EXPERIMENT 7

Test Purpose Materials Used Positive Result Negative Result Picture

Test for Reagents Formation of one layer Formation of two layers

Solubility in the sol’n in the sol’n
- water
- diluted HCl
- diluted NaOH - coconut oil + water (H2O)
- hot, cold alcohol - coconut oil + dil.
- determines how much - coconut oil + ether (C4H10O)
Solubility Test - ether hydrochloric acid (HCl)
solute is or can be - coconut oil + chloroform
(for coconut oil) - CHCl3 - coconut oil + dil. sodium
dissolved in a solvent (CHCl3)
- CCl4 hydroxide (NaOH)
at equilibrium - coconut oil + carbon
- coconut oil + cold alcohol
- nonpolar attracts tetrachloride (CCl4)
Reactants (C2H5OH)
nonpolar, polar attracts - glycerol + water (H2O)
- coconut oil + hot alcohol
polar - glycerol + alcohol (C2H5OH)
- coconut oil (nonpolar) (C2H5OH)
- glycerol (polar) - glycerol + ether (C4H10O)

Test for Reagents Blue-Black coloration No change in coloration

pH of the sol’n with Congo Red of the sol’n with Congo Red

- congo red
(C32H22N6Na2O6S) - Coconut oil
- litmus paper - Rancid oil
(red)

- fresh oil is neutral but Reactants

Reaction towards Blue coloration No change in coloration
when exposed to an
Indicators of the Red Litmus Paper of the Red Litmus Paper
open environment, it
undergoes oxidation
called rancidity - Coconut oil
- (-OH) in oils will turn - Rancid oil
to (COOH) - fresh coconut oil
- rancid oi Red coloration No change in coloration
of the Blue Litmus Paper of the Blue Litmus Paper

- rancid oil - coconut oil

Test for Presence of Material Translucent Spot Formation No Translucent Spot

Translucent Spot in the paper Formation in the paper
- piece of paper

Reactants
Formation of - essential oils
Translucent Spot (coconut oil) wouldn’t
leave translucent spots - coconut oil
unless they’re actual
oils - coconut oil

Test for Reagents Piercing Acrid/Burnt

Acrolein Formation Fat/Burnt Yeast odor

- process: dehydration - KHSO4

- acrolein is the
Acrolein Test
simplest unsaturated
- coconut oil
aldehyde caused by
Reactants - glycerol (C3H8O3)
dehydration of glycerol
- KHSO4 as
dehydrating agent - coconut oil
- glycerol

Test for Reagents White ppt No ppt

Miscibility in clear sol’n in clear sol’n
- water
- water and soap sol’n
- water and albumin - coconut oil + water and soap
- water and sodium sol’n (permanent emulsion) add another picture for reference
Emulsification - combining 2 liquids carbonate - coconut oil + water and kay wala nakoy lain sources :((
that do not usually mix albumin (temporary emulsion)
- coconut oil + water (H2O)
together with the use of Reactants - coconut oil + water and
an emulsifying agent sodium carbonate
(H2O + Na2CO3)
- coconut oil (temporary emulsion)

Test for Reagents Brown coloration No coloration

Double Bonds (unsaturated) in clear sol’n (saturated) in clear sol’n
- Hubl’s I2 Solution
a.) HgCl2 in 95%
C2H5OH
b.) I2 in 95%
Test for C2H5OH
- fats that contain - stearic acid (lightest)
Unsaturation
unsaturated fatty acids - oleic acid
Reactants
will have a positive - coconut oil
result - linseed oil (darkest)
- oleic acid
- stearic acid
- coconut oil
- linseed oil

Test for the presence Reagents Green flame on the tip Red-Orange flame on the tip
of Glycerol Esters of the Nichrome Wire of the Nichrome Wire
- Powdered Borax
a.) Na2B4O7(H2O)10
Flame Test
(for glycerol) - A green flame is Reactants
produced if glycerol is - glycerol (C3H8O3)
fused with boric acid
- glycerol

Test for Reagents Blue coloration

1o and 2o Alcohols of the sol’n
- conc. HNO3
- K2CrO4
Nitro-Chromic - detection of primary Reactants
Acid Test and secondary alcohol
- the hydroxyl group - glycerol (C3H8O3)
(-OH) of glycerol reacts
with the reagents - glycerol