Supplementary table 2: Reported QTLs related to heat stress tolerance in rice (updated from Chen et al.

2020)

Year No. Parent typPopulationAppraisal HT index Chr. QTL/ LocuMarker intPhysical p

2003 1 HT AzucenDH Heading Spikelet fer 1 qhr1 RG381-RZ 30.87
3 qhr3-1 RZ892-RG5.18–12.90
4 qhr4-3 RG214-RG1 31.66–33.6
8 qhr8-1 RG978-RG17.44–21.6
11 qhr11-1 Z400-RG114.41–15.80
11 qhr11-2 RG118-Ad 4.41
2006 2 NipponbareBIL Heading Amylose co 6 qHTAC6 R2689-R19-
9 qHTAC9-1R1164-R16-
9 qHTAC9-2C506-G293-
Gel consist 4 qHTGC4 C1100-R17-
6 qHTGC6 L688-G200-
7 qHTGC7 C596-C213-
8 qHTGC8 G1073-R7219.31-20.6
10 qHTGC10 C1369-R18-
11 qHTGC11 G257-R728-
2007 3 HT KoshijiF6-RILs Ripening pWhite-back 1 qWK1-1 RM8068 1.66
1 qWK1-2 RM5501 34.55
2 qWK2 RM5916 34.06
8 qWK8 RM2680 0.15
2007 4 Hana-echizF2 White-back 3- RM4512 11.31
6- RM3034 4.25
Basal-back 6- RM3034 4.25
1000-kerne 3- RM4512 11.31
F3 White-back 4- RM3288 27.54
6- RM3034 4.25
White-back 6- RM3034 4.25
2008 5 HT T226/HF6-8 RILs Heading Seed-settin 3 qHt3 RM570-RM35.59–35.8
9 qHt9a RM108-RM18.81-19.3
12 qHt12 MRG0103- 26.11
2 qHt2 RM183-RM 25.14
3 qHt3 RM570-RM35.59-35.8
8 qHt8 RM547-MR18.81-19.3
9 qHt9b RM242-RM 26.11
2010 6 HT Bala/HF6-RILs Heading anSpikelet fer 1 qtl_1.1 B1065E10 38.35
2 qtl_2.2 C601 30.27
3 qtl_3.4 R1618/ 30.67
8 qtl_8.1 RIM2A/ -
8 qtl_8.2 G1073/ 20.53
8 qtl_8.3 RG598/ 27.6
10 qtl_10.1 C16 20.84
11 qtl_11.1 G1465 24.16
2010 7 W630/NippBC2F8 Heading anDays to hea 2- RM221-RM27.62-29.5
3- RM135-RM27.42-33.1
 8- RM3819-R3.02-4.38
10 - RM484-RM21.14-22.3
Beginning 5- RM249-RM10.78-19.9
10 - RM171-RM19.12-22.5
Median time 4- RM303-RM28.76-30.9
5- RM249 10.78
2011 8 HT 996/HSF9 RIL Heading Seed-settin 4 SSPF4 RM5687-R15.91-19.0
10 SSPF10 RM6132-R18.86-18.8
4 SSPC4 RM5687-R15.91-19.0
10 SSPC10 RM6132-R18.86-18.8
2012 9 HT N22/HSF2 Flowering Spikelet fer 1 qHTSf1.1 id1023892 39.55
4 qHTSf4.1 id4005120 17.69
9 qHTSF9.1 id9007356 22.44
11 qHTSF11.1id1100968 26.83
BC1F1 1 qHTSF1.1 id1024836 40.89
4 qHTSF4.2 id4011562 33.59
5 qHTSF5.1 id5001756 3.05
7 qHTSF7.1 id7005665 28.91
2012 10 Xiushui 09BC2F6 Heading anSpikelet fer 4 qSF4 RM518-RM2.03-13.16
6 qSF6 RM589-RM1.38-1.61
2014 11 Habataki/KCSSLs Ripening pAppearance 7 Apq1 Tak6166-3-
2015 12 PSB-Rc82 BC1F1,BCHeading anSpikelet fer 7- RM481 2.88
8- RM149 24.72
Pollen ferti 2- RM318 29.64
3- RM168 28.1
8- RM149 24.72
Start of fl 5- RM233B-R2.07-3.45
7- RM481 2.88
9- RM321 12.35
Peak of flo 5- RM233B 2.07
7- RM481 2.88
8- RM556 22.34
9- RM321 12.35
11 - RM4 0.93
2015 13 HT Giza17F2 Heading anSpikelet fer 1 qHTSF1.2 id1013342 23.33
2 qHTSF2.1 id2006486 16.08
3 qHTSF3.1 id3001137 2.06
4 qHTSF4.1
HT Giza17F2 6 qHTSF6.1 id6004481- 12.09
11 qHTSF11.2id11002639 8.66
IR64×MilyTestcross F2 1 qHTSF1.2 - 23.33
2 qHTSF2.2 - -
4 qHTSF4.1 - -
6 qHTSF6.1 - -
11 qHTSF11.3- -
2015 14 (NipponbarBILs Nature highAnther leng 1 qAL09-1 R1613-R193.44-4.54
2 Qal09-2 R1826 23.99
3 qal09-3 R2170 14.29
2 QAL10-2 C424 25.46
3 QAL10-3 R2170 14.29
 7 QAL10-7 C847 25.3
Apical dehi 5 QADL09-5R2558 13.06
11 QADL09-1S2137 8.3
5 QADL10-5R2558 13.06
8 QADL10-8R1813 7.59
Aasal dehis 3 QBDL09-3C25 3.96
8 QBDL09-8R202 24.18
9 QBDL09-9R79 11.8
2 QBDL10-2R26 22.16
8 QBDL10-8R202 24.18
Percentage 11 QLD10-11G44 (C3-C9.92-14.02
2016 15 HT HabatakCSSLs Anthesis stSpikelet fer 2 qSFht2 RM1234–R11.33-35.4
Pollen shed 4 qPSLht4.1 RM7585-Bb 22
Spikelet fer 4 qSFht4.2 RM3916–R 35.1
Pollen shed 5 qPSLht5 RM1248–R0.09-4.25
Daily flowe 3 qDFT3 RM3766–R6.93-25.11
8 qDFT8 RM5891–R24.62-28.2
10 qDFT10.1 RM6737–R18.78-23.0
11 Qdft11 RM1355– 17.67-25.1
Pollen shed 1 qPSLht1 RM1196– 21.90-31.5
7 qPSLht7 RM6394–R18.39-26.7
10 qPSLht10.2RM7492–R0.054-13.5
2017 16 HT ChikushF7-8 RILs Ripening pWhite-back 1 qWB1 RM7075 -
3 qWB3 RM4853 1.4
8 qWB8 SNP8_1 -
8 qWB8 RM3689 6.76
8 qWB8 RM72 19.34
Basal-back 2 qBW2 RM5470 27.15
3 qBW3 RM4853 1.4
6 qBW6 RM20429 25.43
12 qBW12 RM1986 21.25
1000-grain 2 qGW2 RM6933 29.31
2 qGW2 RM5470 27.15
3 qGW3 RM2791 4.57
3 qGW3 RM4853 1.4
8 qGW8 RM5556 4.59
8 qGW8 RM72 6.76
10 qGW10 RM2371 19.65
Days to he 3 qDTH3 RM4853 1.4
12 qDTH12 SNP12_1 -
2017 17 HT NaginaF8-RILs Flowering Spikelet ste 9 qSTIPSS9.1 16.75-17.1
12 qSSIPSS12.1 9.06-9.90
Yield per p 5 qSTIY5.1 25.45-25.7
3 qSTIY3.1 23.52–24.5
5 qSSIY5.1 25.45-25.7
2017 18 HT HabataCSSLs Booting staSpikelet fer 1 qHTB1 RM1387–R40.21-42.9
3 qHTB3-1 RM4108 0.54
3 qHTB3-2 RM5748–R12.33-22.3
3 qHTB3-3 RM3525–R30.39-32.4
4 qHTB4-1 RM7585–R0.23-13.08
 4 qHTB4-2 RM3534–R31.16-35.1
5 qHTB5-1 RM1248–R0.09-1.92
5 qHTB5-2 RM3236
6 qHTB6 RM5963–R8.82-11.07
10 qHTB10-1 RM3882–R2.74-11.74
10 qHTB10-2 RM3773–R19.97-23.0
11 qHTB11 RM286–RM 0.38-9.12
2017 19 HT Rejing3F2 - Empty grain 4 qHT4 RM17303-20.77-27.2
8 qHT8 RM6976-R23.56-27.9
12 qHT12 RM7376-R22.20-23.4
2018 20 HT LiaoyaRIL Heating andSeed-settin 1 qHTS1a RM11054-19.03-22.9
1 qHTS1b RM1231–R29.48-32.9
3 qHTS3 RM411–RM 21.43
4 qHTS4 RM471–RM 19.00-22.6
2018 21 HT N22/HSF8-RILs Seeding staroot length 5 rlht5.1 135.3-149. 28173385-
Root length 1 rlpc1.1 0-29.4 1860884-2
2 rlpc2.1 0-12.0 861429-91
3 rlpc3.1 130.8-137. 32129193-
4 rlpc4.1 0-7.5 992855-13
Shoot lengt 3 slht3.1 131.6–136.-
4 slht4.1 0–8.1 992855-13
6 slht6.1 142.4–156.32050861-
Shoot leng 2 slpc2.1 0–15.5 861429-91
4 slpc4.1 0–8.8 1357155-1
3 slpc3.1 70.3–98.8 17057110-
5 slpc5.1 38.1–55.5 5758641-6
6 slpc6.1 135.0–156.32050861-
10 slpc10.2 10.4–30.4 7677559-1
10 slpc10.3 11.7–32.2 7677559-1
2020 22 R53/HHT4BC5F2-4 Booting staSpikelet fer 1 qHTB1‑1 RM11633-30.54-30.5
2020 23 HT M9962/F2 Flowering Seed-settin 1 qSF1 - 34.28-34.4
2 qSF2 - 18.73-19.1
3 qSF3.1 - 26.12-26.2
3 qSF3.2 - 28.73-28.9
2021 24 Huanghuaz F2:3 Flowering Stage 8 qHTT8 3,555,000–4,520,000
Chen et al. 2020)

1
LOD Additive efPercentageReferences 2
10.2 -9.5 19.15 Cao et al, 2002 3
6.4 -6.46 2.86 4
6.4 4.33 1.28 5
5.2 5.03 1.73 6
7 8.49 4.94 7
10.7 10.37 22.81 8
7.29/15.18 1.4862543 26.18/50.1 Zhu et al, 2006 9
3.21 6.38 14.24 10
3.25 2.59 9.92 11
5.17 4.98 14.09 12
4.3/6.63 0.8873239 5.64/15.07 13
4.18 -3.69 7.74 14
8.57/6.1 7.37/4.00 30.86/19.14 15
7.79 -5.32 16.08 16
6.21 -3.84 17.64 17
3.4 -2.9 8.9 Tabata et al, 2007 18
5.6 3.6 15 19
3.8 2.8 9.3
3.6 2.8 9.2
4.6 -0.73 25.9 Asako et al, 2007
6.8 -0.84 31.2
8.08 -1.58 14.1
4.98 -0.17 12.3
4.36 -0.53 15.2
13.39 -1.14 59.6
7.8 -0.19 75.1
13.6 -5.2 11.4 Chen et al, 2008
5 3 7.6
5.3 -3.52 10
5.2 -4.87 7.4
7.1 -5.87 10.9
4.5 -4.73 7
6.3 6.16 12
6.2 -0.173 17.6 Jagadish et al, 2010
3.7 0.111 7.4
3.3 -0.136 11.4
3.4 -0.149 7.5
2.7 -0.107 7
3.4 -0.111 7.4
3.6 -0.146 13
3.2 0.146 8
4.11 2.77 5 Thanh et al, 2010
26.47 8.94 43.7
 11.41 5.5 19
6.84 3.9 8.8
5.43 0.42 27.2
4.83 0.48 28.6
3.04 0.29 14.7
3.39 0.37 20.5
13.63 9.11 21.3 Xiao et al, 2011
6.61 7.24 11.5
15.43 9.32 25.8
7.17 6.99 11.6
4.63 5.66 12.6 Ye et al, 2012
6.66 -0.81 17.6
2.43 -7.83 6.8
2.26 2.61 6.4
- - -
- - -
- - -
- - -
4.92 -11.92 10.33 Cheng et al, 2012
4.92 10.82 14.58
- - - Murata et al, 2014
- - - Baliuag et al, 2015
- - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
- - -
2.94 16.8 14.6 Ye et al, 2015
4.58 5.2 21.8
3.54 -9.7 17.3
4.06 16.5 19.5
4.81 17.2 20.6
3.43 14.2 15.2
5.16 4.1 13.3
4.8 10.4 12.5
5 11.7 13
4.45 6.7 11.6
5.38 8.3 13.9
4.27 -111.2 0.12 Tazib et al, 2015
7.78 132.8 0.23
4.32 -97.1 0.12
5.06 119.3 0.16
4.6 -113.6 0.15
 3.62 105.2 0.11
7.29 60.8 0.21
3.2 -44.7 0.11
3.16 46.2 0.11
4.08 47.6 0.14
3.46 41.6 0.13
3.75 -41.8 0.13
3.07 38.6 0.1
4.57 56 0.17
3.23 -46.2 0.11
5.51 -24.4 0.25
11.1 - - Zhao et al, 2016
14.7 - -
15.3 - -
10.2 - -
–26.5 - -
–18.1 - -
–25.6 - -
–29.1 - -
8- -
5.9 - -
9.1 - -
4.02 –3.82 15.6 Wada et al, 2015
8.9 -8.39 30.5
4.34 -1.44 18.5
3.3 -4.4 12.9
2.21 -4.45 7.4
2.93 –1.17 11
4.59 –1.41 17.9
2.78 1.4 10.7
3.35 1.6 12.9
2.29 -0.39 11.9
4.22 0.39 13.4
2.86 -0.32 13.6
6.78 0.48 20.5
6.92 0.61 31.5
6.44 0.58 27.5
2.79 -0.33 14.3
43.5 -3.98 87.5
3.96 -0.68 2.7
4.21 -1.25 16.1 Shanmugavadivel et al, 2017
3.88 -0.33 6.4
4.55 0.07 9
4.04 -0.08 6.5
3.51 -0.14 21.3
- 12.8 - Zhu et al, 2017
- 19.45 -
- -5.84 -
- 17.22 -
- 18.48 -
 - 14.33 -
- 14.22 -
- 9.98 -
- 9.37 -
- 11.96 -
- -6.28 -
- 6.31 -
2.78 -0.06 39.07 Zheng et al, 2017
2.56 0.11 11.74
3.14 0.04 19.14
3.61 3.87 5.47 Li et al, 2018
3.7 -3.22 8.02
3.85 4.01 11.4
10.17 -5.98 13.81
3.86 -0.04 20.4 Kilasi et al, 2018
3.95 -0.04 5.2
4.72 -0.05 7.5
6.83 -0.08 8.6
6.21 -0.06 8.3
5.57 -0.06 7
0.3 -0.05 5.3
4.18 -0.04 10.2
5.38 -0.12 19
4.96 -0.07 8.1
7.97 -0.05 8.6
5.75 0.03 6.6
7.75 -0.04 13.2
7.87 -0.2 17.1
5.69 0.13 17.9
- - - Cao et al, 2020
- - - Nubankoh et al, 2020
- - -
- - -
- - -
3,555,000–4,520,000 Chen et al, 2020
References
Baliuag NNA, Redoña ED, Hernandez JE et al (2015) Genetic analysis for heat tolerance and early morning flowerin
Cao Z, Li Y, Tang H, et al (2020) Fine mapping of the qHTB1-1QTL, which confers heat tolerance at the booting sta
Cheng L-Rui, Wang J-Min, Uzokwe V, et al (2012) Genetic analysis of cold tolerance at seedling stage and heat toler
Li M, Li X, Yu LQ, et al (2018) Identification of QTLs associated with heat tolerance at the heading and flowering st
Murata K, Iyama Y, Yamaguchi T, et al (2015) Identification of a novel gene (Apq1) from the indica rice cultivar ’Ha
Wada T, Miyahara K, Sonoda JY, et al (2015) Detection of QTLs for white-back and basal-white grains caused by hi
Xiao Y, Pan Y, Luo L, et al (2011) Quantitative trait loci associated with seed set under high temperature stress at the
Zhao L, Lei J, Huang Y, et al (2016) Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromo
Ye C, Tenorio FA, Argayoso MA, et al (2015) Identifying and confirming quantitative trait loci associated with heat
Tazib T, Kobayashi Y, Koyama H, Matsui T (2015) QTL analyses for anther length and dehiscence at flowering as tr
Zhu S, Huang R, Wai HP, et al (2017) Mapping quantitative trait loci for heat tolerance at the booting stage using chr
Cao, L. Y., Zhu, J., Zhao, S. T., He, L. B., and Yan, Q. C. (2002). Mapping QTLs for heat tolerance in a DH population from in
Thanh, P. T., Phan, P. D. T., Ishikawa, R., and Ishii, T. (2010). QTL analysis for flowering time using backcross population bet
Jagadish, S. V. K., Cairns, J., Lafitte, R., Wheeler, T. R., Price, A. H., and Craufurd, P. Q. (2010). Genetic analysis of heat tole
PS S, SV AM, Prakash C, et al (2017) High Resolution Mapping of QTLs for Heat Tolerance in Rice Using a 5K SNP Arra
Tabata M, Hirabayashi H, Takeuchi Y, et al (2007) Mapping of Quantitative Trait Loci for the Occurrence of White-Ba
Zhu, C. L., Jiang, L., Zhang, W. W., Wang, C. M., Zhai, H. Q., and Wan, J. M. (2006). Identifying QTLs for thermo-tolerance
Asako K., Bao G. L., Ye S. H., Katsura T. (2007). Detection of quantitative trait loci for white-back and basal-white kernels un
Chen Q. Q., Yu S. B., Li C. H., Mu T. M. (2008). Identification of QTLs for heat tolerance at flowering stage in rice, Sci. Agric
7/S00122-020-03539-7

8-017-0465-4

s://doi.org/10.1270/jsbbs.57.47