Tris-HCl pH 7.

semnificatie m proba (gV extr (mL)

r CTRL 1 0.2093 3
r AMX 4 mg/L 2 0.2031 3
r CIP 4 mg/L 3 0.2056 3
r AMX_CIP(4+4) 4 0.202 3
f CTRL 5 0.2037 3
f AMX 4 mg/L 6 0.2041 3
f CIP 4 mg/L 7 0.2048 3
f AMX_CIP(4+4) 8 0.201 3
 20" 30"
A436
m proba (gV extr (mL) dA/dt Vproba (mL) Vtotal (mL) e AE (U/mL) g sp/mL AS(U/g sp)
1 0.2093 3 0.8453 0.1 3.3 25.5 4.38 0.07 62.72
2 0.2031 3 0.6716 0.1 3.3 25.5 3.48 0.07 51.35
3 0.2056 3 1.12 0.1 3.3 25.5 5.80 0.07 84.60
4 0.202 3 1.18 0.1 3.3 25.5 6.11 0.07 90.72
5 0.2037 3 0.236 0.2 3.4 25.5 0.63 0.07 9.27
6 0.2041 3 0.276 0.2 3.4 25.5 0.74 0.07 10.82
7 0.2048 3 0.3648 0.2 3.4 25.5 0.97 0.07 14.25
8 0.201 3 0.3128 0.2 3.4 25.5 0.83 0.07 12.45

Peroxidaza
POX radaci10*POX frunza Activitatea peroxidazelor (U/g s.p.
CTRL 62.72 92.69
AMX 4 mg/L 51.35 108.18 200.00 POX ra
CIP 4 mg/L 84.60 142.50 10*PO
AMX_CIP(4+4 90.72 124.50 150.00
POX (U/g s.p.)

100.00

50.00

0.00
CTRL AMX 4 mg/L CIP 4 mg/L AMX_CIP(4+4
)
Varianta experimentala
 cprot
rad/frz % control (mg/mL)
6.8 100.00
4.7 81.87
5.9 134.88
7.3 144.64
99.98
116.70
153.72
134.30

xidazelor (U/g s.p.)

POX radacina
10*POX frunza

CIP 4 mg/L AMX_CIP(4+4

)
mentala
probe frunze

m proba (gV extr (mL)A500 Vproba S (μmol/mL)

c glucoza (μmol/mL) g sp/mL c glucoza (μmol/g sp)
1 0.2093 3 0.2768 0.05 5 1.15 0.0698 16.41
2 0.2031 3 0.2568 0.05 5 1.01 0.0677 14.93
3 0.2056 3 0.3483 0.05 5 1.63 0.0685 23.73
4 0.202 3 0.3069 0.05 5 1.35 0.0673 20.02
5 0.2037 3 0.724 0.05 5 4.16 0.0679 61.20
6 0.2041 3 0.6 0.05 5 3.32 0.0680 48.81
7 0.2048 3 1.7242 0.05 5 10.89 0.0683 159.51
8 0.201 3 1.1754 0.05 5 7.19 0.0670 107.38
blanc (B) 0.1067
standard (S) 0.9087
Cantitatea de glucoza (μmol/g s.p.)
glucoza (μmol/g s.p.) 180.00
radacina frunza 160.00
CTRL 16.41 61.20 140.00

Glucza (μol/g s.p.)

AMX 4 mg/L 14.93 48.81 120.00
23.73 159.51 100.00
CIP 4 mg/L
80.00
AMX_CIP(4+4 20.02 107.38
60.00
40.00
20.00
0.00
CTRL AMX 4 mg/L CIP 4 mg/L AMX_CIP(4+4
Varianta experimentală
 c glucoza (μmol/g sp) frz/rad % control
100.02
90.96
144.62
121.98
3.73 100.34
3.27 80.02
6.72 261.49
5.36 176.04

coza (μmol/g s.p.)

radacina
frunza

/L CIP 4 mg/L AMX_CIP(4+4)

erimentală
probe frunze

m proba (gV extr (mL) A595 Vproba (mL) Vreactiv (mL)

c proteine (μg/mL)
g sp/mL
c proteine c(μg/g
proteine
s.p.) (mg/g s.p.)
1 0.2093 3 0.3927 0.2 5 287.7143 0.07 4123.951 4.12
2 0.2031 3 0.4297 0.2 5 340.5714 0.07 5030.597 5.03
3 0.2056 3 0.5316 0.2 5 486.1429 0.07 7093.524 7.09
4 0.202 3 0.5055 0.2 5 448.8571 0.07 6666.195 6.67
5 0.2037 3 0.588 0.2 5 566.7143 0.07 8346.308 8.35
6 0.2041 3 0.5915 0.2 5 571.7143 0.07 8403.444 8.40
7 0.2048 3 0.653 0.2 5 659.5714 0.07 9661.691 9.66
8 0.201 3 0.6081 0.2 5 595.4286 0.07 8886.994 8.89

c proteine Concentrația proteinelor

mg/g s.p.
12.00
radacina frunza
CTRL 4.12 8.35 10.00

c proteine (mg/ g s.p.)

AMX 4 mg/L 5.03 8.40
8.00
CIP 4 mg/L 7.09 9.66
AMX_CIP(4+4 6.67 8.89 6.00

4.00

2.00

0.00
CTRL AMX 4 mg/L CIP 4 mg/L A
Varianta experimentală
 curba etalon Bradford
frz/rad % control μg prot/mL Curba etalon proteine_Bradford
100.10 A595
0.35
122.10 50 0.2225
172.17 100 0.26 f(x) = 0.000687 x + 0.19125
0.3 R² = 0.993928634606151
161.80 200 0.33
2.02 99.96 0.25
A

A595
1.67 100.64 Li
0.2
1.36 115.71
1.33 106.43 0.15

0.1
radacina
trația proteinelor frunza
40 60 80 100 120 140 160 180 200 220
μg prot/mL

MX 4 mg/L CIP 4 mg/L AMX_CIP(4+4)

rianta experimentală
roteine_Bradford

9125
51

A595
Linear (A595)

0 160 180 200 220

L
probe frunze TBARS
(nmol/mL) (nmol/g sp)
m proba (gV extr (mL) A535 Vproba (mL) Vtotal (mL) e A535 corectat
Prod sec pe g sp/mL
1 0.2093 3 0.1852 0.5 3 0.149 0.1211 4.88 0.07
2 0.2031 3 0.2745 0.5 3 0.149 0.2104 8.47 0.07
3 0.2056 3 0.2451 0.5 3 0.149 0.181 7.29 0.07
4 0.202 3 0.28 0.5 3 0.149 0.2159 8.69 0.07
5 0.2037 3 0.2251 0.5 3 0.149 0.161 6.48 0.07
6 0.2041 3 0.2306 0.5 3 0.149 0.1665 6.70 0.07
7 0.2048 3 0.2995 0.5 3 0.149 0.2354 9.48 0.07
8 0.201 3 0.2916 0.5 3 0.149 0.2275 9.16 0.07
blanc 0.0641

TBARS Produși secundari de peroxidare

(nmol/g sp) a lipidelor (TBARS)
radacina frunza
160.00
CTRL 69.90 95.48
AMX 4 mg/L 125.15 98.55 140.00
CTBARS (nmol/g s.p.) 120.00
CIP 4 mg/L 106.35 138.86
AMX_CIP(4+4 129.12 136.73 100.00
80.00
60.00
40.00
20.00
0.00
CTRL AMX 4 mg/L CIP 4 mg/L AMX_CIP(4+4
Varianta experimentală
 TBARS
(nmol/g sp)
Prod sec perox lipide
frz/rad % control
69.90 100.00
125.15 179.04
106.35 152.15
129.12 184.72
95.482 1.37 100.00
98.55 0.79 103.22
138.86 1.31 145.43
136.73 1.06 143.21

ari de peroxidare
or (TBARS)

radacina
frunza

/L CIP 4 mg/L AMX_CIP(4+4)

erimentală
3 mL etanol varf frz baza frz ctrl v ctrl b
(g) (g) Lungimea de undaAbs Abs
CTRL 1 0.0294 0.0291 664 0.6909 0.4021
AMX 4 mg/ 2 0.0332 0.0342 648 0.3495 0.2292
CIP 4 mg/L 3 0.032 0.0327 470 0.5649 0.3239
AMX_CIP(4 4 0.0308 0.0321
μmol/mL
mproba (g) Vetanol A648 A664 A470 Cl b Cl a Carotenoizi
v CTRL 0.0294 3 0.3495 0.6909 0.5649 0.00478 0.008635 0.002813
v AMX 4 m 0.0332 3 0.3495 0.7106 0.5699 0.004691 0.008884 0.002832
v CIP 4 mg 0.032 3 0.4779 1.0321 0.8789 0.006145 0.01291 0.004637
v AMX_CIP 0.0308 3 0.3995 0.8489 0.7179 0.005198 0.010616 0.003752
b CTRL 0.0291 3 0.2292 0.4021 0.3239 0.003363 0.00502 0.001514
b AMX 4 m 0.0342 3 0.2608 0.7106 0.4085 0.002692 0.008903 0.001763
b CIP 4 mg 0.0327 3 0.2739 0.5066 0.4521 0.003903 0.006328 0.002324
b AMX_CIP 0.0321 3 0.3813 0.7870 0.6521 0.005066 0.00984 0.003331
 amx v amx b cip v cip b amx+cip v amx+cip b
Abs Abs Abs Abs Abs Abs
0.7106 0.5150 1.0321 0.5066 0.8489 0.7870
0.3495 0.2608 0.4779 0.2739 0.3995 0.3813
0.5699 0.4085 0.8789 0.4521 0.7179 0.6521

μmol/g sp
Carotenoizi g sp/mL Cl b Cl a Carotenoizi
0.0098 0.487782 0.881098 0.287046
0.011067 0.423876 0.802762 0.255931
0.010667 0.5761 1.210297 0.434728
0.010267 0.506286 1.03407 0.365493
0.0097 0.34674 0.517563 0.156045
0.0114 0.236116 0.781001 0.154643
0.0109 0.358082 0.58056 0.213218
0.0107 0.47342 0.919656 0.311286
pig
Chl b (μmol/g s.p.) Chl a μmol/g s.p)
v b v b
CTRL 0.487782 0.34674 CTRL 0.881098 0.517563
AMX 4 mg/ 0.423876 0.236116 AMX 4 mg/ 0.802762 0.781001
CIP 4 mg/L 0.5761 0.358082 CIP 4 mg/L 1.210297 0.58056
AMX_CIP(4 0.506286 0.47342 AMX_CIP(4 1.03407 0.919656

Clorofila b Clorofi
0.7 1.4
0.6 1.2

Clorofila a (μmol/g s.p.)

Clorofila b (μmol/g s.p.)

0.5 1
0.4 v 0.8
0.3 b 0.6
0.2 0.4
0.1 0.2
0 0
CTRL AMX 4 mg/L CIP 4 mg/L AMX_CIP(4+4) CTRL AMX 4 mg/L
Varianta experimentală Varianta expe
 Car(μmol/g s.p)
v b
CTRL 0.287046 0.156045
AMX 4 mg/ 0.255931 0.154643
CIP 4 mg/L 0.434728 0.213218
AMX_CIP(4 0.365493 0.311286

Clorofila a Carotenoizi
0.5
0.45

Carotenoizi (μmol/g s.p.)

0.4
0.35
0.3
v
0.25
b
0.2
0.15
0.1
0.05
0
CTRL AMX 4 mg/L CIP 4 mg/L AMX_CIP(4+4) CTRL AMX 4 mg/L CIP 4 mg/L AMX_CIP(4+
Varianta experimentală Varianta experimentală
enoizi

v
b

L CIP 4 mg/L AMX_CIP(4+4)

rianta experimentală