Maintenance Guideline for Maglumi Series

--Overseas Technical Department

版本号：20161024
CONTENTS

01
PART ONE Monthly Maintenance 02
PART TWO Needle position

03
PART THREE Washer lift position 04
PART FOUR Chamber cleaning

05
PART FIVE Starter injector 06
PART SIX
pump volume

07
PART SEVEN
<Background> 08
PART EIGHT
<Plateau>

FOCUS MAKES PROFESSION P2

01
PART ONE
Monthly Maintenance
01
PART ONE Monthly Maintenance
Conduct monthly maintenance as per the guidance in User software

FOCUS MAKES PROFESSION P4

02
PART TWO
Needle position
02
PART TWO Needle position
First sampling and second sampling after incubation (for Maglumi 600&800);
left pipetting position and incubator pipetting position (for Maglumi 1000 and above):

Fig. left pipetting position and incubator pipetting

Fig. First sampling and second sampling after
position (for Maglumi 1000 and above)
incubation（for Maglumi 600 & Maglumi 800）

FOCUS MAKES PROFESSION P6

02
PART TWO Needle position
Adjust the corresponding positions as showed below:

Note: When adjusting the needle position, it is required to confirm the position from the front and
the left or right side of the cuvette.

FOCUS MAKES PROFESSION P7

02
PART TWO Needle position
Second sampling after wash (for Maglumi 600&800);
Right pipetting position (For M1000 and above)

Fig. Second sampling after wash Fig right pipetting position (for
Maglumi 1000 and above)

FOCUS MAKES PROFESSION P8

 02
PART TWO Needle position
Adjust the corresponding positions as showed below:

Fig Position of 1 and 6 Fig Position of Mix

Note:
When adjusting the needle position, it is required to confirm the position from the front and the left or right
side of the cuvette.
When adjusting the position of right pipetting area or second sampling after wash, it is required to move the
reaction module from the pusher to backtransport.
FOCUS MAKES PROFESSION P9
03
PART THREE
Needle position

FOCUS MAKES PROFESSION P10

03
PART Three Washer lift position

Fig Washer lift positions adjustment

FOCUS MAKES PROFESSION P11
03
PART Three Washer lift position
Content Steps Note
Select “Inject” in the “Washer
Adjustment Steps ： Step 1 Lift” dialog.
Choose “Stepsize”.

move to Click to adjust the inject

“Inject” position of the washer lift.
position Make sure the inject needles are
Step 2
at the opening of the cuvette.
Click <Writeparam> to save
the parameters.

Select “Suction” and choose

Step 1
“Stepsize”

Click to adjust the suction

position of the washer lift.
“Sution” Make sure the suction needles
just touch the cuvette’s bottom
Step 2
and the spring flakes of the
needles bounce up by 1mm.
Click <Writeparam> to save
the parameters

FOCUS MAKES PROFESSION P12

03
PART Three Washer lift position

Fig “Inject” position: all the inject needles shall be at the opening of the cuvette

FOCUS MAKES PROFESSION P13

04
PART FOUR
Chamber cleaning

FOCUS MAKES PROFESSION P14

04
PART Four
Chamber cleaning

•Before removal of
chamber cover, check the
“Background” in service
software making sure that
there is no light leakage.

FOCUS MAKES PROFESSION P15

04
PART Four
Chamber cleaning
Remove the chamber cover.
Note: It is necessary to remove the flat cable connected to PMT in case of influence on PMT after the chamber
cover is taken out

FOCUS MAKES PROFESSION P16

04
PART Four
Chamber cleaning

FOCUS MAKES PROFESSION P17

05
PART FIVE
Starter injector & lift

FOCUS MAKES PROFESSION P18

05
PART Five
Starter injector and Chamber lift position

Fig Make sure the starter tubes are 1-2 mm out of the starter
injector and stable during the movement of chamber lift. In
case of adjustment needed, adjust the screws marked 1 or/and 2

FOCUS MAKES PROFESSION P19

 05
PART Five
Starter injector and Chamber lift position

Check cuvette position in

chamber:
1. Chamber Transport
Insert a reaction module
to the pusher and move
the pusher to “Chamber”
position.
2. Click “Cham Trans”
button and move the
reaction module 8 steps
inside the chamber
transport with “Move Fig Insert a reaction module to pusher and move the pusher to
Count (Recoup) “Chamber” position

FOCUS MAKES PROFESSION P20

 05
PART Five
Starter injector and Chamber lift position

3. Make sure that the starter injector is

right above the cuvette. If not, initialize
the chamber lift, turn to “Cham Trans”,
increase or decrease the value of
“Adjust pos” and “Writeparam”, click
“Initialize” and repeat Step a until the
starter injector is right above the
cuvette.

Note: “Adjust pos” could be changed by

10-20 each time.

Fig Chamber Transport interface

FOCUS MAKES PROFESSION P21
05
PART Five
Starter injector and Chamber lift position

4. After confirming that the

starter injector is right above
the cuvette, check the “Inject”
position and make sure the
starter tubes are right at the
opening of the cuvette.

Note: as the starter tubes are 1-2

mm out of the starter injector,
the proper position of “Inject”
requires the starter injector be
1-2 mm higher than the cuvette.

Fig Inject position

FOCUS MAKES PROFESSION P22
06
PART SIX
Pump volume

FOCUS MAKES PROFESSION P23

06
PART SIX
Pump volume
Prime the chamber and washer in service software and make sure that the tubes of washer and
chamber are fully filled with system liquid or starters.

Fig Priming for Washer and Chamber

FOCUS MAKES PROFESSION P24
 06
PART SIX
Pump volume
Turn off the instrument and pull up the Washer lift and turn on the instrument again without initialization.

Fig Lift up the washer lift

FOCUS MAKES PROFESSION P25
06
PART SIX
Pump volume

Check the pump volume of washer pumps and starter pumps.

Note: The pump volume for

washer pumps shall be 400 ul
and shall align to the 400ul
liquid line of the cuvette. The
pump volume for starter
pumps shall be 200 ul and 2
times of injection shall align to
the 400ul liquid line of the
cuvette. In case of anomalous
pump volume, adjust the
volume of the corresponding
pump(s).

Fig Volume checking

FOCUS MAKES PROFESSION P26
07
PART SEVEN
Background

FOCUS MAKES PROFESSION P27

07
PART Seven
Background

Click “Globals” and “Background” to

check the background

Fig Background
FOCUS MAKES PROFESSION P28
07
PART Seven

Content
Background
Steps Note
Step 1 Click <Initialization> in the main menu to
initialize the analyzer.
Step 2 Click the <Globals> — <background> to
open the “background” dialog.

Step 3 Input the “Meas.time” and “Meas.cycles”

(Use default value commonly).
Choose the check box among the four kinds 1. Please ensure
of measurement. that the
Then press <Start.meas>. “initialization” has
Dark Count Step 4 1.”Background/Closed” <80 been done in the
measurement ”Background/Open” <80 first place
under different ”Background/Trans” <80 2. Do not open the
models If either of the above is out of range, light cover of the
leakage is suspected . Refer to SNIBE After- chamber or
sales specialist. disconnect the
2. The value of the “Ref. LED on” is cable of the PMT
supposed to be between 140,000-230,000,
This value can be adjusted by changing the
value of the “D/A Ref Led” on the “Globals”
interface. If it’s bigger, the value of “Ref.
LED on” in “Background” will be higher.
FOCUS MAKES PROFESSION P29
08
PART EIGHT
Plateau

FOCUS MAKES PROFESSION P30

08
PART Eight
Plateau
Plateau is to decide the high voltage for PMT and to check the performance of PMT. It
works combining with the Reference LED; therefore, make sure the value of “Ref. LED
on” in “Background” is between 140,000 and 230,000 before testing <Plateau>.

Fig Plateau
FOCUS MAKES PROFESSION P31
 08
PART Eight
Plateau
Content Steps Note
Step 1 Click <Globals>—<Plateau> to open the
“Plateau” dialog.
Step 2 Input “300000” in “Max RLU”, choose
“50000” in “Scal.”, and click the <Draw
1. Please ensure
diagr.> button. Tick “Reference LED on”.
that the
Step 3 Click the <Start> button to draw the D/A
“initialization” has
value curve. It should be smooth as shown in
been done in the
the figure below. If not, contact SNIBE after-
first place
sales specialist.
PMT 2. Choose the
Step 4 After getting the smooth curve, note down the
Voltage’s right value of the
“Suggest D/A highvolt”. Click <Start>
setting “D/A Ref Led” to
button for another 4 times to record the
make sure the
“Suggest D/A highvolt”. The difference
value of the “Ref.
between these 5 values shall be no more than
LED on” is
5. If not, adjust the value of the “D/A Ref
between 140,000
Led” and repeat Step 2 to Step 4.
and 230,000
Step 5 When we get all the values of “Suggest D/A
highvolt”. Input the smallest one in “D/A
highvolt” in the “Global” interface and click
“Writeparam”.
FOCUS MAKES PROFESSION P32
08
PART Eight
Plateau

Fig The curve shall be smooth and among the five times of tests,
“Suggest D/A Highvolt” shall not exceed 170.
FOCUS MAKES PROFESSION P33
 Thanks
FOCUS MAKES PROFESSION