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Gram Negative Rods Identification Flowchart

This flowchart summarizes the identification process for gram-negative rods found outside of stool samples. It uses a series of dichotomous keys involving lactose fermentation, oxidase testing, indole production, and colony morphology to differentiate between common pathogens such as Pseudomonas aeruginosa, Proteus species, and Escherichia coli. The end points provide likely genus identifications to guide further biochemical testing.

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1K views1 page

Gram Negative Rods Identification Flowchart

This flowchart summarizes the identification process for gram-negative rods found outside of stool samples. It uses a series of dichotomous keys involving lactose fermentation, oxidase testing, indole production, and colony morphology to differentiate between common pathogens such as Pseudomonas aeruginosa, Proteus species, and Escherichia coli. The end points provide likely genus identifications to guide further biochemical testing.

Uploaded by

Keith
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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  • Gram Negative Rods Non-Stool Pathogens Flowchart: This section provides a detailed flowchart for identifying non-stool pathogens using specific biochemical tests, starting from growth on MAC and including various tests leading to pathogen identification.

Gram Negative Rods Non-Stool Pathogens Flowchart

Growth on
MAC

Lactose Lactose
(+)* (-)*

Oxidase
Indole * Oxidase (-)*
(+)*

Colony metallic. Swarming


(-)* (+)* 1Odor of grapes
on BAP

Colony mucoid. Colony Non-mucoid


1Odor of fresh mucoid. E. coli. See No Yes No Yes
1Odor of fresh E. coli ID
bread
bread Chart
B. cepacia, P. aeruginosa Proteus
Indole*
No Yes Rare B. pseudomallei, spp.
Yes Pasteurella,
Citrobacter
or other Others (Refer to
Enteric GNR your
Enterobacter, Klebsiella, (-)* (+)*
laboratory's BT
Providencia, Rare Klebsiella, Rare rule out charts)
Citrobacter, Enterobacter Enterobacter
Cronobacter, E. coli
Others Serratia, Citrobacter,
Enterobacter, (Use
S. maltophilia, E.coli ID
Acinetobacter, Others Chart)

* = See Biochemical Tests for Gram Negative B. mallei, Y. pestis (Refer


to your laboratory's BT
Organism ID Job aid for positive and negative
rule out charts)
result reference.

1 = Note the odor without sniffing the plate.

GNR = Gram negative rods

Laboratory Training Branch

Gram Negative Rods Non-Stool Pathogens Flowchart Laboratory Training Branch Growth on MAC Lactose (+)* Indol

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