Professional Documents
Culture Documents
A Microbiological Analysis
Danica G. Pactoranan
Marianne M. Gerong
Madelyn T. Beringuel
Jerianlou C. Cabello
Practical Research II
SY. 2023-2024
Chapter 1
Introduction
In rural areas, the traditional way to access groundwater is by installing a hand water
pump or a hand-dug well with an operating depth typically ranging from 15 to 45 meters,
although depths of up to 100 meters are possible (Brikke, François, Bredero, et al., 2003).
The water hand water pump is a common instrument used by many Filipinos to obtain
underground water, which serves various purposes such as drinking, washing clothes,
cleaning food items like fruits, raw meats, and vegetables, and even bathing. However, water
contamination remains a significant issue that Filipinos experience in their daily lives,
particularly in rural areas where water is primarily sourced from hand water pumps, streams,
or springs without adequate treatment. In the Philippines, out of a population of 109 million
people, 57 million individuals (52% of the population) lack access to a reliable, safely
managed source of water, and 43 million people (39%) lack access to safely managed
household sanitation facilities (water. Org, 2022). In the countryside, specifically in Brgy.
Alegre, many residents rely on hand water pumps due to their reliability, simplicity,
2024, there are a total of 34 hand water pumps in Brgy. Alegre has 30 private hand water
pumps and 4 public hand water pumps. However, there is a significant portion of the
population that may not be aware of the potential biological contaminants present in their
water. In this matter, some of these hand water pumps have not undergone thorough
examination to assess the microbiological organisms that may be present in the water, which
is crucial, especially when the water is used for drinking and food preparation.
Hand water pumps are a simple and effective way to access drinking water in rural
areas around the world and it is one of the most efficient sources of water. The hand water
pump is a mechanical device that pumps water from underground through manual manpower
provides a cost-free source of water that we mainly use for drinking water, sanitation, and
many more. It is a traditional way of withdrawing water from underground through a hand-
dug well. Its most important components are the piston, piston valve, handle, suction lift, and
water outlet (pumpsworld. net,2022). Hand water pumps work well depending on their
location and the depth of the hand-dug where the underground water will pump to the
surface. Its mechanism involves moving the handle in an up-and-down motion in which by
then, underground water will be hauled into the body of the hand water pump and out in the
spout.
The history of hand water pumps dates back to 200 BC. The great mathematician,
Archimedes, invented the first known hand water pump to remove seawater from a naval ship
(antler. nyc,2021). By the 1400s, hand water pumps were used in Europe during the Industrial
Revolution from 1760 to 1840 (aymcdonald,2023). However, the first successful hand water
pumps were invented by Thomas Savery in the year 1698 (mechanicalboost. com,2007).
A lot of literature has been published about hand water pumps, especially their uses
and benefits to households and communities. According to the World Health Organization
(2014), hand water pumps are proven to be safe as a water supply in households. Hand water
pumps are used not only in households but also in raising livestock, industrial are, and most
Bruni and Spuhler (2019), hand water pumps can be a great source of accessible water supply
in a community and can be a profitable source of water in a water shortage. According to the
Asian Journal of Multidisciplinary Research and Review (2021), hand water pumps can also
be helpful for farmers especially when the dry season falls on farming seasons. Furthermore,
according to the case study of Njalam’mano (2007) On “Current Status and Management of
Hand Pump Equipped Water Facilities in Blantyre Rural District, Malawi: Case Study of
Kapeni and Lundu Traditional Authorities", hand water pumps were the greatest help that the
country needs on the increase of accessible safe drinking water. On the other hand, according
to "A Study of Water Pump Efficiency for Household Water Demand at Lubuklinggau
(2017)", one of the weaknesses of water pumps is their maintenance. Whenever a hand water
pump is not properly maintained, it can cause corrosion, resulting in increased contaminants
in water.
Though a lot of literature has been presented, there has been a lack of research studies
that talk about the actual functionality and performance of hand water pumps. There is also a
lack of research paper that considers other factors on why not properly maintained hand water
pumps can increase water contamination. There is also a significant amount of research
papers regarding electric-operated hand-water pumps but a lack of research studies about
piston pumps, the hand water that is commonly installed in rural and suburban areas. There is
also insufficient data on the number of households that have hand water pumps in Brgy.
Alegre nor on the status of water hand water pumps in Brgy. Alegre.
specific hand water pumps in Brgy. Alegre is significant as this study aims to evaluate the
safety of water from these pumps for consumption, determine the contaminants present, and
also identify the microbiological level of water from hand water pumps. Communities can
use this data to advocate for improvements in their water supply and develop interventions on
how to easily maintain hand water pumps so they will not lead to corrosion. It empowers
them to take action to ensure clean and safe water access for all. In addition, the findings of
this study can help raise awareness among community members about the importance of
clean and safe drinking water. It can also provide an opportunity to educate them about the
potential risks of contaminants by maintaining their hand water pumps and ways to ensure
water safety.
1. What kinds of microbes are present in the water samples of the hand water pump?
2. What is the most probable number of fecal counts of water samples from a hand water
3. What is the most probable number coliform count of water samples from a hand water
4. Is there a significant difference between the privately installed hand water pump and the
5. What is the overall microbiological level of hand water pumps of Brgy. Alegre?
This study aims to focus on the water quality assessment of 2 publicly installed hand
water pumps and 2 privately installed hand water pumps, 4 hand water pumps in total will be
selected in Brgy. Alegre. This study only limits the hand water pumps in Brgy. Alegre and
will not include the hand water pumps outside Brgy. Alegre. The researchers will gather 320
mL of water samples in the 4 selected hand water pumps in Brgy. Alegre and will not exceed
that amount of water samples. Lastly, this study will be conducted during the whole final
term.
The study's main purpose is to assess the overall contamination level of hand water
pumps in Brgy. Alegre. The finding of this study will be useful and can potentially be
1. Government Officials
-it can provide information regarding the water quality from Brgy. Alegre from water
which they can identify potential health risks associated with water consumption and
2. General Practitioner
-it can give doctors a better understanding of the potential health risks of waterborne
diseases.
3. Household Citizens
-this study will benefit the people who live in households with installed hand water
pumps because it gives information relating to the water quality of the hand water pump that
Chapter II
Review of Literature
This literature review focuses on the assessment of water quality in hand water pumps
located in Brgy. Alegre. It discusses multiple publications that highlight the microbial
presence in water hand water pumps, the average count of fecal, and the average count of
coliform. Additionally, it also examines the significant difference between publicly installed
hand water pumps and privately installed hand water pumps in terms of their microbiological
level and microbiological level of water from hand water pumps. This chapter also identifies
Many kinds of microbes in the water can pose a significant threat to human health,
aquatic ecosystems, and the environment. These microbes can enter our water sources
agricultural runoff, and the natural weathering of rocks and minerals (nrdc.org.com, 2001).
Most importantly, hand water pumps are susceptible to these contaminants. Water
ecological imbalances, and the degradation of water quality for domestic, agricultural, and
industrial use. Arsenic contamination in groundwater is a major concern in Bangladesh, as
and Remediation" by Md. Safiuddin and Md. Karim (2001). The study reveals that arsenic
contamination has had severe effects on human health, and it also presents several measures
(2006) states that 85 million people are at risk from arsenic contamination in drinking water
and food crops in Bangladesh. In Pakistan, a study titled Drinking Water Quality and Human
Health Risk in Charsadda District, by Khan et al. (2013) investigated the level of
contamination in a specific area and found that the water was contaminated with anions,
heavy metals, and coliform bacteria. The residents of the study area reported suffering from
various health problems due to the consumption of contaminated water. The provision of safe
Inappropriate sewage and solid waste disposal, overuse of agrochemicals, and inadequate
pipe and transportation infrastructure maintenance are major sources responsible for water
contamination (doh.wa.gov, 2016). In the Salem district, the concentration and health hazards
of various contaminants were studied in the drinking water, as per the findings of Assessment
of Drinking Water Quality in Salem District Using Decision Making Test and Evaluation
Laboratory Method by Chinnasamy et al. (2023). The study identified inappropriate sewage
and solid waste disposal, overuse of agrochemicals, and inadequate pipe and transportation
infrastructure maintenance as the primary sources of water contamination. They also found
The most probable number of fecal counts from hand water pumps varies significantly
depending on several factors, making a precise estimate challenging. A study by Akoto et al.
(2018) in Ghana reported fecal coliform levels exceeding the WHO guideline in 44% of hand
water pump water samples, highlighting the pervasive nature of the issue. A 2019 study by
Bain, et al. (2019) found that 57% of hand water pump water samples in rural India exceeded
the World Health Organization (WHO) guideline for fecal coliforms, indicating
contamination with fecal matter and potential health risks. Another study found that total
coliform counts in hand water pumps were significantly higher during the rainy season than
the dry season, it has shown a wide range, from <1 to 685.3 colony forming units (CFU) per
100 milliliters (mL) of water Wenbiao, Toure, 2020). These variations are attributed to
factors such as season (dry vs. rainy), proximity to sanitation facilities, well construction, and
rainfall, potentially due to surface runoff and overflowing latrines. Additionally, hand water
pumps located near latrines or other sources of fecal contamination are more likely to have
Coliform bacteria are one of the microbes that one must look out for as they can cause
coliform counts in water samples obtained from a hand water pump might exhibit significant
variation due to factors such as the geographical location, water source, and the existence of
impurities. Coliform bacteria serve as reliable indicators of water quality, as their existence
implies possible fecal pollution. Typically, the coliform count in drinking water should be
extremely low, preferably nil. The World Health Organization (WHO) and other health
authorities establish criteria for potable water. The recommendations set by the World Health
Organization (WHO) state that drinking water should not have any measurable presence of
Escherichia coli (E. coli) bacteria per 100 mL of water. In another study by Sharma, L &
Sharma, B (2018) Hand water pumps are meeting the need for drinking water in these
hand water pumps for a variety of water quality indicators, including pH, hardness, chloride,
fluoride, NO3 -N, EC, orthophosphate, TDS, TSS, BOD, total coliforms, and fecal coliforms.
According to the chemical parameter data, the water quality in all 18 hand water pumps was
within the WHO-permitted threshold. However, in eight of the hand water pumps, the fecal
coliforms were greater than the allowable limit (2-6 MPN/100 mL), proving that these water
Both public and private hand water pumps provide access to water either for
consumption or other activities however, their microbiological level may differ from each
Areas of Northern, India”, a research study done by Singh, Goel, Gulati et.al (2019), where
80 public hand water pumps were analyzed for microbiological analysis, found that 75% or
60 out of the 80 of the hand water pumps were contaminated with E.coli bacteria.
Rural Communities in Kenya, a research study done by Kirianki, Silas, Jo et.al (2015), out of
the 150 public hand water pumps that were tested for microbiological analysis, found that
68% or 102 out of 150 hand water pumps tested positive for Fecal bacteria. On the other
hand, private hand water pumps may or may not have the same microbiological analysis as
public hand water pumps. According to Hand Pump Water Quality and Association with
study done by Mckay, Culigan, Keen et. al. (2019), it was found that 41% of the water
samples from randomly selected household hand water pumps tested positive with Fecal
indicator bacteria putting some areas in rural India in a higher risk of getting diarrhea. In
support of this, according to the study by Shield, Bain, Wright et. al (2015), entitled
Association of Supply Type with Fecal Contamination of Source Water and Household
samples that were tested for microbiological analysis, 46% were tested positive with various
bacteria such as salmonella and coliform. Private and public hand water pumps may have the
same use but may have different microbiological contamination levels on the other hand,
according to Water Supply (2018), it is safe to drink water from public hand water pumps as
it is because of the purification properties and minerals of the soil underneath, however,
according to Simple Pump (2023), it can be considered safe to drink water from private hand
water pumps or household hand water pumps as long as it is manufactured and compliant
with Safe Drinking Act, where it involves ensuring that the supply used to make the hand
water pump is lead-free and the hand water pumps are regularly well-maintained.
The microbiological level of water from hand water pumps determines whether water
from hand water pumps is safe to drink or use. Hand water pumps are one of the primary
sources of water in rural and suburban areas (www.sswm.info.com, 2023), however, due to
poor sanitation and poor management, water quality, and safety are compromised. The
increasing level of bacteria and viruses in the hand water pumps can make water and water
sources inaccessible and unsafe. According to the article published in National Geographic
Society by Brown (2023) titled, “Water Inequality”, open defecation is the biggest
contributor when it comes to high water contamination levels, one example of this is Nepal.
75% of water in schools of Nepal is contaminated with Fecal Bacteria. A research study done
by Sigh, Shukla et.al (2021) called, “Management of Risk Factors for Breaking Localized
Pathways of Microbial Contamination in Tube Wells with Hand Pumps: A Case Study”,
where 154 water hand water pumps were analyzed for Bacteriological Analysis and found
that, out of 154 hand water pumps analyzed, only 48 water hand water pumps was labeled as
“safe” and 106 was labeled as “unsafe”, due to high levels of contaminants. Similarly,
Contamination Levels and Mitigation (2014)”, hand water pumps installed near sewage
systems can also contribute to high contaminants present in the water of hand water pumps
Water quality assessment in hand water pumps using microbiological analysis has
much importance as it determines the quality of drinking water supplies and assesses the
potential diseases that people can get from contaminated water. Even though there are a lot of
studies on water quality assessment in hand water pumps, there is an insufficient study in
understanding the factors that contribute to the deterioration of water quality of hand water
pumps other than the expense of maintaining hand water pumps leading it to corrosion
(doi.org, 2020). Numerous studies have been done regarding the microbiological analysis of
hand water pumps, however, there is a lack of research studies concerning which types of
hand water pumps are more susceptible to contamination. Multiple studies point out that open
defecation and poor maintenance of hand water pumps in rural areas are the factors that
contribute to water contamination however, there is a lack of research studies that are
concerned with the intervention that can reduce water contamination in rural areas. There is
no surprise that researchers tend to only focus on the community’s poor maintenance of hand
water pumps, however, researchers that studied water quality assessment on hand water
pumps fail to assess what are the necessary interventions that communities can do to maintain
Research Framework
The researchers use the input-process-output model. It includes the flow of data,
information, and materials that will be used in conducting this research. The concept model,
For input, it contains the research’s independent and dependent variables. Usually, all
For process includes the pre-survey, the water sample collection, water analysis, and
result interpretation.
In gathering the data, the researcher conducted a pre-survey of the private and public
microbiological level of Brgy. Alegre and actions that will be taken after interpreting the
Definition of Term
Fecal Count. Bacteria that are found in the guts and feces of humans and animals
(knowyourh2o.com, 2015).
Hand Water Pump. A water-lifting device operates manually to withdraw water from
surface water sources, groundwater sources, and reservoirs (sswm.info.com, 2019). In Brgy.
Alegre, hand water pumps are used for drinking water, washing, sanitation, agricultural
Coliform Count. Bacteria that are found in soil, in water are influenced by surface water and
Chapter III
Methodology
I. Research Design
A total number of 34 hand water pumps in Brgy. Alegre, Dulag, and Leyte, the
researchers will select four randomly selected hand water pumps, two private and two
public hand water pumps that are scattered in different zones in Brgy. Alegre. Each
This research study will use recording and observation as research instruments.
Result charts:
Pumps
HWP #1
HWP #2
Pumps
HWP #1
HWP #2
Table 2. Public and private installed Hand Water Pumps (Most probable number of Fecal
count)
HWP #1 HWP #1
HWP # 2 HWP # 2
Table 3. Public and private installed Hand Water Pumps (Most probable number of Coliform
HPW= Hand Water Pump
Count)
Public installed Coliform Count Private installed Coliform Count
HWP #1 HWP #1
HWP # 2 HWP # 2
Table 4. The overall microbiological level of Hand Water Pumps in Barangay Alegre.
Public
Hand
Water
Pumps
Private
Hand
Water
Pumps
The researcher conducted a pre-survey to determine the total population of hand water
pumps in Brgy. Alegre Dulag, Leyte and to also determine the water samples needed.
Furthermore, the researchers collected water samples from selected water hand pumps
and were examined using microbiological analysis, particularly Fecal count, and Coliform
Prime Waters, Tacloban with a capacity of 100 ml was cleaned and sterilized. Bottles
1. The researcher will check the hand water pump if it is still usable. Pump for three
minutes to ensure that the water sample represents the quality of groundwater that
2. After three minutes of pumping, the researchers will wipe the outlet or mouth of
the hand water- pump to remove any dirt found using a clean cloth or tissue
3. After pumping, the researchers will open the sterilized bottle and fill it with water.
4. The water bottles will be tightly sealed to exterminate any further contamination.
5. The researcher will label the bottles according to their classification- private or
public.
6. The water samples will be safely transported to the laboratory of Prime Waters
V. Data Analysis
The following statistical tools will be used in analyzing the data in each research