This report is generated by GainData® (arigo's ELISA Calculator). https://www.arigobio.

com/ELISA-calculator

Experiment name
BCA 15.6.2023

Standard concentration unit

ug/ml

Equation Linear ID Well(s) Raw data Average SD (OD) Dilution fa

11 Daudi neg B3, B4 0.1, 0.126 0.113 0.013 1
a 0.000956 Daudi pos C3, C4 0.091, 0.112 0.102 0.011 1
b 0.088271 Raji neg D3, D4 0.124, 0.179 0.151 0.027 1
Raji pos E3, E4 0.106, 0.124 0.115 0.009 1

R^2 0.994 12 Daudi neg F3, F4 0.148, 0.124 0.136 0.012 1

Daudi pos G3, G4 0.187, 0.208 0.198 0.01 1
Raji neg H3, H4 0.117, 0.134 0.126 0.008 1
Raji pos A5, A6 0.161, 0.166 0.164 0.003 1
HCT29 neg B5, B6 0.163, 0.169 0.166 0.003 1
HCT-29 pos C5, C6 0.119, 0.123 0.121 0.002 1

13 Daudi neg D5, D6 0.095, 0.112 0.104 0.008 1

Daudi pos E5, E6 0.097, 0.103 0.1 0.003 1
Raji neg F5, F6 0.138, 0.151 0.145 0.006 1
Raji pos G5, G6 0.144, 0.171 0.158 0.014 1

BL A3, A4 -0.003, 0.002 -0.001 0.002

S1 A1, A2 1.962, 1.901 1.931 0.03
S2 B1, B2 1.587, 1.516 1.551 0.035
S3 C1, C2 1.095, 1.089 1.092 0.003
S4 D1, D2 0.842, 0.828 0.835 0.007
S5 E1, E2 0.615, 0.619 0.617 0.002
S6 F1, F2 0.355, 0.342 0.349 0.006
S7 G1, G2 0.182, 0.166 0.174 0.008
S8 H1, H2 0.037, 0.035 0.036 0.001
ISA-calculator

Cal. Conc. TIMAP Conc. Normalization Ratio (2nd Run/diluted) Ratio (1st Run/stock)
25.87 3.603 0.139273289524546
14.362 3.606 0.251079236875087 1.80278097639703 0.93
65.623 5.726 0.0872559925635829
27.962 4.301 0.15381589299764 1.7628117963996 1.23

49.931 1.551 0.0310628667561235

114.79 2.837 0.0247146964021256 0.795634755676682 0.98
39.469 4 0.101345359649345
79.222 3.953 0.049897755673929 0.492353629673576 1.14
81.314 3.406 0.0418870059276385
34.239 3.409 0.0995648237390111 2.37698592998061 1.56

16.455 4.276 0.259860224855667

12.27 4.372 0.356316218418908 1.37118413799886 1.05
59.346 4.355 0.0733832103258855
72.945 4.782 0.0655562410034958 0.893341143190232 0.85

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