KENDRIYA VIDAYALAYA SANGATHAN, RAIPUR REGION

SAMPLE PAPER (SESSION 2023-24)

CLASS –XII SUBJECT-BIOLOGY
MM – 70 TIME : 3 HOURS

General Instructions: (i) All questions are compulsory.

(ii) The question paper has five sections and 33 questions. All questions are
compulsory.
(iii) Section–A has 16 questions of 1 mark each; Section–B has 5 questions of 2 marks
each; Section– C has 7 questions of 3 marks each; Section– D has 2 case-based
questions of 4 marks each; and Section–E has 3 questions of 5 marks each.
(iv) There is no overall choice. However, internal choices have been provided in some
questions. Astudent has to attempt only one of the alternatives in such questions.
(v) Wherever necessary, neat and properly labeled diagrams should be drawn.

SECTION – A
1 Any grassland ecosystem of food chain comprising of four tropic level exist .Identify
the amount of correct energy that will be conserved at each of its trophic level

Level of food chain Energy conserved

A Primary consumer 30% of the second level
B Tertiary consumer 20% of the third level
C Producer 40% of the first level
D Secondary consumer 10% of the second level
2 cry IIAb and cry IAb produces toxins that control
a) Cotton bollworms and corn borer respectively
b) Cotton borer and cotton bollworms respectively
c) Tobacco budworms and nematodes respectively
d) Nematodes and tobacco budworms respectively
3 Use of vaccine and immunization programme are done in maximum health care center.
Out of the options given below select the correct infectious disease that are controlled
by this program
a) diphtheria and pneumonia
b) polio and tetanus
c) cancer and AIDS
d) Both a and b
4 Cranial capacity of approximate 1650cc is associated with which man during human
evolution?
a) Cro-Magnon man
b) Neanderthal
c) Homo erectus
d) Homo habilus
5 The correct statement with respect to thalassemia in human is
a) alpha thalassemia is controlled by single gene HBB
b) the gene for Alpha thalassemia is located on chromosome 16
c) beta thalassemia is controlled by two closely linked gene HBA 1 and HBA 2
d) in beta thalassemia the production of Alpha globin chain is affected
6 Having become an expert on gel electrophoresis you are asked to examine a gel for
colleague. Where would you find the smallest fragment of DNA?
a) Near the positive electrode farthest away from the wells
b) Near the negative,close to the Wells
 c) Near the top near the negative pole
d) Near the middle they tend to slow down after the first few minutes
7 The process by which organism with different evolutionary history evolve similar
phenotype adaptation in response to a common environmental challenge is called
a) Natural selection
b) Convergent evolution
c) Non random evolution
d) Adoptive radiation
8 Occasionally a single gene may express more than one effect. The phenomena are called
a) Multiple allelism
b) Mosaicism
c) Pleiotropy
d) Polygeny
9 Given below are the different ART techniques with their correct procedure

ART Procedure
in Vivo fusion of the gametes in the female reproductive track
fertilization
ZIFT zygote with more than 8 blastomeres is transferred into the
uterus
IUT zygote or early embryo with up to 8 blastomeres is transferred
into the fallopian tube
IVF collected gamete are made to form the zygote in the laboratory

Select how many technical correctly matched with their procedure

a) 3 b) 4
c) 2 d) 1
10 The patient admitted for myocardial infraction. Which of the following bioactive
molecule can improve his condition?
a) cyclosporine -A
b) Statins
c) proteases and pectinase
d) Streptokinase
11 Select the important goal of human genome project from the given option
1) Store the information for data analysis
2) Cloning and amplification of human DNA
3) Identify all the gene present in human being
4) Use of DNA information to trace human history

a) 1 and 2 b) 2 and 3
c) 1 and 3 d) 2 and 4
12 Aquatic plant having long and ribbon like pollen grains is
a) Vallisneria
b) Hydrilla
c) Eichhornia
d) Zostera
Question Nos 13 to 16 consists of two statements, Assertion (A) and Reason (R ).
Answer these question selecting the appropriate option given below:
a) Both A and R are true and R is the correct explanation of A.
b) Both A and R are true and R is not the correct explanation of A.
c) A is true but R is false.
 d) A is false but R is true
13 Assertion: (A) Hind II is a restriction enzyme
Reason: (R) It is responsible for the cleavage of the sequence
14 Assertion: (A) Hybrid are generally superior than their parents
Reason: (R) All the dominant trait are present in the hybrid
15 Assertion: (A) Smoking increases the carbon monoxide content in the blood which has
greater affinity to hemoglobin than oxygen
Reason: (R) Carbon monoxide form a stable bond with the hemoglobin and does not
allows binding of oxygen. It reduces concentration of haem bounded Oxygen and cause
oxygen deficiency in the body
16 Assertion: (A) Turners syndrome is generally not found in males.
Reason: (R) the disorder occur due to monosomy.
SECTION B
17 Beta galactosidase enzyme is considered better selectable marker. Justify the statement
18 Write the location and function of sertoli cell in humans?
19 Name and explain the processes earthworm and bacteria carry on detritus.
20 Following are the features of genetic code. What does each one indicate
a) Unambiguous codon b)degenerate codon
c) universal codon d) stop codon
21 A patient showed symptoms of sustained high fever, stomach pain, and constipation but
no blood clot in stools. Name the disease and its pathogen. Write the diagnostic test for
the disease. How does the disease get transmitted?
SECTION C
22 Alien species invasions has been a threat to biodiversity. Justify with the help of
suitable example. List any other three causes responsible for such a loss.
23 The ability of the body to fight against disease causing agent is called immunity. It is of
two type i.e. innate immunity and acquired immunity.
a) Describe any two situations in which preformed antibodies are given to a person.
What kind of immunization is it? Also, discuss its advantage.
b) Describe autoimmunity?
24 Name two end products of double fertilization in angiosperm. How are they formed?
Write their fate during the development of seed.
25 Why must is cell be made competent in biotechnology experiments? How does the cell
can be made competent? Also state the role of biolistic gun in biotechnology
experiments.
OR
What is GMO?What are the possible dangerous aspect of genetic engineering
26 a) Arrange the following hormones in the sequence of their secretion in a pregnant
woman. LH, FSH,Relaxin ,hCG
b) Mention their source and the function they perform.
27 Describe how the lac operon operates, both in the presence and absence of an inducer in
E coli.
28 Transcription is considered more complex in eukaryotes. Elaborate.
SECTION D
29 Enzyme Taq polymerase is extracted from a eubacterial microorganism Thermus
aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et
at, (1976). Taq polymerase successfully replaced the DNA polymerase from E coli that
was being used in PCR earlier and this shift revolutionized the PCR.

a) What is a primer and its importance in PCR?

b) Taq polymerase after its discovery replaced E coli DNA polymerase in PCR
 technique. Explain giving reasons why was the need felt for the change?
c) Write the importance of PCR as a diagnostic tool? Or what are the source of
nucleotides in PCR.?

OR
Transgenic organism (plant, animal or microbes) are the most valuable gift of
biotechnology as they carry desired genes. Transgenic plants are fulfiling the food
requirement of growing world Population. Biotechnology is concerned with application
of living cell or tissue or their components for the benefit of mankind, thus, in the other
word it is a 'biological technology' (a technology based on application of living
organism). It uses knowledge of microbiology, tissue culture, genetic engineering and
chemical engineering. It is one of the most important discipline of modern molecular
genetics.

a) Name the main technique involved in the production of transgenic plant

b) What is humulin?
c) How is the conventional variety of cotton different from Bt cotton variety?
Or
Why do the toxic insecticidal proteins secreted by Bacillus thuringiensis kill the insect
and not the bacteria itself?
30 Pollution, climate changes, over exploitation by human etc, are responsible for changes
in the ecosystem. Change in an ecosystem is responsible for depletion in biodiversity
which directly or indirectly affect human being and their surroundings. So, it is
important to conserve the biodiversity. There is various method like in-situ
conservation, ex-situ conservation by which we can conserve our biodiversity.

a) How many biodiversity hotspots are identified globally?

b) What is the main cause of extinction of animal and plant
c) What do you mean by endemic species and where are they conserved?
Or Name any two summits that are organized to conserve biodiversity.
SECTION E
31 Three different type of crosses were made between plant P1 and P2 for different
characters. The F1 generation obtained is tabulated below. Mention the various laws of
inheritance associated with them.
Also find the phenotype ratio of F2 generation of each cross.

Cross Parent P1 Parent P2 F1 F2

1 YYRR (Yellow, yyrr (Green, YyRr (Yellow, -

round) wrinkled). round)
2 RR (Red) rr (White). Rr (Pink) -
3 TT (Tall) tt (Dwarf) Tt (All tall) -

OR
Answer the following questions based on Hershey and Chases’s experiments.
a) Name the kind of virus they worked with and why?
b) Why did they use two types of culture media to grow viruses in? Explain
c) What was the need for using a blender and later a centrifuge during their
experiments?
d) State the conclusion drawn by them after the experiments.
32 While teaching the concept of gametogenesis teacher told students that oogenesis and
spermatogenesis differ significantly. Teacher also told them that unlike spermatogenesis
oogenesis begins during the fetal life of female. However, in both the process LH and
FSH play a major role.
a) When and where does spermatogenesis begin in males?
b) Mention the effect of LH and FSH on spermatogenesis?
c) At which stage of cell division, oogonia are present in the female ovary during
birth?
d) When does the first and second polar body are formed during oogenesis?
e) How many spermatozoa and ovum are formed from one spermatogonia and
oogonia, respectively?
OR
Describe the embryonic development in human?
33 Describe the process of waste water treatment under the following head
a) Primary treatment
b) Secondary treatment
OR
a) Cancer is one of the most dreaded disease. Explain “contact inhibition” and
“metastasis” with respect to the disease.
b) Name the group of genes that have been identified in normal cells that could
lead to cancer. How do these genes cause cancer?
c) Name any two techniques that are useful in detecting cancers of internal organs.
d) Why are cancer patients often given alpha- interferon as part of the treatment?