Professional Documents
Culture Documents
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9024
14. Y. Oyama, B. Matsushita, T. Fukushima, K. Matsushige, and A. Imai, “Application of spectral decomposition
algorithm for mapping water quality in a turbid lake (Lake Kasumigaura, Japan) from Landsat TM data,” ISPRS
J. Photogramm. 64(1), 73–85 (2009).
15. D. G. Bowers, K. M. Braithwaite, W. A. M. Nimmo-Smith, and G. W. Graham, “Light scattering by particles
suspended in the sea: the role of particle size and density,” Cont. Shelf Res. 29(14), 1748–1755 (2009).
16. V. Volpe, S. Silvestri, and M. Marani, “Remote sensing retrieval suspended sediment concentration in shallow
waters,” Remote Sens. Environ. 115(1), 44–54 (2011).
17. P. J. Werdell and S. W. Bailey, The SeaWiFS bio-optical archive and storage system (SeaBASS): current
architecture and implementation, Goddard Space Flight Center, Greenbelt, Maryland 20771 (2002).
18. M. H. Wang, S. H. Son, and W. Shi, “Evaluation of MODIS SWIR and NIR-SWIR atmospheric correction
algorithms using SeaBASS data,” Remote Sens. Environ. 113(3), 635–644 (2009).
19. J. L. Mueller and G. S. Fargion, Ocean optics protocols for satellite ocean color sensor validation, SeaWiFS
Technical Report Series, Revision 3 Part II (2002).
20. L. Gilpin and P. Tett, A methods for analysis of Benthic chlorophyll-a pigment, in: marine biology report
(Napier University Press, 2001).
21. N. A. Welschmeyer, “Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and
pheopigments,” Limnol. Oceanogr. 39(8), 1985–1992 (1994).
22. S. Tassan and G. M. Ferrari, “An alternative approach to absorption measurement of aquatic particles retained on
filters,” Limnol. Oceanogr. 40(8), 1358–1368 (1995).
23. Z. P. Lee, K. L. Carder, and K. P. Du, “Effects of molecular and particle scatterings on the model parameter for
remote-sensing reflectance,” Appl. Opt. 43(25), 4957–4964 (2004).
24. H. R. Gordon, O. B. Brown, R. H. Evans, J. W. Brown, R. C. Smith, K. S. Baker, and D. K. Clark, “A
semianalytic radiance model of ocean color,” J. Geophys. Res. 93(D9), 10909–10924 (1988).
25. A. Morel and B. Gentili, “Diffuse reflectance of oceanic waters. III. implication of bidirectionality for the
remote-sensing problem,” Appl. Opt. 35(24), 4850–4862 (1996).
26. C. D. Mobley, Hydrolight 6.0 user's guide, final report, SRI international, Menlo Park, Calif (2012).
27. A. Morel and L. Prieur, “Analysis of variances in ocean color,” Limnol. Oceanogr. 22(4), 709–722 (1977).
28. K. L. Carder, F. R. Chen, Z. P. Lee, S. K. Hawes, and J. P. Cannizzaro, MODIS ocean science team agorithm
theoretical basis document: case 2 chlorophyll a, ATBD 19, version 7 (2003).
29. G. Dall’Olmo and A. A. Gitelson, “Effect of bio-optical parameter variability on the remote estimation of
chlorophyll-a concentration in turbid productive waters: experimental results,” Appl. Opt. 44(3), 412–422
(2005).
30. G. Dall'Olmo, A. A. Gitelson, and D. Rundquist, “Towards a unified approach for remote estimation of
chlorophyll-a in both terrestrial vegetation and turbid productive waters,” Geophys. Res. Lett. 30(18), 1–4
(2003).
31. H. R. Gordon, “Radiometric considerations for ocean color remote sensors,” Appl. Opt. 29(22), 3228–3236
(1990).
32. Z. P. Lee, K. L. Carder, C. D. Mobley, R. G. Steward, and J. S. Patch, “Hyperspectral remote sensing for shallow
waters. 2. deriving bottom depths and water properties by optimization,” Appl. Opt. 38(18), 3831–3843 (1999).
33. C. D. Mobley, Light and Water: Radiative Transfer in Natural Waters (Academic Press, 1994).
34. M. Tzortziou, J. R. Herman, C. L. Gallegos, P. J. Neale, A. Subramanian, L. W. Harding, and Z. Ahmad,
“Determination of chlorophyll contentand tropic state of lakes using field spectrometer and IRS-IC satellite data
in the Mecklenburg Lake Distract, Germany,” Remote Sens. Environ. 73, 227–235 (2006).
35. A. Morel and H. Loisel, “Apparent optical properties of oceanic water: dependence on the molecular scattering
contribution,” Appl. Opt. 37(21), 4765–4776 (1998).
36. D. Doxaran, J.-M. Froidefond, S. Lavender, and P. Castaing, “Spectral signature of highly turbid waters,”
Remote Sens. Environ. 81(1), 149–161 (2002).
37. T. J. Smyth, G. F. Moore, T. Hirata, and J. Aiken, “Semi-analytical model for the derivation of ocean color
inherent optical properties: description, implementation, and performance assessment,” Appl. Opt. 45(31), 8116–
8131 (2006).
38. T. S. Moore, J. W. Campbell, and M. D. Dowell, “A class-based approach to characterizing and mapping the
uncertainty of the MODIS ocean chlorophyll product,” Remote Sens. Environ. 113(11), 2424–2430 (2009).
39. F. Gilbes, C. Tomas, J. J. Walsh, and F. E. Muller-Karger, “An episodic chlorophyll plume on the west florida
shelf,” Cont. Shelf Res. 16(9), 1201–1224 (1996).
40. A. A. Gitelson, J. F. Schalles, and C. M. Hladik, “Remote chlorophyll-a retrieval in turbid, productive estuaries:
Chesapeake Bay case study,” Remote Sens. Environ. 109(4), 464–472 (2007).
41. D. T. Yang, D. L. Pan, X. Y. Zhang, X. F. Zhang, X. Q. He, and S. J. Li, “Retrieval of chlorophyll a and
suspended solid concentrations by hyperspectral remote sensing in Taihu Lake, China,” Chin. J. Limnol.
Oceanogr. 24(4), 428–434 (2006).
42. H. R. Gordon and B. A. Franz, “Remote sensing fo ocean color: Assessment of the water-leaving radiance
bidirectional effects on the atmospheric diffuse transmittance for SeaWiFS and MODIS intercomparisons,”
Remote Sens. Environ. 112(5), 2677–2685 (2008).
43. R. C. Smith and K. S. Baker, “Optical classification of natural waters,” Limnol. Oceanogr. 23(2), 260–267
(1978).
44. C. D. Mobley, L. K. Sundman, and E. Boss, “Phase function effects on oceanic light fields,” Appl. Opt. 41(6),
1035–1050 (2002).
45. J. L. Mueller, C. O. Davis, R. A. Arnone, R. Frouin, K. L. Carder, Z. P. Lee, R. G. Steward, S. Hooker, C. D.
Mobley, and C. R. McClain, “Above-water radiance and remote sensing measurement and analysis protocols,”
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9025
Ocean Optics Protocols for Satellite Ocean-Color Sensor Validation, vol. Revision 4, III: Radiometric
Measurements and Data Analysis Protocols, pp. NASA Tech. Memo (2003).
46. L. Li, L. Li, K. Shi, Z. Li, and K. Song, “A semi-analytical algorithm for remote estimation of phycocyanin in
inland waters,” Sci. Total Environ. 435-436, 141–150 (2012).
47. W. J. Moses, A. A. Gitelson, S. Berdnikov, V. Saprygin, and V. Povazhnyi, “Operational MERIS-based NIR-red
algorithms for estimating chlorophyll-a concentrations in coastal waters — The Azov Sea case study,” Remote
Sens. Environ. 121, 118–124 (2012).
48. K. Suzuki, M. Kishino, K. Sasaoka, S. Saitoh, and T. Saino, “Chlorophyll-specific absorption coefficients and
pigments of phytoplankton off Sanriku, Northwestern North Pacific,” J. Oceanogr. 54(5), 517–526 (1998).
1. Introduction
By comparison to the open ocean, the coastal environment is a dynamic region where events
and processes operate over short temporal and spatial scales. Additionally, there are many
factors that influence the optical regime, e.g., tidal currents, variable wind and current
regimes due to diurnal heating and cooling, river discharge of colored dissolved organic
matter (gelbstoff), and suspended sediment, resuspension of bottom material from wave
action and storms events, and algal blooms from frequent, pulsed episodes of nutrient loading
[1]. These processes can rapidly alter the optical properties of coastal waters and their impacts
can be clearly observed by water color. The use of satellite imagery has proven beneficial for
rapidly assessing the optical biophysical variables in coastal environments dominated by
phytoplankton at temporal and spatial scales difficult to attain with direct field measurements
[2].
Models to estimate chla concentrations are commonly empirically or semi-analytically
based. Empirical approaches rely on a specific spectral feature, such as a spectral ratio
modeled to biophysical measurements using statistical regression. This kind of model is
simple and easy to implement [3]. However, it lacks a physical foundation and the
relationships are more geographically specific and cannot be applied other areas [4]. The
semi-analytical model containing the spectral optimization approach is based on solutions to
the radiative transfer equation [5]. These algorithms can be applied to different water types,
and estimation accuracy is often much better than that of empirical algorithms [6]. Because
this strategy relies on accurate spectral models for inherent optical properties of each
individual constituent presented in the water, however, these kinds of algorithm are generally
only appropriate to waters with characteristics similar to those used in the algorithm
development. Their applicability then can be quite limited and can result in significant errors.
More importantly, because of the wide variation of optical properties found for global waters,
one semi-analytical function cannot fit all waters, unless the waters are restricted to Case I
conditions where all optical properties co-vary with chla concentrations [7]. Thus, an accurate
semi-analytic model is still under development.
Recently, Gitelson et al. [8] outlined a three-band semi-analytical algorithm for estimating
chla concentration in turbid coastal waters, described how the algorithm works, analyzed the
influence factors of the algorithm, and suggested a method to optimize the band positions.
This model relates chla concentration to remote sensing reflectance in three spectral bands, so
the model independents the accurate information for the inherent optical properties in remote
sensing applications. The model involves three assumptions [8]: (1) the absorption by chla at
λ1 is very different from that at λ2, but absorption by suspended solids and gelbstoff at λ1 is
close to that at λ2; (2) reflectance at λ3 is minimally affected by the absorption of water
quality constituents and the total absorption at λ3 is a measure of the absorption by water; and
(3) the total backscattering coefficients of the three bands are approximately equal. The
algorithm for chla estimation has been well tested and evaluated using observations from
lakes and reservoirs with variable optical properties in Nebraska and Iowa, and it was
determined that the conceptual model may be considered as a unified approach for remote
quantification of absorbing constituents in a variety of systems with Case II waters [8, 9].
Dall'Olmo and Gitelson [10] found that the variability in the quantum yield chla
fluorescence and the chla specific absorption coefficient, among other factors, considerably
affect the accuracy of three-band algorithm in chla prediction. Instead of re-initialization of
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9026
the models for water bodies with specific optical properties, Dall'Olmo and Gitelson [10]
suggested tuning the spectral band positions in order to minimize these effects. Recently,
Chen et al. [11] have demonstrated that the three assumptions for the three-band algorithm
may be violated in turbid waters. The reasons can be attributed to [11, 12]: (1) the absorption
curves of water quality constituents were related exponentially to the wavelength at near-
infrared regions [13, 14], so it is difficult to detect two bands that meet the requirement of the
“spectrally flat” assumption; (2) the aquatic particle absorption significantly varies with the
type of suspended particles at near-infrared (NIR) and cannot be neglected in turbid coastal
waters [5, 15], so that the second assumption may be violated in such waters; and (3) the total
backscattering coefficient exponentially decrease with wavelength [15, 16], so that the third
assumption may fail to completely removing the impacts of backscattering on three-band
algorithm. Consequently, due to the limitations of these three assumptions, the desirable chla
absorption is still not perfectly isolated by three-band algorithm. It may produce an
unacceptable uncertainty while using three-band algorithm to retrieve chla concentration from
these waters with highly turbidity or spectrally unevenness of gelbstoff at NIR regions [6,12].
More local information or improved models are required to further optimize the three-band
algorithm.
The objectives of this study are to validate the performance of the three-band algorithm
and further improve it for coastal waters. The specific goals are: (1) to assess the applicability
of a three-band algorithm in turbid coastal waters; (2) to discuss the impacts of the limitations
of the three assumptions on three-band algorithm; (3) to develop an innovative modified
three-band algorithm and variation three-band algorithm for estimating chla concentration in
coastal waters; (4) to evaluate the accuracy of the variation three-band algorithm to accurately
estimate chla concentration in coastal waters; and (5) to compare the accuracy of the three-
band, modified three-band, and variation three-band algorithm in estimating chla
concentration from coastal waters.
2. Data, methods and techniques
2.1 Data sets used
Satellite ocean color missions require and abundance of high quality in situ measurements for
bio-optical and atmospheric algorithm development and post-launch product validation and
sensor calibration [17]. Since 1997, to facilitate the assembly of a global data set, NASA has
funded the collection of ocean in situ data for data product validation, algorithm development,
satellite data comparison and inter-calibration, and data merger studies and time series
analyses [18]. The Sea-viewing Wide Field-of-View Sensor Bio-optical Archive and Storage
System (SeaBASS) maintains a local repository of in situ ocean optical and bio-optical data to
support and sustain regular scientific analyses. Specifically, the database includes in situ
ocean optical, biological, and other related oceanographic data (see details in
http://seabass.gsfc.nasa.gov). These data were collected by various researchers around United
States and Europe, using various instrumentation with all measurements closely following
rigorous, community-defined deployment and data processing protocols [19].
The SeaBASS in situ data have been continuously used to support the SeaWiFS and
MODIS ocean color product validation and algorithm [17, 18]. Thus, the SeaBASS data are
appropriate for the new algorithm calibration and evaluations. To evaluate the accuracy of the
three-band, modified three-band, and variation three-band algorithms in predicting chla
concentration, two independent data sets containing the spectral optical properties, inherent
optical properties, and chla concentration of water column were used. These data sets (Fig. 1)
were collected by the NASA SeaWiFS project as the SeaBASS data set. The first data set was
used for model calibration, while the second one was used for model validation. The
calibration data set containing 151 samples was collected in 2000-2001 in West Florida Shelf,
USA, and the second data set including 52 samples was collected on 1999 and 2002, in West
Florida Shelf, USA. The laboratory analyses were carried out within 24 h following sample
collection. Chla concentration was extracted and measured with 90% acetone in accordance
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9027
with the Ocean Optical Protocols of NASA [19], a generally accepted method of quantifying
Chla concentration in chemical and biological fields [20, 21].The data for ap(λ) and ag(λ)
were measured according to Tassan and Ferrari [22] and the data for CDOM according to the
SeaWiFS protocols [19].
Fig. 1. Locations of field measurements, where green circle denotes the calibration data set and
the red star refers the validation data set.
where T = tutd/n2 with tu the radiance transmittance from below to above the surface, td is the
irradiance transmittance from above to below the surface, and n is the refractive index of
waters. κ is the water-to-air internal reflection coefficient, and Q is the ratio of upwelling
irradiance to upwelling radiance evaluated below the surface. For a nadir-viewing sensor and
remote sensing domain, Q, in general, ranges between 3 and 6 [25]. As Rrs(λ) is small for
most oceanic and coastal waters, the variation of the Q value has little effect on the
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9028
conversion between Rrs(λ) and rrs(λ) [7]. Based on calculated HYDROLIGHT Rrs(λ) and
rrs(λ) values, T≈0.52 and κQ≈1.7 for optically deep waters [26].
Remote sensing of water quality constituent’s concentration is based on the relationship
between rrs(λ) and the inherent optical properties, namely, the total absorption a(λ) and the
total backscattering coefficients bb(λ) [27]:
bb ( λ )
rrs ( λ ) ≈ γ (3)
a ( λ ) + bb ( λ )
where γ is unchanging with respect to wavelength and viewing geometric conditions [24, 25,
27]. The total absorption and scattering coefficients may be expanded as follows:
a ( λ ) = aw ( λ ) + a p ( λ ) + ac ( λ ) + ad ( λ ) (4)
where the subscripts “w”, “p”, “c”, and “d” refer to water, chla, colored dissolved organic
matters (CDOM) and inorganic particles, respectively. Due to the similarity in spectral shape,
the ac(λ) and ad(λ) are difficult to spectrally separate with the current absorption coefficients
decomposed model, expect for Case I conditions [28], so the ad(λ) term is combined
operationally with ac(λ), and both detritus and CDOM absorption are represented by gelbstoff
absorption ag(λ). the total absorption coefficient can be expressed as:
a ( λ ) = aw ( λ ) + a p ( λ ) + ag ( λ ) (5)
Gitelson et al. [8] suggested that reciprocal reflectance in the first spectral band r−1rs(λ1)
should be maximally sensitive to ap(λ1); However, in addition to absorption by chla, r−1rs(λ1)
is also affected by absorption of gelbstoff, inorganic particles, and water as well as
backscattering by all particulate matter. Fortunately, the effect of these factors can be
minimized using a second spectral band, where r−1rs(λ2) is minimally sensitive to absorption
by chla and absorption of gelbstoff and inorganic particles at λ2 is quite close to that in band
λ1. Thus, the difference r−1rs(λ1)-r−1rs(λ2) must meet the follow requirements:
1 a p ( λ1 ) + aw ( λ1 ) − aw ( λ2 )
rrs−1 ( λ1 ) − rrs−1 ( λ2 ) ≈ (6)
γ bb ( λ1 )
Unfortunately, the difference r−1rs(λ1)-r−1rs(λ2) is still affected by bb(λ1), i.e., if
backscattering varies between samples, the model output would be different for the same chla
concentration. To account for this, a third spectral band λ3 is adopted, where reflectance is
minimally affected by absorption of water quality constituents, i.e., a(λ3)~aw(λ3) and
a(λ3)>>bb(λ3). This means that λ3 has to be restricted within the NIR regions [8]. Thus, the
three-band algorithm can be approximated as follow [8, 29, 30]:
a p ( λ1 ) + aw ( λ1 ) − aw ( λ2 )
rrs−1 ( λ1 ) − rrs−1 ( λ2 ) × rrs ( λ3 ) ≈ (7)
aw ( λ3 )
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9029
s (λ ) l1s ( λ )
rrs ( λ ) = l 0 + (8)
s ( λ ) + 1 s ( λ ) + 1
here, s(λ) is the ratio of total backscattering coefficient to total absorption coefficient. For
nadir-viewed rrs(λ), Gordon et al. [24] found that l0≈0.0949 and l1≈0.0794 for Case I waters,
while Lee et al. [32] suggested that l0 of 0.084 and l1 of 0.17 work better for higher scattering
coastal waters. Actually the values of l0 and l1 may vary with particle phase function [5],
which is not known remotely. Without local bio-optical information or models to
predetermine the values of l0 and l1 in an semi-analytical algorithm, the Gordon et al. [24]
suggested values are used in Case I waters, while Lee et al. [32] determined values work in
Case II waters.
From Eq. (8),
−l 0 + l 02 + 4l1rrs ( λ )
s (λ ) = (9)
2l1 + l 0 − l 02 + 4l1rrs ( λ )
If considering non-negligible absorption and backscattering of suspended solids at NIR
regions, the violation “spectrally flat” assumption at λ1 and λ2 and the water optical properties
dependent γ in Eq. (3), the three-band should be written as,
a p ( λ1 ) + aw ( λ1 ) − aw ( λ2 ) + Δag ( λ1 ) γ ( λ2 )
rrs−1 ( λ1 ) − rrs−1 ( λ2 ) × rrs ( λ3 ) ≈ (10)
aw ( λ3 ) + ag ( λ3 ) + bbp ( λ3 ) γ ( λ1 )
where, X = [ag(λ2)/bb(λ2)]/[ag(λ1)/bb(λ1)]. Note that the value of X may vary based on the
nature of waters under study, such as humic versus fulvic acids and abundance of detritus
[33]. Local information or and improved algorithm is needed to reinitialize the X value in
typical remote sensing application.
2.5 variation three-band algorithm
Compared to the three-band algorithm, the three limitations have been reduced by the
modified three-band algorithm. These are: (1) the backscattering scattering of suspended
solids over NIR region in highly turbid waters; (2) violation of the “spectrally flat”
assumption in optical complicated waters; and (3) poor approximation of coefficient γ.
However, the modified three-band algorithm may still be violated in both turbid waters and in
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9030
most of the entire ocean, because this model is still limited in that: (1) non-negligible
absorption of suspended solids at λ3, e.g., Tzortziou et al. [34] showed that the absorption of
particulate matter in the 700-730nm region cannot be neglected in the Chesapeake Bay, while
Binding et al. [13] suggested that particle matter may be an important contributor to the total
spectral absorption signals in Lake Erie waters at red and NIR regions; and (2) non-negligible
water molecular backscattering at red and NIR regions (λ<800nm), except for highly turbid
waters, e.g., Morel and Loisel [35] indicate that the influence of molecular scattering by water
molecules is not negligible, leading to a gradual change in the shape of the phase function,
when the chla concentration is low enough (in most of the entire ocean), while Lee et al. [23]
show that the water molecular backscattering ranged from negligible to generally significant
for natural assemblages of global waters. Doxaran et al. [36] suggested that bbw(λ) was equal
to one-half of the total backscattering coefficient for the range 400-800nm and is negligible at
greater wavelengths in aquatic environments with low concentration of water quality
constituents.
Traditionally, the wavelength dependence of bbp(λ) can be modeled as
Y
551
bbp ( λ1 ) = bbp ( 551) (13)
λ
where Y is the exponential coefficient. Owing to the difference in spectral shape [15, 28], the
bbw(λ) term cannot be combined operationally with bbp(λ), so the ratio of bb(λ3) to bb(λ1) is
not constant and varies consistently with backscattering by particle matter. This limitation
may cause modified three-band algorithm to be violated in slightly turbid waters. To account
for this, a variation of the three-band algorithm was developed for estimating pigment
concentrations both in turbid and low chla concentration waters.
Because the s(λ) is the ratio of total backscattering to total absorption, backscattering by
particle matters can be isolated from product of s(λ)a(λ) as long as backscattering by water
molecules is known, i.e.,
s ( λ ) a ( λ ) = bbw ( λ ) + bbp ( λ ) (14)
When the analytical expression of backscattering coefficients is known (Eq. (13)), the
relationship of product of s(λ)a(λ) at λ1 and λ2 can be denoted by using:
where κ12 = (λ2/λ1)Y. To determine the total absorption coefficient using measurements of
s(λ), the spectral slope technique [37] can be used to determine the total absorption due to
water quality constituents. Starting from a general wavelength pair λ1 and λ2:
a ( λ1 ) = ζ 12a ( λ2 ) + ξ12 (16)
where ζ12 and ξ12 are semi-analytical coefficients, which have been either related to
concentration of water quality constituents or absorption at a wavelength. Substituting Eq.
(16) into Eq. (15), yields:
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9031
ξ13s ( λ1 ) − bbw ( λ1 ) − κ13bbw ( λ3 )
a ( λ3 ) = (18)
κ13s ( λ3 ) − ζ 13s ( λ1 )
where κ13 = (λ3/λ1)Y, ζ13 and ξ13 are semi-analytical coefficients, which have been either
related to water quality constituents’ concentration or absorption at λ1 and λ3. Several studies
have reported [7, 23, 38] that the absorption related to chla and gelbstoff at blue-green ranges
can be calculated using a spectral decomposed method, as long as total absorptions at two
wavelengths are known. However, at red-NIR ranges, the spectral decomposed method
produces poor accuracy in decomposing ap(λ) and ag(λ) from total absorption, because
ap(λ)<< a(λ) and ag(λ)<< a(λ), i.e., a small bias in a(λ) estimation may result in a large bias
in ap(λ) and ag(λ) retrieval. To overcome this limitation, an empirical spectral decomposed
method (ESDM) was used to retrieve ap(λ) from a(λ) in this study. The ESDM method is
expressed by:
a p ( λ3 ) = π 0a ( λ3 ) + π 1a ( λ2 ) + π 2 (19)
where π0, π1, and π2 are empirical coefficients, which are computed using non-linear robust
regression method suggested by Chen and Quan [6].
3. Results
3.1 Chla concentrations and water optical characteristics
Episodic blooms of toxic dinoflagellates have been reported as potential contributors to the
total primary production in the West Florida Shelf. During dinoflagellate bloom periods, chla
concentrations in surface waters vary from 2 to 30mg/m3, but chla concentrations during non-
bloom periods are <1mg/m3 [39]. By comparison, each of the data sets taken in West Florida
Shelf from 1999 through 2002 (Table 1a and Table 1b) contained both bloom and non-bloom
periods. Figure 2 shows ap(440) plotted against ag(440) in the West Florida Shelf, indicating
that the water optical properties in the West Florida Shelf are not only determined by chla and
covarying pigments, but are also determined by other water quality constituents such non-
covarying CDOM and non-algal particle, even though ap(440) and ag(440) are significantly
correlated in these data set (correlation coefficient 0.6931). Thus, the West Florida Shelf
water falls into Case II category.
Table 1. Descriptive statistics of the optical water quality parameters measured: chla
concentration, ad(440), ac(440), ap(440), a(440), and transparency (SD refers to standard
deviation).
a. Calibration data set taken from West Florida Shelf measured in 2000-2001, 151 samples
Min Max Median Average SD
[chla], mg/m3 0.2010 13.35 0.2619 0.6872 0.9330
ad(440), 1/m 0.0003 0.1397 0.0030 0.0097 0.0188
ac(440), 1/m 0.0049 0.3774 0.0272 0.0493 0.0556
ap(440), 1/m 0.0090 0.3876 0.0243 0.0494 0.0582
b. Validation data set taken from West Florida Shelf measured in 1999 and 2002, 52 samples
Min Max Median Average SD
[chla], mg/m3 0.2011 4.3769 0.7030 1.0019 1.9307
ad(440), 1/m 0.0012 0.1375 0.0100 0.0224 0.0287
ac(440), 1/m 0.0029 0.9876 0.0401 0.1329 0.2136
ap(440), 1/m 0.0094 0.4371 0.0448 0.0844 0.0964
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9032
1.2
y = 1.7639x - 0.015
1 2
R = 0.6931
a g(440) (m )
0.8
-1
0.6
0.4
0.2
0
0 0.1 0.2 -1 0.3 0.4
a p(440) (m )
Fig. 3. Remote-sensing reflectance spectra used in this study. (a) Calibration data set taken
over West Florida Shelf in 2000-2001; and (b) Validation data set taken over West Florida
Shelf in 1999 and 2002, respectively.
Multiple factors contribute to the reflectance patterns in aquatic environments. In the blue
range (400-500nm), absorption by geblstoff and SSC, and scattering by particulate matter
contributed strongly to reflectance patterns [33, 40], but reflectance did not have pronounced
spectral features for the broad range of turbidity and chla concentrations at all sites. Figure 4
shows a chla absorption peak around 440nm, so there must be a reflectance minimum near
440nm in spectral curves, but it was distinct only when the chla concentration was above
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9033
150mg/m3 [8]. Near 490nm another trough on reflectance curve is shown due to largely
carotenoids absorption [41]. In the green spectral ranges (500-600nm), absorption by
pigments was minimal and scattering by all particulate matter played the main role in
reflectance, and reflectance had a prominent peak around 575nm. An absorption peak around
675nm indicated a trough around 675nm corresponds to the red chla absorption maximum in
spectral curves. However, it was indistinct in West Florida Shelf water due to the low chla
concentration. A distinct peak located around 695nm,appeared in almost all samples. This
peak is the result of both high backscattering and a minimum in absorption by all water
quality constituents including pure water [8, 40].
0.3
a p(λ ) (m )
0.2
-1
0.1
0
400 500 600 700
Wavelength (nm)
Fig. 4. Chla absorption coefficient taken over West Florida Shelf in 1999-2002
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9034
[ap(λ) + ag(λ)] to total absorption in West Florida Shelf waters. By comparison, the
absorption by particulate matter is much smaller than total absorption or absorption by water
molecules at NIR band, e.g., ratio value no more than 4% at band position larger than 600nm.
Thus, the third band is set within 600 to 750nm. Finally, the non-linear recursive method is
used to calculate the RMS of [R−1rs(λ1)-R−1rs(λ2)] Rrs(λ3) versus [chla] within the bands setting
ranges given as previous. The results (Fig. 6) indicates that the three-band algorithm has the
optimal accuracy in case of λ1 = 620nm, λ2 = 712nm and λ3 = 730nm, whose correlated
coefficients are larger than 0.3885.
Fig. 5. Particulate matters’ absorption characteristics in West Florida Shelf waters. (a) RMS of
rrs(λ) linearly relating to ap(λ); and (b) Ratio of sum absorption by particulate matters to total
absorption.
Fig. 6. Scatter plot of estimated versus measured chla concentration of calibration data set
using two algorithms. (a) results from the three-band algorithm; and (b) results from the
modified three-band algorithm. Here, X and Y axis are the logarithmic coordinates.
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9035
linearly related to but not equal to Xap(λ2). This means that λ2 can also be set to 580 to
694nm. Thus, for the modified three-band algorithm, λ2 should be between 580 and 750nm.
The third one should be minimally sensitive to absorption both by chla and gelbstoff, so λ3
has to be restricted 600 to 750nm, which is same with the band ranges of three-band
algorithm.
Note that parameter X in modified three-band algorithm is usually predetermined using
accurate information for the inherent optical properties. However, such local information is
difficult to be accurately measured [44]. However, the X can be deemed as an “unknown”
variable and can be robustly regressed using non-linear recursive method during procedure
for determining the optimal relationship of [s−1 (λ1)-Xs−1(λ2)]s(λ3) versus [chla]. The results
(Fig. 6(b)) indicates that the modified three-band algorithm has the optimal accuracy in case
of [s−1(600)-0.2363s−1(580)]s(692), whose correlated coefficients are larger than 0.8302.
3.3.3 Variation three-band algorithm calibration
The backscattering by particulate matters were strongly related exponentially to the
wavelength at visible and NIR regions [15, 23], so the effects of bbp(λ) at s(λ2)a(λ2) and
s(λ3)a(λ3) can be semi-analytically minimized by s(λ1)a(λ1) using Eq. (15). To determine the
a(λ2) and a(λ3) using measurements of s(λ1), s(λ2) and s(λ3), four empirical spectral
relationships should be known: a(λ1) versus a(λ2), a(λ1) versus a(λ3), bbp(λ1) versus bbp(λ2),
and bbp(λ1) versus bbp(λ3), respectively. However, there is no field-measured backscattering
coefficient here, so the spectral relationship of s(λ1)a(λ1) versus s(λ2)a(λ2) and s(λ1)a(λ1)
versus s(λ3)a(λ3) were used to substitute the spectral relationships of backscattering for
initializing the variation three-band algorithm.
Fig. 7. Empirical spectral relationships. (a) a(600) vs. a(488) and a(610) vs. a(488); and (b)
s(600)a(600) vs. s(488)a(488) and s(610)a(610) vs. s(488)a(488).
By inputting the bio-optical data including s(λ) and a(λ), the variation three-band
algorithm is determined by non-linear iterative method while λ1, λ2, and λ3 are, respectively,
turning from 400 to 750nm, e.g., for each wavelengths combination of λ1, λ2, and λ3, the
constant coefficients of κ12, κ13, ζ12, ζ13, ξ12 and ξ13 as shown in Eq. (15)-(18) can be
determined using inputting data set of s(λ) and a(λ) at corresponding wavelengths. Then, the
π0, π1, and π2 as shown Eq. (19) can be determined by the non-linear iterative method
proposed by Chen and Quan [6]. The optimal variation three-band algorithm must be the one
with smallest RMS value. The results indicate that the optimal positions of three bands of
variation three-band algorithm are λ1 = 488, λ2 = 600, and λ3 = 610nm. Figure 7 shows the
empirical spectral relationships between the optimal bands of variation three-band model,
indicating that both a(λ) and bb(λ) at 600 and 610nm are strongly related to a(λ) and bb(λ) at
488nm, whose correlation coefficients are larger than 0.979. The variation three-band model
can be initialized as long as spectral relationships between selected bands are known. Figure 8
shows the optimal variation three-band algorithm regressed from calibration data set taken
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9036
from West Florida Shelf waters in 2000-2001, indicating that the relationships of a(610),
ap(610), and chla concentration versus variation three-band model were found to be
linear/non-linear and highly significant, whose correlation coefficients are larger than 0.9468.
Fig. 8. Optimal variation three-band model. (a) Total absorption estimation model; (b)
Absorption by chla estimation model; and (c) chla concentration, [chla], retrieval model.
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9037
Fig. 9. The relationship between RE and the chla concentration, where RE is the absolute value
of relative error.
The relationship between RE (relative error) and chla concentration was presented to
demonstrate the ability of these three algorithms in estimating chla (Fig. 9). We found that the
RE decreases with increasing chla concentration, but there is no statistically significant
relationship between them. Comparison of the variation three-band model to that of modified
three-band and three-band models indicates that the variation three-band model considerably
reduces the RE value and outperforms the three-band algorithm, especially at a low chla
concentration level (<1 mg m−3). These findings imply that the variation three-band algorithm
dose not require further optimization of spectral band positions and site-specific
parameterization to accurately estimate chla concentration in water bodies with widely
varying bio-optical characteristics taken in different seasons and years from West Florida
Shelf waters, even though the optimal spectral band positions and site-specific
parameterization are semi-analytically determined in the algorithm calibration procedures.
4. Discussion
As mentioned in previous, the samples with low chla concentration level (<0.2 mg m−3) were
deemed as outliers and ignored in model calibration and validation procedures due to the
weak absorption by chla. Figure 10(a) shows the lab-measured chla concentration plotted
against field measured absorption by chla at 610nm, indicating that the cha concentration is
weakly depended on ap(610) while the chla concentration is too low. Due to the low value of
ap(610) (<0.002 m−1), the noise of ap(610) was significant. That might be one of the reasons
for the poor performance of chla estimation in these low concentration levels. Figure 10(b)
shows the ratio of absorption by chla to total absorption (CTR) at 610nm, indicating that the
ap(610) is a very small part of a(610) for samples with low chla concentration, whose
CTR<0.7%. This is to say, 0.7% retrieval uncertainty in a(610) would result in 100%
uncertainty in ap(610) retrievals, which would lead to much larger uncertainty in remote
sensing of chla concentration. In fact, the uncertainty associated with a(610) retrievals is
1.24% derived by the variation three-band algorithm in this case study, so accurate estimation
of chla from remote sensing data is particularly challenging in coastal waters with low
concentration level. Additionally, recent intercomparisons have demonstrated that uncertainty
in remote sensing determined by approach of “radiometer measurements and data analysis
protocols” to be <5% under varied cloudy and sea state conditions [45], which would strongly
impact the accuracy of model construction results. In addition to this, there is ~5%
uncertainty contained in satellite-derived water-leaving reflectance when a very accurate
atmospheric correction method is used remove the atmospheric absorption and scattering
from satellite data even in Case I waters [42], which would further influence the retrieval
accuracy of a(610) from satellite images. These technical limitations make optical sensor
technology to be unable to derive chla concentration from coastal waters with low
concentration level (<0.2 mg m−3) Further work is required on this subject.
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9038
Fig. 10. Comparison between the lab measured chla concentration and field measured
absorption by chla in low chla concentration level (<0.2 mg m−3). (a) Lab measured chla
concentration versus field measured absorption by chla; and (b) CTR plotted against field
measured total absorption, where CTR denotes the ratio of absorption by chla to total
absorption.
Fig. 11. chla concentration plotted against ratio of water backscattering to total backscattering
at 670nm. Here, the total backscattering coefficients are derived using formula of
a(670)s(670).
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9039
According to results shown in Fig. 9, we found that the modified three-band algorithm
decreases 38.28% RMS values from the three-band algorithm, while the variation three-band
algorithm decreases 17.74% RMS values from the modified three-band algorithm. The former
improved performances may be attributed to the improvement in the negligible backscattering
scattering of suspended solids over NIR region in highly turbid waters, violation of
the“spectrally flat” assumption in optical complicated waters, and poor approximation of
coefficient γ, while the latter is related to the improvement in negligible absorption of
suspended solids at λ3 and negligible water molecular backscattering at red and NIR regions
(λ<800nm), except for highly turbid waters. By comparison, the former three limitations
more significantly impact the performances of chla concentration retrievals algorithm than
the latter two limitations in deriving chla concentration from turbid coastal waters.
Different limitations have different influence on chla concentration retrievals in different
water types. For example, the limitations of the “spectrally flat” assumption and the poor
approximation of coefficient γ may be violated in all water types because of the wavelengths-
depended of inherent optical properties of water quality constituents; the assumptions of
negligible absorption of suspended solids at NIR and negligible backscattering of suspended
solids over NIR region may work well in blue or clear waters, but may be violated in the
turbid waters with high suspended sediment concentration; on the contrary, the assumption of
negligible water molecular backscattering at red and NIR regions may work well in turbid
waters, but may be violated in blue or clear waters.
During past several years, many semi-analytical algorithms have been successfully used
for estimating chla concentration from coastal and inland waters. For example, Li et al. [46]
suggested a semi-analytical algorithm combining both three band indices and a baseline
algorithm to estimate chla concentration, and then tested the algorithm in three eutrophic and
turbid reservoirs. Their algorithm accurately estimated chla concentration with 31.4% RMS
for water samples with the chla concentration range from 1.4 to 146.1mg/m3. An improved
OC4v4 algorithm was suggested by Chen and Quan [6] for estimating chla concentration
from Yellow River Estuary. That improved OC4v4 algorithm produces ~32.7% RMS in
estimating chla concentration in that regions. Moses et al. [47] constructed a three-band
algorithm for retrieving chla concentration from Azov Sea and Taganrog Bay, Russia, and
determined that the three-band algorithm gave consistently highly accurate estimates of chla
concentration, with 21.05%. In comparing the performance of variation three-band algorithm
in this study with the optimal results of these algorithms, they found that the RMS of chla
concentration prediction was comparable: 24% RMS in variation three-band algorithm versus
21-33% RMS in these semi-analytical algorithms.
The variation three-band algorithm presented in this study may be applied to NASA
HYPERION and the Compact High Resolution Imaging Spectrometer (CHRIS). As an
example, the HYPERION channels at 487.87, 599.90 and 609.97nm is quite close to the
position of λ1 = 488, λ2 = 600nm, and λ3 = 610nm, respectively. These findings indicate that,
provided that an atmospheric correction scheme for visible and near-infrared bands is
available, the variation three-band algorithm may be used for the quantitative monitoring of
chlorophyll-a concentration from the HYPERION sensor in West Florida Shelf waters.
However, the field data set used in this study is quite small and covers only a narrow range of
natural waters, so it is insufficient to completely validate the accuracy of the variation three-
band algorithm. More independent tests with field-measured data are required for the
validation and improvement of variation three-band algorithm. The variation three-band
algorithm should be used for estimating chla in coastal waters, even though it is essential to
reinitialize the model statistic parameters using local aquatic bio-optical information. We
suggest calibration and validation of the algorithms based on more in situ measurements of
waters with different optical properties.
There are some caveats that need to be considered when attempting to apply the variation
three-band model to satellite. Since the variation three-band algorithm relies strongly on
reflectance in the red region, there are specific hurdles that need to be addressed. The strong
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9040
absorption by water in red band greatly reduces the magnitude of the recorded signal in the
red region, reducing the signal-to-noise ratio and enhancing the effect of the inherent noise in
the recorded signal [8], This may make the variation three-band algorithm sensitive to
inherent noise in the measured signal. The parameters related to spectral slope in Eq. (17)-
(19) may vary based on the nature of waters under study. For example, Bowers et al. [15]
indicated that the specific scattering coefficient depends on the nature of the scattering
particles: their size, shape, refractive index and density. Suzuki et al. [48] determined that the
differences in phytoplankton pigment composition of each water mass may lead to differences
in phytoplankton absorption spectral of each water mass. Therefore, local information or an
improved algorithm is needed to reinitialize the parameters of variation three-band algorithm
in practical remote sensing application, when the bio-optical properties are different from
these used for model development in this study.
5. Summary
In this study, three semi-analytical models for remote sensing of chla concentration were
constructed by specifying the optimal bands. The three-band model has the optimal
wavelengths of λ1 = 620nm, λ2 = 712nm and λ3 = 730nm; the modified three-band algorithm
has optimal accuracy when [s−1(600)-0.2363s−1(580)]s(692); and the specification of the
wavelength in the variation three-band model is λ1 = 488, λ2 = 600nm, and λ3 = 610nm.
Evaluated using two independently collected data sets in West Florida Shelf, USA, all modes
are found to have an acceptable accuracy in estimating chla concentration, expect for three-
band model. Comparison of the variation three-band model to the other two semi-analytical
models indicates that the variation three-band model considerably reduces the uncertainty and
outperforms the three-band and modified three-band algorithms, especially at a low chla
concentration levels (<1 mg m−3). Using the variation three-band algorithm to retrieve chla
concentration in the West Florida Shelf waters decreased uncertainty by 18% compared to the
modified three-band algorithm and 56% uncertainty from the three-band algorithm. The
significantly reduced uncertainty in chla estimations is due to removal of absorption effect of
gelbstoff and suspended solids and the effects of water backscattering. The spectral position
of variation three-band model is quite closed to the position of HYPERION sensor. These
findings indicate that, provided that an atmospheric correction scheme for visible and near-
infrared bands is available, the variation three-band algorithm can be used for the quantitative
monitoring of chlorophyll-a concentration from the HYPERION sensor in eutrophic coastal
waters such as West Florida Shelf waters, even though it may be essential to reposition the
wavelength of the three bands for the given study area accordingly or reinitialize the
parameters of variation three-band algorithm using local bio-optical information.
Appendix I
In order to determine the optimal bands for three-band algorithm, the band turning method is
used by Gitelson et al. [8]. To find the optimal position of λ2, initial position for λ1 was set to
λ1,A and the initial position for λ3 was set to λ3,A. As λ2 was turned from λ2,I to λ2,T, the RMS2
of [R−1rs(λ1,A)-R−1rs(λ2)] Rrs(λ3,A) versus [chla] was calculated. RMS2 varies with wavelength
λ2, its minimum occurs at λ2,B. Therefore, λ2,B was selected for λ2. To determine the optimal
position of λ1, λ2=λ2,B and λ3=λ3,A was used, and λ1 turned from λ1,I to λ1,T. the RMS2 of
[R−1rs(λ1)-R−1rs(λ2,B)] Rrs(λ3,A) versus [chla] was again computed for each λ1. Results indicates
that minimum RMSE exits at λ1,C. Thus, λ1,C was selected for λ1. The optimal λ3 was
determined by setting λ1=λ1,C and λ2=λ2,B, and the minimum RMSE occurs at λ3,D. Therefore,
the optimal three-band algorithm calibrated by bands turning method suggested by Gitelson et
al. [8] is [R−1rs(λ1,C)-R−1rs(λ2,B)] Rrs(λ3,D). Figure 12 shows the flowchart of band turning
method for searching optimal band of three-band algorithm, indicating that the band turning
method can be denoted to: (1) given an initial point A, calculates RMS2 at each point along
line L2, and finds point B has minimum RMS2; (2) reinitializing the band conditions using
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9041
point B, and computes RMS2 for very point in L1, and finds point C has minimum RMS2;
and (3) reinitializing the band conditions using point C, and computes RMS2 for very point in
L3, and finds point D has minimum RMS2. Thus, point D is the optimal results of band
turning method. In fact, the bands turning method uses the minimum RMS2 of three lines to
approximate to the minimum RMS2 of the whole bands searching ranges. Thus, the optimal
bands determined using bands turning method are the local optimal bands but not the global
optimal bands.
Fig. 12. Flowchart of band turning method for searching optimal band of three-band algorithm
Acknowledgments
This study is supported by the Science Foundation for 100 Excellent Youth Geological
Scholars of China Geological Survey, open fund of Key Laboratory of Marine Hydrocarbon
Resources and Environmental Geology (MRE201109), Serial Maps of Geology and
Geophysics on China Seas and Land on the Scale of 1:1000000 (200311000001), and the
National Natural Science Foundation of China (No. 41106154). We would like to just express
our gratitude to Prof. K. L. Carder (download from SeaBASS) for providing the bio-optical
data set for this study. We would also like to express our gratitude to two anonymous
reviewers for their useful comments and suggestions.
#186891 - $15.00 USD Received 12 Mar 2013; revised 16 Mar 2013; accepted 18 Mar 2013; published 4 Apr 2013
(C) 2013 OSA 8 April 2013 | Vol. 21, No. 7 | DOI:10.1364/OE.21.009024 | OPTICS EXPRESS 9042