GENERAL BIOLOGY 1

Name: Hanelore Damian Date: October 16, 2021

Strand, Gr. Level & Section: STEM 12- St. Hubert

ACTIVITY # 4.3
Analyzing Enzymatic Activity

I. Purpose: To analyze enzymatic activity in a given scenario.

II. Materials: Textbook, notes
III. Procedure:
1. Read and understand the scenario on your textbook pages 110-111.
2. Create a graph and analysis based on the results of the experiments.
a. Table A: Rate and enzyme Concentration
b. Table B: Rate and substrate Concentration
IV. Results and Interpretations

Figure 1: Rate and Enzyme Concentration Figure 2: Rate and substrate Concentration

Short description of figure 1 and 2:

The two figure uses line graph. Based on the graph of figure 1, there is three element; Lactose
concentration, Enzyme Concentration and Reaction Rate. The graph of the reaction rate continues going
up or rises. On the other hand, the graph of enzyme concentration slowly arises. And lastly, the graph of
lactose concentration remain constant. The graph of figure two namely Rate and Substrate
Concentration has also three elements. The blue line represents the lactose concentration, the orange
line represents the Enzyme Concentration and the gray line represents the line of reaction rate. As we
can see on the graph, the Enzyme Concentration remains the same. While the lactose concentration
continuously rises. Lastly, the graph of reaction rate continuously rises and remain at the end.

V. Conclusion:
In my conclusion, the rate of a chemical reaction increases as the concentration of the substrate
increases. Enzymes have the ability to greatly accelerate the rate of a reaction. When the substrate
concentration is high, however, enzymes become saturated.
VI. Guide Questions
1. What is the relationship between the reaction rate and the enzyme concentration?
The rate of a chemical reaction is defined as its speed. The reaction rate is proportional to the
concentration of the product. It is inversely proportional to the reactant concentration.The rate of a
reaction increases as the concentration of enzymes increases. As a result, thereaction rate is directly
proportional to the concentration of enzyme. As enzyme concentration rises, more reactants are
converted into products. As a result, a faster rate of reaction will be observed.

2. What is the relationship between the reaction rate and the substrate concentration?
The affinity of the enzyme for its substrate determines the relationship between rate of reaction and
concentration of substrate. This is usually expressed as the enzyme's Km (Michaelis constant), which is
an inverse measure of affinity. Km is the substrate concentration that allows the enzyme to achieve half
of Vmax. Because an enzyme with a high Km has a low affinity for its substrate, it requires a higher
substrate concentration to reach Vmax.