin heavy chain
ferroportin
gamma interferon
glutathione
histone H3
interleukin 10
interleukin 12
interleukin 17
interleukin 1beta
interleukin 4
interleukin 5
interleukin 6
interleukin 8
leukocyte elastase
maghemite nanoparticle
natural cytotoxicity triggering receptor 2
natural cytotoxicity triggering receptor 3
natural killer cell receptor NKG2D
RANTES
reduced nicotinamide adenine dinucleotide phosphate oxidase
scavenger receptor A
silver nanoparticle
unclassified drug
ion
magnetic iron oxide nanoparticle
nanoparticle
apoptosis
B lymphocyte
basophil
cell migration
cell proliferation
cell protection
cell survival
cell viability
chemical interaction
chromosome aberration
cytokine production
cytokine release
cytotoxicity
degranulation
dendritic cell
DNA damage
endocytosis
eosinophil
genotoxicity
human
immortalized cell line
immune response
immunomodulation
inflammation
internalization (cell)
M1 macrophage
M2 macrophage
macropinocytosis
mast cell
monocyte
natural killer cell
NETosis
neutrophil
nuclear reprogramming
phagocytosis
protein expression
Review
T lymphocyte
Humanities
Humanism
PY - 2023
ST - Interaction between iron oxide nanoparticles (IONs) and primary human immune
cells: An up-to-date review of the literature
TI - Interaction between iron oxide nanoparticles (IONs) and primary human immune
cells: An up-to-date review of the literature
85163173918&doi=10.1016%2fj.tiv.2023.105635&partnerID=40&md5=595753dbef8bcf450dc20a
13eaeda565
VL - 91
ID - 9219
ER -
TY - JOUR
AB - Background: Little is known of how the toxicity of nanoparticles is affected
by the incorporation in complex matrices. We compared the toxic effects of the
titanium dioxide nanoparticle UV-Titan L181 (NanoTiO(2)), pure or embedded in a
paint matrix. We also compared the effects of the same paint with and without
NanoTiO(2). Methods: Mice received a single intratracheal instillation of 18, 54
and 162 mu g of NanoTiO(2) or 54, 162 and 486 mu g of the sanding dust from paint
with and without NanoTiO(2). DNA damage in broncheoalveolar lavage cells and liver,
lung inflammation and liver histology were evaluated 1, 3 and 28 days after
intratracheal instillation. Printex 90 was included as positive control. Results:
There was no additive effect of adding NanoTiO(2) to paints: Therefore the toxicity
of NanoTiO(2) was reduced by inclusion into a paint matrix. NanoTiO(2) induced
inflammation in mice with severity similar to Printex 90. The inflammatory response
of NanoTiO(2) and Printex 90 correlated with the instilled surface area. None of
the materials, except of Printex 90, induced DNA damage in lung lining fluid cells.
The highest dose of NanoTiO(2) caused DNA damage in hepatic tissue 1 day after
intratracheal instillation. Exposure of mice to the dust from paints with and
without TiO2 was not associated with hepatic histopathological changes. Exposure to
NanoTiO(2) or to Printex 90 caused slight histopathological changes in the liver in
some of the mice at different time points. Conclusions: Pulmonary inflammation and
DNA damage and hepatic histopathology were not changed in mice instilled with
sanding dust from NanoTiO(2) paint compared to paint without NanoTiO(2). However,
pure NanoTiO(2) caused greater inflammation than NanoTiO(2) embedded in the paint
matrix.
AN - rayyan-553781274
AU - Saber, A. T.
AU - Mortensen, A.
AU - Szarek, J.
AU - Jackson, P.
AU - Madsen, A. M.
AU - Jensen, K. A.
AU - Koponen, I. K.
AU - Brunborg, G.
AU - Gutzkow, K. B.
AU - Vogel, U.
AU - Wallin, H.
DO - 10.1186/1743-8977-9-4
KW - Mice
Lung
Dust
PY - 2012
SN - 1743-8977
ST - Nanotitanium dioxide toxicity in mouse lung is reduced in sanding dust from
paint
TI - Nanotitanium dioxide toxicity in mouse lung is reduced in sanding dust from
paint
VL - 9
Y2 - 2 y3 - 2
ID - 9220
ER -
TY - JOUR
AB - BackgroundThe development of nano delivery systems is rapidly emerging area
of nanotechnology applications where nanomaterials (NMs) are employed to deliver
therapeutic agents to specific site in a controlled manner. To accomplish this,
green synthesis of NMs is widely explored as an eco-friendly method for the
development of smart drug delivery system. In the recent times, use of green
synthesized NMs, especially metallic NMs have fascinated the scientific community
as they are excellent carriers for drugs. This work demonstrates optimized green,
biogenic synthesis of gold nanoparticles (AuNPs) for functionalization with
quercetin (QT) and camptothecin (CPT) to enhance potential anti-inflammatory, anti-
cancer and anti-angiogenic activities of these drugs.ResultsGold nanoparticles were
optimally synthesized in 8 min of reaction at 90 degrees C, pH 6, using 4 mM of
HAuCl4 and 4:1 ratio of extract: HAuCl4. Among different capping agents tested,
capping of AuNPs with polyethylene glycol 9000 (PG9) was found best suited prior to
functionalization. PG9 capped AuNPs were optimally functionalized with QT in 1 h
reaction at 70 degrees C, pH 7, using 1200 ppm of QT and 1:4 ratio of AuNPs-PG9:QT
whereas, CPT was best functionalized at RT in 1 h, pH 12, AuNPs-PG9:CPT ratio of
1:1, and 0.5 mM of CPT. QT functionalized AuNPs showed good anti-cancer activity
(IC50 687.44 mu g/mL) against MCF-7 cell line whereas test of anti-inflammatory
activity also showed excellent activity (IC50 287.177 mg/L). The CAM based
assessment of anti-angiogenic activity of CPT functionalized AuNPs demonstrated the
inhibition of blood vessel branching confirming the anti-angiogenic
effect.ConclusionsThus, present study demonstrates that optimally synthesized
biogenic AuNPs are best suited for the functionalization with drugs such as QT and
CPT. The functionalization of these drugs with biogenic AuNPs enhances the
potential anti-inflammatory, anti-cancer and anti-angiogenic activities of these
drugs, therefore can be used in biomedical application.
AN - rayyan-553781275
AU - Sadalage, P. S.
AU - Patil, R. V.
AU - Havaldar, D. V.
AU - Gavade, S. S.
AU - Santos, A. C.
AU - Pawar, K. D.
DO - 10.1186/s12951-021-00836-1
IS - 1
PY - 2021
SN - 1477-3155
ST - Optimally biosynthesized, PEGylated gold nanoparticles functionalized with
quercetin and camptothecin enhance potential anti-inflammatory, anti-cancer and
anti-angiogenic activities
TI - Optimally biosynthesized, PEGylated gold nanoparticles functionalized with
quercetin and camptothecin enhance potential anti-inflammatory, anti-cancer and
anti-angiogenic activities
VL - 19
Y2 - 3 y3 - 25
ID - 9221
ER -
TY - JOUR
AB - BACKGROUND: Silver and zinc oxide (ZnO) nanoparticles have recently become
common to coat ligatures in order to take advantage of positive properties of
nanoparticles, although there are concerns about their cytotoxicity. This study
tended to compare subcutaneous inflammatory response induced by elastomeric
orthodontic ligatures coated with silver and ZnO nanoparticles with a control group
in rats. MATERIALS AND METHODS: In this in vitro and animal cross-sectional
descriptive-analytical study, silver nanoparticles were synthesized by chemical
reduction of silver nitrate solution in the presence of sodium borohydride and ZnO
nanoparticles by the same method and by chemical reduction of zinc sulfate solution
with sodium hydroxide and were coated on elastomeric ligatures. Subcutaneous
inflammation degrees were assessed after 15 and 30 days and were compared in the
groups by Kruskal-Wallis test and ordinal generalized estimation equation with
exchangeable correlation matrix. All tests were performed with a significance level
(P = 0.05). RESULTS: There was a significant difference in terms of degrees of
inflammation in the groups coated with ZnO nanoparticles (P = 0.003) and silver
nanoparticles (P = 0.04) compared to the control group in 15- and 30-day samples.
Zinc nanoparticles caused 3.22 times more inflammation than silver nanoparticles (P
= 0.053). The decrease in inflammation was significant over time in all groups (P =
0.001). CONCLUSION: There was a significant more inflammation in the groups
receiving ZnO and silver nanoparticles compared to the control group in 15- and 30-
day samples. Silver nanoparticles are probably safer than zinc nanoparticles for
tissue and a better material to choose for antibacterial effects.
AN - rayyan-553781921
AU - Sadeghian, S.
AU - Ersi, M.
AU - Kalbasi, N.
AU - Najjarian, M.
J2 - Dent Res J (Isfahan)
KW - Ligation
Rats
Zinc
LA - eng
N1 - Department of Orthodontics, Faculty of Dentistry, Islamic Azad University,
Isfahan, Iran.; Department of Orthodontics, Faculty of Dentistry, Islamic Azad
University, Isfahan, Iran.; Department of Oral and Maxillofacial Pathology, Faculty
of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.;
Oral and Maxillofacial Radiologist, Yazd, Iran.
PY - 2022
SN - 1735-3327 (Print)
SP - 97
ST - Comparison of subcutaneous inflammatory response induced by elastomeric
orthodontic ligatures coated with silver and zinc oxide nanoparticles with control
group on rats
T2 - Dental research journal
TI - Comparison of subcutaneous inflammatory response induced by elastomeric
orthodontic ligatures coated with silver and zinc oxide nanoparticles with control
group on rats
VL - 19
ID - 9842
ER -
TY - JOUR
AB - The use of natural polymers and electrospinning as a new method of wound
dressing production is one of the things that can revolutionize the medical world.
Due to the importance of wound healing and characteristics such as anti-
inflammatory and antimicrobial properties, it is possible to use natural compounds
such as fungi and metabolites derived from them to produce wound dressing. In this
study, schizophyllan (SPG) as an extracellular polysaccharide was extracted from
Iranian Schizophyllum commune (NCBI MG761830) and then the silver nanoparticles
(AgNPs) were produced by the in-situ method in 1.5 % SPG solution. Afterward, they
were combined with polyvinyl alcohol 10 % (PVA) polymer to strengthen the fiber
structure. We investigated the properties of nanofibers containing PVA/SPG-AgNPs
and PVA/SPG20 %. The physicochemical properties of two fibers were investigated by
SEM, TEM, FTIR, contact angle, water uptake, nanoparticle release, and biological
test (antibacterial, and MTT). The diameter of the nanofiber-containing the AgNPs
was about 169 nm and the other nanofiber was about 212 nm. The highest inhibition
of the growth of the bacterium by PVA/SPG-AgNPs against E. coli and S. aureus was
about 88.34 % and 64.7 %, respectively. The silver ion release from PVA/SPG-AgNPs
nanofibers was 21 μg/ml after fifth day. Both nanofibers had no toxic effect on
L929 fibroblast cells. © 2019, The Korean Fiber Society.
AN - rayyan-553781276
AU - Safaee-Ardakani, M. R.
AU - Hatamian-Zarmi, A.
AU - Sadat, S. M.
AU - Mokhtari-Hosseini, Z. B.
AU - Ebrahimi-Hosseinzadeh, B.
AU - Kooshki, H.
AU - Rashidiani, J.
DO - 10.1007/s12221-019-9388-8
IS - 12
KW - Cytotoxicity
In situ
Nanofiber
Schizophyllan
Silver nanoparticle
Cells
Contact Angle
Fibers
Natural Polymers
Polyvinyl Acetate
Release
Silver
Test Methods
Cell culture
Contact angle
Escherichia coli
Functional polymers
Metabolites
Metal ions
Metal nanoparticles
Natural polymers
Anti-inflammatory and antimicrobial properties
Extracellular polysaccharides
In-situ preparations
Natural compounds
Schizophyllum commune
Silver ion release
Nanofibers
Wound Healing
PY - 2019
SP - 2493-2502
ST - In situ Preparation of PVA/Schizophyllan-AgNPs Nanofiber as Potential of
Wound Healing: Characterization and Cytotoxicity
T2 - Fibers and Polymers
TI - In situ Preparation of PVA/Schizophyllan-AgNPs Nanofiber as Potential of
Wound Healing: Characterization and Cytotoxicity
85077066998&doi=10.1007%2fs12221-019-9388-
8&partnerID=40&md5=095e2a861a1027874fefd1db21a66b24
VL - 20
ID - 9222
ER -
TY - JOUR
AB - A cutaneous wound is caused by various injuries in the skin, which can be
wrapped with an efficient dressing. Electrospinning is a straightforward adjustable
technique that quickly and continuously generates nanofibrous wound dressings
containing antibacterial and anti-inflammatory agents to promote wound healing. The
present study investigated the physicochemical and biological properties of
bromelain (BRO)- and silver nanoparticle (Ag NPs)-loaded gel-based electrospun
polycaprolactone/chitosan (PCL/CS) nanofibrous dressings for wound-healing
applications. Electron microscopy results showed that the obtained nanofibers (NFs)
had a uniform and homogeneous morphology without beads with an average diameter of
176 ± 63 nm. The FTIR (Fourier transform infrared) analysis exhibited the loading
of the components. Moreover, adding BRO and Ag NPs increased the tensile strength
of the NFs up to 4.59 MPa. BRO and Ag NPs did not significantly affect the
hydrophilicity and toxicity of the obtained wound dressing; however, the
antibacterial activity against E. coli and S. aureus bacteria was significantly
improved. The in vivo study showed that the wound dressing containing BRO and Ag
NPs improved the wound-healing process within one week compared to other groups.
Therefore, gel-based PCL/CS nanofibrous dressings containing BRO and Ag NPs could
be a promising solution for healing skin wounds.
AN - rayyan-553782210
AU - Saghafi, Y.
AU - Baharifar, H.
AU - Najmoddin, N.
AU - Asefnejad, A.
AU - Maleki, H.
AU - Sajjadi-Jazi, S. M.
AU - Bonkdar, A.
AU - Shams, F.
AU - Khoshnevisan, K.
DO - 10.3390/gels9080672
IS - 8
J2 - Gels
KW - Wound Healing
Bromelains
Bandages
LA - eng
N1 - Department of Biomedical Engineering, Science and Research Branch, Islamic
Azad University, Tehran 1477893855, Iran.; Department of Biomedical Engineering,
Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran.;
Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad
University, Tehran 1477893855, Iran.; Research and Development Team, Evolution
Wound Dressing (EWD) Startup Co., Tehran 1983963113, Iran.; Department of
Biomedical Engineering, Science and Research Branch, Islamic Azad University,
Tehran 1477893855, Iran.; Department of Biomedical Engineering, Science and
Research Branch, Islamic Azad University, Tehran 1477893855, Iran.; Research and
Development Team, Evolution Wound Dressing (EWD) Startup Co., Tehran 1983963113,
Iran.; Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah
University of Medical Sciences, Kermanshah 6715847141, Iran.; Research and
Development Team, Evolution Wound Dressing (EWD) Startup Co., Tehran 1983963113,
Iran.; Cell Therapy and Regenerative Medicine Research Center, Endocrinology and
Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical
Sciences, Tehran 1411713137, Iran.; Medical Nanotechnology and Tissue Engineering
Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1983963113,
Iran.; Department of Tissue Engineering and Applied Cell Sciences, School of
Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences,
Tehran 1983963113, Iran.; Department of Tissue Engineering and Applied Cell
Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University
of Medical Sciences, Tehran 1983963113, Iran.; Research and Development Team,
Evolution Wound Dressing (EWD) Startup Co., Tehran 1983963113, Iran.; Medical
Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University
of Medical Sciences, Tehran 1983963113, Iran.; Department of Tissue Engineering and
Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti
University of Medical Sciences, Tehran 1983963113, Iran.
PY - 2023
ST - Bromelain- and Silver Nanoparticle-Loaded Polycaprolactone/Chitosan
Nanofibrous Dressings for Skin Wound Healing
T2 - Gels (Basel, Switzerland)
TI - Bromelain- and Silver Nanoparticle-Loaded Polycaprolactone/Chitosan
Nanofibrous Dressings for Skin Wound Healing
VL - 9
Y2 - 8 y3 - 19
ID - 10120
ER -
TY - JOUR
AB - Nanoparticles have numerous biomedical applications including, but not
limited to, targeted drug delivery, diagnostic imaging, sensors, and implants for a
wide range of diseases including cancer, diabetes, heart disease, and tuberculosis.
Although the mode of delivery of the nanoparticles depends on the application and
the disease, the nanoparticles are often in immediate contact with the systemic
circulation either because of intravenous administration or their ability to enter
the bloodstream with relative ease or their longer survival time in circulation.
Once in circulation, the nanoparticles may elicit unintended hemostatic and
inflammatory responses, and hence the design of nanoparticles for therapeutic
applications should take broad hemocompatibility concerns into consideration. In
this review, we present the principles underlying the structural and functional
design of various classes of nanoparticles that are currently approved by the US
Food and Drug Administration, categorize these particles based on their
interactions with cardiovascular tissues and ensuing adverse events, and also
describe various in vitro assays that may be used evaluate their hemocompatibility.
AN - rayyan-553781277
AU - Saha, A. K.
AU - Zhen, M. Y. S.
AU - Erogbogbo, F.
AU - Ramasubramanian, A. K.
DO - 10.1055/s-0039-1688491
IS - 5
PY - 2020
SN - 0094-6176 1098-9064
SP - 637-651
ST - Design Considerations and Assays for Hemocompatibility of FDA-Approved
Nanoparticles
T2 - SEMINARS IN THROMBOSIS AND HEMOSTASIS
TI - Design Considerations and Assays for Hemocompatibility of FDA-Approved
Nanoparticles
VL - 46
Y2 - 7
ID - 9223
ER -
TY - JOUR
AB - Despite much recent progress, prostate cancer continues to represent a major
cause of cancer-related mortality and morbidity in men. Prostate cancer is the most
common nonskin neoplasm and second leading cause of death in men. 6-Shogaol (6-
SHO), a potent bioactive compound in ginger (Zingiber officinale Roscoe), has been
shown to possess anti-inflammatory and anticancer activity. In the present study,
the effect of 6-SHO on the growth of prostate cancer cells was investigated. 6-SHO
effectively reduced survival and induced apoptosis of cultured human (LNCaP, DU145,
and PC3) and mouse (HMVP2) prostate cancer cells. Mechanistic studies revealed that
6-SHO reduced constitutive and interleukin (IL)-6-induced STAT3 activation and
inhibited both constitutive and TNF-α-induced NF-κB activity in these cells. In
addition, 6-SHO decreased the level of several STAT3 and NF-κB-regulated target
genes at the protein level, including cyclin D1, survivin, and cMyc and modulated
mRNA levels of chemokine, cytokine, cell cycle, and apoptosis regulatory genes (IL-
7, CCL5, BAX, BCL2, p21, and p27). 6-SHO was more effective than two other
compounds found in ginger, 6-gingerol, and 6-paradol at reducing survival of
prostate cancer cells and reducing STAT3 and NF-κB signaling. 6-SHO also showed
significant tumor growth inhibitory activity in an allograft model using HMVP2
cells. Overall, the current results suggest that 6-SHO may have potential as a
chemopreventive and/or therapeutic agent for prostate cancer and that further study
of this compound is warranted.
AN - rayyan-553782352
AU - Saha, A.
AU - Blando, J.
AU - Silver, E.
AU - Beltran, L.
AU - Sessler, J.
AU - DiGiovanni, J.
DO - 10.1158/1940-6207.CAPR-13-0420
IS - 6
J2 - Cancer Prev Res (Phila)
KW - Animals
Catechols/*therapeutic use
Cell Line, Tumor
Cell Proliferation/*drug effects
Down-Regulation/drug effects
Food, Preserved
*Ginger/chemistry
Humans
Male
Mice
NF-kappa B/*antagonists & inhibitors/metabolism
Plant Extracts/*therapeutic use
Prostatic Neoplasms/*drug therapy/metabolism/*pathology
STAT3 Transcription Factor/*antagonists & inhibitors/metabolism
Signal Transduction/drug effects
Prostatic Neoplasms
Prostate
LA - eng
N1 - Authors' Affiliations: Division of Pharmacology and Toxicology and Department
of Nutritional Sciences, Dell Pediatric Research Institute; and Department of
Chemistry, The University of Texas at Austin, Austin, Texas.; Authors'
Affiliations: Division of Pharmacology and Toxicology and Department of Nutritional
Sciences, Dell Pediatric Research Institute; and Department of Chemistry, The
University of Texas at Austin, Austin, Texas.; Authors' Affiliations: Division of
Pharmacology and Toxicology and Department of Nutritional Sciences, Dell Pediatric
Research Institute; and Department of Chemistry, The University of Texas at Austin,
Austin, Texas.; Authors' Affiliations: Division of Pharmacology and Toxicology and
Department of Nutritional Sciences, Dell Pediatric Research Institute; and
Department of Chemistry, The University of Texas at Austin, Austin, Texas.;
Authors' Affiliations: Division of Pharmacology and Toxicology and Department of
Nutritional Sciences, Dell Pediatric Research Institute; and Department of
Chemistry, The University of Texas at Austin, Austin, Texas.; Authors'
Affiliations: Division of Pharmacology and Toxicology and Department of Nutritional
Sciences, Dell Pediatric Research Institute; and Department of Chemistry, The
University of Texas at Austin, Austin, TexasAuthors' Affiliations: Division of
Pharmacology and Toxicology and Department of Nutritional Sciences, Dell Pediatric
Research Institute; and Department of Chemistry, The University of Texas at Austin,
Austin, Texas john.digiovanni@austin.utexas.edu.
PY - 2014
SP - 627-38
ST - 6-Shogaol from dried ginger inhibits growth of prostate cancer cells both in
vitro and in vivo through inhibition of STAT3 and NF-κB signaling
T2 - Cancer prevention research (Philadelphia, Pa.)
TI - 6-Shogaol from dried ginger inhibits growth of prostate cancer cells both in
vitro and in vivo through inhibition of STAT3 and NF-κB signaling
VL - 7
Y2 - 6
ID - 10260
ER -
TY - JOUR
AB - Excessive reactive oxygen species (ROS) and unresolved inflammations are the
major causes of impaired wound healing as they overwhelm the cellular antioxidant
system and impede the healing process. In this study, we examined the application
of Prussian blue (PB) nanozyme as a novel material for cutaneous wound healing
through the alleviation of excessive ROS and inflammation modulation. The PB
nanoparticles not only exhibited hydrogen peroxide (H2O2) degradation activity but
also showed strong superoxide scavenging ability. PB nanozyme mitigated the
intracellular ROS at a high oxidative stress environment, resulting in a pronounced
cytoprotective effect. Moreover, PB nanozyme also displayed significant anti-
inflammatory activity, as evident from the suppression of inflammatory mediators in
the lipopolysaccharide (LPS) induced macrophage cells. Encouraged by the in vitro
results, we evaluated the in vivo therapeutic efficacy of PB nanozyme in a full
thickness cutaneous wound model combined with LPS treatment to mimic bacterial
infection. The beneficial effects of topically applied PB nanozyme on wound healing
and tissue regeneration were evident compared to the control. The periodical
administration of a low amount (50 mu g x 4) of PB nanoparticles exhibited faster
wound closure as well as collagen deposition, maturation, and organization.
Moreover, the PB treatment effectively induced the differentiation of
keratinocytes, enhanced the neovascularization, and reduced macrophage burden in
the entire wound site. Thus, PB nanozyme not only accelerated the healing process
in an infection-mimicking cutaneous wound model but also exhibited tissue
regeneration characteristics owing to the synergistic effect of ROS-scavenging and
anti-inflammatory activities.
AN - rayyan-553781279
AU - Sahu, A.
AU - Jeon, J.
AU - Lee, M. S.
AU - Yang, H. S.
AU - Tae, G.
DO - 10.1016/j.msec.2020.111596
KW - Wound Healing
Antioxidants
Skin
PY - 2021
SN - 0928-4931 1873-0191
ST - Antioxidant and anti-inflammatory activities of Prussian blue nanozyme
promotes full-thickness skin wound healing
T2 - MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
TI - Antioxidant and anti-inflammatory activities of Prussian blue nanozyme
promotes full-thickness skin wound healing
VL - 119
Y2 - 2
ID - 9224
ER -
TY - JOUR
AB - Inulin is a versatile, water-soluble polysaccharide that is commonly
available in nature. In the pharmaceutical industry, the non-digestible function of
inulin has made it attractive. Inulin is granted with GRAS status by the FDA and
more than 30,000 plants in nature store inulin as a carbohydrate. The chicory is
the key plant source of inulin out of all sources. It can be used as the sugar or
fat replacer in the processed foods to influence the desirable characteristics.
Good biocompatibility, essential chemical properties, and a wide variety of
bioactivities have rendered inulin an outstanding natural nutrient. Regulating
blood sugar, antioxidant, anticancer is some of the biological activities of
inulin. Inulin can also be a carrier for colon/tumor targeting, as only specific
enzymes in the colon zhydrolyze the inulin. It allows the growth of micro-flora,
the good bacteria in the gut. Inulin is considered as a prebiotic as it is
fermented by bacteria that normalize the colon. This review offers an in-depth
insight into its novel Pharmaceutical applications as well as sources, processing,
physicochemical properties, and nutritional and physiological activities. The
chemically modified inulin is gaining a specific interest in the pharmaceutical
field with its outstanding properties which are discussed in this review. © 2021
The Authors. Pnces Pvt Ltd.
AN - rayyan-553781280
AU - Sai Kishan, C.
AU - Akhila, A. R.
AU - Sahoo, S.
AU - Kulkarni, P. K.
DO - 10.22159/ijap.2021v13i3.40863
IS - 3
KW - Inulin
Pharmaceutical applications
Prebiotics
acrylic acid
alcohol
antioxidant
aspartame
calcium
calcium ion
carbohydrate
carbon dioxide
catechin
cholesterol
dextran
dextran sulfate
doxorubicin
epirubicin
fenofibrate
free radical
fructose oligosaccharide
galactose oligosaccharide
gastrointestinal hormone
glucose
glucose derivative
gold nanoparticle
hemagglutinin
hemoglobin A1c
hot water
hydrogel
indometacin
influenza vaccine
intestine enzyme
inulin
inulinase
irbesartan
irinotecan
lactic acid
lactulose
lipoprotein
magnesium
mannitol
methotrexate
microsphere
nanoparticle
oligomer
ornidazole
ornithine decarboxylase
paclitaxel
poloxamer
polymer
polysaccharide
polysorbate
prebiotic agent
short chain fatty acid
silver nanoparticle
sorbitol
stabilizing agent
sucrose
sweetening agent
tanshinone
tetrahydrocannabinol
trehalose
triacylglycerol
aberrant crypt focus
acquired immune deficiency syndrome
anorexia
atherosclerosis
bacterium
biocompatibility
biological activity
body weight loss
Caco-2 cell line
calcium blood level
chicory
cholesterol blood level
colon
colon cancer
colon cell line
colorectal cancer
constipation
Crohn disease
crystal
diabetes mellitus
dietary fiber
drug industry
drug release
drug solubility
drug stability
drug therapy
energy metabolism
enteropathy
estimated glomerular filtration rate
evaporation
extraction
fatty liver
fermentation
freeze drying
freezing point
glucagon release
glucose blood level
heart disease
hydrolysis
hypercholesterolemia
hyperlipidemia
hypertension
hypertriglyceridemia
hypoglycemia
hypothalamus
immune system
Influenza virus
intestine
intestine flora
intestine lymphatic tissue
intestine mucosa
Lactobacillus
large intestine
lipogenesis
magnesium blood level
metabolic activity assay
micelle
microbial growth
microfiltration
microflora
natural killer cell
neuroblastoma
nonhuman
nutritional value
obesity
pancreas cancer
phagocytosis
physical chemistry
polymerization
pulsed electric field
purification
rectum cancer
Review
shelf life
skin cancer
small intestine
systematic review
T lymphocyte
thermostability
triacylglycerol blood level
ulcerative colitis
ultrasound
upper gastrointestinal tract
vaccination
viscosity
wettability
PY - 2021
SP - 30-38
ST - A comprehensive review on pharmaceutical and nutritional applications of
inulin
T2 - International Journal of Applied Pharmaceutics
TI - A comprehensive review on pharmaceutical and nutritional applications of
inulin
85107015288&doi=10.22159%2fijap.2021v13i3.40863&partnerID=40&md5=8643bd6e01f1844017
c3282b987bdeaa
VL - 13
ID - 9225
ER -
TY - COMP
AB - Adherent bacterial biofilms have been implicated in the irreversible
contamination of implanted medical devices. We evaluated the resistance of various
tympanostomy (pressure equalization [PE]) tube materials to biofilm formation using
an in vivo model. PE tubes of silicone, silver oxide-impregnated silicone,
fluoroplastic, silver oxide-impregnated fluoroplastic, and ion-bombarded silicone
were inserted into the tympanic membranes of 18 Hartley guinea pigs. Staphylococcus
aureus was then inoculated into the middle ears. An additional 8 guinea pigs were
used as controls; the PE tubes were inserted without middle ear inoculation. All PE
tubes were removed on day 10 and analyzed for bacterial contamination using
culture, immunofluorescence, and scanning electron microscopy (SEM). All infected
ears developed otitis media with otorrhea, but none of the animal control ears
drained. Fluorescence imaging of the animal control tubes showed large cellular
components consistent with inflammation. The infected tubes showed heavy DNA
fluorescence consistent with bacteria and inflammatory cells. All animal control
tubes except the ion-bombarded silicone tubes showed adherent inflammatory film on
SEM. Also, all tubes placed in infected ears except the ion-bombarded silicone
tubes showed adherent bacterial and inflammatory films on SEM. Nonadherent surface
properties such as the ion-bombarded silicone may be helpful in preventing chronic
PE tube contamination.
AN - rayyan-553782214
AU - Saidi, I. S.
AU - Biedlingmaier, J. F.
AU - Whelan, P.
CY - England
DO - 10.1053/hn.1999.v120.a94162
ET - 5
J2 - Otolaryngol Head Neck Surg
KW - Animals
Anti-Bacterial Agents/*chemistry
Biofilms/*growth & development
Catheters, Indwelling/*microbiology
Coated Materials, Biocompatible/*chemistry
Equipment Contamination/*prevention & control
Fluorescent Antibody Technique
Guinea Pigs
Middle Ear Ventilation/*adverse effects/*instrumentation
Otitis Media with Effusion/*microbiology/pathology/surgery
Oxides/*chemistry
Random Allocation
Recurrence
Silicones/*chemistry
Silver Compounds/*chemistry
Staphylococcus aureus/*physiology
Surface Properties
Middle Ear Ventilation
LA - eng
N1 - Division of Otolaryngology-Head and Neck Surgery, Department of Surgery,
University of Maryland Medical System, Baltimore, USA.
PY - 1999
SN - 0194-5998 (Print)
SP - 621-7
ST - In vivo resistance to bacterial biofilm formation on tympanostomy tubes as a
function of tube material
T2 - Otolaryngology--head and neck surgery : official journal of American Academy
of Otolaryngology-Head and Neck Surgery
TI - In vivo resistance to bacterial biofilm formation on tympanostomy tubes as a
function of tube material
VL - 120
Y2 - 5
ID - 10124
ER -
TY - JOUR
AB - The impact of household processes on fenugreek leaves and seeds has been
analyzed for total phenolic (TP) and total flavonoid content (TF), and in-vitro
biological activities such as antioxidant, antimicrobial, and anti-inflammatory
properties. Processes included air-drying for leaves and germinating, soaking, and
boiling for seeds. Air-dried fenugreek leaves (ADFL) had high TP (15.27 mg GAE g(-
1) D.W.) and TF (7.71 mg QE g(-1) D.W.) (milligram quercetin equivalents per gram
dry weight). The TP contents of unprocessed, germinated, soaked, and boiled seeds
were 6.54, 5.60, 4.59, and 3.84 mg gallic acid equivalents per gram of dry weight
(mg GAE g(-1) D.W.), respectively. The TF contents in unprocessed fenugreek seeds,
germinated fenugreek seeds, soaked fenugreek seeds, and boiled fenugreek seeds
(BFS) were 4.23, 2.11, 2.10, and 2.33 mg QE g(-1) D.W., respectively. Sixteen
phenolic and nineteen flavonoid compounds has been identified using high-
performance liquid chromatography. Antioxidant activity using 2,2-diphenyl-1-
picrylhydrazil (DPPH center dot), 2,2-azinobis (3-ethylbenothiazoline-6-sulfonic
acid (ABTS(+center dot)), and ferric reducing antioxidant power (FRAP(center dot))
assays indicated that ADFL had the highest activity. Antimicrobial activity has
been evaluated against each of the eight pathogenic bacterial and fungal strains.
ADFL showed the strongest activity with minimum inhibitory concentrations values
ranging from 0.03 to 1.06 and 0.04 to 1.18 mg ml(center dot 1) against bacterial
and fungal strains, respectively. Anti-inflammatory activity was evaluated in-vitro
against RAW 264.7 macrophage cells using the nitric oxide (NO) assay. Results
revealed that ADFL had the highest cytotoxicity and anti-inflammatory activity
according to the NO assay. Household processes significantly reduced the in-vitro
biological properties of processed seeds.
AN - rayyan-553781283
AU - Salam, S. G. A.
AU - Rashed, M. M.
AU - Ibrahim, N. A.
AU - Rahim, E. A. A.
AU - Aly, T. A. A.
AU - Al-Farga, A.
DO - 10.1038/s41598-023-31888-y
IS - 1
KW - Mass Screening
PY - 2023
SN - 2045-2322
ST - Phytochemical screening and in-vitro biological properties of unprocessed and
household processed fenugreek (Trigonella foenum-graecum Linn.) seeds and leaves
TI - Phytochemical screening and in-vitro biological properties of unprocessed and
household processed fenugreek (Trigonella foenum-graecum Linn.) seeds and leaves
VL - 13
Y2 - 4 y3 - 29
ID - 9228
ER -
TY - JOUR
AB - Prodigiosin is a red pigment produced by Serratia marcescens strain.
Bacterial prodigiosin and its synthetic derivatives are efficacious antioxidants
and proapoptotic agents. This study illustrates a new approach for use of
prodigiosin conjugated silver nanoparticles (PG-AgNP2) against cadmium chloride
(CdCl2) induced neurotoxicity in rats. Rats were (ip) injected with Cd (6.5 mg/kg)
for 7 days with or without PG-AgNP2 (3 mg/kg). The concentration of Cd, DA, NE, 5-
HT, amino acids, NO, MDA, SOD, GSH, catalase, TNF-α, IL-6, Bax, Bcl2 and Caspase-3.
The Cd-intoxicated group showed a significant increase in Cd concentration in brain
tissue, in addition, to an increase in MDA and NO and a decrease in the content of
neurotransmitters (DA, NE, and 5-HT), inhibitory amino acids, and level of all
studied antioxidant enzymes. PG-AgNP2 treatment, significantly reduced Cd-induced
brain tissue injury as indicated by increased antioxidant molecules,
neurotransmitters (DA, NE, and 5-HT), and inhibitory amino acids accompanied by
lower oxidative stress indices (MDA and NO) and excitatory amino acids in brain
tissue. PG-AgNP2 decreased inflammatory mediators including pro-inflammatory
cytokines and prevented the development of apoptosis in the brain tissue. Our
findings suggest that PG-AgNP2 can act as a therapeutic agent against neuronal
impairments associated with Cd exposure. © 2022, Sociedade Brasileira de Ciencia e
Tecnologia de Alimentos, SBCTA. All rights reserved.
AN - rayyan-553781287
AU - Salem, F. E.
AU - Yehia, H. M.
AU - Korany, S. M.
AU - Alarjani, K. M.
AU - Al-Masoud, A. H.
AU - Elkhadragy, M. F.
DO - 10.1590/fst.97322
KW - brain
cd toxicity
nanoparticles
neurotransmitters
prodigiosin
Rats
PY - 2022
ST - Neurotherapeutic effects of prodigiosin conjugated with silver-nanoparticles
in rats exposed to cadmium chloride-induced neurotoxicity
T2 - Food Science and Technology (Brazil)
TI - Neurotherapeutic effects of prodigiosin conjugated with silver-nanoparticles
in rats exposed to cadmium chloride-induced neurotoxicity
85139978986&doi=10.1590%2ffst.97322&partnerID=40&md5=260e3579bf57b30b39e9fdc1e23b3a
b6
VL - 42
ID - 9232
ER -
TY - JOUR
AB - Silver and silver oxides are gaining interest in medical applications for
their prominent antibacterial and antimicrobial potentials. Recent studies suggest
that nanosilver oxide has remarkable anti-inflammatory effects and enhances wound
healing. Nevertheless, its effect on gastric ulcer has not yet been illustrated.
Thus the current study aimed to explore the prospect protective effect of
nanosilver oxide against indomethacin-induced gastric ulcer. A new approach has
been followed to synthesize nanosilver oxide. X-ray diffraction, UV–Vis
spectroscopy and transition electron microscope techniques have been successfully
used to characterize the synthesized nanoparticles. Treatment of ulcerated rats
with different doses of nanosilver oxide especially (175 and 350 ppm/p.o.)
alleviated adverse effects of indomethacin-induced gastric injury as demonstrated
by decreasing ulcer index and elevating % of ulcer inhibition. These positive
effects excelled those exerted by the reference antiulcer drug omeprazole.
Nanosilver oxide suppressed gastric inflammation by reducing myeloperoxidase, tumor
necrosis alpha, interleukin 1beta and interferon gamma. Moreover, nanosilver oxide
halted gastric oxidative stress via inhibiting lipid peroxidation and enhancing
glutathione and paraoxonase-1. Regarding gastric apoptosis, nanosilver oxide down
regulated the expression of caspase 9, tumor protein 53, and nuclear factor kappa B
and allograft inflammatory factor-1 genes. These findings emphasize the
antiulcerogenic potential of nanosilver oxide against indomethacin-induced gastric
ulcers which are multi-factorial including anti-inflammatory, antioxidant and
antiapoptotic effects. © 2017, Springer International Publishing AG, part of
Springer Nature.
AN - rayyan-553781288
AU - Salem, N. A.
AU - Wahba, M. A.
AU - Eisa, W. H.
AU - El-Shamarka, M.
AU - Khalil, W.
DO - 10.1007/s10787-017-0424-2
IS - 4
KW - Apoptosis
Gastric ulcer
Indomethacin
Inflammation
Oxidative stress
Silver oxide nanoparticles
Animals
Anti-Ulcer Agents
Antioxidants
Lipid Peroxidation
Male
Metal Nanoparticles
Oxidative Stress
Oxides
Rats
Rats, Wistar
Silver Compounds
Stomach Ulcer
X-Ray Diffraction
antiulcer agent
aryldialkylphosphatase 1
caspase 9
complementary DNA
gamma interferon
glutathione
indometacin
interleukin 1beta
lead
metal nanoparticle
myeloperoxidase
omeprazole
pepzol
protein p53
RNA
silver oxide nanoparticle
unclassified drug
antioxidant
disilver oxide
oxide
silver derivative
animal experiment
animal model
animal tissue
apoptosis
Article
cell separation
controlled study
down regulation
drug mechanism
gene expression
indomethacin-induced gastric ulcer
leukocyte
lipid peroxidation
male
neutrophil
nonhuman
oxidative stress
priority journal
rat
single drug dose
ulcer index
X ray diffraction
animal
chemically induced
dose response
drug effect
stomach ulcer
Wistar rat
PY - 2018
SP - 1025-1035
ST - Silver oxide nanoparticles alleviate indomethacin-induced gastric injury: a
novel antiulcer agent
T2 - Inflammopharmacology
TI - Silver oxide nanoparticles alleviate indomethacin-induced gastric injury: a
novel antiulcer agent
85036575874&doi=10.1007%2fs10787-017-0424-
2&partnerID=40&md5=e953274bc02b5021580581609f05fc0f
VL - 26
ID - 9233
ER -
TY - JOUR
AB - Cynara humilis is traditionally used to treat skin burns and microbial
infections. However, experimental studies on this plant are rare. Furthermore, the
aim of this study was to investigate the effects of Cynara humilis, a Moroccan
herbal remedy, on the healing of deep second-degree burns in rats with a silver
sulfadiazine group. This research was also carried out to confirm if C. humilis had
antibacterial capabilities. Under typical burn procedures, each rat received a deep
second-degree burn on the upper back. The burns were treated regularly with control
groups (control and control VH), silver sulfadiazine (SDD) in group 3, C. humilis
ethanolic extract (CHEE) in group 4, and C. humilis aqueous extract (CHAE) in group
5. Throughout the treatment, digital photography was used to measure rat responses
to the treatment until day 18. After the scar biopsy at the end of the study,
histological parameters (inflammatory cells, collagen, epithelialization, fibrosis,
and granulation tissue) were assessed. Using the well technique, the antibacterial
activity of the extracts was tested against Staphylococcus aureus CIP 483, Bacillus
subtilis CIP 5262, Escherichia coli CIP 53126, Pseudomonas aeruginosa CIP 82118,
and Salmonella enterica CIP 8039, and the results showed important activities of
the ethanolic and aqueous extracts against the five species tested with MICs of 2
and 4 mg/mL, respectively. In the aqueous extract group, the wound healed faster.
In addition, the healing rate in the C. humilis extracts (CHEA and CHEE) group was
faster than in the silver sulfadiazine and control groups. In the C. humilis group,
maximum wound surface recovery was observed at the same time, as it was not noted
in the silver sulfadiazine group. Pathologically, epithelialization was more marked
in wounds treated with C. humilis extracts (CHE). Angiogenesis and inflammatory
cells were considerably lower in the CHE group than in the silver and other control
groups. However, elastic fibers were considerable in the CHE-treated group. In
histological examination, the C. humilis group had a low incidence of angiogenesis
and inflammation, indicating that this group had less wound scarring. Collagen and
burn wound healing were both faster in the C. humilis group. The findings of this
study suggest that C. humilis, as indicated by traditional medicine, is a promising
natural source for the management of wound healing. © 2023 Najoua Salhi et al.
AN - rayyan-553781289
AU - Salhi, N.
AU - El Guourrami, O.
AU - Rouas, L.
AU - Moussaid, S.
AU - Moutawalli, A.
AU - Benkhouili, F. Z.
AU - Ameggouz, M.
AU - Alshahrani, M. M.
AU - Al Awadh, A. A.
AU - Bouyahya, A.
AU - Faouzi, M. E. A.
AU - Cherrah, Y.
DO - 10.1155/2023/5855948
KW - chloramphenicol
cynara humilis extract
ketamine
plant extract
resazurin
sulfadiazine silver
unclassified drug
angiogenesis
animal cell
animal experiment
animal model
Article
Bacillus subtilis
biopsy
burn
controlled study
Cynara
drug efficacy
epithelization
Escherichia coli
female
herbal medicine
image processing
male
nonhuman
rat
Salmonella enterica
skin toxicity
tissue section
wound healing
Wound Healing
Burns
PY - 2023
ST - Evaluation of the Wound Healing Potential of Cynara humilis Extracts in the
Treatment of Skin Burns
T2 - Evidence-based Complementary and Alternative Medicine
TI - Evaluation of the Wound Healing Potential of Cynara humilis Extracts in the
Treatment of Skin Burns
85158069477&doi=10.1155%2f2023%2f5855948&partnerID=40&md5=5d3b86db5747d89dc72b1a398
d34d478
VL - 2023
ID - 9234
ER -
TY - JOUR
AB - Various assisted reproductive technologies (ART) are applied in the
infertility treatment. However, considerable attention is addressed to use
alternative approaches, such as non-biotechnology, probiotics, and traditional
medicinal plants to treat cancerous and non-cancerous cases of infertility.
Nanotechnology was remarkably aided in treatment, diagnosis, and drug delivery. The
existing data of this technology might demonstrate the enormous potential of
nanomaterials and their viability in clinical trials for the study of reproductive
issues. In order to understand the function of the microbiome in infertility and
the many good effects of probiotics in illnesses such as colon cancer, obesity,
diabetes, and inflammatory bowel disease, research on infertility must be
conducted. Healthy reproductive systems are important for successful fertility in
males and females, and using probiotics can help reduce the associated
complications. Besides, in vivo models are required to determine the probiotics
proper administration, identify the functional species, effective doses,
administration forms, and the effects of their combination with conventional
antibiotics. In addition, medicinal herbs should be explored, notably in the
treatment of male infertility and the improvement of sperm abnormalities. The
antioxidant capacity, anti-inflammatory reactions, increased sperm production, and
increased testosterone levels in the blood are all examples of medicinal plant
benefits. More study is needed to establish specific findings on which substances
are involved and have effective and safe fertility potential. This review presents
an overview of potential applications for nanotechnology, probiotics, and medicinal
plants in infertility, discussing the advantages, their feasibility, and associated
concerns, which demand more investigations to set of clinical applications. © 2023
Bentham Science Publishers.
AN - rayyan-553781291
AU - Salmany, N.
AU - Lotfi, H.
AU - Keyhanmanesh, R.
AU - Ghiasi, R.
DO - 10.2174/1573404818666220427083700
IS - 4
KW - bowel disease
cancer photothermal therapy
Infertility
medicinal plants
nanotechnology
probiotics
antioxidant
cabazitaxel
carboplatin
cisplatin
curcumin
dendrimer
dexamethasone
efavirenz
follitropin
gold nanoparticle
iron oxide
liposome
luteinizing hormone
macrogol
Muellerian inhibiting factor
nanocarrier
nanocomposite
paclitaxel
polymer
probiotic agent
silver nanoparticle
Alzheimer disease
antiviral activity
apoptosis
atherosclerosis
biodegradability
blood brain barrier
Candida albicans
cell death
cell proliferation
chemotherapy
Chlamydia trachomatis
colon cancer
Crocus sativus
diabetes mellitus
drug release
ectopic pregnancy
encapsulation
endometriosis
Escherichia coli
feasibility study
fertility
ginger
Helicobacter pylori
Hepatitis B virus
herbal medicine
human
Human immunodeficiency virus
hysterectomy
infertility
medicinal plant
mouse
Mumps virus
Mycoplasma genitalium
Neisseria gonorrhoeae
nephrotoxicity
neurotoxicity
nonhuman
obesity
osteoporosis
ototoxicity
oxidative stress
photothermal therapy
pomegranate
prostate cancer
reproductive health
Review
semen analysis
sexual transmission
sperm count
testis cancer
trophoblast
uterine cervix cancer
uterus myoma
vagina flora
vaginitis
velvet bean
Wart virus
Plants, Medicinal
Probiotics
PY - 2023
SP - 91-106
ST - An Overview of Potential Applications for Nanotechnology, Probiotics, and
Medicinal Plants in Infertility Problems
T2 - Current Women's Health Reviews
TI - An Overview of Potential Applications for Nanotechnology, Probiotics, and
Medicinal Plants in Infertility Problems
85149803664&doi=10.2174%2f1573404818666220427083700&partnerID=40&md5=cfbaaccdb6e475
b7026977d9e0757348
VL - 19
ID - 9236
ER -
TY - JOUR
AB - Objective(s): The main objective of the current assay was to evaluate the
antibacterial and regenerative effects of hydrogel nanocomposite containing pure
natural zeolite (clinoptilolite) integrated with alginate (Alg) as wound
healing/dressing biomaterials.Materials and Methods: The zeolites were size
excluded, characterized by SEM, DLS, XRD, FTIR, and XRF, and then integrated into
Alg hydrogel followed by calcium chloride crosslinking. The Alg and alginate
zeolite (Alg/Zeo) hydrogel was characterized by swelling and weight loss tests,
also the antibacterial, hemocompatibility, and cell viability tests were performed.
In animal studies, the burn wound was induced on the back of rats and treated with
the following groups: control, Alg hydrogel, and Alg/Zeo hydrogel.Results: The
results showed that the hydrodynamic diameter of zeolites was 367 +/- 0.2 nm.
Zeolites did not show any significant antibacterial effect, however, the hydrogel
nanocomposite containing zeolite had proper swelling as well as hemocompatibility
and no cytotoxicity was observed. Following the creation of a third-degree burn
wound on the back of rats, the results indicated that the Alg hydrogel and Alg/Zeo
nanocomposite accelerated the wound healing process compared with the control
group. Re-epithelialization, granulation tissue thickness, collagenization,
inflammatory cell recruitment, and angiogenesis level were not significantly
different between Alg and Alg/Zeo nanocomposite.Conclusion: These findings revealed
that although the incorporation of zeolites did not induce a significant beneficial
effect in comparison with Alg hydrogel, using zeolite capacity in hydrogel for
loading the antibiotics or other effective compounds can be considered a promising
wound dressing.
AN - rayyan-553781292
AU - Samadian, H.
AU - Vahidi, R.
AU - Salehi, M.
AU - Hosseini-Nave, H.
AU - Shahabi, A.
AU - Zanganeh, S.
AU - Lashkari, M.
AU - Kouhbananinejad, S. M.
AU - Kolarijani, N. R.
AU - Amini, S. M.
AU - Asadi-shekari, M.
AU - Parsa, M. J. M.
DO - 10.22038/IJBMS.2023.68897.15016
IS - 6
KW - Burns
Wound Healing
Hydrogel
Hydrogels
Alginates
PY - 2023
SN - 2008-3866 2008-3874
SP - 708-716
ST - Hydrogel nanocomposite based on alginate/zeolite for burn wound healing: In
vitro and in vivo study
T2 - IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
TI - Hydrogel nanocomposite based on alginate/zeolite for burn wound healing: In
vitro and in vivo study
VL - 26
Y2 - 6
ID - 9237
ER -
TY - JOUR
AB - The new type of annulated imidazolium salt 1-naphthyl-2-pyridin-2-yl-2H-
imidazo[1,5-a] pyridin-4-ylium hexafluorophosphate (1.HPF6) and three novel N-
heterocyclic carbene complexes (NHCs) [Ag(1)(2)][PF6] (2), [Au(1)(2)][PF6] (3), and
[Au(1)Cl-3] 4 have been synthesized and characterized by different spectroscopic
techniques. The solid state structure of 2 has been determined by single crystal X-
ray diffraction studies. The complex 3 has been synthesized via trans-metallation,
whereas complex 4 was obtained via a disproportionation process. The cytotoxicities
of the complexes 2, 3 and 4 were tested in vitro against non-small lung carcinoma
(A549), colorectal carcinoma (HCT-116) and breast adenocarcinoma (MCF-7) cell
lines. The measured IC50 values showed that the Au (I) complex 3 is more potent
than the complexes 2 and 4 as well as cisplatin. (C) 2015 Elsevier B.V. All rights
reserved.
AN - rayyan-553781295
AU - Samanta, T.
AU - Munda, R. N.
AU - Roymahapatra, G.
AU - Nandy, A.
AU - Das Saha, K.
AU - Al-Deyab, S. S.
AU - Dinda, J.
DO - 10.1016/j.jorganchem.2015.05.049
PY - 2015
SN - 0022-328X 1872-8561
SP - 183-191
ST - Silver(I), Gold(I) and Gold(III)-N-Heterocyclic carbene complexes of naphthyl
substituted annelated ligand: Synthesis, structure and cytotoxicity
T2 - JOURNAL OF ORGANOMETALLIC CHEMISTRY
TI - Silver(I), Gold(I) and Gold(III)-N-Heterocyclic carbene complexes of naphthyl
substituted annelated ligand: Synthesis, structure and cytotoxicity
VL - 791
Y2 - 8 y3 - 15
ID - 9240
ER -
TY - JOUR
AB - Diabetic wound infections and pressure ulcers pose a significant challenge to
healthcare providers worldwide. The current study provides new and innovative wound
care products that reduce inflammation, clear infection, and improve healing in an
animal model of pressure ulcers in diabetic rats. Ointment, hydrogel, and nanofiber
dressings were synthesized using 5% turmeric, 1% oregano, and 1% chitosan
nanoparticles and tested for antibacterial and cytotoxicity in vitro, and wound
healing effects in vivo. Turmeric ethanolic extract showed high antioxidant
activity compared to Oregano, Chitosan Nanoparticles, and Alginate silver (p-value
' 0.0001). The ointment and hydrogel formulation (5% Turmeric, 1% Oregano, and 1%
chitosan) showed lower cytotoxicity compared to the commercial Alginate silver
dressing. Ointment, hydrogel formulations, and commercial Alginate silver, showed
significant antibacterial activity with 100% efficacy on both Staphylococcus aureus
and Escherichia coli (p-value ' 0.0001), compared to nanofibers which showed 50%
reduction in bacterial growth (p-value ' 0.0001). The new formulations were tested
in a rat model of pressure ulcers. Ointment and nanofibers achieved complete wound
healing by day 15 compared to the hydrogel and commercial Alginate silver dressing,
which showed higher infection, and the wound remained partially open by day 21. In
conclusion, Turmeric, Oregano extracts, and chitosan nanoparticles can be used for
effective wound dressings in both diabetic and non-diabetic wounds. At relatively
low concentrations, this combination provides a promising new wound treatment
formulation that is antibacterial, anti-inflammatory, and antioxidant. © 2020
AN - rayyan-553781297
AU - Sami, D. G.
AU - Abdellatif, A.
AU - Azzazy, H. M. E.
DO - 10.1080/03639045.2020.1811305
IS - 10
KW - diabetic ulcer
nanomaterial
oregano
turmeric
wound dressing
Wound healing
Animals
Bandages
Curcuma
Origanum
Rats
Ulcer
alginic acid
essential oil
hydrogel
nanofiber
plant extract
antiinfective agent
animal experiment
animal model
animal tissue
Article
Asparagus racemosus
bacterial growth
cell structure
cell viability
comparative study
controlled study
cytotoxicity
diabetic foot
drug formulation
Escherichia coli
hydrodynamics
in vitro study
in vivo study
male
nonhuman
ointment
pathogenesis
pilot study
rat
rat model
skin
zeta potential
animal
bandage
experimental diabetes mellitus
ulcer
PY - 2020
SP - 1613-1621
ST - Turmeric/oregano formulations for treatment of diabetic ulcer wounds
T2 - Drug Development and Industrial Pharmacy
TI - Turmeric/oregano formulations for treatment of diabetic ulcer wounds
85089970974&doi=10.1080%2f03639045.2020.1811305&partnerID=40&md5=96b50549ec41e82aa8
2a2ea91c2e0e7c
VL - 46
ID - 9242
ER -
TY - JOUR
AB - Background For years, silver has been used for the treatment of skin
injuries. Objective The effect of the oral administration of silver nanoparticles
on the wound healing process in male rats was studied. Materials and methods In
this experimental study, 30 Wistar male rats were randomly allocated to three
groups - the control and two silver nanoparticles treatment groups (30ppm and 60ppm
AgNPs concentration). In all rats, the full- thickness wound was induced under
general anesthesia. At 12 days post-wounding, microscopic evaluation of wound
healing - for example inflammatory cells, fibroblasts, angiogenesis and collagen
density - was completed. Results The percentage of wound healing between the
control and the treatment groups on the 12th day was significant (p<0.001).
Moreover, the number of inflammatory cells was significantly higher in the control
than in the treatment groups (p<0.001). The difference between the fibroblast
number and collagen density was more in the treatment groups than in the control
(p<0.001). Also, a considerable difference was observed between the number of
inflammatory cells and fibroblasts in the 60ppm compared to the 30ppm
concentration. Conclusion By inducing anti-inflammatory effects and increasing the
proliferation of fibroblasts and the expression of collagen, silver nanoparticles
at a concentration of 30ppm accelerated the wound healing process.
AN - rayyan-553781298
AU - Samiee-Rad, F.
AU - Sofiabadi, M.
AU - Habibian, Z.
AU - Gheibi, N.
DO - 10.33235/wpr.28.1.8-16
IS - 1
Rats
Wound Healing
PY - 2020
SN - 1837-6304 2202-9729
SP - 8-16
ST - Effects of the oral administration of silver nanoparticles on wound healing
in male rats
T2 - WOUND PRACTICE AND RESEARCH
TI - Effects of the oral administration of silver nanoparticles on wound healing
in male rats
VL - 28
Y2 - 3
ID - 9243
ER -
TY - JOUR
AB - Knee osteoarthritis (KOA) is one of the most commonly encountered
degenerative diseases of the joints in people over 45 years of age. Currently,
there are not any effective therapeutics for KOA,and the only end-point strategy is
total knee arthroplasty (TKA); therefore, KOA is associated with economic burdens
and societal costs. The immune inflammatory response is involved in the occurrence
and development of KOA. We previously established a mouse model of KOA using type
II collagen. Hyperplasia of the synovial tissue was present in the model, alongside
a large number of infiltrated inflammatory cells. Silver nanoparticles have
substantial anti-inflammatory effects and have been widely used in tumor therapy
and surgical drug delivery. Therefore, we evaluated the therapeutic effects of
silver nanoparticles in a collagenase II-induced KOA model. The experimental
results showed that silver nanoparticles significantly reduced synovial hyperplasia
and the infiltration of neutrophils in the synovial tissue. Hence, this work
demonstrates the identification of a novel strategy for OA and provides a
theoretical basis for preventing the progress of KOA.
AN - rayyan-553781302
AU - Sang, Y.
AU - Zhang, J. B.
AU - Liu, C.
AU - Liu, K. H.
AU - Yao, H.
AU - Zhao, H. Q.
AU - Xu, W. B.
AU - Xu, Y. C.
AU - Hou, G.
DO - 10.3791/65111
IS - 196
KW - Mice
Osteoarthritis
PY - 2023
SN - 1940-087X
ST - Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
T2 - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
TI - Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
Y2 - 6
ID - 9247
ER -
TY - JOUR
AB - Metal and metal oxide nanoparticles are often used as industrial catalysts or
to improve product's functional properties. Recent advanced nanotechnology have
been expected to be used in various fields, ranging from sensors, environmental
remediation to biomedicine, medical biology and imaging, etc. However, the growing
use of nanoparticles has led to their release into environment and increased levels
of these particles at nearby sites or the surroundings of their manufacturing
factories become obvious. The toxicity of metal and metal oxide nanoparticles on
humans, animals, and certainly to the environment has become a major concern to our
community. However, controversies still remain with respect to the toxic effects
and the mechanisms of these nanoparticles. The scientific community now feels that
an understanding of the toxic effects is necessary to handle these nanoparticles
and their use. A new discipline, named nanotoxicology, has therefore been developed
that basically refers to the study of the interactions of nanoparticles with
biological systems and also measures the toxicity level related to human health.
Nanoparticles usually generate reactive oxygen species to a greater extent than
micro-sized particles resulting in increased pro-inflammatory reactions and
oxidative stress via intracellular signaling pathways. In this review, we mainly
focus on the routes of exposure of some metal and metal oxide nanoparticles and how
these nanoparticles affect us or broadly the cells of our organs. We would also
like to discuss the responsible mechanism(s) of the nanoparticle-induced reactive
oxygen species mediated organ pathophysiology. A brief introduction of the
characterization and application of these nanoparticles has also been included in
the article.
AN - rayyan-553781303
AU - Sarkar, A.
AU - Ghosh, M.
AU - Sil, P. C.
DO - 10.1166/jnn.2014.8752
IS - 1
Metals
PY - 2014
SN - 1533-4880 1533-4899
SP - 730-743
ST - Nanotoxicity: Oxidative Stress Mediated Toxicity of Metal and Metal Oxide
Nanoparticles
T2 - JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
TI - Nanotoxicity: Oxidative Stress Mediated Toxicity of Metal and Metal Oxide
Nanoparticles
VL - 14
Y2 - 1
ID - 9248
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are incorporated into medical devices for their
anti-microbial characteristics. The potential exposure and toxicity of AgNPs is
unknown due to varying physicochemical particle properties and lack of
toxicological data. The aim of this safety assessment is to derive a provisional
tolerable intake (pTI) value for AgNPs released from blood-contacting medical
devices. A literature review of in vivo studies investigating critical health
effects induced from intravenous (i.v.) exposure to AgNPs was evaluated by the
Annapolis Accords principles and Toxicological Data Reliability Assessment Tool
(ToxRTool). The point of departure (POD) was based on an i.v. 28-day repeated AgNP
(20 nm) dose toxicity study reporting an increase in relative spleen weight in rats
with a 5% lower confidence bound of the benchmark dose (BMDL05) of 0.14 mg/kg
bw/day. The POD was extrapolated to humans by a modifying factor of 1,000 to
account for intraspecies variability, interspecies differences and lack of long-
term toxicity data. The pTI for long-term i.v. exposure to 20 nm AgNPs released
from blood-contacting medical devices was 0.14 pg/kg bw/day. This pTI may not be
appropriate for nanoparticles of other physicochemical properties or routes of
administration. The methodology is appropriate for deriving pTls for nanoparticles
in general. Published by Elsevier Inc.
AN - rayyan-553781307
AU - Savery, L. C.
AU - Vinas, R.
AU - Nagy, A. M.
AU - Pradeep, P.
AU - Merrill, S. J.
AU - Hood, A. M.
AU - Malghan, S. G.
AU - Goering, P. L.
AU - Brown, R. P.
DO - 10.1016/j.yrtph.2017.01.007
PY - 2017
SN - 0273-2300 1096-0295
SP - 108-118
ST - Deriving a provisional tolerable intake for intravenous exposure to silver
nanoparticles released from medical devices
T2 - REGULATORY TOXICOLOGY AND PHARMACOLOGY
TI - Deriving a provisional tolerable intake for intravenous exposure to silver
nanoparticles released from medical devices
VL - 85
Y2 - 4
ID - 9252
ER -
TY - JOUR
AB - Although there are many applications of silver nanoparticles (Ag-NPs) in
human activities, there is still little known about their potential environmental
toxicity, particularly to fish. In the present study, the effects of Ag-NPs on
African catfish (Clarias gariepinus) were studied using melanomacrophage centers as
immunohistological biomarkers. Fish were exposed to 25 mg/L, 50 mg/L and 75 mg/L
100-nm Ag-NPs. We studied the effects on the size and number of melanomacrophage
centers in all target tissues. Many histopathological alterations in those tissues
were observed. The histological changes were represented as dislocation of the
epithelium, dilatation of central veins associated with inflammatory leukocytic
infiltration, necrosis, and pyknotic nuclei of hepatocytes. There was shrinkage of
Malpighian corpuscles, dislocation of nuclei of convoluted tubules, cellular
degeneration, and dispersed infiltration of leukocytes in kidney tissue.
Examination of spleen sections after exposure to Ag-NPs showed rupture within the
red pulp and hemorrhage, dislocation of nuclei, accumulation of inflammatory
leukocytes, and congestion in blood vessels. In conclusion, exposure to Ag-NPs
induced alterations in tissues, suggesting a possible increase in oxidative stress
in those tissues.
AN - rayyan-553781981
AU - Sayed, A. H.
AU - Younes, H. A. M.
DO - 10.1016/j.jmau.2016.07.003
IS - 2
J2 - J Microsc Ultrastruct
KW - Biological Markers
LA - eng
N1 - Department of Zoology, Faculty of Science, Assiut University, 71516 Assiut,
Egypt.; Department of Zoology, Faculty of Science, Assiut University, 71516 Assiut,
Egypt.
PY - 2017
SP - 97-104
ST - Melanomacrophage centers in Clarias gariepinus as an immunological biomarker
for toxicity of silver nanoparticles
T2 - Journal of microscopy and ultrastructure
TI - Melanomacrophage centers in Clarias gariepinus as an immunological biomarker
for toxicity of silver nanoparticles
VL - 5
Y2 - 4
ID - 9898
ER -
TY - JOUR
AB - Wound infections are feared complications due to their potential to increase
healthcare costs and cause mortality since multidrug-resistant bacteria reduce
treatment options. This study reports the development of a carbomer hydrogel
containing biogenic silver nanoparticles (bioAgNPs) and its effectiveness in wound
treatment. This hydrogel showed in vitro bactericidal activity after 2 h, according
to the time-kill assay. It also reduced bacterial contamination in rat wounds
without impairing their healing since the hydrogel hydrophilic groups provided
hydration for the injured skin. The high number of inflammatory cells in the first
days of the skin lesion and the greater degree of neovascularization one week after
wound onset showed that the healing process occurred normally. Furthermore, the
hydrogel-containing bioAgNPs did not cause toxic silver accumulation in the organs
and blood of the rats. This study developed a bioAgNP hydrogel for the treatment of
wounds; it has a potent antimicrobial action without interfering with cicatrization
or causing silver bioaccumulation. This formulation is effective against bacteria
that commonly cause wound infections, such as Pseudomonas aeruginosa and
Staphylococcus aureus, and for which new antimicrobials are urgently needed,
according to the World Health Organization's warning.
AN - rayyan-553782291
AU - Scandorieiro, S.
AU - Kimura, A. H.
AU - de Camargo, L. C.
AU - Gonçalves, M. C.
AU - da Silva, J. V. H.
AU - Risso, W. E.
AU - de Andrade, F. G.
AU - Zaia, Ctbv
AU - Lonni, Aasg
AU - Dos Reis Martinez, C. B.
AU - Durán, N.
AU - Nakazato, G.
AU - Kobayashi, R. K. T.
DO - 10.3390/microorganisms11071815
IS - 7
J2 - Microorganisms
KW - Rats
Wound Healing
Rats, Wistar
Hydrogel
Hydrogels
LA - eng
N1 - Laboratory of Basic and Applied Bacteriology, Department of Microbiology,
Center of Biological Sciences, State University of Londrina, Londrina 86057-970,
Brazil.; Laboratory of Innovation and Cosmeceutical Technology, Department of
Pharmaceutical Sciences, Center of Health Sciences, University Hospital of
Londrina, Londrina 86038-350, Brazil.; Laboratory of Basic and Applied
Bacteriology, Department of Microbiology, Center of Biological Sciences, State
University of Londrina, Londrina 86057-970, Brazil.; Laboratory of Basic and
Applied Bacteriology, Department of Microbiology, Center of Biological Sciences,
State University of Londrina, Londrina 86057-970, Brazil.; Laboratory of Basic and
State University of Londrina, Londrina 86057-970, Brazil.; Laboratory of
Histopathological Analysis, Department of Physiological Sciences, Center of
Biological Sciences, State University of Londrina, Londrina 86057-970, Brazil.;
Laboratory of Animal Ecophysiology, Department of Physiological Sciences, Center of
Biological Sciences, State University of Londrina, Londrina 86057-970, Brazil.;
Laboratory of Histopathological Analysis, Department of Physiological Sciences,
Center of Biological Sciences, State University of Londrina, Londrina 86057-970,
Brazil.; Laboratory of Neuroendocrine Physiology and Metabolism, Department of
Physiological Sciences, Center of Biological Sciences, State University of
Londrina, Londrina 86057-970, Brazil.; Laboratory of Innovation and Cosmeceutical
Technology, Department of Pharmaceutical Sciences, Center of Health Sciences,
University Hospital of Londrina, Londrina 86038-350, Brazil.; Laboratory of Animal
Ecophysiology, Department of Physiological Sciences, Center of Biological Sciences,
State University of Londrina, Londrina 86057-970, Brazil.; Institute of Biology,
State University of Campinas, Campinas 13083-862, Brazil.; Laboratory of Basic and
State University of Londrina, Londrina 86057-970, Brazil.; Laboratory of Basic and
State University of Londrina, Londrina 86057-970, Brazil.
PY - 2023
SN - 2076-2607 (Print)
ST - Hydrogel-Containing Biogenic Silver Nanoparticles: Antibacterial Action,
Evaluation of Wound Healing, and Bioaccumulation in Wistar Rats
T2 - Microorganisms
TI - Hydrogel-Containing Biogenic Silver Nanoparticles: Antibacterial Action,
Evaluation of Wound Healing, and Bioaccumulation in Wistar Rats
VL - 11
Y2 - 7 y3 - 15
ID - 10199
ER -
TY - JOUR
AB - BACKGROUND: The objective of this study was to examine the threshold fibre
length for the onset of pulmonary inflammation after aspiration exposure in mice to
four different lengths of silver nanowires (AgNW). We further examined the effect
of fibre length on macrophage locomotion in an in vitro wound healing assay. We
hypothesised that exposure to longer fibres causes both increased inflammation and
restricted mobility leading to impaired clearance of long fibres from the lower
respiratory tract to the mucociliary escalator in vivo. METHODS: Nine week old
female C57BL/6 strain mice were exposed to AgNW and controls via pharyngeal
aspiration. The dose used in this study was equalised to fibre number and based on
50 μg/ mouse for AgNW(14). To examine macrophage migration in vitro a wound healing
assay was used. An artificial wound was created in a confluent layer of bone marrow
derived macrophages by scraping with a pipette tip and the number of cells
migrating into the wound was monitored microscopically. The dose was equalised for
fibre number and based on 2.5 μg/cm(2) for AgNW(14). RESULTS: Aspiration of AgNW
resulted in a length dependent inflammatory response in the lungs with threshold at
a fibre length of 14 μm. Shorter fibres including 3, 5 and 10 μm elicited no
significant inflammation. Macrophage locomotion was also restricted in a length
dependent manner whereby AgNW in the length of ≥5 μm resulted in impaired motility
in the wound closure assay. CONCLUSION: We demonstrated a 14 μm cut-off length for
fibre-induced pulmonary inflammation after aspiration exposure and an in vitro
threshold for inhibition of macrophage locomotion of 5 μm. We previously reported a
threshold length of 5 μm for fibre-induced pleural inflammation. This difference in
pulmonary and pleural fibre- induced inflammation may be explained by differences
in clearance mechanism of deposited fibres from the airspaces compared to the
pleural space. Inhibition of macrophage migration at long fibre lengths could
account for their well-documented long term retention in the lungs compared to
short fibres. Knowledge of the threshold length for acute pulmonary inflammation
contributes to hazard identification of nanofibres.
AN - rayyan-553782233
AU - Schinwald, A.
AU - Chernova, T.
AU - Donaldson, K.
DO - 10.1186/1743-8977-9-47
KW - Animals
Bronchoalveolar Lavage Fluid/cytology
Cell Movement/*drug effects
Cells, Cultured
Female
Macrophages, Alveolar/*drug effects
Mice
Mice, Inbred C57BL
Nanowires/chemistry/*toxicity
Particle Size
Phagocytosis/drug effects
Pneumonia/*chemically induced/pathology
Surface Properties
Macrophages
Inflammation
Pneumonia
LA - eng
N1 - MRC/University of Edinburgh, Centre for Inflammation Research, Queen's
Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
PY - 2012
SP - 47
ST - Use of silver nanowires to determine thresholds for fibre length-dependent
pulmonary inflammation and inhibition of macrophage migration in vitro
TI - Use of silver nanowires to determine thresholds for fibre length-dependent
pulmonary inflammation and inhibition of macrophage migration in vitro
VL - 9
Y2 - 12 y3 - 2
ID - 10143
ER -
TY - JOUR
AB - BACKGROUND: Frustrated phagocytosis has been stated as an important factor in
the initiation of an inflammatory response after fibre exposure. The length of
fibrous structures has been linked to the potential of fibres to induce adverse
health effects for at least 40 years. However, we only recently reported for the
first time the threshold length for fibre-induced inflammation in the pleural space
and we implicated frustrated phagocytosis in the pro-inflammatory effects of long
fibres. This study extends the examination of the threshold value for frustrated
phagocytosis using well-defined length classes of silver nanowires (AgNW) ranging
from 3-28 μm and describes in detail the morphology of frustrated phagocytosis
using a novel technique and also describes compartmentalisation of fibres in the
pleural space. METHODS: A novel technique, backscatter scanning electron microscopy
(BSE) was used to study frustrated phagocytosis since it provides high-contrast
detection of nanowires, allowing clear discrimination between the nanofibres and
other cellular features. A human monocyte-derived macrophage cell line THP-1 was
used to investigate cell-nanowire interaction in vitro and the parietal pleura, the
site of fibre retention after inhalation exposure was chosen to visualise the cell-
fibre interaction in vivo after direct pleural installation of AgNWs. RESULTS: The
length cut-off value for frustrated phagocytosis differs in vitro and in vivo.
While in vitro frustrated phagocytosis could be observed with fibres≥14 μm, in vivo
studies showed incomplete uptake at a fibre length of ≥10 μm. Recently we showed
that inflammation in the pleural space after intrapleural injection of the same
nanofibre panel occurs at a length of ≥5 μm. This onset of inflammation does not
correlate with the onset of frustrated phagocytosis as shown in this study, leading
to the conclusion that intermediate length fibres fully enclosed within macrophages
as well as frustrated phagocytosis are associated with a pro-inflammatory state in
the pleural space. We further showed that fibres compartmentalise in the
mesothelial cells at the parietal pleura as well as in inflammatory cells in the
pleural space. CONCLUSION: BSE is a useful way to clearly distinguish between
fibres that are, or are not, membrane-bounded. Using this method we were able to
show differences in the threshold length at which frustrated phagocytosis occurred
between in vitro and in vivo models. Visualising nanowires in the pleura
demonstrated at least 2 compartments--in leukocyte aggregations and in the
mesothelium--which may have consequences for long term pathology in the pleural
space including mesothelioma.
AN - rayyan-553782070
AU - Schinwald, A.
AU - Donaldson, K.
DO - 10.1186/1743-8977-9-34
KW - Animals
Bronchoalveolar Lavage Fluid/chemistry/cytology
Cells, Cultured
Electron Probe Microanalysis
Epithelium/*drug effects/metabolism/ultrastructure
Female
Humans
Macrophages/*drug effects/physiology/ultrastructure
Mice
Mice, Inbred C57BL
Microscopy, Electron, Scanning/methods
Nanowires/*toxicity
Particle Size
Phagocytosis/*drug effects/physiology
Pleura/chemistry/*drug effects/metabolism/ultrastructure
LA - eng
N1 - Centre for Inflammation Research, Queen's Medical Research Institute,
MRC/University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
PY - 2012
SP - 34
ST - Use of back-scatter electron signals to visualise cell/nanowires interactions
in vitro and in vivo; frustrated phagocytosis of long fibres in macrophages and
compartmentalisation in mesothelial cells in vivo
TI - Use of back-scatter electron signals to visualise cell/nanowires interactions
in vitro and in vivo; frustrated phagocytosis of long fibres in macrophages and
compartmentalisation in mesothelial cells in vivo
VL - 9
Y2 - 8 y3 - 28
ID - 9984
ER -
TY - JOUR
AB - Suspicion has been raised that high aspect ratio nanoparticles or nanofibers
might possess asbestos-like pathogenicity. The pleural space is a specific target
for disease in individuals exposed to asbestos and by implication of nanofibers.
Pleural effects of fibers depends on fiber length, but the key threshold length
beyond which adverse effects occur has never been identified till now because all
asbestos and vitreous fiber samples are heterogeneously distributed in their
length. Nanotechnology advantageously allows for highly defined length distribution
of synthetically engineered fibers that enable for in-depth investigation of this
threshold length. We utilized the ability to prepare silver nanofibers of five
defined length classes to demonstrate a threshold fiber length for acute pleural
inflammation. Nickel nanofibers and carbon nanotubes were then used to strengthen
the relationship between fiber length and pleural inflammation. A method of
intrapleural injection of nanofibers in female C57Bl/6 strain mice was used to
deliver the fiber dose, and we then assessed the acute pleural inflammatory
response. Chest wall sections were examined by light and scanning electron
microscopy to identify areas of lesion; furthermore, cell-nanowires interaction on
the mesothelial surface of the parietal pleura in vivo was investigated. Our
results showed a clear threshold effect, demonstrating that fibers beyond 4 μm in
length are pathogenic to the pleura. The identification of the threshold length for
nanofiber-induced pathogenicity in the pleura has important implications for
understanding the structure-toxicity relationship for asbestos-induced mesothelioma
and consequent risk assessment with the aim to contribute to the engineering of
synthetic nanofibers by the adoption of a benign-by-design approach. © The Author
2012. Published by Oxford University Press on behalf of the Society of Toxicology.
AN - rayyan-553781312
AU - Schinwald, A.
AU - Murphy, F. A.
AU - Prina-Mello, A.
AU - Poland, C. A.
AU - Byrne, F.
AU - Movia, D.
AU - Glass, J. R.
AU - Dickerson, J. C.
AU - Schultz, D. A.
AU - Jeffree, C. E.
AU - Macnee, W.
AU - Donaldson, K.
DO - 10.1093/toxsci/kfs171
IS - 2
KW - Asbestos
Fiber threshold
HARN
Pleural inflammation
Risk assessment
Silver nanowires
Animals
Female
Mesothelioma
Metals
Mice
Mice, Inbred C57BL
Nanofibers
Phagocytosis
Pleurisy
asbestos
carbon nanotube
nanofiber
nanoparticle
nanowire
nickel nanofiber
nickel nanowire
silver nanofiber
silver nanoparticle
silver nanowire
unclassified drug
animal experiment
animal model
animal tissue
article
cell interaction
cell phagocytosis
controlled study
disease course
female
in vivo study
mouse
nanotechnology
nonhuman
particle size
pathogenicity
pleura cavity
pleura mesothelioma
pleurisy
risk assessment
thorax wall
Inflammation
PY - 2012
SP - 461-470
ST - The threshold length for fiber-induced acute pleural inflammation: Shedding
light on the early events in asbestos-induced mesothelioma
T2 - Toxicological Sciences
TI - The threshold length for fiber-induced acute pleural inflammation: Shedding
light on the early events in asbestos-induced mesothelioma
84865349541&doi=10.1093%2ftoxsci
%2fkfs171&partnerID=40&md5=c3d3ee89db6ebb3b3f1b71b2c8950d59
VL - 128
ID - 9256
ER -
TY - JOUR
AB - OBJECTIVES: Bovine and porcine pericardial patches are frequently used in
cardiothoracic and vascular surgery. There are no guidelines recommending the usage
of these patches for particular surgical approaches. However, these 2 materials
supposedly possess different properties. The clinical advantage of porcine compared
with bovine patches remains controversial. In this experimental study, we analysed
the incorporation and vascularization of bovine and porcine pericardial patches
during the initial phase after implantation. METHODS: Bovine and porcine
pericardial patches were implanted into the dorsal skinfold chamber of C57BL/6 mice
(n = 8 per group) to study vascularization and inflammation at the implantation
site using repetitive intravital fluorescence microscopy over a 14-day period. At
the end of the in vivo experiments, CD-31-positive cells were determined to
evaluate the vascularization by immunohistochemistry. Furthermore, cell
proliferation and apoptosis were analysed immunohistochemically. RESULTS: Implanted
bovine patches exhibited an enhanced vascularization, as indicated by a
significantly higher number of CD-31-positive cells and micro-vessels (23.2 +/- 4.3
vs 16.5 +/- 5.8 mm(-2); P=0.001). Furthermore, bovine patches showed a slightly but
not significantly higher functional capillary density. Both patches induced a
moderate leukocytic inflammatory host tissue response, and neither bovine nor
porcine patches significantly affected apoptosis and cell proliferation at the
implantation site. CONCLUSIONS: Bovine and porcine pericardial patches are
similarly suitable for surgery. Bovine patches exhibited an improved
vascularization during the first 14 days after implantation. This may result in a
quicker and improved incorporation into the surrounding tissue compared with
porcine pericardial patches.
AN - rayyan-553781313
AU - Schlachtenberger, G.
AU - Doerr, F.
AU - Brezina, A.
AU - Menghesha, H.
AU - Heldwein, M. B.
AU - Bennink, G.
AU - Menger, M. D.
AU - Moussavian, M.
AU - Hekmat, K.
AU - Wahlers, T.
DO - 10.1093/icvts/ivaa308
IS - 4
PY - 2021
SN - 1569-9293 1569-9285
SP - 638-647
ST - Perigraft reaction and incorporation of porcine and bovine pericardial
patches
T2 - INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY
TI - Perigraft reaction and incorporation of porcine and bovine pericardial
patches
VL - 32
Y2 - 4
ID - 9257
ER -
TY - JOUR
AB - BACKGROUND: The purpose of this preliminary study was to assess the in vivo
performance of synthetic, cotton wool-like nanocomposites consisting of a
biodegradable poly(lactide-co-glycolide) fibrous matrix and containing either
calcium phosphate nanoparticles (PLGA/CaP 60:40) or silver doped CaP nanoparticles
(PLGA/Ag-CaP 60:40). Besides its extraordinary in vitro bioactivity the latter
biomaterial (0.4 wt% total silver concentration) provides additional antimicrobial
properties for treating bone defects exposed to microorganisms. MATERIALS AND
METHODS: Both flexible artificial bone substitutes were implanted into totally 16
epiphyseal and metaphyseal drill hole defects of long bone in sheep and followed
for 8 weeks. Histological and histomorphological analyses were conducted to
evaluate the biocompatibility and bone formation applying a score system. The
influence of silver on the in vivo performance was further investigated. RESULTS:
Semi-quantitative evaluation of histology sections showed for both implant
materials an excellent biocompatibility and bone healing with no resorption in the
adjacent bone. No signs of inflammation were detectable, either macroscopically or
microscopically, as was evident in 5 µm plastic sections by the minimal amount of
inflammatory cells. The fibrous biomaterials enabled bone formation directly in the
centre of the former defect. The area fraction of new bone formation as determined
histomorphometrically after 8 weeks implantation was very similar with 20.5 ± 11.2
% and 22.5 ± 9.2 % for PLGA/CaP and PLGA/Ag-CaP, respectively. CONCLUSIONS: The
cotton wool-like bone substitute material is easily applicable, biocompatible and
might be beneficial in minimal invasive surgery for treating bone defects.
AN - rayyan-553782261
AU - Schneider, O. D.
AU - Mohn, D.
AU - Fuhrer, R.
AU - Klein, K.
AU - Kämpf, K.
AU - Nuss, K. M.
AU - Sidler, M.
AU - Zlinszky, K.
AU - von Rechenberg, B.
AU - Stark, W. J.
DO - 10.2174/1874325001105010063
J2 - Open Orthop J
KW - Sheep
Osteogenesis
LA - eng
N1 - Institute for Chemical and Bioengineering, Department of Chemistry and
Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.
PY - 2011
SP - 63-71
ST - Biocompatibility and Bone Formation of Flexible, Cotton Wool-like
PLGA/Calcium Phosphate Nanocomposites in Sheep
T2 - The open orthopaedics journal
TI - Biocompatibility and Bone Formation of Flexible, Cotton Wool-like
PLGA/Calcium Phosphate Nanocomposites in Sheep
VL - 5
Y2 - 3 y3 - 16
ID - 10171
ER -
TY - JOUR
AB - B1 bradykinin receptors were visualized by using the B1 bradykinin receptor
agonist [3H]des-Arg10-kallidin in receptor autoradiography experiments.
Cryosections were prepared from arterial vessels from a healthy control pig, a pig
with pre-existing inflammation and an animal with experimental sepsis induced by an
infusion of bacterial lipopolysaccharide (LPS). Only diffusely scattered silver
grains with no preference for a distinct tissue structure were detected on
emulsion-coated coverslips above the cryosections from the healthy control animal.
This indicates that under normal circumstances no or only minute amounts of B1
bradykinin receptors are present in these tissues. In contrast, a 3-fold increase
in specific B1 bradykinin receptor binding was observed on both the corresponding
preparations of the sick piglet and of that with experimentally induced sepsis. A
similar enhancement of specific [3H]des-Arg10-kallidin binding occurred in
preparations devoid of endothelium. By comparison with the stained cryosection on
the slide the silver grains showed a preferential distribution above smooth muscle
cells. Taken together our data are consistent with the hypothesis that B1
bradykinin receptors are induced in the muscle layer of large vessels not only
after experimentally-induced sepsis but also in pre-existing inflammatory disease.
AN - rayyan-553782281
AU - Schremmer-Danninger, E.
AU - Offner, A.
AU - Siebeck, M.
AU - Heinz-Erian, P.
AU - Gais, P.
AU - Roscher, A. A.
DO - 10.1016/0162-3109(96)00019-7
IS - 1
J2 - Immunopharmacology
KW - Animals
Aorta, Thoracic/metabolism
Autoradiography
Blood Vessels/*metabolism
Inflammation/*metabolism
Kallidin/analogs & derivatives/metabolism
Pulmonary Artery/metabolism
Receptor, Bradykinin B1
Receptors, Bradykinin/agonists/*metabolism
Sepsis/etiology/metabolism
Swine
Receptors, Bradykinin
Inflammation
Bradykinin
LA - eng
N1 - Department of Surgery, Klinikum Innenstadt, University of Munich, Germany.
PY - 1996
SN - 0162-3109 (Print)
SP - 95-100
ST - Autoradiographic visualization of B1 bradykinin receptors in porcine vascular
tissues in the presence or absence of inflammation
T2 - Immunopharmacology
TI - Autoradiographic visualization of B1 bradykinin receptors in porcine vascular
tissues in the presence or absence of inflammation
VL - 33
Y2 - 6
ID - 10191
ER -
TY - JOUR
AB - The aim of this project was the histopathological and immunofluorescence
evaluation of the cutaneous toxicity of the vesicant chemical compound 2-
chloroethyl-ethyl sulphide (CEES), a yperite simulator, and the effectiveness of
the applied antidote. A complex antidote formula was developed, the treatment
offered 100% protection in case of exposure to 1DL50 chemical vesicant. The
histopathological evaluation showed that the association of the newly developed
antidote with antioxidant and anti-inflammatory actions with the antidotic
formulation, in the form of gel, with regenerating, moisturizing and
epithelializing actions, is beneficial for 0.25-1 LD50 2-chloroethyl-ethyl sulphide
concentrations. Immunofluorescence evaluation of the expression of anti-c-ROS-1 and
anti-PARP-1 antibodies, respectively, highlighted the antidysplastic
reepithelialising and nuclear stabilizing protective effect of the complex antidote
on the skin lesions induced by the studied vesicant compound. The combination of
the two complex curative antidotes (A1 formulated as a solution and A2 formulated
as a hydrogel) developed within the project has superior therapeutic efficacy. It
can guide the therapeutic conduct in case of exposure to vesicant chemicals. ©
2022, Romanian Society for Pharmaceutical Sciences. All rights reserved.
AN - rayyan-553781317
AU - Secară, C. A.
AU - Catrina, A. M.
AU - Voinea, O. C.
AU - Dinu, S. S.
AU - Hîrjău, A. C.
AU - Șerbănescu, L. G.
AU - Serban, D.
AU - Smarandache, G. C.
AU - Haidoiu, C.
AU - Radu, S.
AU - Tudor, C.
AU - Costea, D. O.
AU - Comandasu, M.
AU - Dascalu, A. M.
AU - Păuna, A.
AU - Tudosie, M. S.
DO - 10.31925/farmacia.2022.3.11
IS - 3
KW - 2-chlorethyl-ethyl sulphide
antidote
CEES
toxicity
vesicants
2 chloroethyl ethyl sulphide
acetylcysteine
collagen
dexamethasone
disodium hydrogen phosphate
doxycycline
hyaluronic acid
hydrogel
lanolin
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
nicotinamide nucleotide
potassium chloride
potassium dihydrogen phosphate
sodium chloride
sulfadiazine silver
toxic substance
unclassified drug
acanthosis
animal experiment
animal model
animal tissue
Article
blister
chemical burn
confocal microscopy
controlled study
drug dosage form
drug dosage form comparison
drug efficacy
drug formulation
epithelization
female
histopathology
hyperkeratosis
immunofluorescence
in vivo study
LD50
male
nonhuman
protein expression
skin protection
skin toxicity
Burns, Chemical
Burns
Antidotes
PY - 2022
SP - 456-464
ST - IN VIVO ASSESSMENT OF SKIN CHEMICAL BURNS IN EXPOSURE TO VESICANTS AND THE
EFFICACY OF AN ANTIDOTE FORMULA IN DIFFERENT PHARMACEUTICAL FORMS. AN EXPERIMENTAL
APPROACH
T2 - Farmacia
TI - IN VIVO ASSESSMENT OF SKIN CHEMICAL BURNS IN EXPOSURE TO VESICANTS AND THE
EFFICACY OF AN ANTIDOTE FORMULA IN DIFFERENT PHARMACEUTICAL FORMS. AN EXPERIMENTAL
APPROACH
85133314118&doi=10.31925%2ffarmacia.2022.3.11&partnerID=40&md5=10c16e6b15c788507ad3
2ec34d08e980
VL - 70
ID - 9261
ER -
TY - JOUR
AB - The blood compatibility of AgNPs is of great relevance as it has good
antifungal, antibacterial and anti-inflammatory properties and the toxicological
information of their effects on cells need to be analyzed before using it as drug
carriers in the biomedical field. The present study deals with the synthesis of
AgNPs from an aqueous solution of silver nitrate using Acalypha hispida leaf
extract as the reducing and capping agent. The presence of AgNPs in the reaction
mixture was confirmed by visual observation of color change and subsequently
identified using UV-Visible Spectroscopy. XRD results revealed the crystalline
nature of synthesized AgNPs. The shape and size of particles were characterized by
TEM. These results revealed the elemental status of nanopowder. The components
present in leaf extract were identified by GC-MS and functional groups present in
the sample when treated with silver nitrate were obtained from FT-IR results. The
surface of synthesized AgNPs was modified using four different compounds such as
CTAB, PEG, PEI, and APTMS to evaluate the blood compatibility. The results showed
that 50 mu g/mL CTAB coated AgNPs and 50 and 100 mu g/mL PEG coated AgNPs had non-
hemolytic property and considered as more blood compatible surface modified AgNPs.
This investigation gives an idea of using surface modified AgNPs in the field of
biomedicine and therapeutic applications.
AN - rayyan-553781322
AU - Selvakumar, P.
AU - Sithara, R.
AU - Viveka, K.
AU - Sivashanmugam, P.
DO - 10.1016/j.jphotobiol.2018.03.018
PY - 2018
SN - 1011-1344
SP - 52-61
ST - Green synthesis of silver nanoparticles using leaf extract of Acalypha
hispida and its application in blood compatibility
T2 - JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
TI - Green synthesis of silver nanoparticles using leaf extract of Acalypha
hispida and its application in blood compatibility
VL - 182
Y2 - 5
ID - 9266
ER -
TY - CHAP
AB - Infective diseases have become health threat of a global proportion due to
appearance and spread of microorganisms resistant to majority of therapeutics
currently used for their treatment. Therefore, there is a constant need for
development of new antimicrobial agents, as well as novel therapeutic strategies.
Quinolines and quinolones, isolated from plants, animals, and microorganisms, have
demonstrated numerous biological activities such as antimicrobial, insecticidal,
anti-inflammatory, antiplatelet, and antitumor. For more than two centuries
quinoline/quinolone moiety has been used as a scaffold for drug development and
even today it represents an inexhaustible inspiration for design and development of
novel semi-synthetic or synthetic agents exhibiting broad spectrum of
bioactivities. The structural diversity of synthetized compounds provides high and
selective activity attained through different mechanisms of action, as well as low
toxicity on human cells. This review describes quinoline and quinolone derivatives
with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic
activities with the focus on the last 10 years literature. © Springer Nature
Switzerland AG 2019.
AN - rayyan-553781325
AU - Senerovic, L.
AU - Opsenica, D.
AU - Moric, I.
AU - Aleksic, I.
AU - Spasić, M.
AU - Vasiljevic, B.
DO - 10.1007/5584_2019_428
KW - Anti-parasitics
Anti-virulence activity
Antibiotics
Antifungals
Antivirals
Quinoline/quinolone derivatives
Animals
Antifungal Agents
Antiparasitic Agents
Antiviral Agents
Humans
Quinolines
Quinolones
Virulence
2 quinolone
4 quinolone
8 quinolinol derivative
amodiaquine
antibiotic agent
antifungal agent
antileishmanial agent
antimalarial agent
antiparasitic agent
antitrypanosomal agent
antivirus agent
bedaquiline
besifloxacin
broxyquinoline
chloroquine
ciprofloxacin
levofloxacin
mefloquine
molecular scaffold
moxifloxacin
nalidixic acid
norfloxacin
primaquine
quinine
quinoline
quinoline derivative
sulfadiazine silver
tefenoquine
unclassified drug
unindexed drug
antiinfective agent
animal
antiplatelet activity
drug design
drug development
drug isolation
drug potency
drug selectivity
drug structure
human
insecticidal activity
microorganism
nonhuman
plant
priority journal
drug effect
virulence
Quinolinic Acid
PY - 2020
SP - 37-69
ST - Quinolines and quinolones as antibacterial, antifungal, anti-virulence,
antiviral and anti-parasitic agents
T2 - Advances in Experimental Medicine and Biology
TI - Quinolines and quinolones as antibacterial, antifungal, anti-virulence,
antiviral and anti-parasitic agents
85089126743&doi=10.1007%2f5584_2019_428&partnerID=40&md5=c229e6d5bea3b3ef936f5533e0
5f1261
VL - 1282
ID - 9269
ER -
TY - JOUR
AB - Silver-based biomaterials have been developed in a variety of bactericidal
applications, especially for wound dressings. In this study, silver nanoparticles
(AgNPs) were synthesized in a sodium alginate solution and then the composite
sponge containing AgNPs was prepared from the nanocolloid solution. The alginate-
stabilized AgNPs had the mean negative zeta potential of -52.5mV, suggesting that
the surface charge prevents the nanoparticles from aggregating through
electrostatic repulsion. The alginate-AgNPs composite sponge had a highly enhanced
antimicrobial activity compared to the alginate sponge. In spite of excellent
cytocompatibility of the alginate sponge, the viability of the cell treated with
the alginate-AgNPs composite sponge extract decreased to 86% of the control. The
amount of proinflammatory cytokines released from macrophages treated with the
alginate-AgNPs composite sponge was reduced. For the preparation of AgNPs-embedded
composites, alginate can be a potential candidate stabilizing AgNPs and providing
synergistic antimicrobial and antiinflammatory activities with AgNPs.
AN - rayyan-553782135
AU - Seo, S. Y.
AU - Lee, G. H.
AU - Lee, S. G.
AU - Jung, S. Y.
AU - Lim, J. O.
AU - Choi, J. H.
DO - 10.1016/j.carbpol.2012.05.002
IS - 1
J2 - Carbohydr Polym
KW - Alginates/*chemistry/pharmacology
Animals
Anti-Bacterial Agents/*chemistry/pharmacology
Anti-Inflammatory Agents/*chemistry/pharmacology
Bacterial Infections/drug therapy
Biocompatible Materials/*chemistry/pharmacology
Cell Line
Glucuronic Acid/chemistry/pharmacology
Hexuronic Acids/chemistry/pharmacology
Humans
Macrophages/drug effects/immunology
Metal Nanoparticles/chemistry/ultrastructure
Mice
Nanocomposites/*chemistry/ultrastructure
Silver/*chemistry/pharmacology
Alginates
LA - eng
N1 - Division of Nano & Bio Technology, Daegu Gyungbuk Institute of Science &
Technology, Daegu 711-873, Republic of Korea.; Division of Nano & Bio Technology,
Daegu Gyungbuk Institute of Science & Technology, Daegu 711-873, Republic of
Korea.; Division of Nano & Bio Technology, Daegu Gyungbuk Institute of Science &
Technology, Daegu 711-873, Republic of Korea.; Department of Advanced Organic
Materials Science and Engineering, Kyungpook National University, Daegu 702-701,
Republic of Korea.; Joint Institute for Regenerative Medicine, Kyungpook National
University Hospital, Daegu 700-721, Republic of Korea; Biomedical Research
Institute, School of Medicine, Kyungpook National University Hospital, Daegu 700-
721, Republic of Korea.; Department of Advanced Organic Materials Science and
Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea;
Department of Bio-fibers and materials Science, Kyungpook National University,
Daegu 702-701, Republic of Korea.
PY - 2012
SP - 109-15
ST - Alginate-based composite sponge containing silver nanoparticles synthesized
in situ
T2 - Carbohydrate polymers
TI - Alginate-based composite sponge containing silver nanoparticles synthesized
in situ
VL - 90
Y2 - 9 y3 - 1
ID - 10046
ER -
TY - JOUR
AB - Necroptosis is a regulated cell death that is governed by mixed lineage
kinase domain-like, receptor-interacting serine-threonine kinase 3 and commonly
displays with necrosis morphological characteristics. This study examined the
molecular mechanisms involved in the chemical-induced necroptosis where a
systematic evaluation of experimental studies addressing this issue is missing. We
strictly reviewed all scientific reports related to our search terms including
“necroptosis” or “programmed necrosis”, “environmental chemicals” or “air
pollutants” or “pesticides” or “nanoparticles” and “Medicines” from 2009 to 2019.
Manuscripts that met the objective of this study were included for further
evaluations. Studies showed that several pathological contexts like cancer,
neurodegenerative disorders, and inflammatory diseases were related to necroptosis.
Furthermore, multiple chemical-induced cytotoxic effects, such as DNA damage,
mitochondrial dysregulation, oxidative damage, lipid peroxidation, endoplasmic
reticulum disruption, and inflammation are also associated with necroptosis. The
main environmental exposures that are related to necroptosis are air pollutants
(airborne particulate matter, cadmium, and hydrogen sulfide), nanoparticles (gold,
silver, and silica), pesticides (endosulfan, cypermethrin, chlorpyrifos, and
paraquat), and tobacco smoke. To sum up, air pollutants, pesticides, and
nanoparticles could potentially affect human health via disruption of cell growth
and induction of necroptosis. Understanding the exact molecular pathogenesis of
these environmental chemicals needs further comprehensive research to provide
innovative concepts for the prevention approaches and introduce novel targets for
the amelioration of a range of human health problems. © 2020, Springer-Verlag GmbH
Germany, part of Springer Nature.
AN - rayyan-553781326
AU - Sepand, M. R.
AU - Aliomrani, M.
AU - Hasani-Nourian, Y.
AU - Khalhori, M. R.
AU - Farzaei, M. H.
AU - Sanadgol, N.
DO - 10.1007/s11356-020-09360-5
IS - 30
KW - Apoptosis
Cell death
Environmental chemicals
Nanoparticles
Necroptosis
Humans
Inflammation
Necrosis
Receptor-Interacting Protein Serine-Threonine Kinases
Nicotiana tabacum
receptor interacting protein serine threonine kinase
cancer
cell component
cell organelle
DNA
enzyme
enzyme activity
experimental study
growth
induced response
morphology
nervous system disorder
apoptosis
human
inflammation
necroptosis
necrosis
PY - 2020
SP - 37488-37501
ST - Mechanisms and pathogenesis underlying environmental chemical-induced
necroptosis
T2 - Environmental Science and Pollution Research
TI - Mechanisms and pathogenesis underlying environmental chemical-induced
necroptosis
85088098972&doi=10.1007%2fs11356-020-09360-
5&partnerID=40&md5=9e0883072430bb1a56184b58f71797be
VL - 27
ID - 9270
ER -
TY - JOUR
AB - The combination of silver (Ag) and titanium dioxide (TiO2) nanostructures
offer unique advantages in terms of elimination of infection and enhanced
antibacterial activity with relatively higher biocompatibility and lower
cytotoxicity. Although there have been numerous attempts for the fabrication of
these nanocomposite systems, novel, flexible, low-cost, simple, effective,
reducing, and stabilizing agent-free strategies are highly required for biomedical
applications. Within this context, we report the employment of silver nanostructure
decorated TiO2 nanowires (TiO2 NWs) as an ideal antibacterial agent against
antibiotic-resistant Gram-negative (Escherichia coli) and Gram-positive bacteria
(Staphylococcus aureus). Firstly, TiO2 NWs were fabricated via the hydrothermal
procedure. Afterward, by utilizing the oxidative polymerization of 3,4-
dihydroxyphenyl-L-alanine (L-DOPA), a conformal and thin polymer layer of L-DOPA
(PLDP) was created onto the TiO2 NWs (TiO2/PLDP). Lastly, Ag nanostructures were
deposited onto the TiO2/PLDP (TiO2/PLDP/Ag NP) via simply reduction of silver ions.
Herein, PLDP with its abundant catechol and amine groups played an important role
in the reduction of silver ions and the adsorption of Ag nanostructures with high
affinity and resultant stability. The size, density, and morphology of Ag
nanostructures were manipulated by tuning the initial amount of Ag ions in a well-
controlled manner. The resultant colloidal TiO2/PLDP/Ag composite nanosystem
provided remarkably high and stable antibacterial activity against both antibiotic-
resistant bacteria strains. Minimum Inhibitory Concentration (MIC) values were
found to be 125, 250, and > 500 ppm for high, medium and, low deposition of Ag
nanostructures, respectively. Similarly, Minimum Bactericidal Concentration (MBC)
for these NP systems, MBC values were found to be 250, 500, and > 1000 ppm,
respectively. Also, relatively lower cytotoxicity in human lung healthy (MRC5) and
cancer (A549) cell lines was detected in a dose-dependent manner in comparison to
the citrate-stabilized Ag nanoparticles. The proposed novel TiO2/PLDP/Ag nanosystem
will provide unique opportunities in terms of flexibility, low-cost, simplicity
with reducing, and stabilizing agent-free strategy and be employed in the removal
of biofilms and anti-inflammatory effects.
AN - rayyan-553781327
AU - Serginay, N.
AU - Dizaji, A. N.
AU - Mazlumoglu, H.
AU - Karatas, E.
AU - Yilmaz, A.
AU - Yilmaz, M.
DO - 10.1016/j.colsurfa.2022.128350
KW - RNA, Messenger
Titanium
Polyglactin 910
Poly A-U
Dihydroxyphenylalanine
Poly I-C
PY - 2022
SN - 0927-7757 1873-4359
ST - Antibacterial activity and cytotoxicity of bioinspired poly (L-DOPA)-mediated
silver nanostructure-decorated titanium dioxide nanowires
T2 - COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
TI - Antibacterial activity and cytotoxicity of bioinspired poly (L-DOPA)-mediated
silver nanostructure-decorated titanium dioxide nanowires
VL - 639
Y2 - 4 y3 - 20
ID - 9271
ER -
TY - JOUR
AB - Background and purpose: Despite the widespread use of silver nanoparticles,
there are concerns about their biological effects on the environment and human
health. The aim of this study was to investigate the cytotoxic effect of Ag+
nanoparticles on liver tissue and enzyme activities in NMRI mice. Materials and
methods: In this experimental study, thirty five female NMRI mice were randomly
divided into one control group and four experimental groups (n=7 per group). The
mice in experimental groups were injected with silver nanoparticles at different
concentrations (50, 100, 200, and 400 mg/kg intraperitoneally, every other day).
Blood samples were taken from the inner corners of the eyes of mice and after
separating the serum, liver enzyme activities were analyzed. Then, all animals were
euthanized via cervical dislocation and tissue samples were stained with
hematoxylin and eosin for histopathology evaluation. Statistical analysis was
performed using SAS software. Results: Histopathological examination showed that
different concentrations of Ag+ nanoparticles resulted in mild to severe injury
(necrosis, inflammatory cell infiltration, and vacuolar degeneration) in the liver.
The activity of liver enzymes significantly increased in all groups at 400 mg/kg
concentration compared to that of the control group. Conclusion: Our findings
indicated that exposure to different concentrations of Ag+ nanoparticles can cause
severe damages in the mice liver and increase serum enzyme activities. © 2015,
Mazandaran University of Medical Sciences. All rights reserved.
AN - rayyan-553781330
AU - Seyedalipour, B.
AU - Arefifar, A.
AU - Khanbabaee, R.
AU - Hoseini, S. M.
IS - 124
KW - Inflammatory cell infiltration
Necrosis
NMRI mice
Transaminase
silver nanoparticle
animal experiment
animal model
animal tissue
Article
cell infiltration
controlled study
cytotoxicity
enzyme activity
female
histopathology
liver toxicity
mouse
necrosis
NMRI mouse
nonhuman
Mice
PY - 2015
SP - 183-193
ST - Toxicity of silver nanoparticles on ALT, AST, ALP and histopathological
changes in NMRI mice
T2 - Journal of Mazandaran University of Medical Sciences
TI - Toxicity of silver nanoparticles on ALT, AST, ALP and histopathological
changes in NMRI mice
85008397796&partnerID=40&md5=81794fd191d1d7655da545cf00e6f850
VL - 25
ID - 9273
ER -
TY - JOUR
AB - The purpose of current study was green synthesis of silver nanoparticles
(AgNPs) from seeds and wild Silybum plants in comparison with their respective
extracts followed by characterization and biological potency. The biologically
synthesized AgNPs were subjected to characterization using techniques like XRD,
FTIR, TEM, HPLC and SPE. Highly crystalline and stable NPs were obtained using
Silybum wild plant (NP1) and seeds (NP3) with size range between 18.12 and 13.20 nm
respectively. The synthesized NPs and their respective extracts revealed a vast
range of biological applications showing antibacterial, antioxidant, anti-
inflammatory, cytotoxic and anti-aging potencies. The highest antioxidant activity
(478.23 ± 1.9 μM, 176.91 ± 1.3 μM, 83.5 ± 1.6% μgAAE/mg, 156.32 ± 0.6 μgAAE/mg) for
ABTS, FRAP, FRSA, TRP respectively was shown by seed extract (NP4) followed by
highest value of (117.35 ± 0.9 μgAAE/mg) for TAC by wild extract (NP2). The highest
antifungal activity (13 mm ± 0.76) against Candida albicans was shown by NP3 while
antibacterial activity of (6 mm against Klebsiella pneumonia) was shown by NP3 and
NP4. The highest anti-inflammatory activity (38.56 ± 1.29 against COX1) was shown
by NP2. Similarly, the high value of (48.89 ± 1.34 against Pentosidine-Like AGEs)
was shown by NP4. Also, the high anti-diabetic activity (38.74 ± 1.09 against α-
amylase) was shown by NP4. The extracts and the synthesized NPs have shown activity
against hepato-cellular carcinoma (HepG2) human cells. The HPLC analysis revealed
that the highest value of silymarin component (silybin B 2289 mg/g DW) was found
for NP4. Silydianin is responsible for capping. Among the green synthesized AgNPs
and the extracts used, the effect of NP4 was most promising for further use.
AN - rayyan-553781804
AU - Shah, M.
AU - Nawaz, S.
AU - Jan, H.
AU - Uddin, N.
AU - Ali, A.
AU - Anjum, S.
AU - Giglioli-Guivarc'h, N.
AU - Hano, C.
AU - Abbasi, B. H.
DO - 10.1016/j.msec.2020.110889
J2 - Mater Sci Eng C Mater Biol Appl
KW - Amylases/antagonists & inhibitors/metabolism
Animals
Anti-Infective Agents/chemical synthesis/chemistry/pharmacology
Anti-Inflammatory Agents/chemical synthesis/chemistry/pharmacology
Antidiuretic Agents/chemistry/metabolism
Antioxidants/chemistry
Cattle
Cyclooxygenase 1/metabolism
Hep G2 Cells
Humans
Klebsiella pneumoniae/drug effects
Milk Thistle/*chemistry/metabolism
Plant Extracts/*chemistry
Seeds/chemistry/metabolism
Silver/*chemistry
Milk Thistle
Tocopherols
Ambulatory Care Facilities
LA - eng
N1 - Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320,
Pakistan.; Department of Microbiology, Quaid-i-Azam University, Islamabad 45320,
Pakistan.; Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320,
Pakistan.; Department of Chemistry, Quaid-i-Azam University, Islamabad 45320,
Pakistan.; Key State Laboratory of Virology, Wuhan Institute of Virology, Chinese
Academy of Sciences, 430072 Wuhan, China.; Department of Biotechnology, Kinnaird
College for Women, Lahore 54000, Pakistan.; EA2106 Biomolécules et Biotechnologies
Végétales, Université de Tours, 37000 Tours, France; COSM'ACTIFS, Bioactifs et
Cosmétiques, CNRS GDR3711, 45067 Orléans CEDEX 2, France.; COSM'ACTIFS, Bioactifs
et Cosmétiques, CNRS GDR3711, 45067 Orléans CEDEX 2, France; Laboratoire de
Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université
d'Orléans, 45067 Orléans CEDEX 2, France.; Department of Biotechnology, Quaid-i-
Azam University, Islamabad 45320, Pakistan; EA2106 Biomolécules et Biotechnologies
Végétales, Université de Tours, 37000 Tours, France; COSM'ACTIFS, Bioactifs et
Cosmétiques, CNRS GDR3711, 45067 Orléans CEDEX 2, France; Laboratoire de Biologie
des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d'Orléans,
45067 Orléans CEDEX 2, France. Electronic address: bhabbasi@qau.edu.pk.
PY - 2020
SP - 110889
ST - Synthesis of bio-mediated silver nanoparticles from Silybum marianum and
their biological and clinical activities
T2 - Materials science & engineering. C, Materials for biological applications
TI - Synthesis of bio-mediated silver nanoparticles from Silybum marianum and
their biological and clinical activities
VL - 112
Y2 - 7
ID - 9734
ER -
TY - JOUR
AB - Recently, we have published a pioneering work on green biosynthesis and
complete characterization of gold and core shell silver-gold nanoparticles (AuNPs
and Ag@AuNPs). Herein, the so obtained nanoparticles are assessed for their
antidiabetic activity in streptozotocin-induced diabetic rats. Thus, sixty-four
male albino rats were divided into eight groups: control untreated; diabetic rats;
diabetic rats received standard drug; diabetic rats received carrier only; diabetic
rats received 0.5 ml AuNPs; diabetic rats received 1 ml AuNPs; diabetic rats
received 0.5 ml Ag@AuNPs and diabetic rats received 1 ml Ag@AuNPs for twenty-one
days. Results revealed that diabetic rats treated with AuNPs or Ag@AuNPs restored
normal glucose level. In particular, Ag@AuNPs was found to significantly induce a
reduction in blood glucose and restore both the high serum insulin level and
glucokinase activity compared to the control normal rats. The results obtained
disclose the effectual role of Ag@AuNPs in reducing the lipid profile, an anti-
inflammatory effect in diabetic rats assessed using inflammatory markers IL-α and
C-reactive protein (CRP). Histopathological examination of diabetic rats signifies
distortion in the arrangement of cells around the central vein, inflammatory cells,
pyknotic and apoptotic nuclei. Kidney of diabetic rat appears with vacuolation and
pyknotic nuclei of some tubules. On the other hand, the liver of diabetic rat
treated with Ag@AuNPs displayed normal hepatic cells with only few necrosis of
hepatocytes. Ag@AuNPs restored the increased number of caspase-3 stained cells in
the liver and kidney tissue in diabetic rats. In conclusion, Ag@AuNPs was observed
to improve diabetic condition by limiting prolonged inflammation, suppressing
oxidative stress and elevating the antioxidant defense system in diabetic rats
which subsequently evoke the potential impact of AuNPs as a cost effective
therapeutic cure in diabetic treatments and its complications. © 2016 Elsevier
Masson SAS
AN - rayyan-553781333
AU - Shaheen, T. I.
AU - El-Naggar, M. E.
AU - Hussein, J. S.
AU - El-Bana, M.
AU - Emara, E.
AU - El-Khayat, Z.
AU - Fouda, M. M. G.
AU - Ebaid, H.
AU - Hebeish, A.
DO - 10.1016/j.biopha.2016.07.052
KW - Blood glucose
Core shell silver–gold nanoparticles
Diabetic
Gold nanoparticles
Animals
Antioxidants
Blood Glucose
Caspase 3
Fasting
Gold
Hypoglycemic Agents
Inflammation
Insulin
Kidney
Lipids
Liver
Male
Metal Nanoparticles
Nitric Oxide
Oxidation-Reduction
Rats
Silver
Spectrophotometry, Ultraviolet
Streptozocin
antidiabetic agent
antilipemic agent
antioxidant
C reactive protein
caspase 3
glucokinase
glucose
gold nanoparticle
insulin
silver nanoparticle
triacylglycerol
glucose blood level
gold
lipid
metal nanoparticle
nitric oxide
silver
streptozocin
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
cardiovascular risk
controlled study
drug potency
enzyme inhibition
green chemistry
immunoreactivity
in vivo study
insulin blood level
liver necrosis
lymphocytic infiltration
male
nanomedicine
nonhuman
oxidative stress
particle size
priority journal
rat
triacylglycerol blood level
animal
blood
chemistry
diet restriction
drug effects
inflammation
kidney
liver
metabolism
pathology
ultrastructure
PY - 2016
SP - 865-875
ST - Antidiabetic assessment; in vivo study of gold and core-shell silver-gold
nanoparticles on streptozotocin-induced diabetic rats
T2 - Biomedicine and Pharmacotherapy
TI - Antidiabetic assessment; in vivo study of gold and core-shell silver-gold
nanoparticles on streptozotocin-induced diabetic rats
84982275673&doi=10.1016%2fj.biopha.2016.07.052&partnerID=40&md5=3633d744d17ba0f8253
1b41da4e7a50a
VL - 83
ID - 9276
ER -
TY - JOUR
AB - Tissue engineering techniques are continuously evolving towards providing
better microenvironment along with therapeutic potential to address the skin tissue
defects. Factors such as microbial infections, presence of excessive free radicals
and depletion in antioxidant based scavenging systems pose serious challenges by
prolonging inflammation and delaying the repair process. Incorporation of bioactive
molecules in polymer based biomimetic scaffolds may present new vistas for handling
chronic wounds. In this study, chitosan/collagen scaffolds incorporating 0.5, 1 and
2% (w/w) silymarin (CS-CO-SM) were synthesized and studied for their
biocompatibility, in vitro release kinetics and anti-oxidant activity. The release
kinetics of silymarin from the CS-CO-SM scaffold showed an initial burst followed
by sustained release. The scaffolds were biocompatible and supported the recovery
of COS-7 cells from UV induced oxidative stress. Further the CS-CO-SM (2) scaffolds
were used to fabricate a bi-layer scaffold by layer upon layer arrangement with CS-
Ag3 (3% Ag, w/w). The Ag was incorporated to impart antimicrobial property to the
scaffold. The in vivo studies on bi-layer scaffolds were carried out in Wistar rat
models at 3, 7 and 10 days post injury and the skin excisions were studied for
wound contraction, histology (H&E staining), and lipid peroxidation. The bi-layer
scaffold accelerated the process of wound healing with no inflammatory cells,
proliferation of fibroblast, neovascularization and collagen deposition. By day 10
post transplantation of the scaffold, the skin had a structure similar to normal
skin with complete re-epithelization. This bi-layer scaffold with antioxidant and
antimicrobial properties promotes wound healing and is proposed as a potential
tissue engineering material for managing chronic wounds. [Figure not available: see
fulltext.]. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
AN - rayyan-553781334
AU - Shaik, M. M.
AU - Dapkekar, A.
AU - Rajwade, J. M.
AU - Jadhav, S. H.
AU - Kowshik, M.
DO - 10.1007/s10856-018-6212-8
IS - 1
KW - Animals
Antioxidants
Cercopithecus aethiops
COS Cells
Lipid Peroxidation
Oxidative Stress
Random Allocation
Rats
Rats, Wistar
Tissue Engineering
Tissue Scaffolds
Wound Healing
antiinfective agent
antioxidant
chitosan
collagen
silver
silymarin
animal experiment
animal model
animal tissue
Article
biocompatibility
controlled study
excision
in vitro study
in vivo study
infection
nonhuman
oxidative stress
priority journal
rat
sustained drug release
wound
wound contraction
wound healing
animal
bacterial infection
Chlorocebus aethiops
CV-1 cell line
drug effect
lipid peroxidation
procedures
randomization
tissue engineering
tissue scaffold
Wistar rat
PY - 2019
ST - Antioxidant-antibacterial containing bi-layer scaffolds as potential
candidates for management of oxidative stress and infections in wound healing
T2 - Journal of Materials Science: Materials in Medicine
TI - Antioxidant-antibacterial containing bi-layer scaffolds as potential
candidates for management of oxidative stress and infections in wound healing
85059896119&doi=10.1007%2fs10856-018-6212-
8&partnerID=40&md5=dddcdd66f4373d7e688c7557c233fd0a
VL - 30
ID - 9277
ER -
TY - JOUR
AB - How to avoid the microenvironment limitations in the therapeutic process of
pressure ulcers is still challenging. The development of a functional gel can kill
bacteria and scavenge reactive oxygen species (ROS), which is urgently required in
the therapeutic process of pressure ulcers. Herein, an in situ sprayed gel is
developed with silver nanoparticles (AgNPs) and polydopamine (PDA) NPs (APG) to
obviate microenvironment restrictions in treating pressure ulcers. The gel is
constructed by spraying sodium alginate solution and CaCl2 solution. AgNPs serve as
an antibacterial agent in the formed gel, which can effectively cause bacterial
inactivation and show more than 5 log (>99.999%) bacterial killing efficiency
against methicillin-resistant S. aureus (MRSA), Staphylococcus aureus (S. aureus),
and Escherichia coli (E. coli) in vitro. Meanwhile, PDA NPs serve as the
antioxidative agent in the formed gel, which can facilitate the elimination of ROS
to address the high ROS problem in wound microenvironment. Based on these features,
it is demonstrated through cell and animal experiments that the AgNPs and PDA NPs
incorporated gel can realize the effective treatment of MRSA-infected and hydrogen
peroxide (H2O2)-sensitized pressure ulcers. It is believed that the designed system
by a simple spray-coating approach can provide a new therapeutic strategy in
biomedical areas. © 2023 Wiley-VCH GmbH.
AN - rayyan-553781336
AU - Shan, J.
AU - Li, X.
AU - Huang, Z.
AU - Kong, B.
AU - Wang, H.
AU - Ren, L.
DO - 10.1002/mabi.202300006
IS - 5
KW - bacteria
gels
microenvironments
pressure ulcers
ROS
Animals
Escherichia coli
Metal Nanoparticles
Pressure Ulcer
Silver
Chlorine compounds
Diseases
Silver compounds
Sodium alginate
alginic acid
calcium chloride
dermatological agent
dopamine
gel
hydrogen peroxide
silver nanoparticle
silver polydopamine nanoparticle
unclassified drug
antiinfective agent
metal nanoparticle
silver
Alginate solutions
Bacterial inactivation
Difunctional
Methicillin
Microenvironments
Polydopamine
Pressure ulcers
S. aureus
Scavenge reactive oxygen species
animal experiment
animal model
animal tissue
Article
biocompatibility
controlled study
decubitus
in vitro study
in vivo study
inflammatory cell
mouse
nonhuman
scar
spray coating
wound
wound care
wound healing
animal
Gels
PY - 2023
ST - In Situ Sprayed Difunctional Gel Avoiding Microenvironments Limitations to
Treat Pressure Ulcers
T2 - Macromolecular Bioscience
TI - In Situ Sprayed Difunctional Gel Avoiding Microenvironments Limitations to
Treat Pressure Ulcers
85151617645&doi=10.1002%2fmabi.202300006&partnerID=40&md5=224092ea2e0ef109c53756318
1003ba6
VL - 23
ID - 9278
ER -
TY - JOUR
AB - Inflammation is one of the basic pathophysiologically important components in
many life-threatening diseases. Metallic nanoparticles play a crucial role in
biomedical applications. The present study was aimed at investigating the
ameliorative effect of biosynthesized silver nanoparticles (ScAgNPs) using Salvia
coccinea leaf extracts and characterizing them using physical and chemical methods,
followed by evaluation of their cytotoxic, anti-oxidant, and anti-inflammatory
potentials in monocytic THP-1 cells. Luciferase reporter assays and qRT-PCRs were
used for gene expression studies. As oxidative stress and inflammation are mutually
induced by each other, inhibiting oxidative stress could subsequently lead to
inhibition of inflammation. Spherical-shaped ScAgNPs with 24 nm average size were
successfully synthesized. The DCF staining technique, in addition to DPPH and
reducing power activity assays, showed that 100-400 μg/mL concentration of ScAgNPs
decreased oxidative stress significantly, induced by high glucose, in THP-1 cells.
Anti-inflammatory effects of ScAgNPs have corroborated inhibition of high-glucose-
induced oxidative stress-sensitive transcription factor NF-κB-driven transcription
of proinflammatory COX-2, MCP-1, IP-10, IL-17E, and IL-6 promoters significant in
high-glucose-grown THP-1 cells, consistent with promoter inhibition, and the
corresponding mRNA expression levels were also decreased, suggesting that ScAgNPs
could be a potential anti-inflammatory agent, which could efficiently inhibit
inflammation in THP-1 cells. Our initial in vitro studies suggested that ScAgNPs
could serve as therapeutic candidates to alleviate inflammatory diseases by
inhibiting oxidative stress and inflammation.
AN - rayyan-553781842
AU - Shanmugam, G.
AU - Sundaramoorthy, A.
AU - Shanmugam, N.
DO - 10.1021/acsabm.1c00963
IS - 12
J2 - ACS Appl Bio Mater
KW - Anti-Inflammatory Agents/pharmacology
Glucose
Humans
*Metal Nanoparticles/therapeutic use
Plant Extracts/pharmacology
*Salvia
Silver/pharmacology
THP-1 Cells
Humanities
Humanism
Monocytes
LA - eng
N1 - Department of Biomedical Science, Bharathidasan University, Tiruchirappalli
620 024, Tamil Nadu, India.; Department of Biomedical Science, Bharathidasan
University, Tiruchirappalli 620 024, Tamil Nadu, India.; Department of Biomedical
Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
PY - 2021
SP - 8433-8442
ST - Biosynthesis of Silver Nanoparticles from Leaf Extract of Salvia coccinea and
Its Effects of Anti-inflammatory Potential in Human Monocytic THP-1 Cells
T2 - ACS applied bio materials
TI - Biosynthesis of Silver Nanoparticles from Leaf Extract of Salvia coccinea and
Its Effects of Anti-inflammatory Potential in Human Monocytic THP-1 Cells
VL - 4
Y2 - 12 y3 - 20
ID - 9769
ER -
TY - JOUR
AB - Nanoparticle (NP) association with macromolecules in a physiological
environment forms a biocorona (BC), which alters NP distribution, activity, and
toxicity. While BC formation is dependent on NP physicochemical properties, little
information exists on the influence of the physiological environment. Obese
individuals and those with cardiovascular disease exist with altered serum
chemistry, which is expected to influence BC formation and NP toxicity. We
hypothesize that a BC formed on NPs following incubation in hyperlipidemic serum
will result in altered NP-BC protein content, cellular association, and toxicity
compared to normal serum conditions. We utilized Fe3O4 NPs, which are being
developed as MRI contrast and tumor targeting agents to test our hypothesis. We
used rat aortic endothelial cells (RAECs) within a dynamic flow in vitro exposure
system to more accurately depict the in vivo environment. A BC was formed on 20 nm
PVP-suspended Fe3O4 NPs following incubation in water, 10% normal or hyperlipidemic
rat serum. Addition of BCs resulted in increased hydrodynamic size and decreased
surface charge. More cholesterol associated with Fe3O4 NPs after incubation in
hyperlipidemic as compared with normal serum. Using quantitative proteomics, we
identified unique differences in BC protein components between the 2 serum types.
Under flow conditions, formation of a BC from both serum types reduced RAECs
association of Fe3O4 NPs. Addition of BCs was found to exacerbate RAECs
inflammatory gene responses to Fe3O4 NPs (Fe3O4-hyperlipidemic > Fe3O4-normal >
Fe3O4) including increased expression of IL-6, TNF-alpha, Cxcl-2, VCAM-1, and ICAM-
1. Overall, these findings demonstrate that disease-induced variations in
physiological environments have a significant impact NP-BC formation, cellular
association, and cell response.
AN - rayyan-553781342
AU - Fritz, K. S.
AU - Raghavendra, A. J.
AU - Podila, R.
AU - Persaud, I.
AU - Brown, J. M.
DO - 10.1093/toxsci/kfw097
IS - 2
PY - 2016
SN - 1096-6080 1096-0929
SP - 406-416
ST - Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and
the Toxicological Consequences
T2 - TOXICOLOGICAL SCIENCES
TI - Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and
the Toxicological Consequences
VL - 152
Y2 - 8
ID - 9284
ER -
TY - JOUR
AB - Human inhalation exposures to manufactured nanoparticles (NP) and airborne
ultrafine particles (UFP) continues to increase in both occupational and
environmental settings. UFP exposures have been associated with increased
cardiovascular mortality and morbidity, while ongoing research supports adverse
systemic and cardiovascular health effects after NP exposures. Adverse
cardiovascular health effects include alterations in heart rate variability,
hypertension, thrombosis, arrhythmias, increased myocardial infarction, and
atherosclerosis. Exactly how UFP and NP cause these negative cardiovascular effects
is poorly understood, however a variety of mediators and mechanisms have been
proposed. UFP and NP, as well as their soluble components, are known to
systemically translocate from the lung. Translocated particles could mediate
cardiovascular toxicity through direct interactions with the vasculature, blood,
and heart. Recent study suggests that sensory nerve stimulation within the lung may
also contribute to UFP- and NP-induced acute cardiovascular alterations. Activation
of sensory nerves, such as C-fibers, within the lung may result in altered cardiac
rhythm and function. Lastly, release of pulmonary-derived mediators into systemic
circulation has been proposed to facilitate cardiovascular effects. In general,
these proposed pulmonary-derived mediators include proinflammatory cytokines,
oxidatively modified macromolecules, vasoactive proteins, and prothrombotic
factors. These pulmonary-derived mediators have been postulated to contribute to
the subsequent prothrombotic, atherogenic, and inflammatory effects after exposure.
This review will evaluate the potential contribution of individual mediators and
mechanisms in facilitating cardiopulmonary toxicity following inhalation of UFP and
NP. Lastly, we will appraise the literature and propose a hypothesis regarding the
possible role of mast cells in contributing to these systemic effects. © 2012
Informa Healthcare USA, Inc.
AN - rayyan-553781343
AU - Kodavanti, U. P.
AU - Brown, J. M.
DO - 10.3109/08958378.2012.668229
IS - 5
KW - IL-33
LOX-1
Mast cell
Particulate matter
RAGE
advanced glycation end product receptor
beta adrenergic receptor blocking agent
carbon nanotube
cerium oxide
chemokine
cytokine
growth factor
lipid
nanoparticle
polystyrene
silver
titanium dioxide
atherogenesis
autonomic nervous system
cardiopulmonary toxicity
cardiotoxicity
cytokine release
endothelium cell
heart function
heart infarction
heart muscle cell
heart rate variability
heart rhythm
human
lung toxicity
mast cell
mediator release
nerve fiber
nonhuman
oxidative stress
particle size
priority journal
review
sensory nerve
thrombosis
ultrafine particle
Mast Cells
PY - 2012
SP - 320-339
ST - Manufactured and airborne nanoparticle cardiopulmonary interactions: A review
of mechanisms and the possible contribution of mast cells
T2 - Inhalation Toxicology
TI - Manufactured and airborne nanoparticle cardiopulmonary interactions: A review
of mechanisms and the possible contribution of mast cells
84859617027&doi=10.3109%2f08958378.2012.668229&partnerID=40&md5=cb980328178049f8620
3cd9f13c50208
VL - 24
ID - 9285
ER -
TY - JOUR
AB - Objective: To explore the therapeutic effect and mechanism of Qiling Tongluo
prescription against idiopathic membranous nephropathy (IMN) in rats based on Toll-
like receptor 4/myeloid differentiation factor 88/nuclear transcription factor-κB
(TLR4/MyD88/NF-κB) signaling pathway. Method: Sixty male SD rats were randomly
divided into the normal group, model group, benazepril hydrochloride (10 mg·kg-1)
group, and low-, medium-, and high-dose (6.48, 12.95, and 25.9 g·kg-1) Qiling
Tongluo prescription groups. The IMN rat model was established by injection of
cationized bovine serum albumin (C-BSA) into the tail vein. After the model was
successfully prepared, the rats were gavaged with the corresponding drugs, once a
day, for four consecutive weeks. After the treatment, the pathological changes in
rat kidneys were observed by hematoxylineosin (HE) staining, Masson staining, and
periodic acid-silver metheramine (PASM) staining, followed by the detection of 24 h
urinary total protein (24 h UTP), plasma albumin (ALB), total serum protein (TP),
serum creatinine (SCr), urea nitrogen (BUN), and uric acid (UA) levels. The levels
of interleukin-1β (IL-1β) and interleukin-6 (IL-6) were determined by enzyme-linked
immunosorbent assay (ELISA), and the mRNA and protein expression levels of TLR4,
MyD88, and NF-κB in the kidney tissue were assayed by real-time fluorescent
quantitative polymerase chain reaction (Real-time PCR), immunohistochemistry (IHC),
and Western blot. Result: Compared with the normal group, the model group exhibited
elevated 24 h UTP and serum SCr, BUN, UA, IL-1β, and IL-6 (P<0.05, P<0.01),
decreased ALB and TP (P<0.01), up-regulated TLR4, MyD88, and NF-κB p65 mRNA and
protein expression in kidney tissue (P<0.05, P<0.01), obvious inflammation,
disordered glomerular structure with enlarged volume, irregularly thickened
basement membrane, inflammatory cell infiltration in the renal interstitium,
reduced renal tubular epithelial cells due to shedding and apoptosis, and some
vacuolar degeneration. Compared with the model group, benazepril hydrochloride and
Qiling Tongluo prescription at the high dose remarkably lowered the serum SCr and
UA (P<0.05) and increased ALB and TP (P<0.05). Benazepril hydrochloride and Qiling
Tongluo prescription at the low, medium, and high doses down-regulated the 24 h
UTP, serum IL-1β and IL-6 levels, and renal TLR4, MyD88, and NF-κB p65 mRNA and
protein expression to varying degrees (P<0.05, P<0.01), alleviated IMN inflammatory
reaction, glomerular swelling, and volume increase, slightly dilated glomerular
capillaries, proliferated mesangial matrix, and relieved pathological and
morphological damages in rat kidney, with inflammatory cell infiltration
occasionally observed. Conclusion: Qiling Tongluo prescription may reduce the
release and expression of inflammatory factors by regulating the TLR4/MyD88/NF-κB
signaling pathway to inhibit the inflammatory response in IMN rats, ameliorate
proteinuria and kidney damage, and protect kidney function. © 2022, China Academy
of Chinese Medical Sciences Institute of Chinese Materia Medica. All rights
reserved.
AN - rayyan-553781346
AU - Shao, J. B.
AU - Wang, X.
AU - Xu, H. Z.
AU - Gao, F.
AU - Zhang, Y. Q.
AU - Zhang, Y. H.
AU - Yuan, M. H.
AU - Pan, L.
DO - 10.13422/j.cnki.syfjx.20220391
IS - 4
KW - Idiopathic membranous nephropathy (IMN)
Myeloid differentiation factor 88 (MyD88)
Nuclear transcription factor-κB (NF-κB)
Qiling Tongluo prescription
Toll-like receptor 4 (TLR4)
albumin
benazepril
Chinese drug
creatinine
interleukin 1beta
interleukin 6
nitrogen
qiling tongluo
unclassified drug
uric acid
animal experiment
animal model
animal tissue
apoptosis
Article
cell infiltration
Chinese medicine
controlled study
drug activity
drug efficacy
drug megadose
gene expression
inflammation
kidney injury
kidney tissue
low drug dose
male
membranous glomerulonephritis
morphological trait
NF kB signaling
nonhuman
prescription
protein expression
rat
Western blotting
Signal Transduction
Prescriptions
Rats
Glomerulonephritis, Membranous
PY - 2022
SP - 100-108
ST - Qiling Tongluo Prescription Improves Renal Injury in Rats with Idiopathic
Membranous Nephropathy by Inhibiting TLR4/MyD88/NF-κB Signaling Pathway
T2 - Chinese Journal of Experimental Traditional Medical Formulae
TI - Qiling Tongluo Prescription Improves Renal Injury in Rats with Idiopathic
Membranous Nephropathy by Inhibiting TLR4/MyD88/NF-κB Signaling Pathway
85131522671&doi=10.13422%2fj.cnki.syfjx.20220391&partnerID=40&md5=69f4638cc932b609e
b95e25d97f4a298
VL - 28
ID - 9288
ER -
TY - JOUR
AB - Various strategies have been explored to prevent pin tract infections (PTI),
including the use of antibacterial sleeves. However, an ideal animal model to
evaluate the efficacy of antibacterial strategies is still lacking. This study
aimed to construct an animal model with a consistent induction of infection after
bacterial challenge. Further, the efficacy of silver and chlorhexidine loaded
chitosan sleeves was evaluated to prevent PTI around a percutaneous implant.
Titanium pins wrapped with sleeves were implanted in anterior lateral rabbit tibia.
After 2 weeks, Staphylococcus aureus suspensions (1 × 10(6) CFU) were injected weekly
to the exit site, and the clinical infection status was recorded. After 6 weeks,
all rabbits were euthanized to evaluate the bacterial colonization
microbiologically and histomorphometrically. Results showed that the implant screw
bilaterally penetrated the tibia and kept the implant stable. A rod length of twice
the thickness of the soft-tissue layer was necessary to maintain the percutaneous
penetration of the implants. A 100% infection rate was obtained by the bacterial
inoculation. Silver loaded sleeves reduced significantly the bacterial density and
reduced the inflammatory symptoms of the percutaneous pin tract. However, the
addition of chlorhexidine to the sleeves had no added value in terms of further
reduction of bacteria and inflammation. In conclusion, a consistent animal model
was designed to evaluate strategies to prevent PTI. In addition, the use of silver
loaded chitosan sleeves can be pursued for further (pre-)clinical exploration for
the prevention of PTI. STATEMENT OF SIGNIFICANCE: This study constructed a
bacterial challenged percutaneous rabbit tibia model to evaluate the potential of
antibacterial strategies for the prevention of pin tract infections. The model was
applied to evaluate a silver and chlorhexidine loaded membranes as an antibacterial
sleeve. Our results demonstrate that the rabbit tibia model is suitable to evaluate
antibacterial strategies for the prevention of pin tract infection as evidenced by
the stable, bone fixed percutaneous implant and a 100% infection rate of the
percutaneous pin tract. Silver loaded sleeves can lower the bacterial density of
the percutaneous pin tract, but the addition of chlorhexidine to the silver-loaded
sleeves does not contribute to an enhanced antibacterial effect. Such experiments
are of considerable interest to those in the research community, industry, and
clinicians involved the occurrence of infection of skin penetrating medical
devices.
AN - rayyan-553782357
AU - Shao, J.
AU - Wang, B.
AU - Bartels, C. J. M.
AU - Bronkhorst, E. M.
AU - Jansen, J. A.
AU - Walboomers, X. F.
AU - Yang, F.
DO - 10.1016/j.actbio.2018.10.021
J2 - Acta Biomater
KW - Animals
*Anti-Bacterial Agents/chemistry/pharmacology
*Chitosan/chemistry/pharmacology
*Chlorhexidine/chemistry/pharmacology
*Coated Materials, Biocompatible/chemistry/pharmacology
Rabbits
*Silver/chemistry/pharmacology
*Staphylococcal Infections/drug therapy/metabolism/pathology
Staphylococcus aureus/*growth & development
*Surgical Wound Infection/metabolism/pathology/prevention & control
*Tibia/metabolism/microbiology/pathology
LA - eng
N1 - Department of Biomaterials, Radboud University Medical Centre, Nijmegen, The
Netherlands. Electronic address: Jinlong.Shao@radboudumc.nl.; Department of
Biomaterials, Radboud University Medical Centre, Nijmegen, The Netherlands.
Electronic address: Bing.Wang@radboudumc.nl.; Department of Medical Microbiology,
Radboud University Medical Centre, Nijmegen, The Netherlands. Electronic address:
CJM.Bartels@radboudumc.nl.; Department of Biomaterials, Radboud University Medical
Centre, Nijmegen, The Netherlands. Electronic address:
Ewald.Bronkhorst@radboudumc.nl.; Department of Biomaterials, Radboud University
Medical Centre, Nijmegen, The Netherlands. Electronic address:
John.Jansen@radboudumc.nl.; Department of Biomaterials, Radboud University Medical
Centre, Nijmegen, The Netherlands. Electronic address:
Frank.Walboomers@radboudumc.nl.; Department of Biomaterials, Radboud University
Medical Centre, Nijmegen, The Netherlands. Electronic address:
Fang.Yang@radboudumc.nl.
PY - 2018
SP - 102-110
ST - Chitosan-based sleeves loaded with silver and chlorhexidine in a percutaneous
rabbit tibia model with a repeated bacterial challenge
T2 - Acta biomaterialia
TI - Chitosan-based sleeves loaded with silver and chlorhexidine in a percutaneous
rabbit tibia model with a repeated bacterial challenge
VL - 82
Y2 - 12
ID - 10265
ER -
TY - JOUR
AB - Reductive evolution has endowed Mycobacterium tuberculosis (M. tb) with
moonlighting in protein functions. We demonstrate that RipA (Rv1477), a
peptidoglycan hydrolase, activates the NF kappa B signaling pathway and elicits the
production of pro-inflammatory cytokines, TNF-alpha, IL-6, and IL-12, through the
activation of an innate immune-receptor, toll-like receptor (TLR)4. RipA also
induces an enhanced expression of macrophage activation markers MHC-II, CD80, and
CD86, suggestive of M1 polarization. RipA harbors LC3 (Microtubule-associated
protein 1A/1B-light chain 3) motifs known to be involved in autophagy regulation
and indeed alters the levels of autophagy markers LC3BII and P62/SQSTM1
(Sequestosome-1), along with an increase in the ratio of P62/Beclin1, a hallmark of
autophagy inhibition. The use of pharmacological agents, rapamycin and bafilomycin
A1, reveals that RipA activates PI3K-AKT-mTORC1 signaling cascade that ultimately
culminates in the inhibition of autophagy initiating kinase ULK1 (Unc-51 like
autophagy activating kinase). This inhibition of autophagy translates into
efficient intracellular survival, within macrophages, of recombinant Mycobacterium
smegmatis expressing M. tb RipA. RipA, which also localizes into mitochondria,
inhibits the production of oxidative phosphorylation enzymes to promote a Warburg-
like phenotype in macrophages that favors bacterial replication. Furthermore, RipA
also inhibited caspase-dependent programed cell death in macrophages, thus
hindering an efficient innate antibacterial response. Collectively, our results
highlight the role of an endopeptidase to create a permissive replication niche in
host cells by inducing the repression of autophagy and apoptosis, along with
metabolic reprogramming, and pointing to the role of RipA in disease pathogenesis.
AN - rayyan-553781351
AU - Shariq, M.
AU - Quadir, N.
AU - Sharma, N.
AU - Singh, J.
AU - Sheikh, J. A.
AU - Khubaib, M.
AU - Hasnain, S. E.
AU - Ehtesham, N. Z.
DO - 10.3389/fimmu.2021.636644
KW - Apoptosis
Tuberculosis
PY - 2021
SN - 1664-3224
ST - Mycobacterium tuberculosis RipA Dampens TLR4-Mediated Host Protective
Response Using a Multi-Pronged Approach Involving Autophagy, Apoptosis, Metabolic
Repurposing, and Immune Modulation
T2 - FRONTIERS IN IMMUNOLOGY
TI - Mycobacterium tuberculosis RipA Dampens TLR4-Mediated Host Protective
Response Using a Multi-Pronged Approach Involving Autophagy, Apoptosis, Metabolic
Repurposing, and Immune Modulation
VL - 12
Y2 - 3 y3 - 4
ID - 9293
ER -
TY - JOUR
AB - Airborne particulate matter (PM) was sampled by use of an electrostatic
sampler in an oven hall and a receiving hall in a waste-incineration energy plant,
and from urban air in a heavy-traffic street and from background air in Copenhagen.
PM was sampled for 1-2 weeks, four samples at each site. The samples were extracted
and examined for mutagenicity in Salmonella typhimurium strains TA98, YG1041 and
YG5161, for content of inorganic elements and for the presence of eight polycyclic
aromatic hydrocarbons. The induction of IL-6 and IL-8 mRNA expression and the
presence of DNA damage - tested by the comet assay - were determined after 24-h
incubations with human A549 lung epithelial cells. The PM2.5 concentration was
about twofold greater in the oven hall than in the receiving hall. The particle
size distribution in the receiving hall was similar to that in street air (maximum
mode at about 25 nm), but the distribution was completely different in the oven
hall (maximum mode at about 150 nm). Also chemically, the samples from the oven
hall were highly different from the other samples. PM extracts from the receiving
hall, street and background air were more mutagenic than the PM extracts from the
oven hall. PM from all four sites caused similar levels of DNA damage in A549
cells; only the oven hall samples gave results that were statistically
significantly different from those obtained with street-air samples. The receiving
hall and the urban air samples were similarly inflammatory (relative IL-8 mRNA
expression), whereas the oven hall did not cause a statistically significant
increase in IL-8 mRNA expression. A principal component analysis separated the oven
hall and the receiving hall by the first principal component. These two sites were
separated from street and background air with the second principal component.
Several clusters of constituents were identified. One cluster consisted of all the
polycyclic aromatic hydrocarbons (PAH), several groups of metals and one group of
the biological endpoints (DNA damage, IL-6 and IL-8 mRNA expression). The PAH and
the inorganic content of the air in the receiving hall may be due to vehicle
emissions and suspended waste particles. The inorganic content in the street and
background air may have been influenced by break wear, road emissions and long-
range transport. The results from a partial least-square regression analysis
predicted that both PAHs and a group of metals including Fe and Mn contributed to
IL-6 and IL-8 induction. Only Mn and Sr were predicted to influence DNA damage
statistically significantly. Among the PAHs only chrysene had influence on DNA
damage. The PM from the oven hall was markedly different from the PM at other
locations in particle size distribution, chemical composition and the resulting
biological effects when A549 cells were incubated with the PM. These
characteristics and observations in the oven hall indicated that the PM source was
oven exhaust, which was well combusted. © 2007 Elsevier B.V. All rights reserved.
AN - rayyan-553781352
AU - Sharma, A. K.
AU - Jensen, K. A.
AU - Rank, J.
AU - White, P. A.
AU - Lundstedt, S.
AU - Gagne, R.
AU - Kristiansen, J.
AU - Vogel, U.
AU - Wallin, H.
DO - 10.1016/j.mrgentox.2007.05.013
IS - 2
KW - Chemical composition
Genotoxicity
Incineration energy plant
Inflammation
Particle size distribution
Urban air
Air Pollutants
Comet Assay
Humans
Incineration
Inorganic Chemicals
Metals
Mutagenicity Tests
Particle Size
Particulate Matter
Polycyclic Hydrocarbons, Aromatic
Salmonella typhimurium
aluminum
barium
boron
cadmium
calcium
chromium
copper
DNA
inorganic compound
interleukin 6
interleukin 8
iron
lead
magnesium
manganese
messenger RNA
molybdenum
nickel
phosphorus
potassium
silicon
silver
sodium
strontium
tin
titanium
vanadium
zinc
zirconium
air sampling
airborne particle
article
comet assay
controlled study
cytotoxicity
DNA damage
epithelium cell
frameshift mutation
genotoxicity
human
human cell
incineration
inflammation
lung alveolus epithelium
mutagenicity
partial least squares regression
particle size
principal component analysis
priority journal
Salmonella
statistical significance
PY - 2007
SP - 95-111
ST - Genotoxicity, inflammation and physico-chemical properties of fine particle
samples from an incineration energy plant and urban air
T2 - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
TI - Genotoxicity, inflammation and physico-chemical properties of fine particle
samples from an incineration energy plant and urban air
34547828295&doi=10.1016%2fj.mrgentox.2007.05.013&partnerID=40&md5=3eac935774ba4fa87
19b700dbfc7a368
VL - 633
ID - 9294
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have inconsistent findings against inflammation.
Although a wealth of literature on the beneficial effects of green-synthesized
AgNPs has been published, a detailed mechanistic study of green AgNPs on the
protective effects against lipopolysaccharide (LPS)-induced neuroinflammation using
human microglial cells (HMC3) has not yet been reported. For the first time, we
studied the inhibitory effect of biogenic AgNPs on inflammation and oxidative
stress induced by LPS in HMC3 cells. X-ray photoelectron spectroscopy, Fourier-
transform infrared spectroscopy, and transmission electron microscopy were used to
characterize AgNPs produced from honeyberry. Co-treatment with AgNPs significantly
reduced mRNA expressions of inflammatory molecules such as interleukin (IL)-6 and
tumor necrosis factor-α, while increasing the expressions of anti-inflammatory
markers such as IL-10 and transforming growth factor (TGF)-β. HMC3 cells were also
switched from M1 to M2, as shown by lower expression of M1 markers such as cluster
of differentiation (CD)80, CD86, and CD68 and higher expression of M2 markers such
as CD206, CD163, and triggering receptors expressed on myeloid cells (TREM2).
Furthermore, AgNPs inhibited LPS-induced toll-like receptor (TLR)4 signaling, as
evidenced by decreased expression of myeloid differentiation factor 88 (MyD88) and
TLR4. In addition, AgNPs reduced the production of reactive oxygen species (ROS)
and enhanced the expression of nuclear factor-E2-related factor 2 (Nrf2) and heme
oxygenase-1 (HO-1), while decreasing the expression of inducible nitric oxide
synthase. The docking score of the honeyberry phytoconstituents ranged from -14.93
to - 4.28 KJ/mol. In conclusion, biogenic AgNPs protect against neuroinflammation
and oxidative stress by targeting TLR4/MyD88 and Nrf2/HO-1 signaling pathways in a
LPS-induced in vitro model. Biogenic AgNPs could be utilized as potential
nanomedicine against LPS-induced inflammatory disorders.
AN - rayyan-553782013
AU - Sharma, A.
AU - Sanjay
AU - Jaiswal, V.
AU - Park, M.
AU - Lee, H. J.
DO - 10.1016/j.bioadv.2023.213363
J2 - Biomater Adv
KW - Humans
*Lipopolysaccharides/toxicity
Myeloid Differentiation Factor 88/genetics/metabolism/pharmacology
Molecular Docking Simulation
Silver/pharmacology
NF-E2-Related Factor 2/genetics/metabolism/pharmacology
Neuroinflammatory Diseases
Heme Oxygenase-1/genetics/metabolism/pharmacology
Toll-Like Receptor 4/genetics/metabolism
*Metal Nanoparticles/therapeutic use
Signal Transduction
Phenotype
Cell Line
Inflammation/chemically induced/metabolism
Brain/metabolism
Humanities
Humanism
Brain
LA - eng
N1 - College of BioNano Technology, Department of Food and Nutrition, Gachon
University, Gyeonggi-do 13120, Republic of Korea.; Department of Food Science and
Biotechnology, Gachon University, Gyeonggi-do 13120, Republic of Korea.; College of
BioNano Technology, Department of Food and Nutrition, Gachon University, Gyeonggi-
do 13120, Republic of Korea.; College of BioNano Technology, Department of Food and
Nutrition, Gachon University, Gyeonggi-do 13120, Republic of Korea; Institute for
Aging and Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120,
Republic of Korea.; College of BioNano Technology, Department of Food and
Nutrition, Gachon University, Gyeonggi-do 13120, Republic of Korea; Institute for
Aging and Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120,
Republic of Korea; Department of Health Sciences and Technology, GAIHST, Gachon
University, Incheon 21999, Republic of Korea. Electronic address:
skysea@gachon.ac.kr.
PY - 2023
SP - 213363
ST - Biogenic silver NPs alleviate LPS-induced neuroinflammation in a human fetal
brain-derived cell line: Molecular switch to the M2 phenotype, modulation of
TLR4/MyD88 and Nrf2/HO-1 signaling pathways, and molecular docking analysis
T2 - Biomaterials advances
TI - Biogenic silver NPs alleviate LPS-induced neuroinflammation in a human fetal
brain-derived cell line: Molecular switch to the M2 phenotype, modulation of
TLR4/MyD88 and Nrf2/HO-1 signaling pathways, and molecular docking analysis
VL - 148
Y2 - 5
ID - 9929
ER -
TY - JOUR
AB - The biogenic tailoring of silver nanoparticles using plant extract is
becoming an attractive approach in the current scenario. Manilkara zapota (MZ) is
well known for its antibacterial, hepato-protective, anti-inflammatory, anti-
tussive, anti-fungal, anti-tumour, and free radical scavenging potential. Its
plants extract is a rich source of secondary metabolites. Nowadays, silver
nanoparticles (AgNPs) have been advocated for a variety of biomedical applications.
In present work, silver nanoparticles have been synthesized using an aqueous
extract of MZ, physicochemically characterized and finally evaluated for
antimicrobial effects, catalytic reduction/degradation of organic dyes and
cytotoxicity. The nanosized AgNPs (~ 84 nm) were found to possess prominent
antibacterial potential against gram positive and gram negative pathogens (MIC
50 μg/ml) in comparison to native plant extract. Moreover, these particles were
found to be non-toxic and efficient eradicators of environmental toxicants via
rapid catalytic reduction of toxic chemicals and dyes. Altogether, these results
suggest promising potential of these nanoparticles that can be used as
multifunctional agents for future biomedical applications. © 2020, Association of
Microbiologists of India.
AN - rayyan-553781353
AU - Sharma, B.
AU - Singh, I.
AU - Bajar, S.
AU - Gupta, S.
AU - Gautam, H.
AU - Kumar, P.
DO - 10.1007/s12088-020-00889-0
IS - 4
KW - Antibacterial
Antibiofilm
Antioxidant activity
Catalytic reduction
Manilkara zapota
Phytonanotechnology
silver nanoparticle
animal cell
antifungal activity
antitussive activity
Article
bioassay
biogenesis
biological activity
catalysis
cellular parameters
controlled study
cytocompatibility
cytotoxicity
disk diffusion
drug degradation
drug synthesis
human
human cell
microtitre plate assay
nonhuman
physical chemistry
Raman spectrometry
X ray diffraction
zone of inhibition
PY - 2020
SP - 468-474
ST - Biogenic Silver Nanoparticles: Evaluation of Their Biological and Catalytic
Potential
T2 - Indian Journal of Microbiology
TI - Biogenic Silver Nanoparticles: Evaluation of Their Biological and Catalytic
Potential
85085361331&doi=10.1007%2fs12088-020-00889-
0&partnerID=40&md5=d2bf4abeefcf3a3de816b1fbe620fdef
VL - 60
ID - 9295
ER -
TY - JOUR
AB - We synthesized and characterized curcumin-stabilized silver nanoparticles
(Cur-AgNP) and found them to be 45 nm by dynamic light scattering with a maximum
absorbance at 406 nm. We evaluated Cur-AgNP for immunomodulatory activities and
their potential as an antiretroviral agent. The antiretroviral effects of Cur-AgNP
were determined in ACH-2 cells latently infected with human immunodeficiency virus
(HIV)-1. ACH-2 cells, 200,000/ml, were treated with Cur-AgNP for 24-48 h.
Expression of HIV-1 LTR and p24, the pro-inflammatory cytokines, IL-1β, TNF-α, and
NF-κB was quantitated. Treatment of ACH-2 cells latently infected with HIV-1 with
Cur-AgNP produced no toxic effects but significantly inhibited the expression of
HIV-1 LTR (-73%, P < 0.01) and p24 (-57%, P < 0.05), IL-1βα (-61%, P < 0.01), TNF-
αα (-54%, P < 0.05), IL-6 (-68%, P < 0.01), and NF-κB (-79%, P < 0.0001) as
compared to untreated controls. Thus, Cur-AgNP have therapeutic potential as direct
antiretroviral agents, as well as having immunomodulatory activities inhibiting the
expression of pro-inflammatory mediators induced by infection with HIV-1.
Experimental controls, such as curcumin alone, and conventional silver
nanoparticles capped with citric acid, produced no similar biological effects. We
conclude that treatment of HIV-1 infected cells with Cur-AgNP significantly reduced
replication of HIV by inhibition of NF-κB nuclear translocation and the downstream
expression of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. Subsequent in
vivo studies with Cur-AgNP using a humanized mouse model of HIV infection are
underway.
AN - rayyan-553781944
AU - Sharma, R. K.
AU - Cwiklinski, K.
AU - Aalinkeel, R.
AU - Reynolds, J. L.
AU - Sykes, D. E.
AU - Quaye, E.
AU - Oh, J.
AU - Mahajan, S. D.
AU - Schwartz, S. A.
DO - 10.1080/08820139.2017.1371908
IS - 8
J2 - Immunol Invest
KW - Anti-Retroviral Agents/*pharmacology
Cell Line
Curcumin/chemistry/*pharmacology
HIV Core Protein p24/metabolism
HIV Infections/*immunology
HIV Long Terminal Repeat/genetics
HIV-1/*physiology
Humans
Immunologic Factors/*pharmacology
Metal Nanoparticles/chemistry/*therapeutic use
Silver/chemistry
T-Lymphocytes/*immunology/pathology/virology
Virus Latency
Virus Replication
LA - eng
N1 - a Department of Medicine Division of Allergy, Immunology, and Rheumatology,
University at Buffalo , Clinical and Translational Research Center , NY , USA.; a
Department of Medicine Division of Allergy, Immunology, and Rheumatology,
University at Buffalo , Clinical and Translational Research Center , NY , USA.
PY - 2017
SP - 833-846
ST - Immunomodulatory activities of curcumin-stabilized silver nanoparticles:
Efficacy as an antiretroviral therapeutic
T2 - Immunological investigations
TI - Immunomodulatory activities of curcumin-stabilized silver nanoparticles:
Efficacy as an antiretroviral therapeutic
VL - 46
Y2 - 11
ID - 9864
ER -
TY - CHAP
AB - With the growing use of nanomaterials in bioapplications, the nanotoxicity of
new nanomaterials has become a safety concern when used in various applications. In
this chapter, technical developments on carbon nanotubes are described including a
historical account, experimental models and potential bioapplications. Carbon
nanotube (CNT) materials display superior properties in electric current carrying
capacity, thermal conductivity, and thermal stability. Due to the unique CNT
structure with high-aspect ratio, CNT may show unusual toxicity and complicate its
safe use in a target tissue. To test nanotoxicity of CNT, we describe a set of
protocols of prior knowledgebased physical and chemical characteristics to develop
3-dimensional in vitro models of the intact skin, and a 3D in vitro model of the
human airway using a co-culture of normal human bronchial epithelial cells and
normal human fibroblasts. The human airway 3D model served as a tool of health risk
assessment of CNTs on the human respiratory systems. To test functionality at
different CNT concentrations in a 3D model, physical characteristics of multiwalled
CNTs and production of nitric oxide (NO) served as cell viability and inflammatory
marker; mitochondrial activity (MTT assay) served as the cytotoxic response of the
epithelial cell layers; transepithelial electrical resistance (TER) measured
nanotoxicity in the changes in airway physiological function. Cytoxicity and
inflammatory responses of CNTs were dependent on different size, mass, shape, and
functionality of CNTs as viable in vivo tests were conducted to evaluate the
toxicity of engineered CNTs. We monitored the transport across skin, and the
physiological perturbation of transepithelial electrical resistance (TER) during
the exposure of different concentrations of CNTs. The mechanisms of CNTs' toxicity
are closely related to their structure, functional group, and surface charge on the
molecule. We established the nanoscale toxicity of fullerenes of CNTs. © 2013 by
Scrivener Publishing LLC. All rights reserved.
AN - rayyan-553781354
AU - Sharma, R.
AU - Kwon, S.
DO - 10.1002/9781118644591.ch3
KW - Carbon nanotube
Cytotoxicity
Drug carrier
Fullerene
Nanotoxicity
Nanotubes, Carbon
PY - 2013
SP - 99-144
ST - Carbon Nanotubes: Nanotoxicity Testing and Bioapplications
T2 - Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering
TI - Carbon Nanotubes: Nanotoxicity Testing and Bioapplications
84887144826&doi=10.1002%2f9781118644591.ch3&partnerID=40&md5=1331ee2a08fedbb66984c4
c110669575
ID - 9296
ER -
TY - JOUR
AB - Wound dressings have become a crucial treatment for wound healing due to
their convenience, low cost, and prolonged wound management. As cutting-edge
biomaterials, marine polysaccharides are divided from most marine organisms. It
possesses various bioactivities, which al-lowing them to be processed into various
forms of wound dressings. Therefore, a comprehensive understanding of the
application of marine polysaccharides in wound dressings is particularly important
for the studies of wound therapy. In this review, we first introduce the wound
healing process and describe the characteristics of modern commonly used dressings.
Then, the properties of various marine polysaccharides and their application in
wound dressing development are outlined. Finally, strategies for developing and
enhancing marine polysaccharide wound dressings are de-scribed, and an outlook of
these dressings is given. The diverse bioactivities of marine polysaccharides
including antibacterial, anti-inflammatory, haemostatic properties, etc., providing
excellent wound management and accelerate wound healing. Meanwhile, these
biomaterials have higher biocompatibility and biodegradability compared to
synthetic ones. On the other hand, marine polysaccharides can be combined with
copolymers and active substances to prepare various forms of dressings. Among them,
emerging types of dressings such as nanofibers, smart hydrogels and injectable
hydrogels are at the research frontier of their development. Therefore, marine
polysaccharides are essential materials in wound dressings fabrication and have a
promising future. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553781358
AU - Shen, S.
AU - Chen, X.
AU - Shen, Z.
AU - Chen, H.
IS - 10
KW - Agar
Alginate
Biomaterials
Biopolymers
Carrageenan
Chitosan
Fu-coidan
Ulvan
Wound dressing
Wound healing
5 hydroxymethylfurfural
agar
agarose
alginic acid
andrographolide
Arnica montana extract
bioceramics
biomaterial
borate sodium
calcium sulfate
Calendula extract
carbon nanotube
carrageenan
chitin
chitosan
chondroitin sulfate
ciprofloxacin
copolymer
curcumin
dermatan sulfate
dopamine
doxorubicin
doxycycline
erythromycin
essential oil
exopolysaccharide
Forsythia extract
fucoidin
gel
genipin
gentamicin
glass
glycosaminoglycan
graphene
graphene oxide
heparan sulfate
heparin
hyaluronic acid
hydrogel
ibuprofen
keratan sulfate
laminaran
lidocaine
macrogol
marine polysaccharide
melatonin
minocycline
mupirocin
nanofiber
naproxen
polycaprolactone
polylactic acid
polysaccharide
Schiff base
silver
silver nanoparticle
stearic acid
streptomycin
tannin
tetracycline
thrombocyte concentrate
tranexamic acid
ulvan
unclassified drug
vancomycin
vasculotropin
zinc ion
zinc nanoparticle
zinc oxide
Aloe vera
biocompatibility
biodegradability
biomechanics
chemical structure
coating (procedure)
drug release
emulsion
gelation
hemostasis
honey
human
nanofabrication
nonhuman
oregano
Review
thermostability
tissue regeneration
wound care
wound healing
Polysaccharides
Military Personnel
Bandages
PY - 2021
ST - Marine polysaccharides for wound dressings application: An overview
T2 - Pharmaceutics
TI - Marine polysaccharides for wound dressings application: An overview
85117787885&doi=10.3390%2fpharmaceutics13101666&partnerID=40&md5=104017426870c98535
8d90f8e07b2caa
VL - 13
ID - 9300
ER -
TY - JOUR
AB - Presbycusis contributes to cognitive decline and Alzheimer's disease.
However, most research in this area involves clinical observations and statistical
modeling, and few studies have examined the relationship between hearing loss and
the molecular changes that lead to cognitive dysfunction. The present study
investigated whether hearing loss contributes to dementia in the absence of aging
and noise using a mouse model of severe bilateral hearing loss induced by kanamycin
(1000 mg/kg) and furosemide (400 mg/kg). Immunohistochemistry, silver staining,
immunofluorescence analysis, and Western blotting were used to observe pathological
changes in different regions of the hippocampus in animals with hearing loss.
Changes in the cognitive function of animals with hearing loss were assessed using
the Morris water maze test. The results showed that neurons began to degenerate
60 days after hearing loss, and this degeneration was accompanied by structural
disorganization and decreased neurogenesis. The level of phosphorylated tau
increased over time. Increases in escape latency and distance traveled during the
training phase of the Morris water maze test were observed 90 days after hearing
loss. Activated microglia and astrocytes with increased levels of inflammatory
cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were
detected in the hippocampus. These results suggest that hearing loss alone causes
neuronal degeneration, inhibition of neurogenesis, increased tau protein
phosphorylation, and increased neuroinflammation in the hippocampus. Early
intervention in individuals with hearing loss may reduce the risk of cognitive
decline.
AN - rayyan-553782347
AU - Shen, Y.
AU - Hu, H.
AU - Fan, C.
AU - Wang, Q.
AU - Zou, T.
AU - Ye, B.
AU - Xiang, M.
DO - 10.1016/j.nbd.2021.105408
J2 - Neurobiol Dis
KW - Animals
Dementia/chemically induced/metabolism/*pathology
Female
Furosemide/toxicity
Hearing Loss, Sensorineural/chemically induced/metabolism/*pathology
Hippocampus/drug effects/metabolism/*pathology
Kanamycin/toxicity
Male
Maze Learning/drug effects/physiology
Mice
Mice, Inbred C57BL
Neurons/drug effects/metabolism/*pathology
tau Proteins/metabolism
Dementia
LA - eng
N1 - Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital,
Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key
Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China.;
Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai
Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory
of Translational Medicine on Ear and Nose Diseases, Shanghai, China.; Department of
Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai Jiao Tong
University School of Medicine, Shanghai, China; Shanghai Key Laboratory of
Translational Medicine on Ear and Nose Diseases, Shanghai, China.; Department of
Translational Medicine on Ear and Nose Diseases, Shanghai, China. Electronic
address: aydyebin@126.com.; Department of Otolaryngology & Head and Neck Surgery,
Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China;
Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases,
Shanghai, China. Electronic address: mingliangxiang@163.com.
PY - 2021
SP - 105408
ST - Sensorineural hearing loss may lead to dementia-related pathological changes
in hippocampal neurons
T2 - Neurobiology of disease
TI - Sensorineural hearing loss may lead to dementia-related pathological changes
in hippocampal neurons
VL - 156
Y2 - 8
ID - 10255
ER -
TY - JOUR
AB - Background: Nanopopcorns are a novel class of metallic nanoparticles that
demonstrate structural similarity to the grains of popcorns with theranostic
activities for diseases like cancer and bacterial infection using Surface Enhanced
Raman Spectroscopy-based detection. Objective: The objective of the present article
is to highlight the importance of popcorn-shaped nanoparticles for the treatment of
various disease conditions like cancer, diabetes, ulcerative colitis, rheumatoid
arthritis, etc. Methods: Nanopopcorns enter the target cells via conjugation with
various proteins, aptamers, etc. to kill the diseased cell. Moreover, external
magnetic radiations are provided to heat these metallic nanopopcorns for creating
hotspots. All such activities can be tracked via SERS mechanism. Results:
Nanopopcorns create alternative and minimally-invasive treatment strategies for
inflammatory conditions and life-threatening diseases. Conclusion: In the near
future, nanopopcorn-based drug delivery system can be an interesting field for
research in medicinal nanotechnology. © 2021 Bentham Science Publishers.
AN - rayyan-553781359
AU - Shende, P.
AU - Deshpande, G.
DO - 10.2174/1573413716999201209105519
IS - 5
KW - Apoptosis
Cell imaging
Hotspots
Hyperthermia
Metallic nanoparticles
Surface enhanced raman spectroscopy
Theranostics
Cell death
Drug delivery
Light transmission
Metals
Nanoparticles
Raman spectroscopy
dye
gold nanoparticle
metal nanoparticle
nanomaterial
nanorod
nanosphere
pesticide
protective agent
silver nanoparticle
Metallics
Multi-modal approach
Structural similarity
Surface enhanced Raman spectroscopy
apoptosis
Article
bacterium
chemical interaction
conjugation
diabetes mellitus
electromagnetism
human
magnetic field
malignant neoplasm
particle size
theranostic nanomedicine
thermogravimetry
ulcerative colitis
zeta potential
Diseases
PY - 2021
SP - 670-678
ST - Metallic nanopopcorns: A new multimodal approach for theranostics
T2 - Current Nanoscience
TI - Metallic nanopopcorns: A new multimodal approach for theranostics
85119695856&doi=10.2174%2f1573413716999201209105519&partnerID=40&md5=24083bb8b6790f
7a082dabcc40888564
VL - 17
ID - 9301
ER -
TY - JOUR
AB - Objectives. Silver has long been known as a strong antimicrobial and
disinfectant. Several types of nano-silver coated products have been developed.
However, the antimicrobial and disinfectant characteristics of nano-silver have not
been well studied. The aim of this study was to investigate the effect of nano-
silver on allergic inflammation in a mouse model. Methods. Female BALB/C mice were
sensitized by intraperitoneal injection of ovalbumin (OVA) and aluminium hydroxide
on days 0, 7, 14, and 21. Mice were challenged with intranasal instillation of OVA.
Nano-silver was also administered nasally prior to intranasal instillation of OVA.
Severity of allergic rhinitis was assessed according to nasal symptoms, serum OVA-
specific IgE level, interleukin (IL)-4, IL-10, and interferon (INF)-gamma levels in
nasal lavage fluid. Hematoxylin-eosin stain and periodic acid-Schiff stain were
performed for evaluation of histological change. Results. Nano-silver attenuated
manifestation of nasal symptoms in sensitized mice and inhibited production of OVA-
specific IgE, IL-4, and IL-10, however, it had no effect on INF-gamma level. In
addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia
was attenuated by nano-silver. Conclusion. These results suggest that nano-silver
may effectively reduce allergic inflammation in a mouse model of allergic
rhinitis.Through its properties as an anti-inflammatory agent, nano-silver may be a
useful therapeutic strategy.
AN - rayyan-553781364
AU - Shin, S. H.
AU - Ye, M. K.
DO - 10.3342/ceo.2012.5.4.222
IS - 4
KW - Mice
PY - 2012
SN - 1976-8710
SP - 222-227
ST - The Effect of Nano-Silver on Allergic Rhinitis Model in Mice
T2 - CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
TI - The Effect of Nano-Silver on Allergic Rhinitis Model in Mice
VL - 5
Y2 - 12
ID - 9306
ER -
TY - JOUR
AB - The most frequent complication in external fixation is pin tract infection.
To reduce the incidence of implant-associated infection, many published reports
have looked at preventing bacterial adhesion by treating the pin surface. This
study aimed to evaluate the antibacterial activity of a Titanium-Copper (Ti-Cu)
alloy on implant infection, and to determine the potential use of the Ti-Cu alloy
as a biomaterial. Two forms of Ti-Cu alloys were synthesized: one with 1% Cu and
the other with 5% Cu. For analyzing infectious behavior, the implants were exposed
to Staphylococcus aureus and Escherichia coli. The reaction of pathogens to the Ti-
Cu alloys was compared with their reaction to stainless steel and pure titanium as
controls. Both Ti-Cu alloys evidently inhibited colonization by both bacteria.
Conversely, cytocompatibility studies were performed using fibroblasts and colony
formation on the metals was assessed by counting the number of colonies. Ti-1% Cu
alloy showed no difference in the number of colonies compared with the control.
External fixator pins made of Ti-Cu alloys were evaluated in a rabbit model. The
tissue-implant interactions were analyzed for the presence of infection,
inflammatory changes and osteoid-formation. Ti-1% Cu alloy significantly inhibited
inflammation and infection, and had excellent osteoid-formation. Copper blood
levels were measured before surgery and at 14 days postoperatively. Preoperative
and postoperative blood copper values were not statistically different. Overall, it
was concluded that Ti-Cu alloys have antimicrobial activity and substantially
reduce the incidence of pin tract infection. Ti-1% Cu alloy shows particular
promise as a biomaterial. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part
B: Appl Biomater 91B: 373-380, 2009
AN - rayyan-553781366
AU - Shirai, T.
AU - Tsuchiya, H.
AU - Shimizu, T.
AU - Ohtani, K.
AU - Zen, Y.
AU - Tomita, K.
DO - 10.1002/jbm.b.31412
IS - 1
KW - Titanium
Copper
PY - 2009
SN - 1552-4973 1552-4981
SP - 373-380
ST - Prevention of Pin Tract Infection with Titanium-Copper Alloys
TI - Prevention of Pin Tract Infection with Titanium-Copper Alloys
VL - 91
Y2 - 10
ID - 9308
ER -
TY - JOUR
AB - EMBL-EBI The European Bioinformatics Institute; E. coli Escherichia coli; E.
faecalis Enterobacter faecalis; B. fragilis Bacteroides fragilis; B. vulgatus
Bacteroides vulgatus; SaPIs Staphylococcus aureus pathogenicity islands; ARGs
Antibiotic resistance genes; STEC Shiga toxigenic E. coli; Stx Shiga toxin; BLAST
Basic Local Alignment Search Tool; TSST-1 Toxic shock toxin 1; RBPs Receptor-
binding proteins; LPS lipopolysaccharide; OMVs Outer membrane vesicles; PT
Phosphorothioate; BREX Bacteriophage exclusion; OCR Overcome classical restriction;
Pgl Phage growth limitation; DISARM Defense island system associated with
restrictionmodification; R-M system Restriction-modification system; BREX system
Bacteriophage exclusion system; CRISPR Clustered regularly interspaced short
palindromic repeats; Cas CRISPR-associated; PAMs Prospacer adjacent motifs; crRNA
CRISPR RNA; SIE; OMPs; Superinfection exclusion; Outer membrane proteins; Abi
Abortive infection; TA Toxin-antitoxin; TLR Toll-like receptor; APCs Antigen-
presenting cells; DSS Dextran sulfate sodium; IELs Intraepithelial lymphocytes; FMT
Fecal microbiota transfer; IFN-γ Interferon-gamma; IBD Inflammatory bowel disease;
AgNPs Silver nanoparticles; MDSC Myeloid-derived suppressor cell; CRC Colorectal
cancer; VLPs Virus-like particles; TMP Tape measure protein; PSMB4 Proteasome
subunit beta type-4; ALD Alcohol-related liver disease; GVHD Graft-versus-host
disease; ROS Reactive oxygen species; RA Rheumatoid arthritis; CCP Cyclic
citrullinated protein; AMGs Accessory metabolic genes; T1DM Type 1 diabetes
mellitus; T2DM Type 2 diabetes mellitus; SCFAs Short-chain fatty acids; GLP-1
Glucagon-like peptide-1; A. baumannii Acinetobacter baumannii; CpG
Deoxycytidylinate-phosphodeoxyguanosine; PEG Polyethylene glycol; MetS Metabolic
syndrome; OprM Outer membrane porin M.
AN - rayyan-553782341
AU - Shuwen, H.
AU - Kefeng, D.
DO - 10.1080/19490976.2022.2113717
IS - 1
J2 - Gut Microbes
KW - Bacteria
*Bacteriophages/genetics
*Diabetes Mellitus, Type 2
Escherichia coli
*Gastrointestinal Microbiome
Humans
Proteasome Endopeptidase Complex/metabolism
Silver/metabolism
Humanities
Humanism
Intestines
LA - eng
N1 - Department of Colorectal Surgery and Oncology, Key Laboratory of Cancer
Prevention and Intervention, Ministry of Education, The Second Affiliated Hospital,
Zhejiang University School of Medicine, Hangzhou, China.; Department of Medical
Oncology, Huzhou Central Hospital, Huzhou, China.; Department of Colorectal Surgery
and Oncology, Key Laboratory of Cancer Prevention and Intervention, Ministry of
Education, The Second Affiliated Hospital, Zhejiang University School of Medicine,
Hangzhou, China.; Department of Colorectal Surgery and Oncology, Cancer Center
Zhejiang University, Hangzhou, China.
PY - 2022
SP - 2113717
ST - Intestinal phages interact with bacteria and are involved in human diseases
T2 - Gut microbes
TI - Intestinal phages interact with bacteria and are involved in human diseases
VL - 14
Y2 - 1
ID - 10249
ER -
TY - JOUR
AB - BACKGROUND: Wounds cause structural and functional discontinuity of an organ.
Wound healing, therefore, seeks to re-establish the normal morphology and
functionality through intertwined stages of hemostasis, inflammation,
proliferation, and tissue remodelling. Ivermectin, a macrolide, has been used as an
endectoparasiticide in human and veterinary medicine practice for decades. Here, we
show that ivermectin exhibits wounding healing activity by mechanisms independent
of its well-known antiparasitic activity. This study aimed to evaluate the wound
healing property of ivermectin cream using histochemistry and enzyme-linked
immunosorbent assay techniques. RESULTS: Non-irritant dose of ivermectin cream
(0.03-1%) decreased wound macroscopic indices such as exudation, edge edema,
hyperemia, and granulation tissue deposition by day 9 compared to day 13 for the
vehicle-treated group. This corresponded with a statistically significant wound
contraction rate, hydroxyproline deposition, and a decreased time to heal rate. The
levels of growth factors TGF-β1 and VEGF were significantly elevated on day 7 but
decreased on day 21. This corresponded with changes in cytokines (IL-1α, IL-4, IL-
10, and TNF-α) and eicosanoids (LTB4, PGE(2), and PGD(2)) levels on days 7 and 21.
(.) Interestingly, low doses of ivermectin cream (0.03-0.1%) induced wound healing
with minimal scarring compared to higher doses of the cream and the positive
control, Silver Sulfadiazine. CONCLUSION: Ivermectin promotes wound healing partly
through modulation of the inflammatory process and the levels of Transforming
Growth Factor-Beta 1 and Vascular Endothelial Growth Factor. Low doses of
ivermectin cream have the potential to be used in treating wounds with minimal scar
tissue formation.
AN - rayyan-553782366
AU - Sia, D. K.
AU - Mensah, K. B.
AU - Opoku-Agyemang, T.
AU - Folitse, R. D.
AU - Darko, D. O.
DO - 10.1186/s12917-020-02612-z
IS - 1
J2 - BMC Vet Res
KW - Animals
Ivermectin/*pharmacology/toxicity
Male
Silver Sulfadiazine/pharmacology
Skin/*drug effects
Transforming Growth Factor beta1/metabolism
Wound Healing
LA - eng
N1 - Department of Pharmacology, College of Health Sciences, Kwame Nkrumah
University of Science and Technology, Kumasi, Ghana.; School of Veterinary
Medicine, College of Health Sciences, Kwame Nkrumah University of Science and
Technology, Kumasi, Ghana.; Department of Pharmacology, College of Health Sciences,
Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
kbmensah.pharm@knust.edu.gh.; School of Veterinary Medicine, College of Health
Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.;
School of Veterinary Medicine, College of Health Sciences, Kwame Nkrumah University
of Science and Technology, Kumasi, Ghana.; Department of Pharmacology, College of
Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
PY - 2020
SP - 397
ST - Mechanisms of ivermectin-induced wound healing
T2 - BMC veterinary research
TI - Mechanisms of ivermectin-induced wound healing
VL - 16
Y2 - 10 y3 - 20
ID - 10273
ER -
TY - JOUR
AB - Mercury is one of the most toxic elements and causes a multitude of health
problems. It is ten times more toxic to neurons than lead. This study was created
to determine if mercury could be causing Alzheimer's disease (AD) by cross
referencing the effects of mercury with 70 factors associated with AD. The results
found that all these factors could be attributed to mercury. The hallmark changes
in AD include plaques, beta amyloid protein, neurofibrillary tangles,
phosphorylated tau protein, and memory loss-all changes that can be caused by
mercury. Neurotransmitters such as acetylcholine, serotonin, dopamine, glutamate,
and norepinephrine are inhibited in patients with Alzheimer's disease, with the
same inhibition occurring in mercury toxicity. Enzyme dysfunction in patients with
Alzheimer's disease include BACE 1, gamma secretase, cyclooxygenase-2, cytochrome-
c-oxidase, protein kinases, monoamine oxidase, nitric oxide synthetase, acetyl
choline transferase, and caspases, all which can be explained by mercury toxicity.
Immune and inflammatory responses seen in patients with Alzheimer's disease also
occur when cells are exposed to mercury, including complement activation, cytokine
expression, production of glial fibrillary acid protein antibodies and interleukin-
1, transforming growth factor, beta 2 microglobulins, and phosphodiesterase 4
stimulation. Genetic factors in patients with Alzheimer's disease are also
associated with mercury. Apolipoprotein E 4 allele increases the toxicity of
mercury. Mercury can inhibit DNA synthesis in the hippocampus, and has been
associated with genetic mutations of presenilin 1 and 2, found in AD. The
abnormalities of minerals and vitamins, specifically aluminum, calcium, copper,
iron, magnesium, selenium, zinc, and vitamins B1, B12, E, and C, that occur in
patients with Alzheimer's disease, also occur in mercury toxicity. Aluminum has
been found to increase mercury's toxicity. Likewise, similar biochemical factors in
AD are affected by mercury, including changes in blood levels of homocysteine,
arachidonic acid, DHEA sulfate, glutathione, hydrogen peroxide, glycosamine
glycans, acetyl-L carnitine, melatonin, and HDL. Other factors seen in Alzheimer's
disease, such as increased platelet activation, poor odor identification,
hypertension, depression, increased incidences of herpes virus and chlamydia
infections, also occur in mercury exposure. In addition, patients diagnosed with
Alzheimer's disease exhibit higher levels of brain mercury, blood mercury, and
tissue mercury in some studies. The greatest exogenous sources of brain mercury
come from dental amalgams. Conclusion: This review of the literature strongly
suggests that mercury can be a cause of Alzheimer's Disease.
AN - rayyan-553781368
AU - Siblerud, R.
AU - Mutter, J.
AU - Moore, E.
AU - Naumann, J.
AU - Walach, H.
DO - 10.3390/ijerph16245152
IS - 24
KW - Alzheimer Disease
PY - 2019
SN - 1661-7827 1660-4601
ST - A Hypothesis and Evidence That Mercury May be an Etiological Factor in
Alzheimer's Disease
TI - A Hypothesis and Evidence That Mercury May be an Etiological Factor in
Alzheimer's Disease
VL - 16
Y2 - 12 y3 - 2
ID - 9310
ER -
TY - JOUR
AB - Toxic epidermal necrolysis (TEN) is a rare severe reaction of the skin
resulting in full thickness damage to the epidermis. The condition has significant
morbidity as a result of dehydration, protein loss, thermoregulatory difficulties,
and renal, lung, liver and heart failure. The mortality rate approaches 30%, most
commonly from bacterial sepsis. Management of this condition is cessation of the
suspected causative agent and supportive care on a burns or intensive care unit.
There have been recent reports of treatment using intravenous immunoglobulin (IVIG)
therapy, though its efficacy is yet to be established. It has been proposed that
IVIG inhibits the Fas-FasL mediated apoptosis of keratinocytes affected by TEN. We
describe a case of extensive drug-induced TEN in a 33-year-old woman who showed
rapid improvement with IVIG therapy at a dose of 0.75 g/kg/day given for four
consecutive days.
AN - rayyan-553781371
AU - Sidwell, R. U.
AU - Swift, S.
AU - Yan, C. L.
AU - Porter, W.
AU - Thompson, E. M.
AU - Clark, J. A.
AU - Bunker, C. B.
IS - 7
KW - Adult
Antidepressive Agents, Second-Generation
Antimanic Agents
Carbamazepine
Cyclohexanols
Depression, Postpartum
Epidermal Necrolysis, Toxic
Female
Humans
amphotericin
beclometasone dipropionate
benzalkonium chloride
betamethasone valerate
carbamazepine
clobetasol propionate
dexamethasone
diflucortolone valerate
flucloxacillin
immunoglobulin
myristic acid isopropyl ester
paraffin
penicillin G
sulfadiazine silver
venlafaxine
adult
article
blister
case report
cell death
chemosis
conjunctivitis
cornea erosion
drug induced disease
drug withdrawal
female
general condition deterioration
genital ulcer
human
human cell
human tissue
keratinocyte
mouth ulcer
neutrophilia
priority journal
puerperal depression
rash
skin biopsy
skin defect
skin exfoliation
toxic epidermal necrolysis
treatment outcome
Immunoglobulin D
Immunoglobulin M
Immunoglobulin A
Immunoglobulin E
Immunoglobulins
Stevens-Johnson Syndrome
PY - 2003
SP - 643-645
ST - Treatment of toxic epidermal necrolysis with intravenous immunoglobulin
T2 - International Journal of Clinical Practice
TI - Treatment of toxic epidermal necrolysis with intravenous immunoglobulin
0141502380&partnerID=40&md5=151877d4c5b31131e6feaf9c9618ce46
VL - 57
ID - 9313
ER -
TY - JOUR
AB - Titanium dioxide nanoparticles (TiO2 NP) are present in several daily use
products, and the risks associated with their bioaccumulation must be stablished.
Thus, an evaluation of several toxicological-related effects was conducted after
intraperitoneal injection of TiO2 NPs in mice. Mice were divided into two groups,
which received 2 mg kg(-1) day(-1) of TiO2 NPs or vehicle saline. Assessments of
body and organ weight as well as biochemical, hematological, and histopathological
analyses were performed in order to evaluate adverse effects. The results showed
that treatment resulted in an increased visceral and abdominal fat deposition, as
well as a mononuclear inflammatory infiltrates in the abdominal fat tissue. The
TiO2 NPs induced significant decrease in the weight gain and splenomegaly.
Additionally, TiO2 NP-treated mice showed altered hematological parameters and
significant liver injuries, which were characterized by histopathological and
biochemical changes. Our results also indicated that TiO2 NPs were absorbed and
significantly accumulated in the spleen, liver, and kidney. These results showed
the ability of TiO2 NPs to infiltrate different organs and to induce inflammation
and liver and spleen damage with visceral fat accumulation. The data obtained are
useful for the governmental authorities to legislate and implement regulations
concerning the use and the production of this kind of material that might be
hazardous to the living beings, as well as to the environment.
AN - rayyan-553781373
AU - Silva, A. H.
AU - Locatelli, C.
AU - Filho, U. P. R.
AU - Gomes, B. F.
AU - de Carvalho, R. M.
AU - de Gois, J. S.
AU - Borges, D. L. G.
AU - Creczynski-Pasa, T. B.
DO - 10.1177/0748233715613224
IS - 2
KW - Titanium
Inflammation
Mice
Adipose Tissue
PY - 2017
SN - 0748-2337 1477-0393
SP - 147-158
ST - Visceral fat increase and signals of inflammation in adipose tissue after
administration of titanium dioxide nanoparticles in mice
T2 - TOXICOLOGY AND INDUSTRIAL HEALTH
TI - Visceral fat increase and signals of inflammation in adipose tissue after
administration of titanium dioxide nanoparticles in mice
VL - 33
Y2 - 2
ID - 9315
ER -
TY - JOUR
AB - Naturally-occurring chalcones and synthetic chalcone analogues have been
demonstrated to have many biological effects, including anti-inflammatory, anti-
malarial, anti-fungal, and anti-oxidant/anti-cancerous activities. Compared to
other chalcones, trans-chalcone exhibits superior inhibitory activity in cancer
cell growth as shown via in vitro assays, and exerts anti-cancerous effects via the
activation of the p53 tumor suppressor protein. Thus, characterization of the
specific mechanisms, by which trans-chalcone activates p53, can aid development of
new chemotherapeutic drugs that can be used individually or synergistically with
other drugs. In this report, we found that trans-chalcone modulates many p53 target
genes, HSP40 being the most induced gene in the RNA-Seq data using trans-chalcone-
treated cells. CRM1 is also inhibited by trans-chalcone, resulting in the
accumulation of p53 and other tumor suppressor proteins in the nucleus. Similar
effects were seen using trans-chalcone derivatives. Overall, trans-chalcone could
provide a strong foundation for the development of chalcone-based anti-cancer
drugs.
AN - rayyan-553781374
AU - Silva, G.
AU - Marins, M.
AU - Chaichanasak, N.
AU - Yoon, Y.
AU - Fachin, A. L.
AU - Pinhanelli, V. C.
AU - Regasini, L. O.
AU - dos Santos, M. B.
AU - Ayusso, G. M.
AU - Marques, B. D.
AU - Wu, W. W.
AU - Phue, J. N.
AU - Shen, R. F.
AU - Baek, S. J.
DO - 10.1371/journal.pone.0202263
IS - 8
PY - 2018
SN - 1932-6203
ST - Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1
inhibition
T2 - PLOS ONE
TI - Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1
inhibition
VL - 13
Y2 - 8 y3 - 17
ID - 9316
ER -
TY - JOUR
AB - Zinc oxide NPs (ZnO) have been recently proposed as novel candidates for the
treatment of allergic inflammatory diseases. Paradoxically, recent data suggested
that ZnO could cause eosinophilic airway inflammation in rodents. Despite the above
observations, there are currently no studies reporting direct interaction between a
given NP and human eosinophils themselves. In this study, freshly isolated human
eosinophils were incubated with ZnO and several cellular functions were studied. We
found that ZnO delay human eosinophil apoptosis, partially by inhibiting caspases
and by preventing caspase-4 and Bcl-xL degradation. ZnO do not induce production of
reactive oxygen species but increase de novo protein synthesis. In addition, ZnO
were found to increase the production of the proinflammatory IL-1 beta and IL-8
cytokines. Using a pharmacological approach, we demonstrated that inhibition of
caspase-1 reversed the ability of ZnO to induce IL-1 beta and IL-8 production,
whereas inhibition of caspase-4 only reversed that of IL-8. Our results indicate
the necessity of conducting studies to determine the potential fusing NP as
nanotherapies, particularly in diseases in which eosinophils may be involved. We
conclude that, indeed, human eosinophils represent potential new direct targets to
NPs, ZnO in the present case. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
AN - rayyan-553781375
AU - Silva, L. R.
AU - Girard, D.
DO - 10.1016/j.toxlet.2016.07.020
KW - Humanities
Humanism
Humans
Zinc
Eosinophils
PY - 2016
SN - 0378-4274 1879-3169
SP - 11-20
ST - Human eosinophils are direct targets to nanoparticles: Zinc oxide
nanoparticles (ZnO) delay apoptosis and increase the production of the pro-
inflammatory cytokines IL-1 beta and IL-8
TI - Human eosinophils are direct targets to nanoparticles: Zinc oxide
nanoparticles (ZnO) delay apoptosis and increase the production of the pro-
inflammatory cytokines IL-1 beta and IL-8
VL - 259
Y2 - 9 y3 - 30
ID - 9317
ER -
TY - JOUR
AB - Silver nanoparticle (Ag NP) production methods are being developed and
refined to produce more uniform Ag NPs through chemical reactions involving silver
salt solutions, solvents, and capping agents to control particle formation. These
chemical reactants are often present as contaminants and/or coatings on the Ag NPs,
which could alter their interactions in vivo. To determine pulmonary effects of
citrate-coated Ag NPs, Sprague-Dawley rats were exposed once nose-only to
aerosolized Ag NPs (20 nm [C20] or 110 nm [C110] Ag NPs) for 6 hr. Bronchoalveolar
lavage fluid (BALF) and lung tissues were obtained at 1, 7, 21, and 56 days
postexposure for analyses. Inhalation of Ag NPs, versus citrate buffer control,
produced significant inflammatory and cytotoxic responses that were measured in
BALF cells and supernatant. At day 7, total cells, protein, and lactate
dehydrogenase were significantly elevated in BALF, and peak histopathology was
noted after C20 or C110 exposure versus control. At day 21, BALF polymorphonuclear
cells and tissue inflammation were significantly greater after C20 versus C110
exposure. By day 56, inflammation was resolved in Ag NP-exposed animals. Overall,
results suggest delayed, short-lived inflammatory and cytotoxic effects following
C20 or C110 inhalation and potential for greater responses following C20 exposure.
AN - rayyan-553781376
AU - Silva, R. M.
AU - Anderson, D. S.
AU - Peake, J.
AU - Edwards, P. C.
AU - Patchin, E. S.
AU - Guo, T.
AU - Gordon, T.
AU - Chen, L. C.
AU - Sun, X. L.
AU - Van Winkle, L. S.
AU - Pinkerton, K. E.
DO - 10.1177/0192623316629804
IS - 5
KW - Aerosols
Lung
Rats
Reaction Time
PY - 2016
SN - 0192-6233 1533-1601
SP - 673-686
ST - Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over
Time
T2 - TOXICOLOGIC PATHOLOGY
TI - Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over
Time
VL - 44
Y2 - 7
ID - 9318
ER -
TY - JOUR
AB - Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most widely used
drugs for the suppression of inflammation and pain. However, the analgesic
properties of NSAIDs are also associated with significant negative side effects,
most notably in the gastrointestinal (GI) tract. Increasingly, evidence indicates
that the ulcerogenic properties of some NSAIDs are not exclusively the result of
inhibition of cyclooxygenase isoforms in the GI tract, and other mechanisms,
including inhibition of cell migration and epithelial restitution, are being
explored. Recently, microarray analysis was used to identify potential novel
targets of NSAID activity in intestinal epithelial cells. Treated cells exhibited
significant reductions in the gene expression of pleiotrophin (PTN), a cytokine and
growth factor known to participate in angiogenesis and bone growth. This report
aimed to confirm the microarray results reported previously, and to measure protein
expression of PTN in intestinal epithelial cells. Furthermore, we also examined the
effects of exogenous PTN on cell migration in the presence and absence of either
NSAIDs with variable ulcerogenic potential or PTN-specific siRNA. Our results
demonstrated that indomethacin and NS-398, two NSAIDs with ulcerogenic potential
significantly decrease both gene and protein expressions of PTN in IEC-6 cells and
protein expression in IEC-6-Cdx2 cells. Additionally, cell migration experiments
with PTN siRNA showed that PTN is an important mediator of IEC-6 cell migration,
and addition of exogenous PTN partially restores the deficits in cell migration
caused by treatment with indomethacin and NS-398. Finally, measurement of PTN
protein expression in the GI tract of horses treated with phenylbutazone showed
that PTN expression is reduced by NSAIDs in vivo. Our results show that PTN is an
important mediator of cell migration in IEC-6 cells, and PTN is a potential target
through which NSAIDs may inhibit cell migration, epithelial restitution, and wound
healing in the GI tract.
AN - rayyan-553781380
AU - Silver, K.
AU - Desormaux, A.
AU - Freeman, L. C.
AU - Lillich, J. D.
DO - 10.3109/08977194.2012.693920
IS - 4
PY - 2012
SN - 0897-7194 1029-2292
SP - 258-266
ST - Expression of pleiotrophin, an important regulator of cell migration, is
inhibited in intestinal epithelial cells by treatment with non-steroidal anti-
inflammatory drugs
T2 - GROWTH FACTORS
TI - Expression of pleiotrophin, an important regulator of cell migration, is
inhibited in intestinal epithelial cells by treatment with non-steroidal anti-
inflammatory drugs
VL - 30
Y2 - 8
ID - 9321
ER -
TY - JOUR
AB - The eosinophilia-myalgia syndrome (EMS) is a recently described disease that
has been associated with the ingestion of L-tryptophan containing trace amounts of
several impurities. The first such contaminant to be identified and linked
epidemiologically to the EMS epidemic was 1,1'-ethylidenebis(L-tryptophan) (EBT),
but its role in the etiology and pathogenesis of the syndrome has been
controversial. We report the development of inflammation and fibrosis affecting the
dermis and subcutis, including the fascia and perimyseal tissues, after the daily
intraperitoneal administration of EBT to female C57BL/6 mice. Such changes are
accompanied by increased numbers of mast cells, many of which appear to be
degranulating. Plasma levels of quinolinic acid, a metabolic product of L-
tryptophan via the kynurenine pathway, are reduced initially, and then become
elevated when inflammation and fibrosis are more pronounced. The nature and
location of the inflammatory cell infiltrate and fibrosis, as well as the presence
of mast cells and alterations of L-tryptophan metabolism, are consistent with
findings reported in patients with EMS. This murine model suggests that EBT may
have been one of the mediators of EMS and should facilitate studies of the
pathogenesis of EMS.
AN - rayyan-553782190
AU - Silver, R. M.
AU - Ludwicka, A.
AU - Hampton, M.
AU - Ohba, T.
AU - Bingel, S. A.
AU - Smith, T.
AU - Harley, R. A.
AU - Maize, J.
AU - Heyes, M. P.
DO - 10.1172/JCI117125
IS - 4
J2 - J Clin Invest
KW - Animals
Eosinophilia-Myalgia Syndrome/*chemically induced/pathology
Fascia/pathology
Female
Mice
Mice, Inbred C57BL
Muscles/pathology
Quinolinic Acid/blood
Tryptophan/*analogs & derivatives/toxicity
Tryptophan
Eosinophilia
LA - eng
N1 - Department of Medicine, Medical University of South Carolina, Charleston
29425.
PY - 1994
SN - 0021-9738 (Print)
SP - 1473-80
ST - A murine model of the eosinophilia-myalgia syndrome induced by 1,1'-
ethylidenebis (L-tryptophan)
T2 - The Journal of clinical investigation
TI - A murine model of the eosinophilia-myalgia syndrome induced by 1,1'-
ethylidenebis (L-tryptophan)
VL - 93
Y2 - 4
ID - 10100
ER -
TY - JOUR
AB - This review covers the most recent developments of inorganic and organic-
inorganic composite coatings for orthopedic implants, providing the interface with
living tissue and with potential for drug delivery to combat infections.
Conventional systemic delivery of drugs is an inefficient procedure that may cause
toxicity and may require a patient's hospitalization for monitoring. Local delivery
of antibiotics and other bioactive molecules maximizes their effect where they are
required, reduces potential systemic toxicity and increases timeliness and cost
efficiency. In addition, local delivery has broad applications in combating
infection-related diseases. Polymeric coatings may present some disadvantages.
These disadvantages include limited chemical stability, local inflammatory
reactions, uncontrolled drug-release kinetics, late thrombosis and restenosis. As a
result, embedding of bioactive compounds and biomolecules within inorganic coatings
(bioceramics, bioactive glasses) is attracting significant attention. Recently
nanoceramics have attracted interest because surface nanostructuring allows for
improved cellular adhesion, enhances osteoblast proliferation and differentiation,
and increases biomineralization. Organic-inorganic composite coatings, which
combine biopolymers and bioactive ceramics that mimick bone structure to induce
biomineralization, with the addition of biomolecules, represent alternative systems
and ideal materials for "smart" implants. In this review, emphasis is placed on
materials and processing techniques developed to advance the therapeutic use of
biomolecules-eluting coatings, based on nanostructured ceramics. One part of this
report is dedicated to inorganic and composite coatings with antibacterial
functionality. From the Clinical Editor: Inorganic and composite nanotechnology-
based coating methods have recently been developed for orthopedic applications,
with the main goal to provide bactericide and other enhanced properties, which may
result in reduced need for pharmaceutical interventions and overall more cost
effective orthopedic procedures. This review discusses key aspects of the above
developments. © 2011 Elsevier Inc.
AN - rayyan-553781384
AU - Simchi, A.
AU - Tamjid, E.
AU - Pishbin, F.
AU - Boccaccini, A. R.
DO - 10.1016/j.nano.2010.10.005
IS - 1
KW - Antimicrobial
Ceramics
Coatings
Composites
Drug-eluting implants
Nanostructures
Humans
Nanotechnology
Bactericides
Bioactive glass
Bioceramics
Biomineralization
Biomolecules
Biopolymers
Chemical stability
Cost effectiveness
Cost reduction
Drug delivery
Inorganic coatings
Mammals
Microorganisms
Ocean habitats
Organic coatings
Toxicity
antibiotic agent
bioceramics
biomaterial
biopolymer
cefalotin
cefamandole
doxorubicin
drug carrier
gentamicin
glass
hydroxyapatite
inorganic compound
macrogol
nanocoating
nanocomposite
nanofilm
nitric oxide
organic compound
polyglactin
polylactic acid
silver
titanium dioxide
tobramycin
vancomycin
Alternative systems
Bioactive ceramic
Bioactive compounds
Bioactive molecules
Bone structure
Broad application
Cellular adhesion
Coating methods
Cost effective
Cost efficiency
Living tissues
Local delivery
Nano ceramics
Nanostructured ceramic
Organic-inorganic composite
Orthopaedic applications
Orthopedic applications
Orthopedic implant
Osteoblast proliferation
Polymeric coatings
Processing technique
Recent progress
Release kinetics
Restenosis
Surface nanostructuring
Therapeutic use
biomineralization
cell adhesion
cell proliferation
composite material
cost
human
material coating
nonhuman
orthopedic equipment
osteoblast
review
Composite coatings
PY - 2011
SP - 22-39
ST - Recent progress in inorganic and composite coatings with bactericidal
capability for orthopaedic applications
TI - Recent progress in inorganic and composite coatings with bactericidal
capability for orthopaedic applications
78751697635&doi=10.1016%2fj.nano.2010.10.005&partnerID=40&md5=eab8583a51916c27e842b
39ac9d5e28a
VL - 7
ID - 9325
ER -
TY - JOUR
AB - The highly conserved matrix protein 2 (M2) is a good candidate for the
development of a broadly protective influenza vaccine that induces long-lasting
immunity. In animal models, natural killer (NK) cells have been proposed to play an
important role in the protection provided by M2-based vaccines through a mechanism
of antibody-dependent cell-mediated cytotoxicity (ADCC). We investigated the
ability of the human anti-M2 Ab1-10 monoclonal antibody (mAb) to activate human NK
cells. They mediated ADCC against M2-expressing cells in the presence of Ab1-10
mAb. Furthermore, NK cell pro-inflammatory cytokine and chemokine secretion is also
enhanced when Ab1-10 mAb is present. We also generated cytokine-preactivated NK
cells and showed that they still displayed increased effector functions in the
presence of Ab1-10 mAb. Thus, our study has demonstrated that human resting and
cytokine-preactivated NK cells may have a very important role in the protection
provided by anti-M2 Abs.
AN - rayyan-553781385
AU - Simhadri, V. R.
AU - Dimitrova, M.
AU - Mariano, J. L.
AU - Zenarruzabeitia, O.
AU - Zhong, W. M.
AU - Ozawa, T.
AU - Muraguchi, A.
AU - Kishi, H.
AU - Eichelberger, M. C.
AU - Borrego, F.
DO - 10.1371/journal.pone.0124677
IS - 4
KW - Humanism
Humans
Humanities
Cytokines
PY - 2015
SN - 1932-6203
ST - A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated
Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated
Natural Killer (NK) Cells
T2 - PLOS ONE
TI - A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated
Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated
Natural Killer (NK) Cells
VL - 10
Y2 - 4 y3 - 27
ID - 9326
ER -
TY - JOUR
AB - The present study describes potential beneficial and adverse effects of
plant-extract synthesized gold nanoparticles (AuNPs) on ethanol toxicity in SH-SY5Y
cells. Although kudzu root extract (K), edible-gum extract (G), alone or in
combination (KG), reduced Au3+ into AuNPs, the extract's composition and the
reaction temperature determined their size (AuNPKG(90<50<37) << AuNPK (90,50<37) <
AuNPG (90<50); the subscript KG, K, or G is extract identification and numerical
vales are reaction temperature in Celsius) and biological properties (AuNPKG
(90,50>37) << AuNPK ((90,50>37)) < AuNPG (90,50)). The surface of each AuNP
contained the extract's active ingredients, that were analyzed and confirmed using
laser desorption ionization (LDI)) and low-matrix laser desorption-ionization
((L)MALDI). AuNPKG-50 was (i) least toxic to SH-SY5Y cells, but most effective in
suppressing the adverse effects of ethanol on SH-SY5Y cells, and (ii) more
effective than a combination of free kudzu and gum extracts. The beneficial and
adverse effects of AuNPs may have been modified by the formation of proteins
corona. This study provides a proof of concept for possible application of plant-
extract synthesized AuNPs in mitigating ethanol toxicity.
AN - rayyan-553781386
AU - Singh, A. K.
DO - 10.3390/biomedicines5040070
IS - 4
PY - 2017
SN - 2227-9059
ST - Comparative Therapeutic Effects of Plant-Extract Synthesized and
Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory
Activity in SH-SY5Y Cells In Vitro
T2 - BIOMEDICINES
TI - Comparative Therapeutic Effects of Plant-Extract Synthesized and
Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory
Activity in SH-SY5Y Cells In Vitro
VL - 5
Y2 - 12
ID - 9327
ER -
TY - JOUR
AB - The present study investigates a simple and convenient one-step procedure for
the preparation of bovine serum albumin (BSA)-Rh2 nanoparticles (NPs) at room
temperature. In this work, ginsenoside Rh2 was entrapped within the BSA protein to
form BSA-Rh2 NPs to enhance the aqueous solubility, stability, and therapeutic
efficacy of Rh2. The physiochemical characterization by high-performance liquid
chromatography, nuclear magnetic resonance, Fourier transform infrared
spectroscopy, field emission transmission electron microscopy, dynamic light
scattering, and thermogravimetric analysis confirmed that the prepared BSA-Rh2 NPs
were spherical, highly monodispersed, and stable in aqueous systems. In addition,
the stability of NPs in terms of different time intervals, pHs, and temperatures
(20 degrees C-700 degrees C) was analyzed. The results obtained with different pHs
showed that the synthesized BSA-Rh2 NPs were stable in the physiological buffer (pH
7.4) for up to 8 days, but degraded under acidic conditions (pH 5.0) representing
the pH inside tumor cells. Furthermore, comparative analysis of the water
solubility of BSA-Rh2 NPs and standard Rh2 showed that the BSA nanocarrier enhanced
the water solubility of Rh2. Moreover, in vitro cytotoxicity assays including cell
viability assays and morphological analyses revealed that Rh2-entrapped BSA NPs,
unlike the free Rh2, demonstrated better in vitro cell viability in HaCaT skin cell
lines and that BSA enhanced the anticancer effect of Rh2 in A549 lung cell and HT29
colon cancer cell lines. Additionally, anti-inflammatory assay of BSA-Rh2 NPs and
standard Rh2 performed using RAW264.7 cells revealed decreased lipopolysaccharide-
induced nitric oxide production by BSA-Rh2 NPs. Collectively, the present study
suggests that BSA can significantly enhance the therapeutic behavior of Rh2 by
improving its solubility and stability in aqueous systems, and hence, BSA-Rh2 NPs
may potentially be used as a ginsenoside delivery vehicle in cancer and
inflammatory cell lines.
AN - rayyan-553781390
AU - Singh, P.
AU - Kim, Y. J.
AU - Singh, H.
AU - Ahn, S.
AU - Castro-Aceituno, V.
AU - Yang, D. C.
DO - 10.2147/IJN.S125154
KW - Serum Albumin
Serum Albumin, Bovine
PY - 2017
SN - 1178-2013
SP - 4073-4084
ST - In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum
albumin nanoparticles: in vitro cytocompatibility studies
TI - In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum
albumin nanoparticles: in vitro cytocompatibility studies
VL - 12
ID - 9331
ER -
TY - JOUR
AB - Previously, we showed the rapid and eco-friendly synthesis of gold and silver
nanoparticles within 3 and 45min by fresh leaves extract of herbal medicinal plant
Panax ginseng. In addition, we characterized the nanoparticles in terms of shape,
size, morphology and stability by FE-TEM, EDX, elemental mapping, SEAD, XRD and
particles size analysis. In addition of this, we showed their antimicrobial, anti-
coagulant, and biofilm inhibition activity of nanoparticles. Continuing our
previous study, here we highlight the further characterization and biomedical
applications of P. ginseng leaf-mediated gold and silver nanoparticles. We
characterized the nanoparticles further in terms of active functional group and
capping layer, surface charge, and temperature stability. Based on these factors,
we explored the nanoparticles for antioxidant efficacy, biocompatibility in HaCaT
cells, 3T3-L1 pre-adipocytes cells, for anticancer efficacy in A549 lung cancer and
B16BL6 skin melenoma cancer cell lines and for anti-inflammation efficacy in RAW
264.7 cell lines. Based on our findings, we suggest that the P. ginseng-mediated
gold nanoparticles have high antioxidant activity and highly biocompatibility in
HaCaT cells, 3T3-L1 pre-adipocytes cells, RAW 264.7 cells lines and could be
considered for future drug delivery carriers. The silver nanoparticles also showed
high potent antioxidant efficacy, additionally it showed high anticancer effect in
A549 lung cancer and B16BL6 skin melenoma cancer cell lines as compared to
precursor salts. Moreover, both gold and silver nanoparticles have anti-
inflammatory efficacies in RAW 264.7 cells. Thus, the study may provide useful
insights of P. ginseng leaves extract-mediated biocompatible gold and silver
nanoparticles and improving their applicability in designing nanoparticles carrier
systems for drug delivery applications.
AN - rayyan-553781392
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Jimenez, Z.
AU - Simu, S. Y.
AU - Kim, Y. J.
AU - Yang, D. C.
DO - 10.1080/21691401.2016.1243547
IS - 7
KW - Panax
PY - 2017
SN - 2169-1401 2169-141X
SP - 1415-1424
ST - Pharmacological importance, characterization and applications of gold and
silver nanoparticles synthesized by Panax ginseng fresh leaves
T2 - ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
TI - Pharmacological importance, characterization and applications of gold and
VL - 45
ID - 9333
ER -
TY - JOUR
AB - Previously, we showed the rapid and eco-friendly synthesis of gold and silver
nanoparticles within 3 and 45 min by fresh leaves extract of herbal medicinal plant
Panax ginseng. In addition, we characterized the nanoparticles in terms of shape,
size, morphology and stability by FE-TEM, EDX, elemental mapping, SEAD, XRD and
particles size analysis. In addition of this, we showed their antimicrobial, anti-
coagulant, and biofilm inhibition activity of nanoparticles. Continuing our
previous study, here we highlight the further characterization and biomedical
applications of P. ginseng leaf-mediated gold and silver nanoparticles. We
characterized the nanoparticles further in terms of active functional group and
capping layer, surface charge, and temperature stability. Based on these factors,
we explored the nanoparticles for antioxidant efficacy, biocompatibility in HaCaT
cells, 3T3-L1 pre-adipocytes cells, for anticancer efficacy in A549 lung cancer and
B16BL6 skin melenoma cancer cell lines and for anti-inflammation efficacy in RAW
264.7 cell lines. Based on our findings, we suggest that the P. ginseng-mediated
gold nanoparticles have high antioxidant activity and highly biocompatibility in
HaCaT cells, 3T3-L1 pre-adipocytes cells, RAW 264.7 cells lines and could be
considered for future drug delivery carriers. The silver nanoparticles also showed
high potent antioxidant efficacy, additionally it showed high anticancer effect in
A549 lung cancer and B16BL6 skin melenoma cancer cell lines as compared to
precursor salts. Moreover, both gold and silver nanoparticles have anti-
inflammatory efficacies in RAW 264.7 cells. Thus, the study may provide useful
insights of P. ginseng leaves extract-mediated biocompatible gold and silver
nanoparticles and improving their applicability in designing nanoparticles carrier
systems for drug delivery applications.
AN - rayyan-553781857
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Jiménez, Z.
AU - Simu, S. Y.
AU - Kim, Y. J.
AU - Yang, D. C.
DO - 10.1080/21691401.2016.1243547
IS - 7
J2 - Artif Cells Nanomed Biotechnol
KW - 3T3-L1 Cells
Animals
Biphenyl Compounds/chemistry
Chemistry Techniques, Synthetic
Gold/*chemistry/pharmacology
Humans
Materials Testing
Mice
Nanotechnology
Nitric Oxide/biosynthesis
Panax/*chemistry
Picrates/chemistry
Plant Leaves/*chemistry
Silver/*chemistry/pharmacology
Panax
LA - eng
N1 - a Department of Oriental Medicine Biotechnology , Ginseng Bank College of
Life Science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.;
b Graduate School of Biotechnology College of life science, Kyung Hee University ,
Yongin-si , Gyeonggi-do , Republic of Korea.; a Department of Oriental Medicine
Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University ,
Yongin-si , Gyeonggi-do , Republic of Korea.; b Graduate School of Biotechnology
College of life science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic
of Korea.; b Graduate School of Biotechnology College of life science, Kyung Hee
University , Yongin-si , Gyeonggi-do , Republic of Korea.; a Department of Oriental
Medicine Biotechnology , Ginseng Bank College of Life Science, Kyung Hee University
, Yongin-si , Gyeonggi-do , Republic of Korea.; a Department of Oriental Medicine
Yongin-si , Gyeonggi-do , Republic of Korea.; b Graduate School of Biotechnology
College of life science, Kyung Hee University , Yongin-si , Gyeonggi-do , Republic
of Korea.
PY - 2017
SP - 1415-1424
ST - Pharmacological importance, characterization and applications of gold and
T2 - Artificial cells, nanomedicine, and biotechnology
TI - Pharmacological importance, characterization and applications of gold and
VL - 45
Y2 - 11
ID - 9782
ER -
TY - JOUR
AB - The current study highlights the fabrication of drug delivery system by
utilizing 200 nm mesoporous silica nanoparticles (MSNPs) with 4-nm pore size, as a
carrier system for delivery ginsenoside compound K (CK) and Rh2 to enhance their
efficacy. The two pharmacologically imperative ginsenosides, CK and Rh2, were
loaded to the MSNPs to prepare MSNPs-CK and MSNPs-Rh2, respectively. A fluorescein
isothiocyanate (FITC) fluorescent dye was combined in the MSNPs carrier system, in
order to trace the cellular uptake of ginsenoside-loaded nanoparticles for in vitro
studies. Following purification, the so-prepared MSNPs-CK-FITC and MSNPs-Rh2-FITC
were characterized by several analytical techniques, which includes, high-pressure
liquid chromatography (HPLC), H-1 NMR, field emission transmission electron
microscopy (FE-TEM), Fourier transform infrared spectroscopy (FT-IR), x-ray
diffraction (XRD), thermogravimetric analysis (TGA), and dynamic light scattering
(DLS). In vitro cytotoxicity assay in HaCaT skin cells, A549 lung cancer cells,
HepG2 liver carcinoma cells, and HT-29 colon cancer cell lines were tested for
MSNPs-CK-FITC and MSNPs-Rh2-FITC. The results demonstrate the excellent
biocompatibility of nanoparticles in normal cell lines (HaCaT skin cells) and
anticancer efficacy in all the tested cancer cell lines at 10-mu M concentration.
Additionally, the in vitro anti-inflammatory behavior of MSNPs-CK-FITC and MSNPs-
Rh2-FITC were checked in RAW264.7 (murine macrophage) cell lines. The outcomes
showed higher anti-inflammatory efficacy of MSNPs-CK-FITC and MSNPs-Rh2-FITC as
compared to standard ginsenosides CK and Rh2 in RAW264.7 cell lines. Thus, with 200
nm MSNPs carrier system for the delivery ginsenosides CK and Rh2, a high amount of
loading and increasing in vitro pharmacological efficacies of ginsenosides were
realized. This study may provide useful insights for designing and improving the
applicability of MSNPs for ginsenoside delivery.
AN - rayyan-553781393
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Kim, Y. J.
AU - Farh, M. E.
AU - Yang, D. C.
DO - 10.1007/s11051-017-3949-9
IS - 7
PY - 2017
SN - 1388-0764 1572-896X
ST - Engineering of mesoporous silica nanoparticles for release of ginsenoside CK
and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro
studies
TI - Engineering of mesoporous silica nanoparticles for release of ginsenoside CK
and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro
studies
VL - 19
Y2 - 7 y3 - 20
ID - 9334
ER -
TY - JOUR
AB - Ginsenosides are triterpenoids that are found in P. ginseng; they have
numerous important structural, functional and pharmacological properties. In this
work, a desolvation method was used to entrap ginsenoside CK within bovine serum
albumin (BSA) to form BSA-CK nanoparticles (NPs), which enhance its aqueous
solubility and stability. Following purification, the BSA-CK NPs were characterized
by several physico-chemical techniques, including high pressure liquid
chromatography (HPLC), electrophoresis, H-1 NMR spectroscopy, Fourier transform
infrared spectroscopy (FT-IR), field emission transmission electron microscopy (FE-
TEM), zeta potential, particle size analysis by dynamic light scattering (DLS), and
thermogravimetric analysis (TGA); the results confirm that the as-prepared BSA-CK
NPs are spherical, highly monodispersed and stable in aqueous systems. In addition,
the time-dependent and pH stabilities of the BSA-CK NPs were analyzed over a period
of 8 days; the results suggest that the nanoparticles are stable in physiological
buffer (pH 7.4), whereas they are readily degraded under acidic conditions (pH 5.0)
which mimic intracellular pH conditions. Furthermore, comparative water solubility
analysis of the BSA-CK NPs and standard CK showed that the BSA carrier enhances the
water solubility of ginsenoside CK. In vitro cytotoxicity assays of the BSA-CK NPs
and standard CK revealed that the BSA-CK NPs demonstrate greater in vitro
therapeutic efficacy in the HaCaT skin cell line, the A549 lung cancer cell line,
the HepG2 liver carcinoma cell line and the HT29 colon cancer cell line in
comparison with standard CK. Moreover, RAW264.7 cells treated with BSA-CK NPs
exhibited decreased lipopolysaccharide-induced NO production. Collectively, these
results suggest that the BSA-CK NPs may be useful as a delivery vehicle in cancer
cell lines and may also possess anti-inflammatory effects.
AN - rayyan-553781394
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Kim, Y. J.
AU - Yang, D. C.
DO - 10.1039/c6ra25264h
IS - 25
KW - Serum Albumin
PY - 2017
SN - 2046-2069
SP - 15397-15407
ST - Bovine serum albumin as a nanocarrier for the efficient delivery of
ginsenoside compound K: preparation, physicochemical characterizations and in vitro
biological studies
T2 - RSC ADVANCES
TI - Bovine serum albumin as a nanocarrier for the efficient delivery of
ginsenoside compound K: preparation, physicochemical characterizations and in vitro
biological studies
VL - 7
ID - 9335
ER -
TY - JOUR
AB - Background: Acacetin is a di-hydroxy and mono-methoxy flavone present in
various plants, including black locust, Damiana, Silver birch. Literature
information revealed that acacetin exhibits an array of pharmacological potential
including chemopreventive and cytotoxic properties in cancer cell lines, prevents
ischemia/reperfusion/myocardial infarction-induced cardiac injury,
lipopolysaccharide (LPS), 1-methyl-4-phenyl pyridinium ion (MPP+) and 1-methyl-4-
phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP)-induced neuroinflammation,
LPS and sepsis-induced lung injury, rheumatoid and collagen-induced arthritis,
inhibit the microbial growth, obesity, viral-mediated infections as well as hepatic
protection. Purpose: This review highlights the therapeutic potential of acacetin,
with updated and comprehensive information on the biological sources, chemistry,
and pharmacological properties along with the possible mechanism of action, safety
aspects, and future research opportunities. Study design: The information was
retrieved from various search engines, including Pubmed, SciFinder, Science direct,
Inxight:drugs, Google scholar, and Meta cyc. Result: The first section of this
review focuses on the detailed biological source of acacetin, chromatographic
techniques used for isolation, chemical characteristics, the method for the
synthesis of acacetin, and the available natural and synthetic derivatives.
Subsequently, the pharmacological activities, including anti-cancer, anti-
inflammatory, anti-viral, anti-microbial, anti-obesity, have been discussed. The
pharmacokinetics data and toxicity profile of acacetin are also discussed.
Conclusion: Acacetin is a potent molecule reported for its strong anti-inflammatory
and anti-cancer activity, however further scientific evidence is essential to
validate its potency in disease models associated with inflammation and cancer.
There is limited information available for toxicity profiling of acacetin;
therefore, further studies would aid in establishing this natural flavone as a
potent candidate for research studies at clinical setup. © 2020 Elsevier Ltd
AN - rayyan-553781396
AU - Singh, S.
AU - Gupta, P.
AU - Meena, A.
AU - Luqman, S.
DO - 10.1016/j.fct.2020.111708
KW - Acacetin
Anti-cancer
Antioxidants
Flavone
Natural product
Pharmacological activity
Animals
Antimetabolites
Flavones
Humans
Inflammation
Metabolic Diseases
Neoplasms
acacetin
antimetabolite
flavone derivative
acute lung injury
antiaging activity
antileishmanial activity
antimalarial activity
antinociception
antiobesity activity
antipyretic activity
antiviral activity
Article
biological activity
chemistry
chromatography
clinical research
drug cytotoxicity
drug efficacy
drug isolation
drug safety
drug structure
drug synthesis
evidence based practice
heart protection
human
hypouricemia
infection
inflammation
metabolic disorder
neuroprotection
nonhuman
vasodilatation
animal
neoplasm
PY - 2020
ST - Acacetin, a flavone with diverse therapeutic potential in cancer,
inflammation, infections and other metabolic disorders
TI - Acacetin, a flavone with diverse therapeutic potential in cancer,
inflammation, infections and other metabolic disorders
85090599665&doi=10.1016%2fj.fct.2020.111708&partnerID=40&md5=1b47c067137a51c4caa38c
9fd091f273
VL - 145
ID - 9337
ER -
TY - JOUR
AB - Globally, high death rates and poor quality of life are caused mainly by
inflammatory diseases. Corticosteroids, which may have systemic side effects and
would enhance the risk of infection, are the common forms of therapy. The field of
nanomedicine has created composite nanoparticles that carry a pharmacological
carrier and target ligands for distribution to sites of inflammation with less
systemic toxicity. However, their relatively large size often causes systemic
clearance. An interesting approach is metal-based nanoparticles that naturally
reduce inflammation. They are made not only to be small enough to pass through
biological barriers but also to allow label-free monitoring of their interactions
with cells. The following literature review discusses the mechanistic analysis of
the anti-inflammatory properties of several metal-based nanoparticles, including
gold, silver, titanium dioxide, selenium, and zinc oxide. Current research focuses
on the mechanisms by which nanoparticles infiltrate cells and the anti-inflammatory
techniques using herbal extracts-based nanoparticles. Additionally, it provides a
brief overview of the literature on many environmentally friendly sources employed
in nanoparticle production and the mechanisms of action of various nanoparticles.
AN - rayyan-553782305
AU - Singh, S.
AU - Sharma, K.
AU - Sharma, H.
DO - 10.2174/1567201820666230602164325
J2 - Curr Drug Deliv
LA - eng
N1 - Institute of Pharmaceutical Research GLA University, 17km Stone, NH-2,
Mathura-Delhi Road Mathura, Chaumuhan, Uttar Pradesh-281406, India.; Institute of
Pharmaceutical Research GLA University, 17km Stone, NH-2, Mathura-Delhi Road
Mathura, Chaumuhan, Uttar Pradesh-281406, India.; Department of Computer
Engineering & Applications GLA University, 17km Stone, NH-2, Mathura-Delhi Road
Mathura, Chaumuhan, Uttar Pradesh-281406, India.
PY - 2023
ST - Green Extracts with Metal-Based Nanoparticles for Treating Inflammatory
Diseases: A Review
T2 - Current drug delivery
TI - Green Extracts with Metal-Based Nanoparticles for Treating Inflammatory
Diseases: A Review
Y2 - 6 y3 - 2
ID - 10213
ER -
TY - JOUR
AB - The ever increasing incidences of non-healing skin wounds have paved way for
many efforts on the convoluted process of wound healing. Unfortunately, the lack of
relevance and success of modern wound dressings in healing of acute and diabetic
wounds still remains a matter of huge concern. Here, an in situ three step approach
was embraced for the development of nanocomposite (NCs) dressings by impregnating
silver nanoparticles (AgNPs) onto a matrix of cellulose nanocrystals (CNCs)
isolated from Syzygium cumini leaves using an environmental friendly approach.
Topical application of NCs (ointments and strips) on acute and diabetic wounds of
mice documented enhanced tissue repair (~99% wound closure) via decrease in
inflammation; increase in angiogenesis, collagen deposition, and rate of neo-
epithelialization that ultimately led to formation of aesthetically sound skin in
lesser time than controls. Due to the synergistic action of CNCs (having high water
uptake capacity) and AgNPs (anti-microbial agents), NCs tend to increase the
expression of essential growth factors (FGF, PDGF and VEGF) and collagen while
decreasing the pro-inflammatory factors (IL-6 and TNF-α) at the same time, thus
accelerating healing. The results suggested the potential of these developed anti-
microbial, cytocompatible and nanoporous NCs having optimized AgNPs concentration
as ideal dressings for effective wound management.
AN - rayyan-553781998
AU - Singla, R.
AU - Soni, S.
AU - Patial, V.
AU - Kulurkar, P. M.
AU - Kumari, A.
AU - S, M.
AU - Padwad, Y. S.
AU - Yadav, S. K.
DO - 10.1038/s41598-017-08897-9
IS - 1
J2 - Sci Rep
KW - Animals
*Anti-Infective Agents/chemistry
*Bandages
Cell Survival
*Cellulose/chemistry
Collagen/metabolism
Diabetes Complications/therapy
Materials Testing
Mechanical Phenomena
Mice
Nanocomposites/chemistry/ultrastructure
*Nanoparticles/chemistry/ultrastructure
*Silver/chemistry
Spectrum Analysis
Syzygium/*chemistry
*Wound Healing
Wound Healing
Cellulose
LA - eng
N1 - Nanobiology Lab, Biotechnology Division, CSIR-Institute of Himalayan
Bioresource Technology, Palampur (H.P.), 176061, India.; Academy of Scientific and
Innovative Research (AcSIR), CSIR-IHBT, Palmapur, India.; Pharmacology and
Toxicology Lab, Food and Nutraceuticals Division, CSIR-Institute of Himalayan
Bioresource Technology, Palampur (H.P.), 176061, India.; Nanobiology Lab,
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology,
Palampur (H.P.), 176061, India.; Academy of Scientific and Innovative Research
(AcSIR), CSIR-IHBT, Palmapur, India.; Pharmacology and Toxicology Lab, Food and
Nutraceuticals Division, CSIR-Institute of Himalayan Bioresource Technology,
Palampur (H.P.), 176061, India.; Pharmacology and Toxicology Lab, Food and
Nutraceuticals Division, CSIR-Institute of Himalayan Bioresource Technology,
Palampur (H.P.), 176061, India. yogendra@ihbt.res.in.; Academy of Scientific and
Innovative Research (AcSIR), CSIR-IHBT, Palmapur, India. yogendra@ihbt.res.in.;
Nanobiology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource
Technology, Palampur (H.P.), 176061, India. sudesh@ciab.res.in.; Academy of
Scientific and Innovative Research (AcSIR), CSIR-IHBT, Palmapur, India.
sudesh@ciab.res.in.; Center of Innovative and Applied Bioprocessing (CIAB),
Knowledge City, Sector-81, Mohali, 140306, India. sudesh@ciab.res.in.
PY - 2017
SP - 10457
ST - Cytocompatible Anti-microbial Dressings of Syzygium cumini Cellulose
Nanocrystals Decorated with Silver Nanoparticles Accelerate Acute and Diabetic
Wound Healing
TI - Cytocompatible Anti-microbial Dressings of Syzygium cumini Cellulose
Nanocrystals Decorated with Silver Nanoparticles Accelerate Acute and Diabetic
Wound Healing
VL - 7
Y2 - 9 y3 - 5
ID - 9915
ER -
TY - JOUR
AB - The objective of the present study was to elucidate general and local
characteristics of the tissue reactions to the implantation of radiation-modified
polytetrafluoroethylene (PTFE)-based fluoroplast F-4PM20 with a diamond-like carbon
(DLC) nanocoating or with the diamond-like carbon coating containing the dispersed
nano-sized silver particles to the experimental animals (rats). A total of 150
inbred white rats were included into the experiment; they were divided into 3
groups comprised of 50 animals each. The rats in group 1 were implanted with the 5
nm thick strips of fluoroplast F-4PM20 having the diamond-like carbon nanocoating.
The animals of group 2 were implanted with the same material containing
nanoparticles of chemically pure silver dispersed in the coating, those in group 3
(controls) were implanted with the fluoroplast F-4PM20 without a coating. The
animals were sacrificed on days 7, 21, 30, and 60 days after the onset of the
experiment. The tissues surrounding the implant as well as heart, lung, spleen,
liver, and kidney tissues were taken for the histological study. The local
reactions of different tissues were found to be uniform even though there was an
apparent tendency toward the less pronounced granulation and scarification
processes in the animals implanted with the diamond-like carbon coating containing
the dispersed nano-sized silver particles. In none of the groups, the animals
exhibited statistically significant lymphoid tissue hyperplasia in the spleen which
suggested the activation of the immune system in response to implantation. It is
concluded that the PTFE-based fluoroplast F-4PM20 implants with the 5 nm thick DLC
coating and a similar coating containing the dispersed nano-sized silver particles
can be applied for middle ear reconstructive surgery, being a histologically
compatible material that does not cause an inflammatory degenerative response of
the tissues.
AN - rayyan-553782266
AU - Sitnikov, V. P.
AU - Shil'ko, S. V.
AU - Khusam É, R.
AU - Nadyrov É, A.
AU - Kazachenko, V. P.
AU - Dzhaĭnakbaev, N. T.
IS - 3
J2 - Vestn Otorinolaringol
KW - Animals
Coated Materials, Biocompatible/therapeutic use
Foreign-Body Reaction/etiology/*pathology
Materials Testing/methods
Nanodiamonds/*therapeutic use
Ossicular Prosthesis/*adverse effects
*Ossicular Replacement/adverse effects/instrumentation/methods
Polytetrafluoroethylene/*therapeutic use
Rats
Silver/*therapeutic use
Treatment Outcome
Ear, Inner
Ear
Polytetrafluoroethylene
LA - rus
PY - 2014
SN - 0042-4668 (Print)
SP - 20-3
ST - [The possibilities for the application of the fluoroplast-based prostheses
with a diamond-like carbon nanocoating in ear surgery (an experimental study)]
T2 - Vestnik otorinolaringologii
TI - [The possibilities for the application of the fluoroplast-based prostheses
with a diamond-like carbon nanocoating in ear surgery (an experimental study)]
ID - 10176
ER -
TY - JOUR
AB - Green silver nanoparticles have received much interest over the years because
they are cheap, good for the environment, and easy to use. Present study, first
report to synthesized silver nanoparticles from the leaf extract of Andrographis
macrobotrys, which reduces AgNO3 into Ag through the presence of phytochemicals.
The nanoparticles were examined using (UV, spec, FTIR, XRD, TEM and EDAX. The dark
brown colour of the A. macrobotrys colloidal showed maximum absorbance at 450 nm.
The TEM images displayed synthesised nanoparticles size were revealed between 20
and 50 nm. The antibacterial activity of Ag-NPs tested show a maximum zone of
inhibition of 19 mm for Escherichia coli and Staphylococcus aureus 17 mm for at 125
µg/mL. Green synthesized AgNPs were assessed for antioxidant activity inhibition
rate (DPPH 58.23 % and ABTS 68.87 %). Further, the anticancer activity of AgNPs
exhibited 68.15 % at 100 µg/mL concentration against A549 lung cancer cells.
Additionally, in vitro models using the human red blood cells (HRBC) membrane
stabilisation method (MSM) were used to assess the anti-inflammatory effects of
AgNPs of A. macrobotrys and its shown to have a MSM of 76.6 % at a dosage of 250
µg/mL. A. macrobotrys derived AgNPs possess multi potential activity was used in
future pharmaceutical applications. © 2023 Elsevier B.V.
AN - rayyan-553781399
AU - Sivakumar, S.
AU - Subban, M.
AU - Chinnasamy, R.
AU - Chinnaperumal, K.
AU - Nakouti, I.
AU - El-Sheikh, M. A.
AU - Shaik, J. P.
DO - 10.1016/j.inoche.2023.110787
KW - AgNPs
Andrographis macrobotrys
Antibacterial
Antioxidants
Lung cancer cells (A549)
Lung Neoplasms
Lung
PY - 2023
ST - Green synthesized silver nanoparticles using Andrographis macrobotrys Nees
leaf extract and its potential to antibacterial, antioxidant, anti-inflammatory and
lung cancer cells cytotoxicity effects
T2 - Inorganic Chemistry Communications
TI - Green synthesized silver nanoparticles using Andrographis macrobotrys Nees
leaf extract and its potential to antibacterial, antioxidant, anti-inflammatory and
lung cancer cells cytotoxicity effects
85159218959&doi=10.1016%2fj.inoche.2023.110787&partnerID=40&md5=2ede0775a06c7468973
b177737033b9d
VL - 153
ID - 9340
ER -
TY - JOUR
AB - The emergence of bacteria resistant to antibiotics and the resulting
infections are increasingly becoming a public health issue. Multidrug-resistant
(MDR) bacteria are responsible for infections leading to increased morbidity and
mortality in hospitals, prolonged time of hospitalization, and additional burden to
financial costs. Therefore, there is an urgent need for novel antibacterial agents
that will both treat MDR infections and outsmart the bacterial evolutionary
mechanisms, preventing further resistance development. In this study, a green
synthesis employing nontoxic lignin as both reducing and capping agents was adopted
to formulate stable and biocompatible silver-lignin nanoparticles (NPs) exhibiting
antibacterial activity. The resulting silver-lignin NPs were approximately 20 nm in
diameter and did not agglomerate after one year of storage at 4 degrees C. They
were able to inhibit the growth of a panel of MDR clinical isolates, including
Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa,
Klebsiella pneumoniae, and Acinetobacter baumannii, at concentrations that did not
affect the viability of a monocyte-derived THP-1 human cell line. Furthermore, the
exposure of silver-lignin NPs to the THP-1 cells led to a significant increase in
the secretion of the anti-inflammatory cytokine IL-10, demonstrating the potential
of these particles to act as an antimicrobial and anti-inflammatory agent
simultaneously. P. aeruginosa genes linked with efflux, heavy metal resistance,
capsular biosynthesis, and quorum sensing were investigated for changes in gene
expression upon sublethal exposure to the silver-lignin NPs. Genes encoding for
membrane proteins with an efflux function were upregulated. However, all other
genes were membrane proteins that did not efflux metals and were downregulated.
AN - rayyan-553781401
AU - Slavin, Y. N.
AU - Ivanova, K.
AU - Hoyo, J.
AU - Perelshtein, I.
AU - Owen, G.
AU - Haegert, A.
AU - Lin, Y. Y.
AU - LeBihan, S.
AU - Gedanken, A.
AU - Hafeli, U. O.
AU - Tzanov, T.
AU - Bach, H.
DO - 10.1021/acsami.0c16921
IS - 19
KW - Drug Resistance, Multiple
Bacteria
PY - 2021
SN - 1944-8244 1944-8252
SP - 22098-22109
ST - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
TI - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
VL - 13
Y2 - 5 y3 - 19
ID - 9342
ER -
TY - JOUR
AB - The emergence of bacteria resistant to antibiotics and the resulting
infections are increasingly becoming a public health issue. Multidrug-resistant
(MDR) bacteria are responsible for infections leading to increased morbidity and
mortality in hospitals, prolonged time of hospitalization, and additional burden to
financial costs. Therefore, there is an urgent need for novel antibacterial agents
that will both treat MDR infections and outsmart the bacterial evolutionary
mechanisms, preventing further resistance development. In this study, a green
synthesis employing nontoxic lignin as both reducing and capping agents was adopted
to formulate stable and biocompatible silver-lignin nanoparticles (NPs) exhibiting
antibacterial activity. The resulting silver-lignin NPs were approximately 20 nm in
diameter and did not agglomerate after one year of storage at 4 °C. They were able
to inhibit the growth of a panel of MDR clinical isolates, including Staphylococcus
aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae,
and Acinetobacter baumannii, at concentrations that did not affect the viability of
a monocyte-derived THP-1 human cell line. Furthermore, the exposure of silver-
lignin NPs to the THP-1 cells led to a significant increase in the secretion of the
anti-inflammatory cytokine IL-10, demonstrating the potential of these particles to
act as an antimicrobial and anti-inflammatory agent simultaneously. P. aeruginosa
genes linked with efflux, heavy metal resistance, capsular biosynthesis, and quorum
sensing were investigated for changes in gene expression upon sublethal exposure to
the silver-lignin NPs. Genes encoding for membrane proteins with an efflux function
were upregulated. However, all other genes were membrane proteins that did not
efflux metals and were downregulated.
AN - rayyan-553781848
AU - Slavin, Y. N.
AU - Ivanova, K.
AU - Hoyo, J.
AU - Perelshtein, I.
AU - Owen, G.
AU - Haegert, A.
AU - Lin, Y. Y.
AU - LeBihan, S.
AU - Gedanken, A.
AU - Häfeli, U. O.
AU - Tzanov, T.
AU - Bach, H.
DO - 10.1021/acsami.0c16921
IS - 19
KW - Anti-Bacterial Agents/*pharmacology
Bacteria/drug effects/growth & development
Drug Resistance, Multiple, Bacterial/*drug effects
Humans
Inflammation/prevention & control
Lignin/*chemistry
Silver/*chemistry
THP-1 Cells
Bacteria
LA - eng
N1 - Faculty of Medicine, Division of Infectious Diseases, University of British
Columbia, Vancouver, British Columbia V6T 1Z4, Canada.; Grup de Biotecnologia
Molecular i Industrial, Department of Chemical Engineering, Universitat Politècnica
de Catalunya, Terrassa, Barcelona 08222, Spain.; Grup de Biotecnologia Molecular i
Industrial, Department of Chemical Engineering, Universitat Politècnica de
Catalunya, Terrassa, Barcelona 08222, Spain.; Department of Chemistry, Bar-Ilan
University, Ramat Gan 52900, Israel.; Department of Dentistry, Centre for High-
Throughput Phenogenomics, Faculty of Dentistry, University of British Columbia,
Vancouver, British Columbia V6T 1Z4, Canada.; Laboratory for Advanced Genome
Analysis, Vancouver Prostate Centre, Department of Urologic Sciences, University of
British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.; Laboratory for
Advanced Genome Analysis, Vancouver Prostate Centre, Department of Urologic
Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z4,
Canada.; Laboratory for Advanced Genome Analysis, Vancouver Prostate Centre,
Department of Urologic Sciences, University of British Columbia, Vancouver, British
Columbia V6T 1Z4, Canada.; Department of Chemistry, Bar-Ilan University, Ramat Gan
52900, Israel.; Faculty of Pharmaceutical Sciences, University of British Columbia,
Vancouver, British Columbia V6T 1Z4, Canada.; Grup de Biotecnologia Molecular i
Industrial, Department of Chemical Engineering, Universitat Politècnica de
Catalunya, Terrassa, Barcelona 08222, Spain.; Faculty of Medicine, Division of
Infectious Diseases, University of British Columbia, Vancouver, British Columbia
V6T 1Z4, Canada.
PY - 2021
SP - 22098-22109
ST - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
TI - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
VL - 13
Y2 - 5 y3 - 19
ID - 9774
ER -
TY - JOUR
AB - Purpose: To test the antimicrobial properties, surface topography, reaction
of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant
surfaces containing polysiloxane and nanoscaled silver particles. Materials and
Methods: Implants with polysiloxane coating and nanoscaled silver particles
(Ag/SiOxCy; HyProtect, Bio-Gate) were compared with implants with polysiloxane
coating alone and with noncoated (grit-blasted and acid-etched) implants. A total
of 72 implants were inserted into the calvaria of eight domestic pigs (nine
implants each, three of each type). After 3 months, histologic sections were
evaluated for inflammatory cell infiltration and bone implant contact. Results:
Roughness parameters did not differ between all three implant types. The Ag/SiOxCy
coating exhibited a good antimicrobial effect in vitro and no sign of inflammatory
cell infiltration in vivo. The noncoated implants demonstrated 10.85% and 14.48%
more bone contact than the polysiloxane-coated implants (P =.003) and the
Ag/SiOxCy-coated implants (P =.001), respectively. Osseointegration was not
significantly different between the Ag/SiOxCy-coated and polysiloxane-coated
implants (P =.72). Conclusion: The osseointegration capability of the Ag/SiOxCy-
coated implants was equal to that of the polysiloxane-coated implants but less than
that of the grit-blasted and acid-etched implants. Because of the biocompatibility
of the polysiloxane coating, further studies should be conducted in load-bearing
models and in the oral cavity to investigate the antimicrobial effect of the
embedded silver clusters.
AN - rayyan-553781404
AU - Smeets, R.
AU - Precht, C.
AU - Hahn, M.
AU - Jung, O.
AU - Hartjen, P.
AU - Heiland, M.
AU - Grobe, A.
AU - Holthaus, M. G.
AU - Hanken, H.
DO - 10.11607/jomi.5533
IS - 6
KW - Titanium
Swine
PY - 2017
SN - 0882-2786 1942-4434
SP - 1338-1345
ST - Biocompatibility and Osseointegration of Titanium Implants with a Silver-
Doped Polysiloxane Coating: An In Vivo Pig Model
T2 - INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS
TI - Biocompatibility and Osseointegration of Titanium Implants with a Silver-
VL - 32
Y2 - 11
ID - 9345
ER -
TY - JOUR
AB - PURPOSE: To test the antimicrobial properties, surface topography, reaction
of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant
surfaces containing polysiloxane and nanoscaled silver particles. MATERIALS AND
METHODS: Implants with polysiloxane coating and nanoscaled silver particles
(Ag/SiO(x)C(y); HyProtect, Bio-Gate) were compared with implants with polysiloxane
coating alone and with noncoated (grit-blasted and acid-etched) implants. A total
of 72 implants were inserted into the calvaria of eight domestic pigs (nine
implants each, three of each type). After 3 months, histologic sections were
evaluated for inflammatory cell infiltration and bone implant contact. RESULTS:
Roughness parameters did not differ between all three implant types. The
Ag/SiO(x)C(y) coating exhibited a good antimicrobial effect in vitro and no sign of
inflammatory cell infiltration in vivo. The noncoated implants demonstrated 10.85%
and 14.48% more bone contact than the polysiloxane-coated implants (P = .003) and
the Ag/SiO(x)C(y)-coated implants (P ≤ .001), respectively. Osseointegration was
not significantly different between the Ag/SiO(x)C(y)-coated and polysiloxane-
coated implants (P = .72). CONCLUSION: The osseointegration capability of the
Ag/SiO(x)C(y)-coated implants was equal to that of the polysiloxane-coated implants
but less than that of the grit-blasted and acid-etched implants. Because of the
biocompatibility of the polysiloxane coating, further studies should be conducted
in load-bearing models and in the oral cavity to investigate the antimicrobial
effect of the embedded silver clusters.
AN - rayyan-553781864
AU - Smeets, R.
AU - Precht, C.
AU - Hahn, M.
AU - Jung, O.
AU - Hartjen, P.
AU - Heiland, M.
AU - Gröbe, A.
AU - Holthaus, M. G.
AU - Hanken, H.
DO - 10.11607/jomi.5533
IS - 6
J2 - Int J Oral Maxillofac Implants
KW - Animals
*Coated Materials, Biocompatible
*Dental Implantation, Endosseous
*Dental Implants
Materials Testing
Osseointegration/*physiology
*Siloxanes
*Silver
Surface Properties
Sus scrofa
Swine
*Titanium
Weight-Bearing
Titanium
LA - eng
PY - 2017
SP - 1338–1345
ST - Biocompatibility and Osseointegration of Titanium Implants with a Silver-
T2 - The International journal of oral & maxillofacial implants
TI - Biocompatibility and Osseointegration of Titanium Implants with a Silver-
VL - 32
ID - 9789
ER -
TY - JOUR
AB - The silver ion (Ag(I)) has well established antimicrobial properties and is
widely used in a variety of anti-bacterial ointments and plasters for the control
of wound infections. Wounds are frequently colonised by the bacterium
Staphylococcus aureus and the aim of the work presented here was to establish how
S. aureus responded following exposure to Ag(I). Exposure of S. aureus to Ag(I)
resulted in the release of a range of proteins from cells. Analysis of proteins
released revealed a number of proteins associated with the stress response (e.g.
alkaline shock protein, methionine sulfoxide reductase), virulence (e.g. signal
transduction protein) and metabolism (e.g. lipase, acetate kinase, phosphoglycerate
mutase). The release of toxins (e.g. α-hemolysin, bifunctional autolysin,
leucocidin F) was decreased. These results indicated that, while silver is a potent
antimicrobial agent, exposure of S. aureus to this metal results in the release of
a variety of proteins from the cell. Many of the proteins showing increased release
were antigenic and would have the potential to induce an inflammatory response at
the site of infection and thus delay healing.
AN - rayyan-553782359
AU - Smith, A.
AU - McCann, M.
AU - Kavanagh, K.
DO - 10.1016/j.tiv.2013.04.007
IS - 6
KW - Anti-Bacterial Agents/*toxicity
Bacterial Proteins/*metabolism
Proteomics
Silver/*toxicity
Staphylococcus aureus/*drug effects/metabolism
Staphylococcus
Proteome
LA - eng
N1 - Department of Biology, National University of Ireland Maynooth, Co. Kildare,
Ireland.
PY - 2013
SP - 1644-8
ST - Proteomic analysis of the proteins released from Staphylococcus aureus
following exposure to Ag(I)
BIBRA
TI - Proteomic analysis of the proteins released from Staphylococcus aureus
following exposure to Ag(I)
VL - 27
Y2 - 9
ID - 10266
ER -
TY - JOUR
AB - Deep burn injuries are complicated traumas accompanying severe oxidative
stress and impairment of inherent cellular defense mechanisms against external
bacteria. Accordingly, it is required to suppress oxidative stress and support the
recovery of intrinsic defense systems in burn wound lesions, but limited topical
treatments are clinically available. Herein, antioxidative and anti-inflammatory
2H-WS2 nanosheets are developed as a biocompatible, topical antipyrotic for the
treatment of deep burn wounds. The 2H-WS2 nanosheets functionalized with a block
copolymer can effectively suppress extrinsic apoptosis, lipid peroxidation, and
inflammation in human keratinocytes by scavenging intracellular reactive oxygen
species (ROS) and reactive nitrogen species (RNS). Moreover, the 2H-WS2 nanosheets
markedly stimulate the up-regulation of innate antioxidant enzymes and
antimicrobial peptides in the skin cells, enhancing their intrinsic cellular
defense systems. The combined effects of this 2H-WS2 antipyrotic enables a higher
efficacy in the deep burn wounds of mice, accelerating re-epithelialization and
satisfactory wound healing as compared with commercial silver sulfadiazine (SSD).
The therapeutic mechanism of the 2H-WS2 nanosheets for deep burn wounds is proposed
herein and found to be distinctly different from that of SSD. The nanotherapeutics
of multifunctional 2H-WS2 nanosheets show potential for use in the treatment of
other oxidative stress-induced and inflammatory diseases. © 2022 Elsevier Ltd
AN - rayyan-553781406
AU - So, Y.
AU - Yim, D.
AU - Son, W.
AU - Lee, H.
AU - Lee, S.
AU - Choi, C.
AU - Yang, C. S.
AU - Kim, J. H.
DO - 10.1016/j.apmt.2022.101591
KW - Anti-apoptosis
Anti-inflammation
Burn wound healing
Oxidative stress suppression
WS2 nanosheet antipyrotic
Antioxidants
Biocompatibility
Block copolymers
Cell death
Diseases
Mammals
Network security
Oxidative stress
Pathology
Tungsten compounds
Burn injury
Cellulars
Deep burn
Defence systems
Wound healing
WS2 nanosheet antipyrotic
Nanosheets
Burns
PY - 2022
ST - Deciphering the therapeutic mechanism of topical WS2 nanosheets for the
effective therapy of burn injuries
T2 - Applied Materials Today
TI - Deciphering the therapeutic mechanism of topical WS2 nanosheets for the
effective therapy of burn injuries
85134852699&doi=10.1016%2fj.apmt.2022.101591&partnerID=40&md5=97309a945a66a8245513c
84b7bd8621a
VL - 29
ID - 9347
ER -
TY - JOUR
AB - Background: Wound healing is a complicated process involving the
proliferation of the epithelial cells, deposition of granulation tissue as well as
recruitment of inflammatory cells. It also is a hot topic of research for trauma,
orthopedics and general surgery studies. There are many forms of cells involved in
this process. This study aimed to design a tissue-engineered wound dressing
consisting of chitosan fibers containing silver ion bioactive nanoparticles for
wound healing. Methods: The present study is an experimental study that was
conducted in the research laboratory of the Department of Biology of Mohaghegh
Ardabili University from April to November 2019. All experiments of this study have
been performed under the ethical guideline of Helsinki and in accordance with the
Ethics Committee of the Mohaghegh Ardabili University of Ardabil (Iran). The wound
dressing of nanofibers was prepared by the sol-gel method. Cytotoxicity was
assessed by MTT assay. Then the antimicrobial properties of nanofibers were
determined by the disk diffusion method. SEM and AFM images were obtained from
nanofibers. Finally, nanofibers were analyzed by the FTRI method. Results: Results
of the prepared tissue-engineered wound dressing consisting of chitosan fibers
containing silver ion-doped bioactive nanoparticles showed that cytotoxicity was at
an appropriate level. The nanofibers prepared with 2% silver nanoparticles produced
a 10 mm inhibition zone against Staphylococcus aureus and a 9 mm inhibition zone
against Escherichia coli. Therefore, the best percentage of scaffolds in the
present study was 2%. Also, results of the SEM micrographs and AFM image analysis
of the scaffolds showed that the nanofibers had good roughness and a proper
structure for cell seeding and attachments. Besides that, FTIR analysis also showed
that the prepared nanofibers had standard bonds. Conclusion: Chitosan-Silver
nanoparticles scaffold have antimicrobial activity on Gram-negative and positive
bacteria. The results of the toxicity test also showed that it did not have much
toxicity on the cultured cells. Therefore, it can be considered for therapeutic
applications, such as wound dressing. © 2021 Tehran University of Medical Sciences.
AN - rayyan-553781407
AU - Sohi, M. M.
AU - Asadi, A.
AU - Milan, P. B.
AU - Sharifi, E.
AU - Abdolmaleki, A.
IS - 4
KW - chitosan
nanoparticles
regeneration
tissue engineering
nanofiber
silver nanoparticle
Article
cell adhesion
cytotoxicity
disk diffusion
Escherichia coli
human
human tissue
MTT assay
sol-gel
tumor seeding
wound healing
zone of inhibition
Wound Healing
PY - 2021
SP - 290-298
ST - Preparation of tissue-engineered wound dressing consisting of chitosan fibers
containing silver ion-doped bioactive nanoparticles for wound healing
T2 - Tehran University Medical Journal
TI - Preparation of tissue-engineered wound dressing consisting of chitosan fibers
containing silver ion-doped bioactive nanoparticles for wound healing
85110447721&partnerID=40&md5=23b329e78dc778d13ead63ac7202848b
VL - 79
ID - 9348
ER -
TY - JOUR
AB - The body's normal immune response against any invading pathogen that causes
infection in the body results in inflammation. The sudden transformation in
inflammation leads to the rise of inflammatory diseases such as chronic
inflammatory bowel disease, autoimmune disorders, and colorectal cancer (different
types of cancer develop at the site of chronic infection and inflammation).
Inflammation results in two ways: short-term inflammation i.e., non-specific,
involves the action of various immune cells; the other results in long-term
reactions lasting for months or years. It is specific and causes angiogenesis,
fibrosis, tissue destruction, and cancer progression at the site of inflammation.
Cancer progression relies on the interaction between the host microenvironment and
tumor cells along with the inflammatory responses, fibroblast, and vascular cells.
The two pathways that have been identified connecting inflammation and cancer are
the extrinsic and intrinsic pathways. Both have their own specific role in linking
inflammation to cancer, involving various transcription factors such as Nuclear
factor kappa B, Activator of transcription, Single transducer, and Hypoxia-
inducible factor, which in turn regulates the inflammatory responses via Soluble
mediators cytokines (such as Interleukin-6, Hematopoietin-1/Erythropoietin, and
tumor necrosis factor), chemokines (such as Cyclooxygenase-2, C-X-C Motif
chemokines ligand-8, and IL-8), inflammatory cells, cellular components (such as
suppressor cells derived from myeloid, tumor-associated macrophage, and
acidophils), and promotes tumorigenesis. The treatment of these chronic
inflammatory diseases is challenging and needs early detection and diagnosis.
Nanotechnology is a booming field nowadays for its rapid action and easy
penetration inside the infected destined cells. Nanoparticles are widely classified
into different categories based on their different factors and properties such as
size, shape, cytotoxicity, and others. Nanoparticles emerged as excellent with
highly progressive medical inventions to cure diseases such as cancer, inflammatory
diseases, and others. Nanoparticles have shown higher binding capacity with the
biomolecules in inflammation reduction and lowers the oxidative stress inside
tissue/cells. In this review, we have overall discussed inflammatory pathways that
link inflammation to cancer, major inflammatory diseases, and the potent action of
nanoparticles in chronic inflammation-related diseases.
AN - rayyan-553781408
AU - Sohrab, S. S.
AU - Raj, R.
AU - Nagar, A.
AU - Hawthorne, S.
AU - Paiva-Santos, A. C.
AU - Kamal, M. A.
AU - El-Daly, M. M.
AU - Azhar, E. I.
AU - Sharma, A.
DO - 10.3390/molecules28114413
IS - 11
KW - Inflammation
PY - 2023
SN - 1420-3049
ST - Chronic Inflammation's Transformation to Cancer: A Nanotherapeutic Paradigm
T2 - MOLECULES
TI - Chronic Inflammation's Transformation to Cancer: A Nanotherapeutic Paradigm
VL - 28
Y2 - 5 y3 - 29
ID - 9349
ER -
TY - JOUR
AB - Moringa oleifera and Azadirachta indica (Neem) are trees with nutritional,
pharmacological values, and various preparations from almost all parts of the plant
especially from leaves are used in folk medicine for many infirmity treatments.
This study aimed to investigate the polyphenolic burden, in vitro antioxidant, and
anti-inflammatory properties of M. oleifera, Neem leaves crude aqueous extracts and
synthesized Ag-NPs. Moringa oleifera and Neem leaves were extracted using de-
ionized water. The extracts were screened for phenolic compounds by HPLC analysis.
Ag-NPs were obtained by a modified method, in the presence of NaBH4 at its half
usual concentration, and they were characterized by UV-VIS, Zeta potential &
particle size, and TEM respectively. The antioxidant activity was determined by
DPPH, ABTS, and FRAP assays, and the anti-inflammatory activity was determined
against macrophage cell line RAW 264.7 by Nitric Oxide assay. The results indicated
that synthesized Ag-NPs from Moringa and Neem show enhanced antioxidant with (75.47
+/- 0.16%) and (65.97 +/- 0.17%) at 400 mu g/mL according to DPPH assay, ABTS assay
with (82.93 +/- 0.63%) and (78.76 +/- 0.34%) at 200 mu g/mL, and FRAP assay with
(113.67 +/- 0.68 mu M) and (105.31 +/- 0.33 mu M) at 400 mu g/mL, respectively.
After the LPS treatment, the anti-inflammatory potential of different tested
samples showed (67.95 +/- 0.85%) and (58.29 +/- 0.90%) inhibition at 100 mu g/mL
for Ag-NPs from Moringa and Neem, compared to Moringa and Neem crude aqueous
extracts with (30.60 +/- 0.43%) and (26.25 +/- 1.28%) at 100 mu g/mL respectively.
Synthesized Ag-NPs and crude aqueous extracts from Moringa and Neem may be
effectively used to possess important antioxidant and anti-inflammatory properties.
AN - rayyan-553781409
AU - Solaiman, M. A.
AU - Ali, M. A.
AU - Abdel-Moein, N. M.
AU - Mahmoud, E. A.
DO - 10.1016/j.bcab.2020.101832
KW - Macrophages
Aged
Aging
Antioxidants
PY - 2020
SN - 1878-8181
ST - Synthesis of Ag-NPs developed by green-chemically method and evaluation of
antioxidant activities and anti-inflammatory of synthesized nanoparticles against
LPS-induced NO in RAW 264.7 macrophages
T2 - BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
TI - Synthesis of Ag-NPs developed by green-chemically method and evaluation of
antioxidant activities and anti-inflammatory of synthesized nanoparticles against
LPS-induced NO in RAW 264.7 macrophages
VL - 29
Y2 - 10
ID - 9350
ER -
TY - JOUR
AB - Introduction The success of endodontic surgery depends on the
histocompatibility of the root-end filling material. Applications of nanotechnology
improve their performance. Aim of the work Aim of the work was to compare the
effect of a mineral trioxide aggregate (MTA) and a bioceramic nanoparticulate
bioaggregate (BNB) on the histological structure of draining axillary lymph nodes
of adult male albino rats after their surgical implantation into the skin.
Materials and methods Forty adult male albino rats were divided into control and
experimental groups. The latter was subdivided into MTA and BNB surgically
implanted subgroups. After 7 days, the rats were sacrificed. Paraffin sections from
both the proximal part of the dorsal skin and draining axillary lymph nodes were
processed for HandE staining. Lymph node sections were further subjected to silver
reticulin, Verhoeff's Van Gieson stains as well as kappa light chains. Quantitative
assessments and statistical analysis of the results were carried out. Results There
was an increase in mononuclear inflammatory cells infiltrating the skin in the MTA
subgroup. Lymph nodes of the MTA subgroup showed a marked decrease in the
lymphocyte content of lymphatic nodules, wide lymph sinuses, multinucleate giant
cells, and many macrophages. In the BNB-treated subgroup, lymphatic nodules had
wide corona and small germinal centers. Reticular and collagen fibers were
increased in the MTA subgroup. Kappa light chains' immunoreactions were strong
positive in MTA and mild positive in BNB subgroups. A highly significant increase
in the mean area% of all fibers and kappa light chain immunoexpression of lymph
nodes of the MTA subgroup were observed. Conclusion and recommendation MTA had less
biocompatibility. BNB showed limited signs of acute inflammation. BNB is an up-to-
date alternative to the currently used root-end filling materials. The chronic
effects caused by BNB may require further study. © 2012 The Egyptian Journal of
Histology.
AN - rayyan-553781410
AU - Soliman, H. M.
AU - Anwar, R. I.
DO - 10.1097/01.EHX.0000420214.04776.64
IS - 4
KW - Bioceramic Nanoparticulate Bioaggregate
Collagen Fibers
Lymph Node
Mineral Trioxide Aggregate
Root-End Filling Materials
Skin
Rats
Atrioventricular Node
PY - 2012
SP - 736-748
ST - Effect of surgically implanted root-end filling materials on the structure of
draining lymph nodes of male albino rats: Histological and immunohistochemical
study
TI - Effect of surgically implanted root-end filling materials on the structure of
draining lymph nodes of male albino rats: Histological and immunohistochemical
study
84878078639&doi=10.1097%2f01.EHX.0000420214.04776.64&partnerID=40&md5=730fc06ed5919
c7f3b4d36eedf32909f
VL - 35
ID - 9351
ER -
TY - JOUR
AB - Dietary supplementation with n-3 polyunsaturated fatty acids (n-3 PUFA) has
been used as an adjunct therapy for psoriasis due to its anti-inflammatory
properties. Free fatty acid receptor 4 (FFA4 or GPR120) is a receptor-sensing n-3
PUFA. In the present study, we examined whether FFA4 acted as a therapeutic target
for n-3 PUFA in psoriasis therapy. Experimentally, psoriasis-like skin lesions were
induced by treatment with imiquimod for 6 consecutive days. A selective FFA4
agonist, Compound A (30 mg/kg), was used in FFA4 WT and FFA4 KO mice. Imiquimod-
induced psoriasis-like skin lesions, which present as erythematous papules and
plaques with silver scaling, as well as markedly elevated IL-17/IL-23 cytokine
levels in skin tissues, were significantly suppressed by Compound A in FFA4 WT
mice, but not in FFA4 KO mice. Enlarged lymph nodes and spleens, as well as
imiquimod-induced, elevated IL-17/IL-23 cytokine levels, were also strongly
suppressed by Compound A in FFA4 WT mice, but not in FFA4 KO mice. Imiquimod-
induced increases in the CD4(+)IL-17A(+) T cell population in lymph nodes and
spleens were suppressed by Compound A treatment in FFA4 WT mice; however, this was
not seen in FFA4 KO mice. Furthermore, compound A suppressed the differentiation of
CD4(+) naïve T cells from splenocytes into T(H)17 cells in an FFA4-dependent
manner. In conclusion, we demonstrated that the activation of FFA4 ameliorates
imiquimod-induced psoriasis, and the suppression of the differentiation of T(H)17
cells may partly contribute to its efficacy. Therefore, we suggest that FFA4 could
be a therapeutic target for psoriasis therapy.
AN - rayyan-553782343
AU - Son, S. E.
AU - Koh, J. M.
AU - Im, D. S.
DO - 10.3390/ijms23094482
IS - 9
J2 - Int J Mol Sci
KW - Animals
Cytokines/therapeutic use
Fatty Acids, Nonesterified/therapeutic use
*Fatty Acids, Omega-3/therapeutic use
Imiquimod/toxicity
Interleukin-17/genetics
Interleukin-23
Mice
*Psoriasis/chemically induced/drug therapy/pathology
Skin/pathology
Fatty Acids, Nonesterified
Psoriasis
LA - eng
N1 - Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung
Hee University, Seoul 02447, Korea.; Division of Endocrinology and Metabolism, Asan
Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.;
Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee
University, Seoul 02447, Korea.; Department of Basic Pharmaceutical Sciences,
Graduate School, Kyung Hee University, Seoul 02447, Korea.
PY - 2022
ST - Free Fatty Acid Receptor 4 (FFA4) Activation Ameliorates Imiquimod-Induced
Psoriasis in Mice
TI - Free Fatty Acid Receptor 4 (FFA4) Activation Ameliorates Imiquimod-Induced
Psoriasis in Mice
VL - 23
Y2 - 4 y3 - 19
ID - 10251
ER -
TY - JOUR
AB - With the rapid development of nanotechnology, metallic (metal or metal oxide)
nanoparticles (NPs) are widely used in many fields such as cosmetics, the food and
building industries, and bio-medical instruments. Widespread applications of
metallic NP-based products increase the health risk associated with human
exposures. Studies revealed that the brain, a critical organ that consumes
substantial amounts of oxygen, is a primary target of metallic NPs once they are
absorbed into the body. Oxidative stress (OS), apoptosis, and the inflammatory
response are believed to be the main mechanisms underlying the neurotoxicity of
metallic NPs. Other studies have disclosed that antioxidant pretreatment or co-
treatment can reverse the neurotoxicity of metallic NPs by decreasing the level of
reactive oxygen species, up-regulating the activities of antioxidant enzymes,
decreasing the proportion of apoptotic cells, and suppressing the inflammatory
response. These findings suggest that the neurotoxicity of metallic NPs might
involve a cascade of events following NP-induced OS. However, additional research
is needed to determine whether NP-induced OS plays a central role in the
neurotoxicity of metallic NPs, to develop a comprehensive understanding of the
correlations among neurotoxic mechanisms and to improve the bio-safety of metallic
NP-based products.
AN - rayyan-553781413
AU - Song, B.
AU - Zhang, Y. L.
AU - Liu, J.
AU - Feng, X. L.
AU - Zhou, T.
AU - Shao, L. Q.
DO - 10.1186/s11671-016-1508-4
PY - 2016
SN - 1931-7573 1556-276X
ST - Is Neurotoxicity of Metallic Nanoparticles the Cascades of Oxidative Stress?
T2 - NANOSCALE RESEARCH LETTERS
TI - Is Neurotoxicity of Metallic Nanoparticles the Cascades of Oxidative Stress?
VL - 11
Y2 - 6 y3 - 13
ID - 9354
ER -
TY - JOUR
AB - Titanium dioxide nanoparticles (TiO2 NPs) possess unique characteristics and
are widely used in many fields. Numerous in vivo studies, exposing experimental
animals to these NPs through systematic administration, have suggested that TiO2
NPs can accumulate in the brain and induce brain dysfunction. Nevertheless, the
exact mechanisms underlying the neurotoxicity of TiO2 NPs remain unclear. However,
we have concluded from previous studies that these mechanisms mainly consist of
oxidative stress (OS), apoptosis, inflammatory response, genotoxicity, and direct
impairment of cell components. Meanwhile, other factors such as disturbed
distributions of trace elements, disrupted signaling pathways, dysregulated
neurotransmitters and synaptic plasticity have also been shown to contribute to
neurotoxicity of TiO2 NPs. Recently, studies on autophagy and DNA methylation have
shed some light on possible mechanisms of nanotoxicity. Therefore, we offer a new
perspective that autophagy and DNA methylation could contribute to neurotoxicity of
TiO2 NPs. Undoubtedly, more studies are needed to test this idea in the future. In
short, to fully understand the health threats posed by TiO2 NPs and to improve the
bio-safety of TiO2 NPs-based products, the neurotoxicity of TiO2 NPs must be
investigated comprehensively through studying every possible molecular mechanism.
AN - rayyan-553781414
AU - Song, B.
AU - Zhang, Y. L.
AU - Liu, J.
AU - Feng, X. L.
AU - Zhou, T.
AU - Shao, L. Q.
DO - 10.3762/bjnano.7.57
KW - Titanium
PY - 2016
SN - 2190-4286
ST - Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on
molecular mechanisms
T2 - BEILSTEIN JOURNAL OF NANOTECHNOLOGY
TI - Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on
molecular mechanisms
VL - 7
Y2 - 4 y3 - 29
ID - 9355
ER -
TY - JOUR
AB - (1) Background: Infections of pathogenic microorganisms can be life-
threatening due to delayed healing or even worsening conditions in tissue
engineering and regenerative medicine. The excessive presence of reactive oxygen
species in damaged and infected tissues causes a negative inflammatory response,
resulting in failed healing. Thus, the development of hydrogels with antibacterial
and antioxidant abilities for the treatment of infectious tissues is in high
demand. (2) Methods: We herein describe the development of green-synthesized
silver-composited polydopamine nanoparticles (AgNPs), which are fabricated by the
self-assembly of dopamine as a reducing and antioxidant agent in the presence of
silver ions. (3) Results: The facile and green-synthesized AgNPs have a nanoscale
diameter with mostly spherical shapes, with various shapes coexisting. The
particles are stable in an aqueous solution for up to 4 weeks. In addition,
remarkable antibacterial activity against Gram-positive and -negative bacterial
strains and antioxidant capabilities were evaluated by in vitro assays. When
incorporated into biomaterial hydrogels at concentrations above 2 mg L(-1), the
hydrogels produced powerful antibacterial effects. (4) Conclusions: This study
describes a biocompatible hydrogel with antibacterial and antioxidant activities
from the introduction of facile and green-synthesized AgNPs as a safer tool for the
treatment of damaged tissues.
AN - rayyan-553781794
AU - Song, G. J.
AU - Choi, Y. S.
AU - Hwang, H. S.
AU - Lee, C. S.
DO - 10.3390/gels9030183
IS - 3
J2 - Gels
KW - Antioxidants
Hydrogel
Hydrogels
LA - eng
N1 - Department of Pharmaceutical Engineering and Biotechnology, Sun Moon
University, Asan 31460, Republic of Korea.; Department of Pharmaceutical Science,
University of California, Irvine, CA 92697, USA.; Department of Pharmaceutical
Engineering, Dankook University, Cheonan 31116, Republic of Korea.; Department of
Pharmaceutical Engineering and Biotechnology, Sun Moon University, Asan 31460,
Republic of Korea.
PY - 2023
ST - Silver-Composited Polydopamine Nanoparticles: Antibacterial and Antioxidant
Potential in Nanocomposite Hydrogels
T2 - Gels (Basel, Switzerland)
TI - Silver-Composited Polydopamine Nanoparticles: Antibacterial and Antioxidant
Potential in Nanocomposite Hydrogels
VL - 9
Y2 - 2 y3 - 27
ID - 9724
ER -
TY - JOUR
AB - Nanotechnology is a promising technology of the twenty-first century, being a
rapidly evolving field of research and industrial innovation widely applied in our
everyday life. Silver nanoparticles (AgNP) are considered the most commercialized
nanosystems worldwide, being applied in diverse sectors, from medicine to the food
industry. Considering their unique physical, chemical and biological properties,
AgNP have gained access into our daily life, with an exponential use in food
industry, leading to an increased inevitable human oral exposure. With the growing
use of AgNP, several concerns have been raised, in recent years, about their
potential hazards to human health, more precisely their pro-inflammatory effects
within the gastrointestinal system. Therefore a review of the literature has been
undertaken to understand the pro-inflammatory potential of AgNP, after human oral
exposure, in the intestine. Despite the paucity of information reported in the
literature about this issue, existing studies indicate that AgNP exert a pro-
inflammatory action, through generation of oxidative stress, accompanied by
mitochondrial dysfunction, interference with transcription factors and production
of cytokines. However, further studies are needed to elucidate the mechanistic
pathways and molecular targets involved in the intestinal pro-inflammatory effects
of AgNP.
AN - rayyan-553781415
AU - Sousa, A.
AU - Bradshaw, T. D.
AU - Ribeiro, D.
AU - Fernandes, E.
AU - Freitas, M.
DO - 10.1007/s00204-022-03270-w
IS - 6
KW - Intestines
PY - 2022
SN - 0340-5761 1432-0738
SP - 1551-1571
ST - Pro-inflammatory effects of silver nanoparticles in the intestine
T2 - ARCHIVES OF TOXICOLOGY
TI - Pro-inflammatory effects of silver nanoparticles in the intestine
VL - 96
Y2 - 6
ID - 9356
ER -
TY - JOUR
AB - BACKGROUND: There are no records of comparative studies on the immunological,
histological and immunohistochemical aspects of vitiligo, halo nevus and vitiligoid
variant of lupus erythematosus in the literature. The studies available present
only descriptive clinical data on leucoderma that accompanies lupus erythematosus
in its diverse clinical forms. OBJECTIVES: 1- To evaluate the immunohistochemical
differences between vitiligo, halo nevus and vitiligoid variant of lupus
erythematosus; 2- To verify whether the depigmentation observed in the diverse
clinical forms of lupus is due to post-inflammatory destruction or to specific
immunological attack on melanocytes. METHODS: 1- Detection of melanocyte
antibodies: by direct and indirect immunofluorescence on nevus and melanoma cells;
2- Cytotoxicity evaluation: study of the activity of NK cells against cultivated
melanoma cells; 3- Histopathological study of melanocytes and melanin:
histopathology with hematoxylin-eosin, Fontana- Masson, Dopa and Dopa + silver and
S-100 protein test by immunoperoxidase. RESULTS: Vitiligo and halo nevus patients
presented to antimelanocyte antibodies in 25% of cases. Patients with vitiligoid
variant of lupus erythematous also presented these antibodies. The presence of risk
factors favoring cellular cytotoxicity was demonstrated in vitiligo and/or halo
nevus, as well as in the vitiligoid variant of lupus erythematous. Staining with
Dopa + silver nitrate was superior to traditional staining and to S-100 protein to
detect melanocytes and/or melanin in depigmented lesions of vitiligo and/or halo
nevus and vitiligoid variant of lupus erythematous. CONCLUSION: The results confirm
the existence of antimelanocyte antibodies in vitiligo and halo nevus. It is not
possible to rule out some immunological phenomena similar to those occurring in
vitiligo and halo nevus in the genesis of vitiligoid lesions in lupus erythematous.
The detection of melanocytes in achromic lesions of vitiligo suggests the
predominance of a functional inhibitory mechanism rather than cell destruction in
the genesis of the disease. ©2005 by Anais Brasileiros de Dermatologia.
AN - rayyan-553781418
AU - Souza Filho, L. G. C.
AU - Rivitti, E. A.
AU - Miyauchi, L. M.
AU - Sotto, M. N.
AU - Maria, D. A.
AU - Puejo, S. S. T.
AU - Alves, V. A. F.
DO - 10.1590/s0365-05962005000200004
IS - 2
KW - Allergy and immunology
Lupus
Nevus, pigmented
Vitiligo
cell antibody
DOPA
eosin
hematoxylin
melanin
melanocyte antibody
peroxidase
protein S 100
silver
unclassified drug
antibody detection
article
clinical feature
comparative study
cytotoxicity
depigmentation
halo nevus
histopathology
human
immunofluorescence
immunopathology
lupus erythematosus
melanocyte
natural killer cell
nevus
risk factor
vitiligo
vitiligoid variant of lupus erythematosus
PY - 2005
SP - 143-148
ST - Comparative study of vitiligo, halo nevus, and vitiligoid variant of lupus
erythematosus by immunological, histological, and immunohistochemical methods
T2 - Anais Brasileiros de Dermatologia
TI - Comparative study of vitiligo, halo nevus, and vitiligoid variant of lupus
erythematosus by immunological, histological, and immunohistochemical methods
20344391390&doi=10.1590%2fs0365-
05962005000200004&partnerID=40&md5=2292786e1cfa79bb11eff78de40e1550
VL - 80
ID - 9359
ER -
TY - GEN
AB - O objetivo deste estudo foi avaliar a atividade antimicrobiana/antibiofilme e
a viabilidade celular de nanopartículas de prata (NPsAg) obtidas por uma síntese
'green' associadas ou não ao ß-glicerofosfato de cálcio (GPCa) contra Streptococcus
mutans e espécies de Candida (cepas de referência e isolados clínicos orais
incluindo cepas resistentes ao fluconazol). O efeito destes nanocompostos em
combinação com o tirosol (TIR), fluconazol (FLC), nistatina (NIT) e anfotericina B
(AnB) também foi avaliado. Além disso, nós avaliamos comparativamente as alterações
do transcriptoma de células de C. glabrata CBS138 após exposição às NPsAg e ao Íon
prata (Ag+ ). Inicialmente, as NPsAg foram sintetizadas por meio da redução do
nitrato de prata com extratos de diferentes partes (casca, folha e semente) de uma
romã (Punica granatum L.) associadas ou não ao GPCa. A Concentração Inibitória
Mínima (CIM) e as Concentrações Fungicida/Bactericida Mínima (CFM e CBM) dos
nanocompostos (NPsAg e NPsAgGPCa) associados ou não ao TIR contra S. mutans e C.
albicans foram determinadas pelo método de microdiluição. E, a ação das NPsAg
combinadas com FLC, NIT e AnB também foi avaliada em condição planctônica contra
isolados clínicos orais de espécies de Candida. O efeito dos nanocompostos
associados ou não ao TIR sobre a viabilidade celular de fibroblastos da linhagem
L929 e a produção de citocinas foi avaliado através dos ensaios de MTT e ELISA,
respectivamente. O número de hifas foi quantificado em biofilmes de C. albicans
formados na presença das NPsAg com ou sem soro fetal bovino (SFB). O efeito das
NPsAg em inibir a formação do biofilme (em C. albicans e C. glabrata) também foi
investigado através do ensaio de PrestoBlue e por meio da microscopia eletrônica de
varredura. Os nanocompostos (NPsAg e NPsAg-GPCa) associados ou não ao TIR foram
aplicados sobre biofilmes de S. mutans e de espécies de Candida (12 e 24 h) e, após
24 h de contato, sua atividade antibiofilme foi determinada por meio da enumeração
das unidades formadoras de colônias (UFCs) e ensaio de PrestoBlue. Além disso, uma
análise transcriptômica foi realizada utilizando microchips de DNA ('microarrays')
a fim de avaliar quais genes estão mais ou menos expressos como resposta às NPsAg
(no estado planctônico e formando biofilmes) e ao Íon Ag+ (formando biofilmes). As
soluções antimicrobianas sintetizadas neste estudo (NPsAg e NPsAgGPCa) apresentaram
atividade antimicrobiana contra os microrganismos testados. Além disso, menores
valores de CIM foram obtidos quando estes nanocompostos foram associados ao TIR,
FLC, NIT e AnB apresentando um efeito sinérgico. NPsAg e NPsAgGPCa não foram
tóxicas às células L929, aumentaram a produção de fator de crescimento celular e
não promoveram alterações significativas na liberação de Interleucina-6. Os
nacompostos associados ao TIR não apresentaram efeito citotóxico sobre culturas de
L929, exceto para a maior concentração (NPsAg 39,05 µg/mL + TIR 1,25 mM). A
presença das NPsAg reduziu drasticamente o número de hifas em biofilmes de C.
albicans na presença ou na ausência de SFB. A quantidade de biofilme das espécies
de Candida formado na presença das NPsAg reduziu para mais de 50%. Após 24 h de
tratamento com os nanocompostos (NPsAg e NPsAg-GPCa) em biofilmes de S. mutans,
houve uma redução significativa no número de UFCs sendo similar à clorexidina. Uma
redução na viabilidade de biofilmes de C. glabrata também foi observada após
exposição às NPsAg (24 h). Entretanto, os nanocompostos (NPsAg e NPsAg-GPCa)
associados ou não ao TIR não foram efetivos contra biofilmes de C. albicans. Após
exposição às NPsAg e ao Íon Ag+ , alterações no transcriptoma de células de C.
glabrata CBS138 foram observadas: no estado planctônico, houve uma superexpressão
dos genes responsáveis pela biossíntese de metionina e de lisina e uma subexpressão
dos genes relacionados com o transporte transmembrana; e, as células expostas às
NPsAg responder m de forma distinta em relação ao Íon Ag+ , indicando que as NPsAg
podem apresentar um mecanismo de ação diferente. Estes achados podem auxiliar nas
decisões terapêuticas com formulações contendo NPsAg ou NPsAg-GPCa associadas ou
não a diferentes drogas em pacientes com cárie dentária e candidíase oral(AU) The
aim of this study was to evaluate the antimicrobial/antibiofilm activities and the
cell viability of silver nanoparticles (AgNPs) obtained by a 'green' synthesis
associated or not to ß-calcium glycerophosphate (CaGP) against Streptococcus mutans
and Candida species (reference strains and oral clinical isolates including azole-
resistant strains). The effect of these nanocompounds in combination with tyrosol
(TYR), fluconazole (FLC), nystatin (NYT) and amphotericin B (AmB) also was
evaluated. Furthermore, we evaluated, comparatively, the transcriptome alterations
of cells of C. glabrata CBS138 after exposition to AgNPs and to silver ion (Ag+ ).
Initially, AgNPs were synthesized by reducing silver nitrate with extracts of
different parts (peel, leaves and seeds) of a pomegranate (Punica granatum L.)
associated or not to CaGP. Minimum Inhibitory Concentration (MIC) and Minimum
Fungicidal/Bactericidal Concentrations (MFC and MBC) of the nanocomposites (AgNPs
and AgNPs-CaGP) associated or not to TYR against S. mutans and C. albicans were
determined by the microdilution method. And, the action of the AgNPs combined with
FLC, NYT and AmB also was evaluated in planktonic condition against oral clinical
isolates of Candida species. The effect of the nanocomposites associated or not to
TYR on cell viability of fibroblasts (L929) and cytokines production was evaluated
through MTT and ELISA assays, respectively. The number of cells undergoing
filamentation was quantified in C. albicans biofilms formed in the presence of
AgNPs with or without fetal bovine serum (FBS). The effect of AgNPs in inhibit the
formation of biofilm (in C. albicans and C. glabrata) also was investigated through
PrestoBlue assay and scanning electron microscope. Biofilms of S. mutans and
Candida species (12 and 24 h) were treated with nanocomposites (AgNPs and
AgNPsCaGP) associated or not to TYR for 24 h and, then, the viability of these
biofilms was determined through PrestoBlue assay and colony forming units (CFUs).
Moreover, a transcriptome analysis was performed using microarrays to determine
which genes are up- or down-regulated as response to AgNPs (in the planktonic state
and forming biofilms) and to Ag+ ion (forming biofilms). Antimicrobial solutions
synthesized in this study (AgNPs and AgNPs-CaGP) presented antimicrobial activity
against tested microorganisms. Furthermore, lower values of MIC were obtained when
these nanocompounds were associated to TYR, FLC, NYT and AmB showing a synergistic
effect. AgNPs and AgNPs-CaGP were not toxic to L929 cells, increased the stem cell
factor production and did not promote significant alterations in the Interleukine-6
release. The nanocomposites associated to TYR did not present cytotoxic effect on
L929 cultures, except for the higher concentration (AgNPs 39.05 µg/mL + TYR 1.25
mM). The incubation in the presence of the AgNPs drastically reduced the number of
cells exhibiting hyphae, this effect being observed either in the presence or
absence of FBS. The amount of biofilm formed by Candida species in the presence of
the AgNPs was reduced by more than 50% the one formed in the absence of the
nanoparticles, this reduction being further increased to more than 90% when the
concentration of the AgNPs was increased. After 24 h of treatment with the
nanocompounds (AgNPs and AgNPs-CaGP) in S. mutans biofilms, there was a significant
reduction in the number of CFUs being similar to chlorhexidine. A reduction in the
viability of C. glabrata biofilms also was observed after exposition to AgNPs (24
h). However, the nanocomposites (AgNPs and AgNPs-CaGP) associated or not to TYR
were not effective against C. albicans biofilms. After exposition to AgNPs and to
Ag+ ion, alterations in the transcript me of cells of C. glabrata CBS138 were
observed: in the planktonic state, genes responsible by the methionine and lysine
biosynthesis are up-regulated, and genes related with the transmembrane transport
are down-regulated. And, finally, the cells exposed to AgNPs responded differently
in relation to the Ag+ ion, indi ating that the AgNPs might present a different
mechanism of action. All these results may help guide therapeutic decisions with
formulation containing AgNPs or AgNPs-CaGP associated or not with different
compounds in patients with dental caries and oral candidiasis(AU)
AN - rayyan-553781419
AU - Souza, José Antonio Santos
KW - Candidíase Bucal
Cárie Dentária
Dental Caries
Nanotechnology
Nanotecnologia
Oral Candidiasis
Prata
Silver
Toxicidade
Toxicity
LA - pt
PY - 2018
SP - 186-186
ST - Nanopartículas de prata obtidas pela síntese 'green' combinadas ou não com
glicerofosfato de cálcio e tirosol: efeito antimicrobiano, anti-inflamatório e na
resposta transcriptômica em patógenos orais
TI - Nanopartículas de prata obtidas pela síntese 'green' combinadas ou não com
glicerofosfato de cálcio e tirosol: efeito antimicrobiano, anti-inflamatório e na
resposta transcriptômica em patógenos orais
UR - https://repositorio.unesp.br/handle/11449/165285
ID - 9360
ER -
TY - JOUR
AB - Evolution of metal implants progressively shifted the focus from adequate
mechanical strength to improved biocompatibility and absence of toxicity and,
finally, to fast osseointegration. Recently, new frontiers and challenges of Ti
implants have been addressed to improvement of bioactivity, fighting of bacterial
infection and biofilm formation, as well as modulation of inflammation. This is
closely related to the clinical demand of multifunctional implants able to
simultaneously have a number of specific responses with respect to body fluids,
cells (osteoblasts, fibroblasts, macrophages) and pathogenic agents (bacteria,
viruses). This complex system of multiple biological stimuli and surface responses
is a major arena of the current research on biomaterials and biosurfaces. This
review covers the strategies explored to this purpose since 2010 in the case of Ti
and Ti alloys, considering that the number of related papers doubled about in the
last seven years and no review has comprehensively covered this engaging research
area yet. The different approaches followed for producing multifunctional Ti-based
surfaces involve the use of thick and thin inorganic coatings, chemical surface
treatments, and functionalization strategies coupled with organic coatings.
Statement of Significance According to the clinical demand of multifunctional
implants able to simultaneously have a number of specific responses with respect to
body fluids, cells and pathogenic agents, new frontiers of Ti implants have been
addressed to improvement of bioactivity, fighting of bacterial infection and
biofilm formation, as well as modulation of inflammation. Literature since 2010 is
here reviewed. Several strategies for getting bioactive and antibacterial actions
on Ti surfaces have been suggested, but they still need to be optimized with
respect to several concerns. A further step will be to combine on the same surface
a proven ability of modulation of inflammatory response. The achievement of
multifunctional surfaces able to modulate inflammation and to promote osteogenesis
is a grand challenge. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - rayyan-553781421
AU - Spriano, S.
AU - Yamaguchi, S.
AU - Baino, F.
AU - Ferraris, S.
DO - 10.1016/j.actbio.2018.08.013
KW - Titanium
Bacteria
PY - 2018
SN - 1742-7061 1878-7568
SP - 1-22
ST - A critical review of multifunctional titanium surfaces: New frontiers for
improving osseointegration and host response, avoiding bacteria contamination
TI - A critical review of multifunctional titanium surfaces: New frontiers for
improving osseointegration and host response, avoiding bacteria contamination
VL - 79
Y2 - 10 y3 - 1
ID - 9362
ER -
TY - JOUR
AB - Tumour Necrosis Factor (TNF-α) is a pro-inflammatory cytokine having key
roles in cell death, differentiation, survival, proliferation, migration and is a
modulator of immune system. Therefore, TNF-α is an ideal biomarker for several
disease diagnosis including cancer. However, out of all the biomarkers of cancer,
TNF-α) is less explored for cancer detection. Only a few reports are available of
developing biosensors for TNF-α targeting in human serum samples. Also, Carbon Dots
(CDs) remains less explored in biosensor application. In this regard, for the first
time, a sensitive and low-cost electrochemical biosensor based on CDs has
developed. CDs were synthesized by simple yet facile microwave pyrolysis. Poly
methyl methacrylate (PMMA) was selected as the matrix to hold CDs to fabricate the
biosensing platform. This novel CD-PMMA nanocomposite featuring excellent
biocompatibility, exceptional electrocatalytic conductivity, and large surface
area. CD-PMMA was applied as transducing material to efficiently conjugate
antibodies specific towards TNF-α and fabricate electrochemical immunosensor for
specific detection of TNF-α. The fabricated immunosensor was used for the detection
of TNF-α within a wide dynamic range of 0.05–160 pg mL−1 with a lower detection
limit of 0.05 pg mL−1 and sensitivity of 5.56 pg mL−1 cm−2. Furthermore, this CDs
based immunosensor retains high sensitivity, selectivity, and stability. This
immunosensor demonstrated a high correlation with the conventional technique,
Enzyme-Linked Immunosorbent Assay for early screening of cancer patient serum
samples. © 2021 Elsevier B.V.
AN - rayyan-553781422
AU - Sri, S.
AU - Lakshmi, G. B. V. S.
AU - Gulati, P.
AU - Chauhan, D.
AU - Thakkar, A.
AU - Solanki, P. R.
DO - 10.1016/j.aca.2021.338909
KW - Cancer diagnosis
Carbon dots
Composite matrix
Electrochemical biosensor
PMMA
TNF-α
Carbon
Electrochemical Techniques
Humans
Immunoassay
Limit of Detection
Neoplasms
Biocompatibility
Biomarkers
Body fluids
Cell death
Chemical detection
Diagnosis
Esters
Immunosensors
1 (3 dimethylaminopropyl) 3 ethylcarbodiimide
acetic acid
biological marker
carbon
citric acid
cysteine
ferric ferrocyanide
formic acid
n hydroxysuccinimide
nanocomposite
potassium ferricyanide
silver chloride
sodium chloride
Cancer patients
Composite matrices
Poly(methyl methacrylate)
Poly-methyl methacrylates
Serum samples
Simple++
adult
amperometry
Article
biocompatibility
calibration
calorimetry
cancer diagnosis
cancer survival
cell death
cell migration
cell proliferation
cell survival
centrifugation
chemical parameters
contact angle
controlled study
electrochemical analysis
female
human
immobilization
immune system
impedance spectroscopy
kinetics
limit of detection
male
malignant neoplasm
microwave radiation
mouth cancer
nonhuman
normal human
pH
photoluminescence
pyrolysis
Raman spectrometry
sensitivity and specificity
genetic procedures
immunoassay
neoplasm
Diseases
PY - 2021
ST - Simple and facile carbon dots based electrochemical biosensor for TNF-α
targeting in cancer patient's sample
T2 - Analytica Chimica Acta
TI - Simple and facile carbon dots based electrochemical biosensor for TNF-α
targeting in cancer patient's sample
85113484328&doi=10.1016%2fj.aca.2021.338909&partnerID=40&md5=93ad9664347760607a197a
bc307ddd20
VL - 1182
ID - 9363
ER -
TY - JOUR
AB - Human health is severely hampered by a majority of the neurological disorders
such as the brain tumors, degenerative Alzheimer's disease, Parkinson's disease and
those involving inflammatory component. Owing to the stringent protection offered
by the blood brain barrier, conventional therapeutics gain limited access and
therefore, are therapeutically suboptimal. Hence, research has now focused to
develop the novel drug delivery systems with a prime motto of maintaining
therapeutic drug levels inside the brain, avoiding non-specific tissue
distribution. The introduction of nanotechnology has addressed few of these
objectives and opened up new avenues for even more improvization. To some extent,
nanodelivery systems were successful in crossing the blood brain barrier and
accessing the remote areas of the brain. They also have shown tremendous potential
in delivering the therapeutic and diagnostic aids following systemic
administration. What revolutionised the nano applications is the development of
"smart" nanosystems, whose surface is tailor made for the effective theranostic
delivery. However, a detailed understanding of the long term nanoformulation
toxicities, along with the neuropathology, is the critical future question to be
addressed. In this review, a brief introduction of the prominent neurological
disorders and detailed applications of nanotechnology are discussed. © 2014 Bentham
Science Publishers.
AN - rayyan-553781423
AU - Sriramoju, B.
AU - Kanwar, R. K.
AU - Kanwar, J. R.
DO - 10.2174/0929867321666140716095644
IS - 36
KW - Blood brain barrier
Nanomedicine
Neurological disorders
Polymeric nanoparticles
Targeted therapeutics
Blood-Brain Barrier
Drug Carriers
Humans
Nanoparticles
Nervous System Diseases
Polymers
abs 75
benzyloxycarbonylaspartylglutamylvalylaspartyl fluoromethyl ketone
carbon nanofiber
carbon nanotube
dendrimer
dextran magnetite
doxorubicin
glial cell line derived neurotrophic factor
glutamate receptor antagonist
gold nanoparticle
liposome
metal nanoparticle
molecular scaffold
monoclonal antibody
monocrystalline iron oxide nanoparticle
nanocapsule
nanoliposome
nanoparticle
nanosphere
nerve growth factor
paclitaxel
penicillamine
polyamidoamine
polymer
silver nanoparticle
transactivator protein
ultrasmall superparamagnetic iron oxide
unclassified drug
unindexed drug
drug carrier
adsorption
Alzheimer disease
Article
blood brain barrier
cerebrovascular accident
covalent bond
drug brain level
drug formulation
drug mechanism
drug penetration
drug structure
drug targeting
drug transport
gel
glioma
human
micelle
multiple sclerosis
nanoformulation
nanogel
nanomedicine
nanomicelle
nanosuspension
nerve regeneration
neuroimaging
neurologic disease
neuropathology
nonhuman
oxidative stress
Parkinson disease
suspension
tumor localization
chemistry
metabolism
PY - 2014
SP - 4154-4168
ST - Nanomedicine based nanoparticles for neurological disorders
T2 - Current Medicinal Chemistry
TI - Nanomedicine based nanoparticles for neurological disorders
84928654680&doi=10.2174%2f0929867321666140716095644&partnerID=40&md5=2a606634350275
fd67415b7177830821
VL - 21
ID - 9364
ER -
TY - JOUR
AB - Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used
nanomaterials. Following oral exposure, these NPs can accumulate in various organs
and induce the toxicity due to their physiochemical characteristics. In present
study to reduce the toxicity, surface engineered ZnO NPs (c-ZnO NPs) were in-situ
synthesized by using polyacrylamide grafted guar gum (PAm-g-GG) polymer in alkaline
media. Further, the comparative effect of bared ZnO NPs (b-ZnO NPs) and c-ZnO NPs
were assessed on secondary target organ liver and kidneys of Swiss mice at doses of
10, 50 and 300 mg/kg following 28 days repeated oral treatment. The b-ZnO NPs were
incited severe damages in liver and kidney tissue than c-ZnO NPs as seen by
transmission electron microscopy and histopathology. The increased levels of serum
biomarkers (AST, ALT, ALP, creatinine, uric acid, and urea) were also observed,
that remarking a disturbance in the function of liver and kidney. After sub-acute
oral treatment of b-ZnO NPs, the hepatic pro-inflammatory cytokines (IL-6, TNF-
alpha, and MMP-9) were up-regulated that causes the activation of acute phase
response (APR). We also observed significantly increased in expression of hepatic
acute phase proteins (hepcidin and haptoglobin) and altered interlinked iron (Fe)
signaling biomarkers (hephaestin, IT, TFR-1, LDH, and ferroportin). This study
emphasizes that exposure to ZnO NPs may cause inflammation mediated APR through
ultra-structural damage of tissue that could escort the progression of anemia.
Nevertheless, the capping with PAm-g-GG in c- ZnO NPs has reduced the toxicity by
altering the surface reactive property of ZnO NPs.
AN - rayyan-553781424
AU - Srivastav, A. K.
AU - Dhiman, N.
AU - Tiwari, R.
AU - Arjaria, N.
AU - Prakash, J.
AU - Jagdale, P.
AU - Ayanur, A.
AU - Singh, D.
AU - Patnaik, S.
AU - Kumar, M.
DO - 10.1016/j.jtemb.2019.01.008
KW - Oxalic Acid
Zinc
Acute-Phase Reaction
Norisoprenoids
Iron
PY - 2019
SN - 0946-672X
SP - 270-287
ST - Sub-acute oral exposure of zinc oxide nanoparticles causes alteration in iron
homeostasis through acute phase response: A protective effect by surface
modification
T2 - JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
TI - Sub-acute oral exposure of zinc oxide nanoparticles causes alteration in iron
homeostasis through acute phase response: A protective effect by surface
modification
VL - 52
ID - 9365
ER -
TY - JOUR
AB - Nickel titanium (NiTi, or Nitinol) alloy is used in several biomedical
applications, including cardiac, peripheral vascular, and fallopian tube stents.
There are significant biocompatibility issues of metallic implants to nickel ions
and nano-/micro-sized alloy particles. Our laboratories have recently shown that
microscale CoCr wear particles from metal-on-metal hips crosslink with the innate
immune signaling Toll-like receptor 4 (TLR4), prompting downstream signaling that
results in interleukin (IL)-1 beta and IL-8 gene expression. In vivo, NiTi alloy
can also generate wear particles on the nanoscale (NP) that have thus far not been
studied for their potential to induce inflammation and angiogenesis that can, in
turn, contribute to implant (e.g. stent) failure. Earlier studies by others
demonstrated that nickel could induce contact hypersensitivity by crosslinking the
human, but not the mouse, TLR4. In the present work, it is demonstrated that NiCl2
ions and NiTi nanoparticles induce pro-inflammatory and pro-angiogenic
cytokine/chemokine expression in human endothelial and monocyte cell lines in
vitro. These observations prompt concerns about potential mechanisms for stent
failure. The data here showed a direct correlation between intracellular uptake of
Ni2+ and generation of reactive oxygen species. To determine a role for nickel and
NiTi nanoparticles in inducing angiogenesis in vivo, 1-cm silicone angioreactors
were implanted subcutaneously into athymic (T-cell-deficient) nude mice. The
angioreactors contained Matrigel (a gelatinous protein mixture that resembles
extracellular matrix) in addition to one of the following: PBS (negative control),
VEGF/FGF-2 (positive control), NiCl2, or NiTi NP. The implantation of angioreactors
represents a potential tool for quantification of angiogenic potentials of medical
device-derived particles and ions in vivo. By this approach, NiTi NP were found to
be markedly angiogenic, while Ni2+ was less-so. The angioreactors may provide a
powerful tool to examine if debris shed from medical devices may promote untoward
biological effects.
AN - rayyan-553781425
AU - Srivastava, A. K.
AU - Snapper, D. M.
AU - Zheng, J. W.
AU - Yildrim, B. S.
AU - Srivastava, S.
AU - Wood, S. C.
DO - 10.1080/1547691X.2022.2080307
IS - 1
KW - Inflammation
Nickel
PY - 2022
SN - 1547-691X 1547-6901
SP - 61-73
ST - Examining the role of nickel and NiTi nanoparticles promoting inflammation
and angiogenesis
T2 - JOURNAL OF IMMUNOTOXICOLOGY
TI - Examining the role of nickel and NiTi nanoparticles promoting inflammation
and angiogenesis
VL - 19
Y2 - 12 y3 - 31
ID - 9366
ER -
TY - JOUR
AB - With tremendous increase in development of nanotechnology, there is a
developing enthusiasm toward the application of nanoparticles in diverse areas.
Carbon nanotubes, fullerenes, quantum dots, dendrimers, iron oxide, silica, and
gold and silver nanoparticles are frequently used in different applications such as
drug delivery, ceramic materials, semiconductors, electronics, medicine, cosmetics,
etc. Some of these nanoparticles have shown major toxic effects on fauna, flora,
and human beings, such as inflammation, cytotoxicity, tissue ulceration, and
reduction of cell viability. Single-walled carbon nanotubes (SWCNTs) and
multiwalled carbon nanotubes (MWCNTs) can induce oxidative stress and fibrosis in
the lungs of rat and mice. SWCNTs can also induce oxidative stress to the nervous
system in human beings. Inflammatory injury and respiratory distress can be
observed due to TiO2 nanoparticles with small diameter. Nanoparticles can also pose
detrimental effects on plants, such as decreased growth rate, genomic and proteomic
changes, etc. Toxicity of nanoparticles arises because of their specific
characteristics, such as greater 'surface area to volume ratio' compared with bulk
particles of the same chemistry. The objective of this review is to critically
evaluate the current literature on the toxicity of nanoparticles. © 2015 American
Chemical Society.
AN - rayyan-553781426
AU - Srivastava, V.
AU - Gusain, D.
AU - Sharma, Y. C.
DO - 10.1021/acs.iecr.5b01610
IS - 24
KW - Carbon
Ceramic materials
Nanoparticles
Nanotechnology
Semiconductor quantum dots
Silver
Single-walled carbon nanotubes (SWCN)
Toxic materials
Toxicity
Yarn
Cell viability
Critical review
Multiwalled carbon nanotube (MWCNTs)
Single-walled carbon nanotube (SWCNTs)
Toxic effect
Volume ratio
Multiwalled carbon nanotubes (MWCN)
PY - 2015
SP - 6209-6233
ST - Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles
T2 - Industrial and Engineering Chemistry Research
TI - Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles
84933056662&doi=10.1021%2facs.iecr.5b01610&partnerID=40&md5=b2b4d3a12b6c2001f4f54ab
8710b5952
VL - 54
ID - 9367
ER -
TY - JOUR
AB - The cardiovascular response to xenobiotic particle exposure has been
increasingly studied over the last two decades, producing an extraordinary scope
and depth of research findings. With the flourishing of nanotechnology, the term
"xenobiotic particles" has expanded to encompass not only air pollution particulate
matter (PM) but also anthropogenic particles, such as engineered nanomaterials
(ENMs). Historically, the majority of research in these fields has focused on
pulmonary exposure and the adverse physiological effects associated with a host
inflammatory response or direct particle-tissue interactions. Because these
hypotheses can neither account entirely for the deleterious cardiovascular effects
of xenobiotic particle exposure nor their time course, the case for substantial
neurological involvement is apparent. Indeed, considerable evidence suggests that
not only is neural involvement a significant contributor but also a reality that
needs to be investigated more thoroughly when assessing xenobiotic particle
toxicities. Therefore, the scope of this review is several-fold. First, we provide
a brief overview of the major anatomical components of the central and peripheral
nervous systems, giving consideration to the potential biologic targets affected by
inhaled particles. Second, the autonomic arcs and mechanisms that may be involved
are reviewed. Third, the cardiovascular outcomes following neurological responses
are discussed. Lastly, unique problems, future risks, and hurdles associated with
xenobiotic particle exposure are discussed. A better understanding of these neural
issues may facilitate research that in conjunction with existing research, will
ultimately prevent the untoward cardiovascular outcomes associated with PM
exposures and/or identify safe ENMs for the advancement of human health.
AN - rayyan-553781428
AU - Stapleton, P. A.
AU - Abukabda, A. B.
AU - Hardy, S. L.
AU - Nurkiewicz, T. R.
DO - 10.1152/ajpheart.00546.2015
IS - 10
KW - Xenobiotics
PY - 2015
SN - 0363-6135 1522-1539
SP - H1609-H1620
ST - Xenobiotic pulmonary exposure and systemic cardiovascular response via
neurological links
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
TI - Xenobiotic pulmonary exposure and systemic cardiovascular response via
neurological links
VL - 309
Y2 - 11 y3 - 15
ID - 9369
ER -
TY - JOUR
AB - Purpose: Periodontal disease (PD), defined as oral inflammation caused by
dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment
options are limited. In this study, we designed, synthesized, and characterized
silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or
metronidazole (AgNPs-PEG-MET) to determine whether they can be used to treat PDs.
Materials and Methods: AgNPs were synthesized and characterized by transmission
electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic
light scattering. We determined the safety and the antimicrobial and anti-
inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis.
Antimicrobial properties were determined by measuring the minimum inhibitory
concentration (MIC) and minimum biofilm eradication concentration (MBEC) on
reference strains of bacteria and fungi. Human gingival fibroblast (HGF-1), murine
macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the
study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels
were measured using an enzyme-linked immunosorbent assay; metalloproteinase
expression was measured using Western blotting. Results: The synthesized AgNPs were
spherical and narrow-dispersed with an average diameter of 13.4 nm +/- 3.0 nm in
the case of AgNPs-CHL and 3.72 nm +/- 0.72 nm in the case of AgNPs-PEGMET. Both
types of AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a
more potent antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-
MET; however, both demonstrated beneficial properties in nontoxic concentrations.
AgNPs-CHL and AgNPs-PEG-MET decreased the production of proinflammatory cytokines
IL-1 beta, IL-6, IL-8 and TNF alpha. Both agents also decreased the levels of
metalloproteinases MMP3 and MMP8, which may indicate that they will inhibit tissue
degradation. Conclusion: AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic
options for PD, as they have antibacterial and anti-inflammatory properties.
However, to fully understand the potential of AgNPs, our in vitro findings must be
evaluated in an in vivo model.
AN - rayyan-553781431
AU - Steckiewicz, K. P.
AU - Cieciorski, P.
AU - Barcinska, E.
AU - Jaskiewicz, M.
AU - Narajczyk, M.
AU - Bauer, M.
AU - Kamysz, W.
AU - Megiel, E.
DO - 10.2147/IJN.S339046
KW - Metronidazole
Periodontics
Nanoparticles
PY - 2022
SN - 1178-2013
SP - 495-517
ST - Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery
Platforms: Their Potential Use in Treating Periodontitis
TI - Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery
VL - 17
ID - 9372
ER -
TY - JOUR
AB - PURPOSE: Periodontal disease (PD), defined as oral inflammation caused by
dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment
options are limited. In this study, we designed, synthesized, and characterized
silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or
metronidazole (AgNPs-PEG-MET) to determine whether they can be used to treat PDs.
MATERIALS AND METHODS: AgNPs were synthesized and characterized by transmission
electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic
light scattering. We determined the safety and the antimicrobial and anti-
inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis.
Antimicrobial properties were determined by measuring the minimum inhibitory
concentration (MIC) and minimum biofilm eradication concentration (MBEC) on
reference strains of bacteria and fungi. Human gingival fibroblast (HGF-1), murine
macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the
study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels
were measured using an enzyme-linked immunosorbent assay; metalloproteinase
expression was measured using Western blotting. RESULTS: The synthesized AgNPs were
spherical and narrow-dispersed with an average diameter of 13.4 nm ± 3.0 nm in the
case of AgNPs-CHL and 3.72 nm ± 0.72 nm in the case of AgNPs-PEG-MET. Both types of
AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a more potent
antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-MET; however,
both demonstrated beneficial properties in nontoxic concentrations. AgNPs-CHL and
AgNPs-PEG-MET decreased the production of proinflammatory cytokines IL-1β, IL-6,
IL-8 and TNFα. Both agents also decreased the levels of metalloproteinases MMP3 and
MMP8, which may indicate that they will inhibit tissue degradation. CONCLUSION:
AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic options for PD, as they
have antibacterial and anti-inflammatory properties. However, to fully understand
the potential of AgNPs, our in vitro findings must be evaluated in an in vivo
model.
AN - rayyan-553781820
AU - Steckiewicz, K. P.
AU - Cieciórski, P.
AU - Barcińska, E.
AU - Jaśkiewicz, M.
AU - Narajczyk, M.
AU - Bauer, M.
AU - Kamysz, W.
AU - Megiel, E.
DO - 10.2147/IJN.S339046
KW - Animals
Chlorhexidine
Humans
Metronidazole
Mice
*Periodontitis/drug therapy
*Pharmaceutical Preparations
Silver
Periodontics
Nanoparticles
LA - eng
N1 - Department of Pharmaceutical Pathophysiology, Faculty of Pharmacy, Medical
University of Gdansk, Gdansk, Poland.; Faculty of Chemistry, University of Warsaw,
Warsaw, Poland.; Department of Pharmaceutical Pathophysiology, Faculty of Pharmacy,
Medical University of Gdansk, Gdansk, Poland.; Department of Inorganic Chemistry,
Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland.; Laboratory of
Electron Microscopy, Faculty of Biology, University of Gdansk, Gdansk, Poland.;
Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of
Gdansk, Gdansk, Poland.; Department of Inorganic Chemistry, Faculty of Pharmacy,
Medical University of Gdansk, Gdansk, Poland.; Faculty of Chemistry, University of
Warsaw, Warsaw, Poland.; Department of Pharmaceutical Pathophysiology, Faculty of
Pharmacy, Medical University of Gdansk, Gdansk, Poland.
PY - 2022
SP - 495-517
ST - Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery
TI - Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery
VL - 17
ID - 9747
ER -
TY - CHAP
AB - The potent antimicrobial properties of nanoparticulate silver (AgNPs) have
led to broad interest in using them in a wide range of commercial and medical
applications. Although numerous in vivo and in vitro studies have provided evidence
of toxic effects, rapid commercialization of AgNP-based nanomaterials has advanced
without characterization of their potential environmental and health hazards. There
is evidence that AgNPs can be translocated from the blood to the brain, regardless
the route of exposure, and accumulate in the brain over time. As the brain is
responsible for basic physiological functions and controls all human activities, it
is important to assess the hazardous influence of AgNPs released from widely used
nanoproducts and possible side effects of AgNP-based therapies. A number of studies
have suggested that the size, shape and surface coating, as well as rates of silver
ion release and interactions with proteins are the key factors determining the
neurotoxicity of AgNPs. AgNPs target endothelial cells forming the blood-brain
barrier, neurons and glial cells and leads finally to oxidative stress-related cell
death. In this chapter, we review in detail current data on the impact of AgNPs on
the central nervous system and discuss the possible mechanisms of their neurotoxic
effects.
AN - rayyan-553781434
AU - Struzynska, L.
AU - Skalska, J.
AU - Saquib, Q.
AU - Faisal, M.
AU - AlKhedhairy, A. A.
AU - Alatar, A. A.
DO - 10.1007/978-3-319-72041-8_14 10.1007/978-3-319-72041-8
PY - 2018
SN - 0065-2598 2214-8019 978-3-319-72041-8 978-3-319-72040-1
SP - 227-250
ST - Mechanisms Underlying Neurotoxicity of Silver Nanoparticles
T2 - CELLULAR AND MOLECULAR TOXICOLOGY OF NANOPARTICLES
TI - Mechanisms Underlying Neurotoxicity of Silver Nanoparticles
VL - 1048
ID - 9375
ER -
TY - JOUR
AB - Edible materials have attracted increasing attention because of their
excellent properties including availability, biocompatibility, biological activity,
and biodegradability. Natural polysaccharides, phenolic compounds, and proteins are
widely used in tissue regeneration. To better characterize their healing effect,
this review article describes the applications of edible materials in tissue
regeneration including wound healing and bone tissue regeneration. As an
introduction to the topic, their sources and main bioactive properties are
discussed. Then, the mechanism by which they facilitate wound healing based on
their hemostasis, antibacterial, anti-inflammatory, and antioxidant properties is
systematically investigated. Moreover, a more comprehensive discussion is presented
on the approaches by which edible materials can be used as scaffolds or agents for
the provision of the components of natural bones for regulating the level of
osteogenesis-related cytokines to enhance bone repair. Finally, the prospects of
edible materials for tissue regeneration are discussed. © 2021 Wiley-VCH GmbH
AN - rayyan-553781435
AU - Su, X.
AU - Xian, C.
AU - Gao, M.
AU - Liu, G.
AU - Wu, J.
DO - 10.1002/mabi.202100114
IS - 8
KW - bone regeneration
edible materials
tissue engineering
wound healing
Bone and Bones
Bone Regeneration
Tissue Engineering
Tissue Scaffolds
Wound Healing
Bioactivity
Biocompatibility
Biodegradability
Tissue regeneration
alginic acid
chitin
chitosan
curcumin
cytokine
oxidized cellulose
phenol derivative
polysaccharide
protein
silver nanoparticle
biomaterial
Anti-inflammatories
Antioxidant properties
Bioactive properties
Bone tissue regeneration
Natural bone
Natural polysaccharide
Phenolic compounds
Wound healing
bone development
bone remodeling
bone tissue
chronic wound
diabetic wound
hemostasis
human
nonhuman
pathophysiology
Review
skin injury
tissue regeneration
bone
tissue scaffold
Tissue
Regeneration
PY - 2021
ST - Edible Materials in Tissue Regeneration
T2 - Macromolecular Bioscience
TI - Edible Materials in Tissue Regeneration
85107771820&doi=10.1002%2fmabi.202100114&partnerID=40&md5=b1896f364bec0ff54ff487802
6c08ec8
VL - 21
ID - 9376
ER -
TY - JOUR
AB - Myeloproliferative neoplasms (MPNs) are developing resistance to therapy by
JAK1/2 inhibitor ruxolitinib. To explore the mechanism of ruxolitinib's limited
effect, we examined the JAK1/2 mediated induction of proliferation related ERK1/2
and AKT signaling by proinflammatory interleukin-6 (IL-6) in MPN granulocytes and
JAK2V617F mutated human erythroleukemia (HEL) cells. We found that JAK1/2 or JAK2
inhibition prevented the IL-6 activation of STAT3 and AKT pathways in polycythemia
vera and HEL cells. Further, we showed that these inhibitors also blocked the IL-6
activation of the AKT pathway in primary myelofibrosis (PMF). Only JAK1/2 inhibitor
ruxolitinib largely activated ERK1/2 signaling in essential thrombocythemia and PMF
(up to 4.6 fold), with a more prominent activation in JAK2V617F positive
granulocytes. Regarding a cell cycle, we found that IL-6 reduction of HEL cells
percentage in G2M phase was reversed by ruxolitinib (2.6 fold). Moreover,
ruxolitinib potentiated apoptosis of PMF granulocytes (1.6 fold). Regarding DNA
replication, we found that ruxolitinib prevented the IL-6 augmentation of MPN
granulocytes frequency in the S phase of the cell cycle (up to 2.9 fold). The
inflammatory stimulation induces a cross-talk between the proliferation linked
pathways, where JAK1/2 inhibition is compensated by the activation of the ERK1/2
pathway during IL-6 stimulation of DNA replication.
AN - rayyan-553781436
AU - Suboticki, T.
AU - Ajtic, O. M.
AU - Beleslin-Cokic, B. B.
AU - Bjelica, S.
AU - Djikic, D.
AU - Diklic, M.
AU - Lekovic, D.
AU - Gotic, M.
AU - Santibanez, J. F.
AU - Noguchi, C. T.
AU - Cokic, V. P.
DO - 10.1002/cbin.11084
IS - 2
DNA Replication
DNA, B-Form
DNA, A-Form
PY - 2019
SN - 1065-6995 1095-8355
SP - 192-206
ST - IL-6 stimulation of DNA replication is JAK1/2 mediated in cross-talk with
hyperactivated ERK1/2 signaling
T2 - CELL BIOLOGY INTERNATIONAL
TI - IL-6 stimulation of DNA replication is JAK1/2 mediated in cross-talk with
hyperactivated ERK1/2 signaling
VL - 43
Y2 - 2
ID - 9377
ER -
TY - JOUR
AB - Platinum nanoparticles (Pt NPs) was synthesized via a facile and cost
competitive ont-pot green mediated synthesis using cell free cultural filtrate
(microgravity simulated grown Penicillium chrysogenum) as a reducing agent. The
toxicity effect of synthesized Pt NPs toward myoblast C2C12 carcinoma cells was
then investigated. The particle size analyzer (DLS) and transmission electron
microscopy (TEM) results demonstrates that both NG-Pt NPs and MG-Pt NPS are
spherical in shape with an average diameter of 15 nm and 8.5 nm, respectively. The
results from UV-visible (UV-vis) spectroscopy and X-ray diffraction (XRD) analysis
show a characteristic strong resonance centered at 265 nm and a single crystalline
nature, respectively. The results derived from in vitro cytotoxicity showed a
significant concentration-dependent decrement in cell viability when C2C12 cells
were exposed to Pt NPs. Such decrement in cell viability is because of increased
reactive oxygen species (ROS) generation. Cell apoptosis was proved by acridine
orange-ethidium bromide (AO/EtBr) dual staining, annexin V-FITC/PI-staining and
immunocytochemistry. Moreover, the protein expression of both (i) apoptosis related
proteins such as cas-3 and cas-9, (ii) inflammatory response proteins such as TNF-
alpha, TGF-beta and NF-kappa B were significantly upregulated in MG-Pt NPs treated
cells than NG-Pt NPs treated cells. Uptake and intracellular localization of MG-Pt
NPs caused by accumulation of autophagosomes in C2C12 cells and bacterial cells,
indicate that synthesized MG-Pt NPs enable for the swift cell apoptosis than NG-Pt
NPs. Interestingly, At the concentration of 40 and 80 mu g/ml MG-Pt NPs showed more
potent cytotoxicity toward cancer cells while, under identical concentration, NG-Pt
NPs exhibited rather lower cytotoxicity. Overall, our results demonstrated that MG-
Pt NPs could be selectively inhibit the growth of cancer cells via ROS-mediated
nucleus NF-kappa B and caspases activation when compared to NG-Pt NPs.
AN - rayyan-553781437
AU - Subramaniyan, S. A.
AU - Sheet, S.
AU - Vinothkannan, M.
AU - Yoo, D. J.
AU - Lee, Y. S.
AU - Belal, S. A.
AU - Shim, K. S.
DO - 10.1166/jnn.2018.14661
IS - 5
PY - 2018
SN - 1533-4880 1533-4899
SP - 3110-3125
ST - One-Pot Facile Synthesis of Pt Nanoparticles Using Cultural Filtrate of
Microgravity Simulated Grown P. chrysogenum and Their Activity on Bacteria and
Cancer Cells
T2 - JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
TI - One-Pot Facile Synthesis of Pt Nanoparticles Using Cultural Filtrate of
Microgravity Simulated Grown P. chrysogenum and Their Activity on Bacteria and
Cancer Cells
VL - 18
Y2 - 5
ID - 9378
ER -
TY - JOUR
AB - Silver nanoparticles (GO@AgNPs) were biogenically produced using Methanolic
Extract of Bark (MEB) of the plant Grewia orbiculata. Initially GO@AgNPs were
synthesised and characterised using UV-spectrometer. The physicochemical stability
was determined to be robust and unaltered in terms of pH, temperature, and salinity
changes. GO@AgNPs were also evaluated for their biological potential. The
nanoparticles displayed strong radical scavenging capacities in the 2,2-
diphenylpicrylhydrazyl (DPPH) and 2,2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic
Acid (ABTS) assays, with IC50 values of 4.5 µg/mL and 3.5 µg/mL, respectively. The
Ferric Reducing Antioxidant Power Assay (FRAP) assay revealed that it has
outstanding metal chelating activity, with a value of 6 µg/mL. The GO@AgNPs were
found to have excellent and enhanced antimicrobial potency than the crude extract
against 10 human pathogens tested, namely Staphylococcus aureus, Methicillin
resistant Staphylococcus aureus, Streptococcus epidermidis, Enterococcus faecalis,
Enterococcus faecium, Escherichia coli, Acinetobacter baumannii, Pseudomonas
aeruginosa, and Mycobacterium smegmatis. It was also reported to demonstrate
remarkable anti-cancerous property against A549 lung carcinoma cells at a low
dosage of 46 µg/mL, while being non-toxic to normal cell lines such as tracheal
epithelial cells even at highest conc. of 320 µg/mL tested to assess the
cytotoxicity. They were proven to be biocompatible with plasma, as GO@AgNPs
exhibited no substantial agglutination of RBCs up to a concentration of 50 mg/mL,
and in vitro anti-inflammatory effect of GO@AgNPs revealed protection against BSA
denaturation up to a concentration of 100 mg/mL. These results indicate that
GO@AgNPs might be employed in biological and pharmacological applications. © 2023,
Indian journals. All rights reserved.
AN - rayyan-553781438
AU - Suguna, M.
AU - Umesha, S.
DO - 10.5958/0975-6892.2023.00020.5
IS - 1
KW - anti-cancerous activity
Antioxidant property
cytotoxicity
in vitro anti-inflammatory activity
2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid)
antioxidant
chlorhexidine
diclofenac
flavonoid
grewia orbiculata extract
plant extract
polyphenol
quercetin
silver nanoparticle
tannin
unclassified drug
Acinetobacter
Acinetobacter baumannii
antifungal activity
antigen antibody reaction
Article
assay
bacterium isolation
biocompatibility
biofilm
Candida albicans
cell growth
cell proliferation
cell viability
chelation
controlled study
cytotoxicity assay
denaturation
drug synthesis
Enterococcus faecium
epithelium cell
Escherichia coli
Ganoderma lucidum
human
human cell
IC50
in vitro study
low drug dose
lung carcinoma
MTT assay
Mycobacterium smegmatis
nanomedicine
nonhuman
pH
phytochemistry
salinity
PY - 2023
SP - 202-214
ST - Biogenic synthesis of silver nanoparticles with Grewia orbiculata (GO@AgNPs)
and evaluation of their bioactive potential
T2 - Medicinal Plants
TI - Biogenic synthesis of silver nanoparticles with Grewia orbiculata (GO@AgNPs)
and evaluation of their bioactive potential
85153537127&doi=10.5958%2f0975-
6892.2023.00020.5&partnerID=40&md5=dc222c02ad84a4b76015ac081a99ce11
VL - 15
ID - 9379
ER -
TY - JOUR
AB - In the current research, we decided to formulate gold nanoparticles in
aqueous medium using plant leaf aqueous extract as a new therapeutic drug for
determining the local anesthetic, anti-inflammatory and analgesic activities in the
in vivo condition. The common chemical tests i.e., UV-Visible Spectroscopy (UV-
Vis), Transmission Electron Microscopy (TEM), and Fourier Transformed Infrared
Spectroscopy (FT-IR) were used for characterizing of nanoparticles. To survey the
cytotoxicity properties of nanoparticles, MTT assay was used on the human normal
cell lines. Analgesic activity was tested in mice using various doses orally.
Acetic acid-induced writhing episodes were significantly and dose-dependently
reduced. The local anesthetic activity were tested in frog and guinea pig models,
and it was seen that in both animals, the nanoparticle produces significant local
anesthetic activity. At the same dose, its anti-inflammatory activity was also
tested. Carrageenin-induced paw edema in mice was significantly reduced after oral
administration. It seems the recent nanoparticle may be use as a novel therapeutic
drug with local anesthetic, anti-inflammatory and analgesic activities in future.
AN - rayyan-553781439
AU - Sui, J. L.
AU - Jin, M. X.
AU - Morovvati, H.
AU - Goorani, S.
DO - 10.1016/j.inoche.2022.109642
KW - Anti-Inflammatory Agents, Non-Steroidal
Anesthetics, Local
Plant Extracts
PY - 2022
SN - 1387-7003 1879-0259
ST - Local anesthetic, anti-inflammatory and analgesic activities of nanoparticles
green-formulated by plant extract
T2 - INORGANIC CHEMISTRY COMMUNICATIONS
TI - Local anesthetic, anti-inflammatory and analgesic activities of nanoparticles
green-formulated by plant extract
VL - 143
Y2 - 9
ID - 9380
ER -
TY - JOUR
AB - Uvarianarum has anticancer, anti-inflammatory, antifungal and anti-HIV
properties which are effective in action. The aim of the study was to biosynthesize
silver nanoparticles using Uvarianarum leaves extract and determine their
anticancer activity against MCF-7 breast cancer cell line. Silver nanoparticles has
become an important prioritized nanomaterial among the others as they are non-toxic
to the cells exhibiting as a defensive inorganic antimicrobial agent. Presence of
AgNPs confirmed with yellowish-brown colour at 8:2 ratio. The synthesized
nanoparticles were characterized through SEM analysis along with Fluorescence
microscope analysis.TheEtBr/Ao staining results suggest that AgNPs may exert its
antiproliferative effect on MCF-7 cell line by suppressing its growth and inducing
apoptosis.The cytotoxic activity studied by MTT assay in MCF-7 cell lines revealed
that the U. narumAgNPs were non-toxic exhibiting 50% growth inhibition as 22.19 mu
g(/)ml.The outcomes revealed that the U.narumAgNPs as the most active, in terms of
IC50, with a more pronounced efficacy against breast cancer cell lines.
AN - rayyan-553781440
AU - Sujithra, M.
AU - Sathya, R.
AU - NirmalaDevi, P.
AU - Prema, P.
PY - 2021
SN - 2250-0480
SP - 138-143
ST - BIOSYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES FROM UVARIANARUM
EXTRACT
T2 - INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH
TI - BIOSYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES FROM UVARIANARUM
EXTRACT
Y2 - 12 y3 - 20
ID - 9381
ER -
TY - JOUR
AB - Silver nanoparticles are increasingly used in various products, due to their
antibacterial properties. Despite its wide spread use, only little information on
possible adverse health effects exists. Therefore, the aim of this study was to
assess the toxic potential of silver nanoparticles (<100 nm) in human lung
epithelial (A549) cells and the underlying mechanism of its cellular toxicity.
Silver nanoparticles induced dose and time-dependent cytotoxicity in A549 cells
demonstrated by MTT and LDH assays. Silver nanoparticles were also found to induce
oxidative stress in dose and time-dependent manner indicated by depletion of GSH
and induction of ROS, LPO, SOD, and catalase. Further, the activities of caspases
and the level of proinflammatory cytokines, namely interleukin-1β (IL-1β) and
interleukin-6 (IL-6) were significantly higher in treated cells. DNA damage, as
measured by single cell gel electrophoresis, was also dose and time-dependent
signicants in A549 cells. This study investigating the effects of silver
nanoparticles in human lung epithelial cells has provided valuable insights into
the mechanism of potential toxicity induced by silver nanoparticles and warrants
more careful assessment of silver nanoparticles before their industrial
applications. © 2013 Wiley Periodicals, Inc.
AN - rayyan-553781441
AU - Suliman Y, A. O.
AU - Ali, D.
AU - Alarifi, S.
AU - Harrath, A. H.
AU - Mansour, L.
AU - Alwasel, S. H.
DO - 10.1002/tox.21880
IS - 2
KW - Cyto-genotoxicity
Human lung epithelial cells
Oxidative stress
Proinflammatory cytokines
Apoptosis
Biological Markers
Cell Line
Cell Survival
Epithelial Cells
Humans
Inflammation
Lipid Peroxidation
Metal Nanoparticles
Mutagenicity Tests
Mutagens
Oxidative Stress
Respiratory Mucosa
Silver
Biological organs
Cell culture
Cells
Electrophoresis
Metal nanoparticles
Respiratory system
Toxicity
acetylcysteine
alpha tocopherol
ascorbic acid
caspase 3
caspase 9
glutathione
interleukin 1beta
interleukin 6
silver nanoparticle
biological marker
metal nanoparticle
mutagenic agent
silver
Adverse health effects
Cellular toxicities
Genotoxic effects
Genotoxicities
Human lung
Single cell gel electrophoresis
A549 cell line
apoptosis
Article
cell damage
cell density
cell structure
cell viability
comet assay
controlled study
cytotoxicity
DNA damage
DNA strand breakage
genotoxicity
human
human cell
inflammation
lipid peroxidation
lung alveolus epithelium cell
mitochondrion
oxidative stress
priority journal
protein expression
zeta potential
cell line
cell survival
chemically induced
cytology
drug effects
epithelium cell
metabolism
mutagen testing
pathology
respiratory tract mucosa
Humanities
Humanism
Lung
PY - 2015
SP - 149-160
ST - Evaluation of cytotoxic, oxidative stress, proinflammatory and genotoxic
effect of silver nanoparticles in human lung epithelial cells
T2 - Environmental Toxicology
TI - Evaluation of cytotoxic, oxidative stress, proinflammatory and genotoxic
effect of silver nanoparticles in human lung epithelial cells
84921612967&doi=10.1002%2ftox.21880&partnerID=40&md5=34365b570f09a096930613e2ee5aa4
8d
VL - 30
ID - 9382
ER -
TY - JOUR
AB - Nanotechnology is the study, production and controlled manipulation of
materials with a grain size < 100 nm. At this level, the laws of classical
mechanics fall away and those of quantum mechanics take over, resulting in unique
behaviour of matter in terms of melting point, conductivity and reactivity.
Additionally, and likely more significant, as grain size decreases, the ratio of
surface area to volume drastically increases, allowing for greater interaction
between implants and the surrounding cellular environment. This favourable increase
in surface area plays an important role in mesenchymal cell differentiation and
ultimately bone-implant interactions. Basic science and translational research have
revealed important potential applications for nanotechnology in orthopaedic
surgery, particularly with regard to improving the interaction between implants and
host bone. Nanophase materials more closely match the architecture of native
trabecular bone, thereby greatly improving the osseo-integration of orthopaedic
implants. Nanophase-coated prostheses can also reduce bacterial adhesion more than
conventionally surfaced prostheses. Nanophase selenium has shown great promise when
used for tumour reconstructions, as has nanophase silver in the management of
traumatic wounds. Nanophase silver may significantly improve healing of peripheral
nerve injuries, and nanophase gold has powerful anti-inflammatory effects on tendon
inflammation. Considerable advances must be made in our understanding of the
potential health risks of production, implantation and wear patterns of nanophase
devices before they are approved for clinical use. Their potential, however, is
considerable, and is likely to benefit us all in the future. ©2014 The British
Editorial Society of Bone & Joint Surgery.
AN - rayyan-553781442
AU - Sullivan, M. P.
AU - McHale, K. J.
AU - Parvizi, J.
AU - Mehta, S.
DO - 10.1302/0301-620X.96B5.33606
IS - 5
KW - Biomedical Research
Bone Substitutes
Humans
Nanomedicine
Orthopedic Procedures
Tissue Scaffolds
Drug delivery
Hydroxyapatite
Nano-orthopaedics
Nanophase
Nanotechnology
Osseo-integration
cefazolin
chromium
cobalt
fibronectin
gold nanoparticle
hydroxyapatite
molybdenum
nanocomposite
nanocrystal
nanofilm
selenium
silver nanoparticle
titanium
vitronectin
tissue scaffold
bacterium adherence
bone cell
cell surface
chemical reaction
conductance
cytotoxicity
device safety
endoprosthesis
fracture healing
health hazard
human
implant
inflammation
melting point
mesenchymal stroma cell
mesenchyme cell
molecular interaction
nanoengineering
nanotechnology
nanotoxicology
nerve regeneration
orthopedic implant
orthopedic prosthesis
orthopedic surgery
osteoblast
oxidative stress
particle size
peripheral nerve injury
postoperative infection
priority journal
prosthesis loosening
quantum mechanics
review
surface property
tendon disease
trabecular bone
translational research
wound healing
bone prosthesis
equipment
medical research
methodology
nanomedicine
PY - 2014
SP - 569-573
ST - Current concepts in orthopaedic surgery and future directions
T2 - Bone and Joint Journal
TI - Current concepts in orthopaedic surgery and future directions
84900531049&doi=10.1302%2f0301-
620X.96B5.33606&partnerID=40&md5=268204b8a2825cd4f5ce6011baab994a
VL - 96
ID - 9383
ER -
TY - JOUR
AB - Silica nanoparticles (SiNPs), one of the most produced nanoparticles (NPs) in
the world, are used in all aspects of life. The increased application of SiNPs,
especially in medicine, has raised considerable concern regarding their
toxicological impact. Previous studies have shown that SiNPs can pass through the
reproductive barrier and cause reproductive organ dysfunction by destroying Sertoli
cells, Leydig cells, and germ cells. However, little is known about the mechanism
of SiNPs-induced reproductive toxicity. In the present study, 5-week-old male mice
were intraperitoneally administered SiNPs per day for 1 week at a dose of 0.2 mg
per mouse. The results showed that SiNPs could cause damage to the structure of the
testis and the epididymis and change the reproductive organ coefficients, leading
to decreases of 56.1% and 55.3% in the rates of sperm concentration and motility
and an increase of 168.8% in the rate of sperm abnormality. Moreover, the serum
testosterone level obviously decreased from 18.77 to 5.23 mu g/ml after exposure,
and the transcription statuses of some key genes involved in the synthesis and
transport of testosterone in the testis were also affected. Additional experiments
showed that SiNPs exposure during puberty induced oxidative stress and an
inflammatory response, as shown by the changed activity of superoxide dismutase
(SOD), increased contents of malondialdehyde (MDA), and excess expression of
proinflammatory factors, including TNF-alpha and IL-1 beta. Furthermore, the
administration of SiNPs caused DNA damage and cell apoptosis, which were presented
by the increased apoptotic cells in the sections of testis and epididymis and
activation of the TNF-alpha/TNFR I-mediated pro-apoptotic pathway. In conclusion,
these results indicate that SiNPs exposure during puberty significantly damaged the
structure and function of the testis and epididymis by inducing oxidative stress
and cell apoptosis. This study provides novel insight into SiNPs-induced
reproductive toxicity during puberty, which warrants a more careful assessment of
SiNPs before their application in juvenile supplies.
AN - rayyan-553781443
AU - Sun, F. L.
AU - Wang, X. Y.
AU - Zhang, P. Z.
AU - Chen, Z. Y.
AU - Guo, Z. Y.
AU - Shang, X.
DO - 10.1007/s11356-021-18215-6
IS - 24
KW - Mice
PY - 2022
SN - 0944-1344 1614-7499
SP - 36640-36654
ST - Reproductive toxicity investigation of silica nanoparticles in male pubertal
mice
T2 - ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
TI - Reproductive toxicity investigation of silica nanoparticles in male pubertal
mice
VL - 29
Y2 - 5
ID - 9384
ER -
TY - JOUR
AB - Dengue virus (DENV) is a mosquito-transmitted single stranded RNA virus
belonging to genus Flavivirus. The virus is endemic in the tropical and subtropical
countries of the world, causing diseases classified according to symptoms and
severity (from mild to severe) as dengue fever, dengue hemorrhagic fever, and
dengue shock syndrome. Among a variety of human cell types targeted by DENV,
monocytes, macrophages, and dendritic cells are members of innate immunity, capable
of mounting rapid inflammatory responses. These cells are also major antigen
presenting cells, responsible for activating the adaptive immunity for long-term
memory. This paper is an overview of the current understanding of the following
mutually affected aspects: DENV structure, viral infectivity, cellular receptors,
innate immune response, and adaptive immunity.
AN - rayyan-553781446
AU - Sun, P. F.
AU - Kochel, T. J.
DO - 10.1155/2013/843469
PY - 2013
SN - 1537-744X
ST - The Battle between Infection and Host Immune Responses of Dengue Virus and
Its Implication in Dengue Disease Pathogenesis
T2 - SCIENTIFIC WORLD JOURNAL
TI - The Battle between Infection and Host Immune Responses of Dengue Virus and
Its Implication in Dengue Disease Pathogenesis
ID - 9387
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) have wide applications in the field of diagnosis
and treatment of diseases which are attributed to their compatibility and high
efficiency of drug delivery, because of their eco-friendly nature and easy
handling. In the current study, we have used an extract of Vitex negundo, a
traditional anti-inflammatory folk medicine in India and synthesized gold
nanoparticles (VnAuNPs), to know the effects of anti-bacterial, antioxidant, and
anti-inflammatory properties both in vivo and in vitro. V. negundo leaf extracts
(VnLE) per se yielded high flavonoids, saponins, tannins, alkaloids, glycosides,
phenols, terpenoids. The synthesized VnAuNPs in leaf extracts was confirmed by UV-
vis spectra at 543 nm and further by Fourier transform infrared spectroscopy
(FTIR), Scanning electron microscope (SEM) and through Dynamic light scattering
(DLS). The FTIR showed the peaks at 3345, 1638, 692, 683, 662 cm(-1) which are
responsible for the reduction and capping of gold nanoparticles. The size and shape
of the AuNPs were determined by SEM. DLS study analysed the particle size
distribution of AuNPs. The VnAuNPs showed significant antibacterial activity on
both gram positive and negative bacteria. VnAuNPs also showed strong antioxidant
(DPPH, H2O2 scavenging, Nitric oxide scavenging power and reducing power)
activities when compared to VnLE extract. The VnAuNPs exhibited strong anti-
inflammatory activities (COX-2, lipoxygenase and xanthine oxidase inhibitory
activity) in in vitro (HeLa cell model) and in vivo study of carrageenan induced
paw edema model in Swiss albino mice (paw edema and acetic acid-induced writhing
test). Results suggested that the synthesized VnAuNPs are potential candidates for
treating inflammatory diseases and may find application in clinical studies.
AN - rayyan-553781447
AU - Sunayana, N.
AU - Uzma, M.
AU - Dhanwini, R. P.
AU - Govindappa, M.
AU - Prakash, H. S.
AU - Raghavendra, B. V.
DO - 10.1007/s10876-019-01661-1
IS - 2
KW - Inflammation
Lipopolysaccharides
PY - 2020
SN - 1040-7278 1572-8862
SP - 463-477
ST - Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to
Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo
TI - Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to
Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo
VL - 31
Y2 - 3
ID - 9388
ER -
TY - JOUR
AB - The antimicrobial activity of silver nanoparticles has resulted in their
widespread use in many consumer products. However, despite the continuing increase
in the population exposed to silver nanoparticles, the effects of prolonged
exposure to silver nanoparticles have not been thoroughly determined. Accordingly,
this study attempted to investigate the inflammatory responses and pulmonary
function changes in rats during 90 days of inhalation exposure to silver
nanoparticles. The rats were exposed to silver nanoparticles (18 nm diameter) at
concentrations of 0.7 x 10(6) particles/cm(3) (low dose), 1.4 x 10(6)
particles/cm(3) (middle dose), and 2.9 x 10(6) particles/cm(3) (high dose) for 6
h/day in an inhalation chamber for 90 days. The lung function was measured every
week after the daily exposure, and the animals sacrificed after the 90-day exposure
period. Cellular differential counts and inflammatory measurements, such as
albumin, lactate dehydrogenase (LDH), and total protein, were also monitored in the
acellular bronchoalveolar lavage (BAL) fluid of the rats exposed to the silver
nanoparticles for 90 days. Among the lung function test measurements, the tidal
volume and minute volume showed a statistically significant decrease during the 90
days of silver nanoparticle exposure. Although no statistically significant
differences were found in the cellular differential counts, the inflammation
measurements increased in the high-dose female rats. Meanwhile, histopathological
examinations indicated dose-dependent increases in lesions related to silver
nanoparticle exposure, such as infiltrate mixed cell and chronic alveolar
inflammation, including thickened alveolar walls and small granulomatous lesions.
Therefore, when taken together, the decreases in the tidal volume and minute volume
and other inflammatory responses after prolonged exposure to silver nanoparticles
would seem to indicate that nanosized particle inhalation exposure can induce lung
function changes, along with inflammation, at much lower mass dose concentrations
when compared to submicrometer particles.
AN - rayyan-553781450
AU - Sung, J. H.
AU - Ji, J. H.
AU - Yoon, J. U.
AU - Kim, D. S.
AU - Song, M. Y.
AU - Jeong, J.
AU - Han, B. S.
AU - Han, J. H.
AU - Chung, Y. H.
AU - Kim, J.
AU - Kim, T. S.
AU - Chang, H. K.
AU - Lee, E. J.
AU - Lee, J. H.
AU - Yu, I. J.
DO - 10.1080/08958370701874671
IS - 6
KW - Lung
Rats
PY - 2008
SN - 0895-8378 1091-7691
SP - 567-574
ST - Lung function changes in Sprague-Dawley rats after prolonged inhalation
exposure to silver nanoparticles
T2 - INHALATION TOXICOLOGY
TI - Lung function changes in Sprague-Dawley rats after prolonged inhalation
exposure to silver nanoparticles
VL - 20
ID - 9391
ER -
TY - JOUR
AB - Cell signaling for T-cell growth, differentiation, and apoptosis is initiated
in the cholesterol-rich microdomains of the plasma membrane known as lipid rafts.
Herein, we investigated whether enrichment of membrane cholesterol in lipid rafts
affects antigen-specific CD4 T-helper cell functions. Enrichment of membrane
cholesterol by 40-50% following squalene administration in mice was paralleled by
an increased number of resting CD4 T helper cells in periphery. We also observed
sensitization of the Th1 differentiation machinery through co-localization of IL-2R
alpha, IL-4R alpha, and IL-12R beta 2 subunits with GM1 positive lipid rafts, and
increased STAT-4 and STAT-5 phosphorylation following membrane cholesterol
enrichment. Antigen stimulation or CD3/CD28 polyclonal stimulation of membrane
cholesterol-enriched, resting CD4 T-cells followed a path of Th1 differentiation,
which was more vigorous in the presence of increased IL-12 secretion by APCs
enriched in membrane cholesterol. Enrichment of membrane cholesterol in antigen-
specific, autoimmune Th1 cells fostered their organ-specific reactivity, as
confirmed in an autoimmune mouse model for diabetes. However, membrane cholesterol
enrichment in CD4(+) Foxp3(+) T-reg cells did not alter their suppressogenic
function. These findings revealed a differential regulatory effect of membrane
cholesterol on the function of CD4 T-cell subsets. This first suggests that
membrane cholesterol could be a new therapeutic target to modulate the immune
functions, and second that increased membrane cholesterol in various
physiopathological conditions may bias the immune system toward an inflammatory Th1
type response.
AN - rayyan-553781451
AU - Surls, J.
AU - Nazarov-Stoica, C.
AU - Kehl, M.
AU - Olsen, C.
AU - Casares, S.
AU - Brumeanu, T. D.
DO - 10.1371/journal.pone.0038733
IS - 6
KW - Lymphocytes
PY - 2012
SN - 1932-6203
ST - Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an
Inflammatory Response
T2 - PLOS ONE
TI - Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an
Inflammatory Response
VL - 7
Y2 - 6 y3 - 19
ID - 9392
ER -
TY - JOUR
AB - Oral exposure to nanomaterials is a current concern, asking for innovative
biological test systems to assess their safety, especially also in conditions of
inflammatory disorders. Aim of this study was to develop a 3D intestinal model,
consisting of Caco-2 cells and two human immune cell lines, suitable to assess
nanomaterial toxicity, in either healthy or diseased conditions. Human macrophages
(THP-1) and human dendritic cells (MUTZ-3) were embedded in a collagen scaffold and
seeded on the apical side of transwell inserts. Caco-2 cells were seeded on top of
this layer, forming a 3D model of the intestinal mucosa. Toxicity of engineered
nanoparticles (NM101 TiO2, NM300 Ag, Au) was evaluated in non-inflamed and inflamed
co-cultures, and also compared to non-inflamed Caco-2 monocultures. Inflammation
was elicited by IL-1, and interactions with engineered NPs were addressed by
different endpoints. The 3D co-culture showed well preserved ultrastructure and
significant barrier properties. Ag NPs were found to be more toxic than TiO2 or Au
NPs. But once inflamed with IL-1, the co-cultures released higher amounts of IL-8
compared to Caco-2 monocultures. However, the cytotoxicity of Ag NPs was higher in
Caco-2 monocultures than in 3D co-cultures. The naturally higher IL-8 production in
the co-cultures was enhanced even further by the Ag NPs. This study shows that it
is possible to mimic inflamed conditions in a 3D co-culture model of the intestinal
mucosa. The fact that it is based on three easily available human cell lines makes
this model valuable to study the safety of nanomaterials in the context of
inflammation.
AN - rayyan-553781452
AU - Susewind, J.
AU - Carvalho-Wodarz, C. D.
AU - Repnik, U.
AU - Collnot, E. M.
AU - Schneider-Daum, N.
AU - Griffiths, G. W.
AU - Lehr, C. M.
DO - 10.3109/17435390.2015.1008065
IS - 1
KW - Humanities
Humanism
Humans
Cell Line
PY - 2016
SN - 1743-5390 1743-5404
SP - 53-62
ST - A 3D co-culture of three human cell lines to model the inflamed intestinal
mucosa for safety testing of nanomaterials
T2 - NANOTOXICOLOGY
TI - A 3D co-culture of three human cell lines to model the inflamed intestinal
mucosa for safety testing of nanomaterials
VL - 10
Y2 - 1 y3 - 2
ID - 9393
ER -
TY - JOUR
AB - Nitinol is a nickel-titanium alloy widely used in medical devices for its
unique pseudoelastic and shape-memory properties. However, nitinol can release
potentially hazardous amounts of nickel, depending on surface manufacturing
yielding different oxide thicknesses and compositions. Furthermore, nitinol medical
devices can be implanted throughout the body and exposed to extremes in pH and
reactive oxygen species (ROS), but few tools exist for evaluating nickel release
under such physiological conditions. Even in cardiovascular applications, where
nitinol medical devices are relatively common and the blood environment is well
understood, there is a lack of information on how local inflammatory conditions
after implantation might affect nickel ion release. For this study, nickel release
from nitinol wires of different finishes was measured in pH conditions and at ROS
concentrations selected to encompass and exceed literature reports of extracellular
pH and ROS. Results showed increased nickel release at levels of pH and ROS
reported to be physiological, with decreasing pH and increasing concentrations of
hydrogen peroxide and NaOCl/HOCl having the greatest effects. The results support
the importance of considering the implantation site when designing studies to
predict nickel release from nitinol and underscore the value of understanding the
chemical milieu at the device-tissue interface.
AN - rayyan-553781455
AU - Sussman, E. M.
AU - Shi, H. Y.
AU - Turner, P. A.
AU - Saylor, D. M.
AU - Weaver, J. D.
AU - Simon, D. D.
AU - Takmakov, P.
AU - Sivan, S.
AU - Shin, H. Y.
AU - Di Prima, M. A.
AU - Godar, D. E.
DO - 10.1007/s40830-022-00364-3
IS - 2
KW - Sodium Hypochlorite
Hydrogen
Nickel
Hydrogen Peroxide
Hydrogenation
Hydrogen-Ion Concentration
PY - 2022
SN - 2199-384X 2199-3858
SP - 98-106
ST - Nitinol Release of Nickel under Physiological Conditions: Effects of Surface
Oxide, pH, Hydrogen Peroxide, and Sodium Hypochlorite
T2 - SHAPE MEMORY AND SUPERELASTICITY
TI - Nitinol Release of Nickel under Physiological Conditions: Effects of Surface
Oxide, pH, Hydrogen Peroxide, and Sodium Hypochlorite
VL - 8
Y2 - 6
ID - 9395
ER -
TY - JOUR
AB - Infection constitutes a major risk for implant failure, but the reasons why
biomaterial sites are more vulnerable than normal tissue are not fully elucidated.
In this study, a soft tissue infection model was developed, allowing the analysis
of cellular and molecular responses in each of the sub-compartments of the implant-
tissue interface (on the implant surface, in the surrounding exudate and in the
tissue). Smooth and nanostructured titanium disks with or without noble metal
chemistry (silver, gold, palladium), and sham sites, were inoculated with
Staphylococcus epidermidis and analysed with respect to number of viable bacteria,
number, viability and gene expression of host cells, and using different
morphological techniques after 4 h, 24 h and 72 h. Non-infected rats were controls.
Results showed a transient inflammatory response at control sites, whereas
bacterial administration resulted in higher recruitment of inflammatory cells
(mainly polymorphonuclear), higher, continuous cell death and higher gene
expression of tumour necrosis factor-alpha, interleukin-6, interleukin-8, Toll-like
receptor 2 and elastase. At all time points, S. epidermidis was predominantly
located in the interface zone, extra- and intracellularly, and lower levels were
detected on the implants compared with surrounding exudate. This model allows
detailed analysis of early events in inflammation and infection associated to
biomaterials in vivo leading to insights into host defence mechanisms in
biomaterial-associated infections.
AN - rayyan-553782314
AU - Svensson, S.
AU - Trobos, M.
AU - Hoffman, M.
AU - Norlindh, B.
AU - Petronis, S.
AU - Lausmaa, J.
AU - Suska, F.
AU - Thomsen, P.
J2 - Biomaterials
KW - Animals
Biocompatible Materials/*adverse effects
Cell Shape/drug effects
Colony Count, Microbial
Female
Inflammation/*pathology
Materials Testing
Microscopy, Atomic Force
Nanostructures/ultrastructure
Photoelectron Spectroscopy
Pilot Projects
Staphylococcal Infections/*microbiology/*pathology
Staphylococcus epidermidis/*drug effects/growth & development/ultrastructure
Surface Properties
Inflammation
LA - eng
N1 - BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Box 412,
405 30 Gothenburg, Sweden; Department of Biomaterials, Sahlgrenska Academy at
University of Gothenburg, Box 412, 405 30 Gothenburg, Sweden.; BIOMATCELL VINN
Excellence Center of Biomaterials and Cell Therapy, Box 412, 405 30 Gothenburg,
Sweden; Department of Biomaterials, Sahlgrenska Academy at University of
Gothenburg, Box 412, 405 30 Gothenburg, Sweden.; BIOMATCELL VINN Excellence Center
of Biomaterials and Cell Therapy, Box 412, 405 30 Gothenburg, Sweden; Department of
Biomaterials, Sahlgrenska Academy at University of Gothenburg, Box 412, 405 30
Gothenburg, Sweden.; BIOMATCELL VINN Excellence Center of Biomaterials and Cell
Therapy, Box 412, 405 30 Gothenburg, Sweden; Department of Biomaterials,
Sahlgrenska Academy at University of Gothenburg, Box 412, 405 30 Gothenburg,
Sweden.; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Box
412, 405 30 Gothenburg, Sweden; SP Technical Research Institute of Sweden, Box 857,
501 15 Borås, Sweden.; BIOMATCELL VINN Excellence Center of Biomaterials and Cell
Therapy, Box 412, 405 30 Gothenburg, Sweden; SP Technical Research Institute of
Sweden, Box 857, 501 15 Borås, Sweden.; BIOMATCELL VINN Excellence Center of
Biomaterials and Cell Therapy, Box 412, 405 30 Gothenburg, Sweden; Department of
Biomaterials, Sahlgrenska Academy at University of Gothenburg, Box 412, 405 30
Gothenburg, Sweden.; BIOMATCELL VINN Excellence Center of Biomaterials and Cell
Therapy, Box 412, 405 30 Gothenburg, Sweden; Department of Biomaterials,
Sahlgrenska Academy at University of Gothenburg, Box 412, 405 30 Gothenburg,
Sweden. Electronic address: peter.thomsen@biomaterials.gu.se.
PY - 2015
SP - 106-21
ST - A novel soft tissue model for biomaterial-associated infection and
inflammation - bacteriological, morphological and molecular observations
T2 - Biomaterials
TI - A novel soft tissue model for biomaterial-associated infection and
inflammation - bacteriological, morphological and molecular observations
VL - 41
Y2 - 2
ID - 10222
ER -
TY - JOUR
AB - Nanomaterials, substances below 100 nm, are increasingly used in medical
diagnosis and treatment every day. The use of such materials has helped deliver
drugs across the blood-brain barrier, alleviate allergy symptoms, specifically
target cancer or HIV cells, and more. However, the tunable characteristics of such
materials have not been perfected. The different materials, sizes, shapes, and
structures have different responses on the body. This paper will investigate the
successful treatments made with nanoparticles and some general health effects. A
review of the literature revealed an inflammatory response and an increased
production of reactive oxidative species (ROS) to be common immune responses to
nanomaterial use. The mechanisms by which the inflammatory response and ROS
production occur will also be discussed. © 2012 Springer Science+Business Media,
LLC.
AN - rayyan-553781459
AU - Syed, S.
AU - Zubair, A.
AU - Frieri, M.
DO - 10.1007/s11882-012-0302-3
IS - 1
KW - Advances
Allergy
Immune response
Immunology
Nanomaterials
NF-κB pathway
Nuclear factor-κB
acetylcysteine
aluminum oxide
carbon nanotube
chitosan
gamma interferon
gold nanoparticle
I kappa B alpha
interleukin 12
interleukin 6
nanomaterial
quantum dot
saquinavir
silicon dioxide
silver nanoparticle
titanium dioxide
antiviral activity
cytokine release
cytokine response
cytotoxicity
genotoxicity
human
immune response
inflammation
nanoanalysis
nanopharmaceutics
nanotoxicology
nonhuman
oxidative stress
particle size
review
silicosis
Th1 Th2 balance
toxicity testing
transcription regulation
PY - 2013
SP - 50-57
ST - Immune response to nanomaterials: Implications for medicine and literature
review
T2 - Current Allergy and Asthma Reports
TI - Immune response to nanomaterials: Implications for medicine and literature
review
84872609432&doi=10.1007%2fs11882-012-0302-
3&partnerID=40&md5=c2e3c04fd5f1575990bfdc0711e0dfbc
VL - 13
ID - 9399
ER -
TY - JOUR
AB - In recent years, there has been a significant increase in the clinical use of
organometallic compounds and metal complexes for therapeutic purposes including
treatment of inflammatory bowel diseases (IBD). Their action is based on the
inhibition of the inflow of pro-inflammatory cytokines, the elimination of free
radicals or the modulation of intestinal microbiota. In addition, these compounds
are intended for use in the diagnosis and treatment of colorectal cancer (CRC)
which is often a consequence of IBD. The aim of this study is to critically discuss
recent findings on the use of organometallic compounds and metal complexes in the
treatment of IBD and CRC and suggest future trends in drug design. © 2019 by the
authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553781460
AU - Szczepaniak, A.
AU - Fichna, J.
DO - 10.3390/biom9090398
IS - 9
KW - Colorectal cancer
Free radicals
Inflammatory bowel diseases
Metal complexes
Organometallic compounds
Animals
Carbon Monoxide
Coordination Complexes
Humans
aminosalicylic acid
carbon monoxide
epidermal growth factor receptor
free radical
gelatinase B
gold complex
gold nanoparticle
interleukin 10
interleukin 12
interleukin 1beta
interleukin 6
interleukin 8
iridium
metal complex
NEDD8 protein
organometallic compound
rhodium
ruthenium
salazosulfapyridine
silver nanoparticle
STAT3 protein
triphenyltin
unindexed drug
zinc
coordination compound
Article
cancer cell line
cancer chemotherapy
cancer radiotherapy
chemical structure
colorectal cancer
cytotoxicity
DNA damage
drug synthesis
endoscopy
enzyme activity
human
IC50
intestine flora
microbial community
nonhuman
oxidative stress
animal
colorectal tumor
metabolism
Organometallic Compounds
PY - 2019
ST - Organometallic compounds and metal complexes in current and future treatments
of inflammatory bowel disease and colorectal cancer-a critical review
T2 - Biomolecules
TI - Organometallic compounds and metal complexes in current and future treatments
of inflammatory bowel disease and colorectal cancer-a critical review
85071439981&doi=10.3390%2fbiom9090398&partnerID=40&md5=51fd985c652595c3c1a8a15c6faf
a8d4
VL - 9
ID - 9400
ER -
TY - JOUR
AB - Generation of well-defined potential metallotherapeutics for cancer
treatment, one of the most population-threatening diseases, is challenging and an
active area of modern research in view of their unique properties and thus multiple
possible pathways of action in cells. Specifically, Schiff base ligands were
recognized as very promising building blocks for the construction of stable and
active complexes of numerous geometries and topologies. Incorporation of Ag(I) ions
allows for the formation of flat complexes with potential unoccupied coordination
sites, thus giving rise to specific interactions between the metallotherapeutic and
biomolecule of interest. Herein, we present the design, synthesis and
characterization of new Schiff base ligand L and its Ag(I) bimetallic complex
[Ag2L2]2+ with two planar moieties formed around the metal ions and connected
through cyclohexane rings, confirmed by X-ray measurements. The compounds were
described in context of their potential use as anticancer drugs through DNA and BSA
binding pathways by several spectroscopic methods (CD, UV-Vis, fluorescence). We
revealed that both, L and [Ag2L2]2+, interact with similar affinity with CT-DNA
(Kb~106 M−1), while they differ in the type and strength of interactions with the
model albumin–BSA. [Ag2L2]2+ binds BSA in both a dynamic and static manner with the
Ksv = 8.8 × 104 M−1 in the Trp-134 and Trp-213 sites, whereas L interacts with BSA
only dynamically (KSV = 2.4 × 104 M−1). This found further confirmation in the CD
studies which revealed a reduction in α-helix content in the albumin of 16% in
presence of [Ag2L2]2+. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AN - rayyan-553781461
AU - Szymańska, M.
AU - Pospieszna-Markiewicz, I.
AU - Mańka, M.
AU - Insińska-Rak, M.
AU - Dutkiewicz, G.
AU - Patroniak, V.
AU - Fik-Jaskółka, M. A.
DO - 10.3390/biom11101449
IS - 10
Anticancer
BSA
CT-DNA
Schiff base
Silver(I) complex
Coordination Complexes
DNA
DNA-Binding Proteins
Humans
Ligands
Neoplasms
Protein Binding
Schiff Bases
Silver
acetic acid
albumin
cisplatin
cyclohexane
doxorubicin
scoparone
silver
thiazole derivative
coordination compound
DNA binding protein
ligand
protein binding
alpha helix
apoptosis
Article
base pairing
binding affinity
cancer therapy
carbon nuclear magnetic resonance
chemical structure
circular dichroism
computer model
crystal structure
cytotoxicity
drug solubility
drug synthesis
fluorescence
herbal medicine
hydrogen bond
hydrophobicity
inhibition constant
molecular docking
molecular recognition
spectrofluorometry
spectroscopy
static electricity
stoichiometry
thermostability
X ray
X ray diffraction
chemistry
drug effect
human
neoplasm
synthesis
DNA (Cytosine-5-)-Methyltransferase
DNA, B-Form
DNA, A-Form
PY - 2021
ST - Synthesis and spectroscopic investigations of schiff base ligand and its
bimetallic ag(I) complex as dna and bsa binders
T2 - Biomolecules
TI - Synthesis and spectroscopic investigations of schiff base ligand and its
bimetallic ag(I) complex as dna and bsa binders
85116147194&doi=10.3390%2fbiom11101449&partnerID=40&md5=965b1bbd9f399bee714738414ad
adc5f
VL - 11
ID - 9401
ER -
TY - JOUR
AB - Due to the widespread use of indium tin oxide (ITO), it is important to
investigate its effect on human health. In this study, we evaluated the cellular
effects of ITO nanoparticles (NPs), indium chloride (InCl3) and tin chloride
(SnCl3) using human lung epithelial A549 cells. Transmission electron microscopy
and inductively coupled plasma mass spectrometry were employed to study cellular
ITO NP uptake. Interestingly, greater uptake of ITO NPs was observed, as compared
with soluble salts. ITO NP species released could be divided into two types:
'indium release ITO' or 'tin release ITO'. We incubated A549 cells with indium
release ITO, tin release ITO, InCl3 or SnCl2 and investigated oxidative stress,
proinflammatory response, cytotoxicity and DNA damage. We found that intracellular
reactive oxygen species were increased in cells incubated with indium release ITO,
but not tin release ITO, InCl3 or SnCl2. Messenger RNA and protein levels of the
inflammatory marker, interleukin-8, also increased following exposure to indium
release ITO. Furthermore, the alkaline comet assay revealed that intracellular
accumulation of indium ions induced DNA damage. Our results demonstrate that the
accumulation of ionic indium, but not ionic tin, from ITO NPs in the intracellular
matrix has extensive cellular effects.
AN - rayyan-553781462
AU - Tabei, Y.
AU - Sonoda, A.
AU - Nakajima, Y.
AU - Biju, V.
AU - Makita, Y.
AU - Yoshida, Y.
AU - Horie, M.
DO - 10.1093/jb/mvv098
IS - 2
Oxidative Stress
DNA Damage
DNA, B-Form
DNA, A-Form
DNA
PY - 2016
SN - 0021-924X 1756-2651
SP - 225-237
ST - Intracellular accumulation of indium ions released from nanoparticles induces
oxidative stress, proinflammatory response and DNA damage
T2 - JOURNAL OF BIOCHEMISTRY
TI - Intracellular accumulation of indium ions released from nanoparticles induces
oxidative stress, proinflammatory response and DNA damage
VL - 159
Y2 - 2
ID - 9402
ER -
TY - JOUR
AB - Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry as a
white pigment (paints, paper industry and toothpastes), photocatalysts
(environmental decontamination and photovoltaic cells), inorganic UV filter
(sunscreens and personal care products) and as a food additive (E171) and
antimicrobial food packaging material. Silver nanoparticles (Ag NPs) are used in
photonics, microelectronics, catalysis and medicine due to their catalytic
activity, magnetic and optical polarizability, electrical and thermal
conductivities and enhanced Raman scattering. They also have antibacterial,
antifungal and antiviral activities, as well as anti-inflammatory potential. The
huge increase in the use of nano-based products, mainly metallic NPs, implies the
presence of nano-materials in the environment, and hence, the unintentional human
ingestion through water or foods (gastrointestinal tract is the main pathway of NPs
intake in humans). The presence of TiO2 NPs and Ag NPs in seafood samples was
firstly established using an ultrasound assisted enzymatic hydrolysis procedure and
sp-ICP-MS analysis. Several clams, cockles, mussels, razor clams, oysters and
variegated scallops, which contain TiO2 NPs and Ag NPs, were subjected to an in
vitro digestion process simulating human gastrointestinal digestion in the stomach
and in the small and large intestine to determine the bioaccessibility of these
NPs. Caco-2 cells were selected as model of human intestinal epithelium for
transport studies because of the development of membrane transporters that are
responsible for the uptake of chemicals. Parameters as transepithelial electrical
resistance (TEER) and permeability of Lucifer Yellow were studied for establishing
cell monolayer integrity. TiO2 NPs and Ag NPs transport as well as total Ti and Ag
concentrations passing through the gastrointestinal epithelial barrier model (0-2
h) were assessed by sp-ICP-MS and ICP-MS in several molluscs.
AN - rayyan-553781463
AU - Taboada-Lopez, M. V.
AU - Leal-Martinez, B. H.
AU - Dominguez-Gonzalez, R.
AU - Bermejo-Barrera, P.
AU - Taboada-Antelo, P.
AU - Moreda-Pineiro, A.
DO - 10.1016/j.talanta.2021.122494
KW - Humanities
Humanism
Humans
Titanium
PY - 2021
SN - 0039-9140 1873-3573
ST - Caco-2 in vitro model of human gastrointestinal tract for studying the
absorption of titanium dioxide and silver nanoparticles from seafood
T2 - TALANTA
TI - Caco-2 in vitro model of human gastrointestinal tract for studying the
absorption of titanium dioxide and silver nanoparticles from seafood
VL - 233
Y2 - 10 y3 - 1
ID - 9403
ER -
TY - JOUR
AB - Background: Nitric oxide (NO) production by the action of the inducible
nitric oxide synthase (iNOS or NOS2) is increased in tissues that are stimulated by
cytokine and endotoxins. The role of NO in the pathogenesis of chronic viral
hepatitis is not fully understood but it seems that its overproduction is
responsible for the pathological changes under inflammatory conditions. Aim: In
this paper, we analyzed the correlation between immunohistochemical expression of
iNOS and liver fibrosis in chronic viral hepatitis. Materials and Methods: Liver
biopsies from patients diagnosed with chronic viral hepatitis B and C were embedded
in paraffin and further used for histological staining and immunohistochemical
reactions to detect the expression of iNOS and TGF-beta 1. The degree of liver
fibrosis was established using special staining (trichromic Masson and Gomori's
silver impregnation). Results: In samples with low degree of fibrosis, we observed
a discrete positivity for iNOS in periportal hepatocytes and the
immunohistochemical reaction for TGF-beta 1 were limited to the endothelial cells
of liver sinusoids and pro-inflammatory cells from the portal tracts. Positive
reaction for TGF-beta 1 increased with the degree of liver fibrosis, while the
expression of iNOS was enhanced in hepatocytes, as well as in bile ducts and
endothelial cells. Conclusions: Infection with hepatitis B and C viruses induces
iNOS expression in hepatocytes, suggesting that NO overproduction might have an
important role in progression of chronic viral hepatitis to cirrhosis.
AN - rayyan-553781464
AU - Tache, D. E.
AU - Stanciulescu, C. E.
AU - Banita, I. M.
AU - Purcaru, S. O.
AU - Andrei, A. M.
AU - Comanescu, V.
AU - Pisoschi, C. G.
IS - 2
KW - Nitric Oxide Synthase
Liver
Nitric Oxide
Liver Cirrhosis
Nitric Oxide Synthase Type II
PY - 2014
SN - 1220-0522
SP - 539-543
ST - Inducible nitric oxide synthase expression (iNOS) in chronic viral hepatitis
and its correlation with liver fibrosis
T2 - ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY
TI - Inducible nitric oxide synthase expression (iNOS) in chronic viral hepatitis
and its correlation with liver fibrosis
VL - 55
ID - 9404
ER -
TY - JOUR
AB - Medical device associated infections are a persistent medical problem which
has not found a comprehensive solution yet. Over the last decades, there have been
intense research efforts toward developing antibacterial coatings that could
potentially improve medical outcomes. Silver nanoparticles have attracted a great
deal of attention as a potent alternative to conventional antibiotics. Herein, we
present a biologically inspired approach to synthesize phospholipid encapsulated
silver nanoparticles and their surface immobilization to a functional plasma
polymer interlayer to generate antibacterial coatings. The antibacterial efficacy
of the coatings was evaluated against three medically relevant pathogens including
the Gram-positive Staphylococcus aureus and Staphylococcus epidermidis, and the
Gram-negative Pseudomonas aeruginosa. The innate immune response to the coatings
was assessed in vitro using primary bone marrow derived macrophages (BMDM). Any
potential cytotoxicity was studied with primary human dermal fibroblasts (HDFs).
Overall, the coatings had excellent inhibition of bacterial growth. We also
observed reduced expression of pro-inflammatory cytokines from BMDM which suggests
a reduced inflammatory response. The combined properties of coatings developed in
this study may make them a good candidate for application on medical devices such
as catheters and wound dressings. © 2015 American Chemical Society.
AN - rayyan-553781466
AU - Taheri, S.
AU - Cavallaro, A.
AU - Christo, S. N.
AU - Majewski, P.
AU - Barton, M.
AU - Hayball, J. D.
AU - Vasilev, K.
IS - 12
KW - antibacterial coating
phospholipid bilayer
phospholipid
phosphorus
polymer
silver
silver nanoparticle
Article
bacterial growth
biofilm
bone marrow derived macrophage
cell growth
cell mediated cytotoxicity
cell viability
comparative study
conjugation
controlled study
human
human cell
immobilization
in vitro study
innate immunity
lipid bilayer
nanoencapsulation
precipitation
priority journal
surface property
synthesis
ultrasound
Polymerization
Polymers
Phospholipids
PY - 2015
SP - 1278-1286
ST - Antibacterial Plasma Polymer Films Conjugated with Phospholipid Encapsulated
T2 - ACS Biomaterials Science and Engineering
TI - Antibacterial Plasma Polymer Films Conjugated with Phospholipid Encapsulated
84962635896&doi=10.1021%2facsbiomaterials.5b00338&partnerID=40&md5=b0ae4b9e68c218a3
a2a9ce7eeedc7d1d
VL - 1
ID - 9406
ER -
TY - GEN
AB - O objetivo deste estudo foi investigar o efeito de diferentes soluções
coloidais de nanopartículas de prata sobre a viabilidade celular de fibroblastos
(linhagem L929) e sobre a resposta inflamatória de tecido subcutâneo de ratos.
Nanopartículas de prata (SNP) com tamanho médio de 5 nm foram sintetizadas através
da redução do nitrato de prata pelo citrato de sódio e estabilizadas com amônia
(SNP-A) ou polivinilpirrolidona (SNP-P). Para avaliar a viabilidade celular,
células L929 foram expostas SNP e agentes estabilizantes (amônia (NH3) e
polivinilpirrolidona (PVP)) (0,1 100 μg/mL), e após 6, 24 e 48 h foi realizado o
ensaio de citotoxicidade celular pelo método do MTT. A resposta tecidual foi
realizada com tubos de polietileno contendo SNP (1.0 μg/mL; 540 μg/mL) e agentes
estabilizantes (NH3 0.13 x 10-3 mol/L e PVP 0.19 g/L) implantados no tecido
conjuntivo dorsal de ratos Wistar por 7, 15, 30, 60 e 90 dias. Os espécimes foram
corados com hematoxilina e eosina e foram realizadas avaliações qualitativa e
quantitativa. SNP inibiram a viabilidade celular no teste in vitro de maneira
concentração-dependente. SNP-A foram mais tóxicas para L929 que as partículas
estabilizadas com PVP. O exame histológico mostrou que SNP 540 μg/mL induziram
reação tecidual significantemente mais intensa em 30 e 60 dias comparado aos grupos
controles (solução fisiológica 0,9% e fibrina) nos mesmos períodos. As respostas
inflamatórias causadas por SNP 1,0 μg/mL, NH3 0,13 x 10- 3 mol/L e PVP 0,19 g/L
foram similares aos controles em todos os períodos experimentais. Foi possível
concluir que a exposição à SNP reduziu a viabilidade de células L929 de maneira
concentração-dependente. O tipo de agente estabilizante interferiu na
citotoxicidade sendo SNP-A mais tóxica para L929. Ambos os tipos de soluções
coloidais de nanopartículas de prata (SNP-A e SNP-P) a 540 µg/mL induziram
significante resposta inflamatória no tecido subcutâneo de rato The aim of this
study was to investigate the effect of different colloidal silver nanoparticles on
cell viability of mouse fibroblasts (cell line L929) and on the subcutaneous
connective tissue reaction of rats. Silver nanoparticles (SNP) of average size 5 nm
were synthesized by the reduction of silver nitrate through sodium citrate and were
stabilized with ammonia (SNP-A) or polyvinylpyrrolidone (SNP-P). To evaluate the
cell viability, L929 cell were exposure to silver nanoparticles (0.1-100 μg/mL),
and after 6, 24 and 48h MTT assay was performed. The tissue reaction was carried
out with polyethylene tubes containing silver nanoparticles (1.0 μg/mL; 540 μg/mL)
implanted in the dorsal connective tissue of Wistar rats for 7, 15, 30, 60, and 90
days. The specimens were stained with hematoxylin and eosin and qualitative and
quantitative evaluations of the reaction were carried out. Silver nanoparticles
inhibited the cell viability in the in vitro test in a concentration-dependent
manner. SNP-A were more toxic to L929 than particles stabilized with
polyvinylpyrrolidone (PVP). Histological examination showed that SNP at 540 μg/mL
induced significant tissue reaction on 30 and 60 days after implantation compared
to the controls groups (fibrin and saline 0.9%) at the same periods. The
inflammatory responses caused by SNP at 1.0 μg/ml, NH3 at 0.13 x 10-3 mol/L and PVP
at 0.19 g/L solutions were similar to the controls groups in all experimental
periods. It was possible to conclude that SNP exposure decreased the viability of
L929 cells in a concentration-dependent manner. The type of stabilizing agent
interfered on the cytotoxicity of SNP being SNP-A more toxic to L929. Also, both
colloidal silver nanoparticles (SNP-A and SNP-P) at 540 μg/mL induced significant
inflammatory response in rats subcutaneous tissue
AN - rayyan-553781472
AU - Takamiya, Aline Satie
KW - Biocompatibilidade
Biocompatibility
Nanoparticle
Nanopartícula
Prata
Silver
Toxicidade
Toxicity
LA - en
PY - 2013
SP - 108-108
ST - Avaliação da resposta tecidual e da citotoxicidade de soluções coloidais de
nanopartículas de prata
TI - Avaliação da resposta tecidual e da citotoxicidade de soluções coloidais de
nanopartículas de prata
UR - http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/04-06-
2014/000737014.pdf
ID - 9412
ER -
TY - JOUR
AB - A new Ag(I) boron imidazolate framework with the chemical formula of
{Ag[BH(im)3]}n (1, BH(im)3− = tri(imidazolyl)borate) has been synthesized by the
self-assembly of Ag(I) and tridentate boron imidazolate ligand. This compound shows
intense luminescence and outstanding photocatalytic activity for the Rhodamine B
degradation with an irradiation of ultraviolet. Moreover, the compound’s
therapeutic effects on neonatal pneumonia were explored and the corresponding
mechanism was tested in meantime. The enzyme linked immunosorbent assay detection
was firstly conducted to measure the content of inflammatory cytokines released
into the bronchoalveolar lavage fluid. Then, the activation of JNK/MAPK signaling
pathway activation in the alveolar epithelial cells was measured with the real-time
reverse transcription-polymerase chain reaction. Finally, the cytotoxicity of the
complex was determined with Cell Counting Kit (CCK-8) assay. © 2022, The Author(s),
under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
AN - rayyan-553781473
AU - Tan, J. F.
AU - Zhao, H.
DO - 10.1007/s00289-022-04419-4
IS - 7
KW - Ag(I) compound
Cytotoxicity
Inflammatory cytokine
Neonatal pneumonia
Photocatalysis
Boron
Cell signaling
Functional polymers
Irradiation
Photocatalytic activity
Silver compounds
Chemical formulae
Coordination Polymers
Coordination-polymers
Imidazolate
Imidazolyl
Photocatalytic property
Therapeutic effects
Chemical activation
Infant, Newborn
Polymerization
Polymers
Aged
Aging
PY - 2023
SP - 7779-7790
ST - Layered Ag(I) coordination polymer: photocatalytic property and therapeutic
effects on neonatal pneumonia
T2 - Polymer Bulletin
TI - Layered Ag(I) coordination polymer: photocatalytic property and therapeutic
effects on neonatal pneumonia
85136056036&doi=10.1007%2fs00289-022-04419-
4&partnerID=40&md5=188dd6717fc54511c43d43ae86e9a687
VL - 80
ID - 9413
ER -
TY - JOUR
AB - The effective treatment for periodontitis is to completely and sustainedly
eradicate the bacterial pathogens from the complex periodontal pockets. Local
sustained-release antibiotics as a complementary treatment after scaling and root
planning can sustainedly combat bacterial pathogens in the periodontal pockets to
help treat the disease, but the increasing concern of bacterial resistance limits
its future use. Here, we reported a local antibacterial system based on microsized
multifunctional Ag-TiO2-x encapsulated in alginate (ATA) microspheres. We confirmed
that ATA displayed strong photothermally enhanced dual enzyme-mimicking
(peroxidase-like and catalase-like) activities and weak photocatalytic activity
under 808 nm near-infrared (NIR) irradiation, which could boost the generation of
reactive oxygen species (ROS) and O-2 in the presence of low-level H2O2. As a
result, the ATA/H2O2/NIR system exhibited efficient antibacterial activity against
Porphyromonas gingivalis and Streptococcus gordonii in both planktonic and biofilm
forms. With the help of ROS, ATA could release Ag+ in concentrations sufficient to
inhibit periodontal pathogens as well. Moreover, the in situ-generated oxygen was
supposed to alleviate the local hypoxic environment and would help downregulate the
lipopolysaccharide-mediated inflammatory response of periodontal stem cells. The in
vivo rat periodontitis treatment results demonstrated that the ATA/H2O2/NIR system
reduced the bacterial load, relieved inflammation, and improved tissue healing. Our
work developed a new local prolonged bactericidal and oxygenation system for
enhanced periodontitis. Avoiding the usage of antibiotics and nanomaterials, this
strategy showed great promise in adjunctive periodontitis treatment and also in
other biomedical applications.
AN - rayyan-553781474
AU - Tan, X. Z.
AU - Liu, S. R.
AU - Hu, X. Y.
AU - Zhang, R. T.
AU - Su, X. F.
AU - Qian, R. J.
AU - Mai, Y.
AU - Xu, Z. Y.
AU - Jing, W.
AU - Tian, W. D.
AU - Xie, L.
DO - 10.1021/acsami.2c17065
KW - Microspheres
Periodontics
PY - 2022
SN - 1944-8244 1944-8252
ST - Near-Infrared-Enhanced Dual Enzyme-Mimicking Ag-TiO2-x@Alginate Microspheres
with Antibactericidal and Oxygeneration Abilities to Treat Periodontitis
TI - Near-Infrared-Enhanced Dual Enzyme-Mimicking Ag-TiO2-x@Alginate Microspheres
with Antibactericidal and Oxygeneration Abilities to Treat Periodontitis
Y2 - 12 y3 - 23
ID - 9414
ER -
TY - JOUR
AB - Engineered nanomaterials (ENMs) have been widely exploited in several
industrial domains as well as our daily life, raising concern over their potential
adverse effects. While in general ENMs do not seem to have detrimental effects on
immunity or induce severe inflammation, their indirect effects on immunity are less
known. In particular, since the gut microbiota has been tightly associated with
human health and immunity, it is possible that ingested ENMs could affect
intestinal immunity indirectly by modulating the microbial community composition
and functions. In this perspective, we provide a few pieces of evidence and discuss
a possible link connecting ENM exposure, gut microbiota and host immune response.
Some experimental works suggest that excessive exposure to ENMs could reshape the
gut microbiota, thereby modulating the epithelium integrity and the inflammatory
state in the intestine. Within such microenvironment, numerous microbiota-derived
components, including but not limited to SCFAs and LPS, may serve as important
effectors responsible of the ENM effect on intestinal immunity. Therefore, the gut
microbiota is implicated as a crucial regulator of the intestinal immunity upon ENM
exposure. This calls for including gut microbiota analysis within future work to
assess ENM biocompatibility and immunosafety. This also calls for refinement of
future studies that should be designed more elaborately and realistically to mimic
the human exposure situation.
AN - rayyan-553781475
AU - Tang, M. X.
AU - Li, S.
AU - Wei, L.
AU - Hou, Z. H.
AU - Qu, J.
AU - Li, L.
DO - 10.3389/fimmu.2021.684605
PY - 2021
SN - 1664-3224
ST - Do Engineered Nanomaterials Affect Immune Responses by Interacting With Gut
Microbiota?
T2 - FRONTIERS IN IMMUNOLOGY
TI - Do Engineered Nanomaterials Affect Immune Responses by Interacting With Gut
Microbiota?
VL - 12
Y2 - 9 y3 - 14
ID - 9415
ER -
TY - JOUR
AB - Bacterial infections have caused many human diseases, so effective treatment
of bacterial infections is an urgent problem that needs to be solved. In this work,
a multifunctional therapeutic core-satellite nanoplatform (ASAN NPs) with
antibacterial and anti-inflammatory properties was designed and synthesized. First,
mesoporous silica-coated silver nanoparticles (Ag NPs) with different sizes were
synthesized via a simplified method that improved the stability and drug-carrying
capacity of the Ag NPs. Then, the NPs were loaded with anti-inflammatory and
antibacterial naringin. The characterization results showed that the ASAN NPs were
successfully synthesized, with an average particle size of 110 ± 15 nm. In vitro
antibacterial test results showed that the synergistic effect of Ag NPs and
naringin enhanced the antibacterial activity in a dose-dependent manner. The ASAN
NPs achieved high-efficiency antibacterial activity by increasing the permeability
of the cell membrane, destroying the integrity of the bacteria, and significantly
increasing the level of bacterial reactive oxygen species. The in vivo
antibacterial results showed that the ASAN NPs had highly effective anti-infective
and anti-inflammatory activities and did not show obvious side effects. Thus, ASAN
NPs appear to be promising antimicrobial agents with good biocompatibility, potent
antibacterial activity, and anti-inflammatory activity. © 2021 Author(s).
AN - rayyan-553781477
AU - Tang, M.
AU - Wang, L.
AU - Zhao, D.
AU - Huang, D.
DO - 10.1063/5.0050866
IS - 5
Biocompatibility
Cytology
Particle size
Silica
Anti-inflammatories
Antibacterial tests
Average particle size
Dose-dependent manner
Flavonoids
PY - 2021
ST - Ag@mSiO2@Ag core-satellite nanostructures enhance the antibacterial and anti-
inflammatory activities of naringin
T2 - AIP Advances
TI - Ag@mSiO2@Ag core-satellite nanostructures enhance the antibacterial and anti-
inflammatory activities of naringin
85106440175&doi=10.1063%2f5.0050866&partnerID=40&md5=433b3447c9dda4a48d9ab6d96ba625
7f
VL - 11
ID - 9416
ER -
TY - JOUR
AB - Silkworm silk is among the most widely used natural fibers for textile and
biomedical applications due to its extraordinary mechanical properties and superior
biocompatibility. A number of physical and chemical processes have also been
developed to reconstruct silk into various forms or to artificially produce silk-
like materials. In addition to the direct use and the delicate replication of
silk's natural structure and properties, there is a growing interest to introduce
more new functionalities into silk while maintaining its advantageous intrinsic
properties. In this review we assess various methods and their merits to produce
functional silk, specifically those with color and luminescence, through post-
processing steps as well as biological approaches. There is a highlight on
intrinsically colored and luminescent silk produced directly from silkworms for a
wide range of applications, and a discussion on the suitable molecular properties
for being incorporated effectively into silk while it is being produced in the silk
gland. With these understanding, a new generation of silk containing various
functional materials (e.g., drugs, antibiotics and stimuli-sensitive dyes) would be
produced for novel applications such as cancer therapy with controlled release
feature, wound dressing with monitoring/sensing feature, tissue engineering
scaffolds with antibacterial, anticoagulant or anti-inflammatory feature, and many
others.
AN - rayyan-553781478
AU - Tansil, N. C.
AU - Koh, L. D.
AU - Han, M. Y.
DO - 10.1002/adma.201104118
IS - 11
KW - Color
PY - 2012
SN - 0935-9648 1521-4095
SP - 1388-1397
ST - Functional Silk: Colored and Luminescent
T2 - ADVANCED MATERIALS
TI - Functional Silk: Colored and Luminescent
VL - 24
Y2 - 3 y3 - 15
ID - 9417
ER -
TY - JOUR
AB - Hospital acquired infections caused due to ESKAPE pathogens pose a challenge
for treatment due to their growing antimicrobial resistance. Curcuma aromatica (CA)
is traditionally known for its antibacterial, wound healing and anti-inflammatory
properties. The present study highlights the biogenic synthesis of silver
nanoparticles (CAAgNPs) capped and stabilized by the compounds from CA rhizome
extract, also further demonstrating their antibacterial, antibiofilm and
synergistic effects against multidrug-resistant (MDR) pathogens. CAAgNPs were
synthesized using aqueous rhizome extract of CA (5 mg/ml) and AgNO3 (0.8 mM)
incubated at 60°C up to 144 h. UV-vis spectroscopy, field emission scanning
electron microscopy (FESEM), transmission electron microscopy (TEM), energy
dispersive spectroscopy (EDS) and X-ray diffraction (XRD) revealed CAAgNPs with
characteristic peak at 430 nm, 13 ± 5 nm size of spherical shape, showing presence
of silver and crystalline nature, respectively. Dynamic light scattering (DLS) and
zeta potential confirmed their monodispersed nature with average diameter of 77.88
± 48.60 nm and stability. Fourier transform infrared spectroscopic (FTIR) analysis
demonstrated the presence of phenolic -OH and carbonyl groups possibly involved in
the reduction and stabilization of CAAgNPs. The minimum inhibitory concentrations
(MICs), minimum bactericidal concentrations (MBCs) and minimum biofilm inhibitory
concentrations (MBICs) of CAAgNPs against Pseudomonas aeruginosa, NCIM 5029 and
PAW1, and, Staphylococcus aureus, NCIM 5021 and S8 were in range from 8 to
128 μg/ml. Almost 50% disruption of pre-formed biofilms at concentrations 8–
1,024 μg/ml was observed. Fluorescence microscopy and FESEM analysis confirmed cell
death and disruption of pre-formed biofilms of P. aeruginosa PAW1 and S. aureus S8.
Checkerboard assay demonstrated the synergistic effect of CAAgNPs (0.125–4 μg/ml)
in combination with various antibiotics (0.063–1,024 μg/ml) against planktonic and
biofilm forms of P. aeruginosa PAW1. The study confirms the antibacterial and
antibiofilm activity of CAAgNPs alone and in combination with antibiotics against
MDR pathogens, thus, reducing the dose as well as toxicity of both. CAAgNPs have
the potential to be used in wound dressings and ointments, and to improve the
performances of medical devices and surgical implants. In vivo toxicity of CAAgNPs
however needs to be tested further using mice models. Copyright © 2022 Tawre,
Shiledar, Satpute, Ahire, Ghosh and Pardesi.
AN - rayyan-553781479
AU - Tawre, M. S.
AU - Shiledar, A.
AU - Satpute, S. K.
AU - Ahire, K.
AU - Ghosh, S.
AU - Pardesi, K.
DO - 10.3389/fchem.2022.1029056
KW - antibiotics
biofilms
Curcuma aromatica
multidrug-resistant
synergy
PY - 2022
ST - Synergistic and antibiofilm potential of Curcuma aromatica derived silver
nanoparticles in combination with antibiotics against multidrug-resistant pathogens
T2 - Frontiers in Chemistry
TI - Synergistic and antibiofilm potential of Curcuma aromatica derived silver
nanoparticles in combination with antibiotics against multidrug-resistant pathogens
85142396300&doi=10.3389%2ffchem.2022.1029056&partnerID=40&md5=082cc50d22f64c87e0d11
5b7f8306f5b
VL - 10
ID - 9418
ER -
TY - JOUR
AB - Silica nanoparticles (SiO2 NPs) are one of the most widely used engineered
nanoparticles and can been found in a wide range of consumer products. Despite
their massive global production scale, little is known about their potential
effects in the context of unintended exposure or ingestion. Using TR146 cells as an
in vitro model of the human oral buccal mucosa, the uptake, spatial intracellular
distribution, reactive oxygen species (ROS) production, inflammatory response, and
cytotoxic effects of commercial SiO2 NPs are examined. SiO2 NPs are shown to dock
and cross the cellular membrane barrier in a dose-time-dependent manner. Confocal
sectioning reveals translocation of SiO2 NPs into the cell nucleus after 12 h of
exposure. A concentration threshold of more than 500 x 10(-6) m is observed, above
which SiO2 NPs are shown to exert significant oxidative stress with concomitant
upregulation of inflammatory genes IL6 and TNFA. Further analysis of the p53
pathway and a series of apoptotic and cell cycle biomarkers reveals intracellular
accumulation of SiO2 NPs exert marginal nanotoxicity. Collectively, this study
provides important information regarding the uptake, intracellular distribution,
and potential adverse cellular effects of SiO2 NPs commonly found in consumer
products in the human oral epithelium.
AN - rayyan-553781480
AU - Tay, C. Y.
AU - Fang, W. R.
AU - Setyawati, M. I.
AU - Sum, C. P.
AU - Xie, J. P.
AU - Ng, K. W.
AU - Chen, X. D.
AU - Hong, C. H. L.
AU - Leong, D. T.
DO - 10.1002/ppsc.201300111
IS - 9
KW - Humanities
Humanism
Humans
Epithelial Cells
PY - 2013
SN - 0934-0866 1521-4117
SP - 784-793
ST - Reciprocal Response of Human Oral Epithelial Cells to Internalized Silica
Nanoparticles
T2 - PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
TI - Reciprocal Response of Human Oral Epithelial Cells to Internalized Silica
Nanoparticles
VL - 30
Y2 - 9
ID - 9419
ER -
TY - JOUR
AB - Polyphenols are a broad group of bioactive phytochemicals with powerful
antioxidant, anti-inflammatory, immunomodulatory, and antiviral activities.
Numerous studies have demonstrated that polyphenol extracts obtained from natural
sources can be used for the prevention and treatment of cancer. Pomegranate peel
extract is an excellent source of polyphenols, such as punicalagin, punicalin,
ellagic acid, and caffeic acid, among others. These phenolic compounds have
antineoplastic activity in in vitro models of cervical cancer through the
regulation of cellular redox balance, induction of apoptosis, cell cycle arrest,
and modulation of different signaling pathways. The current review summarizes
recent data from scientific reports that address the anticancer activity of the
predominant polyphenol compounds present in PPE and their different mechanisms of
action in cervical cancer models.
AN - rayyan-553781481
AU - Teniente, S. L.
AU - Flores-Gallegos, A. C.
AU - Esparza-Gonzalez, S. C.
AU - Campos-Muzquiz, L. G.
AU - Nery-Flores, S. D.
AU - Rodriguez-Herrera, R.
DO - 10.3390/antiox12010127
IS - 1
KW - Uterine Cervical Neoplasms
Polyphenols
PY - 2023
SN - 2076-3921
ST - Anticancer Effect of Pomegranate Peel Polyphenols against Cervical Cancer
T2 - ANTIOXIDANTS
TI - Anticancer Effect of Pomegranate Peel Polyphenols against Cervical Cancer
VL - 12
Y2 - 1
ID - 9420
ER -
TY - JOUR
AB - BACKGROUND: Postconditioning (PostC) with mild hypoxia shortly after a
neonatal hypoxic-ischemic (HI) brain injury can reduce brain damage, however, the
mechanisms underlying this protection are not known. We hypothesize that hypoxic
PostC reduces brain markers of glial activity, inflammation, and apoptosis
following HI injury. METHODS: Sprague Dawley rat pups were exposed to right common
carotid artery occlusion and hypoxia (7% oxygen, 3 h) on postnatal day 7 and 24h
later, pups were exposed to hypoxic PostC (8% O-2 for 1 h/day for 5 d) or kept at
ambient conditions for the same duration. HI+N pups demonstrated similar to 10%
loss in ipsilateral brain tissue which was rescued with HI+PostC.To investigate the
cellular responses, markers of astrocytes, microglia, inflammation, and caspase 3
activity were examined using immunohistochemistry and enzyme-linked immunosorbent
assay. RESULTS: PostC reduced the area of astrocyte staining compared to HI+N.
There was also a shift in microglial morphology toward a primed state in both PostC
groups. Protein levels of interleukin-1 beta and caspase 3 were elevated in HI+N
brains and reduced by PostC. CONCLUSION: This is the first demonstration that PostC
can reduce glial activity, inflammatory mediators, and cell death after a neonatal
HI brain injury.
AN - rayyan-553781482
AU - Teo, J. D.
AU - Morris, M. J.
AU - Jones, N. M.
DO - 10.1038/pr.2015.47
IS - 6
KW - Brain
Rats
Astrocytes
Infant, Newborn
Anoxia
Caspases
PY - 2015
SN - 0031-3998 1530-0447
SP - 757-764
ST - Hypoxic postconditioning reduces microglial activation, astrocyte and caspase
activity, and inflammatory markers after hypoxia-ischemia in the neonatal rat brain
T2 - PEDIATRIC RESEARCH
TI - Hypoxic postconditioning reduces microglial activation, astrocyte and caspase
activity, and inflammatory markers after hypoxia-ischemia in the neonatal rat brain
VL - 77
Y2 - 6
ID - 9421
ER -
TY - JOUR
AB - The Boswellic acids are pentacyclic triterpenoids obtained from the
plant Boswellia serrata gum resin proven to be effective as anti-inflammatory
agents in the treatment of inflammatory bowel disease, rheumatoid arthritis, and
gout. The Boswellic acids were isolated from the gum resin of the plant B.
serrata and characterized by UV-HPLC, TLC, and FTIR studies and further converted
into silver nanoparticles using concentration variation method. The green
synthesized boswellic acid silver nanoparticles were novel structures with 277.3 ±
0.5 nm size, stable without agglomeration characterized by UV-HPLC, DLS, SEM, XRD,
and FTIR spectral studies. The green synthesized boswellic acid silver
nanoparticles were evaluated for in vivo anti-inflammatory activity using the
carrageenan-induced rat paw edema method and also compared with native boswellic
acid and standard diclofenac. The Boswellic acid silver nanoparticles were found to
show high inhibitory activity at 500 mg/kg and 2000 mg/kg body weight dose when
compared to the native boswellic acid and standard diclofenac at the same doses.
The novel Boswellic acid silver nanoparticles were studied for in vitro drug
release kinetics using USP dissolution apparatus I (Basket type) which resulted in
sustained release characteristics when compared with native boswellic acid. The
invivo and invitro correlation for bioavailability and size modulation impact are
needed to establish safety of Boswellic acid silver nanoparticles as novel dosage
forms. © 2022, The Author(s), under exclusive licence to Springer Science+Business
Media, LLC, part of Springer Nature.
AN - rayyan-553781486
AU - Thakur, S.
AU - Mohan, G. K.
DO - 10.1007/s12668-022-00962-6
IS - 2
KW - Boswellic acids
Carrageenan induced rat paw edema method
Green synthesis
In vitro drug release kinetics
Inflammation
Biochemistry
Diseases
Drug products
Kinetics
Rats
Resins
Spectroscopic analysis
acetonitrile
boswellic acid
buffer
carrageenan
diclofenac
distilled water
hexane
methanol
silica gel
silver nanoparticle
silver nitrate
water
Carrageenans
Drug release kinetics
In vitro drug release kinetic
In-vitro
In-vivo
Vitro drug release
acute toxicity
animal experiment
animal model
animal tissue
Article
bioavailability
Boswellia serrata
column chromatography
coma
controlled study
convulsion
diarrhea
drug release
drug safety
lacrimation
lethargy
light dark cycle
mortality
nonhuman
paw edema
physical chemistry
plethysmography
rat
salivation
Soxhlet extraction
thin layer chromatography
tremor
ultrasound
X ray diffraction
Metal nanoparticles
PY - 2022
SP - 670-684
ST - In Vivo Antiinflammatory Activity of Facile Boswellic Acid Silver
Nanoparticles and In Vitro Drug Release Kinetics
T2 - BioNanoScience
TI - In Vivo Antiinflammatory Activity of Facile Boswellic Acid Silver
Nanoparticles and In Vitro Drug Release Kinetics
85127259770&doi=10.1007%2fs12668-022-00962-
6&partnerID=40&md5=8baf038201774f7b92a8447de078daa9
VL - 12
ID - 9425
ER -
TY - JOUR
AB - Silver nanowires (AgNWs) are increasingly being used in the production of
optoelectronic devices, with manufacturing processes posing a risk for occupational
exposures via inhalation. Although some studies have explored the environmental
effects of AgNWs, few data exist on human health effects. Alveolar macrophages are
central in the clearance of inhaled fibers from the lungs, with frustrated
phagocytosis often stated as a key determinant for the onset of inflammatory
reactions. However, the mechanisms through which fully ingested AgNWs interact
with, degrade, and transform within primary macrophages over time, and whether the
reactivity of the AgNWs arises due to ionic or particulate effects, or both, are
poorly understood. Here, a combination of elemental quantification, 3D tomography,
analytical transmission electron microscopy (TEM), and confocal microscopy were
employed to monitor the uptake, intracellular Ag+ availability, and processing of
AgNWs of two different lengths (1 and 10 mu m) inside human monocyte-derived
macrophages (HMMs). Using AgNO3 and spherical silver nanoparticles (AgNPs) as a
comparison, the amount of total bioavailable/intracellular Ag highly correlated to
the cytotoxicity of AgNWs. The 10 mu m AgNWs were completely internalized in HMMs,
with numerous lysosomal vesicles observed in close vicinity to the AgNWs. Following
cellular uptake, AgNWs dissolved and transformed intracellularly, with
precipitation of AgCI as well as Ag2S. These transformation processes were likely
due to AgNW degradation in the acidic environment of lysosomes, leading to the
release of Ag+ ions that rapidly react with Cl- and SH- species of the cell
microenvironment. Our data suggest that, in HMMs, not only frustrated phagocytosis
but also the extent of intracellular uptake and dissolution of AgN'vVs dictates
their cytotoxicity.
AN - rayyan-553781488
AU - Theodorou, I. G.
AU - Muller, K. H.
AU - Chen, S.
AU - Goode, A. E.
AU - Yufit, V.
AU - Ryan, M. P.
AU - Porter, A. E.
IS - 10
KW - Humanities
Humanism
Humans
Macrophages
Monocytes
Nanowires
PY - 2017
SN - 2373-9878
SP - 2336-2347
ST - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
Cells: Intracellular Availability of Silver Governs Their Cytotoxicity
T2 - ACS BIOMATERIALS SCIENCE & ENGINEERING
TI - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
VL - 3
Y2 - 10
ID - 9427
ER -
TY - JOUR
AB - Silver nanowires (AgNWs) are increasingly being used in the production of
optoelectronic devices, with manufacturing processes posing a risk for occupational
exposures via inhalation. Although some studies have explored the environmental
effects of AgNWs, few data exist on human health effects. Alveolar macrophages are
central in the clearance of inhaled fibers from the lungs, with frustrated
phagocytosis often stated as a key determinant for the onset of inflammatory
reactions. However, the mechanisms through which fully ingested AgNWs interact
with, degrade, and transform within primary macrophages over time, and whether the
reactivity of the AgNWs arises due to ionic or particulate effects, or both, are
poorly understood. Here, a combination of elemental quantification, 3D tomography,
analytical transmission electron microscopy (TEM), and confocal microscopy were
employed to monitor the uptake, intracellular Ag(+) availability, and processing of
AgNWs of two different lengths (1 and 10 μm) inside human monocyte-derived
macrophages (HMMs). Using AgNO(3) and spherical silver nanoparticles (AgNPs) as a
comparison, the amount of total bioavailable/intracellular Ag highly correlated to
the cytotoxicity of AgNWs. The 10 μm AgNWs were completely internalized in HMMs,
with numerous lysosomal vesicles observed in close vicinity to the AgNWs. Following
cellular uptake, AgNWs dissolved and transformed intracellularly, with
precipitation of AgCl as well as Ag(2)S. These transformation processes were likely
due to AgNW degradation in the acidic environment of lysosomes, leading to the
release of Ag(+) ions that rapidly react with Cl(-) and SH(-) species of the cell
microenvironment. Our data suggest that, in HMMs, not only frustrated phagocytosis
but also the extent of intracellular uptake and dissolution of AgNWs dictates their
cytotoxicity.
AN - rayyan-553782267
AU - Theodorou, I. G.
AU - Müller, K. H.
AU - Chen, S.
AU - Goode, A. E.
AU - Yufit, V.
AU - Ryan, M. P.
AU - Porter, A. E.
IS - 10
J2 - ACS Biomater Sci Eng
KW - Humanities
Humanism
Humans
Macrophages
Monocytes
Nanowires
LA - eng
N1 - Department of Materials and London Centre for Nanotechnology, Imperial
College London, Exhibition Road, London SW7 2AZ, United Kingdom.; Cambridge
Advanced Imaging Centre, Department of Physiology, Development and Neuroscience,
University of Cambridge, Downing Street, Cambridge CB2 3DY, United Kingdom.;
Department of Biological Sciences and Institute of Structural and Molecular Biology
(ISMB), Birkbeck College, University of London, Malet Street, London, WC1E 7HX,
United Kingdom.; Department of Materials and London Centre for Nanotechnology,
Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.;
Department of Earth Science & Engineering, Imperial College London, Exhibition
Road, London SW7 2AZ, United Kingdom.; Department of Materials and London Centre
for Nanotechnology, Imperial College London, Exhibition Road, London SW7 2AZ,
United Kingdom.; Department of Materials and London Centre for Nanotechnology,
Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.
PY - 2017
SP - 2336-2347
ST - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
T2 - ACS biomaterials science & engineering
TI - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
VL - 3
Y2 - 10 y3 - 9
ID - 10177
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials.
Following oral exposure, AgNPs can accumulate in various organs including kidneys
where they show gender specific accumulation. There is limited information on their
effect on renal system following long-term animal exposure especially at the
ultramicroscopic and molecular level. In this study, we have assessed the effect of
60 days oral AgNPs treatment on kidneys of female Wistar rats at doses of 50 ppm
and 200 ppm that are below previously reported lowest observed adverse effect level
(LOAEL). AgNPs treatment led to decrease in kidney weight and some loss of renal
function as seen by increased levels of serum creatinine and early toxicity markers
such as KIM-1, clusterin and osteopontin. We also observed significant
mitochondrial damage, loss of brush border membranes, pronounced swelling of
podocytes and degeneration of their foot processes using transmission electron
microscopy (TEM). These symptoms are similar to those seen in nephrotic syndrome
and ‘Minimal change disease’ of kidney where few changes are visible under light
microscopy but significant ultrastructural damage is observed. Prolonged treatment
of AgNPs also led to the activation of cell proliferative, survival and
proinflammatory factors (Akt/mTOR, JNK/Stat and Erk/NF-κB pathways and IL1β, MIP2,
IFN-γ, TNF-α and RANTES) and dysfunction of normal apoptotic pathway. Our study
shows how long term AgNPs exposure may promote ultrastructural damage to kidney
causing inflammation and expression of cell survival factors. These changes, in the
long term, could lead to inhibition of the beneficial apoptotic pathway and
promotion of necrotic cell death in kidneys. © 2017 Informa UK Limited, trading as
AN - rayyan-553781491
AU - Tiwari, R.
AU - Singh, R. D.
AU - Khan, H.
AU - Gangopadhyay, S.
AU - Mittal, S.
AU - Singh, V.
AU - Arjaria, N.
AU - Shankar, J.
AU - Roy, S. K.
AU - Singh, D.
AU - Srivastava, V.
DO - 10.1080/17435390.2017.1343874
IS - 5
KW - growth factor signaling
inflammation
kidney
Nanosilver
necrosis
nephrotoxicity
Animals
Apoptosis
Female
Kidney
Metal Nanoparticles
Necrosis
Rats
Rats, Wistar
Silver
Toxicity Tests, Subchronic
clusterin
CXCL3 chemokine
gamma interferon
interleukin 12p40
interleukin 17
interleukin 18
interleukin 1alpha
interleukin 1beta
interleukin 6
interleukin 7
kidney injury molecule 1
mammalian target of rapamycin
osteopontin
protein kinase B
RANTES
silver nanoparticle
STAT protein
stress activated protein kinase
tissue inhibitor of metalloproteinase 1
vasculotropin
metal nanoparticle
silver
animal experiment
animal model
animal tissue
apoptosis
Article
brush border
cell death
cell proliferation
cell survival
cell swelling
cell ultrastructure
controlled study
DNA damage
female
glomerulus basement membrane
kidney dysfunction
kidney injury
kidney mass
long term exposure
microscopy
minimal change glomerulonephritis
mitochondrial toxicity
nanotoxicology
nephrotic syndrome
nonhuman
oxidative stress
podocyte
priority journal
protein expression
rat
Wistar rat
animal
chemically induced
cytology
drug effects
pathology
pathophysiology
toxicity testing
PY - 2017
SP - 671-686
ST - Oral subchronic exposure to silver nanoparticles causes renal damage through
apoptotic impairment and necrotic cell death
T2 - Nanotoxicology
TI - Oral subchronic exposure to silver nanoparticles causes renal damage through
apoptotic impairment and necrotic cell death
85021803129&doi=10.1080%2f17435390.2017.1343874&partnerID=40&md5=a5195a7e97a86f18a1
e290b592f35df4
VL - 11
ID - 9430
ER -
TY - JOUR
AB - Chronic, multifactorial illnesses of the gastrointestinal tract include
inflammatory bowel diseases. One of the greatest methods for regulated medicine
administration in a particular region of inflammation is the nanoparticle system.
Silver nanoparticles (Ag NPs) have been utilized as drug delivery systems in the
pharmaceutical industry. The goal of the current study is to synthesize drug-loaded
Ag NPs using a previously described 3-methyl-1-phenylbutan-2-amine, as a mebeverine
precursor (MP). Methods: A green, galactose-assisted method for the rapid synthesis
and stabilization of Ag NPs as a drug-delivery system is presented. Galactose was
used as a reducing and capping agent forming a thin layer encasing the
nanoparticles. Results: The structure, size distribution, zeta potential, surface
charge, and the role of the capping agent of drug-loaded Ag NPs were discussed. The
drug release of the MP-loaded Ag NPs was also investigated. The Ag NPs indicated a
very good drug release between 80 and 85%. Based on the preliminary results, Ag NPs
might be a promising medication delivery system for MP and a useful treatment
option for inflammatory bowel disease. Therefore, future research into the
potential medical applications of the produced Ag NPs is necessary.
AN - rayyan-553781492
AU - Todorova, M.
AU - Milusheva, M.
AU - Kaynarova, L.
AU - Georgieva, D.
AU - Delchev, V.
AU - Simeonova, S.
AU - Pilicheva, B.
AU - Nikolova, S.
IS - 6
KW - Inflammatory Bowel Diseases
PY - 2023
SN - 2227-9059
ST - Drug-Loaded Silver Nanoparticles-A Tool for Delivery of a Mebeverine
Precursor in Inflammatory Bowel Diseases Treatment
T2 - BIOMEDICINES
TI - Drug-Loaded Silver Nanoparticles-A Tool for Delivery of a Mebeverine
Precursor in Inflammatory Bowel Diseases Treatment
VL - 11
Y2 - 6
ID - 9431
ER -
TY - JOUR
AB - This work determines whether cytokine-induced neutrophil chemoattractants
(CINC)-1, CINC-2 and CINC-3 can be markers for predicting high or low pulmonary
toxicity of nanomaterials (NMs). We classified NMs of nickel oxide (NiO) and cerium
dioxide (CeO2) into high toxicity and NMs of two types of titanium dioxides
(TiO2(P90 and rutile)) and zinc oxide (ZnO) into low toxicity, and we analyzed
previous data of CINCs in bronchoalveolar lavage fluid (BALF) of rats from three
days to six months after intratracheal instillation (0.2 and 1.0 mg) and inhalation
exposure (0.32-10.4 mg/m(3)) of materials (NiO, CeO2, TiO2(P90 and rutile), ZnO NMs
and micron-particles of crystalline silica (SiO2)). The concentration of CINC-1 and
CINC-2 in BALF had different increase tendency between high and low pulmonary
toxicity of NMs and correlated with the other inflammatory markers in BALF.
However, CINC-3 increased only slightly in a dose-dependent manner compared with
CINC-1 and CINC-2. Analysis of receiver operating characteristics for the toxicity
of NMs by CINC-1 and CINC-2 showed the most accuracy of discrimination of the
toxicity at one week or one month after exposure and CINC-1 and CINC-2 in BALF
following intratracheal instillation of SiO(2)as a high toxicity could accurately
predict the toxicity at more than one month after exposure. These data suggest that
CINC-1 and CINC-2 may be useful biomarkers for the prediction of pulmonary toxicity
of NMs relatively early in both intratracheal instillation and inhalation exposure.
AN - rayyan-553781494
AU - Tomonaga, T.
AU - Izumi, H.
AU - Oyabu, T.
AU - Lee, B. W.
AU - Kubo, M.
AU - Shimada, M.
AU - Noguchi, S.
AU - Nishida, C.
AU - Yatera, K.
AU - Morimoto, Y.
DO - 10.3390/nano10081563
IS - 8
KW - Cytokines
Biological Markers
PY - 2020
SN - 2079-4991
ST - Assessment of Cytokine-Induced Neutrophil Chemoattractants as Biomarkers for
Prediction of Pulmonary Toxicity of Nanomaterials
T2 - NANOMATERIALS
TI - Assessment of Cytokine-Induced Neutrophil Chemoattractants as Biomarkers for
Prediction of Pulmonary Toxicity of Nanomaterials
VL - 10
Y2 - 8
ID - 9433
ER -
TY - JOUR
AB - Purpose: It was aimed to investigate the biochemical and immunohistochemical
effects of ephedrine (EPH) in bilateral ovariectomized rats. Methods: Twenty-four
Sprague Dawley female rats were divided into three groups: control group: The
abdomen was opened and closed without any treatment; ischemia-reperfusion (IR)
group: 2 h of ischemia followed by 2 h of reperfusion were allowed to cause IR
injury; IR+EPH group: oral EPH solution (5 mg/kg) was administered for 28 days.
Results: Biochemical parameters were statistically significant in group
comparisons. Increased interleukin-6 (IL-6) expression, degenerative preantral and
antral follicle cells and inflammatory cells around blood vessels were seen in IR
group. Negative IL-6 expression was observed in seminal epithelial cells, preantral
and antral follicle cells in IR+EPH group. While caspase-3 activity increased in
granulosa cells and stromal cells in IR group, caspase-3 expression was negative in
preantral and antral follicle cells in the germinal epithelium and cortex in IR+EPH
group. Conclusion: The effect of apoptosis, which occurs with the signaling that
starts in the cell nucleus, caused the cessation of the stimulating effect at the
nuclear level after EPH administration, and a decrease in the antioxidative effect
in IR damage and inflammation in the apoptotic process.
AN - rayyan-553781497
AU - Toprak, Veysel
AU - Akalin, Senem Alkan
AU - Öcal, Ece
AU - Çavus, Yunus
AU - Deveci, Engin
DO - 10.1590/acb381523
KW - Antioxidants
Apoptosis
Caspase 3
Interleukin-6
Ovary
Ephedrine
Rats
LA - en
PY - 2023
SN - 0102-8650
SP - e381523-e381523
ST - Biochemical and immunohistochemical examination of the effects of ephedrine
in rat ovary tissue
T2 - Acta cir. bras
TI - Biochemical and immunohistochemical examination of the effects of ephedrine
in rat ovary tissue
86502023000100212
VL - 38
ID - 9436
ER -
TY - JOUR
AB - Synthetic amorphous silica is one of the most used nanomaterials, and
numerous toxicological studies have studied its effects. Most of these studies have
used an acute exposure mode to investigate the effects immediately after exposure.
However, this exposure modality does not allow the investigation of the persistence
of the effects, which is a crucial aspect of silica toxicology, as exemplified by
crystalline silica. In this paper, we extended the investigations by studying not
only the responses immediately after exposure but also after a 72 h post-exposure
recovery phase. We used a pyrolytic silica as the test nanomaterial, as this
variant of synthetic amorphous silica has been shown to induce a more persistent
inflammation in vivo than precipitated silica. To investigate macrophage responses
to pyrolytic silica, we used a combination of proteomics and targeted experiments,
which allowed us to show that most of the cellular functions that were altered
immediately after exposure to pyrolytic silica at a subtoxic dose, such as energy
metabolism and cell morphology, returned to normal at the end of the recovery
period. However, some alterations, such as the inflammatory responses and some
aldehyde detoxification proteins, were persistent. At the proteomic level, other
alterations, such as proteins implicated in the endosomal/lysosomal pathway, were
also persistent but resulted in normal function, thus suggesting cellular
adaptation.
AN - rayyan-553781499
AU - Torres, A.
AU - Dalzon, B.
AU - Diemer, H.
AU - Fenel, D.
AU - Schoehn, G.
AU - Cianferani, S.
AU - Carriere, M.
AU - Rabilloud, T.
DO - 10.3390/nano10101939
IS - 10
KW - Macrophages
Proteomics
Proteome
PY - 2020
SN - 2079-4991
ST - How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-
Informed View
T2 - NANOMATERIALS
TI - How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-
Informed View
VL - 10
Y2 - 10
ID - 9438
ER -
TY - JOUR
AB - CD47 is an antiphagocytic "don't eat me" signal that inhibits programmed cell
removal of self. As red blood cells (RBCs) age they lose CD47 expression and become
susceptible to programmed cell removal by macrophages. CD47(-/-) mice infected with
Plasmodium yoelii, which exhibits an age-based preference for young RBCs, were
previously demonstrated to be highly resistant to malaria infection. Our study
sought to test the therapeutic benefit of CD47 blockade on ameliorating the
clinical syndromes of experimental cerebral malaria (ECM), using the Plasmodium
berghei ANKA (Pb-A) murine model. In vitro we tested the effect of anti-CD47 mAb on
Plasmodium-infected RBC phagocytosis and found that anti-CD47 treatment
significantly increased clearance of Plasmodium-infected RBCs. Infection of C57BL/6
mice with Pb-A is lethal and mice succumb to the clinical syndromes of CM between
days 6 and 10 postinfection. Strikingly, treatment with anti-CD47 resulted in
increased survival during the cerebral phase of Pb-A infection. Anti-CD47-treated
mice had increased lymphocyte counts in the peripheral blood and increased
circulating levels of IFN-γ, TNF-α, and IL-22. Despite increased circulating levels
of inflammatory cytokines, anti-CD47-treated mice had reduced pathological features
in the brain. Survival of ECM in anti-CD47-treated mice was correlated with reduced
cellular accumulation in the cerebral vasculature, improved blood-brain barrier
integrity, and reduced cytotoxic activity of infiltrating CD8(+) T cells. These
results demonstrate the therapeutic benefit of anti-CD47 to reduce morbidity in a
lethal model of ECM, which may have implications for preventing mortality in young
African children who are the highest casualties of CM.
AN - rayyan-553782403
AU - Torrez Dulgeroff, L. B.
AU - Oakley, M. S.
AU - Tal, M. C.
AU - Yiu, Y. Y.
AU - He, J. Q.
AU - Shoham, M.
AU - Majam, V.
AU - Okoth, W. A.
AU - Malla, P.
AU - Kumar, S.
AU - Weissman, I. L.
DO - 10.1073/pnas.1907653118
IS - 11
J2 - Proc Natl Acad Sci U S A
KW - Animals
Antibodies, Monoclonal/immunology
CD47 Antigen/*antagonists & inhibitors/immunology
Erythrocytes/parasitology
*Host-Parasite Interactions
Humans
Malaria, Cerebral/*pathology/prevention & control
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Phagocytosis
Malaria
Plasmodium
Malaria, Cerebral
LA - eng
N1 - Institute for Stem Cell Biology and Regenerative Medicine, Stanford
University School of Medicine, Stanford, CA 94305; laughingbeartorrez@gmail.com
irv@stanford.edu.; Laboratory of Emerging Pathogens, Division of Emerging and
Transfusion Transmitted Diseases, Office of Blood Research and Review, Center for
Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD
20993.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford
University School of Medicine, Stanford, CA 94305.; Institute for Stem Cell Biology
and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA
94305.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford
University School of Medicine, Stanford, CA 94305.; Institute for Stem Cell Biology
and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA
94305.; Laboratory of Emerging Pathogens, Division of Emerging and Transfusion
Transmitted Diseases, Office of Blood Research and Review, Center for Biologics
Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993.;
Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted
Diseases, Office of Blood Research and Review, Center for Biologics Evaluation and
Research, Food and Drug Administration, Silver Spring, MD 20993.; Laboratory of
Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases,
Office of Blood Research and Review, Center for Biologics Evaluation and Research,
Food and Drug Administration, Silver Spring, MD 20993.; Laboratory of Emerging
Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Office of
Blood Research and Review, Center for Biologics Evaluation and Research, Food and
Drug Administration, Silver Spring, MD 20993.; Institute for Stem Cell Biology and
Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305;
laughingbeartorrez@gmail.com irv@stanford.edu.
PY - 2021
ST - CD47 blockade reduces the pathologic features of experimental cerebral
malaria and promotes survival of hosts with Plasmodium infection
T2 - Proceedings of the National Academy of Sciences of the United States of
America
TI - CD47 blockade reduces the pathologic features of experimental cerebral
malaria and promotes survival of hosts with Plasmodium infection
VL - 118
Y2 - 3 y3 - 16
ID - 10310
ER -
TY - JOUR
AB - Lung fibrosis involves the overexpression of ECM proteins, primarily
collagen, by alpha-smooth muscle actin (ASMA)-positive cells. Caveolin-1 is a
master regulator of collagen expression by cultured lung fibroblasts and of lung
fibrosis in vivo. A peptide equivalent to the caveolin-1 scaffolding domain (CSD
peptide) inhibits collagen and tenascin-C expression by normal lung fibroblasts
(NLF) and fibroblasts from the fibrotic lungs of scleroderma patients (SLF). CSD
peptide inhibits ASMA expression in SLF but not NLF. Similar inhibition of
collagen, tenascin-C, and ASMA expression was also observed when caveolin-1
expression was upregulated using adenovirus. These observations suggest that the
low caveolin-1 levels in SLF cause their overexpression of collagen, tenascin-C,
and ASMA. In mechanistic studies, MEK, ERK, JNK, and Akt were hyperactivated in
SLF, and CSD peptide inhibited their activation and altered their subcellular
localization. These studies and experiments using kinase inhibitors suggest many
differences between NLF and SLF in signaling cascades. To validate these data, we
determined that the alterations in signaling molecule activation observed in SLF
also occur in fibrotic lung tissue from scleroderma patients and in mice with
bleomycin-induced lung fibrosis. Finally, we demonstrated that systemic
administration of CSD peptide to bleomycin-treated mice blocks epithelial cell
apoptosis, inflammatory cell infiltration, and changes in tissue morphology as well
as signaling molecule activation and collagen, tenascin-C, and ASMA expression
associated with lung fibrosis. CSD peptide may be a prototype for novel treatments
for human lung fibrosis that act, in part, by inhibiting the expression of ASMA and
ECM proteins.
AN - rayyan-553781500
AU - Tourkina, E.
AU - Richard, M.
AU - Gooz, P.
AU - Bonner, M.
AU - Pannu, J.
AU - Harley, R.
AU - Bernatchez, P. N.
AU - Sessa, W. C.
AU - Silver, R. M.
AU - Hoffman, S.
DO - 10.1152/ajplung.00295.2007
IS - 5
PY - 2008
SN - 1040-0605 1522-1504
SP - L843-L861
ST - Antifibrotic properties of caveolin-1 scaffolding domain in vitro and in vivo
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
TI - Antifibrotic properties of caveolin-1 scaffolding domain in vitro and in vivo
VL - 294
Y2 - 5
ID - 9439
ER -
TY - JOUR
AB - Since no approved therapies to restore mobility and sensation following
spinal cord injury (SCI) currently exist, a better understanding of the cellular
and molecular mechanisms following SCI that compromise regeneration or
neuroplasticity is needed to develop new strategies to promote axonal regrowth and
restore function. Physical trauma to the spinal cord results in vascular disruption
that, in turn, causes blood-spinal cord barrier rupture leading to hemorrhage and
ischemia, followed by rampant local cell death. As subsequent edema and
inflammation occur, neuronal and glial necrosis and apoptosis spread well beyond
the initial site of impact, ultimately resolving into a cavity surrounded by
glial/fibrotic scarring. The glial scar, which stabilizes the spread of secondary
injury, also acts as a chronic, physical, and chemo-entrapping barrier that
prevents axonal regeneration. Understanding the formative events in glial scarring
helps guide strategies towards the development of potential therapies to enhance
axon regeneration and functional recovery at both acute and chronic stages
following SCI. This review will also discuss the perineuronal net and how
chondroitin sulfate proteoglycans (CSPGs) deposited in both the glial scar and net
impede axonal outgrowth at the level of the growth cone. We will end the review
with a summary of current CSPG-targeting strategies that help to foster axonal
regeneration, neuroplasticity/sprouting, and functional recovery following SCI.
AN - rayyan-553781502
AU - Tran, A. P.
AU - Warren, P. M.
AU - Silver, J.
DO - 10.1152/physrev.00017.2017
IS - 2
KW - Spinal Cord Injuries
Spinal Cord
Regeneration
PY - 2018
SN - 0031-9333 1522-1210
SP - 881-917
ST - THE BIOLOGY OF REGENERATION FAILURE AND SUCCESS AFTER SPINAL CORD INJURY
T2 - PHYSIOLOGICAL REVIEWS
TI - THE BIOLOGY OF REGENERATION FAILURE AND SUCCESS AFTER SPINAL CORD INJURY
VL - 98
Y2 - 4
ID - 9441
ER -
TY - JOUR
AB - Using analytical chemistry techniques such as nuclear magnetic resonance
(NMR) spectroscopy and liquid or gas chromatography–mass spectrometry (LC/GC-MS),
metabolomics allows detection of most endogenous and exogenous metabolites in a
biological sample. Metabolomics has a wide range of applications, and has been
employed in nutrition science, toxicology, environmental studies, and systems
biology. Metabolomics is particularly useful in biomedical science, and has been
used for diagnostic laboratory testing, identifying targets for drug development,
and monitoring drug metabolism, mode of action, and toxicity. Despite its immense
potential, metabolomics remains underutilized in the study of spontaneous animal
diseases. Our aim was to comprehensively review the existing literature on the use
of metabolomics in spontaneous veterinary diseases. Three databases were used to
find journal articles that applied metabolomics in veterinary medicine. A screening
process was then conducted to eliminate references that did not meet the
eligibility criteria; only primary research studies investigating spontaneous
animal disease were included; 38 studies met the inclusion criteria. The main
techniques used were NMR and MS. All studies detected metabolite alterations in
diseased animals compared with non-diseased animals. Metabolomics was mainly used
to study diseases of the digestive, reproductive, and musculoskeletal systems.
Inflammatory conditions made up the largest proportion of studies when articles
were categorized by disease process. Following a comprehensive analysis of the
literature on metabolomics in spontaneous veterinary diseases, we concluded that
metabolomics, although in its early stages in veterinary research, is a promising
tool regarding diagnosis, biomarker discovery, and in uncovering new insights into
disease pathophysiology. © 2020 The Author(s).
AN - rayyan-553781503
AU - Tran, H.
AU - McConville, M.
AU - Loukopoulos, P.
DO - 10.1177/1040638720948505
IS - 5
KW - metabolomics
omics
oncology
one health
review
veterinary
Animal Diseases
Animals
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Metabolomics
alanine
biological marker
carnosine
choline
creatinine
fumaric acid
gluconic acid
glutamic acid
glutamine
glycylproline
hexuronic acid
hydroxybutyric acid
isoleucine
lactone
lysine
lysophosphatidylcholine
methylamine
n acetylaspartic acid
nicotinamide phosphoribosyltransferase
palmitoleic acid
phosphorylcholine
propionic acid
pyruvic acid
serum amyloid A
silver nanoparticle
taurine
tryptophan
tyrosine
unindexed drug
animal disease
anxiety
biopsy
Bovine coronavirus
cat
cell culture
cerebrospinal fluid
citric acid cycle
cow
diabetes mellitus
diarrhea
dog
doping
drug metabolism
fever
fourier transform ion cyclotron resonance mass spectrometry
horse
isotope labeling
ketoacidosis
laboratory test
liver tissue
lymphoma
metabolite
muscular dystrophy
newborn sepsis
nonhuman
nutrition
osteoarthritis
osteochondrosis
proteomics
Review
sheep
systems biology
temperature stress
toxicology
transitional cell carcinoma
ultra performance liquid chromatography
vascular disease
veterinary medicine
animal
metabolism
procedures
Animal Shells
PY - 2020
SP - 635-647
ST - Metabolomics in the study of spontaneous animal diseases
T2 - Journal of Veterinary Diagnostic Investigation
TI - Metabolomics in the study of spontaneous animal diseases
85089524445&doi=10.1177%2f1040638720948505&partnerID=40&md5=8ebe7bdb4404925c2ae490f
1be0e6d40
VL - 32
ID - 9442
ER -
TY - JOUR
AB - Psoriasis is a common T-cell-mediated immune disorder characterized by
circumscribed, red, thickened plaques with an overlying silver-white scale. It
occurs worldwide, although the incidence is lower in warmer, sunnier climates. The
primary cause of psoriasis is unknown. During an active disease state, an
underlying inflammatory mechanism is frequently involved. Many conventional
treatments focus on suppressing symptoms associated with psoriasis and have
significant side effects. This article reviews several of the researched natural
approaches to psoriasis treatment, while addressing its underlying cause.
AN - rayyan-553781504
AU - Traub, M.
AU - Marshall, K.
IS - 4
KW - Complementary Therapies
Diet Therapy
Dietary Supplements
Humans
Immunity, Cellular
Immunosuppressive Agents
Life Style
Psoriasis
Risk Factors
adalimumab
alefacept
Berberis aquifolium extract
calcipotriol
CD4 antigen
CD8 antigen
cerave
corticosteroid
curcumin
cyclosporin
dithranol
efalizumab
etanercept
flavsalve
folic acid
fumaric acid
gamma interferon
herose
infliximab
interleukin 2
leukotriene B4
leukotriene B5
methotrexate
mimyxaveeno eczema care
placebo
plant extract
prostaglandin E1
prostaglandin E2
prostaglandin E3
psoriaflora
relieva
tar
tazarotene
thromboxane A2
unclassified drug
unindexed drug
xp 828l
alternative medicine
CD4+ T lymphocyte
CD8+ T lymphocyte
clinical feature
clinical trial
dendritic cell
diet therapy
disease severity
drug indication
fatigue
folic acid deficiency
gastrointestinal symptom
headache
human
kidney injury
lifestyle modification
liver fibrosis
liver toxicity
megaloblastic anemia
nausea
pathophysiology
phototherapy
prevalence
psoriasis
PUVA
rebound
review
risk factor
side effect
sun exposure
symptom
Th1 cell
treatment indication
tropic climate
PY - 2007
SP - 319-330
ST - Psoriasis - Pathophysiology, conventional, and alternative approaches to
treatment
T2 - Alternative Medicine Review
TI - Psoriasis - Pathophysiology, conventional, and alternative approaches to
treatment
37549028062&partnerID=40&md5=2adb4821745139c33fbd22bf00f9d334
VL - 12
ID - 9443
ER -
TY - JOUR
AB - The molecular responses of macrophages to copper-based nanoparticles have
been investigated via a combination of proteomic and biochemical approaches, using
the RAW264.7 cell line as a model. Both metallic copper and copper oxide
nanoparticles have been tested, with copper ion and zirconium oxide nanoparticles
used as controls. Proteomic analysis highlighted changes in proteins implicated in
oxidative stress responses (superoxide dismutases and peroxiredoxins), glutathione
biosynthesis, the actomyosin cytoskeleton, and mitochondrial proteins (especially
oxidative phosphorylation complex subunits). Validation studies employing
functional analyses showed that the increases in glutathione biosynthesis and in
mitochondrial complexes observed in the proteomic screen were critical to cell
survival upon stress with copper-based nanoparticles; pharmacological inhibition of
these two pathways enhanced cell vulnerability to copper-based nanoparticles, but
not to copper ions. Furthermore, functional analyses using primary macrophages
derived from bone marrow showed a decrease in reduced glutathione levels, a
decrease in the mitochondrial transmembrane potential, and inhibition of
phagocytosis and of lipopolysaccharide-induced nitric oxide production. However,
only a fraction of these effects could be obtained with copper ions. In conclusion,
this study showed that macrophage functions are significantly altered by copper-
based nanoparticles. Also highlighted are the cellular pathways modulated by cells
for survival and the exemplified cross-toxicities that can occur between copper-
based nanoparticles and pharmacological agents. Molecular & Cellular Proteomics 12:
10.1074/mcp.M113.030742, 3108-3122, 2013.
AN - rayyan-553781505
AU - Triboulet, S.
AU - Carriere, M.
AU - Diemer, H.
AU - Proamer, F.
AU - Habert, A.
AU - Chevallet, M.
AU - Strub, J. M.
AU - Hanau, D.
AU - Van Dorsselaer, A.
AU - Rabilloud, T.
DO - 10.1074/mcp.M113.030742
IS - 11
KW - Mice
Macrophages
Proteomics
Copper
Proteome
PY - 2013
SN - 1535-9476 1535-9484
SP - 3108-3122
ST - Molecular Responses of Mouse Macrophages to Copper and Copper Oxide
Nanoparticles Inferred from Proteomic Analyses
T2 - MOLECULAR & CELLULAR PROTEOMICS
TI - Molecular Responses of Mouse Macrophages to Copper and Copper Oxide
Nanoparticles Inferred from Proteomic Analyses
VL - 12
Y2 - 11
ID - 9444
ER -
TY - JOUR
AB - To reduce the incidence of implant-associated infection, we previously
developed a novel coating technology using hydroxyapatite (HA) containing silver
(Ag). This study examined in vivo acute and subacute toxicity associated with the
Ag-HA coating in rat tibiae. Ten-week-old rats received implantation of HA-, 2% Ag-
HA-, or 50% Ag-HA-coated titanium rods. Concentrations of silver in serum, brain,
liver, kidneys, and spleen were measured in the acute phase (2-4 days after
treatment) and subacute phase (4-12 weeks after treatment). Biochemical and
histological examinations of those organs were also performed. Mean serum silver
concentration peaked in the acute phase and then gradually decreased. Mean silver
concentrations in all examined organs from the 2% Ag-HA coating groups showed no
significant differences compared with the HA coating group. No significant
differences in mean levels of glutamic-oxaloacetic transaminase, glutamic-pyruvic
transaminase, lactate dehydrogenase, creatinine, or blood urea nitrogen were seen
between the three groups and controls. Histological examinations of all organs
revealed no abnormal pathologic findings. No acute or subacute toxicity was seen in
vivo for 2% Ag-HA coating or HA coating. Ag-HA coatings on implants may represent
biologically safe antibacterial biomaterials and may be of value for reducing
surgical-site infections related to implantation. © 2014 Masatsugu Tsukamoto et al.
AN - rayyan-553781507
AU - Tsukamoto, M.
AU - Miyamoto, H.
AU - Ando, Y.
AU - Noda, I.
AU - Eto, S.
AU - Akiyama, T.
AU - Yonekura, Y.
AU - Sonohata, M.
AU - Mawatari, M.
DO - 10.1155/2014/902343
KW - Animals
Durapatite
Rats
Silver
Tibia
Titanium
creatinine
hydroxyapatite
nitrogen
silver
titanium
urea
antiinfective agent
biomaterial
animal experiment
animal tissue
article
body weight
brain
controlled study
foreign body granuloma
histopathology
in vivo study
kidney
liver
male
nonhuman
orthopedic implant
prosthesis infection
rat
spleen
tibia
animal
pathology
surgery
PY - 2014
ST - Acute and subacute toxicity in vivo of thermal-sprayed silver containing
hydroxyapatite coating in rat tibia
T2 - BioMed Research International
TI - Acute and subacute toxicity in vivo of thermal-sprayed silver containing
hydroxyapatite coating in rat tibia
84897497385&doi=10.1155%2f2014%2f902343&partnerID=40&md5=319fc3b10f34a4cdc4c8dfd914
60d6c1
VL - 2014
ID - 9446
ER -
TY - JOUR
AB - Nowadays, the unique features of nanoparticles (NPs) have encouraged new
applications in different areas including biology, medicine, agriculture, and
electronics. Their quick joining into daily life not only enhances the uses of NPs
in a wide range of modern technologies but also their release into the aquatic
environment causes inevitable environmental concerns. On the other hand boron
exhibits key physiological effects on biological systems. This research was
designed for evaluating the toxicity of magnetite nanoparticles (Fe3O4-MNPs) on
aquatic organisms and obtaining data for the information gap in this area. In this
study, Rainbow trout (Oncorhynchus mykiss) was considered as an aquatic indicator,
and trials were designed as Ulexite (a boron mineral, UX) treatment against
exposure to Fe3O4-MNPs. Synthesized and characterized Fe3O4-MNPs were exposed to
rainbow trouts in wide spectrum concentrations (0.005-0.08 mL/L) to analyze its
lethal dose (LC50) and cytoprotective properties by UX treatment were assessed
against Fe3O4-MNPs applications for 96 h. For the initial toxicity analysis,
hematological parameters (blood cell counts) were examined in experimental groups
and micronucleus (MN) assay was performed to monitor nuclear abnormalities after
exposure to NPs. Biochemical analyzes in both blood and liver samples were utilized
to assess antioxidant/oxidative stress and inflammatory parameters. Also, 8-
hydroxy-2'-deoxyguanosine (8-OHdG) assay was used to investigate oxidative DNA
lesions and Caspase-3 analysis was performed on both blood and liver tissues to
monitor apoptotic cell death occurrence. When antioxidant enzymes in blood and
liver tissue were examined, time-dependent decreases in activity were determined in
SOD, CAT, GPx, and GSH enzymes, while increased levels of MDA and MPO parameters
were observed in respect to Fe3O4-MNPs exposure. It was found that TNF-alpha, Il-6
levels were enhanced against Fe3O4-MNPs treatment, but Nrf-2 levels were decreased
at the 46th and 96th h. In the 96th application results, all parameters were
statistically significant (p < 0.05) in blood and liver tissue, except for the IL-6
results. It was determined that the frequency of MN, the level of 8-OHdG and
caspase-3 activity increased in respect to Fe3O4-MNPs exposure over time. Treatment
with UX alleviated Fe3O4-MNPs-induced hematotoxic and hepatotoxic alterations as
well as oxidative and genetic damages. Our findings offer strong evidence for the
use of UX as promising, safe and natural protective agents against environmental
toxicity of magnetite nanoparticles.
AN - rayyan-553781508
AU - Ucar, A.
AU - Arslan, M. E.
AU - Yeltekin, A. C.
AU - Ozgeris, F. B.
AU - Yildirim, O. C.
AU - Parlak, V.
AU - Alak, G.
AU - Turkez, H.
AU - Atamanalp, M.
DO - 10.1080/01480545.2022.2164298
KW - Oxalic Acid
Iron
Norisoprenoids
PY - 2023
SN - 0148-0545 1525-6014
ST - Neutralization of iron oxide magnetic nanoparticle aquatoxicity on
Oncorhynchus mykiss via supplementation with ulexite
T2 - DRUG AND CHEMICAL TOXICOLOGY
TI - Neutralization of iron oxide magnetic nanoparticle aquatoxicity on
Oncorhynchus mykiss via supplementation with ulexite
Y2 - 1 y3 - 5
ID - 9447
ER -
TY - JOUR
AB - The prevalent exposition of metallic nanoparticles (MNPs) to the aquatic
medium and their negative influence on human life is one of the major concerns
global. Stress mechanization, as a non-specific and pervasive response, involves
all physiological systems, particularly the closely interconnected neuroendocrine
and immune systems. In this study, which was designed to obtain more data on the
biological effects of ulexit, which prevents oxidative DNA damage by protecting
against toxicity damage and offers new antioxidant roles. The concomitant use of
ulexite (UX, as 18.75 mg/l) as a natural therapeutic agent against exposure to
magnetic nanoparticles (Fe3O4-MNPs/0.013 ml/l) on Oncorhynchus mykiss was
investigated for 96 h. The brain tissues were taken at the 48th and 96th hours of
the trial period, the effects on neurotoxic, pro-inflammatory cytokine genes,
antioxidant immune system, DNA and apoptosis mechanisms were analyzed. In the
present study, it was determined that AChE activity and BDNF level in the brain
tissue decreased over time in the Fe3O4-MNPs group compared to the control, and UX
tried to depress this inhibition. While inhibition was determined in antioxidant
system biomarkers (SOD, CAT, GPx, and GSH values), an induction was observed in
lipid peroxidation indicators (MDA and MPO values) in Fe3O4-MNPs applied group. The
same group data showed that TNF-alpha, IL-6, 8-OHdG and caspase-3 levels were
increased, but Nrf-2 levels were decreased. The alterations in all biomarkers were
found to be significant at the p < 0.05 level. In general, it was determined that
Fe3O4-MNPs caused stress in O. mykiss and UX exhibited a positive effect on this
stress management.
AN - rayyan-553781509
AU - Ucar, A.
AU - Parlak, V.
AU - Ozgeris, F. B.
AU - Yeltekin, A. C.
AU - Arslan, M. E.
AU - Alak, G.
AU - Turkez, H.
AU - Kocaman, E. M.
AU - Atamanalp, M.
DO - 10.1016/j.scitotenv.2022.155718
KW - Brain
Oxidative Stress
Antioxidants
PY - 2022
SN - 0048-9697 1879-1026
ST - Magnetic nanoparticles-induced neurotoxicity and oxidative stress in brain of
rainbow trout: Mitigation by ulexite through modulation of antioxidant, anti-
inflammatory, and antiapoptotic activities
T2 - SCIENCE OF THE TOTAL ENVIRONMENT
TI - Magnetic nanoparticles-induced neurotoxicity and oxidative stress in brain of
rainbow trout: Mitigation by ulexite through modulation of antioxidant, anti-
inflammatory, and antiapoptotic activities
VL - 838
Y2 - 9 y3 - 10
ID - 9448
ER -
TY - JOUR
AB - The potential for ingestion of copper oxide nanomaterials (CuO NMs) is
increasing due to their increased exploitation. Investigation of changes in gene
expression allows toxicity to be detected at an early stage of NM exposure and can
enable investigation of the mechanism of toxicity. Here, undifferentiated Caco-2
cells, differentiated Caco-2 cells, Caco-2/HT29-MTX (mucus secreting) and
Caco-2/Raji B (M cell model) co-cultures were exposed to CuO NMs and copper
sulphate (CuSO4) in order to determine their impacts. Cellular responses were
measured in terms of production of reactive oxygen species (ROS), the gene
expression of an antioxidant (haem oxygenase 1 (HMOX1)), the pro-inflammatory
cytokine (interleukin 8 (IL8)), the metal binding (metallothionein 1A and 2A (MT1A
and MT2A)) and the mucus secreting (mucin 2 (MUC2)), as well as HMOX-1 protein
level. While CuSO4 induced ROS production in cells, no such effect was observed for
CuO NMs. However, these particles did induce an increase in the level of HMOX-1
protein and upregulation of HMOX1, MT2A, IL8 and MUC2 genes in all cell models. In
conclusion, the expression of HMOX1, IL8 and MT2A were responsive to CuO NMs at 4
to 12 h post exposure when investigating the toxicity of NMs using intestinal in
vitro models. These findings can inform the selection of endpoints, timepoints and
models when investigating NM toxicity to the intestine in vitro in the future.
AN - rayyan-553781510
AU - Ude, V. C.
AU - Brown, D. M.
AU - Stone, V.
AU - Leonard, M.
DO - 10.1016/j.tiv.2021.105161
PY - 2021
SN - 0887-2333 1879-3177
ST - Time dependent impact of copper oxide nanomaterials on the expression of
genes associated with oxidative stress, metal binding, inflammation and mucus
secretion in single and co-culture intestinal in vitro models
TI - Time dependent impact of copper oxide nanomaterials on the expression of
genes associated with oxidative stress, metal binding, inflammation and mucus
secretion in single and co-culture intestinal in vitro models
VL - 74
Y2 - 8
ID - 9449
ER -
TY - JOUR
AB - Zinc oxide (ZnO) NPs, owing to their broad biomedical applications, have
recently attracted the scientific community with incredible interest as therapeutic
agents. So, the present study aims at preparation of ZnO NPs, using Tragia
involucrata leaf extract and exploring their capability as antioxidant, anticancer
and anti-inflammatory agents. Besides, the ointment formulation and electrochemical
studies were also carried out in this work. The antioxidant activity of the
synthesized ZnO NPs was evaluated using DPPH assay method and the results clearly
showed higher inhibition of about 70% and lower inhibition of about 14% for 100 mu
g/ml and 25 mu g/ml concentrations, respectively. The cytotoxic effects of ZnO NPs
were evaluated against human cancer cell lines such as A549 (lungs), HeLa
(cervical), HeP-2 (laryngeal) and MCF-7 (breast). The outcome of this investigation
confirmed the effectiveness of the synthesized NPs against HeP-2 even at the lowest
concentration. The anti-inflammatory activity was measured by the inhibition of
protein denaturation assay. A higher inhibition of about 54% was noticed at the
concentration of 100 mu g/ml. In the case of the ointment formulation study, the
pastes prepared using the biosynthesized ZnO NPs and commercially available ZnO
powder were compared and evaluated using the parameters such as pH, spreadability,
moisture content, extrudability, foamability and physical examinations. As it has
been noticed that all the observed parameters were matching well with those of the
commercially available ZnO powder, ZnO NPs, synthesized using Tragia involucrata,
may be suggested for the clinical trials. Cyclic voltammetry was used to measure
the specific capacitance of the synthesized ZnO NPs for different scan rates. The
results of this study showed the gradual decrease in specific capacitance value for
the corresponding increase in scan rates. Therefore, the results of present study
indicated that ZnO NPs prepared using Tragia involucrata leaves were found to be
effective for all the above chosen applications and hence, have multifunctional
capacity.
AN - rayyan-553781511
AU - Udhayan, S.
AU - Udayakumar, R.
AU - Gurusamy, K.
AU - Kalaichelvan, V. K.
AU - Gopalasatheeskumar, K.
DO - 10.1007/s12010-022-03866-z
IS - 6
KW - Ointments
PY - 2023
SN - 0273-2289 1559-0291
SP - 3764-3786
ST - Tragia involucrata Leaf-Mediated ZnO NPs: Biomedical Applications, Ointment
Formulation and Electrochemical Studies
T2 - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
TI - Tragia involucrata Leaf-Mediated ZnO NPs: Biomedical Applications, Ointment
Formulation and Electrochemical Studies
VL - 195
Y2 - 6
ID - 9450
ER -
TY - JOUR
AB - Diabetes wounds take longer to heal due to extended inflammation, decreased
angiogenesis, bacterial infection, and oxidative stress. These factors underscore
the need for biocompatible and multifunctional dressings with appropriate
physicochemical and swelling properties to accelerate wound healing. Herein,
insulin (Ins)-loaded, and silver (Ag) coated mesoporous polydopamine (mPD)
nanoparticles were synthesized (Ag@Ins-mPD). The nanoparticles were dispersed into
polycaprolactone/methacrylated hyaluronate aldehyde dispersion, electrospun to form
nanofibers, and then photochemically crosslinked to form a fibrous hydrogel. The
nanoparticle, fibrous hydrogel, and nanoparticle-reinforced fibrous hydrogel were
characterized for their morphological, mechanical, physicochemical, swelling, drug-
release, antibacterial, antioxidant, and cytocompatibility properties. The diabetic
wound reconstruction potential of nanoparticle-reinforced fibrous hydrogel was
studied using BALB/c mice. The results indicated that Ins-mPD acted as a reductant
to synthesize Ag nanoparticles on their surface, held antibacterial and antioxidant
potential, and their mesoporous properties are crucial for insulin loading and
sustained release. The nanoparticle-reinforced scaffolds were uniform in
architecture, porous, mechanically stable, showed good swelling, and possessed
superior antibacterial, and cell-responsive properties. Furthermore, the designed
fibrous hydrogel scaffold demonstrated good angiogenic, anti-inflammatory,
increased collagen deposition, and faster wound repair capabilities, therefore, it
could be used as a potential candidate for diabetic wound treatment. © 2023
Elsevier B.V.
AN - rayyan-553781512
AU - Ullah, S.
AU - Hussain, Z.
AU - Ullah, I.
AU - Wang, L.
AU - Mehmood, S.
AU - Liu, Y.
AU - Mansoorianfar, M.
AU - Liu, X.
AU - Ma, F.
AU - Pei, R.
DO - 10.1016/j.ijbiomac.2023.125738
KW - Diabetic wound healing
Fibrous hydrogel
Hyaluronic acid
Insulin
Mesoporous nanoparticles
Polydopamine
Antioxidants
Biocompatibility
Glucose
Hydrogels
Mammals
Mesoporous materials
Reinforcement
collagen
hyaluronic acid
hydrogel
insulin
mesoporous polydopamine nanoparticle
nanoparticle
polycaprolactone
silver nanoparticle
unclassified drug
Antibacterials
Diabetic wounds
Hyaluronate
Mesoporous
Mesoporous nanoparticle
Property
Wound healing
animal experiment
animal model
animal tissue
Article
cross linking
diabetic wound
dispersion
drug synthesis
electrospinning
freeze drying
male
molecular weight
mouse
nonhuman
physical chemistry
slow drug release
wound healing
Wound Healing
Hydrogel
PY - 2023
ST - Mussel bioinspired, silver-coated and insulin-loaded mesoporous polydopamine
nanoparticles reinforced hyaluronate-based fibrous hydrogel for potential diabetic
wound healing
TI - Mussel bioinspired, silver-coated and insulin-loaded mesoporous polydopamine
nanoparticles reinforced hyaluronate-based fibrous hydrogel for potential diabetic
wound healing
85165947835&doi=10.1016%2fj.ijbiomac.2023.125738&partnerID=40&md5=36124586917f58b1b
cf7b26e174225a9
VL - 247
ID - 9451
ER -
TY - JOUR
AB - Inflammatory cells orchestrate tumor niche for the proliferating neoplastic
cells, leading to neoangiogenesis, lymphangiogenesis, tumor growth and metastasis.
Emergence of severe side effects, multiple drug resistance and associated high cost
has rendered conventional chemotherapy less effectual. The aim was to develop a
multipurpose, less toxic, more potent and cheaper, oral non-conventional anticancer
therapeutic. Cyclooxygenase associated with tumor niche inflammation and
proliferative neoplastic cells were targeted synergistically, through anti-
inflammatory and anti-proliferative effects of model drug, diclofenac sodium and
fluorescent silver nanoparticles (AgNPs), respectively. Drug entrapped AgNPs were
surface modified with PVA (for controlling particle size, preferred cellular
uptake, evading opsonization and improved dispersion). XRD, FTIR, DSC, TGA, LIBS,
particle size and surface plasmon resonance analysis confirmed the efficient drug
encapsulation and PVA coating with 62% loading efficiency. In-vitro, the
formulation exhibited 1st order release kinetics with sustained and maximal release
at slightly acidic conditions (pH 4.5) enabling the potential for passive tumor
targeting. Also, nanoparticles showed efficient protein denaturation inhibition
potential, hemo-compatibility (<0.8%) and potent anti-cancer activity (P < 0.05)
against breast cancer cell line (MCF-7). In-vivo, developed nanoparticles improved
pharmacokinetics (2.8 fold increased AUC, 6.9 h t(1/2), C(max) = 1.6 ± 0.03 μg/ml,
K(el) = 0.1) and pharmacodynamics manifested by potent anti-inflammatory, analgesic
and anti-pyretic effects (P < 0.05) at 20 fold lower doses. LD(50) determination
revealed a wide therapeutic window. The study showed promise of synthesized
nanomaterials as cheaper, less toxic, hemo-compatible, oral and more potent anti-
inflammatory and non-conventional fluorescent anti-cancer agents, vanquishing tumor
niche inflammation and repressing proliferation of malignant cells.
AN - rayyan-553782093
AU - Ur Rehman, F.
AU - Mazhar, K.
AU - Malik, A.
AU - Naz, S. S.
AU - Shah, K. U.
AU - Khan, A.
AU - Khan, S.
AU - Ahmed, R.
AU - Qaisar, S.
DO - 10.1016/j.msec.2021.111940
J2 - Mater Sci Eng C Mater Biol Appl
KW - *Antineoplastic Agents/pharmacology/therapeutic use
Humans
MCF-7 Cells
*Nanoparticles
*Neoplasms
Particle Size
Silver
LA - eng
N1 - Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan;
Nanosciences and Technology Department, National Centre for Physics, Islamabad,
Pakistan.; Institute of Biomedical and Genetic Engineering (IBGE), Islamabad,
Pakistan.; Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan;
Nanosciences and Technology Department, National Centre for Physics, Islamabad,
Pakistan.; Nanosciences and Technology Department, National Centre for Physics,
Islamabad, Pakistan. Electronic address: organicsoul84@gmail.com.; Department of
Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan. Electronic address:
kushah@qau.edu.pk.; Department of Pharmacy, Quaid-i-Azam University, Islamabad,
Pakistan.; Department of Pharmacy, Quaid-i-Azam University, Islamabad, Pakistan.;
National Centre for Physics, Quaid-i-Azam University Campus, Islamabad, 45320,
Pakistan.; Nanosciences and Technology Department, National Centre for Physics,
Islamabad, Pakistan.
PY - 2021
SP - 111940
ST - Surface modified multifaceted nanocarriers for oral non-conventional cancer
therapy; synthesis and evaluation
T2 - Materials science & engineering. C, Materials for biological applications
TI - Surface modified multifaceted nanocarriers for oral non-conventional cancer
therapy; synthesis and evaluation
VL - 123
Y2 - 4
ID - 10004
ER -
TY - JOUR
AB - Neoplastic cells have co-opted inflammatory receptors and signaling molecules
that potentiate inflammation. Activated inflammatory pathways lead to neo-
angiogenesis, lymph-angiogenesis, immunosuppression, tumor growth, proliferation
and metastasis. This cancer-sustaining inflammation is a critical target to arrest
cancer growth. Multiple drug resistance, high cost, low oral bioavailability and
serious side effects have rendered conventional cytotoxic chemotherapeutics less
impressive. The aim of this research was to achieve cancer debulking and
proliferation prevention by limiting ‘cancer-sustaining’ tumor niche inflammation
through non-conventional oral approach employing anti-inflammatory agents and
avoiding conventional cytotoxic agents. Synergistic anti-inflammatory agents, i.e.
celecoxib as selective COX-2 inhibitor and montelukast as cysteinyl leukotriene
receptor antagonist, were selected. Silver nanoparticles (AgNPs) were used as
nanocarriers because of their efficient synergistic anti-neoplastic effects and
excellent oral drug delivery potential. Specifically, selected drugs were co-
conjugated onto AgNPs. Synthesized nanoparticles were then surface-modified with
poly (vinyl alcohol) to control particle size, avoid opsonization/preferred
cellular uptake and improve dispersion. Surface plasmon resonance analysis,
particle size analysis, DSC, TGA, XRD, FTIR and LIBS analysis confirmed the
successful conjugation of drugs and efficient polymer coating with high loading
efficiency. In-vitro, the nanoparticles manifested best and sustained release in
moderately acidic (pH 4.5) milieu enabling passive tumor targeting potential. In-
vivo, synthesized nanoparticles exhibited efficient dose-dependent anti-
inflammatory activity reducing the dose up to 25-fold. The formulation also
manifested hemo-compatibility, potent anti-denaturation activity and dose-dependent
in-vitro and in-vivo anti-cancer potential against MCF-7 breast cancer and Hep-G2
liver cancer cell lines in both orthotopic and subcutaneous xenograft cancer
models. The anti-inflammatory nanoparticles manifested tumor specific release
potential exhibiting selective cytotoxicity at cancerous milieu with slightly
acidic environment and activated inflammatory pathways. The formulation displayed
impressive oral bioavailability, sustained release, negligible cytotoxicity against
THLE-2 normal human hepatocytes, low toxicity (high LD50) and wide therapeutic
window. Results suggest promise of developed nanomaterials as hemo-compatible,
potent, cheaper, less-toxic oral anti-inflammatory and non-conventional anti-cancer
agents. © 2022, Iran University of Science and Technology. All rights reserved.
AN - rayyan-553781513
AU - Ur Rehman, F.
AU - Naz, S. S.
AU - Dar, M. J.
AU - Malik, A.
AU - Qindeel, M.
AU - Baino, F.
AU - Wahid, F.
AU - Rahdar, A.
AU - Munir, S.
AU - Qaisar, S.
AU - Shah, K. U.
AU - Razlansari, M.
IS - 2
KW - Anti-cancer
Anti-inflammatory
Drug delivery
Nanoparticles
Non-conventional chemotherapy
Mouth Neoplasms
PY - 2022
ST - Multifunctional Silver-based Nanomaterials for Non-conventional Oral Cancer
Therapy through Simultaneous LOX and Selective COX-2 inhibition
T2 - Iranian Journal of Materials Science and Engineering
TI - Multifunctional Silver-based Nanomaterials for Non-conventional Oral Cancer
Therapy through Simultaneous LOX and Selective COX-2 inhibition
85142651332&partnerID=40&md5=e3ffaf2282d0af703083c9ba0e6ce758
VL - 19
ID - 9452
ER -
TY - JOUR
AB - OBJECTIVE: Colorectal cancer remains the fourth most common cause of cancer-
related mortality worldwide. Here we investigate the role of nuclear factor-κB (NF-
κB) co-factor B-cell CLL/lymphoma 3 (BCL-3) in promoting colorectal tumour cell
survival. DESIGN: Immunohistochemistry was carried out on 47 tumour samples and
normal tissue from resection margins. The role of BCL-3/NF-κB complexes on cell
growth was studied in vivo and in vitro using an siRNA approach and exogenous BCL-3
expression in colorectal adenoma and carcinoma cells. The question whether BCL-3
activated the AKT/protein kinase B (PKB) pathway in colorectal tumour cells was
addressed by western blotting and confocal microscopy, and the ability of 5-
aminosalicylic acid (5-ASA) to suppress BCL-3 expression was also investigated.
RESULTS: We report increased BCL-3 expression in human colorectal cancers and
demonstrate that BCL-3 expression promotes tumour cell survival in vitro and tumour
growth in mouse xenografts in vivo, dependent on interaction with NF-κB p50 or p52
homodimers. We show that BCL-3 promotes cell survival under conditions relevant to
the tumour microenvironment, protecting both colorectal adenoma and carcinoma cells
from apoptosis via activation of the AKT survival pathway: AKT activation is
mediated via both PI3K and mammalian target of rapamycin (mTOR) pathways, leading
to phosphorylation of downstream targets GSK-3β and FoxO1/3a. Treatment with 5-ASA
suppressed BCL-3 expression in colorectal cancer cells. CONCLUSIONS: Our study
helps to unravel the mechanism by which BCL-3 is linked to poor prognosis in
colorectal cancer; we suggest that targeting BCL-3 activity represents an exciting
therapeutic opportunity potentially increasing the sensitivity of tumour cells to
conventional therapy.
AN - rayyan-553782030
AU - Urban, B. C.
AU - Collard, T. J.
AU - Eagle, C. J.
AU - Southern, S. L.
AU - Greenhough, A.
AU - Hamdollah-Zadeh, M.
AU - Ghosh, A.
AU - Poulsom, R.
AU - Paraskeva, C.
AU - Silver, A.
AU - Williams, A. C.
DO - 10.1136/gutjnl-2014-308270
IS - 7
J2 - Gut
KW - Animals
Anti-Inflammatory Agents, Non-Steroidal/pharmacology
Apoptosis
B-Cell Lymphoma 3 Protein
Cell Proliferation
Colon/chemistry
Colorectal Neoplasms/*chemistry/pathology
HCT116 Cells
Humans
Mesalamine/pharmacology
Mice
Mice, Nude
NF-kappa B/analysis/*metabolism
PTEN Phosphohydrolase/metabolism
Phosphatidylinositol 3-Kinases/metabolism
Proto-Oncogene Proteins/*analysis/genetics/*metabolism
Proto-Oncogene Proteins c-akt/*metabolism
RNA, Small Interfering/pharmacology
Rectum/chemistry
*Signal Transduction
TOR Serine-Threonine Kinases/metabolism
Transcription Factors/*analysis/genetics/*metabolism
Tumor Burden
LA - eng
N1 - School of Cellular & Molecular Medicine, University of Bristol, Bristol, UK.;
School of Cellular & Molecular Medicine, University of Bristol, Bristol, UK.;
Centre for Digestive Diseases, National Centre for Bowel Research and Surgical
Intervention, Blizard Institute of Cell and Molecular Science, Barts and The London
School of Medicine and Dentistry, Queen Mary University of London, Whitechapel,
London, UK.; Centre for Digestive Diseases, National Centre for Bowel Research and
Surgical Intervention, Blizard Institute of Cell and Molecular Science, Barts and
The London School of Medicine and Dentistry, Queen Mary University of London,
Whitechapel, London, UK.; School of Cellular & Molecular Medicine, University of
Bristol, Bristol, UK.; Centre for Digestive Diseases, National Centre for Bowel
Research and Surgical Intervention, Blizard Institute of Cell and Molecular
Science, Barts and The London School of Medicine and Dentistry, Queen Mary
University of London, Whitechapel, London, UK.; School of Cellular & Molecular
Medicine, University of Bristol, Bristol, UK.
PY - 2016
SP - 1151-64
ST - BCL-3 expression promotes colorectal tumorigenesis through activation of AKT
signalling
T2 - Gut
TI - BCL-3 expression promotes colorectal tumorigenesis through activation of AKT
signalling
VL - 65
Y2 - 7
ID - 9945
ER -
TY - JOUR
AB - Rambutan (Nephelium lappaceum) is a traditional fruit originally from Asia
and now widely available in Central America. The rind of rambutan is rich in
antioxidants, especially polyphenols, which have natural anti-inflammatory and
antimicrobial properties. Rambutan polyphenolic extract (RPE) was encapsulated in
multi-component electrospun nanofiber (ESNF) templates. These nanofibers were
decorated with silver nanoparticles (AgNPs) via in situ electrochemical reduction.
The RPE-loaded and AgNPs-decorated ESNF (AgNPs/RPE-ESNF) were evaluated for their
hydrophilic, antioxidant, and antimicrobial properties. Results indicate that the
addition of RPE to synthetic polycaprolactone (PCL) ESNF improves hydrophilicity
and cell attachment and proliferation in vitro. In addition, a synergistic
antibacterial and cell proliferation effect between AgNPs and RPE was observed when
they were combined in the multi-functionalized ESNF system. The effectiveness of
AgNPs/RPE-PCL ESNF as an antioxidant and antibacterial matrix could potentially
promote wound healing and also serve as a hybrid scaffold for tissue engineering.
AN - rayyan-553781514
AU - Urena-Saborio, H.
AU - Rodriguez, G.
AU - Madrigal-Carballo, S.
AU - Gunasekaran, S.
DO - 10.1016/j.mtla.2020.100687
KW - Polyphenols
PY - 2020
SN - 2589-1529
ST - Characterization and applications of silver nanoparticles-decorated
electrospun nanofibers loaded with polyphenolic extract from rambutan (Nepelium
lappaceum)
T2 - MATERIALIA
TI - Characterization and applications of silver nanoparticles-decorated
electrospun nanofibers loaded with polyphenolic extract from rambutan (Nepelium
lappaceum)
VL - 11
Y2 - 6
ID - 9453
ER -
TY - JOUR
AB - The respiratory tract is one of the most accessible ones to exogenous
nanoparticles, yet drug delivery by their means to it is made extraordinarily
challenging because of the plexus of aerodynamic, hemodynamic and biomolecular
factors at cellular and extracellular levels that synergistically define the safety
and efficacy of this process. Here, the use of inorganic nanoparticles (INPs) for
inhalable diagnostics and therapies of the lung is viewed through the prism of the
history of studies on the interaction of INPs with the lower respiratory tract. The
most conceptually and methodologically innovative and illuminative studies are
referred to in the chronological order, as they were reported in the literature,
and the trends in the progress of understanding this interaction of immense
therapeutic and toxicological significance are being deduced from it. The most
outstanding actual trends delineated include the diminishment of toxicity via
surface functionalization, cell targeting, tagging and tracking via controlled
binding and uptake, hybrid INP treatments, magnetic guidance, combined drug and
gene delivery, use as adjuvants in inhalable vaccines, and other. Many of the
understudied research directions, which have been accomplished by the
nanostructured organic polymers in the pulmonary niche, are discussed. The progress
in the use of INPs as inhalable diagnostics or therapeutics has been hampered by
their well-recognized inflammatory potential and toxicity in the respiratory tract.
However, the annual numbers of methodologically innovative studies have been on the
rise throughout the past two decades, suggesting that this is a prolific direction
of research, its comparatively poor commercial takings notwithstanding. Still, the
lack of consensus on the effects of many INP compositions at low but
therapeutically effective doses, the plethora of contradictory reports on
ostensibly identical chemical compositions and NP properties, and the many cases of
antagonism in combinatorial NP treatments imply that the rational design of
inhalable medical devices based on INPs must rely on qualitative principles for the
most part and embrace a partially stochastic approach as well. At the same time,
the fact that the most studied INPs for pulmonary applications have been those with
some of the thickest records of pulmonary toxicity, e.g., carbon, silver, gold,
silica and iron oxide, is a silent call for the expansion of the search for new
inorganic compositions for use in inhalable therapies to new territories. © 2023
The Author(s)
AN - rayyan-553781515
AU - Uskoković, V.
DO - 10.1016/j.cis.2023.102903
KW - Aerosol
Inhaler
Lung
Nanoparticle
Pulmonary
Respiratory
Nanoparticles
Nanostructures
Pharmaceutical Preparations
Polymers
Biological organs
Diagnosis
Gene transfer
Iron oxides
Organic polymers
Silica
Stochastic systems
drug
nanomaterial
nanoparticle
polymer
Bio-molecular
Cellular levels
Haemodynamics
Inhalable
Respiratory tract
chemistry
Toxicity
PY - 2023
ST - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
T2 - Advances in Colloid and Interface Science
TI - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
85152625854&doi=10.1016%2fj.cis.2023.102903&partnerID=40&md5=dfdb3db920aec633661e39
b131e190cb
VL - 315
ID - 9454
ER -
TY - JOUR
AB - The respiratory tract is one of the most accessible ones to exogenous
nanoparticles, yet drug delivery by their means to it is made extraordinarily
challenging because of the plexus of aerodynamic, hemodynamic and biomolecular
factors at cellular and extracellular levels that synergistically define the safety
and efficacy of this process. Here, the use of inorganic nanoparticles (INPs) for
inhalable diagnostics and therapies of the lung is viewed through the prism of the
history of studies on the interaction of INPs with the lower respiratory tract. The
most conceptually and methodologically innovative and illuminative studies are
referred to in the chronological order, as they were reported in the literature,
and the trends in the progress of understanding this interaction of immense
therapeutic and toxicological significance are being deduced from it. The most
outstanding actual trends delineated include the diminishment of toxicity via
surface functionalization, cell targeting, tagging and tracking via controlled
binding and uptake, hybrid INP treatments, magnetic guidance, combined drug and
gene delivery, use as adjuvants in inhalable vaccines, and other. Many of the
understudied research directions, which have been accomplished by the
nanostructured organic polymers in the pulmonary niche, are discussed. The progress
in the use of INPs as inhalable diagnostics or therapeutics has been hampered by
their well-recognized inflammatory potential and toxicity in the respiratory tract.
However, the annual numbers of methodologically innovative studies have been on the
rise throughout the past two decades, suggesting that this is a prolific direction
of research, its comparatively poor commercial takings notwithstanding. Still, the
lack of consensus on the effects of many INP compositions at low but
therapeutically effective doses, the plethora of contradictory reports on
ostensibly identical chemical compositions and NP properties, and the many cases of
antagonism in combinatorial NP treatments imply that the rational design of
inhalable medical devices based on INPs must rely on qualitative principles for the
most part and embrace a partially stochastic approach as well. At the same time,
the fact that the most studied INPs for pulmonary applications have been those with
some of the thickest records of pulmonary toxicity, e.g., carbon, silver, gold,
silica and iron oxide, is a silent call for the expansion of the search for new
inorganic compositions for use in inhalable therapies to new territories.
AN - rayyan-553781516
AU - Uskokovic, V.
DO - 10.1016/j.cis.2023.102903
KW - Nebulizers and Vaporizers
PY - 2023
SN - 0001-8686 1873-3727
ST - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
T2 - ADVANCES IN COLLOID AND INTERFACE SCIENCE
TI - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
VL - 315
Y2 - 5
ID - 9455
ER -
TY - JOUR
AB - Metallic nanoparticles (MNPs) are becoming widespread environmental
contaminants. They are currently added to several food preparations and cause a
fast-growing concern for human health. The present work aims to assess the impact
of zinc oxide (ZnO), titanium dioxide (TiO(2)), and silver (Ag) nanoparticles (NPs)
on the human gut metabolome and microbiome. Water samples spiked with two different
concentrations of each MNPs were subjected to in-vitro gastrointestinal digestion
and in-vitro large intestine fermentation. The effects of the treatments were
determined through 16 S amplicon sequencing and untargeted metabolomics. Multi-
omics data integration was then applied to correlate the two datasets. MNPs
treatments modulated the microbial genera Bifidobacterium, Sutterella, Escherichia
and Bacteroides. The treatments, especially the lower concentrations of Ag and ZnO,
caused modulation of indole derivatives, peptides, and metabolites related to
protein metabolism in the large intestine. Notably, these metabolites are
implicated in ulcerative colitis and inflammatory bowel disease. TiO(2) NPs
treatment in all concentrations increased E.coli relative abundance and decreased
the abundance of B. longum. Moreover, for TiO2(,) an enrichment in proinflammatory
lipid mediators of arachidonic acid metabolites, such as prostaglandin E2 and
leukotrienes B4, was detected. For all metals except TiO(2,) low NP concentrations
promoted differentiated profiles, thus suggesting that MNPs aggregation can limit
adverse effects on living cells.
AN - rayyan-553782380
AU - Vaccari, F.
AU - Zhang, L.
AU - Giuberti, G.
AU - Grasso, A.
AU - Bandini, F.
AU - García-Pérez, P.
AU - Copat, C.
AU - Lucini, L.
AU - Dall'Asta, M.
AU - Ferrante, M.
AU - Puglisi, E.
DO - 10.1016/j.jhazmat.2023.131331
J2 - J Hazard Mater
KW - Humans
*Zinc Oxide/toxicity
Metagenomics
Fermentation
*Metal Nanoparticles/toxicity
Titanium
Metabolomics
Escherichia coli
Digestion
LA - eng
N1 - Department for Sustainable Food Process, Università Cattolica del Sacro
Cuore, Piacenza, Italy.; Department for Sustainable Food Process, Università
Cattolica del Sacro Cuore, Piacenza, Italy.; Department for Sustainable Food
Process, Università Cattolica del Sacro Cuore, Piacenza, Italy. Electronic address:
gianluca.giuberti@unicatt.it.; Department of Medical, Surgical Sciences and
Advanced Tehnologies Università degli studi di Catania, Piazza dell'Università 2,
95131 Catania, Italy.; Department for Sustainable Food Process, Università
Cattolica del Sacro Cuore, Piacenza, Italy.; Department for Sustainable Food
Process, Università Cattolica del Sacro Cuore, Piacenza, Italy; Nutrition and
Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food
Science and Technology, Univesidade de Vigo, Ourense Campus, 32004 Ourense, Spain.;
Department of Medical, Surgical Sciences and Advanced Tehnologies Università degli
studi di Catania, Piazza dell'Università 2, 95131 Catania, Italy.; Department for
Sustainable Food Process, Università Cattolica del Sacro Cuore, Piacenza, Italy.;
Department of Animal Science, Food, and Nutrition, Università Cattolica del Sacro
Cuore, Piacenza, Italy.; Department of Medical, Surgical Sciences and Advanced
Tehnologies Università degli studi di Catania, Piazza dell'Università 2, 95131
Catania, Italy.; Department for Sustainable Food Process, Università Cattolica del
Sacro Cuore, Piacenza, Italy.
PY - 2023
SP - 131331
ST - The impact of metallic nanoparticles on gut fermentation processes: An
integrated metabolomics and metagenomics approach following an in vitro digestion
and fecal fermentation model
T2 - Journal of hazardous materials
TI - The impact of metallic nanoparticles on gut fermentation processes: An
integrated metabolomics and metagenomics approach following an in vitro digestion
and fecal fermentation model
VL - 453
Y2 - 7 y3 - 5
ID - 10287
ER -
TY - JOUR
AB - Background: Silver nanoparticles (AgNP) have gained much attention in recent
years due to their biomedical applications, especially as antimicrobial agents.
AgNP may be used in poultry production as an alternative to the use of antibiotic
growth promoter. However, little is known about the impact of oral administration
of AgNP on the gut microbiota and the immune system. The aim of the present study
was to investigate the effects of AgNP on growth, hematological and immunological
profile as well as intestinal microbial composition in broilers challenged with
Campylobacter jejuni (C.jejuni). Results: AgNP did not affect the intestinal
microbial profile of birds. The body weight gain and the relative weights of bursa
and spleen were reduced when supplemented with AgNP. There was no difference with
respect to packed cell volume. However, the plasma concentrations of IgG and IgM
were lower in birds receiving AgNP compared to the non-supplemented control group.
The expression of TNF-alpha and NF-kB at mRNA level was significantly higher in
birds receiving AgNP. Conclusions: The application of AgNP via the drinking water
in the concentration of 50 ppm reduced broiler growth, impaired immune functions
and had no antibacterial effect on different intestinal bacterial groups, which may
limit the applicability of AgNP against C. jejuni in broiler chickens.
AN - rayyan-553781517
AU - Vadalasetty, K. P.
AU - Lauridsen, C.
AU - Engberg, R. M.
AU - Vadalasetty, R.
AU - Kutwin, M.
AU - Chwalibog, A.
AU - Sawosz, E.
DO - 10.1186/s12917-017-1323-x
KW - Campylobacter Infections
Chickens
PY - 2018
SN - 1746-6148
ST - Influence of silver nanoparticles on growth and health of broiler chickens
after infection with Campylobacter jejuni
T2 - BMC VETERINARY RESEARCH
TI - Influence of silver nanoparticles on growth and health of broiler chickens
after infection with Campylobacter jejuni
VL - 14
Y2 - 1 y3 - 2
ID - 9456
ER -
TY - JOUR
AB - BACKGROUND: Tumor necrosis factor (TNF) has pleiotropic functions during both
the demyelinating autoimmune disease multiple sclerosis (MS) and its murine model
experimental autoimmune encephalomyelitis (EAE). How TNF regulates disability
during progressive disease remains unresolved. Using a progressive EAE model
characterized by sustained TNF and increasing morbidity, this study evaluates the
role of unregulated TNF in exacerbating central nervous system (CNS) pathology and
inflammation. METHODS: Progressive MS was mimicked by myelin oligodendrocyte
glycoprotein (MOG) peptide immunization of mice expressing a dominant negative IFN-
γ receptor alpha chain under the human glial fibrillary acidic protein promoter
(GFAPγR1∆). Diseased GFAPγR1∆ mice were treated with anti-TNF or control monoclonal
antibody during acute disease to monitor therapeutic effects on sustained
disability, demyelination, CNS inflammation, and blood brain barrier (BBB)
permeability. RESULTS: TNF was specifically sustained in infiltrating macrophages.
Anti-TNF treatment decreased established clinical disability and mortality rate
within 7 days. Control of disease progression was associated with a decline in
myelin loss and leukocyte infiltration, as well as macrophage activation. In
addition to mitigating CNS inflammation, TNF neutralization restored BBB integrity
and enhanced CNS anti-inflammatory responses. CONCLUSIONS: Sustained TNF production
by infiltrating macrophages associated with progressive EAE exacerbates disease
severity by promoting inflammation and disruption of BBB integrity, thereby
counteracting establishment of an anti-inflammatory environment required for
disease remission.
AN - rayyan-553782353
AU - Valentin-Torres, A.
AU - Savarin, C.
AU - Hinton, D. R.
AU - Phares, T. W.
AU - Bergmann, C. C.
AU - Stohlman, S. A.
DO - 10.1186/s12974-016-0513-y
J2 - J Neuroinflammation
KW - Animals
Antibodies/pharmacology
Antigens, CD/metabolism
Blood-Brain Barrier/physiopathology
Calcium-Binding Proteins/metabolism
Capillary Permeability/genetics
Central Nervous System/*pathology
Encephalomyelitis, Autoimmune, Experimental/chemically induced/*pathology
Gene Expression Regulation/genetics
Glial Fibrillary Acidic Protein/genetics/metabolism
Humans
Interferon-gamma/genetics/metabolism
Macrophages/*metabolism
Mice
Mice, Transgenic
Microfilament Proteins/metabolism
Myelin-Oligodendrocyte Glycoprotein/toxicity
Neuroglia/pathology
Neutrophil Infiltration/drug effects/genetics
Peptide Fragments/toxicity
Tumor Necrosis Factor-alpha/immunology/*metabolism
Macrophages
Central Nervous System
Encephalomyelitis
Autoimmunity
LA - eng
N1 - Department of Neurosciences NC-30, Lerner Research Institute, The Cleveland
Clinic, 9500 Euclid Ave., Cleveland, OH, 44195, USA. valenta@ccf.org.; Department
of Neurosciences NC-30, Lerner Research Institute, The Cleveland Clinic, 9500
Euclid Ave., Cleveland, OH, 44195, USA. savaric@ccf.org.; Department of Pathology,
Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033,
USA. dhinton@usc.edu.; Malaria Vaccine Branch, Walter Reed Army Institute of
Research, Silver Spring, MD, 20910, USA. timothy.w.phares.ctr@mail.mil.; Department
of Neurosciences NC-30, Lerner Research Institute, The Cleveland Clinic, 9500
Euclid Ave., Cleveland, OH, 44195, USA. bergmac@ccf.org.; Department of
Neurosciences NC-30, Lerner Research Institute, The Cleveland Clinic, 9500 Euclid
Ave., Cleveland, OH, 44195, USA. stohlms2@ccf.org.
PY - 2016
SP - 46
ST - Sustained TNF production by central nervous system infiltrating macrophages
promotes progressive autoimmune encephalomyelitis
T2 - Journal of neuroinflammation
TI - Sustained TNF production by central nervous system infiltrating macrophages
promotes progressive autoimmune encephalomyelitis
VL - 13
Y2 - 2 y3 - 22
ID - 10261
ER -
TY - JOUR
AB - Eosinophilic inflammation is frequently observed in response to nanoparticle
(NP) exposure in airway rodent models of allergies where the number of eosinophils
is increased in lungs. Despite this, it is surprising that the potential cytotoxic
effect of NP, as well as their direct role on eosinophils is poorly documented. The
present study investigated how different NP can alter the biology of the human
eosinophilic cell line AML14.3D10. It was found that among NP forms of CeO2, ZnO,
TiO2, and nanosilver of 20 nm (AgNP20) or 70 nm (AgNP70) diameters, only ZnO and
AgNP20 induced apoptosis. Caspases-7 and -9 were not activated by the tested NP
while caspase-3 was activated by AgNP20 only. However, both ZnO and AgNP20 induced
cytoskeletal breakdown as evidenced by the cleavage of lamin B1. Using an ELISArray
approach for the simultaneous detection of several analytes (cytokines/chemokines),
it was found that only ZnO and AgNP20 increased the production of different
analytes including the potent pro-inflammatory CXCL8 (IL-8) chemokine. From the
data here, we conclude that toxic effects of some NP could be observed in human
eosinophil-like cells and that this could be related, at least partially, by
induction of apoptosis and production of cytokines and chemokines involved in
inflammation. The results of this study also indicate that distinct NP do not
activate similarly human eosinophils, since ZnO and AgNP20 induce apoptosis and
cytokine production while others such as TiO2, CeO2, and AgNP70 do not. © 2016
AN - rayyan-553781520
AU - Vallières, F.
AU - Simard, J. C.
AU - Noël, C.
AU - Murphy-Marion, M.
AU - Lavastre, V.
AU - Girard, D.
DO - 10.1080/1547691X.2016.1203379
IS - 6
KW - Apoptosis
cytokines
cytoskeleton
eosinophils
nanoparticles
Animals
Caspase 3
Cell Line
Cytoskeleton
Eosinophils
Humans
Immunomodulation
Lamin Type B
Metal Nanoparticles
Mice
Proteolysis
Rats
Silver
Zinc
caspase 3
caspase 7
caspase 9
interleukin 8
ITAC protein
lamin B
lamin b1
protein
RANTES
silver nanoparticle
unclassified drug
zinc oxide
lamin B1
metal nanoparticle
silver
zinc
apoptosis
Article
cell viability
controlled study
cytokine production
enzyme activation
eosinophil
human
human cell
priority journal
protein degradation
zeta potential
animal
cell line
immunology
immunomodulation
metabolism
mouse
rat
Humanities
Humanism
Cytokines
PY - 2016
SP - 817-826
ST - Activation of human AML14.3D10 eosinophils by nanoparticles: Modulatory
activity on apoptosis and cytokine production
T2 - Journal of Immunotoxicology
TI - Activation of human AML14.3D10 eosinophils by nanoparticles: Modulatory
activity on apoptosis and cytokine production
84978134362&doi=10.1080%2f1547691X.2016.1203379&partnerID=40&md5=3cf9f0a496348ef6ea
1ea144e1d62106
VL - 13
ID - 9459
ER -
TY - JOUR
AB - Dendritic cells (DCs) can acquire, process, and present antigens to T-cells
to induce an immune response. For this reason, targeting cancer antigens to DCs in
order to cause an immune response against cancer is an emerging area of
nanomedicine that has the potential to redefine the way certain cancers are
treated. The use of plasmonically active silver-coated gold nanorods (henceforth
referred to as plasmonic nano vectors (PNVs)) as potential carriers for DC tumor
vaccines has not been presented before. Effective carriers must be able to be
phagocytized by DCs, present low toxicity, and induce the maturation of DCs-an
early indication of an immune response. When we treated DCs with the PNVs, we found
that the cell viability of DCs was unaffected, up to 200 mu g/ml. Additionally, the
PNVs associated with the DCs as they were phagocytized and they were found to
reside within intracellular compartments such as endosomes. More importantly, the
PNVs were able to induce expression of surface markers indicative of DC activation
and maturation, i. e. CD40, CD86, and MHC class II. These results provide the first
evidence that PNVs are promising carriers for DC-based vaccines and warrant further
investigating for clinical use.
AN - rayyan-553781524
AU - Vang, K. B.
AU - Safina, I.
AU - Darrigues, E.
AU - Nedosekin, D.
AU - Nima, Z. A.
AU - Majeed, W.
AU - Watanabe, F.
AU - Kannarpady, G.
AU - Kore, R. A.
AU - Casciano, D.
AU - Zharov, V. P.
AU - Griffin, R. J.
AU - Dings, R. P. M.
AU - Biris, A. S.
DO - 10.1038/s41598-017-04459-1
KW - Dendritic Cells
PY - 2017
SN - 2045-2322
ST - Modifying Dendritic Cell Activation with Plasmonic Nano Vectors
TI - Modifying Dendritic Cell Activation with Plasmonic Nano Vectors
VL - 7
Y2 - 7 y3 - 14
ID - 9462
ER -
TY - JOUR
AB - It is becoming increasingly clear that nanoparticles (NPs) possess many
potential applications in both clinical medicine and research. Potential
utilization of NPs in nanomedicine for the treatment of respiratory diseases where
eosinophils exert pathogenic roles is gaining increasing attention. Even though
several NPs were found to possess pro-inflammatory activities in in vivo models
based on an increased number of eosinophils in rodent airways, it is not clear how
NPs could directly activate eosinophils themselves and how they can alter their
biology. In this review, we discuss the most recent data in this new area of
research demonstrating that NPs could now be added as new eosinophils modulators.
Indeed, activation of eosinophils with NPs could lead to modulation of spontaneous
apoptosis, caspase activation, and cytoskeleton breakdown when apoptosis is
induced; cytokine production, de novo protein synthesis, cellular adhesion onto a
cell substratum, and cell signalling events such as activation of the
phosphoinositide 3-kinase/Akt pathway and actin re-localization are involved in NP-
induced adhesion. Therefore, future development of therapeutic strategies with NPs
aiming at targeting diseases where eosinophils are involved should now consider the
capacity of NPs to modulate human eosinophil biology.
AN - rayyan-553781525
AU - Vanharen, M.
AU - Girard, D.
DO - 10.1007/s10753-019-01064-4
IS - 1
KW - Humanities
Humanism
Humans
PY - 2020
SN - 0360-3997 1573-2576
SP - 8-16
ST - Activation of Human Eosinophils with Nanoparticles: a New Area of Research
T2 - INFLAMMATION
TI - Activation of Human Eosinophils with Nanoparticles: a New Area of Research
VL - 43
Y2 - 2
ID - 9463
ER -
TY - JOUR
AB - Wound infections constitute an increasing clinical problem worldwide. To
reverse this trend, several wound dressings with antimicrobial properties have been
developed. Considering the increasing presence of antibiotic-resistant
microorganisms, product developers have been focusing their efforts in introducing
antibiotic-free antibacterial wound dressings to the market, with silver being the
most commonly incorporated antimicrobial agent. In this scenario, gaining
information about the microbial and eukaryotic cells' response to these dressings
is needed for a proper selection of antimicrobial dressings for the different cases
of infected wounds. In particular, one insufficiently explored parameter is the
effect of the dressings on the immunomodulation of macrophages, the main immune
cell population participating in the repair process, because of their pivotal role
in the transition of the inflammation to the proliferation phase of wound healing.
In this work, three different clinically applied antimicrobial, silver impregnated
wound dressings were selected: Atrauman(R) Ag, Biatain(R) Alginate Ag and PolyMem
WIC Silver(R) Non-adhesive. Antimicrobial susceptibility tests (disk diffusion and
broth dilution), cell viability evaluation (CellTiter-Blue(R)) and experiments to
determine macrophage polarization (e.g., flow cytometry, ELISA and glucose uptake)
were performed after 24 h of incubation. Among all products tested, Biatain(R)
Alginate Ag induced the most evident bactericidal effect on Gram-positive and Gram-
negative bacteria, followed by PolyMem WIC Silver(R) Non-adhesive, but did not show
good cytocompatibility in vitro. On the other hand, Atrauman(R) Ag showed excellent
cytocompatibility on L929 fibroblasts, HaCaT keratinocytes and THP-1 derived
macrophages, but no significant antimicrobial activity was observed. Overall, it
was confirmed that macrophages initiate, in fact, an alteration of their metabolism
and phenotype in response to wound dressings of different composition in a short
period of contact (24 h). M0 resting state macrophages common response to all
silver-containing dressings used in this study was to increase the production of
the anti-inflammatory cytokine TGF-beta, which indicates an acquisition of M2-like
macrophages characteristics.
AN - rayyan-553781526
AU - Varela, P.
AU - Marlinghaus, L.
AU - Sartori, S.
AU - Viebahn, R.
AU - Salber, J.
AU - Ciardelli, G.
DO - 10.3389/fbioe.2020.00124
KW - Humanities
Humanism
Humans
Macrophages
Bandages
PY - 2020
SN - 2296-4185
ST - Response of Human Macrophages to Clinically Applied Wound Dressings Loaded
With Silver
TI - Response of Human Macrophages to Clinically Applied Wound Dressings Loaded
With Silver
VL - 8
Y2 - 2 y3 - 25
ID - 9464
ER -
TY - JOUR
AB - Oral mucositis (OM) is among the most common, painful, and debilitating
toxicities of cancer regimen-related treatment, resulting in the formation of
ulcers, which are susceptible to increased colonization of microorganisms. Novel
discoveries in OM have focused on understanding the host-microbial interactions,
because current pathways have shown that major virulence factors from
microorganisms have the potential to contribute to the development of OM and may
even prolong the existence of already established ulcerations, affecting tissue
healing. Additional comprehensive and disciplined clinical investigation is needed
to carefully characterize the relationship between the clinical trajectory of OM,
the local levels of inflammatory changes (both clinical and molecular), and the ebb
and flow of the oral microbiota. Answering such questions will increase our
knowledge of the mechanisms engaged by the oral immune system in response to
mucositis, facilitating their translation into novel therapeutic approaches. In
doing so, directed clinical strategies can be developed that specifically target
those times and tissues that are most susceptible to intervention.
AN - rayyan-553781527
AU - Vasconcelos, R. M.
AU - Sanfilippo, N.
AU - Paster, B. J.
AU - Kerr, A. R.
AU - Li, Y.
AU - Ramalho, L.
AU - Queiroz, E. L.
AU - Smith, B.
AU - Sonis, S. T.
AU - Corby, P. M.
DO - 10.1177/0022034516641890
IS - 7
KW - Stomatitis
PY - 2016
SN - 0022-0345 1544-0591
SP - 725-733
ST - Host-Microbiome Cross-talk in Oral Mucositis
T2 - JOURNAL OF DENTAL RESEARCH
TI - Host-Microbiome Cross-talk in Oral Mucositis
VL - 95
Y2 - 7
ID - 9465
ER -
TY - JOUR
AB - The review summarizes the recent data on the toxic effect that engineered
metal nanoparticles (NPs) and their oxides (Ag, Au, Cu, CuO, zero-valent iron,
TiO2, and ZnO) exert on marine organisms of various taxonomic groups. Toxicity is
considered with due regard of the size effects and physicochemical properties of
NPs. Available data indicate that metallic NPs may adversely affect algae,
bacteria, mollusks, worms, arthropods, echinoderms, chordates, etc. Toxic effects
vary from reproductive disorders to death. Only scarce data are available on the NP
effect on marine vertebrates (e.g., marine fish). The factors that affect the NP
toxicity in an aqueous environment include the physicochemical properties of NPs,
species specificities of the test organism, and parameters of the aqueous
environment (in particular, salinity, temperature and pH). Exact relationships
between the factors and NP toxicity are still poorly understood. In particular,
there is no consensus on how salinity affects the NP toxicity in an aqueous
environment. Putative mechanisms of NP toxicity include oxidative stress, impaired
cell membrane integrity, inflammatory processes with consequent cell dysfunction,
DNA damage, and genetic mutations. NPs easily persist within organisms and
consequently have a high potential to accumulate in marine food chains. Lack of
standardized techniques to detect metallic and metal oxide NPs and weak or absent
relevant legislation complicate toxicology studies in marine ecosystems. The above
circumstances make it necessary to further evaluate the safety of metallic NPs for
marine organisms and especially vertebrates, to study the distant effects
associated with NP transition and accumulation in marine food chains, and to unify
the respective research protocols. The exact role of environmental factors in
modulating NP toxicity and the mechanisms of NP toxicity are also important issues
to address in future studies.Y
AN - rayyan-553781528
AU - Vasyukova, I. A.
AU - Zakharova, O. V.
AU - Chaika, V. V.
AU - Pikula, K. S.
AU - Golokhvast, K. S.
AU - Gusev, A. A.
DO - 10.1134/S2635167621020178
IS - 2
KW - Military Personnel
PY - 2021
SN - 2635-1676 2635-1684
SP - 138-154
ST - Toxic Effect of Metal-Based Nanomaterials on Representatives of Marine
Ecosystems: A Review
T2 - NANOBIOTECHNOLOGY REPORTS
TI - Toxic Effect of Metal-Based Nanomaterials on Representatives of Marine
Ecosystems: A Review
VL - 16
Y2 - 3
ID - 9466
ER -
TY - JOUR
AB - ABSTRACT Tooth bleaching is a technique of choice to obtain a harmonious
smile, but bleaching agents may damage the dental pulp. Objective: This study
evaluated the inflammatory responses of human dental pulp after the use of two
bleaching techniques. Material and Methods: Pulp samples were collected from human
third molars extracted for orthodontic reasons and divided into three groups:
control - no tooth bleaching (CG) (n=7); at-home bleaching with 15% carbamide
peroxide (AH) (n = 10), and in-office bleaching with 38% hydrogen peroxide (IO)
(n=12). Pulps were removed and stained with hematoxylin-eosin for microscopic
analysis of inflammation intensity, collagen degradation, and pulp tissue
organization. Immunohistochemistry was used to detect mast cells (tryptase+), blood
vessels (CD31+), and macrophages (CD68+). Chi-square, Kruskal-Wallis, and Mann
Whitney tests were used for statistical analysis. The level of significance was set
at p<.05. Results: The inflammation intensity and the number of macrophages were
significantly greater in IO than in AH and CG (p<0.05). The results of CD31+ (blood
vessels per mm2) were similar in CG (61.39±20.03), AH (52.29±27.62), and IO
(57.43±8.69) groups (p>0.05). No mast cells were found in the pulp samples
analyzed. Conclusion: In-office bleaching with 38% hydrogen peroxide resulted in
more intense inflammation, higher macrophages migration, and greater pulp damage
then at-home bleaching with 15% carbamide peroxide, however, these bleaching
techniques did not induce migration of mast cells and increased the number of blood
vessels.
AN - rayyan-553781529
AU - Vaz, Maysa Magalhães
AU - Lopes, Lawrence Gonzaga
AU - Cardoso, Paula Carvalho
AU - Souza, João Batista de
AU - Batista, Aline Carvalho
AU - Costa, Nádia Lago
AU - Torres, Érica Miranda
AU - Estrela, Carlos
DO - 10.1590/1678-775720160137
IS - 5
KW - Dental pulp
Inflammation
Microscopy
Tooth bleaching
Humanities
Humanism
Humans
Dental Pulp
LA - en
PY - 2016
SN - 1678-7757
SP - 509-517
ST - Inflammatory response of human dental pulp to at-home and in-office tooth
bleaching
T2 - J. appl. oral sci
TI - Inflammatory response of human dental pulp to at-home and in-office tooth
bleaching
77572016000500509
VL - 24
Y2 - 10 y3 - 1
ID - 9467
ER -
TY - JOUR
AB - The present investigation highlights a unique, rapid, greener, economical and
biological approach in the fabrication of gold (Au), copper (Cu), iron (Fe) and
zinc (Zn) monometallic nanoparticles (MNPs) using Annona muricata aqueous leaf
extract (AMLE) as a suitable reducing, capping and stabilizing agent. The bio-
fabricated NPs were characterized using UV-Visible spectrophotometer and field
emission scanning electron microscope with energy-dispersive x-ray spectroscopy
(FESEM-EDX). Surface plasmon resonance (SPR) determined the formation of MNPs with
maximum absorbance at 540, 570, 280 and 360 nm for Au, Cu, Fe and Zn-NPs
respectively. Among all the bio-fabricated MNPs investigated CuNPs have showed
superior activity for their antibacterial (against Escherichia coli), anti-
inflammatory (egg albumin protein denaturation inhibition-51.46 +/- 1.53 mu g/mL),
antidiabetic (alpha-glucosidase activity inhibition-32.97 +/- 2.51 mu g/mL), anti-
oxidative (DPPH free radical inhibition-63.44 +/- 3.12 mu g/mL) and anti-cancer
(against K562 cancer cell line using MTT assay-53.34 +/- 2.29 mu g/mL) potentials.
Whereas ZnNPs showed less-activity in anti-inflammatory and anti-oxidative (> 100
mu g/mL) and no activity was observed in anti-diabetic and anti-cancer assays
compared to other investigated NPs. Both Cuand Fe-NPs showed catalytic ability in
the degradation of textile dyes in the presence of sodium borohydride (NaBH4). Cu
and Fe-NPs degraded 94% of rhodamine-B (rh-B) under 35 and 90 min, whereas 94% of
methyl orange (MO) under 25 and 70 min, respectively. Eco-toxicity studies carried
out using Artemia nauplii have suggested that ZnNPs are less toxic (LC50 was > 100
mu g/mL after 24 h) compared to other investigated NPs.
AN - rayyan-553781531
AU - Velidandi, A.
AU - Pabbathi, N. P. P.
AU - Dahariya, S.
AU - Baadhe, R. R.
DO - 10.1016/j.colcom.2020.100302
KW - Tocopherols
PY - 2020
SN - 2215-0382
ST - Catalytic and eco-toxicity investigations of bio-fabricated monometallic
nanoparticles along with their anti-bacterial, anti-inflammatory, anti-diabetic,
anti-oxidative and anti-cancer potentials
T2 - COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
TI - Catalytic and eco-toxicity investigations of bio-fabricated monometallic
nanoparticles along with their anti-bacterial, anti-inflammatory, anti-diabetic,
anti-oxidative and anti-cancer potentials
VL - 38
Y2 - 9
ID - 9469
ER -
TY - JOUR
AB - There is a need for developing eco-friendly, cost-effective materials in
medical, biological, and agricultural fields, now, the problem can be resolved
using environment-friendly green approached metal oxide nanoparticles were used.
Therefore, the CuO nanoparticles were biosynthesized using Panicum sumatrense
grains aqueous extract for the first time. The gas chromatography-mass spectrometry
analysis revealed the diethyl phthalate (99.18%) was the major active phytocompound
in the grains extract. The biosynthesized CuO nanoparticles were characterized
using various analytical techniques. The UV-visible spectrum revealed the surface
Plasmon resonance band of CuO nanoparticles at 305 nm. X-ray diffraction pattern
showed the high crystalline nature of nanoparticles and their mean crystallite size
was at 25 nm. Fourier transform infra-red spectrum exposed the Cu-O stretching
vibrational bands at 830 and 510 cm(-1). The high resolution transmission electron
micrographs illustrated the cluster of rectangular shaped nanoparticles and their
average particle size was in between 15 and 35 nm. The energy dispersive spectrum
and mapping analyses showed the major presence of Cu and O in nanoparticles. The
antibacterial activity and its SEM images revealed the bactericidal characteristics
of CuO nanoparticles against Salmonella typhi and Staphylococcus aureus bacteria at
150 mu g/mL concentration. The maximum zone of inhibition of nanoparticles against
S. typhi and S. aureus bacterial cells were 22 and 26 mm, respectively. The
antioxidant, anti-inflammatory, and anti-diabetic analyses indicated the effectual
biological activities at 98.25, 92.65, and 89.10% in 80 mu g/mL concentration of
CuO nanoparticles, respectively. The morphological and tissue changes in Culex
tritaeniorhynchus mosquito larvae and Tribolium castaneum insect revealed the
effective larvicidal and insecticidal activity of CuO nanoparticles. The maximum
mortality of larvae and insect by CuO nanoparticles were 100 ppm at 48 h and 100 mu
g/Kg at 48, 72 h, 75 mu g/Kg at 72 h, respectively. The anticancer activity
divulged the effectual inhibition (95.69%) of CuO nanoparticles against MCF-7
breast cancer cells in low IC50 value at 11.49 mu g/mL. The activity against L929
(fibroblast) normal cells showed non-toxicity of CuO nanoparticles to normal cells.
The plant growth analysis explained the efficient growth of Vigna mungo plant at 60
ppm concentration of CuO nanoparticles. As per the results, the synthesized CuO
nanoparticles can be applied as a biocompatible, cost-effective insecticides, drugs
and fertilizers.
AN - rayyan-553781532
AU - Velsankar, K.
AU - Parvathy, G.
AU - Mohandoss, S.
AU - Kumar, R. M.
AU - Sudhahar, S.
DO - 10.1007/s13204-022-02441-6
IS - 6
KW - Cereals
PY - 2022
SN - 2190-5509 2190-5517
SP - 1993-2021
ST - Green synthesis and characterization of CuO nanoparticles using Panicum
sumatrense grains extract for biological applications
T2 - APPLIED NANOSCIENCE
TI - Green synthesis and characterization of CuO nanoparticles using Panicum
sumatrense grains extract for biological applications
VL - 12
Y2 - 6
ID - 9470
ER -
TY - JOUR
AB - Iron oxide nanoparticles have been used in diverse fields due to their
significant applications. The synthesis of iron oxide nanoparticles using plant
material extract is a simple, inexpensive and eco-friendly approach. The present
study demonstrates the effective bio-synthesis of iron oxide nanoparticles using
grains extract of Echinochloa frumentacea. GC-MS analysis report revealed the
information about the phytochemicals in aqueous grains extract. The formation of
iron oxide nanoparticles was confirmed using UV-visible, XRD, FTIR, HRTEM, EDX, and
mapping analyses. The UV-visible spectrum of iron oxide nanoparticles showed the
SPR band at 289 nm. The XRD studies confirmed the formation of high crystalline
iron oxide nanoparticles. HRTEM analysis showed that the formed nanoparticles were
rectangular and triangular in shape and the size ranged between 20 and 40 nm. The
iron oxide nanoparticles revealed significant antibacterial activity against the
microbes such as Staphylococcus aureus and Salmonella typhi at 150 mu g/mL and
morphological changes were observed. The high concentration of nanoparticles
exhibited 24 and 27 mm zone of inhibition on S. typhi and S. aureus, respectively.
We observed the role of biological activities such as antioxidant, anti-
inflammatory, and anti-diabetic properties of nanoparticles, and they showed
excellent bioactive properties even at a low IC50 value. The antioxidant,
antiinflammatory and anti-diabetic analyses showed high activity at 95.10, 92.10
and 91.68% for 80 mu g/mL concentration, respectively. Moreover, the iron oxide
nanoparticles exhibited high cytotoxicity (94.36%) against A549 cancer cells and no
toxicity against HEK293 normal cells which showed the biocompatibility of
nanoparticles. The larvicidal and insecticidal activities of nanoparticles showed
the maximum mortality at 48 h in 100 ppm and 72 h in 75, 100 mu g/kg, respectively.
The morphological and structural changes in larvae and insects validated the
effective biological activities of iron oxide nanoparticles. The plant growth study
results recommended that the lower concentration of nanoparticles at 60 ppm was
helpful for the germination of seeds and growth of root and stem in the Vigna
unguiculata plant. Finally, the study results suggested that the synthesized iron
oxide nanoparticles can potentially be useful in pharmaceutical, agricultural,
targeted drug delivery and biomedical applications.
AN - rayyan-553781533
AU - Velsankar, K.
AU - Parvathy, G.
AU - Mohandoss, S.
AU - Ravi, G.
AU - Sudhahar, S.
DO - 10.1016/j.jddst.2022.103799
KW - Drug Synergism
Oxalic Acid
Iron
Cereals
PY - 2022
SN - 1773-2247 2588-8943
ST - Echinochloa frumentacea grains extract mediated synthesis and
characterization of iron oxide nanoparticles: A greener nano drug for potential
biomedical applications
TI - Echinochloa frumentacea grains extract mediated synthesis and
characterization of iron oxide nanoparticles: A greener nano drug for potential
biomedical applications
VL - 76
Y2 - 10
ID - 9471
ER -
TY - JOUR
AB - In this report, the silver oxide nanoparticles were green synthesized using
Panicum miliaceum grains extract and were proposed for the first time. GC–MS
analysis explicated 2-Acetylbenzoic acid was the active phytocompound with 97.07%
of presence in aqueous grains extract. The synthesized silver oxide nanoparticles
were analyzed by several analytical techniques such as UV–visible, XRD, FT-IR, HR-
TEM, TG, XPS, EDX and mapping analyses. The results of various analytical
techniques confirmed the silver oxide nanoparticles formation. The formed
nanoparticles were in 10–25 nm size. The effectual bioactive properties of
nanoparticles were revealed through antioxidant, anti-diabetic, anti-inflammatory,
larvicidal and insecticidal activities. The high mortality of larvae and insect was
observed at 48 h in 100 ppm and 72 h in 100 μg/Kg concentration, respectively. The
antibacterial activity explained the bactericidal property of nanoparticles on S.
aureus and S. typhi at 150 μg/mL concentration. The effective drug activity of
nanoparticles was observed from 98.10 % of toxicity against A549 lung cancer cells
at 100 μg/mL concentration. The growth of Vigna unguiculata was efficiently
increased by lower concentration (60 ppm) of nanoparticles. According to results,
the green synthesized nanoparticles can be applied in pharmaceutical and
agricultural sectors as biocompatible, non-toxic and cost-effective material. ©
2022 The Society of Powder Technology Japan
AN - rayyan-553781534
AU - Velsankar, K.
AU - Parvathy, G.
AU - Sankaranarayanan, K.
AU - Mohandoss, S.
AU - Sudhahar, S.
DO - 10.1016/j.apt.2022.103645
IS - 7
KW - Anticancer activity
Green synthesis
Larvicidal activity
Rectangular shape
Silver oxide nanoparticles
Biocompatibility
Drug delivery
Grain (agricultural product)
Grain size and shape
Oxides
Silver oxides
Anticancer activities
Biological applications
GC/MS analysis
Larvicidal activities
Panicum
Rectangular shapes
Synthesised
UV-visible
Cost effectiveness
Cereals
PY - 2022
ST - Green synthesis of silver oxide nanoparticles using Panicum miliaceum grains
extract for biological applications
T2 - Advanced Powder Technology
TI - Green synthesis of silver oxide nanoparticles using Panicum miliaceum grains
extract for biological applications
85131404706&doi=10.1016%2fj.apt.2022.103645&partnerID=40&md5=bea535d9c6424ba5ed262a
0693dcc3bd
VL - 33
ID - 9472
ER -
TY - JOUR
AB - Oxidative stress and chronic inflammation interplay with the pathogenesis of
cancer. Breast cancer in women is the burning issue of this century, despite
chemotherapy and magnetic therapy. The management of secondary complications
triggered by post-chemotherapy poses a great challenge. Thus, identifying target-
specific drugs with anticancer potential without secondary complications is a
challenging task for the scientific community. It is possible that green technology
has been employed in a greater way in order to fabricate nanoparticles by
amalgamating plants with medicinal potential with metal oxide nanoparticles that
impart high therapeutic properties with the least toxicity. Thus, the present study
describes the synthesis of Titanium dioxide nanoparticles (TiO2 NPs) using aqueous
Terenna asiatica fruit extract, with its antioxidant, anti-inflammatory and
anticancer properties. The characterisation of TiO2 NPs was carried out using a
powdered X-ray diffractometer (XRD), Fourier transform infrared (FTIR), scanning
electron microscopy (SEM), energy-dispersive X-ray diffraction (EDX), high-
resolution transmission electron microscopy (HR-TEM), dynamic light scattering
(DLS), and zeta-potential. TiO2 NPs showed their antioxidant property by scavenging
1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals in a dose-dependent manner with
an IC50 value of 80.21 & mu;g/& mu;L. To ascertain the observed antioxidant
potential of TiO2 NPs, red blood cells (RBC) were used as an in vitro model system.
Interestingly, TiO2 NPs significantly ameliorated all the stress parameters, such
as lipid peroxidation (LPO), protein carbonyl content (PCC), total thiol (TT),
superoxide dismutase (SOD), and catalase (CAT) in sodium nitrite (NaNO2)-induced
oxidative stress, in RBC. Furthermore, TiO2 NPs inhibited RBC membrane lysis and
the denaturation of both egg and bovine serum albumin, significantly in a dose-
dependent manner, suggesting its anti-inflammatory property. Interestingly, TiO2
NPs were found to kill the MCF-7 cells as a significant decrease in cell viability
of the MCF-7 cell lines was observed. The percentage of growth inhibition of the
MCF-7 cells was compared to that of untreated cells at various doses (12.5, 25, 50,
100, and 200 & mu;g/mL). The IC50 value of TiO2 NPs was found to be (120 &
mu;g/mL). Furthermore, the Annexin V/PI staining test was carried out to confirm
apoptosis. The assay indicated apoptosis in cancer cells after 24 h of exposure to
TiO2 NPs (120 & mu;g/mL). The untreated cells showed no significant apoptosis in
comparison with the standard drug doxorubicin. In conclusion, TiO2 NPs potentially
ameliorate NaNO2-induced oxidative stress in RBC, inflammation and MCF-7 cells
proliferation.
AN - rayyan-553781536
AU - Venkatappa, M. M.
AU - Udagani, C.
AU - Gowda, S. H.
AU - Venkataramaiah, S.
AU - Casini, R.
AU - Moussa, I. M.
AU - Achur, R.
AU - Sannaningaiah, D.
AU - Elansary, H. O.
DO - 10.3390/molecules28135126
IS - 13
KW - Humanities
Humanism
Humans
Breast Neoplasms
Oxidative Stress
Inflammation
PY - 2023
SN - 1420-3049
ST - Green Synthesised TiO2 Nanoparticles-Mediated Terenna asiatica: Evaluation of
Their Role in Reducing Oxidative Stress, Inflammation and Human Breast Cancer
Proliferation
T2 - MOLECULES
TI - Green Synthesised TiO2 Nanoparticles-Mediated Terenna asiatica: Evaluation of
Their Role in Reducing Oxidative Stress, Inflammation and Human Breast Cancer
Proliferation
VL - 28
Y2 - 7
ID - 9474
ER -
TY - JOUR
AB - Curcuma Tonga (turmeric) has a long history of use in medicine as a treatment
for inflammatory conditions. The primary active constituent of turmeric and the one
responsible for its vibrant yellow color is curcumin. Curcumin is used for
treatment of wound and inflammation. It had antimicrobial and antioxidant property.
It has low intrinsic toxicity and magnificent properties like with comparatively
lesser side-effects. Cotton cloth is one of the most successful wound dressings
which utilize the intrinsic properties of cotton fibers. Modern wound dressings,
however, require other properties such as antibacterial and moisture maintaining
capabilities. In this study, conventional cotton cloth was coated with Curcumin
composite for achieving modern wound dressing properties. Curcumin nanocomposite is
characterized. The results show that coated cotton cloth with Curcumin
nanocomposite has increased drying time (74%) and water absorbency (50%).
Furthermore, they show antibacterial efficiency against bacterial species present
in wounds. (C) 2017 Elsevier B.V. All rights reserved.
AN - rayyan-553781537
AU - Venkatasubbu, G. D.
AU - Anusuya, T.
DO - 10.1016/j.ijbiomac.2017.02.002
KW - Bandages
PY - 2017
SN - 0141-8130 1879-0003
SP - 366-378
ST - Investigation on Curcumin nanocomposite for wound dressing
TI - Investigation on Curcumin nanocomposite for wound dressing
VL - 98
Y2 - 5
ID - 9475
ER -
TY - CONF
AB - Silver nanoparticles were found to have an excellent antimicrobial activity
and green synthesis enhance the activity of Silver nanoparticles. Mimosa pudica
commonly called as touch me-not plant has found to have antimicrobial, anti-
oxidant, anti-inflammatory and antiseptic properties. Ormocarpum cochinchinense
commonly called as 'elumbotti' is a shrub which is traditionally used for bone
fixing in the areas like Tamilnadu.Hydroxyapatite is one of the popular biomaterial
which has good biocompatibility commonly used in the field of dentistry and
Orthopaedics. Osteosarcoma or Osteogenic sarcoma is a primary malignant bone tumour
commonly affects children and adolescents. Recently, Hydroxyapatite was found to
have proliferative-suppressive effect on osteosarcoma cells. The leaves of Mimosa
pudica and ormocarpum cochin chinense were shadow dried and its aqueous extract was
prepared. The Silver nano particles were synthesized from both the plants and the
UV-visible spectrum shows good stability in mimosa pudica sample which was taken
for further use. Further the sample was characterized by Transmission Electron
Microscopy (TEM) and Energy dispersive X-ray analysis (EDX), X-ray Diffraction
(XRD).To enhance the drug delivery, synthesized silver nanoparticles were
conjugated with hydroxyapatite powder. The antimicrobial and cytotoxic activity of
the bio-conjugate was evaluated. The in-vitro activity of the bio conjugate will be
evaluated in the osteo sarcoma cell line (MG-63). The produced bio conjugate can be
used in the treatment of osteo sarcoma and in treating bone injuries and bone
inflammations. © 2022 Author(s).
AN - rayyan-553781538
AU - Vennila Preethi, S.
AU - Geetha Gayathri, V.
AU - Jeffrey Calwin, J.
DA - 2022
DO - 10.1063/5.0105011
KW - Cytotoxicity
Hydroxyapatite
Mimosa pudica
Osteosarcoma cell line (MG-63)
Durapatite
Tocopherols
ST - Synthesis of silver nanoparticles from mimosa pudica and bio-conjugation with
hydroxyapatite for orthopaedic application
T2 - AIP Conference Proceedings
TI - Synthesis of silver nanoparticles from mimosa pudica and bio-conjugation with
hydroxyapatite for orthopaedic application
85140330764&doi=10.1063%2f5.0105011&partnerID=40&md5=a4bb94acfeb6ec8e74d1697fd77087
29
VL - 2518
ID - 9476
ER -
TY - JOUR
AB - Zinc and its derivatives requirement increased to enhance human immunity
against the different pandemics, including covid-19. Green synthesis is an emerging
field of research. Zinc oxide (ZnO) nanoparticles have been prepared from
Anoectochilus elatus and characterized using absorption, vibrational and electron
microscope analysis. They were carried for antibacterial, inflammatory control
tendency, and potential antioxidant activities. The brine shrimp lethal assay
tested the biologically derived nanomaterial toxicity and the lethal concentration
(LC50) is 599.79 mu g/ml. The inhibition against the important disease-causing
pathogens was measured against four-gram negative, gram-positive bacteria and two
fungus pathogens. The nanomaterial exposed inhibition zone for gram-positive
bacteria between 17 mm and 25 mm. The inhibition zone against gram-negative
bacteria exists between 19 mm and 24 mm. The anti-inflammatory activity was
assessed by inhibition of protein denaturation and protease inhibitory activity
using nanomaterial. The antioxidant activity was examined using four assays for the
therapeutic activities. The average size range of 60-80 nm nanoparticles has
prepared and exposed the good biological activity between 50 mu g/ml and 100 mu
g/ml. The comparative results of anti-inflammatory and antioxidant assay results
with standards such as Aspirin and vitamin C exposed that two to three times higher
concentrations are required for the fifty percent of inhibitions. The prepared low-
cost nanoparticle has exhibited excellent biological activity without any side
effects and may enhance immunity. (C) 2021 The Author(s). Published by Elsevier
B.V. on behalf of King Saud University.
AN - rayyan-553781545
AU - Vijayakumar, N.
AU - Bhuvaneshwari, V. K.
AU - Ayyadurai, G. K.
AU - Jayaprakash, R.
AU - Gopinath, K.
AU - Nicoletti, M.
AU - Alarifi, S.
AU - Govindarajan, M.
DO - 10.1016/j.sjbs.2021.11.065
IS - 4
KW - Zinc
PY - 2022
SN - 1319-562X 2213-7106
SP - 2270-2279
ST - Green synthesis of zinc oxide nanoparticles using Anoectochilus elatus, and
their biomedical applications
TI - Green synthesis of zinc oxide nanoparticles using Anoectochilus elatus, and
their biomedical applications
VL - 29
Y2 - 4
ID - 9483
ER -
TY - JOUR
AB - Facile and low cost garlic clove extract based silver nanoparticles was
synthesized and its broad spectrum of therapeutic activity including antibiofilm,
antiparasitic and anti-breast cancer activity was evaluated. The synthesized
garlic-silver nanoparticles (G-AgNPs) were characterized by various physico-
chemical techniques. G-AgNPs showed good optical property, highly crystalline
nature, spherical shape and uniformly dispersed with size measuring between 10 and
50 nm. G-AgNPs have shown greater anti-bacterial and antibiofilm activity on
clinically important pathogens methicillin-resistant S. aureus and P. aerigunosa at
100 μg ml(-1). The efficacy of G-AgNPs against earthworm evidenced its effectiveness
as anti-helminthic agent in treating intestinal parasites. The significant
inhibition of BSA protein denaturation proves its anti-inflammatory property. In
addition, G-AgNPs have shown remarkable anticancer effect and significantly
inhibited the human breast cancer cell (MCF-7) viability at 100 μg ml(-1) after
24 h. A noticeable change in the morphology of MCF-7 cells was also noticed. G-
AgNPs were non-toxic to human HEK293 embryonic cells. Also, the non-toxic nature of
G-AgNPs to C. cornuta and no morphological, physiological changes proved its safety
to the environment. It is concluded that G-AgNPs have a broad range of biological
applications and it can be used as an eco-friendly material without having negative
effects in the environment.
AN - rayyan-553781878
AU - Vijayakumar, S.
AU - Malaikozhundan, B.
AU - Saravanakumar, K.
AU - Durán-Lara, E. F.
AU - Wang, M. H.
AU - Vaseeharan, B.
DO - 10.1016/j.jphotobiol.2019.111558
J2 - J Photochem Photobiol B
KW - Animals
Anthelmintics/*chemistry/pharmacology
Anti-Bacterial Agents/*chemistry/pharmacology
Anti-Inflammatory Agents/*chemistry/pharmacology
Antineoplastic Agents/*chemistry/pharmacology
Biofilms/drug effects
Cell Line, Tumor
Garlic/*chemistry/metabolism
HEK293 Cells
Humans
Metal Nanoparticles/*chemistry/toxicity
Methicillin-Resistant Staphylococcus aureus/drug effects
Oligochaeta/drug effects
Particle Size
Plant Extracts/chemistry
Protein Denaturation/drug effects
Pseudomonas aeruginosa/physiology
Silver/*chemistry
Garlic
LA - eng
N1 - Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology
in Animal Health Lab, Department of Animal Health and Management, Science campus
6th floor, Alagappa University, Karaikudi 630004, Tamil Nadu, India; Natural
product Biomedical Technology Laboratory, Department of Medical Biotechnology,
College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do
24341, Republic of Korea.; Nanobiosciences and Nanopharmacology Division,
Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health
and Management, Science campus 6th floor, Alagappa University, Karaikudi 630004,
Tamil Nadu, India; Department of Biology, The Gandhigram Rural Institute (Deemed to
be University), Gandhigram-624 302, Dindigul District, Tamil Nadu, India.; Natural
product Biomedical Technology Laboratory, Department of Medical Biotechnology,
College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do
24341, Republic of Korea.; Bio & NanoMaterials Lab, Drug Delivery and Controlled
Release, Universidad de Talca, Talca 3460000, Maule, Chile; Departamento de
Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca
3460000, Maule, Chile.; Natural product Biomedical Technology Laboratory,
Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon
National University, Chuncheon, Gangwon-do 24341, Republic of Korea. Electronic
address: mhwang@kangwon.ac.kr.; Nanobiosciences and Nanopharmacology Division,
Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health
and Management, Science campus 6th floor, Alagappa University, Karaikudi 630004,
Tamil Nadu, India. Electronic address: vaseeharanb@alagappauniversity.ac.in.
PY - 2019
SP - 111558
ST - Garlic clove extract assisted silver nanoparticle - Antibacterial,
antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity
assessment
T2 - Journal of photochemistry and photobiology. B, Biology
TI - Garlic clove extract assisted silver nanoparticle - Antibacterial,
antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity
assessment
VL - 198
Y2 - 9
ID - 9802
ER -
TY - JOUR
AB - Kappa-Carrageenan wrapped ZnO nanoparticles (KC-ZnO NPs) was synthesized,
physico-chemically characterized and evaluated their biocompatibility and
antimicrobial therapy against MRSA. XRD showed the highly crystalline and hexagonal
phase structure of ZnO NPs. FETEM confirmed the spherical and hexagonal shaped
particle with the mean size of 97.03 +/- 9.05 nm. The synthesized KC-ZnO NPs
exhibited significant antibacterial activity against MRSA. The biofilm growth of
MRSA was greatly inhibited at 100 mu g/ml as observed through live and dead cell
assay. KC-ZnO NPs have shown invitro anti-inflammatory activity (82%) at 500 mu
g/ml. KC-ZnO NPs was non-toxic to NIH3T3 mouse embryonic fibroblasts cell lines.
Further, no apoptotic and necrotic mediated death in NIH3T3 mouse embryonic
fibroblasts cells were noticed by flow cytometric analysis. KC-ZnO NPs have good
biocompatibility as recorded by the least hemolysis percentage (<3%) up to 100 mu
g/ml, which is much lesser than the acceptable limit. In addition, ecosafety
analysis has shown that KC-ZnO NPs and kappa karrageenan (0-500 mu g/ml) caused no
mortality of A. salina after 48 h. However, bare zinc acetate has shown 35%
mortality of A. salina after 48 h. The results conclude that KC-ZnO NPs could be a
novel antibacterial therapy for the treatment of MRSA associated infectious. (C)
2019 Elsevier B.V. All rights reserved.
AN - rayyan-553781546
AU - Vijayakumar, S.
AU - Saravanakumar, K.
AU - Malaikozhundan, B.
AU - Divya, M.
AU - Vaseeharan, B.
AU - Duran-Lara, E. F.
AU - Wang, M. H.
DO - 10.1016/j.ijbiomac.2019.12.030
KW - Biopolymers
Carrageenan
PY - 2020
SN - 0141-8130 1879-0003
SP - 9-18
ST - Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles
for anti MRSA therapy
TI - Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles
for anti MRSA therapy
VL - 144
Y2 - 2 y3 - 1
ID - 9484
ER -
TY - JOUR
AB - Currently, diabetes mellitus (DM) accelerated diabetic foot ulcer (DFU)
remains vivacious health problem related with delayed healing and high amputation
rates which leads to enormous clinical obligation. Keeping in view of the
foregoing, researchers have been made in their efforts to develop novel materials
which accelerate delayed wound healing in the diabetic patient and reduce the
adversative influences of DFUs. The most prominent materials used for the wound
healing application have biocompatibility, low cytotoxicity, excellent
biodegradable properties, and antimicrobial activity properties. Utilization of
nanoparticles has emerged as a protruding scientific and technological revolution
in controlling DFUs. Biopolymers in combination with bioactive nanoparticles having
antimicrobial, antibacterial, and anti-inflammatory properties have great potential
in wound care to enhance the healing process of diabetic wound infectious.
Combination of antibacterial nanoparticles like silver nanoparticles (AgNPs), gold
nanoparticles (AuNPs), copper nanoparticles (CuNPs) etc. with polymeric matrix
could efficiently inhibit bacterial growth and at the same time fastens the healing
process of a wound. This review briefed the recent development of different natural
polymers and antibacterial nanoparticles to mitigate the diabetes mellitus based
DFU. © 2018
AN - rayyan-553781547
AU - Vijayakumar, V.
AU - Samal, S. K.
AU - Mohanty, S.
AU - Nayak, S. K.
DO - 10.1016/j.ijbiomac.2018.10.120
KW - Diabetes
Nanoparticle
Wound healing
Animals
Biopolymers
Diabetes Mellitus
Humans
Metal Nanoparticles
Wound Healing
alginic acid
antiinfective agent
cellulose
chitosan
collagen
copper nanoparticle
dextran
elastin
gentamicin
gold nanoparticle
nanoparticle
silver nanoparticle
starch
unclassified drug
yttrium oxide nanoparticle
biopolymer
metal nanoparticle
bacterial growth
carbohydrate metabolism
cerebrovascular accident
circulation
cytotoxicity
diabetic neuropathy
diabetic retinopathy
enzyme inhibition
human
immune deficiency
insulin dependent diabetes mellitus
ischemic heart disease
nonhuman
pancreas islet beta cell
pathogenesis
peripheral vascular disease
Review
wound care
wound healing
animal
chemistry
diabetes mellitus
drug effect
pathophysiology
PY - 2019
SP - 137-148
ST - Recent advancements in biopolymer and metal nanoparticle-based materials in
diabetic wound healing management
TI - Recent advancements in biopolymer and metal nanoparticle-based materials in
diabetic wound healing management
85055679345&doi=10.1016%2fj.ijbiomac.2018.10.120&partnerID=40&md5=d34c4a2df73418c53
50b04ca3185c45c
VL - 122
ID - 9485
ER -
TY - JOUR
AB - Few studies have been conducted to evaluate the cellular composition of the
granulomatous lesions induced by Lacazia loboi. Thus, the objective of the present
study was to characterize the mononuclear cell population present in cutaneous
lesions obtained from 15 patients with Jorge Lobo's disease. Histological sections
were stained with hematoxylin-eosin and methenamine silver and the following
mononuclear cells were identified by immunohistochemistry: T lymphocytes (CD3+),
helper T lymphocytes (CD4+), cytotoxic T lymphocytes (CD8+), B lymphocytes (CD20+),
plasma cells (CD79+), natural killer cells (CD57+) and histiocytes (CD68+). This
study showed that the inflammatory infiltrate mainly consists of histiocytes and
multinucleated giant cells, in addition to the presence of a large number of fungal
cells. The identified inflammatory cells showed the following frequency: CD68+
histiocytes > CD3+ T lymphocytes > CD4+ T > CD8+ T lymphocytes > CD57+ natural
killer cells > CD79+ plasma cells > CD20+ B lymphocytes. Based on the findings of a
large number of fungal cells in the infected tissues and the disorganized cell
arrangement in the granuloma, we hypothesize that patients with Jorge Lobo's
disease present immunoregulatory disturbances, which are likely to be specific and
perhaps responsible for the lack of containment of the pathogen.
AN - rayyan-553782134
AU - Vilani-Moreno, F. R.
AU - Belone, A. F.
AU - Soares, C. T.
AU - Opromolla, D. V.
DO - 10.1016/s1130-1406(05)70006-1
IS - 1
J2 - Rev Iberoam Micol
KW - Antigens, CD/analysis
Brazil
Dermatomycoses/immunology/microbiology/*pathology
Giant Cells/*pathology
Granuloma/immunology/microbiology/*pathology
Host-Parasite Interactions
Humans
Killer Cells, Natural/pathology
Lymphocyte Subsets/*pathology
Male
Middle Aged
Mitosporic Fungi/immunology/*isolation & purification
Onygenales/immunology/*isolation & purification
Plasma Cells/*pathology
Skin/pathology
T-Lymphocyte Subsets/pathology
LA - eng
N1 - Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru-SP, Brazil.
imunologia@ilsl.br
PY - 2005
SN - 1130-1406 (Print)
SP - 44-9
ST - Immunohistochemical characterization of the cellular infiltrate in Jorge
Lobo's disease
T2 - Revista iberoamericana de micologia
TI - Immunohistochemical characterization of the cellular infiltrate in Jorge
Lobo's disease
VL - 22
Y2 - 3
ID - 10045
ER -
TY - JOUR
AB - During the last few decades, gold nanoparticles (AuNP's) have gained
considerable attention in nanomedicine and expanded its application in clinical
diagnosis and as therapeutics. Employing plant extract for synthesising gold
nanoparticles proves to be an eco-friendly technology for large scale production.
It is highly economical and suitable for biological applications by negating the
use of chemicals involved in conventional route. In this study, AuNP's was prepared
by a simple one step method of employing aqueous Mangifera indica seed extract as a
reducing agent. Scanning electron microscopy and transmission electron microscopy
revealed spherical shaped nanoparticles and dynamic light scattering analysis
indicated the AuNP's to be approximately 46.8 nm in size. AuNP's efficiently
inhibited the growth of E. coll. and S. aureus by its inherent ability to generate
reactive oxygen species (ROS) and exhibited detrimental effects towards the tested
bacterial species. Biocompatibility assessment indicated the non-toxic nature of
AuNP's towards mesenchymal stem cells at 25 mu g/ml and interestingly, suppressed
the growth of human gastric cancer cells under in vitro culture conditions. AuNP's
significantly exhibited anti-angiogenic property in chick chorioallantoic membrane
model (CAM) by downregulating Ang-1/Tie2 pathway. Overall, the synthesized AuNP's
exhibited antibacterial and anti-angiogenic properties with high biocompatibility
thereby supporting its candidature for various biomedical applications. It can be
employed in suppressing tumor growth, combat inflammatory diseases that necessitate
the involvement of angiogenesis suppression, and antibacterial activity is suitable
for its clinical translation to negate surgery associated infections.
AN - rayyan-553781548
AU - Vimalraj, S.
AU - Ashokkumar, T.
AU - Saravanan, S.
DO - 10.1016/j.biopha.2018.05.151
PY - 2018
SN - 0753-3322 1950-6007
SP - 440-448
ST - Biogenic gold nanoparticles synthesis mediated by Mangifera indica seed
aqueous extracts exhibits antibacterial, anticancer and anti-angiogenic properties
TI - Biogenic gold nanoparticles synthesis mediated by Mangifera indica seed
aqueous extracts exhibits antibacterial, anticancer and anti-angiogenic properties
VL - 105
Y2 - 9
ID - 9486
ER -
TY - JOUR
AB - Titanium dioxide nanoparticle (TNP) has been suggested for use in fish farms
to prevent or alleviate bacterial diseases owing to its bactericidal property.
Unfortunately, the interaction of TNP with cells impaired the host defenses of fish
resulting in increased mortality during bacterial challenges. The present study
evaluated the efficacy of the ethanolic extract of Tinospora cordifolia (TCE) as a
dietary supplement in ameliorating TNP induced toxicity in Nile tilapia
(Oreochromis niloticus). The fishes were exposed to environmentally relevant
concentration (10 mg/L) of TNP for 14 days and the effect of TCE supplemented feed
at 3 different doses (5, 10, and 15 g/kg) was studied. TCE signally increased the
weight gain, specific growth rate, and decreased feed conversion ratio in fish. TCE
significantly (P < 0.05) ameliorated the toxic effects caused by TNP by increasing
the antioxidant (CAT, SOD, GPx) activity and decreasing the levels of serum enzymes
(ALT, AST, ALP, ACP), macromolecular oxidation, excessive ROS production, and pro-
inflammatory cytokines (IL-1 beta, IL-6, IL-8, INF-gamma, TNF-alpha, PGE-2). TNP
bioaccumulation and histopathological alterations in gill, liver, and kidney were
also significantly alleviated by TCE supplementation. TCE perceptibly regulated the
expression of heat shock proteins (HSP60, -70), MAPKs (pERK1/2, pp38), antioxidant
(NRF2, Keap1, HO-1), apoptotic (p53, PDRG1), and antiapoptotic (AKT, Bcl2) proteins
in fish. Regarding disease resistance, the TCE co-treated groups showed reduced
cumulative mortality and higher relative percent survival with A. hydrophila. Our
results suggest that TNPinduced apoptosis is mediated by the MAPK/NRF2/Keap1
pathway and underlines the therapeutic potential of TCE in aqua-farming.
AN - rayyan-553781549
AU - Vineetha, V. P.
AU - Devika, P.
AU - Prasitha, K.
AU - Anilkumar, T. V.
DO - 10.1016/j.cbpc.2020.108908
KW - Titanium
Signal Transduction
Oxidative Stress
PY - 2021
SN - 1532-0456 1878-1659
ST - Tinospora cordifolia ameliorated titanium dioxide nanoparticle-induced
toxicity via regulating oxidative stress-activated MAPK and NRF2/Keap1 signaling
pathways in Nile tilapia (Oreochromis niloticus)
T2 - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
TI - Tinospora cordifolia ameliorated titanium dioxide nanoparticle-induced
toxicity via regulating oxidative stress-activated MAPK and NRF2/Keap1 signaling
pathways in Nile tilapia (Oreochromis niloticus)
VL - 240
Y2 - 2
ID - 9487
ER -
TY - JOUR
AB - Background: Syzygium aromaticum, also known as clove, and its essential oil
has already been proved to have antioxidant, anti-inflammatory and anticancer
properties. Clove is used in various foods owing to its potent antimicrobial and
antioxidant properties. Essential oil extractedfrom clove has been used in
traditional medicine for treating various ailments. Methods: In silico analyses of
phytocompounds of Syzygium aromaticum namely eugenol, B-caryophyllene, gallic acid,
crategolic acid, kaempferol, quercetin, cinnamaldehyde, and oleanolic acid were
docked with three apoptotic proteins involved in breast cancer, namely BCL-2, BAX
and APAF-1 using AUTODOCK. In addition, flower bud extract of Syzygiumaromaticumwas
used for the synthesis of AgNPs (silver nanoparticles). The synthesized clove-
silver nanoparticles were then characterized using various techniques such as
Ultraviolet-visible spectrophotometry, FTIR, FESEM-EDX, DLS and zeta potential to
determine the particle size, shape, crystalline structure, and stability of CL-
AgNPs and tested for its anticancer potential in MCF-7 cell lines. Results: In
silico analysis predicted that phytochemicals of clove have good interactions with
the apoptosis related proteins of breast cancer. In vitro assay confirmed the
cytotoxic effect of the synthesized CL-AgNPs on breast cancer cells using the MCF-7
cell line with the IC50 value of 58.64 µg/ml. Conclusion: In vitro analysis of the
anticancer activity of CL-AgNPs in MCF-7 cell line supports the in silico study by
proving active interactions between the phytochemicals of clove and target proteins
of the breast cancer and hence Syzygium aromaticum has been proved to possess
potential anticancer property. Further research is needed to consider clove-silver
nanoparticles as a novel drug for treating breast cancer. © 2023 Universidad de los
Andes, Bogota Colombia. All rights reserved.
AN - rayyan-553781550
AU - Vishal, J.
AU - Ranjani, S.
AU - Karunya, R. J.
AU - Hemalatha, S.
DO - 10.32768/abc.2023103291-300
IS - 3
KW - Anticancer agent
Antioxidant
Breast cancer
Metallic nanoparticle
Molecular docking
Syzygium
Antioxidants
PY - 2023
SP - 291-300
ST - Synthesis, Characterization and Evaluation of Antioxidant, Anticancer and
Toxicity Properties of Silver Nanoparticles Synthesized From Syzygium Aromaticum
T2 - Archives of Breast Cancer
TI - Synthesis, Characterization and Evaluation of Antioxidant, Anticancer and
Toxicity Properties of Silver Nanoparticles Synthesized From Syzygium Aromaticum
85168091774&doi=10.32768%2fabc.2023103291-
300&partnerID=40&md5=6e9ad4c64b453ec1d95d72baaf1b5af5
VL - 10
ID - 9488
ER -
TY - JOUR
AB - Leishmaniasis is endemic to the tropical and subtropical regions of the world
and is transmitted by the bite of an infected sand fly. The multifaceted
interactions between Leishmania, the host innate immune cells, and the adaptive
immunity determine the severity of pathogenesis and disease development. Leishmania
parasites establish a chronic infection by subversion and attenuation of the
microbicidal functions of phagocytic innate immune cells such as neutrophils,
macrophages and dendritic cells (DCs). Other innate cells such as inflammatory
monocytes, mast cells and NK cells, also contribute to resistance and/or
susceptibility to Leishmania infection. In addition to the cytokine/chemokine
signals from the innate immune cells, recent studies identified the subtle shifts
in the metabolic pathways of the innate cells that activate distinct immune signal
cascades. The nexus between metabolic pathways, epigenetic reprogramming and the
immune signaling cascades that drive the divergent innate immune responses, remains
to be fully understood in Leishmania pathogenesis. Further, development of safe and
efficacious vaccines against Leishmaniasis requires a broader understanding of the
early interactions between the parasites and innate immune cells. In this review we
focus on the current understanding of the specific role of innate immune cells, the
metabolomic and epigenetic reprogramming and immune regulation that occurs during
visceral leishmaniasis, and the strategies used by the parasite to evade and
modulate host immunity. We highlight how such pathways could be exploited in the
development of safe and efficacious Leishmania vaccines.
AN - rayyan-553781759
AU - Volpedo, G.
AU - Pacheco-Fernandez, T.
AU - Oljuskin, T.
AU - Dey, R.
AU - Gannavaram, S.
AU - Satoskar, A. R.
AU - Nakhasi, H. L.
DO - 10.3389/fimmu.2021.748325
J2 - Front Immunol
KW - Animals
Cytotoxicity, Immunologic
Dendritic Cells/immunology
Humans
Immune Evasion
*Immunity, Innate
Immunogenicity, Vaccine
Killer Cells, Natural/immunology
Leishmania donovani/*immunology
Leishmaniasis Vaccines/*immunology
Leishmaniasis, Visceral/*immunology
Macrophages/immunology/parasitology
Mast Cells/immunology
Metabolomics
Mice
Mice, Inbred C57BL
Monocytes/immunology
Natural Killer T-Cells/immunology
Neutrophils/immunology
*Vaccine Development
Vaccination
LA - eng
N1 - Departments of Pathology and Microbiology, Wexner Medical Center, The Ohio
State University, Columbus, OH, United States.; Departments of Pathology and
Microbiology, Wexner Medical Center, The Ohio State University, Columbus, OH,
United States.; Laboratory of Emerging Pathogens, Division of Emerging and
Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research,
Food and Drug Administration, Silver Spring, MD, United States.; Laboratory of
Center for Biologics Evaluation and Research, Food and Drug Administration, Silver
Spring, MD, United States.; Laboratory of Emerging Pathogens, Division of Emerging
and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research,
Food and Drug Administration, Silver Spring, MD, United States.; Laboratory of
Spring, MD, United States.; Departments of Pathology and Microbiology, Wexner
Medical Center, The Ohio State University, Columbus, OH, United States.; Laboratory
of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases,
Spring, MD, United States.
PY - 2021
SP - 748325
ST - Determinants of Innate Immunity in Visceral Leishmaniasis and Their
Implication in Vaccine Development
T2 - Frontiers in immunology
TI - Determinants of Innate Immunity in Visceral Leishmaniasis and Their
Implication in Vaccine Development
VL - 12
ID - 9692
ER -
TY - JOUR
AB - 1. Sulindac, cis-5-fluoro-2-methyl-1-(p-methylsulphinylbenzylidene)indene-3-
acetic acid, inhibits growth of colon polyps and cancers. This effect has been
attributed to inhibition of prostaglandin synthesis but more recent observations
indicate that, in vitro, cells that do not have cyclo-oxygenase nor RNA for
synthesis of such enzymes are affected by sulindac. Therefore the presumptive
effect is probably not correct. 2. It has also been found that sulindac stimulates
apoptosis. It is herein postulated that in tumour cells such effects may be due to
interaction of the anionic form of the drug with protons in the intermembrane space
of mitochondria to disrupt the potential across the inner mitochondrial membrane
and thereby initiate apoptosis. Normal cells are not affected.
AN - rayyan-553781551
AU - Waddell, W. R.
DO - 10.1042/CS19970251
IS - 3
KW - Apoptosis
Piroxicam
Sulindac
PY - 1998
SN - 0143-5221
SP - 385-388
ST - Stimulation of apoptosis by sulindac and piroxicam
T2 - CLINICAL SCIENCE
TI - Stimulation of apoptosis by sulindac and piroxicam
VL - 95
Y2 - 9
ID - 9489
ER -
TY - JOUR
AB - Regarding applicative, facile, green chemical research, a bio-inspired
approach is being reported for the synthesis of Au nanoparticles by pectin (PEC) as
a natural reducing and stabilizing agent without using any toxic and harmful
reagent under ultrasonic condition. The biosynthesized Au NPs@PEC were
characterized by advanced physicochemical techniques like ultraviolet-visible (UV-
Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron
Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray
spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that
pectin-stabilized Au nanoparticles have a spherical shape with a mean diameter from
5 to 10 nm. In the medicinal part of the present research, the lung BEAS-2B, WI-38,
CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell viability was determined by trypan blue
assay. The caspase activity colorimetric assay kit and Rhodamine123 fluorescence
dye were used to determine the caspase-3 activity and mitochondrial membrane
potential, respectively. Apoptosis and DNA fragmentation were determined by the
TUNEL test. Also, the inflammatory cytokines concentrations were evaluated by the
Rat inflammatory cytokine assay kit. Au NPs@PEC-treated cell cutlers decreased
significantly (p < 0.01) the caspase-3 activity, inflammatory cytokines
concentrations, and DNA fragmentation, and enhanced the mitochondrial membrane
potential and cell viability in the high concentration of Methotrexate-treated lung
BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cells. In the antioxidant
test, the IC50 of Au NPs@PEC nanocomposite and BHT against DPPH free radicals were
203 and 181 mg/mL, respectively. Finally, Au NPs@PEC may be used as a pulmonary
protective supplement to treat acute lung injury. (c) 2021 The Authors. Published
by Elsevier B.V. on behalf of King Saud University. This is an open access article
under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - rayyan-553781554
AU - Wang, D. X.
AU - Pu, J.
AU - Liao, Y. B.
AU - Liu, J. H.
AU - Hu, G.
DO - 10.1016/j.arabjc.2021.103533
IS - 1
KW - Lung
Cell Line
PY - 2022
SN - 1878-5352 1878-5379
ST - Pulmonary protective effects of ultrasonic green synthesis of gold
nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung
BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
TI - Pulmonary protective effects of ultrasonic green synthesis of gold
nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung
BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
VL - 15
Y2 - 1
ID - 9492
ER -
TY - JOUR
AB - Background: 5-demethylnobiletin is a natural polymethoxyflavone which is
isolated from the extract of citrus fruits peels. It exhibits a broad spectrum of
biological activities such as anti-cancer, anti-inflammatory, cardiovascular
protective and neuroprotective effects, however, its effect in melanogenesis
remains uninvestigated. Purpose: Melanin synthesis is a very important biological
process in curing disease such as vitiligo with depigmentation on the skin. In the
current work, we aim to confirm the bioactivity and mechanism of 5-
demethylnobiletin in stimulating melanogenesis. Study Design: To confirm the
mechanistic role of 5-demethylnobiletin in enhancing melanogenesis, its effect on
the activity of tyrosinase, together with the level of microphthalmia-associated
transcription factor (MITF), Trp-1, Trp-2, melanocyte-specific marker protein
PMEL17, Rab27a, Melanophilin and Myosin VA were studied in B16F10 melanoma cells.
Methods: Multiple biological assays on melanogenesis-associated proteins such as
melanin content detection, tyrosinase activity colorimetric assay, qPCR, western
blot analysis, dual-luciferase reporter assay, cAMP activity assay and Fontana-
Masson ammoniacal silver staining were used to confirm the role of 5-
demethylnobiletin in stimulating melanin synthesis and the transportation of
melanosomes. Results: As confirmed by multiple biological assays, 5-
demethylnobiletin is found to stimulate dendrite structure formation in cells,
melanin synthesis and the transportation of melanosomes, via inducing the
phosphorylation of cAMP response element-binding protein (CREB) and increasing the
intracellular levels of cAMP in vitro through the PKA-dependent pathway.
Conclusion: The findings suggested that 5-demethylnobiletin may be considered as a
potential natural product candidate for patients with pigment disorder. © 2022
Elsevier GmbH
AN - rayyan-553781557
AU - Wang, H. M.
AU - Qu, L. Q.
AU - Ng, J. P. L.
AU - Zeng, W.
AU - Yu, L.
AU - Song, L. L.
AU - Wong, V. K. W.
AU - Xia, C. L.
AU - Law, B. Y. K.
DO - 10.1016/j.phymed.2022.153941
KW - 5-demethylnobiletin
B16F10
cAMP/CREB pathway
Melanogenesis
5 demethylnobiletin
cyclic AMP
cyclic AMP responsive element binding protein
dopachrome tautomerase
flavanone
melanin
melanocyte protein Pmel 17
microphthalmia associated transcription factor
monophenol monooxygenase
myosin Va
Ras related protein Rab 27A
tyrosinase related protein 1
unclassified drug
animal cell
Article
B16-F10 cell line
bioassay
biological activity
cell activation
cell viability
Citrus
colorimetry
controlled study
dendrite
drug cytotoxicity
drug effect
drug mechanism
drug purity
drug structure
enzyme activity
in vitro study
luciferase assay
Masson staining
melanogenesis
melanosome
mouse
nonhuman
pigmentation
protein expression
signal transduction
silver staining
upregulation
Western blotting
PY - 2022
ST - Natural Citrus flavanone 5-demethylnobiletin stimulates melanogenesis through
the activation of cAMP/CREB pathway in B16F10 cells
T2 - Phytomedicine
TI - Natural Citrus flavanone 5-demethylnobiletin stimulates melanogenesis through
the activation of cAMP/CREB pathway in B16F10 cells
85123685096&doi=10.1016%2fj.phymed.2022.153941&partnerID=40&md5=b9c80718f17066f9e5e
96ddec552b608
VL - 98
ID - 9495
ER -
TY - JOUR
AB - Periodontitis noted as dental care disease, which occur the inflammatory
process in the teeth tissues surrounding which has the response to bacterial
accumulation. The main objectives of the present investigations were to improve the
multifunctional nanocomposite with PLGA@fluorapatite and enhance the bactericidal
inhibitory efficiency against periodontitis-related pathogens. The peptide modified
fluorapatite@PLGA nanocomposite were synthesized and physico-chemically
characterized. The observed analytical results demonstrated rod-like nanoparticles
formation with favorable human cell compatibility and toxicity against bacterial
pathogens. In addition, the use of FA and PLGA dual agents in the composite
achieved the greatest reduction in the biofilm growth and the metabolic activity of
polysaccharide production. The CFU count in the biofilms was reduced by nearly 3
orders for periodontal pathogens. Therefore, the new modified peptide PLGA-FA
composite promising in the root caries restorations to inhibit periodontitis
related pathogens.
AN - rayyan-553781558
AU - Wang, J. H.
AU - Li, Y.
AU - Jing, J.
AU - Yue, H. L.
AU - Zhang, L. L.
AU - Hua, W.
AU - Li, N.
AU - Liu, X.
AU - Han, J. A.
DO - 10.1088/2053-1591/abf2e8
IS - 5
KW - C-Peptide
Periodontics
PY - 2021
SN - 2053-1591
ST - Practical evaluations of bioactive peptide-modified Fluorapatite/PLGA
multifunctional nano-clustery composite against for root caries restorations to
inhibit periodontitis-related pathogens in periodontitis care
T2 - MATERIALS RESEARCH EXPRESS
TI - Practical evaluations of bioactive peptide-modified Fluorapatite/PLGA
multifunctional nano-clustery composite against for root caries restorations to
inhibit periodontitis-related pathogens in periodontitis care
VL - 8
Y2 - 5
ID - 9496
ER -
TY - JOUR
AB - The primary idea behind this study is to synthesize Saudi Origanum vulgare L.
extract mediated AuNPs and to investigate their efficacy in significantly treating
AMD. The biocompatible AuNPs were tested for their radical scavenging efficiency in
vitro. Also, The effects of AuNPs in suppressing the angiogenic protein expression
and inflammatory cytokine levels were analyzed in ARPE-19 (human retinal pigment
epithelium-19) as well as and human umbilical vein endothelium cell lines in vitro.
From the ORAC findings, it was evident that the bio synthesized AuNPs have
presented momentous anti-oxidative activity in reducing the apoptosis in H2O2
treated ARPE-19 cell lines. In addition, the biocompatible AuNPs exhibited
significant potential in suppressing the LPS stimulated inflammatory reaction in
ARPE-19 cell lines while demonstrating inhibitory expression of growth factor in
ARPE-19 as well as in umbilical vein endothelium cell lines. The experimental
findings in this study have suggested that the synthesized AuNPs are biocompatible
with significant potential in defending against angiogenesis, apoptosis, and
generation of pro-inflammatory cytokines in AMD disease.
AN - rayyan-553781559
AU - Wang, J.
AU - Wang, Y.
AU - Li, Q. M.
DO - 10.1016/j.jddst.2019.101377
KW - Macular Degeneration
PY - 2020
SN - 1773-2247
ST - Synthesis of AuNPs using plant polyphenols and their potential treatment for
age-related macular degeneration
TI - Synthesis of AuNPs using plant polyphenols and their potential treatment for
age-related macular degeneration
VL - 55
Y2 - 2
ID - 9497
ER -
TY - JOUR
AB - Purpose: To evaluate the adverse vascular effects of nanoparticles (NPs) in
vitro, extensive studies have investigated the toxicity of NPs on endothelial
cells, but the knowledge of potential toxicity on human smooth-muscle cells (SMCs)
is currently limited. Methods: This study compared the toxicity of TiO 2 , ZnO, and
Ag NPs to human aortic SMCs. Results: Only ZnO NPs significantly induced
cytotoxicity, accompanied by increased intracellular reactive oxygen species, Zn
ions, and endoplasmic reticulum stress biomarkers (DDIT3 expression and p-Chop
proteins). All the NPs significantly promoted the release of soluble VCAM1 and
soluble sICAM1, but not IL6, which suggested that metal-based NPs might promote
inflammatory responses. Furthermore, KLF4 expression (a transcription factor for
SMC-phenotype switch) was significantly induced by TiO 2 NPs and modestly by ZnO
NPs, but the expression of CD68 remained unaltered. Conclusion: Our data indicated
that ZnO NPs were more cytotoxic to human aortic SMCs than TiO 2 and Ag NPs at the
same mass concentrations, which might have been associated with intracellular
reactive oxygen species, Zn ions, and endoplasmic reticulum stress. © 2018 Wang et
al.
AN - rayyan-553781560
AU - Wang, M.
AU - Yang, Q.
AU - Long, J.
AU - Ding, Y.
AU - Zou, X.
AU - Liao, G.
AU - Cao, Y.
DO - 10.2147/IJN.S188175
KW - Cytotoxicity
Endoplasmic reticulum stress
ER stress
HASMCs
Human aortic smooth-muscle cells
Inflammation
Metal-based nanoparticles
NPs
Animals
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Aorta
Biomarkers
Cell Death
Cell Survival
Cytokines
Endocytosis
Endoplasmic Reticulum Stress
Humans
Ions
Kruppel-Like Transcription Factors
Metal Nanoparticles
Models, Biological
Myocytes, Smooth Muscle
Silver
Titanium
Zinc Oxide
biological marker
CD68 antigen
growth arrest and DNA damage inducible protein 153
kruppel like factor 4
silver nanoparticle
autacoid
CD68 antigen, human
cytokine
differentiation antigen
ion
kruppel like factor
leukocyte antigen
metal nanoparticle
silver
titanium
titanium dioxide
zinc oxide
antigen expression
aortic smooth muscle cell
Article
comparative study
controlled study
cytotoxicity
endoplasmic reticulum stress
gene switching
human
human cell
in vitro study
inflammation
nanotoxicology
protein expression
animal
aorta
biological model
cell death
cell survival
cytology
drug effect
endocytosis
metabolism
smooth muscle cell
ultrastructure
Humanities
Humanism
PY - 2018
SP - 8037-8049
ST - A comparative study of toxicity of TiO 2 , ZnO, and Ag nanoparticles to human
aortic smooth-muscle cells
T2 - International Journal of Nanomedicine
TI - A comparative study of toxicity of TiO 2 , ZnO, and Ag nanoparticles to human
aortic smooth-muscle cells
85058820081&doi=10.2147%2fIJN.S188175&partnerID=40&md5=a5d346c29356f5d025bf6d1540f3
bbc3
VL - 13
ID - 9498
ER -
TY - JOUR
AB - The current study aimed to explore the effects of supplementing paraformic
acid (PFA) into broilers’ diet on growth performance, inflammatory responses, and
liver protection. A total of 567 healthy one-day-old broilers were used in a 42-d
study, and they were randomized into three groups. Broilers were fed a basal diet
(CON group) or the basal diet supplemented with either 50 mg/kg aureomycin (AB
group) or 1000 mg/kg PFA (PFA group). The results showed that the PFA and AB groups
had a higher feed conversion rate than the CON group from day 21 to 42 (p < 0.05).
Dietary PFA or aureomycin supplementation decreased serum levels of interleukin
(IL)-1β, IL-6, IL-10, alanine transaminase, diamine oxidase, and D-lactate, and
significantly increased serum concentrations of immunoglobulin (Ig) A, IgM, and
complement C4 (p < 0.05). Moreover, dietary PFA or aureomycin supplementation
decreased hepatic levels of caspase-1, NOD-like receptor family pyrin domain
containing 3 (NLRP3), tumor necrosis factor-alpha, IL-6, and IL-18, as well as NF-
κB mRNA expression (p < 0.05). Above all, PFA supplementation into the broilers’
diet improved growth performance, inhibited inflammatory responses, and benefited
liver protection. The protective effects of PFA on the liver might be related to
inhibition of caspase-1-induced pyroptosis via inactivating the NF-κB/NLRP3
inflammasome axis in broiler chickens. © 2022 by the authors.
AN - rayyan-553781562
AU - Wang, Q.
AU - Niu, J.
AU - Liu, Y.
AU - Jiao, N.
AU - Huang, L.
AU - Jiang, S.
AU - Yan, L.
AU - Yang, W.
AU - Li, Y.
DO - 10.3390/ani12202825
IS - 20
KW - antibiotic growth promoter
chicken
feed additives
formic acid
liver
cadmium
microplastic
nanoplastic
polystyrene
silver nitrate
trypan blue
animal experiment
Article
blood cell
cell viability
comet assay
controlled study
cytotoxicity
DNA damage
Drosophila melanogaster
fluorescence intensity
genetic damage
genotoxicity
genotoxicity assay
health hazard
hemolymph
intestine cell
intestine injury
larva
nonhuman
oxidative stress
pollutant
stereomicroscopy
toxicity testing
trypan blue assay
Chickens
Liver
PY - 2022
ST - Supplementation of Paraformic Acid as a Substitute for Antibiotics in the
Diet Improves Growth Performance and Liver Health in Broiler Chickens
T2 - Animals
TI - Supplementation of Paraformic Acid as a Substitute for Antibiotics in the
Diet Improves Growth Performance and Liver Health in Broiler Chickens
85140398812&doi=10.3390%2fani12202825&partnerID=40&md5=ac49ac30d6d01876e6e61f2594c8
ba17
VL - 12
ID - 9500
ER -
TY - JOUR
AB - Co ions released due to corrosion of Co nanoparticles (CoNPs) in the
lysosomes of macrophages may be a factor in the particle-induced cytotoxicity and
aseptic inflammation accompanying metal-on-metal (MOM) hip prosthesis failure.
Here, we show that CoNPs are easily dissolved under a low pH, simulating the acidic
lysosomal environment. We then used bafilomycin A1 to change the pH inside the
lysosome to inhibit intracellular corrosion of CoNPs and then investigated its
protective effects against CoNP-induced cytotoxicity and aseptic inflammation on
murine macrophage RAW264.7 cells. XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-
[(phenylamino) carbonyl]-2H-tetrazolium hydroxide} assays revealed that bafilomycin
A1 can significantly decrease CoNP-induced cytotoxicity in RAW264.7 cells. Enzyme-
linked immunosorbent assays showed that bafilomycin A1 can significantly decrease
the subtoxic concentration of CoNP-induced levels of pro-inflammatory cytokines
(tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6), but has no
effect on anti-inflammatory cytokines (transforming growth factor-beta and
interleukin-10) in RAW264.7 cells. We studied the protective mechanism of
bafilomycin A1 against CoNP-induced effects in RAW264.7 cells by measuring
glutathione/oxidized glutathione (GSH/GSSG), superoxide dismutase, catalase, and
glutathione peroxidase levels and employed scanning electron microscopy,
transmission electron microscopy, and energy dispersive spectrometer assays to
observe the ultrastructural cellular changes. The changes associated with apoptosis
were assessed by examining the pAKT and cleaved caspase-3 levels using Western
blotting. These data strongly suggested that bafilomycin A1 can potentially
suppress CoNP-induced cytotoxicity and aseptic inflammation by inhibiting
intracellular corrosion of CoNPs and that the reduction in Co ions released from
CoNPs may play an important role in downregulating oxidative stress in RAW264.7
cells.
AN - rayyan-553781564
AU - Wang, S. H.
AU - Liu, F.
AU - Zeng, Z. X.
AU - Yang, H. L.
AU - Jiang, H. T.
DO - 10.1007/s12011-015-0381-9
IS - 1
KW - Inflammation
Macrophages
PY - 2016
SN - 0163-4984 1559-0720
SP - 94-105
ST - The Protective Effect of Bafilomycin A1 Against Cobalt Nanoparticle-Induced
Cytotoxicity and Aseptic Inflammation in Macrophages In Vitro
TI - The Protective Effect of Bafilomycin A1 Against Cobalt Nanoparticle-Induced
Cytotoxicity and Aseptic Inflammation in Macrophages In Vitro
VL - 169
Y2 - 1
ID - 9502
ER -
TY - JOUR
AB - Diabetic foot ulcer (DFU) is one of the most serious complication of diabetes
mellitus with the characteristic of long-term non-healing wound, which often leads
to amputation and eventual death. In response to the refractory wound, medical
dressings are considered helpful. However, traditional dressings often have the
disadvantages of limited efficacy and difficulty in long-term maintenance,
therefore there is an urgent need to develop novel dressing supplements. A variety
of biomaterials have been used as wound dressings to promote wound repair. In
particular, the injectable hydrogels with 3D structures similar to soft tissue have
excellent biocompatibility, which can fill the irregular wound to play the effect
of moisturizing, anti-infection, etc. On this basis, the injectable hydrogels can
also be loaded with carriers with certain physiological and pharmacological
effects, such as inorganic nanoparticles, drugs, and bioactive carriers, to achieve
functions of antibacterial, anti-inflammatory, antioxidant, promoting collagen
deposition, and vascular regeneration. Therefore they can promote wound healing
well and have a great prospect in wound dressing. This review describes the
mechanisms of DFU, summarizes the research progress of injectable carrier hydrogel
systems in DFU repair, evaluates the advantages and disadvantages of various
materials and the therapeutic principles of carriers, and proposes the challenges
and development prospects of injectable carrier hydrogel.
AN - rayyan-553781565
AU - Wang, S. W.
AU - Zhang, J.
AU - Zhou, W. L.
AU - Liu, W. T.
AU - Ou, Y.
AU - Zheng, X. X.
AU - Yang, H. Z.
AU - Wang, T. L.
DO - 10.1007/s10853-023-08730-x
IS - 28
KW - Diabetic Foot
PY - 2023
SN - 0022-2461 1573-4803
SP - 11441-11468
ST - Injectable carrier hydrogel for diabetic foot ulcer wound repair
T2 - JOURNAL OF MATERIALS SCIENCE
TI - Injectable carrier hydrogel for diabetic foot ulcer wound repair
VL - 58
Y2 - 7
ID - 9503
ER -
TY - JOUR
AB - The heart is an autoimmune-prone organ. It is crucial for the heart to keep
injury-induced autoimmunity in check to avoid autoimmune-mediated inflammatory
disease. However, little is known about how injury-induced autoimmunity is
constrained in hearts. Here, we reveal an unknown intramyocardial immunosuppressive
program driven by Tbx1, a DiGeorge syndrome disease gene that encodes a T-box
transcription factor (TF). We found induced profound lymphangiogenic and
immunomodulatory gene expression changes in lymphatic endothelial cells (LECs)
after myocardial infarction (MI). The activated LECs penetrated the infarcted area
and functioned as intramyocardial immune hubs to increase the numbers of
tolerogenic dendritic cells (tDCs) and regulatory T (Treg) cells through the
chemokine Ccl21 and integrin Icam1, thereby inhibiting the expansion of
autoreactive CD8(+) T cells and promoting reparative macrophage expansion to
facilitate post-MI repair. Mimicking its timing and implementation may be an
additional approach to treating autoimmunity-mediated cardiac diseases.
AN - rayyan-553782420
AU - Wang, W.
AU - Li, X.
AU - Ding, X.
AU - Xiong, S.
AU - Hu, Z.
AU - Lu, X.
AU - Zhang, K.
AU - Zhang, H.
AU - Hu, Q.
AU - Lai, K. S.
AU - Chen, Z.
AU - Yang, J.
AU - Song, H.
AU - Wang, Y.
AU - Wei, L.
AU - Xia, Z.
AU - Zhou, B.
AU - He, Y.
AU - Pu, J.
AU - Liu, X.
AU - Ke, R.
AU - Wu, T.
AU - Huang, C.
AU - Baldini, A.
AU - Zhang, M.
AU - Zhang, Z.
DO - 10.1016/j.immuni.2023.07.019
J2 - Immunity
KW - Myocardial Infarction
LA - eng
N1 - Pediatric Translational Medicine Institute and Pediatric Congenital Heart
Disease Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong
University School of Medicine, Shanghai 200127, China.; Gene Editing Laboratory,
The Texas Heart Institute, Houston, TX 77030, USA.; Pediatric Translational
Medicine Institute and Pediatric Congenital Heart Disease Institute, Shanghai
Children's Medical Center, Shanghai Jiao Tong University School of Medicine,
Shanghai 200127, China.; Pediatric Translational Medicine Institute and Pediatric
Congenital Heart Disease Institute, Shanghai Children's Medical Center, Shanghai
Jiao Tong University School of Medicine, Shanghai 200127, China.; Department of
Cardiovascular Surgery, Ninth People's Hospital, Shanghai Jiao Tong University
School of Medicine, Shanghai 200011, China.; Silver Snake (Shanghai) Medical
Science and Technique Co., Ltd., Shanghai 200030, China.; Department of
Anesthesiology, Shanghai Children's Medical Center, Shanghai Jiao Tong University
School of Medicine, Shanghai 200127, China.; Shanghai Institute of Immunology and
Department of Immunology and Microbiology, Key Laboratory of Cell Differentiation
and Apoptosis of Chinese Ministry of Education, Faculty of Basic Medicine, Shanghai
Jiao Tong University School of Medicine, Shanghai 200025, China.; Shanghai
Institute of Immunology and Department of Immunology and Microbiology, Key
Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education,
Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine,
Shanghai 200025, China.; Pediatric Translational Medicine Institute and Pediatric
Congenital Heart Disease Institute, Shanghai Children's Medical Center, Shanghai
Jiao Tong University School of Medicine, Shanghai 200127, China.; Pediatric
Translational Medicine Institute and Pediatric Congenital Heart Disease Institute,
Shanghai Children's Medical Center, Shanghai Jiao Tong University School of
Medicine, Shanghai 200127, China.; Pediatric Translational Medicine Institute and
Pediatric Congenital Heart Disease Institute, Shanghai Children's Medical Center,
Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.;
Pediatric Translational Medicine Institute and Pediatric Congenital Heart Disease
Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School
of Medicine, Shanghai 200127, China.; Pediatric Translational Medicine Institute
and Pediatric Congenital Heart Disease Institute, Shanghai Children's Medical
Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.;
Pediatric Translational Medicine Institute and Pediatric Congenital Heart Disease
Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School
of Medicine, Shanghai 200127, China.; Department of Neurosurgery, Shanghai
Shanghai 200127, China.; The State Key Laboratory of Cell Biology, CAS Center for
Excellence on Molecular Cell Science, Shanghai Institute of Biochemistry and Cell
Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences,
Shanghai 200031, China.; Cyrus Tang Hematology Center, Collaborative Innovation
Center of Hematology, State Key Laboratory of Radiation Medicine and Protection,
Soochow University, Suzhou 215123, China.; State Key Laboratory for Oncogenes and
Related Genes, Division of Cardiology, Renji Hospital, School of Medicine, Shanghai
Cancer Institute, Shanghai Jiao Tong University, Shanghai 200127, China.; BGI-
Shenzhen, Shenzhen 518083, China.; School of Medicine and School of Biomedical
Sciences, Huaqiao University, Quanzhou, Fujian 362021, China.; Shanghai
Collaborative Innovative Center of Intelligent Medical Device and Active Health,
Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.;
Shanghai Institute of Immunology and Department of Immunology and Microbiology, Key
Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education,
Faculty of Basic Medicine, Shan hai Jiao Tong University School of Medicine,
Shanghai 200025, China.; Institute of Genetics and Biophysics "ABT," CNR, Naples
80131, Italy; Department of Molecular Medicine and Medical Biotechnologies,
University of Naples, Federico II, Naples 80131, Italy.; Pediatric Translational
Medicine Institute and Pediatric Congenital Heart Disease Institute, Shanghai
Shanghai 200127, China. Electronic address: minzhang5099@gmail.com.; Pediatric
Translational Medicine Institute and Pediatric Congenital Heart Disease Institute,
Shanghai Children's Medical Center, Shanghai Jiao Tong University School of
Medicine, Shanghai 200127, China; Shanghai Collaborative Innovative Center of
Intelligent Medical Device and Active Health, Shanghai University of Medicine &
Health Sciences, Shanghai 201318, China. Electronic address: zhenzhang@sjtu.edu.cn.
PY - 2023
ST - Lymphatic endothelial transcription factor Tbx1 promotes an immunosuppressive
microenvironment to facilitate post-myocardial infarction repair
T2 - Immunity
TI - Lymphatic endothelial transcription factor Tbx1 promotes an immunosuppressive
microenvironment to facilitate post-myocardial infarction repair
Y2 - 8 y3 - 15
ID - 10327
ER -
TY - JOUR
AB - Nanocellulose is increasingly considered for applications; however, the
fibrillar nature, crystalline phase, and surface reactivity of these high aspect
ratio nanomaterials need to be considered for safe biomedical use. Here a
comprehensive analysis of the impact of cellulose nanofibrils (CNF) and
nanocrystals (CNC) is performed using materials provided by the Nanomaterial Health
Implications Research Consortium of the National Institute of Environmental Health
Sciences. An intermediary length of nanocrystals is also derived by acid
hydrolysis. While all CNFs and CNCs are devoid of cytotoxicity, 210 and 280 nm
fluorescein isothiocyanate (FITC)-labeled CNCs show higher cellular uptake than
longer and shorter CNCs or CNFs. Moreover, CNCs in the 200-300 nm length scale are
more likely to induce lysosomal damage, NLRP3 inflammasome activation, and IL-1
beta production than CNFs. The pro-inflammatory effects of CNCs are correlated with
higher crystallinity index, surface hydroxyl density, and reactive oxygen species
generation. In addition, CNFs and CNCs can induce maturation of bone marrow-derived
dendritic cells and CNCs (and to a lesser extent CNFs) are found to exert adjuvant
effects in ovalbumin (OVA)-injected mice, particularly for 210 and 280 nm CNCs. All
considered, the data demonstrate the importance of length scale, crystallinity, and
surface reactivity in shaping the innate immune response to nanocellulose.
AN - rayyan-553781570
AU - Wang, X.
AU - Chang, C. H.
AU - Jiang, J. H.
AU - Liu, Q.
AU - Liao, Y. P.
AU - Lu, J. Q.
AU - Li, L. J.
AU - Liu, X. S.
AU - Kim, J.
AU - Ahmed, A.
AU - Nel, A. E.
AU - Xia, T.
DO - 10.1002/smll.201901642
IS - 42
KW - Cellulose
PY - 2019
SN - 1613-6810 1613-6829
ST - The Crystallinity and Aspect Ratio of Cellulose Nanomaterials Determine Their
Pro-Inflammatory and Immune Adjuvant Effects In Vitro and In Vivo
T2 - SMALL
TI - The Crystallinity and Aspect Ratio of Cellulose Nanomaterials Determine Their
Pro-Inflammatory and Immune Adjuvant Effects In Vitro and In Vivo
VL - 15
Y2 - 10
ID - 9508
ER -
TY - JOUR
AB - Quantum dots (QDs) are nanoparticles with a particle size of 1–10 nm. Typical
QDs are made of compounds such as cadmium sulfide, cadmium selenide, silver
sulfide, and indium phosphide, among others. QDs exhibit promising potential for a
wide range of applications owing to their excellent optical properties. With the
rise in the application of and demand for QDs, QDs accumulation in the environment
has increased markedly. QDs enter the pulmonary system via inhalation and trigger
pulmonary toxicity. This paper first reviews the pulmonary toxicity of different
types of QDs in vivo and in vitro. Regarding acute toxicity, QDs cause changes in
cell morphology, cell membrane disruption, cell viability, and pulmonary
inflammation. Regarding chronic toxicity, cadmium-based QDs cause pulmonary
granulomas and have a potential carcinogenic risk. Second, this paper presents an
overview of the pulmonary toxicity mechanism of QDs, involving oxidative stress,
inflammation, autophagy, apoptosis, and ferroptosis. It summarizes mitogen-
activated protein kinases, nuclear factor κB, nuclear factor-erythroid 2-related
factor 2, P53, and Phosphoinositide 3-kinase/AKT signaling pathways in apoptosis
and autophagy. Third, it enumerates the physicochemical properties of QDs
influencing pulmonary toxicity, ranging from components, surface functional groups,
size, and surface charge. Lastly, it outlines the shortcomings of current studies
on QDs pulmonary toxicity. The paper concludes with a recommendation discussing
research-based improvements in the physicochemical properties of QDs to reduce
their release in the environment. © 2022 Elsevier B.V.
AN - rayyan-553781571
AU - Wang, X.
AU - He, K.
AU - Hu, Y.
AU - Tang, M.
DO - 10.1016/j.cbi.2022.110247
KW - Mechanism
Pulmonary
Quantum dots
Toxicity
Cell Survival
Nanoparticles
Particle Size
Phosphatidylinositol 3-Kinases
Quantum Dots
cadmium
CXCL1 chemokine
CXCL2 chemokine
doxorubicin
epithelial derived neutrophil activating factor 78
indium
interleukin 1beta
interleukin 33
interleukin 6
macrogol
nanoparticle
phosphatidylinositol 3 kinase
protein p53
quantum dot
selenium
silica nanoparticle
silver
silver sulfide
synaptophysin
unclassified drug
zinc oxide
acute toxicity
apoptosis
biology
cell membrane
cell structure
cell viability
chronic toxicity
environment
ferroptosis
human
inflammation
lung
lung toxicity
lymphocyte
macrophage
neutrophil
Nrf2 signaling
oxidative stress
particle size
photoluminescence
physical chemistry
physical phenomena
pneumonia
protein aggregation
review
Review
surface charge
cell survival
chemistry
PY - 2022
ST - A review of pulmonary toxicity of different types of quantum dots in
environmental and biological systems
TI - A review of pulmonary toxicity of different types of quantum dots in
environmental and biological systems
85140952305&doi=10.1016%2fj.cbi.2022.110247&partnerID=40&md5=8dbc0443a4cf41470970a2
aae642e098
VL - 368
ID - 9509
ER -
TY - JOUR
AB - Periodontitis is a chronic inflammatory disease initiated by pathogenic
biofilms and host immunity that damages tooth-supporting tissues, including the
gingiva, periodontal ligament and alveolar bone. The physiological functions of the
oral cavity, such as saliva secretion and chewing, greatly reduce the residence of
therapeutic drugs in the area of a periodontal lesion. In addition, complex and
diverse pathogenic mechanisms make effectively treating periodontitis difficult.
Therefore, designing advanced local drug delivery systems and rational therapeutic
strategies are the basis for successful periodontitis treatment. Hydrogels have
attracted considerable interest in the field of periodontitis treatment due to
their biocompatibility, biodegradability and convenient administration to the
periodontal pocket. In recent years, the focus of hydrogel research has shifted to
smart stimuli-responsive hydrogels, which can undergo flexible sol-gel transitions
in situ and control drug release in response to stimulation by temperature, light,
pH, ROS, glucose, or enzymes. In this review, we systematically introduce the
development and rational design of emerging smart stimuli-responsive hydrogels for
periodontitis treatment. We also discuss the state-of-the-art therapeutic
strategies of smart hydrogels based on the patho-genesis of periodontitis.
Additionally, the challenges and future research directions of smart hydrogels for
periodontitis treatment are discussed from the perspective of developing efficient
hydrogel delivery systems and potential clinical applications.
AN - rayyan-553781576
AU - Wang, Y. X.
AU - Li, J. X.
AU - Tang, M. M.
AU - Peng, C. J.
AU - Wang, G. C.
AU - Wang, J. J.
AU - Wang, X. R.
AU - Chang, X. W.
AU - Guo, J.
AU - Gui, S. Y.
DO - 10.1016/j.biopha.2023.114688
KW - Periodontics
PY - 2023
SN - 0753-3322 1950-6007
ST - Smart stimuli-responsive hydrogels for drug delivery in periodontitis
treatment
TI - Smart stimuli-responsive hydrogels for drug delivery in periodontitis
treatment
VL - 162
Y2 - 6
ID - 9514
ER -
TY - JOUR
AB - Angioplasty with stents is the most important method for the treatment of
coronary artery disease (CAD). However, the drug-eluting stents (DES) that are
widely used have the increased risks of inflammatory reactions and late stent
thrombosis (LST) because of the persistence of the polymer coatings. To improve the
biosafety, a novel polymer-free-composite drug-eluting coating composed of magnetic
mesoporous silica nanoparticles (MMSNs) and carbon nanotubes (CNTs) was constructed
using the electrophoretic deposition (EPD) method in this study. A crack-free two-
layered coating with impressive network nanotopologies was successfully obtained by
regulating the composition and structures. This nanostructured coating exhibits
excellent mechanical flexibility and blood compatibility in vitro, and the drug-
loading and release performance is satisfactory as well. The in vivo study shows
that this composite coating has the obvious advantage of rapid endothelialization
because of its unique 3D nanostructured topology in comparison with the commercial
polymer-coated DES. This study aims to provide new ideas and reliable data to
design novel functional coatings that could accelerate the re-endothelialization
process and avoid inflammatory reactions, thus improving the in vivo biosafety of
DES.
AN - rayyan-553782304
AU - Wang, Y.
AU - Zhang, W.
AU - Zhang, J.
AU - Sun, W.
AU - Zhang, R.
AU - Gu, H.
DO - 10.1021/am403365j
IS - 20
KW - Animals
Antibiotics, Antineoplastic/chemistry/pharmacokinetics/therapeutic use
Blood Cells/drug effects
Coronary Artery Disease/drug therapy
*Drug-Eluting Stents
Half-Life
Hemolysis/drug effects
Magnetics
Male
Metal Nanoparticles/chemistry
Nanostructures/*chemistry/toxicity
Nanotubes, Carbon/chemistry
Polymers/chemistry
Porosity
Rabbits
Silver/chemistry
Sirolimus/chemistry/pharmacokinetics/therapeutic use
Stents
Polymerization
Polymers
LA - eng
N1 - Nano Biomedical Research center, School of Biomedical Engineering and Med-X
Research Institute, Shanghai Jiao Tong University , Shanghai 200030, China.
PY - 2013
SP - 10337-45
ST - Fabrication of a novel polymer-free nanostructured drug-eluting coating for
cardiovascular stents
TI - Fabrication of a novel polymer-free nanostructured drug-eluting coating for
cardiovascular stents
VL - 5
Y2 - 10 y3 - 23
ID - 10212
ER -
TY - JOUR
AB - Aims: To study the histological spectrum of lymphadenopathy in human
immunodeficiency virus (HIV) infected Thai patients. Methods: Lymph nodes from 55
HIV infected patients were accessioned over a 19 month period in two pathology
laboratories in Bangkok, Thailand. These were examined with H&E, Ziehl-Neelsen,
periodic acid-Schiff (PAS), PAS with diastase (PAS/D), Gram and methenamine stains.
Results: Six reaction patterns were observed: (1) classic necrotising granulomas
(30 cases); (2) extensive necrosis with minimal granulomatous response (5 cases);
(3) sarcoid-like non-necrotising granulomas (5 cases); (4) foamy macrophage or
pseudo-Gaucher cell response (5 cases); (5) inflammatory pseudotumour-like
proliferation (3 cases); and (6) non-specific lymphoid hyperplasia (7 cases).
Myriads of intracellular, long, slender acid-fast bacilli were found in those cases
with the pseudo-Gaucher cell and inflammatory pseudotumour-like response, while
variable numbers of bacilli were identified in those cases with non-necrotising
sarcoid-like granulomas. Few scattered acid-fast bacilli were found in five cases
with necrotising granulomas. In one case, yeast-like organisms in keeping with
Cryptococcus were identified. No organisms were identified in the cases showing
lymphoid hyperplasia, extensive necrosis and minimal granulomatous response, and in
the remaining cases of classic necrotising granulomas. Conclusions: The wide
spectrum of histological changes in HIV-associated lymphadenomegaly requires
recognition, particularly as the majority were associated with acid-fast organisms,
mostly in keeping with the morphological features of Mycobacterium avium-M.
intracellulare complex that was distinctively stained by Grocott methenamine-
silver, Gram and PAS stains. The histological changes mimic those of infarction and
other infective lymphadenitis, sarcoidosis, Whipple's disease, inflammatory
pseudotumour and spindle cell neoplasms. © 2007 Royal College of Pathologists of
Australasia.
AN - rayyan-553781582
AU - Wannakrairot, P.
AU - Leong, T. Y. M.
AU - Leong, A. S. Y.
DO - 10.1080/00313020701230674
IS - 2
KW - Apoptosis
Caseous necrosis
Granulomatous inflammation
Histoid reaction
HIV
Inflammatory pseudotumour
Lymph node
Mycobacteria
Pseudo-Gaucher cells
AIDS-Related Opportunistic Infections
HIV Infections
Humans
Lymphatic Diseases
Mycobacterium avium Complex
Mycobacterium avium-intracellulare Infection
Thailand
acid fast bacterium
article
bacterium detection
cell type
clinical feature
Cryptococcus
Gram staining
granuloma
histopathology
human
Human immunodeficiency virus infected patient
human tissue
intestine lipodystrophy
lymph node hyperplasia
major clinical study
mucocutaneous lymph node syndrome
Mycobacterium intracellulare avium
pseudotumor
sarcoidosis
spindle cell carcinoma
tissue section
PY - 2007
SP - 223-227
ST - The morphological spectrum of lymphadenopathy in HIV infected patients
T2 - Pathology
TI - The morphological spectrum of lymphadenopathy in HIV infected patients
33947328869&doi=10.1080%2f00313020701230674&partnerID=40&md5=d506dd8339e7f5d9def694
56a0141203
VL - 39
ID - 9520
ER -
TY - JOUR
AB - The problem of multidrug resistance in bacterial pathogens is significant and
is related to the high morbidity and death rates of living things due to increased
levels of beta-lactamases. Plant-derived nanoparticles have gained a great
significance in the field of science and technology to combat bacterial diseases,
especially multidrug-resistant bacteria. This study examines the multidrug
resistance and virulent genes of identified pathogenic Staphylococcus species
obtained from Molecular Biotechnology and Bioinformatics Laboratory (MBBL), culture
collection. The polymerase chain reaction-based characterization of Staphylococcus
aureus and Staphylococcus argenteus having ON875315.1 and ON876003.1 accession IDs
revealed the presence of the spa, LukD, fmhA, and hld genes. The green synthesis of
silver nanoparticles (AgNPs) was carried out by utilizing the leaf extract of
Calliandra harrisii, of which metabolites act as capping and reducing agents for
the precursor of nano-synthesis, i.e., AgNO3 of 0.25 M. The synthesized AgNPs were
characterized via UV-vis spectroscopy, Fourier transform infrared spectroscopy,
scanning electron microscopy, and energy-dispersive X-ray analysis which inferred
the bead-like shape of our nanoparticles with the size of 2.21 nm with the
existence of aromatic and hydroxyl functional groups at surface plasmon resonance
of 477 nm. The antimicrobial activity by AgNPs showed 20 mm inhibition of
Staphylococcus species as compared to the vancomycin and cefoxitin antibiotics
along with crude plant extract, which showed a minimum zone of inhibition. The
synthesized AgNPs were also analyzed for various biological activities like anti-
inflammatory with 99.15% inhibition in protein denaturation, antioxidant with 99.8%
inhibition in free radical scavenging, antidiabetic with 90.56% inhibition of alpha
amylase assay, and anti-haemolytic with 89.9% inhibition in cell lysis which shows
good bioavailability and biocompatibility of the nanoparticles with the biological
system of the living being. The amplified genes (spa, LukD, fmhA, and hld) were
also analyzed for their interaction with AgNPs computationally at the molecular
level. The 3-D structure of AgNP and amplified genes was retrieved from ChemSpider
(ID: 22394) and Phyre2 online server, respectively. The binding affinities of AgNP
with spa, LukD, fmhA, and hld were −7.16, −6.5, −6.45, and −3.3 kJ/mol,
respectively, which infers a good docking score except of hld which is −3.3 kJ/mol
due to its small size. The salient features of biosynthesized AgNPs proved to be an
effective approach in combating the multidrug-resistant Staphylococcus species in
the future. © 2023 The Authors. Published by American Chemical Society.
AN - rayyan-553781583
AU - Waseem, M.
AU - Naveed, M.
AU - Rehman, S. U.
AU - Makhdoom, S. I.
AU - Aziz, T.
AU - Alharbi, M.
AU - Alsahammari, A.
AU - Alasmari, A. F.
DO - 10.1021/acsomega.3c01597
IS - 23
KW - Staphylococcus
Computer Simulation
PY - 2023
SP - 20920-20936
ST - Molecular Characterization of spa, hld, fmhA, and lukD Genes and
Computational Modeling the Multidrug Resistance of Staphylococcus Species through
Callindra harrisii Silver Nanoparticles
T2 - ACS Omega
TI - Molecular Characterization of spa, hld, fmhA, and lukD Genes and
Computational Modeling the Multidrug Resistance of Staphylococcus Species through
Callindra harrisii Silver Nanoparticles
85163361322&doi=10.1021%2facsomega.3c01597&partnerID=40&md5=3f37d13aeedeef75bbb101d
e571a90e2
VL - 8
ID - 9521
ER -
TY - JOUR
AB - Scanning and transmission electron microscopy are of clinical value in
assessing the interaction between biomaterials and ingrowing tissues.
Ultrastructural information allows the clinician and biomaterials specialist to
determine events occurring during wound healing and the biocompatibility of
prosthetic devices. This paper reviews some of the experimental and clinical
studies done in our laboratory on the use of natural and reconstituted collagen as
replacements for connective tissues. Consideration is given to collagen flakes used
for the treatment of dermal ulcers, a collagen fiber prosthesis used for tendon and
ligament replacement, the effects of chemical preservatives on cartilage used for
replacement of tissues during plastic surgery and the growth and orientation of
nerve cells on reconstituted collagen fibers. Our results show that reconstituted
collagen can be prepared into prosthetic devices which encourage cell attachment
and orientation thereby facilitating healing of injured tissues. Furthermore
chemical preservation of cartilagenous tissues kills chondrocytes resulting in
eventual resorption by inflammatory cells.
AN - rayyan-553782142
AU - Wasserman, A. J.
AU - Doillon, C. J.
AU - Glasgold, A. I.
AU - Kato, Y. P.
AU - Christiansen, D.
AU - Rizvi, A.
AU - Wong, E.
AU - Goldstein, J.
AU - Silver, F. H.
IS - 3
J2 - Scanning Microsc
Collagen/*ultrastructure
Connective Tissue/*ultrastructure
Humans
Microscopy, Electron
Collagen
LA - eng
N1 - Biomaterials Center, UMDNJ-Robert Wood Johnson Medical School, Piscataway
08854.
PY - 1988
SN - 0891-7035 (Print)
SP - 1635-46
ST - Clinical applications of electron microscopy in the analysis of collagenous
biomaterials
T2 - Scanning microscopy
TI - Clinical applications of electron microscopy in the analysis of collagenous
biomaterials
VL - 2
Y2 - 9
ID - 10053
ER -
TY - COMP
AB - OBJECTIVES: This study was conducted to 1) measure the sensitivity of human
and mouse macrophages to metal ions which are released from dental biomaterials, 2)
compare these sensitivities with those of other cell types in the oral cavity, and
3) determine if metal ions alter the metabolism and synthetic processes of these
cells at lower concentrations than are required to lyse the cells. This information
will help define the biological risks associated with the release of metal ions
into the oral cavity. METHODS: Macrophages were exposed to a range of
concentrations of Ag1+, Au3+, Cu2+, Hg2+, Ni2+, Pd2+, Pt4+, and Zn2+ for 24 h in
cell culture. The concentrations which caused a 50% decrease in succinic
dehydrogenase (SDH) activity, protein production, and lactate dehydrogenase (LDH)
release were measured and compared with these values for fibroblasts and
osteoblasts. RESULTS: Most metal ions caused alteration in SDH activity and protein
production at lower concentrations than were required to induce LDH release. There
were exceptions to this trend, and the differences were not always statistically
significant. Furthermore, although the macrophages sometimes had statistically
different sensitivities to metal ions than fibroblasts or osteoblasts, these
differences were less than one order of magnitude. Macrophage response to the metal
ions was highly dependent on the metal ion and the species of macrophage.
SIGNIFICANCE: Macrophages react adversely to metal ions at similar concentrations
as other cell types found in the oral cavity. Furthermore, the concentrations which
affect cell metabolism and protein production are generally lower than those which
lyse the cells. Thus, non-lethal concentrations of metal ions may alter the
secretion of protein inflammatory mediators such as cytokines which direct the
inflammatory response in tissues.
AN - rayyan-553782147
AU - Wataha, J. C.
AU - Hanks, C. T.
AU - Sun, Z.
CY - England
DO - 10.1016/0109-5641(95)80056-5
ET - 4
J2 - Dent Mater
KW - Analysis of Variance
Animals
Cells, Cultured
Copper/toxicity
Dental Materials/*toxicity
Fibroblasts/drug effects/metabolism
Gold/toxicity
Humans
Ions
L-Lactate Dehydrogenase/metabolism
Macrophages/*drug effects/metabolism
Mercury/toxicity
Metals/*toxicity
Mice
Nickel/toxicity
Osteoblasts/drug effects/metabolism
Palladium/toxicity
Platinum/toxicity
Protein Biosynthesis
Silver/toxicity
Succinate Dehydrogenase/metabolism
Zinc/toxicity
Macrophages
Metals
LA - eng
N1 - Department of Oral Rehabilitation, Medical College of Georgia School of
Dentistry, Augusta, USA.
PY - 1995
SN - 0109-5641 (Print)
SP - 239-45
ST - In vitro reaction of macrophages to metal ions from dental biomaterials
T2 - Dental materials : official publication of the Academy of Dental Materials
TI - In vitro reaction of macrophages to metal ions from dental biomaterials
VL - 11
Y2 - 7
ID - 10058
ER -
TY - JOUR
AB - Green synthesis of silver nanoparticles (AgNPs) has been extensively studied
by using a variety of plant extracts for applications in biomedical sciences and
engineering. However, there are no reports on the synthesis of AgNPs by utilizing
the berry extract of Sea Buckthorn, which is a traditional Chinese medicine and
exhibits a wide spectrum of antioxidant, anti-inflammatory and anticancer
activities. In this paper, we report an easy and eco-friendly technique for the
preparation of AgNPs using the Sea Buckthorn berry extract under ultrasonic
radiation at ambient temperature and the evaluation of both biosynthesis parameters
and biological activities. The UV-visible spectrum and dynamic light scattering
(DLS) analysis showed that the size of AgNPs was sensitive to the biosynthesis
parameters, such as the pH of the extract, material proportion and reaction time,
offering a size-controlled synthetic method for AgNPs. The X-ray diffraction (XRD),
transmission electron microscopy (TEM), selected area electron diffraction (SAED)
and DLS studies showed that the AgNPs (pH 10.0; material proportion 1 : 1; 4 h) had
a face-centered cubic (fcc) structure and spherical shape with an average particle
size of 27.3 +/- 0.2 nm covered by anions, and existed in a monodispersed form with
a polydispersity index (PDI) of 0.213. The biosynthesized AgNPs showed potent
anticancer activity against human colorectal cancer (HCT116 and SW620), hepatoma
cancer (HepG2), breast cancer (MCF-7) and cervical cancer (HeLa) cell lines as well
as strong antioxidant activity. The IC50 values for these five cell lines were
8.77, 4.61, 14.59, 16.05 and 27.98 mu g mL(-1), respectively. However, the
biosynthesized AgNPs revealed poor inhibition activities for the growth of E. coli
and S. aureus. These results confirmed that the Sea Buckthorn could be a low-cost,
nontoxic and eco-friendly natural resource for the synthesis of AgNPs, which might
be useful for the development of new alternative antioxidant and anticancer agents
in biomedicine.
AN - rayyan-553781586
AU - Wei, S. M.
AU - Wang, Y. H.
AU - Tang, Z. S.
AU - Hu, J. H.
AU - Su, R.
AU - Lin, J. J.
AU - Zhou, T.
AU - Guo, H.
AU - Wang, N.
AU - Xu, R. R.
DO - 10.1039/d0nj01335h
IS - 22
KW - Fruit
PY - 2020
SN - 1144-0546 1369-9261
SP - 9304-9312
ST - A size-controlled green synthesis of silver nanoparticles by using the berry
extract of Sea Buckthorn and their biological activities
TI - A size-controlled green synthesis of silver nanoparticles by using the berry
extract of Sea Buckthorn and their biological activities
VL - 44
Y2 - 6 y3 - 14
ID - 9524
ER -
TY - JOUR
AB - Biomaterial-induced infection and inadequate osteointegration are regarded as
two major reasons for the failure of implants. Herein, niobium
(Nb)/polyetherketoneketone (PEKK) composite (NPC) was prepared, and a gallium (Ga)-
Nb nanofiber surface on NPC (NPCG) was fabricated through sequential treatment with
sodium hydroxide and gallium nitrate solution. Compared with PEKK and NPC, NPCG
with nanofiber surface exhibited significant enhancements in surface properties
with higher hydrophilicity and roughness, etc. NPCG remarkably facilitated
multiplication and osteoblastic differentiation of bone mesenchymal stem cells in
vitro. Moreover, NPCG obviously promoted the RAW264.7 cells polarization to M2
macrophages, which secreted anti-inflammatory cytokines in vitro, demonstrating an
anti-inflammatory effect. Furthermore, NPCG significantly boosted osteogenesis and
ameliorated osseointegration in vivo. The enhancements of osteoblastic response and
M2 macrophage polarization in vitro and osseointegration in vivo for NPCG were
attributed to the Ga-Nb nanofiber surface. Further, NPCG displayed outstanding
bactericidal capability, which not only inhibited the bacteria growth in vitro but
also resisted infection in vivo owing to the sustained release of Ga ions from the
nanofiber surface. In short, NPCG with good biocompatibility exhibited anti-
inflammatory, bactericidal and osteogenic capabilities, which facilitated
osteoblastic differentiation and M2 macrophage polarization, and ameliorated
osseointegration, thereby revealing great potential for bone substitute in
orthopedics.
AN - rayyan-553781587
AU - Wei, W.
AU - Yang, R. X.
AU - Yu, Q.
AU - Zhao, J.
AU - Li, W. Z.
DO - 10.1016/j.compositesb.2022.110375
KW - Osteoblasts
Osteogenesis
PY - 2023
SN - 1359-8368 1879-1069
ST - Gallium-niobium nanofiber surface of niobium/PEKK composite with anti-
inflammatory, osteogenesis and anti-bacterial effects for facilitating osteoblastic
differentiation and ameliorating osteointegration
T2 - COMPOSITES PART B-ENGINEERING
TI - Gallium-niobium nanofiber surface of niobium/PEKK composite with anti-
inflammatory, osteogenesis and anti-bacterial effects for facilitating osteoblastic
differentiation and ameliorating osteointegration
VL - 248
Y2 - 1 y3 - 1
ID - 9525
ER -
TY - JOUR
AB - In traditional blood-contacting medical devices, infection and thrombosis are
easily formed on the surface of the materials. In addition, inflammation is also a
clinical complication that cannot be ignored. More importantly, there is a mutually
promoting relationship between the inflammatory response and the infection as well
as thrombosis. In this work, we propose a self-adaptive anti-inflammatory coating
strategy combined with anti-infection and anticoagulant capacity, which was
accomplished based on nano-Ag particles and dexamethasone (Dex)-loaded hydrogel
coating. The coating loaded with nano-Ag endows it with good bactericidal
performance, including Gram-positive and Gram-negative bacteria. As an anti-
inflammatory drug, Dex was grafted onto hydrogel coating by a reactive oxygen
species (ROS)-cleavable thioketal (TK) bond and released upon the trigger of an
inflammatory environment, blocking further inflammatory cascade, providing self-
adaptive anti-inflammatory properties, and avoiding side effects of the drug. It
was demonstrated that the coating worked as a precise strategy to resist
coagulation, infection, and inflammation, provided a new perspective for designing
clinical complication-conformable coatings, and had great application prospects on
blood-contacting medical devices. © 2022 American Chemical Society. All rights
reserved.
AN - rayyan-553781588
AU - Wei, Y.
AU - Liu, J.
AU - Liu, G.
AU - Gao, S.
AU - Wu, D.
AU - Yang, L.
AU - Luo, R.
AU - Zhang, F.
AU - Wang, Y.
DO - 10.1021/acs.biomac.2c00815
IS - 10
KW - Anti-Bacterial Agents
Anticoagulants
Dexamethasone
Gram-Negative Bacteria
Gram-Positive Bacteria
Humans
Hydrogels
Inflammation
Surface Properties
Thrombosis
Bacteria
Biofilms
Blood
Diseases
Pathology
dexamethasone
hydrogel
interleukin 1beta
silver nanoparticle
anticoagulant agent
antiinfective agent
biocompatible coated material
Anti-infection
Anti-inflammatories
Clinical complications
Contacting devices
Dexamethasones
Haemocompatibility
Hydrogel coatings
Medical Devices
Property
aqueous solution
Article
bacterial viability
biocompatibility
blood compatibility
body weight gain
circulation
coating thickness
contact angle
controlled study
drug coating
erythrocyte adhesiveness
Escherichia coli
human
in vitro study
inflammation
macrophage
New Zealand White (rabbit)
nonhuman
polymerization
RAW 264.7 cell line
chemistry
pharmacology
surface property
thrombosis
Coatings
PY - 2022
SP - 4357-4369
ST - Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered
Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device
T2 - Biomacromolecules
TI - Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered
Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device
85139265106&doi=10.1021%2facs.biomac.2c00815&partnerID=40&md5=abd01d358ef1aaeb10d7d
f3e8dcaf99d
VL - 23
ID - 9526
ER -
TY - JOUR
AB - Acellular matrices are mainly composed of mammalian tissues, and aquatic
tissues with lower biological risks and less religious restrictions are considered
alternatives to mammalian tissues. The acellular fish skin matrix (AFSM) has been
commercially available. Silver carp has the advantages of farmability, high yield
and low price, but there are few studies on the silver carp acellular fish skin
matrix (SC-AFSM). In this study, an acellular matrix with low DNA and endotoxin was
prepared from the skin of silver carp. After treatment with trypsin/sodium dodecyl
sulfate and Triton X-100 solutions, the DNA content in SC-AFSM reached 11.03 ± 0.85
ng/mg, and the endotoxin removal rate was 96.8%. The porosity of SC-AFSM was 79.64%
± 0.17%, which is favorable for cell infiltration and proliferation. The relative
cell proliferation rate of SC-AFSM extract was 117.79% ± 15.26%. The wound healing
experiment showed that SC-AFSM had no adverse acute pro-inflammatory response,
which had a similar effect as commercial products in promoting tissue repair.
Therefore, SC-AFSM has great application potential in biomaterials. © 2023 Wiley
Periodicals LLC.
AN - rayyan-553781589
AU - Wei, Z.
AU - Zhang, J.
AU - Guo, Z.
AU - Wu, Z.
AU - Sun, Y.
AU - Wang, K.
AU - Duan, R.
DO - 10.1002/jbm.b.35236
IS - 6
KW - acellular matrix
biomaterials
DNA content
endotoxin
silver carp skin
Animals
Carps
Endotoxins
Mammals
Cell proliferation
DNA
Histology
Silver
Sulfur compounds
Tissue
triton x 100
trypsin
Acellular matrices
Biological risks
Endotoxin
Higher yield
Mammalian tissues
Property
Silver carp
Silver carp skin
animal experiment
animal tissue
Article
biocompatibility
cell infiltration
cell proliferation
controlled study
cytotoxicity test
decellularization
Hypophthalmichthys molitrix
male
mouse
nonhuman
porosity
tissue repair
wound healing
wound healing assay
animal
carp
mammal
Fish
Skin
PY - 2023
SP - 1328-1335
ST - Study on the preparation and properties of acellular matrix from the skin of
silver carp (Hypophthalmichthys molitrix)
T2 - Journal of Biomedical Materials Research - Part B Applied Biomaterials
TI - Study on the preparation and properties of acellular matrix from the skin of
silver carp (Hypophthalmichthys molitrix)
85148608241&doi=10.1002%2fjbm.b.35236&partnerID=40&md5=7c37332862ba9323580738ec4a68
154d
VL - 111
ID - 9527
ER -
TY - JOUR
AB - BACKGROUND: One of the major limitations of biliary stents is the stent
occlusion, which is closely related to the over-growth of bacteria. This study
aimed to evaluate the feasibility of a novel silver-nanoparticle-coated
polyurethane (Ag/PU) stent in bacterial cholangitis model in swine. METHODS: Ag/PU
was designed by coating silver nanoparticles on polyurethane (PU) stent. Twenty-
four healthy pigs with bacterial cholangitis using Ag/PU and PU stents were
randomly divided into an Ag/PU stent group (n=12) and a PU stent group (n=12),
respectively. The stents were inserted by standard endoscopic retrograde
cholangiopancreatography. Laboratory assay was performed for white blood cell (WBC)
count, alanine aminotransferase (ALT), interleukin-1beta (IL-1beta), tumor necrosis
factor-alpha (TNF-alpha) at baseline time, 8 hours, 1, 2, 3, and 7 days after stent
placements. The segment of bile duct containing the stent was examined
histologically ex vivo. Implanted biliary stents were examined by a scan electron
microscope. The amount of silver release was also measured in vitro. RESULTS: The
number of inflammatory cells and level of ALT, IL-1beta and TNF-alpha were
significantly lower in the Ag/PU stent group than in the PU stent group.
Hyperplasia of the mucosa was more severe in the PU stent group than in the Ag/PU
stent group. In contrast to the biofilm of bacteria on the PU stent, fewer bacteria
adhered to the Ag/PU stent. CONCLUSIONS: PU biliary stents modified with silver
nanoparticles are able to alleviate the inflammation of pigs with bacterial
cholangitis. Silver-nanoparticle-coated stents are resistant to bacterial adhesion.
AN - rayyan-553782101
AU - Wen, W.
AU - Ma, L. M.
AU - He, W.
AU - Tang, X. W.
AU - Zhang, Y.
AU - Wang, X.
AU - Liu, L.
AU - Fan, Z. N.
DO - 10.1016/s1499-3872(15)60410-6
IS - 1
J2 - Hepatobiliary Pancreat Dis Int
KW - Alanine Transaminase/blood
Animals
Anti-Bacterial Agents/*administration & dosage
Bacterial Adhesion/*drug effects
Biofilms/*drug effects/growth & development
Biomarkers/blood
Cholangiopancreatography, Endoscopic Retrograde/*instrumentation
Cholangitis/blood/diagnosis/microbiology/*therapy
*Coated Materials, Biocompatible
Cytokines/blood
Feasibility Studies
Inflammation Mediators/blood
*Nanoparticles
Polyurethanes
Prosthesis Design
Prosthesis Failure
Silver/*administration & dosage
Stents/*microbiology
Swine
Time Factors
Stents
Cholangitis
LA - eng
N1 - Department of Digestive Endoscopy Center, First Affiliated Hospital of
Nanjing Medical University, Nanjing 210029, China. fanzhining@njmu.edu.cn.
PY - 2016
SN - 1499-3872 (Print)
SP - 87-92
ST - Silver-nanoparticle-coated biliary stent inhibits bacterial adhesion in
bacterial cholangitis in swine
T2 - Hepatobiliary & pancreatic diseases international : HBPD INT
TI - Silver-nanoparticle-coated biliary stent inhibits bacterial adhesion in
bacterial cholangitis in swine
VL - 15
Y2 - 2
ID - 10012
ER -
TY - JOUR
AB - Suppression of angiotensin II formation by angiotensin-converting enzyme
inhibitors or blockade of the angiotensin II receptor by angiotensin receptor
blockers is a powerful therapeutic strategy to slow the progression of renal
disease. However, angiotensin-converting enzyme inhibitors and angiotensin receptor
blockers provide only imperfect protection against the progression of chronic
kidney disease to end-stage renal failure. Hence, innovative approaches are needed
to keep patients with chronic kidney disease off dialysis. Angiotensin II activates
at least two receptors, namely the angiotensin II type 1 (AT1) and angiotensin II
type 2 (AT2) receptors. The majority of the effects of angiotensin II, such as
vasoconstriction, inflammation, and matrix deposition, are mediated via the AT1
receptor. It is thought that the AT2 receptor counteracts these effects and plays a
role in nephroprotection. However, recent data support the notion that the AT2
receptor transduces pro-inflammatory effects and promotes fibrosis and hypertrophy.
Therefore, the question of whether stimulation of the AT2 receptor could represent
a silver bullet for the treatment of chronic kidney disease or may, on the
contrary, exert detrimental effects on renal physiology remains unresolved. Recent
data from AT2 receptor-knockout mice demonstrate that the loss of AT 2 receptor
signalling is associated with increased renal injury and mortality in chronic
kidney disease. This raises the expectation that pharmacological stimulation of the
AT2 receptor may positively influence renal pathologies. However, further research
is needed to explore the question whether AT2 receptor stimulation may represent a
new therapeutic strategy for the treatment of chronic kidney disease.
AN - rayyan-553781592
AU - Wenzel, U. O.
AU - Krebs, C.
AU - Benndorf, R.
DO - 10.1177/1470320309347787
IS - 1
KW - Angiotensin
AT2 receptor
Fibrosis
Inflammation
Kidney
Angiotensin II Type 1 Receptor Blockers
Animals
Humans
Kidney Diseases
Kidney Failure, Chronic
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
angiotensin 1 receptor
angiotensin 2 receptor
angiotensin receptor antagonist
aldosterone release
apoptosis
blood pressure regulation
cell growth
chronic kidney disease
disease course
fibrosis
hormone action
human
hypertrophy
kidney development
knockout mouse
negative feedback
nonhuman
pathogenesis
pathophysiology
pharmacodynamics
renal protection
review
sodium transport
vasoconstriction
PY - 2010
SP - 37-41
ST - The angiotensin II type 2 receptor in renal disease
T2 - JRAAS - Journal of the Renin-Angiotensin-Aldosterone System
TI - The angiotensin II type 2 receptor in renal disease
77749254986&doi=10.1177%2f1470320309347787&partnerID=40&md5=68a6bc0dc46fc49c4348449
2c58f99eb
VL - 11
ID - 9530
ER -
TY - JOUR
AB - Nanocrystalline silver (NCS) has proven to be an important wound dressing
particularly in chronic infected wounds. However, debate still rages around its use
in the case of partially epithelialized wounds, particularly when these are non-
infected. Much of the debate has revolved around seemingly contradictory research
publications that blurred the use of NCS in these clinical situations, primarily
based on reported cytotoxic effects of NCS on cell lines in vitro. MMPs, in
particular MMP-9 (gelatinase) has been demonstrated to be pivotal in the
progression from keratinocyte cleavage, to migration and re-epithelialisation. High
levels promote increases in TNF-α; IL-8 and TGFβ, all associated with exaggerated
ongoing inflammation and chronicity. Low levels impede the process of keratinocyte
migration. Thus, as in so many clinical situations, a balance of MMP level is
extremely important. NCS has been demonstrated to decrease these undesirable high
levels of MMP-9 making it an ideal dressing for chronic infected wounds, acute
inflamed wounds and burn wounds of all types which are associated with protracted
raised MMP-9 levels. The converse applies too - NCS used in a situation of minimal
inflammation may undesirably decrease the low levels of MMP-9 and adversely affect
epithelialisation. NCS would be contra-indicated in conjunction with cell lines in
vitro, cell cultured lines in vivo and integrated artificial matrices with added
cell lines. Therapeutic decisions for different clinical situations may thus be
made more predictably. © 2010 Elsevier Ltd and ISBI. All rights reserved.
AN - rayyan-553781593
AU - Widgerow, A. D.
DO - 10.1016/j.burns.2010.01.010
IS - 7
KW - Burns
Cytotoxicity
Gelatinases
MMP-9
Animals
Anti-Infective Agents, Local
Bandages
Cytotoxins
Honey
Humans
Nanoparticles
Silver
Skin
Swine
Wound Healing
Wound Infection
Wounds and Injuries
gelatinase A
gelatinase B
interleukin 8
nanocrystalline silver
petrolatum
silver
silver nitrate
steroid
tacrolimus
unclassified drug
apoptosis
burn
cell culture
cell line
cell migration
cell proliferation
cell viability
cytotoxicity
edema
epithelization
erythema
explant
fibroblast
honey
human
inflammation
keratinocyte
monolayer culture
nonhuman
review
skin allergy
wound care
wound dressing
wound healing
wound infection
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
PY - 2010
SP - 965-974
ST - Nanocrystalline silver, gelatinases and the clinical implications
T2 - Burns
TI - Nanocrystalline silver, gelatinases and the clinical implications
77957589985&doi=10.1016%2fj.burns.2010.01.010&partnerID=40&md5=7a3f886d874a9578d2ef
82287541dd9b
VL - 36
ID - 9531
ER -
TY - CPAPER
AB - Chronic wounds, such as venous, pressure, and diabetic ulcers, are difficult
to heal and represent a rising social and economical problem. Compared to acute
wounds, non-healing wounds contain elevated levels of neutrophil elastase, pro-
inflammatory cytokines (IL-1 beta, IL-6, IL-8), and matrix metalloproteases (MMP-2,
MMP-9, MMP-13) as well as free radicals. Their overproduction perpetuates the
inflammatory phase resulting in severe tissue damage and degradation of growth
factors. Consequently, wound closure is prevented and the wound remains non-healing
for month or even years. The increasing numbers of patients suffering from wounds
that fail to heal are a significant challenge for health care professionals. Wound
dressings play an important role in the entire management of these wounds. New
materials and treatment strategies are needed to improve wound care. Recent
advances in the field of biomaterials and their medical applications indicate the
significance and potential of various natural polymers in the development of novel
classes of wound dressings. Native polymers are an ideal source for bio-active
wound dressings because of their availability and biocompatibility. Hence, several
studies have been conducted to explore the influence of wound dressings consisting
of collagen, oxidized regenerated cellulose, bacterial cellulose, chitosan, or
alginate on the destructive milieu in chronic wounds.
AN - rayyan-553781594
AU - Wiegand, C.
AU - Hipler, U. C.
DO - 10.1002/masy.200900028
Polymerization
Polymers
Bandages
PY - 2010
SP - 1-13
T2 - UTILIZATION OF LIGNOCELLULOSIC MATERIALS
TI - Polymer-based Biomaterials as Dressings for Chronic Stagnating Wounds
VL - 294
ID - 9532
ER -
TY - JOUR
AB - Effective treatments to improve survivability following exposure to the nerve
agent soman have been established and are currently available. Unfortunately,
electrographic brain seizures, neuroinflammation and brain cell death are still a
potential problem even with treatment. In the present study we have characterized
the time course of the central neuro-inflammatory gene response using quantitative
real time-PCR (TaqMan). Male Sprague-Dawley rats were pre-treated with HI-6 (1-2-
hydroxy-iminomethyl-1-pyridino-3-(4-carbamoyl-1-pyridino-2-oxapropane dichloride);
125 mg/kg, i.p.) and exposed 30 min later to 1.6 x LD(50) of soman (pinacolyl
methyl-phosphonofluoridate, 180 microg/kg, s.c.) followed at 1 min by atropine
methyl nitrate (4 mg/kg, i.m.). Initially, a significant and dramatic upregulation
of tumor necrosis factor-alpha and vascular cell adhesion molecule-1 mRNA levels
was measured 2 h post-exposure followed at 6 h by upregulation of interleukin-
1beta, interleukin-6, E-selectin, and intercellular adhesion molecule-1 with
eventual resolution by 24-48 h. In conclusion, an acute and transient upregulation
of the inflammatory gene response is activated following soman exposure that may be
involved in the soman-induced brain injury process.
AN - rayyan-553782381
AU - Williams, A. J.
AU - Berti, R.
AU - Yao, C.
AU - Price, R. A.
AU - Velarde, L. C.
AU - Koplovitz, I.
AU - Schultz, S. M.
AU - Tortella, F. C.
AU - Dave, J. R.
DO - 10.1016/s0304-3940(03)00818-8
IS - 3
J2 - Neurosci Lett
KW - Animals
Atropine/pharmacology
Brain/*drug effects/metabolism/physiopathology
Chemical Warfare Agents/*toxicity
E-Selectin/genetics
Encephalitis/*chemically induced/*genetics/metabolism
Gene Expression Regulation/drug effects/genetics
Inflammation Mediators/*metabolism
Intercellular Adhesion Molecule-1/genetics
Male
Muscarinic Antagonists/pharmacology
Neurons/*drug effects/metabolism/pathology
RNA, Messenger/drug effects/metabolism
Rats
Reaction Time/drug effects/physiology
Soman/*toxicity
Tumor Necrosis Factor-alpha/genetics
Up-Regulation/drug effects/genetics
Vascular Cell Adhesion Molecule-1/genetics
LA - eng
N1 - Walter Reed Army Institute of Research, Division of Neurosciences, Silver
Spring, MD 20910, USA. spcanthony.williams@na.amedd.army.mil
PY - 2003
SN - 0304-3940 (Print)
SP - 147-50
ST - Central neuro-inflammatory gene response following soman exposure in the rat
T2 - Neuroscience letters
TI - Central neuro-inflammatory gene response following soman exposure in the rat
VL - 349
Y2 - 10 y3 - 9
ID - 10288
ER -
TY - JOUR
AB - The revolutionary role of tissue adhesives in wound closure, tissue sealing,
and bleeding control necessitates the development of multifunctional materials
capable of effective and scarless healing. In contrast to the use of traditionally
utilized toxic oxidative crosslinking initiators (exemplified by sodium periodate
and silver nitrate), herein, the natural polyphenolic compound tannic acid (TA) was
used to achieve near instantaneous (<25s), hydrogen bond mediated gelation of
citrate-based mussel-inspired bioadhesives combining anti-oxidant, anti-
inflammatory, and antimicrobial activities (3A-TCMBAs). The resulting materials
were self-healing and possessed low swelling ratios (<60%) as well as considerable
mechanical strength (up to ∼1.0 MPa), elasticity (elongation ∼2700%), and adhesion
(up to 40 kPa). The 3A-TCMBAs showed strong in vitro and in vivo anti-oxidant
ability, favorable cytocompatibility and cell migration, as well as photothermal
antimicrobial activity against both Staphylococcus aureus and Escherichia coli
(>90% bacterial death upon near-infrared (NIR) irradiation). In vivo evaluation in
both an infected full-thickness skin wound model and a rat skin incision model
demonstrated that 3A-TCMBAs + NIR treatment could promote wound closure and
collagen deposition and improve the collagen I/III ratio on wound sites while
simultaneously inhibiting the expression of pro-inflammatory cytokines. Further,
phased angiogenesis was observed via promotion in the early wound closure phases
followed by inhibition and triggering of degradation & remodeling of the
extracellular matrix (ECM) in the late stage (supported by phased CD31 (platelet
endothelial cell adhesion molecule-1) PDGF (platelet-derived growth factor) and
VEGF (vascular endothelial growth factor) expression as well as elevated matrix
metalloprotein-9 (MMP-9) expression on day 21), resulting in scarless wound
healing. The significant convergence of material and bioactive properties
elucidated above warrant further exploration of 3A-TCMBAs as a significant, new
class of bioadhesive.
AN - rayyan-553782348
AU - Wu, K.
AU - Fu, M.
AU - Zhao, Y.
AU - Gerhard, E.
AU - Li, Y.
AU - Yang, J.
AU - Guo, J.
DO - 10.1016/j.bioactmat.2022.05.017
J2 - Bioact Mater
KW - Wound Healing
LA - eng
N1 - Department of Histology and Embryology, NMPA Key Laboratory for Safety
Evaluation of Cosmetics, School of Basic Medical Sciences, Guangdong Provincial Key
Laboratory of Bone and Joint Degeneration Diseases, The Third Affiliated Hospital
of Southern Medical University, Southern Medical University, Guangzhou, China.;
Department of Histology and Embryology, NMPA Key Laboratory for Safety Evaluation
of Cosmetics, School of Basic Medical Sciences, Guangdong Provincial Key Laboratory
of Bone and Joint Degeneration Diseases, The Third Affiliated Hospital of Southern
Medical University, Southern Medical University, Guangzhou, China.; Department of
Histology and Embryology, NMPA Key Laboratory for Safety Evaluation of Cosmetics,
School of Basic Medical Sciences, Guangdong Provincial Key Laboratory of Bone and
Joint Degeneration Diseases, The Third Affiliated Hospital of Southern Medical
University, Southern Medical University, Guangzhou, China.; Department of
Biomedical Engineering, Pennsylvania State University, University Park, PA, 16802,
USA.; Department of Histology and Embryology, NMPA Key Laboratory for Safety
Evaluation of Cosmetics, School of Basic Medical Sciences, Guangdong Provincial Key
Laboratory of Bone and Joint Degeneration Diseases, The Third Affiliated Hospital
of Southern Medical University, Southern Medical University, Guangzhou, China.;
Department of Biomedical Engineering, Pennsylvania State University, University
Park, PA, 16802, USA.; Department of Histology and Embryology, NMPA Key Laboratory
for Safety Evaluation of Cosmetics, School of Basic Medical Sciences, Guangdong
Provincial Key Laboratory of Bone and Joint Degeneration Diseases, The Third
Affiliated Hospital of Southern Medical University, Southern Medical University,
Guangzhou, China.
PY - 2023
SP - 93-110
ST - Anti-oxidant anti-inflammatory and antibacterial tannin-crosslinked citrate-
based mussel-inspired bioadhesives facilitate scarless wound healing
T2 - Bioactive materials
TI - Anti-oxidant anti-inflammatory and antibacterial tannin-crosslinked citrate-
based mussel-inspired bioadhesives facilitate scarless wound healing
VL - 20
Y2 - 2
ID - 10256
ER -
TY - JOUR
AB - Graphene is a novel two-dimensional nanomaterial with a growing number of
practical applications across numerous fields. In this work, we explored potential
biomedical applications of graphene oxide (GO) by systematically studying
antibacterial capacity of GO in both macrophages and animal models. Three types of
bacteria, including Klebsiella pneumoniae (Kp), Escherichia coli (E. coli) and P.
aeruginosa (Pa) were used for in vitro study. Kp was also selected as a
representative multidrug resistant (MDR) bacterium for in vivo study. In in vitro
study, GO effectively eradicated Kp in agar dishes and thus protected alveolar
macrophages (AM) from Kp infection in the culture. In the in vivo evaluation, GO
were introduced intranasally into mouse lungs followed by testing organ tissue
damage including lung, liver, spleen, and kidneys, polymorphonuclear neutrophil
(PMN) penetration, bacterial dissemination, and mortality in Kp-infected mice. We
found that GO can prohibit the growth and spread of Kp both in vitro and in vivo,
resulting in significantly increased cell survival rate, less tissue injury,
subdued inflammatory response, and prolonged mice survival. These findings indicate
that GO could be a promising biomaterial for effectively controlling MDR pathogens.
AN - rayyan-553781607
AU - Wu, X.
AU - Tan, S. R.
AU - Xing, Y. Q.
AU - Pu, Q. Q.
AU - Wu, M.
AU - Zhao, J. X. J.
DO - 10.1016/j.colsurfb.2017.05.024
KW - Drug Resistance, Multiple
Drug Resistance
Bacteria
PY - 2017
SN - 0927-7765 1873-4367
SP - 1-9
ST - Graphene oxide as an efficient antimicrobial nanomaterial for eradicating
multi-drug resistant bacteria in vitro and in vivo
TI - Graphene oxide as an efficient antimicrobial nanomaterial for eradicating
multi-drug resistant bacteria in vitro and in vivo
VL - 157
Y2 - 9 y3 - 1
ID - 9545
ER -
TY - CHAP
AB - It is well known that polymer materials have been extensively investigated
and developed for various biomedical applications due to their palpable advantages,
including availability, good biocompatibility and biodegradability, ordinary
synthesis and characterizations, viable structure modularization, and simple self-
assemble process. Hybrid composition can gain many positive features from both
mixing materials, which are able to meet the requirements in the applications.
Encapsulation of metal nanoparticles in polymer matrices can serve many purposes:
(1) improving stability of metal nanoparticles; (2) reducing toxicity; (3) easy to
be multi-functionalized; and (4) improving collective properties. Silver
nanoparticles (AgNPs) have attracted increasing attention in general and specially
in biomaterial applications. AgNPs have prominent antimicrobial, anticancer,
antiviral, antioxidant, anti-inflammatory and wound healing effects. The biosafety
of AgNPs is a critical issue. Therefore, hybrid nanomaterials based on AgNPs and
polymers are highly significant structures since they integrate synergistically the
advantageous physical-chemical and biological properties of both AgNPs and
polymeric components, providing excellent functionality to the final material. This
chapter critically outlines AgNPs/polymer nanocomposites for various biomedical
applications such as antibacterial, anticancer, tissue engineering, wound healing
and antiviral applications. © 2021, Springer Nature Switzerland AG.
AN - rayyan-553781608
AU - Wu, Y.
DO - 10.1007/978-3-030-44259-0_9
KW - AgNPs/polymer nanocomposites
Antibacterial
Anticancer
Antiviral
Biomedical application
Tissue engineering
Wound healing
Biocompatibility
Biodegradability
Biodegradable polymers
Hybrid materials
Metal nanoparticles
Modular construction
Nanocomposites
Tissue
AgNP/polymer nanocomposite
Antibacterials
Antivirals
Polymer materials
Polymer nanocomposite
Polymer-nanocomposite
Tissues engineerings
Polymerization
Polymers
PY - 2021
SP - 213-246
ST - An Overview of Applications of Silver-Based Polymer Nano Composite as
Biomaterials
T2 - Engineering Materials
TI - An Overview of Applications of Silver-Based Polymer Nano Composite as
Biomaterials
85126707104&doi=10.1007%2f978-3-030-44259-
0_9&partnerID=40&md5=b49504c71a81555eb6924645894fe1cf
ID - 9546
ER -
TY - JOUR
AB - Exposure to inhaled anthropogenic nanomaterials (NM) with dimension <100 nm
has been implicated in numerous adverse respiratory outcomes. Although studies have
identified key NM physiochemical determinants of pneumonic nanotoxicity, the
complex interactive and cumulative effects of NM exposure, especially in
individuals with preexisting inflammatory respiratory diseases, remain unclear.
Herein, the susceptibility of primary human small airway epithelial cells (SAEC)
exposed to a panel of reference NM, namely, CuO, ZnO, mild steel welding fume
(MSWF), and nanofractions of copier center particles (Nano-CCP), is examined in
normal and tumor necrosis factor alpha (TNF-alpha)-induced inflamed SAEC. Compared
to normal SAEC, inflamed cells display an increased susceptibility to NM-induced
cytotoxicity by 15-70% due to a higher basal level of intracellular reactive oxygen
species (ROS). Among the NM screened, ZnO, CuO, and Nano-CCP are observed to
trigger an overcompensatory response in normal SAEC, resulting in an increased
tolerance against subsequent oxidative insults. However, the inflamed SAEC fails to
adapt to the NM exposure due to an impaired nuclear factor erythroid 2-related
factor 2 (Nrf2)-mediated cytoprotective response. The findings reveal that
susceptibility to pulmonary nanotoxicity is highly dependent on the interplay
between NM properties and inflammation of the alveolar milieu.
AN - rayyan-553781609
AU - Wu, Z. R.
AU - Shi, P. J.
AU - Lim, H. K.
AU - Ma, Y. Y.
AU - Setyawati, M. I.
AU - Bitounis, D.
AU - Demokritou, P.
AU - Ng, K. W.
AU - Tay, C. Y.
DO - 10.1002/smll.202000963
IS - 21
KW - Humanities
Humanism
Humans
Epithelial Cells
Inflammation
PY - 2020
SN - 1613-6810 1613-6829
ST - Inflammation Increases Susceptibility of Human Small Airway Epithelial Cells
to Pneumonic Nanotoxicity
T2 - SMALL
TI - Inflammation Increases Susceptibility of Human Small Airway Epithelial Cells
to Pneumonic Nanotoxicity
VL - 16
Y2 - 5
ID - 9547
ER -
TY - JOUR
AB - Biofilms play a key role in the development of chronic tissue infections and
antimicrobial resistance. In situ-forming thermosensitive hydrogel containing
biosynthesized silver nanoparticles using aqueous Eucalyptus camaldulensis leaf
extract (bio-AgNPs) was developed as an efficient anti-biofilm agent with a
sustained release for tissue infection treatment. The formulation with 24%
poloxamer 407, 5% poloxamer 188, and 1% hydroxypropyl methylcellulose demonstrated
gelation temperature at 33 °C and homogeneous dispersion of bio-AgNPs throughout
three-dimensional networks. Pharmaceutical properties of bio-AgNPs-loaded hydrogel
presented shear-thinning behavior and zero-order release profile. The formulation
showed broad-spectrum antimicrobial activity and comparable effects with existing
antibiotics against important pathogens, including Gram-positive, Gram-negative
bacteria, and fungi. The formulation at 1/8–1/2 minimum inhibitory concentrations
(MIC) significantly inhibited biofilm production in all tested pathogens (p <
0.05). Confocal laser scanning microscopy images clearly illustrated dead cells
within mature biofilm upon the treatment with the formulation at 2MIC for 4 h. The
formulation effectively scavenged free radicals and decreased nitric oxide
production up to 98%. Decrease in mRNA levels of inflammation-related enzymes and
pro-inflammatory cytokines in lipopolysaccharide-stimulated RAW 264.7 cells
confirmed the formulation as a potential anti-inflammatory agent. The findings
suggested that bio-AgNPs-loaded hydrogel represents a promising therapeutic
approach for tissue infections. © 2022
AN - rayyan-553781610
AU - Wunnoo, S.
AU - Bilhman, S.
AU - Waen‐ngoen, T.
AU - Yawaraya, S.
AU - Paosen, S.
AU - Lethongkam, S.
AU - Kaewnopparat, N.
AU - Voravuthikunchai, S. P.
DO - 10.1016/j.jddst.2022.103588
Antibiofilm
Antimicrobial
Eucalyptus camaldulensis
Nanocomposite hydrogel
Eucalyptus camaldulensis extract
free radical
hydrogel
hydroxypropylmethylcellulose
lipopolysaccharide
messenger RNA
nitric oxide
plant extract
poloxamer
silver nanoparticle
unclassified drug
animal cell
Article
biosynthesis
controlled study
cross linking
cytotoxicity
dispersion
drug formulation
flow kinetics
freeze thawing
fungus
gelation
gene expression
heat sensitivity
in vitro study
infection resistance
inflammation
nonhuman
plant leaf
pore size
pulp and paper industry
RAW 264.7 cell line
reaction temperature
surface property
PY - 2022
ST - Thermosensitive hydrogel loaded with biosynthesized silver nanoparticles
using Eucalyptus camaldulensis leaf extract as an alternative treatment for
microbial biofilms and persistent cells in tissue infections
T2 - Journal of Drug Delivery Science and Technology
TI - Thermosensitive hydrogel loaded with biosynthesized silver nanoparticles
using Eucalyptus camaldulensis leaf extract as an alternative treatment for
microbial biofilms and persistent cells in tissue infections
85134784171&doi=10.1016%2fj.jddst.2022.103588&partnerID=40&md5=f1d8623006d5e8ec6e4a
99e63a8d56a8
VL - 74
ID - 9548
ER -
TY - JOUR
AB - The development of nanotechnology in the last two decades has led to the use
of silver nanoparticles (AgNPs) in various biomedical applications, including
antimicrobial, anti-inflammatory, and anticancer therapies. However, the potential
of the medical application of AgNPs depends on the safety of their use. In this
work, we assessed the in vitro cytotoxicity and genotoxicity of silver
nanoparticles and identified biomolecules covering AgNPs synthesized from
actinobacterial strain SH11. The cytotoxicity of AgNPs against MCF-7 human breast
cancer cell line and murine macrophage cell line RAW 264.7 was studied by MTT
assay, cell LDH (lactate dehydrogenase) release, and the measurement of ROS
(reactive oxygen species) level while genotoxicity in Salmonella typhimurium cells
was testing using the Ames test. The in vitro analysis showed that the tested
nanoparticles demonstrated dose-dependent cytotoxicity against RAW264.6 macrophages
and MCF-7 breast cancer cells. Moreover, biosynthesizedAgNPsdid not show a
mutagenic effect of S. typhimurium. The analyses and identification of biomolecules
present on the surface of silver nanoparticles showed that they were associated
with proteins. The SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel
electrophoresis) analysis revealed the presence of 34 and 43 kDa protein bands. The
identification of proteins performed by using LC-MS/MS (liquid chromatography with
tandem mass spectrometry) demonstrated their highest homology to bacterial porins.
Capping biomolecules of natural origin may be involved in the synthesis process of
AgNPs or may be responsible for their stabilization. Moreover, the presence of
natural proteins on the surface of bionanoparticles eliminates the postproduction
steps of capping which is necessary for chemical synthesis to obtain the stable
nanostructures required for application in medicine. © 2020 MDPI AG. All rights
reserved.
AN - rayyan-553781611
AU - Wypij, M.
AU - Jedrzejewski, T.
AU - Ostrowski, M.
AU - Trzcinska, J.
AU - Rai, M.
AU - Golinska, P.
DO - 10.3390/molecules25133022
IS - 13
KW - Biosynthesis
Capping proteins
Cytotoxicity
Genotoxicity
Actinobacteria
Animals
Bacterial Proteins
Cytotoxins
Humans
MCF-7 Cells
Metal Nanoparticles
Mice
Mutagenicity Tests
RAW 264.7 Cells
Salmonella typhimurium
Silver
bacterial protein
cytotoxin
metal nanoparticle
silver
animal
chemistry
dose response
drug effect
genetics
human
MCF-7 cell line
metabolism
mouse
mutagen testing
procedures
RAW 264.7 cell line
PY - 2020
ST - Biogenic silver nanoparticles: Assessment of their cytotoxicity, genotoxicity
and study of capping proteins
T2 - Molecules
TI - Biogenic silver nanoparticles: Assessment of their cytotoxicity, genotoxicity
and study of capping proteins
85087730852&doi=10.3390%2fmolecules25133022&partnerID=40&md5=7e7b784b4d2c53132b746e
87328a376b
VL - 25
ID - 9549
ER -
TY - JOUR
AB - The development of nanotechnology in the last two decades has led to the use
of silver nanoparticles (AgNPs) in various biomedical applications, including
antimicrobial, anti-inflammatory, and anticancer therapies. However, the potential
of the medical application of AgNPs depends on the safety of their use. In this
work, we assessed the in vitro cytotoxicity and genotoxicity of silver
nanoparticles and identified biomolecules covering AgNPs synthesized from
actinobacterial strain SH11. The cytotoxicity of AgNPs against MCF-7 human breast
cancer cell line and murine macrophage cell line RAW 264.7 was studied by MTT
assay, cell LDH (lactate dehydrogenase) release, and the measurement of ROS
(reactive oxygen species) level while genotoxicity in Salmonella typhimurium cells
was testing using the Ames test. The in vitro analysis showed that the tested
nanoparticles demonstrated dose-dependent cytotoxicity against RAW 264.6
macrophages and MCF-7 breast cancer cells. Moreover, biosynthesized AgNPs did not
show a mutagenic effect of S. typhimurium. The analyses and identification of
biomolecules present on the surface of silver nanoparticles showed that they were
associated with proteins. The SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel
electrophoresis) analysis revealed the presence of 34 and 43 kDa protein bands. The
identification of proteins performed by using LC-MS/MS (liquid chromatography with
tandem mass spectrometry) demonstrated their highest homology to bacterial porins.
Capping biomolecules of natural origin may be involved in the synthesis process of
AgNPs or may be responsible for their stabilization. Moreover, the presence of
natural proteins on the surface of bionanoparticles eliminates the postproduction
steps of capping which is necessary for chemical synthesis to obtain the stable
nanostructures required for application in medicine.
AN - rayyan-553781783
AU - Wypij, M.
AU - Jędrzejewski, T.
AU - Ostrowski, M.
AU - Trzcińska, J.
AU - Rai, M.
AU - Golińska, P.
DO - 10.3390/molecules25133022
IS - 13
J2 - Molecules
KW - Actinobacteria/metabolism
Animals
Bacterial Proteins/chemistry/*metabolism
Cytotoxins/toxicity
Humans
L-Lactate Dehydrogenase/metabolism
MCF-7 Cells
Metal Nanoparticles/administration & dosage/*chemistry/*toxicity
Mice
Mutagenicity Tests/methods
RAW 264.7 Cells
Salmonella typhimurium/drug effects/genetics
Silver/chemistry/pharmacology
LA - eng
N1 - Department of Microbiology, Nicolaus Copernicus University, Lwowska 1, 87100
Torun, Poland.; Department of Immunology, Nicolaus Copernicus University, Lwowska
1, 87100 Torun, Poland.; Department of Biochemistry, Nicolaus Copernicus
University, Lwowska 1, 87100 Torun, Poland.; Department of Microbiology, Nicolaus
Copernicus University, Lwowska 1, 87100 Torun, Poland.; Nanobiotechnology Lab.,
Department of Biotechnology, SGB Amravati University, Amravati, Maharashtra 444602,
India.; Department of Microbiology, Nicolaus Copernicus University, Lwowska 1,
87100 Torun, Poland.
PY - 2020
ST - Biogenic Silver Nanoparticles: Assessment of Their Cytotoxicity, Genotoxicity
and Study of Capping Proteins
TI - Biogenic Silver Nanoparticles: Assessment of Their Cytotoxicity, Genotoxicity
and Study of Capping Proteins
VL - 25
Y2 - 7 y3 - 2
ID - 9714
ER -
TY - JOUR
AB - Nanoparticles (NPs) interact with biomolecules by forming a biocorona (BC) on
their surface after introduction into the body and alter cell interactions and
toxicity. Metabolic syndrome (MetS) is a prevalent condition and enhances
susceptibility to inhaled exposures. We hypothesize that distinct NP-biomolecule
interactions occur in the lungs due to MetS resulting in the formation of unique
NP-BCs contributing to enhanced toxicity. Bronchoalveolar lavage fluid (BALF) was
collected from healthy and MetS mouse models and used to evaluate variations in the
BC formation on 20 nm iron oxide (Fe3O4) NPs. Fe3O4 NPs without or with BCs were
characterized for hydrodynamic size and zeta potential. Unique and differentially
associated proteins and lipids with the Fe3O4 NPs were identified through proteomic
and lipidomic analyses to evaluate BC alterations based on disease state. A mouse
macrophage cell line was utilized to examine alterations in cell interactions and
toxicity due to BCs. Exposures to 6.25, 12.5, 25, and 50 mu g/mL of Fe3O4 NPs with
BCs for 1 h or 24 h did not demonstrate overt cytotoxicity. Macrophages
increasingly associated Fe3O4 NPs following addition of the MetS BC compared to the
healthy BC. Macrophages exposed to Fe3O4 NPs with a MetS-BC for 1 h or 24 h at a
concentration of 25 mu g/mL demonstrated enhanced gene expression of inflammatory
markers: CCL2, IL-6, and TNF-alpha compared to Fe3O4 NPs with a healthy BC. Western
blot analysis revealed activation of STAT3, NF-kappa B, and ERK pathways due to the
MetS-BC. Specifically, the Jak/Stat pathway was the most upregulated inflammatory
pathway following exposure to NPs with a MetS BC. Overall, our study suggests the
formation of distinct BCs due to NP exposure in MetS, which may contribute to
exacerbated inflammatory effects and susceptibility.
AN - rayyan-553781612
AU - Xia, L.
AU - Alqahtani, S.
AU - Ferreira, C. R.
AU - Aryal, U. K.
AU - Biggs, K.
DO - 10.3390/nano12122022
IS - 12
KW - Oxalic Acid
Norisoprenoids
PY - 2022
SN - 2079-4991
ST - Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in
Iron Oxide Nanoparticle-Biocorona Composition
T2 - NANOMATERIALS
TI - Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in
Iron Oxide Nanoparticle-Biocorona Composition
VL - 12
Y2 - 6
ID - 9550
ER -
TY - JOUR
AB - Bacterial infection has become one of the most challenges for wound healing,
which causes serious inflammatory response and delays the healing process. Herein,
a novel sponge with excellent biocompatible, antibacterial and anti-inflammatory
properties based on quaternized cellulose (QC), sodium alginate (SA) and Zn2+ was
reported. The existence of physical interactions, such as electrostatic
interaction, chelation and hydrogen bonding endowed the sponges with enhanced
mechanical property. The composite sponges exhibited outstanding biocompatibility
and hemostatic efficiency due to the compatible nature of the component and
physical crosslinking, as well as superior antibacterial property benefited from
the synergistic effects of steady Zn2+ release and quaternary ammonium group. In
vivo investigation validated that the enhanced antibacterial and antiinflammatory
effect of the sponges, which significantly promoted wound closure and the
reconstruction of skin tissue through epithelial regeneration, collagen deposition
and mitigating inflammatory cell infiltration. Overall, the novel sponge
demonstrated great potentials in bacteria-associated wound management.
AN - rayyan-553781613
AU - Xie, H. X.
AU - Xia, H. Y.
AU - Huang, L.
AU - Zhong, Z. B. A.
AU - Ye, Q. F.
AU - Zhang, L. N.
AU - Lu, A.
DO - 10.1016/j.ijbiomac.2021.09.047
KW - Wound Healing
Cellulose
Zinc
PY - 2021
SN - 0141-8130 1879-0003
SP - 27-39
ST - Biocompatible, antibacterial and anti-inflammatory zinc ion cross-linked
quaternized cellulose-sodium alginate composite sponges for accelerated wound
healing
TI - Biocompatible, antibacterial and anti-inflammatory zinc ion cross-linked
quaternized cellulose-sodium alginate composite sponges for accelerated wound
healing
VL - 191
Y2 - 11 y3 - 30
ID - 9551
ER -
TY - JOUR
AB - Citrate-modified silver nanoparticles (AgNP-cit) have received extensive
attention due to their excellent antimicrobial properties. However, these particles
tend to migrate in vivo, thereby entering the blood circulatory system in granular
form and accumulating in the liver, causing toxic reactions. However, the mechanism
underlying AgNP-cit toxicity is not yet clear. Thus, we adopted a tandem mass tag
(TMT)-labeled quantitative proteomics and metabolomics approach to identify
proteins and small molecule metabolites associated with AgNP-cit-induced liver
damage and constructed interaction networks between the differentially expressed
proteins and metabolites to explain the AgNP-cit toxicity mechanism. AgNP-cit
resulted in abnormal purine metabolism mainly by affecting xanthine and other key
metabolites along with pyruvate kinase and other bodily proteins, leading to
oxidative stress. AgNP-cit regulated the metabolism of amino acids and glycerol
phospholipids through glycerol phospholipids, CYP450 enzymes and other key
proteins, causing liver inflammation. Via alanine, isoleucine, L-serine
dehydratase/L-threonine deaminase and other proteins, AgNP-cit altered the
metabolism of glycine, serine and threonine, cysteine and methionine, affecting
oxidation and deamination, and ultimately leading to liver damage. This work
clearly explains toxic reactions induced by AgNP-cit from three perspectives,
oxidative stress, inflammatory response, and oxidation and deamination, thus
providing an experimental basis for the safe application of nanomaterials. © 2017
AN - rayyan-553781615
AU - Xie, J.
AU - Dong, W.
AU - Liu, R.
AU - Wang, Y.
AU - Li, Y.
DO - 10.1080/17435390.2017.1415389
IS - 1
KW - Citrate-modified silver nanoparticles
systems biology analysis
UPLC/Q-TOF-MS
Animals
Citric Acid
Male
Metabolome
Metabolomics
Metal Nanoparticles
Oxidative Stress
Proteome
Proteomics
Rats
Silver
alanine
amino acid
biological marker
citric acid
cysteine
cytochrome P450
glycerol
glycine
isoleucine
methionine
phospholipid
pyruvate kinase
serine
serine dehydratase
silver nanoparticle
threonine
threonine ammonia lyase
xanthine
metal nanoparticle
proteome
silver
amino acid metabolism
animal experiment
animal model
animal tissue
Article
controlled study
deamination
histopathology
inflammation
liver injury
liver toxicity
male
metabolite
metabolomics
nonhuman
oxidation
oxidative stress
pathogenesis
priority journal
protein expression
protein metabolism
proteomics
rat
tandem mass tag
time of flight mass spectrometry
animal
chemistry
drug effect
metabolism
metabolome
toxic hepatitis
PY - 2018
SP - 18-31
ST - Research on the hepatotoxicity mechanism of citrate-modified silver
nanoparticles based on metabolomics and proteomics
T2 - Nanotoxicology
TI - Research on the hepatotoxicity mechanism of citrate-modified silver
nanoparticles based on metabolomics and proteomics
85038371059&doi=10.1080%2f17435390.2017.1415389&partnerID=40&md5=6107e2b8f9582abaaf
601204a4e434ba
VL - 12
ID - 9553
ER -
TY - JOUR
AB - Background: Drug resistance of pathogens and immunosuppression are the main
causes of clinical stagnation of osteomyelitis. The ideal treatment strategy for
osteomyelitis is to achieve both efficient antibacterial and bone healing through
spatiotemporal modulation of immune microenvironment. Methods: In this study, a
bilayer hydrogel based on genetically engineered polypeptide AC10A and AC10ARGD was
prepared by self-assembly. Ag2S QDs@DSPE-mPEG2000-Ce6/Aptamer (AD-Ce6/Apt) was
loaded in the top layer AC10A hydrogel (AA) for antibacterial, and bone marrow-
derived mesenchymal stem cells (BMSCs) were loaded in the lower layer AC10ARGD
hydrogel (MAR) for bone healing. The AD-Ce6/Apt can be released from the AA
hydrogel to target S. aureus before bacterial biofilm formation and achieved
significant bactericidal effect under irradiation with a 660 nm laser. Moreover,
AD-Ce6/Apt can induce M1 type polarization of macrophages to activate the immune
system and eliminate residual bacteria. Subsequently, BMSCs released from the MAR
hydrogel can differentiate into osteoblasts and promote the formation of an anti-
inflammatory microenvironment by regulating the M2 type polarization of
macrophages. The bilayer AA-MAR hydrogel possessed good biocompatibility. Results:
The in vitro and in vivo results showed that the AA-MAR hydrogel not only realized
efficient photodynamic therapy of S. aureus infection, but also promoted the
transformation of immune microenvironment to fulfill the different needs of each
stage, which ultimately improved bone regeneration and mechanical properties post-
surgery. Conclusion: This work presents an approach for spatiotemporal modulation
of immune microenvironment in the treatment of osteomyelitis. Graphical Abstract:
[Figure not available: see fulltext.]. © 2022, The Author(s).
AN - rayyan-553781617
AU - Xie, X.
AU - Wei, J.
AU - Zhang, B.
AU - Xiong, W.
AU - He, Z.
AU - Zhang, Y.
AU - Gao, C.
AU - Zhao, Y.
AU - Liu, B.
DO - 10.1186/s12951-022-01614-3
IS - 1
KW - Antibacterial
Bone repairing
Engineered polypeptide hydrogel
Osteomyelitis
Spatiotemporal regulation
Dimaprit
Humans
Hydrogels
Peptides
Biocompatibility
Macrophages
Modulation
Photodynamic therapy
Polarization
Silver compounds
Stem cells
Tissue regeneration
1,2 distearoyl sn glycero 3 phosphoethanolamine
aptamer
chlorin e6
glycerophospholipid
hydrogel
interleukin 6
macrogol 2000
photosensitizing agent
polypeptide
polypeptide AC10A
polypeptide AC10ARGD
quantum dot
silver derivative
silver sulfide
unclassified drug
antiinfective agent
APT
dimaprit
peptide
Antibacterials
Aptamers
Bi-layer
Bone healing
Microenvironments
Polypeptide hydrogels
animal cell
animal experiment
animal model
animal tissue
Article
bacterial clearance
biocompatibility
bioengineering
biofilm
bone marrow derived macrophage
bone marrow mesenchymal stem cell
bone regeneration
bone remodeling
controlled study
drug degradation
drug release
fracture healing
immune system
immunomodulation
in vitro study
in vivo study
laser therapy
M1 macrophage
M2 macrophage
microenvironment
nonhuman
osteoblast
osteomyelitis
photodynamic therapy
polarization
rat
spatiotemporal analysis
Staphylococcus aureus infection
chemistry
human
Cell culture
Inflammation
PY - 2022
ST - A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal
modulation of bactericidal and anti-inflammation process in osteomyelitis treatment
TI - A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal
modulation of bactericidal and anti-inflammation process in osteomyelitis treatment
85137910944&doi=10.1186%2fs12951-022-01614-
3&partnerID=40&md5=abb89cd8273dc828bb11e2263344e94e
VL - 20
ID - 9555
ER -
TY - JOUR
AB - Graphene, including graphene quantum dots, its oxide and unoxidized forms
(pure graphene) have several properties, like fluorescence, electrical
conductivity, theoretical surface area, low toxicity, and high biocompatibility. In
this study, we evaluated genotoxicity (in silico analysis using the functional
density theory-FDT), cytotoxicity (human glioblastoma cell line), in vivo
pharmacokinetics, in vivo impact on microcirculation and cell internalization
assay. It was also radiolabeled with lutetium 177 (177Lu), a beta emitter
radioisotope to explore its therapeutic use as nanodrug. Finally, the impact of its
disposal in the environment was analyzed using ecotoxicological evaluation. FDT
analysis demonstrated that graphene can construct covalent and non-covalent bonds
with different nucleobases, and graphene oxide is responsible for generation of
reactive oxygen species (ROS), corroborating its genotoxicity. On the other hand,
non-cytotoxic effect on glioblastoma cells could be demonstrated. The
pharmacokinetics analysis showed high plasmatic concentration and clearance.
Topical application of 0.1 and 1 mg/kg of graphene nanoparticles on the hamster
skinfold preparation did not show inflammatory effect. The cell internalization
assay showed that 1-hour post contact with cells, graphene can cross the plasmatic
membrane and accumulate in the cytoplasm. Radio labeling with 177Lu is possible and
its use as therapeutic nanosystem is viable. Finally, the ecotoxicity analysis
showed that A. silina exposed to graphene showed pronounced uptake and absorption
in the nauplii gut and formation of ROS. The data obtained showed that although
being formed exclusively of carbon and carbon-oxygen, graphene and graphene oxide
respectively generate somewhat contradictory results and more studies should be
performed to certify the safety use of this nanoplatform.
AN - rayyan-553781618
AU - Xing, H. X.
AU - de Barros, A. O. D.
AU - Mello, Fdce
AU - Sozzi-Guo, F.
AU - Muller, C.
AU - Gemini-Piperni, S.
AU - Alencar, L. M. R.
AU - Maia, F. F.
AU - Freire, V. N.
AU - de Menezes, F. D.
AU - Aran, V.
AU - Devalle, S.
AU - Moura-Neto, V.
AU - Ricci, E.
AU - Bouskela, E.
AU - Pikula, K.
AU - Golokhvast, K.
AU - Santos-Oliveira, R.
DO - 10.1166/jbn.2021.3006
IS - 1
KW - Graphite
PY - 2021
SN - 1550-7033 1550-7041
SP - 131-148
ST - Graphene: Insights on Biological, Radiochemical and Ecotoxicological Aspects
TI - Graphene: Insights on Biological, Radiochemical and Ecotoxicological Aspects
VL - 17
Y2 - 1
ID - 9556
ER -
TY - JOUR
AB - Burn infection delays wound healing and increases the burn patient mortality.
Consequently, a new dressing with antibacterial and anti-inflammatory dual
properties is urgently required for wound healing. In this study, we propose a
combination of methacrylate gelatin (GelMA) hydrogel system with silver
nanoparticles embed in γ-cyclodextrin metal–organic frameworks (Ag@MOF) and
hyaluronic acid-epigallocatechin gallate (HA-E) for the burn wound infection
treatment. Ag@MOF is used as an antibacterial agent and epigallocatechin gallate
(EGCG) has exhibited biological properties of anti-inflammation and antibacterial.
The GelMA/HA-E/Ag@MOF hydrogel enjoys suitable physical properties and sustained
release of Ag+. Meanwhile, the hydrogel has excellent biocompatibility and could
promote macrophage polarization from M1 to M2. In vivo wound healing evaluations
further demonstrate that the GelMA/HA-E/Ag@MOF hydrogel reduces the number of the
bacterium efficiently, accelerates wound healing, promotes early angiogenesis, and
regulates immune reaction. A further evaluation indicates that the noncanonical Wnt
signal pathway is significantly activated in the GelMA/HA-E/Ag@MOF hydrogel treated
group. In conclusion, the GelMA/HA-E/Ag@MOF hydrogel could serve as a promising
multifunctional dressing for the burn wound healing. © 2022 The Authors.
Bioengineering & Translational Medicine published by Wiley Periodicals LLC on
behalf of American Institute of Chemical Engineers.
AN - rayyan-553781622
AU - Xiong, Y.
AU - Xu, Y.
AU - Zhou, F.
AU - Hu, Y.
AU - Zhao, J.
AU - Liu, Z.
AU - Zhai, Q.
AU - Qi, S.
AU - Zhang, Z.
AU - Chen, L.
DO - 10.1002/btm2.10373
IS - 1
KW - Ag-metal–organic framework
burn wound infection
macrophage polarization
methacrylate gelatin
Biocompatibility
Hyaluronic acid
Hydrogels
Macrophages
Metal nanoparticles
alkali metal
antiinfective agent
collagen
cyclodextrin
gamma cyclodextrin
gelatin
hyaluronic acid
hyaluronidase
hydrogel
pentobarbital
silver nanoparticle
Anti-inflammatories
Antibacterials
Burn wound infection
Epigallocatechin gallate
Macrophage polarization
Methacrylate gelatin
Wound healing
Wound infections
3T3 cell line
angiogenesis
animal cell
animal experiment
animal model
animal tissue
Article
bacterial growth
biocompatibility
blood flow
burn infection
cell adhesion assay
cell migration
cell proliferation
chemoluminescence
coculture
colony forming unit
controlled study
dispersity
epithelium cell
Escherichia coli
foreign body giant cell
freeze drying
hemostasis
histology
inflammation
macrophage
nonhuman
polarization
pore size
rat
RAW 264.7 cell line
Western blotting
Wnt signaling
wound healing
wound infection
Polarization
Burns
Tocopherols
PY - 2023
ST - Bio-functional hydrogel with antibacterial and anti-inflammatory dual
properties to combat with burn wound infection
T2 - Bioengineering and Translational Medicine
TI - Bio-functional hydrogel with antibacterial and anti-inflammatory dual
properties to combat with burn wound infection
85133365479&doi=10.1002%2fbtm2.10373&partnerID=40&md5=203119a3f5d4c4e91de859cf608ce
95b
VL - 8
ID - 9560
ER -
TY - JOUR
AB - In this study, an eco-friendly and low-cost procedure for the in situ
fabrication of Cu nanoparticles by using chitosan/alginate hydrogel. The prepared
Cu NPs@CS/Alg nanocomposite were characterized by advanced physicochemical
techniques like Fourier Transformed Infrared spectroscopy (FT-IR), Scanning
Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy
Dispersive X-ray spectroscopy (EDX) and X-ray Diffraction (XRD) study. It has been
established that chitosan/alginate-capped gold nanoparticles have a spherical shape
with a mean diameter from 10 to 20 nm. In the cellular and molecular part of the
recent study, the treated cells with Cu NPs@CS/Alg nanocomposite were assessed by
MTT assay for 48 h about the cytotoxicity and anti-human breast cancer properties
on normal (HUVEC) and breast cancer cell lines i.e. infiltrating lobular carcinoma
of breast (UACC-3133), inflammatory carcinoma of the breast (UACC-732), and
metastatic carcinoma (MDA-MB-453). In the antioxidant test, the IC50 of Cu
NPs@CS/Alg nanocomposite and BHT against DPPH free radicals were 344 and 193 mu
g/mL, respectively. The IC50 of Cu NPs@CS/Alg nanocomposite were 297, 386, and 359
mu g/ mL against KYSE-270, OE33, and ESO26 cell lines, respectively. The viability
of malignant breast cell line reduced dose-dependently in the presence of Cu
NPs@CS/Alg nanocomposite. (C) 2021 Published by Elsevier B.V. on behalf of King
Saud University.
AN - rayyan-553781623
AU - Xu, D.
AU - Li, E.
AU - Karmakar, B.
AU - Awwad, N. S.
AU - Ibrahium, H. A.
AU - Osman, H. E. H.
AU - El-kott, A. F.
AU - Abdel-Daim, M. M.
DO - 10.1016/j.arabjc.2021.103638
IS - 3
KW - Humanities
Humanism
Humans
Breast Neoplasms
PY - 2022
SN - 1878-5352 1878-5379
ST - Green preparation of copper nanoparticle-loaded chitosan/alginate bio-
composite: Investigation of its cytotoxicity, antioxidant and anti-human breast
cancer properties
TI - Green preparation of copper nanoparticle-loaded chitosan/alginate bio-
composite: Investigation of its cytotoxicity, antioxidant and anti-human breast
cancer properties
VL - 15
Y2 - 3
ID - 9561
ER -
TY - JOUR
AB - AIM: To investigate effect of cytokines in the immunopathogenesis of
Helicobacter pylori (H. pylori). METHODS: Forty-eight patients (37 with chronic
gastritis and 11 with duodenal ulcer (DU), and aged 21-63 yrs) were biopsied under
endoscopy for gastric mucosa culture in vitro and H. pylori detection. Rapid urease
test, H. pylori culture and Warthin-Starry silver stains were used for H. pylori
diagnosis. H. pylori was defined as positive when any 2 of these tests were
positive. Each of 3 antrum pylori biopsies was cultured in vitro for 24 h by adding
1mL RPMI 1640 (37°C, 50mL·L-1 CO2). Supernatants were centrifuged and used for the
detection of interleukin (IL)-6, IL-8 and tumor necrosis factor-a (TNF-α) by ELISA.
Total proteins of the biopsies were assayed by a modified Lowry method. Another 8
cases were cultured with RPMI 1640 added by H. pylori vacuolating cytotoxin (VacA).
The results were expressed as ng orμg per gram protein (ng·g-1 or μg·g-1). RESULTS:
Sixty-nine percent of the patients were H. pylori positive. IL-6, IL-8 and TNF-α
contents in the supernatants of cultured mucosa were all significantly higher in H.
pylori positive patients than those in H. pylori negative patients. Among them, IL-
8 content of 9 cases of DU patients was 53 (18-96) μg·g-1, which was significantly
higher than 36(7-84) μg·g-1 of chronic gastritis patients (P < 0.01). Addition of
VacA into culture media could significantly promote IL-8 secretion from gastric
mucosa (50 ± 38 μg·g-1 vs 68 ± 30 μg·g-1, P < 0.01). TNF-α content was not
significantly increased, and IL-6 was not changed. IL-8 was closely associated with
the inflammation grading and activity (r = 0.98, P < 0.0025). No correlation was
observed between IL-6, TNFα and inflammation grading (r = -0.26 and -0.28, P >
0.25), but they were higher in active gastritis than those in nonactive gastritis.
CONCLUSIONS: IL-8 is closely associated with the infiltration of inflammatory
cells, and H. pylori cytotoxins are responsible for the increased secretion of
cytokines.
AN - rayyan-553781624
AU - Xu, K. Q.
AU - Zhang, W. D.
AU - Wang, J. D.
AU - Li, Z. X.
AU - Zhou, D. Y.
AU - Zhang, Y. L.
AU - Huang, W. F.
AU - Jiang, B.
AU - Sun, Y.
IS - 8
KW - cytokine
cytotoxin
gene product
interleukin 6
interleukin 8
unclassified drug
vacuolating toxin
adult
article
biopsy technique
cancer grading
chronic gastritis
clinical article
correlation analysis
cytokine production
cytotoxicity
duodenum ulcer
female
Helicobacter pylori
human
immunopathogenesis
male
protein content
staining
stomach antrum
stomach mucosa
Gastric Mucosa
Cytotoxins
PY - 2002
SP - 907-911
ST - Cytotoxin of Helicobacter pylori promotes IL-8 secretion of gastric mucosa in
vitro
T2 - World Chinese Journal of Digestology
TI - Cytotoxin of Helicobacter pylori promotes IL-8 secretion of gastric mucosa in
vitro
0036689431&partnerID=40&md5=91e06f2b31a0871b8e35b55453322e52
VL - 10
ID - 9562
ER -
TY - JOUR
AB - Reactive oxygen species (ROS) produced by noble metallic nanoparticles under
visible light is an effective way to combat drug-resistant bacteria colonized on
the wound. However, the photocatalytic efficiency of noble metallic nanoparticles
is limited by its self-aggregation in water media. Moreover, the fast release of
noble metallic ions from nanoparticles might engender cellular toxicity and
hazardous environmental issues. Herein, we chose AgNPs, the most common plasmonic
noble metallic nanoparticles, as an example, modifying the surface of AgNPs with
oleic acid and n-butylamine and imbedded them into calcium alginate (CA) hydrogel
that holds tissue adhesion, rapid hemostatic, sunlight -sensitive antibacterial and
anti-inflammatory abilities, and thus effectively promotes the healing of wounds.
Unlike conventional AgNP-based materials, the constrain of colloids and hydrogel
networks hinders the leach of Ag+. Nonetheless, the CA/Ag hydrogels exhibit on-
demand photodynamic antibacterial efficacy due to the generation of ROS under
visible light. In addition, the CA/Ag hydrogel can effectively stop the hemorrhage
in a mouse liver bleeding model due to their skin-adaptive flexibility and tissue
adhesiveness. The potent sunlight-responsive antibacterial activity of the CA/Ag
hydrogel can effectively kill multidrug-resistant bacteria both in vitro (>99.999%)
and in vivo (>99.9%), while the diminished Ag+ release guarantees its
biocompatibility. The CA/Ag hydrogel significantly promotes the wound healing
process by the downregulation of proinflammatory cytokines (TNF-alpha and IL-6) in
a rodent full-thickness cutaneous wound model. Overall, the proposed
multifunctional CA/Ag nanocomposite hydrogel has excellent prospects as an advanced
wound dressing.
AN - rayyan-553781631
AU - Xu, M.
AU - Ji, X. H.
AU - Huo, J. J.
AU - Chen, J. J.
AU - Liu, N.
AU - Li, Z. Y.
AU - Jia, Q. Y.
AU - Sun, B.
AU - Zhu, M. F.
AU - Li, P.
DO - 10.1021/acsami.3c03247
IS - 14
KW - Wound Healing
PY - 2023
SN - 1944-8244 1944-8252
SP - 17742-17756
ST - Nonreleasing AgNP Colloids Composite Hydrogel with Potent Hemostatic,
Photodynamic Bactericidal and Wound Healing- Promoting Properties
TI - Nonreleasing AgNP Colloids Composite Hydrogel with Potent Hemostatic,
Photodynamic Bactericidal and Wound Healing- Promoting Properties
VL - 15
Y2 - 4 y3 - 12
ID - 9569
ER -
TY - JOUR
AB - Open wounds (e.g., burns and trauma) are always challenged by various
opportunistic bacteria. There is an urgent need for developing novel wound dressing
that is able to prevent bacterial infection and promote the healing simultaneously.
Herein, we developed a new type of antimicrobial hydrogels for the open wound
healing through imitating a facile mussel-inspired catechol/polyamine chemistry.
This hydrogel was prepared using catechol (CT) and e-poly-L-lysine (EPL) by
oxidative cross-linking directly in the open air at room temperature. This
nonleaching CT/EPL hydrogel not only exhibited excellent contact-active
antimicrobial activities against Gram-negative bacteria Escherichia coli (E. coli)
and Gram-positive "superbug" methicillin-resistant Staphylococcus aureus (MRSA) but
also inhibited the biofilm formation in vitro. Moreover, the animal burn wound
model study clearly validated the in vivo anti-infective property of CT/EPL
hydrogel against MRSA infection. More importantly, the CT/EPL hydrogel possessed
low cytotoxicity and enhanced cell migration in vitro. A full-thickness cutaneous
wound model study revealed that CT/EPL hydrogel upregulated the expression of
vascular endothelial growth factor (VEGF) and reduced the production of the pro-
inflammatory cytokines, thus promoted the wound healing. These findings suggested
that the CT/EPL hydrogel have great potential as a wound dressing for preventing
bacterial infection and accelerating healing of open wounds.
AN - rayyan-553781632
AU - Xu, M.
AU - Khan, A.
AU - Wang, T. J.
AU - Song, Q.
AU - Han, C. M.
AU - Wang, Q. Q.
AU - Gao, L. L.
AU - Huang, X.
AU - Li, P.
AU - Huang, W.
DO - 10.1021/acsabm.9b00353
IS - 8
KW - Wound Healing
PY - 2019
SN - 2576-6422
SP - 3329-3340
ST - Mussel-Inspired Hydrogel with Potent in Vivo Contact-Active Antimicrobial and
Wound Healing Promoting Activities
T2 - ACS APPLIED BIO MATERIALS
TI - Mussel-Inspired Hydrogel with Potent in Vivo Contact-Active Antimicrobial and
Wound Healing Promoting Activities
VL - 2
Y2 - 8 y3 - 19
ID - 9570
ER -
TY - JOUR
AB - As the continuous development of the industrial revolution, nanomaterials
with excellent characteristics have been widely applied in various fields, greatly
increasing the probability of human exposure to nanomaterials and the concerns
about the potential nanotoxicity. Existing studies have shown that the toxicity of
nanomaterials may be closely related to oxidative stress, inflammatory response,
phagocytosis dysfunction, DNA damage, etc. Based on our focus, nanomaterials may
cross the human barrier through various channels and disrupt various cell-cell
junctions, while the integrity of cellular barrier is a necessary for the normal
physiological function of various organs. However, until now, there is still a lack
of systematic discussion in this field. This review illustrates the importance of
cell-cell junctions in maintaining various organ functions and highlights the
mechanism of various nanomaterials disrupt cell-cell junctions, as well as the
possible damage to various organs, such as brain, eye, lung, breast, intestine,
placenta, testis, heart, liver, kidney, skin, etc. Awareness of the potential
negative effects of nanomaterials will help scientists deeply understand the
limitations of nanotechnology, inspiring them to develop safer and more efficient
nanomaterials for future personalized nanomedicine.
AN - rayyan-553781635
AU - Xu, S. B.
AU - Pang, X. L.
AU - Zhang, X. Y.
AU - Lv, Q.
AU - Zhang, M.
AU - Wang, J. P.
AU - Ni, N. Y.
AU - Sun, X.
DO - 10.1007/s12274-023-5455-y
KW - Oxygenators
PY - 2023
SN - 1998-0124 1998-0000
ST - Microenvironment regulation of M-N-C single-atom catalysts towards oxygen
reduction reaction
T2 - NANO RESEARCH
TI - Microenvironment regulation of M-N-C single-atom catalysts towards oxygen
reduction reaction
Y2 - 3 y3 - 16
ID - 9573
ER -
TY - JOUR
AB - As the continuous development of the industrial revolution, nanomaterials
with excellent characteristics have been widely applied in various fields, greatly
increasing the probability of human exposure to nanomaterials and the concerns
about the potential nanotoxicity. Existing studies have shown that the toxicity of
nanomaterials may be closely related to oxidative stress, inflammatory response,
phagocytosis dysfunction, DNA damage, etc. Based on our focus, nanomaterials may
cross the human barrier through various channels and disrupt various cell-cell
junctions, while the integrity of cellular barrier is a necessary for the normal
physiological function of various organs. However, until now, there is still a lack
of systematic discussion in this field. This review illustrates the importance of
cell -cell junctions in maintaining various organ functions and highlights the
mechanism of various nanomaterials disrupt cell -cell junctions, as well as the
possible damage to various organs, such as brain, eye, lung, breast, intestine,
placenta, testis, heart, liver, kidney, skin, etc. Awareness of the potential
negative effects of nanomaterials will help scientists deeply understand the
limitations of nanotechnology, inspiring them to develop safer and more efficient
nanomaterials for future personalized nanomedicine.
AN - rayyan-553781636
AU - Xu, S. B.
AU - Pang, X. L.
AU - Zhang, X. Y.
AU - Lv, Q.
AU - Zhang, M.
AU - Wang, J. P.
AU - Ni, N. Y.
AU - Sun, X.
DO - 10.1007/s12274-023-5455-y
IS - 5
PY - 2023
SN - 1998-0124 1998-0000
SP - 7053-7074
ST - Nanomaterials diseases cell -cell junctions towards various diseases
T2 - NANO RESEARCH
TI - Nanomaterials diseases cell -cell junctions towards various diseases
VL - 16
Y2 - 5
ID - 9574
ER -
TY - JOUR
AB - Nanoparticles (NPs) have become one of the most popular objects of scientific
study during the past decades. However, despite wealth of study reports, still
there is a gap, particularly in health toxicology studies, underlying mechanisms,
and related evaluation models to deeply understanding the NPs risk effects. In this
review, we first present a comprehensive landscape of the applications of NPs on
health, especially addressing the role of NPs in medical diagnosis, therapy. Then,
the toxicity of NPs on health systems is introduced. We describe in detail the
effects of NPs on various systems, including respiratory, nervous, endocrine,
immune, and reproductive systems, and the carcinogenicity of NPs. Furthermore, we
unravels the underlying mechanisms of NPs including ROS accumulation, mitochondrial
damage, inflammatory reaction, apoptosis, DNA damage, cell cycle, and epigenetic
regulation. In addition, the classical study models such as cell lines and mice and
the emerging models such as 3D organoids used for evaluating the toxicity or
scientific study are both introduced. Overall, this review presents a critical
summary and evaluation of the state of understanding of NPs, giving readers more
better understanding of the NPs toxicology to remedy key gaps in knowledge and
techniques.
AN - rayyan-553781637
AU - Xuan, L. H.
AU - Ju, Z.
AU - Skonieczna, M.
AU - Zhou, P. K.
AU - Huang, R. X.
DO - 10.1002/mco2.327
IS - 4
KW - Humanities
Humanism
Humans
PY - 2023
SN - 2688-2663
ST - Nanoparticles-induced potential toxicity on human health: Applications,
toxicity mechanisms, and evaluation models
T2 - MEDCOMM
TI - Nanoparticles-induced potential toxicity on human health: Applications,
toxicity mechanisms, and evaluation models
VL - 4
Y2 - 8
ID - 9575
ER -
TY - JOUR
AB - Objective: To evaluate the biocompatibility of chemically extracted acellular
muscle grafts (CEAM) and to demonstrate its advantages as tissue engineering
scaffold after transplantation into the spinal cords of adult rats. Methods:
Thirty-six male rats were randomly assigned to control group and CEAM group, and
underwent spinal cord lateral hemisection. After preparation of successful models,
treatment consisted of application of CEAM into the lesion gap, which was left
empty in control group. Six rats in each group were killed 1, 2 and 4 weeks after
induction of the injury. Sections were stained for quantification of
microglia/macrophages using ED-1 on week 1, week 2 and week 4. Additionally,
Holmes' silver staining method was chosen to detect axonal regeneration, glial
fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial
scars, and alkaline phosphatase staining for vascularisation on day 28. Results: In
control group, no definite axonal outgrowth into the lesion was found. The
inflammatory response was most pronounced on day 7 and a dense non-oriented
accumulation of astrocytes could be found at the edge of the lesion cavity in
control group. In CEAM group, the number of regenerating axons in the scaffolds was
613.17 ±154.96, and they grew into the grafts in a strikingly organized fashion.
The change of inflammatory response in CEAM group was the same as that in control
group and there was no sign of the foreign body reaction induced by CEAM. In rats
of CEAM group the astrocytes grew into the graft in a diffuse linear array.
Vascularisation of the grafts was also confirmed. Conclusion: CEAM is biocompatible
with spinal cord, and it indicates that CEAM is a kind of good tissue engineering
scaffold for the repair of spinal cord injury.
AN - rayyan-553781638
AU - Xue, H.
AU - Chen, D.
AU - Zhang, X. Y.
AU - Liu, Y.
IS - 5
KW - Acellular muscle
Rats, Wistar
Spinal cord injury
Tissue engineering
Transplantation
animal cell
animal experiment
article
astrocyte
biocompatibility
control group
controlled study
foreign body
graft perfusion
inflammation
macrophage
male
methodology
microglia
muscle graft
nerve cell growth
nerve fiber degeneration
nerve fiber regeneration
nonhuman
quantitative study
rat
spinal cord hemisection
spinal cord injury
staining
tissue engineering
tissue section
vascularization
Rats
Spinal Cord Injuries
Spinal Cord
PY - 2009
SP - 801-804
ST - Biocompatibility of chemically extracted acellular muscle grafts as
biomatrices in experimental spinal cord injury in rats
T2 - Journal of Jilin University Medicine Edition
TI - Biocompatibility of chemically extracted acellular muscle grafts as
biomatrices in experimental spinal cord injury in rats
70350741614&partnerID=40&md5=e15b23e7c58bc02ae668786876ef697f
VL - 35
ID - 9576
ER -
TY - JOUR
AB - This study evaluated the acute toxicity and biokinetics of intravenously
administered silver nanoparticles (AgNPs) in mice. Mice were exposed to different
dosages of AgNPs (7.5, 30 or 120mg kg-1). Toxic effects were assessed via general
behavior, serum biochemical parameters and histopathological observation of the
mice. Biokinetics and tissue distribution of AgNPs were evaluated at a dose of
120mg kg-1 in both male and female mice. Inductively coupled plasma-mass
spectrometry (ICP-MS) was used to determine silver concentrations in blood and
tissue samples collected at predetermined time intervals. After 2weeks, AgNPs
exerted no obvious acute toxicity in the mice. However, inflammatory reactions in
lung and liver cells were induced in mice treated at the 120mg kg-1 dose level. The
highest silver levels were observed in the spleen, followed by liver, lungs and
kidneys. The elimination half-lives and clearance of AgNPs were 15.6h and 1.0ml h-1
g-1 for male mice and 29.9h and 0.8ml h-1 g-1 for female mice. These results
indicated that AgNPs could be distributed extensively to various tissues in the
body, but primarily in the spleen and liver. Furthermore, there appears to be
gender-related differences in the biokinetic profiles in blood and distribution in
lungs and kidneys following an intravenous injection of AgNPs. The data from this
study provides information on toxicity and biodistribution of AgNPs following
intravenous administration in mice, which represents the worst case scenario of
toxicity among all the different administration routes, and may shed light in the
future use of products containing AgNPs in humans. Copyright © 2012 John Wiley &
Sons, Ltd. The acute toxic effects and biokinetics of intravenously administered
silver nanoparticles (AgNPs) were investigated. AgNPs exerted no obvious acute
toxicity when given intravenously in mice at dose levels of 7.5-120mg kg-1. AgNPs
could be distributed extensively to various tissues in the body, and the spleen and
liver were the main target organs. Gender-related differences for the biokinetics
and distribution were noted, and the elimination half-lives were 15.6 and 29.9h for
male and female mice, respectively. © 2012 John Wiley & Sons, Ltd.
AN - rayyan-553781640
AU - Xue, Y.
AU - Zhang, S.
AU - Huang, Y.
AU - Zhang, T.
AU - Liu, X.
AU - Hu, Y.
AU - Zhang, Z.
AU - Tang, M.
DO - 10.1002/jat.2742
IS - 11
KW - Acute toxic effects
Gender-related biokinetics
Mice
Tissue distribution
Animals
Female
Kinetics
Male
Mass Spectrometry
Metal Nanoparticles
Mice, Inbred ICR
Nanoparticles
Sex Factors
Silver
Tissue Distribution
Mus
albumin
cholesterol
creatinine
protein
silver nanoparticle
animal behavior
animal experiment
article
biokinetics
chemical, physical and mathematical phenomena
controlled study
drug blood level
drug tissue level
female
histopathology
inflammation
kidney
liver
liver cell
lung
male
mass spectrometry
mouse
nonhuman
priority journal
sex difference
spleen
Interpersonal Relations
PY - 2012
SP - 890-899
ST - Acute toxic effects and gender-related biokinetics of silver nanoparticles
following an intravenous injection in mice
TI - Acute toxic effects and gender-related biokinetics of silver nanoparticles
following an intravenous injection in mice
84866863235&doi=10.1002%2fjat.2742&partnerID=40&md5=9977b3b848522e950f1e6c286ec0a2e
7
VL - 32
ID - 9577
ER -
TY - JOUR
AB - Diabetes mellitus (Madhumeha) is one of the leading metabolic disorder
prevalent in the developing countries which is characterized by high blood sugar
level and is associated with macrovascular and microvascular complications. The
Indian Ayurveda describes several herbs for the management and treatment of
diabetes mellitus among which Gymnema sylvestre (Asclepiadaceae) is revered as a
potential antidiabetic herbal drug which has the capability of simultaneously
regenerating β-cell and stimulating insulin secretion. Gymnema sylvestre also
possesses anti-obesity, anti-hyperlipidemic, anti-inflammatory, and anti-cancerous
activities. This review updates the recent developments in the experimental studies
conducted on the Gymnema sylvestre as an effective remedy for diabetes mellitus
evidenced by both animals and human studies. Moreover, this study also discussed
the toxicity of Gymnema sylvestre and future challenges in the roadmap of
formulation for prevention and control of diabetes. ©Mattioli 1885.
AN - rayyan-553781641
AU - Yadav, D.
AU - Kwak, M.
AU - Jin, J. O.
DO - 10.23751/pn.v21i2.7780
IS - 2
KW - Anti-cancerous
Anti-diabetic
Anti-hyperlipidemic
Anti-inflammatory
Diabetes mellitus
Gymnema sylvestre
alpha tocopherol
antidiabetic agent
ascorbic acid
conduritol
cytokine
dihydroxygymnemic triacetate
glimepiride
glucuronide
glutathione
gold nanoparticle
Gymnema sylvestre extract
gymnemagenin
gymnemasaponin V
gymnemate
gymnemic acid
gymnemoside B
hemoglobin A1c
herbaceous agent
insulin
saponin
silver nanoparticle
triterpene
unclassified drug
adjuvant arthritis
antifungal activity
Ayurveda
Candida albicans
developing country
diabetes mellitus
drug safety
glucose blood level
herb
human
hyperlipidemia
hypolipidemic activity
immunomodulation
insulin release
nonhuman
obesity
oral glucose tolerance test
pancreas islet beta cell
phytochemistry
plant leaf
plant stem
Review
toxicity testing
wound healing
PY - 2019
SP - 258-269
ST - Clinical applications of Gymnema sylvestre against type 2 diabetes mellitus
and its associated abnormalities
T2 - Progress in Nutrition
TI - Clinical applications of Gymnema sylvestre against type 2 diabetes mellitus
and its associated abnormalities
85069438506&doi=10.23751%2fpn.v21i2.7780&partnerID=40&md5=dbae2cba521a182c2e05eab5b
219aef1
VL - 21
ID - 9578
ER -
TY - JOUR
AB - Process of wound healing is a complex biological process involving different
overlapping phases to attain reepithelialization. Advancement of nanotechnology in
medical sciences has endowed zinc oxide and iron oxide nanoparticles as novel
therapeutic approaches, which potentially promote the process of wound healing.
Current study was designed to explore the effect of biofabricated zinc oxide and
iron oxide nanoparticles of Prosopis cineraria (PC) leaves extract (ZnPC and FePC,
respectively) on dermal full thickness wounds. In vitro antioxidant activity was
performed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and anti-
inflammatory activity was confirmed by albumin denaturation and proteinase
inhibition method. ZnPC and FePC were synthesized by co-precipitation method via
green route and characterized by various techniques. Various parameters of wound
such as wound closure rate, epithelialization period, hydroxyproline content,
tensile strength and level of antioxidant enzyme as well as inflammatory markers
were evaluated. Different analyses exhibited the spherical particles of 117.5
(ZnPC) and 48 (FePC) nm size. Significant effect (p < 0.05) of nano-ointment
topical formulations on wound contraction rate and epithelialization period was
observed. Hydroxyproline content, inflammatory markers and enzymatic antioxidant
profile also supported the wound healing effect. ZnPC ointment applied group showed
quick healing of tissue injury as compared to FePC ointment applied group.
Antioxidant and anti-inflammatory action, due to the synergistic effect of metal
oxides and polyphenolic compounds of PC leaves, could be the possible underlying
mechanism of swift wound healing property of ZnPC and FePC. Therefore, ZnPC and
FePC based nano-ointment approach could be a beneficial way for clinical treatment
of wounds.
AN - rayyan-553781642
AU - Yadav, E.
AU - Yadav, P.
AU - Verma, A.
DO - 10.1016/j.jddst.2021.102833
KW - Ointments
Rats
Oxalic Acid
Zinc
Rats, Wistar
Norisoprenoids
Iron
PY - 2021
SN - 1773-2247 2588-8943
ST - Amelioration of full thickness dermal wounds by topical application of
biofabricated zinc oxide and iron oxide nano-ointment in albino Wistar rats
TI - Amelioration of full thickness dermal wounds by topical application of
biofabricated zinc oxide and iron oxide nano-ointment in albino Wistar rats
VL - 66
Y2 - 12
ID - 9579
ER -
TY - JOUR
AB - The TRAIL (TNF-related apoptosis-inducing ligand) apoptotic pathway is
extensively exploited in the development of targeted antitumor therapy due to TRAIL
specificity towards its cognate receptors, namely death receptors DR4 and DR5.
Although therapies targeting the TRAIL pathway have encountered many obstacles in
attempts at clinical implementation for cancer treatment, the unique features of
the TRAIL signaling pathway continue to attract the attention of researchers.
Special attention is paid to the design of novel nanoscaled delivery systems,
primarily aimed at increasing the valency of the ligand for improved death receptor
clustering that enhances apoptotic signaling. Optionally, complex nanoformulations
can allow the encapsulation of several therapeutic molecules for a combined
synergistic effect, for example, chemotherapeutic agents or photosensitizers.
Scaffolds for the developed nanodelivery systems are fabricated by a wide range of
conventional clinically approved materials and innovative ones, including metals,
carbon, lipids, polymers, nanogels, protein nanocages, virus-based nanoparticles,
dendrimers, DNA origami nanostructures, and their complex combinations. Most
nanotherapeutics targeting the TRAIL pathway are aimed at tumor therapy and
theranostics. However, given the wide spectrum of action of TRAIL due to its
natural role in immune system homeostasis, other therapeutic areas are also
involved, such as liver fibrosis, rheumatoid arthritis, Alzheimer’s disease, and
inflammatory diseases caused by bacterial infections. This review summarizes the
recent innovative developments in the design of nanodelivery systems modified with
TRAIL pathway-targeting ligands. © 2023 by the authors.
AN - rayyan-553781643
AU - Yagolovich, A. V.
AU - Gasparian, M. E.
AU - Dolgikh, D. A.
IS - 2
KW - death receptors
DR5
drug delivery
ligand-targeted drugs
nanoparticles
nanotherapeutics
receptor clustering
TRAIL
antiinfective agent
caspase 8
CD47 antigen
death receptor
dendrimer
ferritin
folic acid
interleukin 4
nanomaterial
photosensitizing agent
silver nanoparticle
tumor necrosis factor related apoptosis inducing ligand
amino acid sequence
antigen presenting cell
apoptosis
binding affinity
biocompatibility
biodegradability
cancer inhibition
cell surface
cell survival
circulating tumor cell
colony formation
cross linking
DNA damage
drug synthesis
endocytosis
genetic engineering
human
human cell
Klebsiella pneumoniae infection
lactic acid bacterium
liposomal delivery
liver fibrosis
molecular dynamics
nanomedicine
Notch signaling
Review
signal transduction
thermal conductivity
tumor volume
upregulation
PY - 2023
ST - Recent Advances in the Development of Nanodelivery Systems Targeting the
TRAIL Death Receptor Pathway
T2 - Pharmaceutics
TI - Recent Advances in the Development of Nanodelivery Systems Targeting the
TRAIL Death Receptor Pathway
85149115813&doi=10.3390%2fpharmaceutics15020515&partnerID=40&md5=307edb9b0ea82f941d
48e80455c41a16
VL - 15
ID - 9580
ER -
TY - JOUR
AB - Implant therapy can lead to peri-implantitis, and none of the methods used to
treat this inflammatory response have been predictably effective. It is nearly
impossible to treat infected surfaces such as TiUnite (a titanium oxide layer) that
promote osteoinduction, but finding an effective way to do so is essential.
Experiments were conducted to determine the optimum irradiation power for stripping
away the contaminated titanium oxide layer with Er:YAG laser irradiation, the
degree of implant heating as a result of Er:YAG laser irradiation, and whether
osseointegration was possible after Er:YAG laser microexplosions were used to strip
a layer from the surface of implants placed in beagle dogs. The Er:YAG laser was
effective at removing an even layer of titanium oxide, and the use of water spray
limited heating of the irradiated implant, thus protecting the surrounding bone
tissue from heat damage.
AN - rayyan-553782386
AU - Yamamoto, A.
AU - Tanabe, T.
DO - 10.11607/prd.1593
IS - 1
J2 - Int J Periodontics Restorative Dent
KW - Animals
Coated Materials, Biocompatible/*radiation effects
*Dental Implants
Dental Materials/*radiation effects
Dogs
Equipment Contamination/*prevention & control
Female
Hot Temperature
Laser Therapy/*methods
Lasers, Gas/therapeutic use
Lasers, Solid-State/*therapeutic use
Models, Animal
Osseointegration/physiology
Peri-Implantitis/*therapy
Radiotherapy Dosage
Surface Properties
Titanium/*radiation effects
Water
LA - eng
N1 - The Japan Institute for Advanced Dental Studies, Osaka and Tokyo, Japan.
autis@silver.ocn.ne.jp
PY - 2013
SP - 21-30
ST - Treatment of peri-implantitis around TiUnite-surface implants using Er:YAG
laser microexplosions
T2 - The International journal of periodontics & restorative dentistry
TI - Treatment of peri-implantitis around TiUnite-surface implants using Er:YAG
laser microexplosions
VL - 33
Y2 - 1
ID - 10293
ER -
TY - JOUR
AB - A green synthesis of silver nanoparticles (AgNPs) using aqueous Lonicera
japonica leaf extract (AgNPs-LLJ) was reported and their anti-inflammatory and
antibacterial effects were investigated. In comparison, AgNPs were also synthesised
using the liquid phase chemical reduction method (AgNPs-N2H4H2O). The synthesised
AgNPs were identified using multiple analytical techniques. The results showed that
both AgNPs were spherical particles with an average particle size of 20-30 nm for
AgNPs-LLJ and 10-20 nm for AgNPs-N2H4H2O. The anti-inflammatory ability of AgNPs
was shown by the effective inhibition against 5-lipoxygenase with IC50 values of
5.08 μg/mL for AgNPs-LLJ and 59.12 μg/mL for AgNPs-N2H4H2O, indicating that AgNPs-
LLJ had much more obvious antiinflammatory effect. Furthermore, the cytotoxicity
research on RAW264.7 mouse macrophages showed that AgNPs-LLJ had no cytotoxicity at
the concentration of 40 μg/mL compared with control. Their antibacterial effects on
Escherichia coli (EC) and Staphlococcus aureus (SA) were studied by agar well
diffusion method. The maximum antibacterial circles of AgNPs-N2H4H2O on EC and SA
were 15 and 14 mm, respectively, whereas it was 5.0 and 5.0 mm for AgNPs-LLJ,
respectively. The authors conclude that AgNPs-LLJs have a potential to be a
nanomedicine for nanobiomedical applications. © The Institution of Engineering and
Technology 2019.
AN - rayyan-553781646
AU - Yan, L.
AU - Qiu, B.
AU - Li, T.
AU - Wu, D.
AU - Zhu, J.
AU - Zhao, D.
DO - 10.1049/mnl.2019.0343
IS - 2
KW - Escherichia coli
Metal nanoparticles
Particle size
gentamicin
Lonicera japonica extract
nordihydroguaiaretic acid
silver nanoparticle
Anti-inflammatories
Anti-inflammatory effects
Antibacterial effects
Average particle size
Chemical reduction methods
Diffusion method
Spherical particle
animal cell
Article
bacteriostatic activity
controlled study
drug cytotoxicity
drug synthesis
enzyme activity
enzyme inhibition
green chemistry
IC50
in vitro study
Lonicera japonica
macrophage
mouse
nanomedicine
nonhuman
particle size
plant leaf
RAW 264.7 cell line
zeta potential
Silver compounds
PY - 2020
SP - 90-95
ST - Green synthesis of silver nanoparticles from Lonicera japonica leaf extract
and their anti-inflammatory and antibacterial effects
T2 - Micro and Nano Letters
TI - Green synthesis of silver nanoparticles from Lonicera japonica leaf extract
and their anti-inflammatory and antibacterial effects
85078860427&doi=10.1049%2fmnl.2019.0343&partnerID=40&md5=21539cadfc3374ad555b56ea15
0e4c19
VL - 15
ID - 9583
ER -
TY - JOUR
AB - Chronic sclerosing unspecific sialadenitis or KYttner tumor, is an infrequent
inflammatory lesion of submandibular gland. We report a 60 years old male
presenting with a slowly growing, painless, bilateral submandibular tumor of two
years of evolution. Pathological examinations showed marked atrophy of glandular
parenchyma with increased fibrous connective tissue and an intense lymphocytic
infiltration with lymphoid follicle formation. Lymphocyte population study with
kappa, lambda, CD20 and CD45RO antibodies was similar to that observed in reactive
lymph nodes. There was no over expression of Bcl-2 gene protein, involved in the
phenomenon of apoptosis of glandular tissue, that could explain the pathogenesis of
atrophy. This protein was positive only in lymphoid cells and glandular conducts.
An immune etiology, with replacement of glandular tissue by lymphoid and fibrous
connective tissue is suggested
AN - rayyan-553781647
AU - Yáñez M, Milly
AU - Roa Esterio, Iván
AU - Roa S, Juan Carlos
AU - Villaseca H, Miguel Angel
AU - García V, Marcela
IS - 5
KW - Sialadenitis
LA - es
PY - 1999
SN - 0034-9887
SP - 600-3
ST - Sialadenitis crónica esclerosante inespecífica de la glándula submandibular,
tumor de Kuttner: caso clínico
T2 - Rev. méd. Chile
TI - Sialadenitis crónica esclerosante inespecífica de la glándula submandibular,
tumor de Kuttner: caso clínico
UR - https://pesquisa.bvsalud.org/portal/resource/pt/lil-243935
VL - 127
Y2 - 5 y3 - 1
ID - 9584
ER -
TY - JOUR
AB - Purpose: Pt-based nanostructures are one of the promising nanomaterials for
being used in catalysts, sensors, and therapeutics. However, their impacts on the
health and biological systems are not adequately understood yet.Methods: In this
work, nanorods composed of ultrasmall platinum (Pt) nanoparticles deposited on the
surface and gold nanorod as the core (Au@Pt NRs) were synthesized, and the
distribution and toxic effects of Au@Pt NRs were investigated in C57BL/6 mice with
intravenous injection by using atomic absorption spectroscopy (AAS), transmission
electron microscope (TEM), hematoxylin-eosin (HE) staining and blood cell
analyzer.Results: At the time point of Day 1, Day 8 and Day 16 post injection of
Au@Pt NRs (6 mg/kg of Pt atom), Au@Pt NRs were mainly accumulated in the liver and
spleen. The energy dispersive spectrometer mapping images showed Au@Pt NRs
experienced quick corrosion and Au released faster than Pt in the physiological
environments. The catalase (CAT) activity in tissues increased slightly in the
early stage of the Au@Pt NRs exposure and went down to the normal level. With HE
staining, inflammatory cells infiltration could be seen in the tissues, while no
significant influences were detected on the blood biochemistry and the function of
liver and kidney.Conclusion: In conclusion, intravenously injected Au@Pt NRs mainly
distributed in the liver and spleen with comparable levels, and did not exert any
significant toxic effects on the organs' function within two weeks; meanwhile,
Au@Pt NRs were able to degrade, which indicated acceptable safety to the mice and
potentials of biomedical application.
AN - rayyan-553781648
AU - Yang, A. Y.
AU - Wen, T.
AU - Hao, B. Y.
AU - Meng, Y. L.
AU - Zhang, X.
AU - Wang, T.
AU - Meng, J.
AU - Liu, J.
AU - Wang, J. H.
AU - Xu, H. Y.
DO - 10.2147/IJN.S386476
KW - Mice
PY - 2022
SN - 1178-2013
SP - 5339-5351
ST - Biodistribution and Toxicological Effects of Ultra-Small Pt Nanoparticles
Deposited on Au Nanorods (Au@Pt NRs) in Mice with Intravenous Injection
TI - Biodistribution and Toxicological Effects of Ultra-Small Pt Nanoparticles
Deposited on Au Nanorods (Au@Pt NRs) in Mice with Intravenous Injection
VL - 17
ID - 9585
ER -
TY - JOUR
AB - Polyphenols are micronutrients that are widely present in human daily diets.
Numerous studies have demonstrated their potential as antioxidants and anti-
inflammatory agents, and for cancer prevention, heart protection and the treatment
of neurodegenerative diseases. However, due to their vulnerability to environmental
conditions and low bioavailability, their application in the food and medical
fields is greatly limited. Nanoformulations, as excellent drug delivery systems,
can overcome these limitations and maximize the pharmacological effects of
polyphenols. In this review, we summarize the biological activities of polyphenols,
together with systems for their delivery, including phospholipid complexes, lipid-
based nanoparticles, protein-based nanoparticles, niosomes, polymers, micelles,
emulsions and metal nanoparticles. The application of polyphenol nanoparticles in
food and medicine is also discussed. Although loading into nanoparticles solves the
main limitation to application of polyphenolic compounds, there are some concerns
about their toxicological safety after entry into the human body. It is therefore
necessary to conduct toxicity studies and residue analysis on the carrier.
AN - rayyan-553781649
AU - Yang, B. Y.
AU - Dong, Y. X.
AU - Wang, F.
AU - Zhang, Y.
DO - 10.3390/molecules25204613
IS - 20
KW - Biological Availability
Polyphenols
PY - 2020
SN - 1420-3049
ST - Nanoformulations to Enhance the Bioavailability and Physiological Functions
of Polyphenols
T2 - MOLECULES
TI - Nanoformulations to Enhance the Bioavailability and Physiological Functions
of Polyphenols
VL - 25
Y2 - 10
ID - 9586
ER -
TY - JOUR
AB - Multiple sclerosis (MS) is a chronic inflammatory disease of the central
nervous system (CNS) in which the immune system damages the protective insulation
surrounding nerve fibers that project from neurons. The pathological hallmark of MS
is multiple areas of myelin loss accompanied by inflammation within the CNS,
resulting in loss of cognitive function that ultimately leads to paralysis. Recent
studies in MS have focused on autophagy, a cellular self-eating process, as a
potential target for MS treatment. Here, we review the contribution of immune cell
autophagy to the pathogenesis of experimental autoimmune encephalomyelitis (EAE),
the prototypic animal model of MS. A better understanding of the role of autophagy
in different immune cells to EAE might inform the development of novel therapeutic
approaches in MS and other autoimmune and inflammatory diseases. © Copyright © 2021
Yang and Van Kaer.
AN - rayyan-553781654
AU - Yang, G.
AU - Van Kaer, L.
DO - 10.3389/fimmu.2021.724108
KW - autophagy
experimental autoimmune encephalomyelitis (EAE)
immune cells
LC3-associated phagocytosis (LAP)
multiple sclerosis
therapy
Adaptive Immunity
Animals
Autophagy
Encephalomyelitis, Autoimmune, Experimental
Humans
Immunity, Innate
Multiple Sclerosis
T-Lymphocytes
3 methyladenine
aspartic acid
autoantigen
autophagy related protein 5
autophagy related protein 7
caspase recruitment domain protein 15
chloroquine
estrogen receptor
fk 506 binding protein
interleukin 1beta
mammalian target of rapamycin
myelin
myelin oligodendrocyte glycoprotein
nicotinic receptor
pertussis toxin
procaspase 8
sequestosome 1
tamoxifen
apoptosis
axon
blood brain barrier
bone marrow cell
CD4+ T lymphocyte
cell infiltration
cognition
cytokine production
cytokinesis
cytoplasm
dendritic cell
endocytosis
endoplasmic reticulum
experimental autoimmune encephalomyelitis
Golgi complex
immune system
immunization
intravital microscopy
lymphocyte
macrophage
melanogenesis
microglia
myelin sheath
nerve cell
nerve fiber
neutrophil
nonhuman
oligodendroglia
paralysis
pathogenesis
pathophysiology
peripheral lymphocyte
phagocytosis
phenotype
Review
single cell RNA seq
spinal cord
systematic review
T lymphocyte
Th1 cell
Th17 cell
thymocyte
adaptive immunity
animal
disease model
human
immunology
innate immunity
PY - 2021
ST - Therapeutic Targeting of Immune Cell Autophagy in Multiple Sclerosis: Russian
Roulette or Silver Bullet?
TI - Therapeutic Targeting of Immune Cell Autophagy in Multiple Sclerosis: Russian
Roulette or Silver Bullet?
85114778006&doi=10.3389%2ffimmu.2021.724108&partnerID=40&md5=d193b97108faafee9c7ffd
b84a206aec
VL - 12
ID - 9591
ER -
TY - JOUR
AB - Titanium and Titanium alloys are widely used as biomedical implants in oral
and maxillofacial surgery, due to superior mechanical properties and
biocompatibility. In specific clinical populations such as the elderly, diabetics
and patients with metabolic diseases, the failure rate of medical metal implants is
increased significantly, putting them at increased risk of revision surgery. Many
studies show that the content of reactive oxygen species (ROS) in the
microenvironment of bone tissue surrounding implant materials is increased in
patients undergoing revision surgery. In addition, the size and shape of materials,
the morphology, wettability, mechanical properties, and other properties play
significant roles in the production of ROS. The accumulated ROS break the original
balance of oxidation and anti-oxidation, resulting in host oxidative stress. It may
accelerate implant degradation mainly by activating inflammatory cells. Peri-
implantitis usually leads to a loss of bone mass around the implant, which tends to
affect the long-term stability and longevity of implant. Therefore, a great deal of
research is urgently needed to focus on developing antibacterial technologies. The
addition of active elements to biomedical titanium and titanium alloys greatly
reduce the risk of postoperative infection in patients. Besides, innovative
technologies are developing new biomaterials surfaces conferring anti-infective
properties that rely on the production of ROS. It can be considered that ROS may
act as a messenger substance for the communication between the host and the
implanted material, which run through the entire wound repair process and play a
role that cannot be ignored. It is necessary to understand the interaction between
oxidative stress and materials, the effects of oxidative stress products on
osseointegration and implant life as well as ROS-induced bactericidal activity.
This helps to facilitate the development of a new generation of well-biocompatible
implant materials with ROS responsiveness, and ultimately prolong the lifespan of
implants.
AN - rayyan-553781655
AU - Yang, J.
AU - Liu, C.
AU - Sun, H.
AU - Liu, Y.
AU - Liu, Z. G.
AU - Zhang, D.
AU - Zhao, G.
AU - Wang, Q.
AU - Yang, D. H.
DO - 10.3389/fbioe.2022.1092916
KW - Titanium
Oxygenators
PY - 2022
SN - 2296-4185
ST - The progress in titanium alloys used as biomedical implants: From the view of
TI - The progress in titanium alloys used as biomedical implants: From the view of
VL - 10
Y2 - 12 y3 - 19
ID - 9592
ER -
TY - JOUR
AB - A novel antibacterial strategy is urgently required to develop for solving
bacterial biofilm obstruction and bacterial drug resistance in the infected wound
healing process. Herein, the Chitosan/Bletilla striata polysaccharide composited
microneedles were prepared by chitosan, tannic acid, AgNO3 and Bletilla striata
polysaccharide through step centrifugation. In our design system, the porous
structure of microneedles gradually disappeared, and the mechanical properties were
significantly improved after multiple fillings. Ag+ is reduced in-situ to silver
nanoparticles by the abundant polyphenols of tannic acid, displaying antibacterial
effects both in vitro and vivo, even for methicillin resistant Staphylococcus
aureus. The addition of Bletilla striata polysaccharide increased the ability of
piercing biofilm and promoted wound healing. The microneedles exhibited good
biocompatibility and with function of piercing the bacterial biofilms, scavenging
excessive free radicals, inhibiting inflammatory factors, and promoting wound
healing. Therefore, the multifunctional composited microneedles show great
potential to promote infected and susceptible wound healing. © 2022
AN - rayyan-553781657
AU - Yang, X.
AU - Jia, M.
AU - Li, Z.
AU - Ma, Z.
AU - Lv, J.
AU - Jia, D.
AU - He, D.
AU - Zeng, R.
AU - Luo, G.
AU - Yu, Y.
DO - 10.1016/j.ijbiomac.2022.06.131
KW - Bacterial infection
Bletilla striata polysaccharide
Chitosan
In-situ synthesis
Microneedle
Wound healing
Metal Nanoparticles
Orchidaceae
Polysaccharides
Silver
Tannins
Wound Healing
Bacteria
Biofilms
Drug delivery
Flavonoids
Free radicals
Metal nanoparticles
Needles
Silver compounds
chitosan
dimeticone
interleukin 10
polyphenol
polysaccharide
silver nanoparticle
silver nitrate
tannin
trichloroethane
vasculotropin
antiinfective agent
metal nanoparticle
silver
tannin derivative
Antibacterials
Bacterial biofilm
Bletillum striatum polysaccharide
Composited
Microneedles
Striatum
Tannic acid
animal cell
animal experiment
animal model
apoptosis
Article
bacterial infection
biocompatibility
biofilm
Bletilla
cell proliferation
controlled study
cytotoxicity
Escherichia coli
in vitro study
MTT assay
nonhuman
oxidative stress
photoelectron spectroscopy
rat
tensile strength
wound healing
X ray diffraction
chemistry
PY - 2022
SP - 550-559
ST - In-situ synthesis silver nanoparticles in chitosan/Bletilla striata
polysaccharide composited microneedles for infected and susceptible wound healing
TI - In-situ synthesis silver nanoparticles in chitosan/Bletilla striata
polysaccharide composited microneedles for infected and susceptible wound healing
85135500358&doi=10.1016%2fj.ijbiomac.2022.06.131&partnerID=40&md5=7f7f4857188b0158b
a4b32ea29663ea9
VL - 215
ID - 9594
ER -
TY - JOUR
AB - The present study was to investigate the effects of visfatin on the
morphological structure and function of the rat uterus during inflammation. The
expression and distribution of visfatin, morphological structure, eosinophils
(EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat
were studied using hematoxylin-eosin staining (HE), immunohistochemical methods,
western blots and enzyme-linked immunosorbent assay (ELISA). The present study
showed that visfatin positive cells dispersed widely in the uterus, and strong
positive staining was observed mainly in the cell cytoplasm. Compared with saline
group, in visfatin group, more uterine glands were found, EOS increased, and the
difference was significant (P<0.05), MPO reduced, and the difference was
significant (P<0.01). In addition, visfatin was able to increase the secretion of
IL-1b, IL-6, and TNF-a (P<0.01). Compared with LPS group, in vifatin+LPS group, the
uterine glands of the lamina propria increased, the myometrium became thinner, the
number of EOS and MPO reduced obviously, but the difference was not significant
(P>0.05), and after LPS stimulated body, visfatin decrease the level of IL-1b, IL-
6, TNF-a (P<0.01). The above results suggest that visfatin could affect the
morphological structure of rat uterus; Visfatin could modulate the inflammatory
response in rats' uterus by regulating the quantity of inflammatory cells, such as
EOS and MPO, and the level of inflammatory cytokines, such as IL-1b, IL-6, TNF-a.
El objetivo del presente estudio fue investigar los efectos de la visfatina sobre
la estructura morfológica y la función del útero de la rata durante la inflamación.
Se estudiaron la expresión y distribución de la visfatina, la estructura
morfológica, eosinófilos, mieloperoxidasa y citoquinas en el útero de rata mediante
la tinción de H&E, métodos inmunohistoquímicos, Western blots y ELISA. El estudio
mostró que las células visfatina positivas se dispersan ampliamente en el útero,
junto a una fuerte tinción positiva, principalmente en el citoplasma de la célula.
En comparación con el grupo control, en el grupo visfatina, se encontraron más
glándulas uterinas, se observó un aumento de EOS y la diferencia fue significativa
(p<0,05), MPO reducida siendo esta diferencia también significativa (p<0,01).
Además, la visfatina fue capaz de aumentar la secreción de IL-1b, IL-6 y TNF-a
(P<0,01). En comparación con el grupo LPS, visfatina+grupo LPS, las glándulas
uterinas de la lámina propia aumentaron, se observó un miometrio más delgado, y
número reducido de EOS y MPO, sin embargo, la diferencia no fue significativa
(P>0,05). Después de estímulo LPS en el cuerpo, se registró un nivel menor de
visfatina en IL-1b, IL-6, TNF-a (P<0,01). Los resultados anteriores sugieren que
visfatina podría afectar a la estructura morfológica del útero de rata. Además,
podría modular la respuesta inflamatoria en el útero mediante la regulación de la
cantidad de células inflamatorias, tales como EOS y MPO.
AN - rayyan-553781660
AU - Yang, Zhi
AU - Xiao, Ke
AU - Wang, Wei
AU - Tang, Juan
AU - Sun, Peng-Peng
AU - Peng, Ke-Mei
AU - Song, Hui
DO - 10.4067/S0717-95022015000100031
IS - 1
KW - Citoquinas
Cytokine
Inflamación
Inflammation
LPS-induced rat
Rata inducida con lipopolisacáricos
Utero
Uterus
Visfatin
Visfatina
Rats
LA - en
PY - 2015
SN - 0717-9367
SP - 194-203
ST - The effect of visfatin on inflammatory reaction in uterus of LPS-induced rats
TI - The effect of visfatin on inflammatory reaction in uterus of LPS-induced rats
95022015000100031
VL - 33
Y2 - 3 y3 - 1
ID - 9597
ER -
TY - JOUR
AB - Titanium (Ti) with nanoscale structure on the surface exhibits excellent
biocompatibility and bone integration. Once implanted, the surgical implantation
may lead to bacterial infection and inflammatory reaction, which cause the implant
failure. In this work, irregular and nanorod-shaped ZnO nanoparticles were doped
into TiO2 nanotubes (TNTs) with inner diameter of about 50 nm by electro-
deposition. The antibacterial properties of ZnO incorporated into TiO2 nanotubes
(TNTs/ZnO) were evaluated using Staphylococcus aureus (S. aureus). Zn ions released
from the nanoparticles and the morphology could work together, improving
antibacterial effectiveness up to 99.3% compared with the TNTs. Macrophages were
cultured on the samples to determine their respective anti-inflammatory properties.
The proliferation and viability of macrophages were evaluated by the CCK-8 method
and Live&Dead stain, and the morphology of the cells was observed by scanning
electron microscopy. Results indicated that TNTs/ZnO has a significant inhibitory
effect on the proliferation and adhesion of macrophages, which could be used to
prevent chronic inflammation and control the inflammatory reaction. Besides, the
release of Zn ions from the ZnO nanoparticles is a long-term process, which could
be beneficial for bone integration. Results demonstrate that ZnO deposited into
TNTs improved the antibacterial effectiveness and weakened the inflammatory
reaction of titanium-based implants, which is a promising approach to enhance their
bioactivity.
AN - rayyan-553781661
AU - Yao, S. L.
AU - Feng, X. J.
AU - Lu, J. J.
AU - Zheng, Y. D.
AU - Wang, X. M.
AU - Volinsky, A. A.
AU - Wang, L. N.
DO - 10.1088/1361-6528/aabac1
IS - 24
KW - Inflammation
PY - 2018
SN - 0957-4484 1361-6528
ST - Antibacterial activity and inflammation inhibition of ZnO nanoparticles
embedded TiO2 nanotubes
T2 - NANOTECHNOLOGY
TI - Antibacterial activity and inflammation inhibition of ZnO nanoparticles
embedded TiO2 nanotubes
VL - 29
Y2 - 6 y3 - 15
ID - 9598
ER -
TY - JOUR
AB - Metallic nanoparticles (MNPs) are new engineering materials with broad
prospects for biomedical applications; thus, their biosafety has drawn great
concern. The liver is the main detoxification organ of vertebrates. However, many
issues concerning the interactions between MNPs and biological systems (cells and
tissues) are unclear, particularly the toxic effects of MNPs on hepatocytes and
other liver cells. Numerous researchers have shown that some MNPs can induce
decreased cell survival rate, production of reactive oxygen species (ROS),
mitochondrial damage, DNA strand breaks, and even autophagy, pyroptosis, apoptosis,
or other forms of cell death. Our review focuses on the recent researches on the
liver toxicity of MNPs and its mechanisms at cellular and subcellular levels to
provide a scientific basis for the subsequent hepatotoxicity studies of MNPs.
AN - rayyan-553781662
AU - Yao, Y.
AU - Zang, Y. T.
AU - Qu, J.
AU - Tang, M.
AU - Zhang, T.
DO - 10.2147/IJN.S212907
KW - Liver
PY - 2019
SN - 1178-2013
SP - 8787-8804
ST - The Toxicity Of Metallic Nanoparticles On Liver: The Subcellular Damages,
Mechanisms, And Outcomes
TI - The Toxicity Of Metallic Nanoparticles On Liver: The Subcellular Damages,
Mechanisms, And Outcomes
VL - 14
ID - 9599
ER -
TY - JOUR
AB - OBJECTIVE: To see the toxic effects of NSAID on kidney tissue of albino rats.
METHODS: For this experimental study, 16 albino rats were taken. They were divided
into two groups; A and B. The animals in group-A were given Normal Saline. Group-B
received Diclofenac Sodium 2 mg/kg/day by feeding tube for 14 days. On day-15 all
animals were sacrificed. Kidneys were removed, fixed, embedded in paraffin, section
cut at 4 microm thick and stained with H&E, PAS, and silver methenamine. Renal
histology was done under light microscope to see the renal tubular diameter, count
and cellular detail. RESULTS: The result of present study revealed that diclofenac
sodium in single daily dose of 2 mg/kg for a period of two weeks effectively
produced destruction of proximal and distal convoluted tubules in adult albino rats
showing the dilatation of tubule and flattening of tubular epithelium, disruption
of brush border in proximal tubule and thickening of basement membrane around
proximal and distal tubular epithelium. CONCLUSION: Diclofenac sodium induced
nephrotoxicity causes the destruction of proximal and distal convoluted tubules
showing the dilatation of tubule and flattening of its epithelium in albino rats.
AN - rayyan-553782219
AU - Yasmeen, T.
AU - Qureshi, G. S.
AU - Perveen, S.
IS - 7
J2 - J Pak Med Assoc
KW - Animals
Anti-Inflammatory Agents, Non-Steroidal/*adverse effects
Diclofenac/*adverse effects
Epithelium/*drug effects
Kidney/*drug effects
Kidney Tubules, Distal/drug effects
Kidney Tubules, Proximal/drug effects
Models, Animal
Rats
Diclofenac
LA - eng
N1 - Department of Anatomy, Sindh Medical College, Jinnah Postgraduate Medical
Centre, Karachi.
PY - 2007
SN - 0030-9982 (Print)
SP - 349-51
ST - Adverse effects of diclofenac sodium on renal parenchyma of adult albino rats
T2 - JPMA. The Journal of the Pakistan Medical Association
TI - Adverse effects of diclofenac sodium on renal parenchyma of adult albino rats
VL - 57
Y2 - 7
ID - 10129
ER -
TY - JOUR
AB - ICR mice were injected with ketamine for 1, 3 and 6 months and the kidneys
and urinary bladders were excised and processed for histology. Starting from 1
month, all addicted mice showed invasion of mononuclear white cells, either
surrounding the glomerulus or the other tubules in the kidney. The aggregation of
these cells extended all the way to the pelvis and ureter. As well, in the urinary
bladder, the epithelium became thin and there was submucosal infiltration of
mononuclear inflammatory cells. Silver staining revealed a loss of nerve fibers
amongst the muscles of the urinary bladder of the treated. Immunohistochemistry on
choline acetyltransferase which is a marker for cholinergic neurons also
demonstrated a decrease of those cells. We hypothesized that prolonged ketamine
addiction resulted in the animals prone to urinary infection.
AN - rayyan-553782173
AU - Yeung, L. Y.
AU - Rudd, J. A.
AU - Lam, W. P.
AU - Mak, Y. T.
AU - Yew, D. T.
DO - 10.1016/j.toxlet.2009.09.006
IS - 2
J2 - Toxicol Lett
KW - Anesthetics, Dissociative/pharmacokinetics/*toxicity
Animals
Choline O-Acetyltransferase/metabolism
Disease Progression
Ketamine/pharmacokinetics/*toxicity
Kidney/pathology
Kidney Diseases/*chemically induced/*pathology
Male
Mice
Mice, Inbred ICR
Muscle, Smooth/pathology
Nerve Fibers/pathology
Silver Staining
Substance-Related Disorders/*pathology
Urinary Bladder/pathology
Kidney
Ketamine
LA - eng
N1 - School of Biomedical Sciences, Faculty of Medicine, Chinese University of
Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
PY - 2009
SP - 275-8
ST - Mice are prone to kidney pathology after prolonged ketamine addiction
T2 - Toxicology letters
TI - Mice are prone to kidney pathology after prolonged ketamine addiction
VL - 191
Y2 - 12 y3 - 15
ID - 10084
ER -
TY - JOUR
AB - Chlamydia trachomatis is a very common sexually transmissible infection in
both developing and developed countries. A hallmark of C. trachomatis infection is
the induction of severe inflammatory responses which play critical roles in its
pathogenesis. Antibiotics are the only treatment option currently available for
controlling C. trachomatis infection; however, they are efficacious only when
administered early after an infection. The objectives of this study are to explore
alternative strategies in the control and regulation of inflammatory responses
triggered by a C. trachomatis infection. We employed silver-polyvinyl pyrrolidone
(Ag-PVP) nanoparticles, which have been shown to possess anti-inflammatory
properties, as our target and the in vitro mouse J774 macrophage model of C.
trachomatis infection. Our hypothesis is that small sizes of Ag-PVP nanoparticles
will control inflammatory mediators triggered by a C. trachomatis infection.
Cytotoxicity studies using Ag-PVP nanoparticles of 10, 20, and 80 nm sizes revealed
>80% macrophage viability up to a concentration of 6.25 μg/mL, with the 10 nm size
being the least toxic. All sizes of Ag-PVP nanoparticles, especially the 10 nm
size, reduced the levels of the prototypic cytokines, tumor necrosis factor (TNF)
and interleukin (IL)-6, as elicited from C. trachomatis infected macrophages.
Additionally, Ag-PVP nanoparticles (10 nm) selectively inhibited a broad spectrum
of other cytokines and chemokines produced by infected macrophages. Of
significance, Ag-PVP nanoparticles (10 nm) caused perturbations in a variety of
upstream (toll like receptor 2 [TLR2], nucleotide-binding oligomerization-protein 2
[NOD2], cluster of differentiation [CD]40, CD80, and CD86) and downstream (IL-1
receptor-associated kinase 3 [IRAK3] and matrix metallopeptidase 9 [MMP9])
inflammatory signaling pathways by downregulating their messenger ribonucleic acid
(mRNA) gene transcript expressions as induced by C. trachomatis in macrophages.
Collectively, our data provides further evidence for the anti-inflammatory
properties of Ag-PVP nanoparticles, and opens new possibilities for smaller sizes
of Ag-PVP nanoparticles to be employed as regulators of inflammatory responses
induced by C. trachomatis.
AN - rayyan-553781860
AU - Yilma, A. N.
AU - Singh, S. R.
AU - Dixit, S.
AU - Dennis, V. A.
DO - 10.2147/IJN.S44090
KW - Animals
Anti-Inflammatory Agents/chemistry/*pharmacology
B7 Antigens/analysis/metabolism
Cell Line
*Chlamydia trachomatis
Cytokines/analysis/metabolism
Host-Pathogen Interactions/*drug effects
*Macrophages/drug effects/metabolism/microbiology
Mice
Povidone/chemistry/*pharmacology
Silver
Polyvinyls
Macrophages
LA - eng
N1 - Center for Nanobiotechnology and Life Sciences Research, Alabama State
University, Montgomery, AL 36104, USA.
PY - 2013
SP - 2421-32
ST - Anti-inflammatory effects of silver-polyvinyl pyrrolidone (Ag-PVP)
nanoparticles in mouse macrophages infected with live Chlamydia trachomatis
TI - Anti-inflammatory effects of silver-polyvinyl pyrrolidone (Ag-PVP)
nanoparticles in mouse macrophages infected with live Chlamydia trachomatis
VL - 8
ID - 9785
ER -
TY - JOUR
AB - Background: Xixin has been widely used as a traditional Chinese medicine for
headache, toothache and inflammatory diseases. Clinical investigation indicated
that adverse drug reactions occurred with an overdose of xixin, but the toxic
mechanism of xixin in vivo based on trace elements has not been researched yet.
Objective: To explore the in vivo toxic mechanism of xixin induced by trace
elements. Materials and Methods: The contents of trace elements in the serum and
liver of mice were determined by inductively coupled plasma-mass spectrometry (ICP-
MS) after obtaining xixin extracts. Principal component analysis (PCA) and cluster
analysis (CA) were performed between the trace elements' content and dosage using
the software GeneSpring 12.1 to analyze the main toxic elements in vivo. Results:
Trace elements' contents were obviously raised after xixin extracts were taken as a
dosage of 150 mg/mL and 50 mg/mL, respectively. Na, Ca, Cu and Cd in serum and Ca
and Zn in liver were the main trace elements inducing the toxic reaction of xixin.
Conclusion: Xixin possesses the potential function of indirectly upregulating trace
elements in vivo. This study, for the first time, elucidated the in vivo toxic
mechanism of xixin based on trace elements. This method could also be utilized in
the research of corresponding aspects.
AN - rayyan-553781670
AU - Yong-Rui, B.
AU - Xin-Xin, Y.
AU - Shuai, W.
AU - Xian-Sheng, M.
AU - Rui-Qing, Z.
AU - Yue-Ming, X.
AU - Lin, C.
DO - 10.4103/0973-1296.131025
IS - 38
KW - Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag
cluster analysis
inductively coupled plasma-mass spectrometry
principal component analysis
toxicity
aluminum
arsenic
beryllium
cadmium
calcium
Chinese drug
chromium
copper
iron
magnesium
manganese
potassium
selenium
silver
sodium
trace element
unclassified drug
vanadium
xixin
zinc
herbaceous agent
plant extract
xixin extract
adult
animal experiment
article
drug blood level
drug liver level
drug mechanism
female
flow rate
in vivo study
limit of detection
mass spectrometry
metabolism
mouse
nonhuman
peristaltic pump
animal model
animal tissue
Article
blood level
Chinese medicine
controlled study
data analysis software
liver level
Trace Elements
Mass Spectrometry
PY - 2014
SP - 141-146
ST - Study on the in vivo toxic mechanism of xixin based on trace elements
determination by inductively coupled plasma-mass spectrometry
T2 - Pharmacognosy Magazine
TI - Study on the in vivo toxic mechanism of xixin based on trace elements
determination by inductively coupled plasma-mass spectrometry
84899432295&doi=10.4103%2f0973-
1296.131025&partnerID=40&md5=731be72204c783043ad5fffcf632031d
VL - 10
ID - 9606
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) have well-known antimicrobial properties and
have been widely applied as nano-strategies in myriad medical fields. Indeed, early
growth response (Egr-1) is an important transcription factor that has been
implicated in numerous inflammatory diseases. The administration of Ag-NPs
transiently increased the levels of Egr-1 mRNA in human keratinocytes with maximal
induction at the 30 minute time point. In an effort to identify molecular
signatures associated with cellular responses to Ag-NPs, we adopted an approach
involving gene expression profiling of human keratinocytes treated with or without
Egr-1 siRNA transfection using a cDNA microarray. The microarray analysis
demonstrated that: the expression profile of the genes involved in inflammation and
immune response were either stimulated or repressed. Overall, this study was
sufficient to reliably recognize the engagement of Egr-1-driven molecular signaling
pathways in Ag-NPs-treated keratinocytes.
AN - rayyan-553781671
AU - Yoo, G.
AU - Jeong, S. H.
AU - Ryu, W. I.
AU - Lee, H.
AU - Kim, J. H.
AU - Bae, H. C.
AU - Son, S. W.
DO - 10.1007/s13273-014-0016-9
IS - 2
KW - Humanities
Humanism
Humans
Gene Expression
Keratinocytes
PY - 2014
SN - 1738-642X 2092-8467
SP - 149-156
ST - Gene expression analysis reveals a functional role for the Ag-NPs-induced
Egr-1 transcriptional factor in human keratinocytes
T2 - MOLECULAR & CELLULAR TOXICOLOGY
TI - Gene expression analysis reveals a functional role for the Ag-NPs-induced
Egr-1 transcriptional factor in human keratinocytes
VL - 10
Y2 - 6 y3 - 30
ID - 9607
ER -
TY - JOUR
AB - Ganoderma lucidum is a traditional Oriental medicine that has been widely
used as a tonic to promote longevity and health in Korea and other Asian countries.
Although a great deal of work has been carried out on the therapeutic potential of
this mushroom, the pharmacological mechanisms of its anti-inflammatory actions
remain unclear. In this study, we evaluated the inhibitory effects of G. lucidum
ethanol extract (EGL) on the production of inflammatory mediators and cytokines in
lipopolysaccharide (LPS)-stimulated murine BV2 microglia. We also investigated the
effects of EGL on the LPS-induced activation of nuclear factor kappaB (NF-kappa B)
and upregulation of toll-like receptor 4 (TLR4) and myeloid differentiation factor
88 (MyD88). Elevated levels of nitric oxide (NO), prostaglandin E-2 (PGE(2)) and
pro-inflammatory cytokine production were detected in BV2 microglia following LPS
stimulation. We identifed that EGL significantly inhibits the excessive production
of NO, PGE(2) and pro-inflammatory cytokines, including interleukin (IL)-1 beta and
tumor necrosis factor-alpha in a concentration-dependent manner without causing
cytotoxicity. In addition, EGL suppressed NF-kappa B translocation and
transcriptional activity by blocking I kappa B degradation and inhibiting TLR4 and
MyD88 expression in LPS-stimulated BV2 cells. Our results indicate that the
inhibitory effects of EGL on LPS-stimulated inflammatory responses in BV2 microglia
are associated with the suppression of the NF-kappa B and TLR signaling pathways.
Therefore, EGL may be useful in the treatment of neurodegenerative diseases by
inhibiting inflammatory mediator responses in activated microglia.
AN - rayyan-553781672
AU - Yoon, H. M.
AU - Jang, K. J.
AU - Han, M. S.
AU - Jeong, J. W.
AU - Kim, G. Y.
AU - Lee, J. H.
AU - Choi, Y. H.
DO - 10.3892/etm.2013.895
IS - 3
KW - Toll-Like Receptors
Ethanol
Ganoderma
Reishi
PY - 2013
SN - 1792-0981 1792-1015
SP - 957-963
ST - Ganoderma lucidum ethanol extract inhibits the inflammatory response by
suppressing the NF-kappa B and toll-like receptor pathways in lipopolysaccharide-
stimulated BV2 microglial cells
T2 - EXPERIMENTAL AND THERAPEUTIC MEDICINE
TI - Ganoderma lucidum ethanol extract inhibits the inflammatory response by
suppressing the NF-kappa B and toll-like receptor pathways in lipopolysaccharide-
stimulated BV2 microglial cells
VL - 5
Y2 - 3
ID - 9608
ER -
TY - JOUR
AB - BACKGROUND: Osteoarthritis (OA) is the most common joint disorder worldwide
and one of the leading causes of disability in the elderly. We have investigated
the novel sodium hyaluronate derivative chemically linked with diclofenac (DF),
diclofenac etalhyaluronate (SI-613), which is a potentially safer and more
effective treatment for OA knee pain. In this study, we evaluated the
pharmacological effects of SI-613 in experimental arthritis models. METHODS: We
compared the analgesic and anti-inflammatory effects of intra-articularly
administered SI-613, hyaluronic acid (HA), and of orally administered diclofenac
sodium (DF-Na) in rat silver nitrate-induced arthritis model and rabbit antigen-
induced arthritis model. RESULTS: A single intra-articular (IA) administration of
SI-613 significantly suppressed pain responses in rats in a dose-dependent manner.
The analgesic effects were greater than those of HA, a mixture of DF-Na and HA, or
an oral once-daily administration of DF-Na. In the rabbit arthritis model, SI-613
significantly reduced knee joint swelling compared with that in the control group
on day 1 after a single IA injection. This significant anti-inflammatory effect was
observed until day 28. In the pharmacokinetic study, the DF concentration in the
synovium after SI-613 administration reached its maximum concentration of
311.6 ng/g on day 1, and gradually declined to 10 ng/g by day 28. It fell below the
lower limit of quantification on day 35. Thus, a clear correlation was found
between pharmacokinetics and pharmacodynamics. These results demonstrate that SI-
613 exerts its long-lasting and potent anti-inflammatory effect by sustainable
release of DF in the knee joint tissues. CONCLUSION: A single IA injection of SI-
613 was shown to exert analgesic and anti-inflammatory effects for 28 days in non-
clinical pharmacological studies, suggesting that SI-613 will be a promising
candidate in the treatment of osteoarthritis pain.
AN - rayyan-553782273
AU - Yoshioka, K.
AU - Kisukeda, T.
AU - Zuinen, R.
AU - Yasuda, Y.
AU - Miyamoto, K.
DO - 10.1186/s12891-018-2077-8
IS - 1
J2 - BMC Musculoskelet Disord
KW - Animals
Anti-Inflammatory Agents, Non-Steroidal/*administration & dosage/chemistry
Arthritis, Experimental/chemically induced/*drug therapy/pathology
Diclofenac/*administration & dosage/*analogs & derivatives
Hyaluronic Acid/*administration & dosage/*analogs & derivatives
Injections, Intra-Articular
Male
Rabbits
Rats
Treatment Outcome
Diclofenac
LA - eng
N1 - Central Research Lab., Research & Development Div., Seikagaku Corporation,
1253, Tateno 3-chome, Higashiyamato-shi, Tokyo, 207-0021, Japan.
keiji.yoshioka@seikagaku.co.jp.; Central Research Lab., Research & Development
Div., Seikagaku Corporation, 1253, Tateno 3-chome, Higashiyamato-shi, Tokyo, 207-
0021, Japan.; Central Research Lab., Research & Development Div., Seikagaku
Corporation, 1253, Tateno 3-chome, Higashiyamato-shi, Tokyo, 207-0021, Japan.;
Central Research Lab., Research & Development Div., Seikagaku Corporation, 1253,
Tateno 3-chome, Higashiyamato-shi, Tokyo, 207-0021, Japan.; Central Research Lab.,
Research & Development Div., Seikagaku Corporation, 1253, Tateno 3-chome,
Higashiyamato-shi, Tokyo, 207-0021, Japan.
PY - 2018
SP - 157
ST - Pharmacological effects of N-[2-[[2-[2-[(2,6-
dichlorophenyl)amino]phenyl]acetyl]oxy]ethyl]hyaluronamide (diclofenac
Etalhyaluronate, SI-613), a novel sodium hyaluronate derivative chemically linked
with diclofenac
T2 - BMC musculoskeletal disorders
TI - Pharmacological effects of N-[2-[[2-[2-[(2,6-
dichlorophenyl)amino]phenyl]acetyl]oxy]ethyl]hyaluronamide (diclofenac
Etalhyaluronate, SI-613), a novel sodium hyaluronate derivative chemically linked
with diclofenac
VL - 19
Y2 - 5 y3 - 22
ID - 10183
ER -
TY - JOUR
AB - Cardiovascular disease (CVD) is the leading cause of death worldwide. A
search for more effective treatments of CVD is increasingly needed. Major advances
in nanotechnology opened new avenues in CVD therapeutics. Owing to their special
properties, iron oxide, gold and silver nanoparticles (NPs) could exert various
effects in the management and treatment of CVD. The role of iron oxide NPs in the
detection and identification of atherosclerotic plaques is receiving increased
attention. Moreover, these NPs enhance targeted stem cell delivery, thereby
potentiating the regenerative capacity at the injured sites. In addition to their
antioxidative and antihypertrophic capacities, gold NPs have also been shown to be
useful in the identification of plaques and recognition of inflammatory markers.
Contrary to first reports suggestive of their cardio-vasculoprotective role, silver
NPs now appear to exert negative effects on the cardiovascular system. Indeed,
these NPs appear to negatively modulate inflammation and cholesterol uptake, both
of which exacerbate atherosclerosis. Moreover, silver NPs may precipitate
bradycardia, conduction block and sudden cardiac death. In this review, we dissect
the cellular responses and toxicity profiles of these NPs from various perspectives
including cellular and molecular ones. © 2021 The Authors
AN - rayyan-553781676
AU - Younis, N. K.
AU - Ghoubaira, J. A.
AU - Bassil, E. P.
AU - Tantawi, H. N.
AU - Eid, A. H.
DO - 10.1016/j.nano.2021.102433
KW - Atherosclerosis
Cardiovascular disease
Gold nanoparticles
Iron oxide nanoparticles
Nanomedicine
Biomarkers
Cardiovascular Diseases
Ferric Compounds
Gold
Humans
Metal Nanoparticles
Silver
Cardiology
Cardiovascular system
Iron oxides
Metal nanoparticles
Pathology
Stem cells
beta adrenergic receptor blocking agent
biological marker
chitosan derivative
contrast medium
dextran derivative
dipeptidyl carboxypeptidase inhibitor
ferumoxytol
gadolinium
gold nanoparticle
hydroxymethylglutaryl coenzyme A reductase inhibitor
macrogol
metal nanoparticle
silver nanoparticle
superparamagnetic iron oxide
ferric ion
ferric oxide
gold
silver
Atherosclerotic plaque
Cholesterol uptakes
Detection and identifications
Regenerative capacity
Special properties
Sudden cardiac deaths
atherosclerosis
bradycardia
cardiotoxicity
cholesterol transport
drug coating
drug cytotoxicity
heart block
heart failure
heart protection
heart ventricle remodeling
human
iron deficiency anemia
liver toxicity
mesenchymal stem cell transplantation
nanomedicine
nanotechnology
nonhuman
regenerative ability
Review
risk factor
sudden cardiac death
blood
Diseases
PY - 2021
ST - Metal-based nanoparticles: Promising tools for the management of
cardiovascular diseases
TI - Metal-based nanoparticles: Promising tools for the management of
cardiovascular diseases
85110052474&doi=10.1016%2fj.nano.2021.102433&partnerID=40&md5=9ae0ce6f54f2388f68de4
72383a0a627
VL - 36
ID - 9612
ER -
TY - JOUR
AB - Purpose: Mesoporous silica (MSNs) have attracted considerable attention for
its application in the field of drug delivery and biomedicine due to its high
surface area, large pore volume, and low toxicity. Recently, numerous studies
revealed that gut microbiota is of critical relevance to host health. However, the
toxicological studies of MSNs were mainly based on the degradation,
biodistribution, and excretion in mammalian after oral administration for now. Here
in this study, we explored the impacts of oral administration of three kinds of
MSNs on gut microbiota in rats to assess its potential toxicity. Methods: Forty
rats were divided into four groups: control group; Mobil Composition of Matter No.
41 type mesoporous silica (MCM-41) group; Santa Barbara Amorphous-15 type
mesoporous silica (SBA-15) group, and biodegradable dendritic center-radial
mesoporous silica nanoparticle (DMSN) group. Fecal samples were collected 3 days
and 7 days after the intake of MSNs and analyzed with high throughput sequencing.
Gastric tissues in rats were obtained after dissection for the histological study.
Results: Three different MSNs (MCM-41, SBA-15, and DMSN) were successfully prepared
in this study. The pore size of three MSNs was calculated similarly as (3.54 +/-
0.15) nm, (3.48 +/- 0.21) nm, and (3.45 +/- 0.17) nm according to the BET & BJH
model, respectively, while the particle size of MCM-41, SBA-15 and DMSN was around
209.2 nm, 1349.56 nm, and 244.4 nm, respectively. In the gene analysis of 16S rRNA,
no significant changes in the diversity and richness were found between groups,
while Verrucomicrobia decreased and Candidatus Saccharibacteria increased in MCM-41
treated groups. Meanwhile, no inflammatory and erosion symptoms were observed in
the morphological analysis of the colons, except the MCM-41 treated group.
Conclusion: Three different MSNs, MCM-41, SBA-15, and DMSN were successfully
prepared, and this study firstly suggested the impact of MSNs on the gut
microbiota, and further revealing the potential pro-inflammatory effects of oral
administration of MCM-41 was possibly through the changing of gut microbiota.
AN - rayyan-553781678
AU - Yu, Y.
AU - Wang, Z.
AU - Wang, R.
AU - Jin, J.
AU - Zhu, Y. Z.
DO - 10.2147/IJN.S295575
Rats
Inflammation
PY - 2021
SN - 1178-2013
SP - 881-893
ST - Short-Term Oral Administration of Mesoporous Silica Nanoparticles Potentially
Induced Colon Inflammation in Rats Through Alteration of Gut Microbiota
TI - Short-Term Oral Administration of Mesoporous Silica Nanoparticles Potentially
Induced Colon Inflammation in Rats Through Alteration of Gut Microbiota
VL - 16
ID - 9614
ER -
TY - JOUR
AB - A histologie study was made of tho response of the leptomeninges and
underlying cerebral cortex of the cat to subdural implantation of 3 insulating
materials (HR605-P, Parylene* * Trade name of Union Carbide Corp.-C and PI-2555)
and a polymeric electrode component (MMA/MAPTAC) for periods of 8 and 16 wk. The
tissue reactions were compared with those elicited by the arrays of Dacron† †
Dacron® is a registered trade name of Dupont Chemical Corp. mesh matrices, pure
platinum controls and by positive controls (Ag-AgCI) known to cause reactions in
the brain. Sites beneath the Dacron mesh matrix, pure platinum control implants and
beneath all insulating materials implanted for 8 and 16 wk appeared
indistinguishable, exhibiting little tissue reaction. All neurons appeared normal.
The leptomeninges and cortex beneath the Ag-AgCI implants showed a chronic
inflammatory reaction after 8 and 16 wk. Despite varying amounts of oedema, gliosis
and ingrowth of connective tissue in the molecular layer, virtually all underlying
neurons appeared normal. © 1987.
AN - rayyan-553781679
AU - Yuen, T. G. H.
AU - Agnew, W. F.
AU - Bullara, L. A.
DO - 10.1016/0142-9612(87)90103-7
IS - 2
Implants
neuroprosthetic implants
nonporous polymers
silver chloride
subdural space
Animal
Brain
Cats
Cerebral Cortex
Female
Foreign-Body Reaction
Male
Meninges
Polymers
Subdural Space
Support, U.S. Gov't, P.H.S.
celluloid
dacron
hr 605p
methacrylic acid methyl ester
parylene c
platinum
unclassified drug
animal experiment
brain cortex
cat
drug tolerance
electrode
histology
leptomeninx
nervous system
nonhuman
PY - 1987
SP - 138-141
ST - Tissue response to potential neuroprosthetic materials implanted subdurally
T2 - Biomaterials
TI - Tissue response to potential neuroprosthetic materials implanted subdurally
0023141992&doi=10.1016%2f0142-9612%2887%2990103-
7&partnerID=40&md5=0b32564d4f49cc3cca939cd8b3e52f0b
VL - 8
ID - 9615
ER -
TY - JOUR
AB - Introduction: CD4+CD25+ Foxp3+ T regulatory cell (Tregs) represents
approximately 8-10% of the total CD4+ T cell population and are important for
immune homeostasis and preventing autoimmune development. Thus, harnessing their
functions as immune modulator may be coupled with the rapid advancement of
nanotechnology development. Plant-mediated biosynthesis of silver nanoparticle
(AgNP) is noteworthy due to simplicity, rapid rate and potentially render more
biocompatibility with biomolecules. This study identified the effect of
biosynthesized-AgNPs from Garcinia atroviridis (GA) in modulating inflammatory
properties of Treg cells in Non-Obese Resistant (NOR). GA extract was used to
biosynthesized AgNPs and was tested on the effect of inducing inflammatory
properties in CD4+IL17Rhigh cells following 72hr in vitro treatment. Methods:
Conventional CD4+CD25-Foxp3- cells from female NOR mice were sorted using magnetic
separation and cultured in RPMI in the presence of anti-CD3/CD28 antibodies, TGF-β
and IL-2 cytokines. Cells were then treated with or without GA-AgNPs for 48hr of
iTreg cell induction and then re-cultured with new media treated with respective
treatments received. After 72hr in vitro culture, cells were stained with
fluorochrome-conjugated antibodies for flow cytometry. Results: Current result
showed that AgNPs suppress CD4 expression in CD4+IL17Rhigh population. MAPK pathway
proteins remain unchanged in both control and AgNP-treated groups. Conclusions: The
preliminary findings may suggest the properties of GA-AgNPs in modulating CD4+ T
cell population in normal condition. Further studies are necessary to elucidate the
molecular mechanisms involve in such interaction. Current findings serve as basis
in further identifying the immunomodulatory profile of nanoparticle for potential
therapeutic use. © 2018 UPM Press. All rights reserved.
AN - rayyan-553781680
AU - Yusof, N. A. M.
AU - Lee, P. C.
AU - Kamal, N. N. S. N. M.
AU - Zulkifli, N. I.
AU - Ahmad, N. H.
AU - Omar, W. A. W.
AU - Mohamed, R.
AU - Effa, S. Z. N.
KW - Gardinia atriviridis
Immunomodulator
Nanoparticle
Pro-inflammatory
T-regulatory cells
Obesity
PY - 2018
SP - 88-94
ST - Antagonistic effect of biosynthesized AgNPs from garcinia
atroviridisextractonanti-inflammatorypropertiesoFCD4+Ilrhigh cells from non obese
resistance (NOR) mouse model
T2 - Malaysian Journal of Medicine and Health Sciences
TI - Antagonistic effect of biosynthesized AgNPs from garcinia
atroviridisextractonanti-inflammatorypropertiesoFCD4+Ilrhigh cells from non obese
resistance (NOR) mouse model
85068605717&partnerID=40&md5=095452659d6f7bf2b5fcc8b99c38063d
VL - 14
ID - 9616
ER -
TY - JOUR
AB - Dentists used silver-containing solutions for deep cavity disinfection before
restoration. This review aims to identify the silver-containing solutions reported
in the literature for deep cavity disinfection and summarize their effects on
dental pulp. An extensive search was performed using the search words "(silver) AND
(dental pulp OR pulp)" in ProQuest, PubMed, SCOPUS, and Web of Science to identify
English publications on silver-containing solutions for cavity conditioning. The
pulpal response to the included silver-containing solutions was summarized. The
initial search identified 4112 publications and 14 publications met the inclusion
criteria. Silver fluoride, silver nitrate, silver diamine nitrate, silver diamine
fluoride, and nano-silver fluoride were used in deep cavities for antimicrobial
purposes. Indirect silver fluoride application induced pulp inflammation and
reparative dentine in most cases, and pulp necrosis in some cases. Direct silver
nitrate application caused blood clots and a wide inflammatory band in the pulp,
whilst indirect silver nitrate application caused hypoplasia in shallow cavities
and partial pulp necrosis in deep cavities. Direct silver diamine fluoride
application induced pulp necrosis, while indirect silver diamine fluoride
application induced a mild inflammatory response and reparative dentine formation.
No evidence of the dental pulpal response to silver diamine nitrate or nano-silver
fluoride was available in the literature.
AN - rayyan-553781682
AU - Zaeneldin, A.
AU - Chu, C. H.
AU - Yu, O. Y.
DO - 10.3390/dj11050114
IS - 5
KW - Dental Pulp
PY - 2023
SN - 2304-6767
ST - Dental Pulp Response to Silver-Containing Solutions: A Scoping Review
T2 - DENTISTRY JOURNAL
TI - Dental Pulp Response to Silver-Containing Solutions: A Scoping Review
VL - 11
Y2 - 4 y3 - 26
ID - 9618
ER -
TY - JOUR
AB - OBJECTIVE: This study aims to review systematically the dental pulp response
to silver diamine fluoride (SDF) treatment, including the inflammatory response,
pulp cells activity, dentinogenesis, silver penetration, and the presence of the
bacteria in the dental pulp. DATA: In vitro studies, animal studies, clinical
studies, and case reports on the use of SDF on vital dental pulp were included.
Quality assessment of the included studies was conducted. A narrative synthesis of
the collected data was performed. SOURCES: A systematic search was performed in
ProQuest, PubMed, SCOPUS, and Web of Science databases for articles published from
inception to Nov 1, 2021. STUDY SELECTION: The initial search identified 1,433
publications, of which five publications met the inclusion criteria. These five
publications reported the effect of direct/ indirect SDF application on the vital
pulp of a total of 30 teeth. Direct SDF application on vital pulp caused pulp
necrosis. Indirect SDF application caused none or mild inflammatory response of
dental pulp. The odontoblasts in the dental pulp showed increased cellular
activity. Tertiary dentine was formed in the pulpal side of the cavity with
indirect SDF application. Accentuated incremental lines of tertiary dentine
reflected disturbances in mineralisation. Silver ions were found to penetrate along
the dentinal tubules but were not detected inside the pulp. CONCLUSION: According
to the limited available literature, direct SDF application causes pulp necrosis.
Indirect SDF application is generally biocompatible to dental pulp tissue with a
mild inflammatory response, increased odontoblastic activity, and increased
tertiary dentine formation. Future studies with precise quantitative and
qualitative tests, larger sample size and longer follow-up time are imperative to
understand the biological activity of dental pulp to SDF treatment.
AN - rayyan-553781760
AU - Zaeneldin, A.
AU - Yu, O. Y.
AU - Chu, C. H.
DO - 10.1016/j.jdent.2022.104066
J2 - J Dent
KW - Animals
*Dental Caries/drug therapy
Dental Pulp
Dental Pulp Necrosis
*Dentin, Secondary
Fluorides, Topical/pharmacology/therapeutic use
Quaternary Ammonium Compounds/pharmacology/therapeutic use
Silver Compounds/pharmacology/therapeutic use
Fluorides
LA - eng
N1 - Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong
Kong, S.A.R., China.; Faculty of Dentistry, The University of Hong Kong, 34
Hospital Road, Hong Kong, S.A.R., China. Electronic address: ollieyu@hku.hk.;
Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong,
S.A.R., China.
PY - 2022
SP - 104066
ST - Effect of silver diamine fluoride on vital dental pulp: A systematic review
T2 - Journal of dentistry
TI - Effect of silver diamine fluoride on vital dental pulp: A systematic review
VL - 119
Y2 - 4
ID - 9693
ER -
TY - JOUR
AB - Insertions of orthopedic implants are traumatic procedures that trigger an
inflammatory response. Macrophages have been shown to liberate gold ions from
metallic gold. Gold ions are known to act in an antiinflammatory manner by
inhibiting cellular NF-kappaB-DNA binding and suppressing I-kappa B-kinase
activation. The present study investigated whether gilding implant surfaces
augmented early implant osseointegration and implant fixation by its modulatory
effect on the local inflammatory response. Ion release was traced by
autometallographic silver enhancement. Gold-coated cylindrical porous coated
Ti6Al4V implants were inserted press-fit in the proximal part of tibiae in nine
canines and control implants without gold inserted contralateral. Observation time
was 4 weeks. Biomechanical push-out tests showed that implants with gold coating
had approximately 50% decrease in mechanical strength and stiffness.
Histomorphometrical analyses showed gold-coated implants had a decrease in overall
total bone-to-implant contact of 35%. Autometallographic analysis revealed few
cells loaded with gold close to the gilded implant surface. The findings
demonstrate that gilding of implants negatively affects mechanical strength and
osseointegration because of a significant effect of the released gold ions on the
local inflammatory process around the implant. The possibility that a partial
metallic gold coating could prolong the period of satisfactory mechanical strength,
however, cannot be excluded.
AN - rayyan-553782218
AU - Zainali, K.
AU - Danscher, G.
AU - Jakobsen, T.
AU - Jakobsen, S. S.
AU - Baas, J.
AU - Møller, P.
AU - Bechtold, J. E.
AU - Soballe, K.
DO - 10.1002/jbm.a.31924
IS - 1
KW - Alloys
Animals
Cells/drug effects
*Coated Materials, Biocompatible/adverse effects/therapeutic use
Dogs
Gold/*pharmacology/therapeutic use
*Implants, Experimental
Inflammation/drug therapy
Mechanical Phenomena
Osseointegration/drug effects
Tibia
*Titanium/therapeutic use
LA - eng
N1 - Orthopaedic Research Center, Department of Orthopaedics, Aarhus University
Hospital, Noerrebrogade 44, Building 1A, DK-8000 Aarhus C, Denmark.
kza@studmed.au.dk
PY - 2009
SP - 274-80
ST - Effects of gold coating on experimental implant fixation
TI - Effects of gold coating on experimental implant fixation
VL - 88
Y2 - 1
ID - 10128
ER -
TY - JOUR
AB - Due to their unique physicochemical properties, engineered nanoparticles
(NPs) are used in numerous skin products as sunscreen, texture agents, colorant,
and drug delivery systems. While the skin is considered the first line of defense
against xenobiotic entrance, the small size of NPs could promote the interaction
with cutaneous cells. This review investigates the fate and the toxicological
effects of organic and inorganic NPs used in cosmetic and dermatology. After direct
exposure to skin cells, cytotoxicity, oxidative stress, inflammatory response, and
genotoxicity were reported in a dose and time-dependent manner, especially for
inorganic NPs. Despite these findings, the toxicity of nanoparticles applied to a
healthy skin could be questioned due to their inability, for most of them, to reach
the viable epidermis. Advanced skin models and toxicity tests validated for
nanomaterials should be required for a better prediction of the dermal
nanotoxicity. © 2022, The Author(s), under exclusive licence to Springer Nature
B.V.
AN - rayyan-553781685
AU - Zaiter, T.
AU - Cornu, R.
AU - El Basset, W.
AU - Martin, H.
AU - Diab, M.
AU - Béduneau, A.
DO - 10.1007/s11051-022-05523-2
IS - 7
KW - 3D skin models
Cytotoxicity
Genotoxicity
Inflammation
Nanoparticles
Skin barrier
Drug delivery
Textures
antiinfective agent
cytokine
gold nanoparticle
nanoparticle
nickel nanoparticle
silica nanoparticle
silver nanoparticle
3d skin model
Drug-delivery systems
Genotoxicities
Skin model
Toxicity assessment
cytotoxicity
dermatitis
ex vivo study
genotoxicity
human
in vitro study
inflammation
keratinocyte
nonhuman
oxidative stress
Review
skin cell
skin penetration
skin toxicity
time factor
toxicity testing
Skin
PY - 2022
ST - Toxicity assessment of nanoparticles in contact with the skin
TI - Toxicity assessment of nanoparticles in contact with the skin
85133921597&doi=10.1007%2fs11051-022-05523-
2&partnerID=40&md5=e1c70e5f232ac383591bfa8a749d155e
VL - 24
ID - 9621
ER -
TY - JOUR
AB - Due to their unique physicochemical properties, engineered nanoparticles
(NPs) are used in numerous skin products as sunscreen, texture agents, colorant,
and drug delivery systems. While the skin is considered the first line of defense
against xenobiotic entrance, the small size of NPs could promote the interaction
with cutaneous cells. This review investigates the fate and the toxicological
effects of organic and inorganic NPs used in cosmetic and dermatology. After direct
exposure to skin cells, cytotoxicity, oxidative stress, inflammatory response, and
genotoxicity were reported in a dose and time-dependent manner, especially for
inorganic NPs. Despite these findings, the toxicity of nanoparticles applied to a
healthy skin could be questioned due to their inability, for most of them, to reach
the viable epidermis. Advanced skin models and toxicity tests validated for
nanomaterials should be required for a better prediction of the dermal
nanotoxicity.
AN - rayyan-553781686
AU - Zaiter, T.
AU - Cornu, R.
AU - El Basset, W.
AU - Martin, H.
AU - Diab, M.
AU - Beduneau, A.
DO - 10.1007/s11051-022-05523-2
IS - 7
KW - Skin
PY - 2022
SN - 1388-0764 1572-896X
ST - Toxicity assessment of nanoparticles in contact with the skin
TI - Toxicity assessment of nanoparticles in contact with the skin
VL - 24
Y2 - 7
ID - 9622
ER -
TY - JOUR
AB - Driven by the need to biosynthesize alternate biomedical agents to prevent
and treat infection, silver nanoparticles have surfaced as a promising avenue.
Cyanobacteria-derived nanomaterial synthesis is of substantive interest as it
offers an eco-friendly, cost-effective, sustainable, and biocompatible route for
further development. In the present study optimal conditions for synthesis of
silver nanoparticles (AgNPs) were 1 : 9 v/v [cell extract: AgNO3 (1 mM)], pH 7.4,
and 30 °C reaction temperatures. Synthesis of nanoparticles was monitored by UV-vis
spectrophotometry and the maximum absorbance was observed at a wavelength of 420
nm. SEM with EDX analysis confirmed 96.85% silver by weight which revealed the
purity of AgNPs. TEM & XRD analysis exhibited a particle size of ∼12 nm with
crystalline nature. FTIR analysis confirmed the presence of possible biomolecules
involved in the synthesis and stabilization of AgNPs. Decapping of AgNPs followed
by SDS-PAGE, LCMS and MALDI TOF analysis elucidates the proteinaceous nature of the
capping and stabilizing agent. Cyanobacterial-derived capped AgNPs showed more
cytotoxicicity towards a non-small cell lung cancer (A549) cell line, free radical
scavenger and an antimicrobial than de-capped AgNPs. In addition they showed
significant synergistic characteristics with antibiotics and fungicides. The test
revealed that the capped AgNPs were biocompatible with good anti-inflammatory
properties. The blend of antimicrobial and biocompatible properties, coupled with
their intrinsic “green” and facile synthesis, made these biogenic nanoparticles
particularly attractive for future applications in nanomedicine. © The Royal
Society of Chemistry
AN - rayyan-553781687
AU - Zaki, A.
AU - Aziz, M. N.
AU - Ahmad, R.
AU - Ahamad, I.
AU - Ali, M. S.
AU - Yasin, D.
AU - Afzal, B.
AU - Ali, S. M.
AU - Chopra, A.
AU - Hadda, V.
AU - Srivastava, P.
AU - Kumar, R.
AU - Fatma, T.
DO - 10.1039/d1ra08396a
IS - 4
KW - Cell culture
Cost effectiveness
Free radicals
Metal nanoparticles
Microorganisms
Particle size
Silver compounds
Cell extracts
Cost effective
Cyanobacterium
Eco-friendly
EDX analysis
Further development
Maximum absorbance
Optimal conditions
Reaction temperature
UV-vis spectrophotometry
Biocompatibility
PY - 2022
SP - 2497-2510
ST - Synthesis, purification and characterization of
T2 - RSC Advances
TI - Synthesis, purification and characterization of
85123920658&doi=10.1039%2fd1ra08396a&partnerID=40&md5=5ac27257cd919026c3ed931356ede
066
VL - 12
ID - 9623
ER -
TY - JOUR
AB - Driven by the need to biosynthesize alternate biomedical agents to prevent
and treat infection, silver nanoparticles have surfaced as a promising avenue.
Cyanobacteria-derived nanomaterial synthesis is of substantive interest as it
offers an eco-friendly, cost-effective, sustainable, and biocompatible route for
further development. In the present study optimal conditions for synthesis of
silver nanoparticles (AgNPs) were 1 : 9 v/v [cell extract: AgNO3 (1 mM)], pH 7.4,
and 30 degrees C reaction temperatures. Synthesis of nanoparticles was monitored by
UV-vis spectrophotometry and the maximum absorbance was observed at a wavelength of
420 nm. SEM with EDX analysis confirmed 96.85% silver by weight which revealed the
purity of AgNPs. TEM & XRD analysis exhibited a particle size of similar to 12 nm
with crystalline nature. FTIR analysis confirmed the presence of possible
biomolecules involved in the synthesis and stabilization of AgNPs. Decapping of
AgNPs followed by SDS-PAGE, LCMS and MALDI TOF analysis elucidates the
proteinaceous nature of the capping and stabilizing agent. Cyanobacterial-derived
capped AgNPs showed more cytotoxicicity towards a non-small cell lung cancer (A549)
cell line, free radical scavenger and an antimicrobial than de-capped AgNPs. In
addition they showed significant synergistic characteristics with antibiotics and
fungicides. The test revealed that the capped AgNPs were biocompatible with good
anti-inflammatory properties. The blend of antimicrobial and biocompatible
properties, coupled with their intrinsic "green" and facile synthesis, made these
biogenic nanoparticles particularly attractive for future applications in
nanomedicine.
AN - rayyan-553781688
AU - Zaki, A.
AU - Aziz, M. N.
AU - Ahmad, R.
AU - Ahamad, I.
AU - Ali, M. S.
AU - Yasin, D.
AU - Afzal, B.
AU - Ali, S. M.
AU - Chopra, A.
AU - Hadda, V.
AU - Srivastava, P.
AU - Kumar, R.
AU - Fatma, T.
DO - 10.1039/d1ra08396a
IS - 4
PY - 2022
SN - 2046-2069
SP - 2497-2510
ST - Synthesis, purification and characterization of Plectonema derived AgNPs with
elucidation of the role of protein in nanoparticle stabilization
T2 - RSC ADVANCES
TI - Synthesis, purification and characterization of Plectonema derived AgNPs with
elucidation of the role of protein in nanoparticle stabilization
VL - 12
Y2 - 1 y3 - 12
ID - 9624
ER -
TY - JOUR
AB - Nanosized particles including nanovaccines are a novel approach to the
development of vaccines to combat diseases. Nanovaccines have the promise to
utilize the immune system to cure infections and to prevent infections and diseases
from spreading. Rational vaccine development requires an understanding of vaccine
mediated stimulation of the immune system. We review here immunostimulatory
properties of nanovaccines including their immunogenicity, adjuvant properties,
inflammatory responses and the mechanisms of uptake and stimulation of immune
cells. Examples of various nanoparticles currently being developed as vaccines are
also provided. © 2013 Elsevier Inc.
AN - rayyan-553781689
AU - Zaman, M.
AU - Good, M. F.
AU - Toth, I.
DO - 10.1016/j.ymeth.2013.04.014
IS - 3
KW - Adjuvant
Antigen uptake
Delivery system
Immune system
Nanoparticles
Vaccines
Adjuvants, Immunologic
Antigens
Bacterial Vaccines
Dendrimers
Fullerenes
Humans
Immune System
Liposomes
Metal Nanoparticles
Nanotubes
Viral Vaccines
chitosan
gold nanoparticle
macrogol
nanomaterial
nanovaccine
quantum dot
silver nanoparticle
unclassified drug
vaccine
antibody titer
antigen recognition
antigenicity
article
biocompatibility
cell stimulation
cell structure
cellular immunity
deacetylation
dendritic cell
endocytosis
gene delivery system
human
humoral immunity
hydrophobicity
immunization
immunogenicity
immunostimulation
innate immunity
internalization
nanotechnology
nonhuman
oxidative stress
particle size
physical chemistry
priority journal
surface charge
PY - 2013
SP - 226-231
ST - Nanovaccines and their mode of action
T2 - Methods
TI - Nanovaccines and their mode of action
84878821247&doi=10.1016%2fj.ymeth.2013.04.014&partnerID=40&md5=9e0833f78e622fb02eb5
e0f7b26825d5
VL - 60
ID - 9625
ER -
TY - JOUR
AB - There is an increasing commercial demand for nanoparticles due to their wide
applicability in various areas such as electronics, catalysis, chemistry, energy,
and medicine. Recently, researchers have tried to synthesize the chemotherapeutic
drugs from metallic nanoparticles especially gold and silver nanoparticles. In the
current study, silver nanoparticles using Spinacia oleracea L. leaf aqueous extract
(AgNPs) are reported for the first time to exert a dietary remedial property
compared to doxorubicin in an animal model of acute myeloid leukemia. The
synthesized AgNPs were characterized using different techniques including UV-Vis.,
EDS, TEM, FT-IR, and FE-SEM. UV-Vis. indicates an absorption band at 462 nm that is
related to the surface plasmon resonance of AgNPs. In EDS, metallic silver
nanocrystals indicated an optical absorption peak at roughly 4keV. TEM and FE-SEM
images exhibited a uniform spherical morphology and diameters of 20–40 nm for the
nanoparticles. FT-IR findings suggested antioxidant compounds in the nanoparticles
were the sources of reducing power, reducing silver ions to AgNPs. In vivo design,
induction of acute myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene in
75 mice. Then, the animals were randomly divided into six subgroups, including
control, untreated, AgNO3, S. oleracea, AgNPs, and doxorubicin. Similar to
doxorubicin, AgNPs significantly (p ≤ 0.01) reduced the pro-inflammatory cytokines,
and the total WBC, blast, neutrophil, monocyte, eosinophil, and basophil counts and
increased the weight of the body, the anti-inflammatory cytokines and the
lymphocyte, platelet, and RBC parameters as compared to the untreated mice. DPPH
free radical scavenging test was done to evaluate the antioxidant potentials of
AgNO3, S. oleracea, AgNPs, and doxorubicin. DPPH test revealed similar antioxidant
potentials for doxorubicin and AgNPs. For the analyzing of cytotoxicity effects of
AgNO3, S. oleracea, AgNPs, and doxorubicin, MTT assay was used on HUVEC, Human HL-
60/vcr, 32D-FLT3-ITD, and Murine C1498 cell lines. AgNPs similar to doxorubicin had
low cell viability dose-dependently against Human HL-60/vcr, 32D-FLT3-ITD, and
Murine C1498 cell lines without any cytotoxicity on HUVEC cell line. These results
reveal that the inclusion of S. oleracea leaf aqueous extract improves the remedial
effects of AgNPs, which led to a significant enhancement in the antioxidant,
cytotoxicity, and anti-acute myeloid leukemia potentials of the nanoparticles. It
seems that AgNPs can be applied as a chemotherapeutic supplement or drug for the
treatment of acute myeloid leukemia in the clinical trial. © 2019 John Wiley &
Sons, Ltd.
AN - rayyan-553781693
DO - 10.1002/aoc.5295
IS - 1
Doxorubicin
leukemic mouse model
Spinacia oleracea L.
Antioxidants
Cell culture
Cells
Chemical analysis
Cytotoxicity
Diseases
Free radicals
Light absorption
Mammals
Metal ions
Metal nanoparticles
Morphology
Silver compounds
Surface plasmon resonance
Chemical characterization
Chemotherapeutic drugs
Mouse models
Spinacia oleracea
Drug delivery
Leukemia, Myeloid
Mice
PY - 2020
ST - Green synthesis and formulation a modern chemotherapeutic drug of Spinacia
oleracea L. leaf aqueous extract conjugated silver nanoparticles; Chemical
characterization and analysis of their cytotoxicity, antioxidant, and anti-acute
myeloid leukemia properties in comparison to doxorubicin in a leukemic mouse model
TI - Green synthesis and formulation a modern chemotherapeutic drug of Spinacia
oleracea L. leaf aqueous extract conjugated silver nanoparticles; Chemical
characterization and analysis of their cytotoxicity, antioxidant, and anti-acute
myeloid leukemia properties in comparison to doxorubicin in a leukemic mouse model
85074818317&doi=10.1002%2faoc.5295&partnerID=40&md5=e224f33dcdfef3e8067fbfcfa187480
c
VL - 34
ID - 9629
ER -
TY - JOUR
AB - This study aimed to investigate the cytotoxicity and pro-inflammatory
responses induced by tungsten disulphide (WS2) and molybdenum disulphide (MoS2)
nanoparticles (NPs) in human bronchial cells (BEAS-2B). For cytotoxicity
assessment, the cells were exposed to different concentrations (2.5-200 mu g/mL) of
WS2-NPs or MoS2-NPs for 24 and 48 h and then the MTT assay was performed.
Afterwards, long-term toxicity was assessed by the colony forming efficiency assay
(CFEA) during a 10 days' exposure of the cells. For pro-inflammatory responses, the
expression of interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) mRNA was
estimated by the real-time PCR method. Both nanomaterials showed similar cytotoxic
effects on BEAS-2B cells assessed by the MTT assay, i.e. reduction in cell
viability to approx. 60-70% at concentrations of 2.5 and 5 mu g/mL after 24 and 48
h. The percentage viability remained relatively constant at this level across all
concentrations above 5 mu g/mL. In long-term exposure, both nanomaterials inhibited
colony formation in a wide range of concentrations up to 100 mu g/mL. MoS2-NPs were
slightly more cytotoxic than WS2-NPs. Additionally, MoS2-NPs caused an increase in
mRNA levels of cytokines, IL-1 beta, and IL-6 at concentration of 50 mu g/mL, while
WS2-NPs did not cause any changes in the level of mRNA for both cytokines. We also
visualised the changes in the cells as a result of WS2-NPs or MoS2-NPs exposure
(2.5 and 25 mu g/mL) via holotomographic microscopy. This work demonstrates the
hazardous potential of both nanomaterials and indicate that WS2 and MoS2
nanoparticles should be included in the occupational risk assessment.
AN - rayyan-553781694
AU - Zapor, L.
AU - Chojnacka-Puchta, L.
AU - Sawicka, D.
AU - Miranowicz-Dzierzawska, K.
AU - Skowron, J.
DO - 10.1515/ntrev-2022-0073
IS - 1
KW - Humanities
Humanism
Humans
PY - 2022
SN - 2191-9089 2191-9097
SP - 1263-1272
ST - Cytotoxic and pro-inflammatory effects of molybdenum and tungsten disulphide
on human bronchial cells
T2 - NANOTECHNOLOGY REVIEWS
TI - Cytotoxic and pro-inflammatory effects of molybdenum and tungsten disulphide
on human bronchial cells
VL - 11
Y2 - 3 y3 - 15
ID - 9630
ER -
TY - JOUR
AB - Today, wound healing is an important clinical problem that is often affected
by microbial infection and not paying attention to this problem can cause
irreparable damage to people. Biosynthesis of metal nanoparticles (NPs) has created
a huge revolution in the field of nanomedicine due to their non-toxic,
biocompatible, and stable characteristics. For this purpose, in this study,
Petroselinum crispum seed extract (PCS) was applied to preparation of silver
nanoparticles (AgNPs@PCS). The optimization of the silver nanoparticle synthesis
involved adjusting the concentration of the silver salt, as well as the time and
temperature parameters. After identifying the fabricated nanoparticles by FESEM,
XRD, FT-IR, UV–Vis, TEM and EDS, their biological activity (like antibacterial,
antifungal, antioxidant, anticancer, and wound treatment) was determined. The green
synthesis of AgNPs was confirmed by several characteristics, including the surface
plasmon response with a peak around 420 nm, the presence of both regular and
heterogeneous sizes in the sample, and the observed color change from a clear
solution to a brown color. The antimicrobial properties of the biofabricated AgNPs
were investigated against fungal and various bacteria. The anticancer property of
AgNPs@PCS was demonstrated by an IC50 value of 200 µg/ml after a 24-hour exposure
on the MCF-7 cell line. Also, a dose-dependent antioxidant performance of AgNPs was
found against the DPPH free radical. Histopathological evaluations of AgNPs@PCS
ointment illustrated significant decrease in inflammatory cells. The results showed
that vaseline ointment containing AgNPs@PCS prevented inflammation in the wound
area and increased the number of fibroblast cells, which led to accelerated wound
healing. Furthermore, in-vivo investigation showed the higher percentage of wound
closure on days 7 and 14 was than the control group (Vaseline). Interestingly, the
wound was completely closed after 21 days. Thus, we concluded that Petroselinum
crispum-mediated silver nanoparticles show potential biological activity which can
be used as nano-drug in clinical treatment. © 2023 The Author(s)
AN - rayyan-553781695
AU - Zare-Bidaki, M.
AU - Ghasempour, A.
AU - Mohammadparast-Tabas, P.
AU - Ghoreishi, S. M.
AU - Alamzadeh, E.
AU - Javanshir, R.
AU - Le, B. N.
AU - Barakchi, M.
AU - Fattahi, M.
AU - Mortazavi-Derazkola, S.
DO - 10.1016/j.arabjc.2023.105194
IS - 10
KW - Antibacterial
Anticancer
Antioxidant
Petroselinum crispum
Silver nanoparticle
Wound healing
Antioxidants
Bioactivity
Biocompatibility
Cell culture
Free radicals
Fungi
Metal nanoparticles
Surface plasmons
Antibacterials
Antifungal activities
Antioxidant and anticancer activities
Clinical problems
In-vivo
Seeds extracts
Wound Healing
PY - 2023
ST - Enhanced in vivo wound healing efficacy and excellent antibacterial,
antifungal, antioxidant and anticancer activities via AgNPs@PCS
T2 - Arabian Journal of Chemistry
TI - Enhanced in vivo wound healing efficacy and excellent antibacterial,
antifungal, antioxidant and anticancer activities via AgNPs@PCS
85166626527&doi=10.1016%2fj.arabjc.2023.105194&partnerID=40&md5=a32c5251c0bfd8ed73b
844701cf2f0c7
VL - 16
ID - 9631
ER -
TY - JOUR
AB - Multiple sclerosis (MS) is a deleterious autoimmune and demyelinating
disorder of the central nervous system with debilitating sensory and motor
complications. There is still no definite cure for it and the main focus for its
treatment mostly pivots around subsiding its severity and recurrence. Experimental
autoimmune encephalomyelitis (EAE) is an established animal model of MS. S-allyl
cysteine (SAC) is the active and main constituent of aged garlic extract with anti-
inflammatory and neuroprotective property. This study was conducted to evaluate its
possible protective effect in EAE model of MS. SAC was administered p.o. at a dose
of 50 mg/kg/day to female C57BL/6 mice immunized with myelin oligodendrocytic
glycoprotein (MOG(35-55)). Results showed that SAC is capable to alleviate clinical
signs and severity of the disease and improved lumbar spinal cord tissue level of
tumor necrosis factor α (TNFa), interleukin 17 (IL-17), activity-dependent
neuroprotector homeobox (ADNP), microtubule-associated proteins 1A/1 B light chain
3A (MAP1LC3A), and matrix metalloproteinase 9 (MMP-9). In addition, SAC attenuated
inflammatory cell infiltration, axonal demyelination, and axonal loss in lumbar
spinal cord in EAE group, as demonstrated by H & E, Luxol fast blue (LFB), and
Bielschowsky silver staining, respectively. Taken together, SAC could mitigate
severity of MOG(35-55)-induced EAE as a valid model of MS via amelioration of
pathogenic molecular mechanisms responsible for neuroinflammation and axonal
damage.
AN - rayyan-553782327
AU - Zeinali, H.
AU - Baluchnejadmojarad, T.
AU - Fallah, S.
AU - Sedighi, M.
AU - Moradi, N.
AU - Roghani, M.
DO - 10.1016/j.biopha.2017.10.155
J2 - Biomed Pharmacother
KW - Animals
Anti-Inflammatory Agents/pharmacology/*therapeutic use
Cysteine/*analogs & derivatives/pharmacology/therapeutic use
Encephalomyelitis, Autoimmune, Experimental/*drug therapy/metabolism/*pathology
Female
Inflammation Mediators/antagonists & inhibitors
Mice
Mice, Inbred C57BL
Myelin-Oligodendrocyte Glycoprotein/toxicity
Encephalomyelitis, Autoimmune, Experimental
Cysteine
Encephalomyelitis
Autoimmunity
LA - eng
N1 - Department of Physiology, School of Medicine, Iran University of Medical
Sciences, Tehran, Iran.; Department of Physiology, School of Medicine, Iran
University of Medical Sciences, Tehran, Iran. Electronic address:
baluchnejadmojarad.t@iums.ac.ir.; Department of Biochemistry, School of Medicine,
Iran University of Medical Sciences, Tehran, Iran.; Department of Neuroscience,
Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences,
Tehran, Iran.; Department of Biochemistry, School of Medicine, Iran University of
Medical Sciences, Tehran, Iran.; Neurophysiology Research Center, Shahed
University, Tehran, Iran. Electronic address: mroghani@shahed.ac.ir.
PY - 2018
SP - 557-563
ST - S-allyl cysteine improves clinical and neuropathological features of
experimental autoimmune encephalomyelitis in C57BL/6 mice
T2 - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
TI - S-allyl cysteine improves clinical and neuropathological features of
experimental autoimmune encephalomyelitis in C57BL/6 mice
VL - 97
Y2 - 1
ID - 10235
ER -
TY - JOUR
AB - Numerous studies show that nonsteroidal anti-inflammatory drugs (NSAIDs) are
effective in chemoprevention or treatment of cancer. Nevertheless, the mechanisms
underlying these antineoplastic effects remain poorly understood. Here, we report
that induction of the cancer-specific proapoptotic cytokine melanoma
differentiation associated gene-7/interleukin-24 (MDA-7/IL-24) by several NSAIDs is
an essential step for induction of apoptosis and G(2)-M growth arrest in cancer
cells in vitro and inhibition of tumor growth in vivo. We also show that MDA-7/IL-
24-dependent up-regulation of growth arrest and DNA damage inducible 45 alpha
(GADD45alpha) and GADD45gamma gene expression is sufficient for cancer cell
apoptosis via c-Jun NH(2)-terminal kinase (JNK) activation and growth arrest
induction through inhibition of Cdc2-cyclin B checkpoint kinase. Knockdown of
GADD45alpha and GADD45gamma transcription by small interfering RNA abrogates
apoptosis and growth arrest induction by the NSAID treatment, blocks JNK
activation, and restores Cdc2-cyclin B kinase activity. Our results establish MDA-
7/IL-24 and GADD45alpha and GADD45gamma as critical mediators of apoptosis and
growth arrest in response to NSAIDs in cancer cells.
AN - rayyan-553782095
AU - Zerbini, L. F.
AU - Czibere, A.
AU - Wang, Y.
AU - Correa, R. G.
AU - Otu, H.
AU - Joseph, M.
AU - Takayasu, Y.
AU - Silver, M.
AU - Gu, X.
AU - Ruchusatsawat, K.
AU - Li, L.
AU - Sarkar, D.
AU - Zhou, J. R.
AU - Fisher, P. B.
AU - Libermann, T. A.
DO - 10.1158/0008-5472.CAN-06-2068
IS - 24
J2 - Cancer Res
KW - Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use
Apoptosis/*drug effects
Cell Cycle Proteins/genetics
Cell Differentiation/genetics
Cell Division/*drug effects
Cell Line, Tumor
Culture Media
DNA Damage
Humans
Interleukins/*genetics/physiology
Intracellular Signaling Peptides and Proteins/genetics
Male
Nuclear Proteins/genetics
Polymerase Chain Reaction
Prostatic Neoplasms/drug therapy/genetics/pathology
Transcription, Genetic
Melanoma
LA - eng
N1 - BIDMC Genomics Center, Beth Israel Deaconess Medical Center and Harvard
Medical School, Boston, Massachusetts 02115, USA.
PY - 2006
SN - 0008-5472 (Print)
SP - 11922-31
ST - A novel pathway involving melanoma differentiation associated
gene-7/interleukin-24 mediates nonsteroidal anti-inflammatory drug-induced
apoptosis and growth arrest of cancer cells
T2 - Cancer research
TI - A novel pathway involving melanoma differentiation associated
gene-7/interleukin-24 mediates nonsteroidal anti-inflammatory drug-induced
apoptosis and growth arrest of cancer cells
VL - 66
Y2 - 12 y3 - 15
ID - 10006
ER -
TY - JOUR
AB - Nanosilver is one of the most commercialized nanomaterials in the world. Due
to its unique surface plasma resonance performance and excellent antibacterial
activities, nanosilver has been widely used in many fields, such as medical area,
health care, industrial products and our daily supplies. Meanwhile, the increasing
application of nanosilver has drawn more and more attention to its biosafety.
Previous toxicological studies have revealed diverse deleterious effects nanosilver
may cause, wherein, neurotoxicity is highly concerned. This review mainly focuses
on the neurotoxicological effects of nanosilver, and three aspects, including the
bioaccumulation of nanosilver in brain and its penetration routes,
neurotoxicological effects and the underlying molecular mechanisms, and the
influencing factors, are comprehensively discussed. The administration of
nanosilver through diverse ways could cause brain silver accumulation, and its
penetration routes to the brain were mainly involved with the direct nasal
olfactory nerve transfer and the translocation of the blood-brain barrier. The
neurotoxicological effects of nanosilver were evidenced by neurobehavioral changes
in the exposed animals, histopathological alteration in the brain or cellular
morphological changes in neurons and neuroglia cells, and the disturbance in the
neurontransmitter secretion. The underlying mechanisms were related with oxidative
damage and inflammatory responses. The factors, including particle size, surface
coating and silver ion release, would potentially determine nanosilver induced
neurotoxicity. Finally, the existing problems in neurotoxicological studies on
nanosilver are pointed out, and the future perspectives in this area are proposed.
The review would be of great help to risk assessment of the production, application
and disposal of nanosilver.
AN - rayyan-553781697
AU - Zhang, B. J.
AU - Liu, Q. S.
AU - Zhou, Q. F.
AU - Zhang, J. Q.
AU - Jiang, G. B.
DO - 10.7536/PC171228
IS - 9
PY - 2018
SN - 1005-281X
SP - 1392-1402
ST - Neurotoxicological Effects of Nanosilver
T2 - PROGRESS IN CHEMISTRY
TI - Neurotoxicological Effects of Nanosilver
VL - 30
Y2 - 9 y3 - 15
ID - 9632
ER -
TY - JOUR
AB - Drug-induced vascular injury (DIVI) is a nonclinical finding that often
confounds the toxicological evaluation of investigational drugs, but there is an
absence of qualified biomarkers that can be used to detect and monitor its
appearance in animals and patients during drug development and clinical use. It is
well known that endothelial cell (EC) activation plays a key role in the expression
and evolution of DIVI, and the various immunological and inflammatory factors
involved in its expression may serve as potential biomarker candidates. Activated
ECs change their morphology and gene expression, generating endothelial adhesion
molecules, pro-coagulant molecules, cytokines, chemokines, vasodilators, nitric
oxide, and acute-phase reactants. This review provides a brief historical
background of EC activation and the search for biomarkers of early EC activation
for monitoring DIVI. At present, no biomarkers of EC activation have been qualified
to predict DIVI in the nonclinical or clinical context, and a robust pathologic
foundation for their use is still lacking. We propose three categories of EC
activation biomarkers: recommended surrogate markers, potentially useful markers,
and emerging candidate markers. This review alerts pharmaceutical companies,
research institutions, and regulatory agencies to the continuing need for reliable
biomarkers of EC activation in drug development.
AN - rayyan-553781956
AU - Zhang, J.
AU - Defelice, A. F.
AU - Hanig, J. P.
AU - Colatsky, T.
DO - 10.1177/0192623310378866
IS - 6
J2 - Toxicol Pathol
KW - Animals
Drug Evaluation, Preclinical/methods
Endothelium, Vascular/*drug effects/metabolism/pathology
Humans
Vascular Diseases/*chemically induced/metabolism/pathology
Xenobiotics/*toxicity
Biological Markers
LA - eng
N1 - Division of Applied Pharmacology Research, Center for Drug Evaluation and
Research, Food and Drug Administration, Silver Spring, MD 20993, USA.
jun.zhang@fda.hhs.gov
PY - 2010
SP - 856-71
ST - Biomarkers of endothelial cell activation serve as potential surrogate
markers for drug-induced vascular injury
T2 - Toxicologic pathology
TI - Biomarkers of endothelial cell activation serve as potential surrogate
markers for drug-induced vascular injury
VL - 38
Y2 - 10
ID - 9876
ER -
TY - JOUR
AB - The biological properties of the lanthanides, primarily based on their
similarity to calcium, have been the basis for research into potential therapeutic
applications of lanthanides since the early part of the twentieth century. Up to
date, cerium nitrate has been used as a topical cream with silver sulfadiazene for
the treatment of burn wounds. A lanthanide texaphyrin complex (motexafin
gadolinium) has been evaluated through Phase III clinical trials for the treatment
of brain metastases in non-small cell lung cancer. Lanthanum carbonate (Fosrenol)
as a phosphate binder has been approved for the treatment of hyperphosphatemia in
renal dialysis patients in both the USA and Europe. This review will highlight
therapeutic applications of the lanthanides for burn wounds, cancer,
hyperphosphatemia, immune function, magnetic resonance imaging (MRI) contrast
agents and osteoporosis, and discuss their future potential in the medical fields.
© 2011 Bentham Science Publishers Ltd.
AN - rayyan-553781704
AU - Zhang, J.
AU - Li, Y.
AU - Hao, X.
AU - Zhang, Q.
AU - Yang, K.
AU - Li, L.
AU - Ma, L.
AU - Wang, S.
AU - Li, X.
DO - 10.2174/138955711796268804
IS - 8
KW - Burn wounds
Cancer
Hyperphosphatemia
Immune function
Lanthanides
Mri contrast agents
Osteoporosis
Therapeutic application
acetic acid
antibiotic agent
antiinfective agent
cerium
cerium iodide
cerium nitrate
chloride
contrast medium
dermacerium
flammacerium
gadobenate dimeglumine
gadobutrol
gadodiamide
gadofosveset
gadolinium pentetate
gadolinium texaphyrin
gadoterate meglumine
gadoteridol
gadoversetamide
gadoxetic acid
lanthanide
lanthanum carbonate
lanthanum complex
metal complex
metal derivative
neodymium complex
nitrate
stearic acid
sulfadiazine silver
unclassified drug
unindexed drug
warfarin
biocompatibility
brain metastasis
burn
cancer survival
chelation
clinical trial (topic)
complex formation
cytotoxicity
diagnostic imaging
drug efficacy
drug mechanism
drug safety
drug synthesis
drug tolerability
food and drug administration
human
hyperphosphatemia
immune system
immunomodulation
in vitro study
in vivo study
liver protection
lung non small cell cancer
mortality
multiple organ failure
neoplasm
nonhuman
osteoporosis
oxidation reduction state
radioimmunotherapy
review
systemic inflammatory response syndrome
PY - 2011
SP - 678-694
ST - Recent progress in therapeutic and diagnostic applications of lanthanides
T2 - Mini-Reviews in Medicinal Chemistry
TI - Recent progress in therapeutic and diagnostic applications of lanthanides
79960443580&doi=10.2174%2f138955711796268804&partnerID=40&md5=4276033351197697dce3d
883bb77d00b
VL - 11
ID - 9639
ER -
TY - JOUR
AB - Our aims of the research were to study the antimicrobial effect of
dimethylaminododecyl methacrylate (DMADDM) modified denture base resin on multi-
species biofilms and the biocompatibility of this modified dental material. Candida
albicans (C. albicans), Streptococcus mutans (S. mutans), Streptococcus sanguinis
(S. sanguinis), as well as Actinomyces naeslundii (A. naeslundii) were used for
biofilm formation on denture base resin. Colony forming unit (CFU) counts,
microbial viability staining, and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-
tetrazolium-5-carboxanilide (XTT) array were used to evaluate the antimicrobial
effect of DMADDM. C. albicans staining and Real-time PCR were used to analyze the
morphology and expression of virulence genes of C. albicans in biofilm. Lactate
dehydrogenase (LDH) array and Real-time PCR were conducted to examine the results
after biofilm co-cultured with epithelial cell. Hematoxylin and eosin (HE) staining
followed by histological evaluation were used to study the biocompatibility of this
modified material. We found that DMADDM containing groups reduced both biomass and
metabolic activity of the biofilm significantly. DMADDM can also inhibit the
virulence of C. albicans by means of inhibiting the hyphal development and
downregulation of two virulence related genes. DMADDM significantly reduced the
cell damage caused by multi-species biofilm according to the LDH activity and
reduced the expression of IL-18 gene of the cells simultaneously. The in vivo
histological evaluation proved that the addition of DMADDM less than 6.6% in
denture material did not increase the inflammatory response (p > 0.05). Therefore,
we proposed that the novel denture base resin containing DMADDM may be considered
as a new promising therapeutic system against problems caused by microbes on
denture base such as denture stomatitis.
AN - rayyan-553781707
AU - Zhang, K. K.
AU - Ren, B.
AU - Zhou, X. D.
AU - Xu, H. H. K.
AU - Chen, Y.
AU - Han, Q.
AU - Li, B. L.
AU - Weir, M. D.
AU - Li, M. Y.
AU - Feng, M. Y.
AU - Cheng, L.
DO - 10.3390/ijms17071033
IS - 7
PY - 2016
SN - 1422-0067
ST - Effect of Antimicrobial Denture Base Resin on Multi-Species Biofilm Formation
TI - Effect of Antimicrobial Denture Base Resin on Multi-Species Biofilm Formation
VL - 17
Y2 - 7
ID - 9642
ER -
TY - JOUR
AB - In this paper, Ag-Metal-organic framework loaded chitosan nanoparticles
(0.1%Ag@MOF/1.5%CSNPs) and polyvinyl alcohol/sodium alginate/chitosan (PACS) were
used as the upper and lower layers to successfully prepare a bilayer composite
dressing for wound healing. The performance of bilayer dressing was evaluated. The
lower layer (PACS) had uniform pore size distribution, good water retention,
swelling, water vapor permeability, and biocompatibility while PACS had almost no
antibacterial activity. The upper layer (Ag@MOF/CSNPs) possessed excellent
antibacterial activity and poor biocompatibility. As the upper layer, it can avoid
direct contact with the skin and inhibit microbial invasion. In addition, the
bilayer can adhere to a large number of red blood cells and platelets, promoting
blood coagulation and cell proliferation. Ag@MOF, CSNPs, Ag@MOF/CSNPs and bilayer
showed antibacterial activity in ascending order, due to the synergistic
antibacterial action of the upper and lower layer. In vivo evaluation showed that
both bilayer and PACS could significantly accelerate the wound healing, and the
bilayer dressing showed more complete re-epithelialization with less inflammatory
cells. In summary, this new bilayer composite is an ideal dressing for accelerating
wound healing.
AN - rayyan-553782006
AU - Zhang, M.
AU - Wang, G.
AU - Wang, D.
AU - Zheng, Y.
AU - Li, Y.
AU - Meng, W.
AU - Zhang, X.
AU - Du, F.
AU - Lee, S.
DO - 10.1016/j.ijbiomac.2021.02.045
KW - Alginates/chemistry
Animals
Bandages/microbiology
Cell Line
Chitosan/chemistry/*pharmacology
Hydrogels/chemical synthesis/pharmacology
Male
Metal Nanoparticles
Metal-Organic Frameworks/chemistry/pharmacology
Mice
Mice, Inbred BALB C
Polyvinyl Alcohol/chemistry
Skin/drug effects
Wound Healing
Polyvinyls
Alcoholics
Alginates
LA - eng
N1 - College of Environment and Safety Engineering, Qingdao University of Science
and Technology, Qingdao 266042, People's Republic of China; Shandong Engineering
Research Center for Marine Environment Corrosion and Safety Protection, Qingdao
University of Science and Technology, Qingdao 266042, People's Republic of China;
Shandong Engineering Technology Research Center for Advanced Coating, Qingdao
University of Science and Technology, Qingdao 266042, People's Republic of China.;
College of Environment and Safety Engineering, Qingdao University of Science and
Technology, Qingdao 266042, People's Republic of China; Shandong Engineering
University of Science and Technology, Qingdao 266042, People's Republic of China.;
College of Environment and Safety Engineering, Qingdao University of Science and
Technology, Qingdao 266042, People's Republic of China; Shandong Engineering
University of Science and Technology, Qingdao 266042, People's Republic of China.
Electronic address: wd_charrel@163.com.; College of Environment and Safety
Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's
Republic of China; Shandong Engineering Research Center for Marine Environment
Corrosion and Safety Protection, Qingdao University of Science and Technology,
Qingdao 266042, People's Republic of China; Shandong Engineering Technology
Research Center for Advanced Coating, Qingdao University of Science and Technology,
Qingdao 266042, People's Republic of China.; College of Environment and Safety
Corro ion and Safety Protection, Qingdao University of Science and Technology,
Qingdao 266042, People's Republic of China.
PY - 2021
SP - 481-494
ST - Ag@MOF-loaded chitosan nanoparticle and polyvinyl alcohol/sodium
alginate/chitosan bilayer dressing for wound healing applications
TI - Ag@MOF-loaded chitosan nanoparticle and polyvinyl alcohol/sodium
alginate/chitosan bilayer dressing for wound healing applications
VL - 175
Y2 - 4 y3 - 1
ID - 9922
ER -
TY - JOUR
AB - The creation of wound dressings with low drug resistance and broad-spectrum
antibacterial capability is a key topic of scientific interest. To achieve this, a
bactericidal wound dressing with the capacity to autocatalytically produce hydroxyl
radicals ([rad]OH) was developed. The wound dressing was an electrospun
PCL/gelatin/glucose composite fiber mesh (PGD) with functional iron-containing
metal-organic framework (Fe-MOF) nanozymes. These functional nanozymes (G@Fe) were
formed by coupling glucose oxidase (GOx) and Fe-MOF through amide bonds. These
nanozymes enabled the conversion of glucose released from the PGD composite mesh
into hydroxyl radicals via an autocatalytic cascade reaction to destroy bacteria.
The antibacterial efficiency of wound dressings and their stimulation of tissue
regeneration were assessed using a MRSA-infected skin wound infection model on the
back of SD mice. The G@Fe/PGD wound dressing exhibited improved wound healing
capacity and had comparable biosafety to commercial silver-containing dressings,
suggesting a potential replacement in the future. © 2023
AN - rayyan-553781711
AU - Zhang, P.
AU - Xu, X.
AU - He, W.
AU - Li, H.
AU - Huang, Y.
AU - Wu, G.
DO - 10.1016/j.nano.2023.102683
KW - Antibacterial
Autocatalytic
Hydroxyl radical
Metal-organic framework nanozyme
Wound dressing
Animals
Bacteria
Bandages
Glucose
Mice
Wound Healing
Wound Infection
Amides
Glucose oxidase
Glucose sensors
Hydrogen bonds
Iron compounds
Mammals
Mesh generation
Organometallics
Tissue regeneration
collagen
gelatin
glucose
glucose oxidase
hydroxyl radical
iron
nanoparticle
silver
antiinfective agent
Antibacterials
Broad spectrum
Drug-resistance
Hydroxyl radicals
Infected wounds
Wound dressings
Wound healing
animal experiment
animal model
animal tissue
Article
bacterial skin disease
biocompatibility
contact angle
controlled study
electrospinning
Escherichia coli
Escherichia coli infection
fibroblast
hair follicle
heart
high resolution scanning electron microscopy
histopathology
in vitro study
in vivo study
inflammatory cell
kidney
liver
lung
male
mouse
nonhuman
spleen
tissue regeneration
wound healing
wound infection
animal
bacterium
chemistry
microbiology
PY - 2023
ST - Autocatalytically hydroxyl-producing composite wound dressing for bacteria-
infected wound healing
TI - Autocatalytically hydroxyl-producing composite wound dressing for bacteria-
infected wound healing
85156123534&doi=10.1016%2fj.nano.2023.102683&partnerID=40&md5=71acd0ba84dfe622922d9
abfea237421
VL - 51
ID - 9645
ER -
TY - JOUR
AB - It remains challenging to cure chronic diabetic wounds due to its' harsh
microenvironment and poor tissue regeneration ability. At present, bacteria
elimination, inflammatory response suppression and angiogenesis orderly render an
important paradigm for chronic diabetic wound treatment. Herein, smart-responsive
multifunctional hydrogels were developed to improve chronic diabetic wound healing,
which could quickly respond to the acidic environment of the diabetic wound site
and mediate multistage sequential delivery of silver and curcumin-loaded
polydopamine nanoparticles (PDA@Ag&Cur NPs) and vascular endothelial growth factor
(VEGF). PDA@Ag&Cur NPs and VEGF endowed the hydrogels with antibacterial, anti-
inflammatory and angiogenesis performances, respectively. The in vitro and in vivo
experiments confirmed that our multistage drug delivery hydrogels could effectively
eliminate bacteria, relieve inflammatory response, and induce angiogenesis, hence
accelerating the closure of chronic diabetic wounds. In conclusion, we highlighted
the importance of multistage manipulation in wound healing and offered a
combinatorial therapeutic strategy to sequentially deliver drugs exactly aiming at
the dynamic wound healing stages. © 2023
AN - rayyan-553781715
AU - Zhang, W.
AU - Liu, W.
AU - Long, L.
AU - He, S.
AU - Wang, Z.
AU - Liu, Y.
AU - Yang, L.
AU - Chen, N.
AU - Hu, C.
AU - Wang, Y.
DO - 10.1016/j.jconrel.2023.01.049
KW - Combinatorial treatment
Diabetic wound healing
Multistage sequential drug delivery
Smart-responsive hydrogels
Bacteria
Diabetes Mellitus
Humans
Hydrogels
Wound Healing
Tissue regeneration
beta actin
curcumin
hydrogel
interleukin 6
silver nanoparticle
vasculotropin
antiinfective agent
vasculotropin A
Angiogenesis
Diabetic wounds
Multi-stages
Responsive hydrogels
Smart-responsive hydrogel
Wound healing
angiogenesis
animal experiment
animal model
aqueous solution
Article
cell migration
cell proliferation
cell viability
chronic wound
controlled study
cytotoxicity
diabetic wound
drug release
epidermal thickness
erythrocyte
human
human cell
HUVEC cell line
immunofluorescence
in vitro study
in vivo study
inductively coupled plasma atomic emission spectrometry
macrophage
nonhuman
oscillation
pH
protein expression
rat
sol-gel
survival rate
synthesis
tissue regeneration
wound care
wound closure
wound healing
wound tissue
bacterium
diabetes mellitus
pharmacology
Skin
PY - 2023
SP - 821-834
ST - Responsive multifunctional hydrogels emulating the chronic wounds healing
cascade for skin repair
TI - Responsive multifunctional hydrogels emulating the chronic wounds healing
cascade for skin repair
85147090225&doi=10.1016%2fj.jconrel.2023.01.049&partnerID=40&md5=51c45829f67820c65e
e686bb24c83956
VL - 354
ID - 9649
ER -
TY - JOUR
AB - A visible-light-driven heterostructured AgI/g-C3N4 was prepared by a
deposition-precipitation method. The composition, structure, morphology, and
optical properties of the photocatalyst were characterized by Brunauer-Emmett-
Teller method (BET), X-ray powder diffraction (XRD), Fourier transform-infrared
spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron
microscope (TEM), scanning electron microscope (SEM), UV–vis diffused reflectance
spectroscopy (DRS), photoluminescence spectroscopy (PL), photocurrent, and
electrochemical impedance spectroscopy (EIS), respectively. AgI/g-C3N4 composite
photocatalysts exhibited higher photocatalytic activities than those of AgI
nanoparticles and g-C3N4 in the degradation of diclofenac (a model anti-
inflammatory medicine) under visible light irradiation (λ ≥ 400 nm). When the mass
molar ratio of AgI was 45% in AgI/g-C3N4, the reaction rate constant of diclofenac
degradation reached 0.561 min−1, which was almost 12.5 and 43.2 times higher than
that achieved by AgI (0.045 min−1) and g-C3N4 (0.013 min−1). The h+ and O2 - were
pinpointed as the main reactive species in the photocatalytic reaction using their
obligate radical scavengers. Diclofenac was completely degraded and partly
mineralized during the photodegradation. The main intermediates were determined by
liquid chromatograph mass spectrometer (LC-MS), and toxicological assessments were
carried out to evaluate the change of toxicity in the degradation process. In
addition, the photocatalysts showed excellent stability over multiple reaction
cycles. Finally, a possible photocatalytic and charge separation mechanism was
proposed. © 2017 Elsevier Inc.
AN - rayyan-553781716
AU - Zhang, W.
AU - Zhou, L.
AU - Shi, J.
AU - Deng, H.
DO - 10.1016/j.jcis.2017.02.022
KW - AgI
Diclofenac
G-C3N4
Heterojunction
Visible light photocatalysis
Complexation
Electrochemical impedance spectroscopy
Heterojunctions
Light
Optical properties
Photocatalysis
Photocatalysts
Photodegradation
Photoluminescence spectroscopy
Precipitation (chemical)
Rate constants
Semiconductor quantum wells
Silver halides
Spectrometers
diclofenac
graphite
hydroxyl radical
nanocarrier
nanocomposite
nanosheet
organohalogen derivative
oxygen radical
scavenger
silver halide
unclassified drug
Brunauer-Emmett-Teller method
Charge separation mechanism
Deposition precipitation methods
G-C3N4
Visible light photocatalytic activity
Visible-light irradiation
Visible-light photocatalysis
Article
brunauer emmett teller method
chemical structure
constants and coefficients
controlled study
deposition precipitation method
drug degradation
drug stability
electrochemical impedance spectroscopy
human
human cell
investigative procedures
light
MTT assay
multiple reaction monitoring
nanofabrication
nanopharmaceutics
optics
photocatalysis
photodegradation
photoluminescence
priority journal
reaction rate constant
scanning electron microscope
surface area
PY - 2017
SP - 167-176
ST - Fabrication of novel visible-light-driven AgI/g-C3N4 composites with enhanced
visible-light photocatalytic activity for diclofenac degradation
T2 - Journal of Colloid and Interface Science
TI - Fabrication of novel visible-light-driven AgI/g-C3N4 composites with enhanced
visible-light photocatalytic activity for diclofenac degradation
85013224748&doi=10.1016%2fj.jcis.2017.02.022&partnerID=40&md5=cccd4764cd9557783e5a8
b76a279a47f
VL - 496
ID - 9650
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have attracted increased interest and are
currently used in various industries including medicine, cosmetics, textiles,
electronics, and pharmaceuticals, owing to their unique physical and chemical
properties, particularly as antimicrobial and anticancer agents. Recently, several
studies have reported both beneficial and toxic effects of AgNPs on various
prokaryotic and eukaryotic systems. To develop nanoparticles for mediated therapy,
several laboratories have used a variety of cell lines under in vitro conditions to
evaluate the properties, mode of action, differential responses, and mechanisms of
action of AgNPs. In vitro models are simple, cost-effective, rapid, and can be used
to easily assess efficacy and performance. The cytotoxicity, genotoxicity, and
biocompatibility of AgNPs depend on many factors such as size, shape, surface
charge, surface coating, solubility, concentration, surface functionalization,
distribution of particles, mode of entry, mode of action, growth media, exposure
time, and cell type. Cellular responses to AgNPs are different in each cell type
and depend on the physical and chemical nature of AgNPs. This review evaluates
significant contributions to the literature on biological applications of AgNPs. It
begins with an introduction to AgNPs, with particular attention to their overall
impact on cellular effects. The main objective of this review is to elucidate the
reasons for different cell types exhibiting differential responses to nanoparticles
even when they possess similar size, shape, and other parameters. Firstly, we
discuss the cellular effects of AgNPs on a variety of cell lines; Secondly, we
discuss the mechanisms of action of AgNPs in various cellular systems, and try to
elucidate how AgNPs interact with different mammalian cell lines and produce
significant effects; Finally, we discuss the cellular activation of various
signaling molecules in response to AgNPs, and conclude with future perspectives on
research into AgNPs.
AN - rayyan-553781717
AU - Zhang, X. F.
AU - Shen, W.
AU - Gurunathan, S.
DO - 10.3390/ijms17101603
IS - 10
KW - Cell Line
PY - 2016
SN - 1422-0067
ST - Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in
Vitro Model
TI - Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in
Vitro Model
VL - 17
Y2 - 10
ID - 9651
ER -
TY - JOUR
AB - Curcumin is a polyphenolic substance extracted from plants such as Curcuma
longa, Curcuma zedoaria, and radix curcumae, and it has attracted much attention
because of the anti-inflammatory, antioxidant, anti-tumor, anti-bacterial and other
multiple pharmacological effects. Cervical cancer is one of the most common
malignant tumors in women. With the application of HPV (human papillomavirus)
vaccine, the incidence of cervical cancer is expected to be reduced, but it remains
difficult to promote the vaccine among low-income population. As a commonly used
food additive, curcumin has recently been found to have a significant therapeutic
effect in the treatment of cervical cancer. In recent years, numerous in vitro and
in vivo studies have found that curcumin can have significant efficacy in anti-
cervical cancer treatment by promoting apoptosis, inhibiting tumour cell pro-
liferation, metastasis and invasion, inhibiting HPV and inducing autophagy in
tumour cells. However, due to poor water solubility, rapid catabolism, and low
bioavailability of curcumin, studies on curcumin derivatives and novel formulations
are increasing. Curcumin has a wide range of mechanisms of action against cervical
cancer and may become a novel antitumor drug in the future, opening up new ideas
for the research of curcumin in the field of antitumor. There is a lack of
systematic reviews on the mechanism of action of curcumin against cervical cancer.
Therefore, this study is a review of the literature based on the mechanism of
action of curcumin against cervical cancer, with a view to providing reference
information for scientific and clinical practitioners.
AN - rayyan-553781719
AU - Zhang, X. Y.
AU - Zhu, L.
AU - Wang, X. Z.
AU - Zhang, H. R.
AU - Wang, L. Z.
AU - Xia, L.
DO - 10.1016/j.biopha.2023.114590
KW - Uterine Cervical Neoplasms
PY - 2023
SN - 0753-3322 1950-6007
ST - Basic research on curcumin in cervical cancer: Progress and perspectives
TI - Basic research on curcumin in cervical cancer: Progress and perspectives
VL - 162
Y2 - 6
ID - 9653
ER -
TY - JOUR
AB - Neural tissue engineering is a research field aimed at rebuilding
neurological defects resulting from severe trauma, vascular impairment,
syringomyelia, spinal stenosis, malignant and benign tumors, or transverse
myelitis. Of particular interest, neural stem cells (NSCs) and the effective
differentiation and proliferation thereof are attractive research areas that have
yielded widespread utility for implants or neural scaffold materials. Graphene and
its derivatives have more effective and efficient physical, chemical, and
biological abilities than other nanomaterials, and may act as new coating materials
to promote neuronal proliferation and differentiation. Therefore, here, we review
the recent progress of studies that examine the effect of graphenebased materials
on NSCs. We specifically review how graphene and its derivatives influence NSC
adhesion, differentiation, and proliferation. We also discuss the risks of
graphene-based materials, including their anti-inflammatory effects, in the realm
of neural tissue engineering as well as current challenges facing the field today.
AN - rayyan-553781722
AU - Zhang, Y.
AU - Wang, S.
AU - Yang, P.
DO - 10.1155/2020/2519105
PY - 2020
SN - 1687-4110 1687-4129
ST - Effects of Graphene-Based Materials on the Behavior of Neural Stem Cells
T2 - JOURNAL OF NANOMATERIALS
TI - Effects of Graphene-Based Materials on the Behavior of Neural Stem Cells
VL - 2020
Y2 - 7 y3 - 3
ID - 9656
ER -
TY - JOUR
AB - Polymorphonuclear neutrophils (PMN) are one fraction of the major
inflammatory cells in allergic asthma (asthma, in short); the role of PMN in the
asthma pathogenesis is not fully understood yet. This study aims to investigate the
effects of specific Ag-guiding exosomes on suppressing the neutrophil-dominant
airway inflammation. In this study, BALB/c mice were immunized with ovalbumin plus
complete Freund adjuvant to induce an asthma model featured with neutrophil-
dominant lung inflammation. The Ag specific PMN (sPMN)-targeting exosomes (tExo),
that were exosomes carrying a complex of specific Ag/anti-CD64 Ab and Fas ligand,
were constructed to be used to alleviate neutrophilic asthma in mice. We found that
sPMNs were the major cellular component in bronchoalveolar lavage fluid (BALF) in
asthma mice, while less than 3% PMNs in naive control mice. The sPMNs expressed
higher levels of CD64, which formed complexes with Ag-specific IgG (sIgG). The
sIgG/CD64 complex-carrying PMNs could be activated upon exposing to specific Ags.
Exposure to tExos induced Ag-specific PMNs apoptosis. Administration of tExos
efficiently suppressed experimental asthma. We conclude that a fraction of sPMN was
identified in the airway of asthma mice. The sPMNs could be activated upon exposure
to specific Ags. tExos could induce sPMNs apoptosis, that show the translational
potential in the treatment of asthma. © 2020 Elsevier Ltd
AN - rayyan-553781726
AU - Zhao, M. Z.
AU - Li, Y.
AU - Han, H. Y.
AU - Mo, L. H.
AU - Yang, G.
AU - Liu, Z. Q.
AU - Ma, C.
AU - Yang, P. C.
AU - Liu, S.
DO - 10.1016/j.molimm.2020.11.005
KW - Allergy
Asthma
Exosome
Immunotherapy
Neutrophil
Animals
Antibodies
Antigens
Apoptosis
Drug Carriers
Exosomes
Freund's Adjuvant
Hypersensitivity
Immunoglobulin G
Lung
Mice
Mice, Inbred BALB C
Nanoparticles
Neutrophils
Ovalbumin
Pneumonia
Receptors, IgG
Vaccines
CD63 antigen
CD64 antigen
CD81 antigen
CD9 antigen
Fas ligand
Freund adjuvant
gamma interferon
interleukin 13
interleukin 4
interleukin 5
ovalbumin
silver
antibody
antigen
drug carrier
Fc receptor
immunoglobulin G
nanoparticle
vaccine
allergic asthma
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bone marrow derived dendritic cell
controlled study
exosome
immunoprecipitation
immunotherapy
lung parenchyma
mouse
neutrophil
nonhuman
pneumonia
priority journal
animal
asthma
Bagg albino mouse
hypersensitivity
immunology
lung
Vaccination
PY - 2021
SP - 103-111
ST - Specific Ag-guiding nano-vaccines attenuate neutrophil-dominant allergic
asthma
T2 - Molecular Immunology
TI - Specific Ag-guiding nano-vaccines attenuate neutrophil-dominant allergic
asthma
85096590572&doi=10.1016%2fj.molimm.2020.11.005&partnerID=40&md5=0bd35ceee865b4b5578
299c6147b0732
VL - 129
ID - 9660
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) based antibacterial materials are widely applied
to commodity and clinic wound treatments. However, genotoxicity and inflammatory
response induced by AgNPs inhibit their application as the antibacterial coating of
medical devices like catheters. A novel gelatin-AgNPs coating manufacture method
was introduced here to generate an antibacterial coating, which nearly immunes to
inflammatory, on basal PHBV material. The novel gelatin-AgNPs coating was produced
by immobilizing gelatin on the Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)
membrane and subsequently fixing AgNPs on acquired gelatin coating. Prepared
gelatin-AgNPs coatings displayed considerable antibacterial capacity. These
gelatin-AgNPs coatings did not cause inflammation, growth inhibition or apoptosis
to normal human embryonic lung fibroblasts, MRC-5 cells, by analyzing the
transcription levels of relevant genes in these cells incubated with tested
coatings for 4 days. Hence, this novel gelatin-AgNPs coating manufacture method
paved its way to apply in medical devices manufacture including catheters. (C) 2016
Elsevier B.V. All rights reserved.
AN - rayyan-553781727
AU - Zhao, X. X.
AU - Liu, H. R.
AU - Hu, Y. B.
AU - Huang, J. Y.
AU - Zhang, S. H.
AU - Ja, F.
DO - 10.1016/j.reactfunctpolym.2016.07.014
KW - Inflammation
Gelatin
PY - 2016
SN - 1381-5148 1873-166X
SP - 54-59
ST - A novel gelatin-AgNPs coating preparing method for fabrication of
antibacterial and no inflammation inducible coatings on PHBV
T2 - REACTIVE & FUNCTIONAL POLYMERS
TI - A novel gelatin-AgNPs coating preparing method for fabrication of
antibacterial and no inflammation inducible coatings on PHBV
VL - 107
Y2 - 10
ID - 9661
ER -
TY - JOUR
AB - Psoriasis, an autoimmune inflammatory skin disorder, is one of the commonest
immune-mediated disease conditions affecting individuals globally. At the moment,
the conventional methods applied against psoriasis treatment have various drawbacks
involving limited efficacy, skin irritation, immunosuppression, etc. Therefore, it
is important for scientists to find a more potent and alternative drug approach
towards psoriasis therapeutics. Natural medicine still remains an important source
for new drug discovery due to its therapeutical significance in various drug
administration routes. However, the traditional formulation of topical therapies
for psoriasis is limited in efficacy, which limits the use of natural medicine.
Based on the aforementioned limitations, the use of nanocarriers in preparation of
these topical herbal products could be tremendously beneficial in enhancing the
efficacy of topical medications. Growing pieces of evidence have proposed that the
utilization of nanocarriers in transdermal preparation as a prospective technique,
with regards to better potency, directs drug absorption to site of action, and
minimum toxicity effect respectively. In the course of this review, we emphasized
the pathological mechanism of psoriasis, natural medicine formula, active
components of natural medicine, and nanopreparations used in the treatment of
psoriasis. Graphical abstract: [Figure not available: see fulltext.]. © 2021,
Controlled Release Society.
AN - rayyan-553781731
AU - Zhao, Z.
AU - Liu, T.
AU - Zhu, S.
AU - Pi, J.
AU - Guo, P.
AU - Qi, D.
AU - Liu, Z.
AU - Li, N.
DO - 10.1007/s13346-021-01031-3
IS - 6
KW - Nanoparticles
Natural medicine
Psoriasis
Topical drug delivery system
Administration, Cutaneous
Drug Carriers
Humans
Prospective Studies
3 (3,4 dihydroxyphenyl)lactic acid
aurantiin
autoantigen
baicalein
baicalin
capsaicin
celastrol
convallatoxin
Cornus officinalis extract
curcumin
cycloastragenol
cytokine
diosgenin
geraniol
ginsenoside
Glycyrrhiza glabra root
gold nanoparticle
herbaceous agent
interleukin 17
interleukin 19
interleukin 1beta
interleukin 23
interleukin 6
khellactone
liposome
messenger RNA
moutan cortex
nanocarrier
nanoparticle
natural product
niosome
oxymatrine
paclitaxel
psoralen
resveratrol
silver nanoparticle
turmeric
unclassified drug
withanolide
drug carrier
Acorus
Angelica sinensis
Artemisia capillaris
bilayer membrane
capillary endothelial cell
cell cycle arrest
Clematis
Cornus officinalis
cream
cytokine release
drug absorption
drug efficacy
Forsythia suspensa
Fritillaria cirrhosa
fruit
HaCat cell line
herb
herbal medicine
human
immune system
keratinocyte
Lonicera
Mentha
nanomedicine
nonhuman
ointment
pathogenesis
Pogostemon cablin
prescription
psoriasis
Review
Scrophularia
Scutellaria baicalensis
skin irritation
topical treatment
cutaneous drug administration
prospective study
PY - 2022
SP - 1326-1338
ST - Natural medicine combined with nanobased topical delivery systems: a new
strategy to treat psoriasis
T2 - Drug Delivery and Translational Research
TI - Natural medicine combined with nanobased topical delivery systems: a new
strategy to treat psoriasis
85110768736&doi=10.1007%2fs13346-021-01031-
3&partnerID=40&md5=1c080c2b7b781e8ed86a84b9e1f6f87c
VL - 12
ID - 9664
ER -
TY - JOUR
AB - Novel neural interfaces capable of reliably capturing electrical signals are
crucial for the development of prostheses. Longitudinal intrafascicular electrodes
(LIFEs) have been proposed as a promising technology, and their feasibility and
biocompatibility need to be investigated for long-term implantation. In this study,
custom-designed 95%Pt–5%Ir intrafascicular electrodes were implanted into the
sciatic nerves of 14 rabbits using our novel direct microsurgical technique. The
biocompatibility and their ability to record electrophysiological signals were
serially investigated up to 9 months after implantation. Nerve tissues were
examined using light and transmitted electron microscopy, and axon diameters were
quantified, evaluated over time, and compared with sham-control (N = 4). Selective
stimulation and stable recording properties of electrical signals were achieved by
intrafascicular electrodes along the experimental period. While
electrophysiological signal amplitude decreased by as early as 1 month after
implantation (p < 0.05), the signal strength recovered to baseline levels by 3–5
months (p > 0.05). Axon diameter results showed a similar trend of initial decline
(10.8% reduction, p < 0.01) followed by gradual recovery by 6 months (p > 0.05).
Microstructural and ultrastructural analysis revealed modest tissue damage at the
implantation site after implantation with gradual normalization over time.
Intrafascicular electrodes implanted with direct microsurgical techniques
demonstrated good biocompatibility and have great potential for long-term
implantation and electrophysiological recordings. Though subtle tissue damage
impaired ability to capture electrophysiological signals in the first 2 months,
this damage gradually normalized after 3 months, and was fully normalized by 6
months. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater,
107B: 435–444, 2019. © 2018 Wiley Periodicals, Inc.
AN - rayyan-553781732
AU - Zhen, G.
AU - Chen, H.
AU - Tsai, S. Y.
AU - Zhang, J.
AU - Chen, T.
AU - Jia, X.
DO - 10.1002/jbm.b.34135
IS - 2
electrophysiology
intrafascicular electrodes
peripheral nerve
Animals
Electrodes, Implanted
Feasibility Studies
Materials Testing
Rabbits
Sciatic Nerve
Biocompatibility
Electrophysiology
Tissue
ammonia
eosin
hematoxylin
Electrical signal
Electrophysiological recordings
Implantation sites
Neural interfaces
Peripheral nerves
Recording properties
Selective stimulations
Ultrastructural analysis
animal cell
animal experiment
Article
axon
collagen fibril
connective tissue
controlled study
electrode implantation
electron microscopy
electrophysiological procedures
giant cell
image analysis
inflammation
inflammatory cell
information processing
latent period
Leporidae
lymphocyte
microsurgery
myelin sheath
myelinated nerve
nervous tissue
neutrophil
nonhuman
phagocytosis
plasma cell
scar tissue
Schwann cell
sciatic nerve
silver staining
animal
electrode implant
feasibility study
materials testing
metabolism
surgery
Electrodes
PY - 2019
SP - 435-444
ST - Long-term feasibility and biocompatibility of directly microsurgically
implanted intrafascicular electrodes in free roaming rabbits
T2 - Journal of Biomedical Materials Research - Part B Applied Biomaterials
TI - Long-term feasibility and biocompatibility of directly microsurgically
implanted intrafascicular electrodes in free roaming rabbits
85045840647&doi=10.1002%2fjbm.b.34135&partnerID=40&md5=c9d049dd34af69186f87aa6f55b4
3189
VL - 107
ID - 9665
ER -
TY - JOUR
AB - In clinical practice, we noticed that triple negative breast cancer (TNBC)
patients had higher shear-wave elasticity (SWE) stiffness than non-TNBC patients
and a higher α-SMA expression was found in TNBC tissues than the non-TNBC tissues.
Moreover, SWE stiffness also shows a clear correlation to neoadjuvant response
efficiency. To elaborate this phenomenon, TNBC cell membrane-modified polylactide
acid-glycolic acid (PLGA) nanoparticle was fabricated to specifically deliver
artesunate to regulate SWE stiffness through inhibiting CAFs functional status. As
tested in MDA-MB-231 and E0771 orthotopic tumor models, CAFs functional status
inhibited by 231M-ARS@PLGA nanoparticles (231M-AP NPs) had reduced the SWE
stiffness as well as attenuated hypoxia of tumor as tumor soil loosening agent
which amplified the antitumor effects of paclitaxel and PD1 inhibitor. Single-cell
sequencing indicated that the two main CAFs (extracellular matrix and wound healing
CAFs) that produces extracellular matrix could influence the tumor SWE stiffness as
well as the antitumor effect of drugs. Further, biomimetic nanoparticles inhibited
CAFs function could attenuate tumor hypoxia by increasing proportion of
inflammatory blood vessels and oxygen transport capacity. Therefore, our finding is
fundamental for understanding the role of CAFs on affecting SWE stiffness and drugs
antitumor effects, which can be further implied in the potential clinical
theranostic predicting in neoadjuvant therapy efficacy through non-invasive
analyzing of SWE imaging. © 2022 The Authors
AN - rayyan-553781733
AU - Zheng, D.
AU - Zhou, J.
AU - Qian, L.
AU - Liu, X.
AU - Chang, C.
AU - Tang, S.
AU - Zhang, H.
AU - Zhou, S.
DO - 10.1016/j.bioactmat.2022.10.025
KW - Biomimetic nanoparticles
Cancer-associated fibroblasts
Shear-wave elasticity imaging
Theranostic prediction
alpha smooth muscle actin
artesunate
CD3 antigen
CD4 antigen
CD47 antigen
CD8 antigen
discoidin domain receptor 1
galectin 1
gelatinase A
gelatinase B
hypoxia inducible factor 1alpha
Ki 67 antigen
matrix metalloproteinase 14
nanoparticle
paclitaxel
silver nanoparticle
Smad protein
vimentin
angiogenesis
animal model
animal tissue
apoptosis
Article
bioinformatics
blood vessel
cancer associated fibroblast
cancer patient
cancer recurrence
CD8+ T lymphocyte
cell infiltration
cell membrane
cell proliferation
clinical practice
controlled study
cytotoxicity
dispersity
down regulation
drug loading capacity
drug mechanism
echography
endothelium cell
EO771 cell line
female
flow cytometry
follow up
Fourier transform mass spectrometry
functional status
gene expression
gene identification
half life time
human
human cell
human tissue
image analysis
imaging stiffness
immune response
immunofluorescence
lymph node metastasis
metastasis
microarray analysis
microscopy
mouse
multimodal imaging
natural killer cell
neoadjuvant therapy
nonhuman
oxygen transport
particle size
phagocytosis
protein expression
quality control
shear wave elastography
single cell analysis
T-47 cell line
tissue microarray
triple negative breast cancer
tumor associated leukocyte
tumor invasion
tumor volume
tumor weight
tumor xenograft
TUNEL assay
Western blotting
wound healing
Breast Neoplasms
Nanoparticles
PY - 2023
SP - 567-587
ST - Biomimetic nanoparticles drive the mechanism understanding of shear-wave
elasticity stiffness in triple negative breast cancers to predict clinical
treatment
T2 - Bioactive Materials
TI - Biomimetic nanoparticles drive the mechanism understanding of shear-wave
elasticity stiffness in triple negative breast cancers to predict clinical
treatment
85141307167&doi=10.1016%2fj.bioactmat.2022.10.025&partnerID=40&md5=f0365a5f4bfc2c85
8d6d5448f5f099cd
VL - 22
ID - 9666
ER -
TY - JOUR
AB - Increasing utilization of stabilized iron sulfides (FeS) nanoparticles
implies an elevated release of the materials into the environment. To understand
potential impacts and underlying mechanisms of nanoparticle-induced stress, we used
the transcriptome sequencing (RNA-seq) technique to characterize the
transcriptomesfrom adult zebrafish exposed to 10 mg/L carboxymethyl cellulose (CMC)
stabilized FeS nanoparticles for 96 h, demonstrating striking differences in the
gene expression profiles in liver. The exposure caused significant expression
alterations in genes related to immune and inflammatory responses, detoxification,
oxidative stress and DNA damage/repair. The complement and coagulation cascades
Kyoto encyclopedia of genes and genomes (KEGG) pathway was found significantly up-
regulated under nanoparticle exposure. The quantitative real-time polymerase chain
reaction using twelve genes confirmed the RNA-seq results. We identified several
candidate genes commonly regulated in liver, which may serve as gene indicators
when exposed to the nanoparticles. Hepatic inflammation was further confirmed by
histological observation of pyknotic nuclei, and vacuole formation upon exposure.
Tissue accumulation tests showed a 2.2 times higher iron concentration in the fish
tissue upon exposure. This study provides preliminary mechanistic insights into
potential toxic effects of organic matter stabilized FeS nanoparticles, which will
improve our understanding of the genotoxicity caused by stabilized nanoparticles.
AN - rayyan-553781734
AU - Zheng, M.
AU - Lu, J. G.
AU - Zhao, D. Y.
DO - 10.1038/s41598-018-26499-x
KW - Transcriptome
Sulfides
Cellulose
Oxalic Acid
Norisoprenoids
Iron
PY - 2018
SN - 2045-2322
ST - Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish
in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide
Nanoparticles
TI - Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish
in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide
Nanoparticles
VL - 8
Y2 - 5 y3 - 24
ID - 9667
ER -
TY - JOUR
AB - Rhei Radix et Rhizoma, also known as rhubarb or Da Huang, has been widely
used as a spice and as traditional herbal medicine for centuries, and is currently
marketed in China as the principal herbs in various prescriptions, such as Da-
Huang-Zhe-Chong pills and Da-Huang-Qing-Wei pills. Emodin, a major bioactive
anthraquinone derivative extracted from rhubarb, represents multiple health
benefits in the treatment of a host of diseases, such as immune-inflammatory
abnormality, tumor progression, bacterial or viral infections, and metabolic
syndrome. Emerging evidence has made great strides in clarifying the multi-
targeting therapeutic mechanisms underlying the efficacious therapeutic potential
of emodin, including anti-inflammatory, immunomodulatory, anti-fibrosis, anti-
tumor, anti-viral, anti-bacterial, and anti-diabetic properties. This comprehensive
review aims to provide an updated summary of recent developments on these
pharmacological efficacies and molecular mechanisms of emodin, with a focus on the
underlying molecular targets and signaling networks. We also reviewed recent
attempts to improve the pharmacokinetic properties and biological activities of
emodin by structural modification and novel material-based targeted delivery. In
conclusion, emodin still has great potential to become promising therapeutic
options to immune and inflammation abnormality, organ fibrosis, common malignancy,
pathogenic bacteria or virus infections, and endocrine disease or disorder.
Scientifically addressing concerns regarding the poor bioavailability and vague
molecular targets would significantly contribute to the widespread acceptance of
rhubarb not only as a dietary supplement in food flavorings and colorings but also
as a health-promoting TCM in the coming years. © 2021, The Author(s).
AN - rayyan-553781735
AU - Zheng, Q.
AU - Li, S.
AU - Li, X.
AU - Liu, R.
DO - 10.1186/s13020-021-00509-z
IS - 1
KW - Emodin
Modification
Pharmacokinetics
Pharmacology
Toxicology
11beta hydroxysteroid dehydrogenase 1
adenylate kinase
anthraquinone derivative
caspase 3
cremophor
cryopyrin
daunorubicin
decitabine
emodin
flavoring agent
fluorouracil
gamma interferon
glucose
I kappa B kinase alpha
interleukin 4
interleukin 6
liposome
macrogol 400
nicotinamide
paclitaxel
peroxisome proliferator activated receptor gamma
poloxamer
polyethyleneimine
polymer
polyvinylsulfonate
protein Bax
purinergic P2X7 receptor
rhubarb extract
scleroprotein
silver nanoparticle
sorafenib
transcription factor RelA
unclassified drug
acute lung injury
acute pancreatitis
antifibrotic activity
antiviral activity
bacterial infection
cataract
Chinese medicine
Coxsackievirus B3
cyclic voltammetry
density functional theory
diabetes mellitus
diabetic neuropathy
dietary supplement
drug efficacy
drug elimination
drug mechanism
drug potentiation
drug solubility
drug targeting
Enterovirus A71
fatty liver
food color
health promotion
human
immunomodulation
immunopathology
inflammation
Influenza A virus
liver cell carcinoma
liver toxicity
malignant neoplasm
metastasis inhibition
molecular mechanics
nephrotoxicity
nonalcoholic fatty liver
nonhuman
protein expression
Review
rhubarb
rotating disk electrode voltammetry
signal transduction
Streptococcus suis
virus infection
X ray crystallography
PY - 2021
ST - Advances in the study of emodin: an update on pharmacological properties and
mechanistic basis
T2 - Chinese Medicine (United Kingdom)
TI - Advances in the study of emodin: an update on pharmacological properties and
mechanistic basis
85116724721&doi=10.1186%2fs13020-021-00509-
z&partnerID=40&md5=1b9ba87a7316efa6a42e53ea3002dd6f
VL - 16
ID - 9668
ER -
TY - JOUR
AB - BACKGROUND: Nanosilver has significant antibacterial properties, and
nanohydroxyapatite has good biological activity and mechanical strength, while
their mixture cannot only promote bone formation but also have antibacterial
properties. OBJECTIVE: To observe the effect of nanosilver and nanohydroxyapatite
mixed filling on the osseointegration of immediate implants. METHODS: Nine New
Zealand white rabbits were randomly divided into experimental group (n=6) and
control group (n=3) after four incisors from the upper and lower jaw were
extracted. The mixture of nanosilver and nanohydroxyapatite was filled into the
tooth socket in the experimental group, while nanohydroxyapatite was filled into
the tooth socket in the control group. Titanium screw was immediately implanted
into both groups. The intact maxilla and mandibular specimens were harvested at the
4th, 8th, and 12th weeks after operation. Gross observation, X-ray bone density
analysis, torque test and histological observation were conducted. RESULTS AND
CONCLUSION: The gray value and maximum torque value of regenerated osseous tissue
at different time points in experimental group were significantly higher than those
in the control group (P < 0.05). Within 12 weeks of implantation, the bone
formation rate and maturity of new bone tissue were higher in the experimental
group compared with the control group, and no inflammatory cell infiltration
occurred. At the 4th week after implantation, there was a large amount of
inflammatory cell infiltration, and few inflammatory existed at the 8th week after
implantation. These results demonstrate that compared with nanohydroxyapatite
alone, the mixture of nanosilver and nanohydroxyapatite shows better antibacterial
effect, biocompatibility and osteoinductive ability, which may accelerate
osseointegration and promote osteogenesis. © 2015, Journal of Clinical
Rehabilitative Tissue Engineering Research. All rights reserved.
AN - rayyan-553781736
AU - Zheng, Y.
AU - Li, B. T.
AU - Wang, L. J.
AU - Fan, S. F.
AU - Hou, X. W.
DO - 10.3969/j.issn.2095-4344.2015.47.019
IS - 47
KW - Durapatite
Nanostructures
Osseointegration
Tissue Engineering
hydroxyapatite
silver nanoparticle
titanium
animal cell
animal experiment
animal tissue
Article
biocompatibility
bone density
bone development
bone implant
bone regeneration
bone tissue
cell infiltration
controlled study
experimental rabbit
histology
incisor
inflammatory cell
mandible
maxilla
nonhuman
oral screw
ossification
tooth extraction
tooth socket
torque
PY - 2015
SP - 7649-7654
ST - Effect of nanosilver and nanohydroxyapatite mixed filling on osseointegration
of immediate implants
T2 - Chinese Journal of Tissue Engineering Research
TI - Effect of nanosilver and nanohydroxyapatite mixed filling on osseointegration
of immediate implants
85018791447&doi=10.3969%2fj.issn.2095-
4344.2015.47.019&partnerID=40&md5=e44a0c1c91f9d73f4656622a6cac565d
VL - 19
ID - 9669
ER -
TY - JOUR
AB - Objective. To study bioinertness of new nanosized carbon-containing in
compounds in experiment. Methods. The pulsed vacuum arc method was used to deposite
the coating of nitinol wire. Nitinol wire stents were implanted in the parenchymal
organs (kidney and liver) and in the choledoch of sexually mature Wistar rats. The
animals were taken out of the experiment on the 14th and 30th day after
implantation. The thickness of developing reactive tissue areas was measured; the
cytoarchitectonics around the implants was evaluated. Results. In the cases of
implantation in the parenchymal organs the quantitative and qualitative
characteristics depended on the implantation site have been defined. Inflammatory
changes around implants in the liver proved to be 1,5-2 fold more intensive than in
the kidneys the same period of observation. Qualitative analysis of
cytoarchitectonics around the implants in different groups has not revealed
statistically significant differences depending on the implant material. The
quantitative assessment of inflammatory infiltration in the group with
nanostructured coating based on amorphous carbon matrix with including of silver
nanoscale clusters was less intense regardless of the implantation site.
Implantation of new carbon nanoscale materials into the rat choledoch lumen has not
followed by necrobiotic changes and perforation of its wall; no mortality was
observed in the study groups. The reaction of the extrahepatic biliary tract to the
introduction of implant such homologous when it implanted in parenchymal organs.
Conclusion. The materials being developed for implantation showed no cytotoxicity.
The best indicators of bioinertness were registered in the group of implants with
nanostructural coating based on amorphous carbon matrix with inclusions of
nanoscale clusters of silver. This result can be explained by the inertia of the
carbon to the tissues and by antiproliferative properties of silver.
AN - rayyan-553781737
AU - Zhernakova, N. I.
AU - Dolzhikov, A. A.
AU - Bocharova, K. A.
AU - Kolpakov, A. J.
AU - Dmitriem, V. N.
DO - 10.18484/2305-0047.2015.5.491
IS - 5
KW - Antiproliferative properties of silver
Biomedical materials
Cytotoxicity
Implant
Inflammation
Pulsed vacuum arc method
Stent
PY - 2015
SP - 491-499
ST - Assessment of cytotoxicity of carbon-containing nanosized coatings
T2 - Novosti Khirurgii
TI - Assessment of cytotoxicity of carbon-containing nanosized coatings
84949425844&doi=10.18484%2f2305-
0047.2015.5.491&partnerID=40&md5=db95909544eda5824b48a597cbaedeab
VL - 23
ID - 9670
ER -
TY - JOUR
AB - The study investigates cytokines secretion ability of leukocytic cells
stimulated by materials contained in implants. The stimulated cytokine-production
activity in relation to the cytokines (IFNα, IFNγ, TNFα, IL-1ß, IL-1βRa, IL-2, IL-
6, IL-8) was changed in the presence of and depended on the type of the studied
implant. The most intensive inflammatory reaction was observed in case with metals
and polyurethane, the least intensive one was registered in the presence of a
nanostructural coating based on amorphous carbon and silver nanoparticles (NPs:Ag).
AN - rayyan-553781738
AU - Zhernakova, N. I.
AU - Dolzhykov, A. A.
AU - Bocharova, K. A.
AU - Dmitriyev, V. N.
AU - Kolpakov, A. Y.
AU - Manokhin, S. S.
AU - Miroshnichenko, O. V.
AU - Liubushkin, A. V.
IS - 6
KW - Cytokines
Inflammation
Medical implant
alpha interferon
gamma interferon
interleukin 1beta
interleukin 2
interleukin 6
nanocoating
silver nanoparticle
Article
biocompatibility
cell stimulation
controlled study
cytokine production
cytokine release
in vitro study
leukocyte
nanoanalysis
roentgen spectroscopy
surface property
PY - 2014
SP - 1477-1480
ST - Study of nanostructural coating biocompatibility in-vitro
TI - Study of nanostructural coating biocompatibility in-vitro
84911064921&partnerID=40&md5=81c45dad7ff361930e0d9009fd269bb6
VL - 5
ID - 9671
ER -
TY - JOUR
AB - Background: Voriconazole is the traditionally used antifungal agent, but its
ophthalmic form is unsatisfactory. A novel ophthalmic drug delivery system with
biomedical devices may be of promising for the prognosis of fungal keratitis.
Objective: This study was to investigate the sustained release, therapeutic effect
and biocompatibility of effect and quaternized chitosan functionalized with
carboxylated graphene and nano-silver and voriconazole (CS-ETA/Ag/GO/Vor) for
fungal keratitis. Methods: This study complied with the Regulations for the
Administration of Affair Concerning Experimental Animals of State Science and
Technology Commission. Two hundred and ten SPF female C57BL/6 mice were selected
with the age 6-8 weeks for the biocompatibility experiment (30 mice) and
therapeutic observation of CS-ETA/Ag/GO/Vor (180 mice). CS-ETA/Ag/GO and
CS-ETA/Ag/GO/Vor were attached on the normal corneas of mice and compared with the
normal mice to assess the histopathological changes. Aspergillus fumigatus-infected
mouse models were established in the left eyes of 180 mice by intrastromally
injection of 2.0 μL Aspergillus fumigatus suspension with the density of 5 × 107
CFU/ml, then the mice were randomized into the model control group, CS-ETA/Ag/GO
group and CS-ETA/Ag/GO/Vor group, and the corresponding membrane were attached the
central corneas in different groups. In 1 day, 3, 5, 7 days after modeling, the
corneas were examined under the slit lamp microscope and scored, and corneal
sections were prepared for the histopathological examination. Fungal activity was
confirmed by plate counts, and real-time PCR was employed to assay the relative
expressions of interleukin-1β (IL-1β) mRNA and tumor necrosis factor-α (TNF-α) mRNA
in the corneas. Results: No morphological abnormality was seen in the corneas in
the normal control group, CS-ETA/Ag/GO group and CS-ETA/Ag/GO/Vor group. Corneal
inflammatory score was significantly lower in the CS-ETA/Ag/GO/Vor group in various
time points, with a significant differences among the groups and time points
(Fgroup=237.29, P=0.00; Ftime=260.33, P=0.00). The edema, necrosis or perforation
of cornea were seen in the model control group, and slighter inflammatory response
in the CS-ETA/Ag/GO group, and corneal edema was gradually disappear in the CS-
ETA/Ag/GO/Vor group. The corneal fungal loads were highest in the model control
group and lowest in the CS-ETA/Ag/GO/ Vor group, with significant differences among
the three groups and various time points (Fgroup=113.15, P=0.00; Ftime=126.52,
P=0.00). The relative expressions of IL-1β mRNA and TNF-α mRNA in the corneas
peaked in the fifth day after modeling in all of the three groups, and the
expression levels of IL-1β mRNA and TNF-α mRNA in the corneas were lowest in the
CS-ETA/Ag/GO/Vor group, showing significant differences among the groups and time
points (IL-1β: Fgroup=189.90, P=0.00; Ftime=108.56, P=0.00; TNF-α: Fgroup=82.55,
P=0.00; Ftime=44.36, P=0.00). Conclusions: CS-ETA/Ag/GO/Vor delivery system plays
an anti-fungal activity in fungal keratitis by the synergistic effect of
voriconazole and Ag+. In addition, CS-ETA/Ag/GO/Vor appears to have a good safety
after topical application. Copyright © 2015 by the Chinese Medical Association.
AN - rayyan-553781739
AU - Zhong, J.
AU - Yuan, J.
AU - Jiang, G.
AU - Chen, G.
AU - Sun, Y.
AU - Chen, L.
AU - Deng, Y.
DO - 10.3760/cma.j.issn.2095-0160.2015.05.006
IS - 5
KW - Antifungal agents/therapeutic use
Aspergillus fumigatus
Keratitis
Mice, inbred C57BL
Nanomedicine
Silver/pharmacology
Voriconazole
chitosan
graphene
interleukin 1beta
messenger RNA
silver nanoparticle
voriconazole
animal experiment
animal model
animal tissue
Article
aspergillosis
biocompatibility
carboxylation
controlled study
cornea
cornea edema
cornea necrosis
cornea perforation
drug efficacy
drug safety
female
histopathology
keratitis
keratomycosis
mouse
nonhuman
plate count
protein expression
slit lamp
Aspergillus
PY - 2015
SP - 412-418
ST - Therapeutic efficacy and safety of nano-silver membrane with voriconazole for
Aspergillus fumigatus keratitis after topical application
T2 - Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology
TI - Therapeutic efficacy and safety of nano-silver membrane with voriconazole for
Aspergillus fumigatus keratitis after topical application
84930067376&doi=10.3760%2fcma.j.issn.2095-
0160.2015.05.006&partnerID=40&md5=69006484d18c01f580103dd335785746
VL - 33
ID - 9672
ER -
TY - JOUR
AB - The damaging effects of nanoparticles were hypothesized to be the oxidative
stress caused by the formation of reactive oxygen species and initiation of
inflammatory reactions. In this context a study on the effects of nanosilver
particles on the formation of reactive oxygen species in human lymphocyte culture
was carried out. The obtained results showed that fluorescence intensity
considerably increased after cells had interacted with nanosilver particles of
varying concentrations, indicating the formation of reactive oxygen species and
their accumulation in lymphocyte cells. Morphological study of the lymphocyte cells
under the effects of nanosilver particles showed that the change in morphology
depends on the concentration and size of nanosilver particles: for a size ≤20 nm
the lymphocyte cell significantly shrank with pronounced differences in the
morphological structure of the cell membrane, but for a size ≥200 nm no change was
observed. © 2015 Vietnam Academy of Science & Technology
AN - rayyan-553781740
AU - Zhornik, A.
AU - Baranova, L.
AU - Volotovski, I.
AU - Chizhik, S.
AU - Drozd, E.
AU - Sudas, M.
AU - Ngo, Q. B.
AU - Nguyen, H. C.
AU - Huynh, T. H.
AU - Dao, T. H.
DO - 10.1088/2043-6262/6/2/025003
IS - 2
KW - Lymphocyte
Nanosilver particle
Oxidative stress
Toxicity
Cell culture
Oxygen
Damaging effects
Fluorescence intensities
Human lymphocytes
Morphological structures
Morphological study
Nano silver
Lymphocytes
Humanities
Humanism
Humans
PY - 2015
ST - Interaction of nanosilver particles with human lymphocyte cells
T2 - Advances in Natural Sciences: Nanoscience and Nanotechnology
TI - Interaction of nanosilver particles with human lymphocyte cells
84922310912&doi=10.1088%2f2043-
6262%2f6%2f2%2f025003&partnerID=40&md5=96f1fae9f62686d9af69645e5d308e1b
VL - 6
ID - 9673
ER -
TY - JOUR
AB - Background: Nanoparticles (NPs) entering the biological environment could
interact with biomolecules, but little is known about the interaction between
unsaturated fatty acids (UFA) and NPs. Methods: This study used alpha-linolenic
acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for
hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. Results:
The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis,
fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an
interaction between BSA or LNA and NPs. Exposure to ZnO NPs with the presence of
BSA significantly induced the damage to mitochondria and lysosomes in HepG2 cells,
associated with an increase of intracellular Zn ions, but not intracellular
superoxide. Paradoxically, the release of inflammatory cytokine interleukin-6 (IL-
6) was decreased, which indicated the anti-inflammatory effects of ZnO NPs when BSA
was present. The presence of LNA did not significantly affect all of these
endpoints in HepG2 cells exposed to ZnO NPs and BSA. Conclusions: the results from
the present study indicated that BSA-complexed LNA might modestly interact with ZnO
NPs, but did not significantly affect ZnO NPs and BSA-induced biological effects in
HepG2 cells.
AN - rayyan-553781745
AU - Zhou, Y. W.
AU - Fang, X.
AU - Gong, Y.
AU - Xiao, A. P.
AU - Xie, Y. X.
AU - Liu, L. L.
AU - Cao, Y.
DO - 10.3390/nano7040091
IS - 4
KW - alpha-Linolenic Acid
PY - 2017
SN - 2079-4991
ST - The Interactions between ZnO Nanoparticles (NPs) and alpha-Linolenic Acid
(LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells
T2 - NANOMATERIALS
TI - The Interactions between ZnO Nanoparticles (NPs) and alpha-Linolenic Acid
(LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells
VL - 7
Y2 - 4
ID - 9678
ER -
TY - JOUR
AB - Neuroinflammation, an inflammatory response within the central nervous system
(CNS), is a main hallmark of common neurodegenerative diseases, including
Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral
sclerosis (ALS), among others. The over-activated microglia release pro-
inflammatory cytokines, which induces neuronal death and accelerates
neurodegeneration. Therefore, inhibition of microglia over-activation and
microglia-mediated neuroinflammation has been a promising strategy for the
treatment of neurodegenerative diseases. Many drugs have shown promising
therapeutic effects on microglia and inflammation. However, the blood–brain barrier
(BBB)—a natural barrier preventing brain tissue from contact with harmful plasma
components—seriously hinders drug delivery to the microglial cells in CNS. As an
emerging useful therapeutic tool in CNS-related diseases, nanoparticles (NPs) have
been widely applied in biomedical fields for use in diagnosis, biosensing and drug
delivery. Recently, many NPs have been reported to be useful vehicles for anti-
inflammatory drugs across the BBB to inhibit the over-activation of microglia and
neuroinflammation. Therefore, NPs with good biodegradability and biocompatibility
have the potential to be developed as an effective and minimally invasive carrier
to help other drugs cross the BBB or as a therapeutic agent for the treatment of
neuroinflammation-mediated neurodegenerative diseases. In this review, we
summarized various nanoparticles applied in CNS, and their mechanisms and effects
in the modulation of inflammation responses in neurodegenerative diseases,
providing insights and suggestions for the use of NPs in the treatment of
neuroinflammation-related neurodegenerative diseases. © Copyright © 2021 Zhu, Hu,
Yu, Zhou, Wu, Tang, Qin, Fan and Wu.
AN - rayyan-553781746
AU - Zhu, F. D.
AU - Hu, Y. J.
AU - Yu, L.
AU - Zhou, X. G.
AU - Wu, J. M.
AU - Tang, Y.
AU - Qin, D. L.
AU - Fan, Q. Z.
AU - Wu, A. G.
DO - 10.3389/fphar.2021.683935
KW - blood-brain barrier
central neural system
nanoparticles
neurodegenerative diseases
neuroinflammation
aluminum oxide nanoparticle
arginase 1
cobalt nanoparticle
dendrimer
gold nanoparticle
graphene quantum dot
interleukin 10
interleukin 12
interleukin 1beta
interleukin 23
interleukin 6
liposome
manganese oxide nanoparticle
nanogel
nanoparticle
silica nanoparticle
silver nanoparticle
unclassified drug
Alzheimer disease
astrocytosis
attention disturbance
diffuse Lewy body disease
frontotemporal dementia
human
Huntington chorea
memory disorder
nanoemulsion
neuroprotection
neurotoxicity
nonhuman
Parkinson disease
Review
Inflammation
PY - 2021
ST - Nanoparticles: A Hope for the Treatment of Inflammation in CNS
T2 - Frontiers in Pharmacology
TI - Nanoparticles: A Hope for the Treatment of Inflammation in CNS
85107521954&doi=10.3389%2ffphar.2021.683935&partnerID=40&md5=9a316a9311f82b634fba5f
08ce89afc8
VL - 12
ID - 9679
ER -
TY - JOUR
AB - Nanomedicine is seen as a potential central player in the delivery of
personalized medicine. Biocompatibility issues of nanoparticles have largely been
resolved over the past decade. Despite their tremendous progress, less than 1% of
applied nanosystems can hit their intended target location, such as a solid tumor,
and this remains an obstacle to their full ability and potential with a high
translational value. Therefore, achieving immune-tolerable, blood-compatible, and
biofriendly nanoparticles remains an unmet need. The translational success of
nanoformulations from bench to bedside involves a thorough assessment of their
design, compatibility beyond cytotoxicity such as immune toxicity, blood
compatibility, and immune-mediated destruction/rejection/clearance profile. Here,
we report a one-pot process-engineered synthesis of ultrasmall gold nanoparticles
(uGNPs) suitable for better body and renal clearance delivery of their payloads. We
have obtained uGNP sizes of as low as 3 nm and have engineered the synthesis to
allow them to be accurately sized (almost nanometer by nanometer). The synthesized
uGNPs are biocompatible and can easily be functionalized to carry drugs, peptides,
antibodies, and other therapeutic molecules. We have performed in vitro cell
viability assays, immunotoxicity assays, inflammatory cytokine analysis, a
complement activation study, and blood coagulation studies with the uGNPs to
confirm their safety. These can help to set up a long-term safety-benefit framework
of experimentation to reveal whether any designed nanoparticles are immune-
tolerable and can be used as payload carriers for next-generation vaccines,
chemotherapeutic drugs, and theranostic agents with better body clearance ability
and deep tissue penetration.
AN - rayyan-553781747
AU - Zhu, G. H.
AU - Azharuddin, M.
AU - Islam, R.
AU - Rahmoune, H.
AU - Deb, S.
AU - Kanji, U.
AU - Das, J.
AU - Osterrieth, J.
AU - Aulakh, P.
AU - Ibrahim-Hashi, H.
AU - Manchanda, R.
AU - Nilsson, P. H.
AU - Mollnes, T. E.
AU - Bhattacharyya, M.
AU - Islam, M. M.
AU - Hinkula, J.
AU - Slater, N. K. H.
AU - Patra, H. K.
DO - 10.1021/acsami.1c02834
IS - 20
PY - 2021
SN - 1944-8244 1944-8252
SP - 23410-23422
ST - Innate Immune Invisible Ultrasmall Gold Nanoparticles-Framework for Synthesis
and Evaluation
TI - Innate Immune Invisible Ultrasmall Gold Nanoparticles-Framework for Synthesis
and Evaluation
VL - 13
Y2 - 5 y3 - 26
ID - 9680
ER -
TY - JOUR
AB - Dental caries is a bacteria-caused condition classified among the most common
chronic diseases worldwide. Treatment of dental caries implies the use of materials
having regenerative and anti-bacterial properties, and controlling inflammation is
critical for successful endodontic regeneration. OBJECTIVES: The aim of this study
was to fabricate and characterize a novel composite incorporating sol-gel derived
silver-doped bioactive glass (BG) in a chitosan (CS) hydrogel at a 1:1 wt ratio(Ag-
BG/CS). METHODS: The effect of Ag-BG/CS on dental pulp cells (DPCs) proliferation
was analyzed by CCK-8 assay, whereas the adhesion of DPCs was evaluated by confocal
microscopy. The physical morphology of Ag-BG/CS was analyzed by scanning electron
microscope. The anti-inflammatory effect of Ag-BG/CS was investigated by
quantitative polymerase chain reaction (qPCR). Moreover, the effect of Ag-BG/CS on
odontogenic differentiation of DPCs was studied by immunochemical staining, tissue-
nonspecific alkaline phosphatase staining, qPCR, and western blot analyses. The
antibacterial activity against dental caries key pathogenic bacteria was also
evaluated. RESULTS: The results of this study showed that Ag-BG/CS did not affect
the proliferation of DPCs, it down-regulated the inflammatory-associated markers
(IL-1β, IL-6, IL-8, TNF-α) of DPCs treated with Escherichia coli lipopolysaccharide
(LPS) by inhibiting NF-κB pathway, and enhanced the in vitro odontogenic
differentiation potential of DPCs. Furthermore, Ag-BG/CS strongly inhibited
Streptococcus mutans and Lactobacillus casei growth. CONCLUSIONS: This novel
biomaterial possessed antibacterial and anti-inflammatory activity, also enhanced
the odontogenic differentiation potential of LPS-induced inflammatory-reacted
dental pulp cells. The material introduced in this study may thus represent a
suitable dental pulp-capping material for future clinical applications.
AN - rayyan-553782088
AU - Zhu, N.
AU - Chatzistavrou, X.
AU - Ge, L.
AU - Qin, M.
AU - Papagerakis, P.
AU - Wang, Y.
DO - 10.1016/j.jdent.2019.01.017
J2 - J Dent
Cells, Cultured
*Dental Caries
*Dental Pulp
*Glass
Humans
Odontogenesis
Dental Pulp
LA - eng
N1 - Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking
University, #22 Zhongguancun Nandajie, Haidian District, Beijing, 100081, China.
Electronic address: zhuningxin6221@163.com.; Michigan State University, Department
of Chemical Engineering and Materials Science, East Lansing 48824, MI, USA.
Electronic address: chatzist@egr.msu.edu.; Department of Pediatric Dentistry,
School and Hospital of Stomatology, Peking University, #22 Zhongguancun Nandajie,
Haidian District, Beijing, 100081, China. Electronic address: gelh0919@126.com.;
Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking
University, #22 Zhongguancun Nandajie, Haidian District, Beijing, 100081, China.
Electronic address: qin-man@foxmail.com.; College of Dentistry and Biomedical
Engineering, Toxicology, Pharmacy/Nutrition, Anatomy and Cell Biology Colleges
Graduate Programs, University of Saskatchewan, Canada. Electronic address:
petros.papagerakis@usask.ca.; Department of Pediatric Dentistry, School and
Hospital of Stomatology, Peking University, #22 Zhongguancun Nandajie, Haidian
District, Beijing, 100081, China. Electronic address: cwyyd@126.com.
PY - 2019
SP - 18-26
ST - Biological properties of modified bioactive glass on dental pulp cells
T2 - Journal of dentistry
TI - Biological properties of modified bioactive glass on dental pulp cells
VL - 83
Y2 - 4
ID - 9999
ER -
TY - JOUR
AB - Due to their antibacterial, antifungal, antiviral, and anti-inflammatory
properties, silver and, in recent years, nanosilver (NS) have been widely used in
various fields of human activity. However, it has been found that nanomaterials
acquire new properties, including those with respect to toxicity. The purpose of
this work was to study the effect of NS and the ionic form of silver, silver
sulfate (SS), on somatic mice cells in vivo. A model that is closest to the
conditions of exposure to humans, namely the supply of NS and SS with drinking
water, is used. The effect of NS particles coated with gum Arabic (diameter 14 ±
0.3 nm) and SS at concentrations of 0.1, 5, 50, and 500 mg/L upon 2-week exposure
is studied. A cytom assay, including counting the micronuclei and other nuclear
anomalies in the cells of the bone marrow, lung, colon, and bladder, was conducted.
No effect of NS or SS on bone-marrow cells is revealed in the standard micronucleus
test. NS at a concentration of 50 mg/L increases the cytogenetic effect by 1.9
times at the place of action, the colon, when compared to the control. In the
lungs, the rate of cells with micronuclei is increased threefold under the action
of NS at a concentration of 500 mg/L. The effect of NS on reducing the
proliferation level in the colon is confirmed in vivo; this effect has been
previously found in vitro by other authors. SS at a concentration of 50 mg/L
increases the rate of cells with cytogenetic lesions in the colon and bladder by
1.9 and 1.3 times, respectively. These effects should be considered when assessing
the risk of these compounds. © 2017, Pleiades Publishing, Ltd.
AN - rayyan-553781748
AU - Zhurkov, V. S.
AU - Savostikova, O. N.
AU - Yurchenko, V. V.
AU - Krivtsova, E. K.
AU - Kovalenko, M. A.
AU - Murav’eva, L. V.
AU - Alekseeva, A. V.
AU - Belyaeva, N. N.
AU - Mikhailova, R. I.
AU - Sycheva, L. P.
DO - 10.1134/S1995078017060143
IS - 11
KW - Bone
Cells
Cytology
Cytotoxicity
Mammals
Potable water
Risk assessment
Sulfur compounds
Anti-inflammatories
Bone marrow cells
Cytogenetic effects
Cytotoxic effects
Human activities
Ionic forms
Micronucleus test
Silver sulfate
Silver compounds
Mice
PY - 2017
SP - 667-672
ST - Features of the Mutagenic and Cytotoxic Effects of Nanosilver and Silver
Sulfate in Mice
T2 - Nanotechnologies in Russia
TI - Features of the Mutagenic and Cytotoxic Effects of Nanosilver and Silver
Sulfate in Mice
85044779491&doi=10.1134%2fS1995078017060143&partnerID=40&md5=e169fd12bbbf96723a7535
10b45b8ccf
VL - 12
ID - 9681
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as
well as to agglomerate, which reduces the effectiveness of their use for the
antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake
structure, can be a carrier of AgNP that stabilizes their movement without
inhibiting their antibacterial properties. Considering that the human skin is often
the first contact with antimicrobial agent, the aim of the study was to assess
whether the application of the complex of AgNP and GO is biocompatible with the
skin model in in vitro studies. The conducted tests were performed in accordance
with the criteria set in OECD TG439. AgNP-GO complex did not influence the
genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the
pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon
gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well
as MIP1 beta (macrophage inflammatory protein 1 beta) expressed in the GO group.
Moreover, it showed a positive effect on the micro- and ultra-structure of the skin
model. In conclusion, the synergistic effect of AgNP and GO as a complex can
activate the process of epidermis renewal, which makes it suitable for use as a
material for skin contact.
AN - rayyan-553781750
AU - Zielinska-Gorska, M.
AU - Sawosz, E.
AU - Sosnowska, M.
AU - Hotowy, A.
AU - Grodzik, M.
AU - Gorski, K.
AU - Strojny-Cieslak, B.
AU - Wierzbicki, M.
AU - Chwalibog, A.
IS - 7
KW - Humanities
Humanism
Humans
Epidermis
PY - 2022
SN - 1999-4923
ST - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
Oxide to Human Skin in a 3D Epidermis In Vitro Model
T2 - PHARMACEUTICS
TI - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
VL - 14
Y2 - 7
ID - 9683
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as
well as to agglomerate, which reduces the effectiveness of their use for the
antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake
structure, can be a carrier of AgNP that stabilizes their movement without
inhibiting their antibacterial properties. Considering that the human skin is often
the first contact with antimicrobial agent, the aim of the study was to assess
whether the application of the complex of AgNP and GO is biocompatible with the
skin model in in vitro studies. The conducted tests were performed in accordance
with the criteria set in OECD TG439. AgNP-GO complex did not influence the
genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the
pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon
gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well
as MIP1β (macrophage inflammatory protein 1β) expressed in the GO group. Moreover,
it showed a positive effect on the micro- and ultra-structure of the skin model. In
conclusion, the synergistic effect of AgNP and GO as a complex can activate the
process of epidermis renewal, which makes it suitable for use as a material for
skin contact.
AN - rayyan-553781877
AU - Zielińska-Górska, M.
AU - Sawosz, E.
AU - Sosnowska, M.
AU - Hotowy, A.
AU - Grodzik, M.
AU - Górski, K.
AU - Strojny-Cieślak, B.
AU - Wierzbicki, M.
AU - Chwalibog, A.
IS - 7
J2 - Pharmaceutics
KW - Humanities
Humanism
Humans
Epidermis
LA - eng
N1 - Department of Nanobiotechnology, Institute of Biology, Warsaw University of
Life Sciences, 02-786 Warsaw, Poland.; Department of Nanobiotechnology, Institute
of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, Poland.; Department
of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-
786 Warsaw, Poland.; Department of Nanobiotechnology, Institute of Biology, Warsaw
University of Life Sciences, 02-786 Warsaw, Poland.; Department of
Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786
Warsaw, Poland.; Interdisciplinary Division for Energy Analyses, National Centre
for Nuclear Research, 05-400 Otwock, Poland.; Department of Nanobiotechnology,
Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, Poland.;
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life
Sciences, 02-786 Warsaw, Poland.; Department of Veterinary and Animal Sciences,
University of Copenhagen, 1870 Frederiksberg, Denmark.
PY - 2022
SN - 1999-4923 (Print)
ST - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
T2 - Pharmaceutics
TI - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
VL - 14
Y2 - 7 y3 - 1
ID - 9801
ER -
TY - JOUR
AB - CNS-localized inflammation with microglial activation and macrophage
infiltration contributes to the pathogenesis of a broad spectrum of neurologic
diseases. A direct injection of lipopolysaccharide (LPS) into the striatum of
gerbils induced lectin-positive macrophage parenchymal invasion, minimal local
microglial staining but extensive neurodegeneration (cresyl violet and silver
staining) when evaluated 4 days later. In mice, LPS activated microglia (increased
lectin staining of morphologically identified cells) with substantially less
macrophage invasion but no neurodegeneration was seen at 4 days post LPS infusion.
To evaluate the role of infiltrating macrophages in the neurodegenerative response
in gerbils, peripheral macrophages were depleted by an intravenous injection of
liposome-encapsulated clodronate. This preparation depleted spleen and liver
macrophages (>95%), decreased blood monocytes by 55% and attenuated striatal
macrophage infiltration (32 to 73% in five representative sections). Notably, the
liposome-encapsulated clodronate reduced the severity of LPS-induced
neurodegeneration, as visualized by cresyl violet staining and quantified in 20
serially stained silver sections (total volume, 1.32+/-0.41 mm(3) in liposome-
encapsulated clodronate-treated versus 3.04+/-0.72 mm(3) in saline-treated
controls). These results indicate that a local LPS infusion in gerbil brain may be
a useful model in which to investigate the role of invading macrophages and other
inflammatory responses in neurodegeneration in inflammatory neurological disease.
AN - rayyan-553782240
AU - Zito, M. A.
AU - Koennecke, L. A.
AU - McAuliffe, M. J.
AU - McNally, B.
AU - van Rooijen, N.
AU - Heyes, M. P.
DO - 10.1016/s0006-8993(00)03135-8
IS - 1
J2 - Brain Res
KW - Analysis of Variance
Animals
Clodronic Acid/administration & dosage/*pharmacology
Corpus Striatum/*drug effects/pathology
Drug Carriers
Endotoxins/administration & dosage/toxicity
Gerbillinae
Infusions, Parenteral
Leukocytes/drug effects/physiology
Lipopolysaccharides/administration & dosage/*toxicity
Liposomes
Macrophages/*drug effects/pathology/physiology
Mice
Mice, Inbred C57BL
Nerve Degeneration/chemically induced/*prevention & control
Salmonella
Macrophages
LA - eng
N1 - Laboratory of Neurotoxicology, Building 10, Room 3D42, National Institute of
Mental Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
PY - 2001
SN - 0006-8993 (Print)
SP - 13-26
ST - Depletion of systemic macrophages by liposome-encapsulated clodronate
attenuates striatal macrophage invasion and neurodegeneration following local
endotoxin infusion in gerbils
T2 - Brain research
TI - Depletion of systemic macrophages by liposome-encapsulated clodronate
attenuates striatal macrophage invasion and neurodegeneration following local
endotoxin infusion in gerbils
VL - 892
Y2 - 2 y3 - 16
ID - 10150
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit
bacterial dental plaque because of their antimicrobial activity. Novel glutathione-
stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial
properties in food manufacturing processes. Therefore, this study aimed to evaluate
the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious
diseases. First, the antimicrobial activity of GSH-AgNPs against three oral
pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus
mutans) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in
inhibiting the growth of all bacteria, especially S. mutans (IC50 = 23.64 μg/mL, Ag
concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e., cell
viability) toward a human gingival fibroblast cell line (HGF-1), as an oral
epithelial model. Results indicated no toxic effects of GSH-AgNPs at low
concentrations (≥6.16 μg/mL, Ag concentration). Higher concentrations resulted in
losing cell viability, which followed the Ag accumulation in cells. Finally, the
inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was
measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8)
and tumor necrosis factor-alpha (TNF-α)). GSH-AgNPs activates the inflammatory
response in human gingival fibroblasts, increasing the production of cytokines.
These findings provide new insights for the use of GSH-AgNPs in dental care and
encourage further studies for their application. © 2020 by the authors.
AN - rayyan-553781751
AU - Zorraquín-Peña, I.
AU - Cueva, C.
AU - de Llano, D. G.
AU - Bartolomé, B.
AU - Moreno-Arribas, M. V.
DO - 10.3390/BIOMEDICINES8100375
IS - 10
KW - Antimicrobial activity
Cytotoxicity
cytokines
Oral bacteria
Periodontal pathogens
Glutathione
Periodontics
PY - 2020
ST - Glutathione-stabilized silver nanoparticles: Antibacterial activity against
periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
T2 - Biomedicines
TI - Glutathione-stabilized silver nanoparticles: Antibacterial activity against
periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
85092586023&doi=10.3390%2fBIOMEDICINES8100375&partnerID=40&md5=66a01c350a759453eef9
b192ed0bf22c
VL - 8
ID - 9684
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit
bacterial dental plaque because of their antimicrobial activity. Novel glutathione-
stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial
properties in food manufacturing processes. Therefore, this study aimed to evaluate
the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious
diseases. First, the antimicrobial activity of GSH-AgNPs against three oral
pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus
mutans) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in
inhibiting the growth of all bacteria, especially S. mutans (IC50 = 23.64 mu g/mL,
Ag concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e.,
cell viability) toward a human gingival fibroblast cell line (HGF-1), as an oral
epithelial model. Results indicated no toxic effects of GSH-AgNPs at low
concentrations (<= 6.16 mu g/mL, Ag concentration). Higher concentrations resulted
in losing cell viability, which followed the Ag accumulation in cells. Finally, the
inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was
measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8)
and tumor necrosis factor-alpha (TNF-alpha)). GSH-AgNPs activates the inflammatory
response in human gingival fibroblasts, increasing the production of cytokines.
These findings provide new insights for the use of GSH-AgNPs in dental care and
encourage further studies for their application.
AN - rayyan-553781752
AU - Zorraquin-Pena, I.
AU - Cueva, C.
AU - de Llano, D. G.
AU - Bartolome, B.
AU - Moreno-Arribas, M. V.
IS - 10
KW - Glutathione
Periodontics
PY - 2020
SN - 2227-9059
ST - Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against
Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells
T2 - BIOMEDICINES
TI - Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against
Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells
VL - 8
Y2 - 10
ID - 9685
ER -
TY - JOUR
AB - Liposome nanocarriers (LPNs) are potentially the future of inner ear therapy
due to their high drug loading capacity and efficient uptake in the inner ear after
a minimally invasive intratympanic administration. However, information on the
biocompatibility of LPNs in the inner ear is lacking. The aim of the present study
is to document the biocompatibility of LPNs in the inner ear after intratympanic
delivery. LPNs with or without gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid
(Gd-DOTA) were delivered to the rats through transtympanic injection. The
distribution of the Gd-DOTA-containing LPNs in the middle and inner ear was tracked
in vivo using MRI. The function of the middle and inner ear barriers was evaluated
using gadolinium-enhanced MRI. The auditory function was measured using auditory
brainstem response (ABR). The potential inflammatory response was investigated by
analyzing glycosaminoglycan and hyaluronic acid secretion and CD44 and TLR2
expression in the inner ear. The potential apoptosis was analyzed using terminal
transferase (TdT) to label the free 3'OH breaks in the DNA strands of apoptotic
cells with TMR-dUTP (TUNEL staining). As a result, LPNs entered the inner ear
efficiently after transtympanic injection. The transtympanic injection of LPNs with
or without Gd-DOTA neither disrupted the function of the middle and inner ear
barriers nor caused hearing impairment in rats. The critical inflammatory
biological markers in the inner ear, including glycosaminoglycan and hyaluronic
acid secretion and CD44 and TLR2 expression, were not influenced by the
administration of LPNs. There was no significant cell death associated with the
administration of LPNs. The transtympanic injection of LPNs is safe for the inner
ear, and LPNs may be applied as a drug delivery matrix in the clinical therapy of
sensorineural hearing loss.
AN - rayyan-553781753
AU - Zou, J.
AU - Feng, H.
AU - Sood, R.
AU - Kinnunen, P. K. J.
AU - Pyykko, I.
DO - 10.1186/s11671-017-2142-5
KW - Rats
Liposomes
Ear, Inner
Ear
PY - 2017
SN - 1556-276X
ST - Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After
Intratympanic Administration
T2 - NANOSCALE RESEARCH LETTERS
TI - Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After
Intratympanic Administration
VL - 12
Y2 - 5 y3 - 25
ID - 9686
ER -
TY - JOUR
AB - Asthma is a chronic airway inflammatory disease with complex mechanisms, and
these patients often encounter difficulties in their treatment course due to the
heterogeneity of the disease. Currently, clinical treatments for asthma are mainly
based on glucocorticoid-based combination drug therapy; however, glucocorticoid
resistance and multiple side effects, as well as the occurrence of poor drug
delivery, require the development of more promising treatments. Nanotechnology is
an emerging technology that has been extensively researched in the medical field.
Several studies have shown that drug delivery systems could significantly improve
the targeting, reduce toxicity and improve the bioavailability of drugs. The use of
multiple nanoparticle delivery strategies could improve the therapeutic efficacy of
drugs compared to traditional delivery methods. Herein, the authors presented the
mechanisms of asthma development and current therapeutic methods. Furthermore, the
design and synthesis of different types of nanomaterials and micromaterials for
asthma therapy are reviewed, including polymetric nanomaterials, solid lipid
nanomaterials, cell membranes-based nanomaterials, and metal nanomaterials.
Finally, the challenges and future perspectives of these nanomaterials are
discussed to provide guidance for further research directions and hopefully promote
the clinical application of nanotherapeutics in asthma treatment.
AN - rayyan-553781754
AU - Zuo, X.
AU - Guo, X. P.
AU - Gu, Y. N.
AU - Zheng, H. Y.
AU - Zhou, Z. J.
AU - Wang, X. L.
AU - Jiang, S. Y.
AU - Wang, G. Q.
AU - Xu, C. N.
AU - Wang, F.
DO - 10.3390/ijms232214427
IS - 22
KW - Asthma
PY - 2022
SN - 1422-0067
ST - Recent Advances in Nanomaterials for Asthma Treatment
TI - Recent Advances in Nanomaterials for Asthma Treatment
VL - 23
Y2 - 11
ID - 9687
ER -

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AB - The proceedings contain 2 papers. The topics discussed include: carbonaceous

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