Professional Documents
Culture Documents
From 34 To 45
From 34 To 45
Foal died with pneumonia 24 weeks, lung sprcimen turned to bagteriology lab, .1
the isolated colony was macroscopically, small-medium mucaid, round colonies; no
hemolysis and note characieristic salmon pink color, gm stain +ve plodmorphic rods,
....... the isolate is
C. Equi (Rhococcus equi) →CAMP (+VE Nitrate (+ve), Catalase (+ve), sugar >
fermentation (-ve)
Pus from a chronic infected Sne leg wa urned te bacteriology laboratory .2
Enumerate name of susprcted bacterial isolates .1
Covis - Actinobacillus (pseudononts Mallei- Histoplasma farciminosum-
Sporothrixschenkii(dimorpie fuogi)
Confirmatory laboratory dirterentlation between them .2
Disease in Horse Cause Morphology Strauss Mallein test
reaction
ZN +ve ZN +ve
Acid and Alcohol fast Acid fast but NOT alcohol fast
Diagnosis of T.B
A. Field diagnosis →
Allergic tests (Tuburclin test) for -> MTB and M. bovis: it (depend on cellular
immunity) (T. cells) that develop 30-50 days post infection so these tests can be
done 30-50 days post infection. (delayed hypersensitivity)
Tuberculin test (accuracy, true positive up to 65%)
it is standard antimortem diagnostic test??? Once it give tve →slaughter of the
animal types →1. old tuberculin
2, PPD****** purified protein derivatives" better than old test & more accurate
PPD →MTB, M.bovis, M.avium →inoculate in glycerol broth →incubate(37C,7week)
→centrifugation →supernatant + trichloroacetic acid "precipitate protein "
→purification →0.1 mi. ID.(skin tail fold)
result : after 72 hrs.
1. Same skin thickness →-ve.
2. skin thickness →4mm →+ve "granuloma formation→+ve →slaughter.
B. Laboratory diagnosis →
1. Samples →
a. Sputum (pulmonary infection), urine, peritoneal or pleural aspirate-> MTB, human.
b. Lymph node (after slaughter), pus, milk, trachea-bronchlal aspirate, feces, semen
→M. Bovis, cattle.
c. Feces - rectal swab -) M.avium - paratuberculosis
2. Samples processing (precautlons of samples handling) →
a. Aim of sample processing →(1. Liquefaction of samples (Sputum or L.N),
2. Concentration of M.O
3. decontamination of samples)
b. Steps →
- + + Guinea pig
- + - Rabbit
- + - Cattle
+ - - Fowl
- + + Man
Actinomyces Nocardia
Anaerobic, long filamentous baccilli Aerobic
ZN -VE (blue bacili & blue ZN +VE (pink bacilli & blue
background) background)
-VE On SDA: +VE
Normal flora On Soil
+ Spore: -ve
- Chronic granulomatous mastitis &
1. Actinomyces bovis = lumpy jaw (Gr Abortion in cattle Cattle farcy
+ve) -> chronic Pyogranulomatus (lymphangitis) Tuberculus like disease
disease affect human and all animals) (ulcerative lesion)
CHAR. BY SULPHER GRANULES in
the pus
2- Actinemyces pyogenes: summer
mastitis
Actinobacillosis Actinomycosis
Actinobacillus ligneresi Actynomyces bovis
Woody tongue in cattle Lumpy jaw in cattle
Gr -ve Gr +ve
Not stained by ZN ZN -VE
Aerobic Anaerobic
No sulpher granules CHAR. BY SULPHER GRANULES
Bacillus Anthracoids
• Nagler's reaction*** (On lactose egg yolk milk agar medium) →B.Cereus and
B.mycoides give +ve result that characterized as pearly opalescence zone
surround the colony due to lecithin hydrolysis, Non lactose fermenter
B.anthracis: weak lecithenase activity-
Gelatin media: fir tree absent (rapid liquifaction)
• Pathogenicity →Non pathogenic except →B.cereus →food poisoning in man,
B.subtilis →severe eye infection
• N.B →
Sudden death causing M.O b. anthracis (no hemolysis on bl.agar), cl. chaouvii, .1
pasterella hemolytica (no hemolysis on bl.agar)
B. anthracis B. anthracoid
End of bacilli Square Rounded
Spore Central Central / terminal /
subterminal
Non bulged Bulged / non bulged