Nand Foundation Academy, Shegaon.

9834274427
MH-CET CELL
Biology
Total Questions : 25 Total Marks : 25
Roll No.
24-Mar-2020
XII - A Div 10:00 AM To 12:00 PM
This is to certify that, the entry of Roll No. has been correctly written and verified.

Candidate’s Signature MH-CET Biology Invigilator’s Signature

Note :
i) All Questions are compulsory
ii) Darken ( ) the appropriate circle(#) of the most correct option/response with black ball point pen.

Genetic Engineering and Genomics

1) Blood stains are found at the site of a murder. If

A) D Nase
DNA profiling technique is to be used for
identifying the criminal, which of the following
B) recombinant vaccine
is ideal for use?

A) Serum B) Erythrocytes C) TPA

C) Leucocytes D) Platelets D) TGF-B

2) Alec Jeffreys developed the DNA fingerprinting 5) A technology, which has found immense use in
technique. The probe he used was solving cases of disputed parentage, is

A) Ribozyme B) Sex chromosomes A) Polymerase chain reaction

C) SNP D) VNTR B) DNA fingerprinting

3) The human genome project (HGP) was initiated C) Monoclonal antibody production
in
D) Recombinant DNA technology
A) 1988 B) 1990 C) 1992 D) 1994
6) Which one of the following can help in the
4) The clot formation can be prevented by diagnosis of a genetical disorder?
treatment with.......... in gene therapy.
A) ELISA B) ABO blood group

C) PCR D) NMR

7) Hybridoma is a biotechnique, which involves

fusion of
 12) The basis of DNA fingerprinting is
A) B-cell with T-cell
A) The double helix
B) T-cell with spleen cell
B) Errors in base sequence
C) spleen cell with myeloma cell
C) Polymorphism in sequence
D) myeloma cell with B-cell

8) Probes, used in DNA fingerprinting, are initially D) DNA replication

13) Genomics does not include

A) Single stranded RNA
A) improve techniques for microbial production
B) Mini satellite
of methane

C) 19 base long oligonucleotide

B) improve sustainable agricultural practices

D) All of the above

C) production of GM crops
9) Molecular scissors are
D) None of the above
A) Restriction endonucleases
14) In India, research in genetic modification of
organisms and safety issues are controlled by
B) DNA polymerase
A) DBT B) IARI C) CSIR D) GEAC
C) DNA ligase
15) DNA fingerprinting refers to
D) RNA polymerase
A) Molecular analysis of profiles of DNA
10) The technique of DNA fingerprinting was samples
initially developed by
B) Analysis of DNA samples using imprinting
A) Ian wilmut device

B) HarGobind Khurana C) Techniques used for molecular analysis of

different specimens of DNA
C) Jacque Monod
D) Techniques used for identification of
D) Alex Jeffreys fingerprints of individuals
11) Restriction endonucleases are most widely 16) Which one of the following techniques has
used in recombinant DNA technology. They are helped to solve many mysteries involving
obtained from murders, robberies and rapes?

A) Bacteriophages A) Gene splicing

B) Bacterial cells B) Computer technology

C) Plasmids C) DNA fingerprinting

D) All prokaryotic cells D) Gene cloning

17) Human insulin is being commercially produced
A) Gene splicing
from a transgenic species of

B) Computer technology
A) Escherichia coli B) Mycobacterium

C) DNA fingerprinting
C) Rhizobium D) saccharomyces

18) In DNA fingerprinting technique.............probe is D) Gene cloning

used for hybridisation of DNA fragments.
22) Genetically engineered bacteria are being
employed for production of
A) double-stranded RNA

A) Thyroxine B) Human insulin

B) double-stranded non-radio active DNA

C) Cortisol D) Epinephrine
C) single-stranded radioactive DNA
23) First hormone prepared by genetic engineering
D) single-stranded radioactive RNA is
19) The transgenic animals are generally produced
A) Oxytocin B) Somatotropin
for all of the following EXCEPT

C) Adrenaline D) Insulin
A) Testing of chemical safety
24) More advancement in genetic engineering is
B) testing of vaccine safety due to

C) stimulation of pathogenicity A) restriction endonuclease

D) production of pharmacologically important B) reverse transcriptase

proteins
C) protease
20) DNA fingerprinting technique was first
developed by
D) zymase
A) Jeffreys, Wilson and Thien 25) Genetic engineering is related with

B) Boysen and Jensen A) Eugenics B) Euphenics

C) Schleiden and Schwann C) Euthenics D) All of these

D) Edward and Steptoe

21) Which one of the following technique has

helped to solve many mysteries involving
murders, robberies and rapes?
 Answer Sheet

Biology : Genetic Engineering and Genomics

Total Questions : 25 Total Marks : 25

1 C 2 D 3 B 4 C 5 B 6 C 7 D 8 B 9 A 10 D 11 B 12 C
13 C 14 D 15 A 16 C 17 A 18 C 19 A 20 B 21 C 22 B 23 D 24 A
25 B
 Solution Sheet

Biology : Genetic Engineering and Genomics

Total Questions : 25 Total Marks : 25

1) DNA fingerprinting is a technique to identify a 7) The two types of cells, i.e. myeloma cells and
person on the basis of person"s antibody producing cells, e.g. B-cells of spleen
DNA specificity. The technique is based upon are made to fuse in cultures. These fused cells
the fact that the DNA constitution are called as hybridomas.
of an individual carries some specific sequence
8) The probes used for DNA fingerprinting are
of nucleotides, which do not
usually prepared from
carry any information for protein synthesis.
minisatellite or microsatellite DNA.
From the given options, leucocytes are to be
used for identifying the criminal 9) Restriction endonuclease is the enzyme which
because they are nucleated, whereas recognizes a specific DNA base
erythrocytes are enucleated. sequence and cleavages both the strands of a
DNA at a particular site called
2) The technique of fingerprinting was initially
restriction site having palindromic sequence.
developed by Alec
So, restriction endonuclease are
Jeffreys. He used a satellite DNA as probe that
also called molecular scissors.
shows very high degree of
polymorphisms. It was called as Variable 10) DNA fingerprinting is a technique to identify a
Number of Tandem Repeats (VNTR). person on the basis of persons
DNA specificity. The technique was developed
3) The Human Genome Project (HGP) was
by Sir Alec Jeffreys(1964)
initiated in 1990. It was an international
at Leicester University, UK.
scientific research project with the goal
of determining the sequence of chemical 11) Restriction endonucleases are enzymes that
base pairs, which make up human DNA. produce internal cuts called cleavage
in DNA molecules only within or near those
4) TPA (Tissue Piasminogenic Activator) is
sites which have specific base
recombinant
sequences (recognition site). These are
protein obtained, which is used to prevent or
obtained from the bacterial cells.
reverse
Restriction enzymes were obtained from the
blood clots in patients.
bacterial cells. Restriction enzymes
5) DNA fingerprinting technique is very useful in were discovered due to and named after the
solving disputed parentage cases phenomenon of host restriction of
and forensic cases, DNA fingerprints are bacterial phages.
obtained from RFLP or VNTR
12) Polymorphism in sequence is the basis of DNA
(satellite DNA) analysis of blood, hair or other
fingerprinting.
materials found at the place of
crimes.
6) PCR is a technique, in which a small fragment
of DNA is rapidly cloned or
duplicated to produce multiple DNA copies.
Thus, it helps in the diagnosis of a
genetical disorder. This technique was
conceived by American biochemist
Kary B Mullis.
13) Genomics is the study of molecular
organisation
of genomes, their information contents and the
gene products they encode. Genomics can be
used in industries to improve techniques for
microbial production of methane and other
fuels,
to find out new pesticides, to improve
sustainable
agricultural practices, etc.
14) In order to control the safety issues regarding
the
use of GM organisms, Indian Government has
set
an organisation Genetic Engineering Approval
Committee (GEAC), which controls all
researches
in genetic modifications of organisms and other
safety issues.
15) Important for DNA fingerprinting are short
nucleotide repeats that ary in number
from person to person but are inherited. These
are Variable Number Tandem
Repeats or VNTRs and these can be recognized
only through molecular analysis of
DNA SAMPLES. Alec Jeffreys (1985, 86)
discovered this technique for the
first time.
16) DNA fingerprinting is a modern technique that
compares sets of DNA by locating
identical sequences of nucleotides. It is
oftening used to solve many mysteries
involving murders, robberies and rapes.
17) The two DNA sequences or genes were made to
fuse with plasmids of
Escherichia coli and later allowed to form
insulin chains.
18) In DNA fingerprinting technique, hybridisation
is done
with the help of a radiolabelled VNTR probe
(small
segments of single-stranded DNA which help to
detect
the presence of a gene of a long DNA
sequence).
19) The transgenic animals are produced for
vaccine
safety, stimulation of pathogenicity and
production
of pharma cologically important proteins.
20) The technique of DNA fingerprinting was
developed for the first time by
Alec Jeffreys (1985, 86) and his colleagues at
Leicester University in
UK.
21) DNA fingerprinting is a modern technique that
compares sets of DNA by locating identical
sequences of nucleotides. It is often used to
solve many mysteries involving murders,
robberies and rapes.
22) In 1983, an American company Eli Lilly
synthesized artificial insulin with the
help of plasmids of Escherichia coli. It was
named as humulin. Since
then, genetically engineered E. coli bacteria are
being used to produce human
insulin.
23) Insulin was the first hormone prepared by
genetic engineering within the
E. coli bacterium. Insulin is made up of two 20
and 30 chains of amino
acid residues. Two different E. coli bacterial
cultures were used to
produced each of the insulin chain, these were
then recovered from the bacterial
and separated by β-galactosidase and finally,
chemically joined to make
human insulin.
24) Endonucleases are enzymes that produce
internal
cuts called cleavage in DNA molecule. A class
of
endonucleases cleave DNA only within or near
those
sites, which have specific base sequences,
such
endonucleases are known as restriction
endonucleases
and sites recognised by them are calied
recognition sites.
These have played major role in advancement
of genetic
engineering.
25) Genetic engineering is related with euphenics.
Euphenics is the study
of improvement of human race by altering the
protein synthesis (by mRNA)
process in cell. It is also called medical
engineering.