2024 HRPIWSProgram

Last updated
1/18/2024
Custom graphics designed by Emily Dung (NASA JPL)

Last Updated 1/18/2024
About the 2024 NASA HRP IWS graphics
Redefining What’s Possible
The winning graphic was designed by Emily Dung, an intern at NASA’s Jet Propulsion Laboratory. The graphics are used
in this program, the IWS website, mobile app and other tools, and in the presentation templates.
From the Artist:

The graphic is separated into three frames. The bottom frame reflects the research and work HRP has done in allowing
humans to explore space for extended periods of time. There are references to health and research that has been done
like Omics. This frame at the bottom is the foundation (research) for further exploration seen in the next two frames. The
middle frame shows the space launch system (SLS) going past the moon, somewhere we have been before. The top
frame shows the SLS breaking outside of the frame and reaching for Mars, showing where we will be going next.
The HRP IWS Steering Committee would like to thank all the graphic design submitters. There were several outstanding
submissions and it was a challenge to select one winner. The winning graphic, as well as the three (3) honorable
mentions will be on display throughout the workshop venue. Please take a moment to admire these amazing designs.
2024 NASA Human Research Program Investigators’ Workshop

February 13-16, 2024 15
NASA Human Research Program

Investigators’ Workshop
February 13-16, 2024
Galveston Island Convention Center

Galveston, Texas
Sponsored by:
NASA Human Research Program
Steering Committee
Maneesh Arya, Ph.D. Kris Lehnhardt, M.D.

David Baumann Jay Lemery, M.D.
Becky Brocato, Ph.D. Jancy McPhee, Ph.D.
Lisa Carnell, Ph.D. Lisa Milstead, M.S.
Lynn Clary, Ph.D. Cherie Oubre, Ph.D.
Rachael Dempsey Steven Platts, Ph.D.
Catherine Domingo Janapriya Saha, Ph.D.
Benjamin Easter, M.D. Michael Stenger, Ph.D.
Kristin Fabre, Ph.D. Judy Tate-Brown
Brian Gore, Ph.D. Nina Valverde, M.S.
Madison Hever Brandon Vessey, Ph.D.
Mohi Kumar Sara Whiting, Ph.D.
Charles Lloyd, Pharm.D. Alexandra Whitmire, Ph.D.
Jon McFather Janice Zawaski, Ph.D.

February 13-16, 2024 25
NASA Human Research Program Investigators’ Workshop

*all times are Central Time*
Tuesday, February 13
9:00 a.m. CT Exhibit Hall B Welcome and Remarks from the Human Research
Program (HRP) Chief Scientist – S Platts
9:10 a.m. Remarks from the HRP Program Director – D Baumann
9:20 a.m. Remarks from the Translational Research Institute for Space
Health (TRISH) Chief Scientific Officer – J Fogarty
9:40 a.m. Remarks from the NASA JSC Director – V Wyche
9:50 a.m. Remarks from the NASA Associate Administrator for the
Exploration Systems Development Mission Directorate –
C Koerner
10:10 a.m. Remarks from the NASA Chief Scientist – K Calvin
10:30 a.m. BREAK
10:45 a.m. Exhibit Hall B Space Radiation Element Welcome and Kick-off
Galleon Data Management
Grand Ballroom A Exploration Medical Capability Element: Overview
Grand Ballroom B Biomarkers: Central, Peripheral or Behavioral After
Exposure to Spaceflight Challenges
Grand Ballroom C Sensorimotor: Space Flight and Ground Analogs
12:15 p.m. Obtain Lunch (available for purchase, pre-function area)
12:45 p.m. Exhibit Hall B Lunch Plenary: International Panel – J McPhee
1:15 p.m. BREAK
1:30 p.m. Exhibit Hall B Plenary: The John B Charles Memorial Lecture – A Huang
2:30 p.m. BREAK
2:45 p.m. Exhibit Hall B Beyond the Mouse (Space Radiation)…
Galleon Commercial Spaceflight and Analogs
Grand Ballroom A Earth Independent Medical Operations (EIMO)
Grand Ballroom B Behavioral Health and Performance: Measures and
Methods
Grand Ballroom C SANS 1: Space Flight
4:15 p.m. BREAK
4:30 p.m. Exhibit Hall A Poster Session A
6:00 p.m. Adjourn
Wednesday, February 14
8:00 a.m. CT Pre-Function Area Morning Coffee Networking
9:00 a.m. Exhibit Hall B Biological Insights into Space Radiation Carcinogenesis
Galleon Miniature Marvels: Exploring Tissue Chips and
Organoids for Use in Space Health Research
Grand Ballroom A Artificial Intelligence Supporting Exploration Spaceflight
Health & Wellness
Grand Ballroom B Behavioral Health and Performance Over Time: Results
from Ground Analogs and Spaceflight
Grand Ballroom C SANS 2: Bed Rest
10:30 a.m. BREAK
10:45 a.m. Exhibit Hall B Plenary: Moon to Mars Program – A Kshatriya
11:45 a.m. Obtain Lunch (available for purchase, pre-function area)
12:15 p.m. Exhibit Hall B Lunch Plenary: Commercial Panel – J McPhee

February 13-16, 2024 35
12:45 p.m. BREAK

1:00 p.m. Exhibit Hall B Space Radiation Cross Risk Integrations Projects
Introduction
Galleon Joint Panel on the Role of the Vestibular Organs in
Space Exploration
Grand Ballroom A Point of Care Testing Supporting Exploration Spaceflight
Health
Grand Ballroom B Behavioral Health and Performance in Isolation and
Confinement: Outbrief from SIRIUS and HERA Missions
Grand Ballroom C SANS 3: Other Models
2:30 p.m. BREAK
2:45 p.m. Exhibit Hall A Poster Session B
4:15 p.m. BREAK
4:30 p.m. Pre-Function Area Speed Networking
6:00 p.m. Adjourn
Thursday, February 15
8:00 a.m. CT Pre-Function Area Morning Coffee Networking
9:00 a.m. Exhibit Hall B Behavioral Health in Rodent Models After Exposure to
Spaceflight Hazards
Galleon Masters of Metabolism for Long Duration Spaceflight
Grand Ballroom A IMPACT Health Risk Assessment Tool
Grand Ballroom B Characterizing and Mitigating Exploration Mission
Stressors
Grand Ballroom C Sensorimotor: Parabolic Flight
10:30 a.m. BREAK
10:45 a.m. Exhibit Hall B Plenary: In Memorium – M Arya
11:00 a.m. Plenary: Astronaut – K Lindgren
12:00 p.m. Obtain Lunch (available for purchase, pre-function area)
12:30 p.m. Exhibit Hall B Lunch Plenary: Other Government Agencies Panel – K
Fabre
1:00 p.m. BREAK
1:15 p.m. Exhibit Hall B Sex Differences in Biological Effects from Radiation
Exposure
Galleon Flight Research Operations & Integration
Grand Ballroom A Analysis & Risk Approaches To Exploration Spaceflight
Health
Grand Ballroom B Brain and Behavioral Health After Exposure to
Spaceflight Hazards – Results from Ground Analogs and
Spaceflight
Grand Ballroom C Advances in Food Nutrition, Acceptability, and
Microbiology for Spaceflight
2:45 p.m. BREAK
3:00 p.m. Exhibit Hall B Advanced Biological and Mathematical Techniques to
Understanding Space Radiation Damage
Galleon Isolation Analogs
Grand Ballroom A Long Term Health Metrics & Risks for Exploration
Spaceflight Health
Grand Ballroom B Human Capabilities Assessment for Autonomous
Missions: VNSCOR Outbrief
Grand Ballroom C Micro & Immune
4:30 p.m. BREAK

February 13-16, 2024 45
4:45 p.m. Ex Hall B/Pre-Func Area Career Development

6:00 p.m. Adjourn
Friday, February 16
8:30 a.m. CT Exhibit Hall B Space Radiation Central Nervous System Decrements
Galleon Space Biology: Plants in Space
Grand Ballroom A Pharmacy, Therapeutics & Metabolism in Exploration
Class Missions
Grand Ballroom B Human Factors Considerations for Future Missions
Grand Ballroom C Musculoskeletal Health and EVA Performance
10:00 a.m. BREAK
10:15 a.m. Exhibit Hall B Biological Effects of Combined Stressors
Galleon How We Do Business
Grand Ballroom A Exploration Crew Health and Performance
Grand Ballroom B Measuring and Supporting Resiliency and Adaptation
in Future Long Duration Exploration Missions
Grand Ballroom C Cardiovascular Responses to Varying Gravity
11:45 a.m. BREAK
12:00 p.m. Exhibit Hall B Awards and Closing Remarks – D Baumann, S Platts
Note: Exhibit Hall A (poster hall) layout is shown on the last page.

February 13-16, 2024 55
Tuesday, February 13, 2024
Space Radiation Element Welcome and Kick-off
10:45 AM Exhibit Hall B
Chairs: Janice Zawaski and Jason Weeks
10:45 AM Janice Zawaski; Jason Weeks

(1654194) SPACE RADIATION ELEMENT WELCOME AND STATE OF THE ELEMENT
The Space Radiation Element kick-off presentation to IWS 2024
11:10 AM Tony Slaba; Janice Huff; Steve Blattnig; Ryan Norman; Ianik Plante; Floriane Poignant;
Shirin Rahmanian; Charles Werneth; Xiaojing Xu
(1652068) SPACE RADIATION RISK MODELING - THE MULTI-MODEL ENSEMBLE RISK
ASSESSMENT PROJECT
The goal of the Multi‐model Ensemble Risk Assessment (MERA) project at NASA Langley
Research Center is to develop risk projection tools supporting robust and accurate evaluation
of space radiation health risks, including the assessment of medical countermeasures,
individualized risk for health care decisions, and combined spaceflight hazards.
11:25 AM Sigrid Reinsch; S. Robin Elgart; Peter Guida; Gregory Nelson; Janapriya Saha; Sergio Santa
Maria; Brock Sishc; Jason Weeks; Janice Zawaski
(1645749) NASA SPACE HEALTH IMPACTS FOR THE NASA EXPERIENCE (SHINE)
TRAINING PROGRAM: SPACE RADIATION CURRICULUM
In 2023 NASA rolled out its inaugural annual virtual Space Health Impacts for the NASA
Experience (SHINE) Space Radiation Didactic Curriculum which aims to educate participants
not only in the scientific aspects of space radiation but also in the agency’s risk management
strategies
11:33 AM Shelita Augustus; Janice Zawaski; Angela Kubik
(1693071) INTRODUCTION TO THE FUTURE OF THE NEW THREE WEBSITE
Historically the THREE website (https://three.jsc.nasa.gov/) was an encyclopedic site whose
goal is to present a discussion of the space radiation environment and its health risks to
humans. It is our goal in 2024 to update the website to not only be an encyclopedic site but
to increase its scope to include areas such as funding opportunities, grantsmanship, job
opportunities, etc..
11:41 AM Peter Guida; Trevor Olsen
(1643971) NSRL USER GROUP: BIOLOGY, PHYSICS & OPERATIONS
This presentation will serve as the annual NSRL user group forum.
12:06 PM Discussion
12:15 PM Obtain Lunch (available for purchase in the pre-function area)

February 13-16, 2024 65
Data Management
10:45 AM Galleon
Chairs: Olga Kieschnick and Kathryn Richards

10:45 AM Chair Remarks
10:50 AM Deepak Kulkarni; Susan Steinberg; Greg Eley; Macresia Alibaruho
(1643679) HRP DATA MANAGEMENT PLAN
This presentation will discuss new HRP data management policies and procedures.
11:04 AM Daniel Berrios; Gregory Eley; Ashley Mooney; Deepak Kulkarni
(1645678) ACQUISITION OF AND ACCESS TO RESEARCH OMICS DATA
To assure maximum benefit from omics data, the NASA Human Research Program Data
Management Plan stipulates that human omics data should be archived within and accessed
through the NASA Life Sciences Portal (NLSP).
11:18 AM Kathryn Richards; Byron Smith
(1645482) KNOWLEDGE IS POWER AND DATA STEWARDSHIP IS A SUPERPOWER
With knowledge management, data stewards are the key to implementing governance and
maximizing high value results to ultimately solve some of NASA’s most persistent data
challenges while providing opportunities to close gaps in its ability to make data-backed
decisions.
11:32 AM Jessica Keune; Robert Beaton; Diedre Thomas
(1643787) UPDATES TO THE RESEARCH DATA SUBMISSION AGREEMENT (RDSA)
BLANKBOOK
This presentation will discuss the comprehensive updates that have been made to the
Research Data Submission Agreement (RDSA) Blankbook, a key document in the life cycle
of a Human Research Program (HRP)-funded investigation.
11:46 AM Ryan Scott; Sylvain Costes; Samrawit Gebre; Danielle Lopez; Lauren Sanders; Amanda
Saravia-Butler
(1645895) THE NASA OPEN SCIENCE DATA REPOSITORY: BIOMEDICAL FAIR DATA,
ANALYSIS TOOLS, USER COMMUNITIES, PUBLICATIONS, AND DISCOVERIES FOR
DEEP SPACE MISSIONS
The NASA Open Science Data Repository is FAIR, maximally open access, has data from
>450 studies from model organisms and non-NASA human astronauts, ~600 Analysis
Working Group members data mining, enabled >60 publications, and offers public users the
ability to upload, download, analyze, and visualize data across ‘omics, physiological,
behavioral, bioimaging, video, and environmental telemetry.
12:00 PM Discussion

February 13-16, 2024 75
Exploration Medical Capability Element: Overview
10:45 AM Grand Ballroom A
Chair: Nancy Fleming

10:45 AM Kris Lehnhardt; Michael Krihak; Jay Lemery; Benjamin Easter
(1651956) THE EXPLORATION CREW HEALTH AND PERFORMANCE SYSTEM OF THE
FUTURE - A SHARED MENTAL MODEL
This presentation will outline the initial work that Exploration Medical Capability is leading on
the development of a prototype design for an exploration Crew Health and Performance
(CHP) system for future NASA human deep space long-duration missions.
11:00 AM Moriah Thompson; Rahul Suresh; Justin Yang
(1646800) AN INTEGRATED ROADMAP FOR ADDRESSING EXPLORATION MEDICAL
CAPABILTY GAPS
An overview of the NASA Exploration Medical Integrated Product Team (XMIPT)'s integrated
roadmap to close human health gaps for exploration missions.
11:15 AM Michael Krihak; John Odina
(1646921) EXMC SYSTEMS ENGINEERING STATUS
This presentation will provide a high-level overview of what the ExMC SE team has
accomplished since the last Investigators’ Workshop, an introduction to upcoming SE talks,
and the ongoing systems engineering work.
11:30 AM Michael Krihak; Christopher Laing; John Odina
(1646946) 2023 ARTEMIS CREW HEALTH AND PERFORMANCE SYSTEM MODEL
DEVELOPMENT
By using a Model-Based Systems Engineering (MBSE) approach, existing requirements,
functions, and concepts of operations were combined to create a single system model
focused on representing CHP from the launch to the return to Earth segments of the Artemis
III and IV missions.
11:45 AM Jordan Blackwelder; Marina Parker; Michael Krihak; John Odina
(1646975) ARCHITECTURAL FRAMEWORK FOR CONCEPTUALIZING EXPLORATION
CLASS MEDICAL OPERATIONS
Development of a System of Systems Architecture (SoSA) utilizing model-based systems
engineering (MBSE) to capture various independent but related efforts into a comprehensive
view associated with the goal of providing progressively Earth-Independent Medical
Operations (EIMO).
12:00 PM Discussion

February 13-16, 2024 85
Biomarkers: Central, Peripheral or Behavioral After Exposure to Spaceflight Challenges
10:45 AM Grand Ballroom B
Chairs: Catherine Davis and Jimmy Zaid

10:45 AM Catherine Davis; Victoria Elliott; Joan Smith; Sofia Manicka
(1648039) THE EFFECTS OF PROTON RADIATION ON ATTENTION, MEMORY, AND SOCIAL
AND ANXIETY-LIKE BEHAVIOR IN MALE AND FEMALE RATS
Male and female rats were exposed to proton radiation and assessed for changes in sustained
attention, social memory, and social and anxiety-like behaviors at different time points following
exposure, and these results were compared to the effects of exposure to the sim GCR sim on similar
behavioral endpoints.
10:57 AM Gregory Nelson; Tamako Jones; Seta Stanbouly
(1645630) EFFECTS OF RADIATION DOSE RATE ON THE MOUSE NERVOUS SYSTEM
The effect of simulated galactic cosmic rays on the mouse brain was evaluated with a battery of
behavioral, network function and gene expression measures. The effects of dose rate were
determined and two scaling parameters for risk assessment were derived: dose rate effectiveness
factor (DREF) and relative biological effectiveness (RBE). Male and female 6 mo-old C57Bl/6 mice
were the model.
11:09 AM April Ronca; Siddhita Mhatre; Janani Iyer; Steffy Tabares Ruiz; Stephanie Puukila; John Hayes;
Adaline Brekker; Marissa Burke; Maya Semel; Moniece Lowe; Linda Rubinstein; Marianna Sowa;
Yasaman Shirazi-Fard; Candace Tahimic; Joshua Alwood; Amber Paul
(1652029) PLASMA AND BRAIN CYTOKINE RESPONSES TO SINGLE AND COMBINATORIAL
SPACEFLIGHT STRESSORS IN MALE AND FEMALE MICE
Plasma and brain cytokine cytokine profiling of crew age-matched mice exposed to either 5-ion
GCRsim alone or in combination with hindlimb unloading/head down tilt revealed sex-specific, as well
as sex-common, responses with plasma granulocyte colony stimulating factor (GCSF) emerging as a
potential biomarker.
11:21 AM Andrew Wyrobek; I. Bansal; R.A. Britten; J.B. Brown; R.B. Gunier; A.M. Mora; B.M. Rabin; S. Rauch;
L.M. Sanders; A.M. Saravia-Butler; T. Straume; H.E. Witkowska
(1705278) MOLECULAR MODELING OF SUSCEPTIBILITY FOR ANXIETY, MEMORY, AND
CLEVERNESS BEHAVIORS AMONG MALE RATS EXPOSED TO SPACE-RELEVANT RADIATION
Modeling of CNS proteins expression showed quantitative relationships to three distinct radiation-
sensitive behaviors (EPM-anxiety, BM-memory, BM-cleverness) across sham and irradiated outbred
male rats, with similar findings for hippocampal and blood transcripts, with associations to abnormal
brain tissue functions and with concurrence to publicly available datasets for similar behaviors.
11:33 AM Richard Simpson; Kyle Smith; Elizabeth Beattie; Forrest Baker; Grace McKenzie; Alice Lagoy; Brian
Crucian; Judith Gordon; Christopher Connaboy
(1673174) PROMOTING BEHAVIORAL HEALTH, COGNITIVE, SENSORIMOTOR, AND IMMUNE
FUNCTION USING GUIDED IMAGERY TO AUGMENT EXERCISE TRAINING IN AN ISOLATED
AND CONFINED SPACEFLIGHT ANALOG ENVIRONMENT
Promoting behavioral health, cognitive, sensorimotor, and immune function using guided imagery to
augment exercise training in an isolated and confined spaceflight analog environment.
11:45 AM Namni Goel; Lauren Pasetes
(1607258) BIOMARKERS AS PREDICTORS OF RESILIENCY AND SUSCEPTIBILITY TO STRESS
IN SPACE FLIGHT
The aim of this project is to validate biomarkers as predictors of susceptibility or resiliency to the
neurobehavioral effects of stress and sleep deprivation for use in space flight in short-duration and
long-duration analogs.
12:00 PM Discussion
February 13-16, 2024 95
Sensorimotor: Space Flight and Ground Analogs
10:45 AM Grand Ballroom C
Chairs: Scott Wood and Tim Macaulay

10:45 AM Sarah Moudy; Scott Wood; Brian Peters; Torin Clark; Michael Schubert; Austin Bollinger; Yiri
De Dios
(1646480) VALIDATION OF A SENSORIMOTOR DISORIENTATION GROUND ANALOG
The purpose of this study was to validate a ground-based Sensorimotor Disorientation
Analog (SDA), that was previously developed based on subjective feedback from previously
flown astronauts, by comparing performance in movement tasks that have a wealth of
spaceflight data against task performance while using the SDA.
11:00 AM Michael Schubert; Scott Wood; Colin Grove; Danny Ludwig; Darci Gallimore; Kendra Carter;
Richard Folga; Adam Preston; C. Matthew Stewart
(1650636) SELF-ADMINISTERED REHABILITATION TOOL TO MITIGATE MOTION
SICKNESS AND ENHANCE SENSORIMOTOR RECOVERY
The self-administered SWAN rehabilitation tool appears to offer a quicker recovery than
traditional therapy for some of the common signs and symptoms related to having a
unilateral vestibular deficit.
11:15 AM Torin Clark; Taylor Lonner; Aaron Allred; Aadhit Gopinath; Charles Oman; Ben Lawson; Eric
Groen; Paul DiZio
(1652139) ASSESSMENT OF COUNTERMEASURES TO MITIGATE MOTION SICKNESS
FOR POST-FLIGHT ASTRONAUTS DURING WATER LANDINGS
In ground-based analog experiments, we have evaluated non-pharmacological
countermeasures, including providing visual cues of self-motion in a head mounted display
and engaging postural control mechanisms, to help mitigate motion sickness relevant for
astronauts during water landings.
11:30 AM Olga Kuldavletova; Gilles Clement; Deborah Navarro Morales; Timothy Macaulay; Scott
Wood; Pierre Denise
(1643001) ASTRONAUTS COGNITIVE AND BALANCE FUNCTIONS AFTER
SPACEFLIGHT ARE COMPARABLE TO THOSE OF PATIENTS WITH BILATERAL
VESTIBULAR LOSS
Functional deconditioning of astronauts inflight or after space flight in balance and cognitive
tasks is similar to the performance of patients with bilateral vestibular deficits, which might
indicate the vestibular role in this deconditioning and open the possibility of using vestibular
deficient patients as a model of astronauts for the development of countermeasures and
adaptation strategies.
11:45 AM Aaron Allred; Torin Clark
(1647634) MODELING HUMAN SELF-ORIENTATION PERCEPTION RESTULTING FROM
GALVANIC VESTIBULAR STIMULATION AND PHYSICAL MOTION
We provide a model of spatial orientation perception, based on the observer model, with
neuronal activity encoding canal sensory transduction modulated by GVS currents.
12:00 PM Discussion

February 13-16, 2024 105
Beyond the Mouse (Space Radiation)…
2:45 PM Exhibit Hall B
Chairs: Steve Blattnig and Janice Zawaski

2:45 PM Christopher Yan; Celine Lai; Sushma Shenoy; Rebecca Yu; Zachary Cook; Julio Guevara;
Yan Zhuge; Joseph Wu; Paul Pang
(1651907) DERIVATION OF LARGE BIOREPOSITORY OF HUMAN IPSC LINES FOR
OPEN ACCESS SHARING AMONG ACADEMIC INVESTIGATORS
We introduce the Greenstone Biosciences iPSC Biobank, a biorepository containing >1,000
human iPSC lines with de-identified patient clinical information and genetic backgrounds.
3:05 PM Afshin Beheshti; Bianca Cerqueira; Holly Chapapas; Giovanni Lorenz; Paul Sherman; Diego
Galeano
(1645464) PREDICTING PREDISPOSED SPACE RADIATION HEALTH RISKS FROM
GRAVITY AND HYPERBARIC ALTERATIONS DETERMINED FROM MINI-PIGS STUDIES
We were able to determine a conserved set of miRNAs between the minipigs and spaceflight
rodents which targeted mitochondrial suppression due to the hypobaric conditions.
3:20 PM Sarah Hay; Timothy Boorady; Rina Eden; Nina Porter
(1626719) TRANSIENT ACUTE LIVER INFLAMMATION DURING FRACTIONATED X-RAY
IRRADIATION WITHIN PARAMETERS OF A LUNAR MISSION: A PORCINE MODEL
We exposed 6 Yucatan minipigs to different doses of an 8- week fractionated x-ray series
totaling 0.1 Gy to the liver then examined the livers s/p necropsy and compared
histopathologic findings to the livers of the 6 control pig.
3:35 PM Lawrence Dauer; Kathryn Held; John Boice
(1643947) THE U.S. MILLION PERSON STUDY OF LOW-DOSE-RATE HEALTH
EFFECTS: INNOVATIONS IN EVALUATING COGNITION AND MULTIPLE STRESSORS
Investigating cognition outcomes in the Million Person Study as well as an epidemiologic
study of 170,000 nuclear submariners for late-occurring neurological and behavioral
disorders and persistent cognitive impairment related to multiple stressors experienced
concurrently with chronic radiation exposure for possible interaction between radiation and
multiple stressors as a focus.
4:05 PM Discussion
4:15 PM Break

February 13-16, 2024 115
Commercial Spaceflight and Analogs
2:45 PM Galleon
Chairs: Emmanuel Urquieta and Jimmy Wu

2:45 PM Chair Remarks
2:50 PM Nick Downs; Bryan Eleogram; Jason Sylva
(1645264) NEVADA SPACE PROVING GROUNDS AT THE NEVADA NATIONAL
SECURITY SITE: CAPABILITIES, CHALLENGES, AND OPPORTUNITIES WITH A
MISSION-SCALE, HEAVILY CRATERED TESTBED
The Nevada Space Proving Grounds were extensively used by Apollo astronauts to train in a
heavily cratered, volcanic terrain; NSPG's utilization for upcoming Artemis missions is an
important de-risking step for NASA and NASA's commercial, academic, and national lab
partners.
3:02 PM Jeffrey Ayton; John Cherry
(1647988) AUSTRALIAN ANTARCTIC SPACE ANALOG
The Australian Antarctic Program is a hi-fidelity space analogue featuring small, confined,
isolated populations, with real environmental hazards, and a proven medical support model.
Recent collaboration with TRISH provides opportunity to utilise the Australian Antarctic
Space Analog to inform advances in healthcare on long duration exploratory space missions
and translate to terrestrial benefit.
3:14 PM Marc Shepanek
(1645410) A BRIEF EVOLUTION OF GROUND BASED ANALOGS IN SUPPORT OF
SPACED MISSIONS
To better address developing missions to the Moon and Mars, ground based analogs have
and are, providing a range of opportunities for humans to; deal with extreme environments,
adapt to extreme environments, and return to their home environments. Whether for
research or technology development,ground based analogs have been, and are, a critical
element for human space exploration.
3:26 PM Jonathan Bennett; Jeff Ayton; Sandra Iuliano; Nisa Kelly; John Cherry; Michael Wong; Kate
Kloza; Lambert Leong; Bosco Li; Yong Liu; Aenor Sawyer; Aenor Sawyer; John Shepherd
(1618032) EVALUATION OF BODY SHAPE AS A HUMAN BODY COMPOSITION
ASSESSMENT IN ISOLATED CONDITIONS AND REMOTE ENVIRONMENTS
The development and application of optimal configurations of 3-dimensional optical imaging
for body composition assessment allows for the monitoring of changes in fat and fat-free
mass composition in remote space analogs
3:38 PM Doug Ebert; Jeff Ayton; Theodora Bock; John Cherry; Aaron Everson; John Martin; Jaime
Mateus; Victoria Perizes; Marissa Rosenberg; Ashot Sargsyan; Emmanuel Urquieta; Jimmy
Wu
(1647918) SIMULTANEOUS EVALUATION OF SKILL MANAGEMENT METHODS AND
PHYSIOLOGY USING AN INTUITIVE HANDHELD ULTRASOUND SYSTEM IN
SPACEFLIGHT AND AN ANTARCTIC SPACEFLIGHT ANALOG
These projects examine both ultrasound skill management methods and physiology using a
handheld ultrasound device on the Polaris Dawn mission and at Australian Antarctic Division
stations.

February 13-16, 2024 125
3:50 PM Emmanuel Urquieta; Mathias Basner; Harshavardhan Doddapaneni; Amoy Fraser; Richard
Gibbs; Michal Masternak; Stephen Mayo; Jimmy Wu
(1650313) EXPAND SPACE OMICS SAMPLE COLLECTION: A MULTI-INSTITUTION
CONSORTIUM
The EXPAND Space Omics Program is one-of-a-kind gold-standard sample collection and
processing capability to enable research in commercial spaceflight. Given the complexity of
having different sites for training, launch, and landing of commercial spaceflight participants,
TRISH/EXPAND has developed a consortium of institutions across the United States to
ensure that samples keep their quality.
4:02 PM Discussion
4:15 PM Break

February 13-16, 2024 135
Earth Independent Medical Operations (EIMO)
2:45 PM Grand Ballroom A
Chair: Jay Lemery

2:45 PM Jay Lemery; Ben Easter; Kris Lehnhardt
(1682243) EARTH INDEPENDENT MEDICAL OPERATIONS
EIMO is the gradual transition of medical care and decision making from terrestrial to space-
based assets, enabling support of astronaut health and performance and reducing overall
mission risk.
3:00 PM Marina Parker; Michael Krihak; Christopher Laing; Jorge Bardina; Arian Anderson
(1646978) EARTH-INDEPENDENT MEDICAL OPERATIONS (EIMO) CONCEPT OF
OPERATIONS
The Human Research Program (HRP) Exploration Medical Capability (ExMC) Element has
developed a model-based Concept of Operations outlining an initial vision for Earth-
Independent Medical Operations Medical System providing illustrative examples of the
various activities (scenarios) for which the system will be employed during Mars missions.
3:15 PM Kurt Berens; Ben Easter; Michael Krihak; Kris Lehnhardt; Jay Lemery
(1661383) EARTH INDEPENDENT MEDICAL OPERATIONS (EIMO) DATASCOPE:
CHALLENGES AND POTENTIAL SOLUTIONS
This abstract summarizes findings from a Technical Interchange Meeting conducted 21
August 2023 surrounding the anticipated data environment necessary to enable
progressively earth independent medical operations.
3:30 PM Behnood Gholami; Negar Ebadi
(1661261) ASTROSIGHT: A REAL-TIME REMOTE CARDIOPULMONARY SENSING
SYSTEM FOR HUMAN EXPLORATION MISSIONS
We present our ongoing work on developing and validating a non-invasive real-time
cardiopulmonary monitoring system.
3:45 PM Lauren Sanders; Sylvain Costes; Ryan Scott
(1650291) BIOLOGICAL RESEARCH AND SPACE HEALTH ENABLED BY MACHINE
LEARNING TO SUPPORT DEEP SPACE MISSIONS
Here we present a decadal view of AI/ML architecture to support deep space mission goals,
developed in concert with leaders in the field.
4:00 PM Discussion
4:15 PM Break

February 13-16, 2024 145
Behavioral Health and Performance: Measures and Methods
2:45 PM Grand Ballroom B
Chairs: Mathias Basner and Katherine Rahill

2:45 PM Mathias Basner; David Dinges; Adrian Ecker; Ruben Gur; Christopher Jones; Tyler Moore;
David Roalf; Kosha Ruparel
(1648815) NASA'S COGNITION TEST BATTERY FOR SPACEFLIGHT: 10-YEAR REVIEW
AND FUTURE PERSPECTIVES
NASA's Cognition test battery is a widely validated and used neuropsychological test battery
specifically designed for high-performing astronauts. Its 10 test cover a range of cognitive
domains with established brain network recruitment via fMRI. This presentation provides an
overview of Cognition development, key study findings and a perspective on the future of
Cognition.
3:00 PM Jennifer Miller; Suzanne Bell; Lauren Landon
(1647577) DEVELOPMENT AND VALIDATION OF SHORT-FORM EXPLORATION
MEASURE SURVEYS
We describe validation efforts of shorter versions of standard surveys for deployment in
analogs and/or spaceflight.
3:15 PM Alaa Khader; Sheena Dev; Steven Anderson; Suzanne Bell
(1644017) A COMPARISON OF HEART RATE VARIABILITY ON HIGH AND LOW STRESS
TASKS IN ISOLATED, CONFINED AND CONTROLLED SETTINGS
Comparing heart-rate data collection protocols in in the Human Factors and Behavioral
Performance Exploration Measures, and assessing the utility of HRV as an unobtrusive
biomarker of stress adaptability in isolated, confined, and controlled settings.
3:30 PM Theodora Chaspari; Sydney Begerowski; Suzanne Bell; Alaa Khader; Projna Paromita
(1645411) SPEECH-BASED ARTIFICIAL INTELLIGENCE FOR DETECTING MICRO-
BEHAVIORS AND PREDICTING THEIR EFFECT ON TEAM PERFORMANCE
We investigate micro-behaviors in team conversations and their association with
operationally-relevant team performance. The three aims of our work are: 1) Leverage
speech-based data analytics and artificial intelligence to detect microbehaviors in team
interactions; 2) Investigate the impact of microbehaviors on team performance; and 3)
Design machine learning systems to predict team performance.
3:45 PM Suzanne Bell; Aaron Schecter; Alla Vinokhodova; Vadim Gushin; Leslie DeChurch; Noshir
Contractor
(1648245) TEAM COMPOSITION AND INTERPERSONAL RELATIONSHIP FORMATION
IN ISOLATED AND CONFINED ENVIRONMENTS
We collected data from 9, 4-person HERA crews in isolation for up to 45 days, developed
and tested a model of interpersonal relationship formation, and examined the validity of a
method for assessing interpersonal compatibility.
4:00 PM Discussion
4:15 PM Break

February 13-16, 2024 155
SANS 1: Space Flight
2:45 PM Grand Ballroom C
Chairs: Steve Laurie and Tyson Brunstetter

2:45 PM* Steve Laurie; Brandon Macias
(1644808) SPACEFLIGHT ASSOCIATED NEURO-OCULAR SYNDROME (SANS)
RESEARCH UPDATE
Structural changes of the eye and brain are now well-established findings of spaceflight
associated neuro-ocular syndrome (SANS) affecting the majority, but not all, of
crewmembers flying ~4–6-month standard duration missions to the International Space
Station (ISS). We will present advancements in SANS research from both spaceflight and
analog studies, and provide an update on countermeasures.
2:45 PM* Tyson Brunstetter; Sara Mason; Suzi Osborne; C. Robert Gibson; Mary Van Baalen; Ann
Tsung; William Tarver
(1654829) SPACEFLIGHT ASSOCIATED NEURO-OCULAR SYNDROME (SANS): 2024
CLINICAL UPDATE - EYES & VISION
An update will be provided on the latest Spaceflight Associated Neuro-ocular Syndrome
(SANS) clinical analyses and insight.
3:08 PM Mark Christopher; Jalil Jalili; Robert Weinreb; Steven Laurie; Brandon Macias; Alex Huang
(1642467) PREDICTING SANS USING ARTIFICIAL INTELLIGENCE
Deep learning artificial intelligence (AI) models were trained to predict space associated
neuro-ocular syndrome (SANS) risk based on optical coherence tomography (OCT) imaging.
Even with limited OCT data for training, models were able to accurately predict development
of SANS using only OCT data collected pre-flight.
3:21 PM Floris Wuyts; Diana Giraldo; Ben Jeurissen; Steven Jillings; Ekaterina Pechenkova; Viktor
Petrovichev; Ilya Rukavishnikov; Elena Tomilovskaya; Angelique Van Ombergen; Peter zu
Eulenburg
(1646716) WHITE MATTER CHANGES AFTER LONG-DURATION SPACEFLIGHT: NEW
INSIGHTS
This study is the first fixel-based analysis of diffusion MRI data in cosmonauts and it shows
widespread changes in the white matter after spaceflight, which are predominantly
macroscopic artefacts rather than microscopic changes, while in the cerebellum evidence of
new white matter sprouting is elucidated.
3:34 PM Steven Jillings; Chloe Mohanadass; Lauren Church; Chloë De Laet; Inna Nosikova; Elena
Tomilovskaya; Ilya Rukavishnikov; Victor Petrovichev; Ekaterina Pechenkova; Peter zu
Eulenburg; Floris Wuyts
(1650031) VENTRICULAR VOLUME CHANGES ACROSS MULTIPLE SPACEFLIGHTS
Brain ventricular volumes were calculated from cosmonauts' MRI scans before and after at
least two consecutive missions to the ISS, leading to a long follow-up study of ventricular
volume change as a result of spaceflight.
3:47 PM Katherine Warthen; Stuart Sater; Larry Kramer; Khader Hasan; Michael Williams; Brandon
Macias; Steven Laurie; Bryn Martin
(1647025) LONG-DURATION SPACEFLIGHT ALTERS INTRACRANIAL TISSUE AND
FLUID POSITION
Long-duration spaceflight is associated with a consistent and quantifiable whole brain shift
upward, along with corresponding opposite shift in the surrounding CSF.
4:00 PM Discussion
4:15 PM Break
* Single presentation for multiple abstracts

February 13-16, 2024 165
Poster Session A: Bone
Poster Hall Location 4:30 PM Exhibit Hall A

165 Margaret Wydotis; Lance Bollinger; John Caruso; Neel Patel; Katherine Maguire
(1621740) EXERCISE-INDUCED STRAIN AND NUTRITIONAL STRATEGIES TO ABATE
IN-FLIGHT BONE LOSS
The abstract describes exercise- and nutritional- based treatments to abate inflight bone
loss.
166 Jean Sibonga; Robert Adler; Chang Gregory; Ashraf Gorgey; Millennia Young; Dimitri Martel
(1636465) MAGNETIC RESONANCE IMAGING (MRI) TO ASSESS CHANGES TO
TRABECULAR MICROARCHITECTURE OF THE HIP
This study validates the sensitivity of 3T MRI to detect irreversible losses of connectivity in
the hip trabecular bone following 12 months of non-weightbearing in newly admitted patients
following spinal cord injury.
167 Richard Perkins; Raj Prabhu; Elisabeth Spector; Jean Sibonga
(1643220) QUANTIFYING FRACTURE PROBABILITIES FOR FUTURE SPACE MISSIONS
USING COMPUTATIONAL METHODOLOGIES
Using computational models and various imaging techniques, bone fracture risk
assessments may be performed for conditions relevant during future Artemis missions to the
Moon and Mars.
168 Anna Wadhwa; Courtney Mazur; Katelyn Strauss; Jennifer Coulombe; Charles Fuller; Satoru
Takahashi; Martha Hotz-Vitaterna; Mary Bouxsein; Marc Wein
(1644405) SINGLE CELL TRANSCRIPTOMICS OF FEMORAL BONE MARROW FROM
RODENTS EXPOSED TO ARTIFICIAL GRAVITY ON THE INTERNATIONAL SPACE
STATION: A NASA-JAXA COLLABORATION
Single-cell transcriptomics of femoral bone marrow revealed significant heterogeneity from
spaceflight (0g) and artificial gravity (1g, using the JAXA Multiple Artificial-gravity Research
System[MARS]), pointing to differential responses in immune function, metabolism,
erythropoiesis, bone matrix mineralization, and more.
169 Anna-Maria Liphardt; Elie-Tino Godonou; Annegret Mundermann; Nadja Djalal; Maren
Dreiner; Frank Zaucke; Wolfgang Wirth; Anna Wisser; Susanne Maschek; Felix Eckstein;
Georg Schett; Anja Niehoff
(1646057) KNEE CARTILAGE QUALITY AND TYPE II COLLAGEN METABOLISM IN
RESPONSE TO 21-DAYS BED REST IMMOBILIZATION
This study aims to investigate the effects of 21 days of head-down-tilt bed rest (HDT) (with
and without exercise and nutrition interventions) on type II collagen metabolism and on knee
cartilage quality in healthy male study participants.
170 Chirayu Patel; Sabrina Vander Wiele; Kaitlyn Reno; Leslie Kim; Jennifer Coulombe; Charles
Fuller; Satoru Takahashi; Martha Vitaterna; Mary Bouxsein; Jeffrey Willey
(1646698) ARTHRITIC REPONSES OF MOUSE KNEE MENISCI TO ARTIFICIAL GRAVITY
ABOARD THE INTERNATIONAL SPACE STATION
1g of artificial gravity aboard the ISS prevented pro-arthritic pathway enrichment and
chondrocyte apoptosis, but exposure to lower g-levels resulted in pro-arthritic transcriptomic
changes similar to those seen at µg.
171 Benjamin Hezrony; Philip Brown
(1648410) A FAST LEVEL SET APPROACH TO MICROSTRUCTURAL INVERSE DESIGN
This novel work presents the first occurrence of a fast structural optimization algorithm with
sub-integration point accuracy, used to print 3D microstructures with equivalent pure linear
elastic anisotropy to targeted human skeletal microstructures derived via GPU accelerated
FEA homogenization.

February 13-16, 2024 175
Poster Session A: Cardiovascular

039 Hyelim Park; Bokyeong Park; Hyewon Park; Junbeom Park; Kyu-Sung Kim
(1637212) ANTI-ARRHYTHMIA EFFECT OF INTERMITTENT HYPOXIA THROUGH
ATRIAL FIBRILLATION REMODELING
This study aimed to find the relationship of atrial fibrillation in acute Intermittent hypoxia and
investigate its potential as a therapeutic target in AF.
081 Hrudayavani Vellore; Ana Diaz Artiles; Raquel Galvan-Garza
(1641686) USING COMPUTATIONAL PHYSIOLOGICAL MODELING TO DEVELOP A
ROBUST DATASET FOR DEVELOPMENT OF HUMAN ASSESSMENT ALGORITHMS
Using computational physiological modeling of the cardiovascular and respiratory systems to
develop a robust dataset of various gravitational profiles for a large representative population
of individuals for use by future machine learning algorithms.
082 Renee Abbott; Annelise Miller; David Martin; Chris Miller; Jason Lytle; Steven Laurie; Stuart
Lee; Ana Diaz-Artiles; Brandon Macias
(1644331) SEMI-AUTOMATIC EXTRACTION OF INTERNAL JUGULAR VEIN PRESSURE
USING SONOGRAPHY COMPRESSION
A new methodology to extract IJV pressure values frame by frame from video recordings of
compression sonography
040 Sanal Kumar V R
(1646500) ELEVATED BLOOD VAPOR PRESSURE AND THE REDUCED HEAT
CAPACITY OF BLOOD LEAD TO GAS EMBOLISM CAUSING FLOW CHOKING AT A
CRITICAL PRESSURE RATIO IN BOTH GRAVITY AND MICROGRAVITY
ENVIRONMENTS
Maintaining a blood pressure ratio below the critical pressure ratio is crucial to prevent flow
choking in gravity/microgravity environments. Keeping diastolic BP higher than vapor
pressure reduces gas embolism risks. Increasing blood heat capacity lowers vapor pressure,
delaying flow choking and reducing cardiovascular risks. Our discovery significantly
contributes to increasing human longevity.
083 Syeda Zaman; Matteo Fois; Stefania Scarsoglio; Luca Ridolfi; Ana Diaz-Artiles
(1646513) CARDIOVASCULAR AND OCULAR CHANGES OBSERVED DURING 60
MINUTES OF HEAD-DOWN TILT
In this study, we propose to characterize cardiovascular and ocular changes during and after
60 minutes of 6° head-down tilt to simulate altered gravity.
084 Richard Whittle; Ana Diaz-Artiles
(1648651) COMPUTATIONAL MODELING OF CARDIOVASCULAR DOSE-RESPONSE
CURVES IN ALTERED-GRAVITY
In this study, we present data from computational modeling of the cardiovascular system to
augment and extend predictions of dose-response curves derived from experimental data.
085 Stuart Lee; David Martin; Christine Ribeiro; Sondra Perez; Scott Smith; Sara Zwart; Connor
Ferguson; Steven Laurie; Brandon Macias
(1648664) INDICES OF CARDIOVASCULAR DISEASE RISK IN ASTRONAUTS AFTER
LONG-DURATION SPACEFLIGHT IN LOW EARTH ORBIT
The objective was to measure arterial structure and function in these crewmembers for up to
5 years after returning to Earth to determine if long-term negative sequelae develop.

February 13-16, 2024 185
086 Kole Lutz; Terry Trevino; Tara Nibhanupudy; Terry Rector

(1649220) EFFECTS OF ELECTROMAGNETIC FIELDS ON RED BLOOD CELLS, FLUID
SHIFT, AND STASIS
Effects of Pulsed Magnetic Field on the RBCs, blood viscosity, and shear rate are outlined.
As too much iron or problems with utilising, storing, or transporting iron is the leading cause
of high blood pressure, PEMF also provides △v to move more ferrous minerals such as
Fe3O4 in red blood cells. A lightweight PEMF wearable is tested to move cells and fluids for
Earth and space.
087 Bokyeong Park; Hyelim Park; Kyu-Sung Kim; Hyewon Park; Junbeom Park
(1649222) THE ROLE OF MICROGRAVITY IN REGULATING AQUAPORIN 4 CHANNELS
RELATED TO ATRIAL REMODELING IN ATRIAL FIBRILLATION.
The aim of this study was to find the relationship between AF and AQP4 expression at the in
vitro level and to investigate the potential of Microgravity and AQP4 as therapeutic targets in
AF.
088 Jason Lytle; Stuart Lee; Steven Laurie; Matthew Poczatek; Annelise Miller; Cambria
O'Grady; Connor Ferguson; Stephanie Melvin; Brandon Macias
(1649835) EVALUATING EFFICACY OF END-OF-MISSION FLUID LOADING
PROTOCOLS
The purpose of this study is to test the efficacy and palatability of the two fluid loading
protocols ingested using the Russian and/or NASA timing protocols.
089 Andrew Blaber; Kouhyar Tavakolian; Da Xu
(1649920) 6-MONTH SPACEFLIGHT IMPAIRS CARDIORESPIRATORY RESPONSES TO
EXERCISE AND ORTHOSTATIC STRESS
Overall reduction of cardiorespiratory controls was observed in four astronauts upon return to
Earth after 6-month spaceflight in response to a supine-to-stand test and cycling exercise
from data collected by the Bio-M garment.
090 Manuel Albornoz-Miranda; Marcela Abde-Celis; Carlos Abarca-Araya
(1650645) MICROGRAVITY AS A RISK FACTOR FOR VENOUS THROMBOEMBOLIC
EVENTS IN ASTRONAUTS: PROPOSED PATHOPHYSIOLOGY
Hypercoagulability, blood stasis, and endothelial stress might occur in a microgravity
environment, leading to thromboembolic events, with inter-individual variability.
091 Mattias Neset; Svetlana Komarova; Anand Narayanan; Ryan Scott
(1650671) CITIZEN SCIENCE APPROACH FOR SEARCHING AND CURATING
LITERATURE ON THE EFFECTS OF SPACEFLIGHT ON CARDIOVASCULAR
OUTCOMES IN RODENTS AND HUMANS
We used a citizen science approach to rapidly compile and analyze large-scale
cardiovascular spaceflight data while promoting spaceflight research in several groups within
the community.
092 Jhan Saavedra Torres; Maira Alejandra Guayambuco Medina
(1652555) CARDIOVASCULAR DECONDITIONING: PROLONGED EXPOSURE TO
MICROGRAVITY REDUCES CARDIAC CONTRACTILITY AND ENHANCES OTHER
MECHANISMS
Simulated microgravity (SMG) causes ROS production in human cardiomyocytes (CMs).
Exposure to outer space microgravity poses a risk for the development of various
pathologies including cardiovascular disease. To study this, we derived cardiomyocytes
(CMs) from human-induced pluripotent stem cells and exposed them to simulated
microgravity (SMG).

February 13-16, 2024 195
Poster Session A: Commercial

001 Jeffrey Smith
(1646093) DEEP SPACE LOGISTICS CAPABILITIES AND OPPORTUNITIES TO
SUPPORT FUTURE HUMAN RESEARCH AT THE GATEWAY
NASA's Deep Space Logistics missions will deliver cargo to the Gateway, in orbit around the
Moon, and provide a flexible volume and workspace in deep space to augment Gateway
capabilities for astronaut crews perform the critical human research in deep space that will
extend human exploration from the Moon, on to Mars.
002 Alain Berinstain; Gentry Barnett; Twyman Clements; Shelby Giza; Danielle Rosales
(1646645) R&D AND IN-SPACE PRODUCTION APPLICATIONS: AUTOMATED
CAPABILITIES IN MICROGRAVITY FOR THE NEXT DECADE AND BEYOND
Prioritizing the development of automated technology counters the intimidation of an initially
complex and expensive process, making it possible to build both an infrastructure and a
reliable future for iterative, long-term science and manufacturing applications in microgravity.
003 Dr Jon Sen; Mohammed Ahmed; Christopher Mason; Yvette Gonzalez; Andrew Lee;
Shawna Pandya; Aaron Persad; Bader Shirah
(1649291) THE APPLICATION OF QUANTITATIVE PUPILLOMETRY FOR
NEUROLOGICAL MONITORING IN SHORT DURATION SPACEFLIGHT
Novel automated pupillometry provides rapid quantification of various pupillary parameters
which reflect the structural and functional integrity of wide ranges of intracranial networks
involved in the genesis of pupillary responses. This study investigates the first use of
automated pupillometry in space as a novel neuro-monitoring tool, on the Axiom-2 mission to
the International Space Station

February 13-16, 2024 205
Poster Session A: Data Management

004 Judith Peace; Adrienne Hoyt; Kathryn Richards
(1612897) THE HUMAN RESEARCH PROGRAM DATA MANAGEMENT & GRANT
LIFECYCLE INFOGRAPHIC AND TRAINING SHEETS
Due to the size and overlap of information in the HRP Data Management Plan (DMP) and the
NASA & Cooperative Agreements (GCAM), members from the HRP workforce requested
“cheat sheets” containing bite size pieces of information from some of the basic topics and
recurring challenges during the active research and closeout phases.
005 Judith Peace; Mary Kirby; Vanessa Lehman
(1616891) THE HUMAN RESEARCH PROGRAM GRANT LIFECYCLE & DATA
INTEGRATION SCHEDULE: INFOGRAPHIC
The Human Research Program (HRP) Grant Lifecycle infographic poster tells a short story
about a federally awarded grant with emphasis on the HRP Data Integration Schedule and is
intended to help the HRP workforce understand the timeline for this research procurement
and where their specific tasks fall within the 4 phases of the grant lifecycle.
006 Hamed Valizadegan; Michael Von Pohle; Adwait Sahasrabhojanee; Janani Iyer; Sandeep
Shetye; Dan Berrios; Adrienne Hoyt; Truong Le
(1629954) LSKNOWLEDGE: NEXUS FOR TRANSFORMATIVE SCIENTIFIC
DISCOVERIES AND ENHANCED INFORMATION RETRIEVAL IN NASA LIFE SCIENCES
PORTAL
We have launched LSKnowledge, aimed at enhancing the information retrieval capabilities of
NLSP. Our primary goal is to develop a robust semantic search system. This system will
empower HRP (Human Research Program) researchers to navigate NLSP data repositories
more efficiently and precisely, catalyzing the process of hypothesis formation and scientific
breakthroughs.
007 Robert Beaton; Brenna Wheeler; Sara Jorgensen; Jacqueline Charvat; Devan Petersen;
Jessica Keune
(1637953) REDISCOVERING APOLLO BIOMEDICAL DATA TO SUPPORT ARTEMIS: THE
APOLLO RECORDS SYNTHESIS PROJECT
As a joint effort between the Life Sciences Data Archive (LSDA) and the Lifetime
Surveillance of Astronaut Health (LSAH), the Apollo Records Synthesis Project (ARSP)
seeks to expand the available biomedical knowledge base for the Apollo missions and to
develop datasets for buying down risk on the Artemis Program by examining physical
records located across a wide range of collections.
008 Christina Johnson; Richard Barker; Afshin Beheshti; Daniela Bezdan; Gilbert Cauthorn;
Melanie Correll; Sylvain Costes; Rachel Gilbert; Stephen Lantin; Danielle Lopez; Rafael
Loureiro; Gbolaga Olanrewaju; Xavier-Lewis Palmer; Kristen Peach; Sigrid Reinsch; Nitin
Singh; Nathaniel Szewczyk; Chad Vanden Boscht; Sarah Wyatt; Paola Castaño
(1647304) TRACKING COMMUNITY BUILDING IN OPEN SCIENCE
Here, we present greater detail about the makeup and participation metrics of the various
Analysis Working Groups affiliated with the NASA Open Science Data Repository and details
of successful peer-reviewed publication campaigns.

February 13-16, 2024 215
009 Amanda Saravia-Butler; Lauren Sanders; Ryan Scott; Danielle Lopez; Samrawit Gebre;
Sylvain Costes
(1647859) ENABLING MODEL ORGANISM AND COMMERCIAL ASTRONAUT DATA
ACCESS THROUGH THE NASA OPEN SCIENCE DATA REPOSITORY
Here we describe the robust privacy and security protocols implemented by the Open
Science Data Repository (OSDR) to safeguard sensitive health data from astronauts while
facilitating metadata and processed data sharing for research purposes. We further provide a
road map for navigating the vast amount of data and associated metadata provided for each
Inspiration 4 study hosted on OSDR.
010 Eliah Overbey; JangKeun Kim; Christopher Mason; Jaime Mateus; Marissa Rosenberg;
Krista Ryon; Bader Shirah; Braden Tierney; Cem Meydan; Jeremy Wain Hirshberg
(1649477) THE CORNELL AEROSPACE MEDICINE BIOBANK (CAMBANK) AND THE
SPACE OMICS AND MEDICAL ATLAS (SOMA)
The Cornell Aerospace Medicine Biobank (CAMbank) has been created to store and
disseminate spaceflight samples to the scientific community and the Space Omics and
Medical Atlas (SOMA) will enable public data visualization of spaceflight biomarker and
omics data.

225
Poster Session A: Exploration Medical Capability (ExMC)

093 Avinav Sahoo
(1609330) R2-D2 ASTRO-MED: ARTIFICIAL INTELLIGENCE GENERATED MEDICAL
HISTORY TAKING CHATBOT FOR SPACE MEDICINE.
This is chatbot user interface to take medical history of astronauts who are having inflight
medical conditions or emergencies. This chatbot will collect data and transmit it to the ground
control system for doctors to analyse the conditions and plan the management accordingly .It
is named R2-D2 Astro-Med after the Star Wars character R2-D2.
094 Nicholas Antonino; Brent Feldt; Sarah Maginnis; George Pantalos; Robert Rigor
(1630997) APPENDECTOMY USING A HERMETIC SURGICAL SYSTEM (HESS): A
PROTOTYPE FOR SURGERY IN SPACE AND AUSTERE ENVIRONMENTS
Endoscopic surgery using a Hermetic Surgical System (HeSS) as a prototype for surgery in
space and austere environments.
095 Sheyna Gifford; Rose Jardine; Tyler Pugeda; Luke Brane; Erik Viirre
(1631772) MEDICAL OPERATIONS CONSIDERATIONS FOR ASTRONAUTS WITH
DISABILITIES
This observational study explores the development of protocols informing medical
operational considerations for people with disabilities and shows that, with carefully
conducted health and safety reviews, and in some cases with individualized flight plans,
researchers with disabilities can participate safely in, and successfully conduct research
during, altered gravity parabolic flights.
096 Philippe Arbeille
(1633210) LIVER TISSUE CHANGES DURING 6-MONTH SPACE FLIGHT MEASURED BY
ULTRASOUND RF SIGNAL PROCESSING.
The objective of the present research was to check using the ultrasound RF signal
processing if the Liver tissue response to ultrasound changed during the spaceflight.
097 Clara Gasiewski; Lauren McIntyre; Drayton Munster; Jerry Myers; Sean Bostic
(1637542) NOVEL APPROACH TO SIMULATING DIAGNOSTIC CAPABILITIES IN
MEDICAL RESOURCE RISK ASSESSMENT
This analysis presents an alternative means of representing diagnostic resources within the
medical risk domain for spaceflight through simulating the effect in risk, resource
consumption, and competition for resources of a missing or depleted diagnostic.
098 Drayton Munster; Sean Bostic
(1637668) ENABLING RAPID AND REPRODUCIBLE MODELING VIA SOFTWARE
DEVELOPMENT BEST PRACTICES
Adopting best practices from software engineering enables research that is reproducible and
sharable with full provenance in the code and data.
099 Sean Bostic; Drayton Munster
(1637676) WEB-BASED APPLICATION FOR THE DISTRIBUTION OF COMPUTATIONAL
ANALYSIS RESULTS
A web application was created to allow the information in an analysis result to be distributed
in a more streamlined, interactive, and accessible manner.
100 Sylwia Kaduk; Janina Post; Gabriel Huber; Lara Dietlein; Carla Tamai; Raphael Kneffel;
David Cyrol; Stefan Sammito
(1638312) LESSONS FOR ULTRASOUND USE IN SPACE- FAST ULTRASOUND
SCANNING BY MEDICALLY INEXPERIENCED INDIVIDUALS
A study investigating the usability, feasibility, and quality of conducting FAST ultrasound
scans by medically inexperienced individuals relying solely on the in-built instructions of
TEMPUS PRO vital signs monitor.
February 13-16, 2024 235
101 Julia Scarpa; Gary Strangman

(1641955) THE PERIOPERATIVE PERIOD: AN UNDERAPPRECIATED TESTING
GROUND FOR AEROSPACE MEDICAL TECHNOLOGY
Collaboration with perioperative practitioners to test aerospace medical technology can
provide gold-standard validation across a broader physiologic spectrum and in a more
accessible, cheaper, rapid fashion than traditional pipelines.
102 Allison Porter; Aleksandra Stankovic; Katya Arquilla
(1643590) NATURALISTIC APPROACH TO CAPTURING KEY SITUATION AWARENESS
CUES IN EMERGENCY POINT-OF-CARE ULTRASOUND FOR USE IN EARTH-
INDEPENDENT SURFACE OPERATIONS
This paper discusses the process of identifying situation awareness cues via direct
observations of urgent medical care in a well-equipped hospital setting (Emergency
Department), findings from those observations, and the characterization of differences
between the wilderness medicine and hospital settings to begin bridging this work to the
surface exploration paradigm.
103 Brita Mittal; Amran Asadi; Marissa Rosenberg
(1643687) ASSESSMENT OF ACUTE UPPER AIRWAY CHANGES DURING
SPACEFLIGHT USING ENDOSCOPIC AIRWAY EXAMINATION AND ULTRASOUND
This prospective, observational pilot study aims to characterize changes to human upper
airway anatomy induced by sustained microgravity exposure using a novel combination of
transnasal endoscopic airway examination and ultrasound performed during a commercial
spaceflight mission.
104 Kendall Farnham; Ryan Halter; Ethan Murphy
(1650622) INTEGRATED ULTRASOUND AND ELECTRICAL IMPEDANCE TOMOGRAPHY
FOR IMPROVED KIDNEY STONE DETECTION
We are integrating ultrasound and EIT to increase sensitivity to kidney stones with high-
contrast, dual-modality imaging for improving treatment outcomes of astronauts on deep
space and long-duration missions.
105 Alamelu Sundaresan; Vivek Mann
(1646463) ENVIRONMENTAL DUST EXPOSURE - LUNAR DUST AND THE HUMAN
CHALLENGE FOR MOON MISSIONS
This study will explore and explain the effects of lunar simulant on skin fibroblasts, immune
cells and lung epithelial cells. Preliminary results show evidence of mutagenesis in the lung
cells, increase in inflammatory mediators in immune cells and dysregulation of focal
adhesion kinases in the fibroblasts.
106 Luke Apisa; Yann Wendu-Foyet; Tovy Haber Kamine; Erik Antonsen; Lauren McIntyre; Jerry
Meyers; Dana Levin
(1647238) USE OF PREDICTIVE ANALYTICS TO DETERMINE THE NECESSARY TIME
HORIZON OF SURGICAL CAPABILITIES FOR SPACE MISSIONS
To begin to bound the problems posed by specialty-specific causes of return to definitive
care, this study implemented MEDPRAT in conjunction with the IMPACT Evidence Library to
estimate the number of person years before the risk of surgical pathology might justify the
need for on-board surgical capabilities in long-duration human spaceflight.
107 Megan Waldock; Carly O'Rourke; Alex Roberts
(1647618) LONG TERM HEALTH METRICS - YET2 EXPERT SEARCH
Beginning July 2023 and expected to complete in November 2023, yet2, a technology
scouting and open innovation consulting company, is working with NASA HRP to identify
experts to critically evaluate existing long-term health (LTH) metrics and provide guidance on
their applicability to astronauts.

February 13-16, 2024 245
108 Megan Waldock; Roddy MacCallum; Carly O'Rourke

(1647646) CLINICAL DECISION SUPPORT TECHNOLOGY - YET2 TECHNICAL SURVEY
From September 2023 to November 2023, yet2, a technology scouting and open innovation
consulting company, worked with NASA XMIPT to conduct a landscape analysis of
partners/technologies for diagnosis, monitoring, and treatment of medical conditions on
future deep-space missions.
109 Ryan Lacinski; Jonathan Steller; Arian Anderson; Dana Levin; Ariana Nelson
(1647670) HARNESSING ARTIFICIAL INTELLIGENCE FOR MEDICAL DIAGNOSIS AND
TREATMENT DURING SPACE EXPLORATION MISSIONS
The purpose of this study was to identify Artificial Intelligence (AI) tools currently available or
in development for the assistive diagnosis and care of medical conditions predicted for an
extended duration Lunar mission.
110 Ariana Nelson; Tovy Kamine; Jonathan Steller; Arian Anderson; Dana Levin
(1647678) EXTRATERRESTRIAL PRE-HOSPITAL ORBITAL NETWORK EVACUATION
Examination of five potential methods for medical evacuation from orbital habitats: 1)
Ground-launched ambulance and medical crew (emergency medical services [EMS] model),
2) Prepositioned lifeboat (ISS model), 3) Orbital ambulance (U.S. Coast Guard model), 4)
Ground launch lifeboat (near-peer conflict evacuation model), 5) Space based hospital (U.S.
Navy model).
111 Craig Kutz; Amit Mistry; Charles Dukes
(1647806) KETAMINE - AN ALTERNATIVE FOR ACUTE SUICIDALITY IN SPACEFLIGHT
The goal of this study was to review current literature and collate the understanding of
ketamine as a safe, effective pharmacological adjunct for acute suicidality in spaceflight.
112 Aristée Thevenon; Philippe Arbeille; Didier Chaput; Orphée Faucoz
(1648600) ECHOFINDER: EVALUATION OF AN EARTH-INDEPENDANT ULTRASOUND
ACQUISITION PROTOCOL ON THE INTERNATIONAL SPACE STATION
To allow earth-independent ultrasound imaging sessions, we propose an innovative solution
based on augmented reality (AR) and artificial intelligence (AI) which will be tested during
Axiom 3 mission.
113 Katie Harris; David Lerner; Michael Pohlen
(1648913) UTILITY OF WHOLE BODY MAGNETIC RESONANCE IMAGING FOR PRE-
FLIGHT MEDICAL MISSION RISK REDUCTION
Whole body magnetic resonance imaging pre-flight may be useful to buy down mission risk
for approximately 10% of the conditions on the ExMC ICL, and could contribute greatly to
spaceflight physiology research when combined with post-flight studies.
114 Abhilash Chandra; Esther Ko; Christopher Markovic; Edward Palmar; Steve Cook
(1649285) THE ROLE OF IMMERSIVE TECHNOLOGIES FOR HUMAN FACTOR
OPTIMISATION AND PREPARATION FOR EXTRA-TERRESTRIAL MEDICAL AND
SURGICAL EMERGENCIES.
This abstract demonstrates how SABRN is working towards utilising VR technologies to
prepare non-clinical personnel to perform clinical procedures prior to deployment to hostile
environments, and then using AR technologies to support these non-clinical personnel
perform clinical procedures after deployment to hostile environments.
115 Shawna Pandya; Mike Wesolowski
(1650042) MIXED REALITY, COLLABORATIVE POINT-OF-CARE ULTRASOUND FOR
AUSTERE AND RESOURCE LIMITED ENVIRONMENTS
In this study, we review our experiences with testing and deploying a mixed-reality,
collaborative, point-of-care SiEVRt ultrasound system developed to address gaps in care in
both rural and remote environments, as well as in exploration-class missions.to aid with
diagnosis and clinical decision-making.

February 13-16, 2024 255
116 Michael Marge; Tina Beard

(1650073) IAASS/AEROSPACE CORPORATION HUMAN RESEARCH PROGRAM FOR
CIVILIANS IN SPACEFLIGHT AND SPACE HABITATION
The International Association for the Advancement of Space Safety/the Aerospace
Corporation co-sponsored the development of a Human Research Program for Civilians in
Space Travel and Space Habitation that identifies the types of data collection and scientific
research required to make space accessible, healthy, and safe for everyone, even for those
with chronic health problems and disabilities.
117 Kenny Chao; Atandra Burman; Jitto Tittus; W. Frank Peacock; Amit Padaki; Dana Levin
(1650315) NON-INVASIVE PROTEIN MEASUREMENT BY TRANSCUTANEOUS SENSOR
Remote Cardiac Enablement (RCE) has developed a transcutaneous sensor capable of
detecting cardiac biomarkers such as troponin as a proof-of-concept of reusable, point-of-
care sensing technology that allows for the potential detection of innumerable proteins -
providing an expanded scope of diagnostic testing for spaceflight while reducing the mass,
volume, and power footprint.
118 Dora Babocs; Matthew Melin; Gustavo Oderich; Rowena Christiansen
(1650598) SURGICAL CONCERNS FROM ACCELERATED ATHEROSCLEROSIS
DURING LONG-DURATION SPACEFLIGHT
Many studies have investigated cardiovascular aspects, fluid shifts, space radiation effects
on crew health, and vascular surgical aspects in space; however, a notable gap exists with
no dedicated research on atherosclerosis from a surgeon's perspective, making
endovascular surgery an enticing prospect.
119 Dora Babocs; Gustavo Oderich; Matthew Melin; Rowena Christiansen
(1643994) TRADITIONAL SURGICAL METHODS AND CUTTING-EDGE TECHNIQUES:
PIONEERING ENDOVASCULAR SURGERY IN LONG-DURATION HUMAN SPACE
EXPLORATION
As we extend our reach beyond our planet, understanding the implications of space travel on
human health and developing appropriate medical responses becomes paramount. The
synergy between traditional surgical methods and cutting-edge techniques could shape the
future of space medicine, ensuring the health and well-being of astronauts as they journey
into the cosmos.
120 Dora Babocs; Matthew Melin; Gustavo Oderich; Rowena Christiansen
(1650650) FROM A SURGEON'S PERSPECTIVE: FACTORS INFLUENCING WOUND
HEALING DURING LONG-DURATION HUMAN DEEP SPACE EXPLORATION
Understanding challenges like potential wound healing delays and the effects of microgravity
on surgical outcomes is pivotal for successful long-duration space missions, necessitating
advancements in surgical techniques, wound care strategies, and preventative measures to
ensure astronauts' health and safety as humanity explores deeper into space.
161 Donghyeon Ryu
(1652586) PHYSICAL DIGITAL TWIN BUILT USING IN-PLANE STRAINS ON BODY
SURFACE FOR HEALTH MONITORING DURING A LONG-TERM SPACE TRAVEL
This presentation is to propose a novel way to create a physical digital twin using an in-plane
strain on human body surface for health monitoring in space.

265
Poster Session A: Extravehicular Activity (EVA)

172 Katherine Maguire; John Caruso; Jennifer Daily; Neel Patel; Margaret Wydotis
(1622915) CONTINUOUS PALM COOLING ON HEART RATE AND AUDITORY CANAL
TEMPERATURE VALUES
The abstract describes palm cooling's ability to reduce heat rate and auditory canal
temperatures.
173 Neel Patel; John Caruso; Jennifer Daily; Frances Maguire; Pete Quesada; Margaret Wydotis
(1624020) CONTINUOUS PALM COOLING'S EFFECT ON HEAT TRANSFER AND HAND
TEMPERATURES
The abstract describes the cooling effects of palm cooling on palmar skin temperatures and
heat transfer across the palm.
174 Maddie Haas; Kristin Moeller; Pascaline Tausend; Jack Calhoun; Darren Hartl
(1648716) DESIGN AND OPTIMIZATION OF LIQUID COOLING AND VENTILATION
GARMENT PERFORMANCE VIA THERMAL FINITE ELEMENT ANALYSIS
This research describes a thermal finite element analysis model of a cooling tube layout of a
LCVG developed using the Abaqus Unified FEA suite, focused on maximizing metabolic heat
removal by defining the layout of cooling tubes within the garment through topological
optimization.
175 Derek Nusbaum; Andrew Abercromby; Tony Babb; Mathias Basner; Alexander Baughman;
Benjamin Levine; James Pawelczyk; Robert Scully
(1652313) EFFECT OF CARBON DIOXIDE EXPOSURE ON PHYSICAL AND COGNITIVE
PERFORMANCE IN A SIMULATED SPACEFLIGHT CONTINGENCY SCENARIO
This study will provide valuable information regarding how various partial pressures of CO2
exposure impact acute health, as well as cognitive and physical performance during
simulated contingency lunar EVA.

February 13-16, 2024 275
Poster Session A: Food

035 Jess Bunchek; Mary Hummerick; LaShelle Spencer; Matthew Romeyn; Millennia Young;
Robert Morrow; Cary Mitchell; Grace Douglas; Raymond Wheeler; Gioia Massa
(1644672) PICK-AND-EAT SPACE CROP PRODUCTION FLIGHT TESTING ON THE
INTERNATIONAL SPACE STATION
The VEG-04 study with Mizuna mustard has found that yield, organoleptic acceptability,
microbial load and food safety, nutritional content, and the resources required to grow fresh
produce in the Veggie vegetable production chamber on the ISS can be influenced by the
selected lighting recipe, duration and method of cultivation and harvesting, and microgravity
environment.
036 Alain Maillet; Davis Moreeuw; Jean-Loup Cartier; Florence Clement; Thierry Varlet; Marc
Della-Siega; Philippe Chancerel; Elisabeth Araujo; Marie Chancerel; Tristan Hermel; Florian
Roselli; Anne-Dominique Malinge; Nicolas Guignard; Remi Canton
(1648379) FOOD PROCESSOR: A FIRST RECIPE COOKED ON BOARD THE ISS
We would like to present this abstract as a poster, it will describe the Food Processor project
(CNES) and the first recipe cooked on board the space station
037 Grace Douglas; Holly Dlouhy; Audrie Colorado; Douglass Diak; Norberto Gonzalez-Juarbe;
Cody Gutierrez; Satish Mehta; Sara Whiting; Hernan Lorenzi; Brian Crucian; Scott Smith;
Millennia Young; Sara Zwart
(1652468) THE INTEGRATED IMPACT OF DIET ON HUMAN IMMUNE RESPONSE,
THE GUT MICROBIOTA, AND NUTRITIONAL STATUS DURING ADAPTATION TO
SPACEFLIGHT
We expect this study to provide evidence of beneficial impact of this enhanced diet on crew
health and adaptation to spaceflight. These data will aid in evidence-based mass-risk trades
for food system design and development of targeted dietary interventions for future
exploration-class space missions.
038 Michael Dzakovich
(1652494) NOTHING TO BE SALTY ABOUT: MODEST SALINITY STRESS IMPROVES
CAROTENOID BIOACCESSIBILITY FROM SPINACH
Mild salinity stress can improve delivery potential of fat-soluble phytochemicals from spinach
without negatively impacting yield.

February 13-16, 2024 285
Poster Session A: Immune

021 Cassian Yee; Paul Hung; Jamie Lin; Shialbala Singh
(1634730) THE EFFECT OF MICROGRAVITY ON HUMAN T CELL DIFFERENTIATION
AND MEMORY
T cells require a well-orchestrated series of biochemical, structural and kinetic events for
activation and differentiation into functional memory T cells. Understanding the effects of
microgravity on these pathways can modulate T cell memory and exhaustion-resistance can
have broad and immediate implications for immunosurveillance, vaccine, and T cell therapies
for cancer and other diseases
022 Maria Moreno-Villanueva; Stephanie Krieger; Ye Zhang; Brian Crucian; Honglu Wu
(1644824) GENE EXPRESSION OF PERIPHERAL BLOOD MONONUCLEAR CELLS OF
CREW MEMBERS DURING LONG-DURATION SPACE MISSIONS INDICATE
DYSREGULATION OF IMMUNOLOGICAL AND CELL SURVIVAL MECHANISMS
PCR analysis were performed in peripheral mononuclear cells from the ISS crew members.
A list of 62 genes were carefully selected addressing immunological and cell survival
pathways. Differentially expressed genes indicated changes in chemokine receptor activity,
chemokine binding, toll-like receptors, adhesion molecules and cellular response to DNA
damage.
023 Bikash Konda; Minon Ishizuka; Hasan Nisar; Jessica Kronenberg; Claudia Schmitz;
Sebastian Diegeler; Edwin Mulder; Maria Bohmeier; Irmtrud Schrage-Knoll; Jens Jordan;
Christine Hellweg
(1646059) INFLUENCE OF HEAD-DOWN TILT BEDREST ON DNA REPAIR CAPACITY
Physical deconditioning by head-down tilt bedrest did not alter DNA repair capacity in
peripheral blood mononuclear cells which was investigated by immunofluorescence staining
of gamma H2AX quantified by flow cytometry.
024 Nathalie Pineda
(1647588) CONCURRENT EXPOSURE TO CHRONIC IRRADIATION AND/OR
SIMULATED MICROGRAVITY ALTERS SPLENIC IMMUNE CELLS PHENOTYPE AND
FUNCTION
To enhance mission safety, female C57BL/6 mice were subjected to chronic γ-irradiation
(CIR) and hind-limb unloading (HLU) for 29 days, revealing that the combined exposure to
microgravity and CIR significantly disrupted immune cell properties, CD4+/CD8+ and B cell
functions, and cellular structure, underscoring the importance of developing
countermeasures for deep-space missions.
025 Honglu Wu; Brian Crucian; Stephanie Krieger; Maria Moreno-Villanueva; Nathaniel Stucky;
Ye Zhang
(1649410) ROLE OF PIEZO1 IN T CELL ACTIVATION UNDER SIMULATED
MICROGRAVITY
Piezo1 is a known mechanosensing gene and has been shown to be critically involved in
human T cell activation. The role of the Piezo1 gene in T cell activation under simulated
microgravity was investigated.
026 Patrick Rydzak; Christian Castro; Hang Nguyen; Audrey Almengor; Sarah Stahl-Rommel;
Satish Mehta; Douglass Diak; Miten Jain; Stephen Tyring; Brian Crucian; Sarah Castro-
Wallace
(1649732) SPACEFLIGHT-COMPATIBLE METHOD FOR THE IN-SITU, REAL-TIME
DETECTION OF HERPESVIRUS REACTIVATION IN OVERWINTER CREW AT PALMER
STATION USING NANOPORE SEQUENCING
Optimized previously developed method for the detection of herpesviruses from saliva to
allow the method to be spaceflight compatible.

February 13-16, 2024 295
027 Daniel Tavakol; Ivana Matkovic; Danielle Mendonca; Gerarda Cappuccio; Keith Yeager;
Ilaria Baldassarri; Francois Chesnais; Roberta Lock; Diogo Teles; Connie Chen; Pamela
Graney; Griffin Daly; Mirjana Maletic-Savatic; Gordana Vunjak-Novakovic
(1650071) EXTENDING THE LIFETIME OF SYSTEMIC, MULTI-ORGAN BIOENGINEERED
HUMAN TISSUE MODELS WITH INTEGRATED IMMUNE COMPONENTS
In this work, we are developing bioengineering platforms of the heart-bone marrow-
vasculature (at Columbia University) and brain-bone marrow-vasculature tissues (at Baylor
College of Medicine), to enable immune cell-tissue interactions over long durations of culture
(up to 6 months).
028 Dagan Loisel; Mackenzie Costello; Brian Crucian; Colby Fane-Cushing; Elizabeth Marini;
Jordyn Morey; Mayra Nelman-Gonzalez; Madeline Van Winkle; Melissa VanderKaay
Tomasulo
(1650311) VR MEDITATION COUNTERMEASURE HAS POSITIVE EFFECTS ON
AUTONOMIC, MOOD, AND IMMUNE INDICATORS OF THE STRESS RESPONSE IN A
LAB SETTING
Our results indicate that VR-delivered guided meditation was associated with a decrease in
systolic blood pressure and negative affect, an increase in positive affect, and positive
changes in salivary biomarkers of stress.
029 Christopher Mason
(1650592) SINGLE-CELL MULTI-OME AND IMMUNE PROFILES OF THE INSPIRATION4
CREW REVEAL CELL-TYPE, SEX, AND MICROBIOME-SPECIFIC RESPONSES AND
RECOVERY
We report here findings on the SpaceX Inspiration4 (i4) mission, an all civilian-crewed
commercial orbital spaceflight, including multi-omic, in-depth immune system profiling at the
single-cell level for the four-member crew with a broad age range (29-50 years old at launch
date) and biomedical background.
030 Mayra Nelman-Gonzalez; Forrest Baker; Alexander Chouker; Brian Crucian; Douglass Diak;
Cody Gutierrez; Grace McKenzie; Satish Mehta; Rickie Simpson; Scott Smith; Sara Zwart
(1652100) DRY SALIVA DEVELOPMENT - ARTEMIS
Deployment of prolonged deep space missions carry increased crew health risks. Transition
to Gateway and Artemis will limit the available up mass and biosample return capability
resulting in challenges to monitor crew health. The goal of this study is the development of
dried biosampling as a technology that enables sample collection and tracking of crew health
during exploration class missions.
031 Douglass Diak
(1652228) HERPES VIRAL SHEDDING DECREASES IN ANTARCTIC EXPEDITIONERS
WITH PROPHYLACTIC ANTIVIRAL (VALACYCLOVIR) TREATMENT: A CLOSER LOOK
AT IMMUNE STRESS
This study will provide an analysis of additional salivary inflammatory and immune
biomarkers to further solidify the claim that Antarctic expeditioners under prophylactic
antiviral treatment experience not only less viral stress, but also reduced inflammation and
immune stress than those taking a placebo.
032 Cody Gutierrez; Alexander Chouker; Brian Crucian; Douglass Diak; Dagan Loisel; Satish
Mehta; Mayra Nelman-Gonzalez; Sergey Ponomarev; Rickie Simpson; Scott Smith;
Raymond Stowe; Melissa Tomasulo; Sara Wallace; Sara Zwart
(1652232) VALIDATION OF MULTISYSTEM COUNTERMEASURES PROTOCOL FOR
SPACEFLIGHT DURING ANTARCTICA WINTER-OVER AT PALMER STATION (PALMER
COUNTERMEASURES)
Countermeasure development and validation at Plamer Station in Antartica.

305
033 Sara Bustos Lopez; Douglass Diak

(1652305) INFLUENCE OF MICROGRAVITY ON BACTERIAL PATHOGENS VIRULENCE
AND IMMUNE CELL FUNCTION RELEVANCE FOR INFECTIOUS DISEASE RISK DURING
SPACE FLIGHT
This study examines the impact of the pathogens Pseudomonas aeruginosa, Salmonella
enterica, and Burkholderia cepacia pre-exposed to microgravity or ground based simulated
microgravity on human PBMCs recovered from astronauts and ground based control
subjects. Data shows an altered impact of microgravity on immune cell activation, cytokine
profile, and immune cell behavior by imaging analysis.
034 Elizabeth Beattie; Kyle Smith; Grace McKenzie; Brian Crucian; Christopher Connaboy;
Emmanuel Katsanis; Richard J. Simpson
(1673128) ASSOCIATIONS BETWEEN VOLUNTARY EXERCISE TRAINING, STRESS
BIOMARKERS AND IMMUNE DYSREGULATION DURING A 45-DAY HUMAN
EXPLORATION RESEARCH ANALOG (HERA) MISSION
ASSOCIATIONS BETWEEN VOLUNTARY EXERCISE TRAINING, STRESS BIOMARKERS
AND IMMUNE DYSREGULATION DURING A 45-DAY HUMAN EXPLORATION
RESEARCH ANALOG (HERA) MISSION

315
Poster Session A: MicroHost

016 Kristyn Hoffman; Adriana Babiak-Vazquez; Mark Ott
(1643722) HISTORICAL MICROBIAL POPULATION OF THE ISS AND ITS CLINICAL
RELEVANCE
The historical microbial population was analyzed with clinical symptom incidence from crew
health records with random forest machine learning assessment to uncover potential host
pathogen relationships on ISS.
017 Ralf Moeller; Alena Warkentin; Laura de Boni; Petra Frings-Meuthen; Katharina Siems;
Christine Hellweg
(1646252) GUT MICROBIOME DYNAMICS UNDER SIMULATED MICROGRAVITY - FIRST
INFORMATION FROM THE SPACEFLIGHT-ASSOCIATED NEURO-OCULAR SYNDROME
(SANS) -COUNTERMEASURES STUDIES
In the area of space exploration, numerous factors are able to decrease the quality of health
and recovery of space travelers. Among those, weightlessness comes with significant
challenges. Besides the negative effect it has on bone density, muscle mass, and risk for
space-associated neuro-ocular syndrome, it also has the ability to influence the composition
of the gut microbiome.
018 Frances Donovan; Natalie Ball; Sadie Downing; Ami Hannon; Hiromi Kagawa; Jing Li; Hami
Ray; Oscar Roque; Andrew Settles; Kevin Sims; Sandra Vu
(1649533) STRATEGIES FOR DETERMINING SAFETY OF FERMENTED FOODS
PRODUCED IN SPACE
An evaluation and comparison of various pathogen detection methods and the challenges for
use with high microbial content products.
019 Mark Ott; Jennifer Barrila; Sandhya Gangaraju; Laura Banken; Jiseon Yang; Richard Davis;
Audrie Medina-Colorado; Ji Sun Park; Eleanor Blakely; Phillip Stafford; Cheryl Nickerson
(1649639) EFFECTS OF LOW DOSE RADIATION AND RADIATION
COUNTERMEASURES ON INFECTION BY SPACEFLIGHT ANALOGUE CULTURED
SALMONELLA USING 3-D BIOMIMETIC HUMAN TISSUE MODELS
This study investigates whether the already enhanced infection potential of spaceflight
analogue cultured S. Typhimurium will be further exacerbated when used to infect human
cells exposed to low dose radiation and if this enhanced pathogenicity can be mitigated by a
radioprotective compound.
020 Polina Shtern; Charlotte Pouwels; Margot Issertine; Gauravam Majmudar; Rowan Moorkens
O’Reilly; Alexandra Lissouba
(1650155) UV LIGHT FOR SPACE LAUNDRY: MITIGATING MICROBIAL RISKS ON
LONG-DURATION CREWED MARS MISSIONS
This research project aims to mitigate the impact of long-duration spaceflight on the human
skin microbiome by testing the effectiveness of a portable UV-C device on astronaut clothing
during an analog mission, with the goal of reducing microbial counts, minimizing infection
and allergic response risks, and supporting a balanced skin microbiome for long-term Mars
missions.

February 13-16, 2024 325
Poster Session A: Mixed Topics

351 Steven Rader
(1610070) OPEN INNOVATION SUCCESS STORIES IN HEALTH RELATED
GOVERNMENT RESEARCH
This presentation will highlight a number of successful open innovation projects run through
the NASA Tournament Lab for NASA, CDC, HHS, NIH, NSF, and VA to demonstrate the
power of open innovation (available to IWS attendees via NASA CoECI) to enhance and
accelerate health related research.
352 Helen Schell
(1622823) THE HUMAN SPACESHIP, UPON THE MOON
The Human Spaceship: New 21st century space endeavors have resulted in the need for
extensive research into altered gravity’s impact on visual physiology and perception, so how
do artists represent the need for creative thinking, which transcends academic barriers,
promoting cross-disciplinary skills to achieve long-term human spaceflight goals and living,
(Moon, Mars and space stations)?
353 Sandeep Kamat; Ivan D'souza
(1641789) PUBLICATION TRENDS ANALYSIS: A NEW PERSPECTIVE ON ASSESSING
RESEARCH OUTPUT
We present a new approach to assessing research output through a quantitative analysis of
volume of peer-reviewed journal articles indexed in the NASA Task Book bibliography, which
will help monitor overall publication activity and identify publication trends for specific
subsystems related to countermeasures against spaceflight hazards.
354 Vanessa Farsadaki
(1642159) SPACE PREGNANCY BIOETHICS
Exploring ethics of space pregnancy: Risks, benefits, and societal impact.
355 Vanessa Farsadaki
(1642174) THE DEMOCRATIZATION OF SPACE MEDICINE
Democratizing space medicine is imperative as space travel becomes more accessible,
necessitating education, innovation, and collaboration for comprehensive healthcare
provision beyond Earth.
356 Alyssa Adcock; Allison Caherty; Jennifer Buss
(1643066) WHY IT MATTERS: STRATEGICALLY COMMUNICATING YOUR SCIENCE
To support both HRP's mission to mitigate the risks of human spaceflight and strategic goal
to communicate impact, the Potomac Institute for Policy Studies presents a science
communication framework to help communicate science more effectively to relevant
stakeholder groups across academia, industry, and government, including policy makers.
357 Meena M. C. Shekar; John Hansen
(1649466) THE UNSUNG HEROES OF APOLLO 11: ANALYZING MISSION-CRITICAL
CONVERSATIONS THROUGH SPEAKER DIARIZATION ON FEARLESS STEPS APOLLO
we implement a novel framework based on dynamic Graph Attention Network for speaker
diarization and clustering. Further, we also identify, track, analyze key speakers of interest
throughout the complete Apollo 11 mission. One of the first initiatives to to identify and
extract meta-labels for speakers serving as a lasting tribute to the unsung heroes of the
Apollo mission.

February 13-16, 2024 335
358 Aditya Joglekar; Iván López-Espejo; John H.L. Hansen

(1650171) FEARLESS STEPS APOLLO: IDENTIFYING CONVERSATIONAL MISSION-
CRITICAL TOPICS IN NASA APOLLO MISSIONS AUDIO BASED ON KEYWORD
SPOTTING
In this study, we focus on customizing keyword spotting (KWS) and topic detection
mechanisms, laying the foundational groundwork for advanced conversational
comprehension.
359 Chloe Tuck; Ursula Koniges; Wanessa Priesmeyer
(1650180) LEVERAGING SUBORBITAL FLIGHT TESTING TO ADVANCE SCIENCE AND
TECHNOLOGY IN THE EXPANDING SPACE ECONOMY
Presenters from two NASA programs – Flight Opportunities and the Biological and Physical
Science (BPS) Division’s Commercially Enabled Rapid Space Science (CERISS) initiative –
will discuss ways of accessing flight tests to advance technologies that meet U.S. space
exploration priorities and support the expanding space economy, including those that support
sustained human presence in space.
360 Xi Liu; John Hansen
(1650663) A CEPSTRUM AND SPECTRUM FUSION BASED NETWORK FOR MONAURAL
SPEECH ENHANCEMENT IN NASA MISSION BASED TEAM COMMUNICATION
Many NASA communications such as audio recordings from the Apollo missions contain
sever communications-based noise level, which pose substantial challenges for effective
human engagement as well as research tasks for subsequent team dynamics based
analysis.In this study, we propose an advanced denoising approach that combines both
signal processing expertise with deep learning algorithms.

February 13-16, 2024 345
Poster Session A: Muscle & Aerobic

176 Igor Mekjavic; Riccardo Sorrentino; Jack Fortune; Urša Ciuha; Lydia Tsoutsoubi; Leonidas
Ioannou; Jason Fisher; Sara Podgornik; Matija Šatej; Adam McDonnell
(1617099) RESISTANCE VIBRATION EXERCISE: A POTENTIAL COUNTERMEASURE
A 2-week training program of squat exercise and heel raises conducted on a vibration
platform during the application of artificial gravity on a short arm human centrifuge (SAHC)
bestowed significant benefits in lower leg muscle strength, when compared to a similar
training programme conducted in the upright position (ground reaction force was similar in
both exercise protocols).
177 Sandra Faragalla; Kaitlin Lostroscio; Leslie Quiocho; Charlotte Bell; Fouad Matari
(1640649) KINEMATIC SENSORS EVALUATION FOR SPACEFLIGHT EXERCISE DATA
COLLECTIONS
A kinematic accuracy evaluation of three motion tracking sensors to inform the most feasible
ISS exercise data collection method in the future and aid in understanding the sensors and
developing end-to-end processing.
178 Timo Frett; Leopold Lecheler; Michael Arz; Willi Pustowalow; Guido Petrat; Florian
Mommsen; Jan Breuer; Marie-Therese Schmitz; David Adrew Green; Jens Jordan
(1643501) ROWING IN ARTIFICIAL AND IN TERRESTRIAL GRAVITY: TOLERABILITY,
PERFORMANCE, AND PHYSIOLOGICAL RESPONSES
Rowing combined with artificial gravity via short-arm centrifugation was found to be feasible,
well-tolerated, and a potential strategy to mitigate cardiovascular and muscular
deconditioning during extended spaceflight, with certain differences in muscle activation and
ground reaction forces compared to terrestrial gravity rowing.
179 Cuiping Zhang; Peng Huang; Jeffrey Richards; Ye Zhang; Abba Zubair
(1643659) IMPACT OF SIMULATED MICROGRAVITY ON MESENCHYMAL STEM CELL
PROLIFERATION
Type of microgravity simulator and duration of culture significantly affect MSC proliferation.
180 Mariam Othman; Khaled Kamal; Joo Kim; Rachel Rauth; Lauren Wesolowski; Jessica
Simons; Pier Semanchik; Sarah White-Springer; Mariana Gomez; John Lawler
(1643843) THE SIRTUIN-1 AGONIST SRT2104 MITIGATES UNLOADING-INDUCED
SKELETAL MUSCLE ATROPHY AND INFLAMMATION
This study investigates whether the SIRT1 agonist SRT2104 mitigates inflammatory
signaling and mitochondrial dysfunction, thus reducing muscle atrophy induced by hindlimb
unloading.
181 Bergita Ganse; Danjana Teves
(1644101) IMMOBILIZED PATIENTS WITH (MULTIPLE) INJURIES AS A SPACEFLIGHT
ANALOGUE FOR MUSCLE ATROPHY - HIGH VARIABILITY, BUT GREAT AVAILABILTY
AND CLINICAL RELEVANCE
Immobilised patients with (multiple) injuries can serve as an analogue for studying muscle
atrophy if a large effect is expected, but due to the high variability compared to normal bed
rest studies with healthy participants, a much higher number of participants is required

February 13-16, 2024 355
182 Yoshinobu Ohira; Che Show Chen; Reggie Edgerton; Takuya Goto; Alan Hargens; Hisashi
Naito; Toshio Ohhata; Takashi Ohira; Masaki Takeda
(1644654) ESTIMATION OF WALKING PATTERNS AND METABOLIC RATES OF HUMAN
IN PARTIAL GRAVITY ENVIRONMENTS USING PARABOLIC FLIGHT AND ALTER-G
TREADMILL
Whole body oxygen consumption and electromyogram activities in soleus and lateral portion
of gastrocnemius during standing rest, stepping, and/or walking were recorded using
compact devices during the exposure to 1-G, 3/8-G, 1/5-G, or 1/6-G. The EMG activities of
both muscles during standing rest and stepping were decreased with reduction of gravity
level.
183 Nicole Strock; Brian Prejean; Alyssa Varanoske; Jason Norcross; Taylor Schlotman; Karina
Marshall-Goebel
(1645391) TEMPORAL CHANGES IN ASTRONAUTS' MUSCLE AND
CARDIORESPIRATORY PHYSIOLOGY BEFORE, DURING, AND AFTER SPACEFLIGHT
This flight study will provide valuable information for determining time course of change and
the interindividual variability of spaceflight-induced deconditioning of aerobic capacity and
muscle strength and endurance over the course of spaceflight missions up to and beyond 1
year.
184 Jonas Böcker; Guillaume Fau; Thomas Krüger; Jörn Rittweger; Torsten Siedel; Jochen
Zange
(1646104) ATHLETIC: AN EXOSKELETON COUNTERMEASURE DEVICE FOR
RESISTIVE AND PLYOMETRIC EXERCISE
The exoskeleton ATHLETIC device is a novel exercise device counteracting muscle and
bone loss for upcoming deep-space missions. Clinical tests showed the functionality and
efficacy of the device and first results comparing reference results with exercising on
ATHLETIC.
185 Jonas Böcker; Guillaume Fau; Jörn Rittweger; Arnaud Runge; Torsten Siedel; Jochen Zange
(1646113) NEX4EX - NOVEL APPROACH ENABLING RESISTANCE, SENSORIMOTOR
AND PLYOMETRIC TRAINING FOR DEEP-SPACE MISSIONS
Novel Exercise Hardware for Exploration (NEX4EX) is a countermeasure device enabling
sensorimotor training, resistive and plyometric training. The clinical tests evaluated the
efficacy and comparability of this novel approach and control measurements.
186 Alyssa Varanoske; Brian Prejean; Nicole Strock; Danielle Conly; Brian Peters; Erin Morant;
Jean Sibonga; Scott Smith; Sara Zwart; Elisabeth Spector; Renita Fincke; Millennia Young;
Karina Marshall-Goebel
(1646734) EFFECTS OF REPLACING TREADMILL RUNNING WITH ALTERNATIVE
EXERCISE COUNTERMEASURES DURING LONG-DURATION SPACEFLIGHT
This study will assess the efficacy of exploration exercise modalities, including the effects of
removing the treadmill exercise capability or of exclusively using the European Enhanced
Exploration Exercise Device (E4D), compared to nominal ISS exercise across an entire
mission on bone, muscle, aerobic, and sensorimotor health and performance.
187 Erik LeRoy; Brett Bennett; David Wassell
(1646759) AEROBIC TESTING LOGISTICS AS A PRE-FLIGHT COUNTERMEASURE FOR
MICROGRAVITY-RELATED VASCULAR DEGRADATION: A REVIEW
Optimizing maximal aerobic capacity closer to launch date will allow for less degradation of
aerobic fitness between test and launch date and subsequently during flight, as well as
getting ahead of the elastin degradation that takes place in microgravity during long duration
missions.

February 13-16, 2024 365
188 Evagelia Laiakis; Afshin Beheshti; Elizabeth Blaber; Emma Kosowski; Tytus Mak; Jeffrey
Willey
(1646976) ALTERATIONS IN ENERGY METABOLISM PATHWAYS IN SKELETAL
MUSCLE IN RELATION TO MICROGRAVITY ANALOG AND SPACE RADIATION
Metabolomics of muscles from combined injuries (radiation + hindlimb unloading) at 24 hours
after exposure suggest that radiation (acute exposures) leads to a higher metabolic
detriment, while hindlimb unloading either does not exacerbate the responses or the
responses reach a plateau.
189 Rachel Bellisle; Katya Arquilla; Lonnie Petersen; Andrea Webb; Dava Newman
(1647496) QUALITATIVE EVALUATION OF THE GRAVITY LOADING
COUNTERMEASURE SKINSUIT DURING EXERCISE AND FUNCTIONAL ACTIVITY IN
LOW EARTH ORBIT AND 1G
The abstract and poster discuss the qualitative evaluation of the Gravity Loading
Countermeasure Skinsuit, a proposed wearable musculoskeletal and sensorimotor
countermeasure, with one participant on a 10-day ISS mission, supplemented by a
participant study (n≥3) in 1G.
190 Eve Elmore; Lance Bollinger; John Caruso; Stuart Best; Timothy Butterfield
(1648838) EFFECTS OF UNILATERAL LOWER LIMB SUSPENSION OF THE
QUADRICEPS STRUCTURE AND FUNCTION
Muscle unloading during space flight decreases strength and cross-sectional area of anti-
gravitational muscles, such as the quadriceps. Purpose is to assess changes in quadriceps’
size, anisotropy, and function following unilateral limb suspension (ULLS), where one limb is
unloaded and the contralateral limb acts as an internal (loaded) control.
191 Khaled Kamal; Mariam Othman; Joo-Hyun Kim; Jacob Kendra; Shadi Golpasandi; Rachel
Rauth; Aaron Morton; John Lawler
(1650211) ENHANCING SKELETAL MUSCLE RECOVERY AND MITIGATING ATROPHY
IN DISUSE: A NOVEL APPROACH USING TIME RELEASE ION MATRIX (TRIM)
APPLICATION
A biocompatible ceramic called TRIM has potential to mitigate spaceflight induced muscle
atrophy, enhance growth factors, and reduce oxidative stress and inflammation, ultimately
increasing muscle size and accelerating recovery after unloading.
192 John Lawler; Joo-Hyun Kim; Khaled Kamal; Mariam Othman; John Ford; Yuxiang Sun;
Rachel Rauth; James Fluckey
(1650458) WE HAVE IGNITION: REDOX REGULATION OF MECHANOTRANSDUCTION
WITH SPACEFLIGHT AND TRANSLATION TO MYOPATHIES ON EARTH
The redox biology of mechanotransduction during spaceflight is regulated by positive
feedback loops, that include RANKL and effectors of oxidative stress and inflammation,
amplifying pathology with spaceflight, aging, and Duchenne muscular dystrophy.
193 Matthew McDonnell; Cody Burkhart; Nathaniel Jenkins; Jeevan Perera
(1650878) QUANTIFYING CALORIC EXPENDITURE DURING ZERO-G EXERCISE
This study aims to develop a user-friendly predictive model to measure calories burned
during exercise aboard the International Space Station, using spirometry, heart rate, forces
and more, with the goal of improving health and wellness for astronauts on extended space
missions.
194 Kelly Crowe; Brianna Mackey; Nate Mazza; Robert Cullen; Marie Mortreux
(1652522) SKELETAL MUSCLE SIALYLATION IN SIMULATED MICROGRAVITY
This preliminary data shows alterations in the extracellular glycans of rat skeletal muscle
after exposure to hindlimb unloading, which could represent a novel target for
pharmacological and nutraceutical interventions to address skeletal muscle atrophy in
spaceflight conditions.

375
Poster Session A: Research Operations & Integration (ROI)

162 Meredith Russell; Lauren Secino; Sami Anjum
(1647595) NASA ROI PROSPECTIVE DATA SHARING PROCESS OVERVIEW OF
SPACEFLIGHT STUDIES
Learn how to navigate the new Research Operations and Integration (ROI) data sharing
process from the perspective of a researcher including best practices and frequently asked
questions.
163 Oscar Ojeda; Samuel Cadavid; Yael Méndez; Camilo Zorro
(1650455) DEVELOPMENT OF A HUMAN SPACEFLIGHT ANALOG RESEARCH
PROGRAM IN A NON SPACE-FARING NATION
This work presents the development of an analog research program in Colombia, centered
around the HAdEES-C station, built north of Bogotá, which allows analog missions to be
undertaken. A description of the facilities, the activities developed, and the importance for
non-space-faring nations to develop these activities is presented, as well as the undergoing
projects and cooperation opportunities.
164 Katherine Rahill; Alexandra Whitmire; Ajitkumar Mulavara
(1691331) REVIEW OF SCIENTIFIC REQUIREMENTS FOR HIGH-FIDELITY LUNAR AND
MARTIAN RESEARCH ANALOGS
This presentation highlights the importance of analog research for human spaceflight,
emphasizing analogs’ critical role in preparing for Artemis and Mars missions, particularly in
regard to buying down behavioral health and performance risks.

February 13-16, 2024 385
Poster Session A: Sensorimotor

195 Caroline Austin; Beth Lewandowski; Jerry Myers; Hunter Rehm; Scott Wood
(1627322) CHP-PRA: SENSORIMOTOR COUNTERMEASURES PROOF OF CONCEPT
This effort demonstrates the use of countermeasure data to inform performance risk using
sensorimotor data as an example.
196 Faye Tan; Jeffrey Strakowski; Han Zhang; Millard Reschke
(1629141) HIGH FREQUENCY ULTRASOUND IMAGING OF CADAVERIC INNER EAR
STRUCTURES
The study demonstrates that pertinent anatomic structures of the inner ear as well as their
positional relationships can be reliably identified with high frequency ultrasound to facilitate
appropriate beam positioning toward the targets of interest with dissected windows in
cadaveric specimens.
197 Caroline Austin; Torin Clark
(1641986) SIMULATING POSTFLIGHT VESTIBULAR ILLUSIONS WITH GALVANIC
VESTIBULAR STIMULATION
This project investigates the effect of Galvanic Vestibular Stimulation on orientation
perception and how it may be optimized to mimic astronaut post-flight vestibular illusions and
functional mobility performance.
198 Torin Clark; Taylor Lonner; Caroline Dixon; Sherrie Holder; Jordan Dixon; Tristan Endsley
(1642031) DETECTING PILOT SPATIAL DISORIENTATION TO TRIGGER ACTIVE
COUNTERMEASURES DURING LUNAR LANDING
We aim to develop a pilot aiding system to detect spatial disorientation in real-time during
lunar landing and trigger a countermeasure to maintain performance and safety.
199 Taylor Lonner; Aaron Allred; Aadhit Gopinath; Luca Bonarrigo; Torin Clark
(1643095) ACTIVE POSTURAL CONTROL AND ANTICIPATORY CUEING AS
COUNTERMEASURES FOR ASTRONAUT MOTION SICKNESS DURING WATER
LANDINGS
Two novel, non-pharmacological countermeasures are evaluated for efficacy in reducing the
incidence and severity of astronaut motion sickness and sensorimotor degradation following
a water-landing analog.
200 Timothy Macaulay; Scott Wood; Millard Reschke; Yiri De Dios; Joseph Dervay; Tomoko
Makishima; Michael Schubert; Mark Shelhamer; Amir Kheradmand; Gilles Clement
(1644351) NEURO-VESTIBULAR EXAMINATION DURING AND FOLLOWING
SPACEFLIGHT (VESTIBULAR HEALTH)
This study represents the Sensorimotor arm of the Complement of Integrated Protocols for
Human Exploration Research (CIPHER) and aims to characterize the severity of vestibular
syndromes experienced by astronauts as a function of spaceflight duration and examine
whether the effects are caused by changes at the peripheral end organs, midbrain,
cerebellum, or vestibular cortex.
241 Timothy Macaulay; Seward Rutkove; Brian Peters; Lars Oddsson; Scott Wood; Leah Bent;
Francisco Valero-Cuevas; Emily Lawrence; Nicole Strock; Millennia Young; Jacob
Bloomberg; Marie Mortreux; Mary Bouxsein; Afshin Beheshti; Thomas Abitante; Dava
Newman
(1644498) COUNTERMEASURES FOR MITIGATION OF SENSORIMOTOR
DECREMENTS FOLLOWING HEAD-DOWN BED REST
This study will evaluate the efficacy of sensorimotor countermeasures (proprioceptive
training and electrical muscle stimulation) performed during head down bed rest (HDBR) for
protecting functional task performance, sensorimotor function, and other key physiological
measures following 60 days of 6° HDBR.

February 13-16, 2024 395
242 Hannah Weiss; Sarah Moudy; Scott Wood

(1644918) DEVELOPMENT OF HEAD-TRUNK COORDINATION MEASURES FOR
MONITORING POSTFLIGHT SENSORIMOTOR READAPTATION STRATEGIES
To better characterize microgravity-induced sensorimotor adaptations, we investigated head-
trunk coordination measures that are sensitive enough to detect coupling changes by
comparing sensitivity of these measures with and without vestibular disorientation via
Galvanic Vestibular Stimulation (GVS) and physical restriction via a neck brace during short
duration testing.
243 Victoria Kravets; Aadhit Gopinath; Torin Clark
(1645212) QUANTIFYING TEMPORAL DYNAMICS OF NEUROVESTIBULAR
ADAPTATION TO ALTERED GRAVITY
This research investigates how humans adapt to changes in the magnitude of gravity,
specifically focusing on neurovestibular impairment during the first hour after transitioning
from normal Earth gravity to a higher gravity environment, using centrifugation and subjective
perception measurements.
244 Austin Bollinger; Kevin Duda; Steven Moore; Douglas Wheelock; Scott Wood
(1645216) MANUAL CREW OVERRIDE OF VEHICLE LANDINGS FOLLOWING G-
TRANSITIONS
In this study, we will examine operational piloting tasks following International Space Station
(ISS) missions in a simulated lunar landing using a six-degree-of-freedom (6DOF) motion
base.
245 Olivier Etard; Olga Kuldavletova; Mikael Naveau; Gaëlle Quarck; Antoine Langeard; Marion
Hay; Adela Kola; Michel Toupet; Christian van Nechel; Charlotte Hautefort; Pierre Denise;
Gilles Clément
(1646168) CAN IDIOPATHIC BILATERAL VESTIBULOPATHY BE A GROUND-BASED
MODEL FOR LONG DURATION SPACEFLIGHT?
Patients with bilateral vestibular deficits could be a model of the effects of long-duration
space travel, and would thus facilitate understanding of the mechanisms involved, in
particular by exploring the role of the hippocampus in the link between vestibular perception
and impaired postural control.
246 Grant Tays; Bianca Arocha; Rachael Seidler; Scott Wood
(1646949) THE EFFECTS OF SENSORY BIAS AND TRANSCRANIAL DIRECT CURRENT
STIMULATION ON FUNCTIONAL MOBILITY TEST ADAPTATION AS A POST-FLIGHT
COUNTERMEASURE
We investigate the use of transcranial direct current stimulation over the human vestibular
cortex, as vestibular mediated performance deficits are common following spaceflight, on
functional mobility test performance as a post-flight countermeasure.
247 Grant Tays; Tyler Fettrow; Heather McGregor; Kathleen Hupfeld; Yiri De Dios; Nichole
Beltran; Scott Wood; Patricia Reuter-Lorenz; Jacob Bloomberg; Rachael Seidler
(1647230) FUNCTIONAL BRAIN NETWORK SEGREGATION PREDICTS POST-FLIGHT
BALANCE IMPAIRMENTS
We assessed pre-flight vestibular and visual functional brain network segregations ability to
serve as predictor of post-flight balance impairment and found that visual segregation
predicts post-flight balance perform 1 day after returning to Earth, where vestibular pre-flight
segregation predicted balance performance 4 days after returning to Earth; both networks
had high test-retest reliability.
248 Matthew Carey; Matthew Ehrenburg; Tim Macaulay; Scott Wood
(1647946) COMPARISON OF ACTIVE AND PASSIVE HEAD IMPULSE TESTING OF THE
HORIZONTAL VESTIBULO-OCULAR REFLEX: EXPLORING THE FEASIBILITY OF
DIFFERENT APPROACHES FOR SPACEFLIGHT
As a part of the CIPHER Vestibular Health project, this study aimed to test the reliability of
Head Impulse Testing (HIT) between different operators and to compare passive (pHIT) vs
active (aHIT) administration, gaining a better understanding of HIT efficiency and reliability in
quantifying vestibular function before, during, and after human spaceflight.

February 13-16, 2024 405
249 Hui Ho Vanessa Chang; Kathleen Cullen

(1648113) THE MOUSE NUCLEUS PREPOSITUS RELAYS EYE MOVEMENT
INFORMATION TO HEAD DIRECTION NETWORK DURING NAVIGATION
Through studying the mouse NPH, we found that eye movements, more than head
movements, significantly influence the neural encoding of direction, highlighting the
vestibular system's intricate role in spatial awareness.
250 Vivekanand Vimal
(1649307) COMMUNITY ENGAGEMENT THROUGH EDUCATION, SENSORY
AUGMENTATION DEVICES, FASHION AND DANCE ON THE TOPIC OF SPATIAL
DISORIENTATION AND VIBROTACTILE FEEDBACK
In this poster, I will share both traditional educational outreach initiatives along with more ‘out
there’ ideas that dip into the wildness of unfettered creativity.
251 Catho Schoenmaekers; Elisa Raffaella Ferre; Floris L. Wuyts
(1649352) MULTISENSORY INTEGRATION FOR VERTICALITY PERCEPTION
The brain constructs a representation of verticality by integrating vestibular and visual
information. However, how mechanistically these sensory cues are integrated for the
perception of verticality has been largely debated. Here we systematically investigated the
dynamic integration of vestibular and visual cues, considering their respective reliability, in
the perception of verticality.
252 Akshay Kothakonda; Megan Reissman; Timothy Reissman; Torin Clark; Faisal Karmali
(1650115) SPATIAL DISORIENTATION PREDICTION THROUGH CLOSED-LOOP
MODELS OF HUMAN-VEHICLE SYSTEM
This work models spatial disorientation during vehicle acceleration and resulting erroneous
pilot inputs, in order to develop appropriate training and countermeasures posed by this risk.
253 Scott Wood; Yiri De Dios; Tim Macaulay; Brian Peters; Nichole Beltran; Vincent Koppelmans;
Torin Clark; Rachael Seidler; Lars Oddsson; Corey Theriot; Jacob Bloomberg
(1650161) ASSESSING THE RELATIONSHIPS BETWEEN SENSORIMOTOR
BIOMARKERS AND POST-LANDING FUNCTIONAL TASK PERFORMANCE
The goal of the Sensorimotor Predictors Study is to identify a set of behavioral, neuroimaging
and genetic measures that can be used to predict early post-flight performance on a set of
sensorimotor tasks.
254 Mimi Lan; Darin Knaus; Shireen Geimer; Lionel Lewis; Samantha Leigh; Jay Buckey
(1650223) INTRANASAL ADMINISTRATION OF AQUEOUS SCOPOLAMINE WITH A
NEBULIZER MAY PROVIDE RAPID MOTION-SICKNESS RELIEF
Intranasal administration of aqueous scopolamine using a nebulizer may provide rapid
motion-sickness relief.
255 John Hayes; Eshan Manchanda; Blake Fairchild; Mark Morrison; Scott Wood; Ranjana
Mehta
(1650266) EXAMINING THE INTERACTIONS OF MENTAL FATIGUE AND VESTIBULAR
CHALLENGES ON SENSORIMOTOR PERFORMANCE
This study examined the interaction effects of vestibular impairment (modeled by galvanic
vestibular stimulation) and mental fatigue on sensorimotor performance (static balance,
dynamic balance, and upper body motor control).
256 Mukul Mukherjee; Joel Sommerfield; Takashi Sado; Christopher Engsberg; Aaron Likens
(1650515) MACHINE LEARNING TOOLS FOR PREDICTING TASK CONDITIONS FROM
ALTERATIONS IN WALKING BEHAVIOR IN ASTRONAUTS
Machine learning tools are used to predict environmental or task conditions from human
behavioral metrics.

415
257 Amrita Mukunda; Daniel Ludwig; Shrutina Shrestha; Charles Stewart; Scott Wood; Michael
Schubert
(1650654) SELF-ADMINISTERED REHABILITATION TOOL APPEARS TO ENHANCE
SENSORIMOTOR RECOVERY MORE QUICKLY THAN TRADITIONAL REHABILITATION
I provide background, methods and results on gait assessment in a study comparing
incremental self-administered rehabilitation compared to traditional rehabilitation in those
who have had an eighth cranial nerve resection.
258 Shrutina Shrestha; Danny Ludwig; Scott Wood; Amrita Mukunda; Charles Stewart; Michael
Schubert
(1650762) SELF-ADMINISTERED REHABILITATION TOOL APPEARS TO MITIGATE
POST SURGICAL PERCEPTION OF OCULOMOTOR ALIGNMENT
Ocular alignment testing to quantify otolith asymmetry was completed in 9 of 24 patients,
recovering from unilateral vestibular nerve resection and undergoing novel self-
administration rehabilitation, as an analogue for astronauts in long-duration spaceflights who
experience abnormal roll tilt due to extended exposure to microgravity.

February 13-16, 2024 425
Poster Session A: Student Travel Award Winners

011 Kylee Magee
(1706558) HUMAN RESEARCH PROGRAM STUDENT TRAVEL AWARDS - RESEARCH
AND CAPABILITIES (MAGEE)
As a recipient of the NASA Human Research Program Student Travel Award, this
undergraduate student will present a poster with information about research or capabilities at
their respective institutions relevant to space life sciences.
012 Tommy Mounarath
AND CAPABILITIES (MOUNARATH)
013 Samer Ali
AND CAPABILITIES (ALI)
014 Kim Ngan Ngo
AND CAPABILITIES (NGO)
015 Yanaba Hatathlie
AND CAPABILITIES (HATATHLIE)

435
Poster Session A: Translational Research Institute of Space Health (TRISH)

259 Jon Rittenberger; Brian Clemency; Brian Monaco; Jacqueline Schwob; Joshua Murphey;
David Hostler
(1606574) COMPARING HYPOTHERMIC AND THERMAL NEUTRAL CONDITIONS TO
INDUCE METABOLIC SUPPRESSION IN HEALTHY SUBJECTS
In healthy individuals, both sedation and a cold environment are required to induce metabolic
suppression.
260 Marie Mortreux; Samantha Dworacek; Grace Brucato; Kate Flickinger; Alexandra Weissman;
Clifton Callaway; Kathleen Melanson
(1628923) OPTIMIZED NUTRITION TO SUPPORT METABOLIC SHIFTS AND MUSCLE
HEALTH IN A NEW HUMAN SPACEFLIGHT ANALOG
Using prolonged cold-sleep to reduce the metabolic needs of astronauts will be useful during
the journey to Mars but might compromise skeletal muscle health; here we propose to use an
optimized diet to support both metabolic suppression and muscle health.
261 Anders Eklund; Tomas Bäcklund; Jan Malm; Michael Williams
(1641574) BENCH TESTING OF THE M.SCIO AND LUMBAR CATHETER FOR THE PILOT
FEASIBILITY STUDY OF SAFETY AND EFFECTIVENESS OF TELEMETRIC ICP IN
ASTRONAUTS BEFORE, DURING, AND AFTER COMMERCIAL ORBITAL SPACEFLIGHT
Astronauts in microgravity develop ocular symptoms that raise suspicion of elevated ICP, to
prepare for ICP measurements at ISS we performed a bench test to evaluate a Telemetric
ICP Monitoring system for lumbar access, we found that it is feasible, but for assessment of
ICP pulse amplitude it is important to use shortest possible catheter and post processing to
reduce the effect of noise.
262 Marie Migaud; Trey Deason; Kaveri Goel; Faisal Hayat; Arlet Hernandez; A. Kibbe; Brice
Nickels; Janice Pluth
(1642132) CONTROLLING NAD(P) HYPER-OXIDATION TO REGULATE REPAIR AND
MAINTENANCE PROCESSES IN HUMAN IN SPACE
This presentation will reveal a fundamental and multi-modal cause of dysfunction in
endogenous repair and cellular maintenance processes in response to the hyper-oxidation of
the redox cofactor NAD by ROS-inducing space-like conditions and provides a roadmap to
the means to mitigate it.
263 Alexander Fliflet; Nicholas Burd; Hyunjoon Kong; Justin Rhodes; Taher Saif; Jonathan
Sweedler; Marni Boppart
(1643234) DESIGN OF AN EXTRACELLULAR VESICLE APPROACH TO PROTECT
HUMAN HEALTH IN SPACE
The primary objective of this project is to design a non-cell specific, blood-derived
extracellular vesicle therapy to protect human health in space.
264 Stuart George; Ramona Gaza; Nicholas Stoffle
(1643539) RADIATION MEASUREMENTS WITH HERA ON SPACEX POLARIS DAWN
The authors present detailed radiation measurements in a 1400km orbit from the SpaceX
Polaris Dawn flight (if it has successfully flown by the presentation date).
265 Arjun Yogaratnam; Katie Chong; Shrutina Shrestha; Mark Shelhamer; Mallika Sarma
(1644055) PHYSIOLOGICAL SYNCHRONY IN DYADIC PAIRS DURING HIGH-STRESS
DECISION MAKING TASKS
In determining whether stressful mission-like conditions are enough to evoke synchrony in
heart rate in dyadic teams, along with how this synchrony may affect performance, we found
that despite the variability, dyads with the appearance of greater levels of synchrony in both
heart rate and heart-rate variability seemed to progress further in the high-stress decision-
making task.

February 13-16, 2024 445
266 Adrien Robin; Yasmin Zaman; Nastassia Navasiolava; Marc-Antoine Custaud; David
Zawieja; Ana Diaz-Artiles
(1644833) EFFECT OF DECONDITIONING ON GRAVITATIONAL DOSE-RESPONSE
CURVES FOR CARDIOVASCULAR AND OCULAR VARIABLES IN A TILT PARADIGME
A graded tilt protocol to generate gravitational dose responses curves will be performed on
32 healthy subjects (16M/16F) before and after 24h head-down bed rest and furosemide-
induced hypovolemia, to contribute to the assessment of the headward fluid shift, SANS,
venous thromboembolism events, and to the development of countermeasures
267 Lisa Hanson; Kristina Henry Collins; Sara Torres
(1645042) LBJ SPACE HEALTH INCLUSION PROJECT
The underrepresentation issues afflicting the space health research community are akin to
those of the broader STEM communities - females and students from historically
underserved populations remain underrepresented.
268 Marcos Negrete-Obando; Sharon Gerecht
(1647062) HUMAN 3D BLOOD VESSEL MODEL REVEALS RADIATION MODULATION OF
ENDOTHELIAL CELL PHENOTYPE AND FUNCTION
We developed and characterized a novel 3D human vascular tissue model derived from
human induced pluripotent stem cells (hiPSCs) and analyzed damage caused by ionizing
radiation. We studied high mobility box-1 (HMGB-1) activation in endothelial cells (EC) as a
potential target for countermeasures. This 3D model may lead to a better understanding of
the effects of space radiation.
269 Ashley Nemec-Bakk; Marjan Boerma; Jeffrey Willey; Igor Korturbash; Chirayu Patel; Alex
Borg; Vijayalakshmi Sridharan; Gabriel Gifford; Wayne Newhauser; Abdelrahman Fouda;
Jacqueline Williams; Jeffery Chancellor
(1647575) EFFECTS OF GALACTIC COSMIC RADIATION AND SIMULATED
MICROGRAVITY ON THE CARDIOVASCULAR SYSTEM AND RETINA
Male and female C57BL/6 mice exposed to simulated simplified galactic cosmic radiation
while simultaneously hindlimb unloaded demonstrated minor changes in cardiac and retina
structure.
270 Megan Waldock; Kim Ayers
(1647662) HEALTH RESEARCH PLATFORM - YET2 TECHNICAL SURVEY
From February 2023 to April 2023, yet2, a technology scouting and open innovation
consulting company, worked with NASA TRISH to identify autonomous, closed-loop health
management platforms able to collect and store medical and biomedical research data for
astronauts as they move between vehicles during their spaceflight journey.
271 Daniel Norena-Caro; Jenna Armstrong; Changqi Liu; Jing Zhao; Cristal Zuniga
(1647833) ENHANCING THE FLAVOR AND NUTRITIONAL VALUE OF ALGAE-ENRICHED
SPACE GUACAMOLE USING THE METABOLIC NETWORK ANALYSIS OF DUNALIELLA
SALINA
Phenotyping and biomass composition data are combined with a genome scale metabolic
model to simulate growth conditions that enhance antioxidant, taste, and olfactory properties
of Dunaliella salina as an ingredient of freeze-dried guacamole.
272 Shanice Taylor; Karen McDonald; Somen Nandi; Noah Langenfeld; Bruce Bugbee
(1648020) EXPRESSION OF PROTEIN BIOLOGICS IN TRANSGENIC LETTUCE TO
TREAT MICROGRAVITY-RELATED OSTEOPENIA
Expression of the human Parathyroid Hormone-Fc protein in transgenic lettuce for potential
to treat microgravity-induced osteopenia in space.
273 Aidan Zerdoum; Clifton Garner; Thew Yeager; Douglas Ebert; Ashot Sargsyan
(1648117) DEVELOPMENT OF AN ULTRASOUND IMAGE SIMULATOR: IMPROVING
EDUCATIONAL EFFICACY AND MEDICAL REALISM IN ULTRASOUND TRAINING
Development and refinement of an advanced ultrasound image simulator which enhances
training realism by incorporating real-time image generation with an anatomically relevant
domain space, offering potential benefits for spaceflight medical procedure training.

February 13-16, 2024 455
274 Aidan Zerdoum; Theodora Bock; Clifton Garner; Douglas Ebert; Ashot Sargsyan
(1648143) UTILIZING GAME DESIGN TO ENHANCE SONOGRAPHY TRAINING: A
COMPREHENSIVE APPROACH
Discussion of specific gameplay elements which were incorporated into the "Fundamentals
of Ultrasound: Artemis Training Solution" game and their facilitation of heightened
engagement, emphasizing hands-on learning.
275 Michael Funke; Fernando Maestu; Patrique Fiedler; Ricardo Bruña; Pablo Cuesta;
Christopher Laohathai; Sandra Pusil; Adrian Quivira-Lopesino; Maria Sevilla-Garcia
(1648265) BRIEF REVIEW OF EEG UTILIZATION IN HUMAN SPACEFLIGHT
Despite the findings of previous inflight EEG studies and the importance of surveying
cognitive and mental health status during spaceflight missions, EEG was - and still is - an
underappreciated and underutilized tool in spaceflight, and there is no unified pathway to
access existing EEG data for space flight research ort preventing loss of such valuable data
like the Gemini 7 EEG recordings.
276 Ricardo Bruña; Patrique Fiedler; Michael Funke; María Garcia-Sevilla; Jens Haueisen;
Fernando Maestú; Sandra Pusil; Adrián Quivira-Lopesin
(1648424) DRY EEG FOR FUNCTIONAL CONNECTIVITY ASSESSMENT IN
SPACEFLIGHT
Here we evaluate a dry cap to estimate power and functional connectivity in a real setup,
with the aim of using such systems to monitor astronauts' brain health during space missions
277 Ricardo Bruña; Pablo Cuesta; Patrique Fiedler; Michael Funke; Jens Haueisen; Fernando
Maestú; Adrián Quivira-Lopesin; María Sevilla-García
(1648440) RELIABILITY OF A HIGH-DENSITY DRY-EEG CAP TO ESTIMATE
FUNCTIONAL CONNECTIVITY IN SOURCES SPACE
Here we evaluate a high-density dry EEG cap to estimate functional connectivity in sources
space in a real setup, with the aim of using such systems to monitor astronauts' brain health
during space missions
278 Rachael Seidler; Christine Wegner; Ana Diaz-Artiles; Catherine Domingo; Josephine Allen
(1648461) B-SURE: BOOSTING SPACEFLIGHT UNDERREPRESENTED RESEARCHER
EQUITY, A TRISH DIVERSITY PARTNERSHIP
We will present program outputs and outcomes from the Translational Research Institute for
Space Health diversity partnership, B-SURE: Boosting Spaceflight Underrepresented
Researcher Equity.
279 Miguel Jimenez; Emily Kolaya; Gary Liu; Giovanni Traverso
(1648515) MICROBIAL DEVICES: INTEGRATING MICROBES AND MATERIALS FOR
SPACE HEALTH
We are developing microbial devices that could be used to generate medicines on demand,
improve plant growth and monitor health during exploration space travel.
280 Adrian Quivira-Lopesino; Maria Sevilla-Garcia; Pablo Cuesta; Sandra Pusil; Ricardo Bruña;
Patrique Fiedler; Fernando Maestu; Michael Funke
(1648549) EEG ALPHA POWER COMPARISON BETWEEN DATA FROM HEAD-DOWN
BED REST AND DURING SPACEFLIGHT IN THE DEFAULT MODE NETWORK
The head-down bed rest position (HDBR) mimics spaceflight effects due to microgravity.
Although the results of the HDBR and ISS recordings showed similar tendencies, there are
statistical differences between them. The alpha reduction severity at the ISS recordings
could be because of the presence of additional factors, or because the microgravity
comparison between HDBR and ISS is not effective.
321 Mathias Basner; Adrian Ecker; Yoni Gilad; Christopher Jones
(1648801) COGNITIVE AND PHYSIOLOGIC RESPONSES IN COMMERCIAL SPACE
CREW ON SHORT-DURATION MISSIONS
As part of TRISH Essential Measures, we collect cognitive and physiologic data with
computerized tests (Cognition battery) and wearables on commercial crew flights, the latest
being Ax-1 and Ax-2.

465
322 Angel Rodriguez; Erika dIRM Carbrera Ranaldi; Alen Saju; Deepa Roy; Mario Gil; Xiao Wen
Mao; Juan Pablo de Rivero Vaccari; Upal Roy
(1648926) EFFECTS OF SPACEFLIGHT ON INFLAMMASOME SIGNALING AND
NEUROINFLAMMATION
Space flight environment associated with space exploration results in risks to astronauts,
including inflammasome dysregulation, so this study aims to understand inflammasome
signaling in the brain of mice exposed to space flight conditions.
323 Jing Zhao; Ali Raza; Joanna Rockwell; Changqi Liu; Cristal Zuniga
(1649172) NUTRITIOUS AND FLAVORFUL MICROALGAE-ENRICHED GUACAMOLE MIX
FOR SPACE EXPLORERS
Our project aims to develop a shelf-stable, flavorful, convenient, and nutrient dense
guacamole dry mix enriched with microalgae, which can be enjoyed following rehydration.
324 Patrique Fiedler; Michael Funke; Fernando Maestú; Pablo Cuesta; Ricardo Bruña; Ana Maria
Cebolla Alvarez; Guy Cheron; Maria Sevilla-Garcia; Jens Haueisen
(1649258) EEG SIGNAL QUALITY AND NOISE CHARACTERISTICS IN SPACEFLIGHT
To date, no systematic investigations on signal quality and eventual interferences in
spaceflight environments have been reported. We compared the signal characteristics of
spaceflight and on-earth EEG to identify eventual shortcomings. We conclude that EEG
recordings during spaceflight are possible without considerable environmental noise
interference differences impacting the data analysis.
325 Maria Sevilla-Garcia; Adrian Quivira-Lopesino; Pablo Cuesta Prieto; Ricardo Bruña; Sandra
Pusil; Patrique Fiedler; Guy Cheron; Ana Maria Cebolla Alvarez; Katharina Brauns;
Alexander Stahn; Michael E Funke; Fernando Maestu
(1649526) FUNCTIONAL CONNECTIVITY COMPARITION BETWEEN HEAD-DOWN TILT
POSITION AND MICROGRAVITY DURING SPACEFLIGHTS IN THE DEFAULT MODE
NETWORK
This study compares Default Mode Network brain functional connectivity during spaceflight
and head-down tilt bed rest, revealing significant differences with a more pronounced
reduction during spaceflight, raising questions about if the FC decreasing is not only caused
because of microgravity and about the adequacy of bed rest as a model for space-induced
neurophysiological changes.
326 Pablo Cuesta Prieto; Adrián Quivira-Lopesino; María Sevilla-Garcia; Sandra Pusil; Ricardo
Bruña; Patrique Fiedler; Ana María Cebolla; Guy Cheron; Katharina Brauns; Alexander
Stahn; Fernando Maestú; Michael Funke
(1649739) BRAIN SPECTRAL POWER ALTERATIONS ASSOCIATED WITH HEAD-DOWN
TILT BED REST POSITION
A comprehensive data-driven characterization of all topological changes in the anatomy and
frequency of spontaneous electromagnetic activity during a head-down tilt bed rest position
(HDBR) experiment.
327 Lindsay Altidor; Sivan Osenberg; Luke Parkitny; Kevin Lei; Srinivas Chamakuri; Lawrence
Bronk; Fada Guan; Mostafa Gaber; David Grosshans; David Young; Mirjana Maletic-Savatic
(1649861) EVALUATING NR2E1 AGONISTS AS POTENTIAL NEW THERAPEUTICS FOR
RESCUING RADIATION-INDUCED EFFECTS ON NEURAL STEM CELLS AND
NEUROGENESIS
We used human brain organoids to evaluate NR2E1 agonists as potential new therapeutics
for rescuing radiation-induced effects on neural stem cells and neurogenesis.
328 Danielle Yarbrough; Sharon Gerecht
(1649910) GALACTIC COSMIC RADIATION DIRECTLY INDUCES SMOOTH MUSCLE
SENESCENCE IN TISSUE ENGINEERED SMALL ARTERY MODEL
A tissue-engineered in vitro model of a small artery with human smooth muscle tissue was
exposed to Galactic Cosmic Radiation, and a senescent secretory phenotypic shift was
directly induced on both a tissue level and transcript level in smooth muscle cells, allowing
for potential identification of therapeutic targets for mitigating radiation-induced
cardiovascular disease.

February 13-16, 2024 475
329 Michael Williams; Karen Adams; Elisa McGee

(1650495) REGULATORY FEASIBILITY STUDY OF IMPLANTATION OF A TELEMETRIC
ICP SENSOR IN COMMERCIAL SPACEFLIGHT PARTICIPANTS
We present the final report of our regulatory application to the FDA for proposed research to
use a surgically implanted telemetric ICP sensor in a commercial spaceflight participant.
330 Michael Williams; Karen Adams; Elisa McGee; Robert Bonow; Andrew Ko; Danial Hallam;
Courtney Gomez; J. Eric Schmitt; Marissa Rosenberg; Jaime Mateus; Christoph Miethke;
Andreas Bunge; Eric Bershad
(1650568) PILOT FEASIBILITY STUDY OF SAFETY AND EFFECTIVENESS OF
TELEMETRIC INTRACRANIAL PRESSURE MONITORING IN ASTRONAUTS BEFORE,
DURING, AND AFTER COMMERCIAL ORBITAL SPACEFLIGHT
We present the progress of our research to use a surgically implanted telemetric ICP sensor
in a commercial astronaut.
331 Adrian Specogna
(1650625) GENERATING REALISTIC SYNTHETIC DATA USING GENERATIVE NEURAL
NETWORKS: AN EXAMPLE USING REAL PHYSIOLOGIC AIRLINE PILOT DATA
This study uses a generative neural network architecture, to create realistic synthetic data,
from real physiologic measurements, obtained from airline pilots.
332 Binh Dang; Thong Le; Hoang Tran; Hargsoon Yoon
(1650664) HIGH-SPEED CUSTOM FPGA-BASED SPI-TO-ETHERNET BRIDGE FOR
ACCELERATING REAL-TIME ELECTRICAL IMPEDANCE TOMOGRAPHY SYSTEMS
The paper is to implement a data carrier system between Serial Peripheral Interface (SPI)
and 100Mbps Ethernet Physical Layer (PHY), customized for the EIT system for low-cost,
high bandwidth, upgradability, and reliable data transmission between the ADCs and
controller units.
333 Hoang Van Tran; Vu Huu Pham; Thong Chi Le; Hargsoon Yoon
(1650700) MEDICAL IMAGING OF THE HUMAN BRAIN UNDER DIFFERENT GRAVITY
FORCES USING ELECTRICAL IMPEDANCE TOMOGRAPHY
In this research, we employed our custom-built Electrical Impedance Tomography (EIT)
system to generate three-dimensional medical images of a simulated human head model,
often called a "human head phantom," while subjecting it to varying gravitational conditions.
334 Stephanie Dudzinski; Stephanie Dudzinski; Li Wang; Jane Lea Hicks; Usha Andley; Bita
Esmaeli; Benjamin Frankfort; Michael Spiotto; Steven Frank
(1670650) CHARACTERIZING RADIATION INDUCED PRO-INFLAMMATORY RESPONSE
AND IMMUNOMODULATING EFFECTS OF RADIATION MITIGATING THROMBIN
PEPTIDE
Many ocular symptoms that arise in astronauts are radiation-induced, and we hypothesis that
space radiation promotes a pro-inflammatory immune microenvironment in the eye resulting
in loss of vision. TP508, a thrombin peptide, can mitigate this pro-inflammatory response,
reducing inflammation and initiating a wound-repair and recovery response that will prevent
ocular disease and dysfunction.
335 Emily Aurand; Kaitlyn Duvall; India Hook-Barnard
(1677930) A TECHNICAL RESEARCH ROADMAP FOR ENGINEERING BIOLOGY AND
SPACE HEALTH
With support from TRISH, the Engineering Biology Research Consortium (EBRC) is leading
a community-based production and publication of a technical research roadmap on
engineering biology for space health.

February 13-16, 2024 485
Wednesday, February 14, 2024
Biological Insights into Space Radiation Carcinogenesis
Chairs: Honglu Wu and Michael Weil

9:00 AM William Dynan; Alexis Chan; Lahcen Jaafar
(1648562) RETHINKING LUNG CANCER RISK: NO EFFECT OF SIMULATED GCR
EXPOSURE ON LUNG TUMOR INCIDENCE IN C57/BL6 MICE
Lung tumor incidence at 18 months post-treatment was not significantly different from
unexposed baseline in cohorts of mice exposed to simulated galactic cosmic radiation,
gamma rays, or silicon ions at doses ranging from 0.75 to 1.5 Gy.
9:15 AM Sorour Hosseini; Lianghao Ding; Delaney Felix; Elizabeth Polsdofer; Mary Wight-Carter;
Michael Story
(1648958) AVA AS A RADIATION COUNTERMEASURE AND SEX-BASED DIFFERENCES
IN RADIATION-DRIVEN LUNG CANCER
Evaluating the effects of radiation quality, sex disparities, and the potential for
pharmaceutical mitigation of radiogenic lung cancer risk with superoxide dismutase mimetic
GC4419
9:30 AM Honglu Wu; Kristyn Hoffman; Stephanie Krieger; Christopher Ngo
(1647150) COMPARISON OF RADIATION-INDUCED DAMAGE BETWEEN LIVERS FROM
CONTROL AND CHIMERIC MICE
Chimeric mice were exposed to gamma rays to investigate DNA damage and transcriptomics
changes in the humanized livers
9:45 AM Santosh Kumar; Elaina Kwiatkowski; Shubhankar Suman; Bhaskar Kallakury; Kamal Datta;
Albert Fornace
(1648205) EXPRESSION ANALYSIS OF STEM CELL MARKERS IN LOW-DOSE HIGH-LET
RADIATION INDUCED TUMOR IN APC1638N/+ MOUSE INTESTINE
High-LET radiation leads to more intestinal carcinomas compared to low-LET. In high-LET
group, adenomas have increased stem cell markers and β-catenin expression. This
heightened ISC marker expression in benign adenomas likely fuels their progression to
malignant tumors, evident in the higher carcinoma frequency.
10:00 AM Michael Weil; Justin Bell; Alexander Brandl; Lianghao Ding; Michael Story
(1646649) NASA SPECIALIZED CENTER OF RESEARCH ON CARCINOGENESIS
The Carcinogenesis NSCOR encompasses sequencing murine radiation associated and
sporadic tumors and source replacement in a neutron irradiator/vivarium facility.
10:15 AM Discussion
10:30 AM Break

495
Miniature Marvels: Exploring Tissue Chips and Organoids for Use in Space Health Research
9:00 AM Galleon
Chairs: Lynn Harrison and Kristin Fabre

9:00 AM Katherine Wozniak; Victoria Poplaski; Sarah Blutt; Robert Britton
(1644914) RSPO3 MITIGATES SPACE RADIATION DAMAGE IN HUMAN INTESTINAL
ORGANOIDS
We are currently testing L. reuteri-secreted variants of the RSPO3 potentiator on irradiated
differentiated and undifferentiated organoids to understand the cell biological response to
radiation.
9:12 AM Sivan Osenberg; Lindsay Altidor; Lawrence Bronk; Hu Chen; Johnathan Dexuan Jia; David
Grosshans; Fada Guan; Anel LaGrone; Zhandong Liu; Mirjana Maletic-Savatic; Luke
Parkitny; Alexander Trostle; Mostafa Waleed Gaber; Ying-Wooi Wan; Damian Young
(1650773) HUMAN BRAIN ORGANOID MODEL FOR STUDYING THE EFFECTS OF
SPACE RADIATION ON NEUROGENESIS
The generation of new neurons from neural stem cells is essential for formation of new
memories and mood control, and is highly sensitive to all forms of radiation. Here, we
examined the effects of space-like radiation on neurogenesis in human brain organoids. We
found increased apoptosis, decreased cell proliferation, and widespread changes in the
transcriptome and metabolome.
9:24 AM Piyumi Wijesekara; Katarina Lile; Hansjorg Schwertz; Sylvain Costes; Egle Cekanaviciute
(1650873) HUMAN AIRWAY RESPONSES TO SIMULATED SPACEFLIGHT STRESSORS
Investigating the effects of spaceflight stressors on the human respiratory system
9:36 AM Eun Ra; Gabsang Lee
(1647257) OPTOGENETIC STIMULATION OF FGF SIGNALING MITIGATES CELLULAR
DEFECTS INDUCED BY GALACTIC COSMIC RAY-LIKE RADIATION IN HUMAN BRAIN
ORGANOIDS
We introduced an innovative approach by employing optogenetically-regulated FGF2
signaling (opto-FGFR) to ameliorate the detrimental effects of SimGCRsim exposure on
brain organoids. Remarkably, opto-FGFR-brain organoids exhibited a significant rescue of
neural damage induced by GCR, achieved through the activation of downstream FGF2
signaling.
9:48 AM Daniel Tavakol; Ilaria Baldassarri; Roberta Lock; Ivana Matkovic; Francois Chesnais; Trevor
Nash; Keith Yeager; Diogo Teles; Connie Chen; Youngbin Kim; Pamela Graney; Maria
Samaritano; Derek Ning; Griffin Daly; Sally Amundson; Guy Garty; Kam Leong; David
Brenner; Gordana Vunjak-Novakovic
(1649609) ASTRONAUT-ON-A-CHIP: INDIVIDUALIZED HUMAN MULTI-ORGAN
PLATFORM FOR ASSESSING EFFECTS OF SIMULATED GALACTIC COSMIC
RADIATION
In this work, we developed a bioengineered model of multi-organ interactions in an
individualized, astronaut-specific context and investigated the effects of simulated galactic
cosmic radiation.
10:00 AM Adam Chicco; Xu Cao; Dilip Thomas; Luke Whitcomb; Claudia Wiese; Alex Brandl; Anushree
Chatterjee; Joseph Wu; Michael Weil
(1649510) EFFECTS OF CHRONIC HIGH LET RADIATION ON THE HUMAN HEART
Engineered human heart tissues and fibroblasts were irradiated at a low dose rate in the
presence and absence of a mitochondria-targeted antioxidant countermeasure to determine
functional impacts and identify biomarkers of space radiation injury on the human heart
10:12 AM Discussion
10:30 AM Break
505
Artificial Intelligence Supporting Exploration Spaceflight Health & Wellness
Chair: Michael Krihak

9:00 AM William Buras; Dylan Hashem; David Hilmers; Kyle Nguyen
(1649610) DEVELOPMENT OF SPACE MEDCINE GPT (S-MED GPT) FOR DIALOGUE-
BASED JUST-IN-TIME CLINICAL DECISION SUPPORT FOR CREW MEDICAL
AUTONOMY LONG DURATION SPACEFLIGHT MISSIONS
We are building a Large Language Model (LLM) GPT (Generative Pre-Trained Transformer)
Knowledge Base, trained with information from large medical databases, including additional
information derived from space medicine libraries, to be an innovative, and multi-role
crewmember (CMO and non-physician) tool for spaceflight Clinical Decision Support and
medical procedural guidance.
9:20 AM Jayant Menon; Audrey Aldridge; Jason Kwan; Michael Novitzky; Rob Semmens
(1649626) PRE-CLINICAL EVALUATION OF AN AUTONOMOUS MEDICAL RESPONSE
AGENT (AMRA) WITH ASTRONAUT ANALOG USERS IN SIMULATED PROLONGED
FIELD CARE SCENARIOS AT THE UNITED STATES MILITARY ACADEMY WEST POINT
Results of a prospective randomized pre-clinical experimental evaluation of the Autonomous
Medical Response Agent Software will be presented. US military Academy Cadets served as
non-expert astronaut analogue users treating four different simulated space medical
scenarios. Astronauts responded to these medical scenarios as if they were on a deep space
mission.
9:40 AM Victoria Hurd; Michael Del Valle; Victoria Kravets; Arian Anderson; Allison Anderson;
Matthew Riscinti
(1647728) SKILL RETENTION USING AI-ASSISTED POINT-OF-CARE ULTRASOUND IN
NOVICE, TECHNICALLY COMPETENT USERS
Point-of-care ultrasound artificial intelligence tools impact skill degradation rates in astronaut-
representative subject pools, as subjects with AI tools correctly captured more bladders in
their scans and perceived the scanning setup to be more usable over a 2 month span.
10:00 AM Neil McDonald; Ryan Dunnam; Kristy Hollingshead; Melissa Poquette; Anil Raj; Walid
Soussou
(1677679) INDIVIDUALIZED, NONINVASIVE SPEECH INDICATORS FOR TRACKING
ELEVATIONS IN STRESS (INSITES)
This NASA-funded SBIR project is a collaboration between QUASAR and IHMC aimed at
addressing the need of crew monitoring by developing a system to identify Individualized,
Noninvasive Speech Indicators for Tracking Elevations in Stress (INSITES) with the overall
goal of developing an unobtrusive, objective, and reliable detector of stress that measures
changes in speech and vocalizations
10:20 AM Discussion
10:30 AM Break

February 13-16, 2024 515
Behavioral Health and Performance Over Time: Results from Ground Analogs and Spaceflight
Chairs: Nick Kanas and Jayati Roy Choudhury

9:00 AM Nick Kanas
(1630256) DO TIME-DEPENDENT EMOTIONAL STAGES OCCUR IN LONG DURATION
SPACE MISSIONS?
This presentation examines the evidence for time-dependent emotional stages (like the third
quarter phenomenon) in long duration space missions and concludes that their presence has
not been supported as universally occurring and that emotional reactions during lunar and
Mars expeditions may be better explained by specific mission events.
9:13 AM Sheena Dev; Lauren Landon; Steven Anderson; Jennifer Miller; Sydney Begerowksi;
Suzanne Bell
(1642809) THE HFBP-EM HARMONIZED DATASET: COMPARING BEHAVIORAL
MEDICINE AND TEAMS RISK BETWEEN SHORT AND LONG DURATION SPACEFLIGHT
ANALOGS
In this presentation, we will utilize an updated harmonized dataset that includes a larger
sample and longer mission durations to compare the trajectory of behavioral medicine and
teams-based measures between long and short mission durations.
9:26 AM Christopher Jones; Christopher Jones; Mark Kaizi-Lutu; Alex Stahn; Gary Strangman;
Vladimir Ivkovic; Warren Bilker; Jack Stuster; Adrian Ecker; David Dinges
(1649706) DATA HARMONIZATION OF NASA BEHAVIORAL HEALTH AND
PERFORMANCE (BHP) STANDARDIZED BEHAVIORAL MEASURES TO IDENTIFY
BEHAVIORAL RESPONSES ACROSS SPACEFLIGHT ANALOG MISSIONS
This abstract presents the findings from the NASA Behavioral Core Measures data
harmonization supplement.
9:39 AM Vladimir Ivkovic; Stijn Thoolen; Bryan White; Quan Zhang; Gary Strangman
(1650739) OPERATIONAL PERFORMANCE MEASURES:
EFFECTS OF ISOLATION AND CONFINEMENT IN HERA RELATIVE TO NON-ISOLATED
CONTROLS
HERA missions of 45-day duration adversely affected both learning and performance of the
operationally-relevant ROBoT-r task over the first two weeks of isolation and confinement
relative to performance by the non-isolated control group.
9:52 AM Gary Strangman; Vladimir Ivkovic
(1649499) ROBOTIC PERFORMANCE FOR MARS LANDINGS: ROBOT-R ON R+0
To support the Mars Capability team, we used the operationally relevant ROBoT-r simulator
to identify any significant performance changes in long-duration ISS cremembers
immediately after landing (on R+0) compared to in-flight performance.
10:05 AM Ashley Weaver; Diana Madrid
(1646865) QUANTIFYING CHANGES IN NECK MUSCLE HEALTH FOLLOWING
SPACEFLIGHT VIA MRI
Neck muscle size and composition were assessed in ISS astronauts from pre-flight to post-
flight MRI scans.
10:18 AM Discussion
10:30 AM Break

February 13-16, 2024 525
SANS 2: Bed Rest
Chairs: Brandon Macias and Steve Laurie

9:00 AM Brandon Macias
(1647349) MECHANICAL AND GRAVITATIONAL COUNTERMEASURES TO OCULAR
CHANGES DURING STRICT HEAD-DOWN TILT BEDREST: THE SANS
COUNTERMEASURE BEDREST STUDY
The goal of this study is to determine whether 6 hours of daily fluid shift reversal with 25
mmHg lower body negative pressure (LBNP), upright seated posture, or 6 hours of daily use
of veno-constrictive thigh cuffs (VTC) following 1 hour of aerobic exercise can reverse the
headward fluid shift and prevent development of SANS findings, as compared to HDT
controls.
9:15 AM Alex Huang; Rustum Karanjia; Ye He; Steven Laurie; Matthew Poczatek; Stuart Lee;
Millennia Young; Ken Marion; Srinivas Sadda; Brandon Macias
(1639731) EXERCISE AND LOWER EXTREMITY THIGH CUFF COUNTERMEASURES TO
OCULAR CHANGES DURING STRICT HEAD-DOWN TILT BEDREST: THE SANS
COUNTERMEASURE BEDREST STUDY
The Spaceflight Associated Neuro-ocular Syndrome Bed Rest Countermeasure Study.
9:30 AM Bryn Martin; Khader Hasan; Larry Kramer; Steven Laurie; Brandon Macias; Stuart Sater;
Katherine Warthen; Michael Williams
(1646485) OPHTHALMIC AND INTRACRANIAL STRUCTURAL CHANGES IN HEAD-
DOWN TILT BED REST (OPTICS): UPDATE ON FINDINGS AND COMPARISON TO
ASTRONAUTS
The aims of the OPTICS study are to 1) quantify the impact of HDT on the eye, brain, and
CSF system, 2) determine if countermeasures can prevent changes to the eye, brain, and
CSF system over HDT, 3) compare alterations in HDT to astronauts that developed SANS,
and 4) confirm measurement precision in controls.
9:45 AM* Scott Smith; Alice Chang; John Chen; C Robert Gibson; Jesse Gregory; Thomas Mader;
Steven Zeisel; Sara Zwart
(1642853) ONE-CARBON METABOLISM AND SANS: 2024 UPDATE
An update will be presented on the status of the line of research investigating the role of the
genetics of one carbon metbolism and SANS.
9:45 AM* Sara Zwart; John Chen; Sarah Egert; Martina Heer; Steve Laurie; Brandon Macias; Scott
Smith; Patrick Stover
(1643403) SANS COUNTERMEASURE STUDY: ONE-CARBON METABOLISM AND
GENETICS
Findings from this bed rest study will further our understanding of the role of one-carbon
metabolism in at risk subjects and will dovetail with our ongoing test of the countermeasure
on ISS.
9:45 AM* Sara Zwart; Eric Bershad; Steve Laurie; Brandon Macias; Mark Melin; Scott Smith; Millennia
Young
(1643408) EXPLORING ENDOTHELIAL FUNCTION RISK FACTORS AND OPTIC DISC
EDEMA DURING STRICT 6º HEAD-DOWN TILT BED REST
Baseline optic cup volume and genetics together provided a better model of predicting the
change in total retinal thickness during a 60-d head-down tilt bed rest study, than did
genetics alone.

February 13-16, 2024 535
9:45 AM* Sara Zwart; John Chen; Martina Heer; Steve Laurie; Brandon Macias; Cambria O'Grady;
Scott Smith; Patrick Stover
(1643410) B COMPLEX: A NUTRACEUTICAL SANS COUNTERMEASURE
The primary aim of this study is to provide a nutraceutical countermeasure for SANS, and to
assess effectiveness in at risk individuals based on their genetic profiles.
10:00 AM Stijn Thoolen; Quan Zhang; Vladimir Ivkovic; Susan Voss; Stefan Moestl; Timo Frett; Jens
Tank; Jimmy Wu; Eric Bershad; Gary Strangman
(1648464) BRAIN-SANS: BRAIN-RELATED ASSESSMENTS FOR INVESTIGATING THE
NEUROPHYSIOLOGY OF SANS - 2024 UPDATE
At the DLR’s :envihab facility, we are investigating the neurophysiological changes
associated with 30-day, 6 degrees head-down tilt bedrest—with and without
countermeasures—as part of the SANS-CM campaigns.
10:15 AM Discussion
10:30 AM Break

February 13-16, 2024 545
Space Radiation Cross Risk Integrations Projects Introduction
Chairs: Janapriya Saha and Corey Theriot

1:05 PM Corey Theriot; Christian Castro; Cruz Torres; Greg Eley
(1646605) NASA OMICS ARCHIVE FLIGHT STUDY UPDATE
As a update, we review details regarding the biospecimen and data archive to be generated
by the NASA Omics Archive Flight Study.
1:20 PM Hari Ilangovan; Greg Eley; Robin Elgart; Parastou Eslami; Katherine Hoadley; Xiao Wen
Mao
(1646902) MULTI-OMICS STUDY OF THE EFFECT OF REDOX-ACTIVE
METALLOPORPHYRIN ON MURINE RETINA DURING SPACEFLIGHT
Astronauts returning from spaceflight have experienced eye problems, which may decrease
retinal performance and lead to long-term effects on visual acuity. This study leverages multi-
omics data from spaceflown murine retinas that were treated with redox-active
metalloporphyrin (BuOE) to mitigate spaceflight-induced changes and respective ground
controls.
1:35 PM Keith Siew; Stephen Walsh
(1665956) COSMIC KIDNEY DISEASE: A PAN-OMIC INVESTIGATION OF THE HEALTH
CONSEQUENCES OF A TRIP TO MARS AND BACK
Largest study to date of microgravity & galactic cosmic radiation impacts on kidney health.
With data from 20 missions combining multiple species, modalities & tissue types, we report
space-induced changes in protein post-translational modifications governing renal electrolyte
homeostasis, evidence of structural remodelling of kidney nephrons, early signs of
dysfunction & irreversible organ damage
1:50 PM Xu Cao
(1649137) IDENTIFYING GENETIC FACTORS IN RADIATION INJURY WITH POOLED
SINGLE CELL SEQUENCING
This study aim to investigate inheritable/genetic factors that may affect people’s susceptibility
to space radiation , which can help us better predicting the disease risk and design
personalized drugs.
2:05 PM Neal Lojek; Victoria Williams; Andrew Rogers; Erno Sajo; Bryan Black; Chiara Ghezzi
(1627444) A 3D IN VITRO CORTICAL TISSUE MODEL FOR STUDYING EFFECTS OF
IONIZING RADIATION ON HUMAN NEURONAL NETWORK FUNCTION
A novel 3D in vitro human cell-based cortical tissue model (CTM) that (1) recapitulates tissue
mechanical properties, (2) supports cortical cell types, (3) is amenable to ionizing radiation
treatment workflows, (4) and enables high-content phenotypic assessment of neuronal
network function.
2:15 PM Megan Waldock; Kim Ayers; Rory Curtis
(1647675) WEARABLES FOR DERMAL BIOINDICATORS - YET2 TECHNICAL SURVEY
yet2, a technology scouting and open innovation consulting company, began working with
NASA HRP in September 2023 to conduct a landscape analysis of partners/technologies for
solutions that can monitor dermal bioindicators.
2:30 PM Break

February 13-16, 2024 555
Joint Panel on the Role of the Vestibular Organs in Space Exploration
1:00 PM Galleon
Chairs: Allie Anderson and Ana Diaz Artiles

1:05 PM Scott Wood; Nichole Beltran; Austin Bollinger; Tim Macaulay; Vernie Daniels; Zuwei Wang
(1650804) EVALUATION OF INTRANASAL SCOPOLAMINE FOR THE PREVENTION OF
WAVE MOTION INDUCED MOTION SICKNESS WHILE MAINTAINING PERFORMANCE
ON OPERATIONALLY RELEVANT TASKS
Our study goal is to characterize the effectiveness of motion sickness countermeasures such
as the intranasal scopolamine during controlled laboratory experiments using capsule wave
motion simulation and during field testing in operational environments.
1:20 PM David Temple; Talie Stone; Torin Clark
(1645025) ENHANCEMENT OF VESTIBULAR PERCEPTUAL THRESHOLDS WITH NOISY
GALVANIC VESTIBULAR STIMULATION: IMPLICATIONS AS A SENSORIMOTOR
COUNTERMEASURE
We are investigating the use of noisy galvanic vestibular stimulation to enhance vestibular
performance via a phenomenon known as stochastic resonance, for potential use as a
gravity transition sensorimotor countermeasure.
1:35 PM Vivekanand Vimal; Paul DiZio; James Lackner
(1649297) VIBROTACTILE FEEDBACK AS A COUNTERMEASURE FOR SPATIAL
DISORIENTATION IN EARTH-G, MARTIAN-G, LUNAR-G AND 0-G ANALOGS
In this project we examined how different levels of gravitational cues influenced the
magnitude of spatial disorientation, the ability to stabilize a vehicle and the effectiveness of
vibrotactile feedback as a countermeasure for spatial disorientation.
1:50 PM Mark Shelhamer; Nabila Ali; Jennifer Law; Jaime Mateus; Anil Menon; Mallika Sarma;
Michael Schubert; Serena Tang; Emmanuel Urquieta; Victor Yang
(1638389) OTOLITH AND POSTURE EVALUATION IN COMMERCIAL SPACEFLIGHTS
Data on ocular alignment and postural control from preflight/postflight testing on commercial
spaceflights reveal possible predictive testing for SMS based on otolith asymmetry, and may
link asymmetry to postural sway after flight.
2:05 PM Catho Schoenmaekers; Dmitrii Glukhikh; Steven Jillings; Ilya Rukavishnikov; Elena
Tomilovskaya; Floris L. Wuyts; Peter zu Eulenburg
(1649337) NEURAL CORRELATES OF VESTIBULAR ADAPTATION IN COSMONAUTS
AFTER LONG DURATION SPACEFLIGHT
We have previously investigated the otolith-mediated ocular counter-roll (OCR) where we
found a difference in eye torsion pre- to postflight. The current study aimed to investigate
retrospectively if OCR changes pre- to postflight correlate with functional connectivity
changes in specific vestibular cortical regions after a long duration mission to the
International Space Station (ISS).
2:20 PM Discussion
2:30 PM Break

February 13-16, 2024 565
Point of Care Testing Supporting Exploration Spaceflight Health
Chair: Chris Zahner

1:00 PM Alexandru Mezin; Courtney Schkurko; Rahul Suresh; Moriah Thompson; Kimesha Calaway;
Brian Hromco; Alexander Salimian; Tyler Hatch
(1637539) ISS INTRAVENOUS FLUID GENERATION MINIATURIZATION (IVGEN MINI)
FOR EXPLORATION MISSIONS
The IVGen Mini design builds upon the success and lessons learned of the original IVGEN
project with regards to production of medical grade IV fluids from the potable water supply of
an exploration vehicle or habitat and would reduce medical risk and the mass and volume
footprint of the medical system.
1:15 PM Rachael Miller; Beth Lewandowski; Courtney Schkurko; Kimesha Calaway; Yayu Hew;
Alejandro Garbino; Emily Stratton; Moriah Thompson; Rahul Suresh; Justin Yang; Russell
Valentine; Kris Lehnhardt; Benjamin Easter
(1645538) EXPLORATION ATMOSPHERE DEMONSTRATION OF A MULTI-FUNCTIONAL
INTEGRATED MEDICAL DEVICE (TEMPUS PRO)
Usability of an integrated vital signs and medical imaging device, Tempus Pro, was
demonstrated in 34% oxygen at 8.2psi in conjunction with the Exploration Atmospheres-2
(EA-2) study in the 20-Foot Exploration Atmosphere Chamber at Johnson Space Center.
1:30 PM Mimi Lan; Darin Knaus; Clive Devoy; Kate Fergusson; Scott Phillips; Samantha Leigh;
Shireen Geimer; Jay Buckey
(1649558) ULTRA-COMPACT URINARY CALCIUM MEASUREMENT DEVICE URINE
TESTING RESULTS
This abstract reports on urine testing results of a novel device to test urinary calcium
concentrations in spaceflight for kidney stone risk mitigation and as a marker of bone health.
1:45 PM Roddy MacCallum; Hayden Hodges; Megan Waldock
(1647581) COMPACT MEDICAL LAB - YET2 TECHNICAL SURVEY
From June 2023 to October 2023, yet2, a technology scouting and open innovation
consulting company, worked with NASA XMIPT to identify compact, point-of-care
technologies that could be used for medical diagnosis, monitoring, and treatment of medical
conditions based on clinical lab analysis of biological samples (e.g., blood and urine).
2:00 PM Hongyi Zhang; Megan Waldock
(1647607) COMPACT RADIOGRAPHY EQUIPMENT - YET2 TECHNICAL SURVEY
From June 2023 to October 2023, yet2, a technology scouting and open innovation
consulting company, worked with NASA XMIPT to identify compact radiography equipment
for x-rays imaging and analysis.
2:15 PM Discussion
2:30 PM Break

February 13-16, 2024 575
Behavioral Health and Performance in Isolation and Confinement: Outbrief from SIRIUS and HERA
Chairs: Suzanne Bell and Alexandra Whitmire

1:00 PM Suzanne Bell; Steven Anderson; Lauren Landon; Sheena Dev; Zach Glaros; Bruce Keller;
Erin Flynn-Evans
(1648282) HUMAN FACTORS AND BEHAVIORAL PERFORMANCE EXPLORATION
MEASURES IN NEK
We collected data on the Human Factors and Behavioral Performance Exploration Measures
suite from a 6-person crew in isolation for 240 days. We summarize key findings and results
from our aims related to the operational feasibility and acceptability of the measures, and the
behavioral health, team functioning, and performance of the crew over time.
1:15 PM Gloria Leon; Bruce Keller; Jennifer Miller; Suzanne Bell
(1645914) PERSONALITY, AFFECT AND COPING AMONG SIRIUS 21 CREW
Psychological evaluation of SIRIUS 21 crew members from pre-ingress to a period following
egress.
1:30 PM Ute Fischer; Kathleen Mosier; Shane Mueller; Elizabeth Veinott
(1648276) THE IMPACT OF CREW AUTONOMY ON THE SPACE/GROUND MULTI-TEAM
SYSTEM
This research explored the impact of crew autonomy on crewmembers’ and mission
controllers’ cognition and collaboration in 10 simulated space missions and observed that
crew autonomy may exacerbate fault lines of remote collaboration concerning important
aspects of team cognition, such as team cohesion and efficacy as well as a shared
understanding of team members' roles and responsibilities.
1:45 PM Shawn Burke; Andres Kaosaar; Krisztina Szabo
(1650122) TAKING A SIRIUS PERSPECTIVE: A TALE OF TWO ANALOG CREWS
Examines team interaction within two multicultural crews that participated in the SIRIUS
analog (4 and 8-month missions), with a specific focus on the role of culture on crew
interaction.
2:00 PM Alina Lungeanu; Leslie DeChurch; Noshir Contractor
(1644015) CROSSING BOUNDARIES IN SPACE EXPLORATION MULTITEAM SYSTEMS:
SIRIUS-21 INSIGHTS
This study investigates cooperation among the crew and mission support as autonomy
shifted during the SIRIUS-21 mission.
2:15 PM Discussion
2:30 PM Break

February 13-16, 2024 585
SANS 3: Other Models
Chairs: Brandon Macias and Steve Laurie

1:00 PM Rashika Rao; Sutton Richmond; Rayn Ramclam; Swati Rane; Dawn Kernagis; Kody
Coleman; Mehmet Albayram; Jens Rosenberg; Jeffrey Iliff; Rachael Seidler
(1649123) EFFECT OF HEAD-DOWN TILT +/- CO2 ON GLYMPHATIC FUNCTION
Impairment of the glymphatic system is thought to be a potential factor in SANS; we will use
head-down tilt and carbon dioxide breathing mixes as microgravity analogues to investigate
the effect of extreme environments on human glymphatic clearance.
1:13 PM Steven Jillings; Bjorn Baselet; Mieke Verslegers; Chrity Lodder; Roel Quintens; Mieke Neefs;
Jasmine Buset; Johan Van Audekerke; Nicholas Vidas-Guscic; Joëlle Van Rijswijk; Ben
Jeurissen; Marleen Verhoye; Annemie Van der Linden; Floris Wuyts
(1649959) A HINDLIMB UNLOADING STUDY IN MICE TO STUDY FLUID SHIFT AND
BRAIN STRUCTURAL CHANGES
Brain MRI data and immunohistochemistry in mice undergoing 14 days of hindlimb unloading
as a spaceflight analog to study brain structural changes and fluid shift.
1:26 PM* David Zawieja; Walter Cromer; Scott Zawieja; Olga Gasheva; Jorge Castorena Gonzalez;
Wei Wang; Katherine Kelly; Malea Murphy; Kyle Zawieja; Felicia Hunter; S Muthusamy; S.
Tsai; X Xu; W Xie; Robert Rosa; Y Ren; Scott Greenwald; Steven Laurie; Brandon Macias;
Ray Mitchell; Anatoliy Gashev; Pooneh Bagher; Binu Tharakan; Travis Hein
(1650112) EFFECTS OF SPACEFLIGHT ON MOUSE OCULAR VASCULAR
HYDRODYNAMICS AND IMPLICATIONS FOR SPACEFLIGHT ASSOCIATED NEURO-
OCULAR SYNDROME DEVELOPMENT
These ISS flight experiments in mice provide the first insights into the impact of
weightlessness/spaceflight on ocular vascular hydrodynamics and indicate potential
vasomotor alterations that may contribute to development of SANS in astronauts during
spaceflight
1:26 PM* O'lisa Waithe; Saravanakumar Muthusamy; Steven Laurie; Brandon Macias; David Zawieja;
Travis Hein; Binu Tharakan
(1650342) EFFECT OF SIMULATED MICROGRAVITY ON BLOOD-BRAIN AND BLOOD-
RETINAL BARRIER TIGHT JUNCTION PROTEINS IN A MOUSE MODEL OF HINDLIMB
UNLOADING
Investigating SANS with respect to BBB/BRB hyperpermeability in a hindlimb unloading
mouse model
1:49 PM Xiao Wen; Seta Stanbouly
(1660754) EFFECTS OF SPACEFLIGHT AND ARTIFICIAL GRAVITY ON THE MOUSE
RETINA: A PROGRESS REPORT
The purpose of the study is to determine the extent to which continuous partial gravity
provided by centrifugation on the International Space Station (ISS) as a countermeasure
mitigates the detrimental effects of microgravity on the retina.
2:02 PM Jay Buckey; Mimi Lan
(1649483) A RE-EVALUATION OF THE ACUTE EFFECTS OF WEIGHTLESSNESS AND
WHAT IT MEANS FOR SANS
The presentation will offer a re-evaluation of how to conceptualize the acute cardiovascular
effects of weightlessness and how this is relevant to SANS.
2:15 PM Discussion
2:30 PM Break
February 13-16, 2024 595
Poster Session B: Behavioral Medicine (BMed)

041 Namni Goel; Lauren Pasetes
(1607271) PHENOTYPIC STABILITY OF ACTIGRAPHIC SLEEP MEASURES ACROSS
LONG-DURATION INTERVALS INVOLVING REPEATED SLEEP DEPRIVATION AND
RECOVERY
The goal of this project is to determine how total sleep deprivation affects individual
differences in actigraphic sleep measures as well as the stability of these measures during
months 2 and 4 across the NEK 4-month and 8-month duration missions.
042 Eiman Jahangir; Bindhu Oommen; Mira Milas; Eric Petersen; Brandy Nunez; Jas Purewal
(1608332) PHYSIOLOGIC IMPACT FROM MISSION INDUCED STRESS AND IMPACT OF
AN ARTIFICIAL INTELLIGENT ROBOT SEAL DURING A TERRESTRIAL SPACE ANALOG
MISSION
This study aimed to understand the ability of a PARO therapeutic artificial intelligent robot
seal to reduce stress after during a terrestrial space analog mission.
043 Joshua Elston; Erin Richardson; Allison Anderson
(1609158) DIMENSIONALITY REDUCTION METHODS FOR MODELING OPERATOR
COGNITIVE STATES
To predict an individual's trust, workload, and situation awareness (TWSA) for deep-space
human-autonomy teaming applications, alternative dimensionality reduction methods must
be analyzed to define physiological-based model-building processes that achieve both
stability and high predictive performance.
045 Amelie Therre; Edwin Mulder; Stefan Möstl; Daniel Aeschbach; Christian Mühl
(1622219) EFFECTS OF LOWER BODY NEGATIVE PRESSURE AND EXERCISE DURING
30 DAYS OF STRICT HEAD-DOWN-TILT BED REST ON VISUAL SEARCH
PERFORMANCE AND ASSOCIATED OCULOMETRIC MEASURES
We investigated the effects of LBNP and exercise during simulated microgravity through bed
rest on oculomotor and cognitive performance in visual search tasks, finding that exercise
was most effective against detriments, indicating that it may not only have mitigated the
headward fluid shift but also prevented alterations in voluntary eye movements, potentially
enhancing visual search performance.
046 Afik Faerman; Jonathan Clark
(1634108) CAPTURING THE OVERVIEW EFFECT: A PROTOCOL DESIGN
We describe a protocol designed to explore the Overview Effect in commercial spaceflight
(data collection is planned to start in the next two years).
047 Gyutae Kim; Kyu-Sung Kim
(1637255) PROTON EXPOSURE INDUCED COGNITION-RELATED BEHAVIORAL
ALTERATION
Proton-induced effects was identified by the computed behavioral markers, and the analysis
helped to distinguish the effect by a low amount of proton from that of control.
048 Timon Ax; Bergita Ganse; Fabian Fries; Cintia De Paiva; Francesc March De Ribot; Slade
Jensen; Berthold Seitz; Thomas Millar
(1641324) DRY EYE DISEASE IN ASTRONAUTS
We reviewed the current literature on the prevalence and mechanisms of DED in astronauts
and its potential implications for long-duration spaceflight, including the influence of
environmental factors, such as microgravity and fluid shift on tear film physiology in space.

February 13-16, 2024 605
049 Lilian Felipe; Maryela Angeles; Andrea Galindo

(1642377) EVALUATING SPACE MOTION SICKNESS USING FUNCTIONAL NEAR-
INFRARED SPECTROSCOPY (FNIRS): A PROMISING APPROACH
Space motion sickness (SMS) is a challenge in space exploration, impacting astronauts' well-
being and performance. Traditional self-report assessments lack timeliness and accuracy.
This study underscores fNIRS' potential for objectively evaluating SMS, aiding
countermeasure development for astronauts.
050 Gilles Clement; Suzanne Bell; Christian Castro; Brian Crucian; Sheena Dev; Stephanie
Krieger; Stuart Lee; Brandon Macias; Sarah Moudy; Scott Smith; Corey Theriot; Sarah
Wallace; Scott Wood; Sara Zwart
(1643019) STANDARD MEASURES DURING SPACEFLIGHT
The goal of the Spaceflight Standard Measures project is to ensure that a set of measures,
representing the Human Research Program’s key risks and acquired with minimal impact on
time and resources, is consistently captured from crew members through the end of the
International Space Station Program. In this presentation, we will review the data collected to
date on 31 ISS crew members.
051 Kole Lutz; Sharon Hagle; Barret Schlegelmilch; Andres Permuy
(1643348) THE FIRST SPACE FOR YOUTH MISSION (S4Y) IN LOW EARTH ORBIT
Since the1960’s, youth have not been considered for space missions. The Space4Youth
(http://space4youth.net/) mission is proposed for 1-2+ days in 550-600km or suborbital with
RLV. With crowdfunding and cost sharing from gov and industry,four youth aged 15-18 would
be selected and trained. Biomedical analysis and DNA sequencing quantifies how youth
adapt to spaceflight associated symptoms and uG.
052 Francesco Pagnini; Francesco Pagnini; Stijn Thoolen; Nick Smith; Angelique Van Ombergen;
Francesca Grosso; Ellen Langer; Deborah Phillips
(1643497) MINDFULNESS IN ANTARCTICA: THE ROLE OF MINDFUL DISPOSITION IN
AN ISOLATED AND CONFINED ENVIRONMENT
Mindfulness disposition is a protective factor against stress in a winter-over isolated and
confined mission in Antarctica
053 Weiwei Zhou; Ki-Hun Hong; Wilsaan Joiner; Stephen Robinson; Jonathon Schofield
(1644157) EXAMINING THE TRANSFER OF OPERATIONAL SKILL BETWEEN
DIFFERENT ROBOTIC SYSTEMS
This research proposes to create an adaptive and validated robotics post-launch training
approach that will maximize the required crew expertise and thus improve the safety and
success of high-risk robotic operations.
054 Madison Diamond; Lucie Ráčková; Inès Butin; Stanislas Boyer; Alexis Paillet; Laure Boyer;
Emma Chabani
(1644695) EOS - END OF STRESS: AN IMMERSIVE STRESS COUNTERMEASURE FOR
OPERATIONAL FIELDS
The EOS – End of Stress project, conducted by Human design Group and Spaceship FR
(CNES/MEDES), was conducted to specifically assess the ÉOS concept as a reactive
countermeasure to workplace and space stressors, with preliminary results demonstrating a
reduction in subjective stress and an increase in positive mood after experiencing the guided
ÉOS relaxation session.
055 Renee Abbott; Suzanne Bell; Ana Diaz-Artiles
(1644909) PROTECTING ASTRONAUT BEHAVIORAL HEALTH USING NATURE
PRESENTED WITH MULTISENSORY VIRTUAL REALITY
Overview of research using virtual reality with nontraditional stimuli (scents, temperature,
wind) as s countermeasure to improve mood and cognitive performance in isolated, confined
environments

February 13-16, 2024 615
056 Bryan White; Guus Vorst; Stijn Thoolen; Vladimir Ivkovic; Quan Zhang; Gary Strangman
(1645489) BRAIN-STIM: EFFECTS OF TRANSCRANIAL ELECTRICAL STIMULATION ON
OPERATIONAL PERFORMANCE MEASURES (ROBOT-R) AND ASSOCIATED NEURAL
RESPONSES
We are investigating whether transcranial electrical stimulation can enhance performance on
an operationally relevant task and hence whether it may be a suitable approach to help
counteract post-landing performance deficits.
057 Alex Stahn; Suzanne Bell; Katharina Brauns; David Dinges; Frank Fischer; Anika Friedl-
Werner; Darius Gerlach; Ruben Gur; Vladimir Ivkovic; Bernd Johannes; Simone Kühn;
Christian Mühl; Sarah Piechowski; David Roalf; Gary Strangman; Mathias Basner
(1646048) MATRIKS-NEO: MARS ADAPTIVE TRAINING INTEGRATIVE KNOWLEDGE
SYSTEM TO IMPROVE OPERATIONAL PERFORMANCE AND ITS NEURAL BASIS FOR
SPACEFLIGHT
This poster summarizes the development of an autonomous and self-adaptive training
approach that does not rely on mission control to maintain and improve operational
performance as part of the VNSCOR MATRIKS.
058 Alex Stahn; Mathias Basner; Vladimir Ivkovic; Poppy Barsby; Stephane Besnard; Katharina
Brauns; Pierre Denise; David Dinges; Anika Friedl-Werner; Hanns-Christian Gunga; Tom
Hartley; Stefan Hetzer; Alexander Garthe; Noah Miller; Bernhard Riecke; Guillaume
Spielmann; Matthias Stangl; Gary Strangman; Thomas Wolbers; Simone Kühn
(1646086) CIPHER SPATIAL COGNITION: VISUO-SPATIAL ABILITIES AND THEIR
NEURAL BASIS DURING LONG-DURATION LOW-EARTH ORBIT MISSIONS
This project summarizes the current status of the CIPHER project Spatial Cognition.
059 Gaëlle Quarck; Pierre Denise
(1646112) SPACEMED: A NEW ERASMUS MUNDUS JOINT MASTER IN PHYSIOLOGY
AND MEDICINE OF HUMANS IN SPACE AND EXTREME ENVIRONMENTS
SpaceMed is a completely new and fully integrated Masters which offers a full-time two-year
120 ECTS MSc programme with European Mobility. The objective of this Master's degree is
to provide graduates with a high level of knowledge and competencies in physiology and
space medicine.
060 Golda Nguyen; Aleksandra Stankovic; Katya Arquilla
(1646282) AUTOMATED BEHAVIORAL HEALTH ASSESSMENT USING NATURAL
LANGUAGE PROCESSING: A CASE STUDY OF ASTRONAUT JOURNALS
This work leverages modern natural language processing (NLP) techniques to perform
automated classification of affect and behavioral health state from publicly available
astronaut journals. The constructed language model's performance is presented, and the
results may inform the development of future autonomous, real-time behavioral health
monitoring systems for deep space missions.
061 Corey Theriot; Suzanne Bell; Steven Anderson; Sheena Dev; Thomas Oswald; Scott Smith;
Sara Zwart; Gilles Clement
(1646511) STANDARD MEASURES IN ANALOGS OF SPACEFLIGHT
We will review available SMCCP data collected during previous isolation studies.
062 Hannah Larson; Leia Stirling
(1646549) EVALUATING THE EFFECT OF MOTION TRAJECTORIES ON TAKEOVER
DECISIONS IN SPACECRAFT AUTONOMOUS RENDEZVOUS AND DOCKING
MANEUVERS
Spacecraft autonomous docking maneuvers supervised by human monitors were analyzed,
in which motion planning factors of the autonomous spacecraft and intrinsic spatial
awareness capabilities of the human monitor were investigated as influencers of the
monitor's situation awareness which informed manual takeover decision-making.

February 13-16, 2024 625
063 Jennifer Miller; Maya FarrHenderson; Ian Robertson; Suzanne Bell; Lauren Landon
(1646822) HABITABILITY AND HUMAN FACTORS ASSESSMENT (ISHORT, SHAQ, AND
SHU)
A novel assessment suite has been developed to further aid the comparison and
complementary understanding of the habitability and human factors measures, which will
allow for efficient deployment of these measures in analogs and/or spaceflight in near-term
research as well as support well-being and performance through design.
064 Aaron Massachi; Harrison Bressler; Aaron Massachi; Ben Robitshek; Jack Tajkef
(1647096) PINACAL: PINEAL GLAND STIMULATOR AND REGULATOR
The present study investigates the effect of light therapy through an individually
programmable, and wearable light therapy device, United States patent, “Pinacal.”
065 Savannah Buchner; Allison Anderson; Abigail Rindfuss; Hanspeter Scaub; Jennifer Wood
(1647948) IMPACTS OF VISUALIZATION AND IMMERSIVENESS IN DISPLAY DESIGNS
FOR REMOTE SUPERVISION
Studying the effects of visualization and immersiveness (VR) for remote supervision during
satellite operations.
066 Nicole Rote; Torin Clark; Jacob Kintz
(1647952) APPROACHES FOR ADAPTING AUTONOMOUS SYSTEMS BASED ON
OPERATOR COGNITIVE STATES
To increase crew and habitat self-reliance during deep space missions, operator cognitive
states will be modeled and used to adapt an autonomous system's interactions with the
operator.
067 Clara Richard; Gabriel De La Torre; Margot Issertine
(1648165) NEURODEGENERATION IN SPACE: A REVIEW OF THE NEURONS AND
GLIAL CELLS ALTERATION PROCESSES
This literature review provides a nuanced exploration of the intricate relationship between
space environments and neurodegeneration with a particular focus on the processes of
alteration in neurons and glial cells after long-duration spaceflight.
068 Sara Romanella; Lucia Mencarelli; Kimia Seyedmadani; Steven Jillings; Ilya Rukavishnikov;
Elena Tomilovskaya; Giulia Sprugnoli; Simone Rossi; Floris Wuyts; Emiliano Santarnecchi
(1648192) OPTIMIZING TRANSCRANIAL MAGNETIC STIMULATION FOR SPACEFLIGHT
APPLICATIONS.
We discuss the investigation of using transcranial magnetic stimulation (TMS) to counteract
brain health risks during long-duration space missions, where TMS can be optimized based
on individual brain anatomy and spaceflight-related changes to potentially enhance cognitive
and visuomotor functions in astronauts.
069 Suzanne Bell; Steven Anderson; Lauren Landon; Sheena Dev; Erin Flynn-Evans; James
Garrett; Millennia Young; Alaa Khader; Princess Dickson; Alonso Vera
(1648262) HUMAN FACTORS BEHAVIORAL PERFORMANCE RISK
CHARACTERIZATION RESEARCH FOR ARTEMIS II
We describe progress on our Human Factors and Behavioral Performance Research for
Artemis II study.
070 Andronikki Gerohristodoulos
(1648545) VIRTUAL REALITY AND BINAURAL AUDIO: THE ANSWER TO VISUALLY
INDUCED MOTION SICKNESS AND SPACE ADAPTATION SYNDROME?
A consideration of immersive virtual reality and binaural audio as means to mediate
symptoms of Visually Induced Motion Sickness and Space Adaptation Syndrome.

February 13-16, 2024 635
071 Mathias Basner; Suzanne Bell; David Dinges; Adrian Ecker; Yoni Gilad; Ruben Gur; Vladimir
Ivkovic; Christopher Jones; Tyler Moore; David Roalf; Kosha Ruparel; Alexander Stahn
(1648820) TEMPORAL NATURE OF COGNITIVE AND VISUOSPATIAL BRAIN DOMAIN
CHANGES DURING LONG-DURATION LOW-EARTH ORBIT MISSIONS:
NEUROSTRUCTURAL AND COGNITIVE CHANGES (PROJECT A)
In this CIPHER project, astronauts and matched ground controls will perform NASA’s
Cognition test battery to identify changes in general cognitive performance across all mission
phases. They will also perform a subset of the 10 Cognition tests in the MRI once before and
twice after the mission to inform the neural basis of any spaceflight-associated changes in
Cognition performance.
072 Sofia Ibarra; Torin Clark; Kieran Smith
(1649259) THE ROLE OF COGNITIVE FUNCTIONS IN SITUATION AWARENESS IN
INDIVIDUALS WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER
Tasks that require a high level of Situation Awareness (SA) and mental resources may pose
a challenge for people with Attention Deficit Hyperactivity Disorder (ADHD). This study will
collect physiological data from subjects operating an aircraft simulation. A model will be built
to estimate SA. The study will capture key differences in SA between people with ADHD and
neurotypical population.
073 Shibajyoti Banerjee
(1649264) PREDICTING VESTIBULAR DISORIENTATION VD USING DATA-DRIVEN
TECHNIQUES
Vestibular disorientation (VD) can cause dizziness, nausea, and vomiting, and impair
performance and safety in aviation, spaceflight, and military operations. This study aims to
develop predictive models for VD using data-driven techniques on a large dataset of
vestibular function data. This study intends to develop tools for identifying VD, which could
lead to improved outcomes. (399 characters)
074 Talmo Pereira; Lauren Sanders; Ryan Scott; Liezl Maree
(1649437) AUTOMATED DEEP LEARNING FOR SPACEFLIGHT RODENT BEHAVIOR
QUANTIFICATION AND HEALTH PHENOTYPING
This project seeks to assess and refine technology for automated behavioral analysis using
markerless motion capture and behavior motif detection tools, by analyzing rodent body
language through video data from the International Space Station's Rodent Research
system, validating the findings with RR-1 mission data, and laying the foundation for potential
human application in future missions.
075 Deanna Kennedy; Osmar Neto; Madison Weinrich; Nathan Keller; Renee Abbott; Traver
Wright; Bonnie Dunbar; Ana Diaz-Artiles
(1649651) EMG-EMG WAVELET COHERENCE OF MUSCLE COUPLING DURING
BIMANUAL TASKS PERFORMED IN PARABOLIC FLIGHT
EMG-EMG Coherence was used as a tool to investigation the neurophysiological effects of
partial G-levels delivered via parabolic flight on bimanual coordination dynamics.
076 Nataliya Kosmyna; Bryan White; Aleksandra Stankovic; Stijn Thoolen; Vladimir Ivkovic;
Pattie Maes; Gary Strangman
(1650185) DEVELOPMENT OF A PERSONALIZED PERFORMANCE OPTIMIZATION
PLATFORM (P-POP) FOR LONG DURATION SPACEFLIGHT
This project is developing and testing a multi-modal system to optimize human performance
by continuously adapting the user’s local sensory environment based on a closed-loop,
brain-computer interface (BCI) design.
077 Nataliya Kosmyna; Daniel Hails; Eugene Hauptmann; Christopher Markus; Zoe Lee; Gun
Bolukbasi; Pattie Maes; Gary Strangman
(1650206) P-POP: USING WEARABLE BRAIN SENSING GLASSES DURING ZERO-G
FLIGHT FOR HYPERSCANNING: PRELIMINARY STUDY
In this work we present a portable, wireless brain sensing system in a form-factor of a pair of
glasses, which we used to record and analyze EEG data from 6 adult flyers of a parabolic
flight with 3 types of gravity: Lunar, Martian and Zero gravity.

February 13-16, 2024 645
078 Nataliya Kosmyna; Bryan Michael White; Aleksandra Stankovic; Stijn Thoolen; Vladimir
Ivkovic; Pattie Maes; Gary E. Strangman
(1650230) P-POP: DEPLOYING MUSIC AND AUDITORY STIMULATION FOR REAL-TIME
IMPROVEMENT OF PERFORMANCE, RELAXATION AND WELL-BEING USING
PHYSIOLOGICAL BRAIN SENSING
The goal of this work is to provide an empirically validated system including both hardware
and software for customized intervention via music using the common form-factor of
headphones with incorporated biosensing for brain activity measurements for the
optimization of performance and mental well-being.
079 Nataliya Kosmyna; Eugene Hauptmann
(1650263) COGNITIVE STATES PLATFORM: MEASURING AND PROMOTING
PRODUCTIVITY AND WELL- BEING IN REAL-TIME USING PHYSIOLOGICAL SENSING
AND CLOSED-LOOP FEEDBACK INTERVENTIONS
In this paper we presented CSP, a platform featuring an enhanced version of the Cognition
battery on a multitude of platforms, which supports users by providing them with real-time,
closed-loop feedback about their level of engagement, cognitive load, attention and fatigue
while performing a set of tasks.
080 Amelia Gagnon; Katya Arquilla
(1650327) UTILIZING FUNCTIONAL NEAR INFRARED SPECTROSCOPY (FNIRS) FOR
OBJECTIVE MENTAL WORKLOAD MONITORING IN PILOTS DURING FLIGHT
SIMULATIONS
This study investigates functional near infrared spectroscopy (fNIRS) in an operational
environment to objectively monitor mental workload in commercial pilots as they perform
simulated approach-to-landing at various difficulties
121 Vladimir Ivkovic; Mathias Basner; Alexander Stahn; Guillaume Spielmann; JoAnna Pollonais;
Quan Zhang; Gary Strangman
(1650530) TEMPORAL NATURE OF COGNITIVE AND VISUOSPATIAL BRAIN DOMAIN
CHANGES DURING LONG-DURATION LOW-EARTH ORBIT MISSIONS: BASELINES OF
SLEEP, COGNITIVE/OPERATIONAL PERFORMANCE, AND IMMUNE FUNCTION
(PROJECT C)
Using ROBoT-r, we will investigate the interrelationships between spaceflight mission
duration, sleep quality/quantity, immune function, stress, neurocognitive and operational
performance on the ISS.
122 Vladimir Ivkovic; James Ehlers; JoAnna Pollonais; Guillaume Spielmann; Stijn Thoolen;
Bryan White; Quan Zhang; Gary Strangman
(1650698) CIPHER-PNI: QUANTIFYING FACIAL DEFORMATION IN HUT/HDT FOR
ASSESSING PSYCHONEUROIMMUNOLOGICAL CONTRIBUTION TO RISK OF
ADVERSE COGNITIVE / BEHAVIORAL CONDITIONS IN SPACEFLIGHT - 2024 UPDATE
Using HUT/HDT analogs we will generate 3D models of facial deformation due to cephalad
fluid shifts and images of facial affective expressions. Psychoneuroimmunological effects of
isolation and confinement will be assessed by facial affect recognition, cognitive, and stress
and immune markers pre/during/post HERA missions.
123 Cara Spencer; Suzanne Bell; Sheena Dev; Alaa Khader
(1650843) DEVELOPMENT AND VALIDATION OF A DATA PROCESSING PIPELINE FOR
ANALOG ECG DATA
We developed a novel processing pipeline for heart rate data that increases research
efficiency and potentially allows for near real-time processing of data on autonomous
missions.
124 Alexandra de Carvalho; Phil Brady; Sylwia Kaduk
(1650847) EMOTIONS IN EXTREME ENVIRONMENTS
This study examines emotions and emotion regulation strategies in extreme environments.

February 13-16, 2024 655
125 Aleksandra Stankovic; Stijn Thoolen; John-Michael Watson; Eric Olsen; Howard Rodenberg;
Jay Buckey; Gary Strangman
(1650891) VR IN ICE: QUANTIFICATION OF RESPONSE TO VIRTUAL REALITY-BASED
SENSORY STIMULATION FOR RELAXATION AND STRESS RELIEF IN ICE
ENVIRONMENTS
This project aims to optimize virtual reality (VR) sensory presentation for behavioral health
support in isolated, confined, and extreme (ICE) environments.
126 Kati Pettersson; Nathan Feick; Terence Tyson; Patrick Cravalho; Leeland Stone; Erin Flynn-
Evans
(1650895) CHANGES IN PVT REACTION TIME DURING ONE NIGHT OF SLEEP
DEPRIVATION
In this study, we employed linear mixed models (LMM) to investigate hourly-measured 5-
minute PVT reaction times during one night of total sleep deprivation. Our goal was to
characterize the contribution of homeostatic sleep pressure (time awake) and circadian
phase (salivary melatonin levels) on the dynamics of PVT reaction time.
127 Elena Fomina; Vera Bakhtereva; Anna Burakova; Natalia Senatorova
(1658617) EFFICIENCY OF COUNTERMEASURES TO MONOTONY IN LONG-DURATION
SPACE-FLIGHT
When planning long space flights, including manned expeditions to Mars, it is necessary to
understand what countermeasures for the prevention of hypogravity disorders will most
effectively contribute to the preservation of crew performance and will allow to fight
monotony, which inevitably arises in ultra-long space flights.

February 13-16, 2024 665
Poster Session B: Cancer

341 Ianik Plante; Steve Blattnig; Janice Huff; Amit Khera; Mark Little; Ryan Norman; Zarana Patel
(1619427) INTEGRATION OF THE CARDIOVASCULAR RISK MODEL ASTRO-CHARM IN
THE NASA RADIATION RISK MODEL
TBD
367 Marjan Boerma; Ashley Nemec-Bakk; Vijayalakshmi Sridharan; Michael Weil
(1631223) GAMMA-TOCOTRIENOL AS A COUNTERMEASURE AGAINST HIGH-ENERGY
CHARGED PARTICLE-INDUCED CARCINOGENESIS, CARDIOVASCULAR DISEASE,
AND CENTRAL NERVOUS SYSTEM EFFECTS
This project has generated a P53 mutant mouse model on two different genetic backgrounds
that may be used for radiation carcinogenesis studies.
368 Sean Jackewicz; Ed Perkins; Wei-Hsiung Yang
(1642863) ECTOPIC EXPRESSION OF GENOME STABILIZERS FOR DEEP SPACE
SURVIVABILITY
We have developed a bioengineering approach using a synthetic chromosome platform to
control the production of therapeutic genes, specifically focusing on TP53 gene expression,
which could enhance genome stability during genome editing events and have potential
applications in precision medicine for space exploration.
369 Kathleen Leon Parada; Gregory Lawson; Lovleen Bajwa; Kevin Gramajo-Aponte; Samantha
Getze; Birendra Mishra; Polly Chang; Eleanor Blakely; Ulrike Luderer
(1643424) OVARIAN FOLLICLE DEPLETION, OXIDATIVE STRESS, AND INFLAMMATION
PRECEDES OVARIAN TUMOR DEVELOPMENT AFTER MIXED HEAVY ION
IRRADIATION
Ovarian follicle depletion, oxidative stress, and inflammation precedes ovarian tumor
development after mixed heavy ion irradiation. Silicon ion radiation is hazardous to the
ovarian follicle reserve.
370 Shubhankar Suman; Kamendra Kumar; Bo-Hyun Moon; Santosh Kumar; Jerry Angdisen;
Bhaskar Kallakury; Albert Fornace
(1643986) ABT-263 MITIGATES 28SI-INDUCED GASTROINTESTINAL TUMORIGENESIS
IN APC1638N/+ MICE THROUGH DAMPENING OF SASP AND ONCOGENIC SIGNALING
In this proof-of-concept study, we show that administration of ABT-263 (a senolytic agent)
after heavy-ion radiation exposure decreased the incidence of GI cancer in the mouse
intestine, which was accompanied by the elimination of IR-induced senescent/SASP
accumulation and the attenuation of pro-inflammatory and oncogenic markers at the tissue
and systemic levels.
371 Fay Abdul Ghani; Peng Huang; Cuiping Zhang; Abba Zubair
(1644277) TRANSIENT EGFR OVEREXPRESSION MAY PROTECT ASTRONAUTS FROM
COSMIC RADIATION DURING DEEP SPACE EXPLORATION
The transient overexpression of EGFR in mesenchymal stem cells may protect astronauts
from cosmic radiation during deep space exploration, and is a model for enhancing the
human body to tolerate and recover from radiation injury in long-term manned space travel.
372 Heather Doviak; Megan Evans; Francine Garrett-Bakelman; David Goukassian; Karen
Hirschi; Ariel Polizio; Ken Walsh
(1644823) SPACE RADIATION EXPOSURE AND RISK MEDIATED BY CLONAL
HEMATOPOIESIS
Space radiation accelerates clonal hematopoiesis, but this cause and effect relationship may
depend upon which hematopoietic stem and progenitor cell “driver gene” is mutated.

February 13-16, 2024 675
373 Kamendra Kumar; Jerry Angdisen; Kamal Datta; Albert Fornace; Shubhankar Suman
(1645470) ELEVATED SASP FACTORS AND REDUCED ANTIOXIDANT ENZYMES IN
THE SERUM OF GCR-EXPOSED MICE COINCIDE WITH INCREASED MAMMARY AND
GASTROINTESTINAL CANCER INCIDENCE
This study has implications for the development of GCR-induced cancer risk biomarkers that
may also have utility to monitor the efficacy of a chemopreventive agent.
374 Michel Lapointe; Taylor Laframboise; Jake Pirkkanen; Simon Lees; Marc Medonca; Sergio
Santa Maria; T.C. Tai; Sujeenthar Tharmalingam; Douglas Boreham; Christopher Thome
(1647005) UNRAVELING THE BIOLOGICAL IMPACTS OF COSMIC RADIATION:
INSIGHTS FROM THE REPAIR PROJECT
The REPAIR (Researching the Effects of the Presence and Absence of Ionizing Radiation)
project investigates the biological impacts of cosmic radiation exposure relevant to deep
space crewed spaceflight with a unique approach. Via shielding of earth-surface level cosmic
radiation by conducting biological experiments 2 km underground in SNOLAB.
389 Poorvi Subramanian; Natarajan Aravindan; Sheeja Aravindan; Sreenidhi Mohanvelu; Dinesh
Babu Somasundaram
(1647744) FUNCTION OF RD3 IN SPACE RADIATION INDUCED CARCINOGENESIS:
META - ANALYSIS BASED EVIDENCE
Retinal degeneration protein 3 as a potential contributor to space radiation induced
tumorigenesis.
390 Masayo Morishita; Eric di Luccio; Takaaki Hirotsu
(1649263) NON-INVASIVE NEMATODE-NOSE (N-NOSE) TEST FOR EARLY-CANCER
DETECTION: PRE-FLIGHT SCREENING, IN-FLIGHT MONITORING, AND
RETROSPECTIVE ANALYSIS FOR ASTRONAUTS
We developed the world's first pioneering MCED Nematode-NOSE (N-NOSE), a non-
invasive, highly sensitive, and affordable primary cancer screening test, which more than
500,000 people have taken so far in Japan and here we introduce a possible application of
N-NOSE to health management of astronauts.
391 Britta Langen
(1650760) THE IMPACT OF CHRONODISRUPTION ON SPACE RADIATION-INDUCED
GENE SIGNATURES
This synergy effort applies radiochronobiology to biomarker discovery for space radiation-
induced lymphoma in mice bridging Cancer and Sleep elements in NASA's Human Research
Roadmap and overcoming knowledge gaps in genome-wide expression analysis and
biodosimetry.
392 Jerry Shay; Krisha Luitel
(1663917) LUNG CANCER PROGRESSION USING SIMULATED GALACTIC COSMIC
RADIATION
33 beam GCRsim plus neutrons increases risk for cancer in a mouse model of lung cancer
393 Anthony Davis; Shih-Ya Wang; Huiming Lu; Michael Story
(1664545) EVALUATION OF THE CELLULAR AND MOLECULAR RESPONSES OF LUNG
AND LIVER CELLS DERIVED FROM IPSCS WITH VARYING INTRINSIC
RADIORESPONSE TO SPACE RADIATION
The working hypothesis is “intrinsic radioresponse influences the risk for radiation-induced
cancer, particularly cancers generated by space radiation exposure”.
394 Amy Kronenberg
(1666806) WHAT CAN MUTATIONS TELL US ABOUT RISK?
Mutations are a hallmark of cancer. Without mutations cancers will not develop. Engineered
mouse models can identify specific oncogenic changes under restricted conditions, but
damage tolerance and repair in the mouse differ from what happens in humans. Here, a
sensitive human mutation assay system is used to demonstrate a wide variety of mutagenic
changes at limiting low doses and dose-rates.

February 13-16, 2024 685
Poster Session B: CBS

128 Curran Varma; Amy Beierschmitt; Daniela Berdnik; Xianjun Dong; Cynthia Lemere; Roberta
Palmour; Tony Wyss-Coray; Tingting Zhao; Gustaf Bostrom; Eva Luo
(1644218) PLASMA AND CSF BIOMARKERS OF AGING AND COGNITIVE DECLINE IN
THE CARIBBEAN VERVET: A PROTEOMIC STUDY
A proteomic study in the CSF and plasma of Caribbean vervets to identify biomarkers of
aging and cognitive decline.
129 Viktor Stolc; Miloslav Karhanek; Friedemann Freund; Yuri Griko; David Loftus; Maurice
Ohayon
(1645327) SOMATIC MUTATION IN MICE ON THE INTERNATIONAL SPACE STATION
(ISS): GUANINE SUBSTITUTION SUGGESTS LINK TO CANCER RISK
We analyzed ISS-flown mice for somatic mutations using GeneLab data. ISS mice showed
higher somatic mutation rates, correlated with gene expression. Guanine substitutions
prevailed, indicating a potential link between space environment and cancer risk, beyond
radiation. Implications for astronaut health will be discussed.
130 Richard Britten; Karra Johnson; Ella Tamgue
(1646632) SIMILARITIES AND DIFFERENCES IN ATSET PERFORMANCE DECREMENTS
INDUCED BY GCR EXPOSURE IN NAÏVE AND PREVIOUSLY IRRADIATED RATS.
Cognitive (ATSET) decrements induced by low ( < 20cGy) of space radiation in radiation-
naive and previously irradiated rats will be presented.
131 Madeline Ewell; Tanya Shawney; Emily Burke; Isabel Yu; Brian Kangas; Charles Limoli;
Rajeev Desai
(1648960) EFFECTS OF SLEEP FRAGMENTATION ON PERIPHERAL LIPIDOMIC AND
METABOLOMIC BIOMARKERS
The overall goal of these studies was to determine the effects of 6-hr or 10-hr sleep
fragmentation across five consecutive days on lipidomic and metabolomic biomarkers in
mice.
132 Joshua Alwood; Erik Antonsen; Sheena Dev; Gregory Nelson; Robert Reynolds; Abdullah
Shahid
(1650429) DAG STUDIES FOR THE BEHAVIORAL MEDICINE RISK
We plan to use the outcomes from these proposed DAG studies to identify candidate animal
data that builds coherence across the body of literature, thereby strengthening the level of
evidence for the Behavioral Medicine Risk per HSRB criteria
133 Joshua Alwood; Abdullah Shahid; Stephanie Puukila; Amber Paul; Siddhita Mhatre; Janani
Iyer; Candice Tahimic; Lauren Sanders; Robert Reynolds; April Ronca
(1650496) INTEGRATED ANALYSIS FOR THE CBS VNSCOR STUDIES
Our modeling approach integrates data from recent studies investigating the effects of
exposure to simulated galactic cosmic rays (GCRsim) on acute and delayed species-typical
behavior in mice (Puukila, et al., 2023) and immune/endocrine effects (Burke et al., in press).
134 Sanghee Yun; Amishi Mahajan; Grace Bancroft; Harley Haas; Elise Wallen-Friedman;
Shubha Vasisht; Hajime Takano; Frederico Kiffer; Amelia Eisch
(1694950) REAL-TIME MEASUREMENT OF HIPPOCAMPAL DENTATE GYRUS ACTIVITY
DURING PATTERN SEPARATION TESTING IN MALE MICE EXPOSED TO 33-PARTICLE
GALACTIC COSMIC RADIATION
Our study is the first to simultaneously measure hippocampal dentate gyrus (DG)-dependent
cognitive behavior and DG granular cell activity in vivo post-IRR, and they lay the
groundwork for delineation of how the brain responds to 33-beam galactic cosmic ray and
how those brain adaptations result in altered behavioral pattern separation.

February 13-16, 2024 695
135 James Brown; Ana Mora; Ishan Bansal; Elijah Hoffman; Robert Gunier; Stephen Rauch;
Tore Straume; Bernard Rabin; Richard Britten; Andrew Wyrobek
(1729183) DOSE-RESPONSE IS MAXIMAL AT OR BELOW 1CGY FOR DIVERSE SPACE-
ION EXPOSURES AND BEHAVIORAL/EXECUTIVE FUNCTION ENDPOINTS
Abstract Summary: We find that, depending on behavioral endpoints, single-ion exposures
can exhibit near-linear or threshold-like behavior with maximal relative effect (slope) at the
some of the lowest measure doses (1 cGy or below). At higher doses, we observe strong
evidence for non-monotonicity in response to exposures.

February 13-16, 2024 705
Poster Session B: CIPHER

281 Mary Bouxsein; Brett Allaire; Elisabeth Spector; Greg Yardley; Jean Sibonga
(1641967) MUSCULOSKELETAL ADAPATION AND RECOVERY TO LONG-DURATION
SPACEFLIGHT: THE CIPHER STUDY
This abstract describes the methods and progress for the Musculoskeletal Adaptations sub-
project of the CIPHER study.
282 Siddharth Chauhan; Matt Budoff; Michael Bungo; Katarina Ehrenborg; Srikanth Koneru;
Benjamin Levine; Jonathan Linder; Brandon Macias; David Martin
(1648735) CORONARY ANATOMY AND PHYSIOLOGY DURING 1 YEAR IN SPACE
[A.K.A. CORONARY]
This is a showcase of our ongoing cardiovascular investigation to coronary anatomy and
cardiac structural changes pre, during and post-flight by utilizing multiple cardiac imaging
modalities such as echocardiography, vasodilator stress/rest myocardial imaging, magnetic
resonance imaging and cardiac computed tomography to assess alterations through
spaceflight.
283 Gwyneth Gordon; Scott Smith; Sara Zwart; Ariel Anbar
(1659859) CIPHER BONE HEALTH: DYNAMIC NET BONE MINERAL BALANCE DURING
2-, 6- AND 12-MONTH MISSIONS ABOARD THE ISS
Using naturally occurring calcium isotopes in blood and urine, we 1) determine the time
course of net whole-body bone mineral balance in ten astronauts each during 2-, 6-, and 12-
month missions aboard ISS, 2) examine interpersonal variation in net bone loss/gain, and 3)
explore predictors for individuals who will experience above or below average rates of net
bone loss

February 13-16, 2024 715
Poster Session B: Dynamic Loads

136 Thomas Jeong; Karan Devane; Mitesh Lalwala; Jenna Miller; Ashley Weaver
(1642814) EFFECTS OF LONG-DURATION EXPOSURE TO MICROGRAVITY ON
VERTEBRAL STRENGTH
Identifying differences in vertebral injury risk due to long-duration microgravity exposure
through use of subject-specific finite element models and simulations.
137 Nathaniel Newby; Preston Greenhalgh; Teresa Reiber; Jeffrey Somers
(1646203) SOYUZ LANDING RISK CHARACTERIZATION
This study catalogs injuries due to dynamic phases of flight in the Soyuz vehicle, and seeks
to understand underlying mechanisms.
138 Sean Blade; Jenna Miller; Thomas Jeong; Karan Devane; Ashley Weaver
(1646667) IMPACT OF MICROGRAVITY ENVIRONMENT ON ASTRONAUT VERTEBRAL
COMPRESSIVE STRENGTH
Analysis of compressive strength of vertebrae using bone mineral density and subject
specific vertebral morphology for astronauts who spent 6+ months in microgravity
environment.
139 Karan Devane; Mitesh Lalwala; Bharath Koya; Max Chen; Scott Gayzik; Joel Stitzel; Ashley
Weaver
(1646871) COMPARING BODY KINEMATICS AND INJURY RISKS FOR FEMALE AND
MALE ASTRONAUTS IN A STANDING POSTURE DURING LUNAR LANDING/LAUNCH
Simulating lunar launch and landing events to quantify the differences in body kinematics
and injury risks for female and male astronauts using finite element human body models.
140 Han Kim; Pouyan Sabahi; Linh Vu; Sudhakar Rajulu
(1652318) 3D SCANNING SYSTEM TO ASSESS GRAVITY-DEPENDENT BODY SHAPE
CHANGES
The human body shows unique physiological and morphological changes when exposed to
different gravity conditions, including muscle atrophy, fluid shift, spinal elongation, and body
posture adjustments. This work aims to develop a prototype 3-D body scanning system that
is customized for in-flight use to scan crewmembers and optimized for detecting gravity-
dependent body shape and posture changes.

February 13-16, 2024 725
Poster Session B: Human Systems Integration Architecture (HSIA)

211 Eric Du; Tianyu Zhou
(1620916) SENSORY MANIPULATION AS A COUNTERMEASURE TO ROBOT
TELEOPERATION DELAYS
It reports findings of a sensory manipulation experiment with 94 subjects across various time
delays (250ms-5000ms) in robot teleoperation, i.e., haptic cues simulated via physics
engines could reduce task time, cognitive load, and enhanced self-confidence, while 18% of
subjects perceived smaller visual delays with haptic feedback, and efficacy waned for delays
beyond 1250ms.
212 Celia Avila-Rauch; Gabriel Gonzalez de la Torre
(1634708) PSYCHOLOGICAL ADJUSTMENT AND HUMAN FACTORS AT SPACE
ANALOG MISSIONS
Our brain has two decision-making systems: Rational and Emotional. Cognitive and
emotional processes in high-performance teams require a high level of interaction with
engineering and technology, psychology and ergonomics, and physical changes (to altitude,
pressures, workload, and atmosphere) (Kanas et al., 2010). Teams must be able to adapt to
the changing environment quickly and effectively.
213 Kieran Smith; Sofia Ibarra; Torin Clark; Tristan Endsley
(1641212) BROAD SUITE PHYSIOLOGICAL MODELS OF SITUATION AWARENESS
Situation awareness (SA) is critical for safety in high-stress, dangerous regimes like human
spaceflight. This study will collect physiological signals from participants operating a
simulated aircraft and completing objective SA assessments. Models will be built to estimate
SA, enabling real-time access into objective, multidimensional, and non-disruptive estimates
of human operator SA.
214 Steven Anderson; Suzanne Bell; Sheena Dev
(1641326) THE DEVELOPMENT AND APPLICATION OF COGNITIVE PERFORMANCE
ASSESSMENT FOR EXPLORATION CLASS MISSION EVAS
This project seeks to develop exploration class cognitive performance monitoring capabilities
for EVA and to provide data that can inform EVA planning for Lunar and Mars missions.
215 Lilian Felipe; Denilson Calderon
(1642126) PRE-FLIGHT INTERVENTIONS FOR MANAGING SPACE MOTION SICKNESS:
A COMPREHENSIVE REVIEW
Space motion sickness (SMS) affects over half of astronauts, causing vertigo, nausea, and
fatigue and this review explores pre-flight interventions, including drugs, exercises, and
psychological techniques, offering insights for astronaut training and mission success.
216 Sage Sherman; Allison Anderson
(1642401) A TRADE STUDY OF NON-INVASIVE BRAIN STIMULATION TECHNIQUES
FOR USE ON LONG DURATION SPACEFLIGHT MISSIONS
A trade study of five non-invasive brain stimulation methods for use as a human performance
countermeasure in long duration exploration missions. This trade study explores the
method's demonstrated utility in improving human performance and the technology's
applicability to the spaceflight environment.
217 Kihun Hong; Weiwei Zhou; Wilsaan Joiner; Stephen Robinson; Jonathon Schofield
(1645082) UNDERSTANDING TACTILE VIBRATION STROKE ON THE UPPER LIMB
FROM WEARABLE HAPTIC INTERFACE FOR ROBOTIC OPERATION TASKS
A wearable haptic interface using tactile stroke designed for the robotic arm operations in
spaceflight or on the Lunar surface provides a robust platform for examining human motor
learning, skill generalization, and transfer across robotic tasks and systems.

February 13-16, 2024 735
218 Jayanta Dhaoya; Shantanu Saha; Vijayaraghavan M; Chiranjeevi B; K Kumar

(1646078) SCALING OF SUBJECT SPECIFIC MUSCLE MODEL USING EXPERIMENTAL
STRENGTH DATA: A NOVEL APPROACH
Muscle models available in Musculoskeletal modeling software are not tailored to accurately
capture the strength capability of a specific subject. In this paper, a methodology is adopted
to tune the muscle strength index of a muscle group using experimental strength data. Such
a musculoskeletal model can assess muscle activity for several operations without the
physical presence of the subject.
219 Chiranjeevi Banala; S S Ravi Shankar; Kumar Saurav; Dhaoya Jayanta; K Kumar
(1646135) HARMONIZING HUMAN-MACHINE INTERFACE: ERGONOMIC OPTIMIZATION
IN CREW HABITAT OF A HUMAN SPACE FLIGHT
To assist in the system engineering of a human space capsule considering the human
comfort, an arm model is developed in this study. The model used experimental strength
data to optimize the placement of Human Machine Interface inside a crew habitat. Further an
experiment is conducted at the optimized posture to validate the methodology. This method
helped in reducing the developmental cost.
221 Kylie Haynie; Joi Harvey; Esther Putnam; Chad Tossell; Allison Anderson
(1646898) EVALUATING VIRTUAL REALITY TRAINING STRATEGIES FOR LONG-
DURATION EXPLORATION MISSIONS
We evaluate three different VR-Based training strategies for long-duration exploration
missions and present results based on objective performance measures and self-reported
feedback.
222 Sophia Zaccarine; David Klaus
(1647057) ATTRIBUTES OF HABITABILITY FOR SELF-SUFFICIENT DEEP-SPACE
HABITAT OPERATIONS
This work examines how the tenets of human-rated spacecraft, summarized as
accommodate (what the vehicle provides the crew), utilize (what the crew provides to the
operations), and protect (general improvements made to vehicle safety), overlap with
habitability and their contributions to self-sufficient deep-space operations.
223 Luca Bonarrigo; Esther Putman; Wyatt Rees; Benjamin Peterson; Iris Li; Sage Sherman;
Torin Clark; Dan Szafir; Allison Anderson
(1647114) QUANTIFYING NEURAL ACTIVATION DURING SPACEFLIGHT-RELEVANT
VIRTUAL REALITY TRAINING
By quantifying neural activation in areas of interest during task completion in an operational
environment, we aim to inform a gap in knowledge necessary to investigate whether a
relevant VR training environment could serve as an effective countermeasure for spaceflight
neural decrements.
224 Sarah Leary; Allison Anderson; Torin Clark; Victoria Hurd; Christian Lee; Jaekeun Sung
(1647143) METRICS AND MODELS TO INFER TRUST IN HUMAN-AUTONOMY TEAMING
Our goal is to model reported trust using physiological responses and embedded measures,
which will inform the development of metrics and models that can infer and predict trust.
225 Allison Anderson; Esther Putman; Sage Sherman; Luca Bonarrigo; Ellery Galvin; Alessandro
Verniani; Sandra Tredinnick; Karen Mae Baldonado; Eric Vance; Stephen Robinson; Daniel
Szafir; Torin Clark
(1647249) TRINITY: MULTI-ENVIRONMENT VIRTUAL TRAINER FOR LONG DURATION
EXPLORATION MISSIONS
This research investigates virtual reality as a medium for training astronauts on long duration
exploration missions by developing a multi-environment virtual training simulator called
Trinity.

February 13-16, 2024 745
226 Tina Panontin; John Karasinski; Lauren Landon; Katie McTigue; Megan Parisi; Alonso Vera;
Shu-Chieh Wu
(1647299) BEYOND LOW EARTH ORBIT: UNDERSTANDING IMPACTS OF
COMMUNICATION DELAYS
Results from a systematic review of 20 years of publications and a pilot study simulating
lunar type (10-sec round trip) comm delay effects on complex tasks will be presented to help
characterize the risk posed by communication delay for upcoming Artemis missions and
point toward potential mitigations.
227 Tina Panontin; Kritina Holden; Mercedes Jorge; John Karasinski; Katie McTigue; Amanda
Smith; Alonso Vera; Megan Parisi; Shu-Chieh Wu
(1647366) INFORMATION REPRESENTATIONS FOR ONBOARD, CREW-LED PROBLEM
SOLVING
This work seeks to understand the content and representation of information needed to
support time-constrained problem solving and decision making by crews under conditions of
delayed communications with the ground team.
228 Patrick Pischulti; David Klaus
(1648285) SIMULATION OF AN AUTONOMOUS ANOMALY RESPONSE ARCHITECTURE
FOR HUMAN DEEP-SPACE EXPLORATION MISSIONS
Utilizing model-based systems engineering (MBSE) to assess the performance of an Earth-
independent anomaly response architecture and to identify mission enabling human systems
integration architecture (HSIA) elements.
229 Lucas Brane; Mary Cooper; Madison Diamond
(1648451) ONE SMALL STEP: A LOOK AT THE IMPACT OF OPENING CREW
POSITIONS UP TO BELOW THE KNEE PROSTHETIC USERS ON ANALOG ASTRONAUT
MISSIONS
This study, which explores the inclusion of a crew member with a prosthetic in an analog
setting, is aimed at identifying any impacts in performance, time considerations, equipment
adaptations, and mission planning with the goal of providing spaceflight entities with a
foundation to design broadly inclusive infrastructure and operations.
230 Maneesh Verma; Lucie Ráčková
(1648576) SMALL SAMPLE, BIG IMPACT: STRATEGIES FOR OPTIMIZING HUMAN-
ROBOT INTERACTION STUDIES IN SPACE ANALOG MISSIONS
This work presents an optimal method combination and selection process to maximize
information gain and strengthen the validity of research outputs for conducting research on
human-robot interaction in a space analog mission, specifically illustrated using an example
of multiple controller comparisons for local remote operation, out of line of sight.
231 Erin Richardson; Savannah Buchner; Allison Anderson
(1648608) TRANSFERABILITY OF PHYSIOLOGICAL MODELS OF HUMAN TRUST,
WORKLOAD, AND SITUATION AWARENESS
We develop models of TWSA from electrocardiogram, respiration, and eye tracking data
recorded as operators perform different spaceflight-relevant tasks and then assess the ability
of the models to transfer to both new participants and to new tasks.
232 Jenna Korentsides; Joseph Keebler
(1648626) THE USE OF AI AND AUTOMATION FOR CREW ADVANTAGES IN DEEP
SPACE
Space exploration challenges human limits, emphasizing the need for technological
intervention. AI and automation play crucial roles in addressing these challenges. From a
human factors perspective, AI and automated systems can aid in a multitude of tasks that will
help to lessen cognitive and physical workloads among the astronaut crew.
233 Stephen Robinson; Brian Huo
(1649171) HAPTIC FEEDBACK AS A SENSORY AID FOR MANUAL CONTROL OF SPACE
ROBOT ARMS
Recent research progress on haptic feedback to aid manual control of space robot arms.

February 13-16, 2024 755
234 Steve McGuire; Matt Rochford; Christine Fanchiang; Michael Zero

(1649491) MACHINE LEARNING-BASED BIOSIGNAL ANALYSIS WITH APPLICATION TO
BANDWIDTH-CONSTRAINED ENVIRONMENTS
This work proposes a new method for analyzing raw biosignals based on a transformer
neural network architecture that learns salient signal features in a low-dimensional space,
enabling subsequent transmission and decoding into cognitive state estimates, raw signal
regeneration, and traditional vital measurements.
235 Poonampreet Kaur Josan; Prachi Dutta; Bonnie Dunbar; Raymond Wong; Daniel Selva; Ana
Diaz-Artiles
(1649601) RESULTS FROM HERA ANALOG TESTING OF A VIRTUAL ASSISTANT FOR
ANOMALY RESOLUTION
A virtual assistant (VA) was developed and deployed in NASA HERA Analog to understand
how VAs can be used during Long duration exploration missions (LDEMs) and to measure
their effect on crew member's performance metrics. The abstract provides an overview of the
experiment and discuss the results obtained.
236 Samuel Kurtin; Allison Anderson
(1649635) LONGITUDINAL INVESTIGATION OF HUMAN TRUST IN AUTONOMY FOR
OPERATIONAL ENVIRONMENTS
Longitudinal trust dynamics of human-autonomy teaming are investigated through rigorous
qualitative interviews with individuals who interact with robotic and autonomous systems.
237 Prachi Dutta; Ana-Diaz Artiles; Bonnie Dunbar; Raymond Wong; Daniel Selva; Poonampreet
Josan
(1649673) EFFECT OF AGENT ACCURACY, CONFIDENCE, AND TRANSPARENCY ON
THE EVOLUTION OF TRUST IN AI-ASSISTED ANOMALY DIAGNOSIS
The primary objective of this work is to provide evidence on the effects of agent accuracy,
confidence (uncertainty in its recommendation), and transparency (amount of explanations)
on an operator’s trust adaptation over time in an anomaly diagnosis task. The abstract
provides an overview of the experiment and discuss the results obtained.
238 Shawna Pandya; Mike Wesolowski
(1650059) IMMERSIVE TECHNOLOGIES FOR TRAINING AND SIMULATION IN
SPACELIKE ENVIRONMENTS
In this study, we describe our experiences deploying a VR-headset in a pressurized extra-
vehicular activity (EVA) spacesuit in a gravity off-set system.
239 Alexander Chin; Szu-Jui Chen; John Hansen
(1650118) A COMPARATIVE STUDY FOR THE HUMAN EXPLORATION RESEARCH
ANALOG BASED ON SPEECH RECOGNITION AND SPEAKER DIARIZATION
NASA's HERA program studies human health and performance in long-duration space
missions, simulating astronaut conditions. The study uses speech recognition/diarization to
compare transcribed LENA audio word error rates with previous setups. Improving
transcription accuracy enhances HERA researchers' capacity for a comprehensive analysis
of team communications in a more timely process.
240 Anurag Verma
(1653526) DESIGN OF HUMAN MACHINE INTERFACE FOR CREWED SPACEFLIGHT OF
GAGANYAAN
The paper presents approach towards achieving a human machine interface with ergonomic
design of console wihting mission constraints.

February 13-16, 2024 765
Poster Session B: Space Biology

284 Kira Rienecker; Tristen Head; Amelia Espinosa; Macarena Parra; Michael Padgen; Milton
Santos Cordeiron; Morgan Anderson; Jessica Koehne; Antonio Ricco; Christian Espinozan;
Victor Yeh; Yasaman Shirazi-Fard
(1642161) MICROFLUIDIC QUANTIFICATION OF CIRCULATING MICRORNA: TOWARDS
LONGITUDINAL MONITORING INDIVIDUALIZED RESPONSES TO SPACEFLIGHT
STRESSORS
We are developing an automated microfluidics system to quantify microRNA as candidate
biomarkers of the individual response to space radiation and other spaceflight stressors.
285 Ananya Bansal; Kira Rienecker; Connie Pasternak; Sonali Verma; Egle Cekanaviciute;
Sylvain Costes
(1642225) COMPARATIVE RADIOSENSITIVITY OF HUMAN AND MOUSE IMMUNE CELL
TYPES EX VIVO
We performed a quantification and comparison of DNA damage responses in human and
mouse immune cells irradiated ex vivo to compare radiosensitivity between cell types,
species and irradiation conditions.
286 Su-Geun Yang; Kyung-Ju Shin; Hye Jin Yoo; Steve K. Cho; Kyu-Sung Kim
(1644664) MITOCHONDRA TARGETING ANTIOXIDANT FOR PROTECTION OF
MICROGRAVITY-INDUCED OXIDATIVE STRESS
In this study, we validated the effectiveness of mitochondria-targeting antioxidant in
mitigating µG-induced oxidative stress and mitochondrial dysfunction in vitro.
287 Natasha Haveman; Cory Spern; Anirudha Dixit; Mary Hummerick; Christina Khodadad
(1645266) ENHANCING THE AUTOMATION OF NUCLEIC ACID EXTRACTION FOR
FOOD SAFETY ANALYSIS FOR FUTURE ON-ORBIT ASSESSMENT.
Comparing the traditional method of extracting DNA and identifying microbes isolated from
food crops grown in EDEN ISS with newly developed automation-primed plant microbial
nucleic acid extraction approaches.
288 Peng Huang; Cuiping Zhang; Abba Zubair
(1645545) IMPACT OF MICROGRAVITY ON BONE HOMEOSTASIS RELATED GENES
Our study suggests gene expression following MSC culture under microgravity condition at
ISS was significantly changed regarding of bone homeostasis.
289 Jung-uk Shim; Byeongkon Kim; Jinyang Chung; David Jeon; Tae-Don Kim; Hargsoon Yoon
(1646122) CONTROLLING GRAVITY TO FACILITATE GENE ELECTROPORATION
We present that controlling gravity is a new and potentially powerful tool to transfect genetic
materials into cells with a high cell viability and transfection efficiency in comparison to
traditional electroporation.
290 Sarah Kingsley; Ana Duran-Viseras; Kostas Konstantinidis; Thomas Orlando
(1646230) DETECTION AND IDENTIFICATION OF MICROORGANISMS BY VOC
ANALYSIS
This project aims to explore the use of volatile organic compound (VOC) profiling as a
microbial monitoring technique by developing a method for the detection, identification, and
analysis of microorganisms based on the VOCs emitted through microbial metabolism.
291 Michael Cornforth; Stephen Kunkel; Bradford Loucas
(1647246) CHROMOSOME DAMAGE AND TEMPORAL ASPECTS OF GCR SIMULATION:
VALIDATION AND BEAM TIME OPTIMIZATION
This work proposes to relate chromosome damage, as measured by mFISH to temporal
constraints of GCR simulation for the purposes of validation and beam time optimization.

February 13-16, 2024 775
292 Marissa Burke; Nadia Houerbi; JangKeun Kim; Eliah Overbey; Sonia Villapol; Christopher
Mason
(1647420) EXPLORING SPACEFLIGHT EFFECTS ON THE MICROBIOME-GUT-BRAIN-
AXIS AND IMMUNE FUNCTION.
Analysis of spaceflight affects on direct CNS and peripheral communication of the gut-brain-
axis using multiple models, including Inspiuratiopn 4 astronaut secretome samples, ground-
based analogs on mice, and NASA open-source studies.
293 Sigrid Reinsch; Joseph Varelas; Mo Kaze; Samrawit Gebre
(1647766) THE NASA BIOLOGICAL INSTITUTIONAL SCIENTIFIC COLLECTION (NBISC):
TISSUE AND MICROBE BIOSPECIMENS TO ADVANCE SPACE RESEARCH
NASA’s Biological Institutional Scientific Collection (NBISC) houses specimens from
spaceflight and ground studies including > 50,000 biospecimens from HRP’s Biospecimen
and Tissue Sharing Collection (BTSC) Program: samples from HRP’s Space Radiation
Element-funded studies using rodents exposed to radiation protocols at the NASA Space
Radiation Laboratory (NSRL) and other analog facilities.
294 Daniel Brassard; Karine Turcotte; Keith Morton; Liviu Clime; Matthias Geissler; Sam Ng; Tim
Fielding; Denis Charlebois; Teodor Veres
(1647903) MICROPREP: ADVANCED CENTRIFUGAL MICROFLUIDIC PLATFORM FOR
THE AUTOMATION OF COMPLEX SAMPLE PREPARATION PROCEDURES IN THE ISS
We report on MicroPREP, an advanced pneumatic centrifugal microfluidic platform planned
for ISS deployment in early 2025 that has the capacity to automate a wide range of complex
sample preparation procedures with minimal crew time and therefore greatly facilitate
deployment of various ISS biology and life science experiments.
295 Masafumi Muratani; Nailil Hsna; Lindsay Rutter
(1648015) COMMON AND PERSONALIZED SPACE ENVIRONMENTAL RESPONSES
DETECTED BY JAXA CELL-FREE EPIGENOME STUDY WITH SIX ASTRONAUTS
JAXA Cell-Free Epigenome Study shows that an N&#3f6 astronauts plasma cell-free DNA
and RNA analysis provides useful results to detect both common and individualized space
environmental responses, which is critical information for effectively designing future
astronaut multi-omics studies with limited numbers of participants.
296 Daniela Bezdan; Olaf Riess; Maria Birlem; Christian Bruderrek; Daniel Kaschubek; Mark
Kugel; Stephan Ossowski; Kevin Achberger; Stefan Liebau
(1648041) FULLY AUTOMATED, AFFORDABLE, AND SCALABLE LIFE SCIENCE
EXPERIMENTS IN LOWER ORBIT: INNOVATIONS, CHALLENGES, AND FUTURE
PROSPECTS.
With astronaut time being costly and the rise in uncrewed missions, there's a surge in
demand for cost-effective, automated systems. We unveil Yuri's automated minilabs,
featuring a compact microscope and gravity-simulating incubator. Insights into past and
upcoming research in space medicine, synthetic biology, and terrestrial healthcare
applications are also shared.
297 Sungryeal Kim; Kyu-Sung Kim; Jeong-Seok Choi
(1648045) THE EFFECT OF SIMULATED MICROGRAVITY ON CELL CYCLE AND
PROLIFERATION OF SALIVARY GLAND DERIVED STEM CELL
After simulated microgravity treatment, the proliferation of salivary gland derived stem cell
increased.
298 Andie Padilla; Binata Joddar; Candice Hovell; Jeremy Mares; Veerle Reumers
(1648294) ELECTROPHYSIOLOGICAL RECORDING OF HUMAN NEURONAL
NETWORKS DURING SUBORBITAL SPACEFLIGHT
Electrophysiological recording from a human neuronal tissue on a chip to assess the effects
of a suborbital spaceflight on neuronal networks.

February 13-16, 2024 785
299 Bailey McFarland; Cheng Chen; Robert Hinshaw; Sylvain Costes; Egle Cekanaviciute; Yu
Huang
(1648686) HUMAN BRAIN ORGANOID RESPONSES TO SIMULATED MICROGRAVITY
AND IONIZING RADIATION
We present the analysis of brain organoid responses to simulated microgravity and simulated
space radiation, including the effects of radiation dose rate.
300 Catriona Jamieson; Jane Isquith; Jessica Pham
(1649135) STEM CELL PATHWAYS, AGING, AND CANCER EVOLUTION (SPACE)
To investigate stem cell pathways, aging, and cancer evolution (SPACE), the NASA
Integrated Space Stem Cell Orbital Research (ISSCOR) program and Sanford Stem Cell
Institute (SSCI) engineered human hematopoietic stem cells and tumor organoid
nanobioreactors and developed multi-omic and functional analytical methods to determine
stem cell fitness before, during, and after space flight.
303 Minjin Kim; Su A Kim; Jinwook Shin
(1649562) EFFECTS OF SIMULATED MICROGRAVITY ON MAST CELL FUNCTION AND
HOMEOSTASIS
Murine bone marrow-derived mast cells (BMMCs) were cultured with IL-3 in a rotary cell
culture system (RCCS) that generates a simulated microgravity (SMG) environment. BMMCs
exposed to SMG showed enhanced apoptosis, and reduced proliferation compared to Earth's
gravity (1G) controls. In addition, SMG impaired mast cell degranulation and cytokine
secretion.
304 Christopher Porada; James Rice; Jonathan Diaz; Ritu Ramamurthy; Alan Jacobson; Sean
Murphy; Thomas Shupe; Anthony Atala; Graça Almeida-Porada
(1649676) LONG-LIVED SINGLE- AND MULTI-ORGAN TISSUE EQUIVALENT (OTE)
PLATFORMS TO MODEL THE RESPONSE OF HUMAN TISSUES TO VARIOUS
STRESSORS
We have created and are using long-lived single- and multi-organ tissue equivalent (OTE)
platforms to model the response of human tissues to various spaceflight stressors, including
VOCs, regolith, infectious agents, and ionizing radiation.
305 Siddhita Mhatre; Janani Iyer; Steffy Tabares Ruiz; Stephanie Puukila; John Hayes; Amber
Paul; Candice T. Tahimic; Yasaman Shirazi-Fard; Marianne Sowa; Joshua Alwood; April
Ronca
(1649844) NEUROBIOLOGICAL OUTCOMES OF MICE EXPOSED TO COMBINED
SPACEFLIGHT STRESSORS
Neurobiological outcomes of mice exposed to combined spaceflight stressors
306 Anand Narayanan; Caleb Towne; Michael Delp
(1650527) SIMULATED DEEP SPACE RADIATION EXPOSURE CAUSES LONG-TERM
EFFECTS WITH CERVICAL LYMPHATIC BIOLOGY
Astronauts on missions to the Moon will face health risks from radiation exposure that is
more deleterious than that found during LEO missions. Furthermore, the lymphatics are an
understudied area of the cardiovascular system in the context of spaceflight adaptations.
Thus, the purpose of this study is to investigate the effects of deep space radiation on
cephalic lymphatic structure and function.
307 Jhan Saavedra Torres; Maira Alejandra Guayambuco Medina
(1652544) CELL AND MOLECULAR BIOMECHANICS: IMPACT OF SIMULATED
MICROGRAVITY ON CYTOSKELETON.
In this review, we provide a historical overview of research evaluating the effects of
spaceflight on Impact of Simulated Microgravity on Cytoskeleton and outline mechanisms
underpinning spaceflight-related Cytoskeleton alterations.

February 13-16, 2024 795
308 Jhan Saavedra Torres; Maira Alejandra Guayambuco Medina; Jhan Saavedra Torres
(1655802) THE IMPACT OF MICROGRAVITY AND HYPERGRAVITY: MODELING OF
CONCURRING EVENTS RESULTING IN THE PRO-ANGIOGENIC EFFECT OF
HYPERGRAVITY.
The impact of microgravity and hypergravity: Modeling of concurring events resulting in the
pro-angiogenic effect of hypergravity.

February 13-16, 2024 805
Poster Session B: Spaceflight Associated Neuro-ocular Syndrome (SANS)

141 Michael Bodo; Sandor Szabo; Mihaly Bagany; Istvan Pinter
(1635712) NONINVASIVE MEASUREMENT OF INTRACRANIAL PRESSURE AND
CEREBRAL BLOOD FLOW AUTOREGULATION: A RHEOENCEPHALOGRAPHY STUDY
Noninvasive REG results documented the change of CBF AR and increased ICP during HDT
position. Developed software calculates these changes. REG electrodes are from conductive
fabric and are reusable; measurement is suggested before or after OCT and fundoscopy to
establish cerebrovascular components of SANS.
142 Laura de Boni; Alexandru Odainic; Maria Bohmeier; Petra Frings-Meuthen; Edwin Mulder;
Jan-Niklas Hönemann; Karsten Heusser; Susanne Schmidt; Jens Tank; Jens Jordan
(1636199) NO SEROLOGICAL EVIDENCE FOR NEURONAL DAMAGE OR REACTIVE
GLIOSIS DURING 30 DAYS HEAD-DOWN TILT BEDREST
We did not observe clinically relevant neurological deficits or major changes in circulating
neuronal or glial biomarkers (Tau, GFAP, Amyloid-beta 40 (Aβ40), Amyloid-beta 42 (Aβ42),
NfL) in blood following 30 days HDT with or without countermeasures mitigating cephalad
fluid shifts.
143 Eric Bershad; Anders Eklund; Mohammad Hirzallah; Jan Malm; Michael Williams
(1641991) ENHANCING EXPLORATION PLATFORMS AND ANALOG DEFINITION -
INTRACRANIAL PRESSURE (EXPAND-ICP)
The purpose of this study is to measure the ICP using the lumbar puncture technique before
and after spaceflight in astronauts on a commercial spaceflight mission to assess for
changes in ICP related to spaceflight, and correlate to any SANS findings.
144 Larry Kramer; Khader Hasan; John Lincoln; Xu Zhang; Raj Iyer; Stephanie Lee; Grace Wang
(1642969) DURAL VENOUS SINUS VOLUMETRY DERIVED FROM CONTRAST-
ENHANCED MAGNETIC RESONANCE IMAGING
This study aims to establish a contrast-based dural venous sinus magnetic resonance
imaging volumetry protocol, document adult segmental dural venous sinus volumes, and
determine inter-observer reliability to understand better the pathophysiology and risk factors
associated with spaceflight-associated neuro-ocular syndrome.
145 Peter zu Eulenburg; Ge Tang; Seyed-Ahmad Ahmadi; Steven Jillings; Ben Jeurissen; Elena
Tomilovskaya; Angelique Van Ombergen; Floris Wuyts
(1643052) PHYSIOLOGICAL LINK BETWEEN BRAIN AND EYE ACCOUNTS FOR THE
CONCURRENT REMODELING OF BOTH STRUCTURES IN LONG-DURATION
SPACEFLIGHT
Neuroimaging-based ocular morphometrics robustly showed that the eye, the retroorbital
space, and the optic nerve responds in lockstep with the central chambers of the
cerebrospinal fluid system to longer stays in microgravity aboard the International Space
Station.
146 Katrina Carter; Aaron Betts; Valerie Ryder; Fabian Möller; Andrew Lovering; Erin Flynn-
Evans; Brandon Macias; Steven Laurie
(1645072) MEASUREMENTS OF TRANSCUTANEOUS PCO2 WITH TCM5 MONITOR
DURING SPACEFLIGHT
Mildly elevated CO2 levels on the International Space Station (ISS) are a concern to the
health of crew, yet the impact these ambient levels have on crew is largely anecdotal. The
purpose of this study is to characterize CO2 in crewmembers on the ISS throughout the day
and during sleep and determine if variability in CO2 levels on the ISS are associated with
negative outcomes.

February 13-16, 2024 815
147 Katrina Carter; Mathias Basner; Eric Bershad; Rachael Seidler; Alexander Stahn; Steven
Laurie; Brandon Macias
(1645094) INVESTIGATING LONG-TERM STRUCTURAL AND FUNCTIONAL CHANGES
TO THE EYE AND BRAIN AFTER SPACEFLIGHT
The long-term consequences of spaceflight on the brain and eye—either related to or
independent of SANS—are largely unknown. This project will evaluate the persistence of
spaceflight-induced ocular, brain, and behavioral changes, as well as quantify their
association with long-term health and performance.
148 Hayley Brawley; Travis Hein; Scott Smith; Patrick Stover; Sara Zwart
(1645352) IN SEARCH OF A RODENT MODEL OF SPACEFLIGHT ASSOCIATED NEURO-
OCULAR SYNDROME USING ONE-CARBON GENETICS
There is no rodent model of SANS; therefore, we set out to determine if a mouse model of
human genetic variation for one-carbon metabolism results in pathologies similar to those
observed in SANS.
149 Lonnie Petersen; Justin Lee; Benjamin Levine; Casper Petersen
(1646439) LOWER BODY NEGATIVE PRESSURE SUIT AS AN INTEGRATIVE
COUNTERMEASURE DURING SPACEFLIGHT
Wearable and mobile LBNP is a promising and feasible integrative countermeasure to
maintain human health and perfomance during exploration class missions
150 Travis Hein; Walter Cromer; Robert Rosa; Binu Tharakan; David Zawieja
(1646443) MARKERS OF VASCULAR RISKS ASSOCIATED WITH SPACEFLIGHT
ASSOCIATED NEURO-OCULAR SYNDROME
This study will determine what biomarkers of spaceflight-associated ocular vascular
dysfunction in the cerebrospinal fluid of crewmembers, which will be collected before and
after missions on the ISS, might be useful to monitor and address SANS.
151 Charles Belanger Nzakimuena; Marissé Masis Solano; Mark Lesk; Santiago Costantino
(1646638) TRANSFER LEARNING FOR CHOROID SEGMENTATION
Transfer learning is leveraged to enable a pre-trained deep learning model to generalize and
accurately segment thicker than average astronaut choroids on macular optical coherence
tomography images.
152 Nicole Bodi; Shahna Hameed; Tasneem Sharma
(1646868) DETERMINING THERAPEUTIC POTENTIAL OF COENZYME Q10 IN
MICROGRAVITY SIMULATED HUMAN RETINAL MODELS
CoQ10 depicted therapeutic potential in the human retina by increasing antioxidant driven
genes which reduced deleterious effects of oxidative stress.
153 Tasneem Sharma; Nicole Bodi; Shahna Hameed; Seta Stanbouly; Marcelo Vazquez; Xiao
Mao
(1646897) UNDERSTANDING HUMAN OCULAR IMPACTS FOR SPACE RADIATION
Our study validates that there is increased oxidative stress in human retinal tissue after
exposure to simulated spaceflight radiation conditions.
154 Brandon Macias
(1647330) CIPHER: INVESTIGATING STRUCTURE AND FUNCTION OF THE EYE
(ISAFE)
The purpose of the ISAFE study is to determine 1) the incidence and magnitude of ocular
structural and functional changes increase with mission durations of up to one year, 2)
postflight recovery of those alterations is protracted following longer duration missions, and
3) the degree of vascular adaptation correlates with the magnitude of the ocular and
neurological findings.

February 13-16, 2024 825
155 Brandon Macias

(1647381) MITIGATING HEADWARD FLUID SHIFTS WITH VENO-CONSTRICTIVE THIGH
CUFFS DURING SPACEFLIGHT (FLIGHT THIGH CUFF STUDY)
The purpose of this study is to evaluate the efficacy of using the NASA VTC to reverse the
headward fluid shift during spaceflight and inform use of VTC as a potential SANS
countermeasure.
156 Mohammad Hirzallah; Piergiorgio Lochner; Muhammad Hafeez; Andrew Lee; Christos
Krogias; Deepa Dongarwar; Nicholas Hartman; Michael Ertl; Chiara Robba; Felix
Schlachetzki
(1648138) OPITC NERVE SHEATH DIAMETER ULTRASONOGRAPHY QUALITY
CRITERIA CHECKLIST (ONSD POCUS QCC) : AN INTERNATIONAL CONSENSUS
PROJECT TO STANDARDIZE ONSD MEASUREMENT AND SPACE FLIGHT
IMPLICATIONS
Optic nerve sheath diameter have limited clinical utility due to variation in imaging methods,
structures included in the measurements, and cut offs used to define elevated intracranial
pressure. A consensus panel with 60 international experts (including NASA ONSD experts)
have put together standardized ONSD imaging and measurement criteria to address this
issue.
157 Marisse Masis Solano; Rémy Dumas; Mark Lesk; Costantino Santiago
(1648895) OCULAR BIOMECHANICAL CHANGES IN ASTRONAUTS FOLLOWING LONG
TERM SPACE MISSIONS
This study examined ocular biomechanical changes in astronauts after long-term space
missions. Results showed a significant decrease in ocular pulse amplitude (25.2%) and
intraocular pressure (10%) along with reduced ocular rigidity (36.2%). These findings
highlight microgravity's impact on eye health, offering insights into space flight-related neuro-
ocular syndrome.
158 Polona Jaki Mekjavic; Tinkara Mlinar; Winfried Amoaku; Grégory Geronton; Pierre Denise;
Igor Mekjavic
(1649267) SPACEFLIGHT ASSOCIATED FACTORS AFFECTING INTRAOCULAR
PRESSURE
The effects of spaceflight associated factors on introcular pressure (IOP), including age,
gender, posture (upright, supine and prone 6° head down tilt supine, HDT), hypecapnia,
static exercise and hypoxia (in progress) demonstrate that static exercise performed by older
participants in the prone 6°HDT position significantly increases IOP, with hypercapnia and
age exacerbating the response.
159 Susan Voss; Stijn Thoolen; Stefan Moestl; Timo Frett; Gary Strangman
(1649551) BRAIN-SANS: WHAT DO DPOAES TELL US ABOUT INTRACRANIAL
PRESSURE CHANGES DURING EXPERIMENTS DESIGNED TO UNDERSTAND SANS?
At the DLR’s :envihab facility, we measured changes in distortion product otoacoustic
emissions (DPOAEs) to monitor intracranial pressure changes associated with 30-day, 6
degrees head-down tilt bedrest—with and without countermeasures—as part of the SANS-
CM campaigns.
160 Mimi Lan; Bofan Chen; Jay Buckey
(1649575) A CASE DEFINITION IS NEEDED FOR THE SPACEFLIGHT ASSOCIATED
NEURO-OCULAR SYNDROME
This study explored the linear relationship between change in total retinal thickness and
presentation of SANS signs and symptoms. Change in choroid thickness and retinal nerve
fiber layer was also examined.
201 Mimi Lan; Bofan Chen; Jay Buckey
(1649587) EYE LENGTH IS A POTENTIAL PREDICTOR OF SPACEFLIGHT-RELATED
VISUAL CHANGES
This study used anthropometric data from the LSAH and machine learning to identify
important predictors of spaceflight visual change. Preflight eye axial length was identified as
an important predictor.

February 13-16, 2024 835
202 Saikat Mukherjee

(1649699) NUMERICAL MODELING OF CEREBROSPINAL FLUID-INDUCED OPTIC DISC
EDEMA IN ASTRONAUTS
I am proposing to model the cerebrospinal fluid (CSF) flow disruption in the perivascular
spaces surrounding the vasculature in the optic nerve sheath (glymphatic system - a recent
breakthrough in neuroscience) under exposure to microgravity and investigating the role of
the altered glymphatic CSF flow in the optic disc edema risk in astronauts.
203 Saravanakumar Muthusamy; Felicia Hunter; O'lisa Waithe; Steven Laurie; Brandon R
Macias; Pooneh Bagher; Travis W Hein; David Zawieja; Binu Tharakan
(1649902) EVIDENCE FOR BLOOD-RETINAL BARRIER HYPERPERMEABILITY IN MICE
EXPOSED TO MICROGRAVITY
This study suggests preliminary evidence for retinal microvascular hyperpermeability
following exposure to microgravity/spaceflight
204 Fabian Möller; Aaron Betts; K Carter; Michael McGeehan; Valerie Ryder; Brandon Macias;
Steven Laurie; Andy Lovering
(1650018) TRANSCUTANEOUS MONITORING OF PARTIAL PRESSURE OF OXYGEN IS
INADEQUATE
Validity of transcutaneous oxygen partial pressure measurements under hypoxia in a ground-
based study
205 Aaron Betts; Katrina Carter; Steve Laurie; Andrew Lovering; Fabian Möller; Brandon Macias
(1650297) VALIDATION OF GROUND-BASED MEASUREMENTS OF
TRANSCUTANEOUS PCO2 WITH TCM5 MONITOR USING DIRECT ARTERIAL
MEASURES
This ground-based study aims to validate a transcutaneous CO2 (PTCCO2) monitor
(Radiometer TCM5) against direct gold standard measures of arterial blood CO2 (PaCO2).
206 Stuart Sater; Katherine Warthen; Omolola Bangudu; Larry Kramer; Khader Hasan; Michael
Williams; Brandon Macias; Steven Laurie; Bryn Martin
(1650310) SEMI-AUTOMATED MRI-BASED SEGMENTATION OF THE SPINAL CENTRAL
NERVOUS SYTEM FOR QUANTIFICATION OF SPINAL CHANGES IN HEAD-DOWN-TILT
SUBJECTS
A novel method by which the spinal cord and spinal cerebrospinal fluid (CSF) can be semi-
automatically segmented to quantify center of gravity, and volumetric changes during and
after HDT-BR was developed and has been implemented in a preliminary dataset.
207 Aaron Betts; Anni Ewald; Andrew Lovering; Brandon Macias; Karina Marshall-Goebel; Edwin
Mulder; Steve Laurie; Alexandra Noppe; Laura Pardon; Carla Petitti; Millenia Young
(1650339) IMPACT OF MECHANICAL AND GRAVITATIONAL COUNTERMEASURES ON
SLEEP AND OCULAR CHANGES DURING STRICT HEAD-DOWN TILT BEDREST
Impact of mechanical and gravitational countermeasures on sleep (i.e., total sleep time,
wake after sleep onset, stages of non-rapid eye movement sleep) and ocular changes from
SANS bedrest countermeasure campaigns 1-4.
208 Stuart Sater; Katherine Warthen; Larry Kramer; Khader Hasan; Michael Willimas; Brandon
Macias; Steven Laurie; Bryn Martin
(1650352) POSTERIOR OPTIC GLOBE VOLUME DISPLACEMENT DURING 30-DAY
HEAD-DOWN TILT BEDREST AND THE EFFECT OF POTENTIAL COUNTERMEASURES
Quantification of globe flattening in OPTICS study HDT-BR subjects.
209 Cesar Escamilla-Ocanas; Noelia Morales-Cardona; Alireza Akhbardeh; Mohammad Hirzallah
(1650396) DEVELOPMENT OF AN AUTOMATED ALGORITHM FOR OPTIC NERVE
SHEATH DIAMETER MEASUREMENT USING TRANSORBITAL ULTRASOUND
Ultrasound ONSD is portable and non-invasive tool for ICP measurement during spaceflight,
however its practicality has been limited by variability in imaging and measurement
techniques. 52 de-identified transorbital sonographic scans will be used for development of a
an automated machine-learning algorithm. Automated ONSD measurements might be a
valuable tool to monitor for SANS during missions.

February 13-16, 2024 845
210 Michael Williams; Eric Bershad; Benjamin Levine; Jonathan Clark; David Furman; Xiao Hu;
Jan Malm; Anders Eklund; Tomas Bäcklund; Brandon Macias; Steven Laurie
(1650438) ZERO G AND ICP: INVASIVE AND NONINVASIVE ICP MONITORING AND
SANS BIOMARKER IDENTIFICATION
We present the progress on the Direct ICP protocol for pre-/post-flight lumbar puncture for
ICP measurement and biomarker collection and analysis.

February 13-16, 2024 855
Poster Session B: SR Central Nervous System (CNS)

301 Jason Ciola; Laura Wieg; Fidelia Gaba; Brianna Colletti; Robert Hinshaw; David Holtzman;
Takashi Saito; Hiroki Sasaguri; Takaomi Saido; Cynthia Lemere
(1637801) TRAVEL-, SEX- AND APOE-SPECIFIC LATE CNS EFFECTS OF GCRSIM AND
GAMMA RADIATION IN AGED APP KNOCK-IN MICE ON A HUMANIZED APOE3 OR
APOE4 BACKGROUND
New data furthering investigation of the travel-, sex-, and ApoE-specific late CNS effects of
GCRsim and Gamma irradiation in aged APP Knock-in mice on a humanized ApoE3 or
ApoE4 background.
302 Nina Li; Simran Phuyal; Richard Britten
(1642323) THE EFFECTS OF SPACE RADIATION EXPOSURE ON RISK BEHAVIOR IN
RATS
The objective of this study is to investigate the impact of low doses of simulated space
radiation (GCRSim) on risk behavior of female rats.
336 Zachary Luyo; Riley Heerbrandt; Alea Boden; Namrata Singh; Hwang Jackie; Richard
Britten; Laurie Wellman; Larry Sanford
(1643557) BEHAVIORAL SEX DIFFERENCES IN RATS EXPOSED TO SPACE
RADIATION AND SOCIAL ISOLATION
In this project, we utilized balance beam (BB) and bilateral tactile adhesive removal (BTAR)
tasks to assess potential sex differences in the effects of SR and SI on sensorimotor
performance.
337 Mark Osabutey; Kerry O'Banion; John Olschowka
(1649991) POPULATION-LEVEL ANALYSIS OF MICROGLIAL INFLAMMATORY STATES
AFTER LOW-DOSE SPACE RADIATION EXPOSURE
Our study employs single-cell RNA sequencing and cell hashing to investigate microglial
responses to space radiation, attempting to uncover potential sex-dependent effects and
previously uncharacterized subpopulations.
338 Shanice Manning; Nousha Afshari; Jeffery Chancellor; Maria Kratz; Wayne Newhauser
(1650538) HIGHLY PRECISE COMPUTATIONAL PHANTOMS OF MOUSE AND
MARMOSET ANATOMY FOR EVALUATING DOSE TOPOLOGY
This project investigates utilizing high-resolution computational models to be used to
characterize network-level functional changes resulting from charged particle radiation-
induced perturbations to cell structure and membrane properties in the mouse and marmoset
brains .
339 Ashley Blackwell; Richard Britten; Susan Gleed
(1650767) CO-MORBIDITY OF DEFICITS BETWEEN ATTENTIONAL SET SHIFTING AND
STRING-PULLING BEHAVIOR IN MALE IRRADIATED (4HE AND 5-ION GCRSIM) RATS
The co-morbidity of deficits on attentional set shifting and string-pulling tasks were evaluated
in male irradiated (4He or GCRsim) rats. Subgroups of rats with high (100% Sham, 41%
4He, 42% GCRsim), low (12% 4He), and a mix of high/low performance were identified on
measures derived from these tasks. 4He-exposed rats may be at a high risk of disruptions to
attention and fine motor control.

February 13-16, 2024 865
340 Premkumar Saganti; Huichen Wang

(1652426) HIGH THROUGHPUT, REAL-TIME, LABEL-FREE HUMAN CELL ASSAYS FOR
SPACE RADIATION STUDIES AND COUNTERMEASURES
Our earlier studies and preliminary data on mouse neuronal cells and immortalized human
hippocampal neurons indicate that the persistent DNA breaks are repaired by an alternative
non-homologous end-joining (Alt-NHEJ) pathway dependent on Poly(ADP-Ribose)
polymerase 1(PARP-1) and ATM (Ataxia Telangiectasia Mutated) activation. We hypothesize
that HZE stimulates the Alt-NHEJ pathway.

February 13-16, 2024 875
Poster Session B: SR CVD and other Degenerative Tissues

361 Nina Porter; Brendon Bagley; Robert Rigor; Bradley Williams
(1625760) CASE REPORT: EARLY ACUTE RADIATION-INDUCED CARDIOMYOPATHY
AFTER FRACTIONATED X-RAY IRRADIATION WITHIN PARAMETERS OF A MISSION TO
MARS: A PORCINE MODEL
Rapid decompensation from acute heart failure syndrome presumed secondary to radiation-
induced cardiomyopathy/myocarditis in a healthy young adult pig highlights a potential risk to
astronauts from prolonged mission exposure to galactic cosmic radiation.
362 Timothy Boorady; Ashleigh Brown; Marvin Coombs; Carl Gibbins; Diane Gonzales; Mai
Nguyen; Brendon Bagley; Andrew Garcia; Amory Koch; Antoinette McNeary-Garvin;
Alexander Pattin; Liem Phan; Nina Porter; Maura Rabbette; Robert Rigor; Fabiola Rivera;
Kevin Sunderland; Bradley Williams
(1644632) PERSISTENT LYMPHOCYTE REDUCTION AFTER FRACTIONATED X-RAY
IRRADIATION WITHIN SPACE MISSION TO MARS PARAMETERS: A PORCINE MODEL
In order to replicate chronic exposure to galactic cosmic radiation, six Yucatan minipigs were
monitored and exposed to cranial-caudal directed X-ray radiation doses over 8 weeks,
ranging from 0.125Gy to 0.5Gy/week.
363 Karra Johnson; Aiyi Lui; Ella Tamgue; Ashley Blackwell; Richard Britten
(1647289) THE EFFECTS OF SPACE RADIATION ON THE ESTROUS CYCLE OF
FEMALE RATS
This abstract describes the findings from a study done to test the effects of space radiation
on the estrous cycle in female rats.
364 Alexandra Stibler; Michelle Meyers; Alexis Mraz; Laura Bowman; Catherine Davis; Anthony
Lau
(1648649) MECHANICAL PROPERTY CHANGES IN BONE EXPOSED TO GALACTIC
COSMIC RADIATION
This study investigates the effects of galactic cosmic radiation (GCR) on the mechanical
properties of bone via a rat model. 3-pt bend testing was conducted to evaluate how dose
rate and time post-exposure affect bone strength. Significant sex differences and a general
trend of decreasing stiffness and maximum load with time was demonstrated. The aim is to
help prolonged space travel become safer.
365 Gabriel Gifford; Marjan Boerma; Xiao Mao; Ashley Nemec-Bakk; Vijayalakshmi Sridharan
(1648698) MYOCARDIAL CHANGES IN A MOUSE MODEL OF PROLONGED
SPACEFLIGHT
This study is on stress-induced myocardial changes from prolonged spaceflight in a mouse
model using samples collected from mice that have lived thirty-five days aboard the
international space station.
366 Panagiota Perdikis; Ali Stibler; Michelle Meyers; Laura Bowman; Alexis Mraz; Dale Johnson;
Catherine Davis; Anthony Lau
(1649190) BONE MATERIAL STRENGTH CHANGES IN RATS EXPOSED TO ACUTE
PROTON RADIATION
This study focused on the effects acute proton radiation has on the material properties of rat
bone strength. Micro indentation testing was used to get information on the viscoelastic
material properties of the cortical bone. This was helpful in getting information about how
space radiation affects the material properties of astronaut's bones.

February 13-16, 2024 885
395 Nicholas Cavallero; Anthony Lau; Catherine Davis; Alexis Mraz; Laura Bowman
(1649505) MICROSTRUCTURAL CHANGES IN BONE EXPOSED TO GALACTIC COSMIC
RADIATION
This study analyzes the effect of galactic cosmic radiation (GCR) on bone microstructural
strength. Finite element analysis techniques were implemented to perform simulated axial
compression on rat bone models. Factors of sex, time point, and exposure rate were
analyzed to assess changes in bone microstructure. Results demonstrated sex-specific
trends with females having more variability.
396 David Goukassian; Agnieszka Brojakowska; Siras Hakobyan; Suren Davitavyan; Ani
Stepanyan; Mary Khlgatian; Malik BIsserier; Shihong Zhang; Gisane Khachatryan; Tamara
Sirunyan; Venkata Naga Garikipati; Gohar Tsakanova; Arsen Arakelyan
(1649604) EVALUATION OF MOSAIC LOSS OF Y CHROMOSOME AND Y-LINKED GENE
MUTATIONS AFTER EXPOSURE TO GAMMA AND SIMGCRSIM RADIATION DURING
LIFESPAN OF MALE C57BL6/J MICE
An improved understanding of IR-mediated mLOY is necessary to advance determination of
individual susceptibility and provide an early opportunity for development of personalized
mitigation of a wide spectrum of human diseases including cancer, neuro- and
cardiovascular diseases.
397 David Goukassian; Agnieszka Brojakowska; Malik Bisserier; Siras Hakobyan; Suren
Davitavyan; Ani Stepanyan; Mary Khlgatian; Shihong Zhang; Arsen Arakelyan
(1649685) RADIATION-INDUCED CLONAL HEMATOPOIESIS AS AN INDEPENDENT
RISK FACTOR FOR CARCINOGENESIS AND HEART DISEASE DEVELOPMENT
The results of this studies provide scientific evidence on whether gamma and/or space type
IR exposure can promote long-term genomic instability in the hematopoietic system with
“long-range” effects in the heart and various internal organs.
398 Joo Hyun Kim; Khaled Kamal; Mariam Othman; John Ford; Nancy Turner; John Lawler
(1650175) MITIGATING SPACE RADIATION-INDUCED CARDIAC DAMAGE THROUGH
FISH OIL AND PECTIN SUPPLEMENTATION
Mitigating Space Radiation-Induced Cardiac Damage through Fish Oil and Pectin
Supplementation
399 Sylvia Natividad-Diaz; Binata Joddar; Wilson Poon; Aibhlin Esparza; Andie Padilla; Mario
Mata Corral
(1658869) 3D MICROFLUIDIC CARDIAC MODEL IN MICROGRAVITY
Our team will engage in a pilot project to assess and understand the potential of a Human 3D
Microfluidic Cardiovascular tissue model with risks imposed by environmental stressors
including radiation, weightlessness, and evaluate mitigation strategies enabled by medical
nanotechnologies contributing to NASA astronaut mission performance and health
outcomes.
400 Steven Lin; Nicholas Palaskas; Sivareddy Kotla; Gregory Gladish; Jun-ichi Abe; Swamique
Yusuf; Penny Fang; Bouthaina Dabaja; Caroline Chung
(1704035) IMAGING AND SERUM BIOMARKERS TO PREDICT AND IDENTIFY EARLY
CARDIAC INJURY FROM RADIATION EXPOSURE
In otherwise healthy, low cardiovascular risk cancer patients receiving radiotherapy for
esophageal, lung, and lymphoma malignancies have minimal early longitudinal changes in
echocardiographic parameters in the months after chest radiation above 6 Gy dose, with only
a trend for a larger decline in left ventricular ejection fraction in the photon compared to the
proton treated patients.

February 13-16, 2024 895
Poster Session B: SR Risk Modeling, Mitigation, Partnerships, and Initiatives

342 Shelita Augustus; Robin Elgart
(1643694) OPTIMIZING SAMPLE COLLECTION AND ACCESSIBILITY THROUGH THE
BIOSPECIMEN AND TISSUE SHARING AND COLLECTION (BTSC) PROGRAM
This abstract discusses Space Radiation Element's Biospecimen and Tissue Sharing and
Collection (BTSC) Program.
343 Shirin Rahmanian; Floriane Poignant; Tony Slaba
(1646319) A GENERALIZED MICRODOSIMETRIC DOSE RESPONSE MODEL
A microdosimetric dose response model is presented based on distribution of energy
depositions and number of ion traversal inside cell nuclei. Simulation with the track-structure
code RITRACKS were used to develop parametric functions to describe different populations
of cell nuclei. The model is applied to various experimental data sets and compared to
various known dose response models.
344 Chirayu Patel; Kaitlyn Reno; Alexander Borg; Deborah Snyder; Paula Bennett; Ashley
Nemec-Bakk; Charles Pearson; Michael Sivertz; Adam Rusek; Peter Guida; Igor Koturbash;
Marjan Boerma; Jeffery Chancellor; Jeffrey Willey
(1646790) PROLONGED LOW-DOSE RATE IRRADIATION OF BOTH TAIL SUSPENDED
AND FULL WEIGHT BEARING MICE OVER 24H USING SIMULATED GALACTIC COSMIC
RAYS
This study has helped the NSRL develop the capability and protocol to perform protracted
irradiation of mice while also exposed to combined hazards, specifically with hind limb
unloading.
345 Nousha Afshari; Marjan Boerma; Jeffrey Willey; Jeff Chancelllor
(1648336) CHALLENGING THE CAUSE OF NON-MALIGNANT IONIZING RADIATION
EFFECTS VIA NUMERICAL METHODS
This project investigates the impact non-nuclear subcellular targets have on the occurrence
of non-malignant ionizing radiation effects.
346 Kirill Grigorev; Ana Uriarte Acuna; Lauren Sanders; Danielle Lopez; Ryan Scott; Samrawit
Gebre; Jack Miller; Livio Narici; Sylvain Costes
(1649178) RADLAB: GRAPHICAL AND PROGRAMMING INTERFACES FOR
INTERROGATION OF SPACE TELEMETRY DATA
The RadLab project is aimed at connecting dosimetry data from multiple radiation detectors,
currently focused on the ones located on the ISS, and with the goal to establish a self-
sustained portal receiving continuous updates from multiple databases and individual
investigator contributions; RadLab implements a graphical and an application programming
interface for streamlined access to the data.
347 Premkumar Saganti; Mahamudur Rahman; Huichen Wang; Solange Massa
(1649229) ASSESSMENT OF CUSTOM BUILT NANO COMPOSITE MATERIALS FOR
RADIATION SHEIDING EFFECTIVENESS
New materials without lead to shield harmful radiation are being developed by our
collaborative partner, Ecoatoms, an Aerospace Biomanufacturing Company, Reno, NV. We
tested their custom build samples to assess the effectiveness of those materials for x-ray
shielding. Our results are promising with the samples and biological assessment. We present
our preliminary results.

February 13-16, 2024 905
348 Jeff Chancellor; Nousha Afshari; Marjan Boerma; Carlos Granja; Igor Koturbash; Wayne
Newhauser; Jeffrey Willey; Jaqueline Williams; Megan Chancellor
(1649612) EMULATION OF THE SPACE RADIATION ENVIRONMENT FOR MATERIALS
TESTING AND RADIOBIOLOGICAL EXPERIMENTS
Here we show that the linear energy transfer (LET) spectrum of the intravehicular
environment of spaceflight vehicles can be reproduced at a terrestrial accelerator laboratory
by impinging a monoenergetic beam of ions of a single species upon a passive scattering
apparatus.
349 Robert Hinshaw; Sylvain Costes; Marianne Sowa; Egle Cekanaviciute
(1649675) R3 REAL-TIME RADIATION RESPONSE: GENETICALLY ENGINEERED
CELLULAR SENSORS FOR BIOLOGICAL EFFECTS OF SPACEFLIGHT
Here we present plans for a newly funded initiative leveraging genetic engineering for
endogenous sensors of radiation damage and other self-reporting datapoints to enable
automation of data collection for biology experiments beyond low Earth orbit.

February 13-16, 2024 915
Poster Session B: Team

310 Daniel Newton; Jeff LePine; Ned Wellman
(1643033) RIDING THE ENGAGEMENT CASCADE: EFFECTS OF TASK SEQUENCES ON
ENGAGEMENT AND PERFORMANCE
We examine how the sequencing of task meaningfulness affects overall daily engagement
and performance.
311 Projna Paromita; Alaa Khader; Sydney Begerowski; Suzanne Bell; Theodora Chaspari
(1644272) A LINGUISTIC ANALYSIS ON THE IMPACT OF TEAM INTERACTIONS ON
TEAM PERFORMANCE DURING SPACE EXPLORATION MISSIONS
Studying the impact of team interaction and subtle communication on team performance
degradation through linguistic analysis of real-time conversation among team members.
312 Lacey Davis; Barrett Caldwell
(1644844) METRICS FOR COORDINATION: DISTRIBUTED TEAM PERFORMANCE AND
INFORMATION EXCHANGE AMIDST TIME-DELAYS AND EMERGENT EVENTS
Concepts for task coordination and effective team communication amidst time-delays and
emergent events are developed; in addition, we describe an Extended Reality (XR)
application for training crew and flight controllers for servicing, assembly, and maintenance
tasks in simulated space operating environments.
313 Megan Chan; Noshir Contractor; Leslie DeChurch
(1646391) PRISM: PERFORMANCE REIMAGINED IN SPACEFLIGHT MULTITEAM
SYSTEMS
This study contributes to understanding performance in Spaceflight Multiteam Systems
(SFMTSs) by: 1) using optimization to measure performance within and across individual,
team, SFMTS levels, 2) utilizing temporal network models to identify global relational
predictors of performance, and 3) applying social-influence network models to identify
individual relational predictors of performance.
314 Noshir Contractor; Alina Lungeanu; Leslie DeChurch; Suzanne Bell; Megan Chan; Anoop
Javalagi
(1646488) TEAMSTAR: SUPPORTING RESILIENT TEAMS TO GO THE DISTANCE
This study presents a newly developed dashboard that can be used by space crews and
ground support both before and during space missions.
315 Anoop Javalagi; Alina Lungeanu; Leslie DeChurch; Noshir Contractor
(1648323) TIME-SERIES ANALYSES OF TEAM PROCESSES IN ISOLATED SPACE
CREWS
The current study leverages longitudinal data and employs time-series to investigate a set of
team process variables—identification, action process, viability, implicit coordination, and
status conflict—in isolated crews over time to make recommendations as to which team
processes: (1) show meaningful variation over time, (2) relate to other team states.
316 Ulubilge Ulusoy; Garrett Reisman
(1648759) HUMAN FACTORS RESPECT FOR HUMAN AUTONOMY TEAMING IN DEEP
SPACE HABITATS
We offer a new construct called “Human Factors (HF) Respect” to investigate humans’
willingness to assist their autonomous agent teammates and to quantify the ways in which
humans assist autonomous agents in the context of deep-space habitat operations.

February 13-16, 2024 925
317 Lila Berger; Eduardo Salas

(1649400) TEAMWORK SKILL DECAY IN LONG DURATION SPACEFLIGHT
Due to the significant disparities between current and future spaceflight missions, the existing
teamwork training framework may be insufficient, requiring the development of preventative
measures and potential interventions for teamwork skill decay, as well as a more
comprehensive understanding of the quality of teamwork training astronauts receive prior to
launch.
318 Andres Käosaar; Krisztina Szabó; C. Shawn Burke
(1649428) STARS ALIGNING OR COLLIDING? CULTURAL DIVERSITY IN SPACE
ANALOG CREWS
The comparison between the two SIRIUS space analog crews on cultural diversity and its
effects on team dynamics is presented.
319 Susannah Aronson; Laura Ikuma
(1649742) BRINGING NASA TEAMS EVALUATIONS DOWN TO EARTH
This study proposes a new approach to NASA crew research - using college students in an
escape room to simulate NASA team interactions.
320 Aleksandra Stankovic; Stijn Thoolen; Eric Olsen; Katie Harris; Lacey Davis; Barrett Caldwell;
Stephen Robinson; Gary Strangman
(1650903) TEAM PROBLEM-SOLVING SKILLS FOR LUNAR EXPLORATION
This project represents an operationally-relevant assessment of team problem-solving in the
context of upcoming Cis-Lunar exploration, with the ultimate goal of contributing towards the
establishment of future autonomous team-based concepts of operations and functional
requirements for system design, operations, and training.

February 13-16, 2024 935
Thursday, February 15, 2024
Behavioral Health in Rodent Models After Exposure to Spaceflight Hazards
Chairs: Amber Paul and Ajit Mulavara

9:00 AM Amber Paul; Angela Cebula; Marissa Burke; Erik Larsen; Kaylin Juarez; Carol Mitchell;
Cassandra Juran; Candice Tahimic; Stephani Tabares Ruiz; Janani Iyer; Stephanie Puukila;
Siddhita Mhatre; Joshua Alwood; Moniece Lowe; Linda Rubinstein; Yasaman Shirazi-Fard;
Marianne Sowa; April Ronca
(1650802) IMMUNE MOBILIZATION CIRCADIAN PATTERNS IN THE SPACEFLIGHT
ENVIRONMENT
In spaceflight, circadian rhythms are misaligned, along with immune dysregulation. Yet, the
relationship between circadian patterns and immunity have not been fully characterized in
the spaceflight environment. To this end, we utilized a ground, based model of spaceflight to
analyze the impact of diurnal time on immune mobilization patterns.
9:15 AM Larry Sanford; Laurie Wellman; Zachery Luyo; Riley Heerbrandt; Alea Boden; Namrata
Singh; Richard Britten
(1643541) SEX DIFFERENCES IN THE EFFECTS OF SPACE RADIATION AND SOCIAL
ISOLATION ON SLEEP AND EEG SPECTRA IN RATS
Social isolation differentially impacts sleep and EEG spectra in male and female rats.
9:30 AM Brian Kangas; Amaya Jenkins; Janet Baulch; Charles Limoli; Rajeev Desai
(1649625) GALACTIC COSMIC RADIATION AND SLEEP FRAGMENTATION PRODUCE
DEFICITS ON SOME, BUT NOT ALL, COGNITIVE PROCESSES IN MICE
To assist NASA’s spaceflight risk estimation, studies were conducted to examine the impact
of GCR exposure or sleep disruption in mice engaged in a variety of complex cognition-
related tasks designed to measure distinct complex behavioral processes.
9:45 AM Richard Britten; Ella Tamgue; Max Tenenbaum; Larry Sanford; Hargsoon Yoon; Karra
Johnson
(1646969) ESTABLISHING THE IMPACT OF SLEEP DISRUPTIONS ON THE DEFAULT
MODE NETWORK SUPPRESSION UNDER COGNITIVE LOADING.
Sleep is known to impact the suppression of the Default Mode Network, we have developed
a technique to identify spectral changes accompanying the DMN suppression and will
eventually determine if sleep-induced DMN suppression is related to loss of cognitive
performance.
10:00 AM Isabel Yu; Evan Smith; Monica Dawes; Emily Burke; Eleana Solakidou; Xiaoyu Ma; Brian
Kangas; Alexandros Makriyannis; Subhamoy Chatterjee; Maher Dayeh; Mihir Desai; Charles
Limoli; Rajeev Desai
(1649802) COMPLEX 33-BEAM SIMULATED GALACTIC COSMIC RADIATION
EXPOSURE IMPACTS VENTRAL TEGMENTAL AREA NEUROTRANSMITTER
NETWORKS IN MALE MICE
We examined the effects of acute and chronic galactic cosmic radiation exposure on
neurochemistry in the ventral tegmental area in mice, and found minimal changes to
dopamine, serotonin, norepinephrine, glutamate, and GABA in contrast to our past work on
neurochemistry in the prefrontal cortex.
10:15 AM Discussion
10:30 AM Break

February 13-16, 2024 945
Masters of Metabolism for Long Duration Spaceflight
9:00 AM Galleon
Chair: Gary Strangman

9:05 AM Christopher Porada; Jonathan Diaz; James Rice; Ritu Ramamurthy; Alan Jacobson; Thomas
Shupe; Anthony Atala; Paul Wilson; Graça Almeida-Porada
(1649743) USING HUMAN ORGANOIDS AND FOSSILIZED REMAINS FROM EXTINCT
HOMININS TO UNLOCK THE SECRETS OF TORPOR/HIBERNATION
In the present studies, we are using human organoids and fossilized remains from extinct
Sima de los Huesos Hominins to unlock the secrets of torpor/hibernation and assess the
ability of this state to provide protection from space radiation
9:22 AM Tammy Chang; Marie Dicker; Juan Reyna; Anthony Consiglio; Brenda Ametepe; Radhika
Narain; Alan Maida; William Galloway; Yun Weng; Boris Rubinsky
(1664043) ISOCHORIC SUPERCOOLING OF LIVER ORGANOIDS AND WHOLE LIVER
ORGANS
Liver organoids and whole liver organs are used as models to examine the effect of isochoric
supercooling on metabolic function.
9:39 AM Allyson Hindle; Dylan Barth; Thomas Crippen; Raizel Yankaway; Zachary Carlson; Jenifer
Utz; Frank van Breuekelen
(1642524) BENEFITS AND TRADEOFFS OF WARM TORPOR IN A MODEL MAMMALIAN
HIBERNATOR
Gene expression signatures and body temperature dynamics in a model hibernating rodent
indicate that hibernation at intermediate and warm temperatures invoke the greatest changes
in whole-animal and cell-level physiology. These data will inform human cell culture studies
investigating tradeoffs between metabolic depression and cell dysregulation across different
target temperatures of hibernation.
9:56 AM Katharyn Flickinger; Alexandra Weissman; Andrea Johnsson; Ryann DeMaio; Victor Wu;
Jonathan Birabaharan; Philip Empey; Daniel Buysse; Francis Guyette; Clifton Callaway
(1626332) NOVEL METHOD OF METABOLIC MANIPULATION USING SUBCUTANEOUS
INFUSION OF DEXMEDETOMIDINE
We recruited healthy volunteers to test if an oral loading dose followed by a 6-hour
subcutaneous infusion of dexmedetomidine can reduce metabolism.
10:13 AM Discussion
10:30 AM Break

February 13-16, 2024 955
IMPACT Health Risk Assessment Tool
Chair: William Thompson

9:00 AM Ashley Nelson; William Thompson
(1664544) IMPACT, A TOOL SUITE FOR CREW HEALTH AND PERFORMANCE SYSTEM
TRADE ANALYSES AND DECISION SUPPORT- TRANSITION TO OPERATIONS
This presentation will update the audience on the development status of the IMPACT tool
suite as it comes out of its System Acceptance Review (SAR) and nears Transition to
Operations (TTO).
9:15 AM Prashant Parmar; Arian Anderson; Dave Hilmers; Eric Kerstman; Dana Levin; Ariana Nelson;
Shean Phelps; Jon Steller
(1645107) IMPROVING QUANTIFICATION OF MEDICAL EVACUATION RISK FOR
HUMAN SPACEFLIGHT IN THE IMPACT MODEL
An analysis and update of the Return to Definitive Care (RTDC) metric of IMPACT.
9:30 AM Arian Anderson; David Hilmers; Eric Kerstman; Ariana Nelson; Shean Phelps; Jon Steller
(1647399) OVERLAPPING CONDITIONS IN IMPACT
The IMPACT Evidence Library is composed of 119 clinical conditions that are modeled using
probabilistic risk assessment to Identify risk metrics and inform medical system design. This
paper reviews the technical process of refining and improving the list of medical conditions
used within the model to reduce how overlapping conditions may reduce model fidelity.
9:45 AM Lynn Boley; John Arellano; David Hilmers; Eric Kerstman; Prashant Parmar; Gina Vega;
Millennia Young
(1648333) IMPACT REAL WORLD SYSTEM VALIDATION
A validation analysis of ExMC's updated decision support tool known as IMPACT (Informing
Mission Planning via Analysis of Complex Tradespaces) was performed with respect to a set
of International Space Station (ISS) and Shuttle Transportation System (STS) real world
system (RWS) referent data due to the limited referent data available from exploration
missions.
10:00 AM Jon Steller; Anderson Arian; David Hilmers; Dana Levin; Ariana Nelson; Prashant Parmar;
Shean Phelps; Emily Stratton
(1649632) IMPROVING THE FIDELITY OF CAPABILITY & RESOURCE WEIGHTING IN A
PROBALISTIC RISK ASSESSMENT MODEL FOR SPACEFLIGHT
Incorporating a partial weighting methodology of medical system resources in lieu of the all-
or-nothing treatment assumption provides a more accurate and operationally-relevant
representation of medical risk without compromising IMPACT’s ability to inform overarching
medical system requirements.
10:15 AM Discussion
10:30 AM Break

February 13-16, 2024 965
Characterizing and Mitigating Exploration Mission Stressors
Chairs: George Brainard and Alexandra Whitmire

9:00 AM Rachel Jansen; Erin Flynn-Evans; Zachary Glaros
(1649951) IMPACT OF SLEEP SHIFTING STRATEGIES ON THE INTERNATIONAL
SPACE STATION
We aim to determine which strategies yield the best sleep outcomes and to determine
whether the sleep shifting strategy has consequences to subsequent sleep in crewmembers
aboard the ISS.
9:13 AM Erin Flynn-Evans; Rachel Jansen; Alissa Braun; Zachary Glaros; Scott Smith; Sara Zwart
(1649824) CAFFEINE CONSUMPTION ABOARD THE INTERNATIONAL SPACE STATION
This study explores how astronauts use caffeine and how it affects their sleep.
9:26 AM George Brainard; George Brainard; John Hanifin; Mijail Serruya; Benjamin Warfield;
Taehwan Yoo; John Kemp; Shadab Rahman; Toni Clark; Melissa St. Hilaire; Robert Soler;
Daniel Garcia; Kelly Norwood; James Maida; Smith Johnston; Ronald Moomaw; Laura
Barger; Charles Czeisler; Steven Lockley
(1646444) TESTING SOLID STATE LIGHTING COUNTERMEASURES TO IMPROVE
CIRCADIAN ADAPTATION, SLEEP, AND PERFORMANCE DURING HIGH FIDELITY
ANALOG AND FLIGHT STUDIES FOR THE INTERNATIONAL SPACE STATION (ISS)
This study is examining solid-state lighting countermeasures to enhance circadian
adaptation, sleep quality, and performance in high-fidelity analog and flight studies
conducted aboard the International Space Station (ISS).
9:39 AM Lauren Landon; Suzanne Bell; Princess Dickson
(1646451) INTEGRATED NASA AND PRIVATE ASTRONAUT CREWS READINESS
NEEDS ASSESSMENT
We summarize results of a technical interchange meeting (TIM) focused on knowledge gaps
and countermeasure needs for integrated crews of NASA astronauts and private astronauts
on the ISS.
9:52 AM Tripp Driskell; C Burke; James Driskell; Andres Kaosaar; Krisztina Szabo
(1642831) THE HUMAN SIDE OF LONG-DURATION SPACE MISSIONS: AN
EXAMINATION OF ASTRONAUTS' INTERPERSONAL EXPERIENCES
In this research, we conduct a content analysis on interviews from 20 former astronauts to
gain insight into the development and maintenance of interpersonal relations among
spaceflight crews.
10:05 AM Dorothy Carter; Joshua Pearman; Alina Lungeanu; Marissa Shuffler; Leslie DeChurch;
Noshir Contractor; Aaron Schecter; Lauren Landon; Jacob Pendergraft; Emily Gerkin; Laura
Bauer; Caton Weinberger
(1648777) PROJECT FUSION: THE IMPACT OF COMMUNICATION DELAY ON
PROBLEM-SOLVING CONNECTIVITY IN SPACEFLIGHT MULTITEAM SYSTEMS
We will present results of our experiments conducted as part of the Human Exploration
Research Analog (HERA) Campaigns 5 and 6 which show that communication delay
significantly decreases the ability of spaceflight multiteam systems to jointly problem-solve in
real time.
10:18 AM Discussion
10:30 AM Break

February 13-16, 2024 975
Sensorimotor: Parabolic Flight
Chairs: Scott Wood and Timothy Macaulay

9:00 AM Timothy Macaulay; Austin Bollinger; Scott Wood; Millard Reschke; Gilles Clement
(1644475) FUNCTIONAL TASK TESTS IN PARTIAL GRAVITY DURING PARABOLIC
FLIGHT
This study presents data on the performance of functional tasks (such as standing, walking,
and jumping) during the partial gravity phases of parabolic flight (0.25g, 0.5g, and 0.75g)
compared to during steady flight (1.0g).
9:15 AM Mark Shelhamer; Nabila Ali; Jacob Lefler; Mallika Sarma; Michael Schubert
(1638372) INVESTIGATION OF PARTIAL-G EFFECTS ON OCULAR ALIGNMENT
In parabolic flight, we find evidence for a g threshold in alignment of the eyes (a reflection of
central neural compensation for otolith asymmetry).
9:30 AM Ana Diaz Artiles; Nathan Keller; Madison Weinrich; Renee Abbott; Traver Wright; Bonnie
Dunbar; Deanna Kennedy
(1647654) BIMANUAL COORDINATION DURING PARTIAL GRAVITY IN PARABOLIC
FLIGHT: PRELIMINARY RESULTS
Twelve subjects participated in a parabolic flight campaign where we investigated bimanual
coordination performance in partial gravity (0g, 0.25g, 0.50g, and 0.75g). In this presentation,
we will provide an overview of the experiment and discuss our initial parabolic flight results.
9:45 AM Vladimir Ivkovic; Stijn Thoolen; JoAnna Pollonais; Bryan White; Guillaume Spielmann; Quan
Zhang; Gary Strangman
(1650647) OPEN-PG: OPERATIONAL PERFORMANCE EFFECTS AND
NEUROPHYSIOLOGY IN PARTIAL GRAVITY - PRELIMINARY POST FLIGHT SUMMARY
Successful data collection has been conducted in baseline and parabolic flight conditions,
with preliminary data analyses suggesting acquisition of high-quality physiological,
behavioral, and operational performance data.
10:00 AM Sarah Piechowski; Daniel Aeschbach; Peter Gauger; Christian Mühl; Maximilian Thiemann
(1648024) SIMULATED MANUAL SPACECRAFT CONTROL UNDER THE INFLUENCE OF
DIFFERENT GRAVITY CONDITIONS DURING A PARABOLIC FLIGHT
Manual spacecraft control performance during parabolic flights decreased in microgravity,
but not during hypergravity, suggesting a higher relevance of impaired spatial orientation due
to confounding vestibular signals compared to the mere change in force vectors.
10:15 AM Discussion
10:30 AM Break

February 13-16, 2024 985
Sex Differences in Biological Effects from Radiation Exposure
Chairs: Stuart Lee and Catherine Davis

1:15 PM David Goukassian; Agnieszka Brojakowska; Siras Hakobyan; Suren Davitavyan; Ani
Stepanyan; Mary Khlgatian; Malik Bisserier; Shihong Zhang; Lahouaria Hadri; Venkata Naga
Garikipati; Raj Kishore; Arsen Arakelyan
(1649724) FEMALE AND MALE HEARTS RESPOND DIFFERENTLY TO SAME DOSES OF
GAMMA AND SIMGCRSIM RADIATION: SEX MATTERS
There are significant and long-lasting IR-induced functional and structural alteration in the LV
function in male but not female mice, that were associated with upregulation of chloride
transmembrane transport and cytokine activity. In male LVs exposed to simGCRsim
upregulation of a set of ribosomal genes suggests an additional sex-specific and IR-specific
responses in male but not female mice.
1:30 PM Candice Tahimic; Mollie Brekker; Maya Semel; Julia Santos; Ivan Korostenskij; Kaelyn Kelly;
Osanna Krikourian; Ioannis Palatsidis; Stephanie Puukila; Siddhita Mhatre; Janani Iyer;
Amber Paul; Joshua Alwood; Yasaman Shirazi-Fard; Linda Rubinstein; Steffy Tabares Ruiz;
Moniece Lowe; Michael Delp; David Goukasian; April Ronca
(1648714) CARDIOVASCULAR RESPONSES TO SIMULATED SPACEFLIGHT:
MOLECULAR SIGNATURES AND SURROGATE OUTPUTS TO MEASURE CVD RISK
Study aims to determine the effects of simulated space radiation and microgavity on
molecular signatures of cardiovascular health.
1:45 PM Megan Evans; Eunbee Park; Heather Doviak; Yohei Arai; Yuka Arai; Soichi Sano; Karen
Hirschi; Francine Garrett-Bakelman; David Goukassian; Kenneth Walsh
(1650245) THE PATHOLOGICAL CONSEQUENCES OF MOSAIC LOSS OF THE Y
CHROMOSOME SECONDARY TO IONIZING RADIATION ARE COMPLETELY
UNKNOWN.
Mosaic loss of the Y chromosome in blood shows limited expansion following exposure to
gamma radiation.
2:00 PM Cynthia Lemere
(1650348) SEX- AND APOE-SPECIFIC LATE CNS EFFECTS OF SPACE RADIATION AND
A NATURAL HISTORY STUDY OF BIOMARKERS IN A HOMOLOGOUS SPECIES
This presentation will provide a broad summary of our NASA-funded studies over the past 5
years, including in vivo, in vitro and biomarker projects.
2:15 PM M. Kerry O'Banion; Mark Osabutey; Laura Calvi; John Olschowka; Tanzy Love; Jacqueline
Williams
(1649810) EFFECT OF SPACE RADIATION ON INNATE IMMUNE SYSTEM
HOMEOSTASIS DRIVES IMMUNE AND ENDOTHELIAL CELL DYSFUNCTION AND
NEURODEGENERATION
Update on current NASA project examining brain and bone marrow changes following
simplified GCRsim irradiation in C57BL/6 male and female mice.
2:30 PM Discussion
2:45 PM Break

February 13-16, 2024 995
Flight Research Operations & Integration
1:15 PM Galleon
Chair: Cherie Oubre

1:15 PM Pasha Morshedi; Lea Hougland; Stephanie Kriegor; Amanda Lauret; Shanna Rodgers;
Gwenn Sandoz; Monica Sheth; Cherie Oubre
(1652012) RESEARCH OPERATIONS AND INTEGRATION FLIGHT SESSION: ALWAYS
SWEAT THE SMALL STUFF
Interactive overview of the Research Operations and Integration activities during spaceflight
data collection sessions
2:15 PM Discussion
2:45 PM Break

February 13-16, 2024 1005
Analysis & Risk Approaches To Exploration Spaceflight Health
Chair: Kris Lehnhardt

1:15 PM Hunter Rehm; Caroline Austin; Mona Matar; Jerry Myers
(1626292) HUMAN SYSTEMS RISK NETWORK INDEPENDENT RANKING ANALYSIS
We present a novel method for ranking each human system risk by its progression potential
using the HSRB DAGs and graph theoretic techniques.
1:30 PM Lauren McIntyre; Sean Bostic; Clara Gasiewski; Drayton Munster; Jerry Myers
(1628860) MEDICAL RESOURCE SET BULKY ITEM TRADE SPACE ANALYSIS FOR
SPACEFLIGHT MEDICAL RISK
This talk will present results showing the quantitative impact of forced inclusion of several
bulky items across a variety of medical kit constraints, and the effect that a potential research
investment into reducing the bulky item mass and volume may have on risk.
1:45 PM Jerry Myers; Suleyman Gokoglu; Beth Lewandowski; Mona Matar; Lauren McIntyre; Drayton
Munster
(1628876) CHP-PRA PROOF-OF-CONCEPT SENSITIVITY ASSESSMENT
To further understand the relative changes in the CHP probabilistic modeled component
risks, and to elucidate how future refinements can be targeted in such modeling, a means of
establishing the contributions of component risk contributors will be demonstrated.
2:00 PM Amanda Smith; Dennis Beaugrand; Melissa Lyons; Michele Beaugrand; Philip Augustine;
Luis Montalvo
(1644828) CREW HEALTH AND PERFORMANCE INTEGRATED DATA ARCHITECTURE
PROJECT UPDATES
The Crew Health and Performance Integrated Data Architecture (CHP-IDA) is a platform
being developed to integrate CHP data and provide analytics and decision support systems
during future Exploration missions. This presentation will address project updates, including
increased CHP domain engagement, recent architecture developments, new integrations,
and scenarios in which CHP-IDA would be used.
2:15 PM Erik Antonsen; Ahmed Abukmail; John Arellano; Kevin Huynh; Robert Reynolds; Mary Van
Baalen
(1662663) NETWORK ANALYTIC APPROACHES FOR HSRB DAG DEVELOPMENT
Overview of network analytic approaches for DAGs and application of initial centrality
measures analysis to the Renal Stone Risk.
2:30 PM Discussion
2:45 PM Break

February 13-16, 2024 1015
Brain and Behavioral Health After Exposure to Spaceflight Hazards – Results from Ground Analogs and Spaceflight
Chairs: Vonetta Dotson and Rachael Seidler

1:15 PM Donna Roberts; James Taylor; Joe Tidwell; Heather Collins
(1652976) ASSOCIATIONS BETWEEN WINSCAT PERFORMANCE AND LOCAL PRE- TO
POSTFLIGHT CHANGES IN BRAIN STRUCTURE
Statistically significant associations were noted between post-flight structural changes in
three brain regions (supplemental motor area, left hippocampus, and right pallidum) and pre-
to inflight performance changes on two WinSCAT tests (CPT and CDS), suggesting further
studies are warranted to assess for any potential causal relationships between these
variables.
1:30 PM Rashika Rao; Sutton Richmond; Rayn Ramclam; Swati Rane; Dawn Kernagis; Kody
Coleman; Mehmet Albayram; Jens Rosenberg; Jeffrey Iliff; Rachael Seidler
(1642170) EFFECTS OF ELEVATED CO2 ON GLYMPHATIC FUNCTION
Here, we assessed the effect of breathing elevated carbon dioxide on human glymphatic
clearance.
1:45 PM Peter zu Eulenburg; Nicholas Ashton; Kaj Blennow; Judith-Irina Buchheim; Galina Vassilieva;
Henrik Zetterberg; Alexander Choukèr
(1643186) BRAIN HEALTH OBSERVATIONS FROM BLOOD-BASED BIOMARKERS IN
LONG-DURATION SPACEFLIGHT AND SPACE ANALOG ENVIRONMENTS
Brain-structural integrity is affected due to long-duration spaceflight but not in space analog
environments.
2:00 PM Alex Stahn; Simone Kühn; David Roalf; Ruben Gur; Christopher Jones; Makayla Cordoza;
Marc Kaizi-Lutu; Yoni Gilad; Suzanne Bell; Brad Nindl; Brian Martin; Warren Bilker; David
Dinges; Mathias Basner
(1646424) NSCOR FOR ADAPTATION AND RESILIENCE: BRAIN STRUCTURAL
CHANGES IN RESPONSE TO ISOLATION AND CONFINEMENT
This work summarizes first findings of brain structural changes in response to different ICE
and ICC analogs that were collected as part of the NSCOR Adaptation and Resilience.
2:15 PM Michael Funke; Sandra Pusil; Bader Shirah; Ricardo Bruna; Pablo Cuesta; Christopher
Laohathai; Ana Maria Cebolla; Jens Haueisen; Guy Cheron; Patrique Fiedler; Fernando
Maestu
(1648732) A CASE STUDY OF EEG CONNECTIVITY IN SHORT- AND LONG-DURATION
LOWER EARTH ORBIT SPACEFLIGHT MISSIONS
The EEG derived Default Mode Network alpha band power differences between long- and
short-duration LEO flights warrant further investigation with a standardized EEG recording
protocol, warranting further research into adaptability mechanisms and mitigation strategies
as potential neurophysiological markers.
2:30 PM Discussion
2:45 PM Break

February 13-16, 2024 1025
Advances in Food Nutrition, Acceptability, and Microbiology for Spaceflight
Chairs: Grace Douglas and Mark Ott

1:15 PM ChiHeng Wu; Takiyah Sirmons; Grace Douglas; Danielle Froio-Blumsack; Millennia Young
(1641858) IMPROVEMENT OF SHELF LIFE FOR SPACE FOOD THROUGH HURDLE
APPROACH
This study investigated the potential to extend the shelf life of foods using a hurdle approach
of combinations of different processing technologies, formulations, packaging, and lower
temperature storage. Data to date indicates it is essential to store 16 of the 33 foods at 4°C
to extend its shelf life beyond 3 years due to the decrease in either acceptability, nutrients, or
both at 21°C.
1:30 PM Starla Thornhill; Lindsey Papineau; C. Ott
(1642738) MICROBIAL SURVIVAL IN BREWED TEA
Loose-leaf bagged tea comprises 7% of the astronaut food system but often has a high
microbial load; this study investigated the effects of water brewing temperature (boiling, ISS
PWD, and ambient temperature water) on microbial survival in brewed tea over a 2 two-hour
drinking window.
1:45 PM* Grace Douglas; Thomas Oswald; Suzanne Bell; Millennia Young
(1652571) FOOD ACCEPTABILITY, MENU FATIGUE, AND AVERSION ON ISS MISSIONS
Characterizing the relationship between food acceptability and mission duration will
contribute to defining requirements for an acceptable food system that will support crew
health and performance on long duration missions.
1:45 PM* Grace Douglas; Thomas Oswald; Suzanne Bell; Millennia Young
(1652579) FOOD ACCEPTABILITY, MENU FATIGUE, AND AVERSION DURING THE
SIRIUS-21 ANALOG MISSION
Characterizing the relationship between food acceptability and mission duration will
contribute to defining requirements for an acceptable food system that will support crew
health and performance on long duration missions.
2:05 PM Jess Bunchek; Mary Hummerick; Carolina Franco; Gioia Massa
(1643508) NUTRITION OF ANTARCTIC-GROWN CROPS TO SUPPLEMENT THE CREW
DIET, WITH APPLICATIONS FOR SPACEFLIGHT
Pick-and-eat leafy greens and fruiting crops that were grown in the EDEN ISS plant
cultivation facility in Antarctica have been analyzed for nutrients of interest, especially as
supplements to the astronaut crew diet during longer-duration spaceflight missions.
2:20 PM Mary Hummerick; Victoria Castro; Jennifer Gooden; Christina Khodadad; Gioia Massa; Mark
Ott; Cherie Oubre; Cory Spern
(1643727) DATA CONSOLIDATION FOR THE INTERNATIONAL SPACE STATION'S
ENVIRONMENTAL AND VEGGIE MICROBIAL ISOLATES INCREASES TRENDING
CAPABILITIES
A microbial data base was developed to compile and standardize the microbiological data
from environmental sampling and Veggie flight samples enabling a tool to analyze the data
for microbial trends among bacterial and fungal isolation.
2:35 PM Discussion
2:45 PM Break

February 13-16, 2024 1035
Advanced Biological and Mathematical Techniques to Understanding Space Radiation Damage
Chairs: Ryan Norman and Lisa Simonsen

3:00 PM Stephen Kunkel; Michael Cornforth
(1645596) IS THERE ANY DIFFERENCE BETWEEN CHROMOSOME DAMAGE INDUCED
BY LOW- VS HIGH-LET RADIATION AT THE DNA LEVEL?
Cytogenomic analysis of cells exposed to high- versus low-LET radiation reveals an
increasing number of breakpoints per chromosome aberration but no difference in the
molecular signature of those breakpoints.
3:15 PM Floriane Poignant; Steve Blattnig; Sylvain Costes; Janice Huff; Tony Slaba; Ianik Plante
(1642088) PREDICTING CELL SURVIVAL, CHROMOSOME ABERRATIONS AND THEIR
TRANSMISSIBILITY
This work presents a model of cell survival together with a model of chromosome aberrations
combined to calculate the yield of stable chromosome aberrations.
3:30 PM Eric Wang; Igor Shuryaky; David Brenner
(1638809) QUANTIFYING THE EFFECTS OF NEUTRON DOSE, DOSE PROTRACTION,
AGE AND SEX ON MOUSE SURVIVAL, USING PARAMETRIC REGRESSION AND
MACHINE LEARNING ON A 21,000-MOUSE DATA SET
Using advanced machine learning techniques to analyze data from 21,308 neutron-irradiated
mice, our study revealed a nuanced dose-response relationship for mortality risk influenced
by variables like dose, age, and sex, offering critical insights for radiation protection in
contexts like space travel and cancer therapy.
3:45 PM Viviana Risca; Hera Canaj; Andrew Scortea; Devany West; Justin Rendleman; Ianik Plante
(1649349) EPIGENETIC STATE MODULATION OF RADIATION-INDUCED DNA DAMAGE
We present an update describing further analysis of RICC-seq DNA fragment mapping from
our ion irradiation of human fibroblasts and new results from low-LET pilot experiments
assayed for single-strand DNA break and double-strand DNA break densities using GLOE-
seq and END-seq.
3:55 PM Ianik Plante; Viviana Risca; Devany West
(1619480) SIMULATION OF RADIATION-INDUCED DNA DAMAGE SHOWS HISTONE
PROTECTION
The simulation of radiation-induced DNA damage has been done with code RITRACKS for
several ions. To perform this simulation, the ionization, dissociative attachment and elastic
collision cross sections have been updated. The simulation results show a reduction in DNA
damage when histones are present.
4:05 PM Rebecca Arian; Hala Awada; Minjun Kim; Estrella Passerat de la Chapelle; Wonjae Lee;
François Paris; Egle Cekanaviciute; Sylvain Costes
(1646211) 53BP1 AS A BIOSENSOR TO TRACK IONIZING RADIATION-INDUCED DNA
DAMAGE AND REPAIR USING LIVE-CELL IMAGING
I am working to transduce U251-COVGT5 cells with a 53BP1-GFP plasmid in order to track
53BP1 protein expression and innate immune response upon irradiation, thereby creating a
live-cell imaging system.
4:15 PM Discussion
4:30 PM Break

February 13-16, 2024 1045
Isolation Analogs
3:00 PM Galleon
Chair: Sara Whiting

3:00 PM Sara Whiting; Bryan Caldwell; Christina Johnson
(1648989) THINKING BIG AND BROAD ABOUT ISOLATION AND CONFINEMENT
ANALOGS: A COMMUNITY PANEL DISCUSSION
Panel discussion among isolation analog operators and PIs.
3:05 PM Discussion
4:30 PM Break

February 13-16, 2024 1055
Long Term Health Metrics & Risks for Exploration Spaceflight Health
Chair: Bara Reyna

3:00 PM Benjamin Easter; Deb Goodenow; William Thompson; David Hilmers; Jonathan Steller; Dana
Levin; Arian Anderson; Emily Stratton; Ariana Nelson; Eric Kerstman; Lynn Boley; John
Lemery; Kris Lehnhardt
(1655065) MEDICAL RISK ESTIMATES AND CLINICAL CAPABILITY NEEDS FOR A
LONG DURATION ARTEMIS MISSION
This presentation will present estimates for medical risk and clinical capability needs for an
extended Artemis mission.
3:15 PM Beth Lewandowski; Christopher Gallo; Matthew Prelich; Megan Sickinger; Hunter Rehm;
Mona Matar; Suleyman Gokoglu; Ashley Nelson; Lauren McIntyre; Jerry Myers; Jacqueline
Charvat; Jessica Keune; Mary VanBaalen
(1622751) LONG-TERM HEALTH METRIC DEVELOPMENT EFFORT
Development efforts for astronaut long-term health metrics for incorporation into a crew
health and performance probabilistic risk assessment.
3:30 PM Matthew Prelich; Christopher Gallo; Suleyman Gokoglu; Beth Lewandowski; Mona Matar;
Lauren McIntyre; Jerry Myers; Hunter Rehm; Megan Sickinger
(1630780) ASSESSING LONG-TERM HEALTH OUTCOMES IN ASTRONAUTS
This analysis aims to elucidate long-term health effects on astronauts through statistical and
machine learning modeling by investigating life expectancy differences between astronauts
and the general population, extracting possible correlations between number of spaceflight
hours flown and increased mortality,and analyzing life expectancy outcomes from the subset
of astronauts who died of cancer.
3:45 PM Benjamin Easter; David Hilmers; Lynn Boley; Shean Phelps; Eric Kerstman; Debra
Goodenow; William Thompson; Ali Al; Shane Schwartz; Kris Lehnhardt
(1655091) PRELIMINARY MEDICAL RISK ESTIMATES AND CLINICAL CAPABILITY
NEEDS FOR ARTEMIS IV
This presentation will provide preliminary estimates of medical risk and clinical capabilities
from the IMPACT tool for the Artemis IV mission.
4:00 PM Audrie Colorado; Brian Crucian; Stephanie Krieger; Gailen Marshall; Torin Mccoy; Mayra
Nelman-Gonzalez
(1660927) HAZARDS OF LUNAR SURFACE EXPLORATION: DETERMINING THE
IMMUNOGENICITY/ALLERGENICITY OF LUNAR DUST
This in-vitro investigation indicates no detectable immediate reactivity/hypersensitivity
between human peripheral leukocytes and lunar dust.
4:15 PM Discussion
4:30 PM Break

February 13-16, 2024 1065
Human Capabilities Assessment for Autonomous Missions: VNSCOR Outbrief
Chairs: Stephen Robinson and Andrew Beitman

3:00 PM Stephen Robinson
(1673039) 2024 UPDATE FOR THE VIRTUAL NASA SPECIALIZED CENTER OF
RESEARCH (VNSCOR) - HUMAN CAPABILITIES ASSESSMENTS FOR AUTONOMOUS
MISSIONS (HCAAM)
Annual research findings of the seven-team “HUMAN CAPABILITIES ASSESSMENTS FOR
AUTONOMOUS MISSIONS” (HCAAM) project.
3:03 PM Jessica Marquez; Jimin Zheng; Shivang Shelat; John Karasinski; John Bresina
(1645476) CREW AUTONOMY THROUGH SELF-SCHEDULING: GUIDELINES FOR
CREW SCHEDULING PERFORMANCE ENVELOPE AND MITIGATION STRATEGIES
Update on results from self-scheduling investigation within HERA Campaign 6.
3:18 PM Christine Fanchiang; Katya Arquilla; David Klaus; Mark Shelhamer; Michael Zero
(1648237) HCAAM: USING A HUMAN CAPABILITIES FRAMEWORK TO QUANTIFY
CREW TASK PERFORMANCE IN HUMAN-ROBOTIC SYSTEMS-YEAR 5
Updates to investigation of using wearables for validating a human capabilities framework.
3:33 PM Daniel Selva; Prachi Dutta; Poonampreet Josan; Bonnie Dunbar; Raymond Wong; Ana Diaz
Artiles
(1649336) VIRTUAL ASSISTANT FOR ANOMALY RESOLUTION IN LONG-DURATION
EXPLORATION MISSIONS: OVERVIEW OF RESULTS SO FAR AND NEXT STEPS
We provide an update on the status of an on-going project to study the effect of using Virtual
Assistants to support crew members in anomaly resolution during Long Duration Exploration
Missions. We compare the results of a HERA Campaign 6 study with those of a similar lab
experiment, and report on the results of a second lab experiment focusing on the effect of
agent accuracy and explanations.
3:48 PM John Lee; Ernest Cross; James Garrett; Morgan Klaeser; Vianney Renata
(1661760) MANAGING AND MEASURING TRUST DIFFUSION ACROSS TEAM
MEMBERS
The efficacy of this measurement and management depends on going beyond the individual
to consider team interactions to identify key nodes and communication patterns that
influence trust dynamics that can be used to enhance trust or temper overtrust.
4:03 PM Debra Schreckenghost; Kritina Holden; Tod Milam; I. W. T. Robertson
(1644296) ENHANCING SITUATION AWARENESS OF AUTOMATED PROCEDURES
USING ADAPTIVE MULTIMODAL AUGMENTED REALITY DISPLAYS
The Virtual Intelligent Task Assistant (VITA) project investigates the effects on human
performance of a virtual task assistant that combines procedure automation with augmented
reality multi-modal user interfaces.
4:18 PM Discussion
4:30 PM Break

February 13-16, 2024 1075
Micro & Immune
Chairs: Mark Ott and Brian Crucian

3:00 PM Satish Mehta; Rahul Suresh; Keith Brandt; Douglas Diak; Scott Smith; Sara Zwart; Grace
Douglas; Mayra Nelman-Gonzalez; Simon Clemett; Tyson Brunstetter; Brian Crucian
(1647044) IMMUNE SYSTEM DYSREGULATION PRECEDING A CASE OF
LABORATORY-CONFIRMED ZOSTER/DERMATITIS ONBOARD THE INTERNATIONAL
SPACE STATION
We report a case of laboratory confirmed herpes zoster (shingles) in an astronaut during an
approximately six-month spaceflight onboard International Space Station. Immune markers,
including peripheral leukocyte distribution, T cell function, plasma cytokine, stress hormones,
and latent herpesvirus reactivation are reported.
3:15 PM Honglu Wu; Adriana Babiak-Vazquez; Brian Crucian; Liang-Hao Ding; Stephanie Krieger;
Maria Moreno-Villanueva; Ye Zhang
(1649481) TRANSCRIPTOMIC ANALYSIS OF ISS CREWMEMBERS PERIPHERAL
BLOOD MONONUCLEAR CELLS REVEALS HOMEOSTATIC REGULATIONS IN SPACE
Blood from eleven ISS crewmembers was collected before, during and after long duration
space missions. Transcriptomic analysis was performed in isolated peripheral blood
mononuclear cells (PBMCs) using the RNA-sequencing technique, revealing homeostatic
mechanisms in space.
3:30 PM Matthew Wargo; Kristin Schutz; Atul Chander; Kasthuri Venkateswaran; Nitin Singh
(1650253) VIRULENCE AND TRANSCRIPTIONAL RESPONSES OF AN ISS KLEBSIELLA
PNEUMONIAE ISOLATE TO LOW-SHEAR SIMULATED MICROGRAVITY
Characterization of virulence and molecular responses of three Klebsiella pneumoniae
strains to low shear simulated microgravity
3:45 PM Mark Ott; Jennifer Barrila; Sara Koroli; Sandhya Gangaraju; Richard Davis; Starla Thornhill;
Jiseon Yang; Kristyn Hoffman; Rebecca Rice; Audrie Medina-Colorado; Christian Vu; Bianca
Kang; Laura Banken; Phillip Stafford; Cherie Oubre; Brian Crucian; Cheryl Nickerson
(1647561) SPACEFLIGHT-INDUCED CHANGES IN MICROBIAL VIRULENCE AND THE
IMPACT TO THE HOST IMMUNE RESPONSE
This study investigates the phenotypic and transcriptomic responses of five bacterial
pathogens to better understand the breadth of medically significant microbial pathogens that
exhibit altered virulence and pathogenesis-related responses when cultured in spaceflight
analogue conditions.
4:00 PM Norberto Gonzalez-Juarbe; Amanda Appel; Aji Mary Taal; Dominique Moser; Judith-Irina
Buchheim; Rosana Wiscovitch-Russo; Harinder Singh; Manolito Torralba; Lorenzi Hernan;
Alexander Choukér
(1650539) THE IMPACT OF HYPOBARIC HYPOXIA AND CONFINEMENT STRESS ON
HOST-MICROBIOME INTERACTION, RESULTS FROM THE CHOICE STUDY
In this ground-based proposal we use a multi-omic approach to address the impact of long-
term (~1 year) hypobaric hypoxia and confinement on the human gut microbiome, intestinal
immunity and systemic inflammation.
4:15 PM Sergey Ponomarev; Olga Kutko; Marina Rykova; Vjacheslav Shmarov
(1646073) INFLUENCE OF 240-DAY ISOLATION IN A HERMETIC FACILITY WITH
ARTIFICIAL HABITAT (PROJECT "SIRIUS-21") ON THE STATE OF THE HUMAN IMMUNE
SYSTEM
During the 8-month isolation experiment, several notable changes were observed in the
human immune system which illustrate the significant impact of extended isolation on various
components of the immune system.
4:30 PM Break
February 13-16, 2024 1085
Friday, February 16, 2024
Space Radiation Central Nervous System Decrements
Chairs: Gregory Nelson and Ajit Mulavara

8:30 AM Elliot Smits; Richard Britten; Faith Reid
(1645562) WILL SPACE RADIATION EXPOSURE LEAD TO ALTERED RISK-TAKING
BEHAVIOR?
This study suggests that SR-induced loss of executive function performance may not be
confined to just cognitive functions but may also affect impulsivity and mood-regulating
executive functions. Furthermore, this study raises the possibility that SR exposure may also
impact numerosity skills, reward evaluation, and anterograde interference.
8:45 AM Laura Bowman; Rosalie Connell; Catherine Davis; Anthony Lau; Michelle Meyers; Alexis
Mraz; Patricia Thomas
(1648056) THE CORRELATION BETWEEN BONE AND NEUROBEHAVIORAL CHANGES
IN RATS EXPOSED TO HELIUM-4 RADIATION
Investigates a potential link between bone and memory changes following exposure to
Helium-4 radiation and a novel method for identifying subjects in irradiated groups who did
not respond to radiation.
9:00 AM Jacob Raber; Sarah Holden; Kat Kessler; Breanna Glaeser; Chloe McQuesten; Mitali
Chaudhari; Fiona Stenzel; Marek Lenarczyk; Scott Leonard; Jeffrey Morre; Jaewoo Choi;
Amy Kronenberg; Alexander Borg; Andy Kwok; Jan Stevens; Christopher Olsen; Jeffrey
Willey; Gerd Bobe; Jessica Minnier; John Baker
(1645679) EFFECTS OF PHOTON AND MIXED BEAM IRRADIATION IN THE PRESENCE
AND ABSENCE OF HINDLIMB UNLOADING ON BEHAVIORAL AND COGNITIVE
PERFORMANCE AND METABOLIC PATHWAYS IN PLASMA OF FISCHER 344 RATS
Male Fischer 344 rats were irradiated with photons or with a 5-ion 6 beam GCR simulator
exposure in the absence or presence of simulated weightlessness achieved using hindlimb
unloading (HU) to identify potential plasma biomarkers of photon radiation exposure or HU
condition for behavioral or cognitive performance; there were 30 plasma metabolites related
to the 13 behavioral measures.
9:20 AM Prashant Nagpal; Sadhana Sharma; Vincenzo Gilberto; Sydney Risen; Breonna Kusick;
Christina Fallgren; Michael Weil; Stephanie McGrath; Julie Moreno; Anushree Chatterjee
(1642821) HIGH-THROUGHPUT TARGET IDENTIFICATION TO VALIDATION AND
TRANSLATION OF LEAD NANOLIGOMER NEUROTHERAPEUTIC RADIATION
COUNTERMEASURE
In this talk, we describe our results in rapid high-throughput target screening, hit-to-lead
molecule optimization, lead validation in radiation model, followed by safety-tox studies in
large and small animals, towards clinical translation of lead Nanoligomer neurotherapeutic as
an effective radiation neurological countermeasure
9:40 AM Ashley Blackwell; Richard Britten; Christine Kim; Jovanna Tracz
(1648683) DEEP SPACE RADIATION AND SLEEP FRAGMENTATION
DISRUPT FINE MOTOR CONTROL IN FEMALE RATS
Space radiation (SR: He or GCRsim) differentially impacted fine motor control in female rats
3 mo post-exposure. After fragmented sleep (FS), postural stability and nose movement
changed in all rats. GCRsim-exposed rats also had more mouth misses than He. This is the
first study to demonstrate impaired fine motor control and postural stability after exposure to
SR and FS in female rats.
9:55 AM Discussion
10:00 AM Break

February 13-16, 2024 1095
Space Biology: Plants in Space
8:30 AM Galleon
Chairs: Sharmila Bhattacharya

8:30 AM Gioia Massa; Mary Hummerick; Christina Khodadad; LaShelle Spencer; Raymond Wheeler;
Anirudha Dixit; Cory Spern; Millennia Young; Grace Douglas
(1647244) VEG-05 TOMATO CROP TESTING ON THE INTERNATIONAL SPACE
STATION
A summary of the tomato crop testing conducted on the ISS in 2022-2023.
8:50 AM Mark Sperry; Lawrence Koss; Blaine Fitzgerald; Anirudha Dixit; Cory Spern; Raymond
Wheeler; Zachary Lippman; Christopher Dardick
(1647386) EVALUATING CANDIDATE BIOTECH FRUIT CROPS FOR SPACEFLIGHT
SUITABILITY
This research aims to conduct preliminary testing on biotech fruits optimized for controlled
environments by utilizing NASA’s Environmental Test Chamber System (ETCS) to identify
biotech crops that positively respond to typical spaceflight conditions.
9:10 AM Kellie Walters; Ethan Darby; Sarah Armstrong
(1649579) BRASSICA CARINATA FOR SPACE PRODUCTION: CUT-AND-COME AGAIN
PROTOCOLS FOR YIELD AND PHYTONUTRIENT OPTIMIZATION
The objective was to determine the optimal harvest interval and number of consecutive
harvests of mustard green Brassica carinata ‘Green Amara’ to maximize yield and
phytonutrients when grown under International Space Station (ISS)-like conditions.
9:30 AM Annie Shelton; Chris Dardick; Neil Mattson
(1650123) HORTICULTURE ADVANCEMENTS FOR DWARF PLUM (PRUNUS
DOMESTICA L) AS A POSSIBLE SPACEFLIGHT CROP CANDIDATE
Advancing spaceflight technical readiness for over-expressed FT1 Plum (Prunus domestica
L) through the development of a hydroponic cultivation methodology and investigation of Far
Red on fruit production.
9:50 AM Discussion
10:00 AM Break

February 13-16, 2024 1105
Pharmacy, Therapeutics & Metabolism in Exploration Class Missions
Chairs: John Reichard and Aaron Allcorn

8:30 AM John Reichard
(1640937) A QUANTITATIVE ASSESSMENT OF DRUG STABILITY TO IONIZING
RADIATION.
The effect of ionizing radiation (IR) on drugs provisioned for exploration space missions is
uncertain. To better understand this uncertainty, over 1000 dose -response measurements
were collected for 136 IR-exposed drugs from 68 reports. The quantitative analysis of these
data will be presented and discussed.
8:45 AM Lindsay Woodard; Melissa Wright; Kelsey Broderick; Eric Shuler; Sara McBride; Ryan
Carter; Seth Stewart; Jeff Chancellor
(1648849) PACKAGING FOR IMPROVED PHARMACEUTICAL PROTECTION IN SPACE
Protective packaging solutions for pharmaceuticals onboard the spacecraft were developed
and evaluated.
9:00 AM Emily Stratton; Sarah Lumpkins
(1660949) CONCEPT OF OPERATIONS FOR THE PREVENTION, DIAGNOSIS, AND
TREATMENT OF RENAL STONES FOR MARS MISSIONS
The creation of a Concept of Operations for the prevention, diagnosis, and treatment of renal
stones for Mars missions.
9:15 AM George Pantalos; Charles Elder Elder; Jonathan Kopechek; Michael Menze; Thomas
Roussel; Sienna Shacklette; Brett Janis
(1646294) PRESERVED RED BLOOD CELLS FOR TRANSFUSION THERAPY IN
REDUCED GRAVITY
Dehydrated red blood cells are shelf stable at ambient temperature for several years and can
be easily rehydrated for transfusion therapy using a standard infusion line set.
9:30 AM John Reichard; Veneese Brown; Amanda Buerger; Ernest Fung; Andrew Maier; Andrey
Massarsky; Keegan Rogers; Lisa Yang
(1696615) A FRAMEWORK APPROACH FOR EVALUATION OF POTENTIAL
DEGRADATION OF ACTIVE PHARMACEUTICAL INGREDIENTS FOR LONG-DURATION
SPACEFLIGHTS
The aim of this project is to develop an assessment framework to identify likely active
pharmaceutical ingredient (API) degradants formed under spaceflight conditions and to
evaluate the potential health risks associated with exposures to such degradants.
9:45 AM Discussion
10:00 AM Break

February 13-16, 2024 1115
Human Factors Considerations for Future Missions
Chairs: Jessica Marquez and Debra Schreckenghost

8:30 AM Hannah Weiss; Leia Stirling
(1648315) AUGMENTED REALITY-BASED NEUROVESTIBULAR ASSESSMENTS FOR
FUTURE LONG-DURATION MISSIONS
The use of augmented reality (AR) may provide a lightweight, space-conscious solution to
inflight neurovestibular assessments. In this study, the performance of healthy adults on six
dynamic tasks was evaluated within AR and while using traditional physical objects to
examines how virtually administered holographic balance tasks impact users’ postural control
and task completion strategies.
8:43 AM Kelden Ben-Ora; Katherine Homer; John Karasinski; Jessica Marquez; Stephen Robinson
(1649179) HUMAN CAPABILITIES ASSESSMENTS FOR AUTONOMOUS MISSIONS
(HCAAM) RESEARCH UPDATE: ENABLING AUTONOMOUS CREW TASK
PERFORMANCE WITH MULTIMODAL ELECTRONIC PROCEDURE
COUNTERMEASURES
Two enhanced procedure tools have been developed for a complex mechanical repair task
analogous to a spacecraft repair procedure utilizing suites of sensors and tablet-based real-
time augmented reality visuals to increase crew autonomy and reduce the need for
communication with mission control during future long-duration human spaceflight missions.
8:56 AM Ian Robertson; Vanessa Jones; Shu-chieh Wu; Megan Parisi; Kaitlin McTigue; Tina
Panontin; Ryan Amick; John Karasinski; Mark Cramer; Kevin Arango; Brett Montoya; Andrew
Fladboe
(1647530) AN EVALUATION OF THE POTENTIAL OF EXTENDED REALITY
TECHNOLOGIES FOR VERIFICATION TESTING AT NASA
In this presentation, we will present the work we conducted to evaluate XR technologies for
use in verification tests at NASA.
9:09 AM Amanda Smith; Kritina Holden; Ian Robertson; Shu-Chieh Wu; Megan Parisi; Katie McTigue;
Vanessa Jones
(1638365) INTERFACE CONSISTENCY PHASE I
This presentation will discuss work completed during Phase I, including the definition of
consistency, a taxonomy by which it is applied to user interfaces, the development of
methods used to assess consistency, and results from a simple pilot study.
9:22 AM Michelle Lin; Lauren Landon; Katya Arquilla
(1642812) FACTORS IMPACTING HABITABILITY: ANALYSIS OF SHAQ DATA FROM
HERA C5 AND C6
The purpose of the current project is to extend the prior work by exploring data from HERA
C6 and from additional behavioral health measures collected in HERA C5/C6.
9:35 AM Donna Dempsey; Robert Sargent; Kara Latorella; Alan Hobbs; Ruthan Lewis; Chrisopher
Niemann
(1648180) ASSESSING NUMBER OF CREW FOR MARS AGAINST TRADE SPACE
PARAMETERS
We present a methodology for trade space analysis to consider the number of crew
necessary to accomplish missions to Mars. We include the results of three human-
performance models developed using the Improved Performance Research and Integration
Tool (IMPRINT) modeling platform and results of a fourth custom-built model on expertise
within the crew that support the trade space analysis.
9:48 AM Discussion
10:00 AM Break
February 13-16, 2024 1125
Musculoskeletal Health and EVA Performance
Chairs: Patrick Estep and Brian Prejean

8:30 AM Overview Remarks
8:45 AM Anna Wadhwa; Jennifer Coulombe; Marie Mortreux; Shannon Emerzian; Daniel Brooks;
Jackson Hanlon; Jarred Chow; Jason Ciola; Dorothy Hu; Shawn Berry; Francesca Gori;
Roland Baron; Jeffrey Willey; Charles Fuller; Satoru Takahashi; Martha Hotz-Vitaterna; Mary
Bouxsein
(1644401) RESPONSE OF THE MURINE SKELETON TO ARTIFICIAL GRAVITY INDUCED
BY CENTRIFUGATION ON THE INTERNATIONAL SPACE STATION: A NASA-JAXA
COLLABORATION
Artificial gravity induced by centrifugation onboard the ISS (using the JAXA Multiple Artificial-
gravity Research System [MARS]) partially mitigated the deleterious effects of spaceflight on
the murine skeletal system, though there was no clear dose-dependent response.
9:00 AM Marie Mortreux; Dong-Min Sung; Janice Nagy; Jennifer Coulombe; Jason Ciola; Anna
Wadhwa; Shannon Emerzian; Seward Rutkove; Jeffrey Willey; Charles Fuller; Martha Hotz-
Vitaterna; Satoru Takahashi; Mary Bouxsein
(1642065) ARTIFICIAL GRAVITY INDUCED BY CENTRIFUGATION ON THE
INTERNATIONAL SPACE STATION PARTIALLY MITIGATES MUSCLE ATROPHY IN
MICE
This abstract discusses how artificial gravity provided by centrifugation onboard the
International Space Station, impacts muscle health after a 30-day mission in orbit. We
present longitudinal data acquired pre and post flight, alongside some new impedance data
to better understand the potency of artificial gravity as an in-flight countermeasure for muscle
deconditioning.
9:15 AM Jeffrey Willey; Chirayu Patel; Kaitlyn Reno; Rae Young Leslie Kim; Jennifer Coulombe;
Charles Fuller; Satoru Takahashi; Martha Hotz Vitaterna; Mary Bouxsein
(1643092) ARTIFICIAL GRAVITY DURING SPACEFLIGHT PREVENTS GAIT AND
PERFORMANCE DEFICITS IN A GRAVITY-DOSE DEPENDENT MANNER
Continuous exposure to 1g of artificial gravity generated via centrifugation by the JAXA
Multiple Artificial-gravity Research System prevented gait and performance deficits in a
gravity-dose dependent manner in mice upon return from the ISS, with continuous exposure
to µg yielding mice non-functional; 0.33g (Mars gravity) resulting in pronounced gait deficits;
and 0.67g attenuating these deficits.
9:30 AM Logan Kluis; Callie Wynn; Deanna Kennedy; Ana Diaz-Artiles
(1647108) UTILIZING MACHINE LEARNING TO PREDICT METABOLIC COST WITH
VARYING SPEEDS, INCLINES, AND GRAVITY LEVELS
Our research aims to quantify the adverse effects of spacesuits on human performance via
modeling metabolic cost.
9:45 AM Jason Norcross; Taylor Schlotman; Lauren Cox; Richard Rhodes; Nicole Strock; Millennia
Young; Karina Marshall-Goebel
(1651959) VALIDATION OF FITNESS FOR DUTY STANDARDS USING PRE- AND POST-
FLIGHT CAPSULE EGRESS AND SUITED FUNCTIONAL PERFORMANCE TASKS IN
SIMULATED REDUCED GRAVITY - 2024
Egress Fitness is part of the CIPHER protocol and is evaluating the capability and
performance of space flight deconditioned subjects when asked to perform emergency
capsule egress tasks with return to earth or initial EVA tasks upon landing on Mars.
10:00 AM Break

February 13-16, 2024 1135
Biological Effects of Combined Stressors
Chairs: Janice Huff and Jeffrey Willey

10:15 AM Igor Koturbash; Kirsten Clements; Vijayalakshmi Mohan Seenivasan; Ashley Nemec-Bakk;
Wayne Newhauser; Chirayu Patel; Jeffrey Willey; Jackie Williams; Marjan Boerma; Jeffrey
Chancellor
(1647048) COMBINATION OF GALACTIC COSMIC RADIATION EXPOSURE AND
SIMULATED MICROGRAVITY RESULT IN LATE PRO-INFLAMMATORY RESPONSES
AND EPIGENTIC REPROGRAMMING IN THE MOUSE LUNG
We demonstrate persistence of biological responses in the lung tissue of mail mouse as a
result of combined exposure to galactic cosmic rays and and simulated microgravity.
10:30 AM John Baker; Marek Lenarczyk; Ammar Alsheikh; Andy Kwok; Alexander Borg; Jeffrey Willey;
Amy Kronenberg
(1644926) EXPOSURE TO GALACTIC COSMIC RADIATION CAUSES CARDIAC
PERIVASCULAR FIBROSIS AND SIMULATED WEIGHTLESSNESS ENGAGES THE
IMMUNE SYSTEM: COMBINED EXPOSURE DOES NOT CAUSE SYNERGY IN MATURE
RATS
Galactic cosmic radiation causes cardiac perivascular fibrosis and microgravity engages the
immune system with no synergy in astronaut aged rats.
10:45 AM Ashley Nemec-Bakk; Kim Krager; Nukhet Aykin-Burns; Marjan Boerma
(1643851) EFFECTS OF CHRONIC LOW DOSE RATE IONIZING RADIATION AND
SIMULATED MICROGRAVITY ON THE CARDIOVASCULAR SYSTEM
This study utilizes a new ground based model to determine the cardiovascular effects of
extended chronic low-dose rate ionizing radiation exposure and simulated microgravity in
mice.
11:00 AM Susan Bailey; Lynn Taylor; Audrie Colorado; Mayra Nelman; Sara Bustos-Lopez; Cody
Gutierrez; Brian Crucian; Kerry George
(1643042) TELOMERES 2 - CIPHER
For the Complement of Integrated Protocols for Human Exploration Research (CIPHER), our
goal is to establish temporal profiles of telomere length and DDRs in a larger and more
diverse population of astronauts assigned to missions of various durations, and in ground
control subjects of spaceflight analog studies (prolonged isolation) to further test and
strengthen significance of results.
11:15 AM Ioannis Michalopoulos; Polina Malatesta; Konstantinos Kyriakidis; Megumi Hada; Hiroko
Ikeda; Akihisa Takahashi; Premkumar Saganti; Alexandros Georgakilas
(1648038) DIFFERENTIAL GENE EXPRESSION IN HUMAN FIBROBLASTS
SIMULTANEOUSLY EXPOSED TO PARTIAL GRAVITY AND LOW DOSE-RATE
NEUTRONS
Over-expressed genes upon neutron radiation of human fibroblasts under simulated earth's
gravity are mainly involved in the mitochondrial electron transport chain, and under-
expressed genes code ribosomal proteins, while few genes, mainly involved in the
cytoskeleton, were differentially expressed upon sham-radiation under simulated partial
gravity or hypergravity and earth's gravity
11:30 AM Discussion
11:45 AM Break

February 13-16, 2024 1145
How We Do Business
10:15 AM Galleon
Chairs: Jancy McPhee and Erik Hougland

10:15 AM Kim Lowe; David Francisco; Imene Mechkene
(1647442) NASA SPACEFLIGHT HUMAN-SYSTEM STANDARD MAINTENANCE: AN
EVOLVING STRATEGY TO KEEP NASA AGENCY-LEVEL STANDARDS CURRENT
THROUGH PARTNERSHIPS WITH THE SCIENTIFIC COMMUNITY
The NASA-STD-3001 documents establish guidelines that are consistent with the most up-
to-date evidence and recommendations, which is accomplished through active collaboration
with the scientific community and stakeholders.
10:35 AM Wilma Anton; Laurie Abadie; Erik Hougland; Lisa Milstead; Baraquiel Reyna
(1644738) MANAGING HRP'S EXTERNAL DELIVERABLES: THE PROCESS AND THE
CUSTOMERS
This presentation describes HRP's coordinated process for ensuring the successful transfer
of research external deliverables (EDs) to HRP's customers and provides an overview of the
nature of different types of EDs and how they impact the customers’ products in support of
risk mitigation for future NASA exploration missions.
10:50 AM Mary Kirby; Vanessa Lehman
(1645408) THE HUMAN RESEARCH PROGRAM GRANT LIFECYCLE AND
ADMINISTRATION
We will share the key tenants of the grant life cycle from formulation to closeout with the
intent to provide key information to Principal Investigators (PI) on how to be successful
throughout the process.
11:05 AM Jennifer Ensley Gorshe; Marisa Covington
(1644121) DOES THIS ACTIVITY NEED IRB OVERSIGHT?
In this session, attendees learn IRB staff's process and criteria for determining whether an
activity constitutes *research* with *human subjects* (which necessitates IRB oversight); or
conversely, what activities might be considered Not Human Subjects Research (NHSR), for
which IRB oversight is not required.
11:20 AM Sara Asgar; Marisa Covington
(1646636) CRAFTING CLARITY: MASTERING THE ART OF INFORMED CONSENT FORM
WRITING
This session intends to provide suggestions to researchers on how to write a succinct
consent form that effectively conveys information regarding their study to potential
participants.
11:35 AM Discussion
11:45 AM Break

February 13-16, 2024 1155
Exploration Crew Health and Performance
Chair: Dave Hilmers

10:15 AM Mona Matar; Suleyman Gokoglu; Caroline Austin; Lauren McIntyre; Hunter Rehm; Jerry
Myers
(1629256) STRATEGIES FOR QUANTIFYING HUMAN SPACEFLIGHT PERFORMANCE IN
THE CREW HEA LTH AND PERFORMANCE SYSTEM
We suggest strategies to quantify astronaut performance specific to spaceflight activities and
illustrate how such concepts may help in optimizing the Crew Health and Performance (CHP)
system capabilities with respect to Artemis missions.
10:30 AM Beth Lewandowski; Courtney Schkurko; Kimesha Calaway; Rachael Miller; Benjamin Easter;
Rahul Suresh; Kris Lehnhardt; Moriah Thompson
(1637484) A MULTI-FACETED APPROACH TO DEMONSTRATING MULTI-FUNCTIONAL
INTEGRATED MEDICAL DEVICES TO ADVANCE EARTH-INDEPENDENT MEDICAL
OPERATIONS
Information is provided about various technology demonstrations designed to gather
evidence for or against the inclusion of multi-functional integrated medical devices within
Earth-independent medical operations systems.
10:45 AM Michael LaBarbera; Bashir El-Khoury; Shawn Joshi; Amy Kreykes; Jonathan Steller
(1647007) ASSESSING CREW MEDICAL OFFICER PREPAREDNESS FOR
INTERNATIONAL SPACE STATION MISSIONS
We present the results of a semi-structured interview-based, qualitative assessment of
astronaut crew medical officers and their preparedness to provide medical support onboard
the International Space Station, in order to inform medical preparedness for future missions
to low earth orbit, Artemis, and exploration class missions.
11:00 AM Tristan Richmond; Annie Martin
(1650492) THE HEALTH BEYOND INITIATIVE: ADVANCING MEDICAL AUTONOMY WITH
THE CONNECTED CARE MEDICAL MODULE
This abstract will describe the Health Beyond Initiative's vision for a Canadian flagship
contribution to the international space program: the Connected Care Medical Module
(C2M2).
11:15 AM Zsolt Garami; Alex Alley; Steven To; Reka Vernes; Kathleen Rosendahl-Garcia; Mark Alley
(1652191) EVALUATION OF THE EASE OF USE AND EFFECTIVENESS OF VULCAN
(VISUAL ULTRASOUND LEARNING CONTROL AND ANALYSIS NETWORK) DURING
ULTRASOUND TESTING AND TRAINING
Tienovix, LLC and Houston Methodist Hospital performed a study testing the efficiency of
Tienovix' Vulcan Software utilizing augmented reality through the Microsoft Hololens 2 as a
training tool (as opposed to traditional ultrasound training) for novice sonographers with
minimal experience.
11:30 AM Discussion
11:45 AM Break

February 13-16, 2024 1165
Measuring and Supporting Resiliency and Adaptation in Future Long Duration Exploration Missions
Chairs: Jeff Thompson and Lisa Juliette

10:15 AM Yoni Gilad; Mathias Basner; Suzanne Bell; Warren Bilker; Makayla Cordoza; Sheena Dev;
David Dinges; Ruben Gur; Takao Hensch; Christopher Jones; Marc Kaizi-Lutu; Matthew
Kayser; Lauren Landon; Brian Martin; Brad Nindle; David Roalf; Peter Roma; Alex Stahn
(1645385) NSCOR FOR EVALUATING RISK FACTORS AND BIOMARKERS FOR
ADAPTATION AND RESILIENCE TO SPACEFLIGHT: EMOTIONAL VALENCE AND
SOCIAL PROCESSES IN ICC/ICE ENVIRONMENTS
In this first presentation in the symposium, we will provide an overview of the project aims,
data collection sites, and the protocol which will be expanded upon with results from each
project aim.
10:28 AM Takao Hensch; Yuichi Makino; Gervasio Batista; Carolyn Johnson; Nathaniel Hodgson
(1650888) COGNITIVE CONSEQUENCES OF EARLY ADVERSITY DIFFER BY SEX:
IMPLICATIONS FOR LONG-TERM SPACE MISSIONS
Early adversity (ELS) leads to persistent cognitive deficits, which we find are biologically
embedded by distinct, reversible mechanisms in a mouse model of parental neglect. Our
findings inspire a nuanced consideration of adverse childhood experiences (ACEs) when
selecting/assigning crew members and offer potential mitigation strategies for overcoming
challenges of chronic space mission stress.
10:41 AM Christopher Jones; Christopher Jones; David Dinges; Mathias Basner; Alex Stahn; Brad
Nindl; Takao Hensch; Ruben Gur; Brian Martin; David Roalf; Makayla Cordoza; Warren
Bilker; Marc Kaizi-Lutu; Yoni Gilad; Sheena Dev; Suzanne Bell
(1649753) NSCOR FOR ADAPTATION AND RESILIENCE: BIOLOGICAL CORRELATES
OF RESILIENCE AND ADAPTATION
This abstract presents biomarkers of resilience identified in the NSCOR project.
10:54 AM Mathias Basner; Warren Bilker; Makayla Cordoza; David Dinges; Christopher Jones; Marc
Kaizi-Lutu; Alexander Stahn
(1648793) NSCOR FOR ADAPTATION AND RESILIENCE: DEFINING RESILIENCE WITH
THE PAIRED COMPARISON METHODOLOGY
A methodology to define resilience in ICC and ICE environments based on self-report,
objective performance, outside observations and physiologic responses is presented.
11:07 AM Lauren Landon; Alexa Doerr; Alaa Khader; Peter Roma; Suzanne Bell; David Dinges
(1644090) MEANINGFUL WORK AS A RESILIENCE COUNTERMEASURE IN EXTREME
ENVIRONMENTS
We summarize findings related to assessing meaningful work as a countermeasure to
maintain resilience in extreme environments.
11:20 AM Jeff Thompson
(1638327) NASA LEADERSHIP: PROMOTING RESILIENCE & AWE
This panel discussion provides insight behind the research examining the resilience practices
of the mental health professions responsible for the well-being of astronauts — everyone
deserves to have positive mental health and that includes our research scientists.
11:30 AM Discussion
11:45 AM Break

February 13-16, 2024 1175
Cardiovascular Responses to Varying Gravity
Chairs: Stuart Lee and Jason Lytle

10:15 AM Jason Lytle; Stuart Lee; Connor Ferguson; Stephanie Melvin; Annelise Miller; Cambria
O'Grady; Matthew Poczatek; Sondra Freeman-Perez; Steven Laurie; Millennia Young;
Brandon Macias
(1649879) EFFECT OF SEX ON ORTHOSTATIC INTOLERANCE AND
CARDIOVASCULAR RESPONSES DURING LUNAR DESCENT AND ASCENT
The purpose of this study is to determine whether there are differences between
hypovolemic men and women in orthostatic tolerance during varying simulated gravity levels
and to determine whether the Orion Orthostatic Intolerance Garment (OIG) will protect men
and women against orthostatic intolerance.
10:30 AM Jan Hönemann; Laura de Boni; Karsten Heusser; Fabian Hoffmann; Jens Jordan; Tilmann
Kramer; Stuart Lee; Stefan Moestl; Edwin Mulder; Jens Tank
(1646063) COUNTERMEASURE-SPECIFIC DISSOCIATION BETWEEN ORTHOSTATIC
TOLERANCE AND PLASMA VOLUME FOLLOWING 30 DAYS STRICT HEAD-DOWN TILT
BEDREST
The abstract comprises data on orthostatic tolerance testing and determination of plasma
volume in all groups of the SANS-CM study and will be added by findings on left ventricular
contractiliy at the meeting.
10:45 AM Stefan Möstl; Jan-Niklas Hoenemann; Fabian Hoffmann; Andre Diedrich; Karsten Heusser;
Jens Jordan; Jens Tank
(1648109) EFFECTS OF LOWER BODY NEGATIVE PRESSURE AND VENOUS THIGH
OCCLUSION ON RESTING AUTONOMIC CARDIOVASCULAR CONTROL - RESULTS
FROM THE SANS-COUNTERMEASURE STUDY
We investigated the effect of SANS-Countermeasures like LBNP and venous thigh occlusion
onto resting autonomic cardiovascular control in a 30 day strict head down tilt bed rest study.
11:00 AM Stuart Lee; Chris Miller; David Martin; Jason Lytle; Steven Laurie; Connor Ferguson;
Annelise Miller; Aaron Everson; Millennia Young; Brandon Macias
(1648634) DETERMINING DOSE RESPONSE PROFILE OF THE HEADWARD FLUID
SHIFT DURING VARYING GRAVITY LEVELS
The purpose of this study is to assess changes in vessel caliber, flow, and pressure in the
internal jugular veins (IJVs) during partial- and 0-G.
11:15 AM Ana Diaz Artiles; Richard Whittle; Nathan Keller; Eric Hall; Syeda Zaman; Hrudayavani
Vellore; Safiyya Patanam; Bonnie Dunbar
(1647047) CARDIOVASCULAR AND OCULAR GRAVITATIONAL DOSE-RESPONSE
CURVES AND EFFECTS OF COUNTERMEASURES
We have generated gravitational dose-response curves on 24 subjects (12M/12F) of a large
set of hemodynamic, autonomic, and cephalad variables during : 1) Graded tilt and 2)
Graded LBNP. We are also leveraging our experimental results to further develop
computational models that can model and predict human responses to these and other
interventions.
11:30 AM Discussion
11:45 AM Break

February 13-16, 2024 1185
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February 13-16, 2024 1195

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Redefining What’s Possible

From the Artist:

2024 NASA Human Research Program Investigators’ Workshop

NASA Human Research Program

February 13-16, 2024

Galveston Island Convention Center

Maneesh Arya, Ph.D. Kris Lehnhardt, M.D.

2024 NASA Human Research Program Investigators’ Workshop

NASA Human Research Program Investigators’ Workshop

2024 NASA Human Research Program Investigators’ Workshop

12:45 p.m. BREAK

2024 NASA Human Research Program Investigators’ Workshop

4:45 p.m. Ex Hall B/Pre-Func Area Career Development

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Space Radiation Element Welcome and Kick-off

10:45 AM Exhibit Hall B

Chairs: Janice Zawaski and Jason Weeks

10:45 AM Janice Zawaski; Jason Weeks

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Chairs: Olga Kieschnick and Kathryn Richards

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Exploration Medical Capability Element: Overview

10:45 AM Grand Ballroom A

Chair: Nancy Fleming

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Biomarkers: Central, Peripheral or Behavioral After Exposure to Spaceflight Challenges

10:45 AM Grand Ballroom B

Chairs: Catherine Davis and Jimmy Zaid

Tuesday, February 13, 2024

Sensorimotor: Space Flight and Ground Analogs

10:45 AM Grand Ballroom C

Chairs: Scott Wood and Tim Macaulay

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Beyond the Mouse (Space Radiation)…

2:45 PM Exhibit Hall B

Chairs: Steve Blattnig and Janice Zawaski

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Commercial Spaceflight and Analogs

Chairs: Emmanuel Urquieta and Jimmy Wu

2024 NASA Human Research Program Investigators’ Workshop

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Earth Independent Medical Operations (EIMO)

2:45 PM Grand Ballroom A

Chair: Jay Lemery

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

Behavioral Health and Performance: Measures and Methods

2:45 PM Grand Ballroom B

Chairs: Mathias Basner and Katherine Rahill

2024 NASA Human Research Program Investigators’ Workshop

Tuesday, February 13, 2024

SANS 1: Space Flight

2:45 PM Grand Ballroom C