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APPENDIX B INSTALLATION

Table of Contents
Page
B.1 INSTALLING THE FILTER ASSY NO.17 (C-2).................................................. B-1
B.1.1 Pre-installation ................................................................................... B-1
B.1.2 Bubble Removal of the Filter (1)......................................................... B-1
B.1.3 Bubble Removal of the Filter (2)......................................................... B-2
B.1.4 Installation of the Filter ....................................................................... B-3
B.1.5 Bubble Removal inside Filter.............................................................. B-3

B.2 INSTALLING THE ID BARCODE READER (OPTION) ...................................... B-4

B.3 CONNECTIONG THE SAMPLER UFSU-1 (OPTION) ....................................... B-5

B.4 ADJUSTMENT OF THE SAMPLER UFSU-1 (OPTION) .................................... B-6

B.4.1 Adjustment Procedure ......................................................................... B-6
B.4.2 Preparation.......................................................................................... B-6
B.4.3 Adjustment Method.............................................................................. B-6

B.5 REPLACING THE SMPLER PROVE ASSY ...................................................... B-9

B.6 CONNECTING THE DP MIXING UNIT.............................................................. B-9

UF-50 S/M January 1999

 APPENDIX B INSTALLATION
B.1 INSTALLING THE FILTER ASSY NO.17 (C-2)

B.1.1. Pre-installation
Verity and record that there is no problem in the sheath pressure and UF-Check (Fscw) analyzed
data.
Fscw : Approx.80 ch
Sheath Pressure : Approx. 0.22 - 0.24 kg/cm2

B.1.2. Bubble Removal of the Filter (1)

1) Prepare approx. 20% solution of the ethanol (approx. 500mL). Use the distilled water for
solvent.
2) Dispense ethanol solution in clean container of steady shape like a beaker, etc.
3) Connect approx. 300 mm of Tube Polyurethane 3.4 mm x 1.8 mm with nipple positioned at
the lower part of the filter (IN).
4) Connect syringe (30 - 50 mL) to Tube Polyurethane connected to the upper part of the filter
(OUT) using Tube Silicone (5 mm x 2 mm).
5) Put the tube connected to the lower part of the filter (IN) in the ethanol solution in the
container.
6) Repeat aspiration and discharge using syringe to fill the ethanol solution in the filter.
7) Remove small bubbles existing inside the filter completely by pulling syringe strongly with the
edge of the tube inserted in solution. At this time, aim the syringe at under, and repeat
aspiration and discharge until small bubbles come off completely with the condition that
bubbles do not flow back into filter.
8) Make sure the bubble does not come in even if the ethanol solution is strongly aspirated by
syringe.
9) Discharge the ethanol solution in the filter.

Syringe

20%
ethanol

Fig B-1: Bubble Removal of the Filter (1)

UF-50 S/M B-1 January 1999

 B.1.3. Bubble Removal of the Filter (2)

1) Dispense 1L of CELLSHEATH to a clean container (beaker, etc.)

2) Put the tube connected to the lower part of the filter (IN) in the CELLSHEATH container of
step 1).
3) Repeat aspiration and discharge using syringe to fill the CELLSHEATH in the filter.
4) Remove small bubbles existing inside the filter completely by pulling syringe strongly with the
edge of the tube inserted in CELLSHEATH. At this time, aim the syringe at under, and repeat
aspiration and discharge until the small bubbles come off completely with the condition that
the bubbles do not flow back into the filter.
5) After the CELLSHEATH is filled in the filter and the bubbles were almost removed, block up
tube polyurethane (at A) to the upper part of the filter (OUT) by forceps.
6) Be sure tube polyurethane is block up at A.
7) Remove the syringe.
8) Remove tube polyurethane connected to the lower part of the filter (IN).

Syringe

CELLSHEATH

Fig B-2: Bubble Removal of the Filter (2)

UF-50 S/M B-2 January 1999

 B.1.4. Installation of the Filter

Install the filter filled with CELLSHEATH on Rear Panel of UF-50 Main Unit. At this time,
tubes A is blocked up by forceps.

1) Fix the filter on Rear Panel of UF-50 using screw binding M3x6.
2) Be sure the sheath line tubes (3.6 mm x 1.8 mm) stuck out of rear panel.
3) Block up tubes on both sides (B and C) by the forceps.
4) Remove the tube silicone connected these tubes.
5) Connect the end of tube polyurethane marked as “IN” to the nipple (IN) located at the lower
part of the filter. At this time, verify that the tube is not disconnected from the nipple even if
pulled strongly.
6) Disconnect tube polyurethane (3.6 mm x 1.8 mm) from the nipple “OUT” to the nipple (OUT)
located at the upper part of the filter.
7) Connect the end of the tube polyurethane marked as “OUT” to the nipple (OUT) located at
the upper part of the filter. At this time, verify that the tube is not disconnected from the
nipple even if pulled strongly.

Rear Panel
A Rear Panel C
C

C
C OUT

Silicone
Tube
IN

B B
B

Sheath Line Tubes

Fig B-3: Installation of Filter

B.1.5. Bubble Removal inside Filter

1) Remove the forceps both B and C.

2) Turn the power of UF-50 ON, and execute the continuous operation as follows to fill the
sheath reagent in the filter.
[Service] à [Setting] à [Continuous]
3) Make sure the bubbles outside the filter are removed and there is no bubbles in the tube.
4) Remove the bubbles existing in the filter as much as possible by knocking lightly by hand.
5) Display analysis result screen, and go on “continuous operation” until the following condition
is satisfied.

[Condition]
1. RBC, WBC, EC, CAST < 1.0 (count/uL)
2. BACT < 400 (count/uL)
3. Total < 300 (count/uL)

UF-50 S/M B-3 January 1999

 6) If total count remains high (over than 1000) even after continuous operation is executed 100
times or more, perform the bubble removal again according to Step: B.1.2. Bubble
Removal of the Filter (1). On the other hand, if total count decrease gradually, go on
“continuous operation” until the above condition is satisfied. (Normally approx. 150 - 200
times.)
7) Verify whether [Condition] described at step 5) above is satisfied regularly or not. Measure
UF-CHECK and make sure data is not changed between before and after the filter
installation. Especially, make sure Fscw data is not changed, and adjust if necessary.

B.2 INSTALLING THE ID BARCODE READER (OPTION)

1) Turn OFF the power switch on the Main Unit, if applicable.
2) Remove the right side cover.
3) Remove the front cover B.
4) Remove the decoration panel No. 18.

Decoration Panel No.18

Figure B-4: Removing the Decoration Panel No. 18

5) Disconnect the connector from the J2 connector of wiring cord No. 262A.
6) Install the ID Unit on the Main Unit chassis using two M3×6 binding screws provided.

Wiring Cord No.262A

J2

ID Unit
M3x6 Binding Screws

Figure B-5: Installing the ID Unit

7) Connect the ID Unit wiring cord to the J2 connector of the wiring cord No. 262A, and bind the
cords with a clamp.
8) Reinstall the right side cover and front cover B.
9) System Service representative will make the soft ware setting to recognize ID Reader.
10) After turning ON the power, perform following steps 11) and 12).
11) Press [Interfac] in the Service Setting submenu. Select “ 1: Connect” for Barcord setting.
Press [Return] then [Set] to make the setting effective.
12) Perform “Samp. Test” in Maintenance screen to confirm the ID reader reads correctly.

UF-50 S/M B-4 January 1999

 B.3 CONNECTING THE SAMPLER UFSU-1 (OPTIONS)

1) Pull out the front lower panel toward you.

2) Remove four M3×6 binding head screws of the ball catch using a screwdriver, then remove the
ball catch.

Ball catch

Figure B-6: Removing the Ball Catch

3) Fasten the Sampler Assy (UFSU-1) using four M3×6 binding head screws (provided).
Rack Arrival sensor (Switch Assy No.96) is supplied with UFSU-1 Kit and it should be
fastened using two M3×6 binding head screws (provided). 27

Tube Detection sensor

UF-50 sampler assembly

Rack Arrival Sensor (Micro SW) 18

Photointerrupter

Right Limit sensor

Left Limit sensor Cover Mounting Plate

Rack Position Binding head Binding head
sensor screws (M3X 6) screws (M3 x 6)

Sampler cover-C Sampler cover-B

Binding head screws (M3 x 6)

Figure B-7: Installing the Sampler Cover 23

UF-50 S/M B-5-1 Revised August 2005

18 TB200339 23 TB200409 27 TB200520
 4) Connect the wirings as shown below. 23

[STN4] :To MOTOR

[J56] :To TUBE DETECTION SENSOR
[J57] :To RACK POSITION SENSOR
[J58] :To RACK ARRIVAL SENSOR
[L1] :To LEFT LIMIT SENSOR
[L2] :To RIGHT LIMIT SENSOR

Figure B-7-2: Cable Connections 23

5) Fasten two Cover Mounting Plates using four M3×6 binding head screws (provided).

6) Fasten the Sampler Cover-B using two M3×6 binding head screws (provided).

7) Fasten the Sampler Cover-C using two M3×6 binding head screws (provided).

8) Install the Sampler Cover to the Main Unit.

9) Using two M3×6 binding head screws and two M3 toothed washers (provided), fasten the
Sampler Cover and two Cover Mounting Plates.

UF-50 S/M B-5-2 Revised February 2004

18 TB200339 23 TB200409
 10) Binds the cords by tie wrap not to be caught by the Sampler Cover A.

Binding head
screw
(M3 x 6)
Toothed washers
(M3)
Sampler

Figure B-8: Fastening the Sampler Cover A

B.4 ADJUSTMENT OF THE SAMPLER UFSU-1 (OPTIONS)

After installation of UFSU-1, the adjustment is required. The following shows the adjustment
method after the installation of the UFSU-1 KIT.

B.4.1. Adjustment Procedure

1) Position adjustment of UFSU-1 and Sample Aspiration Pipette of UF-50 Main Unit.
Adjustment of aspiration position.
2) Position adjustment of Rack Arrival Sensor.
If a rack is not correctly set, there is a possibility for Tube Detection Sensor to function
incorrectly, causing abnormal Sampler analysis. This sensor avoids this problem.
3) Storage of setting values.
4) Adjustment of Tube Detection Sensor.
5) Verification of adjustment result.

B.4.2. Preparation

1) Install UFSU-1 Kit on UF-50 Main Unit.

2) Turn the power of the Main Unit ON and enter Service mode.
3) Set a rack on Sampler Table. At this time, set a tube at the tube position 5.
Note) This tube should be the one specified in UF-50 Operator’s Manual.

B.4.3. Adjustment Method

(1) Position setting of UFSU-1 and Sample Aspiration Pipette of Main Unit.
Move Sampler Table and Sample Pipette to the aspiration position (tube position 5).
1) Position setting for Sampler Table
a) Move Sampler Table to the Sampler analysis initial position.
In the Service menu.
Press, [Utility] →[ M.Check] → [SV/Motor]
Set as: [Motor No. 4], [Pulses: 3000 pulse], then press [Return] and [OK] key.
Move Sampler Table to CCW Limit position by pressing [M.Drive].
At this time, verify that the indication on the right bottom of the screen changed from
“CCW Limit OFF” to “CCW Limit ON”.

UF-50 S/M B-6 January 1999

 b) Move Sampler Table to the sample aspiration position (tube position 5).
In the [SV/Motor] menu, change as: [DIRCTION 0: CW], [Pulses: 1260 pulse], and then,
press [Return], [OK] and [M.Drive] keys.

2) Sample Pipette Aspiration position setting

Move Sample Pipette from Manual analysis ready position to sampler analysis aspiration
position.
a) Move Sample Pipette to the upper dead point.
In the [SV/Motor] menu, set as: [Motor No. 3], [Pulses: 2000 pulse], then press [Return] and
[OK] keys.
In [SV/Motor] screen, move Sample Pipette to CW Limit position by pressing [M.Drive]
key.
At this time, verify that the indication on the right bottom of the screen changed from “CW
Limit OFF” to “CW Limit ON”.
b) Move Sample Pipette to the initial position of the F/B drive direction.
In the [SV/Motor] menu, enter as: [Motor No. 2], then press [Return] and [OK] keys.
Move Sample Pipette to CW Limit position by pressing [M.Drive] key. At this time, verify
that the indication on the right bottom of the screen changed from “CW Limit OFF” to “CW
Limit ON”.
c) Move Sample Pipette to the Sampler aspiration position.
In the [SV/Motor] menu, change as:[DIRCTION 1: CCW], [PULSE: 750 pulse], then press
[Return] and [OK] keys.
In the [SV/Motor] screen, move Sample Pipette to the Sampler aspiration position by
pressing [M.Drive] key.
Also, in the [SV/Motor] menu, enteras:[Motor No. 3], [PULSE: 250 pulse], then press
[Return] and [OK] key.
Press [M.Drive] several times so that Sample Pipette descends on the upper surface of the
sample tube. (Sample Pipette goes down approximately 20 mm by pressing [M.Drive] key
once.)

3) L/R position adjustment of UFSU-1

Adjust the Left/Right position of UFSU-1 with regarding Sample Pipette position as the basic
position. If the adjustment cannot be done well, loosen the front two screws securing the
Aspiration Unit. Adjust the Aspiration Unit position first, and then adjust the Rinse Cup
position.
a) Loosen four Screw Bindings fixing UFSU-1, and align the center of the tube and Sample
Pipette position visually.
b) In the [SV/Motor] menu, enter as: [Motor No. 2], [PULSE: 1 pulse],
and enter [DIRCTION 1: CCW] ß(i) when you want to move Sample Pipette to the
forward direction, or [DIRCTION 0: CW] ß (ii) when you want to move Sample Pipette to
the backward direction.
Then, press [Return] and [OK] key.
In the [SV/Motor] screen, move Sample Pipette to the center of the tube by pressing
[M.Drive] key.
NOTE) At this time, write down the number of the pulse you moved.
In the case of (i), record as plus (+) pulse, and the case of (ii), record as minus (-)
pulse.

4) Resetting of Sample Pipette F/B pulse number

a) In Service program, press [Settings] then [Param] keys to display [MTR2-PULS 750].
b) Add the number of pulses recorded in Step 3) above to the default value of 750 pulses.
Ex.) If the recorded pulse is “-3”, 750 + (-3) = 747 pulses
And enter the calculated number in [MTR2-PULS], then press [Return] and [SET] keys.

UF-50 S/M B-7 January 1999

 5) Height adjustment of Sample Pipette (if required)
a) Remove Front Cover B.
b) In the [SV/Motor] menu, enter as:[Motor No. 3], [DIRCTION 1: CCW], [PULSE: 2000
pulse], then press [Return] and [OK] keys.
At this time, verify that the indication on the right bottom of the screen changed from
“CCW Limit OFF” to “CCW Limit ON”.
c) Loosen Screw Binding fixing the pipette.
d) Fix the pipette at the position so that the distance between the bottom of the tube and the
pipette tip is approximately 5 mm.
e) In the [SV/Motor] screen, enter as: [DIRCTION 0: CW], [PULSE: 2000 pulse], then press
[Return] and [OK] keys.
f) Reinstall the cover.

(2) Position Adjustment of Rack Arrival Sensor

a) Move Sampler Table to the Sampler analysis initial position.
In the [SV/Motor] menu, enter as: [Motor No. 4], [DIRCTION 1: CCW], [Pulses: 3000
pulaw], then press [Return] and [OK] keys.
In the [SV/Motor] screen, move Sampler Table to the CCW Limit position by pressing
[M.Drive].
At this time, verify that the indication on the right bottom of the screen changed from
“CCW Limit OFF” to “CCW Limit ON”.
b) Adjust the position of Micro Switch so that Micro Switch is turned ON when a rack is
correctly set. The sensor status is displayed at “TUBE” at left bottom of the [Machine
Check] screen

(3) Save the setting values

In order to store the setting values into the memory, turn the power of Main Unit OFF and ON.

(4) Adjustment of Tube Detection Sensor

1) Position setting of Sampler Table of UFSU-1.
a) Move Sampler Table to the sampler analysis initial position.
In the Service menu, press [Utility], [M.Check] and [SV/Motor] keys, enter as:[Motor No.
4], [Pulses 3000], then press [Return] and [OK] keys.
Move Sampler Table to CCW Limit position by pressing [M.Drive] key. At this time, verify
that the indication on the right bottom of the screen changed from “CCW Limit OFF” to
“CCW Limit ON”.
b) Move Sampler Table to the sample aspiration position (tube position 1).
In the [SV/Motor] menu, change as: [DIRCTION 0: CW], [Pulses: 30 pulse], then press
[Return], [OK] and [M.Drive] keys.

2) Adjustment of PCB No. 3048 (Sensor Board)

a) Adjust VR1 on the above sensor board as follows with setting nothing on the Sampler
Table.
D5 ON → D5 OFF → D4 OFF → D4 ON
Stop VR immediately after D4 turns ON.
b) Set the rack in which a tube is set at the tube position 1 on the Sampler and verify that D5
turns ON.
(5) Perform sampler analysis and verify the adjustment is correctly done.

UF-50 S/M B-8 January 1999

 B.5 Replacing the Sampler Probe Assembly (Optional Sampler)

1) Remove the probe support and sample probe from the sample arm.
2) Fasten the sample probe assembly (optional sampler) to the arm using two M3×6 binding head
screws (provided).

Sample probe assembly

Sample arm

Binding head
screw
(M3 x 6)

Figure B-9: Replacing the Sample Probe Assembly

B.6 Connecting the DP Mixing Unit (UF-50)

1) Fasten the mixing unit to the upper part of reagent case using two M3×6 binding head screws
(provided).
2) Connect three solenoid valve cords to the solenoid valve.
3) Connect one hydraulic connector from the hydraulic unit to the one by sandwitching the rubber
packing.
4) Connect one connector from the control unit.
5) Connect the upper part of the sample pipette using two tubes.
6) Fasten the clamp at the upper part of the reagent case.

2) Connect 3 Solenoid valve cords

4) Connect Control connector

1) Fasten the mixing unit

3) Connect hydraulic connector

6) Fasten the clamp

Binding head screw M3x6

5) Connect tubing to the Sample Pipette

Figure B-10: Connecting the DP Mixing Unit-1

UF-50 S/M B-9 January 1999

 27
7) Clamp the bundle of mixing line tubing.
8) Connect the mixing line tubing to the pipette.
9) Bind aspiration line and mixing line tubings temporary using tie wrap.
10） Connect the sensor connector to the connector from PCB No.3048.
11) Clamp the cord of PCB No.3048 using the clamp on the control unit.
12) Clamp tubings to the chassis using tie warps at two points.
13) Secure and cut tie wraps at three points.
14) Move the sample aspiration unit up, down, forward and backward to check it moves smoothly.

(12) Clamp tubings (9) Bind tubings

LCD Unit

(10) Connect the connectors

(7) Clamp
the tubing

(8) Connect the tubing to the pipette. (11) Clamp the cord

Figure B-11 Connecting the DP Mixing Unit-2 27

UF-50 S/M B-10 Revised August 2005

27 TB200520