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Examination of the Laboratory Department / Ministry of Health
private Laboratory License
Environmint (April 2019) For the
3o
Choose the most tikely correct answer (Bacteriotogt Portlon)
from acute pharyngitis:
Q-1- Eleven years old boy complaining
a- The most likely causative pathogen is viral
b- Corynebacterium could be the etiology
c- The swab-taken from the area is of no value
1rE Gram,posi olysis is of imgortance in this situation
Q-2- twenfo two lon:
a- The most the urinary Passages
b- Anaerobic etiology could be of value
c- The recurrence of uTIs is of low occurence in this age
d- Advice the patient for area cleanliness is of no importance
got foreann impetigo:
Q-3- Seventeen yeuts old young male
a- Usually this type of lesion is not contagious
b- Mostly it is caused by Streptococcus species especially alpha hemolytic type
c- Usually the anaerobes contributed in this are
'..4--.1 StreP
Thirty dry cough with headache:
e_4_
a- Must oratory investigations
G) Gram negative coccobacilli could be of importance
c- Compli.it.d Staphylococcal respiratory infection might be considered
d- Streptococcal contribution must be in' otved in this case
dischatge with vulva itching:
e-5- ForU years old female garn recurrent vaginal syphilis
a- Treponemal etiology riust be considered as her partner diagnosed to be a
patient
b- Gonococcal etiology is far away from involvement
(si The pronounced cause here is to be chlamydia
-*al Cand_idiasis is less cofitmonly to occur in this age group
game leg injury:
Q-6- Thity three years old !1ale got football
a- The area debridement is always not advisable here
b- There no riskiness in this case from anaerobic pathogens
c- The infection that occ\r secondary to the injury could be a mixed one
d- The usage of disinfeptant is always contraindicated
fold infection with swelling and
e-7- Housewiie aged fifty years old gain big toe nail
discomfort;
a- Mostly the laboratory must not seek for blood sugar measurement
b- The etiology might be of mixed in nature
 c- It is not advisable to avoid house hold bleach in this situation
d- Maleoa[e more prone to gain this type of infection
t m .Ul 1
than females
r- Twenti
Q-q- I wenty seven years old flbmale complaining from
Seven y
continuous eye redness with
purulent discharge;
a- It is advised in this case to leave both eyes away from
cleanliness
b- It is advised always to use antimicrobial agenteye drops for long dgration to get

ember of gram positive cocci

in suspicion
I cavity dsntal caries:

ffeptococcal microbe
involved in this situation occurrence
must be think regarding this case
fluid brought to your laboratory:
st be at 500 {pm
pathogens in this c€Ne
c- Nonnally this fluid brought to your taboratJry
with a little a:nount of RBcs
g centrifugation is not indicated always here
^"
Q- I l - In Clostridium perferingenes

a- Its a toxin (lecithinase) could not reco gnrzedthrough

Nagler,s test
b- The gangrene require slowly interventlon
debridement . Lr
supplied- with wound extensive

c- The spores plus microorganisms vegetative

forms are found in soil plus human
microbial flora
d- Its gas gangrene is mostly prevalent
even in rur spoiled wounds

Q- l2- Belonging Clostridium dfficile

positive anaerobic bacilli, non spore forming,
motile
age mostly mediated through enterotoxin (toxin
A) plus
c- The prevention of cross infections among
nosocomial measurements has no
relation to hygiene
d- The sequel of pseudomembranous colitis
(antibiotics associated colitis) of
minimum mortarity in some sifuations
 Q- 1 3- Regarding buccal microbiology

/ into tissues
(=
t),B"r:ut microbial flora have harmfrrl efforts as they try to access
causing micro abscess
b- Candidal species and Actinomyces have no role in buccal pathology
c- Streptococcus salivarius, Streptococcus mutans, and Streptococcus sanguis are
rarely found in the early born nevr babies
d- The buccal cavity of the new bom are usually non sterile due to amniotic fluid
swallowlng

Q- 14- Belongng Chlamydia

a- Mostly garn RNA genome not DNA genome

b- They are sensitive to many antibacterial agents like ethanol or low
concentration of formaldehYdes
c- Its grow very well in cell free media
d- It has no cell wall just like Rickettsiae

Q- 15 - In Mycoplasma infections

a- It is the largest gram negative pleomorphic microorganism which is free

living
b- It is mostly non motile
c- The suspected throat or respiratory secretions usually inoculated in to
media that composed of low concenffations from glucose and phenol
d- Their gfowing colonies will not adsorb any erythrocytes towards them

Q- 16- Regarding Brucella

a- It is a zoonotic infection could fiansmitted from animal source and vise versa
b- It is strict aerobic microorganisms and its optimum pH equal to 6.6-7 .4
c- Its cultivation media has no need for additive requirements
d- For its growth; the media require at least 4 weeks in order that the visible
colonies reach 0.1-1 pm in diameter

Q- 1 7- Regarding C Io stridium tetanospasmin

@Gets inactivated at 66 "C for 5 minutes

b-Can get its toxoid with high concenfiation of formaldehydes
c-It considered as a defective antigen
d-lts main action through increase neuromuscular signals transmission
 hemolysin (Streptolysin)
Q- 18- Regarding sffeptococcus

(t-6-tt
ar6t eptolysin o demonstrable in deep colonies as
it is oxygen labile
is not a strong antigenic
c-It is not heat labile
d-lt is not influenced by cholesterol
isolated from a urine sPecimen (10t
icillin and gentamicin has been of no
action is to be:

a- Do not applied further workup processing - _^r.r^a* na i,.ranri

b- Suggest to the laboratory tnut little .olony count may reflect an infection
of
cF Determine if florescent microscopy is avaitable for the diagnosis
actinomYeosis . '
d- Consider vancomycin as an alternative drug
Q-20 - Regarding the cerebrospinal fluidtoanalysis:
any tube of CSF brought to the
a- Always apply your invesiigations
Iaboratories
b- Try to cenffifuge the specimen_ at 3000 rpm for 20 minutes is of no vatue
etiologies
o- The kit of latex ag-gtutination for the CSF infection
normally f6unded at low levels in the tube of the
specimen
@ffis
infections:
Q-21- Regarding sinus microbial
the list of their etiology
a- The anaerobic bacteria must be excluded from
b-Theroutoforganismsishematogenou]Sonly
bony cavities is rare
c_ The rout of organisms snfiry from neighboring
d- The occurrettti is usually in summer season only

Q-22-InStreptococcalimpetigoinfection: other persorurel

a- The lesion is usually noi contagious to
b-The lesion in mostly crusty with glistaring
farmers
c-The infection occ'r as deal with soii in the
d-Gram film from the lesion area is of no importanoe
three species that causing brucellosis is best
e-23- Brucella abortus, one of the
described by one of the following statements:
tract and is an occasional pathogen
a- commonly inhabits the canine respiratory
for humarrs, strongly urease positive
 b- Grows both in carbon dioxide environment and under anaerobic
conditions
(g) Typically infects cattle, requires 5- I 0o/o carbon dioxide for growth, and is
-rnhibited bv the dye thionine
d- Typicatly is found in infected animal bites in humans and can cause
hemorrh agrc septicemia in animals

Q-24- Which of the following is the predominant flora of the mouth that is the
major cause of dental caries:

a- u. - hemolytic Streptococci
b- Lactobacillus
c- Staphylococcus epidermidis
d- Bacteroids fragilts
Q-25- Escherichia coli O 15 7lH7 is a newsworthy microorganism that has been'
isolated from foods with increasing frequency. Its characteristic biology is best
described by which of the following statements

a- It secretes erythrogenic toxin that causes the characteristic signs of scarlet

fever
b- It produces toxin that blocks protein synthesis in an infected cell and
carries a lytic bacteriophage that produces the genetic inforrration for toxin
production
c- It produces at least one protein toxin consisting of two subunits, A and B ,
that cause severe spasmodic sough usually in children
@ It secretes exotoxin that has been called verotoxin and shiga like toxin
infection is mediated by specific attachment to mucosal membranes

Choose the most lihely correct answer (Parasitologt Portion)

zoonotic: f
Q-26- One condition is not
a-Swimmer's itch
b-Scabies
c-Visceral larva migrans
GC.,taneous larvamigrans
Q-27 - Stained blood smear reveal giant platelets with retisulocytes that sontain
Schuffrrer's dots and ring forms and the Schizonts composed from 12-24
merozoites; this run with;
falcipantm
vivax
 under anaerobic
b- Grows both in carbon dioxide envirsnment and
conditions
for growth' and is
G; Typically infects cattle, requires 5- l}oAcarbon dioxide
'YhiUited
by the dYe thionr e
and can cause
d_ Typicalty is ro'nd in infected animal bites in humans
hemorrhigic septicemia in animals
is the
is the predominant flora of the mouth that
e-z4-Which of the following
major cause of dental caries:

a- o - hemotYtic StrePtococci
b- Lactobacillus
a- StaPhYlococcus ePidermidis
d- Bacterotds fragili

o
coli microorganism that has b6en
Q-25 - Escherichia
characteristic biology is best
isolated from foods with
described by which of the fll g statements
\

a- lt secretes erythrogen+ that causes the characteristic signs of scarlet

fever
b- It produ..q toxin th ockspioduc.r
protein synthesis il.TTI^t-|1:ril-:i
th. genetic inforrration for toxin
,r** iiil e ftat
\^
production \ -^.^^:^t)--^r+.,,^ ..,,r.,rnifc A
A and
an, B
::""?or.o"res at one pl,otein toxin consisting of two subunits, '
that cause severe
/YVrv ur sp dic cough usually in children
ad) It secretes exotoxin that has been called v rtoxin and shiga like toxin
'--7
infection is mediated by specific attachment to cosal membranes

most tihety correct onswer (Parasitologt

Portion)
Choose the

Q-26- One condition is not

zoonotic: '
a-Swimmer's itch
b-scabies
c-Visceral larva migrans
Gcutaneous larvamigrans . .-r-t^r^+^ --,!+L-o*ir.rrr^nrrreq thct c.ont:
reveal giant plateletg with reticulocytes that
sontam
e-21- Stained blood smear
Schuffirer,s dots and ring forms and Schizonts composed from 12-24
itt.
merozoites; this run with;
arum
c-Plasmodium ovale
d-Plasmodium malariae

Q-28- The diagnostic criteria for Entamoeba histolytica is:

\.L*ge karyosome with faint visible nuclear membrane
Qlentral located karyosome
c-large glycogen vacuoles
d-gain flqgella to one pole
Q-21-516ich parasite not need intermediate host?
a-Schistosomo mansoni
b-,Scft rs to s oma he amat o bium
c-Giardia lamblia
d-Taenia solium
q30- White blood cells could be used as positive ooptrol for procedrnes except:
aCiemsa stain for Trypanosomes
blron hematoxylin stain '
c-WC mount
d-Trichrome stain

Arcose the most lihely correct answer (Mycologt Portion)

Q-31- Cocsidioidomycosis is called as:

a. Death fever
b. Valley fever
@ Chache fever
d. Black fever

Q-32- Tinea nigra is fieated through:

a, Hydrochloric acid
b. Sulphuric acid
c. Salicylic acid
d. Alcohol

Q-33- Rhizopzs is a causative agent of

a. Mucormycosis
b. Candidiasis
c. Aspergillosis
d. Histoplasmosis
 Q-34- lmmune response to fimgal infections is the formation of:

a. Granulomas
b. Grmules
c. Spores
d. Conidia
Q-35- Drug that disrupt mitotic spindle by binding to tubulin, and liver toxicity is its
side 6ffect is knovrm as:

a.
b.
c.
d.
ts

Choose the most lihely correct answer (Yirologt Portion)

Q-36- What isthe most coilrmon cause of aseptic meningitis of viral etiology:
.-\,
(g t.ntero vrn$
b. Herpes virus
c. Arbo virus
d. Retrovirus

Q-37- Finding of large multinucleated clump of cells in bronchial secretions of 2 yw:

old girl with acute bronchopneumonia suggest that this infection due to:
a. Bordetella pertussis
b. EBtl
c. Rhinovirus
d. RSV

Q-38- All of the following Picornaviruses are resistant to acidity of stomach

except:
a. Coxackievirus A
b. Coxackievirus B
c. Echo vints
d. Rhino vinn

Q-39- What type of viruses containing enzyme lysosomes to aid in their

infection?
@nacteriophage
n. Animal viruses
c. Fungal vinrses
 d- Humm vintses
Q4C Viroi& se tmusml in that theY:
@ have no capid Protein or enveloPe
b. ue smtain RNA
c. are rmely visible with light microscope
d. cm cause disease in Plant
e4l - Creitzfeld Jakob disease, Kuru, scrapie and mad sow disease are
caused by:
aliroids
b. Retrovirus
c. DNA viruses
d. Prions

Q-42- Polio viruses can be tyPed bY:

a. single radial hemagglutinin
b. Hemagglutinin inhibition
c. DEAFF test (used for isolation of CMV)
d. Hybridization with nucleis acid probe
Q.43- Stmdtrd PcR consist of all the following except:
@Oenaturation" annelling md ligafion steps
b. dNTPs
c. Mg ++
d. Taq polymerase
Q-44- Regarding Rubella virus, which one is the most accurate:
a. Systrimic infection with rubella is often causes severe liver
damage result in cirrhosis
b. The main soruoe of vinrs is adult recovere from disease but are
chronic carriers of virus
c. If a pregnant woman is infected dtrring l't trimester, significant fetal
abnormalities typically result
d- Immunization of both male and female health care worker with fonnalin
inactivated vaccine is recommended
Q-45- Regarding RsZ,which one of the following is most accurate.
a. RSY oauses tumor in newborn animal not in human
b. R^Stzvaccine is recommended for all children before enfiry to
school
c. RSV is important cause of bronchiolitis in infants
d. R^yZ form inffanuslear inclusion bodies within neutrophils
 d. Humm vrruses
/J Q40- Viroids tre unusual in that they:
@ have no capsid protein or envelope
b. are contain RNA
c. are rmely visible with light microscope
d. can cause disease in Plant
e41 - Creitzfeld Jakob disease, Krlru, scrapie and mad cow disease are
caused by:
a. Viroids
b. Retrovirus
c. DNA viruses
d. Prions

Q-42- Polio viruses can be tYPed bY:

a. single radial hemagglutinin ';

b. Hemagglutinin inhibition
c. DEAFF test (used for isolation of CMV)
d. Hybndization with nucleic acid probe
Q43- Standard PCR consist of all the following except:
@denaturation, annetting md ligation steps
b. dNTPs
c. Mg ++
d. Taq polymerase
Q-44- Regarding Rubella virus, which one is the most accurate:
a. Systemrc infection with rubella is often causes severe liver
damage result in cirrhosis
U. fne main source of virus is adult recoversd from disease but are
chronic carriers of virus
c. If a pregnant woman is infected during 1" trimester, significant fetal
abnormalities typically result
d- Immunization of both male and female health care worker with formalin
inactivated vaccine is recommended
Q-45- Regarding RSV, which one of the following is most acctnate:
a. RSV causes tumor in newborn animal not in human
b. RsZvaccine is recommended for all children before entry to
school
c. is important cause of bronchiolitis in infants
RSV
d. RSt'form intranuclear inclusion bodies within neutrophils
 \
\

Q+e- A *wa b hfrtug in isolated area, when slcunk appeared and bite her
Iegs md itnrr Uoto@l, the first thing to do is:
a- Rrswuehcr thC rabies isn't problem because skunks do not carr rabies.
b Giw k veine and hlper immune globulin immediately
c- Moilitm the animal for l0 days and only treat her if sign of rabies appear in animal
d- Testthe patient serum for Ab now and after l0 days to see if there is increasingtiter
Q47- All the following viruses are ds-DNA except:
a. Polyoma virus
b. Hepadnavints
c. Herpesvfiases
@Partoviruses
Q-48- Regarding rotaviruses, which one is most accurate:
a. Rotavirus is a major cause of nosocomial diarrheainlcu
@vaccine against rotavirus contain live attenuated virus as *
unmunogen
c. rotavirus is nonsegmented ss-RNA genome & it has no
polymerase enzyme
d. diagnosis of rotavirus diarrhea is typically made by detection of 4 fold or the
greater rise in Ab titer
Q-49- Regmding viral replication, which one is most appropriate:
a. Cyopathic effect typically occurs during the eclipse phase
b. the early protein are typically enzymq while late protein are
capsid protein
c. the assembty of non enveloped virus typically occurs as virion
buds from the cell membrane
d. In/luenza virus synthesize their mRNA using host cells encoding RI.IA dependent
RNA polymerase
Q-50- Which one of the following viruses causes perinatal infection?
a. EBV
b. JC virus
c. Ir{orovirus
d. CMV
 \
\

Q+0- A nwa fu h+4 ix isotated area, whe,n slcunk appeared

Iegs md 6f.' Ep to boryttnt, the first thing
and bite her
to do is:
a- Reecqrueher th* rabia isn't problem
because slcunks do not carryrabies.
b Gi'rrc k vrccine and hyper i*t"i11e globulin immediately
c- Monitm the animal for days and ooly treat G ir sign of rabies
-10
d- Test the patimt seium for appear in animal
Ab now and irto l0 days tJsee if there i, io,,.asing
Q+z- All the following viruses are ds-DNA except. tite
__ a. Polyoma virus
b. Hepadnavints
c. Herpesvinrses
@Po-oviruses
Q-48- Regarding rotaviruses, which one is most accurate:
a' Rotavirus is. major cause of nosocomial
diarrhe a inICU
@vaccine against totur.i*s contain live affehuated
.,-
unmunogen
virus €,,
c. rotavirus is nonsegmented ss-RNA
genome & it has no
polymerase enzyme
d' diagnosis of rotavirus diarrhea is typically
made by detection of 4fold or the
greater rise in Ab titer
Q-49- Regmding viral replication, which one is most
a' Cytopathic effect typically occurs
appropri :
during the eclipse phase
b' the early protein are typi.aty enzyme,
*hile late protein are
capsid protein
c' the assembly of non enveloped virus
typically occurs as virion
buds from the cell membrane
svnthesize ther mRNA using host ce[s
encodins RNAdependent
"^K[:;;i";T:
Q-50- which one of the following viruses causes perinatal
infection?
a. EBV
b. JC virus
c. Norovirus
d. cMr/