Test no :2.

3 ACIDITY TEST
Aim : To determine Acidity ( base capacity) of the given sample.

Principle :

The mineral acids present in the sample which are contributing mineral acidity can be

Calculated by titrating of neutralizing samples with strong base NaOH to P H 4.3. The CO2

And bicarbonates ( carbonic acid ) present and contribute CO2 acidity I the sample can be

Neutralized completely by continuing the titration to PH 8.2.

Apparatus:

1. Burette.
2. conical Flask.
3. Pipettes.

Reagents:

1. Standard sodium hydroxide (0.02N).

2. Phenolphthalein indicator.
3. Methyl orange indicator.
4. Sodium thiosulphate.
5. Carbon dioxide free distilled water.

Procedures:

1. Take 100ml of the given sample in an Erlenmeyer flask.

2. Add 1 drop of 0.1N sodium thiosulphate solution to remove the residual chlorine if present.
3. Add 2 drops of Methyl Orange . The sample turns pink.
4. Proceed with titration until the colour changes to yellow.
5. Note down the volume of the NaOH added ( V1).
6. Take another conical flask containing 100ml of water sample . Add 2 or 3 drops of
Phenolphthalein .
7. Proceed with titration until the sample turns pink .
8. Note down the total volume of NaOH added ( V2).

Calculations:

 Mineral acidity due to mineral acids (as CaCO2) (mg/l).

= V1 X 1000

ml of sample taken

 CO2 acidity due to CO2 ( as CACO2) (mg/l)

= V2 X 1000

ml of sample taken
 Observation and result:

Methyl orange indicator Phenolphthalein idicator

Sample Volume Initial Final V1 NaOH Initial Final V2 NaOH used (ml)
details of Burette burette used Burette burette
sample reading reading (ml) reading reading
(ml) (ml) (ml) (ml) (ml)
Sample 1 100 0 5.5 5.5 5.5 5.5 12 6.5 6.5 colourless
pink to To pink
yellow
Sample 2 50 12 19.5 7.5 7.5 19.5 25 5.5 5.5
pink to
yellow
Average V1 6.5 Average v2 6

1st sample (V2-V1) x1000

100
=6.5/100x1000=65mg/l

2nd Sample = (V2-V1) x1000

50
=6/50x1000=120mg/l

RESULT: The acidity of sample is 120mg/l

The BIS Standard for acidity is =