Unit- IV (Biotechnology)

Maximum Marks: 70 Time: 3 hours

SECTION-A
1. Tools of recombinant DNA technology is:
(a) Restriction enzyme (b) Cloning vector (c) Competent host (d) All of these
2. First recombinant organism was:
(a) E. coli containing antibiotic production gene (b) Salmonella containing antibiotic production gene
(c) E. coli containing antibiotic resistant gene (d) Salmonella containing antibiotic resistant gene
3. Techniques of genetic engineering by which phenotype of organism can be changed, include:
(a) Formation of recombinant DNA (b) Gene cloning (c) Gene transfer (d) All of these
4. First recombinant DNA was constructed by:
(a) Stanley and Iwanosky (b) Stanley Cohen and Herbert Boyer (c) Jacob and Monad (d) H.G. Khorana and
Holley
5. Process of recombinant DNA technology includes:
(a) Isolation of DNA (b) Insertion of recombinant DNA
(c) Obtaining the foreign gene product and downstream processing (d) All of the above
6. Process involve in PCR is:
(a) Denaturation (b) Annealing (c) Extension (d) All of these
7. Ti-plasmids are present in:
(a) Agrobacterium (b) Retrovirus (c) E.coli (d) All bacteria

8. Bacillus thuringiensis produce a protein that kill:

(a) Lepidopterans (b) Coleopterans (c) Dipterans (d) All of these
9. Transgenic animals helps human kinds by:
(a) Study of normal physiological and development (b) Study of disease and vaccine safety
(c) Obtaining biological products like human proteins (α-1-antitrypsin) (d) All of the above
10. Most effective treatment for ADA deficiency patient is:
(a) Bone marrow transplantation (b) Functional ADA injected in patient
(c) Functional ADA cDNA introduces into lymphocyte from blood and infuses to blood of patient
(d) Functional ADA cDNA introduces in cell of early embryo and it infuse in patient
11. Toxin control cotton bollworm are produce by which Bt toxin genes:
(a) Cry IAb (b) Cry IAc (c) Cry IIAb (d) Both (b) and (c)
12. In RNAi, genes are silenced using:
(a) ssDNA (b) dsDNA (c) dsRNA (d) ssRNA
Question No. 13 to 16 consist of two statements – Assertion (A) and Reason (R). Answer these questions selecting the
appropriate option given below:
A. Both A and R are true and R is the correct explanation of A.
B. Both A and R are true and R is not the correct explanation of A.
C. A is true but R is false.
D. A is False but R is true.
13.
Assertion: Alternation of DNA or RNA is responsible for the genetic engineering.
Reason: Bioprocess engineering involve the maintenance of sterile i.e., microbial contamination free culture.
14.
Assertion: PCR is responsible for the formation of multiple copies of the gene of interest.
Reason: PCR is performed with the help of RNA polymerase enzyme.
15.
Assertion: Biotechnology is providing the best catalyst in the form of improved microbes or pure enzyme.
Reason: Biotechnology is creating optimal conditions through engineering for a catalyst to act.
16.
Assertion: Transgenic mice are used in the treating of vaccine safety.
Reason: Mice and human beings are the members of class mammalia.
SECTION-B
17. How sticky ends formed on a DNA strand? Why are they called so?
18. Name two commonly used bioreactors. State the importance of using a bioreactors.
19. How Rosie is considered different from a normal cow? Explain.
20. State the principal underlying gel electrophoresis and mention the two application of this technology in biotechnology.
21. Following is the diagrammatic representation of application of recombinant
DNA technology. Answer the following questions:
a. Pin point the correct name of the labels X and Y respectively
b. Which institute developed Humulin for the control of diabetes?
22. Define the following terms:
a. Biopiracy
b. Biopatent

SECTION-C
23. Biotechnology has helped farmers to get pest resistant cotton crops. Explain the technique adopted along with its
mode of action.
Or
a). Draw the figure of vector pBR322 and label the following:
Origin of replication
 Ampicillin resistance site
Tetracycline resistance site
Bam H1 restriction site
b). identify the significance of origin of replication.
24. A). Given below is a single stranded DNA molecule. Frame and label its sense and antisense RNA molecule.
5’ ATGGGGCTC 3’ sense
b). how the RNA molecules made from above DNA strand help in silencing of the specific RNA molecules?
25. Rajesh was doing gel electrophoresis to purify DNA fragments. Given below is the sketch of the observations of the
experiment performed by him.
a). At which end he would have loaded the samples and where?
b). Analyse the reason for different positions taken up by the DNA bands.
c). Elaborate the step he would have followed to visualize DNA bands. Cathode Anode

26. Name the genes responsible for making Bt cotton plants resistant to bollworm attack.
How do such plants attain resistance against bollworm attacks? Explain.
27. A). A recombinant vector with a gene of interest inserted within the gene of galactosidase enzyme is introduced into a
bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
b). Why is this method of selection referred to as insertional inactivation?
28. What is cry proteins? Name an organism that produced it. How has man exploited this protein to his benefit?
29. Name two cloning vectors. Describe the features required to facilitate cloning into a vector.
30. A). Explain with the help of a suitable example the naming of restriction endonuclease.
b). Write the sequence of DNA bases that the enzyme EcoRI recognises. Mention the point at which the enzyme makes a
cut in the DNA segment.
or
Mention any six fields of applications of biotechnology for human welfare.
SECTION-D
31. (a)What are molecular scissors? Give an example.
(b). Explain its role in rDNA technology with the help of an example.
Or
(a). Describe the characteristics of cloning vectors.
(b). Why DNA cannot pass through the cell membrane? Explain.
(c). How does an alien DNA gain entry into a plant cell by biolistic method.
32. Draw a labelled diagram of a simple strirred –tank bioreactor and describe its functioning.
Or
a. Explain the process of RNAi in tobacco plant.
 b. Describe the responsibility of GEAC, set up by the Indian Government.
33. a. Name the parts that are used to grow plants in laboratory by tissue culture technique.
b. What is micropropagation?
c. How you can produce healthy plants from virus infected plants?
d. What is protoplast? How they are helpful to produce somatic hybrids?
SECTION-E
34. Gene therapy is one of the applications of biotechnology to treat a person born with a hereditary disease.
a). What do you mean by the term gene therapy?
b). Name the hereditary disease for which the first clinical gene therapy was used?
c). Write down the steps of gene therapy involved to treat this disease?