SECTION 5 HYDRAULICS Table of Contents Page 5.1. Hydraulic Diagram and Sample Flow .. 54 5.11 Hydraulic Diagram.. 51 5.1.2 Sample Flow 54 5.2 Arrangement and Tubing of Valve Unit. 53 6.2.1 Arrangement of Valve Unit. 53 8.2.2 Tubing of Valve Unit. 53 5.3. Arrangement and Tubing of Chamber Unit 55 5.4 Arrangement and Tubing of Chamber Unit 56 5.4.4 Arrangement of Chamber Unit 56 5.4.2 Tubing of Chamber Unit 57 5.5 Explanation of Hydraulic and Mechani 8 5.5.1. Detector Block. nnn 5B 5.52 Reaction Unit. 514 5.5.3 Sample Aspiration Unit Assembi 5-18 5.5.4 Volumetric Syringe : 5-23 5.5.5. Syringe Unite : 15-24 5.5.6 Reagent Aspiration Uni... 5-26 5.57 Incubation Unit No. 12.. 5:27 UF-100 SM qa &Section 5 Hydraulic and Mechanical Systems 5.1 Hydraulic Diagram and Sample Flow 5.1.1. Hydraulic Diagram Tneubation ow | eee | Unite. 12 Coll mm upto ase | a et % a ae |Charging| Urine Sample = ee [a ine Sanpe a © ® Volumetric Syringe Unit ‘Sheath Syringe Figure 5-1 UF-100 Hydraulic Diagram 5.1.2 Sample Flow (1) Undiluted urine sample of approx. 800 uiL is aspirated from pipette through sampling valve (SRV) by the action of volumetric syringe. (2) SAV rotates and aliquots 400 nL of undiluted urine sample. (3) Undiluted urine sample of 400 iL is dispensed to the reaction unit with 1160 pL of diluent by the action of volumetric syringe. ‘= Reference > {560 nl of URINOPACK is consumed per sample, but actually 1160 pis used for alaion. Volumetric syringe ‘will aspirate and dispense 1560 yL of URINOPACK. The diluent syringe tubing is connected to the Reaction ‘Chamber through the SRV. When the diluent is dispensed to the Reaction Chamber, SRV rotates, 400 uL of 'URINOPACK is replaced by 400 pL of urine, and 400 ul of urine and 1160 uL of URINOPACK is dispensed {together into the Reaction Chamber. The 400 ul of URINOPACK remained in the SRV is used as the rinse solution for the pipette, (4) At the same time, 40 uL of dye is dispensed to the reaction unit to produce 1:4 diluted sample. (6) The ciluted sample is mixed with propeller for approx. 10 seconde and is heated at a predetermined temperature to perform dye reaction. (6) Sample in the reaction unit is aspirated to the charging line by vacuum in the Waste Chamber-2 and the predetermined amount is pushed out to the jet nozzle by the sheath syringe. UF-100 SM Bt MAY 1996(7) Atthe same time, sheath fluid heated to a certain temperature is pushed into the flow cell to form sheath fiow. (8) Sample passes through the center of flow call surrounded by sheath fluid and measured by Detector Block. ‘Sample of 36 yl (undiluted urine of 9 uL) is measured in 23 seconds. = Reference > '36 iL of sample is measured in 23 seconds. This sample contains 9 wL of undiluted urine, but the measurement time with the normal sensitivity is 20 seconds, as shown below. Therefore, the sample volume used for normal sensitivity is 7.83 ul per sample. } High Bact ‘Measurement initialize ‘Normal Measurement Figure 5-2 Measurement Time ‘= Reference > | Sheath fluid, diluent and reaction sample are heated to 35°C due to the following reasons. (1) A constant temperature assures to stabilize the speciic gravity, pH and dyeing characteristics. (2) Amorphous urate salt melts at 35°C. If amorphous urate, which is frequently seen in the acid urine, exists in the sample, this interferes urine cell analysis. UF-100 Sm 52 MAY 19965.2 Arrangement and Tubing of Valve Unit 5.2.1 Arrangement of Valve Unit ‘Valve Unit (1) Figure 5-3 Arrangement of Valve Unit 5.2.2 Tubing of Valve Unit (1) Valve Unit (1) ‘Sheath Incubation Unit (Bubble Removal [-———— Rinse Chamber.2 Waste Chambor.2 @ —— wer | 444 -== Waste Chambor2®—] cose 05 kolema LF [eee | —— Fel ala] Olah, erate Cubitainer oti | Bt | eetiatne Jet Nozzle Holder @ Rinse ‘Sheath (Sheath Pressure) ‘Volumetric Volumetric noe eae Chember Syringe Syringe veo (@iuent) ye) shea MAP 4 Rinse Chamber-2 Chamber " posomtnna [ale ‘Manual Valve Dye aia ortho stbah 2.2 kgloma ee Incubation Unit Syringe |__Pinse Cup wnbor ‘Sheath Syringe “(Uiney — Rinse Cup Figure 5-4 Tubing of Valve Unit (1) ur-t009m 53 MAY 1996,@) Valve Unit (2) ing Chamber cugse Me? — CvindorPipete Back Foreard) | | 10s |exaee Waste Chamber-2® a] 8lalalalalaly ale eae eal e |e T T vss | Mvsa | wvet (eye tee eae) ylinder (sR) ting crater cyte (Pipote BackeFoward) 400 mmHg 05 kglom2 cuss | se of of ‘oF 4X8 0 liar] az 3 38] 8] 49 |lat lay 22kglom2 = Mva7 | vss] wvss| eer “Trap Chamber (WC-2) ) | (P) \—Trap Chamber (wos) | waste chamber © Figure 55 Tubing of Valve Unit (2) (3) DP Unit sc0mmitg os kgteme Fleaction Chamber (preheat) [I Rinse chamber2 Tho) i es ees | ER, 5 | as] 47 te Figure 5-8 Tubing of DP Unit [= Reference > Koganel solenoid valves are used for UF-100 as well as for SF-3000 and CA-6000. UF-100 SM 54 MAY 1996,5.3 Solenold Valve Function ‘SV No. [MV No. | Function when ON i 7 ‘Sample Charging Line (OUT) Open. | 2 57 Dye Dispense to Reaction Chamber Ad 1 3 3 Fiow Cell Air Bubble Remove (Flow cell air removal sequence) | A6 i 4 4 Priming Flow Cell Detergent (Fiow cell air removal sequence) | AG. i {s 5 Flow Cell Exit Open D6 | é 6 Flow Gell Ext Switching (WC1WG2) E6. (7 ai ‘Sheath Line Air Remove (trom Incubation Unit No. 12) DS 8 \s | Rinse Chamber 2 Prime (400 mmHg side) Da 3 [Rinse Chamber 2 Prime (Sheath fluid side) o 10 | Rinse Chamber 1 Prime (400 mmHg side) 87 i Rinse Chamber t Prime (Sheath fluid side) 10 12. ‘Sheath Chamber Prime (400 mmHg side) 37 13: ‘Sheath Chamber Prime (Sheath fluid side) rn 14 Diluent Dispense to Reaction Chamber Ca 12 15. Dilution Syringe Prime C2 43 46 Dye Syringe Prime. cz 14 ‘ot used) 4s Er ‘Sheath Chamber Open O7 Ge —h9 Flow Cell Nozzle Rinse D7 (7, (20. ‘Sample Charging Line Rinse Dé 18 48 Reagent Asp. Unit (Diluent) Prime (Air Remove) Dé (9 49 Reagent Asp, Unit (Dye) Prime (Air Remove) D4 [ao 23. ‘Sample Aspiration Line Rinse. Da fat 24 Rinse Cup Rinse [03 22 [25 Mixing Chamber Rinse C2 (2a [26 Pipette Droplets Sweep D2. 24 |'58(P) | Reaction Chamber Sample Drain C4 i 25 60. Charging Line Bypass Ga | 26. '58(P) | Sample Charging IN cs 27 30 SRV Rotate Aa, 28 Gl Rinse Cup Drain DI I 29 ‘32 Pipette Air Blow B2 30 33, Pipette Forward/Backward At 31 34, Mixing Chamber Open to Air ct 32, [35 Filter Drain, DP Air Blow Fa [3s 136 WC2 Drain Pressure Apply Es 34 [37, 54 | WC2 Drain FB EA 137, 53__| WCt Drain. Fe (36. 41, 55 _| WC3 Drain F5 [37 = DPI Prime: AZ 38. = ‘DP2 Prime, Aa, 38 “45, 46 | Reaction Chamber Rinse C5 | 40. a7. ‘OP Rinse Fa. Manual Valve | 56 Diluent Manual Aspiration Aa UF-100 SM 55 MAY 19965.4 Arrangement and Tubing of Chamber Unit 5.4.1. Arrangement of Chamber Unit Figure 5-7 Arrangement of Chamber Unit UF-100 SM 56 MAY 19965.4.2 Tubing of Chamber Unit (1) Waste Chamber 1 OPEN MV6 ——— MV5 ——— Isolation | ‘Chamber 0.5 kg/em2- Mv7 —— Sheath incubation Unit (Bubble Removal) Figure 5-8 Tubing of Waste Chamber 1 (2) Waste Chamber 2 lsdlation Chamber-—~ mvs. ——~ Mv Jet Nozzle Holder ——— V1 Myst —— Mixing Chamber M56 (Manual Valvoy ‘Mv60—— MV58-——— Reaction Chamber [— Mvse —— var —— OPEN = 0.5 kg/em2, 400 mg Figure 5-9 Tubing of Waste Chamber 2 (3) Waste Chamber 3 [—— Mvas — Diuent Chamber Dye Chamber — vig — Ann eS Finge Cup— aves. vet — 400 mmtig es tiene Figure 5-10 Tubing of Waste Chamber 3 F-10081 87 MAY 19985.5 Explanation of Hydraulic and Mechanical System 5.5.1 Detector Block 5.5.1.1 Outline ‘Signal Light System, ght Detector System (eee i il nee D Beam Spot Focusing System z @shoathFow System Tight Detector System © Elec Resistance f Detection System Mi: Dellection Holder No.3 Assy AP: Pinhole Mz: Detection Holder No.2 Assy DME Diehl Miror midenser Lens PD: Photo Diode 12: Object Lens F:Sharp CutFiter_S: Boam Stopper PMT: Photomutiplier Tube L: Collector Lens Figure §-11 Optical Configuration of UF-100 (1) Beam Spot Focusing Parallel beam from Argon taser (wave length: 488 nm) is reflected by two dichroic mirrors where unnecessary light (electric discharge ight) is removed, and is focused by condenser lens to form a beam spot. Focal length of the ‘condenser lens is different in vertical section and horizontal section; beam in horizontal section is focused at the ‘beam stopper, and beam in vertical section is focused atthe flow cell. (2) Sheath Flow ‘Steady sheath flow is obtained by keeping temperature and pressure of sheath fiuid at a constant value (35°C, (0.10.2 kg/em). As a result, particle inthe flow cel Keeps a steady state. (Long and slender parte such as cast flows vertically in the flow cell.) The sheath pressure of 0.1 - 0.2 kg/cm® is determined by the Fscw value of the UF CHECK. (9) Collection of Forward Signal Light Laser beam passing through flow cell is shielded by beam stopper to remove the direct beam, and collected by object lens. (4) Forward Scattered Light ‘The collected signal light passes through pinhole to remove unnecessary light (electric discharge light). This signal light is divided into scattered light and fluorescance by the third dichroic mirror. ‘The scattered light reflects on the dichroic mirror, and itis collected by collector lens and is led to photo diode. (6) Forward Fluorescence Fluorescence with longer wave length than scattered light passes the dichroic mirror, and it is removed of unnecessary light by a fitter and is fed to Photomuttiplier. (6) Electric Resistance Detection Electric resistance when particle passes is measured by a palr of electrodes installed in the flow cell, UF-100 8 58 MAY 19986.5.1.2 Specifications and Functions (1) Beam Spot Focusing {(@) Argon laser source - Type: + Output: = Wave Length: + Focal length in vertical section: + Focal length in horizontal section: = Short diameter: Long diameter: (b) Condenser Lens (©) Beam Spot Light Intensity GLG3070A (NEC) < For Japan Domestic only > Model 161C (Spectra-Physies) < For export only > 7.5 mW (Oscilating) 3.5 mW (Stand-by) 488 nm 8.93 mm (distance to flow cell) 9.26 mm (distance to beam stopper) 85 um 285 um + Light Intensity from the laser ditibutes in 4 normal Gaussian cuve + Light intensty decreases as X increases (separated epar from the center o the beam). Tight Intensity Figure 5-12 Light Intensity Distribution of Beam Spot (2) Sheath Flow (a) Sheath Flow Formation _- Sheath pressure: = Width of sample flow: = Velocity: (©) Flow cell (0) Jet nozzie 04-02 kg/om? Approx. 20 um Sis ‘Quartz glass 0.25 mm square 4mm square Ceramic 02mm 13mm Flow (Front View) Flow Figure 5-19 Laser Beam and Sample Flow in the Flow Cell ‘UF-100 SM MAY 1996/ (1) UF-100 adopts the electric resistance method besides the optical detection method. its flow cell is made partially narrow 2s shown and when particle passes this narrow passage the electric signal is obtained. (R-3000) (UF-100) (2) When installing the flow cell, face the surface with white marking toward the direction of incident laser beam. ‘White markings indicating light input direction | Figure 5-14 Flow Cell Comparison and Direction Markings (8) Collection of Forward Signal Light (@) Beam stopper width: 0.6 mm (4) Forward Scattered Light {@) Sensor = Photo diode (0) Preampifier = PCB No. 2127 (6) Forward Fluorescence (@)Fiter + Optical Filter No. 2 (Dichroto miror) (b) Wave length selection fiter = Optical iter No. 8 (©) Sensor = Photomuttiptier (@ Preamplifier = PCB No. 2128 (6) Electric Resistance Detection (@) Preampltier = PCB No, 2129 Isolation Chamber ‘To POB No, 2129 Connector ‘ToWaste Chamber-2 Flow Cell Holder From Reaction Unit with Electrode Figure 6-15 Outline of Electric Resistance Detection F-10051 540 MAY 1998[ |Flow Cell Holder also functions as an electrode, and this is why this part is made of stainless steel (SUS). «< Reference > The comparison of optical specifications with R-2 Series is shown in the following list. f UF-100 ea Series, [Argon taser ‘GLGSO7OA (NEC) | Model 1616 (Gpectra-Physios) | Model 161C (Spectra-Physics) ‘Output 7.5 mW (Oscllating), 3.5 mw (Stand-by) Beam spot (short diameter) ‘Beam spot (long diameter) 76 um Sheath pressure 0.2 kgiem®. 0.28 kalom® ‘Sample flow width of in the flow cell 0 um ‘Approx. 10 mm Weloey ete fw cll Sms inside dimension of fow cal [025 mm square 0.3 mm square ‘Outside dimension of fow cell | ‘4mm square Inside diameter of jet nozzle | 0.2mm, Outside diameter of jet nozzie | 4.3mm 1 Forward scattered light pre-ampitier | PCB No. 2127 __| PGB No. 2075 i Fluorescence pre-amplifier ‘PCB No. 2126 PCB No, 2074 Fitter 1 Dichroic mirror (Optical Filter No.2) (Wave fength selection fiter f ‘Optical Fiter No. 8 5.5.1.8 Arrangement of Each Parts (1) Detector Block (Sumbot [Description ‘Symbol _| Description it Deflection Holder No. 3 Assy 5 ical Unit No. 4 Ass 2 Optical Source No.3 Assy 6 Incubation Unit No. 12 Assy i Photo Detector No. 10 Assy 17 ‘Glass Chamber GC-27 Assy (a PCB No. 2128 te Deflection Holder No.2 Assy} ‘Note: Part marked with (*)is common with R-2 Series. Figure 5-16 Detector Block UF-100 UF-100 SM eat MAY 1996(2) Optical Unit No. 4 Assembly i Symbol _| Description. ‘Symbol _| Description 1 [Condenser Lens UF-100 3° Beam Stopper No. 3 Assy iF | Fiow Cell Unit No. 7 ‘Note: Part marked with (*) is common with R-2 Series. Figure 5-17 Optical Unit No. 4 Assembly. a Electrode (+) © Beco +) Eactose tbat) Figure 5-18 Details of Flow Cell Unit No. 7 U-100 SIM 52 MAY 1996(8) Photo Detector No. 10 Assembly (Dotails of Pre-amp Unit No. 8 Assy) {Symbol [Descristion Symbol_[ Description 1" Photo Detector Holder No. 2 Assy 14" ‘Optical Fiter No. 8 2 Pre-amp Unit No. 9 Assy Ga ‘Optical Filter No. 2 Assy a Photomultipier Tube R928 6 PCB No. 2127 ‘Note: Part marked with (*) is common with R-2 Series. Figure 5-19 Photo Detector No. 10 Assembly Reference > 1 In the parts consisting the Photo Detector No. 10 Assembly, PCB No. 2127 (@ ) in the Pre-amp Unit No. 9 | Assembly ( @ ) is the only one part that is not compatible with Fi-2 Series. | F-10051 513 MAY 19985.5.2 Reaction Unit 5.5.2.1 Outline This unit dyes the diluted sample, The following processes ate also performed by this unit. + Sampling ofthe undiluted urine samp {haning and heating of the cuted sample + Preheating of diluent and detergent + Measurement of electric conductivity Sticone 1¥6(15) 2 To Sheath Syringe Dye Manual Asp. Valve 56 Silicone) 6(15) ® | To Volumetric Syringe (Dye) 7 Reaction Unit [Conductivity Sensor ‘SRY drive oyinder sav iron Dituent Sheath (Rinse) Slicone 135418). ra / To Volumetic To PNET 25 Em singe (Dhuen) Figure 5-20 Tubing Diagram of Reaction Unit 5.5.22 Specifications and Functions (1) Sampling Rotor Valve (SRV) (@) Dispensing Volume of the Sample: 400 iL SRV Rotation Rotor Valve From Volumetic Sytinge (Divert) ToP pete <—— Aghot urine sample by this tubing Fined Valve (ube Teton 1 mmiD x2 mmOD, 485 mm ong) Figure 6-21 Aliquot of Sample by SRV UF-100 8M Bt MAY 1996,‘ = SRV of UF-100 is composed of two discs unlike three discs of previous models. ~ Teflon tube 1 mmiD x 2 mmOD is used for aliquot. Higher accuracy is required for this tubing than the ordinary hydraulic tubings. (Inside diameter tolerance 1,0 + 0.03)- 0.02 mm) (b) Method of Sample Aspit = Electric conductivity Method Electrodes are attached to the tubings connected to SRV. The presence of the sample is detected by the ‘conductivity change. tion Sensor tamm 12mm ‘Sampling Valve eI Pc No. 3048 (Driver Board) ‘Sample Aspiration Sensor Figure 5-22 Outline of Sample Aspiration Sensor (©) Range of Detection: Detectable for the sample with conductivity of § - 80 mS/cm. [= Reference > = Unlike blood, urine sample varies in the wide range from almost colorless to dark color, Therefore, photodiode [cannot detect the presence of the sample as in the case of blood counters. ‘Conductivity of water is onl ‘2:mS/em and error is issued when water is aspirated. (2) Reaction Chamber {@) Mixing function: ‘Mixing motor mixes at 1500 + 200 rpm. (VFS on PCB No. 3048) ‘This speed is monitored with the Hall Sensor. (b) Heater function The dyed sample, the diiuent and the rinse fluid are heated at 954 2°C. (Room temperature: 15 - 30°C) (6) Protection function: ‘Thermal fuse of 76°C. (8) Conductivity Sensor (Incubation Unit No. 13 Assy) Because of fow dilution ratio (four times), electric resistance detection signal depends greatly on sample conductivity. Therefore, conductivity of the diluted sample is measured in each measurement to correct the detection signal. (a) Measurement sensitivity: Detectable for the sample with conductivity of 5 - 80 mS/cm. (0) Heating functior The dyed sample is heated at 35 + 2°C. (Room temperature: 15 - 30°C) (6) Protection function: ‘Thermal fuse of 76°C. Heater Thepmal Fuse» Thermistor ‘To POB No, 3048, (Bensor Board) |<< From Reaction Chamber ToFIOW Cat Conducvty Sensor ToPCBNo.2120 Not Asser stcene Tbe ST (Pre-empiior Boars) Figure 5-23 Structure of Conductivity Sensor (Incubation Unit No. 13 Assy) UF-100 5M 545 MAY 1986.Holding Material No, 131 Figure 5-24 Structure of Conductivity Sensor No. 4 Assembly PCB No, 3048 (Sensor Board) PCB No. 2129 (Pre-ampitier Board) Figure §-25 /O Specification with Other Units 55.23 Arrangement of Each Parts (1) Reaction Unit UF-100 Assy ® @ =O} 3 Symbol_| Description Symbol | Description ] 1 ‘Valve Unit No. 118 a Reaction UnitNo. 5 2 ‘Air Cylinder 25 mm Dia ST-20_[5. ‘Sample Aspiration Sensor No. 1 Assy _| 3 Valve Unit No. 117 6 incubation Unit No. 13. Figute 5-26 Reaction Unit UF-100 Assembly UF-100 9M 18 MAY 1996,(2) Reaction Unit No. 5 jymbol_| Description: ‘Symbol_| Deserition 1 ‘Motor Block No. 41 Assy [11° [Heater Unt No. 6 Assy a Hall Sensor No. 4 Assy 12* | Fuse Assy No. 1 cu Rubber Packing No. 54 13° | lubber Packing No. 33-8 a ‘Shaft No. 55 14° | Rubber Packing No. 33-A é ‘SRV Fixed Valve No. 26 Assy 115° | Thermal Block No. 14 6 ‘Switching Valve No. 4 ‘16 | Rubber Packing No. 41 Z Washer No. 3 17° | Mixer Paddle No. 5 8 ‘SRV Fixed Valve No. 27 18. Pipette Support No. 24 Ea ‘Sample Rotor Fixture No. 4 Assy 19 “Thermistor No. 35 Assy | 10° | Key No. 6 1 Note: Part marked with (")is common with F-2 Series. Figure 5-27 Reaction Unit No. 5 Assembly [= Reference > ‘Temperature of the fluid which flows into the unit depends on the room temperature. Thermistor No. 35 is ‘consisted of two thermistors; one for controling and the other for compensating. The thermistor for controlling is| built-in the inside of the unit to control the temperature of the unit to 85°C. The thermistor for compensating is, installed outside the unit and monitors the room temperature to compensate the sheath fluid temperature. (Adjustable for the room temperature of 15 - 30°C). UF-1008”M 57 MAY 19965.5.3 Sample Aspiration Unit Assy 5.5.3.1 Outline (1) The presence of the sample is detected by the electric conductivity through the electrodes. (2) A thin pipette and a thick pipette is combined together. The thick pipette mixes the sample by aspirating and dispensing. The thin pipette aspirates the predetermined amount of sample. {@) Outside and inside ofthe pipette is rinsed after the sample is aspirated. 400 mmHg 08 kglom2 sor fF wer ftLe T Waste Chamber-2 Figure 6-28 Tubing Diagram of Sample Aspiration Unit 5.5.3.2 Specifications and Functions (1) Detection of Sample Presence ‘The presence of the sample is detected by the electric conductivity through the electrodes provided in the mixing chamber above the pipette {a)Method of Detection: Electric conductivity {b) Range of Detection: Detectable forthe sample with conductivity of § - 80 mSYem. —[T] Te PoB No, 3048 (Sensor Board) tment Electric conduction | Repeat five times of aspirating and at the first or the second aspiration __| dispensing, and then aspirate the sample. 'No electric conduction ‘Drain the aspirated sample, and move to at the first and the second aspiration_| the next sample. Figure 5-29 Detection of Sample Presence UF-100m 548 MAY 1998(2) Pipet Crash Detection ‘The pipette crash is detected by a microswitch, And the pipette is protected. (2) During horizontal motion o pipette ‘When pipetie moves by any causes during is withdrawal motion and microswitch 81 is tumed ON (Closed —> Open), "Pipetie Crash 1 Error is issued. Pipette withdrawal motion is suspended immediately and pipette retums to the inital position (front position). When the wiring to the microswitch St is cut, the same error occurs. (b) During vertical motion of pipette (During sample aspiration) When pipette moves and microswitch S2 is tuned OFF (Closed -> Open), “Pipette Crash 2 Error” is issued. Pipette lowering motion is suspended immediately and pipette retums to the intial position (upper position). 2 a} ae q Z q SS cera ee [Error Message | State of SW | Monitor Timing Pipet Crash 1_| SWi-ON | During back and forth motion of pipette | Pipet Crash 2| SW2-OFF | ‘During up and down motion of pipette from and to the sample tube: L {Not monitored during up and down motion from and to the Rinse Cup) Figure 5-30 Pipette Crash and Error Message (@) Sample Mixing ‘Sample is mixed by the vertical motion of the pipette and repeated aspirating and dispensing ofthe thicker pipette by switching the pressure and the vacuum of diaphragm pump (@) No. of aspirating and dispensing: Five times (©) Pipette stroke: ‘Approx. 10 mm (6) Displacement: 2.0 mL (Diaphragm pumps of 1.2 mL and 0.8 mL drive at the same time.) {@) Inside diameter of pipette: 41.99 mm diameter (pipette for mixing). 200, sv 10 |p peaoy Meh 100 uo 37_[DPI ASPIRATE. '36_[0P2 ASPIRATE. 5 Be 5 Figure 5-31 Sample Mixing Sequence = Reference > DP1 and DP2 used for aspirating the sample is not driven simultaneously, but driven slightly delayed. This is to ]weaken the aspiration speed of the sample, and to prevent from back-flowing the sample, UF-100 SM 519 MAY 1996Aspiraing Dispensing =—_— Repeating Figure 5-2 Mixing of Sample (8) Aspiration of Sample £800 pL of the sample is aspirated with the thin pipette. The volume of aspiration is same for each of manual or sampler mode. (@) Sample aspiration ~ Amount of necessary sample: 1.0 mL oF more (manual) and 4.0 mL. or more (sampler). = Amount of sample aspiration: me = Specific gravity of aspiratable sample: 10 - 1.050 g/cm? + Inside diameter of pipette: 7 mm diameter (pipette for aspiration). r{_— Meira ChanberNo. 6 (BE rae Detalts of Pipette Electrode (-) 4 _— l Aspiration Pipete Mixer Pipette (07mm ameter) (1.88.mm dameter) Figure 5-33 Details of Pipette and Mixing Chamber Pipette Complete No. 4 (6) Filtration of Sample Dir and dustin the samp are removed by the provided 250 um mesh fitr located between the pipette and he RV. = Reference > Mesh Fitter is a maintenance-free part and no periodical replacement is required. The reasons are explained as follows: (1) The rinse fluid washes the filter with a back-flow in every cycle of operation. (2) The detergent CELLCLEAN steriizes and cleans the fiter in the shutdown sequence. UF-100 SM 520 MAY 1996Micro-switch (roman) woMao | eee saciroreare, | Pope. 350 |g “Coney | Diver poe Sw ico stch SvisM 8 Sy « joard) system: ei ) FOB No. 3048 Sample Asp. Sensor_| (Sensor Board) be | apes de ed ee A or ‘Sample Flow =| ‘Sample Aspiration Figure 6-34 VO Specification with Other Units 5.5.33 Arrangement of Each Parts (1) Sample Aspiration Unit Assy L ° [<3 ‘Symbol | Description [Symbo!_| Description | 1 Pipette Unit No. 6 Ws Rinse Cup No. 48 Ass 2 ‘Tube Teflon 1.2 mmiDx20mmO0 6 Photo-Interrupter GP-1A0SHA. 3 UF Sample Filer 7 Non-Retum Valve No. 2 a 4 Pipette Comp. No. 4 4 Figure 5-35 Sample Aspiration Unit Assembly = Reference > (1), The top or the bottom stop position of the pipette can be adjusted by sliding the upper or the lower photo- interrupter position, as shown with © in Figure §-35. |) The front stop position of the pipette can be adjusted by sliding the stopper-bolt, as shown with @ in Figure 5-37 in the next page. (8) The to ght poston othe Sample Aspiration Unt canbe aduste by losening tho fing Serows othe assis. UF-100 SM 521 MAY 1998S é alo oje rloo Ton ot Mo Irate (Nipple Postion of Mixing Chamber) Ga Mechanical Valves for Pipette Crash protection Air Cylinder for diving Silicone 7*xSI20) 8 seo (60 a (F101 x pipette back and forth Figure §-38 Sample Aspiration Unit Tubing Diagram [= Reference > [When the pipette ascends, the mechanical valves tum ON and the pipette could move back and forth. (2) Pipette Unit No. 6 d ® ® [Symbol | Desoription. 2 mbol_] Description | fi ‘Asp. Check Sensor No. 1 5 Switch No. 90 Assy i} 2 Mixing Chamber No. 6 Assy] ‘ir Gyinder POA1OX76A_—__| 3 ‘Stopper Bolt USS4-20 7 Switch No. 91 Assy _ | 4 ‘Arm No. 58 UF-100 5M 522 Figure 6-37 Sample Aspiration Unit Assembly MAY 19965.5.4 Volumetric Syringe 55.4.1 Outline. Volumetric eyringe aspirates and dispenses the undiluted urine sample, the diluent and the dye. Reaction Chamber (preheat) PCB No. 3050 i it ema agent Aspiration Unit (Diluent) Reaction Chamber Reagent Aspiration Unit (Dye) sav Rinse Chamber-2 Figure 5-38 Outline of Volumetric Syringe 5.5.4.2 Specifications and Functions (1) Amount of Aspiration (@) Undiluted urine sample: 800 iL (Outer diameter of piston: 11 mm diameter) {) Dituent: 1560 iL (Outer diameter of piston: 11 mm diameter) (6) Dye: 40 uL (Outer diameter of piston: 3 mm diameter) 5.5.4.3 Arrangement of Each Parts "| @@ dE GO 3h o~Ag Symbol | Description ymbo!_| Description. 2 Piston Assy No. 26 6" Seal Tefion AR 10103-P11-AU 3 Piston Assy No. 27 F io Seal Teflon 480148246. 4 Switch No. 55 Assy Note: Part marked with (?)is common with R-2 Series, Figure 5-39 Volumetric Syringe Assembly UF-100 SM 523 MAY 19965.5.5 Syringe Unit 55.5.1 Outline ‘Syringe unit dispenses dyed sample into the flow cell. Fow Cell Reaction Chamber ‘Sheath Chamber \_____ finse Chamber-t Syringe Complete ‘Assembly No. § Figure 5-40 Outline of Syringe Unit 5.5.5.2 Specifications and Functions (1) Driving Speed (@) Low speed drive (Measuring): 360 pps (0) Medium speed drive (Aspirating Predrivey: 1200 pps. {c) High speed crive (Resetting): 3000 pps {2) Total Dispense Volume: 60 pil or more (18 iL (aspirating predrive) + 36 yL (measuring drive) + some). (8) Process Speed: ‘Approx. 36 iL of sample is analyzed in 23 seconds. (@) Vibration Reduction: A dumper is attached to the motor axis to reduce the vibration in low speed driving. UF-100 SM 524 MAY 19986.8.8.3 Arrangement of Each Parts ff AT elt ‘Symbol | Description [Symbol_[ Description tt ‘Syringe Comp. Assy No. 57 Fixing Material No. 27-8 2 ‘Dumper No. 1 ie ‘Sponge No. 19 a ‘Switch No. 55 Assy. * ‘Syringe Piston No. 2 a Motor PX245-03A-C10 10° | Seal No.8 | i Cylinder No. 33 417 | O-ring No. 12 (5 Fixing Material No. 27-A 4 Note: Part marked with (") is common with R-2 Series. Figure §-41 Syringe Unit Assembly r= Reference > [The only one diference trom the R-2 Series Syringe Units the Oumper No.1 (shown as @ in Figure 6-41). This | dumper will reduce the high-frequency vibration sound while sample is pushed out to the flow cell. R-2 Series will not need this dumper because the rotation speed is different. Rolaton speed during analyzing In UF-100: 360 pps Rotation speed during analyzing in R-2 Series: 90 pps. UF-1008 5.25 MAY 19965.5.6 Reagent Aspiration Unit 5.5.6.1 Outline Reagent aspiration unit aspirates the diluent and the dye into the main unit and monitors the presence of the reagent. Waste Chamber-3 vag, vas. PCB No, 3048. (Sensor Board) Dye Diluent Bottle Bottle svis mute i. [oye | usine| Volumetic Syringe} Figure 5-42. Outline of Reagent Aspiration Unit 5.5.6.2 Specifications and Functions (1) This unit aspirates the diiuent and the dye from the reagent botte to the hydraulic system. (2) This unit monitors the presence of the diluent and the dye. {@) This unit prevents bubbles in the hydraulic lines from being generated when the diluent or the dye becomes empty. 5.5.6.3 Arrangement of Each Parts ‘Symbol | Desorption ‘Symbol _| Description 4 | Manifold MV No. 14 Assy 4 ‘Rubber Joint No. 13. 2 | Reagent Sensor No. 1 Assy [15 ‘Sensor Block No. 5-8 3 ‘Tube Support No. 10 6 ‘Sensor Block No. 5-A. Figure 5-43 Reagent Aspiration Unit Assembly UF-100 SM 526 MAY 1996'< Reference > [Dye line tubing is covered with a black tubing. This isto prevent the dye reagent from deforming and changing the fluorescence intensity. When the dye is deformed by the light, color changes to a dark violet. 5.5.7 Incubation Unit No. 12 5.5.7.1 Outline Incubation Unit No. 12 heats the sheath fluid to constant temperature (35°C) and supplies it to the flow cell. ‘Waste Chamber-1 (@ubble Removal — JP To Few col! Heater Thermal Fuse rere Li Poe a Figure 5-44 Outline of incubation Unit No. 12 5.5.7.2 Specifications and Functions (1) Heater Function: ‘Sheath fluid is heated to 35 + 2°C, (Room temperature: 15 - 30°C) (2) Protection Function: ‘Thermal fuse of 76°C. {@) Total Capacity: Approx. 40 mL. (4) intial Warm-up Time: 16 minutes or less. 5.5.7.8 Arrangement of Each Parts ‘Symbol | Description ‘Symbol_| Description ] 4 ‘Thermistor No. 33 Assy 3 Earth Nipple No. 24 Assy 2 Heater No. 18 Assy 4 ‘Thermistor No. 34 Assy. Figure 6-45 Incubation Unit No. 12 Assembly UF-100 5M 527 MAY 1996= Reference > Temperature of the sheath fluid which flows into the unit depends on the room temperature. Thermistor No. 33 is built-in the inside of the unit to control the temperature of the unit to 35°C. Thermistor No. 34 is installed outside the unit and monitors the room temperature to compensate the sheath fluid temperature. (Adjustable for| the room temperature of 15 - 30°C). UF-100 5M 528 MAY 1996