Corning® Synthemax® II-SC Substrate

synthetic self-coating substrate for pluripotent stem cell culture

Pluripotency marker expression in hiPSCs during 5 passages

 Animal-free, synthetic on Synthemax II-SC Substrate
alternative for feeder layers
and biological coatings

Positive cells (%)

 Easy to coat
Matrigel™
 Lot traceable Synthemax Surface
Synthemax II-SC Substrate
 Gamma irradiated
 Quality tested for lot-to-lot
consistency Flow cytometery data
Average of 5 passages +/- STDEV
Cat. # 3535XX1 OCT4 SSEA4

hiPSC doubling time during 7 serial passages on hiPSCs cultured on Synthemax II-SC Substrate for 7
Synthemax II-SC Substrate passages maintain the ability to differentiate to cells of
all three germ layers
Doubling time (hours)

β-III-Tubulin (Ectoderm) α-Actinin (Mesoderm) AFP (Endoderm)

hiPSCs maintain normal karyotype after multiple

Passage number passages on Synthemax II-SC Substrate

OCT4 immunofluorescent staining of hiPSCs after 5

passages on Synthemax II-SC Substrate

Synthemax II-SC Substrate Matrigel™

OCT4

Summary
 Synthemax II-SC Substrate coated plates
Hoechst

support multipassage culture of hiPSCs in

defined medium
 hiPSCs cultured on Synthemax II-SC Substrate
coated plates maintain pluripotency markers,
normal karyotype and ability to differentiate
Corning and Synthemax are the registered trademarks of Corning to cells of all three germ layers
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