Lab Report:9

Titratable Acidity of Fruit Juice

Dhwani Mistry

C0866952

FSQ 3304 2

Prof. Masomeh Ghassem

Contents
Objective....................................................................................................................................... 3
Materials........................................................................................................................................ 3
Procedure:...................................................................................................................................... 3
Calculation:....................................................................................................................................4
Observation images:...................................................................................................................... 5
Conclusion:.................................................................................................................................... 5
Laboratory Manual: Measurement of Titratable Acidity in Wine

Objective: The objective of this experiment is to determine the titratable acidity of

fruit juice by titrating it with a standard sodium hydroxide (NaOH) solution.

The titration is based on the reaction between citric acid (C6H8O7) in the fruit
juice and NaOH, where three moles of NaOH are required to neutralize one mole
of citric acid. The molecular weight of citric acid is 192.124 g/mol.

Materials:
 20 mL sample of wine, milk or fruit juice(unknown concentration)
 30 mL deionized water
 Phenolphthalein indicator solution
 0.1 M NaOH titrant
 Burette
 Erlenmeyer flask (250 mL)
 Pipette (25 mL)
 Beaker (100 mL)
 Lab coat and safety glasses

Procedure:
 Firstly, rinse the pipette with the fruit juice sample, and then measure 10 ml
sample(fruit juice) into the Erlenmeyer flask.
 Measure 20 ml deionized water to the flask with fruit juice.
 Add few drops of Phenolphthalein indicator to this sample water mixture,
this will assist identifying the end-point when the color changes to pink.
 Prepare 0.1M NaOH solution and fill the burette. Record the initial volume
of the burette.
 Now, titrate the sample-water mixture with 0.1M NaOH, by continuously
swirling the flask thoroughly.
 As the end-point approaches, and there is visible faint pink color which
fades while swirling,
 Continue the titration until a permanent pink color is attained by the sample-
mixture, which is the end point.
  Record the final volume from the burette, to note how much NaOH is
consumed.
 Calculate the titratable acidity.

Calculation:

 Molecular weight of citric acid=192.124 g/mol

 The chemical reaction of the titration is as follows:
C6H8O7+3NaOH→Na3C6H5O7+3H2O (Citric Acid)

STEP 1: Measure the volume of NaOH

The titration was performed 3 times to achieve accurate results. Following
are the amounts of NaOH utilized in each titration:
o V1= 12.2ml
o V2= 13.0 ml
o V3= 14.0ml
 Average volume of NaOH(ml) utilised = V1+V2+V3/3
= 12.2+ 13.0+14.0/3
=13.066 ml
= 0.01306 L

STEP 2: Calculate number of moles moles of NaOH used (nNaOH) using the
formula n=C×V where:
o C is the concentration of NaOH ,
o V is the volume in liters
N = C*V
= 0.1* 0.01306
= 0.001306 moles of NaOH
STEP 3: Since the reaction between citric acid (C6H8O7) and NaOH is a 1:3 ratio,
the moles of citiric acid (n C6H8O7) is one-third the moles of NaOH:
Number of moles citric acid = 1/3rd of n(NaOH)
=0.001306/3
= 0.00043533333 moles

STEP 4: The amount of fruit juice used is 20 mL, which is 0.02 L. The
concentration (CC6H8O7) of citric acid in the fruit juice sample is then:

o nC6H8O7 =0.0004/0.02= 0.02 mol/L

STEP 5: To convert this concentration to grams per liter, multiply by the molar
mass of citric acid (192.124 g/mol)
o 0.02 mol/l * 192.124 g/mol
o 3.842 g/mol

The titratable acidity of the fruit-juice is expressed as 3.842 g/L of citric acid.

Observation images:

Figure 1 Fruit-Juice Sample

Figure 2 Color change comparison of Titrated juice and
untitrated juice

Figure 3 Image of 3 Titrated sample and original fruit juice sample.

Conclusion:
In this experiment, titration was performed to see the titration acidity of fruit-juice.
To determine the normality of sodium hydroxide standardization of NaOH was
done. The calculated normality was 0.001306 moles. After that, TA for citric acid
was calculated which was 3.842 g/mol.