PRACTICE PAPER

Subject: BIOLOGY (044) Maximum Marks :70

Class: XII

GENERAL INSTRUCTIONS:
1. This Question paper contains - five sections A, B, C, D and E. Each section is compulsory.
However, there are internal choices in some questions.
2. Section A has 12 MCQ’s and 04 Assertion-Reason based questions of 1 mark each.
3. Section B has 5 Very Short Answer (VSA)-type questions of 2 marks each.
4. Section C has 7 Short Answer (SA)-type questions of 3 marks each.
5. Section D has 2 source based/case based/passage based of 4 marks each with sub-parts.
6. Section E has 3 Long Answer (LA)-type questions of 5 marks each.

Q. No. QUESTIONS Marks

SECTION A
16 x 1 =16
This section comprises multiple choice questions (MCQs) and Assertion -
Reason questions of 1 mark each.
1. Commelina has:
(a) Only cleistogamous flower (b) O n l y c h a s m o g a m o u s f l o w e r s 1
(c) B o t h o f a b o v e (d) N o f l o w e r s
2. Which human chromosome has least gene? 1
(a) First (b) Second (c) Y chromosome (d) one of these
3. Homologous organs show which type of evolution? 1
(a) Divergent (b) Convergent (c) Linear (d) None of these
4. Which method is not used to detect cancer? 1
(a) Biopsy (b) CBC (c) MRI (d) Computed tomography
5. Heroin is commonly called: 1
(a) Cocaine (b) Morphine (c) Smack (d) None of these
6. Propionibacterium shermanii is used to produce: 1
(a) Curd (b) Swiss cheese (c) Butter (d) Roquefort cheese
Stage of differentiation in spermatogenesis is: 1
7.
(a) Spermiation (b) Spermatogenesis
(c) S p e r m i o g e n e s i s (d) None of these
1
8. Which of the following antibodies form innate immunity? 1
(a) IgE (b) IgD (c) IgM (d) IgG
9. A nitrogen fixing microbe associated with the fern Azolla in rice fields is: 1
(a) Frankia (b) Rhizobium (c) Spirulina (d) Anabaena
A student wants to develop a colony of E coli possessing plasmid pBR 322 1
10. which is sensitive to tetracycline. Which restriction site he should use to
insert a foreign DNA?
(a) Hind III (b) Sal I (c) PvuI (d) EcoRI
The maximum number of existing transgenic animals is of _____. 1
11.
(a) Fish (b) Mice (c) Cow (d) Pig
At which point in the graph shown below would there be zero population 1
growth (DN/Dt = 0)?

12.

(a) a (b) b (c) c (d) d

In question number 13 to 16, a statement of assertion (A) is followed by a
statement of Reason (R). Choose the correct answer out of the following
choices.
(a) Both (A) and (R) are true and R is the correct explanation of A.

(b) Both (A) and(R) are true but R is not the correct explanation of A.

(c) (A) is true but (R) is false.

(d) (A) is false but (R) is true.

Assertion: After bleeding phase of menstrual cycle proliferative stage starts. 1

13.
Reason: After bleeding phase repair and proliferation of endometrial lining
occurs.
Assertion: Proto-oncogenes are cellular genes required for normal growth. 1
14. Reason: Under certain conditions they lead to the oncogenic transformation
of the cell.

15. Assertion: Streptococcus thermophilus increases nutritional value of milk. 1

Reason: Milk has lesser vitamin content than curd and yoghurt.
Assertion: A network of food chains existing together in an ecosystem is 1
16.
known as a food web.
Reason: An animal like kite cannot be part of a food web.
SECTION B 5  2 = 10
This section comprises very short answer (VSA) type questions of 2 marks
each.
Why is earthworm considered a farmer’s friend? Explain humification and 2
17.
mineralisation occurring in a decomposition cycle.
OR

2
 Compare the two ecological pyramids of biomass given below and explain
the situations in which this is possible. Also, construct an ideal pyramid of
energy, if 200,000 joules of sunlight is available.

18. Why are conventional methods not suitable for the assessment of biodiversity 2
of bacteria?
19. Explain brood parasitism with the help of an example. 2

20. Suggest any two possible treatments that can be given to patient exhibiting 2
adenosine deaminase deficiency.
21. Towards which electrode DNA fragments move during gel electrophoresis? 2
Why?

SECTION C 7x3= 21
This section comprises short answer (SA) type questions of 3 marks each.

Which is the most commonly used bioreactor in biotech labs. Mention any 2
22. 3
important characters of this bioreactor.

Observe the figure given below and answer the questions:

23. 3

(a) Which processes is shown by A?

(b) Identify enzyme B and its source.
(c) State importance of above enzyme?
OR
(a) What is insertional inactivation?
(b) Why insertional inactivation is said to be better method to detect
recombinants, then use of selectable markers.

Given below is the flow chart for cell mediated immune response.
Invading pathogen

Macrophage presents antigen

24. 3

T-helper cells recognise antigen and produces T-lymphocytes memory cells

Killer T-cell suppressor cell Memory cells

3
 Answer the following questions on the basis of the flow chart:
(a) Name any two types of cells which act as ‘Cellular Barriers’ to provide
Innate Immunity in humans.
(b) Thymus of a new born child was degenerating right from birth due to a
genetic disorder. Predict its two impacts on the health of the child.
(c) How do cytokine barriers provide innate immunity in humans?
A particular species of wild cat is endangered. In order to save them from
25. 3
extinction, which is a desirable in situ or ex situ? Justify your answer and
explain the difference between the two approaches.
Given below is a diagrammatic representation of the experimental setup used
by SL Miller for his experiment:

26. 3

(a) Write the names of different gases contained and the conditions set for the
reaction in the flask.
(b) State the type of organic molecule he collected.
(c) Write the conclusion he arrived at.
Observe the figure given below and answer the questions:

27. 3

(a) Label parts a, b and c.

(b) What will happen if c will not work properly?
(c) Pollen grains of a large number of species can be stored in liquid nitrogen
or fossilised for years. Give suitable reason for the statement.
Answer the following questions:
(a) At which stage of menstrual cycle progesterone level remains highest?
28. (b) On 15 – 17-day level of LH remains very high. Which term is used for 3
this condition? What is the importance of this condition?
(c) What happens with the empty graafian follicle? What is the importance of
it?
SECTION D 4 x2= 8
This section comprises 2 case study-based questions of 4 marks each.
The nomenclature of the pBR322 plasmid is as stated below: p – Plasmid,
29. BR - Stands for Bolivar and Rodriguez who constructed this plasmid, 4
322 - Number given to distinguish this plasmid from the other plasmids
developed in the same laboratory.
4
 • Plasmid pBR322 has two resistance genes—ampicillin resistance
(ampR) and tetracycline resistance (tetR)—which are considered useful
for selectable markers.
• Plasmid pBR322 has a variety of unique recognition sites for restriction
endonucleases.
• Two unique sites—Pst I and Pvu I—are located within the ampR gene
and BamHI and SalI are within the tetR gene.

(a) Antibiotic resistant gene in a vector is called selectable marker’. State its
function.
(b) What is the importance of ori site shown in figure?
(c) What is the importance of rop site shown in figure?
Microbes are also used for commercial and industrial production of certain
chemicals like organic acids, alcohols and enzymes. Examples of acid
producers are Aspergillus niger (a fungus) of citric acid, Acetobacter aceti (a
bacterium) of acetic acid; Clostridium butylicum (a bacterium) of butyric acid
and Lactobacillus (a bacterium) of lactic acid. Yeast (Saccharomyces
cerevisiae) is used for commercial production of ethanol. Microbes are also
used for production of enzymes. Lipases are used in detergent formulations and
are helpful in removing oily stains from the laundry. You must have noticed
that bottled fruit juices bought from the market are clearer as compared to those
made at home. This is because the bottled juices are clarified by the use of
pectinases and proteases. Streptokinase produced by the bacterium
Streptococcus and modified by genetic engineering is used as a ‘clot buster’ for
removing clots from the blood vessels of patients who have undergone
myocardial infraction leading to heart attack. Another bioactive molecule,
30. 4
cyclosporin A, that is used as an immunosuppressive agent in organ-transplant
patients, is produced by the fungus Trichoderma polysporum. Statins produced
by the yeast Monascus purpureus have been commercialised as blood-
cholesterol lowering agents. It acts by competitively inhibiting the enzyme
responsible for synthesis of cholesterol.
(a) Which organisms has been commercialised as blood cholesterol lowering
agent?
OR
Sara faced myocardial infraction which led to a heart attack, at the time she was
rushed to the hospital. Which medicine should have been administered to her
at that time?
(b) Why bottled fruit juices bought from the market are clearer as compared to
those made at home?
(c) Name the enzyme used in detergent formulations and are helpful in
removing oily stains from the laundry.
5
 (d) How is acetic acid produced?
SECTION E 5 x 3 = 15
This section comprises long answer (LA) type questions of 5 marks each

Describe the three different ways by which natural selection can affect the
frequency of a heritable trait in a population.
OR
Observe the figure given below and answer all questions given below:

31. 5

(a) Which process is shown in above figure?

(b) What can be the possible reason behind degeneration of 3 megaspores out
of 4?
(c) The structure shown in figure C is called as 7 celled, 8 nucleated. Explain.
(d) What is the role of filiform apparatus shown in figure c?

How did Hershey and Chase establish that DNA is transferred from virus to 5
bacteria?
32.
OR
Ribosomes move along mRNA molecules and catalyse the assembly of amino
acids into protein chambers. What is the role of ribosomes during translation?
A cross between a normal couple resulted in a son who was haemophilic and
a normal daughter. In course of time, when the daughter was married to a
normal man, to their surprise, the grandson was also haemophilic.
(a) Represent this cross in the form of a pedigree chart. Give the genotypes of
the daughter and her husband.
(b) Write the conclusion you draw from the inheritance pattern of this disease.
OR
Identify a, b, c, d, e and f in the table given below:
Syndrome Cause Characteristics of affected Sex Male/
individual Female/ Both
33. 5
Down’s Trisomy of a (i), (ii) b
21

c XXY Overall masculine d

development

Turner’s 45 with XO e (i) (ii) f