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  • Chemistry Experiment No. 13

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    kokatedhananjay68
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    The document provides instructions for detecting the presence of proteins in a sample using common chemical tests. It lists the apparatus, chemicals, and theory needed, and shows an observations table for the Biuret test and Xanthoproteic test, which produced results indicating the sample contains protein.

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    Practil very important

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    The document provides instructions for detecting the presence of proteins in a sample using common chemical tests. It lists the apparatus, chemicals, and theory needed, and shows an observations table for the Biuret test and Xanthoproteic test, which produced results indicating the sample contains protein.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    7 views1 page

    Chemistry Experiment No. 13

    Uploaded by

    kokatedhananjay68
    The document provides instructions for detecting the presence of proteins in a sample using common chemical tests. It lists the apparatus, chemicals, and theory needed, and shows an observations table for the Biuret test and Xanthoproteic test, which produced results indicating the sample contains protein.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    ObjectThe detect the presence of proteins in a given sample.

    Apparatus Required Test tubes, test tube stand, filter paper, glass rod, beaker 5.
    etc.
    Chemicals required Coppersulphate solution, NaOH solution Ninhydrin. Ptz
    sulphate, Nitrie acid and egg albumin dispersion, gelatin dispersion etc.
    Theory-Proteins are large group of organic compounds found in all living orgzism
    They comprise hydrogen, oxygen, nitrogen, sulphur and phosphorous in different propur
    Observations Table
    S.No. Experimnent Observations Inference
    1. Biuret Test : Take 5% egg Blue-violet colouration Proteins presenL
    albumin and add 2 ml NaOH +appears
    4-5 drops 1% CuSO4 solution
    2. Xanthoproteic Test : Yellow ppt. is obtained. Protein present
    Take 2 ml óf egg albumin
    dispersion + few drops conc.
    HNO3 and heat
    ResultThe given sample contains protein.

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