MOCK TEST SERIES (BIOLOGY)-6

TIME= 1hr Full Marks= 200 (50 MCQs)

1. Biolistics (gene gun) is suitable for

(a) introducing rDNA into plant cells
(b) introducing rDNA into animal cells
(c) disarming the pathogen vectors
(d) DNA fingerprinting.

2. In genetic engineering experiments, restriction enzymes are used for

(a) viral DNA
(b) bacterial DNA
(c) eukaryotic DNA
(d) any type of DNA.

3. The DNA fragments produced by the use of restriction endonucleases can be separated by
(a) polymerase chain reaction
(b) gel electrophoresis
(c) density gradient centrifugation
(d) any of the above.

4. Plasmids in bacterial cells are

(a) extra-chromosomal DNA, which cannot replicate
(b) extra-chromosomal DNA, which can . self-replicate
(c) extra DNA associated with the genome
(d) extra DNA, associated with the genome, but cannot replicate.

5. The DNA polymerase enzyme used in PCR is obtained from

(a) Thermus aquaticus
(b) Escherichia coli
(c) Agrobacterium tumefaciens
(d) Salmonella typhimurium.

6. While isolating DNA from bacteria, which of the following enzymes is not used?
(a) Lysozyme
(b) Ribonuclease
(c) Deoxyribonuclease
(d) Protease
7. Significance of ‘heat shock’ method in bacterial transformation is to facilitate
(a) binding of DNA to the cell wall
(b) uptake of DNA through membrane transport proteins
(c) uptake of DNA through transient pores in the bacterial cell wall
(d) expression of antibiotic resistance gene

8. Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
(a) Denaturation of template DNA
(b) Annealing of primers to template DNA
(c) Extension of primer end on the template DNA
(d) All of the above

9. Which of the given statement is correct in the context of observing DNA separated by agarose gel
electrophoresis?
(a) DNA can be seen in visible light
(b) DNA can be seen without staining in visible light
(c) Ethidium bromide stained DNA can be seen in visible light
(d) Ethidium bromide stained DNA can be seen under exposure to UV light

10. ‘Restriction’ in Restriction enzyme refers to [NCERT Exemplar]

(a) cleaving ofphosphodiester bond in DNA by the enzyme
(b) cutting of DNA at specific position only
(c) prevention of the multiplication of bacteriophage in bacteria
(d) all of the above

11. The role of DNA ligase in the construction of a recombinant DNA molecule is (a) formation of
phosphodiester bond between two DNA fragments
(b) formation of hydrogen bonds between sticky ends of DNA fragments
(c) ligation of all purine and pyrimidine bases
(d) none of the above

12. In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells.
The host cells are then cultured on a medium containing ampicillin. The result will be
(a) both transformants and non-transformants cannot survive.
(b) both transformants and non-transformants can survive.
(c) transformants only and not the non-transformants can survive.
(d) transformants cannot survive, but non-transformants can not.

13.Which technique made it possible to genetically engineering living organisms ...

a) Recombinant DNA techniques
b) Heavy isotope labelling
c) X-ray diffraction
d) Hybridisation

14.There is a restriction endonuclease called EcoRI. What does 'co' part of it stands for ...
a) Coenzyme
b) coli
c) Colon
d) Coelom
15.Bt cotton genes repel ...
a) Bactreial pathogens
b) Fungal pathogens
c) Nematode parasites
d) Insect pest

16.GMO technology is useful for ..

a) Enhancing nutritional value of food
b) Helping to reduce post-harvest losses
c) Making crop more tolerant to abiotic stresses
d) All the above

17.The genetic defect adenosine deaminase (ADA) deficiency may be cured permanently by
a) Introducing bone marrow cells producing (ADA) into cells at an early embryonic stages
b) Administrating adenosine deaminase activators
c) Periodic infusion of genetically enginerred lynphocytes having functional ADA cDNA
d) Enzyme replacement therapy

18.Bt gene is got from ...

a) Brassica napus
b) Bacillus thuringiensis
c) Azolla
d) Rhizobium

19.Which is obtained from genetic engineering ..

a) Glucose
b) Haemoglobin
c) Golden Rice
d) None of the above

20.Restriction endonuclease is employed for cutting ...

a) A single strand DNA
b) Double strand DNA
c) RNA fragment
d) mRNA

21.Production of human protein in bacteria by genetic engineering is possible because

a) Human chromosomes replicate in bacterial cell
b) Mechanism of gene regulation is identical in humn and bacteria
c) Bacterial cell can undertake RNA splicing
d) Genetic code is universal

22.Microparticles for coating with DNA to be bombarded with gene gun are made of
a) Gold or tungsten
b) Platinum or zinc
c) Silver or platinum
d) Silicon or platinum
23.Some of the characteristics of Bt cotton are ...
a) Medium yield, long fibre and resistance to bittle pests
b) High yield and production of toxic proteins ceystals which kill dipteran pests
c) High yield and resistance to bollworms
d) Long fibre and resistance to aphids

24.Clot buster obtained from Streptococcus and modified by genetic engineering is ...
a) Streptokinase
b) Penicillin
c) Cyclosporin A
d) Statins

25.Maturation of proinsulin into insulin takes place after ..

a) Joining of c-peptide
b) Removal of c-peptide
c) Removal of disulphide bridge
d) Addition of disulphide bridge

26.Restriction enzymes are also called ..

a) Molecular makers
b) Vectors
c) Carriers
d) Molecular scissors

27.Polymerase chain reaction employs ...

a) Primers and DNA ligase
b) DNA ligase only
c) DNA plymerase only
d) Primary and DNA polymerase

28.Genetic engineering has helped in production of

a) Thyroxine
b) Insulin
c) Parathormone
d) Epinephrine

29.Which can be used as vector for transfer of DNA segment ... A) Bacterium B) Plasmid C) Plasmids
D) Nucleotides
a) A, B and C
b) A only
c) A and C
d) B and D

30.In genetics engineering which is used for transfer of genes from one cell to another ...
a) Vector
b) Probe
c) Plasmid
d) Virus
31.Recombinant DNA or rDNA technology as discovered by ..
a) Khorana
b) Bateson and de Vries
c) Sutton and Avery
d) Cohen and Boyer

32.What is true of Bt toxin?

a) The concerned bacillus has antitoxins
b) The inactive protoxin get converted into active form in the insect gut
c) Bt protein exists as active toxin in bacillus
d) The activated toxin enters the ovaries of the pest to sterilize it an thus prevents its multiplication

33.Thermostable enzymes 'Taq and Pfu' isolated from thermophilic bacteria are ..
a) RNA polymerases
b) DNA polymerases
c) DNA ligases
d) Restriction endonucleases

34.Which one of the following is used in genetic engineering?

a) Restriction endonuclease
b) RNA polymerase
c) DNA polymerase
d) Nuclease

35.Which is correct match of phenomenon and its explanation ...

a) Central dogma - RNA → DNA → Protein → RNA
b) Reverse transcription - PCR - Many coies of DNA sequence
c) Transcription - Formation of RNA and proteins
d) RNA silencing - Use of dsRNA

36.A patient brought to hospital with myocardial infarction is immediately given ...
a) Statins
b) Streptokinase
c) Penicillin
d) Cyclospoirin-A

37.Main objective of production of herbicide resistant GGM crops is to

a) Encourage ecofrindely herbicides
b) Reduce herbicide accumulation in food articles for health safety
c) Eliminate weeds from fields without the use of manual labour
d) Eliminate weeds from fields without the use of herbicides

38.First genetically modified plant commercially released in India is ...

a) Golden Rice
b) Bt Bringal
c) Slow ripening Tomato
d) Bt cotton
39.GAATTC is recognition site of restriction endonuclease .
a) Hind III
b) CecoRI
c) Bam I
d) Hae III

40.The genes crylAb and cryllAb produce toxins against _______ and _______, respectively.
(a) cotton bollworms, com borer
(b) nematode, cotton bollworm
(c) com borer, cotton bollworm
(d) com borer, nematodes

41. Which among the following is based on antigen-antibody interaction?

(a) PCR
(b) Electrophoresis
(c) ELISA
(d) All of these.

42. Which among the following is not allowed to take place in the case of RNA interference employed in
making tobacco plants resistant to the nematode, Meloidegyne incognitia?
(a) Transcription of mRNA
(b) Translation of mRNA
(c) Replication of DNA
(d) Maturation of hn RNA.

43. Night blindness can be prevented by use of

(a) golden rice
(b) transgenic tomato
(c) transgenic maize
(d) Bt brinjal.

44. The Ti plasmid used for producing transgenic plants is found in

(a) Azotobacter
(b) Rhizobium
(c) Azospirillum
(d) Agrobacterium

45. a-1 antitrypsin is:

(a) an antacid
(b) an enzyme
(c) used to treat arthritis
(d) used to treat emphysema.

46.. The trigger for activation of toxin of Bacillus thuringiensis is

(a) acidic pH of stomach.
(b) high temperature.
(c) alkaline pH of gut
(d) mechanical action in the insect gut.

47. In RNAi, genes are silenced using [NCERT Exemplar]

(a) ss DNA
(b) ds DNA
(c) ds RNA
(d) ss RNA

48. C-peptide of human insulin is [NCERT Exemplar]

(a) a part of mature insulin molecule
(b) responsible for formation of disulphide bridges
(c) removed during maturation of pro-insulin to insulin
(d) responsible for its biological activity.

49. Silencing of a gene could be achieved through the use of [NCERT Exemplar]
(a) short interfering RNA (RNAi)
(b) antisense RNA
(c) by both
(d) none of the above.

50. ELISA is

a. Using radiolabelled second antibody

b. Usage of RBCs
c. Using compliment mediated cell lysis
d. Addition of substrate that is converted into a coloured end product