Class : DRT-1 Marks : 25

Date : 22.11.2023 BIOLOGY Duration : 1 hour

I. ANSWER ALL THE QUESTIONS : (5x1=5)

1. What does I in the restriction enzyme named 'Hin S2 I' indicate?
(a) It cuts after the first nucleotide in the restriction site.
(b) It is the first enzyme isolated from strain S2 of the bacterium.
(c) There is definitely more than one enzyme isolated from the same bacterium.
(d) There is only one enzyme that can be used to digest a plasmid from strain S2 for
the bacterium.
2. Number of bacterial strains from which restriction enzymes have been isolated.
(a) 230 (b) 250 (c) 200 (d) 220
3. Which vector can clone only a small fragment of DNA?
(a) Bacterial artificial chromosome (b) Yeast artificial chromosome
(c) Plasmid (d) Cosmid
4. Which of the following statements are correct with respect to a bioreactor?
I. It can process small volume of culture.
II. It provides optimumtemperature, pH, salt, vitamins and oxygen.
III. Sparged stirred-tank bioreactor is a stirred type reactor in which air is bubbled.
Choose the correct option.
(a) I and II (b) I and III (c) II and III (d) I, II and III
5. Assertion (A): Biotechnology deals with techniques that use living organism to
produce products useful for humans.
Reason (R): It uses only a unicellular organism.
(a) If both A and R are true and R is the correct explanation of A
(b) If both A and R are true, but R is not the correct explanation of A
(c) If A is true, but R is false (d) If A is false, but R is true
6. Assertion (A) :Use of chitinase enzyme is necessary for isolation of DNA from fungal
cells.
Reason (R): Fungal cell wall is made up of chitin and chitinase is able to digest it.
(a) If both A and R are true and R is the correct explanation of A
(b) If both A and R are true, but R is not the correct explanation of A
(c) If A is true, but R is false (d) If A is false, but R is true
II. ANSWER ALL THE QUESTIONS : (2x2=4 )
7. Explain with the help of a suitable example the naming of a restriction
endonuclease.
8. (a) Why and how bacteria can be made ‘competent’?
(b) How can retroviruses be used efficiently in biotechnology experiments inspite of
them being disease causing?
III. ANSWER ALL THE QUESTIONS : (2x3=6)
9. With the help of a schematic diagram only, show in three steps, the formation of
recombinant DNA by the action of restriction endonuclease –EcoR1 and DNA ligase.
10. Study the diagram given below and answer the following questions.

a) Why have DNA fragments in band D moved farther away in comparison to those
in band C?
b) Identify the anode end in the diagram.
c) How are these DNA fragments visualised?
d) How the DNA is separated for use in constructing recombinant DNA?

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IV. Answer all the questions : (1x4=4)
11. Shown below is a cloning vector 'Z' that Kamla wants to use to create a recombinant
vector with her gene of interest.

The vector consists of sites for three restriction enzymes - ScaI, HindIII and EcoRI.
Restriction sites for the same enzymes are also present in the gene of interest. There
are two 'ori' sequences - one allows it to replicate in Escherichia coli and another
allows replication in Zymomonas mobilis. Apart from this, the vector consists of two
antibiotic resistance genes - one against tetracycline (tet) and another against
chloramphenicol (cml).
a) What is the advantage of having two 'ori' sequences in the same vector? Give a
situation in which this would be particularly useful.
OR
a) If the vector was cut using HindIII, what would colonies growing on a medium
containing tetracycline definitely contain?
b) Suggest which of the three restriction enzymes would be suitable for insertion of
the gene of interest. Give two advantages of using the enzyme chosen by you.
c) State one disadvantage of using the other two restriction enzymes not chosen in (b).

V. Answer all the questions : (1x5=5)

12. (a) Explain briefly the process of PCR with a diagram (3M)
(b) Draw a well-labeled diagram of a simply stirred tank bioreactor. (2M)

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