CHAPTER 12: RAPID PLASMA REAGIN (RPR) CARBON AGGLUTINATION TEST 38

Review Questions
and Results Sheet

Specific Learning Objectives:

• Identify the causative agent of syphilis.
• Describe the mode of transmission for syphilis.
• Differentiate treponemal test to non-treponemal test for syphilis.
• Discuss the principle of flocculation.
• Perform rapid plasma reagin carbon agglutination test for syphilis.

DIRECTION: Draw the observed reaction in the box below:

Positive Control Test Sample Negative Control

• Write if the test sample is reactive, weakly reactive or non-reactive on the space provided
below.

TEST RESULT:

Immunology and Serology Laboratory Exercises

 CHAPTER 12: RAPID PLASMA REAGIN (RPR) CARBON AGGLUTINATION TEST 39

QUESTIONS:
1. What is the causative agent of syphilis?

2. Briefly describe the mode of transmission of the syphilis agent.

3. What is the difference of treponemal test for syphilis to non-treponemal test for syphilis?

4. Briefly discuss the principle of flocculation.

Immunology and Serology Laboratory Exercises

 CHAPTER 13: Anti-Hepatitis B surface Antigen Test (Anti-HBsAg Test) 44

Review Questions
and Results Sheet

Specific Learning Objectives:

• Identify the causative agent of Hepatitis B.
• Describe the principle employed in the detection of Anti-HbsAg.
• Determine the importance of HBsAg in the diagnosis of Hepatitis B infection.
• Perform anti-HbsAg test.

RESULTS: Upon testing, draw your observation in the box provided below.

What is your interpretation of the result shown above?

Immunology and Serology Laboratory Exercises

 CHAPTER 14: Human Immunodeficiency Virus (HIV) Testing 50

Review Questions
and Results Sheet

Specific Learning Objectives:

• Identify the causative agent of AIDS.
• Enumerate the different routes of transmission of HIV.
• Describe the pathogenicity of HIV.
• Perform rapid chromatographic assay for HIV.

RESULTS: Upon testing, draw your observation in the box provided below.

Interpret the results shown above.

Immunology and Serology Laboratory Exercises

 CHAPTER 14: Human Immunodeficiency Virus (HIV) Testing 51

QUESTIONS:
1. What is the causative agent of AIDS?

2. Why type of cell does the AIDS virus infects? Explain the importance of this cell, and
briefly describe its importance in the immune system.

Immunology and Serology Laboratory Exercises

 CHAPTER 15: Anti-Streptolysin O (ASO) Test 57

Review Questions
and Results Sheet

Specific Learning Objectives:

• Describe the importance of ASO in determining streptococcal infections.
• Enumerate the toxins produced by Group A beta-hemolytic streptococci.
• Perform Anti-streptolysin O latex particle agglutination test.

QUALITATIVE RESULT: Draw the reaction observed in the box provided below.

QUANTITATIVE RESULT:

Immunology and Serology Laboratory Exercises

 CHAPTER 15: Anti-Streptolysin O (ASO) Test 58

QUESTIONS:
1. What is the significance of testing ASO in determining streptococcal infections?

2. List some of the toxins produced by Group A beta-hemolytic streptococci.

Immunology and Serology Laboratory Exercises

 CHAPTER 20: Rabbit Immunization Experiment 64

Results Sheet

Specific Learning Objective:

• Perform rapid slide test of serum for detection of rheumatoid factor.

RESULTS: Upon testing, draw your observation in the box provided below.

Immunology and Serology Laboratory Exercises